LOCUS       DASTZO010000001         6095 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_26143,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000001 DASTZO010000000
VERSION     DASTZO010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6095)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6095)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6095
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26143"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /gene="dacB"
                     /locus_tag="VFU20_00005"
     CDS             <1..723
                     /gene="dacB"
                     /locus_tag="VFU20_00005"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEU5284875.1"
                     /translation="AGAEPELLELGIDDPAHFAAWRLRSLLLERGVTVGGEVTARHRP
                     PMPKGERAAAPRPAGPAVLARLAGPPLLQDLKTINKLSQNVHAELMLRRVGRQEGDGS
                     IADGVAAIEAMLTSAGVPRTQYDFSDGSGMSTYNRVAPRATIRLLRWIDAQPWAAQWR
                     GTLPIAGVDGTLDERFKGTWLRGRLFAKTGTLNATNALAGYLVARSGRTLLFAIYAND
                     VPGGGSARDAMDAALVLVAEEN"
     gene            857..3832
                     /locus_tag="VFU20_00010"
     CDS             857..3832
                     /locus_tag="VFU20_00010"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5284876.1"
                     /translation="MASGTAFAALFLATAAPAQAQTSQPEEETTEPATTPTTAETPPT
                     GADVTSGPPPQAASQEIIVTGSRIRRNPNDSALPLQIITTRELERNAISNPEQLIAYL
                     PTNGTAGDNLASNADVVSGQQRGNNGASFANLRGQGSGATLILLNGRRVAAHGLQGSA
                     VDVNQIPFAAVERVEVLKEGASAIYGTDAIGGVINFILRKDYQGLGIQGFTDVAEEGD
                     HTIFRTSAIAGYGDLEENGFNVMAAVSWSDIPELRGDQRDFVNTFQPDRGLSVDTRGT
                     PHATIFALSNTFFPTAGSFPLIPGTTTLASGGLNVLDLPGAEGCDSIDGMGPYDPVLW
                     AVPTAALACAWDTGRAAVLQQPLETLTWLGRGVARLGEHEIVGEITGSDATAAKRFSH
                     LQITPNTTSQRYEHPVGAPGYDEVYNRLIAAFPTLATRPRDGFAYRWRCIECGRREIT
                     TDTKTMRYALGADGPLFGGWDYRTGASYATSESQSELGTGYYYRGTLASGAPDPTAPT
                     APGAPGPGIIGLLNSGLINPFLLPGQTQSKAALDALAAVSAEGVILYGGKYSTMQVDG
                     SVSGSLFELPGGTVRAALGVDYRKEKYRFNGDEREALARPTIIAAPFDDANALEGVSR
                     SIKAAYGEVLLPILDGLEVTLAGRIDDYSDFGTTENPMVSFKYRPMRQLMLRGNYNTG
                     FRAPTFNQLFNGRVESLFTGADLADPIKCPGGIPNTTDPNCASLARAVDIISGGNPDL
                     GPETAKMASLGIVFEPAPNFSASVDWWTINRKDTIQLFTLNQVKHRDNAPLLERLFIR
                     DSAGNLTAIDLSWVNIGGSKTQGIEIALRGAGRLLGGRVSGGLDGTYLLKKKEQLIEG
                     GPFENRRGVFSFSGDLGLKWKHNAFVSYNRGALTTSLTQVWRSGSRNQQLPGVLSGAI
                     DPPNDEKRVDKYVIYNLSAAMEVTDRLRITAGIKNLFDKDPPFAIAYDSNTGSGSSWE
                     PRVADPRGRSFTMNVDVKF"
     gene            complement(3877..3950)
                     /locus_tag="VFU20_00015"
     tRNA            complement(3877..3950)
                     /locus_tag="VFU20_00015"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3916..3918),aa:Cys,seq:gca)
     gene            4148..4804
                     /locus_tag="VFU20_00020"
     CDS             4148..4804
                     /locus_tag="VFU20_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HEU5284877.1"
                     /translation="MTVQLPIPDFAAARRTMIDNQLRPVGVTDPLVLEAMGSVARERF
                     VLEDNRPLAYADRSLPIGGGRFLAAPAVLGQLLTQMAPKPGELALVVGAGTGYTAAVL
                     GAIGCEAVALESSAELAARARELGVETAEGPLEAGHKAGAPYDLILIDGAVETIPDAI
                     VKQLAERGRLGAALAEGGITRLIVGQKVAGAFGYLSIADAGVAALPGFSRPRGFRFER
                     "
     gene            4810..>6095
                     /locus_tag="VFU20_00025"
     CDS             4810..>6095
                     /locus_tag="VFU20_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HEU5284878.1"
                     /translation="MLRRLALASLAAALMAGTASADTLREALLSTYRTNPTITGQREA
                     LRVTDANVAIARAAGRPSVSATAGLNRDLTRSGILDTGGKGPDLSVGVDLSYPLFSGG
                     SVRNSVRAAETRVEAGRATLRAVEGDVFTEAVAAYMDVIRDRAIVELNANNVRVLGTN
                     LEATRDRFEIGDLTRTDVAQSEARLQLGRSNLATAQGRLTGSEENYRRVIGKRPDALA
                     PPPPLPPLPATPDEAVRIALASNPDLVAVTRQASAAGLDVRVARAGRLPTVSGVVSGD
                     YINALGGNEGPLPSSGTQTSAGLNARIPLYQGGLPAARIRAAQALEGQFLEQMVATER
                     AVVSNTRSAFASYQAAQNAIASNEVAVRANELALEGARAEQSVGTRTVLDVLNAEQEL
                     LNSQVALVTARRDAYVAGFQLLNAMGQAEADDLGLEG"
BASE COUNT          995 a         2160 c         2086 g          854 t
ORIGIN      
        1 gccggcgccg agcccgagct gctcgagctc gggatcgacg acccggcgca ctttgccgcc
       61 tggcgcctgc ggtcattgct gctcgagcgg ggagtaacgg ttggcggcga agtcacggcc
      121 cggcaccgcc cgccgatgcc caagggcgaa cgggccgccg ccccgcgccc cgccgggccg
      181 gccgtgctcg cgcgactggc cggcccgccg ctgcttcagg acctcaagac catcaacaag
      241 ctcagccaga acgtccacgc cgagctgatg ctgcgccggg tcgggcggca ggagggcgac
      301 ggctcgatcg ccgacggcgt cgcggcgatc gaagcgatgc tgacgagcgc cggagtgccg
      361 cggacgcagt acgacttttc cgacgggtcg ggcatgtcga cctacaaccg ggtcgccccg
      421 cgcgccacca tccggctgct gcgctggatc gacgcgcagc cctgggccgc gcagtggcga
      481 ggaacgctgc cgatcgccgg ggtcgacggg actctcgacg agcggttcaa ggggacttgg
      541 ctccggggac ggctgttcgc caagaccggg accctcaacg cgaccaatgc gcttgccggc
      601 tatctggtcg ccaggagcgg ccggaccctg ctgtttgcga tctacgccaa cgacgtcccg
      661 ggtggcggca gcgccaggga cgcgatggac gccgcgctgg tcctggtcgc cgaggaaaat
      721 tgacctcccg gaagcggctt gtcgcaaccg tttctggagg ctgaagaaat cgcttcggcc
      781 taaggagccc ttttgttaca caaatcggac tcgagggcag gggggcacca tggtcgtttc
      841 gcgattcaaa accgttttgg cttcgggcac cgcgttcgcg gcgctgttcc tggcgacggc
      901 cgcgcccgcg caggcgcaga ccagccagcc ggaggaggag acgaccgagc cggcgacgac
      961 tccgaccacc gccgagactc cgccgaccgg cgcggacgtg accagcggcc ctccgcccca
     1021 agcggcgagc caggagatca tcgtcaccgg ctcgcggatc cggcgcaacc cgaacgacag
     1081 cgcgctgccg ctgcagatca tcaccacccg cgaactcgag cgcaacgcga tcagcaaccc
     1141 cgagcagctg atcgcctacc tgccgaccaa cggcaccgcc ggcgacaatt tggccagcaa
     1201 cgccgacgtc gtcagcggcc agcagcgcgg caacaacggg gcctcgttcg ccaatttgcg
     1261 cgggcagggg tcgggtgcga ccctgatcct gctcaacggc cggcgcgtcg ccgcccacgg
     1321 cctccagggc tcggcggtcg acgtcaacca gatcccgttc gccgcggtcg agcgggtcga
     1381 ggtgctcaag gaaggcgcgt cggcgatcta cggcaccgac gcgatcggcg gggtgatcaa
     1441 cttcatcctg cgcaaggatt accagggcct cggaatccag ggtttcaccg atgtcgccga
     1501 ggagggggat cataccatct tccgaacctc cgcgatcgcc ggctacggcg acctcgagga
     1561 gaacggcttc aacgtcatgg ccgccgtcag ctggagcgac atccccgagc tgcgcggcga
     1621 ccagcgcgat ttcgtcaaca ccttccagcc cgaccgcggc ctgtcggtcg acacccgcgg
     1681 aacgcctcac gcgaccatct tcgcgctctc caataccttc ttcccgaccg ccgggtcgtt
     1741 ccccttgatc cccggcacca ccaccttggc cagcggcggc ctcaacgtgc tcgacctgcc
     1801 cggggccgag ggttgcgact ccatcgacgg aatgggcccc tatgaccccg ttctgtgggc
     1861 ggtgccgacg gccgcgctcg cctgcgcctg ggacacgggg cgtgcggcgg tgctgcagca
     1921 gccgctggag acattgacct ggctcggccg cggggtcgcg aggctgggcg agcacgagat
     1981 cgtcggcgag atcaccggct ccgacgcgac cgcggccaag cgcttttcgc acctgcagat
     2041 cactcccaac acgaccagcc agcgctacga gcatccggtc ggcgcgcccg gctatgacga
     2101 ggtctacaac cggctgatcg ccgcctttcc gaccctggcg acccggccga gggacggctt
     2161 tgcctaccgc tggcgctgca tcgagtgcgg gcggcgcgag atcaccaccg acaccaagac
     2221 catgcgctac gcgctgggcg ccgacgggcc gctgttcggc ggctgggact atcgcaccgg
     2281 cgcatcctac gcgaccagcg agtcgcagtc ggagctcggc accggctatt attaccgcgg
     2341 cactctggcg agcggcgctc ccgatcccac cgcgccgacc gctcccgggg cgcctgggcc
     2401 cggaatcatc ggccttctca acagcgggtt gatcaacccg ttcctgctgc ccggccagac
     2461 ccagtcgaag gcggcgctgg atgcgctcgc ggcggtgtcg gcggaagggg tcatcctcta
     2521 cggcggcaaa tattcgacta tgcaggtcga cggctccgtg tccggatcgc tgttcgagct
     2581 tcccggcgga accgtccggg cggcgctggg ggtcgattac cgcaaggaaa aataccgctt
     2641 caacggcgac gagcgcgagg cgctcgcacg gccgacgatc atcgccgcgc cgttcgacga
     2701 cgccaatgcg ctggaaggcg tgtcgcgcag catcaaggcg gcttacggcg aggttctgct
     2761 gccgatcctc gacgggctcg aggtgacgct ggccggccgg atcgacgatt attccgattt
     2821 cgggacgacc gagaacccga tggtgtcgtt caagtaccgg ccgatgcggc agctgatgct
     2881 gcgcggcaat tacaacacgg gcttcagggc gccgaccttc aaccaattgt tcaacggccg
     2941 ggtggaatcg ctgttcaccg gcgccgacct cgccgacccg atcaaatgcc ccggcggcat
     3001 ccccaacacc acggacccca attgcgcgtc cctggccagg gcggtcgaca tcatcagcgg
     3061 cggcaacccg gacctcggcc cggaaaccgc caagatggcg agcctcggaa tcgtcttcga
     3121 gccagcgccc aatttcagcg cctcggtcga ctggtggacg atcaatcgca aggacacgat
     3181 ccagctcttc accctcaacc aggtcaaaca ccgcgataac gcgcccttgc tcgagcggct
     3241 gttcatccgc gactcggccg gcaatttgac cgccatcgac ttgagctggg tcaacatcgg
     3301 cgggtcgaag acgcagggca tcgagattgc cttgcgcggc gccgggcggc tgctcggcgg
     3361 gcgggtgtcg ggaggcctcg acggcaccta tctgctcaag aagaaggagc agctgatcga
     3421 aggcggcccg ttcgagaacc gccgcggagt cttctcgttc agcggcgacc tgggcctcaa
     3481 gtggaagcac aacgccttcg tcagctacaa tcgcggggca ttgaccacgt cgctgaccca
     3541 ggtctggcgc tcgggctccc ggaaccagca attgcctggg gtcctcagcg gagcgatcga
     3601 cccgcccaac gacgagaagc gggtggacaa atatgtgatc tacaatttgt cggcggccat
     3661 ggaggtcacc gaccggctgc ggatcaccgc gggcatcaag aacttgttcg acaaggaccc
     3721 gccgttcgcc atcgcctacg acagcaacac cggcagcggc agctcgtggg agccgcgcgt
     3781 cgccgacccg cgcgggcggt cgttcacgat gaacgtcgac gtcaagttct gaaggcggcg
     3841 cggccccggg gccggtaagc gcggtgctga gaagagtgga ggcccgagcc ggaatcgaac
     3901 cggcgtgcaa ggatttgcag tcctctgcgt aaccactccg ccatcgggcc gaggcgcgcc
     3961 atgtcggggg cttcgcgccc ggcgtcaagc atcaacccgc cctggaattc aactccattc
     4021 cccgcagcgg gccgttggag cgattgaccg cagccgctcg tcgttcgacc aacggcggtt
     4081 ggcgcggcgc caggctgccg gtaagagcgg ccttgcatcc caggtgtgtt agcggcatat
     4141 aacagctatg acggtccagc ttccgatccc cgacttcgcg gccgcgcgcc gaacgatgat
     4201 cgacaaccag ctgcgcccgg tcggagtcac cgaccccctg gtgctcgagg cgatgggatc
     4261 ggtcgcccgc gagcgcttcg tgctcgagga caaccggccg ctggcttatg ccgaccggtc
     4321 gctgccgatt gggggcgggc gcttcctggc ggcgccggcc gtgctcggcc agctgctgac
     4381 ccagatggcg cccaagccgg gcgagctcgc gctcgtggtc ggagccggca ccggctacac
     4441 ggcggcggtg ctcggcgcga tcggctgcga ggccgtggcg ctcgaaagct cggccgagct
     4501 cgccgcccgc gcccgcgagc tcggggtcga aaccgccgag gggccgctcg aggcgggcca
     4561 caaggccggc gcgccctacg acctgatcct gatcgacggg gccgtcgaaa ctatcccgga
     4621 cgccatcgtc aagcagctcg ccgagcgcgg gcggctcgga gcggcgctcg ccgaaggcgg
     4681 catcacccgg ctgatcgtcg gccagaaggt cgccggggcc ttcggctatc tgtcgatcgc
     4741 cgacgccgga gtcgccgctc tgccgggatt ctcgcggcca cggggcttca ggttcgaaag
     4801 gtgagaagcg tgttgcgcag gcttgcactg gcgtcgctgg cggcggcgct gatggcgggg
     4861 accgcatcgg ccgacacgct gcgcgaggcc ctgctgtcga cctaccggac caatcccacg
     4921 atcaccggcc agcgcgaggc gctgcgggtg accgacgcca atgtcgcgat cgcccgagcc
     4981 gccggccggc cgtcggtcag cgcgaccgcg gggctcaacc gcgacctgac ccgaagcggc
     5041 atcctcgaca ccggcggcaa gggccccgac ctgtcggtcg gagtcgacct cagctaccca
     5101 ttgttcagcg gcggcagcgt ccgcaacagc gtccgggccg ccgagacccg ggtcgaggcg
     5161 gggcgggcga cgctgcgcgc ggtcgagggc gacgtgttca ccgaggcggt cgccgcctac
     5221 atggacgtga tccgcgaccg ggcgatcgtc gaactcaatg ccaacaacgt gcgggtgctg
     5281 ggcaccaacc tcgaggcgac gcgcgaccgc ttcgagatcg gcgatctgac ccgaaccgac
     5341 gtcgcccagt ccgaagcgcg gctgcagctc ggccggtcga acctcgccac cgcgcagggg
     5401 cggctgactg ggagcgagga gaattaccgg cgggtgatcg gcaagcgccc ggacgcgctg
     5461 gcgccgccgc cgccgctgcc gccgctgccg gcgacccccg acgaagcggt gcgaatcgcg
     5521 ctcgccagca acccggacct ggtcgccgtc acccgccagg cttcggccgc cgggctcgat
     5581 gtccgggtgg cccgggcggg acgcctgccg accgtgtcgg gagtggtcag cggcgactac
     5641 atcaatgcgc tgggcggcaa cgagggaccg cttccgagca gcggcaccca gaccagcgcc
     5701 gggctcaacg cccgcatccc gctctaccag ggcgggcttc cggcggcccg aatccgcgcc
     5761 gcccaggcgc tcgagggcca gttcctcgag cagatggtcg ccaccgagcg ggcggtggtc
     5821 tcgaacaccc ggtcggcgtt cgccagctac caggcggcgc agaacgcgat cgcgtcgaac
     5881 gaagtggcgg tccgggccaa cgagctggct ctggaaggcg cgcgggccga gcagagcgtc
     5941 ggcacccgca ccgtgctcga cgtcctcaac gccgagcagg agctgctcaa ctcgcaggtc
     6001 gcgctggtga ccgcccggcg ggacgcctac gtcgccggct tccagctcct gaacgcgatg
     6061 ggccaggccg aggccgacga cctcgggctc gaggg
//
LOCUS       DASTZO010000002         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1149124,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000002 DASTZO010000000
VERSION     DASTZO010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149124"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="VFU20_00030"
     CDS             <1..584
                     /locus_tag="VFU20_00030"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU5284879.1"
                     /translation="LALARELRDRLVERGRVRVALTRDSDRYLTLEERAGVARGLGAS
                     LFLSLHMDSAPNPLARGATVYSLSDVASDAEAARFAEAENAGGAAGATDGSVRSMLSD
                     LALRSLMSASADLATRLVRKSAARVEMRPEPHRFATFHVLRRAEAPAVLFEAGYVSNA
                     DDELLLRSPDHRAALVAGLAETIEADVAARARR"
     gene            642..>2964
                     /locus_tag="VFU20_00035"
     CDS             642..>2964
                     /locus_tag="VFU20_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU5284880.1"
                     /translation="MKFAKIDPRRLTVPDLRLPDLVGFNRRVHDRIDPWFERRWVRRG
                     AWIGGVGFLFFAAVWLFFATGLPTSEKLLAYQPPLPTNVRGYDGNPVQTFARERRVEL
                     AYDEYPPLVIHAFLSAEDKTFFRHGGIDYPGLVGAVFDFTRKSLTSGERARGGSTITQ
                     QVAKALLQDSSYSIGRKIREAILAFRLESTLTKEQILELYLNQIFLGRNAYGVQAASR
                     AYFDKDVGELTLPEAAYLAVLPKAPSNYDPVRATGRAVARRNYVLREMVSNGYITPEQ
                     MAAAAATSLGTIRYGSNAKFREMGGYFMEEVRRDLIRRFGEEADEGPNSVYAGGLWVR
                     TSVVPHMQAAAAEALREGLAKFDGGRGWRDPGLSVDISSDWAGQLDRAPVGTGFPDWR
                     KAVVLSKTGGEARIGFANGSTGILPASAASMPKRGEGGAAFTHLRPGMIIVVKKIAGD
                     DYALRSIPEIGGGFVAEEVRTGRVLAMQGGFDVIGSSYNRATQALRQPGSAFKPIVYE
                     TALENGMTPASIIVDSPFCVWQGAGLGNKCFVNFDRRYSGPKTMRWGVEQSRNLMTVR
                     AASQTGIAKVTDTARKLGVGDYPDYLSIALGAGDTTVLKLTNAYAILANHGRSVRPTL
                     IDFIQDRNGKVIFRTDNRCQIMDNCSAAEWDGKGMPRPPVRTRQLLDPMAAFQMVHIL
                     TGVVERGTATVLRDLDRPLFGKTGTTSGPTNVWFVGGTPDVVAGVYLGYDQPRSLGGY
                     AQGGRIAAPIFKQWAKIALASQPKTPFVAPAGIR"
BASE COUNT          443 a         1074 c          997 g          450 t
ORIGIN      
        1 cgctggcgct ggcgcgcgag ctccgcgacc ggctggtcga gcgcggccgg gtccgcgtcg
       61 cactgacccg cgacagcgac cgctacctga cgctcgagga gcgggccggg gtcgcccgcg
      121 ggctcggcgc cagtctgttc ctgtcgctgc acatggacag cgcgcccaac cccctggccc
      181 gcggtgcgac cgtttattcg ctgtccgacg tcgcgtcgga cgccgaggcg gcccggttcg
      241 ccgaggccga gaatgcgggc ggggccgccg gggccaccga cggatcggtc cgctcgatgc
      301 tttccgacct tgcgctgcgc agcctgatga gcgcgtcggc cgatctcgcc acgcggctgg
      361 tccgcaagtc ggcggcgcgg gtcgagatgc gccccgagcc gcaccgcttc gcgaccttcc
      421 acgtgctccg ccgggccgag gcgccggcgg tgctgttcga ggccggctac gtcagcaatg
      481 ccgacgacga gctgctgctg cgctcgcccg atcatcgagc ggcgctggtc gccgggcttg
      541 ccgagaccat cgaagccgac gtcgccgcgc gcgcgcgccg ctaagggccg ccgctgatcc
      601 ggctttgcgg gcctcgccga accggctaga tgcctgccca aatgaaattc gccaagatcg
      661 atccaaggcg cctgaccgtt cctgacctgc ggctgcccga cctggtgggg ttcaaccgcc
      721 gggttcacga ccgcatcgat ccctggttcg agcgccgctg ggtgcgccgc ggcgcatgga
      781 tcggcggagt cggcttcctg ttcttcgccg cggtctggct gttcttcgcc accggcctgc
      841 cgacctccga gaagctgctc gcctaccagc cgccgctgcc caccaacgtg cgcggttacg
      901 acggcaaccc ggtgcagacc ttcgcccgcg agcggcgggt cgagctcgcc tatgacgaat
      961 atccgccatt ggtcatccac gcgttcctgt cggccgagga caagaccttc ttccgccacg
     1021 gcggcatcga ctatccgggc ctggtcgggg cggtgttcga cttcacccgc aagagcctga
     1081 ccagcggcga gcgggcgcgc ggcggctcga ccatcaccca gcaggtggcg aaagcgctgc
     1141 tccaggacag cagctactcg atcggccgca agatccgcga ggcgatcctc gccttccggc
     1201 tcgaatcgac gctgaccaag gaacagatcc tcgagctgta cctgaaccag atcttccttg
     1261 gccggaacgc ctatggagtg caggcggcga gccgcgccta tttcgacaag gacgtcggcg
     1321 agctgacgct tcccgaagcg gcctatctcg cggtgcttcc caaggcgccg tccaactacg
     1381 acccggtgcg ggcgaccggg cgggcggtcg cgcgccgcaa ctacgtgctc cgcgagatgg
     1441 tcagcaacgg ctacatcacg cccgagcaga tggccgccgc cgccgccacg tcgctcggca
     1501 ccatccgcta tggaagcaac gccaagttcc gcgagatggg cggctatttc atggaggagg
     1561 tgcgccgcga cctgatccgg cgcttcggcg aggaggcgga cgagggcccc aacagcgtct
     1621 atgccggcgg cctgtgggtc aggacctcgg tggtcccgca catgcaggcg gccgccgccg
     1681 aggcccttcg cgaaggcctc gccaagttcg acggcggccg cggctggcgc gacccgggcc
     1741 tcagcgtcga catctcgagc gactgggcgg ggcagctcga ccgggcgccg gtcgggaccg
     1801 gctttcccga ctggcgcaag gcggtcgttc tgtcgaagac cggcggtgaa gcgcggatcg
     1861 gctttgccaa cggctcgacc ggcattcttc cggcgtccgc cgcctcgatg cccaagcgcg
     1921 gtgagggcgg cgccgccttc acccatctgc ggccgggaat gatcatcgtc gtcaagaaaa
     1981 tcgccggcga cgattacgcg ctgcgctcga tcccggagat cggcggcggc ttcgtcgccg
     2041 aggaggtccg caccggccgg gtgctggcga tgcagggcgg cttcgacgtc atcggctcga
     2101 gctacaaccg ggcgacccag gcgctccgcc agccgggctc ggcgttcaag ccgatcgtct
     2161 acgagaccgc gctcgagaac ggcatgaccc cggcttcgat catcgtcgat tcccccttct
     2221 gcgtctggca gggggcgggg ctcggcaaca aatgcttcgt caatttcgac cgccgctatt
     2281 cggggcccaa gacgatgcgc tggggggtcg agcagtcccg caacctgatg accgtccggg
     2341 ccgcctcgca gaccggcatc gccaaggtca ccgacaccgc ccgcaagctc ggggtcggcg
     2401 actatcccga ttacctctcg atcgcgctcg gcgccggcga caccacggtg ctcaagctga
     2461 ccaacgccta tgcgatcctc gccaaccacg ggcgctcggt gcggccgact ctgatcgact
     2521 tcatccagga ccgcaacggc aaggtcatct tccggaccga caaccgctgc cagatcatgg
     2581 acaattgcag cgccgccgaa tgggacggca agggcatgcc gcggccgccg gtgcgcaccc
     2641 gccagctgct tgacccgatg gccgcgttcc agatggtcca catcctcacc ggggtcgtcg
     2701 agcgcggcac cgcgaccgtt ctccgcgacc tcgaccggcc gctgttcggc aagaccggga
     2761 ccacctctgg cccgaccaac gtgtggttcg tcggcggcac tccggacgtc gtcgccgggg
     2821 tctacttggg ctatgaccag ccgcgcagcc tcggcggcta cgcgcagggc gggcggatcg
     2881 cggcgccgat cttcaagcaa tgggcgaaaa tcgcgcttgc gagccagccc aagaccccgt
     2941 tcgtcgcccc ggcgggcatt cgct
//
LOCUS       DASTZO010000003         2897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1122998,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000003 DASTZO010000000
VERSION     DASTZO010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1122998"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VFU20_00040"
     CDS             complement(<1..907)
                     /locus_tag="VFU20_00040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5284881.1"
                     /translation="MSPDPQLAEALAAFQRGDLDQAQRLAEGLATGAPSPAIDHLLGL
                     IHCRRGDPGSGVEHLRRALEAEPGNAAYRLILSRALVDAGRAAEALALPRPEPSAAPA
                     GLALWHARAEAADAAGDARAAKEAWEVIAAARPADGRAWNNLGNALAALSDWQGAADA
                     LGRAAELNPAEAPIRRNLAVALARLERFEDSAGQLREAARLDPGDLETRLTLARLLAD
                     LDRHEEALAQLEQASALAPGSFEVAIARGRSLVALSAFDEAERAYEAALATSPADRTA
                     FHELGLLLERTNRMDALRDLLGRATG"
     gene            complement(963..1817)
                     /locus_tag="VFU20_00045"
     CDS             complement(963..1817)
                     /locus_tag="VFU20_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5284882.1"
                     /translation="MSDIVEPMIHEDALPGHESQLQPKPDWEPRYKGSDRLKGKVALI
                     TGADSGIGRAVAALFAREGADVAILYLCEHDDANKTKAIVEKEGRKAIAIPGDIGDKS
                     FCERAVAQTVQQLGRLDILVNNAGEQHPDKDIRDITEDQLKRTFQTNIFGMFFMTQAA
                     RPHLKKGSAIINCTSVTMYKGSDDLLDYSATKGAITAFTRSLSQNLVKDGIRVNAVAP
                     GPIWTPLNPMGGQPPEDMKEFGKSVPMGRPGQPNEVAPSFLFLACEDASYMTGQTLHP
                     NGGVVVNG"
     gene            1909..>2897
                     /locus_tag="VFU20_00050"
     CDS             1909..>2897
                     /locus_tag="VFU20_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEU5284883.1"
                     /translation="MASAAQITERRSGSFRTLLRFLPMLWPKGEGELKARVVAAVLLV
                     LAGKAAVLLMPFAYKAVIDRMSAGTAVFAVVAGLVVAYSVARFAGVLADNLRNALFEK
                     VGQRAARKLAGRVFRHVHDLSLRFHLERRTGSLTKIVERGTKSIDMMLYFLLFNIAPT
                     VIELAAICVIFFVKFGPGLVAATLAMVAVYIVFTRKVTDWRARVQREMNEVDNRAIGR
                     AVDSLLNYETVKYFGAEEREARRYDEAIGAYADAATKNETSLAWLNIGQSLITNLMMA
                     GAMIFTVWGWSQGRFTPGDVVFINTMLLQLFRPLDMLGWVYRSIRQGLIDMEA"
BASE COUNT          403 a          991 c         1022 g          481 t
ORIGIN      
        1 cgcccgtcgc ccggccgaga aggtcgcgga gagcgtccat ccggttggtg cgctcgagca
       61 gcaggccgag ctcgtggaac gcggtccggt cggcgggcga cgtggcgagg gcggcctcat
      121 aggctcgttc cgcttcgtcg aaggcgctca gcgcgaccag gctgcggccg cgcgcgatcg
      181 ccacctcgaa gctgcccggc gcgagcgccg acgcctgctc gagctgcgcc agcgcctcct
      241 cgtggcgatc gagatcggca agcagccggg caagggtcag ccgggtctcg agatcgcccg
      301 gatcgagccg ggcggcctcc cggagctgcc cggcgctgtc ctcgaaccgc tccagcctgg
      361 cgagcgccac cgccaggttc cgccggatcg gggcctccgc cgggttgagc tcggcggccc
      421 ggcccagcgc gtcggccgct ccttgccagt ccgacagcgc cgccagcgca ttgccgagat
      481 tgttccacgc ccgcccgtcg gcgggccttg ccgcggcgat gacctcccag gcctccttgg
      541 cggcccgggc gtcgccggcg gcatcggcgg cctcggcccg cgcgtgccac agcgcgaggc
      601 ctgcgggggc tgcgctcggc tcgggccggg gcagtgccag cgcttcggcg gcccggcccg
      661 cgtcgaccag cgcgcgcgac aggatcagcc ggtaggcggc gttgcccggc tcggcctcga
      721 gcgcccggcg gagatgttcg accccgctgc ccgggtcgcc tcggcggcag tggatcaggc
      781 cgagcaaatg gtcgatcgcg ggcgaaggag cgccggttgc gaggccctcg gccaggcgct
      841 gcgcctggtc gaggtcgccg cgctggaacg ccgccagggc ttcggcgagt tgcgggtcgg
      901 ggctcaccgc cgccctatcc gggaccgccc gcgagcggtc aacgggcgag gcgaatccgg
      961 gctcagccgt tgacgacgac cccgccgttg ggatgcaagg tctggccggt catgtagctt
     1021 gcatcctcgc aggcgaggaa caggaaggac ggagcgacct cgttgggctg gccgggccgg
     1081 cccatcggca cactcttgcc gaactccttc atgtcctcgg gcggctggcc gcccatcgga
     1141 ttgagcggag tccagatcgg cccgggcgcg accgcgttga cccggatccc gtccttgacg
     1201 aggttctgcg acagcgagcg ggtgaaggcg gtgatcgcgc ccttggtcgc gctgtagtcg
     1261 agcaaatcgt ccgagccctt gtacatcgtc accgacgtgc agttgatgat cgccgatccc
     1321 ttcttcaggt gcggccgggc ggcttgggtc atgaagaaca tgccgaagat gttggtctgg
     1381 aacgtccgct tcagctggtc ctcggtgatg tcgcggatgt ccttgtcggg gtgctgctcg
     1441 ccggcgttgt tgaccaatat gtcgagccgg ccgagctgtt ggacggtctg ggcgacggcg
     1501 cgctcgcaga agctcttgtc gccgatgtcg ccgggaatcg cgatcgcctt gcggccctcc
     1561 ttctcgacga tcgccttggt cttgttcgcg tcgtcgtgct cgcacaggta gaggatggcg
     1621 acgtcggcgc cctcgcgcgc gaacaaagcc gcgaccgccc ggccgatgcc gctgtcggcc
     1681 ccggtgatca aggcgacctt gcccttcagg cgatccgagc ccttgtagcg cggctcccag
     1741 tcgggcttgg gctgcagctg gctctcgtga ccgggcagcg cgtcctcatg gatcatcggc
     1801 tcgacgatgt cggacatggc tcttgctcct tgcgacggga aaaccgccgt ccgctcgggc
     1861 cggttccaat ttgccgcagg cgcgagccgt ccctaccttg gcggctcgat ggcgagcgcg
     1921 gcgcaaatca cggaacgcag gagcggcagc ttccggaccc tgctgcgatt cctgccgatg
     1981 ctgtggccga agggcgaggg cgagctcaag gcccgggtgg tcgccgcggt attgctggtg
     2041 ctcgccggca aggcggcggt gctgctgatg ccgttcgcct acaaggcggt catcgaccgc
     2101 atgtcggccg gaaccgcggt gttcgcggtc gtcgccggac tggtcgtcgc ctacagcgtc
     2161 gcgcgcttcg ccggggtgct cgccgacaac ctccgcaacg ctctgttcga aaaggtcggc
     2221 cagcgcgcgg cgcgcaagct cgccgggcgg gtgttccgcc acgtccacga cctgtcgctg
     2281 cgattccacc tcgagcggcg caccggaagc ctcaccaaga tcgtcgagcg cggcaccaag
     2341 agcatcgaca tgatgctcta tttcctgctg ttcaacatcg ccccgacggt catcgagctg
     2401 gccgcgatct gcgtgatctt cttcgtcaag ttcgggcccg ggctggtcgc cgcgaccctg
     2461 gcgatggtcg ccgtctacat cgtcttcacc cggaaggtga ccgactggcg ggcgcgcgtc
     2521 cagcgcgaga tgaacgaggt cgacaaccgt gcgatcggcc gcgcggtcga ctcgctcctg
     2581 aactatgaga cagtcaaata tttcggcgcc gaggagcgcg aggcgcggcg ctacgacgag
     2641 gcgatcggcg cctacgccga cgccgcgacc aagaatgaga cgtcactcgc ctggctcaac
     2701 atcggccagt cgctgatcac caatctgatg atggccgggg cgatgatctt caccgtctgg
     2761 ggctggagcc agggccgctt cacaccgggc gatgtcgtct tcatcaacac catgctgctg
     2821 cagctgttca ggccgctcga catgctgggc tgggtctatc gatcgatccg gcaaggactg
     2881 atcgacatgg aggcgat
//
LOCUS       DASTZO010000004         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_156928,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000004 DASTZO010000000
VERSION     DASTZO010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156928"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1550)
                     /gene="gatA"
                     /locus_tag="VFU20_00055"
     CDS             complement(72..1550)
                     /gene="gatA"
                     /locus_tag="VFU20_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU5284884.1"
                     /translation="MNALTSLTIAELRDGFRSGEFSAREVAEAFNAAVAGARALNAFT
                     VETPDDALAAADAADAARGKGDAGPLAGIPLGIKDLFATQGVDTTAGSRILKGFRPPY
                     ESTVSANLRKAGAGMLGKLNMDEFAMGSSNETSAYGPVISPWKRLDGGNAALTPGGSS
                     GGSAAAVAAGLAPGVTGTDTGGSIRQPAAFTGICGIKPTYGRCSRWGIVAFASSLDQA
                     GPMAKTVRDCAILLEAMAGFDPRDSTSLDLPVPRWEANLSSDLKGKRVGIPAEYRIDG
                     VPADIDALWDQGVEWLRDAGAEPVKISLPHTKYALPTYYIIAPAEASSNLARYDGVRY
                     GLREMGEGGLDGMYAATRAAGFGAEVKRRIMIGTYVLSAGFYDAYFTKAQKVRTLIKR
                     DFANAFESCDLILTPTAPSAAFALGDKSADPIAMYLNDVFAVPASLAGLPAMSVPGGL
                     DADGLPLGLHLIGRELDEQGVLNAGLAIEERAGFNHRPTRWW"
     gene            complement(1547..1834)
                     /gene="gatC"
                     /locus_tag="VFU20_00060"
     CDS             complement(1547..1834)
                     /gene="gatC"
                     /locus_tag="VFU20_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU5284885.1"
                     /translation="MSVTPEQVRHIARLARIAMSDAEIAALAPELNNILGWVEQVGEV
                     DTEGVEPLTAVIDQKLRLRDDVVTEGDCREAVLANAPAAEHGFFAVPKVIE"
     gene            1914..>2392
                     /locus_tag="VFU20_00065"
     CDS             1914..>2392
                     /locus_tag="VFU20_00065"
                     /inference="COORDINATES: protein motif:HMM:NF022732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEU5284886.1"
                     /translation="MCARRFLILVVILTLLAVAGAFAIFQFGNRVLLKSTVPRGEFVA
                     PPPSSGPDYAEGSSWIARPGLAGDPSGWRPAGEPRPQVPTAAHAFYIHPTTYLERDRW
                     NALLDDRASRDRAALFVQSQASAFADAAAVWAPRYRQAAYGAFLLDNEDARAALGLA"
BASE COUNT          341 a          865 c          820 g          366 t
ORIGIN      
        1 ctgggcgtga acctcgaggc cgaccaccac ttcccaatcg cccgtttcgc ccttgatgcg
       61 ataatcagtc attaccacca cctcgtcgga cgatgattga aaccggcccg ttcctcgatg
      121 gcgagacccg cgttgagcac gccctgctcg tcgagctcgc ggccgatcag gtgcaggccc
      181 aagggaaggc cgtccgcgtc cagccctccc ggaaccgaca tcgccggcag cccggcgagg
      241 ctcgccggca ccgcgaacac gtcgttcaaa tacatggcga tcgggtcggc gctcttgtcg
      301 ccgagcgcga acgccgccga cggggcggtc ggggtcagga tcaggtcgca gctctcgaac
      361 gcgttcgcga agtcgcgctt gatcagcgtg cgcaccttct gcgccttggt gaaataggcg
      421 tcgtagaagc cggccgagag cacataagtg ccgatcatga tgcggcgctt gacctcggcc
      481 ccgaaccccg cggcgcgggt cgcggcgtac atgccgtcca agccgccctc gcccatctcg
      541 cgcaggccgt agcggacgcc gtcgtagcgg gcgaggttcg acgaggcctc ggcgggcgcg
      601 atgatgtaat aggtcggcag cgcatatttg gtgtgcggga gcgaaatttt gaccggctcg
      661 gcgccggcgt cgcgcagcca ttcgacgccc tggtcccaca gggcatcgat gtcggcgggg
      721 actccgtcga tgcgatattc cgcgggaatt ccgacgcgct tgcccttgag gtcgctcgac
      781 aaattcgctt cccaccgcgg caccggcagg tcgagcgacg tcgagtccct gggatcgaaa
      841 ccggccatcg cctcgagcag gatcgcgcag tcgcggaccg ttttcgccat cggcccggcc
      901 tggtcgagcg aggaggcgaa ggcgacgatc ccccagcgcg agcaacggcc gtaggtcggc
      961 ttgattccgc aaatgccggt gaacgccgcc ggctggcgga tcgagccgcc ggtgtcggtg
     1021 ccggtgaccc ccggcgcgag ccccgccgcg accgccgccg ccgagccgcc cgacgagccg
     1081 cccggggtca gcgcggcgtt gcccccgtcc aggcgcttcc acgggctgat caccggcccg
     1141 taggcgctgg tttcgttcga cgagcccatc gcgaactcgt ccatgttcaa tttgcccagc
     1201 atccccgctc cggccttgcg caggttggcg ctgacggtcg attcgtacgg cggccggaag
     1261 cccttgagga tccggctgcc agcggtcgtg tcgacgccct gtgtcgcgaa cagatccttg
     1321 atcccgagcg ggattccggc gagcggcccg gcgtccccct tgccgcgcgc cgcatcggcc
     1381 gcgtcggccg cggcgagcgc gtcgtcgggc gtctcgacgg tgaacgcatt caaggcccgc
     1441 gccccggcga ccgcggcgtt gaacgcctcg gccacctcgc gggcgctgaa ctcgcccgaa
     1501 cggaagccgt cgcgaagctc ggcgatggtc agggaagtaa gcgcgttcac tcgatcactt
     1561 tcggcacggc gaagaagccg tgctcggccg ccggcgcgtt ggcgagcacc gcctcgcggc
     1621 aatcgccttc agtgacgacg tcgtcgcgca gccgcagctt ctggtcgatc accgcggtca
     1681 gcggctcgac gccctcggtg tcgacctcgc ccacttgctc gacccagccg aggatgttgt
     1741 tcaactcggg cgccagcgcg gcgatctccg cgtcgctcat cgcgatgcgc gcgagccgcg
     1801 cgatgtggcg cacctgttcg ggagtgacgg acatggaggc gcggctagca ggggcgggtt
     1861 aggccttcaa ccgccttgcc gcggcgcggc cgcgccaata gggccgggcg ccaatgtgcg
     1921 cgcgccgctt cctgatcctg gtcgtcatcc tgaccctgct cgcggtcgcg ggagcgttcg
     1981 cgatcttcca gttcggcaac cgagtgctcc tgaagagcac ggtgcccagg ggcgagttcg
     2041 tcgctccgcc gccgagcagc ggccccgact atgccgaggg ctccagctgg atcgcccggc
     2101 ccgggctggc cggcgacccg tccgggtgga ggccggccgg cgagccgcgg ccgcaggtcc
     2161 cgaccgccgc gcatgccttc tacatccacc cgacgaccta tctggagcgc gaccgctgga
     2221 acgccctgct cgacgaccgc gccagccgcg accgcgcggc cctgttcgtc cagagccagg
     2281 cgagcgcgtt cgccgacgcc gccgcggtgt gggcgccgcg ctaccgccag gcggcctacg
     2341 gggccttcct gctcgacaat gaggacgcgc gggcggcgct cggcctcgct ga
//
LOCUS       DASTZO010000005         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_757576,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000005 DASTZO010000000
VERSION     DASTZO010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757576"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VFU20_00070"
     CDS             complement(<1..656)
                     /locus_tag="VFU20_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU5284887.1"
                     /translation="MAARPTGTSTRRVRIAALILGFVGLALAASSVRASAQAAQPQSA
                     WAYRETPTAALDRHIRVLATSPRDFLALIGAGRAALELGDTHAAVGFFGRADEVFPSS
                     PLPQAGMGAAMVSDGDAYGAMRYFDRARQLGATAAMIGADRGLAYDLMGRHSDAQADY
                     RAALVGRDADEARRRLALSLAITGDKASAIETLAPLAARGDMGATRARALVLALSGDL
                     "
     gene            complement(764..2146)
                     /gene="ftsZ"
                     /locus_tag="VFU20_00075"
     CDS             complement(764..2146)
                     /gene="ftsZ"
                     /locus_tag="VFU20_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU5284888.1"
                     /translation="MTIEFMRPEVDELKPRISVIGVGGAGGNAIANMMRADVQGVDFI
                     VANTDAQALNTSVADRRIQLGLKITQGLGAGSRPEIGRAAAEETLDEIERALEGTHMC
                     FIAAGMGGGTGTGAAPVIAKTARDKGILTVGVVTKPFAFEGARRGRAADAGIDELQQH
                     VDTLIVIPNQNLFRLANSDTTFKEAFEMADEVLQQGVRGITDLMVMPGLINLDFADVR
                     SVMGEMGKAMMGTGEAIGDNRAIEAAEKAISNPLLDGVSMKGAKGVIISITGGEDMRL
                     MEVDEAASHIKELVDPDANIIWGSAFNNELGGKLRVSVVATGIEAETVAQAQPGKVFT
                     FPASRTTSAPAAPELPIAAIDEDSIPLDLAGDDDGDELLLDPEDILTSPVSGAPIAPP
                     ADEDLGATEDSAGDSEAPKAASESATLFERMSNIARGAAKAQGEDEEPTDYRREPIDI
                     PRFLNRQNNQ"
     gene            complement(2234..2287)
                     /locus_tag="VFU20_00080"
                     /pseudo
     CDS             complement(2234..2287)
                     /locus_tag="VFU20_00080"
                     /inference="COORDINATES: protein motif:HMM:NF012320.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          304 a          810 c          824 g          365 t
ORIGIN      
        1 tcgaggtcgc cgctgagcgc cagcaccagc gcccgggcgc gagtcgcgcc catgtcgccg
       61 cgggccgcga gcggcgccag ggtctcgatc gcgctggcct tgtcgccggt gatcgccagg
      121 ctcagcgcca gccgccgccg cgcctcgtcg gcatcgcgcc cgaccagcgc cgcgcgatag
      181 tcggcctggg cgtccgaatg gcggcccatc agatcgtagg cgaggccgcg gtcggcgccg
      241 atcatcgccg cggtcgcgcc gagctgccgc gcgcggtcga aatagcgcat cgccccgtag
      301 gcgtcgccgt cggagaccat cgccgcgccc atccccgcct gcggcagcgg gctcgacggg
      361 aagacctcgt cggctcggcc gaaaaagccg acggcggcgt gggtgtcgcc gagctcgagc
      421 gccgcccggc cggcgccgat cagcgccagg aagtccctgg gcgacgtcgc cagcacccgg
      481 atatggcggt cgagggccgc ggtcggagtc tcgcgatagg cccaggccga ttgcggctgg
      541 gcggcctgcg cggaggcgcg caccgacgag gccgccagcg cgagcccgac gaatcccagg
      601 atcagggcgg cgattcgaac gcggcgggtc gaggtgccag tgggtcttgc ggccatcgcc
      661 aatgccatgt ccgagcggag cttgcgcaaa gctttctggc cagcgcccga gcgacgagcc
      721 gtgcaacccc ggcgatgagg cggggctgcg gcagggtgaa gcgctactgg ttgttctgcc
      781 ggttgaggaa gcgcgggatg tcgatcggct cgcgccgata gtcggtcggc tcctcgtcct
      841 cgccctgcgc cttggcggcg ccgcgggcga tgttcgacat gcgctcgaac agggtggcgc
      901 tctcggacgc cgccttcggc gcctcggagt cgccggcgct atcctcggtc gcgcccagat
      961 cctcgtcggc cggcggagcg atcggagcgc ccgacaccgg gctggtcaga atgtcctcgg
     1021 ggtcgagcag cagctcgtcg ccgtcatcgt cgcccgcaag gtcgagcgga atgctgtcct
     1081 cgtcgatcgc cgcgatcggc agctcgggcg ccgcaggggc ggaggtcgtg cgtgacgccg
     1141 ggaaggtgaa caccttgccg ggctgcgcct gggcgacggt ctcggcctcg atcccggtcg
     1201 cgaccaccga cacgcgcagc ttgccgccca gctcgttgtt gaacgccgag ccccagatga
     1261 tgttggcgtc ggggtcgacc agctccttga tgtggctcgc cgcctcgtcg acctccatca
     1321 gccgcatgtc ctcgccgccg gtgatcgaga tgatcacccc cttggcgccc ttcatgctga
     1381 ccccgtcgag cagcgggttg gagatcgcct tctcggcggc ctcgatggcg cggttgtcgc
     1441 cgatcgcctc gccggtgccc atcatcgcct tgcccatctc gcccatcacc gagcggacgt
     1501 cggcgaagtc gaggttgatc aggccgggca tgaccatcag gtcggtgatc ccgcggacgc
     1561 cctgctggag cacctcgtcg gccatttcga acgcttcctt gaaggtggtg tccgaattgg
     1621 cgaggcggaa caaattctgg ttggggatga cgatcaacgt gtcgacgtgc tgctggagct
     1681 cgtcgatgcc ggcatcggcg gcgcggccgc ggcgagcgcc ctcgaacgcg aacggcttgg
     1741 tgacgacgcc gacggtcagg atgcccttgt cgcgcgcggt cttggcgatc accggcgcgg
     1801 cgccggtgcc ggtgccgccg cccatcccgg cggcgatgaa gcacatgtgg gtgccttcca
     1861 atgcacgctc gatctcgtcc aatgtttctt cggcggcggc gcggccgatc tcgggccgcg
     1921 agccggcgcc gaggccctgg gtgatcttga gcccgagctg gatgcggcgg tccgcgaccg
     1981 aggtgttgag ggcctgggca tcggtgttgg cgacgatgaa gtcgacgccc tggacatcgg
     2041 cgcgcatcat gttggcgatc gcgttgccgc cggcgccgcc gacgccgatc acgctgatcc
     2101 gcggcttgag ctcgtccact tccggccgca tgaattcgat cgtcatcagg tcactccccg
     2161 aaatacgcgc tcgcgccgcg cccgtgcccg tgccgccgcc catgccggcg gcgatgaagc
     2221 acatgtgcac gccttcgagg caccgctcga tttcctcgag cgtctcttcg gctgcggcgc
     2281 gccccacttc gggacgcgag ccg
//
LOCUS       DASTZO010000006         3307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_444351,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000006 DASTZO010000000
VERSION     DASTZO010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444351"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1551
                     /gene="lnt"
                     /locus_tag="VFU20_00085"
     CDS             22..1551
                     /gene="lnt"
                     /locus_tag="VFU20_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEU5284889.1"
                     /translation="MVPALIAALAGALSAFAFEPVGWWPLMPVAFAILCELLIRAGSL
                     RRALLTGWLFGLGQFVVGLNWIATAFTYQSAMPAWLGWVAVVLLSLYLAIYPALAAGL
                     GWRLGAGRRLPLLLALTGGWAVTEWLRAGIFTGFSWNPAGVVLAGTPLIQSSALIGTY
                     GLSMLVVLAGGAIWLELHRKYLAGVAIVVLAALLWALPASRVPDTPIAVRPIRIVQPD
                     IGQQDKWRPGFSAIAAQRLARLSAPRHPEAGPRLLFWPEAAVTDPLEDARTGEHQALA
                     EFERTRAAALLAPGEYLLTGGLALHSSDGRRVDGAANSVFVLGPGGKPVGRYDKAHLV
                     PYGEYLPMRPLLSSIGLSRLAPGDVDFTAGPGPRTLALPGWGKVGFQICYEIIFSGHV
                     VDARDRPDFIFNPSNDAWFGRWGPPQHLAQARLRAAEEGLPVLRATPTGISAIIDARG
                     ALVAAQPWRTAGVIDGVLPPPALGLTPFARFGNWIPLLLGLMMIAAAIALPKSERYRR
                     T"
     gene            1613..2842
                     /gene="metK"
                     /locus_tag="VFU20_00090"
     CDS             1613..2842
                     /gene="metK"
                     /locus_tag="VFU20_00090"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU5284890.1"
                     /translation="MRSNYLFTSESVSEGHPDKVADQISDALVDYFIGKDPESRVACE
                     TLVTTQRIVVGGEIRCAPVYDEDRFQDTHGWAPGARDEIEGIVRQVVHRIGYEQAGFH
                     WQTASFDNYLHGQSPHIAQGVDAAGNKDEGAGDQGIMFGFACDETPELMPATLYYSHK
                     ILERLAHDRHTGAAPFLEPDAKSQVTLRFEDGKPAAATAIVVSTQHAPGYHEGEKEAE
                     LKAYVRKVVAETLPAELLSDDTVYHINPTGKFEIGGPDGDAGLTGRKIIVDTYGGAAP
                     HGGGAFSGKDPTKVDRSAAYMARYLAKNIVAAGLASRCTIQLAYAIGVSAPLSLYVDT
                     HGTGTVEDQQLEAAIARIPSLGGLTPRGIRTHLKLNKPIYEPTAAYGHFGRTPEGDLF
                     PWEKLDLVEDLKAALAA"
     gene            complement(2839..>3307)
                     /locus_tag="VFU20_00095"
     CDS             complement(2839..>3307)
                     /locus_tag="VFU20_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284891.1"
                     /translation="LAGAIGGVLATAPAVLLLHAFGSLEVEAQILGLSVLATIGAGWL
                     VMAAAGAAYARLFGRAANDRRGGWLFGMAFGFALWAAGAVMVLPVASGGKAPAGMAAL
                     GVFLSLIVWGAALGAVIPLVHRPLHKKIEAGARLPRVGPTAIATGRNRLRGKR"
BASE COUNT          467 a         1237 c         1130 g          473 t
ORIGIN      
        1 gcaatggcta gcgagtgagc gatggtgcct gccttgatcg ccgccctcgc cggggccttg
       61 tccgccttcg ccttcgagcc ggtcggctgg tggccgctga tgccggtcgc cttcgcgatc
      121 ctgtgcgagc tattgatccg cgccgggtcg ctgcggcggg cgcttctgac cggctggctg
      181 ttcgggctcg gccagttcgt cgtcggcctc aactggatcg cgaccgcctt cacctaccag
      241 tcggcgatgc cggcgtggct cggctgggtc gcggtggtgc tgctgtcgct ctacctcgcc
      301 atctacccgg cgctggcggc ggggctcggc tggcggctcg gcgccggccg ccggctgccg
      361 ctgctgctgg cgctgaccgg cggctgggcg gtgaccgaat ggctgcgcgc cgggatcttc
      421 accggtttct cgtggaaccc ggccggggtc gtgctcgccg gcactccgct gatccagtcg
      481 agcgccctga tcggcaccta cgggctgtcg atgctggtcg tcctggccgg gggagccatc
      541 tggctcgagc tgcaccgcaa atatctcgcc ggggtggcga tcgtcgtgct ggccgccctg
      601 ctgtgggcgc ttcccgcgtc gcgggtgccc gacaccccga tcgcggttcg gccgatccgg
      661 atcgtccagc cggacatcgg ccagcaggac aagtggcggc cgggattcag cgcgatcgcc
      721 gcgcagcgcc tcgcccggct gtcggctccg cgccaccccg aggccgggcc gcggctgctg
      781 ttctggcccg aggcggcggt caccgacccg ctcgaggacg cgcgcaccgg cgagcaccag
      841 gcgctcgccg agttcgagcg cacccgcgcc gcggcgctgc tggcaccggg cgaatatctg
      901 ctgaccggcg ggctcgcgct gcactcgtcg gacggccgcc gggtcgacgg cgccgccaac
      961 agcgtcttcg tgctcggacc gggcggcaag cccgtcggcc gctacgacaa ggcgcacctc
     1021 gtcccctacg gcgaatatct gccgatgcgg cctctgctgt cgtcgatcgg cctgtcgcgg
     1081 ctcgcgcccg gcgacgtcga cttcaccgcc ggcccggggc cgcgcacgct ggccctgccc
     1141 ggctggggca aggtcggctt ccagatctgc tacgagatca tcttttccgg ccatgtcgtc
     1201 gacgcgcgcg accggcccga tttcatcttc aacccgtcca acgacgcctg gttcggccgc
     1261 tggggcccgc cgcagcattt ggcccaggcg cggctgcgcg ccgccgagga agggctgccg
     1321 gtgctccggg cgaccccgac cgggatcagc gcgatcatcg acgcccgcgg ggcgctcgtc
     1381 gccgcccagc cgtggcgcac cgcgggcgtg atcgacggcg tgctgccgcc gccggcgctc
     1441 ggactgacgc ccttcgcccg gttcggaaac tggatcccgc tgctgcttgg cctgatgatg
     1501 atcgccgccg ccattgccct tcccaaaagc gagcgctatc ggcggacata aggatttcct
     1561 tatatcattg aatccggccc cgcggcccga tcttccagga ccatttcgaa ccatgcgcag
     1621 caattacctg ttcacgtccg aatccgtttc cgaaggccat cccgacaagg tcgccgacca
     1681 gatcagcgac gccctggtcg attatttcat cggcaaggac ccggaatcgc gggtcgcctg
     1741 cgagaccctg gtcaccaccc agcggatcgt cgtcggcggc gagatccgct gcgccccggt
     1801 ctatgacgag gaccgcttcc aggacacgca cggctgggcg ccgggcgcgc gcgacgagat
     1861 cgaggggatc gtccgccagg tcgtccaccg catcggctac gagcaggccg gcttccactg
     1921 gcagaccgcg agcttcgaca attatctgca cggccagtcg ccccacatcg cccagggagt
     1981 cgacgcggcg ggcaacaagg acgagggcgc cggcgaccag gggatcatgt tcggcttcgc
     2041 ctgcgacgag acccccgagc tgatgccggc gacgctctat tacagccaca agatcctcga
     2101 gcggctggcc cacgaccgcc acaccggcgc ggcgcccttc ctcgagcccg acgccaagag
     2161 ccaggtgacc ctgcgcttcg aggacggcaa gccggccgcg gcgaccgcga tcgtcgtctc
     2221 gacccagcac gcgcccggct accacgaggg cgagaaagag gcggagctca aggcctatgt
     2281 ccgcaaggtg gtcgccgaga ccctgcccgc cgagctgctc agcgacgaca ccgtctatca
     2341 catcaacccg accggcaagt tcgagatcgg cgggccggac ggcgacgccg ggctgaccgg
     2401 gcgcaagatc atcgtcgaca cctacggcgg cgcggccccg cacggcggcg gcgccttttc
     2461 cggcaaggac ccgaccaagg tcgaccgctc ggccgcctac atggcgcgct acctcgccaa
     2521 gaacatcgtc gcggcgggcc tggcgagccg ctgcacgatc cagctcgcct acgcgatcgg
     2581 ggtcagcgcg ccgctgtcgc tctacgtcga cacgcacggc accggcacgg tcgaggacca
     2641 gcagctcgaa gcggcgatcg cgcgcatccc gagcctcggc ggcctgaccc cgcgcggcat
     2701 ccgcacgcac cttaagctca acaagccgat ctacgagccg accgccgcct acggccattt
     2761 cggccgcacg cccgagggcg acctgttccc gtgggagaag ctcgacctgg tcgaggattt
     2821 gaaggcggcg ctggcggcct agcgcttgcc gcgcaaccgg ttgcggccgg tggcgatcgc
     2881 ggtcgggccg acccgcggca ggcgggcgcc ggcctcgatc ttcttgtgca gcgggcggtg
     2941 gaccagcggg atgaccgctc ccagcgcggc gccccagacg atcagcgaca ggaagacccc
     3001 gagcgcggcc attcccgccg gcgccttgcc gccgctggcg accggcagga ccatcaccgc
     3061 cccggcggcc cacaaggcga acccgaaggc cattccgaac agccagccgc cgcgccggtc
     3121 gttggcggcg cggccgaaca ggcgcgcata ggcggctccg gcggcggcca tcaccagcca
     3181 gcccgcgccg atcgtcgcca ggaccgacag gccgaggatt tgcgcctcga cctcgagcga
     3241 gccgaacgcg tgcagcagca ggaccgcggg cgcggtggcg agcactccgc cgatcgcccc
     3301 ggcgagc
//
LOCUS       DASTZO010000007        10735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_653179,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000007 DASTZO010000000
VERSION     DASTZO010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 10735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10735
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653179"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFU20_00100"
     CDS             <1..304
                     /locus_tag="VFU20_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5284892.1"
                     /translation="EFKAAGVRVLGISRDPMKSHEKFIGKYGLGVPLASDEDGRISDA
                     FGTWVQKSMYGRKYMGMERSTFLIGADGRVLKAWRKVKVPGHAAEALEAAKELAAA"
     gene            complement(305..1750)
                     /locus_tag="VFU20_00105"
     CDS             complement(305..1750)
                     /locus_tag="VFU20_00105"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5284893.1"
                     /translation="MRLLLAASILAFSTTAIAQAPPSIITQPSKLDSKWQARTREIYE
                     TSVEIPTVAGRGQMQKQADYLASQLNAAGFAAEDIRILPYEGSEPNDKTVALIARWRA
                     DRPTAKPILLMAHMDVVEARREDWKQDPFEFIERDGYFYGRGSYDNKAGMVGLVASFL
                     KLKAAGWKPRRDLILFFTGDEETGGRGAALGATEWRPLTDAEYALNSDGGGGSFLASG
                     MPLGFGLQTAEKTYQSFTFTVRNKGGHSSRPRQDNAIYELATALKKVEAHRFQPMLNE
                     TTRAYFTARAAQEGDSALGKAIRAWLANEQDGAAADAIEANELEVGLTRTRCVATELQ
                     AGHADNALPQMARATVNCRIMPGISPDAVESELEGIIGKGVEVDKSRSYVGRPTPVSP
                     LRKDIVTAYQNAVRALNGPTVPVIPLMSTGATDGSFFREKGMPVYGIDGSWVISPEDE
                     RAHGLDERMPVRAVYDNVLFWEMVLKELAGR"
     gene            complement(1780..2883)
                     /gene="zapE"
                     /locus_tag="VFU20_00110"
     CDS             complement(1780..2883)
                     /gene="zapE"
                     /locus_tag="VFU20_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HEU5284894.1"
                     /translation="MTGSVGKAYAALVAVGELKPDPAQARAVAALDRLAQSSRRNGLF
                     DRLLGNRSRACDGVYLWGGVGRGKSMLMDLAFDHIAVEPKRRVHFHAFMLETHRRLRD
                     ARRSEEVDPLDEVAGRISAEARLLAFDEMVVNNPADAMILSRLFDKLIERGVKVVTTS
                     NRPPRDLYKDGLNRELFVPFIELIEQAMEVVAVDGPTDYRLDRLAGVAVWHVPNGPEA
                     TQALSTAFFKLTDHPVEDRAHVPSEELDVGGGRTLHVPKSLKGVAVFSFKRLCGEPRG
                     AADYLAIAQRFHTVILVGVPVMTKAMRNEAARFVTLIDALYEHRVKLLAAADAQPAGL
                     YPQGDGSFEFARTVSRLEEMRSAEYLAEGHGAA"
     gene            complement(2880..3392)
                     /locus_tag="VFU20_00115"
     CDS             complement(2880..3392)
                     /locus_tag="VFU20_00115"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF025008.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU5284895.1"
                     /translation="MSDAPSDPDDPDEGLMEDVGLPRGATPDPNHPGWFSWGDFPRGS
                     FAAATGRLLFKPDGPGRGICRMVPTEDHMNMGGSIHGGAVMSFIDMSMFAGGLCAGMA
                     RGHYVTLDLTTHFLARGKAGPPLEAHVELVRQTPGHAFLQGVVRQDGEPCYSFTGTLK
                     RVNTPNRPAA"
     gene            complement(3529..4308)
                     /locus_tag="VFU20_00120"
     CDS             complement(3529..4308)
                     /locus_tag="VFU20_00120"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HEU5284896.1"
                     /translation="MAEFRLPKNSRVTRGLAHPPESGRRLKSFKIYRYDPDSGANPRF
                     DTYTIDLDKCGPMVLDALIKIKNEIDPTLTFRRSCREGICGSCSMNMDGRNGLACTTP
                     IADIKGEVRITPLPHMDVVKDLVPDLTHAYAQYALIQPWLKAATPAPSGKERLQSPDD
                     RARLDGLYDCILCFCCSTSCPSYWWNQDRFLGPAVLLQAYRWLADSRDEMTGERLDQL
                     EDPFRLYRCHTIMNCANVCPKGLNPAKAIAETKKLIAERAA"
     gene            complement(4418..4822)
                     /locus_tag="VFU20_00125"
     CDS             complement(4418..4822)
                     /locus_tag="VFU20_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284897.1"
                     /translation="MKTLILLAGAALLASAGPALAKPNHAKHNKGQHARVLHDHRGHV
                     GYGTGGCPPGLAKQNAECMPPGQHKKLFAVGQRVPSGYRGLLGYDALPYDLRSRYGTG
                     LDPYSRYIYRDDYLYRVDPRTMVVSEILRAIL"
     gene            complement(4922..5500)
                     /locus_tag="VFU20_00130"
     CDS             complement(4922..5500)
                     /locus_tag="VFU20_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284898.1"
                     /translation="MKKLLLLAGAGMMLALGAPAAADAKPGKGGAKVHMNAKGHIDMN
                     RNGLADWREQRLIDANGNGILDWREQRRVDINRNGVPDWRERWIDRDRDGIDDRQEIM
                     ANRWGGATCPPGLAKKIPPCVPPGQVGRTFTVGQRVPTGWGTLPYGRIPVGVRDLYDL
                     DDDDRYIYRNGTLYVVDPRTRLIEQIIAGLVF"
     gene            complement(5674..6159)
                     /locus_tag="VFU20_00135"
     CDS             complement(5674..6159)
                     /locus_tag="VFU20_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284899.1"
                     /translation="MTLEEWAFVAQIVSAIAVVASLVFVGFQLRHATAAIRATSSQAH
                     SSLYTDLVQSIIDNADFARVWSIGINEPKKLSVEEWVRFVAYASALFRLYESSRVQWL
                     NGRLDEEHWHTIERQAADFGHLPGLQGAWELRSHWYSPEFRAWFDGLKPPEPPRPYAR
                     D"
     gene            6325..7287
                     /gene="mdh"
                     /locus_tag="VFU20_00140"
     CDS             6325..7287
                     /gene="mdh"
                     /locus_tag="VFU20_00140"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU5284900.1"
                     /translation="MARRKIALIGAGMIGGTLAHLAAMRELGDIILFDIAEGIPEGKA
                     LDIAQAGPVDDFDARLKGTSDYADIAGADVCIVTAGVPRKPGMSRDDLLGINLKVMKA
                     VGEGIKKHAPGSFVICITNPLDAMVWALREFSGLPHQRVVGMAGVLDSARFRHFLAEE
                     FQVSVEDVTAFVLGGHGDTMVPIVEYSTVAGIPIPDLVKMGWSTKERIDAIVQRTRGG
                     GGEIVALLKTGSAYYAPATAAIAMTESFLKDKKRVLPAAAHLTGQYGVDDLYVGVPVV
                     IGGEGVERIVEIELDEAAKANFQVSVDAVKELLVACRQIDGSLK"
     gene            7284..8168
                     /gene="sucD"
                     /locus_tag="VFU20_00145"
     CDS             7284..8168
                     /gene="sucD"
                     /locus_tag="VFU20_00145"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU5284901.1"
                     /translation="MSILVDRNTKVITQGMTGETGTFHTQQALAYGTQMVGGVTPGKG
                     GTTHLGLPVFDTVREAVEATGANASVVYVPPPFAADSILEAIDAEVPLIVAITEGIPV
                     LDMVKVKRALSGSKSRLIGPNCPGVLTPDECKIGIMPGNIFSKGSVGVVSRSGTLTYE
                     AVFQTTNEGLGQSTAVGIGGDPVNGTNFVDVLEMFLADDETQSIVMIGEIGGSAEEDA
                     AQFLADEAKRGRSKPVVGFIAGRTAPPGRRMGHAGAIVSGGKGGAEDKIAAMEAAGIT
                     VSPSPSELGRTLKQLLES"
     gene            8182..>10735
                     /locus_tag="VFU20_00150"
     CDS             8182..>10735
                     /locus_tag="VFU20_00150"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEU5284902.1"
                     /translation="MDAVSHERDAGPSWARPHWPLAELDELNLGLDPTQAAIEKFAAA
                     VRDKASAAGADPEAVRRAAEDSIRAMMLIRTYRVRGHLAANLDPLGLHKSEPPADLTP
                     AYHGFSDADLDRPVHICGALGFEQASVRQIVERLRASYCGTIGIEYMHINDLDERRFL
                     QERIEADRPIEFTREGKQAILAKVIEAEEWEKFLARKYVGTKRFGLDGGESAVPGLEA
                     VIKYGGQYGVDDVAIGMAHRGRLNVLSNVMGKPYRAIFHEFAGGATNPADVGGSGDVK
                     YHLGTSSDREFDGNKVHLSLLPNPSHLEAVDPVVLGKARAVMTAKGDKHGKTVLPILL
                     HGDAAFAGQGVVAECLGFSGLPGYGTGGTIHFIINNQVGFTTSPQFARSSPYPSDIAK
                     SIQAPILHVNADDPEAVTFCCKLAIEFRHSFGRDIVIDMWCYRRFGHNEGDEPSFTQP
                     LMYAAIRKHPPISQVYGRRLIEEGVVDAQWIERHTRAYVDHLEEEFKAATSYLPNKAD
                     WFEGRWAGLGRPDEPVLGRRNTTTAIGEDSVARLGEALTRVPGNFHIHKTLQRILDAK
                     KAMFDSGSGFDWATAESMAFGSLILEGHNVRLSGQDSGRGTFSQRHAVWVDQSSGEKY
                     IPLCSVEGGRFEVRDSPLSEFGVLGFEYGYSIADPRTLVLWEAQFGDFANGAQTIVDQ
                     FIGSGEAKWLRASGLVMLLPHGFEGQGPEHSSARLERYLQLCAEDNLQVANCTTPANY
                     FHILRRQLLRDFRKPLILMTPKSLLRHKLAMSDIGDFTGDSHFRRILSDLNGPGEGET
                     KRLVLCSGKLAYELMEARDEAGDLGVEIIRIEQLYPFPSEPLIKRLKAAPNLQ"
BASE COUNT         1731 a         3567 c         3699 g         1738 t
ORIGIN      
        1 cgagttcaag gcggccggag tgcgggtgct cgggatctcg cgcgacccga tgaagagcca
       61 cgagaagttc atcggcaaat acggcctcgg agtcccgctc gcctcggacg aggacgggcg
      121 gatttccgac gcgttcggaa cctgggtcca aaagtcgatg tacgggcgca aatatatggg
      181 gatggagcgc tcgaccttcc tgatcggcgc cgacggccgg gtgctcaagg catggcgcaa
      241 ggtcaaggtg ccgggccacg ccgccgaggc gctggaggcg gcgaaggagc tggcggccgc
      301 ctgactatct gccggcaagc tccttcagca ccatttccca gaacagcacg ttgtcgtaga
      361 cggcgcggac cggcatccgt tcgtcgaggc cgtgggcgcg ctcgtcctcc ggggagatca
      421 cccagctgcc gtcgatgccg tagaccggca tgcccttctc gcggaagaac gacccgtcgg
      481 tcgccccggt cgacatcaaa ggaatgaccg gcacggttgg gccgttcaac gcccgcactg
      541 cgttctggta cgccgttaca atgtccttgc gcagcggcga gaccggcgtc ggccggccga
      601 catagctgcg ggacttgtcg acctcgacac ccttgccgat gatcccctcc agctccgact
      661 cgaccgcatc cgggctgatt ccgggcatga tccggcaatt gaccgtcgcc cgcgccatct
      721 gcgggagcgc attgtcggcg tgtccggcct gcaactccgt ggcgacgcag cgggtgcgcg
      781 tcaggccgac ttcgagctca ttggcctcga tcgcgtcggc ggcggcccca tcctgctcgt
      841 tggcgagcca ggcgcgaatc gccttgccga gcgcgctgtc gccttcctgc gccgctcgcg
      901 ccgtgaaata ggcccgcgtc gtctcgttca gcatcggctg gaaacggtgc gcctccacct
      961 tcttcaacgc ggtcgccagc tcgtaaatgg cattgtcctg gcgcgggcgg ctggaatggc
     1021 cgcccttgtt tcggaccgtg aaagtgaagc tctggtaggt cttttcggcg gtctgcaggc
     1081 caaagccgag cggcattccg gatgcgagga aggagccgcc gccaccgtcg ctgttgagcg
     1141 catattcggc atcggtcagc ggccgccatt cggtcgctcc aagcgccgct ccccgcccgc
     1201 cggtttcctc gtcgccggtg aagaagagga tgaggtcgcg ccttggcttc cagccggctg
     1261 ccttgagctt caggaaggag gcgaccaggc cgaccatgcc ggccttgttg tcgtagctgc
     1321 cgcggccgta gaaatagccg tcgcgctcga tgaactcgaa cgggtcctgc ttccaatcct
     1381 cgcgcctggc ttcgaccacg tccatgtgcg ccatcaacag gatcggcttc gcggtgggcc
     1441 ggtcggcgcg ccaccgcgcg atcagggcga cggtcttgtc gttcggctcg ctgccctcgt
     1501 agggaaggat ccggatgtcc tcggccgcga acccggccgc attcaactgg ctcgccagat
     1561 aatcggcctg cttctgcatc tggccgcggc cggcaaccgt cgggatctcg accgaggttt
     1621 catagatttc acgcgtcctg gcctgccatt tggagtcgag ctttgatggt tgggtgatga
     1681 tgctgggcgg ggcctgggcg atggccgtgg tcgagaaagc cagaatcgaa gcggcaagca
     1741 gcaggcgcat gggcatccct cccggaattt ccgcgaacgc taagcggcgc cgtggccctc
     1801 ggcaagatat tcggcgctgc gcatttcctc gaggcggctg acggtgcgcg cgaactcgaa
     1861 gctgccgtcg ccttgcgggt aaagcccggc cggctgcgcg tcggcggcgg cgagcagctt
     1921 gacgcgatgc tcgtagagcg cgtcgatcag cgtcacgaag cgcgccgcct cgttgcgcat
     1981 cgccttggtc atcacgggca cgccgaccag gatcacggtg tggaagcgct gggcgatggc
     2041 gagatagtcg gcggctccgc gcggctcgcc gcacagccgc ttgaacgaga acaccgcgac
     2101 ccccttgagg ctcttgggga cgtgcagcgt ccggccgccg ccgacgtcga gctcttcgga
     2161 cggcacatgc gcccggtcct cgaccggatg gtcggtgagc ttgaaaaagg cggtgctcag
     2221 cgcctgcgtc gcctcgggcc cgttgggcac gtgccacacc gcgacacccg ccaggcggtc
     2281 gagccggtag tcggtcggcc cgtccaccgc gaccacctcc atggcttgct cgatcagttc
     2341 gatgaagggc acgaacagct cgcggttgag gccgtccttg tagaggtcgc gcggcgggcg
     2401 gttggaggta gtgacgacct tcactccgcg ctcgatcagc ttgtcgaaca atcgcgacag
     2461 gatcatcgcg tcggccgggt tgttgacgac catctcgtcg aacgccagca gccgcgcctc
     2521 ggcggagatg cgcccggcga cctcgtcgag cgggtcgact tcctcgctcc tgcgcgcgtc
     2581 gcgcagccgg cgatgggtct cgagcatgaa ggcgtggaag tggacccggc gcttcggctc
     2641 gacggcgata tggtcgaagg cgaggtccat cagcatcgac ttgccgcgcc cgaccccgcc
     2701 ccacaggtag acgccgtcac aggcgcggct gcgattgccg aggagccgat cgaagagccc
     2761 gttgcgcctg ctcgattgcg cgagccggtc gagcgccgcg accgcgcgcg cctgcgccgg
     2821 gtcgggcttg agctcgccca ccgcgaccag cgcggcataa gccttgccga ccgaaccggt
     2881 catgccgcgg ggcggttggg cgtgttcact cgcttgaggg tgccggtgaa gctgtagcag
     2941 ggctccccgt cctgccggac gacgccttgc aggaaggcgt ggccgggggt ctggcgaacc
     3001 agctcgacgt gcgcctcgag cggcggtccg gccttgccgc gggcgaggaa atgggtggtg
     3061 aggtcgagcg tcacataatg gccgcgcgcc atccccgcgc acaggccgcc ggcgaacatg
     3121 ctcatgtcga tgaagctcat caccgccccg ccgtggatcg agccgcccat gttcatatgg
     3181 tcctcggtcg ggaccatccg gcagatgccc cggccggggc catcgggctt gaacagcagc
     3241 cggccggtgg cggccgcgaa cgagccgcgc gggaaatcgc cccagctgaa ccagccggga
     3301 tggttgggat cgggcgtggc gccgcgcggc aggccgacgt cctccatcag cccttcgtcc
     3361 ggatcatctg gatcgctggg ggcgtcgctc acgctgcgcg gtgtctttca gggccgcggt
     3421 ccgtcaagcg gcccgccttt tgagtgggtc gcggcccgtc aagcggccct ccactttaat
     3481 gagtcgcggc ccgtcaagcg gccctccact ttaatgggtc gcggcccgtc aagcggcccg
     3541 ctccgcgatc agtttcttgg tctcggcgat cgccttggcc gggttgaggc ccttcgggca
     3601 gacgttcgcg caattcatga tcgtgtggca gcggtagagc cggaacgggt cctcgagctg
     3661 gtcgaggcgc tcgccggtca tctcgtcgcg gctgtcggcc agccagcggt acgcctgcag
     3721 gagcactgcg gggccgagga agcggtcctg gttccaccag tagctcgggc agctggtcga
     3781 gcagcagaag cacaggatgc agtcgtagag cccgtcgagc ctcgcgcggt cgtcgggcga
     3841 ttgcagccgc tccttgcccg acggggccgg agtcgccgcc ttcagccacg gctggatcag
     3901 cgcatattgc gcataggcat gggtgaggtc gggcaccagg tccttgacca cgtccatgtg
     3961 cggcaggggg gtgatgcgga cctcgccctt gatgtcggcg atcggcgtcg tgcaggcgag
     4021 gccgttgcgg ccgtccatgt tcatcgagca ggagccgcag atgccctcgc ggcacgagcg
     4081 ccggaaggtc agcgtcgggt cgatctcgtt cttgatcttg atcagcgcgt cgaggaccat
     4141 cggcccgcat ttgtcgaggt cgatggtgta ggtgtcgaag cgcgggttgg cgccgctgtc
     4201 ggggtcgtag cggtagatct tgaacgactt gagccgccgg ccgctctcgg gcggatgggc
     4261 gagccccctg gtgacgcggc tgttcttcgg cagtcgaaac tcggccaagg cacgctccta
     4321 tgctcgccga gccgctagca tggggaaagg cgcaaagaaa agggccgcgc ggccagggat
     4381 cccgcgcggc ccaaaccggc ccgatcgatg gcggttccta gaggatggcg cgcaggatct
     4441 cgctgaccac catcgtccgc gggtcgaccc ggtagagata gtcgtcgcgg tagatgtagc
     4501 gcgaataagg gtcgaggccg gtgccgtagc ggctgcgcag gtcgtagggc agggcgtcgt
     4561 agccgagcag cccgcgataa cccgaaggca cgcgctggcc gacggcgaac agcttcttgt
     4621 gctgtccggg cggcatgcac tcggcgttct gcttggcgag ccccggcggg cagccgccgg
     4681 tgccgtagcc gacatggccg cggtgatcgt gcagcacgcg cgcgtgctgg cccttgttgt
     4741 gcttggcatg gttcggcttg gcgagagccg gaccggcgga agcgagcagc gcagcgcccg
     4801 cgagcaggat gagtgttttc atggggccgg acgtagcggc tgggagatga acggctgttg
     4861 accgtgaaaa gggagccgcc cggtcgcccg gacggctcct gcgattgtct ggatcgagcg
     4921 cctagaagac caggccggcg atgatctgct cgatcagccg ggtgcgcggg tcgaccacgt
     4981 agagcgtgcc gttgcggtag atgtagcggt cgtcgtcatc gaggtcgtag aggtcgcgga
     5041 ccccgaccgg gatgcggccg tacgggagcg tcccccagcc ggtcggaacg cgctggccga
     5101 cggtgaaagt gcggccgacc tgccccggag ggacgcacgg cgggatcttc ttggccaggc
     5161 ccggcgggca ggtcgcgccg ccccagcggt tggccatgat ctcctggcgg tcgtcgatcc
     5221 cgtcgcggtc gcggtcgatc cagcgctcgc gccagtcggg gaccccgttg cggttgatgt
     5281 cgacccggcg ctgctcgcgc cagtcgagga tgccgttgcc gttggcgtcg atcagccgct
     5341 gctcgcgcca gtcggcgagc ccgttgcggt tcatgtcgat gtggcccttg gcgttcatgt
     5401 ggaccttggc gccgcccttg ccgggcttgg cgtcggcggc ggcgggcgcg ccgagcgcga
     5461 gcatcattcc ggcaccggcg agcagaagca gctttttcat gtccaatctc ctttcaaggc
     5521 agacggacgc gaagggcatg gtgctccttg cgtccggaca tgtgcttgaa acgattgccg
     5581 aagtggcccg ttcccggtcc gttcatgtgg gcgaaagctt gggcggttgc cgcgccgggg
     5641 ccgtgcatcg cgcgtcaagg ctgggggacc tgctcagtcg cgagcgtaag gccgcggcgg
     5701 ctcgggtggc ttgagcccgt cgaaccaggc gcgaaattcc ggcgagtacc agtggctgcg
     5761 cagctcccag gcgccctgaa gccccggcag atgaccgaag tcggccgcct ggcgctcgat
     5821 ggtgtgccaa tgctcctcgt cgaggcgccc attgagccac tgcacgcggg aggattcgta
     5881 cagccggaag agcgcgctcg cataagcgac gaagcgcacc cattcctcta cggacaattt
     5941 cttgggctcg ttgattccga tcgaccagac gcgcgcaaaa tcggcgttgt cgatgatgct
     6001 ctggaccaga tcggtgtaca ggctggagtg cgcctgggag gacgtggcgc ggatcgccgc
     6061 ggtcgcgtgc ctgagctgaa agccgacgaa caccagcgac gccacgacgg cgatcgcgga
     6121 aacgatctgg gcgacgaatg cccattcctc gagagtcatg gtccagcgct ccctcagccg
     6181 ggatcgcgcc ctatagcacg ggcagcggcg caagtggcgc gggcggttgc cgcgcccggg
     6241 ccgtgcggct aagcgggcgc cgcatttccc gatggccgcc tcgtccgggc cggccggaca
     6301 tgatgaaagg ttgaaccccg ccttatggct cgacgcaaaa tcgcgctgat cggcgccggg
     6361 atgatcggcg gcacgctcgc ccacctcgcc gcgatgcgcg agctcggcga catcatcctg
     6421 ttcgacatcg ccgagggcat ccccgagggc aaggcgctcg acatcgccca ggccgggccg
     6481 gtcgacgatt tcgacgcaag gctcaagggc acgtccgact atgccgacat cgccggcgcc
     6541 gacgtgtgca tcgtcaccgc cggtgtgccg cgcaagcccg gcatgagccg agacgacctg
     6601 ctcggcatca atttgaaggt catgaaggcg gtcggcgagg gcatcaaaaa gcacgcgccc
     6661 ggcagcttcg tcatctgcat caccaacccg ctcgacgcga tggtctgggc gctgcgcgag
     6721 ttctcgggac ttccccacca gcgcgtggtc ggcatggccg gcgtcctcga cagcgcccgc
     6781 ttccgccatt tcctcgccga ggagttccag gtcagcgtcg aggacgtcac cgccttcgtg
     6841 ctcggcggcc acggtgacac gatggtgccg atcgtcgaat attcgaccgt cgccgggatc
     6901 ccgatccccg acctggtcaa gatgggctgg tcgaccaagg agcgcatcga cgcaatcgtc
     6961 cagcgcacgc ggggcggcgg cggcgagatc gtcgctttgc tcaagaccgg gagcgcttat
     7021 tacgccccgg cgaccgccgc catcgcgatg accgagagct tcctgaagga caagaaaagg
     7081 gtgcttccgg ccgcggcgca cctcaccggc cagtacggcg tcgacgacct ttatgtcgga
     7141 gtgccggtgg tgatcggcgg ggaaggggtc gagcggatcg tcgaaatcga gctcgacgag
     7201 gccgccaagg ccaatttcca ggtcagcgtc gatgcggtca aggagctgct ggtggcgtgc
     7261 aggcagatcg acgggtcgct caagtgagca tcctcgtcga ccggaacacc aaggtgatca
     7321 ctcaggggat gaccggcgag accggcacct tccatacgca gcaggcgctc gcctacggca
     7381 cccagatggt cggaggagta acgccgggga agggcggcac gacccatctc ggcctgccgg
     7441 tgttcgacac ggtccgcgag gcggtcgagg cgaccggcgc gaacgcgagc gtcgtctacg
     7501 tgccgccgcc gttcgccgcc gactcgatcc tcgaggcgat cgacgccgag gtgccgctga
     7561 tcgtcgccat caccgagggc atcccggtgc tcgacatggt caaggtcaag cgcgcgctgt
     7621 cgggctccaa gtcgcggctg atcggtccga actgcccggg cgtgctgact cccgacgagt
     7681 gcaagatcgg gatcatgccc ggcaacatct tctccaaggg cagcgtcggg gtcgtgtcgc
     7741 ggtccggcac cctgacctac gaggcggtgt tccagaccac caacgagggg ctcggccagt
     7801 cgaccgcggt cggcatcggc ggcgacccgg tcaacgggac gaacttcgtc gacgtgctcg
     7861 agatgttcct cgccgacgac gagacccagt cgatcgtcat gatcggcgag atcggcggca
     7921 gcgccgagga agacgccgcg caattcctcg ccgacgaggc gaagcgcggg cgctccaagc
     7981 ccgtcgtcgg cttcattgcc ggccgcaccg ccccaccggg gcggcggatg ggccacgccg
     8041 gggcgatcgt ctcgggcggc aagggcggcg ccgaggacaa gatcgcggcg atggaggcgg
     8101 ctggaattac cgtttccccg agcccatctg agctcgggcg gacgctcaaa cagcttctcg
     8161 agagttaata ccaccaagcc catggacgca gtcagccacg aacgcgacgc cggcccgagc
     8221 tgggcgcggc cccattggcc tctcgccgag ctcgacgaat tgaacctcgg gctcgacccg
     8281 acgcaggcgg caatcgagaa attcgcggcg gcggtccgcg acaaggcgtc cgcggccggc
     8341 gccgacccgg aggcggtgcg gcgcgcggcc gaggattcga tccgcgcgat gatgctgatc
     8401 cgcacctatc gggtgcgcgg gcacctcgcc gccaacctcg acccgcttgg gctccacaag
     8461 agcgagccgc cggccgacct gacgcccgcc tatcacggct tttcggacgc cgacctcgac
     8521 cgcccggtcc atatctgcgg cgcgctcggc ttcgagcagg cgagcgtccg ccagatcgtc
     8581 gagcgcctgc gggccagcta ctgcggcacc atcggcatcg aatatatgca catcaacgac
     8641 ctcgacgagc gccgcttcct ccaggagcgc atcgaggccg accggccgat cgagttcacc
     8701 cgcgaaggca agcaggcgat cctcgccaag gtgatcgagg ccgaggagtg ggaaaagttc
     8761 ctcgcccgga aatatgtcgg caccaagcgc ttcgggctcg acggcggcga gagcgcggtc
     8821 cccggcctcg aggcggtgat caagtatggc ggccagtacg gggtcgacga cgttgccatc
     8881 ggcatggccc accgcgggcg attgaacgtg ctttcgaacg tgatgggcaa gccgtaccgg
     8941 gcgatcttcc acgagttcgc cggcggcgcg accaacccgg ccgacgtcgg cggctcgggc
     9001 gacgtcaaat accatctcgg cacctcgtcc gaccgcgagt tcgacggcaa caaggtgcat
     9061 ttgtcgctgc ttcccaaccc gtcgcatctg gaagcggtcg acccggtggt gctcggcaag
     9121 gcgcgcgcgg tgatgaccgc caagggcgac aagcacggta agacggtgct tccgatcctg
     9181 ctccacggcg acgccgcctt cgccggccag ggcgtggtcg ccgagtgcct cggcttttcc
     9241 ggactgcccg gctacggcac cggcggcacg atccatttca tcatcaacaa ccaggtcggc
     9301 ttcacgacct cgccgcagtt cgcgcgctcg tcgccctacc cttcggacat cgccaaatcg
     9361 atccaggcgc cgatcctcca cgtcaacgcc gacgatcccg aggccgtgac cttctgctgc
     9421 aagctggcga tcgagttccg ccacagcttc ggccgcgaca tcgtcatcga catgtggtgc
     9481 tatcgccggt tcggccacaa cgagggcgat gagccgagct tcacccagcc gctgatgtac
     9541 gcggcgatcc gcaagcaccc gccgatcagc caggtctacg gccgcaggct gatcgaagag
     9601 ggcgtcgtcg acgcccagtg gatcgagcgg cacaccaggg cctacgtcga ccacctcgag
     9661 gaggaattca aagcggcgac ctcctacctg ccaaacaagg ccgactggtt cgagggccgc
     9721 tgggctggac tcggccgccc cgacgagccg gtgctcggcc ggcgcaacac gacgacggcg
     9781 atcggcgagg attcggttgc gcggctgggc gaggccttga cccgggtccc cggcaatttc
     9841 cacatccaca agacgctgca gcgcatcctc gacgccaaga aagcgatgtt cgacagcggc
     9901 tccggcttcg actgggcgac cgccgagtcg atggcgttcg gaagcctgat cctcgagggc
     9961 cacaatgtcc ggctgtcggg gcaggacagc ggccgcggca ccttcagcca gcgccacgcg
    10021 gtgtgggtcg accagtcgag cggcgagaaa tacatcccgc tgtgctcggt cgaggggggc
    10081 cgcttcgaag tgcgcgattc gcccctgagc gagttcggag tgcttggctt cgaatatggc
    10141 tattcgatcg ccgacccgcg cacgctggtg ctgtgggaag cccagttcgg cgacttcgcc
    10201 aacggcgccc agaccatcgt cgaccagttc atcggctcgg gcgaggcgaa gtggctgcgc
    10261 gccagcgggc tggtgatgct gctcccgcac ggcttcgagg ggcaggggcc cgagcatagc
    10321 tcggcgcggc tcgagcgcta cctgcaattg tgcgccgagg acaatctcca ggtcgccaac
    10381 tgcacgaccc cggccaatta tttccacatc ctccgccgcc agctgctgcg cgatttccgc
    10441 aagcccttga tccttatgac gcccaagtcg ctgctccgcc acaagctcgc catgtccgac
    10501 atcggcgact tcaccggcga cagccacttc cgccgcatcc tgagcgacct caacgggccg
    10561 ggcgaaggcg agacgaagcg gctggtcctg tgctcgggca agctggccta cgaactgatg
    10621 gaagcgcgcg acgaggcagg cgacctcggc gtcgagatca tccgcatcga gcagctctac
    10681 ccgttccctt ccgagccgct catcaagcgg ctgaaggcgg cacccaatct ccagg
//
LOCUS       DASTZO010000008         3511 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_679408,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000008 DASTZO010000000
VERSION     DASTZO010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3511)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3511)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3511
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_679408"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2047
                     /gene="pepN"
                     /locus_tag="VFU20_00155"
     CDS             <1..2047
                     /gene="pepN"
                     /locus_tag="VFU20_00155"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEU5284903.1"
                     /translation="WHDPFPKPCYLFAMVAGDLVCNRDSFTTRSGREVSLGIWVRESD
                     LPKTAHAMDSLKAAMAWDERVYGREYDLDQFNIVAVSDFNMGAMENKGLNIFNSRYIL
                     ADKETATDSDFDAVAGVVGHEYFHNWSGNRVTCRDWFQLSLKEGFTVFRDQSFSADIG
                     SVAVKRIEDVRLLRAVQFPEDDGPLAHPVRPDSYLEISNFYTATVYNKGAELIRMFHT
                     LLGPEIFRAGTDLYFDRHDGQAATCDDFVQALEDASGVDLEPFKIWYAQAGTPKVTAR
                     LDHDSAARTATLRLAQRVEPTPGQPVKSPMPIPLRTALIGANSGGEVAPERLILLDQA
                     EQSIRFDEVAEPPLLSINRGFSAPVIIEAQRGPDELELLARHDRDPFARFEAMQELML
                     RALIAGTRGEPVDTAPLVRAASNTLRSEALDDAFKAEVVLPPSESQIGDRMDRVDPDA
                     IHASRERLRATLGKELRAELDAVRAIVPAADLSPAAKGARRLRSVALGLVAAADPAEG
                     AALAKAQFDAAANMTDRQGALAILVSLDRPERQEALDSFYRRFEDDPLVVDKWFALQA
                     TAQRADTIDEVERLAAHPAFTLANPNRLRALAGTFAANQWVFHHRSGRGYRFVADMIL
                     AADRLNPQVAARLVPPFGRWKRFDDDRAARMRSELERIVAAPGLSKDVYEQASKSLA"
     gene            2107..2565
                     /locus_tag="VFU20_00160"
     CDS             2107..2565
                     /locus_tag="VFU20_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5284904.1"
                     /translation="MQATIVKAGERAPLNVLGMPLRFLCDARETGGAWSLFEEEVPLG
                     MGPPPHRHDWDEAYYVLEGEVEFQIDGERVQSKAGDFNYLPRNTVHAFRGASSSPARV
                     LIFAAPAHASEFFEEVNSEVRSLPDDLAKIPEIGRRHGIQFLPPEAPRAG"
     gene            complement(2584..3186)
                     /locus_tag="VFU20_00165"
     CDS             complement(2584..3186)
                     /locus_tag="VFU20_00165"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HEU5284905.1"
                     /translation="MKLLLRTSLPVVLAAALGACSLGGLLGGGKTPPTLLKLTPQAAE
                     QGEMARSASAGEAVTISVPVVAKELRTVRVPAQVSHMEVAYIKDLQWVDTPDRLFQDL
                     LSETVRRTTGRVVLDPKQALLDPGVVVSGQLHKMGYDAAEQAVIVRFDGALSAAGGTR
                     VETRRFEAKVPASGDAASVGPALNQAANQVALEAAGWIGR"
     gene            complement(3186..>3511)
                     /locus_tag="VFU20_00170"
     CDS             complement(3186..>3511)
                     /locus_tag="VFU20_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MCE family protein"
                     /protein_id="HEU5284906.1"
                     /translation="IAAQRVGVLADSTNRLVVEEGRPAAQDLRRAIAGVERATSNLDA
                     MVADARPGVQNLTKSTLPEANRLVRDLRELTVSLRGFSERLEQGGIGGALGPEKLPDY
                     KPRKR"
BASE COUNT          528 a         1299 c         1164 g          520 t
ORIGIN      
        1 atggcacgat cccttcccca agccctgcta cctgttcgcg atggtcgccg gcgacctggt
       61 ctgcaaccgc gacagcttca ccacccggtc gggccgcgag gtctcgctcg gcatctgggt
      121 gcgcgaatcc gacctgccca agaccgctca cgcgatggac agtttgaagg cggcgatggc
      181 ctgggacgag cgggtctacg gccgcgaata cgacctcgac cagttcaaca tcgtcgcggt
      241 cagcgacttc aacatggggg cgatggagaa caagggcctc aacatcttca attcgcgcta
      301 tattctggcc gataaggaaa ccgctacgga ctccgatttc gacgccgtcg ccggggtcgt
      361 gggccatgaa tatttccaca attggtcggg caaccgggtc acctgccgcg actggttcca
      421 gctgtcgttg aaggaaggct tcaccgtctt ccgcgaccag agcttctcgg ccgacatcgg
      481 cagcgtcgcg gtcaagcgca tcgaggacgt gcggctgctg cgcgcggtcc agttccccga
      541 ggacgacggc ccgctcgccc atccggtgcg gcccgacagc tacctcgaaa tctccaattt
      601 ctacaccgcc accgtctaca acaagggcgc cgagctgatc cgcatgttcc acacattgct
      661 cgggcccgag atattccgcg ccggcaccga cctctatttc gatcgccacg acggccaggc
      721 ggcgacctgc gacgatttcg tccaagcgct cgaggacgcg agcggcgtcg acctcgagcc
      781 gttcaagatc tggtacgccc aggccggcac tcccaaggtc accgcgcggc tcgaccatga
      841 ctccgccgcc cggaccgcga ccctgcgcct cgcgcagcgc gtcgagccga ccccgggcca
      901 gccggtcaag tcgccgatgc cgatcccgct ccgcaccgcg ctgatcggtg cgaacagcgg
      961 cggcgaggtc gcgcccgagc ggctgatcct gctcgaccag gccgagcaga gcatccgctt
     1021 cgacgaggtc gccgagccgc ccctgctgtc gatcaaccgc ggcttctcgg ccccggtgat
     1081 catcgaggcg cagcgcggcc ccgacgagct cgagctgctc gcccggcacg accgtgaccc
     1141 gttcgcccgc ttcgaggcga tgcaggagct gatgcttcgc gcgctgatcg ccgggacccg
     1201 cggcgagccg gtcgacacgg cgccgctggt ccgggccgcc tcgaacacgc ttcggtcgga
     1261 ggcgctcgac gacgcgttca aggccgaggt cgtcctgccg ccgtcggaaa gccagatcgg
     1321 cgaccgaatg gaccgcgtcg accccgatgc gatccacgcc tcgcgcgagc ggctgcgcgc
     1381 gactctcggc aaggagcttc gggccgagct cgacgcggtc cgggcgatcg tccccgccgc
     1441 cgacctcagc ccggcggcca agggcgcccg ccgcctgagg agcgtcgcgc tcgggctggt
     1501 cgccgccgcc gacccggccg agggggcggc gctggccaag gcgcagttcg atgccgccgc
     1561 caacatgacc gaccgccagg gcgcgctcgc catcctggtc tcgctcgacc ggcccgagcg
     1621 gcaggaggcg ctcgattcct tctaccggcg gttcgaggac gaccctttgg tggtcgacaa
     1681 atggttcgcg cttcaggcga ccgcgcagcg ggccgacacc atcgacgagg tcgagcgcct
     1741 cgccgcgcac cccgccttca ccctcgccaa ccccaaccgt ctgcgcgcgc tcgccggaac
     1801 cttcgccgcc aaccagtggg tgttccacca tcgctcgggg cgcggctacc gcttcgtcgc
     1861 cgacatgatc ctcgccgccg accggttgaa cccgcaggtc gccgcccggc tggtgccgcc
     1921 gttcggccgc tggaagcgct tcgacgacga tcgcgcggcg cggatgcgct ccgagctcga
     1981 gcgcatcgtc gccgcgcccg gcctcagcaa ggacgtctac gagcaggcca gcaagagcct
     2041 cgcctagtcc gtgcaaacgg acgccgacga tagtattcct gttccgcgcg attcgaggag
     2101 gcggacatgc aagcgaccat cgtcaaggcc ggcgaaaggg cgccgctcaa tgtgctcggc
     2161 atgccgcttc gtttcctgtg cgatgcgcgg gagacgggcg gggcctggtc gctgttcgag
     2221 gaggaagtcc cgctggggat gggcccgccg ccccatcggc acgactggga cgaggcctat
     2281 tacgtgctcg agggcgaggt cgagttccag atcgacggcg agcgggtgca atcgaaagcc
     2341 ggggacttca attacctgcc gcgcaacacc gtccacgcgt tccggggggc ctcgagctcg
     2401 cccgcgcggg tgctgatctt cgcagccccc gcgcatgcgt ccgaattctt cgaggaagtg
     2461 aacagcgagg tccgcagcct ccccgacgac ctggcgaaaa ttccggagat cggccgccgg
     2521 cacggcatcc agttcctgcc gccggaagcg ccgagggccg gttagcggcc ggttagcggg
     2581 cggctagcgc ccgatccagc ccgccgcttc gagggcgacc tggttggccg cctggttgag
     2641 cgccgggccg accgaggccg catcgccgct cgccgggacc ttggcttcga accggcgggt
     2701 ctcgacgcgc gtcccgcccg ccgccgacaa ggccccgtcg aagcggacga tcacggcctg
     2761 ctcggcggcg tcgtagccca tcttgtgcag ctggccggac accaccacgc ccgggtcgag
     2821 cagcgcctgc ttggggtcga gcaccacccg gccggtagtg cgccggaccg tctccgagag
     2881 caggtcctgg aacagccggt cgggcgtgtc gacccattgc aaatccttga tgtaggcgac
     2941 ttccatgtgg ctgacctgcg ccggcacccg caccgtgcgc agctccttgg cgacgaccgg
     3001 cacggaaatc gtcaccgctt cgccggcgct cgccgagcgc gccatctcgc cctgctccgc
     3061 ggcctgcggg gtcagcttca gaagcgtcgg cggggtcttg ccgccgccga gcagcccgcc
     3121 cagcgagcag gcgcccagcg cagccgccag aacgaccggc agggatgtcc gcagcagcag
     3181 cttcatcagc gtttcctcgg cttgtagtcg ggcagtttct ccggccccag ggcgccgccg
     3241 atgccgccct gctcgagccg ctcggagaag ccgcgcagcg acaccgtcag ctcgcgcagg
     3301 tcgcggacca gccggttggc ctcgggcagg gtcgacttgg tcagattctg cacgcccggc
     3361 cgcgcgtcgg cgaccatcgc gtccagattg ctcgtcgccc gctcgacccc ggcgatcgcg
     3421 cgcctgaggt cctgcgccgc cggccggcct tcctcgacga ccagccggtt ggtgctgtcg
     3481 gcgagcaccc cgacgcgctg cgcggcgatg c
//
LOCUS       DASTZO010000009         8518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1149459,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000009 DASTZO010000000
VERSION     DASTZO010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 8518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8518
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1149459"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..790)
                     /locus_tag="VFU20_00175"
     CDS             complement(<1..790)
                     /locus_tag="VFU20_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5284907.1"
                     /translation="MPSTLKPAPDPEQVVKDAIAALDTPRAPELLPDIERALRQAPQD
                     ARLWHLKGLIHRSQDRRELAIPALRRAAELAPNVPLIAHGLARTLNEAGLPSVDAYAR
                     ALRLSPGDTTLLTGLVAALLAEHRLADAMAGLERALERAPRWVEGHGMLSDLRWLEGE
                     REGFARSFDEALALHPSDMALRRGQIIALLHAERYDDVLARIADGRRAIGDDALFDVN
                     EAIVWSETGEAEKADRRFEQLKDLADSSTQVRRVRHLLRYGRPAD"
     gene            complement(793..1947)
                     /locus_tag="VFU20_00180"
     CDS             complement(793..1947)
                     /locus_tag="VFU20_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEU5284908.1"
                     /translation="MADDAITLEQAADRAATSGDPAAASRLLEQAVAQAGDKPGLWVK
                     LSAMRRAGGDLGGALEAVERALAIEPLNFSALLSRAFLLEGSGDPRAGEAFAVAVAQA
                     PPDESLPKPVASALEHARQRSAEHGAAVERRLRDAVGMDLPAAERARVERFVANTARR
                     TRHFHQEPSHFHFPGLPEVEFHDREEFPGLEALEARTGEIRAEFQALVASEAAQLVPY
                     IQYPDNVPLRQWKELNRNRQWTAIHLVQNGQRVEANARHCPRTLEAVSAFSQPQVPGA
                     SPNAMFSLLAPRTRIPPHTGVANTRLVCHLALIVPPECGFRVGATTVEWKEGTAFVFD
                     DTIEHEAWNDSDQLRVVLIFDLWAPALSDAERRAVAAIIPAAGVGFGGGI"
     gene            complement(2033..5110)
                     /locus_tag="VFU20_00185"
     CDS             complement(2033..5110)
                     /locus_tag="VFU20_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5284909.1"
                     /translation="MKLDFRQRLLATTLLVGASMVASPALAQDVAPAAPCPPDSPNCV
                     PETTAPVEGTTLTTATPSAEGEAVESTEDIIVTGTRIPQPNLTSASPVTVVTSQEVKL
                     SGTTRTEDLINSLPQSFAAQGSNISNGATGTATVNLRGIGTSRTLVLVNGRRLHPGDP
                     RAPAPDLNFIPAALVKRVDVLTGGASSVYGADAVAGVVNFIIDTEFRGLRIDGQASAF
                     MHSNDGDPDALAANDARGFRYPTGQSVNGGAQDISVSLGAGFDDNRGSVMAYATYRNQ
                     SSVLQSTRDYSFCALTANSQATITSTGRRFNCGGSATSANGTFFTNVGTFQVGPGQTF
                     IPGSTPFNFAPYNYYQRPNERYTLGAFANYEIDEMFNPYLEAMFMDDHTDAVIAPSGN
                     FFSTNTINCDNPLLSTQQFNTICVPANTFVDADGVTQAVAYTGRRNVEGGGRQDDVEH
                     ISFRVVAGMRGDLDRGLSYNASGVFGKTILSQIYRNDFSVTRLNRAMDVVTDPATGLP
                     VCRSVLTGEDPACVPWNIFIEGGVTQEALDYLQTPGLSRGNVQQKSAGIDFTFLGGEY
                     GLQLPWSDRGVSANVGGQYIKHSLLFETDTAFSTGDLAGQGGPTIGVSGDYDVRELFG
                     EVELPIVSNSFIEEFTVRAGYRKSWYHVADNKFSTDTYKIEAELAPVRDIRLRGSYNR
                     AVRAPNVIELFSAQSIGLGGSDDPCAGAVPTATLAQCQLTGVTAAQYGSIIPNPAEQY
                     QSLFGGNPDLSPEKADSYTFGLVLQPRFIPGLAVTVDYFDIRLRNAIGVIGFDTIMAQ
                     CLSTGDPFFCSKINRAPGTGSLWLPVVDPSQGGFISDVNTNVGGLKTRGVDVQASYAR
                     QIGRLGNLNLSMVGTWLDKLEVNPVADTKYDCKGFFGNQCGTPNPKWRHKARAGITLP
                     NGIGISGQWRYFSSVKVDGLSSDPDLAVPPTSPLVRPANRKLNAISFFDLALQARITD
                     RYNFRMGANNILDTDPPIAGSQVIFAGSGNGNTFPQVYDSLGRFLFAGFTVDF"
     gene            5336..5866
                     /locus_tag="VFU20_00190"
     CDS             5336..5866
                     /locus_tag="VFU20_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284910.1"
                     /translation="MKRKIIFAAAACALLSTAAAAAPRKKAEGGAPAQIQRLLGCRAL
                     ADAAQRLACFDRETSALDQAMAKRDVVVIDRQRVTAARRSLFGFSVPSFGGLFGGDEN
                     EVKEIQSTVAAVARNPEGGWTIKLADGSTWTQTDDTPIGLRPERGQKIVVRRGSLGSF
                     RLSVNGQPGFKVKRIG"
     gene            complement(5879..6094)
                     /locus_tag="VFU20_00195"
     CDS             complement(5879..6094)
                     /locus_tag="VFU20_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2093 domain-containing protein"
                     /protein_id="HEU5284911.1"
                     /translation="MLMRGAGKTARLQFLPSTFRVLSDGDHVVCAVTGAVIPLHELRY
                     WSVERQEAYRDAAASLEAEQRAAKAPG"
     gene            complement(6100..7509)
                     /gene="xseA"
                     /locus_tag="VFU20_00200"
     CDS             complement(6100..7509)
                     /gene="xseA"
                     /locus_tag="VFU20_00200"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU5284912.1"
                     /translation="MDLPDDTADDKSAGLLAEVAAGDNSPPLTVSELSGALKRTIETA
                     FGQVRVRGEISGFKRHASGHCYFTLKDDAACIDAVIWRGQAASLAFRPEDGAEVIATG
                     RLTTYAGRSKYQIVVDRLELAGEGALMALLERRRKALAAEGLFDAARKRKLPFLPQLI
                     GVVTSPTGAVIRDILHRLEDRCPTHVIVWPVPVQGEGAAGRIAAAIRGFADLTPRPDL
                     LIVARGGGSIEDLWAFNEEEVVRAAAQSPIPLISAVGHETDTTLIDHAADLRAPTPTA
                     AAELAVPVRAELFALLADLGRRAAHCLAARAERARERLDLTACRWPRPEALFAPAVQR
                     ADELGERLPRALAARAGHARADLNAVAPRLRPQLLQDRIERAAEKLAALWKMAGLVHP
                     DRPLSKGFARVTSRAGQTLTRAADALAERLLTLHFGDGRVDAVAGDQAPPGAPARVER
                     SRRRAYLPPQPGLFDEPGE"
     gene            7508..>8518
                     /gene="purD"
                     /locus_tag="VFU20_00205"
     CDS             7508..>8518
                     /gene="purD"
                     /locus_tag="VFU20_00205"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU5284913.1"
                     /translation="MNILLLGSGGREDALAWRLRQSPSCEELIAAPGNPGIARWAQCV
                     AADPMDPDAMIELARRRSIDLVVIGPEAPLVAGVGDSLRAAGFAVFGPNADAARLEGS
                     KGFTKDLCRANGIPTADYVRSGTMAEARAALERFGAPVVVKADGLAAGKGVTVAMTLA
                     EAEGAIAAAGDGPLVIEEFLEGEEASLFALVDGATAVPLASAQDHKRVGEGDTGPNTG
                     GMGAYCPAPVLTPELEARAMEEIVRPTARALAAAGTPFSGVLYAGLMLTRDGPRLIEY
                     NVRFGDPECEAILPLVEGDFAELLNAVATGRLADIEPPRLSGRHAVTVIVAARGYPGT
                     PAS"
BASE COUNT         1280 a         2815 c         3136 g         1287 t
ORIGIN      
        1 catcggcggg ccgcccgtag cgaagcagat ggcggacccg gcggacctgc gtcgagctgt
       61 cggcaaggtc cttcaattgc tcgaaccggc ggtcggcctt ctcggcctcg ccggtctccg
      121 accagacaat ggcttcgttg acgtcgaaca gggcgtcgtc gccaatcgcg cgccggccgt
      181 cggcgatccg ggccaggacg tcgtcatagc gctctgcgtg gagcagggcg atgatctgcc
      241 cgcggcgaag cgccatgtcg ctcgggtgca gcgcgagcgc ctcgtcgaag ctgcgcgcaa
      301 agccctcccg ctcgccctcc agccagcgca ggtcgctgag catgccatgg ccttccaccc
      361 accgcggcgc ccgctccaac gcgcgctcca ggccggccat ggcgtccgcc aggcggtgct
      421 ccgcgagcaa ggccgccacc aggccagtga gcagcgtcgt gtcgcccgga gacagccgca
      481 gcgcccgggc ataggcgtcg acgctgggca ggccggcctc gttgagggtc cgcgccaggc
      541 cgtgggcgat caacgggacg ttgggcgcca gctcggccgc ccgccgaagc gccgggatcg
      601 cgagctcgcg ccggtcctgc gaacggtgga tgaggccctt caaatgccac agcctggcgt
      661 cctgcggggc ctggcgaagc gcgcgctcga tgtccgggag cagctcgggc gcccgtggcg
      721 tgtcgagcgc cgcgatcgcg tccttgacca cctgctccgg gtcgggcgcg ggcttgagcg
      781 tgctcggcat gcctagatcc cgccgccgaa gccgacgccg gccgccggga tgatcgccgc
      841 gaccgctcgg cgctcggcgt cgctgagggc cggcgcccac aggtcgaaga tcaggaccac
      901 gcgcagctgg tcgctgtcgt tccacgcctc atgctcgatg gtgtcgtcga acacgaacgc
      961 cgtgccctcc ttccactcga cggtggtcgc gccgacccgg aagccgcact cgggcggcac
     1021 gatcaaggcg aggtggcaga ccaggcgggt gttggcgacg ccggtatgcg gcgggatccg
     1081 ggtgcgcggc gcgagcagcg agaacatcgc attgggcgat gcgccgggca cctgcggctg
     1141 ggagaatgcg gagaccgcct cgagcgtccg cgggcagtgg cgcgcgttgg cctcgacccg
     1201 ctggccgttc tggaccaggt ggatggccgt ccattgccgg ttgcggttga gctccttcca
     1261 ctgccgcagc gggacattgt ccggatactg gatgtacggc accagctgcg cggcttcgct
     1321 ggcgaccagg gcctggaatt cggcgcggat ctcgccggtg cgcgcttcca gcgcctcgag
     1381 gccggggaat tcctcgcggt cgtggaactc gacctccgga aggcccggga agtggaaatg
     1441 gctcggctcc tggtgaaagt gcctggtgcg gcgggcagtg ttggcgacga accgctcgac
     1501 ccgggcgcgc tcggccgccg gcagatccat gccgacggcg tcgcggagcc gccgctcgac
     1561 ggccgcgcca tgttcggcgc tccgctgccg cgcatgctcc agcgcggatg cgacgggctt
     1621 gggcagggac tcgtccggcg gcgcctgggc gacggcgacg gcgaaggcct cgccggcccg
     1681 gggatcgccc gatccctcga gcaggaaggc gcgggagagg agggccgaga aattgagcgg
     1741 ctcgatggcc agggcccgct cgaccgcctc cagcgcgccg ccgaggtcgc cgccggcccg
     1801 gcgcatcgcc gacagcttga cccacaggcc gggcttgtcg ccggcctggg ccaccgcttg
     1861 ctcgagcagg cgcgaggcgg ccgcgggatc gcccgacgtc gccgcccggt ccgccgcctg
     1921 ttccagggtg atcgcatcgt cggccatcgg cgcattcatg gcccgtcgcg ccgcaaaaga
     1981 aaacggcgga cctctttcga ggcccgccgc aatccttgtc ttgccgcaga ctttagaagt
     2041 cgacggtgaa gcccgcgaac aggaaccgtc ccagcgagtc atagacctgc gggaaggtgt
     2101 tgccgttacc ggagccggcg aagatgacct ggctgcccgc gatcggcgga tccgtatcga
     2161 ggatattgtt ggcgcccatc cggaaattgt accggtcggt gatccgggcc tgaagcgcga
     2221 ggtcgaaaaa gctgatcgcg ttcagcttgc ggttcgccgg gcggaccagc ggcgacgtcg
     2281 gcggcactgc cagatccggg tcgctgctca acccgtcgac cttgacgctc gagaagtatc
     2341 tccactggcc cgagatgccg atgccgttgg gcaaggtgat gcccgcgcgg gccttgtggc
     2401 gccacttggg attgggcgtg ccgcactggt tcccgaagaa gcccttgcag tcgtacttgg
     2461 tgtcggcaac cgggttgacc tcgagcttgt cgagccatgt gccgaccatg ctcaggttca
     2521 gattgcccag acgtccgatc tggcgggcgt agctggcctg gacgtcgacg ccgcgggtct
     2581 tcaagccgcc gacgttggtg ttgacgtcgc tgatgaaccc accctgcgac gggtcgacga
     2641 ccggcaacca cagcgagccc gtgcccggcg cgcggttaat cttcgagcag aagaagggat
     2701 cgccggtgga aaggcactgc gccatgatcg tgtcgaagcc aatcacgccg atcgcattcc
     2761 gcagccggat gtcgaaataa tcgacggtga ccgccaggcc ggggatgaag cgcggctgaa
     2821 ggacgagtcc gaacgtgtag gagtcggctt tctcgggcga cagatccggg ttgccgccga
     2881 acagggactg gtattgctcg gccgggttgg ggatgatgct cccgtattgc gccgcggtca
     2941 cgccggtcag ctggcactgg gcaagcgtcg cggtaggcac ggcacccgcg cacgggtcgt
     3001 ccgagccgcc gagaccgatc gactgggcgg agaacaactc gatcacgttg ggcgcccgga
     3061 ccgcgcggtt gtagctgccg cgaagccgga tgtcgcgcac cggagcaagc tcggcctcga
     3121 tcttgtaggt gtcggtcgag aacttgttgt cggcgacatg gtaccaggat ttgcggtagc
     3181 cggcgcggac cgtgaattcc tcgatgaagc tgttgctgac gatcggcaac tcgacttcgc
     3241 cgaacagctc gcgcacgtca tagtcgcccg agacgccgat ggtcggcccg ccctggccgg
     3301 cgagatcgcc ggtcgagaaa gcggtgtccg tctcgaagag cagcgagtgc ttgatgtact
     3361 ggccgccgac gttggcgctg acgccgcggt cggaccatgg cagctgcagg ccatattcgc
     3421 cgccgaggaa ggtgaagtcg atcccggcgc tcttctgctg gacgttgccg cgcgagaggc
     3481 ccggagtctg aaggtagtcg agcgcttcct gggtcactcc gccttcgatg aagatgttcc
     3541 agggcacgca ggcgggatcc tcgccggtca gaaccgaacg gcagaccggc aggccggtcg
     3601 ccggatcggt gacgacgtcc atcgcccggt tgagccgggt gacggagaag tcgttccgat
     3661 aaatctggga aaggatggtt ttgccgaaca cgccggaggc gttgtacgac aggccccggt
     3721 ccaggtcgcc gcgcatgccg gcgacgacgc ggaagctgat gtgctcgacg tcgtcctggc
     3781 gcccgccgcc ctcgacgttg cggcggccgg tgtaggcgac cgcctgggta acgccgtcgg
     3841 cgtcgacgaa ggtgttcgcc gggacgcaga tcgtgttgaa ctgctgcgtc gacagaagcg
     3901 gattgtcgca gttgatcgtg ttggtgctga agaagttgcc cgacggggcg atcaccgcgt
     3961 cggtgtggtc gtccatgaac atcgcttcga gatacgggtt gaacatctcg tcgatttcat
     4021 aattggcgaa cgcgcccagc gtgtagcgct cattcggacg ctggtaataa ttgtacggcg
     4081 cgaagttgaa cggcgtggag ccgggaataa aggtctgacc cggaccaacc tggaaagtgc
     4141 cgacgttggt gaagaaggtg ccgttcgccg atgtcgccga gccgccgcag ttgaaacggc
     4201 gaccggtcga agtgatcgtg gcttggctgt tggcggtcag cgcgcagaag gagtaatcgc
     4261 gggtcgactg cagcaccgag ctctggttgc ggtaggtcgc ataagccatg acgctgccgc
     4321 ggttgtcgtc gaagccggcg ccgagcgaga cggagatgtc ctgggcgccg ccgttgacgc
     4381 tttgcccagt cgggtagcgg aagccgcggg cgtcgttggc ggccagggca tccggatcac
     4441 cgtcgttgct gtgcatgaac gcgctggcct ggccgtcgat tcgaaggccg cggaactcgg
     4501 tgtcgatgat gaagttgacg acgccggcaa ccgcgtcggc gccgtacacc gacgaagcgc
     4561 cgccggtcag gacgtcgacc cgcttgacca gggccgccgg aatgaagttg aggtcaggag
     4621 ctggcgcgcg cgggtcgccc gggtgcaggc gacggccgtt gaccagcacc aacgtgcgcg
     4681 acgtgccgat gccgcgcagg ttgacggtcg cggtgccggt ggcgccgttg gaaatgttcg
     4741 agccctgggc ggcgaacgat tgcggcagcg agttgatcag atcctcggtg cgggtcgtgc
     4801 cggaaagctt gacttcctgc gaggtgacga cggtcaccgg gctcgccgac gtcaggttcg
     4861 gctgcggaat gcgcgtgccg gtgacgatga tgtcctcggt ggactcgacg gcctctccct
     4921 cggccgacgg agtcgcggtc gtgagggtcg tgccttcgac cggagcggtc gtctcgggca
     4981 cgcaattggg cgaatccggc gggcagggag ccgcaggagc gacgtcctgg gcgagggcgg
     5041 gcgaggcaac catgctggcg ccaacgagga gggtcgttgc gagcaggcgc tggcggaaat
     5101 cgagcttcat agaatgtctt ccccttgatg ggccgtgcct tggacacggc cgcagtgccg
     5161 cgacaatgcg agttgccgta actgccctac ctgataggtg aagcggcaag acgctggcaa
     5221 tgttcgcggc ccgccgcccg tgaagttgtc gggcggctgt ctcaattttg caacaggcgc
     5281 ccttgccctt ggaggggctc cgacgatagt gatcgtggcc agggagactc ttcaaatgaa
     5341 acggaaaatc attttcgccg cggcggcctg cgcattgctg tcgacggccg ccgcggcggc
     5401 cccgcgcaag aaggccgagg gcggcgctcc ggcgcagatc cagcggctgc tcggctgccg
     5461 ggcgctggcc gacgcggcgc agcggctcgc ctgcttcgac cgcgagacct cggcgctcga
     5521 ccaggcgatg gcgaagaggg acgtggtggt gatcgaccgc cagcgggtga ccgcggcgcg
     5581 gcgttcgctg ttcggcttct ccgtgcccag cttcggcggg ctgttcggcg gcgacgagaa
     5641 cgaggtcaag gaaatccaga gcaccgtcgc ggccgtcgcc cgcaacccgg aaggcggctg
     5701 gaccatcaag ctcgccgacg gctcgacctg gacccagacc gacgacacgc cgatcggact
     5761 gcggcccgag cgcggccaga agatcgtcgt tcgccgcggt tcgctcggca gcttccggct
     5821 gagcgtcaac ggccagccgg ggttcaaggt caagcggatc ggctaggcgc ggaacccgct
     5881 agcccggggc cttggcggcg cgctgctcgg cctcgaggct ggcggcggcg tcgcgatagg
     5941 cctcctgccg ctcgacgctc cagtagcgca gctcgtgcag cgggatgacg gcgccggtca
     6001 ctgcgcagac cacatggtcg ccgtcggaca gcacccggaa ggtgcttggc agaaattgca
     6061 gccgggcggt cttcccggcg cccctcatca gcatatggct cactccccgg gctcatcgaa
     6121 caggcccggc tggggcggca gataggcgcg ccgccggctc cgctcaaccc gggccggggc
     6181 gcccggcggc gcctggtcgc cggcgaccgc gtcgaccctg ccgtcgccga aatgcagggt
     6241 cagaagccgc tcggcgagcg cgtcggcggc gcgcgtcagg gtctggccgg cgcggctggt
     6301 gacccgggcg aagcctttcg acaagggccg gtcgggatgg accagcccgg ccatcttcca
     6361 cagcgcggcg agcttttccg cggcccgttc gatccggtcc tgaagcagct gcggccggag
     6421 ccgcggggcg accgcgttca aatcggcgcg cgcgtggccc gcacgcgcgg cgagcgcgcg
     6481 aggcagccgt tcgccgagct cgtcggcgcg ttgcaccgcc ggcgcgaaca gggcctcggg
     6541 ccgcggccag cggcaggcgg tgaggtcgag ccgctcccgg gcccgctcgg cgcgggcggc
     6601 caggcaatgc gccgcccttc ggccgaggtc ggcgagcagg gcgaacagct cggcccggac
     6661 cggcaccgcc agctcggcgg cggcggtcgg ggtcggggcg cgcaaatcgg cggcgtggtc
     6721 gatcagcgtg gtgtcggtct cgtgcccgac cgccgagatc agagggatcg gcgattgcgc
     6781 cgcggcgcgc accacctcct cctcgttgaa cgcccacaga tcctcgatcg agccgccgcc
     6841 gcgggcgacg atcagaaggt ccgggcgcgg agtgaggtcc gcaaagccgc ggatggccgc
     6901 ggcgatccgg cccgccgcgc cctcgccctg gaccggcacc ggccagacga tcacatgggt
     6961 cgggcagcgg tcctcgagcc ggtggaggat atcgcggatc accgccccgg tcggcgacgt
     7021 caccaccccg atcagctgcg gcaggaacgg cagcttgcgc ttgcgcgcgg cgtcgaacag
     7081 cccctcggcg gccagcgcct ttcggcggcg ctcgagcagc gccatcaggg cgccctcgcc
     7141 ggccagctcc agccggtcga cgacgatctg atatttggac cggccggcgt aggtggtcag
     7201 ccggccggtg gcgatgacct cggcgccgtc ctccggtcgg aaggcgagcg acgccgcctg
     7261 gccgcgccag atcaccgcgt cgatgcacgc cgcgtcgtcc ttcagggtga aatagcaatg
     7321 gcccgacgcg tggcgcttga agcccgagat ctcgccgcgc acccggacct ggccgaacgc
     7381 cgtctcgatg gtccgcttca aggcccccga cagctcgctg acggtcagcg gcggcgaatt
     7441 gtcgccggcc gccacctcgg ctaacaggcc cgcactcttg tcgtcagccg tgtcgtcggg
     7501 gaggtccatg aacatcctgc tgctgggttc gggcggccgc gaagatgcgc tcgcctggcg
     7561 cctgaggcaa tcgccaagct gcgaggagct gatcgccgcg cccggcaacc cggggatcgc
     7621 gcgctgggcg caatgcgtgg cggcggaccc gatggatccg gatgcaatga tcgagctggc
     7681 gcgccgccgc tcgatcgacc tggtggtgat tggccccgag gcgccgctgg tcgccggcgt
     7741 cggcgactcg ctcagggcgg cgggattcgc ggtgttcggg cccaacgccg atgccgcccg
     7801 gctcgaaggc tcgaagggct tcaccaagga tttgtgccgc gcgaacggca tcccgaccgc
     7861 cgattatgtg cgctccggga cgatggccga ggcgcgcgcc gcgctcgagc ggttcggggc
     7921 gccggtggtg gtcaaagccg acgggctcgc cgcgggcaag ggagtcaccg tcgcgatgac
     7981 cctggctgaa gccgaaggcg cgatcgccgc ggccggcgac gggccgctgg tgatcgagga
     8041 gttcctcgag ggcgaggaag ccagcctgtt cgctttggtc gacggcgcga ccgcggtgcc
     8101 gctcgcctcg gcccaggacc acaagcgggt cggcgagggc gacaccgggc ccaacaccgg
     8161 cggcatgggc gcctattgcc cggcgccggt cctgacgccc gagctcgagg cccgcgcgat
     8221 ggaggagatc gtccgaccga cggcgcgggc gctggcggcg gccgggactc cgttttccgg
     8281 agtgctctat gcggggttga tgctgacccg cgatggcccc aggctgatcg agtataacgt
     8341 ccgcttcggc gaccccgaat gcgaggcgat cctgccgctg gtcgagggcg atttcgccga
     8401 gctgctgaac gcggtggcga ccgggcggct ggcggacatc gagccgccgc ggctgtcggg
     8461 gcgccatgcg gtgaccgtga tcgtcgccgc gcgcggctac cccggcaccc cggcgagc
//
LOCUS       DASTZO010000010         8355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_601197,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000010 DASTZO010000000
VERSION     DASTZO010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_601197"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="VFU20_00210"
     CDS             complement(<1..129)
                     /locus_tag="VFU20_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA methyltransferase"
                     /protein_id="HEU5284914.1"
                     /translation="MSRGGGGGHQRVRTARGRTASSTRWLERQLNDPYVRRAKADNY"
     gene            complement(146..1198)
                     /locus_tag="VFU20_00215"
     CDS             complement(146..1198)
                     /locus_tag="VFU20_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HEU5284915.1"
                     /translation="MAQTVAGTPALLHGESGQSGPAYKPRERRQQRTSYGAVDLGTNN
                     CRLLIARPVDGGFTVVDAFSRVVRLGEGLSQSGRLTDDAMDRAVAALAICGEKLRRRN
                     VSLSRAVATEACRRAVNGREFVSRVRRETGIALDIISPQEEARLAVLGCHKVLAPGDG
                     PALIFDIGGGSTELVLIDHIDGMPKIRSWWSAPWGVVSLTESEGRTAIDGKDRLAAYK
                     RMRERARHSFSRFAETLPKTSADIRLLGTSGTVTTLASVHLALPSYDRRAVDGLHVPT
                     EAMQKISTMIAGMDYEERSRLPCIGRDRADLVVAGCAILEAILEIWPAPSLGVADRGI
                     REGILRSLMARDGHLL"
     gene            1379..1449
                     /locus_tag="VFU20_00220"
     tRNA            1379..1449
                     /locus_tag="VFU20_00220"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1411..1413,aa:Gln,seq:ctg)
     gene            1692..3407
                     /locus_tag="VFU20_00225"
     CDS             1692..3407
                     /locus_tag="VFU20_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284916.1"
                     /translation="MGAPALKSRFVLVATVVTGCTCCVGTRAAEDAAASRSAPLGGEI
                     IVAGQFAATGPRYSPDGTRIAFTARRGSKNDIAIVPSAGGPPTFVTKGGSNIHPAWSP
                     DATRLVYVSHSGGEADLYVIPAAGGAAVRITHSGAAKAAPDWSPDGRTIAYSSSEGGD
                     WNIWSVPASGGPARKLTRHRGDEWNPRYSPDGRSILFSTNWGENANIDTWAIPGGGGE
                     PVRLTFGPEDEVTAAWSPDSRRIAFLTDLGGLFVMDLASGSRTNVAPGEGFQDILSWS
                     LDGRWIAVGRNPKPYRLFQVSLDGGEPRPLGLAEWNVWTPDVSRISGAIAFAAMDRSG
                     NGDVQLLRGPGSRLVRFTANPAPDLHPAWSPSGKQIAFTSRRDGAHNGDIWVAPTTKG
                     GPAVRLTWLRSAHRPRWCSDNVIVFHSDRGADGQPHVWAVERGKAPRQLTYGRREVEP
                     DCVPATGDVLYVGSSSRGSELFRTTLVGGQATQLTHDGASARFPRVSRNGAWIAFVST
                     REGPSEIFIMPSGGGPARRLTSDGGEKSPPAWSSDGKSLVYSVKRGEPQIRRYAAPAC
                     SLHSC"
     gene            3647..3844
                     /locus_tag="VFU20_00230"
     CDS             3647..3844
                     /locus_tag="VFU20_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284917.1"
                     /translation="MDFDTLDYARMSEEAMAAADGSDTLEECQAHLDRAVRYAQLACL
                     ERHRPGRSNVVDIASARRAAR"
     gene            complement(3846..6539)
                     /locus_tag="VFU20_00235"
     CDS             complement(3846..6539)
                     /locus_tag="VFU20_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5284918.1"
                     /translation="MRRTRFAGAVEIPGEAKSRASIREALWFDILAPDTPDEHSLSAI
                     RVASLDYCALMLGITHLIAAIALVALHPERALSTSLANPVIPMLMLLALDFVAIVGLQ
                     WRDRLNLAPHTVIRALAVYLAAAGCLWILVGLAMSAQAHVGHASFPILLIGGGITMAA
                     MVAVNSPALALANSLVTTAGAFVLTGSVLVGGGAALLCLIIVAYSVAGAQTIIAAGRK
                     RLNLDAEARKALTFVAEFENSGRGWFWETNSEGTLSYVSQQLADDFQCEPQALLGRPF
                     ADLLSVDTSPVGSIHEERKTLGFHLSARFPFADVVVRPASDQDVHWSLSGNPIFDERG
                     RFLGFRGIGTDLTEQRRSEQEISRLARFDSLTGLPNRALMRQTLDEALRNAASRQKGC
                     ALFLIDLDRFKNVNDTLGHPIGDALLRQVAERLKTVMGDHGQVGRLGGDEFKAVLPGT
                     VDIGFLEPLARTLIEQVSRPYTIEGHRVMIGASVGIAIGDPGRACADSLIRNADLALY
                     AAKAAGRGKHCFYEPAMHSDLSDRQVLENDLRQAIDRGELWVAYQPIVRVAGEEICGF
                     EALVRWNHPVRGPISPDKFIPLAEECGLIPGIGKWVLRTALLEAADWPDQVRVAVNLS
                     PIQFNDPGIVGIVAELLKETGVRSERLELEITEGVFLAEGEGTDETFAGLKKLGVRLA
                     LDDFGTGYSSLGYLKKAPFDKIKIDQSFVRGAASTTNRNSAIIRAIVTLADTLGMDTT
                     AEGVETHDDLHLIRELGCSQVQGYIFGRPAAAEEARQLANLVTVEADGYQCIREPRQR
                     LMRRAVAEIDGQVTEIRLRNISAMGALVECAQPISPGTVITIDIVGVGPVVGSVRWSQ
                     SGKFGVQFTDTFDLARLAPKKERLNEVTMLRPWYIDQREAS"
     gene            complement(6641..7567)
                     /gene="cysK"
                     /locus_tag="VFU20_00240"
     CDS             complement(6641..7567)
                     /gene="cysK"
                     /locus_tag="VFU20_00240"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEU5284919.1"
                     /translation="MKAASILETIGNTPHIRVNRLFGDNAEVWIKSERSNPGASIKDR
                     IALSMVEAAEASGALKPGGTIVEPTSGNTGIGLAMVAAVKGYRLVLVMPDSMSIERRR
                     LMLAYGATFDLTPREKGMKGAIARAEEIVAETDGAWMPQQFENPANLEVHRRTTAREI
                     LSDFRDSPIDVLITGVGTGGHITACAEVLKREWPALRVFAVEPTLSPVLSGGEPGPHP
                     IQGIGAGFVPAIMDTGLLDGVIQVDPADAKEMARRAAREEGVLVGISSGATLAAIARK
                     LPELGEAPRVLGFNYDTGERYLSVPDFLPEEA"
     gene            complement(7564..>8355)
                     /locus_tag="VFU20_00245"
     CDS             complement(7564..>8355)
                     /locus_tag="VFU20_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5284920.1"
                     /translation="GPALGGFLYAASPSAPYWAAALLMAVSILALTPIRRIAPPPMVE
                     AHPLRQMADGLSYVRHHRFLFGAITLDLFAVLLGGATAMLPVFARDILHVGTEGLGLL
                     RAAPAVGAAIVALWFAWRPLRHNVGVKMILAVAAFGVMTILFGLSREMWLSLAALALL
                     GGFDMLSVYVRSSLVQLHTPDTMRGRVSAVSGLAISASNELGELQSGIAAAILGPVGA
                     VLFGGAAAIGIAGLWSYLFPELARARTFEPPATALPFAVMEEKPA"
BASE COUNT         1352 a         2740 c         2918 g         1345 t
ORIGIN      
        1 gtaattgtcg gccttggcgc ggcgcacata agggtcgttg agctgccgct cgagccaccg
       61 ggtcgacgag gcggtgcggc cccgcgcggt gcgcacgcgc tggtggccgc cgcctccccc
      121 ccggctcacg aaaaggcgcc ccggctcaca agaggtggcc gtcgcgcgcc atcagcgatc
      181 gcaatatgcc ctcgcggatg ccgcggtcgg cgaccccgag gctcggcgcc ggccagatct
      241 cgaggatcgc ctcgaggatc gcgcagccgg cgaccaccag gtcggcgcgg tcgcggccga
      301 tgcacggcag gcgcgagcgc tcctcgtagt ccatcccggc gatcatggtg ctgatcttct
      361 gcatcgcctc ggtcgggacg tgcagcccgt cgaccgcccg gcggtcgtag gacggcagcg
      421 ccaggtgcac gctggcaagc gtggtgacgg tgccgctggt gccgagcagg cggatgtccg
      481 ccgaggtctt ggggagcgtc tcggcgaagc ggctgaacga gtggcgggcg cgctcgcgca
      541 tgcgcttgta ggcggccagc cggtccttgc cgtcgatcgc cgtccggcct tcgctttcgg
      601 tcagcgagac gacgccccac ggagcgctcc accagctgcg gatcttgggc attccgtcga
      661 tatggtcgat cagcaccagc tcggtcgagc cgccgccgat gtcgaagatc aaagccgggc
      721 cgtcgccggg agcgagcacc ttgtggcagc cgagcaccgc cagccgggcc tcctcctgcg
      781 gcgagatgat gtcgagcgcg atcccggtct cgcgccgcac gcgcgatacg aactcgcggc
      841 cgttgaccgc ccgccggcat gcctcggtgg ccaccgcgcg cgacagcgaa acattgcggc
      901 ggcgcaattt ttcaccgcag atggcgagcg ccgcgaccgc ccggtccatc gcgtcgtcgg
      961 tcagccgacc gctctgcgac aggccttcac caagcctcac cacgcgcgaa aaggcgtcga
     1021 caacggtgaa gccgccatcg accgggcggg cgatcaggag gcggcaattg ttggttccga
     1081 ggtcgaccgc gccatagctg gtgcgctgct gccgccgctc tcgcggcttg tatgctggcc
     1141 cgctctggcc gctttccccg tgcagcagag ccggcgtgcc ggcgactgtc tgcgccatgt
     1201 ccaacgcccg ttctatcctt cgccgcccgg taaaaagacg gctgacttaa acccgcttaa
     1261 tcacattaag caggccgaac gcaagttttg ggccgttgga aggtgtcggc gcgggcgccc
     1321 gggccgagcc cccgcgttga cagcgtgtgg gggcctttct atctcgccca ctcgccgctg
     1381 ccccgtcgtc taatggtaag actacggact ctgaatccgt caatcgaggt tcgaatcctc
     1441 gcggggcatc cggcctatcc cgacaatgcg ttgatattgc cagctttatt ggaaatagca
     1501 gtcgggcgct ccctcgcttt ctcccacaaa cagccgagga ttacgcagtg tccgctttcg
     1561 acccaaagcg gacgtcccga agtggatcgg ctagtggact aagttgagct gggcgcttgg
     1621 agtcctgcgg gcgttccgga tattatgggg cggcatcgtc tggacccgag cttcgctaag
     1681 aggagacggc aatgggtgct ccggcgctga agagccgttt tgttttggtg gccaccgtgg
     1741 ttaccggttg cacctgttgc gtcgggacgc gcgcggcaga ggatgcggca gccagcaggt
     1801 ccgcgccgct gggcggcgaa atcatcgttg ccgggcaatt cgcggctaca ggtccgcgct
     1861 actctccaga cgggactcga atcgccttta ccgcgaggcg aggcagcaaa aatgacatcg
     1921 ctattgtgcc aagcgccggt gggccgccaa cgtttgtcac caagggcggc agcaatattc
     1981 atcctgcctg gtcgcccgac gcaactaggc tcgtttatgt ttctcatagc ggtggggagg
     2041 ccgaccttta cgttatcccc gcggcgggag gggcggcggt ccgcatcaca cattcgggcg
     2101 cggccaaggc cgcgcccgac tggtcaccgg acgggcggac cattgcatat tcgtctagcg
     2161 agggcggcga ctggaacatc tggtcggtgc cggcctcggg cggccctgct cgaaagctca
     2221 cgaggcaccg tggagacgag tggaacccgc gctactcgcc agacggacgg agcattctct
     2281 tttccaccaa ctggggagag aacgcgaaca tcgacacctg ggcgattccg ggtggcggcg
     2341 gcgagccggt ccgcctcaca ttcggccccg aagacgaagt cactgccgca tggtcgccag
     2401 atagtcggcg catcgcgttt ctcaccgacc ttggtgggct gttcgtcatg gaccttgcgt
     2461 ccggaagtcg gacaaatgtc gctcccggcg agggtttcca ggatatactc tcctggtcgc
     2521 tcgacgggcg gtggattgcc gtcggtcgga accccaagcc ttaccggctg ttccaggtgt
     2581 ccttggatgg aggcgagccg cgcccgctcg gtctggccga atggaacgtc tggacacccg
     2641 acgtttcgag aatcagcggc gcgatcgcct ttgcagccat ggatcgctcg gggaacggcg
     2701 atgtgcaact gctgcgcggg cccggctcaa ggctcgtgcg gttcaccgcc aatcccgcac
     2761 cggacttgca tcctgcctgg tcgccgtcgg ggaagcagat tgcttttaca tcacggcgcg
     2821 atggggctca caatggagac atttgggtcg cgccgacaac aaaaggcgga cccgccgttc
     2881 ggctcacgtg gctgcgcagc gcccatcgcc cgcgctggtg tagcgacaac gtgattgtgt
     2941 ttcacagtga tcgaggtgcc gacggccaac cgcacgtgtg ggcagtggag cgcggaaagg
     3001 cgccccggca gttgacgtat ggacgcaggg aagtcgaacc agattgcgtg ccagcgacgg
     3061 gcgacgtgtt gtacgtgggc tcgtccagtc gtggatccga gctgttccgg acgactctcg
     3121 tcggtggcca ggcgacgcag ttgacgcatg acggagcgtc ggctcgtttt ccgcgggtct
     3181 cgcgaaatgg cgcgtggatt gcgtttgtat cgacgcgcga gggtccgtcg gagattttca
     3241 tcatgccgag cggcggcgga ccggcgcgcc gcctcaccag tgacggcggc gagaaatctc
     3301 caccggcctg gtcctcggac ggcaagtccc tcgtctacag cgtcaaacgc ggcgagcccc
     3361 agatccgacg ctacgcggct ccggcctgct cgcttcactc ttgctgacag caaacgagcg
     3421 gtcgcattcg cttgcacgtt gaataggccc acgcagtagg cgagtcaggc gacccaacaa
     3481 agcatttgga gaagacgggc gctgatcgtc cgcttccacc ccttttcgtg cctgatgtcc
     3541 gttttccacc caaagcggac attggagccg ccgtcgacct gtcccgcatc ggcacgccct
     3601 cgaagcggcc ggtttccacg gccgcccggg cgggcaagat ggcggcatgg acttcgacac
     3661 gctggattat gcgcggatga gcgaggaggc gatggccgcg gccgatggct cggacacgct
     3721 ggaggaatgc caggcgcatc tcgaccgggc ggttcgctat gcccagctgg cgtgcctgga
     3781 acggcaccgg ccgggccggt cgaacgtcgt cgacatcgcc tcggcgcggc gcgccgctcg
     3841 ctagatcagg aggcttcgcg ctggtcgatg taccaggggc gcagcatggt gacttcgttc
     3901 agccgctctt tcttgggcgc gagtcgggcg aggtcgaacg tgtcggtgaa ctggaccccg
     3961 aacttgcccg actgcgacca gcggaccgag ccgacgaccg gcccgacgcc gacgatgtcg
     4021 atggtgatca cggtgcccgg cgagatcggc tgggcgcatt ccactagcgc ccccatcgcc
     4081 gagatgttgc gcagccggat ctcggtcacc tggccgtcga tctcggcgac cgcgcggcgc
     4141 atcaggcgct gcctcggctc gcggatgcac tggtagccgt cggcctcgac ggtgacgagg
     4201 ttggcgagct ggcgcgcctc ctcggccgcg gccggccggc cgaagatgta gccctggacc
     4261 tggctgcagc cgagttcgcg gatcaggtgc aagtcgtcgt gggtctcgac gccctcggcg
     4321 gtggtgtcca tgcccagcgt gtcggcgagc gtgacgatgg cgcggatgat cgcgctgttg
     4381 cggttggtgg tcgaggcggc gccgcgcacg aagctctggt cgatcttgat cttgtcgaac
     4441 ggcgccttct tcaaatagcc gagcgagctg tagccggtgc cgaaatcgtc gagcgccagc
     4501 cggactccga gcttcttgag gcccgcgaag gtctcgtcgg tgccctcgcc ctcggcgagg
     4561 aacacgcctt cggtgatctc gagctcgagc cgctcggagc gcaccccggt ttccttgagg
     4621 agctcggcga cgattccgac gatccccgga tcgttgaact ggatcggcga cagattgacc
     4681 gcgacccgga cctggtcggg ccagtccgcg gcctcgagga gagcggtcct gagcacccat
     4741 ttgccgattc cggggatcag cccgcattcc tcggcgagcg gaatgaactt gtcgggggag
     4801 atcgggccgc ggaccgggtg gttccagcgg accagcgcct cgaagccgca gatctcctcg
     4861 ccggcgaccc ggacgatcgg ctggtacgcg acccacagct cgccgcggtc gatcgcctgg
     4921 cggaggtcgt tctcgagcac ctggcggtcg gaaaggtcgc tgtgcatcgc cggctcgtag
     4981 aagcaatgct tgccgcggcc ggccgccttg gccgcgtaga gcgcgaggtc ggcgttgcgg
     5041 atcaggctgt cggcgcacgc acggccggga tcgccgatgg cgattccgac cgaggcgccg
     5101 atcatcaccc ggtgcccttc gatggtgtag ggcctcgaga cctgttcgat cagggtccgg
     5161 gcgagcggct ccaggaagcc gatgtcgacg gtgcccggga gcaccgcctt gaactcgtcg
     5221 ccgccgaggc ggccgacctg gccgtggtcg cccatcaccg tcttcagccg ctccgcgacc
     5281 tggcggagca gggcatcgcc gatcgggtgg ccgagcgtgt cgttgacgtt cttgaagcgg
     5341 tcgaggtcga tgaggaacag ggcgcagccc ttctgccggc tggcggcgtt gcgcagcgcc
     5401 tcgtcgaggg tctggcgcat cagcgcccgg ttgggcaggc cggtcagcga atcgaaccgc
     5461 gcgaggcgcg agatttcctg ctcggagcgg cgctgctcgg tgagatcggt gccgatcccg
     5521 cggaatccca ggaagcggcc gcgctcgtcg aagatcgggt tgccggacaa ggaccagtgc
     5581 acgtcctggt cgctggccgg ccggaccacc acgtccgcga aggggaaccg cgcggacaga
     5641 tggaagccga gcgtcttgcg ctcttcgtgg atcgagccga ccgggctggt gtcgaccgac
     5701 agcaggtccg cgaacggccg accgagcagc gcttgcggtt cgcactggaa gtcgtccgca
     5761 agctgttgcg agacatagga cagggtgccc tcggaattgg tttcccagaa ccagccgcgg
     5821 ccgctgttct cgaactcggc gacgaaggtc agcgccttgc gggcttcggc gtcgaggttg
     5881 aggcgcttgc gccctgccgc gatgatcgtc tgggcaccgg cgacgctgta ggcgacgatg
     5941 atcaggcaca aaagcgcggc gccgccgccg accaggaccg atccggtaag gacgaaggcg
     6001 ccggcggtcg tcaccagcga attggccagc gccagcgccg gcgaattgac cgcgaccatc
     6061 gccgccatgg tgatcccgcc gccgatcagc aggatcggga aggacgcgtg gccgacgtgc
     6121 gcctgcgcgc tcatcgccag cccgaccagg atccacaggc agccggcggc ggccagatag
     6181 acggcgaggg cgcggatgac ggtgtgcggg gccaggttga gccgatcgcg ccactggagg
     6241 ccgacaatcg cgacaaagtc gagcgccagc agcatcagca tcggaatgac ggggttcgcg
     6301 agcgacgtcg acagcgcccg ctccggatga agcgcgacca gcgcgatcgc ggcgatcagg
     6361 tgagtgattc cgagcatcag ggcgcaatag tcgagcgagg cgacccggat cgccgacagc
     6421 gagtgctcgt cgggcgtgtc cggggcgagg atgtcgaacc acagggcctc gcgaatcgag
     6481 gcgcggctct tggcctcgcc gggaatctcc acagcgcctg cgaaacgcgt cctacgcatc
     6541 ggcaataaac ctcacttcgg acaggaattc gtccgaaatg aaggatttac ccctcgcccg
     6601 ttaacgccac ccctaaggaa ggcggttagc ggcgcattag ctaagcttct tcgggcagga
     6661 agtcgggcac cgacagatag cgctcgccgg tgtcgtagtt gaagccgagc acccgcggag
     6721 cttctcccag ctcgggcagc ttccgggcga tcgcggcgag cgtcgctccc gacgaaatgc
     6781 cgaccagaac gccttcctcg cgggccgcgc gccgggccat ctccttggcg tcggcggggt
     6841 cgacctggat caccccgtcg agcaggccgg tgtccatgat cgcgggcacg aagccggcgc
     6901 cgatgccctg gatcgggtgc gggccgggct cgccgcccga cagcaccggc gaaagggtcg
     6961 gttcgaccgc gaacaccctc agggccggcc attcccgctt gagcacttcg gcgcaggcgg
     7021 tgatgtggcc gccggtgccg acgccggtga tcagcacgtc gatcggcgag tcgcggaagt
     7081 cggacaggat ctcgcgcgcg gtggtgcgcc ggtggacctc gaggttggcc gggttctcga
     7141 attgctgcgg catccacgcg ccgtcggtct cggcgacgat ctcctcggcg cgggcgatcg
     7201 cgcccttcat gcccttctcg cgcggagtca ggtcgaacgt cgcgccataa gcgagcatca
     7261 gccgccggcg ctcgatgctc atactgtcgg gcatcaccag gaccagccgg tagcccttga
     7321 ccgcggcgac catggcgagg ccgatgccgg tgttgcccga ggtcggctcg acgatcgtcc
     7381 cgccgggctt cagggcgccc gatgcctccg cagcctcgac catcgacaaa gcgatccggt
     7441 ccttgatcga ggcgccgggg ttcgagcgct ccgacttgat ccacacctcg gcgttgtcgc
     7501 cgaacaggcg gttgacgcgg atgtgcggcg tgttgccgat cgtctcgagg atgctggcgg
     7561 ctttcatgcg ggtttttcct ccatgacggc gaatggcagt gcggttgcgg ggggctcaaa
     7621 ggtccgggcg cgggcgagtt ccgggaacag ataggaccac aggccggcga ttcctatcgc
     7681 ggccgcgccg ccgaacagga cggcgccgac ggggccgagg atcgctgcgg cgatgcccga
     7741 ctggagctcg ccgagctcgt tcgaggccga gatcgccagg ccagagaccg ccgacacgcg
     7801 gccgcgcatg gtgtcgggcg tgtgcagctg aaccaggctc gagcgcacgt agaccgacag
     7861 catgtcgaag ccgccgagca gggcgagcgc ggcgagcgac agccacatct cccgcgacag
     7921 gccgaacagg atggtcatca ccccgaacgc ggcgacagcg aggatcatct tcacgccgac
     7981 attgtgcctc agcggccgcc acgcgaacca taaggcgacg atcgcggcgc cgaccgcggg
     8041 cgcggcgcgg agcaggccga ggccctcggt cccgacgtgc aggatgtcgc gcgcgaaaac
     8101 cggcagcatc gccgtcgctc cgccgagcag gacggcgaac aggtcgagcg tgatcgcccc
     8161 gaacaggaag cgatggtggc gcacgtagga caggccgtcg gccatttgcc ggagcggatg
     8221 cgcctcgacc atcggcggcg gcgcgatccg gcggatcggg gtcagcgcga ggatggacac
     8281 ggccatcagc aacgccgccg cccagtacgg cgcggacggg ctggccgcgt agaggaaccc
     8341 gccgagcgcc ggccc
//
LOCUS       DASTZO010000011         6369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1175899,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000011 DASTZO010000000
VERSION     DASTZO010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6369
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1175899"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..1835)
                     /locus_tag="VFU20_00250"
     CDS             complement(78..1835)
                     /locus_tag="VFU20_00250"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU5284921.1"
                     /translation="MIRVVALRKAAVAGAAGAVAWEAALRGFSLAGLPLFDIVRSLGT
                     LVFRADAPLAWWPVGLAAHMLVGVCWALVYAYFFWGRFDWPPPLQGLAFSALPAALAL
                     LIVSPQLRLMQMGERQVRLTAELIGPSLGAREIAGLLLGHVIFGLTVGALYTRPVGYR
                     ADRPPRLESRPASRRGRKPKRRRQDGDGFIFATGIECSYPTIEGGRWRRDQLDSTRHY
                     ERWSDDFELAREIGVTHLRYGPPLHLLFRGPGQYDWALVDEPMAELRDHGPEPIVDLC
                     HFGVPAWLGDFQNPDVARALGEYAGAFAGRYPWVRFYTPVNEIYVSARMSALDGAWNE
                     QRSDETAFVTAAFNLAGASVAMTDAILLARPDALFVNSESSEFAQPCCPDPEIRAIAE
                     FENERRFLPLDLIYAHEVGERMLGYLREHGVEPDYRQFLQREVPRRTVLGLDYYEWNE
                     RLIDNDGRPRALGELFGWYVIASQYHERYRRPMMHTETNRLDASDGPRWLWRQWHNVQ
                     LLRGAGVPLVGFTWYSLTDQVDWDIALAQALGKVNPVGLFDLNRDVRPVGLSYKHLIE
                     MHRDQPEYRHCPALAELLG"
     gene            complement(1848..2327)
                     /locus_tag="VFU20_00255"
     CDS             complement(1848..2327)
                     /locus_tag="VFU20_00255"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEU5284922.1"
                     /translation="MTDDRGPIHDHPSSTAKIATHPIHPMLVPFPIVCFIGAFVADIM
                     YGSNGEPGWATASNWLLGVGLGTAALAAIAGLTDFLGDSRVRRLSDALKHMVANVLAV
                     LIEAFNLLLRLGNPDFIVSTGIYLSGLVVLILLYSGWKGGELVYRHGVGVLDRDTAR"
     gene            complement(2324..3319)
                     /locus_tag="VFU20_00260"
     CDS             complement(2324..3319)
                     /locus_tag="VFU20_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5284923.1"
                     /translation="MAEVAVITGASAGIGRATAREFARRGCRVALLARGRDGLDAAAG
                     DVEELGGKALVVPTDVADAAQVERAADRVVAEFGRIDVWVNNAFAGIFSRFMDMSLEE
                     FRRVTDVTFFGQVHGTRAALKHMLPRDCGTIVLVGSALAYRGIPLQSAYCAAKHAVQG
                     FQDSVRSELLAMDSHVHLTMVQLPGVNTPQFDWIRAHVRGRPKPVGAVYQPEVAALAI
                     WKAAHSTRKQWLVGAPAYQAVLGDKLMSPLLDRYLAGTAVEGQQDDQPVEPGRKDNLF
                     EPVPGDHGAHGRFDEGARTRSPLLWASQHRAWLAAGAAIALGTTILTGRNKGGKT"
     gene            complement(3393..3797)
                     /locus_tag="VFU20_00265"
     CDS             complement(3393..3797)
                     /locus_tag="VFU20_00265"
                     /inference="COORDINATES: protein motif:HMM:NF020157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HEU5284924.1"
                     /translation="MRAGGMNWAAVLAAAVAFYALGFVIYGMIVPEETMLAWSRLTQE
                     EMASGMARMPFGALLPLATALFMAVLFNLGGVDGAAKGARWGMVIALASAIPTIWYGW
                     VYELRPFEAGLLDSAHQLLGHAVVGGILGRWR"
     gene            3896..4594
                     /gene="trmB"
                     /locus_tag="VFU20_00270"
     CDS             3896..4594
                     /gene="trmB"
                     /locus_tag="VFU20_00270"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEU5284925.1"
                     /translation="MTAHKAGDPTTLNRLYGRSSGHKLRPAQQQLLDTLLPAIAVPAD
                     GGINSRRLFGNERPLHLEIGFGSGEHLAERADLLPDHGFIGCEPFVNGVAAALAHVRD
                     RRLANVRIHRGDALDVLRRLPDGALSFVYLLHPDPWPKARHAKRRMVNDGPLDLIAAK
                     LKPGGELRLATDDPTYLAWSLMVMQRHSSRFEWLAQKPSDFLEPPGGWIETRYGAKSR
                     REGRRPYYLRYRRT"
     gene            complement(4579..5730)
                     /locus_tag="VFU20_00275"
     CDS             complement(4579..5730)
                     /locus_tag="VFU20_00275"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5284926.1"
                     /translation="MTLRLIQAAALTLLAACGSAPSSTAAQTRGERPFTATEVARFST
                     PWAMDFLPGSGVRLTGAALLTEKEGRLWLVNVNTGARQPVGGVPRVKAAGQGGLGDVV
                     VHPGFAGNRRVYLSYAEAGPNGTAGAAIGYGRLILGQGPPRLADFRVIWRQTPKVSGD
                     GHFAHRIAFARDGTMFVTSGDRQKMDPAQDLGSDLGKILRLTDEGRPVAGGPWAGRGG
                     RAAGYWSMGHRNPLGLGFAPDGRLWVSEMGPQGGDELNLILRGRNYGWPRASYGSHYG
                     GAPIPDDHRRRGFEEPKLWWTPSVSPGSLLIYTGDLFPQWKGDALLGALSGEALIRAD
                     IDGDRARKAEHWPMGARIRAVDQGPRGEVYLLEDGGRLLRLEPAAQVRR"
     gene            5774..>6369
                     /locus_tag="VFU20_00280"
     CDS             5774..>6369
                     /locus_tag="VFU20_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEU5284927.1"
                     /translation="MHSCSLLSLATAVPNNRVAQADAKAAARRAFGGKAPLFDRLASV
                     FDNAGIDQRHVVAPPDWYEEGHGWADRNAVYLQAAERLFEDAAAAAIARAGLAPDAID
                     GVVSVSTTGIATPSLEARVGPRLGLRPDVRRVPLFGLGCAGGVSGLATAARLARAEPG
                     SRWLFVTVETCSISIRLDSDDPAAVVATALFGDGAAAAV"
BASE COUNT          967 a         2280 c         2230 g          892 t
ORIGIN      
        1 tccgccgatc gccccggcga gcagcggccg ctggttcggc ccggggtcgt agccgtgcgc
       61 gccgaggaag ccggccatca gcccagcagc tccgcaagcg ccgggcagtg ccggtattcg
      121 ggctggtcgc ggtgcatctc gatcagatgc ttgtacgaca atccgaccgg ccggacgtcg
      181 cggttgaggt cgaacaggcc gaccggattg accttgccga gggcttgcgc gagcgcgatg
      241 tcccagtcga cctggtcggt caggctgtac caggtgaagc cgaccagcgg cactccggcg
      301 ccgcgcagca gctgaacatt gtgccactgc cgccatagcc agcgcggccc gtcggacgcg
      361 tcgaggcggt tggtctcggt gtgcatcatc ggccgccggt agcgctcgtg gtactggctg
      421 gcgatcacgt accagccgaa cagctcgccg agcgcccgcg gccgcccgtc attgtcgatc
      481 agccgctcgt tccactcgta ataatcgagg ccgaggaccg tccggcgcgg cacctcgcgc
      541 tggaggaact ggcggtagtc cggctcgacg ccatgctcgc gcagatagcc gagcatccgc
      601 tcgccgacct cgtgcgcgta gatgaggtcc agcggcagga accgccgctc gttctcgaac
      661 tcggcgattg cgcggatttc cgggtcggga cagcatggct gggcgaactc gctgctctcg
      721 ctgttgacga acagcgcgtc gggccgggcg aggaggatgg cgtcggtcat cgcgacgctg
      781 gccccggcga ggttgaacgc cgcggtgacg aacgctgtct cgtcgctccg ctgctcgttc
      841 caggcgccgt cgagggcgct catccgggcg ctgacgtaga tctcgttgac cggcgtgtag
      901 aagcggaccc acgggtagcg cccggcaaag gcgccggcat attcgcccag cgcgcgcgcg
      961 acgtccgggt tctggaaatc gccgagccag gccgggaccc cgaaatggca caggtcgacg
     1021 atcggctcgg gtccgtggtc gcgaagctcg gccatcggct cgtcgaccag cgcccagtcg
     1081 tattgcccgg ggcccctgaa cagcaggtgc agcggcggcc cgtagcggag gtgcgtgacc
     1141 ccgatctcgc gggcgagctc gaaatcgtcg ctccagcgct cgtagtggcg ggtcgagtcg
     1201 agctggtcgc gccgccaccg gccgccctcg atggtcgggt agctgcattc gatcccggtg
     1261 gcgaagatga acccgtcgcc gtcctgcctg cgccgcttcg gcttgcggcc gcgccgggag
     1321 gcgggccgcg attcgagccg cggcggccga tcggctcggt agccgaccgg gcgcgtgtac
     1381 agcgcgccga cggtcagccc gaagatcacg tggccgagca gcagcccggc gatctcgcgc
     1441 gcgcccagcg acggcccgat cagctcggcg gtcagccgca cctgccgttc gcccatctgc
     1501 atcagccgca gctgcggtga gacgatcagc agcgccagcg ccgccggcaa ggcgctgaac
     1561 gccaggccct gcagcggcgg cggccagtcg aaccggcccc agaagaaata ggcgtagacc
     1621 agcgcccagc agaccccgac cagcatgtgc gcggcgaggc ccacgggcca ccaggcgagc
     1681 ggcgcatcgg cgcggaagac gagcgtcccg agcgaccgga cgatgtcaaa cagcggcaat
     1741 cccgcgaggc tgaacccgcg cagcgccgct tcccaggcga ccgctccggc cgcgcccgcg
     1801 accgccgcct tgcgcagcgc gacgacgcgg atcaaccggc gcgctcccta gcgggcggtg
     1861 tcgcggtcga gaacgccgac gccgtggcga tagacgagct cgccgccctt ccagccgctg
     1921 tagagcagga tcaacacgac cagacccgac agatagatgc cggtcgagac gatgaagtcg
     1981 gggttgccga gcctgagcag taggttgaac gcctcgatca gcaccgcgag cacgttggcg
     2041 accatgtgct tgagcgcgtc ggacagtctg cgcacgcgcg aatcgccgag aaaatcggtc
     2101 aggccggcga tcgccgcgag cgcggcggtt ccgaggccga cgccgagcag ccagttggag
     2161 gcggtcgccc agcccggctc gccgttcgag ccgtacatga tgtcggcgac gaacgcgccg
     2221 atgaagcaga cgatcgggaa cggcacaagc atcgggtgga tcgggtgggt cgcgatcttc
     2281 gccgtgctgc tcggatgatc gtggattgga cctcggtcat cggtcatgtc ttgcctcctt
     2341 tgttccgccc cgtgagaatc gtcgtgccga gcgcgatcgc cgcgccggcg gccagccagg
     2401 cgcggtgctg gctcgcccac agcagcggcg atcgagtccg cgccccttca tcgaagcggc
     2461 cgtgagcgcc gtggtcgccg ggcaccggct cgaagagatt gtccttgcgg cccggctcga
     2521 ccggctggtc gtcctgctgg ccctcgaccg cggtcccggc gagatagcgg tcgagcagcg
     2581 gcgacatcag cttgtctccc aagaccgcct ggtaagcggg cgctccgacc agccattgct
     2641 tgcgcgtcga atgggcggcc ttccagatcg ccagcgccgc gacctcgggc tggtagaccg
     2701 ctccgaccgg cttgggccgg ccgcgcacat gggcgcggat ccagtcgaac tggggcgtgt
     2761 tgacgcccgg cagctggacc atcgtcagat ggacgtggct gtccatcgcc agcaattcgc
     2821 tgcgcaccga atcctggaac ccctggaccg cgtgcttggc ggcgcaatag gccgactgca
     2881 gcgggattcc gcggtaagcg agcgccgagc cgaccaggac gatggttccg caatcgcgcg
     2941 gtagcatgtg cttcaaggcc gcgcgggtgc cgtggacctg gccgaaaaaa gtgacgtcgg
     3001 tgacccgccg aaattcctcg aggctcatgt ccatgaaccg cgagaaaatg ccggcgaaag
     3061 cgttgttcac ccagacgtcg atgcgcccga actcggcgac gactcggtcg gccgcgcgct
     3121 cgacctgcgc ggcgtcggcg acgtcggtcg ggacgaccag cgccttgccg ccgagctcct
     3181 cgacgtcccc cgcggcggca tcaaggccgt cgcggccgcg ggcgagcagc gccacccggc
     3241 aaccgcgccg ggcgaactca cgggcagtcg cacggccaat gcccgccgag gctccggtga
     3301 tgactgcaac ttccgccatg gaggcctcca gggaccttca acggcattgg acgccacagc
     3361 cgcggcgaag ttcccgacgg gctatgccgg actcagcgcc agcggccgag gatccccccg
     3421 accacggcgt gcccgagcag ctggtgagcg ctgtcgagca gccccgcttc gaagggccgg
     3481 agctcataaa cccagccgta ccagatcgtc gggatagccg aggcgagagc gatcaccatc
     3541 ccccagcggg cgcccttggc cgcgccgtcg acgccgccaa ggttgaacag gacggccatg
     3601 aacagcgcgg tcgccagcgg cagcagcgct ccgaacggca tgcgcgccat tccactggcc
     3661 atctcttcct gcgtgagccg cgaccaggcc agcatcgtct cttccgggac gatcattccg
     3721 tagatcacga acccgagtgc gtagaaagcc acggccgcgg ccagcacggc ggcccaattc
     3781 atccctccag cgcgcatgat atcctcccct gttacgcaac cgggacatat catcgccgag
     3841 cgggctaatc caacggcgtt gcgcgggccg cgggcggcgc ttagcgagcc ttgcgatgac
     3901 cgctcacaag gccggcgacc cgaccaccct caaccggctg tacggccggt cgagcggcca
     3961 taagctgcgc ccggcgcaac agcagctgct cgacaccttg ctgccggcga tcgcggtgcc
     4021 tgcggacggc gggatcaatt cgcggcgcct gttcgggaac gagcgccccc tgcacctcga
     4081 gatcggcttc ggctcgggcg agcatctcgc cgagcgcgcc gacctgctgc ccgaccacgg
     4141 cttcatcggc tgcgagccgt tcgtcaatgg ggtcgcggcg gcgctcgccc acgtccgcga
     4201 ccgccgcctc gccaacgtcc gcatccaccg cggcgacgcg ctcgacgtgc ttcggcgcct
     4261 gcccgacgga gcgttgagct tcgtctatct gctccacccg gacccgtggc ccaaggcgcg
     4321 gcacgccaag cggcggatgg tcaacgacgg gccgctcgac ctgatcgccg cgaagctgaa
     4381 gcccggcggc gagctccggc tggcgaccga cgacccgacc tacctcgcct ggtcgctgat
     4441 ggtgatgcag cgccactcca gccggttcga atggctggct caaaagccgt cggacttcct
     4501 cgagccgccg ggcggctgga tcgaaacgcg ctacggcgcg aagtcgcggc gcgagggccg
     4561 ccggccctat tatttgcgct accgccggac ttgagccgcc ggctcgagcc tcagcaagcg
     4621 gccgccgtcc tcgagcagat agacttcgcc gcgcgggccc tggtcgacgg cccggatccg
     4681 cgcgcccatc ggccagtgtt ccgctttgcg cgcccgatcg ccgtcgatgt cggcccggat
     4741 cagcgcctcg ccggacaggg cgccgagcaa tgcgtcgccc ttccattgcg ggaacaggtc
     4801 gccggtgtag atcagcaggc tgccggggga gaccgacggg gtccaccaca atttgggctc
     4861 ctcgaagccg cggcggcgat gatcgtcggg gatcggcgcg ccaccgtagt ggctgccata
     4921 ggacgcgcgc ggccagccgt aattgcgccc gcgcaggatg aggttgagct cgtcgccgcc
     4981 ctgcgggccc atctcgctga cccacagccg cccgtcgggg gcgaagccga ggccgagcgg
     5041 gttgcggtgg cccatcgacc aatagcccgc cgcgcggccg ccgcggcccg cccacgggcc
     5101 gccggcgacc ggccggccct cgtcggtcag gcgcaggatc ttgccgaggt cgctcccgag
     5161 gtcctgcgcc gggtccatct tctgccggtc gccggacgtg acgaacatcg tgccgtcgcg
     5221 ggcgaaggcg atgcggtggg cgaaatggcc gtcgccgctg accttggggg tctgccgcca
     5281 gatgacccgg aagtcagcca gccgcggcgg tccctgcccg aggatcagcc ggccgtaacc
     5341 gatcgcggct ccggcggtgc cgttcgggcc cgcctcggcg tagctcagat agacccgccg
     5401 gttgcccgcg aaccccgggt ggacgacgac gtcgccgagc ccgccctggc cggcggcctt
     5461 gacccgcggg actccgccga ccggctgccg ggcgccggtg ttgacgttga ccagccacag
     5521 ccggccctcc ttttcggtga gcagcgccgc gccggtcagc cggacgccgc tcccgggcag
     5581 gaaatccatc gcccaggggg tcgagaagcg cgcgacctcg gtcgccgtga acgggcgctc
     5641 gccgcgggtc tgggcggcag tcgacgaagg cgccgacccg caggcggcca gcagggtcag
     5701 cgccgcggcc tgtattaagc gaagggtcat tcgatacagt ccctagcttg cgcacccgtg
     5761 tataggtgcc gacatgcact cgtgcagcct actctcattg gcgacggcgg ttccaaacaa
     5821 ccgggtcgcg caagccgatg ccaaggcggc cgcccgccgg gcgttcggcg gcaaggcgcc
     5881 gctgttcgac cggctggcaa gcgtgttcga caatgccggc atcgaccagc gacacgtggt
     5941 cgcgccgccc gactggtacg aagaaggcca cggctgggcg gaccgcaacg cggtctacct
     6001 gcaggccgcc gagcgtctgt tcgaggacgc cgcggccgcc gcgatcgcca gggcggggct
     6061 cgctcccgac gccatcgacg gcgtcgtcag cgtctcgacc accgggatcg cgaccccgag
     6121 cctcgaggcg cgggtcgggc cgcggttggg cttgaggccg gatgtccgcc gggtgccgct
     6181 gttcggactc ggctgcgcgg gcggggtcag cgggctcgcg accgcggccc ggctcgctcg
     6241 cgccgagccc ggcagccgct ggctgttcgt gaccgtcgag acttgctcga tctcgatccg
     6301 gcttgacagc gacgacccgg ccgcggtggt cgccaccgct ctgttcggcg acggcgccgc
     6361 cgcggcggt
//
LOCUS       DASTZO010000012         3364 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_705963,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000012 DASTZO010000000
VERSION     DASTZO010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705963"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="VFU20_00285"
     CDS             complement(<1..804)
                     /locus_tag="VFU20_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU5284928.1"
                     /translation="MTGATKRAVGAERALLSSRAALASVALALVLLAAKAWAAVETNS
                     TAMLGSLADTALDLVASLATLAGVVIAARPADRDHRFGHGKAEALVALGQVALIGVSA
                     LWIAWRALDRLINGGATAEMELGIGVSLFAMAATVALLAYQRRVIARTGSVAIRTDHV
                     HYQSDLLLNSAVIAALVLDQWAGLRGADALFGIAIAVWLLWGAFRSSGQAVNQLMDRE
                     WPEEERARFLATAAEYPELAGLHDLRTRTSGTHHFAQFHVWVPADWTVQE"
     gene            complement(801..1379)
                     /gene="pdxH"
                     /locus_tag="VFU20_00290"
     CDS             complement(801..1379)
                     /gene="pdxH"
                     /locus_tag="VFU20_00290"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HEU5284929.1"
                     /translation="MTGDPFELFDRWFAEARAAEPNDPEAMALATADADGRPSLRMVL
                     LKAHGPDGFTFYTNLGSRKGAELAANPNVALLFHWKSLRRQVRIEGRVEGVPAEEADA
                     YFATRSRDSQIGAWASDQSRPLDRRETFEARCDEVARRFEGQSVPRPPHWAGYRVVPA
                     CIEFWTDRPHRLHERRLFTRNGGGWSEGLLYP"
     gene            1460..2425
                     /locus_tag="VFU20_00295"
     CDS             1460..2425
                     /locus_tag="VFU20_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEU5284930.1"
                     /translation="MDLYQRLGIKRGASDEEVKKAYRSLAKQLHPDRNKDNPNAAKRF
                     AEVTHAYDLLSDKDKRARYDRGEIDEDGNPKMPFGAGFGGYSPGGGAGARPGPGGGFE
                     GFPGGFDAGGAADLSDLFEGLFGAATAGRGRRQSGPTGFRQRARAPQKGADVAYRLKV
                     PFVDAATLAPQRITLADGKTIDLKLPKGLEDGTRIRLSGKGEPGPAGPGDAIVTVSIA
                     PHPFYTREGNHIRLSLPVTLKEAVLGAKVKVPTPDGPVMLTIPKGTSSGKVLRLKGRG
                     FTDKAGKRGDQLVTVAVDLPAGDPELDAFAQRWKGGGNPRAPLGV"
     gene            2439..>3364
                     /locus_tag="VFU20_00300"
     CDS             2439..>3364
                     /locus_tag="VFU20_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5284931.1"
                     /translation="MKDVSPQSPEARRKRLASMEAAFGHEVVEKLKPRHYAYEVLKRV
                     AVGVYNDGFIHAGNLAYLALLALFPFFILAAAIAHILGQTESGAMTVATVLSQLPPDV
                     AGTLRGPVSEVLTARTGPLLWLGAIVGAWTAASFIETIRDILRRAYGVNFCAPFWEYR
                     LGSIGMIFAAVLLLMFTFALSVMLSSLQHAISAWVPFAEDVVSTLAIYRVVPAIALFG
                     TIYALFFALTPARYRKSGCRKWPGALLITLWWLATVELLPETISLVGGYDLTYGSLAS
                     VMIVLIFFFMIGLGVVAGAELNAALAEVRPTAL"
BASE COUNT          512 a         1192 c         1176 g          484 t
ORIGIN      
        1 ctcctggact gtccagtcgg ccggcaccca gacgtggaac tgggcgaagt gatgggtgcc
       61 gctggtccgc gtgcgcaggt cgtggaggcc ggcgagctcg ggatattcgg ccgccgtggc
      121 gaggaagcgg gcgcgctcct cctccggcca ttcgcggtcc atcagctggt tgaccgcttg
      181 gccggacgag cggaacgccc cccacagcag ccacacagcg attgcgatcc cgaacaaagc
      241 gtcggcgccg cgcaggcccg cccactggtc gagcacgagc gcggcgatca ccgccgaatt
      301 gagcagcagg tccgactggt aatggacatg gtcggtgcgg atcgcgaccg agccggtgcg
      361 ggcgatcacc cgccgctggt aggcaaggag cgcgacggtc gcggccatcg cgaacagcga
      421 cacgccgatt cccagctcca tctcggcggt cgccccgccg ttgatcagcc ggtcgagcgc
      481 gcgccacgcg atccacagcg ccgagacgcc gatcagcgcc acctggccca gggcgaccag
      541 ggcctcggcc ttgccgtgcc cgaagcggtg gtcgcggtcg gccggccggg cggcgatgac
      601 gactccggcg agcgtcgcga ggctggcgac gaggtccagc gcggtgtcgg cgagcgagcc
      661 gagcatcgcg gtcgaattgg tttcgaccgc cgcccaggcc ttggcggcca gaagcaccag
      721 cgccaaggcg acgctggcca gcgccgcgcg cgacgacagc agcgcccgct cggcgccgac
      781 cgcccgtttc gtcgccccgg tcacgggtag agcagccctt cgctccagcc gccgccgttc
      841 ctggtgaaca ggcgccgctc gtggagccgg tgcggccggt cggtccagaa ctcgatgcac
      901 gccgggacga cccggtagcc tgcccagtgc ggcggccgcg gcacgctctg gccctcgaag
      961 cgtcgggcga cctcgtcgca gcgcgcctcg aacgtctcgc ggcggtcgag cggccgcgac
     1021 tggtccgacg cccaggcgcc gatctgcgaa tctcgggacc gagtcgcgaa ataggcatcc
     1081 gcttcctcgg ccggcacgcc ttccacgcgc ccctcgatcc gcacctggcg gcgcagcgac
     1141 ttccagtgga acagcaaggc gacgttgggg ttggcggcga gctcggcgcc cttgcggctg
     1201 ccgaggttgg tgtagaaggt gaagccgtcg ggcccgtgcg ccttcagcag caccatccga
     1261 agcgacggac ggccatcggc gtccgcagtc gccagcgcca tcgcctcggg gtcgttgggc
     1321 tcggcggcgc gagcctccgc gaaccatcgg tcgaacagct cgaaggggtc gccggtcatc
     1381 ctggcctcgg cttaggcttg gaccgagccg cgcggcaacc catctggaaa catcgggacc
     1441 gatacggcat ggggaggcga tggacctgta ccagaggctc gggatcaagc gcggggcgag
     1501 cgacgaggag gtcaagaagg cctatcgcag cctcgccaag cagctccacc ccgaccgcaa
     1561 caaggacaat cccaacgccg ccaagcgctt cgccgaggtg acccacgcct acgatttgct
     1621 gtccgacaag gacaagcgcg cccgctacga ccgcggcgag atcgacgagg acggcaatcc
     1681 gaagatgccg ttcggggccg gcttcggcgg ctattcgccc ggcggcgggg cgggagcgcg
     1741 gcccgggcct ggcggcggct tcgagggctt cccgggcggc ttcgacgccg gcggggcggc
     1801 cgacttgagc gacctgttcg aagggctgtt cggagccgcc accgcgggcc gcggtcggcg
     1861 gcagtcgggg ccgacgggct tccggcagcg cgcccgggcg ccgcagaaag gcgccgacgt
     1921 cgcctaccgg ctcaaggtgc cgttcgtcga cgcggcgacg ctcgcgccgc agcggatcac
     1981 gctcgccgac ggcaagacca tcgatctcaa gctgcccaag ggcctcgagg acggcacccg
     2041 gatccggctg tcgggcaagg gcgagcccgg gcccgccggc cccggcgacg cgatcgtcac
     2101 tgtcagcatc gcgccgcacc ccttctacac gcgcgaaggc aaccatatcc ggctgagcct
     2161 gccggtgacc ctcaaggagg cggtgctcgg cgccaaggtc aaggtgccga cccccgacgg
     2221 gccggtgatg ctgaccatcc ccaaggggac cagctcgggc aaggtgctgc gattgaaggg
     2281 ccgcggcttc accgacaagg ccggcaagcg cggcgaccag ctggtcacgg tcgcggtcga
     2341 cctgcccgcc ggcgatcccg agctcgatgc gttcgcgcag cgctggaaag gcggcggcaa
     2401 cccgcgcgcg ccgctcggag tttagcttcg gccgaataat gaaagacgtc tcgccccagt
     2461 caccggaagc ccggcgcaag cgccttgcaa gcatggaggc ggcattcggc cacgaggtgg
     2521 tcgagaagct caagccgcgc cactatgcgt acgaggtgct caagcgcgtc gccgtcgggg
     2581 tctacaacga cggcttcatc cacgccggca acctcgccta cctcgcgctg ctggcgctgt
     2641 tccccttctt catcctcgcc gctgcgatcg cccatatcct cggccagacc gagagcggcg
     2701 cgatgacggt ggcgacggtg ctcagccaat tgccgccgga cgtcgcgggg accttgcggg
     2761 gaccggtcag cgaggtgctg accgcgcgca ccgggccgct gctgtggctc ggagcgatcg
     2821 tcggcgcatg gaccgcggcg agcttcatcg agaccatccg cgacattctg cgccgcgcct
     2881 acggggtcaa tttctgcgct ccgttctggg agtatcgcct gggctcgatc gggatgatct
     2941 tcgccgcggt gctgctgttg atgttcacct tcgcgctcag cgtcatgctg agctcgctcc
     3001 agcacgcgat ctccgcctgg gtaccgttcg ccgaggacgt cgtcagcacc ttggccatct
     3061 accgcgtcgt gccggcgatc gctctgttcg ggaccatcta cgccctgttc ttcgcgctga
     3121 cccccgcgcg ctaccgaaag agcggctgcc gcaagtggcc gggcgctctc ctgatcactt
     3181 tgtggtggct ggcgacggtc gagctcttgc ccgagaccat cagcctggtc ggcggctacg
     3241 acctcaccta cggcagcctc gccagcgtca tgatcgtgct cattttcttc ttcatgatcg
     3301 gcctcggggt cgtcgccgga gccgaattga acgcggcgct ggcggaagtg cggccgacgg
     3361 cgct
//
LOCUS       DASTZO010000013         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_862615,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000013 DASTZO010000000
VERSION     DASTZO010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862615"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="VFU20_00305"
     CDS             complement(<1..713)
                     /locus_tag="VFU20_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5284932.1"
                     /translation="MTAKGTTLGGRREQLEGFAFVAPYLFLYLFLLIYPLLRGILLSF
                     RRADPFEDGPFVGFENYARLAVDPVFHQAMVNTFVVTLMIVPVLTVIALALALALNLA
                     TRGAAVFRGIFFSSSVLSVTIVTLIWRFVLAPDAGLLGELATALGWEPLPFLSHPDLV
                     LPALAITTIWWSLGLPMMLFLAGLQQIPEDMYEAAALDRANRWTTFWRVTLPSLRRTL
                     ILVVMLQTAAQLQLFGQAQL"
     gene            complement(710..1807)
                     /locus_tag="VFU20_00310"
     CDS             complement(710..1807)
                     /locus_tag="VFU20_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5284933.1"
                     /translation="MASLELSAIRKAFGSTPVLDDVTLTLEAREFIAFLGPSGSGKST
                     LLRIIAGLECADSGEIRLEGRRIDGLPPGERGVAMVFQHYALYPHMTVRENMAFGLRN
                     ARVPKDEIESRVGEAARVLEIADHLDKKPGQLSGGQRQRVAIGRAIVKQPKLFLLDEP
                     LSNLDAALRMRTRVELAQLRQKVDAAVIMVTHDQAEAMTLADRIVVMNDRTIQQVGPP
                     MEIYERPANVFVAQFVGSPAMTLLPAEPVEGDGERLRLKLGDGAEVETSVPSSAARSN
                     GALRLGLRPENVRVVEGGQATTRAKVELVERLGERTLVYGRLSNGQAITAEDEGNSRV
                     KIGDEVGIAIDGAAAHVFGDDGASHHSWARP"
     gene            complement(1944..>3125)
                     /locus_tag="VFU20_00315"
     CDS             complement(1944..>3125)
                     /locus_tag="VFU20_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5284934.1"
                     /translation="PAVVAAAVTTFAVWLTVGPEPRLSHALINSVAVLIIACPCALGL
                     ATPMSIMVGTGRGASAGVLVKNAEALELMEKIDTLVVDKTGTLTLGKPKLIAVAPATG
                     FEANEVLRLGAALERGSEHPLAAAIVEGAVERGIDLPASSDFESHTGKGVTGKVDGRD
                     VALGNAALMAGLGLDVSTLQERADEHRSEGQGVMFVAVDARLAGLVVVADPIKDSAVQ
                     AIAELHRQGIRIVMLTGDNQRTAQAVARRVGIDEVMAEVLPGHKKDKVEALRREGRRV
                     AMAGDGINDAPALAAADVGIAMGTGTDVAMESAAVTLIKGDLNGIVRARALSRATMRN
                     IRQNLFFSFVFNAAGVPIAAGVLYPWFRILLSPIIAGAAMAFSSVAVIGNSLRLRSVR
                     L"
BASE COUNT          494 a         1094 c         1064 g          473 t
ORIGIN      
        1 agctgggcct ggccgaacaa ttgcagctgg gccgcggtct ggagcatcac gaccaggatc
       61 aaagtgcgcc ggagcgaggg cagggtcacc cgccagaagg tcgtccagcg gttcgcccgg
      121 tcgagcgccg ccgcctcgta catgtcctcg gggatctgct gcagcccggc gaggaacagc
      181 atcatcggca gcccgagcga ccaccagatg gtggtgatgg caagcgccgg cagcaccagg
      241 tcggggtggc tgaggaacgg cagcggctcc cagccgagcg cggtcgccag ttcgcccagc
      301 agccctgcgt ccggggccag cacgaagcgc cagatcaggg tgacgatcgt caccgacagg
      361 accgaggagg aaaagaagat tccgcggaag acggctgctc cgcgggtcgc caggttgagc
      421 gccaaggcga gcgccagcgc gatgacggtc agcaccggca cgatcatcag cgtgaccacg
      481 aatgtgttga ccatcgcctg gtggaacacc gggtcgacgg ccagccgggc gtaattctcg
      541 aatcccacga acggcccgtc ctcgaacggg tcggcgcggc ggaagctcag caggattccg
      601 cgcagcagcg ggtaaattag caggaacaga tagaggaaca ggtagggcgc gacgaaggcg
      661 aacccctcga gctgctcgcg ccgcccgccg agcgtggtgc cctttgccgt caaggccgcg
      721 cccagctgtg gtggctggcg ccgtcgtcgc cgaacacgtg cgccgcggcg ccgtcgatcg
      781 cgattccgac ctcgtcgccg atcttcaccc ggctgttgcc ctcgtcctcg gcggtgatcg
      841 cctgcccgtt cgacaggcgg ccgtagacca gggtgcgctc gccgagccgc tcgaccagct
      901 cgaccttggc ccgcgtcgtc gcctggccgc cctcgaccac ccgcacgttc tccgggcgca
      961 agccgagccg gagcgctccg ttcgagcgcg ccgcggagct cggcaccgac gtctcgacct
     1021 cggccccgtc gccgagcttc agcctcagcc gctcgccatc gccttcgacc ggctcggcgg
     1081 ggagcagggt catcgccggc gacccgacga actgagcgac gaagacgttg gccggccgct
     1141 cgtagatttc catcggcggg ccgacctgct ggatggtgcg gtcgttcatg acgacgatcc
     1201 ggtcggcgag cgtcatcgcc tccgcctggt cgtgggtgac catgatcacc gccgcgtcga
     1261 ccttctgccg aagctgggcg agctcgaccc gggtgcgcat tcggagcgct gcgtcgaggt
     1321 tggacaaggg ctcgtcgagc aggaacaatt tgggctgctt gacgatcgcc cggccgatcg
     1381 cgacccgctg gcgctggccg cccgaaagct gccccggttt cttgtcgaga tggtcggcga
     1441 tctcgagcac ccgcgccgct tcgccgaccc ggctctcgat ctcgtccttc ggcacccggg
     1501 cgttgcgcag cccgaacgcc atattctccc ggaccgtcat gtgcgggtag agcgcgtaat
     1561 gctggaacac catcgccact ccgcgctcgc ccggcggaag cccgtcgatc cggcggccct
     1621 cgagccggat ctcgccggaa tcggcgcact cgagtccggc gatgatccga agcaaggtcg
     1681 acttgcccga ccccgagggg ccgaggaagg cgatgaactc gcgggcttcg agggtgaggg
     1741 ttacgtcgtc gagaacggga gttgagccga acgctttacg gattgcgcta agctcaagac
     1801 ttgccactcg aatctccccg tgcgccggca ctctacaaga acaggcgcgc gccgcaacgg
     1861 gggtcgggca tccgcggcgg acaggccgtt ggcggcggat gacgaagtcg gttggggatg
     1921 ggcgtgcggg ggcccgtggc gccttacaac cgcaccgacc tcaagcgcag cgaattgccg
     1981 atcaccgcga ccgagctgaa cgccatcgcc gccccggcga tgatcggcga gagcaggatc
     2041 cggaaccatg gatagagcac accggccgcg atcgggactc cggcagcgtt gaagacgaac
     2101 gagaagaaca gattctgtcg gatgttgcgc atcgtcgccc ggctcagcgc ccgggcgcgg
     2161 acgattccgt tgaggtctcc cttgatcagg gtgacggccg cgctttccat ggcgacgtcg
     2221 gtcccggtgc ccatggcgat gccgacgtcg gcggcggcga gcgccggggc gtcgttgatc
     2281 ccgtccccgg ccattgcgac ccggcggcct tccctgcgca gcgcctcgac cttgtccttc
     2341 ttatgcccgg gcagcacctc ggccatcacc tcatcgattc ccacccttcg ggccacggct
     2401 tgcgcggtcc gctggttgtc gccggtcagc atgacgatcc ggatcccctg gcggtgcagc
     2461 tcggcgatgg cctggacggc gctgtccttg atcgggtccg cgaccacgac cagcccggcg
     2521 agccgagcgt cgaccgcgac gaacatcacg ccctgtccct cggagcgatg ctcgtcggcc
     2581 cgctcctgaa gggtcgagac gtcgagcccg agcccggcca tcagcgcggc gttgccgagc
     2641 gcgacgtcgc ggccgtcgac tttgccggtc acgcccttgc cggtgtggga ctcaaagtcg
     2701 ctggacgccg ggagatcgat gccgcgttcc acggcgcctt cgacgatggc cgccgcgagc
     2761 gggtgctcgc tgccccgttc gagagccgct ccgagacgca gcacctcgtt cgcctcgaag
     2821 ccggtcgccg gcgcgaccgc gatcagcttc ggcttgccca gagtcagcgt gccggtcttg
     2881 tcgacgacca aggtgtcgat cttttccatc agctccagcg cttcggcatt cttgaccagc
     2941 actccggcgc tcgcgccgcg gccggtcccg accatgatcg acatcggggt cgccagcccg
     3001 agcgcgcagg ggcaagcgat gatcagcacc gccactgagt tgatcagcgc atggctgagg
     3061 cgcggctcgg gcccgaccgt cagccacacg gcgaacgtcg tgaccgcggc cgcgaccacc
     3121 gccgg
//
LOCUS       DASTZO010000014         5142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1254408,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000014 DASTZO010000000
VERSION     DASTZO010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5142
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254408"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..613)
                     /locus_tag="VFU20_00320"
     CDS             complement(<1..613)
                     /locus_tag="VFU20_00320"
                     /inference="COORDINATES: protein motif:HMM:NF015297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEU5284935.1"
                     /translation="MKAHPRSWLFVPADSEKKIARALDSEADALIFDLEDSVAPARKK
                     AAREILKALAPRSGGPHWWVRINPLGSAHLKADLGLFASADFEGVVLPKAESGADVAQ
                     LAHRTGHLPVHAIVTETPASLFGMLSYRDPKSSLAAMSWGAEDLSAALGASSKYGADG
                     ELSFTYKLARSLCLVAASAAGVQPVDGVFADFKDEEGLRSESEA"
     gene            complement(610..1062)
                     /locus_tag="VFU20_00325"
     CDS             complement(610..1062)
                     /locus_tag="VFU20_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU5284936.1"
                     /translation="MPGRYFDEWQVGDTIAHSVTRTVTETDNVLICALTHNQQPLHLD
                     HEAAARSEFGKPLVNSVYTFGLMVGVSVADTTLGTLVANLGYDKLVFPNPVFAGDTLR
                     SETECLEVRASKSRPNAGIVTWAHRSFNQRDELVCECTRTALLLKKPA"
     gene            complement(1073..3400)
                     /locus_tag="VFU20_00330"
     CDS             complement(1073..3400)
                     /locus_tag="VFU20_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU5284937.1"
                     /translation="MLKPATTDLDALRIIEERLRWLSAWTIHHANHVRPSPDGLKVGG
                     HQASCTSMTAIMAALYFHALGPNDRVAVKPHAGPVLHAIHYLLGSQSLESLENFRGFG
                     GMQSYPSRTKDRIPVDFSTGSVGLGVAVTAFASLVQDYLSAHGWLAEADRGRFVALVG
                     DAELDEGNVYEALIEAYKHDIRNVWWIVDYNRQSLDAVSADRMFERFDEIFKSCGWRV
                     VELRWGKRLAAALDKHPAMARWLAGLPNAELSALHYQGGAAWRARIEADLGKSAAAFL
                     KSHDDDALAALFTDLGGHCMGTLVEAFDAARDDVPTLFIAWTIKGYGLPFAGHKDNHA
                     GLMNPTQMAALRESMGVAEGREWEPLEAAGANARPAVEALLERCPVAREKRSRPFGSI
                     EVPHIPTPAGDEQSTQAAFGRILNDLSRSGGPLADRIVTTSPDVTVSTNLGAWVNQRG
                     LFRRSEAADVFAKAKIASAQKWAATSTGQHIELGIAENNLFLMLAAAGLAGDLFGTRL
                     VPVGTLYDPFIARGLDALNYACYQDSRFILVATPSGITLGPEGGAHQSINPPLIALGQ
                     PGLRHYEPAFADELAAMLEQAFRLIDDPAGESSYFRLSTRSIAQPERADDGWRSGALD
                     GGYWLRPPGPKAEAAIVAMGAVMPEALAAWDELRSDLPGLGLLAVTSPDLLHRGWTRA
                     QAGRWAGKREPSHVETLLSGLAPGAGLVTIADAAPAALSWLGGVLGQRVAPLGVDRFG
                     QTGSLPDLYAAYRLDQDAITQGVAELLLAREATNA"
     gene            complement(3443..4417)
                     /locus_tag="VFU20_00335"
     CDS             complement(3443..4417)
                     /locus_tag="VFU20_00335"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEU5284938.1"
                     /translation="MSSAADPGQIRDLLGRGDSYVRDGDDRAAMSFYQAALGAARGVR
                     LVDSETMADLRRAQAFVQQRALEFEQALDRVVQGAEPASPIHRLRLTHAMDMLKGKRS
                     IYPQQPSVLYYPYLAQRQFWERDEFDWAAELEAQAPVIRAELEALLADGADFRPYIVA
                     EEDRPHRDFHGLLGDESWTALYLWKDGAVVEENARRCPKTMAALDKVPISRIGRRTPA
                     VLFSRLLPGAHIPPHHGMLNSRLICHLPLIVPPGGWLRVGNETREWREGELLVFDDSL
                     EHEARNPSGETRIILLFDVWKPELGEDERAAVSDIFDAIDAFSGASDD"
     gene            complement(4414..4788)
                     /locus_tag="VFU20_00340"
     CDS             complement(4414..4788)
                     /locus_tag="VFU20_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5284939.1"
                     /translation="MKDPSNPVCRTISTLLSRIGDKWTVLVVVTLGDGPRRFNELRRE
                     IPSVSQRMLTLTLRNLERDGIVNRTVTPSIPPRVDYELTPLGHSLLRPITALTQWALE
                     NVEEIHSAQARFDAAQDKAEAA"
     gene            4924..>5142
                     /locus_tag="VFU20_00345"
     CDS             4924..>5142
                     /locus_tag="VFU20_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU5284940.1"
                     /translation="MTILHIDSSINGDNSVSRILTRSIVDQLRAANPRAAIISRDLAS
                     EPLPHLTLDAFADSSSLDEFLAAGSVVIG"
BASE COUNT          762 a         1747 c         1821 g          812 t
ORIGIN      
        1 cggcctcgga ttcggaccgc agcccctcct cgtccttgaa gtcggcgaac accccgtcga
       61 ccggctgcac tccggccgcc gaggccgcga ccaggcacag ggaacgggcg agcttgtagg
      121 tgaagctgag ctcgccgtcg gcgccatatt tggacgacgc cccgagcgcc gccgacaaat
      181 cctcggcgcc ccagctcatc gccgccagcg acgatttggg gtcgcggtag gacagcatgc
      241 cgaacaggct cgccggggtt tcggtgacga tcgcgtggac cggcagatgg ccggtgcggt
      301 gggcgagctg ggcgacgtcg gcgccgcttt ccgccttggg caggacgacc ccttcgaagt
      361 cggcggaggc gaacagcccg aggtccgcct tcaggtgcgc gcttccgagc gggttgatcc
      421 gaacccacca gtgcgggccg ccggagcgcg gtgcaagcgc cttcaggatt tcgcgcgcgg
      481 ctttcttccg ggccggcgcg acgctgtcct cgaggtcgaa gatcagggcg tcggcctcgc
      541 tgtccagcgc gcgcgcgatc ttcttctcgc tgtcggcggg gacgaacagc cagctgcgcg
      601 ggtgggcctt catgccggct ttttcagaag cagcgcggtg cgcgtgcact cgcacaccaa
      661 ttcgtcgcgc tgattgaagc tgcggtgcgc ccaggtgacg atgcccgcgt tgggccgcga
      721 cttgctcgcc cgcacctcga ggcattcggt ctcgcttcgg agcgtgtcgc cggcgaacac
      781 cgggttgggg aagacgagct tgtcgtagcc gaggttggcg accagcgtcc cgagcgtggt
      841 gtcggcgacg ctgactccga ccatgagccc gaaggtgtag accgagttga ccagcggctt
      901 gccgaactcg gaccgggcgg cggcctcgtg gtcgaggtgc agcggctgct ggttgtgggt
      961 cagcgcgcag atcagcacat tgtcggtttc ggtgacggtt cgggtgaccg agtgggcgat
     1021 cgtgtcgccg acctgccact cgtcgaagta gcgtcctggc atggccggct ccctaagcat
     1081 ttgtcgcttc gcgcgccagc agcagttcgg cgaccccctg ggtgatcgca tcctggtcga
     1141 gccggtaggc ggcgtagagg tcgggcaggc tgccggtctg gccgaagcgg tcgaccccga
     1201 gcggcgcgac gcgctgcccg agcacgccgc cgagccacga cagtgcggcc ggggcggcgt
     1261 cggcgatggt caccagcccc gcgcccggcg ccagcccgga cagcaatgtc tcgacatggc
     1321 tcggctcgcg cttgccggcc cagcgcccgg cctgcgcccg ggtccagccg cggtggagga
     1381 ggtcgggcga ggtgaccgcg agcagcccga gccccggcag gtcggagcgc agctcgtccc
     1441 acgcggcgag cgcctcgggc atcaccgcgc ccatcgcgac gatcgccgcc tcggccttcg
     1501 gcccgggcgg ccgaagccag tagccgccgt cgagcgcgcc cgagcgccag ccgtcgtcgg
     1561 cgcgctcggg ctgggcgatc gagcgcgtcg acagccggaa ataactgctc tcgccggcgg
     1621 ggtcgtcgat cagccggaac gcctgctcga gcatcgccgc gagctcgtcg gcgaatgccg
     1681 gctcgtagtg gcgcaggccc ggctggccca atgcgatcag cggcgggttg atcgactggt
     1741 gggcgccgcc ttccgggccg agcgtgatgc cgctgggggt ggcgacgagg atgaagcgcg
     1801 aatcctggta gcaggcgtaa ttcaaggcat cgaggccgcg ggcgatgaac ggatcgtaga
     1861 gcgttccgac cgggaccagg cgggtcccga acaggtcgcc ggcgaggccc gccgcggcga
     1921 gcatcaggaa gagattgttc tcggcgattc cgagctcgat gtgctggccc gtgcttgtcg
     1981 ccgcccattt ttgcgcagag gcaatctttg ccttggcgaa gacgtcggcc gcctcgctgc
     2041 gccggaacag gccgcgctgg ttgacccagg cgccgaggtt ggtcgagacg gtgacgtcgg
     2101 gcgaggtggt gacgatgcgg tcggcgagcg gcccgccgga gcggctgagg tcgttgagga
     2161 tccgcccgaa cgccgcctgg gtcgattgct cgtcgccggc cggcgtcggg atgtgcggga
     2221 cctcgatcga gccgaacggc cgcgaccgct tttcccgcgc gaccgggcag cgctcgagca
     2281 gcgcctcgac cgccggccgg gcgttggcgc cggccgcttc gagcggctcc cactcgcgcc
     2341 cctcggcgac gcccatcgac tcgcgcagcg ccgccatctg ggtcgggttc atcagcccgg
     2401 cgtgattgtc cttgtggccg gcgaacggca gcccgtagcc cttgatcgtc caggcgatga
     2461 acagggtcgg cacgtcgtcc cgggcggcgt cgaacgcctc gaccagggtc cccatgcaat
     2521 ggccgccgag gtcggtgaac agggccgcga gcgcatcgtc gtcatgggat ttgaggaacg
     2581 ccgccgcgga cttgccgagg tcggcctcga tccgcgcccg ccacgccgcg ccgccctggt
     2641 agtggagcgc cgacagctcg gcgttcggaa gcccggcgag ccagcgtgcc atggccgggt
     2701 gcttgtcgag cgccgccgcc agcctcttgc cccagcgcag ctcgaccact cgccagccgc
     2761 agctcttgaa gatctcgtcg aagcgctcga acatccggtc agcgctcacc gcgtcgaggc
     2821 tctggcggtt gtagtcgacg atccaccaga cattgcggat gtcgtgcttg taggcctcga
     2881 tcagcgcctc gtagacattg ccttcgtcga gctcggcgtc gccgaccagg gcgacgaagc
     2941 ggccgcggtc ggcttcggcc agccagccgt gcgcgctcag ataatcctgc accaggctgg
     3001 cgaaggcggt gaccgcgacc ccaaggccga ccgacccggt cgagaagtcc accgggatcc
     3061 ggtccttggt ccggctcggg tagctctgca tgccgccgaa gccgcggaaa ttctccaggc
     3121 tttcgagcga ctggctgccg agcagataat ggatcgcgtg cagcaccggc ccggcgtgcg
     3181 gcttgaccgc gacccggtcg ttggggccga gcgcatggaa gtagagcgcc gccatgatcg
     3241 cggtcatcga cgtacagctc gcctggtggc cgccgacctt gagcccgtcg ggcgacgggc
     3301 ggacgtggtt ggcgtgatgg atggtccacg ccgacagcca gcgcagccgc tcctcgatga
     3361 tcctcagcgc gtcgaggtcg gtggtcgcgg gcttgagcat cgccgacgcc tagacgaggc
     3421 ggcctcgccc gctcaagcgg actcagtcgt ccgaagcgcc cgaaaaggcg tcgatcgcat
     3481 cgaagatgtc cgacacggcg gcccgctcgt cctcgccgag ctcgggcttc cagacgtcga
     3541 acagcaggat gatccgggtc tcgccgctcg gattcctcgc ttcatgctcc aggctgtcgt
     3601 cgaagaccag gagctcgccc tcgcgccatt cgcgcgtctc gttgccgacc cgcagccagc
     3661 cgccgggcgg gacgatcagc ggcagatggc agatcagccg gctgttgagc atgccgtggt
     3721 gcggcgggat gtgcgcgccc ggaagcaggc gggaaaacag cacggcgggg gtgcggcggc
     3781 cgatccggct gatcgggacc ttgtccagcg ccgccatggt cttcgggcag cggcgggcat
     3841 tctcctcgac gaccgcgccg tccttccaca ggtaaagcgc ggtccagctc tcgtcgccga
     3901 gaaggccgtg aaaatcgcgg tgcggccggt cctcctcggc cacgatatag gggcggaagt
     3961 cggcgccgtc ggcaagcagc gcctccagtt ccgcgcggat gaccggggcc tgcgcctcca
     4021 gctccgccgc ccagtcgaac tcgtcgcgtt cccagaactg ccgctgcgcc agatagggat
     4081 agtaaaggac cgacggctgc tgcgggtaga tgcttcgctt gcccttcagc atgtccatcg
     4141 cgtgggtcag ccgaagccgg tggatcgggc tggccggctc ggcgccctgg acgacgcgat
     4201 cgagcgcctg ctcgaactcc agcgcgcgct gctggacgaa ggcctgggcg cggcgcaggt
     4261 cggccatcgt ctcggaatcg acaagccgga ctccgcgcgc ggcaccgagc gccgcctggt
     4321 aaaaggacat ggcggcgcga tcgtcgccgt cccggacgta ggaatccccg cgaccgagca
     4381 aatcgcggat ctggcccgga tcggccgccg agctcatgcc gcttcggcct tgtcctgcgc
     4441 ggcgtcgaag cgggcctgcg ccgaatggat ctcctcgaca ttctcgagcg cccattgcgt
     4501 cagggccgtg atcggcctga gcagcgaatg gccgagcggg gtaagctcat aatcgacgcg
     4561 cggcggaatg ctcggcgtga cggtgcggtt gacgatcccg tcgcgctcga gattgcgcaa
     4621 ggtgagcgtc agcatgcgct gcgagacgct cgggatctcg cgccggagct cgttgaagcg
     4681 cctggggccg tcgccgaggg tcacgaccac gagcaccgtc catttgtcgc cgatccgcga
     4741 cagcagggtg ctgatcgtcc ggcagactgg gttgctgggg tccttcatgg tcgttctccg
     4801 gcgacacggc tgttcccgcg ccacaaattt gtgcgtcggc cacaaaaaag tgcgttcttg
     4861 cggccggttt ccacgtggtt acttagggtg cctcggtaac aaatccatac cagggaggca
     4921 atagtgacta tccttcacat cgacagcagc atcaacggcg acaacagcgt cagccgaatc
     4981 ctcacccggt cgatcgtcga ccagctgcgt gccgccaacc cgcgcgccgc gatcatctcg
     5041 cgcgatctcg ccagcgaacc actgccgcac ctgacgctcg acgcgttcgc tgattcgtcg
     5101 tcgctcgacg aattcctcgc cgccggctcg gtggtgatcg gg
//
LOCUS       DASTZO010000015         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1150225,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000015 DASTZO010000000
VERSION     DASTZO010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150225"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VFU20_00350"
     CDS             complement(<1..330)
                     /locus_tag="VFU20_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5284941.1"
                     /translation="MAEEFDFDVLVIGAGPGGYVAAIRAAQLGLRTACAESRATLGGT
                     CLNVGCIPSKALLHASELFEEAHDGTLAKWGVKAAKVELDLGQMHATRLEAVKGLTQG
                     IEFLFKKN"
     gene            complement(353..1702)
                     /gene="odhB"
                     /locus_tag="VFU20_00355"
     CDS             complement(353..1702)
                     /gene="odhB"
                     /locus_tag="VFU20_00355"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HEU5284942.1"
                     /translation="MATDVQVPALGESITEGTLAQWLKQSGDAVAADEPIASLETDKV
                     TVEVPSPVAGVFGEALVKEGDTVAVGAVIARIDEGAQATAAPAAATASSPAAEAADAT
                     TNPAGAGETPTLRGDDHAPEPERRASAADTPAADSAEAHGGDTIITLSPAVRRAVLEH
                     HVDPSKIKGTGKDGRLTKDDVIAAAEAQKAGVQPQARASAPPAAAKPAAPAAAATGER
                     KEERVKMSRLRQTIAARLKEAQNTAALLSTFNDVDMSAVIEARARYKDLFEKKHGIRL
                     GFMGFFVKAVALATRDLPAVNARIDGDEIVYHDYLDVSIAVSAPKGLVVPVVRNAQAM
                     SFAEIEKTIADYGKRAKEGTLGIEEMQGGTFTISNGGVFGSLLSTPIINPPQSAVLGM
                     HRIEERPVVRDGQVVVRPMMYLALSYDHRLVDGREAVTFLVRVKEAIEDPTRLLIDL"
     gene            complement(1712..>2051)
                     /gene="sucA"
                     /locus_tag="VFU20_00360"
     CDS             complement(1712..>2051)
                     /gene="sucA"
                     /locus_tag="VFU20_00360"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683098.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HEU5284943.1"
                     /translation="IEQLYPFPSEPLVKRLKAMPGLEQLVWAQEEPKNNGAWLFVEPL
                     LECCLKEAGVAGMRPQYAGRDSAASPATGLAKRHAAEQAALIAGALGHPSTKPEAGSS
                     ERADSPAAAQ"
BASE COUNT          258 a          738 c          705 g          350 t
ORIGIN      
        1 gttcttcttg aacaggaact caatgccctg ggtgagcccc ttgaccgctt ccaatcgcgt
       61 cgcgtgcatc tggccgaggt cgagctcgac cttcgcggcc ttgacgcccc atttggcgag
      121 cgttccgtca tgcgcttcct cgaacagctc ggacgcgtgg aggagcgcct tcgacgggat
      181 gcagccgacg ttgaggcagg tgccgcccag ggtcgcgcgg ctttcggcgc acgcggtccg
      241 gaggccgagc tgagcggcgc ggatcgccgc gacatagccg ccgggcccgg cgccgatcac
      301 cagcacgtcg aaatcgaact cttccgccat tctgtttctt tcgtgagggt ccttaaaggt
      361 cgatcagcag ccgcgtcggg tcctcgatcg cttccttgac ccggaccagg aaggtgaccg
      421 cctcgcgccc gtcgaccagc cggtggtcgt agctcagcgc caggtacatc atcggccgca
      481 ccacgacctg gccgtcgcgg accaccggcc gctcctcgat gcggtgcatg cccagcaccg
      541 ccgattgcgg cgggttgatg atcggcgtcg acagcaggct gccgaacacg ccgccgttgg
      601 agatggtgaa ggtgccgccc tgcatctcct cgatgcccag cgtcccttcc ttggcccgct
      661 tgccgtagtc ggcgatcgtc ttctcgatct cggcgaagct catcgcctga gcgttgcgga
      721 ccaccggcac caccagcccc ttgggcgccg agaccgcgat cgagacgtcg agatagtcgt
      781 ggtagacgat ctcgtcgccg tcgatgcgcg cgttgaccgc cggcaggtcg cgggtggcga
      841 gcgcgacggc tttgacgaag aagcccatga agccgagccg gatgccgtgc ttcttctcga
      901 acaggtcctt gtagcgggcg cgcgcctcga tcaccgccga catgtcgacg tcgttgaagg
      961 tggacagcag cgccgccgtg ttctgcgcct ccttcagccg cgcggcgatg gtctggcgaa
     1021 ggcgcgacat cttgacgcgc tcttccttgc gctcgccggt ggcagcagcc gcgggtgccg
     1081 caggcttcgc cgccgccgga ggcgcggagg cccgggcctg gggctggacg cccgccttct
     1141 gcgcctcggc ggccgcgatc acgtcgtcct tggtcagccg cccgtccttg ccggtgccct
     1201 tgatcttcga cgggtcgacg tgatgctcga ggaccgcgcg ccgaaccgcc ggcgacaggg
     1261 tgatgatcgt gtcgccgccg tgcgcctccg ccgagtccgc cgccggagtg tcggcggcgc
     1321 tcgcccgccg ctcgggctcg ggcgcatgat cgtcgccgcg cagggtcggc gtctcgcccg
     1381 cgccggcggg gttggtggtc gcgtcggccg cttcggcagc cggcgaggag gcggtcgctg
     1441 cggcgggcgc ggccgtggct tgcgcgcctt cgtcgatccg ggcgatcacc gccccgaccg
     1501 cgaccgtgtc gccttccttg accagcgcct cgccgaacac tccggcgacc ggcgagggca
     1561 cctcgaccgt gaccttgtcg gtctcgaggc tggcgatcgg ctcgtcggcg gcgaccgcgt
     1621 cgcccgactg cttcagccac tgggcgagcg tcccctcggt gatcgactcc ccgagcgccg
     1681 ggacctgaac gtcagtcgcc atgttgatcc ttcactgggc cgcggccgga ctgtccgcgc
     1741 gctcactgct acctgcctcg ggctttgtcg aggggtggcc gagagctccg gcgatcaggg
     1801 ccgcctgctc ggccgcgtgg cgcttggcga ggccggtcgc cggcgacgcg gccgaatcgc
     1861 gccccgcata ttgcggccgc atgcccgcga cgcccgcctc cttgaggcag cactcgagca
     1921 gcggctcgac gaacagccag gcgccgttgt tcttcggctc ctcctgcgcc cagaccagct
     1981 gctcgagccc gggcatcgcc ttcagccgct tgaccaaggg ctcggagggg aacgggtaga
     2041 gctgctcgat c
//
LOCUS       DASTZO010000016         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_288511,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000016 DASTZO010000000
VERSION     DASTZO010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_288511"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /gene="pip"
                     /locus_tag="VFU20_00365"
     CDS             complement(<1..555)
                     /gene="pip"
                     /locus_tag="VFU20_00365"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEU5284944.1"
                     /translation="MDIQVTERRTLYPLAEPCKSGHLDVGDGHSLYWELCGNPDGKPA
                     VFLHGGPGSGISVRHRGQFDPKRYNVLLFDQRGAGKSTPHAGLEANTTWHLVDDLERL
                     RTMVGVDRWMVFGGSWGSTLALAYAQTHPQRTTELVLRGIFMLRRWELEWFYQEGANR
                     LFPDAWEHYIAAIPPVERHDLISAF"
     gene            complement(556..1926)
                     /locus_tag="VFU20_00370"
     CDS             complement(556..1926)
                     /locus_tag="VFU20_00370"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HEU5284945.1"
                     /translation="MTTRTDTSASPAIESRDDLLSVFSGGEKARDDWRIGTEHEKFVY
                     RCDDHRAPSWEEPGGIRDLLLGLTEFGWRPVEEEGKVIALTGPDGTISLEPAGQFELS
                     GAALTDLHMTCAEAARHLQQCKTVGDRLGVGFLGLGMWPDKRRDELPMMPKGRYAIMA
                     RHMPRVGKLGLDMMLRTCTIQVNLDYSSEADMVKKFRVGLALQPVATALFANSPLTEG
                     KPNGYKSYRSHIWEDTDPHRTGMLPFVFDEGFGYERYCDYALDVPMYFVFRDGQYIDV
                     AGESFRTFLDGKLPQLPGEKPLLSDFTDHLSTAFPEVRLKSFLEMRGADGGRWGRICA
                     LPALWVGLLYADEALDAAWDRVRHWTIEEREQLRHDVPRLALEAPVPGGGTVHDLARE
                     VLDLAALGLNRRGVLNAAGDNEGGFLDPLRDVVASGKTPADRLLEKYHGEWGGDVRRI
                     YEEMSF"
     gene            complement(1954..>2262)
                     /locus_tag="VFU20_00375"
     CDS             complement(1954..>2262)
                     /locus_tag="VFU20_00375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HEU5284946.1"
                     /translation="RTRLPDISEPLPLPHFLQFREASRALYFADEAGGEAAAGAFTSG
                     PAAILTGPEGGFTDEERSAIRAAPNAVPVSLGPRILRAETAALAALAAFMAVAGDWRG
                     "
BASE COUNT          332 a          768 c          804 g          358 t
ORIGIN      
        1 gaaggccgag atcaggtcgt gccgctccac cggcgggatc gcggcgatgt agtgctccca
       61 cgcgtccggg aacaggcggt tggcgccttc ctggtagaac cactccagct cccagcgacg
      121 cagcatgaag atgccgcgca ggaccagctc ggtggtgcgc tgcggatgcg tctgcgcgta
      181 ggccagcgcc agggtcgagc cccacgagcc gccgaacacc atccagcggt cgactccgac
      241 catcgtccga agccgctcga ggtcgtcgac cagatgccaa gtcgtgttcg cctcgagccc
      301 cgcgtgcggg gtcgattttc cggcgccgcg ctggtcgaac agcagcacat tgtagcgctt
      361 cgggtcgaac tgcccgcggt gcctgacgct gatcccggat cccgggccgc cgtgaaggaa
      421 cacggccggc ttgccgtccg ggttgccgca cagctcccag tagaggctgt ggccgtcgcc
      481 gacgtcgaga tggcccgact tgcagggctc cgccagcggg tagagcgtcc ggcgctcggt
      541 cacctgaatg tccatctaaa agctcatttc ctcgtagatt cggcggacgt cgccgcccca
      601 ctcgccgtga tatttttcca gcaaccggtc ggcgggcgtc ttgccgcttg cgaccacgtc
      661 gcgcaacggg tcgaggaatc cgccctcatt gtcgccggcg gcgttgagca cccctcgccg
      721 gttgaggccg agcgcggcca ggtcgagcac ctcgcgggcc aggtcgtgga ccgttccgcc
      781 gcccggcacc ggcgcctcga gcgcgagcct ggggacgtcg tggcgcagct gctcgcgctc
      841 ctcgatcgtc cagtgccgga cgcggtccca ggcggcgtcg agcgcctcgt cggcgtagag
      901 cagcccgacc cacagcgccg gaagcgcgca gatgcggccc cagcgcccac cgtcggcgcc
      961 gcgcatctcg aggaagcttt tcagccgaac ctcggggaat gcggtcgaca gatggtcggt
     1021 gaagtcgctg agcagcggct tctcgccggg cagctgcggc agcttgccgt cgaggaaggt
     1081 gcggaagctc tcgccggcga cgtcgatgta ctggccgtcg cggaacacga agtacatcgg
     1141 cacgtcgagc gcgtagtcgc agtagcgctc gtagccgaag ccctcgtcga acacgaacgg
     1201 cagcatgccg gtgcggtgcg ggtcggtgtc ctcccagatg tggctccggt agctcttgta
     1261 gccgttgggc ttgccctcgg tcagcggcga gttggcgaac agcgccgtcg ccaccggctg
     1321 cagcgctagg ccgacccgga acttcttcac catgtcggcc tcggacgagt aatcgaggtt
     1381 gacctggatg gtgcaggtgc ggagcatcat gtcgagtccg agcttgccga cccgcggcat
     1441 gtggcgggcc atgatcgcgt agcggccctt gggcatcatc ggcagctcgt cgcggcgctt
     1501 gtcgggccac atgccgaggc cgaggaagcc gactcccagg cggtcgccga cggtcttgca
     1561 ctgctgcagg tgccgcgccg cctcggcgca ggtcatgtgt aggtcggtca gggccgcgcc
     1621 cgacagctcg aactggccgg cgggctcgag gctgatggtg ccgtcggggc cggtcagcgc
     1681 gatcaccttc ccctcctcct cgaccggccg ccagccgaat tcggtgaggc cgagcaagag
     1741 gtcgcggatc ccgcccggct cctcccacga cggggcgcgg tgatcgtcgc agcggtagac
     1801 gaacttctcg tgctcggtgc cgatgcgcca gtcgtcgcgc gctttctcgc cgccggagaa
     1861 taccgacagc aagtcatcgc ggctttcgat ggccgggctg gcgctggtgt cggtgcgggt
     1921 ggtcatgtgg ggcggcgccc tagccgtgcg gcgctatccg cgccagtccc cggcgaccgc
     1981 catgaaggcg gcgagcgcgg ccagagccgc ggtttcggcg cgcaggatgc gcggtccgag
     2041 cgagaccggg accgcgttgg gcgcggcgcg gatcgccgag cgttcctcgt cggtgaagcc
     2101 gccctcgggc ccggtcagga tcgcggccgg cccagaagta aaggcgcccg ccgccgcttc
     2161 gccgcccgct tcgtcagcaa aatagagcgc tctcgaagct tcgcggaatt gaaggaaatg
     2221 gggaagcggc agcggctcgg aaatgtcggg tagccgggtc cg
//
LOCUS       DASTZO010000017         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1280528,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000017 DASTZO010000000
VERSION     DASTZO010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280528"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFU20_00380"
     CDS             <1..478
                     /locus_tag="VFU20_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase family protein"
                     /protein_id="HEU5284947.1"
                     /translation="SVTTEELLLDRASLLGLSVPEMTVLLGGLRVLGANYKDAPEGVF
                     TTRKGQLTNDFFVNLLDNDTFWQVVDTSGDELFIGYDRGGKQEKWRATRTDLIFGSNS
                     QLRAVAEVYAERGNEEKFVRDFVHAWTKVMNADRFDLPQKPDSVEPIGVAEPAMAK"
     gene            complement(481..1476)
                     /locus_tag="VFU20_00385"
     CDS             complement(481..1476)
                     /locus_tag="VFU20_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5284948.1"
                     /translation="MTTYLPTIRQLQYLIALKDYGHFGKAAESCFVTQSTLSASLREL
                     EALVGVTLVERTRRVVRFTPLGAKIADKARRVLRETEELADMARAEGQPLHGELRMGV
                     IPTIAPFLLPAMLPRLREQWPSLKIFLREEASQAACDALHRGQLDCVLLAMPYACGDV
                     DTAMLFDDRLFVAFPGGEAPKGTPGGASVEAAAIDENRLLLLEDGHCLKDHALAACNR
                     PELRAEAAMMGTSLHTLVQMVDNGLGLTFVPSMAIEAGILDGTDIEARPLRSDHGYRR
                     IALVWRRSSPREEEFRLLAETLRQISRKVIPSLAAAIPGDAAARGEGRARRGIGS"
     gene            complement(1512..2303)
                     /locus_tag="VFU20_00390"
     CDS             complement(1512..2303)
                     /locus_tag="VFU20_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007707241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5284949.1"
                     /translation="MFPSVLADPAVLLPFIAIGFAAQMVDGALGMAFGQISSTLLISM
                     GVPPAAASAGVHTAETFTTGVSGISHVAHGNVDWRLFFRIVIPGVIGGVAGAYLLSNV
                     SAEAAKPVVLTYLTLLGLYLFYRGVMHRHTERRPRIVAPLGLAGGFLDAAGGGGWGAI
                     VTSNLLVQGSNPRRTIGTVNTAEFFLTVTISATFIAALGWEAFTIATLGLLIGGVAAA
                     PFGAWIAKRVNPDRLLTLVGALVTLTSGYALYRVLTRAEALALIA"
     gene            2449..>2919
                     /gene="epsC"
                     /locus_tag="VFU20_00395"
     CDS             2449..>2919
                     /gene="epsC"
                     /locus_tag="VFU20_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase EpsC"
                     /protein_id="HEU5284950.1"
                     /translation="MISGLVDYLDSVKARDPAAHSRWEVLLYPGVLALGMHRIGHWLY
                     GGRLYFLARVVNHLARFLTAIDIHPGARIGRNFFIDHGFTVIGETAEIGDDVTIYQNV
                     TLGGTNPSTGIGGKRHPTLRDNVIIGSGAQIIGPIVVGERARVGANAVVTEDVPE"
BASE COUNT          471 a         1005 c         1016 g          427 t
ORIGIN      
        1 ttcggtgacg accgaggagc tgctcctcga ccgagcgtcg ctgctcggcc tgtcggtgcc
       61 ggaaatgacg gtgctgctgg gtgggctgcg cgtgctcggc gccaactaca aggatgctcc
      121 ggagggcgtc ttcacgaccc gcaagggcca gctgaccaac gacttcttcg tcaatctgct
      181 cgacaacgac accttctggc aggtggtcga tacaagcggc gacgagctgt tcatcggcta
      241 cgatcgcggc ggcaagcagg agaagtggcg cgcgacccgc accgacctga tcttcggctc
      301 caacagccag ctgcgcgcgg tggccgaggt ctatgccgag cgcggcaacg aggagaagtt
      361 cgtccgcgac ttcgtccacg cgtggaccaa ggtgatgaac gccgaccgct tcgacctccc
      421 gcagaagccc gacagcgtcg agccgatcgg agtcgccgag ccggccatgg cgaagtagcg
      481 ctagcttcca atgccgcggc gcgcgcgccc ctccccgcgc gccgcggcat ccccgggaat
      541 ggccgcggcc agcgagggaa tgactttccg gctgatctgc cggagcgtct cggcgagcag
      601 ccggaactct tcctcgcgcg ggctcgagcg gcgccagacg agcgcgatcc gccggtagcc
      661 gtggtccgag cgcagcggcc gcgcctcgat gtcggtgccg tcgaggatcc ccgcctcgat
      721 cgccatcgac gggacgaacg tcaggccgag gccgttgtcg accatctgca ccagcgtgtg
      781 cagcgaggtg cccatcatcg ccgcttcggc gcggagctcg ggccggttgc acgcggcgag
      841 cgcatggtcc ttgaggcaat ggccgtcctc gagcagcagc agccggttct cgtcgatcgc
      901 cgccgcctcg accgaggctc cgcccggggt cccctttggc gcctcgccgc ccgggaaggc
      961 gacgaacagc cggtcgtcga acagcatggc ggtgtcgacg tcgccgcacg cgtagggcat
     1021 cgccaggagc acgcagtcga gctgcccgcg gtgaagcgcg tcgcaggccg cctggctcgc
     1081 ttcctcgcgg aggaagatct tgaggctcgg ccattgctcg cgaagccgcg gcagcatcgc
     1141 cggcagaagg aaaggcgcga tggtcgggat cacccccatg cgcaactcgc cgtgcagcgg
     1201 ctggccttcg gcgcgcgcca tgtcggccag ctcctcggtc tcgcgcagca cccggcgcgc
     1261 cttgtcggcg atcttcgctc cgagcggcgt gaagcgcacc acccgccggg tgcgctcgac
     1321 cagggtgacc ccgaccagcg cctcgagctc gcgcaggctc gccgacaggg tcgactgggt
     1381 gacgaagcag ctttcggccg ccttgccgaa atggccgtag tccttgagcg cgatcagata
     1441 ttgaagctgg cggatggtgg ggaggtaggt cgtcatgggc ggtcccgatc agtcccgccc
     1501 ggataagcgg ttcaggcgat taacgccagc gcctcggccc tggtgagcac tcggtagagc
     1561 gcgtagccgc tggtcagcgt caccagcgct ccgaccaggg tcagcagccg gtcggggttg
     1621 acccgcttgg cgatccacgc gccgaacggc gccgccgcca ccccgccgat cagcagcccc
     1681 agagtggcga tggtgaaggc ttcccagccg agcgcggcga tgaaggtcgc cgaaatggtg
     1741 acggtgagga agaactcggc ggtgttgacg gtaccgatgg tgcgcctcgg gttgctgccc
     1801 tggaccagca ggttggaggt gacgatcgct ccccagccgc cgccgccggc cgcgtcgaga
     1861 aagccgccgg caaggccgag cggggcgacg atcctgggcc gccgctcggt gtggcggtgc
     1921 atcaccccgc ggtagaacag gtacaggccg agcagggtca gatacgtcag gaccaccggc
     1981 ttggccgcct cggcgctgac gttggacagc agataggcgc ccgcgacccc gccgatcacc
     2041 ccggggatga cgatccggaa gaacagccgc cagtcgacat tgccgtgggc gacgtggctg
     2101 atccccgaga cgccggtggt gaaggtctcg gcggtgtgca ccccggccga ggccgccgcc
     2161 ggcggaacgc ccatgctgat cagcagggtg ctcgagatct gcccgaacgc catcccgagc
     2221 gcgccgtcca ccatctgggc ggcgaagccg atggcgatga acggcagcag gaccgccgga
     2281 tcggccagga cggacgggaa aatctcttgt ctccacccct gattgcgccg cgcgatgtgc
     2341 cgaaccgccg accgcgccgc aagcgaaacc ggatgcgccg gcctcggccc ggctggcatt
     2401 tcgggcggct cgcacctaga ttgcggggat gaggagcaag gggatcgcgt gatttcaggg
     2461 cttgtcgact atctggattc ggtcaaggcg cgcgacccgg cggcgcattc gcgctgggag
     2521 gtgctgctct atccgggcgt gctggcgctc gggatgcacc gcatcggcca ctggctgtac
     2581 ggcgggcggc tctatttcct cgcccgggtc gtcaaccatc tggcgcgctt cctgaccgcg
     2641 atcgacatcc atcccggcgc caggatcggc cgcaacttct tcatcgacca cggcttcacg
     2701 gtgatcggcg agaccgccga gatcggcgac gacgtcacca tctaccagaa cgtcaccttg
     2761 ggcggcacca acccgtcgac cggcatcggc ggcaagcgcc acccgacgct gcgcgacaac
     2821 gtcatcatcg gctcgggcgc acagatcatc ggcccgatcg tggtcggtga gcgcgcccgc
     2881 gtcggcgcga acgcggtcgt caccgaggac gtccccgaa
//
LOCUS       DASTZO010000018         5144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1150286,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000018 DASTZO010000000
VERSION     DASTZO010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5144
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1150286"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..769)
                     /locus_tag="VFU20_00400"
     CDS             complement(<1..769)
                     /locus_tag="VFU20_00400"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HEU5284951.1"
                     /translation="MPRLATLLIATAALAACDQPRTDAPPANAVAPAGPAQLSLTRLD
                     CGSATIKDFDAFFSDRPGLYAKGPREITDSCYLIRHRSPMGDRAMVWDTGFAAGLKGR
                     SQDMGELVARLGSTIAEQLARLGLKPGDVDIVGISHMHPDHTGQAAQFPNAALLIGKR
                     DFEQTAGKDDPFGPWRGAGKAVTLVGADHDVFGDGSVTALHLPGHTPDHLALLVKLAS
                     GPVLLSGDLYHSTEARAKRGVPPFNTDRAQTLASMDRF"
     gene            complement(781..1059)
                     /locus_tag="VFU20_00405"
     CDS             complement(781..1059)
                     /locus_tag="VFU20_00405"
                     /inference="COORDINATES: protein motif:HMM:NF042503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6694 family lipoprotein"
                     /protein_id="HEU5284952.1"
                     /translation="MKAVAAGLLLFLAACGNERPVAIDGSSAEAFERSAAEARRQLPD
                     AERLLFDRAIRTVGGRRHSERDPGALARVTFDGMTAREVVADQRAREK"
     gene            complement(1056..1691)
                     /locus_tag="VFU20_00410"
     CDS             complement(1056..1691)
                     /locus_tag="VFU20_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HEU5284953.1"
                     /translation="MPWDRNQMAARAAKELRDGYYVNLGIGIPTLVANHIPAGLTVTL
                     QSENGMLGIGPFPYEDEVDPDLINAGKQTISELPSSSYFSSSDSFAMIRGGHIDLTVL
                     GAMEVSEKGDIANWMIPGKMIKGMGGAMDLVAGVKKIIVVMEHVSKNGDPKFIPECTL
                     PLTGQNVVDMIITDLAVFQRPDHDSPFRLIERAPDVTAEEIGSKTSARYEE"
     gene            complement(1691..2398)
                     /locus_tag="VFU20_00415"
     CDS             complement(1691..2398)
                     /locus_tag="VFU20_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018251084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HEU5284954.1"
                     /translation="MKKIYPDAKAALDGLLFDGMTIAAGGFGLCGIPERLIDAIVASG
                     VRDLTIASNNAGIDNEGLGKLLRTRQVKTMISSYVGENKEFERQYLAGELEVEFCPQG
                     TLAERMRAGGAGIPGFYTKTGVGTVVAEGKELKEFDGETYVLERGIRADLSIIKGWKA
                     DEDGNLLFRKTARNFNQPAATCGRVCVAEVEKIVPVGSLDPDCIHLPSIYVKRLICGA
                     PYDKKIEFRTVREREAA"
     gene            complement(2409..2708)
                     /locus_tag="VFU20_00420"
     CDS             complement(2409..2708)
                     /locus_tag="VFU20_00420"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HEU5284955.1"
                     /translation="MTKQELVAKMQAGQAWVPGKRVKLDFGGEGTVMLDGAASQVSED
                     DGEADTVIKVGWDDWQQMAAGQLDGMTAFMTGKLKVEGDMSNAMQLQGVLAKLRG"
     gene            2784..4211
                     /locus_tag="VFU20_00425"
     CDS             2784..4211
                     /locus_tag="VFU20_00425"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HEU5284956.1"
                     /translation="MGYEADLKLFIDGSWRAGEDRDHFTVVNPVTGSGIAELPLASDS
                     DLEEALAAAERTWPEWRSTDVEKRSAILHKTADLLRERAEHIGRILTQEQGKIFPEAR
                     AEVLGSAQLFDWYAEEIKRDYGRTLVRPAGQLSRVIRQPVGPCATFTPWNFPIYLLAK
                     KVAAALASGCTVISRPPQETPGCTGELFRALAEAGIPAGVAQLVHGNGSHISEKLLGS
                     KVIRKVSFTGSVPVGKTLMKLAADSMTRITLELGGHAPVLIFDDCNLEKTLDMVVPQK
                     FRNAGQVCVSPTRFYVQRGIYDAFLKGFAERTAKVKVGDGLAEGTKMGPLANARRPET
                     VGALIEDAKQRGARVLAGGGPGDGGFFFQPTLLADVPNEAEIMNEEPFGPVAVTRAFD
                     TFDEAIHEANRLPYGLAAFAFTENGRRANLLGDSIEAGMVGINTFAISVADAPFGGVK
                     DSGFGNEGGKEGLETYQVVKAIHQA"
     gene            complement(4440..>5144)
                     /locus_tag="VFU20_00430"
     CDS             complement(4440..>5144)
                     /locus_tag="VFU20_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5284957.1"
                     /translation="ATVIFDKPQPVRVLALIDAKGGASSSADSDAELQALMVQAAGRT
                     LDGSGQAPVRDKVIGDLRDGLKLASFTWHKPVADKEVAGYREALLNIIETPNAALFHV
                     NGRPFDHDRTDMVLPLGKAEEWHAVSLLGAHPLHMHVNPFQIVRIQNSKGDDVTDPKG
                     PAFDPDYAGLKGEWKDTIFLKQDLRVAFRTRYERFTGDFVTHCHIMVHGDEGMMLKLS
                     IADDGEAKKVAQHEAH"
BASE COUNT          777 a         1805 c         1692 g          870 t
ORIGIN      
        1 cgaaccggtc catcgaggcg agcgtctgcg cccggtcggt gttgaacggc ggcacgccgc
       61 gcttcgccct cgcctcggtc gaatggtaga ggtcgccgct cagcagcacg ggcccgctcg
      121 ccagcttgac cagcagcgcc agatggtcgg gcgtgtgccc cggcaaatgc agcgccgtca
      181 cgctgccgtc gccgaacacg tcgtggtcgg cgccgaccag ggtcaccgct ttgcctgccc
      241 cgcgccacgg cccgaacggg tcgtccttgc ccgcggtctg ttcgaaatcg cgcttgccga
      301 tcagcagcgc cgcgttcggg aattgcgccg cctggccggt gtggtcgggg tgcatgtggc
      361 tgattccgac gatgtcgacg tccccgggct tcagcccgag ccgcgcgagc tgctcggcga
      421 tcgtcgagcc gagccgcgcg acgagctcgc ccatgtcctg cgagcggccc ttcaggccgg
      481 ccgcaaagcc ggtgtcccag accatcgccc ggtcgcccat cgggctgcgg tgccggatca
      541 gatagcagct gtcggtgatc tcgcgcgggc ccttcgcgta gaggccgggc cggtcggaga
      601 agaaggcgtc gaaatccttg atcgtcgcgc tgccgcagtc gagccgggtc aggctgagct
      661 gagccggccc ggccggcgcc acggcgttgg cgggcggcgc atcggtccgc ggctggtcgc
      721 aggcggcgag cgccgcggtc gcaatcagga gcgtggcaag cctgggcatc ggaatccctc
      781 ctatttctcg cgcgcgcgct ggtcggcgac gacctcacgc gccgtcatgc cgtcgaaggt
      841 cacccgcgcc agcgcgcccg gatcgcgctc cgagtgccgg cgcccgccga cggtccggat
      901 cgcgcggtcg aacagcagcc gctcggcgtc gggaagctgg cgccgagcct cggcggcgct
      961 gcgctcgaac gcctcggccg aggagccgtc gatcgccacc ggccgctcgt tcccacaggc
     1021 cgcgaggaac agcagcaggc ccgcggcgac cgctttcatt cctcgtaacg cgcgctggtc
     1081 ttggagccga tttcctcagc cgtgacgtcg ggggcccgct cgatcagccg gaacgggctg
     1141 tcgtggtcgg gccgctggaa caccgcgagg tcggtgatga tcatgtcgac gacgttctgg
     1201 ccggtcagcg gcagggtgca ctcggggatg aacttggggt cgccgttctt cgacacatgc
     1261 tccatcacga cgatgatctt cttgaccccg gcgaccaggt ccatcgcccc gcccatgccc
     1321 ttgatcatct tgccggggat catccagttg gcgatgtcgc ctttttccga cacctccatc
     1381 gcgccgagca ccgtcaggtc gatatggccg ccgcggatca tcgcgaagct gtccgacgag
     1441 ctgaaatagg aggacgacgg aagctcgctg atggtctgct tgccggcgtt gatcaggtcc
     1501 gggtcgacct cgtcctcgta cgggaacggc ccgatcccga gcatcccgtt ctcgctctgc
     1561 agggtcaccg tcagcccggc cgggatgtgg ttggcgacca gcgtcgggat gccgattccg
     1621 aggttgacgt aatagccgtc gcgaagctcc ttcgccgccc gcgcggccat ctggttgcgg
     1681 tcccagggca ttatgccgcc tcccgctcgc ggacggtccg gaactcgatc ttcttgtcgt
     1741 agggcgcgcc gcagatcagc cgcttcacgt agatcgacgg cagatggatg cagtcggggt
     1801 cgaggctgcc gacgggcacg atcttctcga cctcggcgac gcagactcgc ccacaggtcg
     1861 cggccggctg gttgaagttg cgggcggtct tgcggaacag caaattgccg tcttcgtcgg
     1921 ccttccagcc cttgatgatg gaaaggtcgg cgcggatgcc gcgctcgagc acgtaggtct
     1981 cgccgtcgaa ctccttcaat tccttgccct cggcgacgac cgtgccgacg cccgttttgg
     2041 tatagaagcc ggggattccg gcgccgccgg cgcgcatccg ctcggcgagc gtcccctgcg
     2101 ggcagaattc gacctcgagc tcgccggcga gatattgccg ctcgaactcc ttgttctcgc
     2161 cgacgtagga cgagatcatt gtcttcacct ggcgggtgcg gagcagcttg ccgagcccct
     2221 cattgtcgat gccggcgttg ttcgaggcga tggtcaaatc cctgaccccg ctggcgacga
     2281 tcgcgtcgat cagccgctcg gggatcccgc acaggccgaa cccgccggcc gcgatggtca
     2341 tgccgtcgaa cagcagcccg tcgagcgcgg ccttcgcatc ggggtagatt ttcttcatcc
     2401 gccgcccttc agccccgcag cttggccagc acgccctgca gctgcatcgc gttggacatg
     2461 tcgccctcga ctttcagctt gccggtcatg aacgcggtca ttccgtccaa ttggccggcg
     2521 gccatctgct gccagtcgtc ccagccgacc ttgatcaccg tgtcggcctc gccgtcgtcc
     2581 tcgctgactt ggctcgcggc gccgtcgagc atcaccgtcc cctcgccgcc gaagtcgagc
     2641 ttgacgcgct tgccgggcac ccacgcctgc cccgcctgca tcttcgccac cagttcctgc
     2701 ttggtcatgc cctgcgtctc cttttcgggg ccccgcctag cgaagcgttg cagccgcttc
     2761 aaccgaggcc tatctgcgcc gccatgggat atgaagcaga cttgaagctg ttcatcgacg
     2821 gcagctggcg cgccggcgag gaccgcgacc atttcaccgt cgtcaacccg gtcaccggga
     2881 gcggcatcgc cgagcttccg ctggccagcg acagcgacct cgaggaggcg ctggccgcgg
     2941 ccgagcggac ctggcccgaa tggcggtcga ccgacgtcga gaaacgcagc gcgatcctcc
     3001 acaagaccgc cgacctgctg cgcgagcgcg ccgagcatat cggccgcatc ctgacccagg
     3061 agcagggcaa gatctttccc gaggcgcgcg ccgaagtcct cggcagcgcg caactgttcg
     3121 actggtacgc cgaggagatc aagcgcgact acggccgcac cctggtgcga ccggccgggc
     3181 agctgtcgcg ggtgatccgc cagccggtcg gcccgtgcgc gaccttcacc ccgtggaact
     3241 tcccgatcta tctgctcgcc aagaaagtcg ccgcggcgct cgccagcggc tgcacggtga
     3301 tctcgcgccc gccgcaggaa accccgggct gcacaggcga attgttccgc gccctggccg
     3361 aggccggaat ccccgcggga gtcgcgcagc tggtccacgg caacggcagc cacatcagcg
     3421 agaagctgct cggctcgaag gtcatccgca aggtcagctt caccggctcg gtgccggtcg
     3481 gcaagacgct gatgaagctc gccgccgact ccatgacccg gatcacgctc gagctcggcg
     3541 gccacgcgcc ggtgctaatc ttcgacgatt gcaaccttga gaagacgctc gacatggtcg
     3601 tgccgcagaa gttccgcaac gccggccagg tgtgcgtctc gccgacccgc ttctacgtcc
     3661 agcgcgggat ctacgatgcg ttcctcaagg gcttcgccga gcgcaccgcc aaggtgaagg
     3721 tcggcgacgg gctcgccgag ggcaccaaga tgggcccgct tgccaacgcc aggcggcccg
     3781 agacggtcgg cgctttgatc gaggacgcca agcagagggg cgcgcgcgtg ctcgccggcg
     3841 gcggccccgg agacggcggt ttcttcttcc agccgaccct gctcgccgac gtccccaacg
     3901 aggccgagat catgaacgaa gagccgttcg gcccggtcgc ggtgacgcgc gcgttcgaca
     3961 cgttcgacga ggcgatccac gaggccaacc gcctgcccta cggcctcgcc gcgttcgcct
     4021 tcaccgagaa cggccgccgc gccaatctgc tcggcgactc gatcgaggcc ggcatggtcg
     4081 gaatcaacac cttcgccata tcggtcgccg acgcgccgtt cggcggggtc aaggacagcg
     4141 gcttcggaaa cgaaggcggc aaggaaggcc tcgagaccta ccaagtggtc aaggcgatcc
     4201 accaggcctg acttgagctg ccgggcgcag gtgagcgaat gcgcagcatt cgggagccag
     4261 cgcgtagagc cggcgaaagc cggacggcct gcgcacgtca gcgcgacagg accgacggcg
     4321 cggattcact ccgcgccggc ttttgccttg tgtcgaacag acgacgtctt cccgaccttt
     4381 gaccgcattc cccactttcg caacgtgatc gacgtgcgcc ttgggtcctc ccgaaggcgc
     4441 tagtgcgcct cgtgctgcgc cactttcttc gcttccccgt catcggcgat gctcagcttg
     4501 agcatcattc cctcgtcgcc gtggaccatg atgtggcagt gggtgacgaa gtccccggtg
     4561 aagcgctcgt agcgggtgcg gaaggcgacc cgcaggtcct gcttcaagaa gatcgtgtcc
     4621 ttccactcgc ccttcagccc ggcatagtcg ggatcgaagg ccgggccctt ggggtcggtg
     4681 acgtcgtcgc cctttgagtt ttggatcctg acgatctgga acgggttcac gtgcatgtgc
     4741 agcgggtgcg cgcccagcag cgaaaccgca tgccattcct ccgccttgcc gagcggcagg
     4801 accatgtccg tccggtcgtg gtcgaacggg cgcccgttga cgtggaacaa ggccgcattg
     4861 ggggtctcga tgatgttgag cagcgcctcg cggtagccgg cgacctcctt gtccgcgacc
     4921 ggcttgtgcc aggtgaatga ggcgagcttc agcccgtcgc gcaggtcgcc gatgaccttg
     4981 tcgcggaccg gcgcctggcc gctcccgtca agcgtccgac cggccgcctg caccatcagc
     5041 gcctggagct cggcatccga atcggccgag ctgctcgcgc cgcccttggc atcgatcaac
     5101 gccagcaccc gcaccggctg gggcttgtcg aagatcaccg tggc
//
LOCUS       DASTZO010000019         2981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_419282,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000019 DASTZO010000000
VERSION     DASTZO010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2981
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419282"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VFU20_00435"
     CDS             complement(<1..930)
                     /locus_tag="VFU20_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14-type cytosolic carboxypeptidase"
                     /protein_id="HEU5284958.1"
                     /translation="MTVTISSAFDAGNIRVVRQDGDLIDLEIVKDHMSDFYQWFHFRL
                     AGAAGREVTLRIVNCAGSAYPHGWPDYRACLSADREEWVRVSGTSYADGVLTMKLMPP
                     TDCVWLAYFAPYSMERHHDLVTAIAALDGVRYSSLGKSLDGQDIDCLTIGEGDLNAWL
                     YARQHPGETMAEWWMEGALEKLCDPEDPVARVLRRECTFRVIPNMNPDGSRRGHLRTN
                     AAGVNLNREWHAPSADKSPEVLCVRDAMDRSGVDFAMDVHGDEAIPANFLAGFEGIPS
                     ITERQKGLFDKFGETLERLSPDFQRAQGYEIAAP"
     gene            complement(1017..2312)
                     /locus_tag="VFU20_00440"
     CDS             complement(1017..2312)
                     /locus_tag="VFU20_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5284959.1"
                     /translation="MDRRQFLATAAVGGAATLGIAYPREAPAAPAAAFPDRSSSWDEI
                     KRQFEIKPGIVQMSSFYLASHPRPVREAIERHRRGLDQESHGYIEENVGRLERAVREA
                     AASYMRVHPDELAFTDSTTMGLGLIYSGMKLKPGQEVLTDTRDHIVTTVSASNGARRS
                     GASFRQAPLYSDPARVSAEEVVDNVRSNLRPNTRLLAITWVHSGTGVRMPVRRIAEAV
                     RAHNRGRPADERTLLALDGVHGFGVEDVAIPELGVDFFIAGTHKWLTGPRGTGLVWAT
                     EDSWRFVEPTIPTFDPMWRPPPLERMPLAAVHTPGGFHSFEHRWALAEAFRFHSGIGK
                     DRVARRIHHLNSLAKTGMAKLAKVRIHTPMSADLSAGIIAFEVAGRTPAEVVQRLHRQ
                     GIIASVTPGFYRPALARVAPSLLTTEADVERTVRAIAAL"
     gene            2461..>2981
                     /locus_tag="VFU20_00445"
     CDS             2461..>2981
                     /locus_tag="VFU20_00445"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEU5284960.1"
                     /translation="MLFDRPSPKRRMVRRLAAIGLAAAAAGAAAQTVPIIQPGAPGQP
                     AVPISADQATRIADTRFSPDDVRFMQDMIAHHQQAIDMAALVKGRTNRQAIIDVARRI
                     DASQADEMGFMRGWLRERRQAKLAPAGAHAVHSAGTGGAGHAIPGMATPEQMAALAAA
                     KGTAFDRLFLERM"
BASE COUNT          449 a         1021 c         1061 g          450 t
ORIGIN      
        1 gggcgcggcg atctcgtagc cctgcgcccg ctggaagtcc ggcgacaggc gctcgagcgt
       61 ttcgccgaac ttgtcgaaca ggcccttttg gcgctcggtg atcgagggaa tgccttcgaa
      121 gccggcgagg aagttggccg ggatcgcctc gtcgccgtgg acgtccatcg cgaaatcgac
      181 cccggagcgg tccatcgcat cgcgcacgca cagcacctcg gggctcttgt ccgcgctcgg
      241 cgcgtgccac tcgcggttga ggttcacccc ggccgcgttg gtccgcaggt gcccgcggcg
      301 cgacccgtcg gggttcatgt tggggatcac ccggaacgtg cactcgcggc gaagcacccg
      361 ggcgaccggg tcctcggggt cgcagagctt ctcgagcgcg ccttccatcc accattcggc
      421 catcgtctcg cccgggtgct ggcgggcgta gagccaggcg ttcagatcgc cctcgccgat
      481 cgtcaggcag tcgatgtcct ggccgtccag cgacttgccg agcgagctgt accggacgcc
      541 gtcgagcgcc gcgatcgcgg tcaccagatc gtggtgccgc tccatcgaat aaggcgcgaa
      601 ataggccagc cacacgcagt cggtcggcgg catcagcttc atcgtcagga cgccgtcggc
      661 gtagctggtg ccggaaaccc gcacccattc ctcgcggtcg gccgacaggc aggcgcgata
      721 gtcgggccag ccgtgcgggt aggccgagcc ggcgcaattg acgatgcgca gggtcacctc
      781 gcggccggcg gcgccggcga ggcggaagtg aaaccattgg tagaaatcgg acatatggtc
      841 cttgacgatc tcgagatcga tcaagtcgcc gtcctgcctg acgacgcgaa tattgcccgc
      901 gtcgaacgcg ctcgaaatgg tgacggtcat ggagccttct ggaaaaccgg ggtgggtcag
      961 cgcggcctga taatgcgatg ggactcgcgt gggaagcccc gcgggcggtc cagccttcag
     1021 agggcggcga tcgcccggac cgttcgctcg acgtccgcct cggtggtgag caggctgggc
     1081 gcgacgcggg ccagggccgg gcggtagaaa ccgggcgtga cgctggcgat gatcccctgc
     1141 cggtggagcc gctggacgac ttcggccgga gtccggccgg cgacctcgaa cgcgatgatc
     1201 ccggccgaca gatcggcgct catcggcgtg tggattcgaa ccttcgcaag cttcgccatc
     1261 cccgtcttgg ccaggctgtt caaatggtgg attcgcctcg cgacccggtc cttgccgatg
     1321 cccgagtgga accggaacgc ctcggccagc gcccagcggt gctcgaagct gtggaagccg
     1381 ccgggcgtgt gaaccgcggc gagcggcatc cgctcgagcg gcggcgggcg ccacattgga
     1441 tcgaaggtcg ggatggtcgg ctcgacgaac cgccaggagt cctcggtcgc ccagacgagg
     1501 ccggtgccgc gcggaccggt cagccatttg tgggtcccgg cgatgaagaa gtcgacgcca
     1561 agctcgggga tggcgacgtc ctcgaccccg aagccgtgga ctccgtcgag cgcgagcagg
     1621 gtccgctcgt cggccggcct gccgcgattg tgcgcgcgca cggcctcggc gattcggcgg
     1681 accggcatcc tcaccccggt ccccgaatgg acccaggtga tcgccagcag ccgggtgttg
     1741 gggcgcaggt tgctgcggac gttgtcgacg acctcctcgg cgctgaccct cgccgggtcg
     1801 gagtagagcg gcgcctggcg gaacgacgcg ccggagcgcc gggcgccgtt gctggccgac
     1861 accgtcgtga cgatatggtc gcgggtgtcg gtcaggactt cctggccggg cttcagcttc
     1921 attcccgaat agatgaggcc gaggcccatg gtcgtgctgt cggtgaaggc gagctcgtcc
     1981 ggatggaccc gcatgtagct cgccgcggcc tcgcggaccg cgcgctccag cctcccgaca
     2041 ttctcctcga tatagccgtg cgattcctgg tcgaggccgc ggcggtggcg ctcgatcgcc
     2101 tcgcggaccg gcctggggtg cgaggcgagg tagaagctcg acatctggac gatgcccggc
     2161 ttgatctcga actggcgctt gatctcgtcc cacgagctgg accggtccgg gaacgccgcg
     2221 gccggcgccg ccggagcctc ccgcgggtaa gcgatgccga gagtcgcggc gccgccgacg
     2281 gccgccgtgg cgagaaattg cctgcgatcc ataactgttt cccctgactt ggcggcgtta
     2341 ccaaaccgca ggcggattgg cgaccgacag cgagcgccgc cgggccgccg ccgacgctgg
     2401 cgcgcagttg agtcctcggc tagccatcct ggccaacgac cgcccaggag aagctcccga
     2461 atgctgttcg accgtccatc accgaagcgc cgaatggtcc gccggcttgc cgccatcggc
     2521 ctcgcggccg cggccgccgg cgcggcggcg cagaccgtgc cgatcatcca gccgggggcg
     2581 cccgggcagc cggcggttcc gatcagcgcc gaccaggcga cccgcatcgc cgacacccga
     2641 ttctcgccgg acgacgttcg cttcatgcag gacatgattg cccatcacca gcaggcgatc
     2701 gacatggcgg cgctggtcaa gggacgcacc aaccggcagg cgatcatcga cgtcgcccgc
     2761 cggatcgacg cgtcccaggc ggacgagatg ggcttcatgc gcggctggct ccgcgagcgg
     2821 cggcaggcca agctcgcccc agccggcgcc cacgcggtgc actcggccgg gaccggcggc
     2881 gccggccacg cgattccggg gatggcgacg cccgagcaga tggccgcgct tgcggccgcc
     2941 aagggaaccg cattcgaccg cttgttcctc gagcggatga t
//
LOCUS       DASTZO010000020         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1228689,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000020 DASTZO010000000
VERSION     DASTZO010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228689"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VFU20_00450"
     CDS             <1..270
                     /locus_tag="VFU20_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HEU5284961.1"
                     /translation="MRGLDLGLERFKFFPAASSGGIPALKALSAPFGDARFCPTGGVT
                     AETASDWLALDAVLCVGGSWLLRPGETDLAAVARRARDAAALGVR"
     gene            complement(301..376)
                     /locus_tag="VFU20_00455"
     tRNA            complement(301..376)
                     /locus_tag="VFU20_00455"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(341..343),aa:Ala,seq:ggc)
     gene            complement(439..759)
                     /locus_tag="VFU20_00460"
     CDS             complement(439..759)
                     /locus_tag="VFU20_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02300 family protein"
                     /protein_id="HEU5284962.1"
                     /translation="MVKPEWGAKRTCPKCSTRFYDLGNDDPVHCIECGNEWVPEPILK
                     SKQPLPFEPAAPVAKEPAAETDLEAEDLAIDEDEEPSADDEVDLTTGDDDLGVETAPD
                     EDEA"
     gene            923..1567
                     /gene="cmk"
                     /locus_tag="VFU20_00465"
     CDS             923..1567
                     /gene="cmk"
                     /locus_tag="VFU20_00465"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU5284963.1"
                     /translation="MVIAVDGPAASGKGTIARALASHFKLPHMDTGMLYRAVALNLWR
                     WGGDLSSEFEALRACDVAAVDPDDPELRSEPVSSIASRISAYPSVRAALLDRQRQFAG
                     QPGGAVLDGRDIGTVVAPDAAAKLFVTASVEVRAQRRVRELLERGLPAHYDDVLTDIR
                     ARDERDTRREAAPLKQARDADLLDTSTLDVDQAIAAAIRLVEQRLASRLPLNQK"
     gene            1641..1835
                     /locus_tag="VFU20_00470"
     CDS             1641..1835
                     /locus_tag="VFU20_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284964.1"
                     /translation="MPDYKLYCLDGARRIARAPDVISAESDEQAIAAARGLGMASACE
                     VWQGRRLVATIPAGGAKPRR"
     gene            complement(1843..>2301)
                     /locus_tag="VFU20_00475"
     CDS             complement(1843..>2301)
                     /locus_tag="VFU20_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU5284965.1"
                     /translation="WEAVAAGTVQAHLRQLPGPENMMGAIKFGFNNDLGIYLHDTPHK
                     NLFGERQRTFSLGCVRLEDAGRLGAWLLAREPVPPSDRPEQSVRLPRPVPVYISYITL
                     SADNGRLAFVDDVYGRDSKAGTGLAALEPLTPLDGTAPNPLAAASPATPH"
BASE COUNT          319 a          798 c          822 g          362 t
ORIGIN      
        1 atgcgcgggc tcgacctcgg gctcgagcgg ttcaaattct tccccgcggc aagctcgggc
       61 ggaatcccgg cgctgaaggc gctcagcgcg ccgttcggcg atgcccgctt ctgcccgacc
      121 gggggcgtca ccgccgagac cgcgagcgat tggctggcgc tggatgcggt gctgtgcgtc
      181 ggcggcagct ggctgctccg ccccggcgaa accgacctcg cggccgtggc ccggcgggcg
      241 cgggatgcgg ccgcgctagg cgtccgctag cggccggatt tccgtttgtt tatggacgga
      301 tggtggagcc gagggggatc gaacccctga ccttcgcaat gccattgcga cgctctccca
      361 gctgagctac ggccccgtcc gaacacgcgc ctttaggcga agttcaaccc cctgaaaact
      421 cgaaaaatcg atcgcggcct aggcctcgtc ctcgtcgggc gcggtctcca ccccgaggtc
      481 gtcgtcgccg gtcgtgaggt cgacctcgtc gtcggccgac ggctcctcgt cctcgtcgat
      541 cgccagatcc tcggcttcga ggtcggtctc cgccgccggc tccttggcga ccggcgcggc
      601 cggctcgaac ggaagcggct gcttggactt gaggatcggc tcggggaccc attcattgcc
      661 gcattcgatg caatggaccg gatcgtcgtt gccgaggtcg tagaagcggg tcgagcactt
      721 ggggcaagtg cgcttggcgc cccattcggg cttcaccata ttgagtttta ctccagtttc
      781 gggttcggtc cggcaggtcc aggatatggc cgtggggagg cccggcaccc gtgcggcggg
      841 cgccttgcca taggcttgga cccctgtcaa agcgcatcgc ttgagcgagc atcccgacaa
      901 cgaaaaggcc gcgccggagc cgatcgtcat cgccgtcgac ggccccgcgg cgagcggcaa
      961 ggggaccatc gcccgggcgc tcgcgagcca tttcaagctg ccgcacatgg acaccgggat
     1021 gctgtaccgc gcggtcgccc tgaacctgtg gcggtggggc ggcgacctgt cgagcgagtt
     1081 cgaggcgctt cgcgcctgcg acgtcgcggc cgtcgacccg gacgaccccg agctgcgcag
     1141 cgagccggtg tcgagcatcg cctcgcggat ctcggcctat ccgtcggtcc gggccgcgct
     1201 gctcgaccgg cagcgccagt tcgccggcca gccgggcggg gcggtgctcg atgggcgcga
     1261 catcggcacg gtggtcgccc cggacgcggc cgccaagctg ttcgtcaccg cttcggtcga
     1321 ggtgcgcgcc caacggcggg tgcgcgagtt gctcgagcgc gggctgccgg cgcattacga
     1381 cgatgtcctg accgatatcc gcgcccgcga cgagcgcgac acccggcgcg aagccgctcc
     1441 gctcaagcag gctcgcgacg ccgacctgct cgacaccagc acgctcgacg tcgaccaggc
     1501 gatcgccgcg gcgatccggc tggtcgagca gcgcctcgct tcgcgcttac ccttgaatca
     1561 aaagtaattt ttccaattgt cgggcggcgg actcggggaa tccgaccgtt tcggctatgg
     1621 ttcgacgccg ggggaggcgg atgcccgact acaagctcta ttgcctcgac ggggcgaggc
     1681 gcatcgcgcg cgcgcccgac gtcatcagcg ccgagagcga cgagcaggcc atcgccgcgg
     1741 cgcgcggcct cggcatggcg agcgcctgcg aggtctggca ggggcggcgg ctggtcgcga
     1801 ccatacccgc gggcggcgca aaaccgcgtc ggtgagcgac tctcagtgcg gcgtcgccgg
     1861 gcttgccgcg gcgagcgggt tgggcgccgt cccgtcgagc ggagtcagcg gctcgagcgc
     1921 cgccagcccg gtcccggcct tcgagtcgcg gccgtagacg tcgtcgacga acgccagccg
     1981 gccgttgtcc gcggaaagcg tgatgtagct gatgtagacc ggcaccggcc tgggcagccg
     2041 gaccgactgc tcgggccggt cggacggcgg caccggctcg cgcgccagca gccaggcgcc
     2101 gagccgcccg gcgtcctcga gccgcacgca gccgaggctg aacgtccgct gccgctcgcc
     2161 gaacagattc ttgtgcggcg tgtcgtgcag gtaaatgccg aggtcgttgt tgaagccgaa
     2221 cttgatcgcc cccatcatgt tctcggggcc cggcagctgg cggaggtgcg cttgcacggt
     2281 gcccgcggcg acggcttccc a
//
LOCUS       DASTZO010000021         9156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_210306,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000021 DASTZO010000000
VERSION     DASTZO010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9156
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210306"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VFU20_00480"
     CDS             <1..561
                     /locus_tag="VFU20_00480"
                     /inference="COORDINATES: protein motif:HMM:NF012486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosinase family protein"
                     /protein_id="HEU5284966.1"
                     /translation="ALLRQFATIMPTLPTAEDVKLAMDEVLYDTPPFSSSPFVVGFRN
                     RIEGWVTQRGDPRVKTPGSQLHNRVHLWVGGSMVPMTSPNDPVFFLHHCFIDKIWADW
                     QAANEALWREQGYPGTAPQYNPVSGGPVGHNLDDRLTPWTRTISEVLSSSALGYHYEA
                     AVNESLFAVTRRISPFAVERSPFWAD"
     gene            complement(593..1129)
                     /locus_tag="VFU20_00485"
     CDS             complement(593..1129)
                     /locus_tag="VFU20_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HEU5284967.1"
                     /translation="MQFALQPHPASPPGGPPFKVWASVDHVASLGAVATTNIWFGVGA
                     PAGRFVIPKLSKPWRADELWRTTCFEAFLRPAGAESYREWNFAPTGNWAAYDFQQYRE
                     GMTRADVGSPPYIRMEDNFTWWTVGATIAADAGRQFALGLSAVLEEKDGTKSYWALAH
                     PAEKPDFHHPDCFAAQLA"
     gene            1175..2383
                     /gene="tyrS"
                     /locus_tag="VFU20_00490"
     CDS             1175..2383
                     /gene="tyrS"
                     /locus_tag="VFU20_00490"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU5284968.1"
                     /translation="MTSYKSSLLRLLDERGYIHQVTDAAALDALAEKDTVTAYIGFDA
                     TAPSLHVGSLVQIMLLRRLQQAGHKPVVLMGGGTTKVGDPSGRDESRKLLTAEDIAAN
                     IASIRRAFEKLLKFGDGPTDAILLDNAEWLDGLAYVPFLREVGRHFTINRMLTFDSVK
                     LRLEREQPLTFLEFNYMILQAYDFLELSRRVGCRLQMGGSDQWGNIVNGIELGRRVDG
                     TELYGLTTPLVTTADGAKMGKTAQGAVWLNADLLSPYDYWQFWRNTDDADVGKFLRRF
                     TDLPLDEIRMLETLQGAELNAAKVALATAATAMLHGDDAARTAQETAKATFEGGAGED
                     LPTLSVGDGVNIAHALTQLGFTPSNKEAKRKIAEGAVRLNDEVVGDPGLVVTEGKLSL
                     GRKKHALLVR"
     gene            complement(2385..2807)
                     /locus_tag="VFU20_00495"
     CDS             complement(2385..2807)
                     /locus_tag="VFU20_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284969.1"
                     /translation="MLDEQRPSLDAIFGPDSYVIEETSPYSAILKTAIADFEFSDDPR
                     DFVSASITSVPAVDLIQSPLDIWMRFLDEEIPAQRRSDPYQEQLANELKWIAVIVERI
                     LKDADRRREAASFVNGYNRAYNDWASGKGSWAEPDFND"
     gene            complement(2840..4894)
                     /gene="recG"
                     /locus_tag="VFU20_00500"
     CDS             complement(2840..4894)
                     /gene="recG"
                     /locus_tag="VFU20_00500"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU5284970.1"
                     /translation="MRPEILNPLFAEAEVLKGVGPGLAKALKRIGLTRVIDLIYHLPS
                     GTIERVWARAIGHELVGRIVIVEVTPFETRAAQGRAPFRVFASDADGNTLTLVYFNHP
                     GWAKKSLPLGEKRLVSGKLEAYGQELQIIHPEVTAPGKDADLPLREPVYGLTEGVTNR
                     RMRELAAAALERAPELPEWIEPSLAAREGWRPWRAALAEAHAEPLAGQNRRRLAYDEI
                     FANQLALMLLRQSARRKRGVPLTGDGRLIDRLQLPYTLTGAQRRVIGEIRGDMAQGVP
                     MLRLLQGDVGSGKTLVALMAMLIAVEGGAQAALLAPTEILARQHHATLLAQLDSLGVR
                     VAILTGREKGRARDSVYMGLGDGSIDILVGTHAIFQEKVAYRNLGLAVIDEQHRFGVS
                     QRLLLSAKAERPPHLLVMTATPIPRTLTLTQYGEMDVSRIDEMPPGRTPVETRVISDE
                     RLGEVIDGLGRHLAGGGQAYWVCPLVEESDKIDSAAAEQRAEVLRLRFGDRIGLVHGR
                     MKGPEKDSVMAAFASGKLAVLVATTVVEVGVDVPNATLMVVEGAERFGLAQLHQLRGR
                     VGRGAGKSICLLVRGPNLSETARARLALMRETNDGFRIAEEDLRLRGPGEILGTRQSG
                     EAGFRVASAEEVAELATVAQSDAQLLLDRDGGLAGPRGQAARTCLYLFERDQAVGLIR
                     SG"
     gene            complement(4897..5793)
                     /locus_tag="VFU20_00505"
     CDS             complement(4897..5793)
                     /locus_tag="VFU20_00505"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5284971.1"
                     /translation="MFAWDDIAVFLALYRERTTGRAATALGCSQPTVVRRIAALEQAL
                     GLILFDRTPTGLVPTDAAHRLLARAERVERAACEFTAEVDQLTGSGLDILKLTFIDHF
                     ERLLLPVLREFRTRWPGVQTQLLASDRIYDLARGEADIGIRGRLRPAGDEVVVRQLPP
                     NGWTIYASSSSKPDEHPHSPAEVADYRLALIEGAPSKLPVYQWLERLAANGPTPIRCT
                     NYGALRSAIASGAAVSALPCSAGDGDPDVVPCFPPIEEFDVEIFLVARRAILRRPPAR
                     DLFDTIENYFRTNPALLTGERG"
     gene            5974..6540
                     /locus_tag="VFU20_00510"
     CDS             5974..6540
                     /locus_tag="VFU20_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284972.1"
                     /translation="MPEPTLEDVRAATSRFQDVKVALAEGYVRDPDDVCVTADMVGLP
                     ASDGAMGVHYIRMDLLGITGPPNPRVNGAGTHTDFLKPGVLLYEPQADGSLVLVAVEN
                     LVFVESWHKAGNTRLPAYQNVAYDLMADNPATPIDEAHMFEPHYDRHVWLYRVNPRGI
                     YTQFNPAVSCAHHKGAKHHHPVAVSMEK"
     gene            6594..6860
                     /locus_tag="VFU20_00515"
     CDS             6594..6860
                     /locus_tag="VFU20_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HEU5284973.1"
                     /translation="MPPADDIKRLRYRAWHRGTREADMLVGGFFDAHHRQWGPLERAL
                     FADLLDEQDVDILAWAHGTAEPPARFEGPMLEALKRRDYIRVAR"
     gene            6857..>9156
                     /locus_tag="VFU20_00520"
     CDS             6857..>9156
                     /locus_tag="VFU20_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5284974.1"
                     /translation="MSELQRVLGASEPLTLAGIPTGFLPLLAADLARAAHGQSARAVL
                     VAADEAAMRALADTVPVFAPDVEVLTLPAWDCLPYDRASPALRVMAERLATLNALQAK
                     RKGPQLLVATANAATQRLLTPFRIRQLTRRMEIGERVEREALIAQLNALGYQRADAVA
                     EAGEYAVRGSLIDLFPAGEETALRLDFFGDEVESIRRFDPADQRSTDKAQAFTLLPAS
                     EALLDEVSIRRFRSRYRETFGAGATGDPLYQALSEGRRMSGMEHWLPLLEESLSTLFD
                     HLGELDIVVRDSGTDGALAARREAIADYHSNRTRAMAAEPGSYRPLEPGLLYLPKEEW
                     DAAVAGRPVHLASPFPEPESARTVPFEVEPARDFAPERAQQANVYEAVAAHVQQLRKA
                     GRKVVLASYTRGARERLHGLLEDHGLKSLKLVDSWADALAAKGSAALMVLPLDHGFTA
                     ADVAVLTEQDMLGDRLVRRRRKRKAAAAFLEELATLSPGDLVVHSDHGVGRYEGLTSI
                     KVGNSPHDCVALEYAGGNRLYVPVENIELLSRYGSESEGVSLDRLGGEAWQRRKSRMK
                     QRIREIAGELIKTAALRATRPGVVAEPDDSAFPQFVDRFPYEETDDQDRAIEEVLADL
                     ESGKPMDRLVCGDVGFGKTEVALRAAFVVAMSGLQVALIGPTTLLARQHYAQFAERLQ
                     GFPIQLGRLSRLVPAAEAKKTKEGLASGQVDIVVGTHALLAKGLKFKRLGLVIVDEEQ
                     HFGVAHKERLKALRADVHVLTLTATP"
BASE COUNT         1515 a         3195 c         3004 g         1442 t
ORIGIN      
        1 gcgctgctgc gccagtttgc aacgatcatg ccaacgctgc cgactgcaga ggatgtcaaa
       61 ttggcgatgg acgaagtgct ttatgacact cctcctttca gcagcagccc gttcgtcgtc
      121 ggcttccgca atcgaattga aggttgggtg acgcaacgcg gggatccgcg cgtcaaaacc
      181 cccggttccc aactccataa ccgggtccat ctctgggtcg ggggatcgat ggttccgatg
      241 acctcgccta acgaccccgt cttcttcctt caccattgct tcatcgacaa gatctgggcc
      301 gattggcaag cggcgaacga ggcgctgtgg cgagaacaag gttacccggg cactgcccct
      361 caatataatc cagtcagcgg cgggcccgtc gggcacaacc tcgacgacag gctgactcca
      421 tggacccgga cgattagcga agtccttagt tcgtcggctc ttggctacca ctatgaggcc
      481 gctgtcaacg agtcgctgtt tgcagtcact cggcgcattt caccctttgc agtcgagcga
      541 tcgccgttct gggcagattg acgcggctca ggaggctgcc gttcggaggt gcttaggcaa
      601 gctgcgcggc gaagcaatcg ggatggtgga aatcgggctt ttccgccggg tgcgcgagcg
      661 cccaatagga ctttgtcccg tccttctcct cgagcaccgc cgacaggccg agcgcaaact
      721 gccggccggc atcggcggcg atggtcgccc cgaccgtcca ccaggtgaaa ttgtcctcca
      781 tccggatata gggcggcgag ccgacatcgg cccgagtcat gccctcccgg tattgctgga
      841 aatcataggc cgcccaattg ccggtcggag cgaaattcca ctcgcggtac gattcagcgc
      901 cggcgggcct caggaacgcc tcgaagcagg tcgtccgcca caattcgtcg gcgcgccacg
      961 gcttggacag tttgggaatc acgaaccggc cggcgggagc gccgaccccg aaccagatgt
     1021 tggtcgtcgc gaccgcgccg agcgaggcga cgtggtcgac gctcgcccac accttgaacg
     1081 gcgggccgcc gggcggcgac gcgggatggg gctgaagggc gaactgcacc gcatcgcagt
     1141 agcagcgtgg aaagcatgac gctaagccgc gctgatgacg tcctacaaat cctccctcct
     1201 gcggctgctc gacgagcgcg gctacatcca ccaggtgacc gacgccgctg cgctcgacgc
     1261 cctggcggag aaggacacgg tcaccgccta catcggcttc gacgcgaccg cgccgagcct
     1321 ccacgtcggc agcctggtgc agatcatgct gctccgccgg ctccagcagg ccgggcacaa
     1381 gccggtggtg ctgatgggcg gcggcacgac caaggtcggc gacccgtcgg ggcgcgacga
     1441 aagccgcaag ctgctgacag ccgaggacat cgccgccaac atcgcctcga tccgccgggc
     1501 gttcgagaag ctgctcaagt tcggcgacgg cccgaccgac gcgatcctgc tcgacaatgc
     1561 cgagtggctc gacggcctcg cctacgtgcc cttcctgcgc gaggtcggcc gccatttcac
     1621 catcaaccgg atgctgacct tcgactcggt caaattgcgg ctcgagcgcg agcagccgct
     1681 gaccttcctc gagttcaact acatgatcct ccaggcctac gacttcctcg agctgtcgcg
     1741 gcgcgtcggc tgccggctgc agatgggcgg ctccgaccag tggggcaata tcgtcaacgg
     1801 gatcgagctc gggcggcggg tcgacgggac cgagctctac gggctgacga cgccgctggt
     1861 cacgaccgcg gacggcgcca agatgggcaa gaccgcgcag ggcgccgtgt ggctcaacgc
     1921 ggacctgctc agcccctacg attactggca attctggcgg aacaccgacg acgccgacgt
     1981 cggcaaattc ctgcgccggt tcaccgacct gccgctcgac gagatccgga tgctggaaac
     2041 actgcagggc gccgagctaa acgcggcgaa ggtcgcgctc gcaacggcgg cgacggcgat
     2101 gcttcacggc gacgacgccg ctcgcaccgc tcaggagact gcaaaagcca ccttcgaagg
     2161 gggcgctggg gaggatttgc cgaccttgag cgtcggcgac ggggtcaaca tcgcccatgc
     2221 gctgacccag ctcggcttca ccccgtcgaa caaggaagcc aagcgcaaga tcgccgaggg
     2281 cgcggtgcgg ctcaacgacg aggtggtcgg cgatccgggc ctcgtggtca ccgaaggcaa
     2341 gctcagcctc ggccgcaaga agcacgcgct gctagtccgc tagcctagtc attgaagtcc
     2401 ggctcggccc agcttccctt accgctggcc cagtcgttgt aggctcgatt gtagccgttg
     2461 acgaaggacg cggcctctct cctcctgtcc gcgtccttta aaatcctctc aactatcacc
     2521 gcgatccatt tgagctcgtt tgctaattgc tcttggtacg ggtcgcttcg tcgctgagct
     2581 gggatttcct cgtcgagaaa cctcatccag atgtccagtg gcgactgaat taggtcgacc
     2641 gccgggacgc ttgtgatgga ggccgaaacg aagtctcgcg ggtcgtcgct gaactcaaag
     2701 tcagcaatcg ccgtcttcaa gatcgcggaa tatgggctcg tttcttcgat gacataactg
     2761 tcagggccga agatggcgtc aagggagggg cgctgctcgt caagcatgat ggcgacatgg
     2821 ggtcgtccgg acatttcagt cacccgctcc taatcagccc aaccgcctgg tcgcgctcga
     2881 acagataaag gcaggtgcgc gccgcctgcc cgcgcggccc cgcgaggccg ccgtcgcggt
     2941 cgagcagcag ctgcgcgtcg ctctgcgcga ccgtcgccag ctcggcaacc tcctcggccg
     3001 acgcaacccg gaagcccgcc tcgcccgatt gccgggtgcc gaggatctcg cccggcccgc
     3061 gcagccgcaa atcctcctcg gcaatccgga aaccgtcgtt ggtctcgcgc atcaatgcga
     3121 ggcgggcccg ggcggtctcc gacaggttcg gcccacgcac cagcaggcag atgctcttgc
     3181 ccgccccgcg cccgacccgc ccgcgcagct ggtggagctg ggcgagcccg aaacgctcgg
     3241 cgccctcgac caccatcaac gtcgcgttgg gaacgtcgac gccgacttcg accacggtgg
     3301 tcgcgaccag caccgccagc ttgccggaag cgaacgccgc catcaccgaa tccttctccg
     3361 gccccttcat ccgcccgtgg accagcccga ttcggtcgcc gaatcgcagc cgcagcacct
     3421 cggcgcgctg ctcggcggcg gcgctgtcga tcttgtcgct ttcctcgacc aaggggcaca
     3481 cccaataggc ctggccgccg ccggccaggt gccgcccgag cccgtcgatc acttcgccca
     3541 agcgctcgtc gctgatcacc cttgtctcga ccggcgtccg gcccggcggc atctcgtcga
     3601 tccggctgac gtccatctcg ccgtactgag tcagagtcag ggtgcgcggg atcggggtcg
     3661 cggtcatcac cagcaggtgc ggcgggcgct cggccttggc ggacagcagc agccgctgcg
     3721 acaccccgaa ccggtgctgc tcgtcgatca ccgccagccc gagattcctg taggcgacct
     3781 tctcctggaa gatcgcgtgg gtgccgacga ggatgtcgat cgacccgtcg ccgagcccca
     3841 tgtagaccga atcgcgcgcc cgccccttct cgcggcccgt caggatcgcc acccgcaccc
     3901 cgagcgaatc gagctgggcg agcagcgtcg catggtgctg gcgggcgagg atctcggtcg
     3961 gcgccagcag cgccgcctgg gcgcccccct cgaccgcgat cagcatcgcc atcagagcga
     4021 ccagcgtctt gcccgagccg acgtcgccct gcagcaagcg cagcatcggc acgccctgcg
     4081 ccatgtcgcc gcggatttcc ccgatcaccc gccgctgggc gccggtcagc gtgtagggca
     4141 gttgcagcct gtcgatcagc cggccgtcgc cggtcagcgg aacgccgcgc ttgcgccgcg
     4201 ccgactggcg cagcagcatc agcgccagct ggttggcgaa aatctcgtca taggcgagcc
     4261 ggcggcggtt ctggcccgcc agcggctcgg catgggcttc ggcaagcgcc gcccgccacg
     4321 gccgccagcc ctcgcgcgcg gcgaggctcg gctcgatcca ttcgggcagc tcaggcgcgc
     4381 gttcgagcgc cgcggcggcg agctcgcgca tccgccggtt ggtgactccc tcggtcaggc
     4441 cgtagaccgg ctcgcgcagc ggcagatcgg catccttgcc cggcgccgtc acctcgggat
     4501 ggatgatctg cagctcctgc ccgtaggcct cgagcttgcc cgacacgagg cgcttctcgc
     4561 ccagcggcag gctcttcttc gcccagccgg gatggttgaa atagaccaaa gtcagagtgt
     4621 tgccgtcggc gtcggacgcg aacacacgga acggcgcccg cccctgcgcg gcgcgggtct
     4681 cgaacggggt cacctcgacg atcacgatcc ggccgaccag ctcgtgaccg atcgcccgcg
     4741 cccaaacccg ctcgatcgtc ccgctcggca gatggtagat gaggtcgatg acccgggtca
     4801 ggccgatccg cttgagcgcc ttggccagcc ccggcccgac gcctttcagc acctcggcct
     4861 cggcgaacaa gggattgagg atctcagggc gcatcgtcag ccgcgctcgc cggtcagcag
     4921 ggcggggttt gtcctgaagt agttttcgat cgtatcgaaa aggtcgcgcg ccggcggccg
     4981 ccggagtatc gcgcgccggg cgaccaggaa gatctcgacg tcgaactcct cgatcggcgg
     5041 aaagcagggg acgacgtcgg ggtcgccatc gcccgcgctg cacggcagcg ccgaaacagc
     5101 ggccccggag gcaatggcgg acctcaaggc tccatagttg gtgcagcgga tcggcgttgg
     5161 gccgtttgcc gccagccgct ccagccactg gtagaccggc agtttcgaag gcgcgccttc
     5221 aatcagcgct agccggtaat cggccacttc ggcaggcgaa tgcggatgtt cgtccggttt
     5281 gctgctcgac gatgcgtaga tcgtccatcc attcggcggc agctgacgca ccacgacctc
     5341 gtcgccggcc gggcgcagcc ggccgcgaat gccgatgtcc gcctctcccc gcgcgaggtc
     5401 gtaaatccgg tcgcttgcga gcaactgcgt ttggacgccc ggccaccgcg tgcggaattc
     5461 gcgcagcacc ggaagcagca agcgctcgaa atgatcgatg aatgtgagct tgaggatgtc
     5521 gagaccgctg ccggtaagct gatcgacctc ggccgtgaat tcgcacgccg cccgttccac
     5581 gcgctcggcg cgggcaagca gtcgatgggc cgcgtcggtc gggacgagac cggttggagt
     5641 ccggtcgaac aggatgagcc cgagcgcctg ctcgagcgcg gcaatgcggc gaacgacggt
     5701 cggctggctg catccaagcg ccgtcgcggc gcggccggtc gtccgctcgc gataaagcgc
     5761 caggaaaacc gcgatgtcgt cccacgcgaa catcccaacg agctattcac atttgaatac
     5821 ccccttgcaa gcctttcggt tgaacccctt tcgacgcggg cgtaggcgaa tgccggacag
     5881 gagaccaaga ccatgatcaa gaccgtgctc gggcttgcgg cagcatccgc ggccaccttt
     5941 gcttctgcaa tgcctgaatc gccgcaagcg agattgcccg agccgacgct cgaggacgtg
     6001 cgcgccgcga ccagccgctt ccaggacgtc aaggtggcgc tggccgaagg ctatgtccgc
     6061 gaccccgacg acgtctgcgt cacggccgac atggtcggct tgccggcaag cgacggcgcg
     6121 atgggcgttc attatatccg gatggacttg ctcggcatca ccgggccgcc gaacccgcga
     6181 gtcaacgggg ctggcaccca caccgatttc ctcaagccgg gagtcctgct ctacgagccg
     6241 caggcggacg gttcgctggt cctggtcgcg gttgagaacc tcgtgttcgt cgagtcctgg
     6301 cataaggctg gaaacacccg gctgccggcc tatcagaatg tcgcctacga tttgatggcg
     6361 gacaatccag cgactccgat cgacgaggcg cacatgttcg agccgcatta cgaccgccat
     6421 gtctggctgt atcgcgtcaa cccgaggggg atctacacgc agttcaaccc ggcggtcagc
     6481 tgcgcgcatc acaagggagc caagcaccac catccggttg ccgtgtcgat ggagaaatag
     6541 gcgccgaagc ggccggtctt ccctcgcggg cggctcttgc ctagagccgc gcggtgcccc
     6601 cagccgacga tatcaagcgc ttgcgctacc gagcgtggca tcgcggcacg cgcgaggccg
     6661 acatgctggt cggagggttc ttcgacgctc accaccggca atggggtccg ctcgagcgcg
     6721 cattgttcgc cgatctgctc gacgagcagg acgtcgacat cctcgcctgg gcgcatggaa
     6781 ccgccgagcc gccggcgcgg ttcgagggtc cgatgctgga ggcgctgaag cggcgcgact
     6841 atatccgggt tgctcgatga gcgagctgca gcgcgttctt ggcgccagcg agccgctaac
     6901 cctggccggc atcccgaccg gcttcctgcc gttgctggcc gccgacctgg cgcgcgccgc
     6961 gcacgggcaa agcgcccgcg ccgtgctggt cgcggccgac gaagcggcga tgcgcgcgct
     7021 cgccgacacc gtccccgtgt tcgcgcccga cgtcgaggtg ctgaccctgc cggcgtggga
     7081 ctgcctgccc tacgaccgcg cgtcgcccgc gctccgggtg atggccgagc ggctggcgac
     7141 gctcaacgcg ctgcaggcga agcgcaaggg cccgcagttg ctggtcgcga ccgccaatgc
     7201 cgcgacgcag cggctcctga ccccgttccg gatccgccag ctgacccgcc ggatggaaat
     7261 tggcgagcgg gtcgagcgcg aggcgctgat cgcgcagctg aacgccttgg gctaccagcg
     7321 cgccgacgcg gtcgccgagg ccggcgaata tgcggtgcgc gggtcgctga tcgacctgtt
     7381 cccggccggc gaggaaacgg cattgcggct cgacttcttc ggcgacgagg tcgaaagcat
     7441 ccgccgcttc gacccggccg accagcgctc gaccgacaag gcccaggcgt tcaccctgtt
     7501 gccggcgtcg gaagccctgc tcgacgaggt cagcatccgc cgattccgtt cgcgctaccg
     7561 cgagacgttc ggcgccggcg cgaccggcga cccgctctac caggcgctgt ccgaagggcg
     7621 gcggatgagc ggcatggagc attggctgcc gctgctcgag gaaagcctgt cgaccctgtt
     7681 cgaccatctc ggcgagctgg acatcgtcgt ccgcgacagc ggcaccgacg gagcgctcgc
     7741 cgcccggcgc gaggcgatcg ccgattatca ttcgaaccgg acccgggcga tggccgccga
     7801 gcccggcagc taccgcccgc tcgagccggg cctgctctat ctgcccaaag aggaatggga
     7861 tgccgcggtc gccgggcgcc cggtccacct cgcctccccg ttcccggagc ccgaaagcgc
     7921 gcgcacggtt cccttcgagg tcgagccggc gcgcgatttc gcgcccgagc gcgcccagca
     7981 ggccaatgtc tacgaagcgg tcgccgcgca tgtgcagcag ctcaggaagg ccggccgcaa
     8041 ggtcgtgctc gccagctaca cccgcggcgc ccgcgagcgg ctccacggcc tgctcgagga
     8101 ccatggcctc aagtcgctga agctcgtcga cagttgggcc gacgcgctcg cggccaaggg
     8161 gtccgcggcg ctgatggtgc tgccgctcga ccacggcttc accgccgccg acgtcgcggt
     8221 gctgaccgag caggacatgc tcggcgaccg gctggtccgc cgccgccgca agcgcaaggc
     8281 cgccgccgcc ttcctcgagg agctcgcgac gctttctcca ggagatcttg tcgtccactc
     8341 cgaccatggc gtcgggcgct acgaggggct gacgtcgatc aaagtcggca actcacccca
     8401 cgactgcgtc gccctcgaat atgccggcgg caacaggctc tacgtcccgg tcgagaatat
     8461 cgagctgctc agccgctacg gcagcgagtc cgagggcgtc agcctcgacc ggctgggcgg
     8521 cgaagcctgg cagcggcgca agtcgcggat gaagcagcgt atccgcgaga tcgccggcga
     8581 gttgatcaag accgcggcgc tgcgcgcgac ccgccccggg gtcgtcgccg agcccgacga
     8641 ttcggctttc ccgcagttcg tcgaccgctt cccctacgag gagaccgacg accaggaccg
     8701 ggcgatcgaa gaggtgctcg ccgaccttga atcgggcaag ccgatggacc ggctggtgtg
     8761 cggcgacgtc ggtttcggca agaccgaggt cgctctgcgc gccgccttcg tcgtcgccat
     8821 gtccgggctg caggtcgcgc tgatcggccc gaccaccctg ctcgcccgcc agcattatgc
     8881 gcagttcgcc gagcggctgc agggcttccc gatccagctc gggcggctgt cgcggctggt
     8941 gcccgcggcc gaggcgaaga agaccaagga agggctcgcg agcggccagg tcgacatcgt
     9001 cgtcggcacc cacgcgctgc tggcgaaagg gctgaagttc aagcgcctcg gcctcgtcat
     9061 tgtcgacgag gagcagcatt tcggagtcgc gcataaggag cggctgaagg ccctccgtgc
     9121 cgacgtccac gtgctgaccc tcaccgcgac gcccat
//
LOCUS       DASTZO010000022         2774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1280772,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000022 DASTZO010000000
VERSION     DASTZO010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1280772"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..568)
                     /gene="rfbC"
                     /locus_tag="VFU20_00525"
     CDS             complement(14..568)
                     /gene="rfbC"
                     /locus_tag="VFU20_00525"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEU5284975.1"
                     /translation="MLDTRPLALDGLVEIRPPRFGDERGFFSQVWSAENMAEAGLEIG
                     FVQDNHSYSAARGVLRGLHYQVPPAAQHKLVRVSRGSIFDVAVDIRPGSPTFGQWAGL
                     TLSAELWNQLLVPEGFAHGFVTLEEHTEVQYKVSAPYRPELERSIRYDDPDIAIDWPV
                     PASEIRLSARDSAAPLLSGGPTGF"
     gene            complement(561..1442)
                     /gene="rfbA"
                     /locus_tag="VFU20_00530"
     CDS             complement(561..1442)
                     /gene="rfbA"
                     /locus_tag="VFU20_00530"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEU5284976.1"
                     /translation="MKGIVLAGGSGSRLYPATLAVSKQLLPVFDKPMIYYPLGVLMLA
                     GITEILIISTPTDLPRFRELLGDGSAYGVSFSYAEQPEPNGLAEAFIIGREFVGDSPV
                     ALVLGDNIFYGAGLVEMVRQAAAQTRGATVFAYGVEDPERYGVVTFDPETGRALTIEE
                     KPAEPQSNWAVTGLYFYDNDVLDIAAGLTPSARGELEISDVNSAYLERGTLHVTRLGR
                     GFAWLDTGTHESLHDASSYVRTIEHRQGLKVMCLEEIALELGYLTPDQVRARADQLGK
                     TGYADYIRRRAKEIAGA"
     gene            complement(1640..>2774)
                     /gene="dnaA"
                     /locus_tag="VFU20_00535"
     CDS             complement(1640..>2774)
                     /gene="dnaA"
                     /locus_tag="VFU20_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU5284977.1"
                     /translation="PIVREVRVGAAADAPRPSPLLILEEAPEAPPAARDPSTPNFDPR
                     YRFETFIVGKANEVAATAARTLAGTGAVGFNPLFIHGGTGRGKTHLLHAIGHGFLAAN
                     PGKRVVSMSAEKFMVEFIRALKANDTIGFKGRLRNADLLLIDDVQFIAGKDSTQEEFF
                     HTMNEIITAGRRLVITSDRAPQDLDGVAPRILSRLSWGLVADINAADFELRFNIIQAK
                     LGALPGVEMPRNVVDFLARRVTSSVRELEGALNRIAAYAMMTGRPIDVAFVEEVLANV
                     LRANQRRISIDEIQTQVAEHYRIRKAEMTSARRAREVARPRQVAMYLSKQLTPKSLPD
                     IGRRFGGRDHTTVIHAVKQIERLRAADAELDADIRLLTRQLEG"
BASE COUNT          435 a          912 c          984 g          443 t
ORIGIN      
        1 ggcgggccat ggctcagaac cccgtcgggc cgccggacag cagcggcgcc gccgaatccc
       61 tggccgaaag ccggatttcg ctggccggga ccggccagtc gatcgcgatg tcggggtcgt
      121 cgtagcggat cgagcgctcg agctccgggc gatagggtgc cgagaccttg tactggacct
      181 cggtgtgctc ctcgagggtg acgaagccgt gcgcgaatcc ttccgggacc agcagctggt
      241 tccacagctc ggccgacagg gtcagccccg cccattgccc gaaggtcggc gagcccgggc
      301 ggatgtccac cgcgacgtcg aagatcgacc cgcgcgacac ccgcaccagc ttgtgctggg
      361 cggccggggg cacctggtaa tggaggccgc ggagcactcc ccgcgcagcc gagtaggagt
      421 ggttgtcctg gacgaagccg atctcgaggc cggcctccgc catgttctcg gcgctccaca
      481 cctgcgagaa gaagccgcgc tcgtcgccga acctcggcgg gcggatttcg accagcccgt
      541 cgagcgccag gggccgcgta tcaagcaccg gcgatctcct tggcgcggcg gcggatgtag
      601 tcggcatagc cggtcttgcc gagctggtcg gcgcgggcgc gcacctggtc gggggtcaga
      661 taaccgagct cgagcgcgat ctcctcgagg cacatgacct tgagcccctg gcgatgctcg
      721 atggtgcgga cataggacga ggcgtcgtgc aagctctcgt gggtcccggt gtcgagccag
      781 gcgaagccgc ggccgagccg ggtgacgtgc agcgtcccgc gctcgaggta agcggaattg
      841 acgtcgctga tctccagctc gccgcgcgcc gaaggagtca gtcccgcggc gatgtcgagc
      901 acgtcattgt cgtagaaata gaggcccgtc accgcccagt tggactgcgg ctcggcgggc
      961 ttttcctcga tggtcagcgc ccggccggtc tcgggatcga aggtgaccac tccgtagcgc
     1021 tcggggtcct cgaccccgta ggcgaacacg gttgcgcccc gggtctgcgc ggccgcctgg
     1081 cggaccatct cgacgaggcc cgcgccgtag aagatgttgt cgcccaggac gagcgcgacc
     1141 gggctgtcgc cgacgaactc gcggccgatg atgaatgctt cggcgagccc gttaggctcg
     1201 ggctgctcgg cgtaggagaa ggaaactcca taggcgcttc cgtcgccgag cagctcgcgg
     1261 aaccgcggca ggtcggtcgg ggtcgagatg atcaggattt cggtgatgcc ggccagcatc
     1321 agcaccccga gcgggtaata gatcatcggc ttgtcgaaca ccgggagcag ctgcttggaa
     1381 acagccagcg ttgccggata gagccggctg ccgctgcctc ctgcaagaac gatccctttc
     1441 actcgacgct ccgcttggct ggttcgcgtg cttcaccctg gacagggtca cgccggcgac
     1501 gctctaaaca gcttatcggc gccgccgcaa ccgggaccgg gcggccgtcg cggcgcggcc
     1561 ataggaaagg cccacccgga agcgattccg gatgggcctt tttccctggc catcggggca
     1621 accagggaac tcttggccgc tagccctcga gctggcgggt caaaagccgg atatcggcgt
     1681 cgagctcggc gtcggcggcg cgcagccgct cgatctgctt gaccgcgtgg atcacggtcg
     1741 tgtggtcgcg cccgccgaag cggcggccga tgtcgggcag cgacttgggg gtcagctgct
     1801 tcgacaggta catcgccacc tggcgcgggc gggcgacttc gcgggcccgg cgcgccgacg
     1861 tcatctcggc cttgcggatc cggtaatgct cggcgacctg ggtctggatt tcgtcgatcg
     1921 agatccgccg ctggttggcg cgcagcacgt tggccagcac ctcctcgaca aaggcgacgt
     1981 cgatcggccg gccggtcatc atcgcataag cggcgattcg attgagggcg ccttccagct
     2041 cgcggacgct cgaggtcacc cgccgggcga ggaaatcgac cacgttgcgc ggcatctcga
     2101 ccccgggcag cgctcccaat ttggcctgga tgatgttgaa ccgaagctcg aaatcggccg
     2161 cgttgatgtc ggcgaccaag ccccacgaca gccgcgacag gatccgcggc gcgactccgt
     2221 cgaggtcctg cggggcgcgg tcggacgtga tcaccagccg ccgcccggcg gtgatgatct
     2281 cgttcatggt gtggaagaat tcctcctgcg tggaatcctt gccggcgatg aactggacgt
     2341 cgtcgatcaa cagcaggtcg gcgttgcgca gccgaccctt gaagccgatc gtgtcgttgg
     2401 ccttcaatgc ccggatgaac tcgaccatga acttttcggc cgacatcgag acgacccgct
     2461 tgccggggtt ggcggcgagg aagccgtggc cgatcgcgtg cagcaaatgg gtcttgccgc
     2521 ggccggtgcc gccgtggatg aacagcgggt tgaagccgac cgcgccggtc ccagccagcg
     2581 tccgggcggc ggtcgcggcg acttcattgg ccttgccgac gatgaacgtc tcaaagcggt
     2641 aacgcgggtc gaagttcggg gtcgacgggt cgcgcgcggc cggcggcgcc tccggggcct
     2701 cctcgaggat cagcaacggc gacggccgcg gggcgtcggc cgcggcgccg acccgcactt
     2761 cgcggacgat cggc
//
LOCUS       DASTZO010000023         5453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_471675,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000023 DASTZO010000000
VERSION     DASTZO010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_471675"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFU20_00540"
     CDS             <1..792
                     /locus_tag="VFU20_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU5284978.1"
                     /translation="DDFQPGKLAQNTRRDKLPAVLDFAFKEGALRTIAGTAATELWRD
                     FFSQDVLYEGGEAAAVQLPTFLGNHDMGRFAFYVRKAFPRASEDELLKRVMLGHVLML
                     TARGVPTIYTGDEQGFIGDGGDQDAREDMFPSRTAVYNDNDLLGTRATTAQSNFDPGH
                     PLYRLIGQLSAIRIREPALRRGATVLRSAGDEPGLLAFSRVLDEREVLVAINTSTRPV
                     AQNVPVGIDSDRFTALAGRCPARASAPGSVRITLAPLGYAICNAR"
     gene            782..2395
                     /locus_tag="VFU20_00545"
     CDS             782..2395
                     /locus_tag="VFU20_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEU5284979.1"
                     /translation="MLAEELNARTAARLAPWWRGAAIYQIYPRSFADSNGDGIGDLRG
                     VTAHLDHVAGLGVDAIWLSPFFTSPMRDFGYDVADYCGVDPVFGTLADFDALVERVHS
                     LGLKLIIDQVYSHSSDQHPWFQESRSSRDGPRSDWYVWADARPDGSPPNNWQSVFGGA
                     AWTWDARRGQYYLHNFLREQPDLNVHNPAVQDALLDVARFWLDRGVDGFRIDAINFAM
                     HDPDLRDNPPAPPGGKRTRPFDFQQHVHNQSHPAIAGFLERLRGVIDGYGDRFTLAEV
                     GGDGSLGEMHTFTEGDSRLHSAYGFDFLYADRLTPELVARVAQDWPDRPGVGWPAWAF
                     ENHDAPRALSRWAPPGHADRFARTKMLLLCAMRGSIILFQGEELGLPQVDVPFEQLQD
                     PEAIANWPQTLNRDGVRTPMPWRSDAPNLGFSIGEPWLPAGEEHRPLAVDRQEGDSGS
                     MLAFSRRCLKLRAGTPALRLGSMTIVEAGEQLLRFEREHGGQRLLCSFNLSDRPAPYR
                     PSGASLVTTGEATPEALGAFSAVIEELGR"
     gene            2392..4425
                     /locus_tag="VFU20_00550"
     CDS             2392..4425
                     /locus_tag="VFU20_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HEU5284980.1"
                     /translation="MIVRTILALLALVLAMPAAAADKVAGAASPGGVLSLDVTINGEG
                     RVGYSVGRLGQPVIGESHLGFLLADAPQLLRNFELVSQASRDIDERWEQPWGEWRFMR
                     NRYRELRLAFQEKSKLKRRMNLVFRLYDDGIGFRYELPAQPNLATANITEELTQFRIV
                     EPGEAWWSPAFESNREEYLYNRTPISGIATAQTPLTLRTASGTHVAIHEAALVDYSGM
                     NVRQVENGLLEAVLTYSAAGPKVVRATPFETPWRVLLITPDAPSLYGANALILNLNEP
                     NRLGDVGWVRPMKYVGIWWGMHLDTQSWASGAKHGATTAYAMRMVDFAARHGFPGLLI
                     EGWNKGWDGDWFATGDEFSFTEPYPDFDLARVAAYARRKGVSLIGHHETSGNIAHYES
                     QLEAGLDLYRRLGVPAVKTGYVSDAGGIQALGPDGRIRFEWHEGQVMARHHLKVVTEA
                     AKRRIAVNPHEPIKDTGLRRTYPNWVSREGQRGMEYNAWGEPKNPPDHEVNLVFTRML
                     SGPMDYTPGILSLAGRGNTAIPSTLARQLALYVVLYSPIQMAADLPENYEANPKPFQF
                     IKDVPVDWEDSRMLAGEVGDRAVIARKDRNSADWYLGAIGDEHERRFDVGLGFLAPGR
                     RYRAEIYRDGNRADYRTNPRDIVIEKRIVTGGDRLAMRIAPGGGAAVRFVALK"
     gene            4422..>5453
                     /locus_tag="VFU20_00555"
     CDS             4422..>5453
                     /locus_tag="VFU20_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEU5284981.1"
                     /translation="MSGGPRPDSILILGGGTAGWMAANLFQNRWGALGTRVALIESSE
                     IGIIGVGEGSTPQLKAFFDTIGISEQEWMPRCNATYKAGIGFAGWSDRPGFESYFHPF
                     PTTADERTAHKFFYNTRARRTGRDVWAHPDRFFLNSAIARERRAPVAPPDAPHQASYG
                     YHFDAVLLGRDLKDIATGRGVEHVDAKIASVELGEDGDVAALVAGDGRRFEADFFVDA
                     SGFRALIVEQALGEPRRSFAANLFNDRAVVVPTPLPDEGPGSRTLATAKSAGWIWHIP
                     LTNRIGNGYVYASRYIERDDAAAELRAHLGLAADAEVRHLEMRVGRVDRSWVRNCLAI
                     GLAQGFLEPL"
BASE COUNT          840 a         1948 c         1840 g          825 t
ORIGIN      
        1 gacgacttcc agccgggcaa gctcgcccag aacacccgcc gcgacaagct cccggcggtg
       61 ctcgatttcg cgttcaagga aggcgccttg cggaccatcg ccggcacggc ggcgaccgag
      121 ctgtggcgcg acttcttcag ccaggacgtg ctctatgagg gcggcgaggc ggcggccgtc
      181 cagctgccga ccttccttgg caaccacgac atgggccgct tcgctttcta cgtccgcaag
      241 gcgtttccgc gggcaagcga ggacgagctg ctcaagcgcg tgatgctcgg ccacgtgctg
      301 atgctgaccg cgcgcggcgt tccgaccatc tacaccggcg acgagcaggg ctttatcggc
      361 gacggcggcg accaggacgc ccgcgaggac atgttcccgt cgcgcaccgc ggtctacaac
      421 gacaacgatc tgctcggcac tcgagcgacg accgcgcaga gcaatttcga ccccggccat
      481 ccgctctacc ggctgatcgg gcagctgtcg gcgatccgga tccgcgagcc ggcgctgcgc
      541 cgcggggcga ccgtgctgcg ctcggccggc gacgagcccg ggctgctggc cttctcgcgt
      601 gtcctcgacg agcgcgaggt gctggtggcg atcaacacct cgacccggcc ggttgctcag
      661 aacgtacccg tgggaatcga ttccgaccgc ttcaccgcgc tggccggccg ctgcccggcg
      721 cgggcctcgg cgcccggcag cgttcgcatc accctggcac cgctcggcta tgcgatctgc
      781 aatgctcgct gaggagctga acgcacgcac cgccgccaga ttggcgccat ggtggcgcgg
      841 cgcggcgatc taccagatct atccgcgcag cttcgccgac agcaacggcg acgggatcgg
      901 cgacctgcgg ggagtgaccg cgcacctcga ccatgtcgcc ggcctcgggg tcgacgcgat
      961 ctggctgtcg cccttcttca cctcgccgat gcgtgacttc ggctacgacg tcgccgatta
     1021 ttgcggcgtc gacccggtgt tcgggacgct tgccgatttc gacgctctgg tcgagcgcgt
     1081 gcactcgctc ggcctgaagc tgatcatcga ccaggtctat tcgcacagct ccgaccagca
     1141 cccgtggttc caggagagcc gctcgagcag ggacgggccg cgctccgact ggtatgtgtg
     1201 ggccgacgcc cggcccgacg gctcgccgcc caacaactgg cagtcggtgt tcggcggcgc
     1261 ggcgtggacc tgggacgccc ggcgcggcca atattatctg cacaatttcc tgcgcgagca
     1321 gccggacctc aacgtccata acccggccgt ccaggacgcg ctcctggacg tcgcgcgctt
     1381 ctggctcgac cgcggcgtcg acggcttccg gatcgatgcg atcaacttcg ccatgcacga
     1441 ccccgatttg cgcgataatc cgcccgcgcc gccgggtggg aagcggaccc ggccgttcga
     1501 cttccagcag catgtccaca accagtcgca tccggcgatc gccggcttcc tcgagcggct
     1561 tcggggggtg atcgacggct atggcgaccg cttcacattg gccgaggtcg gcggcgacgg
     1621 gtcgctcggc gagatgcaca ccttcaccga aggcgattcg aggctgcaca gcgcctacgg
     1681 cttcgacttc ctctacgccg atcggctgac gcccgagctc gtcgcccggg tcgcgcagga
     1741 ctggcccgac cggcccgggg tcggctggcc ggcctgggcg ttcgagaacc acgacgcgcc
     1801 gcgggcgctg tcgcgctggg cgccgcccgg ccatgccgac cgcttcgccc ggaccaagat
     1861 gctgctgctg tgcgcgatgc gcgggtcgat catcctgttc cagggcgagg agctcgggct
     1921 gccgcaggtc gacgttccgt tcgagcagct tcaggacccc gaggcgatcg ccaactggcc
     1981 gcagacgctc aaccgcgacg gggtgcggac gccgatgccg tggcgcagcg acgctcccaa
     2041 cctcgggttt tcgatcggcg agccgtggct tccggccggc gaggagcacc ggccattggc
     2101 cgtcgaccgg caggagggcg attcgggctc gatgctggct ttctcccgcc gctgcctgaa
     2161 gctcagggcg ggcacccccg cgctgcgcct cggatcgatg accatcgtcg aggccggcga
     2221 gcagctgctc cgcttcgagc gcgagcacgg cggccagcgg ctgctgtgca gcttcaacct
     2281 gtccgaccgg ccggcgccgt accggccctc gggcgccagc ctggtcacga ccggcgaagc
     2341 gacgcccgag gcgctcggcg ccttttccgc ggtcatcgag gagctgggcc gatgatcgtc
     2401 cggacgattc ttgccctgct ggcgctggtg ctggcgatgc ccgccgcggc ggccgacaag
     2461 gtcgccggcg ccgcctcgcc cggcggggtg ctgagcctcg acgtcaccat caacggcgag
     2521 ggccgggtcg gttactcggt cggccggctc ggccagccgg tgatcggcga aagccacctt
     2581 ggcttcctgc tcgccgacgc gccgcaattg ctgcgcaatt tcgagctggt gtcgcaggcc
     2641 agccgcgaca tcgacgaacg ctgggagcag ccgtggggcg agtggcgctt catgcgcaac
     2701 cgctaccgcg agctgcgcct ggcctttcag gagaagagca agctcaagcg ccggatgaac
     2761 ctcgtcttcc ggctctacga cgacggcatc ggctttcgct acgagcttcc cgcgcagccc
     2821 aacctcgcca ccgccaacat caccgaggaa ctgacccagt tccggatcgt cgagcccggc
     2881 gaggcctggt ggtcgccggc gttcgagtcc aaccgcgagg aatatctcta caaccgcacc
     2941 ccgatcagcg gcatcgccac cgcccagacg ccgctgaccc ttcgcaccgc gagcggcacc
     3001 catgtcgcga tccatgaagc ggcgctggtc gattattcgg ggatgaacgt ccgccaggtc
     3061 gagaacgggc tcctggaggc ggtgctgacc tattcggccg ccggcccgaa agtcgttcga
     3121 gcgacgccgt tcgagacccc gtggcgggtg cttctgatca cgcccgacgc gccgagcctg
     3181 tacggagcca acgcgctgat cctcaacctc aacgagccca accggctcgg cgatgtcggc
     3241 tgggtccggc cgatgaaata tgtcggcatc tggtggggca tgcacctcga cacgcaaagc
     3301 tgggcgtcgg gcgccaagca tggagcgacc accgcttatg caatgaggat ggtcgatttc
     3361 gctgcccggc acggctttcc cgggctgctg atcgagggct ggaacaaggg ctgggacggc
     3421 gactggttcg cgaccggcga cgagttcagc ttcaccgagc cctatccgga cttcgacctc
     3481 gcccgggtcg cggcctatgc ccgccgcaag ggcgtcagcc tgatcggcca ccacgagacc
     3541 tcgggcaaca tcgcccatta cgaaagccag ctcgaggccg ggctcgacct ctaccggcgg
     3601 ctcggagtcc ctgcggtcaa gaccggctat gtgtcggacg cgggcggcat ccaggcgctc
     3661 gggcccgacg gccggattcg tttcgagtgg cacgaggggc aggtgatggc gcggcaccat
     3721 ctcaaggtcg tcaccgaggc cgcgaagcgc cggatcgcgg tcaacccgca cgagccgatc
     3781 aaggacaccg gcctgaggcg cacctacccg aactgggtca gccgcgaggg ccagcgcggc
     3841 atggagtaca acgcctgggg cgagcccaag aacccgcccg accatgaggt gaacctggtc
     3901 ttcacgcgca tgctgtcggg gccgatggac tatacgccgg ggatcctcag cctggccggc
     3961 cgcggcaaca ccgcgatccc gtcgaccctg gccaggcagc tggcgctcta tgtcgtcctc
     4021 tactcgccga tccagatggc cgccgacctg cccgagaatt acgaggccaa tccgaaaccg
     4081 ttccagttca tcaaggacgt gccggtcgac tgggaggaca gccggatgct cgccggcgag
     4141 gtcggcgacc gggcggtgat cgcgcgcaag gaccgcaact cggccgactg gtacctgggc
     4201 gcgatcggcg acgagcacga gcgcaggttc gacgtcggcc tcggcttcct cgcgcccggc
     4261 cgccgctacc gcgccgaaat ctaccgcgac gggaaccgtg ccgactaccg caccaacccg
     4321 cgcgacatcg tcatcgagaa gcggatcgtc accggcggcg accgcctcgc catgcggatc
     4381 gcgcccggcg gcggggccgc ggttcggttc gtggcgctga agtgagcggc ggcccgaggc
     4441 ccgactcgat cctgattctc ggcggcggca ccgccggctg gatggccgcc aacctgttcc
     4501 aaaatcgctg gggggcgctc gggacgcgcg tcgcgctgat cgaatcgagc gaaatcggga
     4561 tcatcggcgt cggcgagggc tcgaccccgc aactgaaggc ctttttcgac acgatcggca
     4621 tctccgagca ggaatggatg ccgcgctgca acgcgaccta caaggccggc atcggctttg
     4681 ccggctggtc cgaccggccg ggcttcgaga gctatttcca tccgttcccg acgaccgccg
     4741 acgagcgcac cgcgcacaaa ttcttctaca acacgcgggc gcgccgcacc ggccgcgacg
     4801 tctgggcgca ccccgaccgc ttcttcctca acagcgccat cgcccgcgag cgccgggcgc
     4861 cggtcgctcc gccggacgcg ccgcaccagg cgagctacgg ctaccatttc gatgcggtcc
     4921 tgctcggccg agatctgaag gacatcgcga ccggccgcgg ggtcgagcat gtcgacgcga
     4981 agatcgcctc ggtcgagctg ggcgaggacg gcgacgtcgc cgcgctggtg gccggcgacg
     5041 gccgccggtt cgaagcggat ttcttcgtcg acgcgagcgg ctttcgggcc ctgatcgtcg
     5101 agcaggcgct gggcgagccg aggcgctcgt tcgccgccaa cctgttcaac gaccgcgcgg
     5161 tcgtcgttcc gacgccgctg ccggacgaag gcccgggaag ccgcactctg gcgaccgcga
     5221 aaagcgccgg ctggatctgg cacatcccgc tgaccaaccg gatcggcaac ggctatgtct
     5281 atgcgtcgcg ctacatcgag cgcgacgatg ccgccgccga gctccgcgcc cacctcgggc
     5341 tcgccgcgga cgccgaggtc cgccacctcg aaatgcgggt cggccgggtc gaccgcagct
     5401 gggtccgcaa ctgcctggcg atcgggctcg cccagggttt cctcgagccg ctg
//
LOCUS       DASTZO010000024         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_602306,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000024 DASTZO010000000
VERSION     DASTZO010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602306"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1772)
                     /locus_tag="VFU20_00560"
     CDS             complement(<1..1772)
                     /locus_tag="VFU20_00560"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEU5284982.1"
                     /translation="MAVALIAAPAAAQTTDPATPPDPTVEAQEQPADPEAGAAQTDDA
                     VQTAAGADQAADEGDAIVVTGLRRSLQSAQNIKRNSEQIVDAIVAEDIGKLPDIAVSD
                     TAARIPGVQVERGGGEAGRVLVRGLDNTFYTTTYNGREIFTAETRSVALQDFPAGGIS
                     AIEAFKTSTANLVEPGLAGLINVRSRRPFDFKDFELAGSVWSGYPNQSRDPKVNAQLL
                     VTDRWDAGQGELGALLNFSYTRLHYQDSIRRHGFFIANLAGGRSPDWPEIQYGEGDRW
                     RPSLNGAVQWRPSEDTELYLEGLWQGYREEVSSRMWAQPLWGGASYSNIVFDEGSNRI
                     ISGTVTNPTACCGGNHTWGFQGATKRKTNTYQFAIGGRQDLGPLRITGDIARTSSRFD
                     LQTESVDFEINRKDYSVNWFTGRPGGSGPTFEIVGLNTEDPSIYNYRGFFEDYLTAKG
                     DDWQVRVDGEYELSGIDFLPKIQAGLRFVDRDASARGGARYWNFAQRGIPISAVPLDY
                     QLFQSAFRGDDNKPFPTTWLAPTFRSVHNNLIELRQFNMNLGGTAFGSNSLDDPAPDP
                     GRTFEINEESLAGYAQLNYDFSVG"
     gene            complement(1942..3246)
                     /locus_tag="VFU20_00565"
     CDS             complement(1942..3246)
                     /locus_tag="VFU20_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5284983.1"
                     /translation="MKQSATALLAVALAGCGGAEDSKGIYLQRFFGECTAVYGSTTDL
                     SKVEGECGIITTMINKFNAENPDSPVSQNVVAWPGYPQLTAQVAARDPPDVVTMHTGV
                     MSDYAARGLIEPIEPYLREAGIDPAGFTDASRRAVTLNGRIYGLPWDTHGGLFHVNLA
                     LFKKAGLMRGGKPLLPRSADELLRHARQFKARTGKPYLIQSLVGDPAYQTRNMYTYLM
                     AQDAVIFPDPDHIRLRTPQARRVAEFFRRINVERLGTLNQDTPAAIAAFMNGEGGIYP
                     TGTWMIGQFEAEANTPGRPLHDSYGVYPYPRLWGRHASFVSGHAWVVPARERSPEQRS
                     AMARFFRFMAQHNYDWARTGHLPAFRSVVDSAQFKALPHRQDIAPLATIGQPLPGYVQ
                     RQAAIEGLVGEEMAAAVNGQKPIERALADAERRVNNLLAQVD"
     gene            complement(3243..>4026)
                     /locus_tag="VFU20_00570"
     CDS             complement(3243..>4026)
                     /locus_tag="VFU20_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5284984.1"
                     /translation="LVGAAIMVAPLAWTLLLSLKANSALVGNSGAALSAPYTLENYQA
                     IVGNYLTMRWLLNSVIVSLGTTAGVLVVSSLAGYGFARLDFPFRRTLFVLVLLGLAIP
                     QQAVILPQHRLFAELGLHNSYPGLILPALAGPFGVFFMTQYFRAIPVELDEAAMLDGA
                     SRLRTFWKVLLPLTLPAQATLGVFTFLGSWNDYWWPLISATRSDMYTLTVGLASSQMN
                     YAQTSGLGFLMAQAVFASIPIFIVYIVFQKQIVRAMAGTAVR"
BASE COUNT          643 a         1319 c         1383 g          681 t
ORIGIN      
        1 tcgccgacgc tgaaatcgta gttcagctgt gcgtagccgg cgagcgattc ctcgttgatc
       61 tcgaacgtcc tgcctgggtc gggagccggg tcgtcgagcg agttcgatcc gaacgccgtg
      121 ccgcccaggt tcatgttgaa ctggcgcagc tcgatcagat tgttgtggac gctccggaac
      181 gtcggcgcca gccaggtggt cgggaacggc ttgttgtcgt cgccgcggaa cgccgactgg
      241 aacaattgat agtcgagcgg caccgccgag atcgggattc cccgctgggc gaagttccag
      301 tagcgggcgc cgccccgcgc ggacgcgtcg cgatcgacga agcgcaggcc ggcctggatt
      361 ttcggcagga aatcgattcc ggacagctcg tattcgccgt cgacgcggac ctgccagtca
      421 tcccccttcg cggtcagata atcctcgaag aagccgcgat aattgtagat cgacggatcc
      481 tcggtgttga ggccgacaat ctcgaacgtc ggccccgagc cgcccggccg cccggtgaac
      541 cagttgaccg aataatcctt ccggttgatc tcgaagtcga cgctttcggt ctggaggtcg
      601 aagcggctgc tggtgcgcgc gatgtcgccg gtgatgcgca gcggcccgag gtcctggcgc
      661 ccgccgatcg cgaactgata ggtgttggtc ttgcgcttgg tggcgccctg gaagccccag
      721 gtgtggttgc cgccgcagca ggcggtcgga ttggtgaccg ttccgctgat gatccggttg
      781 cttccctcgt cgaagacgat gttggaatag ctcgctccgc cccacaaggg ctgcgcccac
      841 atgcggctgc tgacttcctc gcgatagcct tgccacaggc cttccaaata cagttcggtg
      901 tcttccgacg gccgccactg cactgcgcca ttgagcgagg ggcgccagcg gtcgccctcg
      961 ccgtactgga tctcgggcca gtcaggcgaa cgtccaccgg cgaggttggc gatgaagaag
     1021 ccgtggcggc ggatcgaatc ctggtagtgc agccgtgtgt aggagaagtt gagcagcgcc
     1081 ccgagctcgc cctggcccgc atcccagcgg tcggtgacca gcagctgcgc gttgactttc
     1141 gggtcgcggg actggttcgg gtagccgctc cacacggagc cggcgagctc gaaatccttg
     1201 aagtcgaacg ggcggcgcga gcgcacgttg atcagcccgg cgaggcccgg ctcgaccagg
     1261 ttcgcggtcg aggtcttgaa cgcctcgatc gcgctgattc cgccggcggg gaaatcctgc
     1321 agggcgaccg agcgggtctc ggcggtgaag atctcccggc cgttgtaggt cgtcgtgtag
     1381 aaagtattgt cgaggccgcg gaccagcacc cggccggcct cgccgccgcc gcgctcgacc
     1441 tggacgccgg ggattcgcgc cgcggtgtcc gacaccgcga tgtcgggcag cttgccgatg
     1501 tcctcggcga cgatcgcatc gacgatctgc tcgctgttgc gcttgatgtt ctgcgccgac
     1561 tgcaggcttc ggcggaggcc ggtgacgaca atcgcgtcgc cctcatcggc cgcctggtcg
     1621 gcgccagccg cggtttgcac cgcgtcgtcg gtctgcgcgg cgccggcttc gggatcggcc
     1681 ggctgctcct gcgcttcgac cgtcgggtcg ggaggggtcg cggggtccgt cgtctgggcc
     1741 gccgcgggcg cggcgatcag cgcaaccgcc atcgcggatg tcgaccagag attcatggaa
     1801 atatggcgca tcgatcggct ccccccacag cgtcacatag ctgtcacatc tatgccgttc
     1861 gtcacatcgg cttacaagcc ctttggacgc aacgcttttg cgatgcgccg cgagctgtgg
     1921 ccgtaaaggc gcaccacggt ctcagtcgac ctgcgcgagc agattgttga cccgccgctc
     1981 ggcgtccgcg agcgcgcgct cgatcggctt ctggccgttg accgcggccg ccatctcctc
     2041 gccgaccagc ccctcgatcg ccgcctggcg ctggacgtag ccgggcagcg gctggccgat
     2101 ggtcgccagc ggcgcgatgt cctgacggtg cggcagggcc ttgaattgcg cgctgtcgac
     2161 gaccgaccgg aacgcgggca gatgcccggt gcgcgcccag tcgtaattat gctgcgccat
     2221 gaacctgaag aagcgcgcca tcgccgagcg ctgttcgggg cttcgctcgc gcgccgggac
     2281 gacccacgcg tggccgctga cgaacgacgc gtggcgcccc cacagccgcg ggtagggata
     2341 gaccccgtag ctgtcatgca gcgggcggcc gggcgtgttc gcctcggcct cgaactggcc
     2401 gatcatccag gttccggtcg ggtagatgcc gccttcgccg ttcatgaacg ccgcgatcgc
     2461 cgccggagtg tcctggttca gagtccccag ccgctcgacg ttgatccgcc ggaaaaactc
     2521 ggcgacccgc cgcgcctgcg gcgtgcgcag ccggatatgg tcggggtcgg ggaagatgac
     2581 cgcgtcctgc gccatcagat aggtgtacat gttgcgcgtc tggtaggcgg ggtcgccgac
     2641 caggctctgg atcagatagg gcttgccggt gcgcgccttg aactggcgcg cgtgccgcag
     2701 caattcgtcg gccgatcgcg gcagcagcgg cttgccgccg cgcatcagcc cggccttttt
     2761 gaacagcgcg agattgacgt ggaacagccc gccgtgggtg tcccacggaa gcccgtagat
     2821 gcgcccgttc agggtcaccg cgcggcggct cgcgtcggtg aagccggcgg ggtcgatgcc
     2881 cgcctcgcga agatagggct cgatcggctc gatcagcccc ctggccgcat agtcggacat
     2941 caccccggtg tgcatggtga cgacgtcggg cgggtccctg gccgcgacct gcgcggtcag
     3001 ctgcggatag ccgggccagg cgacgacgtt ctggctgacc ggcgagtcgg ggttctcggc
     3061 gttgaacttg ttgatcatcg tggtgatgat cccgcactcg ccctcgacct tcgacaggtc
     3121 ggtggtcgat ccgtagacgg cggtgcactc gccgaaaaag cgctgcaggt agatgccctt
     3181 ggaatcctcg gccccgccgc agccggcgag cgcgaccgcc agcaaagccg ttgccgactg
     3241 cttcaccgga ccgcggtccc ggccatcgcc cggacgatct gcttctggaa gacgatgtag
     3301 acgatgaaga tcgggatcga ggcgaacacc gcctgcgcca tcaggaagcc cagcccgctg
     3361 gtctgcgcgt aattcatctg gctcgacgcg aggccgacgg tcagcgtgta catgtcgctc
     3421 ctggtcgccg agatcaaagg ccaccaataa tcgttccacg agcccaggaa ggtgaacacg
     3481 ccgagcgtcg cctgcgccgg cagggtcagc ggcaggagca ccttccagaa cgtccgcagc
     3541 cgggatgcgc cgtcgagcat cgccgcctcg tcgagctcga ccgggatcgc acggaaatat
     3601 tgggtcatga agaacacccc gaacggcccg gcgagcgccg gcaggatcag cccgggatag
     3661 ctgttgtgca gccccagttc cgcgaacagc cggtgctgcg gaaggatcac cgcctgctgc
     3721 gggatggcga ggccgagcag caccagcacg aacagggtgc ggcgaaacgg gaagtcgagc
     3781 cgagcgaagc cgtagccggc gaggctgctg acgaccagca ctccggcggt cgtcccgagc
     3841 gagacgatga cgctgttcag gagccagcgc atggtcagat aattgccgac gatcgcctgg
     3901 taattctcga gcgtgtaggg cgccgacaaa gcggcgccgc tgttgccgac cagagcgctg
     3961 ttcgccttca gcgacaggag cagcgtccag gccagcggag cgaccatgat cgccgccccg
     4021 accagc
//
LOCUS       DASTZO010000025         4541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_993918,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000025 DASTZO010000000
VERSION     DASTZO010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993918"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1345
                     /locus_tag="VFU20_00575"
     CDS             <1..1345
                     /locus_tag="VFU20_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284985.1"
                     /translation="ARDQRPIERSLASVGERLTVEQQSRDWFDDFAAQRYQTFYAGAP
                     TYNANYDYNYDYDDGYLYQVDRDSDLVTALFPLLGGAFSVGQPLPYGYAGYNVPYAYR
                     DLYYDTPDYSYRYGDGAIYRIDPETQLISAVVALLTGQSFGVGQMLPMGYDVYNVPYA
                     YRDTYYDTYDAWYRYDDGYIYQVDPYSRMITGAYPVSYGGYAVGYPVPAYASYGGYGP
                     YAGYPSYSVPYGYQNLYYGAPGYNYHYANGGIYQVDPKTQLIAALVALVTGTNLGVGQ
                     MLPMGYDVYNVPFAYRSTYFDTDDAWYRYDDGYIYEVDPYTRMIETAIPVSYGGYAVG
                     YPVPAAWPGYDVPDMYDDLYYDAPGYDYRYFDGGIYQVDPKTQLVQAVVALLTGQNLG
                     VGQMLPAGYDAYNVPFAYRDRYYDTADSWYRYNDGTIYQVDPQTRMIQAVIDAVA"
     gene            1471..2052
                     /locus_tag="VFU20_00580"
     CDS             1471..2052
                     /locus_tag="VFU20_00580"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU5284986.1"
                     /translation="MMRNFVWVAILGGAVALAGCSTAPEPRRAAPPVAKAEPAPLPYR
                     WTQGNAPQAHADARAVFGPLALRPGQYRWAAAIPEEGETRIVVDLLTQLFYVYRGDRL
                     VGVATISSGKKGKETPLGFWAVMLKKKQGYSRKYDNAPMPFMQMYDEKGIAFHGGPNP
                     GYPSSHGCVRLPVKFAERLYGLTKLGTKVIIEG"
     gene            2081..2512
                     /locus_tag="VFU20_00585"
     CDS             2081..2512
                     /locus_tag="VFU20_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284987.1"
                     /translation="MDEQFAPRPVAGWYLPAAVASLLFMALGCILYLVHVFADPATMP
                     LDQRAAFEAEPAWVTAAYAVAVWIGAAGAALLVMRRKLAEPLLLVSLAAVLVWLAGLL
                     VVTGLRENMSANDLLVAIVVTAITWTIFWFARHSRQRGWLR"
     gene            2536..3408
                     /gene="panB"
                     /locus_tag="VFU20_00590"
     CDS             2536..3408
                     /gene="panB"
                     /locus_tag="VFU20_00590"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU5284988.1"
                     /translation="MSKVTIDTGTSRATPTPVPGKRMTVPSLRGRKAGGKTDQPIVML
                     TAYTMRMAQLLDPHCDMLLVGDSLGQVIYGLPSTIPVSLEMMCAHGAAVVRGSWHALV
                     AVDMPFGSYEGSPEQAFASASRVMKETGCAAVKLEGGEAMAETIAFLSKRGVPVIGHV
                     GLTPQAVNVLGGYGARGREKAEAKKILADARAVDRAGAFCIVVEGVLEDIAAQVTRSV
                     AAPVIGIGASPQCDGQVLVTEDMLGLFERTPRFVKRYDDLAARIGEAAAAYAAEVRSR
                     SFPTADQTYRPKGG"
     gene            3512..3712
                     /locus_tag="VFU20_00595"
     CDS             3512..3712
                     /locus_tag="VFU20_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284989.1"
                     /translation="MKNRISAAALAGALVVSSAAVAAPRDYSEVGVETPGAWFNCVSP
                     YPGPGGFRTGPCWLDYVGRNAG"
     gene            3872..4066
                     /locus_tag="VFU20_00600"
     CDS             3872..4066
                     /locus_tag="VFU20_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284990.1"
                     /translation="MNKAYAAALLIAAATSSAALAAPKGDSSGSGYDEPGKYFNCVIP
                     FPGPKGFRTGACWLDGATPA"
     gene            4288..>4541
                     /locus_tag="VFU20_00605"
     CDS             4288..>4541
                     /locus_tag="VFU20_00605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5284991.1"
                     /translation="MAVPTDSGETFLREVDENLRRDQLRDMARKYGGWLVAALVLFLA
                     AVGGWLYWQDRQKAAAAEDSEVLAQIYTDIGAGRMASVPKR"
BASE COUNT          748 a         1512 c         1518 g          763 t
ORIGIN      
        1 cgcgcgcgac cagcggccca tcgagcgcag cctcgcctcg gtcggcgagc ggctgaccgt
       61 cgagcagcag agccgcgact ggttcgacga cttcgcggcg cagcgctacc agaccttcta
      121 cgccggcgcg ccgacctaca atgccaatta cgattacaac tacgactatg acgacggcta
      181 cctgtaccag gtcgaccgcg acagcgatct ggtgacggcg ctgttcccgc tgctcggcgg
      241 cgcgttctcg gtcggccagc cgctgcccta cggctatgcc ggctacaacg tgccctatgc
      301 gtaccgggac ctttattacg acacgcccga ttattcgtac cgctacggcg acggggcgat
      361 ctaccggatc gacccggaaa cgcagctgat ctcggcggtc gtcgccctgc tcaccgggca
      421 gagcttcggg gtcgggcaga tgctgccgat gggctacgat gtctacaacg tcccctacgc
      481 gtaccgcgac acctattacg acacctacga cgcctggtac cgctatgacg acggctacat
      541 ctaccaggtc gacccctact cgcggatgat caccggcgcg tacccggtgt cctacggcgg
      601 ttatgcggtc ggctatccgg tgcccgctta tgcaagctac ggcggctacg ggccctatgc
      661 gggctatccg agctactcgg tgccctacgg ctaccagaac ctctattacg gggcgccggg
      721 ctacaattac cattacgcca acggcggcat ctaccaggtc gacccgaaga cgcagctgat
      781 cgcggcgctg gtcgcgctgg tcaccggcac caaccttggc gtcggccaga tgctgccgat
      841 gggctacgac gtgtacaacg tgccttttgc gtaccgcagc acctacttcg acaccgacga
      901 tgcctggtac cgctacgacg acggctacat ctacgaggtc gacccgtaca cgcggatgat
      961 cgagaccgcg atcccggtgt cctacggcgg ctatgcggtc ggctacccgg tgcccgcggc
     1021 ctggccgggc tacgacgtgc cggacatgta cgacgacctc tattacgacg ccccgggcta
     1081 cgattaccgc tatttcgacg gcggcatcta ccaggtcgac ccgaagacgc agctggtcca
     1141 ggcggtggtc gccttgctga ccgggcagaa tctcggcgtt gggcagatgc tgccggcggg
     1201 ctacgacgcg tacaacgtgc cgttcgccta ccgcgaccgc tattacgaca cggccgacag
     1261 ctggtaccgc tacaacgacg gcaccatcta ccaggtcgac ccgcagacgc gaatgatcca
     1321 ggcggtgatc gacgccgtcg cctgagccgg cccggctttc gaaaatccga ggggcgctgc
     1381 gaggcgcccc ttttttcgcc gtctctccgg gcgcttggaa tctccgcccg gccatgcaat
     1441 tcagccgccg gtgacgatag ctcggggaca atgatgcgaa atttcgtttg ggtcgcgatt
     1501 cttggcggcg ctgtggcgct ggccggctgc tcgaccgcgc cggaaccccg gcgcgccgcc
     1561 ccgccggtcg cgaaggccga gccggcgccg ctgccgtacc gctggaccca gggcaatgcg
     1621 ccacaggccc acgccgacgc gcgcgcggtg ttcggtccgc tggcgctccg gcccgggcaa
     1681 tatcgctggg cggcggccat cccggaggaa ggcgagaccc ggatcgtcgt cgatctgctg
     1741 acccaattgt tctacgtcta tcgcggcgac cggctggtcg gagtggccac catctcgagc
     1801 ggcaagaagg gcaaggaaac cccgctcggc ttctgggcgg tgatgctcaa aaagaagcag
     1861 gggtacagcc gcaaatacga caatgcgccg atgccgttca tgcagatgta cgatgaaaag
     1921 ggcatcgcct ttcacggcgg ccccaacccc ggctatccgt cgagccatgg ctgcgtccgg
     1981 ctgccggtca agttcgccga gcggctttac ggactgacca agctcggaac caaggtcatc
     2041 atcgagggct aggagccacc ggaacagggg gaattcgatt atggacgagc aattcgcgcc
     2101 gcggcccgtg gccggctggt atctgcccgc cgcggtcgcc tctttgctgt tcatggcctt
     2161 gggctgcatc ctctacctgg tgcacgtgtt cgccgacccg gcgacgatgc cgctcgacca
     2221 gcgcgcggcg ttcgaggccg agccggcgtg ggtgaccgcc gcctatgcgg tcgcggtgtg
     2281 gatcggcgcg gccggggcgg cgctgctggt gatgcgccgg aagctcgccg agccgctgct
     2341 gctggtgtcg ctggccgcgg tgctggtctg gctcgccggg ctgctggtgg tcaccggcct
     2401 gcgcgagaac atgtcggcca acgacctgct ggtggcgatc gtggtgacgg cgatcacctg
     2461 gacgatcttc tggttcgctc gccattccag gcagcggggg tggttgcgct agctccgctc
     2521 ggctaaacgc cgcccatgtc gaaagtcacc atcgacaccg gcaccagccg ggcgacgccg
     2581 acgccggtgc cgggcaagcg catgaccgtg ccgtcgctgc gcggccgcaa ggccggcggc
     2641 aagaccgacc agccgatcgt catgctgacc gcctacacga tgcggatggc gcagttgctc
     2701 gacccgcatt gcgacatgct gctggtcggc gacagcctcg gccaggtgat ctacgggctg
     2761 ccctcgacca tcccggtgag cctcgagatg atgtgcgcgc acggcgccgc ggtggtgcgg
     2821 ggaagctggc acgccttggt cgcggtcgac atgccgttcg gatcgtacga gggctcgccg
     2881 gaacaggcgt tcgcgagcgc ctcccgggtg atgaaggaaa ccggctgtgc ggcggtcaag
     2941 ctcgagggcg gcgaggcgat ggccgagacg atcgccttcc tgtcaaagcg cggggtgccg
     3001 gtgatcggcc acgtcggcct gaccccgcag gcggtcaacg tgctcggcgg ctatggcgcg
     3061 cgcggccgcg agaaggccga ggcgaagaag atcctcgccg acgcccgggc ggtcgaccgg
     3121 gccggagcgt tctgcatcgt cgtcgaaggc gtgctcgagg acatcgcggc acaggtcacc
     3181 cgaagcgtcg ccgcgccggt gatcggcatc ggcgcctcgc cgcaatgcga cggccaggtg
     3241 ctggtcaccg aggacatgct cggcctgttc gagcgcactc cgcggttcgt caagcgctac
     3301 gacgacctcg ccgcgcggat cggcgaggcc gcggcggcct atgccgccga agtgcgctcg
     3361 cgaagcttcc cgacggccga ccagacctat cgcccgaaag gcggatgagg cgggtttaaa
     3421 accggttttc tgctccggaa tgaacggagg tatatgggca ggctaaccag gatcggcgct
     3481 tgctgcggct ccacaagatc aggagccccc catgaaaaac agaatttcgg cagcggcgct
     3541 cgccggtgca ttggtcgtct cgagcgcagc ggtcgccgcg cccagggact attccgaagt
     3601 cggcgtcgaa acccccggcg cgtggttcaa ctgcgtgagc ccataccccg gcccgggcgg
     3661 cttcaggacc ggcccgtgct ggctcgatta cgtcggccgc aacgccggct gagcccggcc
     3721 gtcgcgacgc ggccgcgggg gatggcgctc accgcgccgc ggctcggtcg cgtcagccat
     3781 gttaactata tgcgttatct taaggattaa acacggagtt aacaacagcc ccctacttct
     3841 gtgcgctcca tttcaaagga gagctggaac catgaacaaa gcgtatgcgg cggcactgtt
     3901 gattgctgcg gccacctcga gcgcggcact cgctgcaccg aagggagatt cgtcggggtc
     3961 gggctacgac gagcccggca agtatttcaa ctgcgtgatc ccttttcccg gcccgaaggg
     4021 gttccggacg ggcgcctgct ggctcgacgg ggcgacaccc gcctagacat ttccttgcca
     4081 gtgacagggc ctggtgacag ggcgtggacc cggcgcggcg cgaccgcgcc gggttctgtt
     4141 tgcaatggct cgggctcgcg ggctcgcttg accctttgcc tttgccgccc gagcggctag
     4201 agccctgatg ccagcttccg ttcatctgcg gtggggaagg aagcttccgg cagcgacatt
     4261 tcctgatgac atgagttgag gcacggcttg gcggttccaa cggacagcgg cgaaacattc
     4321 cttcgcgaag tcgacgagaa tctgcgccgc gaccagctgc gcgacatggc tcgcaaatat
     4381 ggcggctggc tggttgcggc gctggtcctg ttcctcgccg cggtcggcgg ctggctctac
     4441 tggcaggacc ggcagaaagc cgccgcggcc gaggattccg aggtcctggc gcaaatctat
     4501 accgacatcg gcgccggccg gatggcgagc gtgccgaagc g
//
LOCUS       DASTZO010000026         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_419523,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000026 DASTZO010000000
VERSION     DASTZO010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_419523"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2032)
                     /gene="putA"
                     /locus_tag="VFU20_00610"
     CDS             complement(<1..2032)
                     /gene="putA"
                     /locus_tag="VFU20_00610"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF008869.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEU5284992.1"
                     /translation="MTHGVPTQDTQLPGAPDAPAGPAFRAPYAPPDDEIAAALLAAAG
                     RSDAAEARIDRAATRLIDGIRARTGGLGGIEDFLHAYSLSTKEGLALMVLAEALLRVP
                     DAVTADRLIEDKLATGDWSVQDVKSNALLVAASAWTLGITARIIHPGETPDSILDGLI
                     RRLGLPAVRAATRQAMRLLGSHFVLGQTIEEALHRAARHPDRRYSFDMLGEGARTADD
                     AERHFISYSRAIDEIGAGAGEGALPDRPGISVKLSALHPRFEAVSHRRVMKELVPRVL
                     ELARRARDHDLNFTIDAEEADRLELTLDVLAAVLPDRSLRGWDGFGLAVQAYQKRAPA
                     VIDWLAETARACDRQLMIRLVKGAYWDTEVKRGQERGLAGYPVFTRKPMTDLCFTACT
                     RKLLEARPVLYPQFATHNALTVAAVIEDAGGVSGYEFQRLHGMGETLYELLLAEHPNA
                     AVRVYAPVGGHRDLLAYLVRRLLENGANSSFVSVAADPSVPVATLLKRPQSWIGDAQH
                     ARHPKIPLPRDLYEPSRRNSPGVEFGDSAGLGALVASARNAALPKLAVPLVDGIELHG
                     IEREVTSPIDGAAIGRVFEGDEAIAAAAMTAAADGFADWSATAVETRAAALDRASDLI
                     EANRGRLIALLQVEGGKTLDDALAEVREAIDFCRYYAAEARTSLAPQVLPGPT"
     gene            2138..3373
                     /locus_tag="VFU20_00615"
     CDS             2138..3373
                     /locus_tag="VFU20_00615"
                     /inference="COORDINATES: protein motif:HMM:NF020650.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5284993.1"
                     /translation="MQLCEATVRCGITSCSLHDHVLYLCNHSNSRWGQPGADVGIKGV
                     RRRMGPVQFIRLCRPSHLLALAIAFAVPAMAQNSNNAQAPAKAPAAAPTPNNGLPRVR
                     FSLDGRVEGPSAMFLLPLERGYFKAAGLQVTIDEAATPLETITRVASGNYDMGFADIN
                     AMIKHRDQNPATAPKAVFMVYNKPPYSIVTRKSRGITEPKQLAGKKLGAPPTGTTVGE
                     WALFAKLNGIDTSKVTIENIAIPVRAPMLAAGQIDAVLGFSFRVYVDLKDRGVPVDDI
                     VLMMMADYGMKLYGNAIIVNPKFAAEKPDAVKAFLSAYLRGLKETIRRPADAIDLTIK
                     REDVARNAVELERLRMAIRDNILTPEVKADGFGTVDMARLEEAIAQLALTHTFKARPK
                     AEDVFDGAFLPPAADRKVH"
     gene            3407..>3636
                     /locus_tag="VFU20_00620"
     CDS             3407..>3636
                     /locus_tag="VFU20_00620"
                     /inference="COORDINATES: protein motif:HMM:NF013633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HEU5284994.1"
                     /translation="MSTRVRIDRLLVERRLFESRAKAQAAIAAGLVTADGVTVRKPSD
                     EVRADAVIGAEPAHPFVSRGGVKLATALEHFG"
BASE COUNT          602 a         1259 c         1227 g          548 t
ORIGIN      
        1 cggtcggccc cggcagcacc tgcggcgcca gcgaggtgcg ggcctcggcg gcgtagtagc
       61 ggcagaagtc gatcgcctcg cgcacctcgg cgagcgcatc gtcgagcgtc ttgccgcctt
      121 cgacctgcag cagcgcgatc agccggccac ggttcgcctc gatcaggtcg ctggcacggt
      181 cgagagcggc ggcgcgggtt tccaccgccg tggcgctcca gtcggcaaaa ccgtccgccg
      241 ccgccgtcat ggcggccgcc gcgattgcct cgtcgccttc gaacacccgg ccaatggccg
      301 cgccgtcgat gggcgaggtg acctcgcgct cgatgccgtg aagctcgatg ccatcgacca
      361 gcggcacggc aagcttcggc agcgccgcgt tgcgcgcgct cgccaccagc gccccgaggc
      421 ccgcgctgtc gccgaactcg acaccgggcg aattgcgtcg cgagggctcg tagagatcgc
      481 gcggcagcgg gatcttggga tggcgggcgt gctgcgcatc cccgatccag ctctgcgggc
      541 gcttgagcag ggtcgcgacc gggaccgagg gatccgccgc caccgacacg aacgacgagt
      601 tcgccccgtt ctcgagcagg cgccgcacca gataggccag caggtcgcga tggccgccga
      661 ccggcgcata gacccgcacg gcggcattcg gatgctcggc gagcaggagt tcgtagagcg
      721 tctcgcccat gccgtgcagc cgctggaatt cgtagccgct gacgccgccc gcatcctcga
      781 tcacggccgc caccgtcagc gcgttgtgag tggcgaactg cggatagagc acaggccgtg
      841 cctccagcaa cttccgggtg caggccgtga agcaaaggtc ggtcatcggc ttgcgggtga
      901 acaccggata gccggcaagc ccgcgctcct ggccgcgctt cacctcggtg tcccagtagg
      961 cgcccttgac caggcggatc atcagctgcc ggtcgcaggc tcgcgccgtc tcggccagcc
     1021 agtcgatcac cgccggcgcg cgcttctggt aggcttggac ggcgaggccg aagccgtccc
     1081 agcctcgcag cgagcgatca ggcaggactg ctgccagcac gtcgagcgtc agctccagcc
     1141 ggtcggcctc ctcggcgtcg atggtgaagt tgaggtcgtg atcgcgagcc cggcgggcca
     1201 gctccagcac ccgcggcacc agttccttca tcacccggcg gtgcgagacc gcttcgaagc
     1261 gcggatgcag cgcggacagc ttgaccgaaa tgccgggccg gtcgggcaag gcgccctcgc
     1321 cagccccggc gccgatctcg tcgatggcgc gggaatagga gatgaaatgg cgctcggcgt
     1381 cgtccgcggt acgcgcgcct tcgccgagca tgtcgaaaga atagcgccgg tcggggtgcc
     1441 gcgcggcgcg gtgcagcgcc tcttcgatgg tctgcccgag cacgaaatgc gagccgagca
     1501 gccgcatcgc ctggcgagtc gcggcgcgca ccgccggcag gccgagccgc cggatcaggc
     1561 cgtccaggat ggagtcgggc gtttcgcccg gatggatgat gcgggcggtg atgccgagcg
     1621 tccaggccga ggcggcgacc agcaaggcgt tcgacttcac gtcctggacc gaccagtcgc
     1681 cggtcgcgag cttgtcctcg atcaaccggt cggcggtgac ggcgtccgga acccgcagca
     1741 gcgcctcggc cagcaccatg agggcgagcc cctccttggt cgacagcgag taggcatgca
     1801 ggaaatcctc gatgccgccg agcccgccgg tgcgcgcacg aatgccgtcg atcagccggg
     1861 tggcggcacg gtcgatccgc gcctcggcgg cgtccgagcg gccagccgcc gccagcaacg
     1921 cggccgcgat ctcgtcgtcg ggcggcgcgt agggcgcgcg aaacgccggc ccggcgggtg
     1981 catccggggc gccgggtagc tgcgtatctt gggtcggcac accgtgggtc atggagcggt
     2041 cccggccgtg cgtcacggcc ttaatcttaa tggcaacccg atgctgccac cgccacgaaa
     2101 tgatgctcct gtgggatcac aatgccccgc gcggggtttg cagctgtgtg aagccacagt
     2161 tcgatgcggt atcacgtctt gctctttgca tgatcatgtt ctttatctct gcaaccactc
     2221 caattcgcgc tggggccagc cgggagccga cgttggtata aagggagtac ggcgacgcat
     2281 gggccccgtt caattcatcc ggctttgtcg gcccagccac ctgcttgcat tggccatcgc
     2341 cttcgcggtg ccggccatgg cgcagaactc caacaacgcg caggcgcccg ccaaagcgcc
     2401 ggccgcagcg cctaccccga acaacgggct gccgagggtg aggttttcgc tcgacgggcg
     2461 agtggaaggg ccttcggcca tgttcctcct cccgctcgag cgggggtatt tcaaggcggc
     2521 gggcctccag gtgaccatcg acgaagccgc cacgccgctc gagaccatca cccgggttgc
     2581 ctcgggcaac tacgatatgg gctttgccga catcaatgcc atgatcaagc atcgggatca
     2641 gaacccggcg accgccccga aggcggtgtt catggtctac aacaagccgc cctactcgat
     2701 cgtgacccgc aagagccgcg gcatcaccga gcccaagcag cttgccggca agaagctcgg
     2761 ggcgcccccg accggcacca cggtcggcga atgggcgctg ttcgccaagc tcaacggcat
     2821 cgatacgtcg aaggtcacca tcgagaacat cgccatcccg gtgcgcgcac cgatgctcgc
     2881 cgccggccag atcgatgccg tgctcgggtt ctcgttccgg gtctatgtcg acctcaagga
     2941 ccgcggcgta ccggtcgacg acatcgtcct gatgatgatg gcggactacg ggatgaagct
     3001 gtacggcaac gccatcatcg tgaacccgaa gtttgccgcc gagaagccgg acgccgtcaa
     3061 ggcgttcctg agcgcctacc tgcgcggcct gaaagagacc atccgccgcc ccgccgatgc
     3121 gatcgacctg actatcaagc gcgaggacgt cgcccgcaat gcggtcgagc tcgaacgcct
     3181 gcggatggca atccgggaca acatcctcac cccggaagtg aaggccgacg gcttcggcac
     3241 ggtcgacatg gcgcggctcg aagaagccat cgcccaactg gcgttgaccc acacgttcaa
     3301 ggccaggccg aaagccgagg atgtgttcga cggcgcgttc ctgccgccgg ccgccgaccg
     3361 caaggtccat tagacgaatg tcccgatccc gctagcggga aaggtaatgt ccacccgtgt
     3421 ccgcatcgac cgccttctcg tcgaacgtcg cctgttcgag agccgcgcca aggcgcaggc
     3481 cgcaatcgcc gccggcctgg tgacggcgga cggcgtgacg gtcagaaaac cgtccgacga
     3541 agtccgggcc gacgcggtga ttggcgctga gccggcgcac cccttcgtgt cgcggggcgg
     3601 cgtgaagctt gcgaccgcgc tcgaacattt cggctt
//
LOCUS       DASTZO010000027         5576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1020227,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000027 DASTZO010000000
VERSION     DASTZO010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020227"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="VFU20_00625"
     CDS             <1..977
                     /locus_tag="VFU20_00625"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU5284995.1"
                     /translation="RAMCDSMSEALLGWRSDPNIEAVIIDHAEGRGFCAGGDVVMLAR
                     SGAGDGDQARAFFHSEYRLNHLLFTYPKPTVALMDGITMGGGVGISLPCTYRVATENT
                     RLAMPETGIGLFPDVGGGWYLSRLPGRVGQFMVLTGARLDGAECHYLGLATNYVAHSS
                     LEELVERIARMPMRVGGVLGAASATPPDARIAANLGTIARAFASDRLEEIEAALEADE
                     SDWARTELATIRTKSPLSCKVSLRLLAEGAARRSFADEMAAEYALATRVVRTHDFREG
                     VRALLIDKDNEPKWDPPSPDEVSEDMVDALFEPLPEDQRWTPFPAAGG"
     gene            979..1755
                     /locus_tag="VFU20_00630"
     CDS             979..1755
                     /locus_tag="VFU20_00630"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU5284996.1"
                     /translation="MSEYQTIIVERREAVTLVTLNRPQALNALNNQVLSELIAAFAAY
                     DSDPSQRCLVLTGSEKAFAAGADIKEMQGQGFADMYSANHFGGWEQVTATRKPWLAAV
                     AGFALGGGCEVAMMADFIIAADTAKFGQPEIKLGVTPGMGGSQRLALAIGKAKAMEMC
                     LTGRMMDAAEAERAGLVAKVVPAADLIDEAMKTAAQIAAMAPLAAIAVKEMVNAAFEM
                     PLSQGIRFERRLFHGLFGTDDQKEGMTAFVEKRPGNWTGR"
     gene            1769..2677
                     /gene="mmsB"
                     /locus_tag="VFU20_00635"
     CDS             1769..2677
                     /gene="mmsB"
                     /locus_tag="VFU20_00635"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HEU5284997.1"
                     /translation="MAKIAFIGLGHMGGGMAPNLAKAGHEVRAFDLSEEALGRAVEHG
                     CTAAASAAEACKDADAVVTMLPAGQHVAEVYRNSVFGAAPKSAILIDCSTIDIATARS
                     IEEEAAAAGLTMVDAPVSGGIAAAEAGTLTFMVGGSDDGFEKARPFLEKMGKAVIHAG
                     GPGSGQAAKICNNMLLGATMAATCETFVLAQKLGLDLQTFYDIASKASGQSWSMTSYC
                     PVPGVGPETPADRGYEGGFAAALMLKDLKLAMEAAEQAGAYTPMGGEAEELYRRFVDL
                     GGGGKDFSGIIKMIDDSWTPTDGNQG"
     gene            2687..3256
                     /locus_tag="VFU20_00640"
     CDS             2687..3256
                     /locus_tag="VFU20_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5284998.1"
                     /translation="MRTALLAAAAAITLLAGCREAADDQAANTAAAKDRPVFPVTDDD
                     PAAATPAAAAPRNKEEALKLMHERHEGMEDIGDAFKVLSREMKSDSPDLAAVRTHSAT
                     VARLAPQVSRWFPAGTGPDVGKTRAKSEIWQKPEDFAARTRAFQAAAQKFDATAKAGD
                     VAAIKASFGEVGKTCKACHDPYRAPESDH"
     gene            3234..3929
                     /locus_tag="VFU20_00645"
     CDS             3234..3929
                     /locus_tag="VFU20_00645"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEU5284999.1"
                     /translation="MHPNPTTELVEAPPPRPVWDLPIRLFHWLLTALIAFSWWTAKNG
                     QLDWHVWSGVAILTLLVFRLLWGLFGSSTARFANFVRGPRAIRDYLRNVHGWRLAGHN
                     PLGAMSVIVLLLATALQVGLGLISVDEDGLNEGPLAHLVSLEASEAARDLHEDFFNVL
                     LALIVLHVAAILFYRLAFGRRLTAPIITGRAALDPDVEPMRPARAWVALACLAAAIAV
                     GRWVAAGAPPFGR"
     gene            3988..4560
                     /locus_tag="VFU20_00650"
     CDS             3988..4560
                     /locus_tag="VFU20_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEU5285000.1"
                     /translation="MLIRTEQTPNPATRKFLPGETVMEAGNRDFADRESAEASPLASA
                     LFATGLVEGVFFGRDFISVTAAPGVSWDELEPEVVGMLLDHFVSGAPLFNVGTAAGIE
                     VAAPPHIDEDPADADIIAQIRELIETRVRPAVAQDGGDIVYRGYRDGRLYLAMHGACH
                     GCPSSEITLKRGIENLIRHYVPEVETVEAV"
     gene            4635..5264
                     /gene="tsaB"
                     /locus_tag="VFU20_00655"
     CDS             4635..5264
                     /gene="tsaB"
                     /locus_tag="VFU20_00655"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU5285001.1"
                     /translation="MLLAIDTSTAACTAALFDRAGRCVAQRDERIGRGHAERLVPMIE
                     ELLERRSARRILVGVGPGSFTGIRVGIAAAQGLAIGWEAELSGMSSLALLACGAPAGL
                     AVAAAMQGGHGELFVQQFEGQPLQPSTPLLNLPAAEAATRITAELVVGPGAAELVAAR
                     GRGEALDAWPSAADALKLPDELRALPPRPLYARAPDARARPSLAATEAA"
     gene            5264..>5576
                     /locus_tag="VFU20_00660"
     CDS             5264..>5576
                     /locus_tag="VFU20_00660"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5285002.1"
                     /translation="MGSLPSAEAIRLVDGTSTDLSAVMTIMNAAFAARFGEAWTRSQC
                     AGILPMGGVSLVLAHDNGDERPVGFSLFRTVADEAELLLLAVAPEHQRRGIGRLLLEQ
                     FV"
BASE COUNT          841 a         1943 c         1966 g          826 t
ORIGIN      
        1 cccgtgcgat gtgcgattcc atgtccgaag cgctgctcgg ctggcgctcc gacccgaaca
       61 tcgaggcggt gatcatcgat cacgccgaag gccgcggctt ttgcgccggc ggcgacgtgg
      121 tgatgctcgc ccggagcgga gcgggcgacg gcgaccaggc gcgcgccttc ttccacagcg
      181 agtatcggct caaccacctt ctgttcacct accccaagcc gacggtcgcc ctgatggacg
      241 ggatcaccat gggcggcggg gtcggcatct cgctgccgtg cacctaccgg gtcgcgaccg
      301 agaacacccg gctggcgatg ccggaaacgg gcatcggcct gttccccgac gtcggcggcg
      361 gctggtacct gtcgcgcctg ccgggccggg tcgggcagtt catggtcctg accggcgccc
      421 ggctcgacgg cgccgagtgc cattatctcg gcctggcgac caattacgtg gcgcactcgt
      481 cgctcgagga gctggtcgag cggatcgcca ggatgccgat gcgggtcggc ggcgtcctcg
      541 gggccgcctc ggcaacgccg cccgacgcca ggatcgccgc caacctcggg accatcgcgc
      601 gcgcctttgc ctcggaccgg ctcgaggaga tcgaggcggc gctcgaggcc gacgaaagcg
      661 attgggcgcg gaccgagctg gcgaccatcc gaaccaagag cccgctgtcg tgcaaggtct
      721 cgctcaggct gctggccgaa ggcgcggcgc gccgcagctt cgccgacgag atggcggccg
      781 aatatgcgct cgcgacgcgg gtcgtccgga cccacgactt ccgcgaaggc gtgcgcgcgc
      841 tgctgatcga caaggacaat gagcccaagt gggacccgcc gtcgccggac gaggtcagcg
      901 aggacatggt cgacgccctg ttcgagccgc ttcccgagga ccagcgctgg acgccgttcc
      961 ccgcggccgg aggatgagat gagtgagtat cagacgatca tcgtcgagcg gcgcgaggcg
     1021 gtgactctcg tcaccctcaa ccggccgcag gcgctgaacg cgctcaacaa ccaggtgctg
     1081 agcgagctca tcgccgcatt tgccgcctat gattcggatc cctcgcagcg ctgcctggtc
     1141 ctcaccggat ccgaaaaagc cttcgccgcc ggcgccgaca tcaaggaaat gcagggccag
     1201 ggctttgccg atatgtactc ggccaatcat ttcggcggct gggagcaggt caccgcgacc
     1261 cgcaagccgt ggctggccgc ggtcgccggc ttcgcattgg gcggcggctg cgaggtggcg
     1321 atgatggccg atttcatcat cgccgccgac accgccaagt tcggccagcc ggagatcaag
     1381 ctcggggtca ctcccgggat gggcgggtcg cagcgcctgg cgctggcgat cggcaaggcc
     1441 aaggcgatgg agatgtgcct gaccgggcgg atgatggacg ccgccgaggc cgagcgcgcc
     1501 ggcctggtcg ccaaggtcgt cccggcggcg gaccttatcg acgaggcgat gaaaacggcg
     1561 gcgcagatcg cggcgatggc gccgctggcc gcgatcgccg tcaaggagat ggtcaacgcc
     1621 gcgttcgaga tgccgctttc gcagggcatc cggttcgagc ggcggctgtt ccacgggctg
     1681 ttcgggaccg acgaccagaa ggaggggatg accgccttcg tcgagaagcg ccccggcaac
     1741 tggaccgggc gctgagagtt agcgggacat ggcaaagatc gcattcatcg gccttggcca
     1801 catgggcggc gggatggccc ccaacctcgc caaggcgggc cacgaagtgc gcgcgttcga
     1861 cctcagcgaa gaggcgctgg gccgggcggt cgagcacggc tgcaccgccg cggcgtccgc
     1921 ggccgaagcc tgcaaggatg ccgatgcggt ggtgaccatg cttccggccg gccaacacgt
     1981 cgccgaagtg tatcggaatt cggtgttcgg ggcggcgccg aaaagcgcga tcctgatcga
     2041 ttgctcgacg atcgacatcg cgaccgcccg ctcgatcgag gaggaagcgg cggccgccgg
     2101 gctgacgatg gtcgacgcgc cggtgtcggg cgggatcgcg gccgccgagg ccggcaccct
     2161 gacctttatg gtcggcggca gcgacgacgg gttcgaaaag gccaggccgt tcctcgagaa
     2221 gatgggcaag gcggtgatcc atgccggcgg cccgggctcg ggccaggcgg ccaagatctg
     2281 caacaacatg ctactgggcg cgaccatggc cgcgacctgc gagacgttcg tgctggcgca
     2341 aaagctcggg ctcgacctgc agaccttcta cgacatcgcg tccaaggcgt cgggccagag
     2401 ctggtcgatg acgtcctact gcccggtgcc cggggtcggg cccgaaaccc ccgccgaccg
     2461 cggctatgaa ggcggcttcg cggccgcgct gatgctcaag gatttgaagc tggcgatgga
     2521 agcggccgag caggccgggg cctacacgcc gatgggcggc gaggccgagg aactgtaccg
     2581 gcgatttgtc gatttgggcg gtggcgggaa ggacttttcc gggatcatca agatgatcga
     2641 cgacagctgg actccgaccg acgggaatca aggctaagga gagacgatgc gcactgccct
     2701 gctcgccgcc gctgcggcca tcacgttgct ggccggctgc cgcgaggccg ccgacgacca
     2761 ggcggccaac actgccgcgg ccaaggaccg gccggttttc ccggtgaccg acgacgatcc
     2821 cgccgccgcg acccccgcgg ccgccgctcc gcgcaacaag gaagaagcgc tcaagctgat
     2881 gcacgagcgc cacgagggaa tggaggacat cggcgacgcg ttcaaggtgc tcagccgcga
     2941 gatgaagtcg gactcgccgg acctcgcggc cgtccggacg cactcggcga ccgtcgcgcg
     3001 gctcgctccc caggtttccc gctggttccc ggccggcacc ggccccgacg tcggcaagac
     3061 ccgcgccaag tccgaaatct ggcagaagcc ggaggatttc gccgccagga cccgggcgtt
     3121 ccaggccgcg gcgcaaaagt tcgacgcgac cgccaaggcc ggcgacgtcg ccgcgatcaa
     3181 ggccagcttc ggcgaggtcg gcaagacgtg caaggcgtgc cacgacccct atcgtgcacc
     3241 cgaatccgac cactgagttg gtcgaagcgc cgccgccgcg gccggtctgg gacctgccga
     3301 tccggctgtt ccactggctt ttgacggcgc tgatcgcgtt cagctggtgg acggccaaaa
     3361 atgggcagct cgactggcat gtctggtcgg gcgtcgcgat cctcaccctg ctggtgttcc
     3421 gattgctgtg gggcctgttc ggaagctcga ccgcgcgctt cgccaatttc gtccgcggcc
     3481 cgcgggcaat tcgggactat ctgcgcaacg tccacggctg gcgcctggcc gggcacaacc
     3541 cgctcggcgc catgagcgtg atcgtcctgc tgctcgcgac cgcgctccag gtcgggctcg
     3601 gcctgatctc ggtcgacgag gacggcctca acgaaggccc gctcgcgcac ctcgtcagcc
     3661 tcgaggcttc cgaagccgcg cgcgacctgc acgaggattt cttcaatgtg ctgctcgcct
     3721 tgatcgtgct gcacgtcgcg gcgatccttt tctaccggct ggcgttcggc cgaaggctca
     3781 ctgcgccgat aatcaccggc cgggccgcgc tcgaccccga tgtcgagccg atgcgcccgg
     3841 cgcgcgcctg ggtcgcgctc gcctgcctgg ccgccgctat cgccgtcggc cggtgggtcg
     3901 ccgccggggc gcccccgttc ggccgctgac cgccgccatc ggatcgccgc ccttgatcgg
     3961 gagggaccgc agggccacat ccccctcatg ctgatccgca ccgagcagac gcccaatccc
     4021 gcgacccgca agttccttcc gggcgagacg gtgatggagg cgggcaaccg cgatttcgcc
     4081 gaccgcgaat cggcggaggc ctcgccgctg gcgtcggccc tgttcgccac cggcctggtc
     4141 gaaggcgtgt tcttcggccg cgacttcatc tcggtgaccg cggcgccggg cgtgtcgtgg
     4201 gacgagctcg agcccgaggt ggtcggcatg ctgctcgacc atttcgtcag cggcgcgcct
     4261 ctgttcaacg tcggcaccgc cgccgggatc gaggtcgccg cgccgccgca catcgacgag
     4321 gacccggccg acgccgacat catcgcccag atccgcgagc tgatcgaaac ccgggtccgg
     4381 ccggcggtcg cccaggacgg cggcgacatc gtctatcgcg gctatcgcga cggccggctg
     4441 tacctggcga tgcacggcgc ctgccacggc tgcccgtcgt ccgaaatcac cctcaagcgc
     4501 gggatcgaga acctcatccg ccactatgtg ccggaggtcg agacggtcga ggccgtctga
     4561 ctcaaaacac cttttcgctt gctcgcgcac cgagcctgtg gcccactcgc cgacgcgcaa
     4621 ccaaggcgat cccgatgctg cttgcgatcg acacctcgac ggctgcctgc accgcggccc
     4681 tgttcgaccg ggcgggccgc tgcgttgccc agcgcgacga gcggatcggc cgcggccatg
     4741 ccgagcggct ggtgccgatg atcgaggagc ttctcgagag gcgcagcgcg cggcgcatcc
     4801 tggtcggggt cgggccgggc agcttcaccg gaatccgcgt cgggatcgcc gccgcccagg
     4861 gcctcgcgat cggctgggag gccgagcttt ccggcatgtc gtcgctggcg ctgctcgcct
     4921 gcggcgcacc ggccgggctt gcggtcgcgg cggcgatgca gggcggccac ggcgagctgt
     4981 tcgtccagca gttcgagggc cagccgttgc agccctcgac cccgctgctc aacctgcccg
     5041 cggccgaggc ggcgacccgg atcacggccg agctggtggt cggtccgggc gcggccgagc
     5101 tcgtcgcggc gcgcggccgg ggcgaggcgc tcgacgcctg gccgagcgcg gcggatgctc
     5161 tgaagcttcc ggacgagctg cgggccctgc ccccgcggcc gctctacgcg cgggctccgg
     5221 atgcccgcgc gcgcccatcc ctcgcggcga ccgaggctgc gtgatgggca gcctcccgtc
     5281 cgccgaagcg atccgtctgg tcgacggcac atcgaccgac ctttccgccg tcatgacgat
     5341 catgaacgcg gcgttcgcgg ctcggttcgg cgaagcctgg acccgctcgc aatgcgccgg
     5401 gatcctgccg atgggcggcg tgtcgctggt gctcgcccac gacaatggag acgagcgccc
     5461 ggtcggcttt tcgctgttcc ggacggtggc cgacgaagcc gagctgctgc tgctcgcggt
     5521 cgcgcccgaa caccaaaggc gcggcatcgg ccgattgctg ctcgagcagt tcgtcg
//
LOCUS       DASTZO010000028         2069 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_393199,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000028 DASTZO010000000
VERSION     DASTZO010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2069
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393199"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VFU20_00665"
     CDS             complement(<1..1074)
                     /locus_tag="VFU20_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5285003.1"
                     /translation="MSDLFSKFDPLIEQRRALLDTGLTDPFNLVMERVESPTVAICNG
                     KRTILLGTYNYMGMTFDPDVIAAGKTALEEFGSGTTGSRVLNGTYSGHRAVEQALRDF
                     YAMDHAMVFSTGYQANLGVISTLAGKDDYIVLDIDSHASIYDGCALGNAQIVPFRHND
                     VEALEKRLKRIPAEAGKLVVLEGVYSMLGDVAPLTEMVRVAKENGAMVLVDEAHSMGF
                     IGDHGRGVAEAQGVIDDVDFIIGTFSKSVGTVGGFCVSNHPKFEILRLVCRPYVFTAS
                     LPPSVVATAATSIRKLMDAAPKRAHLWENSKRLHAGLRQLGFRLGTDEPQSAIIAVLM
                     PDLETGAAMWEALLERGLYVNLAR"
     gene            complement(1071..1334)
                     /locus_tag="VFU20_00670"
     CDS             complement(1071..1334)
                     /locus_tag="VFU20_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5285004.1"
                     /translation="MTDRDETAARIQALIEPFNKKGIAVTEATRFAQDLEWDSLTVMD
                     FVASVEDEFDILISMNRAAEIETVGQLVDAVGQLQGDQLQGGR"
     gene            complement(1409..>2069)
                     /locus_tag="VFU20_00675"
     CDS             complement(1409..>2069)
                     /locus_tag="VFU20_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU5285005.1"
                     /translation="NVDGTRSMLDAAKAAGVRRFVHVSSIAAREPDLSLYGGSKARSE
                     ELVAGSGLPFAIVRPPAVYGPGDKETLELFRMARLGVVLLPPEGRLSLIHADDLARLL
                     VALAAPDAPSGVVIEPDDGRAGGWSHKEFAQALGAAVGRKGASLSIPPGLLRLGAMAD
                     RLLRGDRAKLTADRAAYFCHPDWVVDPARAAQPGLWQPRIATPDGLAATAQWYRAQGW
                     L"
BASE COUNT          316 a          711 c          726 g          316 t
ORIGIN      
        1 ccgggcgaga ttgacgtaga ggccgcgctc gaggagcgct tcccacatcg ccgccccggt
       61 ctcgaggtcg ggcatcagca cggcgataat cgccgattgc ggctcgtcgg tgccgaggcg
      121 gaagcccaat tgcctcaggc cggcgtgcag ccgcttgctg ttctcccaca gatgggcgcg
      181 cttgggggcc gcgtccatca gcttgcggat cgaggtcgcc gcggtcgcca ccaccgaggg
      241 cggcagcgag gcggtgaaca catagggccg gcagaccagg cgcaggatct cgaacttcgg
      301 gtggttggac acgcagaatc cgccgaccgt gccgaccgac ttggagaagg tgccgatgat
      361 gaagtcgacg tcgtcgatca ccccctgggc ctcggcgacc ccgcggccat gatcgccgat
      421 gaagcccatc gagtgcgcct cgtcgaccag caccatcgct ccgttttcct tcgccacgcg
      481 caccatctcg gtcagcggag cgacgtcgcc gagcatcgaa tagacgcctt cgaggaccac
      541 aagcttgccg gcttcggcgg gaatccgctt aaggcgcttc tccagcgcct cgacgtcgtt
      601 gtggcggaac gggacgatct gggcgttgcc gagcgcgcag ccgtcgtaga tcgacgcgtg
      661 gctgtcgatg tcgaggacga tgtaatcgtc cttgccggcc agggtcgaaa tcaccccgag
      721 gttggcctgg tacccggtcg agaagaccat cgcatggtcc atcgcgtaga agtcgcgaag
      781 cgcctgctcg accgcccggt ggcccgagta ggtgccgttc aggacccggc tgccggtggt
      841 ccccgagccg aactcttcga gcgctgtctt gccggccgcg atgacgtcgg ggtcgaaggt
      901 catgcccatg taattgtagg tgccgagcag gatcgtccgc ttgccgttgc agatcgccac
      961 ggtcgggctc tcgacccgct ccatcaccag gttgaacggg tcggtgaggc cggtgtcgag
     1021 cagggcccgg cgctgctcga tcagcgggtc gaatttcgag aagaggtcgc tcaccgcccg
     1081 ccctgaagct ggtcgccctg gagctggccg accgcgtcga cgagctgccc gacggtctcg
     1141 atctcggcgg cgcggttcat gctgatcaat atgtcgaact cgtcctcgac cgaggcgacg
     1201 aagtccatca ccgtcaaact gtcccactcg aggtcctggg cgaagcgcgt ggcctcggtc
     1261 accgcgatgc ccttcttgtt gaagggctcg atcagcgcct ggatcctggc ggcggtctcg
     1321 tcgcggtcgg tcattgggcg gctcgtagtg gcgggccgcc gctttcgcaa cgcaatgggg
     1381 cgcaaaaacg gcggctcgga gccaaccgct agagccagcc ctgggcgcga taccactgag
     1441 cggtggcggc gaggccgtcg ggggtcgcga tccgcggctg ccacaggccg ggctgggcgg
     1501 cgcgggcggg atcgacgacc cagtcgggat ggcagaagta ggcggcccgg tcggcggtca
     1561 gcttggcccg gtcgccgcgc agcagccggt cggccatcgc cccgagccgc agcaggcccg
     1621 gcgggatcga caggctcgcg cccttgcgcc cgacggccgc tccgagcgcc tgcgcgaact
     1681 ccttgtggct ccagccgccg gcccggccgt cgtcgggctc gatgacgact ccgctcggcg
     1741 catcgggcgc ggcgagcgcg accagcagcc gcgccaggtc gtcggcgtgg atcagcgaca
     1801 gccgcccctc gggcggcagc agcaccaccc cgagcctcgc catccggaac agctcgagcg
     1861 tctccttgtc gccgggccca tagaccgcgg gcggccggac gatcgcgaac ggcaggcccg
     1921 agccggcgac cagctcctcc gaccgcgcct tggagccgcc gtacagcgac aggtcgggct
     1981 cgcgagcggc gatcgacgag acatggacga accgccggac gcctgccgcc ttcgccgcgt
     2041 cgagcatcga gcgggtgccg tcgacgttg
//
LOCUS       DASTZO010000029         5363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_942250,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000029 DASTZO010000000
VERSION     DASTZO010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5363
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_942250"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="VFU20_00680"
     CDS             <1..416
                     /locus_tag="VFU20_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285006.1"
                     /translation="CDETGDWVVSNAGFDVVAVRRDAGPARQVVLPYFSVAVQGGTQI
                     VAKRVGHVALNFPAGSQRAQTRGQAVVRVSRAAASLPANVREILTRRRKPGEPEAAVD
                     PLSDPAVRAAVARATFEHLVGFQMSQDMLRYNATR"
     gene            complement(413..805)
                     /locus_tag="VFU20_00685"
     CDS             complement(413..805)
                     /locus_tag="VFU20_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285007.1"
                     /translation="MVRTLAGLAAGLAVAILSIAAVEAVGNQLYPPPAGYDMTTASAE
                     TLPVETLVWPVIGWFLGSIAGSWVARRASGERWPGWAIAACVLAATVLNLALIRHPLW
                     MIVAGVVAPVAGGWFAQLLPDRKRGAAR"
     regulatory      908..1008
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     regulatory      1013..1113
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            1309..2409
                     /gene="gcvT"
                     /locus_tag="VFU20_00690"
     CDS             1309..2409
                     /gene="gcvT"
                     /locus_tag="VFU20_00690"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEU5285008.1"
                     /translation="MQTLPLDGWHRSRGARMVPFAGYQMPVQYEGIIAEHLWTRENAG
                     LFDVSHMGQLLVHGRDVDGALETLLPGELRMLGDMKLRYTLLLDDHGGIIDDLMATRR
                     GPDFYLVVNGATKHGDIAVLERLLPRGVVVDHMKEQALLALQGPRAAEALEKLAPGVG
                     ELSFMEGGAFRIAGHPAWISRSGYTGEDGFEISVPAAAAAAVADALLADDAVRPVGLG
                     ARDSLRLEAGLPLYGHDLDRDTTPVMAGLTFAIGKRRRAEGGFPGAMRILAEIENGPP
                     QRRVGFDVDGRQPVREGALVLDSEGNEVGRITSGGFSPSLQRPIAMGYVASHLAEPGT
                     GLKLEQRGKLFDARVAAMPFVPHRYHRKGASA"
     gene            2406..2777
                     /gene="gcvH"
                     /locus_tag="VFU20_00695"
     CDS             2406..2777
                     /gene="gcvH"
                     /locus_tag="VFU20_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEU5285009.1"
                     /translation="MSLYFTREHEWIRLDGDVATVGISDHAQQALGDIVFAEVPEAGR
                     KLAKGAEAAVVESVKAASDVYAPVAGEVVEGNAAVADDPALINRDPEGEGWFFKLKLD
                     DPGEVEGLMDEASYRDWVKTL"
     gene            2840..3523
                     /locus_tag="VFU20_00700"
     CDS             2840..3523
                     /locus_tag="VFU20_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5285010.1"
                     /translation="MPELGMAALDLLDPRAHERILDVGCGDGALTRRMVERGAGVVGI
                     DSSPELVAAAFAAGIDARRMDAADMAFAAEFDAAFSNAALHWMLDKERVAAAVFRALK
                     PGGRFAGEMGGEGNLKALREALDSELVIRGYAPPSESSNWYPSPQEFAAVYEAAGFER
                     IDARLIERPTPLAHGIAEWVTTFRKGWLDRAAVPEAERAEIGAAVAASLATDTADYVR
                     LRFIMRKPA"
     gene            3523..4881
                     /gene="gcvPA"
                     /locus_tag="VFU20_00705"
     CDS             3523..4881
                     /gene="gcvPA"
                     /locus_tag="VFU20_00705"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HEU5285011.1"
                     /translation="MRYLPLSDADRSAMLEVIGAGSVDELFRDVPEAARLKDGIAGLP
                     DHASELSVERQLTAMARKNLVAGEAPFFLGCGAYRHHIPASVDHLIQRGEFLTSYTPD
                     QPEIAQGTLQVLFEFQTQVARLYGCEVANASMYDGSTACWEAIGMARRITRRTKTVIS
                     SGLHPHYVSVANTMARFTGDVLDTALPELAAETDSERQIASIDGETSAVLVQYPDILG
                     RVADLAPIAEAAHAAGALLIAVVTEPVALGLIKSPGEMGADIVVGEGQSIGVGLQVGG
                     PYVGLFACREKHVRQMPGRLAGETVDAEGRRGFVLTLSTREQHIRREKATSNICTNSG
                     LCALAFSVHLSLLGEKGLRRLAEINHARAVEVAARLAAIPGVSLVNDSFFNEFTLRLP
                     VEARPAVRELADRGILGGVSLGRLYPGAQALENGLVVAVTETVTDEDIAAFEAGLKEV
                     VR"
     gene            4878..>5363
                     /locus_tag="VFU20_00710"
     CDS             4878..>5363
                     /locus_tag="VFU20_00710"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980038.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HEU5285012.1"
                     /translation="MTVNPSGWRPAAPVKAAPADQQGEGSTVTGNRALMLEEPLLFEI
                     GDTETTGVDFPDVPAATTRLGGLERTGPIGLAGLSEPEAVRHYTRLSRQNYAIDLGLF
                     PLGSCTMKHNPRLNEKMARLPGFADIHPLQPESTVQGALELMDTLAHWLKTLTGMPAV
                     AL"
BASE COUNT          820 a         1872 c         1938 g          733 t
ORIGIN      
        1 attgcgacga gactggcgac tgggtcgtgt ccaacgccgg cttcgacgtg gtcgcggtcc
       61 gccgcgatgc cggccccgcg cggcaggtgg tgctgccgta cttcagcgtc gcggtccagg
      121 gcggcaccca gatcgtcgcc aagcgcgtcg gccatgtcgc gctgaacttc cccgccggaa
      181 gccagcgcgc ccagacccgc ggccaggcgg tggtgcgggt gtcgcgggcc gcggcgagcc
      241 tgccggccaa cgtccgcgag atcctgaccc ggcggcgcaa gcccggggag cccgaagcgg
      301 cggtggaccc gctcagcgat cccgcggtgc gcgcggcggt ggcgcgcgcg accttcgagc
      361 atctggtcgg attccagatg agccaggaca tgctgcgcta caacgcgacc cgctagcggg
      421 cggcgccccg cttccggtcc ggcagaagct gggcaaacca gccgccggcg accggcgcga
      481 ccaccccggc gacgatcatc cacagcggat gccggatcag agcgaggttg aggaccgtcg
      541 cggccagcac gcaggcggcg atcgcccagc cgggccagcg ctcgcccgac gctcggcggg
      601 cgacccagct gccggcgatc gagccgagga accagccgat caccggccag acgagggtct
      661 cgaccggcag cgtctccgcg ctggccgtgg tcatgtcgta gccggccggc ggcgggtaga
      721 gctggttgcc gaccgcttcc accgcggcga tggacaggat tgccacggcc agtccggcgg
      781 ccagcccggc gagggttcga accatcgttg cgctcccatc ccttttgcaa cgcgccgcac
      841 ggcgcggaat tgactcaatg catcggcggg gccaaggctc cgcgccgtct ccggcaagca
      901 attgccggaa ggccgcgcgg gagagctcgc cttgcccagc cgggcatggc ggcgccgaag
      961 gagcaaccgc ccccggaatc tctcaggcca aaggaccgcg cgcgcctgac agtctggaaa
     1021 gcgttccggc tctcaaagcc ggggccgccg aaggggtaac ctgccgtcgc caaagggcga
     1081 ttgcggggaa agctctcagg tcgcagcgac agaagggggc gccggtcagc ggctggggcg
     1141 caagcggccc tcgagcgttg gctgtcgtca cctgtcgcgg gagcgcccga tgagccgaga
     1201 agtccagtga gccgcgaagt ccacgggggc ccgaccgacg accgccaggc gcgcggcgag
     1261 ccctatcgcg gcgacccgga cggcggcgag ggcgctccga ccgaggaaat ccagaccctg
     1321 ccgctcgacg gctggcaccg ctcgagaggg gcccggatgg tcccgttcgc cggctaccag
     1381 atgccggtcc agtatgaggg catcatcgcc gagcatctgt ggactcgcga gaatgccggc
     1441 ctgttcgacg tcagccacat gggccagctg ctggtccacg gccgcgacgt cgacggagcg
     1501 ctcgagacgc tgctgcccgg cgaactcagg atgctcggcg acatgaagct gcgctacacg
     1561 ctgctgctcg acgatcatgg cgggatcatc gacgacctga tggcgacccg ccgcgggccg
     1621 gatttctacc tggtcgtcaa cggcgccacc aagcatggcg acatcgccgt gcttgagagg
     1681 ctgctgccgc gcggggtggt cgtcgatcat atgaaggagc aggcgctgct cgcgctgcag
     1741 ggcccgcggg cggccgaggc cctggagaaa ctggcgccgg gtgtcggcga attgagcttc
     1801 atggagggcg gcgcgttccg gatcgccggc catccggcgt ggatcagccg ctcgggctat
     1861 accggcgagg acgggttcga aatttcggtt ccagccgccg ccgccgcggc ggtcgccgac
     1921 gccctgctcg ccgacgatgc ggtccggccg gtcggcctgg gagcgcgcga ttcgctgcgg
     1981 ctcgaggccg ggctgccgct ctacggccac gacctcgacc gcgacacgac cccagtgatg
     2041 gccgggctca ccttcgcgat cggcaagcgc cgccgcgccg agggcggctt tccgggcgcg
     2101 atgcggatcc tcgccgagat cgagaacggc ccgccgcagc gccgggtcgg cttcgacgtc
     2161 gacggccgcc agccggtgcg cgagggcgcg ctggtgctcg acagcgaggg caatgaggtc
     2221 ggtcgcatca ccagcggcgg cttctcgccc tcgctgcagc ggccgatcgc gatgggctat
     2281 gtcgccagcc atctcgccga gcccggcacc ggactgaagc tcgagcagcg cggcaagctg
     2341 ttcgacgcgc gggtcgccgc gatgccgttc gtaccccacc gctaccaccg caagggagcc
     2401 agtgcatgag cctctatttc acccgcgagc acgaatggat ccgcctcgac ggcgacgtcg
     2461 ccacggtcgg catttccgac catgcccagc aggcgttggg cgatatcgtc ttcgccgagg
     2521 tcccggaagc cgggcggaag ctggccaagg gcgcggaagc ggcggtggtc gaatcggtca
     2581 aggcggcgtc ggacgtctat gcgccggtcg ccggcgaggt ggtcgagggc aatgccgcgg
     2641 tcgccgacga cccggcgctg atcaaccgcg atcccgaggg cgagggctgg ttcttcaaat
     2701 tgaagctcga cgatccgggc gaggtcgaag ggctgatgga cgaagcttcg taccgcgact
     2761 gggtgaagac gctctgagcc gggccgccgc ctcgaccagc cgatgggacg ccgcggacta
     2821 tgcgcgcgtc ggaagcttcg tgcccgagct cgggatggcg gcgctcgacc tgctcgaccc
     2881 gcgggcgcac gagcgcatcc tcgacgtcgg ctgcggcgac ggggcgctga cccgcaggat
     2941 ggtcgagcgc ggagcgggcg tggtcggcat cgacagctcg cccgagctgg tcgcggcggc
     3001 gttcgcggcg gggatcgacg cccggcggat ggacgccgcg gacatggcct tcgctgccga
     3061 gttcgatgcg gccttctcca acgccgcgct gcactggatg ctcgacaagg agcgcgtcgc
     3121 ggccgcggtc ttccgggcgc tgaagcccgg cggccgcttc gccggcgaga tggggggcga
     3181 aggcaacctc aaggcgttgc gcgaggcgct cgattccgag cttgtgatcc gcggctatgc
     3241 gccgccgagc gagagctcca actggtaccc gtcgccgcaa gaattcgcag ccgtctacga
     3301 agcggccggt ttcgagcgaa tcgacgcccg cctgatcgag cggccgacgc cgctcgccca
     3361 cggcatcgcc gaatgggtga cgacgttccg caagggctgg ctcgaccgcg ccgctgtccc
     3421 cgaggccgag cgcgccgaga tcggcgccgc ggtcgccgcg agcctcgcca ccgacaccgc
     3481 cgattacgtc cgcctccgct tcatcatgag aaagcccgcc tgatgcgtta tttgccgctg
     3541 tccgatgccg accgcagtgc gatgctcgaa gtgatcggcg cgggctcggt cgacgagctg
     3601 ttccgcgacg ttccggaggc ggcgcgtctg aaggacggaa tcgccggcct gcccgaccac
     3661 gcgagcgagc tgtcggtcga gcgccagctg accgcgatgg cgcgcaagaa cctggtcgcc
     3721 ggcgaggcgc cgttcttcct cggctgcggc gcctaccgcc accatatccc ggcgagcgtc
     3781 gaccacctga tccagcgcgg cgagttcctg accagctaca cgcccgacca gcccgagatc
     3841 gctcagggca cgctgcaggt gctgttcgag ttccagaccc aggtcgcgcg actctacggc
     3901 tgcgaggtcg ccaacgcgtc gatgtacgac ggctcgaccg cctgctggga agcgatcggc
     3961 atggcccggc ggatcacgcg ccggacgaag acggtgatct cgtcgggcct ccacccgcat
     4021 tatgtcagcg tcgccaacac catggcgcgg ttcaccggcg acgtgctcga caccgcgctg
     4081 cccgagctgg ccgcggagac cgactccgaa cggcagatcg cgagcattga cggcgagacc
     4141 agcgcggtcc tcgtccaata ccccgacatc ctcggccggg tcgccgattt ggcgccgatc
     4201 gccgaggccg cgcatgccgc cggagcgctg ctgatcgcgg tggtcaccga gccggtcgca
     4261 ttggggctga tcaagtcgcc gggcgagatg ggcgccgaca tcgtcgtcgg cgagggccag
     4321 tcgatcggcg tcgggctgca ggtcggcggg ccctacgtcg gcctgttcgc atgccgcgaa
     4381 aagcacgtcc gccagatgcc ggggcggctg gccggagaga ccgtcgacgc ggaaggcagg
     4441 cgcggcttcg tcctcaccct gtcgacgcgc gagcagcata tccgacgcga gaaagcgacc
     4501 agcaacatct gcaccaactc ggggctgtgc gcgctggcct tctcggtcca cctgagcctg
     4561 ctcggcgaga aggggcttcg gcggctcgcc gagatcaacc acgcgagggc ggtcgaggtc
     4621 gcggcgcggc tcgcggcgat ccccggcgtc agcctggtca acgacagctt cttcaatgaa
     4681 ttcaccctcc ggctgccggt cgaggcgcgg ccggcggtcc gcgagctcgc cgaccgcggg
     4741 atcctgggcg gagtgtcgct cggccggctc tacccgggcg cgcaggcgct ggagaacggc
     4801 ctggtcgtcg cggtcaccga gaccgtcacc gacgaggaca ttgccgcgtt cgaggccggg
     4861 ctgaaggagg tcgtgcgatg accgtcaacc cgtcgggatg gcggccggcg gccccggtca
     4921 aggcggcccc ggctgaccag caaggcgaag gctcgaccgt gaccggcaac cgggcgctga
     4981 tgctcgagga gccgttgctg ttcgagatcg gcgacaccga gacgacgggg gtcgatttcc
     5041 cggacgtgcc ggcggcgacg actcgcctcg gcggcctcga gcgcacaggc ccgatcggcc
     5101 tcgccggcct gtcggagccc gaggcggtgc gtcattacac ccgcctcagc cgccagaatt
     5161 acgcgatcga cctcgggctg ttcccgctcg gtagctgcac gatgaagcat aacccgcgcc
     5221 tcaacgagaa gatggcgcgg cttcccggct tcgccgacat ccacccgctg cagcccgaga
     5281 gcacggtgca gggggcgctc gagctgatgg acacgctggc gcactggctc aagacgctca
     5341 ccggcatgcc ggcggtggcg ctg
//
LOCUS       DASTZO010000030         3099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1099009,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000030 DASTZO010000000
VERSION     DASTZO010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1099009"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..682)
                     /locus_tag="VFU20_00715"
     CDS             complement(<1..682)
                     /locus_tag="VFU20_00715"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU5285013.1"
                     /translation="MAEQRAPEPDRDEAPIRAPIRATIAGTKLELIESGTARLEALLE
                     LIRGAERSIRLLFYIFVGDASGTAVRDALVEAARRGVRVHVLLDGYGCAKIDPKFFQA
                     LDENGGKFCLFNPAYGRRYLVRNHQKLAIADERRAIIGGANVEDCYLTDEGPAHWRDL
                     WILVDGPMVVGAANYFDAVYRWTGREGAKLKSLRRLVTRFSDRQGPLQWHFSAPLSMR
                     TPWPSVFAR"
     gene            832..1173
                     /gene="rpoZ"
                     /locus_tag="VFU20_00720"
     CDS             832..1173
                     /gene="rpoZ"
                     /locus_tag="VFU20_00720"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEU5285014.1"
                     /translation="MARVTVEDCVDKIPNRFDLVLMAAQRARQVSGGADLTIERDRDK
                     NPVVALREIAEETVKPRDLEEAIVSNLQKVRVDEEDETDELASLSESAEALRLTAAAP
                     PRPSPSGGDYE"
     gene            complement(1222..1695)
                     /locus_tag="VFU20_00725"
     CDS             complement(1222..1695)
                     /locus_tag="VFU20_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007713347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5285015.1"
                     /translation="MDAFREAALHCPLPGAVELIGEKWAFLILRGALNGLRHFEEFQA
                     GLGIARNILSDRLGKLVAGGILERSPDPTDRRKVIYALTAKGEGLLPVMLALRQWGEQ
                     WGQGPAPVVLADRLNGRPIRRICVQAEDGRELELGDLTWLSRDHLESAPHKSGAA"
     gene            1844..>3099
                     /locus_tag="VFU20_00730"
     CDS             1844..>3099
                     /locus_tag="VFU20_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HEU5285016.1"
                     /translation="MLRQYELVEKVRSYDPDADEGLINRAYVFSMKAHGSQVRASGDP
                     YFSHPIEVAGILTDLKLDDQTIVTAILHDTIEDTLATPEEIEKHFGKDIARLVDGVTK
                     LSKIEAQSENERAAENLRKFLLALSDDIRVLLVKLADRLHNMRTLHHVPDEDKRRRIA
                     RETMDIYAPLAERIGMYEIMNEMQTLAFRELEPDAYASITRRLKLLHDQGGDIVSRIG
                     LGLQLHLADNGLEAVVMGREKHPFSIWKKMAERHISFEQLSDVMAFRVIVDDAADCYR
                     ALGLIHQRWPMVPGRFRDFISTPKRNGYRSIHTSVIHDAKMRIEIQIRSRDMHEQAER
                     GLAAHWAYKEGKPSADLRVPWIDDLVEILDHAESPEELLEHTRMAMYQDRIFAFTPRG
                     EVHQLPKGATPVDFAYAVHTKLGNQTV"
BASE COUNT          531 a         1086 c         1023 g          459 t
ORIGIN      
        1 cccgggcgaa gaccgagggc cacggcgtcc gcatgctgag cggggcgctg aaatgccact
       61 gcagcggccc ctgccggtcg ctgaaccggg tcaccagccg gcgcaggctc ttgagcttgg
      121 cgccctcgcg gccggtccag cgatagacgg cgtcgaaata attggcggcc ccgaccacca
      181 tcggcccatc gaccaagatc cacaggtcgc gccagtgcgc cgggccctcg tcggtcagat
      241 agcagtcctc gacgttggcg ccgccgatga tcgcccgccg ctcgtcggcg atcgccagct
      301 tctggtggtt gcggaccagg tagcggcggc cgtaggccgg gttgaacagg cagaacttgc
      361 cgccattctc gtccagcgcc tggaagaatt tgggatcgat cttggcgcag ccgtagccgt
      421 cgagcagcac atggacccgg accccgcgcc gggccgcctc gaccagggcg tcgcggaccg
      481 cggtccccga cgcgtcgccg acgaagatgt agaacaatag ccggatgctg cgctcggcgc
      541 cgcgaatgag ctcgagcagc gcctcgagcc gcgcggttcc gctctcgatc agctccagct
      601 tcgtcccggc gatcgtcgcc cggatcggcg cccggatcgg cgcttcgtcc ctgtccggct
      661 cgggggcgcg ttgttcggcc ataggcggca agctgtggcg gcgcggcgct cgcggcgcaa
      721 gcgttgagcg gcgccgagcg gcgccgggcg aaggccgctt tccttgacta gcggcatggc
      781 ccggcctatc tcgcggcctt cctttcaacc tgcagatttg cggagcgacg aatggcgcgc
      841 gttacggtcg aagattgtgt cgacaagatc ccgaaccggt tcgacctggt gctgatggcc
      901 gcgcagcggg cgcgccaggt gtcgggcggc gccgacctga ccatcgagcg cgaccgcgac
      961 aagaacccgg tcgtcgcgct gcgcgagatc gccgaggaga cggtcaagcc gcgcgacctc
     1021 gaggaagcga tcgtctccaa cctgcagaag gtgcgggtcg acgaagagga cgagaccgac
     1081 gagctcgctt cgctgagcga atcggccgag gcgctgcgcc tgaccgccgc cgctccgccg
     1141 cgcccgagcc cgtccggcgg cgattacgaa taagcggtac gcgcaggctg cccggtgggc
     1201 agccgggaat gccgctccgg gctaggccgc tccgctcttg tgcggcgcgc tttcgagatg
     1261 gtcgcgcgac agccaggtca ggtcgccgag ctcgagctcg cggccgtcct cggcctggac
     1321 gcagatcctc cggatcgggc gcccgttcag ccgatcggcg agcaccaccg gcgccggccc
     1381 ctgcccccat tgctcgcccc attggcgcag cgccagcatg accggcagca gcccctcgcc
     1441 cttggcggtc agcgcataga tcaccttgcg ccggtcggtc gggtcgggcg accgctcgag
     1501 gatcccgccg gcgaccagct tgccgagccg gtcggagagg atgttgcgcg cgatcccgag
     1561 ccccgcctgg aactcctcga aatgccgcag cccgttgagg gccccgcgaa ggatcaggaa
     1621 cgcccatttc tcgccgatca gctcgaccgc gcccggcagc ggacagtgca gcgccgcctc
     1681 gcggaacgca tcgatgccgg tggtgacctt ttcgctcggc aagccgccgt cctcccgtcg
     1741 gagcaggaaa gcacaagcgc ccggcgaagc aaagttgcaa tttggaaccg gtgcggcccg
     1801 tccgcacttg cgcacgtggc gccgctcgcc ccatctaggg accgtgcttc gacaatatga
     1861 actggtcgag aaggttcgct cctatgaccc cgatgccgac gaggggctga tcaaccgcgc
     1921 ctacgtgttc tcgatgaagg cccacggctc gcaggtccgg gcgagcggcg acccctattt
     1981 cagccatccc atcgaggtcg ccgggatcct caccgacctg aagctcgacg accagaccat
     2041 cgtcaccgcg atcctccacg acacgatcga ggacacgctc gcgaccccgg aggaaatcga
     2101 gaagcatttc ggcaaggaca tcgcccggct ggtcgacggg gtgaccaagc tcagcaagat
     2161 cgaggcgcag tccgaaaacg agcgcgccgc ggagaacctc cgcaagttcc tgctcgcttt
     2221 gtccgacgac ataagggtgc tgctggtcaa gctcgccgac cggctgcaca acatgcgcac
     2281 gctccaccac gtgcccgacg aggacaagcg ccgccggatc gcccgcgaga cgatggacat
     2341 ctacgctccg ctcgccgagc gcatcggcat gtacgagatc atgaacgaga tgcagacgct
     2401 ggccttccgg gagctcgagc cggacgccta tgcgtcgatc acccggcgat tgaagctgct
     2461 ccacgaccag ggcggcgaca tcgtcagccg gatcggcctc gggctgcagc tgcacctcgc
     2521 cgacaacggc ctcgaggcgg tggtcatggg ccgcgagaag cacccgttct cgatctggaa
     2581 gaagatggcc gagcgccaca tcagcttcga gcaattgtcg gacgtgatgg ccttccgggt
     2641 gatcgtcgac gacgccgccg actgctaccg ggcgctgggc ctgatccacc agcgctggcc
     2701 gatggtgccg ggccgtttcc gcgatttcat ctcgaccccg aagcgcaacg gctaccgctc
     2761 gatccacacc tcggtgatcc acgacgccaa gatgcgcatc gaaatccaga tccggtcgcg
     2821 cgacatgcac gagcaggccg agcgcggcct cgccgcccac tgggcgtaca aggaaggcaa
     2881 gccgtcggcc gacctgcgag tgccgtggat cgacgatctg gtcgagatcc tcgaccatgc
     2941 cgagagcccc gaggagctgc tcgagcacac gcggatggcg atgtatcagg accggatctt
     3001 cgctttcacg ccgcgcggcg aagtgcatca gctccccaag ggcgcgactc cggtggactt
     3061 tgcctatgcc gtgcatacca agctcggcaa ccagaccgt
//
LOCUS       DASTZO010000031         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1073172,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000031 DASTZO010000000
VERSION     DASTZO010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073172"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="VFU20_00735"
     CDS             complement(<1..499)
                     /locus_tag="VFU20_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEU5285017.1"
                     /translation="MMTDLAIYDLDRTLTRRATYTPFLIHCALRRAPWRLLLLPVVLI
                     SVLAYAARIIDRGRLKEINHHLLLGRWIHPKDLKPLVDSFAEATVARNVRPGARKAIA
                     RDKAQGRRLVLATASYRLYADAIAERLGFDDVIGTGSVVGLDERIRSRIHGENAYGEA
                     KLRMIA"
     gene            complement(496..1263)
                     /locus_tag="VFU20_00740"
     CDS             complement(496..1263)
                     /locus_tag="VFU20_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5285018.1"
                     /translation="MSGWTALVLAGSRPGRDPFAASHGTDLKALIPVGGEPMVRRPVA
                     ALLASERIGQVRVLAQQAPRIAQALPADPRLTVEPSAGTIATTLERVCADPATRWPLL
                     VTTADHALLDTAMIDDFCDRSAQADIAIAVVERKALLDRLPATRRTWIAFRGGAWSGA
                     NLFLLRSPKVAPAIALWRSVEQDRKKGWRLLWAMGPALFLGSVLRLLTLDQALGRIGR
                     RLGLDVRAVAMANPLAAVDVDKESDHVLVEAIVAGRA"
     gene            complement(1274..>2174)
                     /locus_tag="VFU20_00745"
     CDS             complement(1274..>2174)
                     /locus_tag="VFU20_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013831569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU5285019.1"
                     /translation="LAGRPVLANVAAGGPALAPVAEALERGDMAALAGLDPVAAETVE
                     LSYRKLRKRDRPFVLPLDPADPQPVERAAYDAAYKGVTDVLTLYLWRKPAFHVTRWAA
                     EAGLTPNMVTGIGFAFCLLAFWLFWIGQYWPGIAAGLVFMVLDTVDGKLARCTGTSSE
                     WGNIFDHGIDLVHPPFWWWAWLHGLHAYGRPLEPIYATMLLWAIIGGYVAQRLIEGIF
                     LRRHGMHIHVWRPVDSRFRLITARRNPNMVILFAALLFGRPDVGIELVALWTIVSLIF
                     HAVRMAQATVTSEQGGKVVSWLA"
BASE COUNT          340 a          743 c          811 g          280 t
ORIGIN      
        1 cggcgatcat ccgaagcttg gcctcgccat aggcgttctc gccgtggatc cgcgagcgga
       61 tgcgctcgtc gaggccgacc accgagcccg tgccgatgac gtcgtcgaag ccgagccgct
      121 cggcgatcgc gtcggcgtag agccggtagg aggcggtcgc gagcaccagc cggcggccct
      181 gggccttgtc gcgggcgatg gcttttcgcg cgcccggccg gacgttgcgg gcgaccgtcg
      241 cctcggcgaa gctgtcgacc agcggcttca aatccttcgg gtggatccag cggccgagca
      301 gcaggtgatg gttgatctcc ttcaaccgcc cgcggtcgat gatccgcgcc gcgtaggcga
      361 ggaccgaaat caggaccacc ggcagcagca gcagccgcca cggcgcccgg cgcagcgcgc
      421 agtggatcag gaacggagtg taggtcgcgc ggcgcgtcag cgtccggtcg aggtcgtaga
      481 tggcgaggtc ggtcatcatg cgcggccggc gacgatggct tcgaccaaca cgtggtcgct
      541 ttccttgtcg acgtcgaccg cggcgagcgg gttcgccatc gcgaccgcgc ggacgtcgag
      601 gccgagccgg cggccgatcc ggcccagcgc ctggtcgagg gtcagcagcc gaagaacgct
      661 gccgaggaac agcgccgggc ccatcgccca caacagccgc cagcccttct tgcggtcctg
      721 ctcgaccgag cgccacagag cgatggcggg cgcaaccttc ggcgagcgta gcaggaacag
      781 gttcgcgccg ctccaggcgc cgccccggaa ggcgatccag gtgcgccggg tcgccggcag
      841 gcggtcgagc agcgccttgc gctcgacgac cgcgatggcg atgtcggcct gcgccgagcg
      901 gtcgcagaaa tcgtcgatca tcgccgtatc gagcagcgca tggtcggcgg tcgtgaccag
      961 cagcggccag cgcgtcgcgg ggtcggcgca gacgcgctcg agggtcgtgg cgatggtccc
     1021 ggccgacggc tcgaccgtaa gccgcgggtc ggcgggcagg gcttgggcga tccggggcgc
     1081 ctgctgggcg agcacccgca cttggccgat ccgctcgctc gccagcaggg cggcgaccgg
     1141 gcggcggacc atcggctcgc cgccgaccgg gatcagcgcc ttcagatcgg tgccgtggct
     1201 cgccgcgaac gggtcccggc ccggccgtga cccggcgagc accagcgccg tccagccgct
     1261 cattcgagcc cggtcacgcc agccacgaga cgaccttgcc gccctgctcg gacgtcacgg
     1321 tcgcctgcgc catccgcacc gcgtggaaga tcaaggagac gatggtccac agagcgacca
     1381 gctcgatgcc gacgtcgggc cgcccgaaca gcagagcggc gaacaggatc accatgttcg
     1441 ggttgcgccg cgcggtaatc agccggaagc ggctgtcgac cggcctccag acgtggatgt
     1501 gcatgccgtg gcggcgcagg aaaatgccct cgatcaggcg ctgcgcgaca tagccgccga
     1561 tgatcgccca caagagcatg gtcgcgtaga tcggctccag cggccggccg taggcgtgca
     1621 agccgtgcag ccacgcccac caccagaagg gcgggtggac gaggtcgatg ccgtggtcga
     1681 agatgttgcc ccattccgac gaggtgccgg tgcagcgcgc gagcttgccg tcgaccgtgt
     1741 cgagcaccat gaagaccagt ccggcggcga tcccgggcca atattgcccg atccagaaca
     1801 gccagaaggc gagcaggcag aaagcgaagc cgatgccggt gaccatgttc ggggtcaggc
     1861 ccgcctcggc cgcccagcgg gtcacatgga aggcgggctt gcgccacaga tagagcgtca
     1921 ggacgtcggt gacgcccttg taggcggcgt cgtaggcggc gcgctcgacg ggttgcgggt
     1981 cggccgggtc gagcggcagg acgaacgggc ggtcgcgctt gcgaagcttg cggtagctga
     2041 gctcgaccgt ctcggcggcg accgggtcga ggccggcgag cgccgccatg tcgccgcgct
     2101 cgagcgcctc ggcgaccggg gcgagggccg ggccgccggc cgcgacattg gcgagcaccg
     2161 gccggccggc cagg
//
LOCUS       DASTZO010000032         7039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_524797,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000032 DASTZO010000000
VERSION     DASTZO010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7039
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524797"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VFU20_00750"
     CDS             <1..222
                     /locus_tag="VFU20_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU5285020.1"
                     /translation="APIDILADRTARRDTRPLLRNGDPKGTLERLAEERRPSYASAHI
                     HVKSGDGAHRDVVDSILKALEDHLGGSDS"
     gene            complement(219..1955)
                     /locus_tag="VFU20_00755"
     CDS             complement(219..1955)
                     /locus_tag="VFU20_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5285021.1"
                     /translation="MLGAALLFVTLFRKLGLGATLGYIVGGAVIGPYALGLIGDPEAV
                     MRVSEIGIAFLLFLVGLELHPSRLWRLRKDIFGLGLAQVVLCGLALSALIYWGLGLSI
                     QASLALGLPLGLSSTAQVLPMLRSSGQLNTRRGERAFSVLLFQDLSIVPMITIIAAMS
                     RAPLDPDQPSGGMLALYTVAAIVGLVLAGRFILNPLFRMIGRFGERELFVVAGLFAII
                     ASASLMETLHLSVALGAFIAGVVLAESPYRHELESDVEPFRSILLGMFFLSVGMLLDL
                     DAIAAAPLFVFGAAAGIVAVKAVLIGALARAFGMKVGRAIWLGLLLSQGGEFGFVLFG
                     QAAAAQLIEPELATLFAAIVTVSMIFTPFLMRVTEWVLERVPDSEVALQGPEFSPTTN
                     AIVVGYGRFGQTVGQMLMAKRIPVTIIDSKPAQIELSESFGTKVYYGDGTRVDLLRTA
                     GAETAEAILFCQDGQDLDRAKLEAVLEAFPQAQVMVRAYDRRQVIEFDGLDIGLIQRE
                     LFESAVLMGREALLKLGIDRREADRVEAAYRQRDAERLERQSATGDLRAARDRMFRPD
                     SSLPDEQSRDPA"
     gene            complement(2006..2425)
                     /locus_tag="VFU20_00760"
     CDS             complement(2006..2425)
                     /locus_tag="VFU20_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HEU5285022.1"
                     /translation="MNAPLYTIEILRLAGSLPDPAHLERVDGAAQLRSPTCGSTVRTE
                     VRMDRGVVGAISQQVRACAFGQAAAALVAQHAVGRSRDEVAAMLAGLSEWLEGARDEP
                     GGWGLEALAPARPRTSRHGAIVLPLRALIAAIEDTGA"
     gene            complement(2422..3732)
                     /gene="cysS"
                     /locus_tag="VFU20_00765"
     CDS             complement(2422..3732)
                     /gene="cysS"
                     /locus_tag="VFU20_00765"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU5285023.1"
                     /translation="MIRLYDTMAREKREFVPGDPRRITMYVCGPTVYGRAHIGNARPA
                     VVFDTLARLIRHQFGDDCLVYARNVTDVDDKIIAAADKEGIEPAAVTERYERHYNEDM
                     GALGVRDPDIAPHATAEIGPMVAMIERLVELGHAYEAEGHVLFHVPSDPEYGALSRRD
                     RDAMVAGARVEVAPYKRDPADFVLWKPSDAGVIGWDSPWGRGRPGWHIECSAMIRAHL
                     GETIDIHGGGLDLIFPHHENEIAQSRTAHGGKPLARYWVHNGFVDMGAEKMSKSLGNI
                     VTVGELLEQGHRGETLRLALLSAHYRQPLGWTEAGIAQAKTMLDRLFRAAGDVAPGEI
                     DDGVLEALADDLNTPLALTRLMAIDDPGAVKASAALLGLLEQDAEAWFRGDSSGMDVE
                     ARIAERAAAKSRRDFATADRIRDELKAEGIVLEDGPGGTTWRRE"
     gene            complement(3729..4247)
                     /locus_tag="VFU20_00770"
     CDS             complement(3729..4247)
                     /locus_tag="VFU20_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEU5285024.1"
                     /translation="MSALDIFVILLLGGGAIIGFVRGFVHEVLSLFAWVVAIAMLKLF
                     HTQLSEGLTDPVGTAPGAAVVAFALLFVPSFVLVKLLARSIGGKTRKSLLGPVDRVLG
                     GGFGMIKGLLGATLFFLLANLATDMLYGPEAERPEWMTSSRTYPLLNASGLAIVDWVE
                     ARRLRPKAVGEE"
     gene            complement(4291..5655)
                     /gene="radA"
                     /locus_tag="VFU20_00775"
     CDS             complement(4291..5655)
                     /gene="radA"
                     /locus_tag="VFU20_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010187035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU5285025.1"
                     /translation="MAKPRTRYVCQACGSVSSRWQGQCADCAEWNTLVQEAAEVSSIF
                     AAKHNLRGGGRTIELVGLDSEAALPARFATGIAEFDRAVGGGIVPGSAMLVGGDPGIG
                     KSTLLLQVGARLAEQGQGVVYVSGEESAEQIRLRAVRLGLGKSPVRLAAATSVRDILT
                     TLEGAPPALLVIDSIQTMHSDLIEGAPGTVSQVRASAQELVRFAKEQGSAVVLVGHVT
                     KDGAIAGPRVLEHMVDTVLSFEGERSHQYRILRAMKNRFGGTDEIGVFAMEEAGLSQV
                     PDPSALFLTRRSEPVSGTSVFPALEGTRPVLVEIQALTVRLASGATPRRAAVGWDSGR
                     LAMILAVLEARCGLSFATAEVYLNVAGGYRLSDPAADLAVAAALVSALAERPVPGECV
                     VIGEIALSGEVRPVAHAALRLKESAKLGFETAWVPPGIKSGSGIALAEFANLRALADH
                     ILGR"
     gene            5735..6556
                     /locus_tag="VFU20_00780"
     CDS             5735..6556
                     /locus_tag="VFU20_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5285026.1"
                     /translation="MPYATARDGTRIYYKDWGQGRPVVLIHGWPLSSDTFDDAALALA
                     ENGYRAIAHDRRGFGRSDQPWGGYDYDTFADDVAAVLEEAGIGQPIALAGFSMGGGEV
                     ARFLSRQRAHQVSHAVLIGSVVPYLLQTDDHPEGAPRDVFDGMIGGIRKDRAAFFRAF
                     FKDFFGVSWTTPVSDAVLDDAWRQAMNAGLWPTLACVRAFSETDFRPDLDAFSMPTLI
                     IHGVDDRTVPIDLTARAAAEAIPGSRLIEYDDAAHGLFASHKDRLVADLLEFLGG"
     gene            6563..>7039
                     /locus_tag="VFU20_00785"
     CDS             6563..>7039
                     /locus_tag="VFU20_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5285027.1"
                     /translation="MSQSPLAIPDFRLYWAARFTAVLATMGMVVIIGYQVYDVARADY
                     GMSIRQASLQLGLLGLAQFVPLALLTPLAGWVADRFERRTVARLANGIDMAVALTLAW
                     CTASDLLTLPLLFGLAALHGIARVFVGPAMTAIAPNIVPPELLPRAIAMSSIAWQSA"
BASE COUNT         1069 a         2625 c         2331 g         1014 t
ORIGIN      
        1 gcgccgatcg acatcctcgc cgaccgcacc gcgcggcgcg acacccggcc gctgctccgc
       61 aacggcgacc cgaagggcac gctcgagcgc ctcgccgaag aaaggcggcc gtcctacgcg
      121 tcggcccaca tccatgtgaa aagcggtgac ggagcgcatc gcgacgtggt cgattcgatc
      181 ctcaaggcgc tcgaggacca cctcggcgga agcgactcct aggccgggtc cctcgactgc
      241 tcgtccggaa gcgaggaatc gggcctgaac atccggtcgc gggccgcgcg caggtcgccg
      301 gtcgcgctct ggcgctcgag ccgctcggca tcccgttgcc gatacgcggc ctcgacccgg
      361 tcggcctcgc ggcggtcgat cccgagcttg agcagcgcct cccggcccat cagcacggcg
      421 ctctcgaaca gctcgcgctg gatcaggccg atgtcgagcc cgtcgaactc gatcacctgc
      481 cgccggtcgt aggcgcggac catcacctgc gcctgcggga acgcctcgag cacggcttcg
      541 agcttcgccc ggtccaggtc ctggccgtcc tggcagaaca ggatcgcctc ggcggtctcg
      601 gcgccggcgg tgcgcagcaa atcgacgcgg gtgccgtcgc cgtaatagac cttggtcccg
      661 aacgattcgc tgagctcgat ctgcgccggc ttgctgtcga tgatcgtcac cgggatccgc
      721 ttggccatca gcatctgccc gacggtctgc ccgaaccgcc cgtagccgac gacgatggcg
      781 ttggtcgtcg gcgagaactc gggcccttgc agggcgacct cactgtcggg cacccgttcg
      841 agcacccatt cggtgacccg catcaggaac ggcgtgaaga tcatcgacac ggtgacgatc
      901 gccgcgaaca gggtcgccag ctcgggctcg atcagctgcg cggcggccgc ctggccgaac
      961 agcacgaagc cgaattcgcc gccctggctg agcagcaggc cgagccagat cgcccggccg
     1021 accttcatcc cgaacgcccg agcgagggcg ccaatcagca ccgccttgac ggccacgatg
     1081 cccgccgccg ccccgaacac gaacaatggg gcggccgcga tcgcgtcgag gtcgagcagc
     1141 atcccgaccg acaggaagaa catcccgagc aggatcgagc ggaacggctc gacgtcgctt
     1201 tcgagctcgt ggcggtaggg cgattcggcg agcaccacgc cggcgatgaa cgctcccagc
     1261 gcgaccgaca ggtggagagt ctccatcagc gacgcgctgg cgatgattgc gaacaggccg
     1321 gcgaccacga acaattcgcg ctctccgaag cggccgatca tccggaacaa agggttgagg
     1381 atgaagcggc cggcgagcac caggccgacg atcgccgcga ccgtgtacag agcgagcatc
     1441 ccgcccgacg gctggtcggg gtcgagaggc gcccgcgaca tcgccgcgat gatggtgatc
     1501 atcggcacga tcgacaggtc ctggaacagc aggaccgaga acgcccgttc gccgcggcgc
     1561 gtgttgagct gccccgagga gcgcagcatc ggcaacacct gcgcggtcga cgacaggccg
     1621 agcggcagcc cgagagcgag gctcgcctgg atgctgaggc cgagccccca atagatcagc
     1681 gcgctgagcg ccagcccgca cagcaccacc tgcgccaggc ccaatccgaa aatgtccttg
     1741 cgcagccgcc acaagcggct cgggtgaagc tcgagcccga ccaggaacag caggaaggcg
     1801 atgccgatct cggacacccg catcaccgcc tcggggtcgc cgatcaagcc gagcgcatag
     1861 gggccgatca ccgccccgcc gacgatgtag cccagcgtcg cgccgaggcc gagcttgcgg
     1921 aacagggtga cgaacagcag cgccgcgccg agcatgaagg cgccgttgag aagcatcccg
     1981 ccggtcgccg cgacttcggc ctggttcaag ctcccgtatc ctcgatcgcc gcgatcagcg
     2041 cccgtagcgg cagcacgatc gccccgtgcc gcgaggtccg cggccgcgcc ggcgcgagcg
     2101 cctcgagccc ccagccgccg ggctcgtcgc gcgctccctc gagccactcg ctcaggccgg
     2161 cgagcatcgc cgcgacctcg tcccggctcc gcccgaccgc gtgctgcgcg accagcgccg
     2221 cggcggcctg gccgaacgcg cacgcccgca cttgctgcga aatcgcgccg acgacgccac
     2281 ggtccatccg cacctcggtg cgcaccgtgc tgccgcaggt cggcgagcgc agctgcgccg
     2341 ccccgtcgac ccgctcgagg tgcgccggat cgggcagcga ccccgccagc cgcaggatct
     2401 cgatggtgta cagcggcgcg ttcattccct gcgccaggtg gtgccgcccg gcccgtcttc
     2461 caagacgatc ccctcggctt tcagctcgtc gcggattcgg tccgcggtcg cgaagtcgcg
     2521 gcggctcttg gccgcggcgc gctcggcgat ccgcgcctcg acgtccattc cgctcgaatc
     2581 cccgcggaac caggcttccg catcctgttc gagcaagccg agcagcgccg cgctcgcctt
     2641 gaccgccccc ggatcatcga tcgccatcag ccgggtcagc gccagcggcg tgttgagatc
     2701 gtcggcaagc gcctcgagca ctccgtcatc gatttccccc ggcgccacat cgcccgctgc
     2761 ccggaagagc cggtcgagca tcgtcttcgc ctgggcgatc cccgcctcgg tccagccgag
     2821 cggctggcgg tagtgcgcag acagcagcgc cagccgcagc gtctcgccgc ggtgcccctg
     2881 ctccagcagc tccccgacgg tgacgatgtt gccgagcgac ttcgacatct tctcggcgcc
     2941 catgtcgacg aagccgttgt gcacccagta gcgcgcgagc ggcttgccgc cgtgcgcggt
     3001 ccggctctgc gcgatttcgt tctcgtggtg cggaaaaatg aggtcgagcc cgccgccgtg
     3061 gatgtcgatc gtctcgccga gatgcgcccg gatcatcgcc gagcattcga tgtgccagcc
     3121 cggccgcccg cggccccacg gcgagtccca gccgatcacg cccgcgtcgc tcggcttcca
     3181 cagcacgaaa tcggccgggt cgcgcttgta cggggcgacc tcgacccgcg ccccggcgac
     3241 catcgcgtcg cggtcgcggc ggctcaatgc gccgtattcc gggtccgacg ggacgtggaa
     3301 cagcacgtgg ccttccgcct cgtaagcgtg gccgagctcg accagccgct cgatcatcgc
     3361 caccatcggc ccgatctcgg cggtcgcgtg cggcgcgatg tcggggtcgc ggacgccgag
     3421 cgcgcccatg tcctcgttgt agtgccgctc gtagcgctcg gtgaccgccg ccggctcgat
     3481 gccttccttg tccgccgcgg cgatgatctt gtcgtcgacg tcggtgacgt tgcgcgcgta
     3541 gaccaggcag tcgtcgccga actggtggcg gatcagccgg gcgagcgtgt cgaacaccac
     3601 cgccgggcgg gcgttgccga tgtgcgctcg cccgtagacc gtcggcccgc acacatacat
     3661 ggtgatgcgc ctggggtcgc cgggcacgaa ctcgcgcttc tcccgcgcca tcgtgtcgta
     3721 tagccggatc attcctcgcc caccgccttg ggcctgagcc gccgcgcctc gacccagtcg
     3781 acgatcgcca ggccgctcgc gttgagcaag ggataggtcc gcgagctggt catccactcg
     3841 ggccgctcgg cctcggggcc gtagagcatg tcggtggcaa ggttggcgag caggaagaac
     3901 agggtcgccc cgagcaaccc cttgatcatc ccgaagccgc cgccgagcac ccggtcgacc
     3961 gggccgagca gcgacttcct ggtcttgccg ccgatcgagc gggcgagcag cttgaccaac
     4021 acgaagctgg gtacgaacag cagggcgaag gcgaccaccg cggcgccggg cgcggtcccg
     4081 accgggtcgg tcagcccctc cgacagctgg gtgtggaaca gcttgagcat ggcgatcgcg
     4141 accacccagg cgaacagcga cagcacctcg tggacgaagc cgcggacgaa tccgatgatc
     4201 gctccgccgc ccagcagcag gatgacgaag atgtcgagcg cgctcatcgc gctgcggcta
     4261 gcgggctatc cgccgcttcg ccagcccgga ttagcggccg aggatatggt cggcgagcgc
     4321 gcgcagattg gcgaactcgg cgagcgcgat gccgctgccg cttttgatcc cgggcggaac
     4381 ccaggcggtc tcgaagccga gcttggccga ttccttaagc ctgagcgccg cgtgcgcgac
     4441 cggccgcacc tcgcccgaca gcgcgatctc gccgatcacc acgcactcgc ccggcaccgg
     4501 gcgttctgcg agagccgata ccaaggcggc tgcgacggcg aggtcggcgg ccgggtccga
     4561 cagccggtac ccgccggcga cgttcagata gacttcggcg gtcgcgaagc tcagcccgca
     4621 gcgcgcctcc agcaccgcga ggatcatcgc cagccggccc gaatcccagc cgaccgccgc
     4681 gcgccgcggg gtcgcgccgc tcgccagccg aaccgtcagc gcctggatct cgaccagcac
     4741 cggccgggtg ccctccaacg ccgggaagac gctggtgccc gacaccggct cggagcggcg
     4801 ggtgaggaac aaagccgacg ggtcggggac ctggctcagc cccgcttcct ccatcgcgaa
     4861 cacgccgatc tcgtcggtgc cgccgaagcg gttcttcatc gcccgaagga tccggtactg
     4921 gtggctgcgc tcgccctcga agctcagcac cgtgtcgacc atgtgctcga gcacccgcgg
     4981 gccggcgatg gcgccgtcct tggtgacgtg gccaaccagc accaccgcgc tgccctgctc
     5041 cttggcgaag cgcaccagct cctgggccga ggcgcggacc tggctgaccg tccccggcgc
     5101 cccctcgatc aggtcggagt gcatggtctg gatggaatcg atcaccagca gcgccggcgg
     5161 cgcgccctcg agcgtggtca ggatgtcgcg caccgaagtc gcggccgcga gccggaccgg
     5221 cgacttgccg agccccagcc gcaccgcgcg cagccggatc tgctcggccg attcctcgcc
     5281 cgacacataa accacgccct gcccctgctc ggcgagcctg gcgccgacct ggagaagcag
     5341 cgtcgacttg ccgatgcccg ggtcgccgcc gaccagcatc gccgatcccg gcacgatgcc
     5401 gccgccgacc gcccgatcga actcggcgat cccggtcgcg aagcgggccg gcagcgcggc
     5461 ctcgctgtcg agcccgacca gctcgatggt ccggccgccg ccgcgcagat tgtgcttggc
     5521 ggcgaagatg ctcgacactt cggccgcttc ctggaccaac gtgttccact cggcgcaatc
     5581 ggcgcactgg ccctgccagc ggctgctcac cgacccgcag gcctggcaca cgtagcgggt
     5641 cctcggcttg gccatcctcg accgttagcg aatcgctccg ctttgtccta ggggcgtcgc
     5701 ttgcaggata agggggcggg gaacaggagg acatatgccc tacgcgaccg cccgggacgg
     5761 gacccggatc tattacaagg attggggcca gggccgcccg gtcgtgctaa tccacggctg
     5821 gccgctttcc agcgacacgt tcgacgacgc cgcgctggcg ctggccgaaa acggctatcg
     5881 ggcgatcgcc cacgaccgcc gcggcttcgg ccgctccgac cagccgtggg gcggctacga
     5941 ttacgacacg ttcgccgacg acgtcgccgc agtgctcgag gaggcgggga tcggccagcc
     6001 gatcgccctc gccggcttct cgatgggcgg cggcgaggtc gcccgcttcc tgtcgcgcca
     6061 gcgtgcccac caggtcagcc acgcggtcct gatcgggtcg gtggtgccct atctgctcca
     6121 aaccgacgac catcccgagg gcgctcccag ggacgtgttc gacggcatga tcggcggcat
     6181 ccgcaaggac cgcgccgcct tcttccgcgc ctttttcaag gacttcttcg gcgtttcgtg
     6241 gacgacgcca gtcagcgacg ccgtgctcga cgacgcctgg cgccaggcga tgaacgccgg
     6301 cctgtggccg accctcgcct gcgtccgggc cttctcggag accgacttcc ggccggacct
     6361 cgacgccttc tcgatgccga ccctgatcat ccacggcgtc gacgaccgca cggtgccgat
     6421 cgacctgacc gcgcgcgccg ccgccgaggc gatccccggc tcccggctga tcgaatatga
     6481 cgacgccgcc cacggcctgt tcgccagcca caaggaccgg ctggtcgccg acctgctcga
     6541 attcctcggc ggctaagcgc gcgtgagtca gtcgccgctc gcgatcccgg atttccggct
     6601 ctactgggcg gcgcggttca ccgcggtgct cgcgaccatg gggatggtcg tgatcatcgg
     6661 ctaccaggtc tacgacgtcg cccgcgccga ctatggcatg tcgatccgcc aggcgtcgct
     6721 gcagctcggt ctgctcggtc ttgcccagtt cgtcccgctc gcccttctga ccccgctcgc
     6781 cggctgggtc gccgaccggt tcgagcggcg gaccgtcgcc cggcttgcca acggcatcga
     6841 catggccgtc gccctgacgc tcgcctggtg caccgcgagc gacctgctga ctctgccgct
     6901 cttgttcggc ctcgccgctc tgcatggcat cgcccgggtt ttcgtcggcc cggcgatgac
     6961 cgcgatcgct cccaacatcg ttccgccgga gctgctcccg cgggcgatcg cgatgagctc
     7021 gatcgcctgg cagtcggca
//
LOCUS       DASTZO010000033         9343 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_289992,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000033 DASTZO010000000
VERSION     DASTZO010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 9343)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9343)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9343
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_289992"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..1995
                     /gene="uvrC"
                     /locus_tag="VFU20_00790"
     CDS             46..1995
                     /gene="uvrC"
                     /locus_tag="VFU20_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU5285028.1"
                     /translation="MARTPAGTPPHPKGAERFHEERATYALKGSAQPDLEAGVAAIRE
                     VVRTLKPKPGVYRMHDAKGEVLYVGKARALKSRVTNYTQVKNLSKRLQRMVAQTRSMT
                     IVTTNNEAEALLLEAQLIKRYRPAYNVLLRDDKSFPFILLREDHPFPRVQKHRGARNV
                     KRGHYYGPFASAGSVTRTLNALQKLFLLRSCSDSFFANRSRPCLLYQIRRCSAPCVGR
                     IGEADYAALVEDAKAFLAGKSTGVQARLGKAMAEAAEAQDYELAAVYRDRLRALTYIQ
                     GSQTVHAEGLGDGDIFALACKAGAMSIQAFFIRGGQNWGHRAFFPAHTADVPEAEVLS
                     SFLVQFYEDMPPPRRILVDRELPDRELLEEALCERSGHKVSIDQPRRGGRAKLIEQAR
                     RNAEEALERRMAETTSQAKVLRELADTFELPDVPRRIEVYDNSHIMGTSPTGAMIVAG
                     PEGFRKNNYRKFNIKRPETAPGDDFAMMREVLERRFARLEKEDPDRASGEWPDLLIID
                     GGKGQLSAACEMMENAGVHDIPVIAVSKGPDRNAGRETFHLPGGRELTLPPNSALLFY
                     LQRLRDEAHRFAIGTHRAKRSKSLTTSTLDDVPGIGPNRKRALLMHFGTARAVKGAAL
                     DDLERAPGISKAMARQLYDYFHPRG"
     gene            2146..2883
                     /gene="recO"
                     /locus_tag="VFU20_00795"
     CDS             2146..2883
                     /gene="recO"
                     /locus_tag="VFU20_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU5285029.1"
                     /translation="MRIDVPAIVCALRAHGEHGAVVRLLTAEHGLQAAYVRGARGRRM
                     RPVLIAGNLVAATLSARTDSQLPQASVELVHSRAPLLGEPLPAAAIEWATALTATALP
                     EGQPYPRLYSALDALLTAIEAAPSASGWGAALVRYELLLLAELGFGLDLETCAVTGTK
                     DDLVAVSPGSGRAVSAAQAEPYAGKLLPLPRFVREGGAASWTDIAHGLDLTGHFLMRD
                     LLTQKSVVLGEARERLVDRLRRAGGLD"
     gene            2946..3338
                     /gene="apaG"
                     /locus_tag="VFU20_00800"
     CDS             2946..3338
                     /gene="apaG"
                     /locus_tag="VFU20_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HEU5285030.1"
                     /translation="MAMLFPNVATTRDISVRVAVSYLAEQSDPSSERWFWSYHVRIEN
                     GSDRAVQLLSRSWRIIDGRGTVHEVHGEGVVGEMPLIAPGGSFDYVSGCPLDTPSGSM
                     SGSYRMVDEDGNAFDIAIPRFTLLSSVN"
     gene            complement(3335..3847)
                     /gene="nrdR"
                     /locus_tag="VFU20_00805"
     CDS             complement(3335..3847)
                     /gene="nrdR"
                     /locus_tag="VFU20_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HEU5285031.1"
                     /translation="MRCPFCAHEDSQVKDSRPSEDGAAIRRRRQCEACGARFTTFERV
                     QLRDLTVVKKTGEREPFDRAKLERSIAIACRKRDIAPDRIERLVSSIQRQLETRGDEV
                     QAVQIGEAVMDGLNAIDHVAYIRFASVYKDFTEPGDFADIAGKVGEQPPARAEPRRKA
                     EPPPAEDKLL"
     gene            complement(3851..5158)
                     /gene="glyA"
                     /locus_tag="VFU20_00810"
     CDS             complement(3851..5158)
                     /gene="glyA"
                     /locus_tag="VFU20_00810"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU5285032.1"
                     /translation="MASAAKLNEVQPEGFFTRRLADADPAVADAVRDELSREQTQIEL
                     IASENIVSRAVLEAQGSVLTNKYAEGYPGRRYYQGCEPSDAVETLAIERAKQLFGCGF
                     ANVQPHSGAQANGAVMLALLKPGDTILGMSLAAGGHLTHGAPPAQSGKWFNAIQYGVR
                     PDDHRVDFDEVERLAREHKPKLIIAGGSAYPRHFDFARFRAICDEVGAILMVDMAHFA
                     GLVAAGEHPSPFGHAHVVTTTTHKTLRGPRGGMVLTDDEAIAKKINSAVFPGLQGGPL
                     MHVIAAKAVAFGEALKPGFKTYAKAVIANAKMLAGRLKERGADLVAGGTDTHLALVDL
                     RPLKLTGRDADESLERAGITCNKNGVPFDPLPPVQTSGIRVGSPAGTTRGFGEAEFRD
                     IADMVADVLDGLGANGLENNGAVEREVNGRVRELCARFPIYQD"
     gene            complement(5158..5604)
                     /gene="rpiB"
                     /locus_tag="VFU20_00815"
     CDS             complement(5158..5604)
                     /gene="rpiB"
                     /locus_tag="VFU20_00815"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HEU5285033.1"
                     /translation="MRIALASDHAGFALKRELAAWLSELGYEVADLGTNDTESVDYPE
                     FGARLANAVATGAAERGIAVCGSGIGISIAVNRNPKCRCARVDEPVSAALAREHNDAN
                     VLALGGRLIGSDMAKACVAAFLGTDFAGGRHQRRVDQLSSLLQDSD"
     gene            complement(5654..6868)
                     /gene="hemA"
                     /locus_tag="VFU20_00820"
     CDS             complement(5654..6868)
                     /gene="hemA"
                     /locus_tag="VFU20_00820"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HEU5285034.1"
                     /translation="MDYDRIFQSAIDRLHDEGRYRVFIDILRTKGSFPDARCFAGHNG
                     PKPIIVWCSNDYLGMGQHPAVVEAMETALHDVGAGSGGTRNIGGNTHYHVDLEAELAA
                     LHGKEAALLFTSGYVSNEAALSTLGKLLPGCVIFSDELNHASMIAGIKNSGCEKRVFR
                     HNDLAHLEQLLSEADSDAPKLIAFESVYSMDGDIAPIASICDLAERYGALTYLDEVHA
                     VGMYGEHGGGISERDAVADRITLIEGTLGKAFGVMGGYIAADRNIVDCIRSYAPGFIF
                     TTSLSPVLVAGALASVRHLKQSSVEREAQHQAAAQLKARFRDAALPLMPSVTHIVPLL
                     VGCPVKAKRISDILLAEYGLYVQPINYPTVPRGTERLRFTPGPCHTAAMMDELTAALV
                     EIWDRLELKLAA"
     gene            complement(6951..7742)
                     /gene="murI"
                     /locus_tag="VFU20_00825"
     CDS             complement(6951..7742)
                     /gene="murI"
                     /locus_tag="VFU20_00825"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU5285035.1"
                     /translation="MNPAAPILFFDSGVGGLSVLTPTRALLPSAPFVYVADNAGFPYG
                     ERSESDIASRIPALLGRVVERFRPRLAVIACNTASTIALDTVRSALELPVVGTVPAIK
                     PASEMSKTRVIGVLGTQATVRQPYVDDLAARFASDCRVIRHGSPELVELAEAKLAGDE
                     VALDAVRAAVQPMFDAGHGQDIDVVVLACTHFPLLGDELRAAFPGVALVGGGPGIARR
                     IAWLTREQPWPAEPSGGIALFTGAAPNGALRESLAGFGLEDVRAL"
     gene            7889..8482
                     /gene="plsY"
                     /locus_tag="VFU20_00830"
     CDS             7889..8482
                     /gene="plsY"
                     /locus_tag="VFU20_00830"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEU5285036.1"
                     /translation="MTADLLLAWLLGYLLGSIPFGLLLTRLAGKGDVRDVGSGNIGAT
                     NVLRAGGRGLAAATLLLDALKGAAAVLLARRLFGPGSEMFAATGALLGHLYPAWLRFK
                     GGKGVATLLGILVPLLPIAALIYALVWLALLAAVRISSVAGIAAALSAPVSAAVLGPD
                     YLFPMLLGFALLVLWKHRVNIARLRAGTEPRIGKQRA"
     gene            8479..>9343
                     /gene="dprA"
                     /locus_tag="VFU20_00835"
     CDS             8479..>9343
                     /gene="dprA"
                     /locus_tag="VFU20_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU5285037.1"
                     /translation="MSGDLIDRLRLVRSPGIGPITFRQLIARFGDAAAALAAVPDLAR
                     RGGGRAPSLFGRADAEREIERVESLGARYVTLGQGLYPRLLAEMDDAPPLLMAKGQLS
                     LLERTAVAIVGARNASAAACRFARGLAHGLGQQGAVVVSGLARGIDSAAHDGALDSGT
                     IGVVAGGIDIFYPPENKERQQALFERGLVLAEMPPGTEPRARHFPYRNRIIAGLGLGT
                     VVVEAAPRSGSLITARIAAEAGREVMAVPGSPLDPRAQGCNLLIREGATLVQNADDVL
                     EAIRPLHLGPLR"
BASE COUNT         1365 a         3177 c         3340 g         1461 t
ORIGIN      
        1 ggtcgacgga tgacgaacac cgcttgtttg cctatctccg cgcgaatggc ccgcactccc
       61 gccggaaccc cgccgcatcc caagggcgcc gagcgcttcc acgaggagcg cgcgacttat
      121 gccttgaagg gatcggcgca gcccgatctc gaggccgggg tggcggcgat ccgcgaggtc
      181 gtgcgcacct tgaagcccaa gccgggcgtc taccggatgc acgacgccaa gggcgaggtg
      241 ctgtacgtcg gcaaggcgcg cgcgctgaaa tctcgcgtca ccaactatac ccaggtcaag
      301 aacctgtcga agcggctcca gcggatggtg gcgcagacgc ggtcgatgac gatcgtcacc
      361 accaacaacg aagccgaggc gctgctgctc gaggcgcagc tgatcaagcg ctatcgcccg
      421 gcgtacaacg tgctgctgcg cgacgacaaa agcttcccgt tcatcctgct gcgcgaggac
      481 cacccgttcc cacgggtcca gaagcaccgc ggcgcccgca acgtcaaaag gggccattat
      541 tacgggccgt tcgcgagcgc cgggtcggtc actcgaacac ttaacgcgct gcagaagctc
      601 ttccttttaa gaagttgttc cgacagcttt ttcgccaacc gctcgcggcc gtgcctgctc
      661 taccagatcc gccgctgctc ggcgccgtgc gtcggccgca tcggcgaggc ggattatgcg
      721 gcgctggtcg aggacgcgaa ggcgttcctg gccggcaagt cgaccggcgt ccaggcgcgg
      781 ctcggcaagg cgatggccga ggccgccgag gcgcaggatt acgagctggc cgcggtctac
      841 cgcgaccggc tgcgcgctct gacctacatc cagggcagcc agacggtcca cgccgagggg
      901 ctgggcgacg gcgacatctt cgcgctcgcc tgcaaggccg gggcgatgtc catccaggcc
      961 ttcttcatcc gcggcggcca gaactggggc catcgggcgt tcttcccggc gcacaccgcc
     1021 gacgttcccg aggccgaagt gctgtcgagc ttcctcgtcc agttctacga ggacatgccg
     1081 ccgccgcggc ggatcctggt cgaccgcgag ctcccggatc gggagctgct ggaggaagcg
     1141 ctgtgcgagc gctccggcca caaggtgtcg atcgaccagc cccggcgcgg ggggcgcgcg
     1201 aagctgatcg agcaggcgcg gcgcaacgcc gaggaagcgc tcgagcggcg gatggcggag
     1261 accacctcgc aggcgaaagt gcttcgcgag ctcgccgaca cgttcgagct gcccgacgtg
     1321 cccaggcgca tcgaggtcta tgacaacagc cacatcatgg gcaccagccc gaccggggcg
     1381 atgatcgtcg ccgggcccga gggcttccgc aagaacaact atcgcaagtt caacatcaag
     1441 cggcccgaga ccgctccggg cgacgatttc gcgatgatgc gcgaggtgct cgagcggcgc
     1501 ttcgcccggc tcgagaagga ggatcccgac cgggcgagcg gcgaatggcc cgacctgctg
     1561 atcatcgacg gcggcaaggg gcaattgtcg gccgcctgcg agatgatgga gaatgccggg
     1621 gtccacgaca tcccggtgat cgccgtgtcc aaggggcccg accgcaacgc cgggcgcgaa
     1681 accttccatc tgcccggcgg gcgcgagctg accctgccgc ccaattcggc gctcctgttc
     1741 tatttgcagc ggctccgcga cgaggcgcac cgcttcgcca tcggcaccca ccgcgcgaag
     1801 cggtcgaaga gcctgaccac ctcgacgctc gacgatgtcc cgggcatcgg ccccaaccgc
     1861 aagcgggctc tgctgatgca cttcggcacc gcccgggcgg tcaagggcgc cgcgctcgac
     1921 gacctcgagc gggcgcccgg gatcagcaag gcgatggcgc gccagctcta cgattatttc
     1981 catccgcgtg ggtgaggagc ctaaagttcc tccctatcgc aaggcgatgg ggagggggac
     2041 cagcgcagct ggtggagggg tcatcggcac cggcgcctgt tacccctcca ccatcgaatc
     2101 tgcgttcgat ggtccccctt cccagcctgg gctggggagg aactagtgcg catcgatgtt
     2161 ccggcgatcg tctgcgcgct gcgcgcccac ggcgagcatg gcgcggtggt ccggctgctg
     2221 accgccgagc acgggctcca ggcggcctat gtccgcggcg cccgagggcg gcggatgcgg
     2281 ccggtgctga tcgccgggaa cctggtcgcg gcgaccctga gcgcccggac cgacagccag
     2341 ctgccgcagg cgagcgtcga gctcgtccac agccgcgccc cgctgctcgg cgagccgctg
     2401 ccggccgcgg cgatcgaatg ggcgaccgcg ctgaccgcca ccgcgctccc cgagggccag
     2461 ccgtacccgc ggctgtactc ggcgctcgac gcactgctca cggcgatcga ggcggcgccg
     2521 tcggcgagcg gctggggcgc ggcgctggtg cgctacgagc tgctgctgct cgccgagctc
     2581 ggcttcgggc tcgacctgga gacttgcgcg gtgaccggca cgaaggacga cctggtcgcc
     2641 gtcagcccgg gctcgggccg ggcggtcagc gccgcgcaag cggagcccta tgccggcaaa
     2701 ttgctgccgc tgccgcgctt cgtgcgcgag ggcggggcgg cgagctggac ggacatcgcc
     2761 cacggcctcg acctcaccgg ccatttcctg atgcgcgacc tgctgaccca gaaatcggtg
     2821 gtgctcggcg aggcccggga gcggctggtc gaccggctgc ggcgggcggg cggactcgat
     2881 tagatctgcg gttgtttgga gccgcgcgct cgcctagcgt tcgattcgtg accgcgaccg
     2941 acacgatggc gatgctgttc cccaatgtgg cgacgacccg cgacatctcg gtgcgggtcg
     3001 cggtcagcta tctcgccgag cagtcggacc cctcgtccga gcgctggttc tggtcgtacc
     3061 atgtgcggat cgagaacggc tccgaccgcg cggtccagct gctgtcgcgg agctggcgga
     3121 tcatcgacgg ccgcggcacc gtccacgagg tccacggcga gggcgtggtc ggcgagatgc
     3181 cgctgatcgc cccgggcggg agcttcgatt atgtctcggg ctgcccgctc gacaccccgt
     3241 ccgggtcgat gagcggcagc taccggatgg tcgacgagga cggcaatgcg ttcgacatcg
     3301 ccatcccgcg cttcaccctg ttgagttcgg tcaactagag cagcttgtcc tcggccgggg
     3361 gcggttccgc cttgcgcctg ggttcggcgc gcgccggcgg ctgctcgccg accttgccgg
     3421 cgatgtcggc gaagtcgccg ggctcggtga agtccttgta gacgctggcg aagcggatgt
     3481 aggcgacgtg gtcgatggcg ttcaaaccgt ccatcaccgc ctcgccgatc tgcactgcct
     3541 ggacctcgtc gccgcgggtc tcgagctggc gctggatgct cgacaccagc cgctcgatgc
     3601 ggtcgggggc gatatcgcgc ttgcggcagg cgatggcgat cgagcgctcg agcttggcgc
     3661 ggtcgaacgg ctcgcgctcg ccggtcttct tgaccacggt caggtcgcgc agctggaccc
     3721 gctcgaaggt ggtgaagcgc gccccgcacg cctcgcactg gcggcggcgg cggatcgcgg
     3781 cgccgtcctc gctcgggcgg ctgtctttta cctggctgtc ttcatgggcg cagaacgggc
     3841 agcgcaccgc ctaatcctgg tagatcggga aacgggcgca gagctcgcgg acgcggccgt
     3901 tgacctcgcg ctcgaccgcg ccgttgttct cgagaccgtt cgccccgagc ccgtcgagca
     3961 cgtcggcgac catgtcggcg atgtccctga actcggcctc gccgaagccg cgggtggtgc
     4021 cggccggcga gccgacgcga attccgctgg tctgcaccgg cggcagcggg tcgaacggga
     4081 cgccgttctt gttgcaggtg atcccggcgc gctcgaggct ctcgtcggcg tcgcggccgg
     4141 tgagcttgag cggccgcagg tcgaccagcg ccagatgcgt gtcggtgccg ccggcgacga
     4201 ggtccgcgcc gcgctccttc agccgtcccg cgagcatctt ggcattggcg atcaccgcct
     4261 tggcataggt cttgaagccg ggcttcaatg cctcgccgaa cgcgaccgcc ttggcggcga
     4321 tgacgtgcat cagcggcccg ccctggaggc cggggaagac ggccgagttg atcttcttgg
     4381 cgatcgcctc gtcatcggtc agcaccatgc cgccgcgcgg gccgcgcagc gtcttgtggg
     4441 tggtggtggt gacgacatgc gcgtggccga acgggctcgg atgctcgccg gccgcgacca
     4501 gcccggcgaa atgggccatg tcgaccatca ggatcgcccc gacctcgtcg cagatcgccc
     4561 ggaagcgggc gaagtcgaaa tggcgcggat aggccgatcc gccggcgatg atcagcttgg
     4621 gcttgtgctc gcgggccagc cgctcgacct cgtcgaaatc gacccggtgg tcgtcggggc
     4681 ggaccccgta ctggatggcg ttgaaccatt tgcccgactg ggcgggcgga gctccgtggg
     4741 tcaggtgacc gcccgccgcg aggctcattc cgaggatcgt gtcgccgggc ttcaacagcg
     4801 ccagcatcac cgccccgttg gcctgcgcgc ccgagtgcgg ctggacgttg gcgaagccgc
     4861 agccgaacaa ttgcttcgcc cgctcgatgg ccagcgtctc gaccgcgtcg gacggctcgc
     4921 agccctggta atagcggcgg cccggatagc cctcggcata tttgttggtc agcaccgagc
     4981 cctgtgcctc gagcaccgcc cggctgacga tgttctccga cgcgatcagc tcgatctggg
     5041 tctgctcgcg gcttagctcg tcgcggaccg cgtcggcgac cgccgggtcg gcgtcggcga
     5101 ggcggcgggt gaagaagcct tcgggctgaa cctcgttcaa cttggcggcg ctggccatca
     5161 gtcgctgtcc tgcaaaaggg aagagagctg gtcgacccgc cgctggtggc ggccgccggc
     5221 gaagtcggtg ccgaggaagg cggcgacgca ggccttggcc atgtcgctgc cgatcagccg
     5281 cccgcccaag gccagcacgt tggcgtcgtt atgctcgcgg gcgagcgccg cggagaccgg
     5341 ctcgtcgacc cgcgcgcagc ggcacttggg gttgcggttg acggcgatcg agatgccgat
     5401 gcccgagccg cagacggcga tcccgcgctc ggccgcgccg gtcgcgacgg cattggcgag
     5461 cctggctccg aattccggat aatcgacgct ttcggtgtcg ttggtgccaa ggtcggcgac
     5521 ctcgtagccg agctcgctca gccaggcggc cagctcccgt ttaagggcaa agccggcatg
     5581 gtcggaagca agggcgatgc gcatggccaa tgcctaggcc aagccccgcg gattcgccag
     5641 tggcagcagc gcctcaggcg gcgagcttga gctccagccg gtcccagatc tcgaccagcg
     5701 ccgcggtcag ctcgtccatc atcgcggcgg tgtggcaggg gccgggcgtg aagcgcagcc
     5761 gctcggtgcc gcgcgggacg gtcgggtaat tgatcggctg gacgtagagc ccgtactcgg
     5821 cgagcaatat gtcgctgatc cgcttggcct tgaccgggca gccgaccagc agcgggacga
     5881 tgtgcgtgac gctcggcatc agcggcaggg ccgcgtcccg gaacctcgcc ttgagctgag
     5941 ccgcggcttg gtgctgggcc tcgcgctcga cggacgactg cttgaggtgc ctaacgctcg
     6001 ccagcgcccc ggcgaccagc accggcgaca gcgaggtggt gaagatgaag cccggcgcat
     6061 agctgcggat gcagtcgacg atgttgcggt cggcggcgat gtagccgccc atcaccccga
     6121 acgccttgcc gagcgtgccc tcgatcaggg tgattcggtc ggcgaccgcg tcgcgctccg
     6181 agatgccgcc gccatgctcg ccgtacatcc cgaccgcatg gacctcgtcg agataggtca
     6241 gcgctccgta gcgctcggcg aggtcgcaga tgctggcgat cggggcgatg tcgccgtcca
     6301 tcgaatagac gctctcgaac gcgatcagct tgggcgcgtc ggaatcggct tcgctcagca
     6361 gctgctcgag gtgcgcaagg tcgttgtggc ggaacacgcg tttctcgcag cccgagttct
     6421 tgatcccggc gatcatcgag gcgtggttga gttcgtcgga gaagatcacg cagccgggga
     6481 gcaatttgcc gagggtcgag agcgccgcct cgttcgacac gtagcccgag gtgaacagta
     6541 gcgccgcctc cttgccgtgg agcgccgcga gctcggcctc gaggtcgaca tggtagtggg
     6601 tgttgccgcc gatgttgcgg gtgccgccgg aaccggcgcc gacgtcgtgg agcgcggtct
     6661 ccatcgcctc gaccaccgcc gggtgctgcc ccatcccgag atagtcgttc gagcaccaga
     6721 cgatgatcgg cttggggccg ttgtggccgg cgaagcagcg cgcatcgggg aacgagccct
     6781 tggtcctgag gatgtcgatg aacacgcggt agcggccttc gtcgtgaagg cggtcgatcg
     6841 cagactggaa gatgcggtcg tagtccaacg gtttcggcgc ttccctggct ctcgccgcgc
     6901 cctttagctt tggaggtcgt tcatttccat tgcgaacgac tcgcaacagc tcacagtgcg
     6961 cggacgtcct cgaggccaaa tccggccagc gattcgcgaa gcgccccgtt cggagcagcg
     7021 ccggtgaaca atgcgattcc gcccgaaggc tctgcgggcc acggctgttc gcgcgtcagc
     7081 caggcgatgc ggcgggcgat cccggggccg ccgccgacca gcgcgacgcc cgggaaggcc
     7141 gcccgcagct cgtcgccgag cagggggaaa tgggtgcagg cgaggacgac gacgtcgatg
     7201 tcctggccgt gcccggcgtc gaacatcggc tgcacggcgg cccggaccgc atcgagcgcg
     7261 acctcgtcgc cggcaagctt ggcctcggcc agctcgacca gctcgggcga gccgtggcgg
     7321 atcaccctgc agtcggaagc gaagcgggcg gcgaggtcgt cgacgtaggg ctggcggacg
     7381 gtcgcctggg tgccgagcac gccgatcacc cgggtcttcg acatttcgga cgccggcttg
     7441 atcgccggca ccgtgccgac caccggcagc tcgagcgccg agcggaccgt gtccagcgcg
     7501 atggtcgagg cggtgttgca ggcgatcacc gcgagtcggg ggcggaaccg ctcgaccacc
     7561 cgcccgagca gcgccgggat gcgcgacgcg atgtcgcttt cgctgcgctc gccgtagggg
     7621 aagccggcgt tgtcggcgac gtagacgaag ggagcgctcg gcagcagcgc gcgcgtcggt
     7681 gtgagcaccg acaggccgcc gaccccggaa tcgaagaaga ggatgggagc ggctggattc
     7741 attgcgcgcc tttaacgccg ttcgtcccga gcttgtcgag ggactgactt tctttctaca
     7801 cggggagaag ggcagggctt cgacttcgct cagcccgaac ggaatgggaa aagtcgttgc
     7861 aatgttctga tcttcccgag tgccgtcgat gaccgccgac ctgctccttg cctggctgct
     7921 cggctacctg ctcgggtcga tcccgttcgg gctgctgctg acgcggctcg cgggcaaggg
     7981 cgacgtgcgc gacgtcggct cgggcaatat cggcgcgacc aacgtccttc gcgccggcgg
     8041 cagggggctg gccgccgcca ccctgctgct cgacgcgctc aagggcgccg ccgcagtgct
     8101 gctcgcccgc cgactgttcg gcccgggcag cgagatgttc gccgccacgg gagcgctgct
     8161 cggccatctt tacccggcct ggctgcgctt caagggcggc aagggagtcg cgaccctgct
     8221 tggaatattg gtcccgctgc tgccgattgc cgcattgatc tacgcgctgg tgtggctcgc
     8281 acttctggcc gcggttcgaa tctcgtcggt cgccggaatc gccgccgcgc tcagcgcgcc
     8341 ggtcagcgcg gcggtgctcg gccccgacta tctgtttccg atgctgctcg gtttcgcatt
     8401 gctcgtgctg tggaagcacc gagtgaatat cgcccggctg agggccggca ccgagccccg
     8461 catcggaaag caaagggctt gagcggcgac ctgatcgatc gcctgcggct ggtccgctcg
     8521 ccgggcatcg gcccgatcac cttccggcag ctgatcgccc gcttcggcga tgccgcggcg
     8581 gcgctggccg cggtcccgga cctggcaagg cgcggcggcg ggcgggcgcc gagcctgttc
     8641 ggccgcgccg acgccgagcg cgagatcgag cgggtcgaga gcctcggggc gcgctacgtg
     8701 accctggggc aggggctcta ccctcggctg ctggccgaga tggacgatgc gccgccgctg
     8761 ctgatggcca aggggcagct gagcctgctc gagcgcaccg cggtcgcgat cgtcggcgcg
     8821 cgcaacgcgt cggccgcggc gtgccgcttc gcccgcggcc tcgcccacgg tctcgggcag
     8881 cagggggcgg tggtcgtgtc cggcctcgcc cgcggcatcg acagcgccgc ccacgacgga
     8941 gcgctcgaca gcggcaccat cggcgtggtc gccggcggaa tcgacatttt ctacccgccc
     9001 gagaacaagg agcggcagca agcgctgttc gagcgcgggc tggtgctcgc cgaaatgccg
     9061 ccgggcacag agccgcgggc gcgccatttc ccgtaccgca accggatcat cgccgggctc
     9121 ggcctcggca cggtggtggt cgaggcggcg ccgcgctcgg gctcgctgat caccgcccgg
     9181 atcgccgccg aggcggggcg cgaggtgatg gcggtccccg gctcgccgct cgatccgcgc
     9241 gcgcagggct gcaacctgct gatccgcgaa ggtgcgactt tggtgcagaa cgccgacgac
     9301 gtgctcgagg cgatccggcc gctccacctc gggccgctgc gcg
//
LOCUS       DASTZO010000034         6177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1177849,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000034 DASTZO010000000
VERSION     DASTZO010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6177
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177849"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFU20_00840"
     CDS             complement(<1..400)
                     /locus_tag="VFU20_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285038.1"
                     /translation="MTATPVLAAALAAAPAAARNPATDNPAWTYVQARAAAMSGEHRR
                     SAELLAALAEMNANDATINRKALAEAISAGNMELALRLARRLPQARLPVDARLLLIAE
                     ELKRGRSDLAIGYLSGPLDEGSLSFFAPLLT"
     gene            complement(417..2048)
                     /locus_tag="VFU20_00845"
     CDS             complement(417..2048)
                     /locus_tag="VFU20_00845"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HEU5285039.1"
                     /translation="MQYDVVVVGAGPAGLTAAIRLKQLAAQAGREISVCVLEKGSEVG
                     AHILSGAVIDPKALDELIPDWKAKGAPLTVPVTENHHWVLTRTGKWRMPEWMLPPFMH
                     NDGTYTLSLGNFCRWLAGQAEELGVEIFPGFAAAEILFHDNGSVKGVATGDMGIARDG
                     SRRPDYQPGIELHARYTFFAEGARGHLTKRLTEIFDLRKASGPQVFGIGIKELWDVPA
                     ERHKPGRVIHSQGWPLDDAWGGGFLYHQENSQVALGFVIALDYDNPYLSPFDEFQRWK
                     THPAIRAEIEGGQRVSYGARAINEGGWQAIPELVVPGGALVGCSAGFVNVPRIKGTHT
                     AMKSAMLAAEAAFEAILSDRRGDRLDSYPEALRSSWVDKELRLVRNAQPAVARWGGLL
                     GTLYAGLDMWLGQLGIALPWTLRHHADHTRLKRKEHARPIAYPKPDGVLTFDRLSSVF
                     ISNTNHEEDQPVHLTLKDADIPTAINLPVYAGPEQRYCPASVYEFVESGNGEMRLQIN
                     AQNCVHCKTCDIKDPTQNINWVTPEGGGGPNYPNM"
     gene            2189..2893
                     /locus_tag="VFU20_00850"
     CDS             2189..2893
                     /locus_tag="VFU20_00850"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU5285040.1"
                     /translation="MGGDNRIGSAEAVSALGWWLEAGVDVAVAEQPRNWLERKIEPPN
                     VREPSARAVSQALETLHLFRDWLGTADSLPLGSVGTRRVLPQGHESAPVMLIAEMPGQ
                     EDAAEGRPIGGEAWLLMTRMLAAIGISAEDAYSASLSCFHAPGARMTAGELAECADIA
                     RQHIALARPRRVLLLGDAPARALLGKPLAAARGHVHKIEGVPAVATFHPRFLLNRPSD
                     KALAWRDLLLLMEEEA"
     gene            2890..4668
                     /locus_tag="VFU20_00855"
     CDS             2890..4668
                     /locus_tag="VFU20_00855"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU5285041.1"
                     /translation="MKPRLFAVAALLCASPALAQDPLAPISGPPAPAGQPQPVAAPPA
                     QAVPTPVIATPAPQVAVPPIQVPRDWRGVFAAIRGGDWAAAEAGIALLPGDVLSPVAR
                     AELYTAKGSPVVGLARLEALIAEAPELPQAEQLARMALARGATASPPVVPKRPLTWLG
                     SAPRRSRAQPVQGEPAADQLRAALDPLLKADAAPEAEALLLQAALLLSADARAEAGQR
                     IAWAYYQLGRDQDARRLADMWRIGASGDRAAHSAWISGLASWRQGDCEAASRAFREVA
                     AVSRERELGAGGYYWAARSEQACRRPRSVAPLLKAAARSNESFYGLIALETLGLDTRL
                     PPDPHNHSAAVENLPNVRRALELVNIGERWLAEDMLRHQARIGRPSEHHGLIELAKRL
                     DLAGAQFWLAHNGQPGAVSDPRDRYPVPRWAPITGWRVDPALAFAHIIQESTFRADAV
                     SPADAVGLMQVRPGTAQNMARARNLPYSRASLTDPGYNLEFGQSFIEQMRRSSATGGQ
                     LPRIMAAYNAGPTPVARWAWIPDRGDPLLWIESIPYWETRYYVPAVFRNMWVYQGIAG
                     AEAPTLKSMVEHRWPAFPAGRTHLPQ"
     gene            4859..5929
                     /locus_tag="VFU20_00860"
     CDS             4859..5929
                     /locus_tag="VFU20_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PA0069 family radical SAM protein"
                     /protein_id="HEU5285042.1"
                     /translation="MPVESIRGRGATRNSTPTRFNLKERAIDGDWLDTVEALDGVPKR
                     RTTVTIEHPKSILTRNSSPDIGFDRSINAYRGCEHGCIYCFARPTHAFHDLSPGVDFE
                     SRLFVKPNAAQLLHSALSRPGYECAPIAMGTNTDPYQPIEERWRVTRSVIEVLLETRH
                     PFTITTKSDRVLRDLDLLVPAAELGIVAVAVSVTSLDPAIARTLEPRAPSARKRLEAV
                     RQLNSAGVPCSVAIAPVVPQITDHELEAIVEAAAAAGASGGFYLPVRLPHEVAPLFRA
                     WLDEHYPDRAAKVMATINAMRGGRDNDPNFFSRMRGQGPWAELLRTRFEIAAKKHGLR
                     QAKFELRRDLFRPPEGAQMRLL"
     gene            6000..>6177
                     /locus_tag="VFU20_00865"
     CDS             6000..>6177
                     /locus_tag="VFU20_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M28 family protein"
                     /protein_id="HEU5285043.1"
                     /translation="MTRTLFAALLLAAAAPALSQRVPAQVAALREAALKDDIAFDIVE
                     GLTTEVGPRLAGTEA"
BASE COUNT          872 a         2240 c         2107 g          958 t
ORIGIN      
        1 cggtcaggag cggcgcgaag aacgacaggc tgccttcgtc gagcggcccc gacaaatagc
       61 cgatggcgag gtcggagcgg ccgcgcttca gctcctcggc gatcagcagc agccgggcgt
      121 cgaccggcag cctggcctgc ggcagcctgc gggcgaggcg cagggccagc tccatgttgc
      181 cggcgctgat cgcttcggcg agcgccttgc ggttgatggt cgcgtcgttg gcgttcatct
      241 cggccagcgc cgcgagcagc tcggccgagc gccggtgctc gccgctcatc gccgcggccc
      301 gcgcctggac ataagtccat gccgggttgt cggtggcggg gttgcgcgcc gccgccggcg
      361 cggcggccag cgccgccgcc aacaccggcg ttgccgtcaa tgacctggcg atccatctac
      421 atgttgggat agttgggccc tcctccgccc tcgggtgtca cccagttgat gttctgcgtc
      481 gggtccttga tgtcgcaggt cttgcagtgg acgcagttct gggcgttgat ctggagccgc
      541 atctcgccgt ttccactctc gacaaactcg taaacgctcg ccgggcagta acgctgctcg
      601 ggaccggcat agaccggcag gttgatcgcc gtgggtatgt cggcgtcctt gagcgtcagg
      661 tgaaccggct ggtcctcctc atgattggtg ttggagatga acaccgacga caatcggtcg
      721 aacgtcagca ccccgtcggg cttcggatag gcgattggcc gcgcatgctc cttgcgcttg
      781 aggcgcgtat ggtcggcatg gtgcctcagg gtccacggca aggcgatgcc gagctgaccg
      841 agccacatgt cgagcccggc gtacagcgtg ccgagcagtc cgccccagcg cgccaccgcc
      901 ggctgggcgt tgcggaccag gcgcagctcc ttgtcgaccc agctcgagcg cagcgcctcc
      961 ggataggaat ccagccggtc gccgcggcga tcggacaaaa tggcctcgaa cgccgcctcg
     1021 gcggccagca tcgccgactt catcgccgtg tgggtcccct tgatccgcgg cacgttgacg
     1081 aaccccgccg agcagccgac cagcgccccg ccggggacca ccagctcggg gatcgcctgc
     1141 cagccgccct cgttgatcgc ccgggcgccg taggagacgc gctggccgcc ctcgatctcg
     1201 gcgcggatcg cgggatgggt cttccagcgc tggaactcgt cgaacggcga cagatacggg
     1261 ttgtcgtagt cgagcgcgat cacgaacccc aaggcaacct ggctgttctc ctggtggtag
     1321 aggaagcccc cgccccacgc gtcgtcgagc ggccagccct ggctgtggat cacccgcccg
     1381 ggcttatgcc gttccgcagg aacgtcccac agctccttga tcccgatacc gaaaacttgc
     1441 gggccgctgg ccttgcgcag gtcgaagatc tcggtcagcc gcttggtcag atggccgcgc
     1501 gcgccttccg cgaagaaggt gtagcgggcg tggagctcga tccccggctg atagtcgggg
     1561 cggcgggagc cgtcgcgggc gatgcccatg tcgccggtgg cgacgccctt taccgatccg
     1621 ttgtcgtgga acaggatttc cgccgccgcg aagcccggga agatctcgac ccccagttcc
     1681 tcggcctggc cggcgagcca gcggcagaaa ttgcccaggc tcagagtgta ggtgccgtcg
     1741 ttgtgcatga acggcggcag catccactcg ggcatccgcc acttgcccgt cctggtcagc
     1801 acccagtgat ggttctcggt caccggaacg gtcagcggcg cgcccttggc cttccagtcg
     1861 gggatcagct cgtcgagcgc cttggggtcg atcaccgcgc cggacaggat gtgcgctccg
     1921 acctcgctgc ctttctcgag cacgcagacc gaaatttccc gcccggcttg cgccgccagc
     1981 tgcttgaggc ggatcgccgc ggtcagcccg gcgggcccgg ccccgaccac gacgacgtcg
     2041 tattgcatgg attcgcgttc gatcttccgc tcctctcgcg ccgcgcccat cgcgtctcgt
     2101 cgcgtctcgt cgcggcggca acacagttcg actcgtccgc cctgccaatt ccttgaccgc
     2161 gccgtcaatg gccgcgagga ttccgcacat gggtggggat aatcgcatcg gctcggccga
     2221 ggccgtcagc gccttgggct ggtggctcga ggccggcgtc gacgtcgcgg tcgccgaaca
     2281 gccgcgcaac tggctcgagc gcaagatcga gccgcccaac gtgcgcgagc cgtccgctcg
     2341 ggcggtcagc caggcgctgg agaccctgca cctgttccgc gactggctcg ggacggccga
     2401 ttccctgccg ctcgggagtg tcggcaccag gcgggtcctc ccgcaggggc acgaatcggc
     2461 ccccgtgatg ctgatcgccg agatgccggg gcaggaggat gcggccgaag gacggccgat
     2521 cggcggcgaa gcctggctgc tgatgaccag gatgctcgcc gcaatcggca tctccgccga
     2581 agacgcctac agcgccagcc tgtcctgctt ccacgccccg ggcgccagga tgaccgccgg
     2641 cgagctcgcc gaatgcgccg acatagcccg ccagcatatt gcccttgcca ggccgaggcg
     2701 ggtgctgctg ctgggcgacg ccccggcccg ggcgctgctc ggcaagcccc tcgcggccgc
     2761 gcgcggccat gttcacaaaa tcgaaggcgt tcccgcggta gccacctttc atccgcggtt
     2821 cctgctcaac cggccgtcgg acaaggcgct cgcctggcgg gacctgctgc tgttgatgga
     2881 ggaagaggct tgaagccgcg cctgttcgcc gttgccgccc tgctgtgcgc ttcgccggcg
     2941 ctggcgcagg acccgctggc gccgatttcg ggcccgccgg cgccggccgg gcagccgcag
     3001 cccgtcgcgg ccccgccggc gcaagccgtg ccgacgcccg tcatcgccac gccggcgcct
     3061 caagtcgccg tgccaccgat ccaggtcccg cgcgactggc gcggggtatt cgccgccatc
     3121 cgcggcggcg actgggcggc cgccgaggcc gggatcgcct tgctccctgg tgacgttctg
     3181 tcgccggtcg ccagggccga gctgtacacc gccaaaggct cgccggtggt cggcctcgcc
     3241 cggctcgagg cgctgatcgc cgaggcgccc gagctgccgc aggcagagca gctcgcccgg
     3301 atggcgctgg cccgcggtgc gacggccagc ccgccggtgg tgcccaagcg gccgctgacc
     3361 tggctcggct cggctccccg ccggtcgcgc gcgcaaccgg tccagggcga gcccgccgcc
     3421 gaccagctgc gcgccgcgct cgacccgctg ctcaaggccg acgccgcgcc cgaggccgaa
     3481 gcgctgttgc tccaggccgc gctcttgctg tccgccgacg cccgggccga ggccgggcaa
     3541 cggatcgcct gggcctatta ccagctcggc cgcgaccagg acgcgcgccg cctcgccgat
     3601 atgtggcgga tcggggcgag cggcgaccgg gccgcccatt ccgcatggat ttccggtctg
     3661 gcgtcatggc gtcagggcga ttgcgaggcg gcgtcgcgcg cgttccgcga ggtggcggcg
     3721 gtgtcgcgcg agcgcgagct cggcgcgggc ggctattatt gggcggcgcg gtccgagcag
     3781 gcgtgccggc ggccgcgctc ggtcgctccc ctgctcaagg cggcggcgcg ctccaacgag
     3841 agcttctacg ggctgatcgc gctcgagacg ctgggtctcg acacccgcct gccgcccgac
     3901 ccgcacaacc attcggcggc ggtcgagaac ctccccaacg tccgccgggc gctcgagctg
     3961 gtcaatatcg gcgagcgctg gctggccgag gacatgctgc gccaccaggc gcggatcggc
     4021 cgcccgtccg agcaccacgg actgatcgag ctcgccaagc ggctcgacct ggccggcgct
     4081 cagttctggc tcgcccacaa cggccagccg ggcgcggtgt ccgacccgcg cgaccgctac
     4141 ccggtgccgc gctgggcgcc gatcaccggc tggcgggtcg acccggcgct ggccttcgcc
     4201 cacatcatcc aggaatcgac cttccgcgcc gacgccgtca gcccggccga cgcggtcggc
     4261 ctgatgcagg tgaggcccgg caccgcgcag aacatggccc gggcgcgcaa cctgccctat
     4321 tcgcgggcga gcctcaccga ccccggctac aacctcgagt tcgggcagag cttcatcgag
     4381 cagatgcggc gctcgtcggc gaccggcggc cagctgccgc ggatcatggc cgcctacaat
     4441 gccggcccga ccccggttgc gcgctgggca tggatccccg accgcggcga tcccctgctg
     4501 tggatcgaga gcatccccta ctgggaaacc cgctattatg tgccggcggt gttccggaac
     4561 atgtgggtct accaggggat cgccggggcc gaggcgccga ccctcaagtc gatggtcgag
     4621 caccgctggc ccgccttccc ggccggccgc actcatttgc cccaataagg gccgggaact
     4681 caacgccttc acgctcgctt atgcggtcgt tggaggagcg acctatgtac atcggacttg
     4741 gtggactgat cctgctgatc atcatcctcg tcatcctgtt ctgacgcgga cacagccggg
     4801 ccagcgcgaa gctggcgttt gttcttgata tgttctacaa ccggcctatc ctcgaggaat
     4861 gccggttgag tcgatccgcg ggcgcggagc aacgcgcaac agcactccca cgcgcttcaa
     4921 cctgaaggag cgcgcgatcg acggcgactg gctcgacacg gtcgaggcgc tcgacggcgt
     4981 gccgaagcgg cgcacgaccg tcaccatcga gcacccgaaa tcgatcctga cccgcaacag
     5041 ctcgccggac atcggcttcg accgctcgat caacgcctat cgcggctgcg agcacggctg
     5101 catctactgc ttcgctcggc cgacccacgc gttccacgat ttgtcgccag gcgtggattt
     5161 cgaatcgcgg ctgttcgtca agccgaacgc ggcgcaattg ctccattcgg cgctgtcgcg
     5221 gcccggctac gaatgcgctc cgatcgcgat gggtaccaac accgacccct accagccgat
     5281 cgaggagcgc tggcgggtga cccgctcggt gatcgaggtg ctgctcgaga cccgccatcc
     5341 gttcaccatc acgacaaaat ccgaccgggt gctgcgcgac ctcgacctgc tggtgcccgc
     5401 ggccgagctc gggatcgtcg ccgtcgctgt gtcggtgacc tcgctcgacc cggcgatcgc
     5461 ccggaccctc gagccgcgcg caccgagcgc gcgcaagcgc ctggaggcgg tccgccagct
     5521 gaactcggcc ggagtgccgt gcagcgtcgc catcgctccg gtcgtgccgc agatcaccga
     5581 ccatgagctc gaggcgatcg tcgaggcggc ggccgcggcc ggcgccagcg gcggcttcta
     5641 tttgccggtc cgcctgcccc acgaggtcgc gcccttgttc cgcgcctggc tcgacgagca
     5701 ttacccggac cgagccgcca aggtgatggc gacgatcaac gcgatgcgcg gcgggcgcga
     5761 caacgacccc aatttcttca gccggatgcg ggggcaaggg ccgtgggcgg agctgctccg
     5821 gacccgcttc gagatcgcgg cgaagaaaca cggccttcgc caggcgaagt tcgagctgcg
     5881 ccgcgacctg ttccgcccgc ccgagggagc gcagatgagg ctgctttgat cgcacccgcg
     5941 ggagatttta tcgaaccgcg gggtgactcg cgcggccccg ccgtcctagg cacgcggcca
     6001 tgacccgcac cctgttcgcc gcgctgttgc tcgcggccgc cgcccctgcg ttgtcgcagc
     6061 gcgttccagc gcaggtcgcg gccctgcgcg aggcggcgct caaggacgac atcgccttcg
     6121 acatcgtcga gggcctgacg accgaggtcg ggccgcggct cgcgggcacc gaggccg
//
LOCUS       DASTZO010000035         2068 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_525291,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000035 DASTZO010000000
VERSION     DASTZO010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525291"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VFU20_00870"
     CDS             <1..742
                     /locus_tag="VFU20_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285044.1"
                     /translation="FISVAELSTDRVNAGNANLLPQRAWEFRLTLDRPVLGDGLVKLD
                     LGHDRISLLQDRVLIFDESGQGFDAPGNIGTGKRSFAQLNVDAPLGRLWSGLRVKANA
                     MVQRTRVEDPISGEMRNFSGFYPDWEWYAEARRDSGAFSYGFAISDRDKFTFFRTEEF
                     DSNSNGGPYATAFVEYRPDARTAITLDVDNALDTSASRERLLFFPNRADPEMSIREVR
                     ERNRHLNIGLTVKRSFGGAGVAKTNRGG"
     gene            780..1970
                     /locus_tag="VFU20_00875"
     CDS             780..1970
                     /locus_tag="VFU20_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HEU5285045.1"
                     /translation="MGEPAILPFDWADPFDLDHQLSDEERMVRDTAEGYAQEKLQPRV
                     TDAYLDEHFDRAILSEMGELGLLGATIPNEYGGAGLGYVSYGLIARAVERVDSGYRSA
                     MSVQSSLVMHPIHAYGSEEQRRKYLPGLAKGELVGCFGLTEPDAGSDPGGMRTRAQQV
                     QGGYILTGAKTWITNSPIADIFVVWAKSEAHGGKIRGFVLEKGMKGLSAPAIKQKLSL
                     RASITGEIVMDAVEVPEDALLPDAEGLKGPFGCLNRARYGIGWGSMGAAEACYAAARR
                     YTLDRKQFGRPLAANQLVQLKLANMATEIALGLQAALRVGRRLEAGELIPEAISLIKR
                     NNCGKALDIARTARDMHGGNGISAEFQVMRHAANLETVNTYEGTHDVHALIIGRAITG
                     IPAF"
BASE COUNT          356 a          757 c          653 g          302 t
ORIGIN      
        1 cttcatcagc gtcgccgagc tgtcgaccga ccgggtcaat gccggcaacg ccaatctgct
       61 tccgcaacgc gcgtgggagt tccgcctgac gctcgaccgc ccggtcctcg gcgacgggct
      121 ggtcaagctc gacctcggcc acgaccgaat cagcctgctc caggaccggg tgctgatctt
      181 cgacgagtcc ggccagggct tcgacgcgcc cggcaacatc ggcaccggca agcgcagctt
      241 cgcccagctc aacgtcgacg cgcccctggg ccggctgtgg agcggcctcc gggtcaaggc
      301 caacgccatg gtccagcgca cccgcgtcga ggacccgatc agcggcgaga tgcgcaattt
      361 ctccggcttc taccccgact gggagtggta cgccgaagcc cgccgcgact ccggagcctt
      421 ttcctacggc ttcgcgatca gcgaccgcga caagttcact ttcttccgca ccgaggagtt
      481 cgactccaac tccaacggcg gcccctacgc gacggcgttc gtcgaatatc gcccggacgc
      541 gcgcaccgcg atcacgctgg acgtcgacaa tgccctcgac accagcgcca gccgcgagcg
      601 gctgttgttc ttccccaacc gcgccgatcc cgagatgtcg atccgcgagg tccgcgagcg
      661 caaccgccac ctcaatatcg gcctgaccgt caagcgcagc ttcggcggcg ccggggtggc
      721 gaagacgaac cgaggcggct aaggaacccg cgatttcttg tcatcgcggg gaaacttcag
      781 tgggcgagcc tgcaatcctt ccgttcgact gggccgaccc gttcgacctc gaccaccagc
      841 tcagcgacga agagcggatg gtgcgcgaca ccgccgaggg ctacgcccag gagaagctgc
      901 agccgcgggt caccgacgcc tacctcgacg agcatttcga ccgcgccatc ctctcggaga
      961 tgggcgagct cggcctcctc ggcgcgacca ttcccaatga atatggcggc gccggcctgg
     1021 gctacgtcag ctacggcctg atcgcccgcg ccgtcgagcg cgtcgacagc ggctaccggt
     1081 cggcgatgtc ggtccagtcg agcctcgtca tgcacccgat ccacgcctac ggaagcgagg
     1141 agcagcgccg caaatacctc cccggcctgg cgaaggggga gctggtcggc tgcttcggac
     1201 tcaccgagcc cgacgccggc tccgaccccg gcggaatgcg cacccgcgcc cagcaggtgc
     1261 agggcggcta catcctcacc ggcgcgaaga cctggatcac caactcgccg atcgccgaca
     1321 tcttcgtcgt ctgggccaag tcggaggccc acggcggcaa gatccgcggc ttcgtcctgg
     1381 aaaaaggaat gaagggcctg tcggcgccgg cgatcaagca gaagctcagc ttgcgcgcct
     1441 cgattaccgg cgagatcgtc atggacgcgg tcgaggtccc cgaggacgcg ctgctgcccg
     1501 atgcggaggg gttgaagggc ccgttcggct gcctgaaccg cgcccgctac ggcatcggct
     1561 ggggctcgat gggcgccgcc gaggcctgct acgcggccgc ccgccgctac acgctcgacc
     1621 gcaagcagtt cggccgcccg ctcgccgcca accagctcgt ccagctgaag ctcgccaaca
     1681 tggcgaccga gatcgcgctc gggcttcagg ccgccttgcg cgtcggccgg cggctcgagg
     1741 ccggcgagct gatccccgag gcgatcagcc tgatcaagcg caacaattgc ggcaaggcgc
     1801 tcgacatcgc ccgaaccgcc cgcgacatgc acggcggcaa cggcatttcc gccgagttcc
     1861 aggtgatgcg ccacgccgcc aacctcgaga cggtcaacac ctacgaaggc acccacgacg
     1921 tccacgcctt gatcatcggc cgggcgatca ccggcattcc ggcgttctga tcctttccgt
     1981 catgccgagt gtgggtgaaa accggcgctt tccaacgccg ccgcgtccca gagggcctgg
     2041 ctgggcgaca aatccgatgg cagccgtg
//
LOCUS       DASTZO010000036         4430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1073938,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000036 DASTZO010000000
VERSION     DASTZO010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1073938"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            617..1234
                     /locus_tag="VFU20_00880"
     CDS             617..1234
                     /locus_tag="VFU20_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285046.1"
                     /translation="MPFQKLSYVLAGIGLPALSACTAEQPRPTEPTLAEVKAGAERYR
                     DVKNAIADGYTTDNKCVTAQMLGFPAINGDMGLHYVRRDLLGLPPKPAPGRVSGTGTY
                     TDFTKPAMLVYEPQADGSLELVAVENLVFASEWHKTQQKPPQFRGVNYLLLKDDPATR
                     LDEAHNFEPHYELHLWVFRDNPKGMYAPFNPAVTCRHHKPPAPHS"
     gene            1337..3265
                     /locus_tag="VFU20_00885"
     CDS             1337..3265
                     /locus_tag="VFU20_00885"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEU5285047.1"
                     /translation="MATVFLSYSRDDAPRVRLLASALERAGHIVWWDKHIPGGDEYAK
                     AIEKALDTADAVVVAWSETSVRSAWVRDEAAAGRDSGRLVPVTLDGCLPPLGFRQYQT
                     IDLSSWTGRAGARQLATLKDAVAAKGNRDQPAEAAARPAKVGLPPRRGRWIAAAAAAA
                     LVAAVGLFYPRVGLLAGSGSLAPRVSLAQFELSSGLSPDLSRAMNEEILAAFGAENAV
                     AVTTAGISGGASAPFVLGGSIRKIDDALRFTVNLKDSRSGMVIWSNAFDRAAADPLGP
                     RQVSVAASQVVRCGLWGASSYPRRMPDQALSLYLQFCNELWGGSADEQRIFDAAKRVT
                     AAVPNFSFGWSSLALAAVPLSHRSGSADADTLRKQATEAAQKSIRLDRLNPEGYMAMA
                     GLLPLDRYAEREQLLKKAISVRPTECGCERMAYGDFLTSVGRVEEAVEQYDRARAMMP
                     LAPFTNVRLAQALFVVGRHQDAERILGQMIEVWPDATSLRLLKLKSAYWTGRYDQAIP
                     MLREPGLHLTDGQRDALAVTFEALASNDSAARGRAASALQEMAANPRRNDRLIVAGLA
                     ALDADAAAIQAARNLIHERGPTLADVLFEPNLAEASATPHYAALVRQLGLTGYWQSTR
                     QIPDICRQSRKPIFCTGA"
     gene            3282..3545
                     /locus_tag="VFU20_00890"
     CDS             3282..3545
                     /locus_tag="VFU20_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285048.1"
                     /translation="MRIVIAMLMLGACTAEPQAAREERPAAPAAGQAAPQPQPGQSSM
                     LMSVPEDPEALKKLVAMGYTVHDDHLHAPGVTACPKMGEDPVV"
     gene            3550..>4430
                     /locus_tag="VFU20_00895"
     CDS             3550..>4430
                     /locus_tag="VFU20_00895"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5285049.1"
                     /translation="MRLLLLPLLLAGCTAAPPPKSPVAEAAPVAEAGVAFDRNGELGS
                     FALGLADPKTGRAVTADDPVRVASISKVAVALGVMRLVEQGRLDLDSDVSRWLGWSLR
                     NPAFPDRPISLRMLLSHTSSVRDHDDQYAIPLGGSVRAVMADRTSWDLGHGPGAGYFT
                     YSNMNFPIVASIVELVTRERFDIWMRREILGPMKLDSCFNWPTCSDSTVVRAVVLMQG
                     GKAVRDDHGGKRPDCPVFVRDGEPCDLGRWRLGENGALFSPQGGLRISARGLARIGRM
                     LLNGGTLDGARIVSPRSV"
BASE COUNT          714 a         1554 c         1446 g          716 t
ORIGIN      
        1 cggcgatcag ggcgactgcc gacgacacca tgggcccaag gagcattctg gagtaactca
       61 tacctcacgg tttactccaa aatggttaaa aaactgcacg cgccatgaga acccgaaagc
      121 ggatatagaa tgtgcgatcc agagatgttc taagattgag gaatcatgct gcggagagcc
      181 gtaccctaca cggagctttc gaactgagcc gctccggcaa aagtaacatt taatcaatgc
      241 tttccgttta catgaagcat agcgctcttc cccactgagc gcttgatcat ggagaaggcc
      301 ccgtttgttt acgactacgg ggccttcttt ttcacagccg tccgcgtcgg gtcgaatggc
      361 cttctgcggc gttggcgcag gcattgcgcc ttgcacgttc cctgcctcaa aaaactttta
      421 ccggggccgt gcttgctacg gtccagaact ccgcacacta tctaaatatg gtgccgaacc
      481 aagctggtag gtgggctggg gtcgcttatg aggctgtttt tccatgcgcg ctctattgcc
      541 cttgatgcga gtgctagttc tcgacagaag ttcgttacac agattaatgt ttgcccggcg
      601 taccggagaa cttgcaatgc cctttcaaaa gctcagctac gtcctcgccg gaataggact
      661 gccggcgctg agcgcctgca ccgccgagca gccgcgtccc acagagccga cccttgccga
      721 ggtcaaggcg ggcgccgagc gctaccgcga cgttaagaac gcgatcgctg acggctacac
      781 caccgacaac aagtgcgtga ccgcgcaaat gctcggcttc ccggctatca acggcgacat
      841 gggtctccat tacgtccgcc gcgacctgct cgggctgccg cccaagcccg ctcccggccg
      901 ggtcagtgga accggcacct acaccgactt caccaagccg gcgatgctgg tctacgagcc
      961 gcaggccgac ggctcgctgg agctggtcgc ggtcgagaac ctcgtgttcg cgtcggagtg
     1021 gcacaagacg cagcagaagc cgccgcaatt ccgcggggtc aattacctgc tgctcaagga
     1081 cgacccggcg accaggctcg acgaagcgca caacttcgag ccgcattacg agcttcattt
     1141 gtgggtattc cgcgacaatc ccaagggcat gtacgcgccg ttcaacccgg cggtcacctg
     1201 ccgccaccac aagccgcccg caccgcactc ctgaccccag ccgccggcgt taaccggcgc
     1261 gcttggcaag tcccttgaat cattcttgaa gcggcgggcg gatcgtcgca cactcgccgc
     1321 gctgcagagg ggcaggatgg cgaccgtctt cttgagttac tcgcgcgacg acgcgccccg
     1381 ggtccgcctg ctggcgagcg cgctcgagcg cgccgggcat attgtctggt gggacaagca
     1441 cattcccggc ggcgacgaat atgccaaggc gatcgagaag gccctcgaca ccgccgacgc
     1501 cgtggtcgtc gcgtggagcg agacctcggt gcgctcggcc tgggtgcgcg acgaggcggc
     1561 ggccgggcgc gacagcggcc ggctggtccc ggtgaccctc gacggctgcc tgccgccgct
     1621 cggcttccgc cagtaccaga ccatcgacct gtcctcgtgg accggccgcg ccggcgccag
     1681 gcagctggcg accctgaagg acgcggtggc cgccaagggc aaccgcgatc agccggccga
     1741 agcggcggcc aggccggcca aggtcggtct tccgccgagg cgcgggcgat ggatcgccgc
     1801 cgccgcggcc gccgccctgg tcgccgccgt cggcctattc tatccgcggg tcggcctgct
     1861 cgccggcagc ggctcgctgg ctccccgagt cagcctcgcc cagttcgagc tctcgtccgg
     1921 cctgtcgccg gacttgtcgc gggcgatgaa cgaggaaatc ctcgccgcgt tcggcgccga
     1981 gaacgccgtc gcggtgacca ccgccgggat ctcgggcggc gcatcggccc cgttcgtgct
     2041 cggcgggagc atccgcaaga tcgacgatgc cctgcgcttc accgtcaacc tgaaggattc
     2101 gcgcagcggt atggtcatct ggtcgaacgc gttcgaccgc gcggcggccg atccgctcgg
     2161 gccgcgccag gtctcggtcg cggcgagcca ggtggtccgc tgcgggctgt ggggagcgtc
     2221 ctcctacccg cgccggatgc ccgaccaggc actgtcgctc tacctgcagt tctgcaacga
     2281 gctgtggggc ggatcggcgg acgagcagcg gattttcgac gccgccaagc gggtcaccgc
     2341 cgccgtcccc aatttctcct tcggctggtc atccctggcg ctcgcggcgg tcccgctcag
     2401 ccaccgctcc ggctcggccg acgcggatac gctgcgcaag caagcgaccg aggccgcgca
     2461 gaaatccatc cggctcgatc ggctcaaccc cgaaggctat atggcgatgg cggggctgtt
     2521 gccgctcgac cgctacgcgg agcgcgagca gctgctgaag aaggccatca gcgtccgccc
     2581 gaccgaatgc ggctgcgagc ggatggccta cggcgatttc ctcacctcgg tcggccgggt
     2641 cgaggaggcc gtcgagcaat atgaccgcgc ccgggcgatg atgccgctcg ccccgttcac
     2701 caacgtccgc ctcgcccagg cgctgttcgt cgtcggccgg caccaggatg ccgagcggat
     2761 cctcggccag atgatcgagg tgtggcccga cgcgacctcg ctgcgcctgc tcaagctcaa
     2821 gtccgcttac tggaccgggc ggtacgacca ggcgatcccg atgcttcgcg agcccgggct
     2881 tcacctgacc gacgggcagc gcgacgcgct cgccgtgacc ttcgaggcgc tggcttccaa
     2941 cgactccgcg gcgcgcgggc gagccgcatc ggcgcttcag gaaatggccg ccaacccgcg
     3001 ccgcaacgac cggctgatcg tcgccggcct ggccgcgctc gacgccgacg ccgccgcgat
     3061 ccaggccgcg cgcaacctga tccacgagcg cggcccgacg ctagccgacg tgctgttcga
     3121 gcccaacctc gccgaggcga gcgcgacccc ccactatgcc gcgctggtcc ggcagctggg
     3181 gctgaccggc tattggcaat cgacccgcca gatccccgat atctgccggc agtcccgcaa
     3241 accgatattc tgcacaggcg cctgagcgcg aaggagagga catgcgtata gtcattgcaa
     3301 tgctgatgct cggggcctgc accgccgaac cccaagcggc ccgcgaggag cgcccggcgg
     3361 cgccggcggc cggccaggcc gcgccccagc cgcagccggg ccagtcgtcg atgctgatga
     3421 gcgtcccgga agaccccgaa gcgctgaaga agctggtcgc gatgggctat acggtccacg
     3481 acgaccatct gcatgcgccc ggcgtcaccg cctgcccgaa aatgggcgag gaccccgtcg
     3541 tctagcccaa tgcgcttgct tctcctgccg ctgctgctgg ccggctgcac cgcggctccg
     3601 ccgccaaaat cgccggtcgc cgaggcggcg cccgtcgccg aggccggagt cgcgttcgac
     3661 cgcaacggcg agctcggcag cttcgcgctc ggcctcgccg acccgaaaac cggccgcgcg
     3721 gtcacggccg acgacccggt ccgggtcgcc tcgatcagca aggtcgcggt cgcgctcggg
     3781 gtcatgcggc tggtcgagca gggcaggctc gacctcgaca gcgacgtctc gcgctggctc
     3841 ggctggagct tgcgcaaccc ggcttttccg gaccggccga tcagcctcag gatgctgctg
     3901 tcgcacacca gctcggtccg cgaccatgac gaccaatatg cgattccgct cggcggctcg
     3961 gtccgcgcgg tgatggcgga tcgcacctcg tgggacctcg ggcacggtcc cggcgcgggc
     4021 tatttcactt attcgaacat gaatttcccg atcgtcgcct cgatcgtcga gctcgtgact
     4081 cgcgagcggt tcgatatctg gatgcggcgc gagatcctcg ggccgatgaa gctcgactcc
     4141 tgcttcaact ggccgacgtg cagcgattcg acggtcgtgc gcgcggtggt tctgatgcag
     4201 ggcggcaagg cggtgcgcga cgaccacggc ggcaagcggc ccgactgccc ggtgttcgtg
     4261 cgcgacggcg agccgtgcga ccttggccgc tggcgcctgg gcgagaacgg cgccttgttc
     4321 tcgccgcagg gcgggcttcg gatttccgcc cgcgggctcg cccggatcgg ccggatgctg
     4381 ctcaacggcg ggacgctcga cggagcccgg atcgtctcgc cgcgatcggt
//
LOCUS       DASTZO010000037         3124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_107832,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000037 DASTZO010000000
VERSION     DASTZO010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107832"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1301)
                     /gene="rho"
                     /locus_tag="VFU20_00900"
     CDS             complement(45..1301)
                     /gene="rho"
                     /locus_tag="VFU20_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU5285050.1"
                     /translation="MHLKDLKQKSPAELVTMAEELGVEGASTMRKQDLMFSILKVRAE
                     SGAEIMGMGTIEVLNDGFGFLRSPEANYLAGPDDIYVAPQHVRRFGLRTGDTLEGEIR
                     GPKDGERYFALTRVSQINFDDPEAVRHRVNFDNLTPLYPDSKLKLDSADPTIKDKSAR
                     VIDIVAPQGKGQRALIVSPPRTGKTVLLQNIAKAITDNHPEIFLIVLLIDERPEEVTD
                     MQRSVKGEVISSTFDEPASRHVQVAEMVIEKAKRLVEHKKDVVILLDSITRLGRAYNT
                     VVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIATALIDTGSKMDEVIFEE
                     FKGTGNSEIVLDRKVSDKRIFPSLDVGKSGTRKEELLVDQGTLTKMWVLRRILMQMGT
                     VDAMQFLLDKMKDAKSNEDFFASMNQ"
     gene            complement(1455..1916)
                     /locus_tag="VFU20_00905"
     CDS             complement(1455..1916)
                     /locus_tag="VFU20_00905"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5285051.1"
                     /translation="MPHPSTLPLALVLALASTAACSERQPSSEAASANEQSAPAPRID
                     AQRAQALLAAGGILVDVREPAELAETGKLRSALHLPLPSIRAQASKQRIPPELDAYRA
                     RPVILYCRTGRRSGQAAEILRRLGVTDVYNLGGFEEAARAGFPVEPGAAAD"
     gene            complement(1928..2395)
                     /locus_tag="VFU20_00910"
     CDS             complement(1928..2395)
                     /locus_tag="VFU20_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HEU5285052.1"
                     /translation="MTDLTGLQLTGFLGAAYPWVKAAHVTFVIFWMAGLLLVPRFYVY
                     HHATTPGSAEDRAWIEREDRARTIILTPAMLIVWALGLMLAFHLDAWAQGWFMAKLAL
                     VVALTGYQGWIGGYGKALAAGKRALEDRTLRVMNEVPGIAAAVIVVLVIVRPF"
     gene            complement(2392..>3124)
                     /locus_tag="VFU20_00915"
     CDS             complement(2392..>3124)
                     /locus_tag="VFU20_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HEU5285053.1"
                     /translation="PLAGAELDELSPYPTRLEPIYETVAKVRARLAPGKALIGFAGAP
                     WTVATYMVAGQGSRDQSEARRLAYGDPGRFGAIVERIGEATLEYLSGQVEAGAEAVQL
                     FDSWAGSLAPAEFERWVIAPTARLVERLKARHPGIPVIGFAKGAGGKLGAYARETGVD
                     AVGLDETVDPAWAHRELPEGLPVQGNLDPLALLAGGEAMRCAARRILDALAGRPHIFN
                     LGHGILQETPVAHVDELMAIVRGGE"
BASE COUNT          485 a         1044 c         1089 g          506 t
ORIGIN      
        1 ctcgagaagc tgaagcccga gatcatccgc ccggtcgccg cgccttactg gttcatcgaa
       61 gcgaagaagt cctcgttcga cttggcatcc ttcatcttgt cgagaaggaa ctgcatcgcg
      121 tcgacggtgc ccatctgcat gaggatccgg cgaagcaccc acatcttggt gagcgtgccc
      181 tgatcgacca gcaattcctc cttgcgggtg ccggacttgc cgacgtccag cgacgggaag
      241 atgcgcttgt cgctgacctt gcggtcgagg acgatttcgc tgttgccggt gcccttgaac
      301 tcctcgaaga tcacttcgtc catcttcgac ccggtgtcga tcaaggcggt ggcgatgatc
      361 gacagcgagc cgccttcctc gatgtttcgg gcggcgccga aaaagcgctt cggccgctgc
      421 agcgcgttgg cgtcgacgcc gccggtcagc accttgcccg acgacggcac gacggtgttg
      481 taggcacggc ccaggcgggt gatcgaatcg agcaggatga cgacgtcctt cttgtgctcg
      541 accagcctct tggccttctc gatcaccatc tcggcgacct ggacgtggcg gctggcgggc
      601 tcgtcgaagg tcgaggagat gacctcgccc ttgaccgagc gctgcatgtc ggtgacctcc
      661 tcgggccgct cgtcgatcag cagcacgatc aggaaaatct cgggatggtt gtcggtgatc
      721 gccttggcga tgttctgcag gagcaccgtc ttgccggtcc gcggcggcga gacgatcagc
      781 gcgcgctggc ccttgccctg cggggcgacg atgtcgatca cccgcgccga cttgtccttg
      841 atggtcgggt cggcgctgtc cagcttgagc ttggagtcgg gatagagcgg cgtcagattg
      901 tcgaaattga cccggtggcg gaccgcctcg gggtcgtcga agttgatctg gctgacccgg
      961 gtcagggcga aatagcgctc gccgtccttg gggccgcgga tctcgccctc gagcgtgtcg
     1021 ccggtgcgca ggccgaagcg gcggacgtgc tgcggggcga cgtagatgtc gtcggggccg
     1081 gcgaggtaat tggcctcggg gctgcgcagg aagccgaagc cgtcgttcag cacctcgatg
     1141 gtacccatgc ccatgatctc ggcgccgctt tcggcgcgga ccttgaggat cgagaacatc
     1201 agatcctgct tgcgcatggt cgacgcgccc tcgaccccga gctcctcggc catggtgacc
     1261 agctcggcgg gcgatttctg cttgaggtcc ttgaggtgca tcgggaatct tttcttgtgg
     1321 aagctggagg ttggcgctct ggatcaagcg gatgcggcag gacgcctcat catctgggga
     1381 acgggcaccg gcgagaagag gaaacaggcc gatgaagccg cgtgagtcgg aactatggtc
     1441 gcgagggccg cgtgtcaatc agcggcggcg ccgggctcga cgggaaagcc ggcgcgggcc
     1501 gcctcttcaa agccgccaag gttgtagacg tcggtcactc cgagccggcg gagaatctcg
     1561 gcggcctgcc ccgaacggcg gccggtgcgg cagtagagga tgaccggccg ggcccggtag
     1621 gcgtcgagct ccggagggat ccgctgcttc gacgcctgcg cccggatact cggcaacggc
     1681 aggtgcaggg ccgaccggag cttgccggtc tcggccagct cggccggctc gcgcacgtcg
     1741 accaatatgc cgccggccgc gagaagggcc tgggcccgct gcgcgtcgat ccgcggggcc
     1801 ggagcggatt gttcgttcgc ggacgccgcc tcgctcgaag gctggcgttc cgaacaagcc
     1861 gccgtcgacg cgagcgctag caccaaggcg aggggcaagg tggaaggatg tggcataacc
     1921 ggagcgccta gaacggccgg acgatcacga gcacgacgat caccgccgcg gcgatcccgg
     1981 ggacctcgtt catgacccgc agggtgcggt cctctagcgc gcgcttgccg gcggcgagcg
     2041 ccttgccgta gccgccgatc cacccctgat agccggtcag cgcgacgacg agcgccagct
     2101 tggccatgaa ccagccctgc gcccaggcgt cgaggtggaa ggcgagcatc agccccagcg
     2161 cccagacgat cagcatcgcc ggggtcagga tgatggtccg ggcgcggtcc tcgcgctcga
     2221 tccacgcgcg gtcctcggcc gagccgggag tggtcgcatg gtgatagacg tagaagcgcg
     2281 gcaccagcag cagcccggcc atccagaaaa tgacgaaggt gacgtgcgcg gccttcaccc
     2341 acgggtaagc ggcgccgaga aagccggtca gctgcagtcc ggtcaggtcg gtcattcgcc
     2401 ccctcttacg atcgccatca gctcgtcgac gtgcgcgacc ggcgtttcct gaaggattcc
     2461 gtggccgagg ttgaagatgt gcgggcgccc ggccagcgcg tcaaggattc gccgggccgc
     2521 gcatcgcatc gcctcgccgc cggcgagcag cgccagcggg tcgagattgc cctggaccgg
     2581 caggccctcg ggcagctcgc ggtgcgccca ggccgggtcg accgtctcgt cgagcccgac
     2641 cgcgtcgacc ccggtctcgc gcgcataggc cccgagcttg ccgccggcgc ccttggcgaa
     2701 gccgatcacc gggatgcccg gatggcgcgc cttcagccgc tcgaccaggc gcgcggtggg
     2761 ggcgatcacc cagcgctcga actcggccgg ggcgaggctc ccggcccagc tgtcgaacag
     2821 ctgcaccgcc tcggccccgg cctcgacctg accgctcaga tattcgaggg tcgcctcgcc
     2881 gatgcgctcg acgatcgccc cgaaccggcc cgggtcgcca taggccagcc gccgcgcctc
     2941 ggactggtcg cggctgccct ggccggcgac catgtaggtg gcgacggtcc acggcgcccc
     3001 ggcaaagccg atcagcgcct ttcccggcgc cagcctcgcc cgaaccttgg ccaccgtctc
     3061 gtagatcggc tcgagccgtg tcgggtacgg gctcagctcg tcgagctcgg ccccagccag
     3121 cggc
//
LOCUS       DASTZO010000038         3165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_499500,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000038 DASTZO010000000
VERSION     DASTZO010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499500"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1896
                     /locus_tag="VFU20_00920"
     CDS             <1..1896
                     /locus_tag="VFU20_00920"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5285054.1"
                     /translation="DAAAFGARESVDAVDLSPDGSKVLYLTPGPSRNTFAVIGDLSTG
                     QFQKVVGSDGEPESLRWCNFASMKRMVCRFTGLAESTGIDLLSFSRLIALDADGSNPK
                     LLGQVGSFYDEHVRIYDGRIIDWLIDSDGVVLMNRLYMPEETKIQSLVKQTKKGFGVD
                     KIDVTTLRRQPVEGPREAADYVTDGRGSVRLMSILDTRHSGMLTGELKYSYRTKSSRD
                     WKPLHTVRNIQDPDFVPLAIDADSDSLYVLKKRGGRYALHALRLDGSMAETLVAEHPR
                     VDIDDVVRFGDGQRVIGYTYADEKRATVYFDPEFKMLAASLSKVLPNLPLVYFVDSSR
                     DGRKLLIFAGSDTDPGRYYIFDRDKKTLTEGMHARPELSGRTLAAVKAVTIPASDGAQ
                     IPAYLTLPPGKDPKGLPAIVLPHGGPSARDEWGFDWLPQFLAGRGYAVLQPNYRGSAG
                     YGDSWLKENGFRSWRTSIGDISASARWLSSQGIADPQKLAIVGWSYGGYAALQSAATD
                     PALYKAVVAVAPVTDLALLKEDTRNTVVEELVNRFVGSGPHVAEGSPLRNPGAINAPV
                     LLVHGDKDSNVRVWHSQKMHDALRGAGRQSELLKFKDLDHQLEDPAARTQMLTRIGDL
                     LQRTIGR"
     gene            complement(1952..2566)
                     /gene="rpsD"
                     /locus_tag="VFU20_00925"
     CDS             complement(1952..2566)
                     /gene="rpsD"
                     /locus_tag="VFU20_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU5285055.1"
                     /translation="MSKRSSAKYKLDRRMGENVFGRPKSPVNRREYGPGQHGQRRKGK
                     LSDFGIQLRAKQKLKGYYGDITEKQFKQTYQQASRMKGDASQNLIGLLERRLDMVVYR
                     AKFAPTIWAARQLVSHGHIRVNGTKCNIASRRVDVGDVIELGPKAQEMALTIEAQSLA
                     ERDVPDYVVADGTAKVTYTRVPKLDEVPYPVRMEPNLVIEFYSR"
     gene            complement(2720..>3165)
                     /locus_tag="VFU20_00930"
     CDS             complement(2720..>3165)
                     /locus_tag="VFU20_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285056.1"
                     /translation="HPNATDRMFPNLAVNGLRIDGDTLYVLVANQGKARTRAPIRLIA
                     LAESNGARAEAAPVRIASLPAGQSRWVPVRDFAVKSAAAGRSPAVALEDASFVSASVR
                     LSPAIPAAVDRTGQACLPSRGCVLELDEANNSVGVDGRSIPRGRP"
BASE COUNT          508 a         1027 c         1092 g          538 t
ORIGIN      
        1 gatgccgccg cgttcggggc gcgggagtcg gtcgatgccg tcgatctctc gcccgacggg
       61 tccaaggtcc tgtacctgac gccgggcccg agccgcaaca ccttcgctgt gatcggcgac
      121 ctttcgaccg ggcaattcca gaaagtggtc ggttccgacg gcgagccgga atcgctgcgc
      181 tggtgcaatt tcgcgtcgat gaagcggatg gtctgcaggt tcaccgggct ggccgagagc
      241 accggcatcg acctgctcag cttcagccgg ctgatcgcgc tcgatgcgga cggcagcaat
      301 cccaagctcc tcggccaggt gggcagcttc tacgacgagc acgtgaggat ctacgacggc
      361 cgaatcatcg actggctgat cgattccgac ggcgtggtcc tgatgaaccg cctctacatg
      421 ccagaggaga cgaagatcca gtcgctggtc aagcagacca agaagggctt cggggtcgac
      481 aagatcgacg tgacgacgct ccggcgccag cccgtcgagg ggccgcgcga ggcggccgac
      541 tacgtgaccg acgggcgcgg atcggtgcgg ctgatgtcga tcctcgacac ccggcacagc
      601 ggaatgctga ccggcgagtt gaaatattcc taccggacca aaagctcccg cgactggaag
      661 ccgctgcaca cggtcaggaa catccaggac cccgatttcg tgccgctagc gatcgatgcc
      721 gacagcgatt cgctttacgt cctgaagaaa aggggcggcc gatatgcgct tcacgcgctc
      781 aggctcgacg ggagcatggc ggaaacgctc gttgccgagc acccgcgcgt cgacatcgac
      841 gacgtggtgc ggttcggcga cgggcagcgg gtgatcggct acacctacgc cgacgagaag
      901 cgcgcgacgg tttatttcga cccggagttc aagatgctgg cggcgtcgct ctcgaaggtg
      961 ctgccaaacc tgccgctggt ctatttcgtc gattccagcc gtgatggccg caagctgctg
     1021 atcttcgcgg gcagcgacac ggatccgggc cgctactaca tcttcgaccg cgacaagaag
     1081 accctgaccg agggcatgca tgcccggccg gagctcagcg gccggaccct cgccgcggtc
     1141 aaggcggtga cgatccccgc ttccgatggg gcgcagattc cggcgtacct gacgcttccg
     1201 cccggcaagg acccgaaggg ccttccggcg atcgtgcttc cccacggcgg ccccagcgcg
     1261 cgcgatgaat ggggcttcga ctggctgccg caattcctcg ccggccgcgg ctatgccgtg
     1321 ctgcagccca attaccgcgg ctccgccggc tatggcgatt cctggctcaa ggaaaatggg
     1381 ttcaggagct ggcgcacctc gatcggcgac atttcggcga gcgcacgctg gctgtcttcg
     1441 caaggaatcg ccgacccgca gaagctggcg atcgtcggct ggtcgtacgg cggctatgcg
     1501 gcgctgcagt cggcggcgac cgacccggcg ctgtacaagg cggtggtggc agtcgcgccg
     1561 gtcaccgacc tggcgctgct caaggaggat acgcgcaaca ccgtcgtcga ggagctggtg
     1621 aatcgctttg tcggttccgg cccgcatgtc gccgagggct cgccgctgcg caaccccggg
     1681 gcgatcaacg ccccggtgct gctcgttcac ggagacaagg attcgaacgt ccgtgtctgg
     1741 cactcgcaga agatgcacga cgcgctgcgc ggcgccggca ggcaaagcga gctgctgaag
     1801 ttcaaggacc tcgaccacca gctcgaggat ccggccgcgc gcacgcagat gctgacccgg
     1861 atcggcgacc tgctgcagcg gaccatcggc cgctaaacaa gaagggcggc ccgcgaaggc
     1921 cgccttcgtt ggtcggttta tcgagcggtg gtcagcgcga gtagaactcg atgaccaggt
     1981 tcggctccat ccgcaccgga tagggcacct cgtcgagctt gggcacccgg gtgtaggtca
     2041 ccttggcggt gccgtcggcg acgacgtaat cgggcacgtc gcgctcggcg aggctctgcg
     2101 cctcgatggt cagcgccatc tcctgcgcct tggggccgag ctcgatgacg tcgccgacgt
     2161 caacccggcg cgaggcgatg ttgcacttgg tgccgttgac ccggatgtgg ccgtggctga
     2221 ccagctggcg ggcggcccag atggtcggcg cgaacttggc gcggtagacg accatgtcga
     2281 gccggcgctc gagcagcccg atcaggttct gcgacgcgtc gcccttcatc cggctggcct
     2341 gctgataggt ctgcttgaac tgcttttcgg tgatgtcgcc gtaatagccc ttgagcttct
     2401 gcttggcgcg cagctggatc ccgaaatccg acagcttgcc cttgcggcgc tggccgtgct
     2461 ggcccgggcc gtattcgcgg cggttgaccg ggctcttggg gcgtccgaag acgttctcgc
     2521 ccatccgccg gtcgagcttg tacttggcgc ttgagcgctt cgacatgagg tttccttcaa
     2581 ttgcgttcga cttcgttccc ggggccgcgc tgcttgggct ttggatgccg gcagggccac
     2641 cgcttcaccg ggagtgcggg gccaattgcg agcggcgcgc ctagccgagg gcgcccgaag
     2701 ggtcaagttt tgcggccggt tacgggcgtc cgcggggaat cgagcggccg tcgaccccga
     2761 cgctgttgtt cgcctcgtcg agctcgagca cgcagccgcg gctcgggagg caggcctggc
     2821 cggtgcggtc gaccgcggcc ggaatcgccg gcgacagcct gacactggct gaaacgaagc
     2881 tcgcatcctc gagcgcgaca gccggcgatc gcccggccgc agccgatttc accgcgaaat
     2941 cgcgaaccgg gacccagcgg ctttgcccgg ccggcaggct ggcgattcgg accggagcag
     3001 cctcggccct ggcgccgttc gattcggcca gggcgatcag ccggatcggc gcccgggtcc
     3061 gggctttgcc ctggttggcg accagcacgt agagcgtatc gccgtcgatc cgcaggccgt
     3121 tgacggcgag gttcgggaac atccggtcgg tggcgttggg atggg
//
LOCUS       DASTZO010000039        11077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_838740,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000039 DASTZO010000000
VERSION     DASTZO010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 11077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11077
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838740"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..778)
                     /locus_tag="VFU20_00935"
     CDS             complement(155..778)
                     /locus_tag="VFU20_00935"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008674 -
                     2-dehydro-3-deoxy-6-phosphogalactonate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0034194 - D-galactonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HEU5285057.1"
                     /translation="MSQDIRDYLRHCPLVAITRGIAPDEAEAVGAAIFEAGIRIIEVP
                     LNSPDPLASIKLLSDRLGDRALVGAGTVLDPRQVAEVAAAGGRLVVSPNTDPAVIRAT
                     LAAGLVSCPGYFTPSEAFAALAAGASALKFFPAEAATPAVLRAQKAVLPKDVPVIVVG
                     GVKPETMRPWLAAGADAFGLGSGLYKPGQSAAETADRARAYVAGLSG"
     gene            complement(775..1647)
                     /locus_tag="VFU20_00940"
     CDS             complement(775..1647)
                     /locus_tag="VFU20_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HEU5285058.1"
                     /translation="MAEGFIAVDWGTTNRRAYMIDGGGRCAGEFEDSEGVLSVPAGGF
                     ERAVADIRSRLGDKPLLLAGMIGSNRGWVEAPYVPAPAGLDELARALVWPDERTAIVP
                     GVCDGERADVMRGEEVQLLGAAADGLIPPDCLVCHPGTHNKWVEVAGGRIAAFRTIMT
                     GELFNLLREHSVLADLLAEPAEPGDAFEAGVRRGLASDIQPQLFSVRARVLLGKARRE
                     DAPSYTSGLLIGGDVRSGLDSIAPATRIVVMGRPELTRLYAAALAIAGREPVELDGER
                     CFIAGIRHIAERIE"
     gene            1758..2633
                     /locus_tag="VFU20_00945"
     CDS             1758..2633
                     /locus_tag="VFU20_00945"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEU5285059.1"
                     /translation="MKAGSDVQCVADVHAVLGEGPVWVGREAALYWLDIKGRKIFRLG
                     DGGGLDQWETPLRIGSLAPRASGGFIAGTEHGIAEIDLESGRFAIVHQPEEHLPDNRF
                     NDGKVDRSGRFWAGTMDDTERDSTGTLYRIGRDLGCTAVDGGYRVTNGPAFSPSGEIM
                     YHNDSARQVTYAFDLDRDGNASNRRVFANYGEGDGYPDGMTVDADGCLWIAFWDGWCV
                     RRYSPAGECLETIAVPVARPTSCAFGGPGLDRLYITSASIGLDEAALAAQPQAGGLFI
                     GAPGVRGVADIPFAG"
     gene            2702..3964
                     /locus_tag="VFU20_00950"
     CDS             2702..3964
                     /locus_tag="VFU20_00950"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEU5285060.1"
                     /translation="MFATGIENSIPTINHGKTRVDQMESSGHYERWREDFDCVQEIGI
                     NFLRYGPPIHRTFLGPNKFDWEFSDLTFADLKKREITPIVDLCHFGLPDWMGNFQNPD
                     FPQLFANYAGAFAERYPWAQLYTPINEMFICAVFSAKYGWWNEQKKDDRSFVTALKHI
                     VKANVLAMIEILKRRHDAIFIQSESTEYYHADSPAAIGRAEVLNQMRFLSLDLNYGRR
                     LDSGMYEFLTDNGMTREEYHWFLEHRLKQHCILGNDYYMLNEHRVFADGRTVAAGEVF
                     GYTEITRQYYNRYRVPVMHTETNLWEGPHGDEAVKWLWKEWANVLRLRNVGIPTVGFT
                     WYSLIDQIDWDTALREKNGNVNPLGLFDLDRKIRNVGRAYKQLIADWRGVLPASSVCL
                     VVPIKKLSEHDERDAAGRELDETRLAGE"
     gene            3966..5105
                     /gene="dgoD"
                     /locus_tag="VFU20_00955"
     CDS             3966..5105
                     /gene="dgoD"
                     /locus_tag="VFU20_00955"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HEU5285061.1"
                     /translation="MNIGRIETFFVPPRWLFVRVEAGDGAVGWGEASLEGHAEAVDGA
                     FEALRDRFVGHDARRIEDIWQVAYRGGFYRGGPVLMSALAGLEQALWDLKGRAVGLPV
                     WEMLGGRVRDRIRAYAWIGGDRPNEIADAAKARRDQGFSAVKMNATAELDWIGTPRLF
                     DEVVKRVEAAQAAGMDVGLDFHGRVHRPMAKQLAKALEPLGLLFIEEPLLSENPEGLR
                     QIAELCSTPIALGERLYSRWDFKPFFEMGAVDIIQPDLSHAGGILECRKIAAMAEAYD
                     IAVAPHCPLGPLALASCLQLAACTPNVAIQEMSLGIHYNVGHDLLEFCSDKEVLTPQD
                     GYLAIPDKPGLGITIDEQAVREAHKDRHRWRNPIWRHPDGSFAEW"
     gene            complement(5118..5555)
                     /gene="paaD"
                     /locus_tag="VFU20_00960"
     CDS             complement(5118..5555)
                     /gene="paaD"
                     /locus_tag="VFU20_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HEU5285062.1"
                     /translation="MTTADIRAVLETVMDPEIPVVSVVDLGIVRDVASDRVTITPTYS
                     GCPATEVIERTIREALDAAGFADVRIETELAPPWTTDWISERGKAALKAYGISPPELA
                     KAATCPRCGSTDTEEVSRFGSTPCKAQWRCKACLEPFDRFKCH"
     gene            complement(5552..6754)
                     /gene="pcaF"
                     /locus_tag="VFU20_00965"
     CDS             complement(5552..6754)
                     /gene="pcaF"
                     /locus_tag="VFU20_00965"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HEU5285063.1"
                     /translation="MTEAFICDAVRTPIGRYGGALAPIRADDLAAVPIRALIGRNPGV
                     DWGELDDVILGCANQAGEDNRNLARMAGLLAGLPDSSGGVTLNRLCGSGLDAVAMAAR
                     AIRGGESDLIIAGGSESMSRAPFVLPKASQPFDRTAEIYDTTIGWRFVNPKMRDSYGD
                     DTMPSTAENVAQEFQVTREDQDAFALRSQQRTAAAQASGRLAAEIVPVEIPQRRGDPL
                     VVDRDEHPRATSLESLAKLKPIVRADGTVTAGNASGVNDGAAAIIVASEEAARRNGLT
                     PRARVLGAAVAGVPPRIMGIGPAPATEKLLKRLGLKIEQMDVIELNEAFAAQALAVLR
                     QLGLPDDAAQVNPNGGAIALGHPLGMSGARLALTAAEELAGNGGKLALATMCIGVGQG
                     IALALERV"
     gene            complement(6856..7944)
                     /gene="hppD"
                     /locus_tag="VFU20_00970"
     CDS             complement(6856..7944)
                     /gene="hppD"
                     /locus_tag="VFU20_00970"
                     /EC_number="1.13.11.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003868 - 4-hydroxyphenylpyruvate
                     dioxygenase activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylpyruvate dioxygenase"
                     /protein_id="HEU5285064.1"
                     /translation="MATAVAEKGPLGLENPMGTDGFEFVEYAAPDSELLRSLFTKMGF
                     PEVARHKRKNVTLHRQGDCNFIINAEPDSFAEEFAAIHGPCACAMAFRVKDATAAHAR
                     ALRLGATDVKSDVAGGELEIPAIEGIGGSRLYFVDRYGDKGTIYDIDFDFHPDWRERE
                     AAHASRLTYIDHLTHNVNRGRMSVWADFYEKLFNFREIRYFDIEGKVTGLFSKAMTSP
                     CGKIRIPLNESQDDKSQIEEFLREYKGEGIQHIALGTDDIYAAVDILRARGIPFQDTP
                     GTYYELLDSRIEGHSESVPELQKRGILMDGAPTEGQGLLLQIFTQNVIGPIFFEIIQR
                     KGNEGFGEGNFKALFESIELDQIRRGVI"
     gene            8028..8288
                     /locus_tag="VFU20_00975"
     CDS             8028..8288
                     /locus_tag="VFU20_00975"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEU5285065.1"
                     /translation="MDRPPPDGWTLDESGKVLLRTYRFADFAEAFAFLTRVAEHAERV
                     NHHPEFTSVWNRVDFRLTTHDSGGLTNKDYALAEAIDALAGR"
     gene            complement(8308..8805)
                     /locus_tag="VFU20_00980"
     CDS             complement(8308..8805)
                     /locus_tag="VFU20_00980"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5285066.1"
                     /translation="MRTREPRRKVMLNARMRVAATWNDVCILNISTRGLLVQTSEPPA
                     RGTYLDLHRGQYAIVGRVVWVEGHRFGIRTQDALPVEEIIHPPDPAIAQLAGAVAAEA
                     ALERRAAPHRAQARHDASRMLSRSVEFAVVALFGAAVGVTVFNSVGQALGRPLAQLSA
                     TLSQR"
     gene            9198..9968
                     /locus_tag="VFU20_00985"
     CDS             9198..9968
                     /locus_tag="VFU20_00985"
                     /inference="COORDINATES: protein motif:HMM:NF012571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HEU5285067.1"
                     /translation="MLHKAIATTDRPRGWRDYVVPQCWERFTNDDHAVWDLLFARQVE
                     LLGSRVVAPFLDGIGLLRLSHPGVPDLDELNAILGPRTGWRTVAVTGLVPDDIFFAML
                     SERVFPVGNFIRRREQLDYLEEPDCFHDLFGHIPMLAHHDYAEMIEHVGRLGGAAIAA
                     GQGERVARIYWHSVEFGLAREGGELKILGAGLASSFGEAHFSLESESVERLPFSVPGA
                     VCTAYRHDAFQPLYLVSESLEATVAEILATDAERLLAI"
     gene            10033..>11077
                     /locus_tag="VFU20_00990"
     CDS             10033..>11077
                     /locus_tag="VFU20_00990"
                     /inference="COORDINATES: protein motif:HMM:NF012451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEU5285068.1"
                     /translation="MTPDHYAILGVSPSSEDVEIRTAYLGLMRRYHPDRNSSPEAAER
                     ARAITTAYAVLSDWDRRAEYDLKRARLRSLEATMNVAPRRPPPSPSAMFAVAAVSLLL
                     LVAIRPPLFVRELPERAPAAAPVHKAAAPKPDPAAYCRSPATRDQIRRELFRRAARLR
                     GKDQAAFDRIAGHALIRIDSPELRHADDKRGTVRCEAWVALDLPPGVAVLGGRRNLMA
                     DIGYTVKTARGGRGSVRLSGEGLIVTPLATLAQIARPAEEPPEATAIEPRLAEARPRL
                     DQPPPPKPAPAPPPARMAPQPARAAPQIAKAASQPARATQIAEAAAQPARPTAQAATP
                     QPRPSFSCQIARGR"
BASE COUNT         1712 a         3809 c         3846 g         1710 t
ORIGIN      
        1 tcttcaagcg tcttgaaatg cggcagatcc ggctgcgagg ccttggggtc ggcgacggcg
       61 gccagcgtca cgccatccgt cgccgcgatc gcgggaacgt gctgatcgtg cgcgatcttg
      121 ccgaagccga cgatggcgat cttgagcggg cgcatcagcc gctcagcccg gcgacgtagg
      181 cgcgcgcccg gtcggcggtc tcggcggccg actggcccgg cttgtagaga cccgatccga
      241 gcccaaacgc gtccgcgccc gccgcgagcc acggccgcat cgtctcgggc ttcaccccgc
      301 cgacgacgat caccggcacg tccttcggaa gcaccgcttt ctgcgcccgg agaaccgcgg
      361 gcgtcgcggc ttcggcgggg aagaatttga gcgcggaagc gccggcggcg agcgcggcga
      421 aggcctccga cggagtgaaa tagccggggc acgaaaccag gcccgcggcg agcgtcgctc
      481 ggatcacggc cgggtcggtg ttgggcgaga ccaccagccg cccgccggcg gccgcgacct
      541 cggcgacctg gcgtgggtcg agcaccgtcc cggcgccgac cagcgcgcga tcgccgaggc
      601 ggtccgaaag cagcttgatg ctggccagcg ggtcgggcga gttcagcggc acctcgatga
      661 tccggatccc ggcttcgaag atcgccgctc cgaccgcttc ggcctcgtcg ggcgcgatcc
      721 cgcgggtgat cgcgaccagc gggcagtgcc gcaaataatc ccggatatcc tggctcattc
      781 gatccgctcc gcgatgtgcc ggatgccggc gatgaagcag cgctcgccgt cgagctcgac
      841 cggctcgcgg ccggcgatcg ccagggccgc cgcatagagt cgggtgagct cggggcggcc
      901 catgacgacg atcctcgtcg ccggcgcgat cgagtccagg ccgctgcgga cgtcgccgcc
      961 gatcagcagg ccgctggtgt aggacggagc gtcctcgcgc cgcgccttgc cgagcagcac
     1021 ccgcgcgcgg accgagaaca attgcggctg gatgtcggag gcgagcccgc ggcgcacccc
     1081 ggcctcgaac gcgtcgccgg gctcagccgg ttcggccagc aggtcggcga ggacgctgtg
     1141 ctccctcagg aggttgaaca gctcgccggt catgatggtt cggaaggcgg cgatccgccc
     1201 gcccgcgacc tcgacccatt tgttgtgggt gccaggatgg cagaccaggc agtcgggcgg
     1261 gatcagcccg tcggcggcgg cgccgagcaa ctgcacctcc tcgccgcgca tcacgtcggc
     1321 gcgctcaccg tcgcacactc ccgggacgat cgcggtccgc tcgtcgggcc agaccagcgc
     1381 ccgggcgagc tcgtccagcc cggcgggcgc cggcacgtag ggcgcctcga cccagccgcg
     1441 gttggagccg atcatcccgg cgagcagcag cggcttgtcg ccgagccgcg accggatgtc
     1501 cgcgaccgcc cgctcgaagc cgccggcggg caccgacagc acgccttcgg aatcctcgaa
     1561 ctcgccggcg cagcggcccc cgccgtcgat catataggcg cggcggttgg tcgtccccca
     1621 atcgacggcg atgaaacctt ctgccatccg catgcgtcgg ctcattggac gaaaattcgc
     1681 gacggcgcga cggggtccga aggttttctt ttgtggaggc ggggctagta gcgcgcaaat
     1741 atgaaggcga gccgcggatg aaggccggat cggacgtcca atgcgtcgcc gacgtgcacg
     1801 ccgtgctcgg cgaagggccg gtgtgggtcg ggcgcgaggc ggcgctctac tggctcgaca
     1861 tcaaggggcg gaagatcttc cggctcgggg atggcggcgg gctcgaccag tgggaaacgc
     1921 cgctgcgcat cggctcgctg gcgccgcggg caagcggcgg cttcatcgcc gggaccgagc
     1981 acgggattgc cgagatcgac cttgagtccg ggcggttcgc gatcgtccac caacccgagg
     2041 agcatttgcc cgacaaccgc ttcaacgacg gcaaggtcga ccgctccggc cgcttctggg
     2101 ccgggaccat ggacgacacc gagcgcgatt cgaccgggac gctctaccgg atcgggcggg
     2161 atctaggctg caccgcggtc gacgggggct acagggtcac caacgggccc gcgttcagcc
     2221 cgtcaggcga gatcatgtac cacaacgatt ccgcgcggca ggtgacctac gcgttcgacc
     2281 tcgaccgcga cggcaacgcc agcaaccgcc gggtgttcgc gaactacggc gagggcgacg
     2341 gctatcccga cgggatgacg gtcgatgccg acggctgcct gtggatcgct ttctgggacg
     2401 gctggtgcgt gcggcgctac tcgccggccg gcgagtgcct ggagacgatc gctgtgccgg
     2461 tggctcggcc gaccagctgc gcgttcggcg ggcccggcct cgaccgcctc tacatcacct
     2521 cggcgagcat cggcctcgac gaggccgcgc tggccgcgca accccaggcc gggggtttgt
     2581 ttataggcgc gccgggcgtt aggggcgtcg cggacattcc ctttgcaggc tgagcccgtt
     2641 ttgcagacga actcaggacc gacgggcagc gcgtcaagga gattgagcca tcgctaactt
     2701 catgttcgcg accgggatcg agaacagcat cccgacgatc aatcatggca agacccgcgt
     2761 cgaccagatg gagtcgagcg gccattacga gcggtggcgc gaggatttcg actgcgtcca
     2821 ggaaatcggc atcaacttcc tgcgctacgg gccgccgatc caccgcacct tcctcggccc
     2881 caacaagttc gactgggaat tctccgacct gaccttcgcc gacctgaaaa agcgcgagat
     2941 caccccgatc gtcgacctgt gccacttcgg cctgcccgac tggatgggca atttccagaa
     3001 cccggatttc ccacagctgt tcgccaatta tgccggcgcg ttcgccgagc gctacccgtg
     3061 ggcgcagctc tacacgccga tcaacgagat gttcatctgc gcggtgttct ccgccaaata
     3121 cggctggtgg aacgagcaga agaaggacga ccgctcgttc gtcaccgcct tgaagcacat
     3181 cgtcaaggcc aatgtgctgg cgatgatcga gattttgaag cgccgccacg acgcgatctt
     3241 catccagagc gaatccaccg aatattacca tgccgactca cccgccgcga tcggccgcgc
     3301 cgaagtcctg aaccagatgc ggttcctgtc gctcgacctc aattacggcc gccggctcga
     3361 cagcgggatg tacgagttcc tgaccgacaa cggcatgacc cgcgaggaat atcactggtt
     3421 cctcgagcac cggctcaagc agcactgcat cctcggcaac gattattaca tgctcaacga
     3481 gcaccgcgtg ttcgccgacg ggcgcacggt cgcggccggc gaggtgttcg gctacaccga
     3541 gatcacccgc caatattaca accgctaccg ggtgccggtg atgcacaccg agaccaattt
     3601 gtgggagggt ccgcacggcg acgaggcggt caagtggctg tggaaggaat gggccaacgt
     3661 gctccggctg aggaacgtcg gaatcccgac cgtcggcttc acctggtact cgctgatcga
     3721 ccagatcgac tgggacaccg cgctccgcga gaagaacggc aacgtcaacc cgctcggcct
     3781 gttcgacctc gaccgcaaga tccgcaacgt cggccgcgcc tacaagcagt tgatcgccga
     3841 ctggcggggc gtcctgccgg cctcatccgt ctgcctggtc gtgccgatca agaaactgag
     3901 cgagcacgac gagcgcgacg cggccggccg cgagctcgac gaaacgcgcc tcgccggaga
     3961 atgaaatgaa catcggccgc atcgaaacct tcttcgtgcc gccgcgctgg ctgttcgtgc
     4021 gcgtcgaagc gggcgacggc gcggtcggct ggggcgaggc cagcctcgag ggccatgccg
     4081 aagccgtcga cggcgcgttc gaggcgcttc gcgaccgctt cgtcggccac gacgcgcggc
     4141 gcatcgagga tatctggcag gtcgcctacc gcggcggctt ctaccgcggc gggccggtgc
     4201 tgatgtcggc gctcgccggg ctcgagcagg cgctgtggga cctgaagggc agagcggtcg
     4261 ggctgccggt ctgggagatg ctgggcgggc gggtgcgcga caggatccgc gcctatgcgt
     4321 ggatcggcgg cgaccggccg aacgagatcg ccgacgcggc caaggcccgc cgcgaccagg
     4381 gcttttcggc ggtcaagatg aacgccaccg ccgagctcga ctggatcggc acgcccaggc
     4441 tgttcgacga ggtggtcaag cgcgtcgagg cggcgcaggc ggccgggatg gacgtcgggc
     4501 tcgatttcca cgggcgggtg caccggccga tggccaagca attggccaag gcgctcgagc
     4561 cgttgggcct gctgttcatc gaggagcccc tgctgtccga gaatccggaa gggcttcggc
     4621 agatcgccga gctgtgcagc accccgatcg cgctcggcga gcggctgtat tcgcgctggg
     4681 acttcaagcc gttcttcgag atgggcgcgg tcgacatcat ccagcccgac ctcagccacg
     4741 ccggcggaat cctcgagtgc cgcaagatcg cggcgatggc cgaggcctac gacatcgccg
     4801 tcgcgccgca ctgcccgctg gggcccctgg cgctggcctc gtgcctgcag ctcgccgcct
     4861 gcacgcccaa cgtcgcgatc caggagatga gcctcgggat ccactacaac gtcggccacg
     4921 atttgctgga gttctgcagc gacaaggagg tgctgacccc gcaggacggc tatctcgcga
     4981 tccccgacaa gcccggcctc gggatcacca tcgacgagca ggcggtgcgc gaggcgcaca
     5041 aggaccgcca ccgctggcgc aaccccatct ggcggcaccc ggacggaagc ttcgcggagt
     5101 ggtagccgcc tagcccgtca gtggcatttg aagcggtcga acggctcgag gcacgccttg
     5161 cagcgccatt gcgccttgca cggggtcgag ccgaagcggc tgacttcctc ggtgtcggtc
     5221 gagccgcacc tggggcaggt cgccgccttg gcgagctcgg gcggcgagat cccgtaggcc
     5281 ttcagcgccg ctttgcccct ctcgctgatc cagtcggtgg tccacggcgg agcgagctcg
     5341 gtttcgatcc tgacgtcggc gaagccggcc gcatcgagcg cttcgcggat ggtgcgctcg
     5401 atcacctcgg tcgccgggca gccggaatag gtcggggtga tggtgacgcg atcggaggcg
     5461 acgtcgcgga cgatgccgag gtcgaccacc gagaccaccg ggatctccgg gtccatgacg
     5521 gtctcgagca ccgcccggat gtcggccgtg gtcacacccg ctccagcgcc agcgcgatcc
     5581 cctgcccgac accgatgcac atggtcgcca gcgccagctt gccgccgttg cccgcaagct
     5641 cctcggccgc ggtcagagcc agccgcgcgc ccgacatccc gagcggatgg ccgagcgcga
     5701 tcgcgccgcc gttggggttc acctgggccg cgtcgtcggg aaggccgagc tggcggagca
     5761 ccgccagcgc ctgggcggcg aacgcctcgt tcagttcgat cacgtccatc tgctcgatct
     5821 tcaggccgag gcgcttgagc agtttctcgg tggcgggcgc cggcccgatg cccatgatcc
     5881 gcggcggcac gccggcgacc gcggcgccga gcacccgggc gcgcggcgtg aggccgttgc
     5941 gccgggccgc ttcctcgctc gcgacgatga tcgccgcggc gccgtcattg actcccgacg
     6001 cattgccggc ggtgacggtg ccgtcggcgc ggacgatcgg cttcaatttg gcgagcgatt
     6061 cgaggctggt ggcgcgcggg tgctcgtcgc gatcgacgac cagcgggtcg cccctgcgct
     6121 gcgggatctc gaccgggacg atctcggcgg cgagccggcc gctggcttga gcggcggcgg
     6181 tgcgctgctg gctgcgcagc gcgaacgcgt cctggtcctc gcgcgtgacc tgaaactcct
     6241 gggcgacgtt ctcggcggtc gaaggcatgg tgtcgtcgcc gtaggagtcg cgcatcttcg
     6301 ggttgacgaa gcgccagccg atggtcgtgt cgtagatctc ggcggtgcgg tcgaacggct
     6361 ggcttgcctt gggcaggacg aacggcgcgc ggctcatgct ttccgagccg ccggcgatga
     6421 tcaggtcgga ttcgccgccg cggatcgcgc gcgcggccat cgccaccgca tcgaggcccg
     6481 agccgcacag gcggttgagg gtcaccccgc ccgagctgtc gggcaagccc gcgagcagcc
     6541 cggccatgcg tgccaggttc cggttgtcct cgccggcctg gttggcgcag ccgaggatga
     6601 cgtcgtcgag ctcgccccag tcgaccccgg ggttgcggcc gatcagcgcg cggatcggga
     6661 ccgcggcgag gtcgtcggcg cgaatgggcg ccagcgcccc gccgtagcgg ccgatcgggg
     6721 tccgcacggc gtcgcagatg aaggcttcgg tcatggccgc gctactccgt cattgcgagc
     6781 gcagcgaagc aatccagctg gattgccgcg tcgccttcgg ctcctcgcaa tgacgacatg
     6841 aaataatcat cccagtcaga ttacgcctcg gcggatctga tcgagctcga tgctctcgaa
     6901 cagcgccttg aaattgcctt cgccaaagcc ctcgttgccc ttgcgctgga tgatctcgaa
     6961 gaagatcggg ccgatcacgt tctgggtgaa gatctgcagc agcagcccct gcccttcggt
     7021 cggggcgccg tccatcaata tgccgcgctt ctggagctcg gggaccgact cgctatggcc
     7081 ctcgatccgg ctgtcgagca gttcgtaata ggtgccgggc gtgtcctgga acggaatgcc
     7141 gcgcgcccgc aaaatgtcga ccgcggcgta gatgtcgtcg gtgccgagcg cgatgtgctg
     7201 gatgccctcg cccttgtact cgcgcaggaa ctcctcgatc tggctcttgt cgtcctggct
     7261 ttcgttcaac gggatgcgga tcttgccgca cggcgaggtc atcgccttgg agaacaggcc
     7321 ggtgaccttc ccctcgatgt cgaagtagcg gatctcccgg aagttgaaga gcttctcgta
     7381 gaagtcggcc cagaccgaca tccggccgcg attcacattg tgggtcagat ggtcgatgta
     7441 ggtgaggcgg gacgcgtggg ccgcctcgcg ctcgcgccag tcggggtgga agtcgaagtc
     7501 gatgtcgtag atcgtgccct tgtcgccgta gcgatcgacg aaatacagcc gcgagccgcc
     7561 gatgccctcg atcgccggga tctcgagctc gcccccggcg acgtcgctct tgacgtcggt
     7621 ggctccgagc cggagcgctc gggcgtgcgc ggccgtcgcg tccttgaccc ggaacgccat
     7681 cgcgcaggcg cacggcccgt ggatcgcggc gaactcctcg gcgaagctgt cgggctcggc
     7741 attgatgatg aagttgcagt cgccctggcg gtggagggtg acgttcttgc gcttgtggcg
     7801 ggcgacctcc gggaagccca tcttggtgaa cagcgagcgc agcagctccg aatccggggc
     7861 ggcatattcg acgaactcga agccgtcggt gcccatcggg ttctccaggc ccagcgggcc
     7921 tttttcggcg acagcggtgg ccattgccaa tcctcctcct tgcgggcgtt tccctgcgct
     7981 cgggcgagcg gctttgcaag attgggccat gggtccatag gaacccgatg gaccgaccgc
     8041 cgcccgatgg ctggaccctc gacgagagcg gcaaggtgct gctgcggacc taccggttcg
     8101 ccgatttcgc cgaggccttc gccttcctca cgcgagtcgc cgagcatgcc gagcgggtca
     8161 accaccaccc ggagttcacg tcggtctgga accgcgtcga tttccggctg acgacgcacg
     8221 attccggcgg gctgacgaac aaggattatg cgctggcgga ggcgatagac gctctggccg
     8281 gccgctgagc gcggcccgat agcgccgtca gcgttgcgac agggtcgccg acagctgggc
     8341 tagcggccgg ccgagcgcct gcccgaccga gttgaagacg gtgaccccga cggccgcccc
     8401 gaacagcgcg acgaccgcga actcgacgct tctcgacagc atccggctgg cgtcgtgccg
     8461 ggcctgggcg cggtgcggcg ctgccctgcg ctcgagcgcc gcctcggccg cgaccgctcc
     8521 cgccagctgg gcgatcgcgg gatcgggcgg gtggatgatc tcttccaccg gcagcgcatc
     8581 ctgggtgcgg atcccgaagc gatggccctc gacccagacg actcgcccga caatcgcata
     8641 ttggccgcgg tggaggtcga gataggtccc ccgggcgggc ggctcggagg tctggacgag
     8701 cagaccgcga gtggagatgt tgaggatgca gacgtcgttc caggtcgcgg cgacccgcat
     8761 ccgcgcgttg agcatgacct tgcggcgcgg ttcgcgagtt ctcaactgca agccatcccg
     8821 tctcgctcgg ccagtcccct gtggcgttgc cgatcgcgtc gcccgctttt cccgaggcaa
     8881 gcacctacag ccgcttaatc atcggcccgt ttcggaggca gtttcacggc ccgcgggagg
     8941 ggcaatggtc ggcaagccgg cgacaaggca gccattgccc gttcatccgg gccgcgccat
     9001 atggggacgg tgatgatccg ctcgtccgcc tcgattgcac gttcgccggc ttcggcccgc
     9061 acgggcgcgc gcggctctta ttatgcctgc gaggccggga ccggctgacg cgcccaagcg
     9121 acagacgaaa ccccagcctc gacccgccaa gcctccgctc ggcgggtttt ttcatgcgcc
     9181 acgaaggaat cccgaccatg cttcacaaag ccatcgccac gacagaccgg ccccgcggct
     9241 ggcgggatta tgtcgtgccg caatgctggg agaggttcac gaacgacgac catgcggtgt
     9301 gggacctgct gttcgcgcgc caggtcgagc tgctcggcag cagggtcgtg gcgccgttcc
     9361 tcgacgggat cggcctgttg cgcctgtccc acccgggagt gcccgacctc gacgagctca
     9421 atgcgatcct cggcccccgc accgggtggc ggacggtggc ggtgaccggg ctggtccccg
     9481 acgacatctt cttcgcgatg ctgagcgagc gggtgttccc ggtcggcaat ttcatccgcc
     9541 ggcgcgaaca gctcgattat ctcgaggagc ccgactgctt ccacgacctg ttcggccata
     9601 tcccgatgct cgcgcaccac gattatgccg aaatgatcga gcatgtcggg cgattgggcg
     9661 gcgcggcgat cgccgccggc cagggcgagc gggtcgcgcg gatctactgg cacagcgtcg
     9721 agttcgggct cgcccgcgaa ggcggcgagc tcaagatcct cggcgccggg ctcgcgtcga
     9781 gcttcggcga ggcccatttc agcctcgaaa gcgagtcggt cgagcggctg cccttctcgg
     9841 tgccgggcgc ggtctgcacc gcctaccgcc acgacgcgtt ccagccgctc tacctggtgt
     9901 ccgaatcgct cgaagcgacg gtcgccgaga tcctcgccac cgacgccgag cggctgctgg
     9961 cgatttgagc ggacgggcgc ggcctgtata gctcggcggc aagcttatcg agggcgcagc
    10021 cgcgcgggga tcgtgacacc ggaccattac gccatcctgg gagtgtcgcc ctcgtcggaa
    10081 gacgtggaga tccggaccgc ttatctcggt ctgatgcggc gctaccaccc ggaccgcaac
    10141 tcctcgcccg aggcggcgga gcgggcgcgg gcgatcacga ccgcttatgc ggtgctcagc
    10201 gattgggacc ggcgcgccga atacgacctc aagcgagccc ggctgcgctc cctggaagcg
    10261 acgatgaacg tcgccccgcg gcggccgccg ccgagcccca gcgcgatgtt cgcggtggcg
    10321 gcggtgtcgc tgctgctgct ggtggcgatc cggccgccgc tgttcgtccg cgagctgccc
    10381 gagcgagcgc cggctgcggc gccggtccac aaggccgccg ccccgaagcc cgatcccgcc
    10441 gcttattgcc ggtcgccggc gacccgcgac cagatccggc gcgaactgtt ccggcgcgcg
    10501 gcccggcttc ggggcaagga ccaggcggcg ttcgaccgga ttgccggcca tgcgctgatc
    10561 cggatcgatt cgcccgagct ccgccatgcg gacgacaagc gcgggaccgt gcgctgcgaa
    10621 gcctgggtcg cgctcgacct gccccccggg gtcgcggtgc tcggcggccg gcggaacctg
    10681 atggccgaca tcggctatac ggtgaagacc gctcgcggcg gccggggctc ggtccgcctg
    10741 tccggcgagg gcctgatcgt gacgccgctg gcgacgctcg cgcaaattgc ccgcccggcg
    10801 gaagagccgc cggaagcgac cgcgatcgaa ccgcggcttg ccgaggcgcg gccgaggctc
    10861 gaccagccgc cgccacccaa accggcgccc gccccgccgc ccgcccggat ggctccgcaa
    10921 cccgcccggg cggcgccgca aatcgccaag gcggcatcgc aaccggcgcg cgccacgcaa
    10981 atcgccgagg cggcggcgca accggcccgg ccgacagcgc aagccgcgac tccccagccc
    11041 aggccgagct tcagctgcca gatcgccagg ggacgcg
//
LOCUS       DASTZO010000040         5288 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_735024,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000040 DASTZO010000000
VERSION     DASTZO010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5288
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735024"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /locus_tag="VFU20_00995"
     CDS             complement(<1..549)
                     /locus_tag="VFU20_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5285069.1"
                     /translation="MSFKPTIIALALASAALGAAPATAQYGAPPPQQQPQIQPPASTA
                     VPGQRQLNISKRAAKAINELQAAVNANDVANIPARLAAAQAVAQSSEEKFYVAQLQLK
                     AATASKNQAAIAAAIEAMLASGGVEQAHVAPLYLELGDIHHKNKQYAQATAAFERVLA
                     LDPRNAQALALLAETRNSQGQPN"
     gene            772..1206
                     /locus_tag="VFU20_01000"
     CDS             772..1206
                     /locus_tag="VFU20_01000"
                     /inference="COORDINATES: protein motif:HMM:NF024600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEU5285070.1"
                     /translation="MRKLALPALLAGAAGLVACAPVAPAGTMAASSVCGTYGYVDVNN
                     DGFISGSEWNTYRTSTYNYWDVNGDGRISRSEFENCWRAGGFYREANYNPDYWTNYWS
                     AFDADGDGYLSADEYWSASTWARIDRNANGRIDSDEWVWWGP"
     gene            1441..4173
                     /gene="gyrA"
                     /locus_tag="VFU20_01005"
     CDS             1441..4173
                     /gene="gyrA"
                     /locus_tag="VFU20_01005"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU5285071.1"
                     /translation="MAPISIVEEMKTSYLDYAMSVIVSRALPDVRDGLKPVHRRILYS
                     AYENGFTYNRGYRKSARIVGDVIGKYHPHGDTAIYDALARMTQSWSMRVPLIDGQGNF
                     GSMDPDPPAAMRYTEARLARVANFLLGDLDKDTVNFQPNYDANETEPQVLPARFPNIL
                     VNGAGGIAVGMATNIPPHNLGEVLAACRAYIDDPEVSSEALMEHVKGPDFPTGAFILG
                     MAGIRSAYTTGRGSILLRSRHKVEEGRGDRVSIVLTEIPYQVGKNGLVEKIAEAAKDK
                     RIEGVSDIRDESNREGVRIVIELKRDATPEVVLNQLWRHTPAQSSFPANMLAIRGGRP
                     ETLTLRDFIESFVKFREEVITRRSKHELARARERAHILLGLVVAVTNLDEVVKLIRGS
                     ASPAQAREKLLEREWPGDQIRPYIALVEAVEPKKAGKSYRLSEAQVKAILELRLHRLT
                     ALGRDEIGTELETLAGSIGELLEILANRARLYEVMRAEFDEVDAEFSTPRLSELAPAA
                     DGIEDEDLIEHEDMVVTVTMAGYIKRTPLAVFREQKRGGKGRSGMATKDEDAVTNLFV
                     TSTHNPVLFFSNLGRVYRMKVWRLPEGGPTAKGRPMVNLLPLAEGEVISTVLPLPEDE
                     AEWGGLHIMFATAHGTVRRNSMDAFTNVPTAGKIAMRFGVSDGEAEGEEAGDPTDRLV
                     GVELLTEEDDVFLATRNGKAIRFMSTDVREFQSRTSTGVRGIRLLGDDQVISMSILRR
                     VGTTQEEREAYLKCPPWREREDVECALPAQRVAELAEKEQFILTVTENGYGKRSSAYE
                     YRRTNRGGQGITNIDTSERNGSVVASFPVKPGEQLMLVTDQAKMIRTTVGDIRIMGRN
                     TQGVTIFRVAENERVVSVAKIEETDDEVEVEASDEVAPDEGAVVGEDLASGSDK"
     gene            4229..4684
                     /locus_tag="VFU20_01010"
     CDS             4229..4684
                     /locus_tag="VFU20_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment family protein"
                     /protein_id="HEU5285072.1"
                     /translation="MALPHQALVLVADGRKLLFLRNHGDQNQIDLRTEAHDFRHDPKD
                     REIKTDAAGSMKQSFGYGRPSMEETDFHQLDKDRWAKDAAEELKRRALRNDFDALAII
                     APPRTLSVLRKELHKEVERRIVQTINKEMTDRPIPDIEELLVGEAAPPA"
     gene            complement(4725..4991)
                     /locus_tag="VFU20_01015"
     CDS             complement(4725..4991)
                     /locus_tag="VFU20_01015"
                     /inference="COORDINATES: protein motif:HMM:NF025392.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEU5285073.1"
                     /translation="MSVFMVERSLGGISMEDLGGAQKAAIDKCEQLSASGTPVRYIRT
                     TFAPEDGRCMCLFEAGSSEDVKRANDEAGLPYDRVVPAMDLTPQ"
     gene            complement(5090..>5288)
                     /locus_tag="VFU20_01020"
     CDS             complement(5090..>5288)
                     /locus_tag="VFU20_01020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEU5285074.1"
                     /translation="HEELRRIYAGQLELPEAIAQGAQMLALAQDRPSALLCFEREPAG
                     CHRSLLLAAVAPSTEVVHLFA"
BASE COUNT          910 a         1805 c         1793 g          780 t
ORIGIN      
        1 attgggctgg ccttgcgaat tgcgggtctc ggcaagcagg gccagcgcct gagcattcct
       61 ggggtcgaga gccagcaccc gctcgaacgc ggccgtcgcc tgcgcatatt gcttgttctt
      121 gtgatggatg tcgccgagct cgaggtacag cggcgcgacg tgggcctgct cgaccccgcc
      181 cgacgccagc atcgcctcga tggccgcggc gatcgccgcc tggttcttcg atgccgtggc
      241 cgccttgagc tgcagctggg cgacgtagaa tttctcctcg ctcgattggg cgacggcctg
      301 ggcggccgcg agcctggccg ggatattggc gacatcgttg gcgttgaccg cggcctgcag
      361 ctcgttgatc gccttggccg cgcgcttcga gatattgagc tggcgctggc cgggcaccgc
      421 ggtcgatgcg ggcggctgga tctgcggctg ctgttgcggc ggcggagcgc cgtactgggc
      481 ggtggcgggc gcggcgccca atgcggccga ggcgagcgcc aaggcgatga tggttggctt
      541 gaaggacatg gaactctccc attcgccggc ggccggcgct ttgcttgacc gttaagggtc
      601 tcgtccggct ccataatccc gctcggggcg acagcaaaga gttagcttgt ccggcgttag
      661 cccgctggcc gctcgttgcg gaacttcaag tccggcctgt gggttttttt cgctaagccc
      721 cggaagagcg ggggaaaaac agcttcgggg tgattcgaaa ggagaatctc gatgcgcaag
      781 cttgctcttc ccgcactgtt ggccggcgcg gccggtctgg tggcctgcgc tccggtcgct
      841 ccggcgggca ccatggccgc cagcagcgtc tgcggcacct acggctatgt cgacgtcaac
      901 aacgacggct tcatcagcgg cagcgaatgg aacacctatc gcaccagcac ctacaattat
      961 tgggacgtga acggcgacgg ccggatcagc cgaagcgagt tcgagaattg ctggcgcgcc
     1021 ggcggcttct accgcgaggc caattacaac ccggattact ggaccaatta ctggagcgcg
     1081 ttcgacgccg acggcgacgg ctatctgagc gccgacgaat attggtcggc gagcacctgg
     1141 gcccggatcg accgcaacgc caacgggcgg atcgactccg acgaatgggt gtggtgggga
     1201 ccgtaaggcc cttgcctcgc cgagcggccg cgctgctcgc gcgggcacgc gcgcgcgggg
     1261 tggcgctcgc ggccctgatc ggctagtccg gcgaaggtgg gggcaggcct tccggccgtt
     1321 cctttcgtgt acccgtaatc tctagctcag gatcgacatc acttggccac cgaaccgccg
     1381 gtcgaggacc gcaacgacag caataatggc ggcgccgggg gctctggcgg cgaccgcgac
     1441 atcgcgccca tctccatcgt cgaggagatg aagaccagct acctcgacta tgcgatgagc
     1501 gtcatcgtca gccgggcgct gcccgacgtc cgcgacggcc tcaagccggt ccaccgccgg
     1561 atcctctact cggcctacga gaacggcttc acctacaacc gcggctaccg caagtcggcg
     1621 cggatcgtcg gcgacgtcat cggcaaatac cacccgcacg gcgacaccgc gatctacgac
     1681 gcgctggcgc ggatgaccca gagctggtcg atgcgggtgc cgctgatcga cggccagggc
     1741 aatttcggct cgatggaccc cgacccgccg gcggcgatgc gctacaccga ggcgcggctc
     1801 gcccgggtcg ccaactttct gctcggcgac ctcgacaagg acaccgtcaa cttccagccc
     1861 aattacgacg ccaacgagac cgagccgcag gtgcttccgg cgcgcttccc caacatcctc
     1921 gtcaacggcg ccggcggtat cgcggtcggg atggcgacca acatcccgcc gcacaatctg
     1981 ggcgaggtgc tggccgcgtg ccgcgcctac atcgacgacc cggaggtcag ctccgaagcg
     2041 ctgatggagc acgtcaaggg ccccgacttc ccgaccggcg ccttcatcct cgggatggcc
     2101 gggatccgca gcgcctacac caccggccgc ggctcgatcc tgctgcgctc gcgccacaag
     2161 gtcgaggaag gccgcggcga tcgggtgtcg atcgtgctga ccgaaatccc ctaccaggtc
     2221 ggcaagaacg ggctggtcga gaagatcgcc gaggccgcca aggacaagcg catcgagggc
     2281 gtttccgaca tccgcgacga gtccaaccgc gagggcgtcc ggatcgtcat cgagctgaag
     2341 cgcgacgcga cccccgaggt cgtcctcaac caattgtggc gccacactcc ggcgcaatcc
     2401 tcgttcccgg ccaacatgct ggcgatccgc ggcgggcgcc cggaaacgct gaccctgcgc
     2461 gacttcatcg agagcttcgt caagttccgc gaggaggtca tcacccggcg ctccaagcac
     2521 gagctggccc gcgcccgcga gcgcgcccac atcctcttgg ggctcgtggt ggccgtcacc
     2581 aacctcgacg aggtggtgaa gctgatccgc gggtcggcga gccccgccca ggcgcgcgag
     2641 aaactgctcg agcgcgagtg gccgggcgac cagatccgcc cgtacatcgc gctggtcgag
     2701 gcggtcgagc cgaagaaggc cggcaagagc taccgattgt ccgaagcgca ggtgaaggcg
     2761 atcctcgagc ttcgcctcca ccggctgacg gcgctcggcc gcgacgagat cggcaccgag
     2821 ctcgagactc tcgccggctc gatcggcgag ctgctcgaga tcctcgccaa ccgcgcccgg
     2881 ctctacgagg tgatgcgcgc cgagttcgac gaggtcgacg ccgaattctc gacgccccgc
     2941 ctcagcgagc tggccccggc cgccgacggg atcgaggacg aggatttgat cgagcacgag
     3001 gacatggtcg tcaccgtgac catggccggc tacatcaagc gcaccccgct ggccgtgttc
     3061 cgcgagcaga agcgcggcgg caagggccgc tcggggatgg cgaccaagga cgaggacgcg
     3121 gtcaccaacc tgttcgtcac ctcgacccac aacccggtgc tgttcttctc caacctcggc
     3181 cgggtctacc ggatgaaggt gtggcgcctg ccggagggcg gcccgaccgc caaggggcgg
     3241 ccgatggtca atctgctgcc gctggccgag ggcgaggtta tctcgaccgt cctgccgctg
     3301 cccgaggacg aggccgagtg gggcgggctg cacatcatgt tcgcgaccgc ccatggaacg
     3361 gtgcggcgca acagcatgga cgcgttcacc aacgtgccca ccgccggcaa gatcgcgatg
     3421 cgcttcgggg tcagcgacgg cgaggccgag ggcgaggagg ccggcgaccc gaccgaccgg
     3481 ctggtcggcg tcgagcttct gaccgaggag gacgacgtct tcctcgccac ccgcaacggc
     3541 aaggcgatcc gcttcatgtc gaccgacgtc cgcgagttcc agtcgcgcac ctcgaccggg
     3601 gtccgcggca tccgcctgct cggcgacgac caggtgattt cgatgtcgat cctgcgccgg
     3661 gtcggcacca cccaggagga gcgcgaggcc tatctcaagt gcccgccgtg gcgcgagcgc
     3721 gaggacgtcg aatgcgcgct tccggcccag cgcgtcgccg aactggccga gaaggagcag
     3781 ttcatcctca ccgtgaccga gaacggctac ggcaagcgat cgtcggccta cgagtaccgg
     3841 cgcaccaacc gtggcggcca gggcatcacc aacatcgaca cgtccgagcg caacggaagc
     3901 gtcgtcgcca gcttcccggt caagccgggc gagcagttga tgctggtcac cgaccaggcc
     3961 aagatgatcc gaacgacggt cggcgacatc cggatcatgg ggcgcaacac ccagggcgtg
     4021 acgatcttcc gggtcgccga gaacgagcgg gtcgtcagcg tcgccaagat cgaggagacc
     4081 gacgacgagg tcgaggtcga agccagcgac gaggtggcgc ccgacgaggg cgcggtcgtc
     4141 ggcgaggatc ttgcgtcggg cagcgacaag taaaggaacg gcggcacggt ccgccgcgtt
     4201 cgctcagcga cacgctgagg agaaagaaat ggcccttccc catcaagcgc tggtgctcgt
     4261 cgccgacggc cgcaagctcc tgttcctgcg caaccacggc gaccagaacc agatcgacct
     4321 gcgcaccgag gcccacgact tccggcacga ccccaaggac cgcgagatca agaccgacgc
     4381 ggcggggagc atgaagcaga gcttcggcta cgggcggccg tcgatggagg agaccgattt
     4441 ccaccagctg gacaaggacc gctgggccaa ggacgcggcc gaggaactga agcgccgcgc
     4501 gctcaggaac gatttcgacg cgctggcgat catcgccccg ccgcggaccc tgagcgtgct
     4561 ccgcaaggag cttcacaagg aggtcgagcg gcggatcgtc cagaccatca acaaggaaat
     4621 gaccgaccgg ccgatccccg acatcgagga gctgctggtc ggcgaagccg ctccgcccgc
     4681 ctaggcggct gccctggggc acccgccttc cgcggcgccc gcggttattg gggcgtcagg
     4741 tccatcgccg gcacgacccg gtcgtaggga agcccggcct cgtcgttggc gcgcttgacg
     4801 tcctcgctcg agccagcctc gaacaggcac atgcagcgcc cgtcctcggg agcgaaggtc
     4861 gtccggatgt agcggaccgg cgtccccgag gcgctcagtt gctcgcactt gtcgatcgcc
     4921 gccttctggg cgccgccaag gtcctccatg ctgatcccgc cgaggctccg ttcgaccatg
     4981 aaaaccgaca tcgatccctc catcgttgcg cgtgaagcga ctaggcgtga tccgcggggg
     5041 gagcaagcaa gcgcggccgg gatcaccgcg cggcatcgct cccgcctcgt caggcgaaca
     5101 ggtgaacgac ctcggtgctc ggcgcgaccg cggccagcag cagcgagcgg tggcagccgg
     5161 cgggctcgcg ctcgaagcac aacagcgcgc tcggcctgtc ttgcgcgagc gcgagcatct
     5221 gcgcgccctg cgcgatcgcc tccggcagct cgagctggcc ggcgtaaatc cgcctgagct
     5281 cctcgtgg
//
LOCUS       DASTZO010000041         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_760562,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000041 DASTZO010000000
VERSION     DASTZO010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760562"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1059
                     /locus_tag="VFU20_01025"
     CDS             <1..1059
                     /locus_tag="VFU20_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5285075.1"
                     /translation="WNLFMPPSHGQPPVDDSFEFDGPGLTNLQYAFCAEEMGRILWAS
                     EAFNCSAPDTGNMEVLHRYGTAEQKQQWLRPLMDGEIRSAFLMTEPDVASSDATNIQC
                     KIAMRGNEYVVSGRKWWSSGAGDPRCRLAIVMGKSDPAADTYRQQSMILVPMDAEGVK
                     VVRHLPVFGYDDAPHGHMEVELDEVRVPASNILLGEGRGFEIAQGRLGPGRIHHCMRM
                     IGVAEEALEAMARRLQSRTAFGKKLAEQSVWEERIADARIDIEMTRLLCLKAADMMDR
                     VGNKAAQGEIAMIKVAAPRTALKVLDDAIQAHGGAGVSEDFGLARAYASVRTLRLADG
                     PDEVHRRAIARIELNKHQ"
     gene            1056..2090
                     /locus_tag="VFU20_01030"
     CDS             1056..2090
                     /locus_tag="VFU20_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEU5285076.1"
                     /translation="MSDPPLDVELDKAALGAWLEANVEGFLAPFELTKFPSGQSNPTY
                     KVAAASGDYVLRRKPFGPLLQSAHAVDREYRLLSALAPLGFPVPRPLALCDDPGVIGA
                     IFYVMELARGRPYANGALPEFDPATRRHMYEELVDTLADLHLIDPQAAELGDFGRPGN
                     YFERQVARWTRQYRDSQTDYLPEMERLIAFLPETLPEQSRTSIVHGDYRIDNVVFDGD
                     GTLTAVLDWELATLGDPVADFSYLAMQWVMPADGGAALSGLDLKALGIPSLEEMVERY
                     SQRSGVPVAASLNWYFAYNLFRLAGIVQGIKKRLIDGTASHSNAAEIAAKVPSLARAA
                     WGFALSTQGK"
     gene            2281..2868
                     /locus_tag="VFU20_01035"
     CDS             2281..2868
                     /locus_tag="VFU20_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285077.1"
                     /translation="MKAVSKILAGGIGLVALAGAAPAAAQYYPGYGYGGGNVLGQVLN
                     QVLGGSQYGYGIHPQAAVQRCAAAVEQRINRDYRYNSAYGGYGGYGGYGGYGYNSYAG
                     GTARVLGVTSVERRSNNGLRVRGVATANAYASGYSPYGGYGGYGGYGGYGGGYGGYAP
                     QQSAELTFKCNVDYRGYITDIDVDRNTYAYGYRRY"
     gene            3052..3675
                     /locus_tag="VFU20_01040"
     CDS             3052..3675
                     /locus_tag="VFU20_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285078.1"
                     /translation="MEDKAVQAGAAETRAAARTNLFLAASLLSAGVAHPVRIRDLSAW
                     GARVETAVVPEVGTEVRLVRGRLSIACLVTWSAEHFFGLRFASPVSVLEWMTHRVVQE
                     RRRIDDLAVANRDTAAEVAAARLETEKAARAARDLSRVSRLLESLGEALAGDPQVVAR
                     HGVKLHTLGLAMQTLAALAETIQASARDGAAAAGAAAGGSARAKRRA"
BASE COUNT          623 a         1227 c         1313 g          583 t
ORIGIN      
        1 tggaacctgt tcatgccgcc gagccacggc cagcctccgg tcgacgacag cttcgagttc
       61 gacgggcccg gcctgaccaa cctccaatac gcgttttgcg ccgaggagat ggggcgaatc
      121 ctgtgggcga gcgaggcgtt caactgctcc gctcccgaca ccggcaacat ggaggtgctg
      181 caccgctacg ggacggctga gcagaagcag caatggctca ggccgctgat ggacggggaa
      241 atccgctcgg cgttcctgat gaccgagccc gacgtcgctt cgtccgacgc gaccaacatc
      301 cagtgcaaga tcgcgatgcg cggcaacgaa tatgtggtca gcggccgcaa atggtggtcg
      361 agcggcgccg gcgacccgcg ctgcaggctg gcgatcgtga tgggcaagag cgacccggcc
      421 gccgacacct accggcagca gtcgatgatc ctggtgccga tggacgccga gggggtgaag
      481 gtcgtccgcc atttgccggt gttcggctac gacgacgctc cgcacggcca tatggaggtc
      541 gagctcgacg aggtgcgcgt gccggcgtcg aacatattgc ttggcgaagg gcgcgggttc
      601 gagatcgcgc agggccggct gggccccggg cggatccacc actgcatgcg gatgatcggg
      661 gtcgcggagg aggcattgga ggcgatggcc cggcggctcc agtcgcggac cgcgttcggc
      721 aagaaactgg ccgagcagtc ggtgtgggaa gaacggatcg ccgacgcgcg gatcgacatc
      781 gagatgaccc ggctgctgtg cctgaaggcc gcggatatga tggaccgggt cggcaacaag
      841 gcggcgcagg gcgagatcgc gatgatcaag gtcgccgccc cgcgaaccgc cctgaaggtg
      901 ctcgacgacg cgatccaggc gcacggcggc gccggcgtgt ccgaggattt cgggctggct
      961 cgggcctacg cgtcggtgcg caccctgcgg ctcgccgacg ggcccgacga ggtccaccgg
     1021 cgggcgatcg cgcggatcga gctcaacaag caccaatgag cgatccgcct ctcgacgtag
     1081 agctcgacaa ggcggcgctg ggagcgtggc tcgaggcgaa tgtcgagggt ttcctggccc
     1141 cgttcgagct gaccaaattc ccgtcgggcc agtccaaccc gacctacaag gtcgccgccg
     1201 cgtcgggcga ctatgtcctt cgccgcaagc cgttcgggcc gcttctgcag agcgcccacg
     1261 cggtcgaccg cgagtatcgc ttgctgtcgg cgctggcgcc gctcggcttc ccggtgccgc
     1321 ggcccctcgc tttgtgcgac gacccggggg tgatcggcgc gatcttctac gtgatggagc
     1381 tggccagggg ccggccctac gcgaacggcg ccctgcccga gttcgacccg gcgacccggc
     1441 ggcacatgta cgaggagctg gtcgacacgc tcgccgacct gcacctgatc gacccgcagg
     1501 ccgcggagct cggcgatttc ggcaggccgg gcaattattt cgagcgccag gtggcgcgct
     1561 ggacccgcca gtaccgcgat tcgcagaccg attacctgcc cgagatggag cggctgatcg
     1621 ccttcctgcc cgaaactctc cccgagcagt cgcgcacgtc gatcgtccat ggcgactatc
     1681 gaatcgacaa tgtcgtgttc gacggcgacg gcacgctgac cgcggtgctc gactgggagc
     1741 tggcgacgct cggcgacccg gtcgcggact tctcctattt ggcgatgcag tgggtgatgc
     1801 cggccgacgg cggcgcggcc ttgagcgggc tcgacctgaa ggcgctcggg atcccgtcgc
     1861 tcgaggaaat ggtggagcgc tactcgcagc gttcgggagt gccggtcgcg gccagcctca
     1921 actggtattt cgcctataat ctgtttcgcc tcgcggggat cgtccagggg atcaagaaaa
     1981 ggctgatcga cggcaccgcg agccattcca acgcggccga gatcgcggcc aaggtgccaa
     2041 gcctggcccg cgccgcgtgg ggcttcgcgc tgtccaccca gggcaaatga agcaccgccg
     2101 gctcgggact tgcccataag ttccaggatt cggcgtttga aagccgtggc ttggcgcgat
     2161 ttcctgaacg ccagaaaacg gtgcattcag ccgccgtgca acgctgttcg tccatgaacc
     2221 ccgacaggac cgccgtgcgt ttgttcggcg agccatttgg taggatcaag gagagtggcc
     2281 atgaaggctg tttcgaaaat tctggcaggc ggcatcggac ttgtcgccct ggccggtgcg
     2341 gcacctgcgg ccgctcaata ttatccgggc tacggctacg gcggaggcaa tgtcctcggc
     2401 caggtgctca accaggtgct tggcggaagc cagtacggct acgggatcca tccccaggcg
     2461 gccgtccagc gttgcgcggc cgcggtcgag cagcggatca accgcgacta tcgctacaac
     2521 agcgcgtacg gcggttacgg cggttacggc ggttacggcg gctatggcta taacagctat
     2581 gccggcggca ccgcccgggt gctcggagtg accagcgtcg agcggcgctc gaacaacggc
     2641 cttcgggtcc gcggagtcgc caccgccaac gcctatgcga gcggctacag cccgtacggt
     2701 ggctatggcg gttacggcgg ctatggtggc tacggcggcg gttacggcgg ctatgccccg
     2761 cagcagtcgg ccgagctgac cttcaagtgc aacgtcgact atcgcggcta catcaccgac
     2821 atcgatgtcg accgcaacac ctacgcctac ggctatcgcc gctactaagg cgatcgcccg
     2881 tcttgggtga aacgaggatg cgccggagtc ccgcgactcc ggcgcatttt tcgtcggttg
     2941 cgcgcgccgg ctgcgatgca tccgaaacgg cccgaaccgc agcaactgct taagcgcccg
     3001 gcgggcaaag ctcgaagccg gcgacatggg ccggcggcat ggagcgcgaa agtggaagac
     3061 aaggcggttc aagccggtgc ggccgagacg cgagcggcgg cgcggaccaa cctgttcctg
     3121 gccgcgagcc tgctttcggc cggggtcgct catccggtgc ggatccggga cctgtcggcg
     3181 tggggcgcgc gagtcgagac cgccgtcgtt cccgaagtcg gcaccgaggt gcgcctcgtc
     3241 cgcgggcggc tcagcatcgc ttgcctcgta acctggtcgg cggagcactt cttcggactg
     3301 cgctttgcct cgccggtctc ggtgctcgaa tggatgaccc atcgagtggt gcaggaaagg
     3361 cggcgcatcg atgacctcgc cgtggccaac cgggacaccg ccgccgaggt cgcggccgcc
     3421 cgtctcgaaa ccgagaaagc cgcccgcgcc gcacgcgacc ttagccgggt gtcgcgcctg
     3481 ctcgaatcgc tcggcgaagc cctggcgggc gatccgcagg tcgtcgcccg gcacggcgtc
     3541 aagctgcata ccctcgggct cgcgatgcag acgctggccg cgctggccga aacgatacag
     3601 gcaagcgccc gcgacggcgc ggcagccgcg ggagccgcgg cgggcgggtc ggcgcgcgcc
     3661 aagcgccgcg cctgaaatta ttaggcgagc atcgcgagca gcgcgaaggt cgccagccag
     3721 tgctcgccca tatagtcgcc ggcgac
//
LOCUS       DASTZO010000042         7833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1282438,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000042 DASTZO010000000
VERSION     DASTZO010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7833
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282438"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..438
                     /locus_tag="VFU20_01045"
     CDS             <1..438
                     /locus_tag="VFU20_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEU5285079.1"
                     /translation="ERAVAMLVGIEKSVVEGAGAAGLAAMLADPGRFAGRKVGTVLAG
                     GNIDTHLLANVLVRDLVRSGRIARLRIAAQDRPGALAAITAKFEAAGVNIIEINHSRI
                     FTRLPAKDTVIEVECEARDPQAIDEVVERLESAGFHVERASLD"
     gene            573..1385
                     /locus_tag="VFU20_01050"
     CDS             573..1385
                     /locus_tag="VFU20_01050"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HEU5285080.1"
                     /translation="MSAPFRFPKFFVTNPSPCPYLPGKIERKVFTELSGRQASELNEA
                     LGRIGFRRSQSVAYRPSCIDCSACVSVRVLAAEFEPSATQRKLIRRHSDLEVTACKPW
                     TTEEQYELLRRYLADRHPGGGMAEMDESDFADMVEQTPVRTFVIEYREPSKDGRPGKL
                     VGACLSDQQGDGLSMIYSFFDVGPAARKGLGTFIILDHIVRAARAGLPYVYLGYWVDG
                     SPRMAYKTGFRPLERLGRDGWRRMDPHAETKIEPAPVELPVRRESKRLLIDA"
     gene            1405..1602
                     /locus_tag="VFU20_01055"
     CDS             1405..1602
                     /locus_tag="VFU20_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285081.1"
                     /translation="MNSVKPTDEQIDYAVSAWHDGGGFGMELYEYLGWSRDEYARWIE
                     NYEVPARPLALGAARNTGDRS"
     gene            complement(1709..2431)
                     /locus_tag="VFU20_01060"
     CDS             complement(1709..2431)
                     /locus_tag="VFU20_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5285082.1"
                     /translation="MRVLLIEDEPTTAKSIELMLGTEGFNVYTTDLGEEGLDLGKLYD
                     YDIILLDLNLPDMHGYDVLKKLRTAKVGTPVLILSGIGEMDSKVRALGFGADDYVTKP
                     FHRDELVARIHAIVRRSKGHSQSVIRTGKIAVNLDAKTVEVDGSRVHLTGKEYAMLEL
                     LSLRKGTTLTKEMFLNHLYGGMDEPELKIIDVFICKLRKKLSLACGGANYIETVWGRG
                     YVLRDSESDEVPEGTEVQAAVA"
     gene            2672..3319
                     /locus_tag="VFU20_01065"
     CDS             2672..3319
                     /locus_tag="VFU20_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HEU5285083.1"
                     /translation="MLEDRILLIDGEAIVIDKPAGLPVDPPRRGGDSVVGRIDELKCG
                     FRRPPVVMHRLDQDTSGCLLLARNPRARAAFQKAFEDRGVEKYYLAVVGSENAEEEGV
                     IDVPVGKRSSAEAGWRMVADPAGQRAVTRWRKLAARGGRTLVEFQPLTGRTHQIRVHA
                     REAFGSGIAGDRVYGIPGGAMLLHASRLVVPRGAKPAIDVTAPLPEHFGEWRDAR"
     gene            3309..3719
                     /gene="arfB"
                     /locus_tag="VFU20_01070"
     CDS             3309..3719
                     /gene="arfB"
                     /locus_tag="VFU20_01070"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HEU5285084.1"
                     /translation="MRVEDVVIPEDALSESFLAATGPGGQNVNKVATAVQLRLDVGKL
                     GLTPGVYERLKRIAGSKLTAAGELLVTARRYRTQDANRTDARARLARMIADAHIVQAK
                     RRPTRPSKAARAKRVEKKVQRGAVKRARGRVELD"
     gene            3719..4210
                     /locus_tag="VFU20_01075"
     CDS             3719..4210
                     /locus_tag="VFU20_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0033745 - L-methionine-(R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5285085.1"
                     /translation="MHDFRIDAGDKAAMYRDLETALAGLVDGEPDPVANMANAAALIW
                     ETLPDLNWAGFYRNVAGENGPELVLGPFQGRPACIRIAFGEGVCGAAAASLDVQRVDD
                     VHAFPGHIACDSASASELVVPIVKDGELVAVLDLDSPTRARFDAVDEAGCVKLAEVLA
                     KAL"
     gene            complement(4289..4744)
                     /locus_tag="VFU20_01080"
     CDS             complement(4289..4744)
                     /locus_tag="VFU20_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5285086.1"
                     /translation="MRTYLVVIDDSPEARVALRFAARRAAKTEGRVEVLAVVEPQDFL
                     QWGGVEKAFEEEQRLRIEGVVSASVGEILDESGVEASVVVRQGEPVKAVREHIGTRAD
                     VAALVLGAAPSGDPGPLVGHFTGNDAGKLPCPVMLIPGGLSDERLEQLS"
     gene            4889..6223
                     /locus_tag="VFU20_01085"
     CDS             4889..6223
                     /locus_tag="VFU20_01085"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex dihydrolipoamide
                     acetyltransferase"
                     /protein_id="HEU5285087.1"
                     /translation="MPTELKMPALSPTMEEGTLAKWLVKEGDAVSSGDILAEIETDKA
                     TMEFEAVDEGTIAKILVPEGSDGVKVGTPIALLAAEGEDTSGAAAGGGSQSEAGAAAK
                     APAPKPDATPAAASKAEEPAKPAPAPPPQAPVETPAAPAAPAVAPGRAEGERIKASPL
                     ARRLAEAQGIDLATLKGSGPGGRIVRADLGAAAGGAMAAPGAAAAPRAAPVEPGEIPH
                     EVVKLSNMRRTIARRLTESKQQVPHIYLTVDVQLDALLKLRSELNAGLEARNVKLSVN
                     DLLIKALAHALIEVPECNVSFAGDNLIKYSRADISVAVSIPGGLITPIVANADGKSVS
                     AISSEMKDLAARAKDGKLQPHEYQGGTASLSNMGMYGIKQFEAVINPPQGMIMAIGAG
                     EKRPYVINDSLQIATVMSATGSFDHRAIDGADGARLMQAFKRLVEAPLGMLA"
     gene            6295..6639
                     /locus_tag="VFU20_01090"
     CDS             6295..6639
                     /locus_tag="VFU20_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285088.1"
                     /translation="MGYVENLKGDGSLLGILDATGGWLLGTLAPAWFFLTFFFLPVVT
                     LLQWKDVRSPLLYILGGGLGGASAMVVFVWPAAYWAWLWLLVVPGCLAGITWWLLVVR
                     RDPNVALQPIHD"
     gene            6672..6743
                     /locus_tag="VFU20_01095"
                     /pseudo
     CDS             6672..6743
                     /locus_tag="VFU20_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663897.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
     gene            6809..7198
                     /locus_tag="VFU20_01100"
     CDS             6809..7198
                     /locus_tag="VFU20_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285089.1"
                     /translation="MLGFLILVVTGPMEFAGMRDPVMELAVVAAVVLFWAPAFAFIPA
                     AILGWFVEQPKARRMIARRSGGLAPHLAISIVAALLLWLLFRTGLFITASTPQLMDGP
                     SLALFALVGLCSGVSWWFMVVEPGRRK"
     gene            7195..7578
                     /locus_tag="VFU20_01105"
     CDS             7195..7578
                     /locus_tag="VFU20_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HEU5285090.1"
                     /translation="MTREPAIRVTAMPADTNPYGGVFGGWLMGQMGLACGSFASRHTR
                     GKAILVAADAVKFPGAMAVGDELSVYVELMSQGRTSLKLKAEAVARERDGEGEKPVAE
                     GEFTFVALDSNDKPRAIAGQEQGNG"
     gene            7571..>7833
                     /locus_tag="VFU20_01110"
     CDS             7571..>7833
                     /locus_tag="VFU20_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5285091.1"
                     /translation="MAESYDLIVLGSGPGGYVAAIRAAQLGLKTAIVEREVLGGICLN
                     WGCIPTKALLRSAEIFHHSKHAERFGLSAGKPGFDLAKMVSRS"
BASE COUNT         1265 a         2595 c         2689 g         1284 t
ORIGIN      
        1 gagcgggcgg tggcgatgct ggtcgggatc gagaaaagcg tcgtcgaggg ggccggcgcg
       61 gccgggctcg cggcgatgct cgccgatccg gggcgcttcg ccgggcgcaa ggtcggcacc
      121 gtgctcgccg ggggcaacat cgacacccat ttgctcgcca acgtcctggt ccgcgacctc
      181 gtccgaagcg ggcggatcgc caggctccgg atcgccgccc aggaccgccc gggcgcgctg
      241 gccgcgatca ccgccaagtt cgaggcggcg ggagtcaaca tcatcgagat caaccacagc
      301 cgcattttca cgcggcttcc cgccaaggac acggtaatcg aggtcgagtg cgaggcgcgc
      361 gacccgcagg cgatcgacga ggtcgtcgag cgactcgagt cggcgggttt ccacgtcgag
      421 cgcgcgtcgc tcgactgagc tgcgcgcaag attcgaccat cccactccgg gacagtcgcc
      481 tccttaactc tctttgcgca atgcaagggt ctttccaacc gcgtttacca gcagcataaa
      541 ggagtccgta accaagccag agagggggtg cagtgagcgc accattccgc tttccgaagt
      601 tcttcgtgac caacccgtcg ccgtgcccct atctgccggg caagatcgag cgaaaggtgt
      661 tcaccgaact gagcggacgc caggcgagcg agctcaacga ggcgctcggc cggatcggat
      721 tccggcgcag ccagtcggtc gcctaccggc cgagctgcat cgattgctcg gcgtgcgtgt
      781 cggtgcgggt gctggccgcc gagttcgagc cgagcgcgac ccagcgcaag ctgatccgcc
      841 gccattccga tcttgaggtc accgcctgca agccgtggac caccgaagag cagtacgagc
      901 tgctgcgccg ctacctcgcc gaccgccacc cgggcggcgg catggcggag atggacgaga
      961 gcgacttcgc cgacatggtc gagcagacgc ccgtgcggac cttcgtcatc gaatatcgcg
     1021 agccctcgaa ggacggccgc cccggcaagc tggtcggcgc ctgcctttcc gaccagcagg
     1081 gcgacggcct gtcgatgatc tacagcttct tcgatgtcgg cccggccgcc cgcaagggcc
     1141 tcggcacctt catcatcctc gaccatatcg ttcgcgcggc gcgcgccggg ctgccttacg
     1201 tctacctcgg ctattgggtc gatggctccc cgcgcatggc ctacaagacc ggcttccggc
     1261 cactggagcg gctcggccgc gacggctgga ggcggatgga cccgcacgcc gagaccaaga
     1321 tcgagccggc gccggtcgag ctgccggtcc gccgcgaaag caagcgcctg ctgatcgacg
     1381 cctgaaagag aacgcccgac ggcagtgaac tcggtgaagc ctacggacga gcagatcgac
     1441 tatgccgtga gtgcgtggca cgacggcgga gggtttggca tggaactgta cgaatatctt
     1501 ggctggagcc gggatgaata tgcccgctgg atcgaaaatt atgaggtgcc ggctaggcca
     1561 ttggctttag gagccgcgcg aaacacgggc gatcgatcct gatccgactg acttctctca
     1621 gtcgtgtttg caaacaacat gatcaccaaa gaaaaaggcg gagcgcccga aagcgccccg
     1681 cctcttcgtt gcccccaacg aaacctgatc aggcgaccgc ggcctggacc tccgtgcctt
     1741 ccggaacctc gtccgactcg ctgtcgcgca gcacgtagcc gcgtccccac accgtctcga
     1801 tgtagttggc gccgccgcag gcgaggctca atttcttgcg cagcttgcag atgaacacgt
     1861 cgatgatctt gagttcgggc tcgtccatgc cgccgtagag atgattgagg aacatctctt
     1921 tggtgagggt cgtgcccttg cggagcgaca acagctccag catcgcatat tccttgccgg
     1981 tcagatgcac ccggctgccg tcgacctcga ccgtcttggc gtcgaggttg accgcgatct
     2041 tgccggtgcg gatgaccgat tgcgaatggc ccttggaacg gcgcacgatc gcgtggatcc
     2101 gggcgaccag ctcgtcgcgg tggaacggct tggtgacgta atcgtcggcg ccgaagccca
     2161 gcgcccggac cttgctgtcc atctcgccga tgcccgacag gatcaggacc ggagtgccga
     2221 ccttggcggt ccgcagcttc ttgagcacgt cgtagccgtg catgtccggc aggttcaggt
     2281 cgagcaggat gatgtcgtag tcgtacagct tgcccagatc gaggccttcc tcgcccagat
     2341 cggtcgtgta gacgttgaag ccctcggtgc cgagcatcag ctcgatgctc ttggcggtcg
     2401 tcggctcgtc ttcgatgagc agcacgcgca ttcgctgatt ccccccgatc gcggcccggc
     2461 tcacgctctc gcgtaatacc gtcttaatgg ccttgaagcg tccattaacc attcgaggga
     2521 tgaagggtaa aggttaatat tgtgtaacag cttggaatcc ttgggctcgt tgcgcgcgag
     2581 tcacaaaggc cccgagaccg cgatcggagc caggcattcc agccatgcaa gtggattgct
     2641 ttggcccttc gctccccgca atgacgaacc aatgctcgag gaccgcatcc tgttgatcga
     2701 cggcgaggcg atcgtgattg acaagccggc agggctgccg gtcgacccgc cgaggcgcgg
     2761 cggcgacagc gtcgtgggcc ggatcgacga gctcaaatgc ggtttccggc ggccgccggt
     2821 ggtgatgcac cggctcgacc aggatacctc gggatgcctt ctgctggccc gcaatcccag
     2881 ggcgcgcgcg gcgttccaga aagcgttcga ggatcgcggg gtcgagaaat attatctggc
     2941 cgtggtcggc agcgagaatg cggaggagga gggcgtgatc gatgtgccgg tgggcaaaag
     3001 atcgagcgcc gaggccgggt ggcggatggt cgccgacccg gccgggcagc gggcggtgac
     3061 ccgctggcgc aagcttgccg cgcgcggcgg ccggacgctg gtcgagttcc agcccctgac
     3121 cggccgcacc caccagatcc gcgtgcatgc gcgcgaggcg ttcgggagcg gcatcgccgg
     3181 cgaccgcgtg tacggcatcc cgggcggagc gatgctgctg cacgcctcgc ggctggtcgt
     3241 gccgcgcggc gccaagccgg cgatcgacgt caccgcgccg ctgcccgagc atttcgggga
     3301 atggcgcgat gcgcgttgag gacgtggtca tccccgagga cgcgctcagc gaaagcttcc
     3361 tcgccgcgac cgggccgggc ggccagaacg tcaacaaggt cgcgaccgcg gtccagctgc
     3421 ggctcgacgt cggcaagctc ggcctcaccc ccggcgtcta cgagcggctg aagcggatcg
     3481 ccggcagcaa gttgaccgcg gccggcgagc tgctggtcac cgcccggcgc taccggaccc
     3541 aggacgccaa ccgcaccgac gcgcgggcgc ggctggcgcg gatgatcgcc gatgcccata
     3601 tcgtccaggc caagcggcgg ccgacccggc cgagcaaggc ggcccgcgcc aagcgggtcg
     3661 agaagaaggt gcagcgcggc gccgtcaagc gcgcgcgcgg gcgggtggag ctggactgat
     3721 gcacgacttc cggatcgatg cgggcgacaa agcggcgatg taccgcgacc ttgaaaccgc
     3781 gctggccggg ctggtcgacg gcgagccgga cccggtcgcc aacatggcca acgccgcggc
     3841 cctgatctgg gagaccctgc ccgacctcaa ctgggcgggc ttttaccgca acgtcgccgg
     3901 agaaaatggg cccgagctgg tgctcggccc gttccagggc cggccggcgt gcatccgcat
     3961 cgcgtttggg gagggcgtgt gcggagcggc ggccgcgagc ctcgacgtgc agcgagtcga
     4021 cgacgtccac gccttccccg gccacatcgc ctgcgactcc gcgtcggcga gcgagctggt
     4081 ggtgccgatc gtcaaggacg gcgagctggt cgcggtgctc gacctcgaca gcccgacgcg
     4141 ggctcggttc gatgccgtgg acgaagccgg ctgcgtgaag ctggcggaag tgctggcgaa
     4201 ggcgctttag ttccccggcg caggccgggg tccagggcgg tgcgaagtag cgctatcgcg
     4261 ctcctgggcc ccggctttcg ccgggggact aggataattg ctccaaccgc tcgtcgctga
     4321 ggccgccggg gatcagcatc accgggcacg gcagcttgcc ggcgtcgttg ccggtgaaat
     4381 ggccgaccag cggcccggga tcgccgctcg gagcggctcc gagcaccagc gcggcgacgt
     4441 cggcgcgcgt gccgatatgc tcgcgcaccg ctttcaccgg ctccccctgg cggacgacga
     4501 ccgaggcctc gactcccgac tcgtcgagga tctcgccgac cgaagcgctg accacgcctt
     4561 cgatgcgcag ccgctgctcc tcctcgaacg ccttctcgac tccgccccac tggaggaagt
     4621 cctgcggctc gaccaccgcg agcacctcga cccggccctc ggtcttggcc gcgcgccggg
     4681 cggcgaagcg cagcgccacg cgcgcctcgg ggctgtcgtc gatgacgacc agatatgtgc
     4741 gcagcttgtc cttcgccccc atgagcctgc actgctgcgt caaaggccgg ccgagcgcaa
     4801 gccggtcaag cttatttggc cgcccacttg accccaaccg ccgcgttcgc tttgtgccac
     4861 tggcctcccg ccacgcggaa agacgccgat gccgaccgaa ctgaagatgc ccgccttgtc
     4921 cccgaccatg gaggagggga cgctcgccaa atggctggtc aaggaaggcg acgcggtcag
     4981 ctcgggcgac atcctcgccg agatcgagac cgacaaggcg acgatggagt tcgaggcggt
     5041 cgacgagggc acgatcgcga aaatcctggt ccccgagggc agcgacgggg tgaaggtcgg
     5101 cactccgatc gctctgcttg cagccgaagg cgaggatacg agcggcgctg ctgccggggg
     5161 cggcagccaa agcgaggctg gcgcggcggc caaggctccc gctcccaagc cggacgcgac
     5221 gccggccgcg gcgtccaagg ccgaggagcc tgccaagccg gctcccgcgc cgccgccgca
     5281 ggctccggtc gagactccgg ccgcgcccgc cgcaccggcg gtcgctcccg gtcgagcaga
     5341 gggcgaacgc atcaaggcga gcccgctcgc gcggcggctg gctgaggcgc agggcatcga
     5401 cctcgcgact ctcaagggct cggggcccgg cgggcgaatc gtgcgcgccg acctcggtgc
     5461 cgccgccggc ggggcgatgg cggcgcctgg ggccgctgcg gctccgcgcg ccgcgccggt
     5521 cgagccgggc gagatcccgc acgaggtcgt caagctgtcg aacatgaggc ggacgatcgc
     5581 ccgccggctg accgagtcga agcagcaggt cccgcacatc tatctgaccg tcgacgtcca
     5641 gctggacgcg ctcttgaagc tgcggtccga gctcaacgcc gggctcgagg cgcgcaatgt
     5701 caagctgtcg gtcaacgatt tgctgatcaa ggcgctcgcc cacgccttga tcgaggtgcc
     5761 cgagtgcaat gtcagcttcg ccggcgacaa tttgatcaag tacagccgcg ccgacatttc
     5821 ggtcgcggtg tcgatcccgg gcgggctgat caccccgatc gtcgccaacg ccgacggcaa
     5881 gagcgtgtcg gcgatctcca gcgagatgaa ggacctcgcc gcccgcgcca aggacggcaa
     5941 gctgcagccg cacgaatatc agggcggcac cgcgtccctg agcaacatgg ggatgtacgg
     6001 gatcaagcag ttcgaggcgg tgatcaaccc gccccaaggc atgatcatgg cgatcggcgc
     6061 cggcgagaag cgcccctatg taatcaacga ttccctgcag atcgcgacgg tcatgtcggc
     6121 gaccggaagc ttcgaccacc gcgccatcga cggcgccgat ggggcgcggc tgatgcaggc
     6181 gttcaagcgc ctggtcgaag cgccgctggg gatgctggct tgatgctcag gtttggagca
     6241 gtggttgtaa cttggctcgt tgcgagtgtc gccgccggaa tcgcgtctac tctgatgggt
     6301 tacgtcgaaa atctgaaggg tgacggaagc cttctgggca ttcttgatgc gacgggaggc
     6361 tggctactcg ggactttggc tccggcctgg ttctttctga cgttcttctt tctacctgtg
     6421 gtcaccctgc tgcagtggaa ggatgttcgt tcgccactcc tctatatcct cggtggaggt
     6481 ctcggcggag cctcggcaat ggtggttttc gtttggccgg ctgcgtattg ggcttggctg
     6541 tggctgctcg ttgttcccgg ctgcttggca ggcatcactt ggtggctttt ggttgtccga
     6601 cgagacccga acgtcgcttt acagccaatc cacgattgag atggagtctc cccctcatcc
     6661 gcgtcactgc catgcctgct gacctcaacc cgtacggcgc cgtgttcggg gtgtggctga
     6721 tgggacagat ggagatggcg gcttgacgcc tgcccaattg cgctcgcgcg ggtttgcggc
     6781 cgttgtggcc gcctcggtcg cgtcctccgt gctgggtttc ctgatcctgg tggtcaccgg
     6841 cccgatggaa ttcgccggaa tgcgcgaccc ggtcatggag ctcgcggtgg tcgccgccgt
     6901 cgtcctgttc tgggcccccg ccttcgcgtt cataccggcc gcgatcctcg gctggttcgt
     6961 cgagcagcca aaagcccggc ggatgatcgc ccgccgctcg ggcggtctcg cgccgcacct
     7021 ggcgatcagc atcgttgcgg cgctgctgct ctggctgttg ttccgcaccg ggctgttcat
     7081 caccgcctcc acgccgcagc tcatggacgg accgagcctg gcattgttcg cgctggtcgg
     7141 cttgtgctcg ggcgtgtcgt ggtggttcat ggtcgtcgag ccggggcggc gcaaatgacc
     7201 cgcgagcccg caatccgggt gaccgccatg ccggccgaca ccaaccccta cggcggcgtg
     7261 ttcggcggct ggctgatggg gcagatgggg ctggcctgcg gctcgttcgc gtcgcgccac
     7321 acgcgcggca aggcgatcct ggttgccgcg gacgcggtca aattccccgg cgcgatggcg
     7381 gtcggcgacg agctcagcgt ctatgtcgag ctgatgagcc agggccgcac gtcgttgaaa
     7441 ctgaaggccg aagccgtcgc ccgcgagcgc gacggcgagg gcgaaaagcc ggtcgcggaa
     7501 ggcgagttca ccttcgtcgc gctcgactcg aacgacaagc cgcgggcgat cgcgggacag
     7561 gaacagggca atggctgaaa gttacgacct tatcgtgctc ggctcggggc cgggcggcta
     7621 tgtcgcggcg atccgggcgg cgcagctggg cctcaagacc gcgatcgtcg agcgcgaagt
     7681 gctcggcggg atctgcctca actggggctg catcccgacc aaggcgctgc tgcgctcggc
     7741 cgagatcttc caccacagca agcatgccga acgcttcggc ctgtcggccg gtaagccggg
     7801 cttcgacctc gccaaaatgg tctcgcgcag cca
//
LOCUS       DASTZO010000043         3212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_473964,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000043 DASTZO010000000
VERSION     DASTZO010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3212
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_473964"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VFU20_01115"
     CDS             complement(<1..191)
                     /locus_tag="VFU20_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU5285092.1"
                     /translation="MAGGQQIIDVRLGPDHAGWRLDRALAAAVPTLSRERLKALIRSG
                     ALTPPVRDPATRVKGCEAFR"
     gene            279..920
                     /locus_tag="VFU20_01120"
     CDS             279..920
                     /locus_tag="VFU20_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HEU5285093.1"
                     /translation="MNAVDLASLLCSRLCHDLMSPVGALNNGIELMADETDPEMLDKC
                     LELLGESARASANKLKFFRLAFGAAGGFGDSIDTHEAQAALEGVFGPERRIELGWMVP
                     GDHLPKGAVKLLLNLALLAGDALVRGGRLDVGAESTDELLELVIRAEGPRILLDPTLR
                     ATLLNGEANGTVEPRAAGAWLAHSLAAEAGGSIRLSDPADAVLMMGATLPRQR"
     gene            1025..>3212
                     /locus_tag="VFU20_01125"
     CDS             1025..>3212
                     /locus_tag="VFU20_01125"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEU5285094.1"
                     /translation="MDDLIADFVAECREMLESLGGEIVAWEAAPDDRARLDSIFRFVH
                     TVKGNCGFFEFPRLEALSHAAEDALADVRAGRRQADGALVSAVLAIIDRIGEMVAAID
                     SGSDMPAGDDSALIAALEPGTDGAVAPVAATVAEAQGKIAAPRTIRLSVELLDRVMSG
                     VSDMVLARNELARRLRESPTDVPVDGAFERLSSIIAEMRDAITRTRMQRIENLFVGLP
                     RMVRDLSAELGKQVLVDIEGGDVELDREMIEMIRDPLTHIIRNAVDHGIETPAERLKA
                     GKREIGILCVSARQSGNQILIDIQDDGRGIDGKKLVEKAIAAGTLTKAAAAEMSPREQ
                     LALIFEAGLSTASSVTAISGRGVGMDVVRSNVERIGGLVEVDSRPGVGTRMTLRVPLT
                     LTIIPALTVSIGSQHFAIPRSAIDEIVRANGESVKLERVGGSGIATIRGRRVPQIVLA
                     DILGLDSPVSDEERTLIVLRPAGGDVYALAVDRIHDHEELVVKPAAPAVMATGLYAGT
                     TLADDGSPILLFDPAGLAEVGGVRLEAQERAARIAEGPAIAPAAKATEVILFRGLDGA
                     RRALRLSVVDRIEEVPADSIRPGAGQLRVQLGEAILPLAGIGGQELTADKVRLFRLND
                     GSHEVGYCFDEVIDLSVIDHDVIQSEQPGEISGVTLVAGEPAELVDAHWLFANHVGPA
                     AKPADEQPVCRIPADDPWMQNMLRPIVEAAGYRVVGEFDEVEADLVI"
BASE COUNT          484 a         1150 c         1115 g          463 t
ORIGIN      
        1 cggaacgcct cgcagccctt gaccctggtg gcggggtcgc gaaccggggg cgtcagggcg
       61 ccgctccgga tcagcgcctt cagccgctcg cgcgacaggg tcggcaccgc ggcggcaagc
      121 gcccggtcga ggcgccagcc ggcatggtcc ggacccagcc gcacgtcgat gatctgctgc
      181 cccccggcca ttgcgccaag catatgggcg cggctcgcgg cggttcaagc cagcgcccgc
      241 ttgcgctgcc acggccgttg ccgcatcacc gcgccgcgat gaacgccgtc gacctcgcca
      301 gcctgctctg ctcgcgcctg tgccacgatt tgatgtcgcc ggtcggggcg ctgaacaacg
      361 gcatcgaact gatggccgac gaaaccgacc ccgagatgct cgacaagtgc ctcgaattgc
      421 ttggggaaag cgcccgggca tcggccaaca agctcaaatt cttccgcctc gcgttcggcg
      481 ccgccggcgg ctttggcgac agcattgaca cccatgaggc gcaggccgcg ctcgaaggcg
      541 tgttcgggcc cgagcggcgc atcgagctcg gctggatggt ccccggcgac catttgccca
      601 agggcgcggt caaattgctg ctcaacctgg cgctgctggc cggcgatgcg ctggtccgcg
      661 gcggccggct ggacgtcggc gccgaatcga ccgacgagct cctggagctg gtgatccgcg
      721 ccgaaggccc gcggatcctg ctcgacccga cgcttcgggc aacgctgctc aacggcgagg
      781 cgaatgggac ggtcgagccg cgtgccgccg gcgcctggct cgcgcacagc ctcgccgccg
      841 aggccggcgg ctcgatccgc ctgtccgacc ccgccgacgc ggtgctgatg atgggtgcga
      901 cccttccccg acagcgatag ctgcgcgaca ggcccctcga tttcgcccgg gacagggata
      961 acgcagcgtt aacacctgcc cggcataagc gtccccgaac agcggttggg ggcccacagc
     1021 ggcgatggac gatctgattg ccgatttcgt agcggaatgc cgggagatgc tcgaatcgct
     1081 gggcggcgag atcgtcgcct gggaagcggc gcccgacgac cgcgcgcgcc tcgattccat
     1141 cttccgcttc gtccacaccg tcaaaggcaa ttgcggcttt ttcgaattcc cgcggctgga
     1201 agcgctcagc cacgcggccg aggacgcgct cgccgacgtc cgcgccggcc gccgccaggc
     1261 cgatggcgcg ctggtcagcg cggtgctcgc gatcatcgac cggatcggcg agatggtcgc
     1321 cgcgatcgac tccggctccg atatgccggc gggcgacgac agcgcgctga tcgcggcgct
     1381 cgagcccggc accgacggcg ccgtcgctcc ggtcgccgct acggtcgccg aggcgcaagg
     1441 caagatcgcc gctccgcgca ccatccgcct gtcggtcgag ctgctcgacc gggtgatgag
     1501 cggcgtttcc gacatggtgc tggcgcgcaa cgagctcgcc cgccggctcc gcgaatcccc
     1561 gaccgacgtg ccggtcgacg gcgccttcga gcgcctgtcg tcgatcatcg ccgagatgcg
     1621 cgacgcgatc acccgcaccc gcatgcagcg gatcgagaat ctgttcgtcg gcctgccgcg
     1681 catggtccgc gacctgtcgg ccgagctcgg caagcaggtg ctggtcgata tcgagggcgg
     1741 cgacgtcgag ctcgatcgcg agatgatcga gatgatccgc gatccgctga cccacatcat
     1801 ccgcaacgcc gtcgaccacg gcatcgagac tcccgccgag cggctgaagg ccggcaagcg
     1861 cgagatcggc atcctgtgcg tttccgcgcg ccagtcgggc aaccagatcc tgatcgacat
     1921 ccaggacgac ggccgcggca tcgacggcaa gaagctggtc gaaaaggcca tcgccgccgg
     1981 cacattgacc aaggccgccg ccgccgaaat gagcccgcgc gagcagctgg cgctgatctt
     2041 cgaggccggg ctgtcgaccg ccagctcggt caccgccatc tcgggccgcg gcgtcggcat
     2101 ggacgtcgtc cgctccaacg tcgagcgcat cggcgggctg gtcgaggtcg acagccgccc
     2161 gggcgtcggc acgcgcatga cgctgcgtgt gccgctgacg ctgaccatca tcccggccct
     2221 gacggtgtcg atcggcagcc agcatttcgc gattccgcgc tcggcgatcg atgagatcgt
     2281 ccgcgccaac ggcgaatcgg tgaagcttga gcgggtcggc gggtccggga tcgcgaccat
     2341 ccgcggccgc cgggtgccgc agatcgtgct cgccgacatc ctcggcctcg acagcccggt
     2401 cagcgacgag gagcgcacct tgatcgtgct ccggccggcc ggcggcgacg tctacgccct
     2461 cgcggtcgac cggatccacg accatgagga actggtggtg aagccggccg ccccggcggt
     2521 gatggcgacc ggcctgtacg cgggcacgac ccttgccgac gacggcagcc cgatcctgct
     2581 gttcgacccg gccgggctgg ccgaggtcgg cggcgtcagg ctcgaggcgc aggagcgcgc
     2641 cgcccggatc gccgaggggc cggcgatcgc gccggccgca aaagccaccg aggtgatcct
     2701 gttccgcggc ctcgacggcg cccggcgcgc gcttcgcctg tcggtcgtcg accggatcga
     2761 ggaagtgccc gcggactcga tccggcctgg cgccggccag cttcgggtgc agctcggcga
     2821 ggcgatcctt ccgctcgccg ggatcggcgg ccaggagctc accgctgaca aggtgcgcct
     2881 attccgcctg aacgacggca gccacgaggt cggctattgc ttcgacgagg tgatcgacct
     2941 gtcggtcatc gaccacgacg tcatccagtc cgagcagccg ggcgaaatca gcggcgttac
     3001 cctggtcgcc ggcgagccgg ccgaactggt cgacgcgcac tggctgttcg ccaaccatgt
     3061 cggcccggcc gcgaaaccgg ccgacgagca gccggtctgc cggatcccgg ccgacgaccc
     3121 gtggatgcag aacatgctgc gcccgatcgt cgaggccgcc ggctaccggg tggtcggcga
     3181 attcgacgag gttgaggccg atttggtgat cg
//
LOCUS       DASTZO010000044         4607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_239138,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000044 DASTZO010000000
VERSION     DASTZO010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4607
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239138"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VFU20_01130"
     CDS             <1..320
                     /locus_tag="VFU20_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU5285095.1"
                     /translation="DDPLLPARSFDRIFLVHMYHEVQSPYAFLWHLREGLKPDGLVVV
                     VELDRPTKRHGISPALLTCEFAAVGMRRVHVTTLTGGDAYLAMFGIGAPRPRPADIRP
                     CKG"
     gene            complement(340..744)
                     /locus_tag="VFU20_01135"
     CDS             complement(340..744)
                     /locus_tag="VFU20_01135"
                     /inference="COORDINATES: protein motif:HMM:NF015935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HEU5285096.1"
                     /translation="MRYQRAEFPLKLDGLDAARRFFAGCFADADPMSESLWVAHVDQQ
                     ARCVHLSRHAGDEEGADFPLRSIIADAAAHGSAGIVLAHNHPSGDPAPSDADCRATRR
                     LASAAEALDCAVLDHLVFAGTECTSLRRMGLL"
     gene            876..1670
                     /locus_tag="VFU20_01140"
     CDS             876..1670
                     /locus_tag="VFU20_01140"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEU5285097.1"
                     /translation="MRQYLDLMRLILETGVEQMDRTETGTLTHFGAQMRFDLADGFPL
                     LTTKKLHLRSIIVELLWFLRGDTNIRWLKERNVSIWDEWADANGDLGPVYGKQWRDWE
                     AADGRHIDQIRQLIELIKREPSSRRQIVTAWNPGEIDRMALAPCHCLFQTQAANGRLN
                     LQLYQRSADFFLGVPFNIASYALLTHMLAKECGLEPGVFVWTGGDCHLYSNHLEQARL
                     QLSREPRPLPRLVLRDRGQAIDGYEPGDFEFQAYDPHPHIAAPVAV"
     gene            1769..1894
                     /locus_tag="VFU20_01145"
     CDS             1769..1894
                     /locus_tag="VFU20_01145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285098.1"
                     /translation="MFRKLMTIVLIGGSVAVAACNTVRGAAADVNSVANEVDNNT"
     gene            complement(1996..>4607)
                     /locus_tag="VFU20_01150"
     CDS             complement(1996..>4607)
                     /locus_tag="VFU20_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU5285099.1"
                     /translation="VPQTDEQGNGYMKRFETTPGRMLLGETLPKSHKVPFETVNRLLT
                     KKEIGDVIDIVYRHTGQKETVLFADAIMQLGFRHAFAAGISFGKDDMVIPGSKVGLVD
                     DTRGLVKDYEQQYQDGLITQQEKYNKVIDAWSRCGDQVATAMMAEISATPKGEDGREA
                     PVNSIYMMAHSGARGSQAQIKQLAGMRGLMAKPSGEIIETPIISNFKEGLTVLEYFNS
                     THGARKGLADTALKTANSGYLTRRLVDVSQDAVIIEEDCGTDRALEMRAIVQGGAVIA
                     SLSDRVLGRTTAEDIVDPKTSDVIIPEGSLLDEAMVAKIEEIGLQGVRIRSPLICASK
                     QGVCAKCYGRDLARGTPVNIGEAVGVIAAQSIGEPGTQLTMRTFHIGGAAQLNEQSNL
                     EAPVDGTIEFRDMPMIIDPRGRHVSLSRSGEIAILDMDGRELSTHRVPFGAHLLCESG
                     HIVSRGDRVAEWDPSFSPVITEKAGTVAYQDLIENRTLTEQVDESTGITQRVVTEDMA
                     KSKKDTLHPRLTLTGGKAKEAGVYRLASGAIIAVEDGQAVEAGEVLARLPREAARTRD
                     ITGGLPRVAELFEARKPKENAIIAKVSGKVSFMKDYKAKRKIAIIPDDGSEPVEYLVP
                     KSKVIDVQEGDYVKRGDNLVGGSPDPHDILEVLGVEALAEYLVSEIQEVYRLQGVKIN
                     DKHIEVIVRQMLQKVEITDGGDTTLLAGEQVDRDEMDEANSRLEKKQRKAEGKPVLLG
                     ITKASLQTRSFISAASFQETTRVLTEAAVQGKIDTLNGLKENVIVGRLIPAGTGAGMN
                     RLRVAASSRDTALRAAQRKLQESLILPNSAAEEHAAELAQGPEAAVNADPLAEVAPSG
                     NGLDADAGEYLKD"
BASE COUNT          690 a         1583 c         1513 g          821 t
ORIGIN      
        1 ccgacgaccc gctgcttccc gcccgctcgt tcgaccgcat cttcctggtc cacatgtacc
       61 atgaggtgca gtcgccctac gcgttcctgt ggcatctgcg cgaagggctc aagcccgacg
      121 gcctggtggt ggtggtcgag ctcgaccggc cgaccaagcg ccacgggatt tcgcccgccc
      181 tgctgacctg cgagttcgcc gcggtcggga tgcggcgcgt gcacgtcacg accctgaccg
      241 gcggcgacgc ctatctggcg atgttcggga tcggggcgcc gcggccgcgc ccggccgaca
      301 tccggccgtg caaggggtaa gcgcgctcgg cggctgaggc tacagcaggc ccatccggcg
      361 caggctggtg cactcggtgc cggcgaacac cagatgatcg agaaccgcgc agtcgagcgc
      421 ttccgccgcc gacgccagcc ggcgggtcgc tcggcaatcg gcgtcgctcg gcgccgggtc
      481 gccgctcggg tgattgtgcg cgagcacgat cccggcgctg ccgtgggccg ccgcgtcggc
      541 gatgatcgag cgcaggggga aatcggctcc ttcctcatcg ccggcgtgac ggctcaggtg
      601 gacgcagcgc gcctgctggt cgacatgggc gacccacagg ctttcggaca tcggatctgc
      661 gtcggcgaag caaccggcga agaagcggcg cgcggcgtcg agcccatcca gtttcagcgg
      721 aaattcggca cgctgatatc gcatcgggcg acattaggtg cggcggtggc gggcggccac
      781 ttcaaccgcg tccgccccgg agcgaaaaac cgtccgaagc ggaccaaacc acccctcttg
      841 gtggccgacc gcgcagcgta tagggatagc gcccgatgcg gcaatatctc gacctgatgc
      901 ggctgatcct cgagacgggc gtcgagcaga tggaccgcac cgagaccggc accctgaccc
      961 acttcggggc gcagatgcgc ttcgacctcg ccgacggctt cccgctgctg acgaccaaga
     1021 agctgcacct gcgctcgatc atcgtcgaat tgctgtggtt ccttcgcggc gacaccaaca
     1081 tccgctggct caaggagcgc aacgtgagca tctgggacga gtgggccgac gccaacggcg
     1141 acctcggccc ggtctacggc aagcagtggc gcgactggga agccgccgac ggccgccaca
     1201 tcgaccagat ccgccagctg atcgagctga tcaagcgcga gccctcgtcg cgccgccaga
     1261 tcgtcaccgc ctggaacccg ggcgagatcg accggatggc gctcgccccg tgccactgcc
     1321 tgttccagac ccaggcggcg aatgggcggc tcaacctcca gctctaccag cgcagcgccg
     1381 atttcttcct cggggtaccg ttcaacatcg ccagctacgc gctcctgact cacatgctag
     1441 ccaaggagtg cgggctcgag cccggagtgt tcgtctggac cggcggcgac tgccaccttt
     1501 actccaacca tctcgagcag gcccggctcc agctcagccg cgagccgcgg ccgctgcccc
     1561 ggctggtgct cagggaccgc gggcaggcga tcgacggtta cgagcccggg gatttcgagt
     1621 tccaggccta cgacccgcac ccccacatcg ccgccccggt cgcggtctag gcggtggcac
     1681 ccacaagccg tgagaaaatt gcggtagcgc aatggagcgc ttttgtgcat ttgcgcgttc
     1741 atcgggtgca aggacaggag aaaacgacat gtttcgcaag ctgatgacca tcgtattgat
     1801 cggcgggtcg gtcgcggttg ccgcgtgcaa caccgtccgc ggcgccgctg cagacgtcaa
     1861 ttcggtcgcc aacgaggtcg acaacaacac ctgatcccgt ccgcttattg agtttgcaaa
     1921 cgacaaaagc cccgccggat cgctccggcg gggctttctt gtcggcgatc gaagccttgt
     1981 cggcgaacga aggcgtcagt ccttcagata ctcgcccgca tcggcgtcga ggccgttgcc
     2041 cgacggcgcg acctcggcca gcggatcggc attgaccgcc gcttccggcc cctgcgccag
     2101 ctcggccgca tgctcctcgg ccgcgctgtt cggcaggatc agcgactcct gcagcttgcg
     2161 ctgggccgcc ctcagcgcgg tgtcgcggct cgacgcggcg acccgcaggc gattcatgcc
     2221 ggcgccggtc ccggccggga tcagccggcc gacgatgaca ttctccttga gcccgttcag
     2281 agtgtcgatc ttgccctgga ccgcggcctc ggtgagcacc cgggtcgtct cctggaacga
     2341 tgccgccgag atgaagctgc gggtctgcag cgacgccttg gtgatcccga gcaggaccgg
     2401 tttgccctcg gccttccgct gcttcttctc gaggcgcgaa ttggcctcgt ccatctcgtc
     2461 gcggtcgacc tgctcgccgg cgagcagggt ggtgtcgccg ccgtcggtga tctcgacctt
     2521 ctgaagcatc tggcggacga tcacctcgat atgcttgtcg ttgatcttga cgccctggag
     2581 ccggtagacc tcctggatct cgctgacgag atattcggcc agcgcctcga cccccagcac
     2641 ctcgaggatg tcgtgcggat cgggtgagcc gccgacgaga ttgtcgccgc gcttcacata
     2701 gtcgccttcc tggacgtcga tcaccttcga cttgggaacc agatactcga ccggctccga
     2761 gccgtcgtcg gggatgatcg cgatcttacg cttggccttg tagtccttca tgaagctgac
     2821 cttgcccgag accttggcga tgatcgcatt ctccttgggc ttgcgggcct cgaacaactc
     2881 ggcaacccgc ggcagaccgc cggtgatgtc gcgtgtcctc gcggcctcgc gcggcaagcg
     2941 ggcgagcacc tcgccggcct cgaccgcctg gccgtcctcg accgcgatga tcgcccccga
     3001 agcgaggcgg tagacgcccg cttccttggc cttgcccccg gtcagggtga gccgcgggtg
     3061 gagcgtgtcc ttcttcgact tcgccatgtc ctcggtcacc acgcgctggg tgatcccggt
     3121 cgactcgtcg acctgctcgg tgagcgtgcg gttctcgatc aggtcctggt aggcgaccgt
     3181 gcccgctttc tcggtgatca ccgggctgaa cgaggggtcc cactcggcga cccggtcgcc
     3241 gcggctgacg atgtggccgc tctcgcacag caggtgcgct ccgaacggca cccggtgggt
     3301 cgacagctcg cgcccgtcca tgtcgaggat cgcgatctcg cccgagcgcg acagcgacac
     3361 gtgccggccg cgcgggtcga tgatcatcgg catgtcgcgg aactcgatcg tgccgtcgac
     3421 cggcgcctcg aggttcgact gctcgttgag ctgagcggcg ccgccgatgt ggaaggtccg
     3481 catcgtcagc tgggtgcccg gctcgccgat cgactgggcg gcgatcactc cgaccgcctc
     3541 gccgatgttg accggcgttc cgcgcgcgag gtcgcgcccg tagcatttgg cgcacacgcc
     3601 ctgcttggac gcgcagatca gcggcgagcg gatgcgcacg ccctggagcc cgatctcctc
     3661 gatcttggcg accatcgcct cgtcgagcag cgagccctcg gggatgatga cgtcgctggt
     3721 cttgggatcg acgatgtcct cggcggtggt ccggccgagc acccggtcgg acagcgaggc
     3781 gatcaccgcg ccgccctgga cgatggcgcg catctcgagc gcccggtcgg tgccgcaatc
     3841 ctcctcgatg atcaccgcgt cctggctgac gtcgaccagc cgccgcgtca gatagcccga
     3901 gttggcggtc ttcagcgcgg tgtcggcgag gcccttgcgg gcgccgtggg tcgagttgaa
     3961 atattcgagg acggtcagcc cctccttgaa gttggagatg atcggcgtct cgatgatctc
     4021 gcccgacggc ttggccatca ggccgcgcat gccggcgagc tgcttgatct gcgcttggct
     4081 gccgcgcgcg cccgagtggg ccatcatgta gatcgagttg accggcgcct cgcggccgtc
     4141 ttcgcccttc ggagtggccg agatttccgc catcatcgcg gtcgccacct ggtcgccgca
     4201 gcgggaccag gcgtcgatca ccttgttgta cttttcctgc tgggtgatca gtccgtcctg
     4261 atactgctgc tcgtaatcct tgacgagccc gcgggtgtcg tcgaccaagc ctaccttcga
     4321 acccggaatg accatgtcgt ccttgccgaa cgaaatgccg gcggcgaagg cgtggcggaa
     4381 gccgagctgc atgatcgcgt cggcgaacag caccgtctcc ttctggccgg tgtggcgata
     4441 gacgatgtcg atgacgtcgc cgatctcctt cttggtgaga aggcggttga cggtctcgaa
     4501 cggcactttg tggctcttcg gaagtgtctc gccgagcagc attcggcccg gggtcgtctc
     4561 gaaccgcttc atatagccgt tgccctgctc gtccgtctgc gggacgc
//
LOCUS       DASTZO010000045         8016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_865821,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000045 DASTZO010000000
VERSION     DASTZO010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865821"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFU20_01155"
     CDS             <1..190
                     /locus_tag="VFU20_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285100.1"
                     /translation="EGAELRDVETARRFATDSARVLMCETMKERGRINLNHRIDVEDE
                     QGTVLTSVFFGDAVKIDD"
     gene            323..1324
                     /gene="argC"
                     /locus_tag="VFU20_01160"
     CDS             323..1324
                     /gene="argC"
                     /locus_tag="VFU20_01160"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU5285101.1"
                     /translation="MVRTAILGASGYVGAELLRLCAAHPHIRAARLFGDSRAGEPLAA
                     VHPQLALDYPGSRVEAYDPAALDGIDLVFAALPHGRSQDIAPAILERGIPLVDLGADF
                     RLNDAGAYARWYGEEHRSPGRLASFAYGLPEMHREAIVSAKAVAAPGCYPTAAILALK
                     PLLGLIEPGSIIVDAASGVSGAGGSPSAATHFSTVAENFTAYGLLNHRHTAELEMELG
                     EGVLFTPHLTPMNRGILATCYGLAKGPCDPLEALAEAYAGEPFIQVSEQPPSTKWTLG
                     SNAAHLTARFDQRTGRVLALAALDNLGKGAAGQMVQCANLMLGLDEAAGLSRAGVCP"
     gene            1321..2481
                     /gene="argJ"
                     /locus_tag="VFU20_01165"
     CDS             1321..2481
                     /gene="argJ"
                     /locus_tag="VFU20_01165"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEU5285102.1"
                     /translation="MSITAAPGFVAAGCHAGIKRRRFDLALLATEDGRPVTAAAVFTQ
                     NKFAAPPVELDRARLAENGGEVAAVIVNSGNANAGTGAQGHADAEAMAAAAARALGID
                     SQHVLVCSTGIIGKHLPIDAILDATPKLTARLSRKGADDAAKAILTTDSKPKQALVRG
                     SNFTVGGMAKGCGMIAPNMATMLAFLTTDAKLPQPMLQQLLREACDATFNTLNVDGAT
                     STNDSAMLLANGRAGAVDPAEFADALHKLCEDLTWQMALDAEGMTKIAVVRVSGAASA
                     SEARRAAKAIAENNLVKCSWYGADAYWGRILAAAGSADIAFETQRTSVAYGDVVVARG
                     GAEVPHDAAAVAAHMAGERIDIHVALGLGSGSATMIGVDLGPGYIKENSATS"
     gene            2478..3341
                     /gene="argB"
                     /locus_tag="VFU20_01170"
     CDS             2478..3341
                     /gene="argB"
                     /locus_tag="VFU20_01170"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEU5285103.1"
                     /translation="MISPAVSAELLVQALPYIQRFNGKAVVVKLGGAAIDEALDRALA
                     QDVLLLRSVGVRCVLVHGGGPQVDELMRRMGKEPEFRDGLRVTDAETLDIVRMVLAGK
                     VNRDLVATINREALDDPVAVGVSGEDAGLLTAVAGDPALGFVGEVAHVRASVLDRLLD
                     EGLAPVVSTIGSDSAGQPHNINADEAARAIAVAMKAEKIVYLTAAPGLLDDPADEGSL
                     VHQITSGQLRERIEHESVSAGMIPKLRACADAVDGGVGTAHIIDGRVAHALLIELLTD
                     AGIGTMVRRGE"
     gene            3338..4219
                     /locus_tag="VFU20_01175"
     CDS             3338..4219
                     /locus_tag="VFU20_01175"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF001986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU5285104.1"
                     /translation="MSGLLTIADLGKDDLLHILELSERDDLGRPLAGQGVALYFQKPS
                     ARTRNSMEMAVVQLGGHPVHIRNEEIDPGVRESVADVARTLACYHALIAARVKDHRVL
                     AEMAQATATPVVNMLSDTHHPLQALADLLTIRQLLGRLEGARIAFIGDGANNVCRSLA
                     QGCALAGAELRIASPDGHGLPAAELERLDGVEQLSDPAEAADGAQIVYTDVWASMGNE
                     AEAEQRAAVFAPYQVDQALMARSANGWFLHCLPAHRGAEVIAGVIDGPRSAVWRQAEN
                     RMHSARGAIAWLVGSGR"
     gene            4216..4683
                     /gene="argR"
                     /locus_tag="VFU20_01180"
     CDS             4216..4683
                     /gene="argR"
                     /locus_tag="VFU20_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU5285105.1"
                     /translation="MTDARSKRQRAIVDLLREAPVRSQDEIASRLVTLGYAVTQATVS
                     RDLDQIGAVKVRRGGALGYSLPDQLGDRDWAAKRLERIFGEWAQSIETSGSMVVVRTP
                     PGSAHVVGLALDQAKLPEVAGTIAGDDTLFVAVRSGIAVEPFAQRLRDLIRIA"
     gene            complement(4680..6086)
                     /locus_tag="VFU20_01185"
     CDS             complement(4680..6086)
                     /locus_tag="VFU20_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5285106.1"
                     /translation="MKPDPLDRQAKLIAAVRERLGPRAAITDPQDVEPWLTDWRGRVH
                     GRAAAILAPGSTEEVAAIVGLAAEHRVPLVPQGGNTSMVAGATPPEDGSALILSLRRM
                     NALRSISAETNLAVAEAGMILADLHERAAEIGRRFPLTLGARGSCTIGGLVATNAGGT
                     QVLRFGTMRSLVAGLEAVLPDGSVHHGLTALKKDNRGYSLDHLLIGSEGTLGIVTAAA
                     LKLVPAVAARAVAWGGLADPQAALDLLRAMQAQSDIVEGFEIVPGDSLDLVLKHIPGT
                     RSPLAGRHRWHVLVEATSPDSSEPPGARLEAMLGEALEAGLVEDAAIASSEAQAEAFW
                     RIRDSISEAERAAGGSIAHDISVPVADMPRFMTAAAAELERAFPGVVASGFGHLGDGN
                     VHFHVRAKGHPPAAISHMVHDLVTAAGGSISAEHGIGQVKLEEFERLAPPGRIAALKA
                     IKRALDPQGLMNPGKLVR"
     gene            6173..7033
                     /locus_tag="VFU20_01190"
     CDS             6173..7033
                     /locus_tag="VFU20_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5285107.1"
                     /translation="MNPLPVALGAAAVALIAYGGRSALRRTLRRARDDHARRPALWDR
                     FYALDWGETTTNNYGFAPAEGDHPQRFQHQMYRELLRLLKEKRSLEPGLKLLEVSCGR
                     GGGLGALLAAAPGVFDATGLDVAASAVAFCRSTYGENDRLRFVEGSALDLPFPDSSFD
                     VLLNVEASNDYGDRPRFFAEVARVLKPGGVFLYTDSFRAAQAEQMKRELAAAGFEAEF
                     TDVTANVAEACRLDSPRRRELIARQAPLVGRLMFRRQLENYAGVEGSGKYRQFAERRR
                     AYLMTAAVKA"
     gene            7030..7515
                     /locus_tag="VFU20_01195"
     CDS             7030..7515
                     /locus_tag="VFU20_01195"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEU5285108.1"
                     /translation="MTRPPISLVVARASNGVIGKGGGLPWHIPADLRRFKALTMGTVL
                     VMGRKTFDSLPKVLPGRRHIVITRDPAWRADGIEVAHSIGEALTKAGEEPVSVIGGAD
                     IFQLFLPLADRIELTEVLADVDGDTFLDDPRSAGEWREVAREDHAGEEPPFRFVTLER
                     A"
     gene            complement(7512..>8016)
                     /locus_tag="VFU20_01200"
     CDS             complement(7512..>8016)
                     /locus_tag="VFU20_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEU5285109.1"
                     /translation="ALAPPAIAKAEVVSASAHGFEVRHIVSTVIPQPAAFEAFGQISN
                     WWGKDHTYSGDSANLSLSLSPGGCFCERLPTGGGVEHMHVAYVVPGERVVLTGSLGPL
                     LYEATAGVMDVKVDRIAGGSRLTMNYRAAGFANGGADKLAPLVDQVLGEQIARFRKFA
                     AAMPQTR"
BASE COUNT         1194 a         2859 c         2828 g         1135 t
ORIGIN      
        1 ggagggcgcc gagcttcgcg acgtcgagac ggcgcgcagg ttcgccaccg actcggcacg
       61 agtgctgatg tgcgaaacga tgaaagaacg cggccggatc aacctcaacc atcgaatcga
      121 cgtcgaggac gaacagggca cggtgctgac aagcgtgttc tttggcgacg cagtgaagat
      181 cgacgactag ctcaaccggc ctgccgaccc ccggcgatct tcgcggtcga tgccctactg
      241 gccgctagac cagggacgcg ggtccagctg cataatcatt ttatgtattg aataattatg
      301 cacatccgtg catgggcgcc cgatggttcg aacggcgatc ctcggtgcgt cgggttatgt
      361 cggcgcggaa ctgctgcggc tttgcgcggc ccatccgcac atccgcgcgg cgcggctgtt
      421 cggcgacagc cgcgccggcg agccgctcgc cgccgttcat ccgcagctgg cgctcgatta
      481 tccgggctcc cgggtcgagg cctacgaccc ggccgcgctc gacgggatcg acctggtctt
      541 cgcggccttg ccccacggcc ggtcgcagga catcgccccg gcgatcctcg agcggggcat
      601 cccgctggtc gatctcggcg ccgacttccg gctgaacgac gccggcgcct atgcgcgctg
      661 gtacggcgag gagcatcgct cgcccgggcg gctcgcaagc tttgcctacg gccttcccga
      721 gatgcaccgc gaggcgatcg tctcggccaa ggcggtcgcg gcgcccggct gctacccgac
      781 cgccgcgatc ctcgcgttga agccgctgct cggcttgatc gagcccggca gcatcatcgt
      841 cgacgcggcc tcgggggtca gcggcgccgg cggctcgccc agcgcggcga cgcatttttc
      901 gaccgtcgcc gagaatttca ccgcctacgg gctgctcaac caccgccaca ccgcggagct
      961 ggagatggag ctaggcgagg gcgtgctgtt cacgccgcac ctgacgccga tgaaccgcgg
     1021 catcctcgcg acctgctacg ggctcgccaa ggggccttgc gacccgctcg aggcgctcgc
     1081 cgaggcgtat gccggcgagc cgttcatcca ggtttccgag cagccgccgt cgaccaaatg
     1141 gacgctgggc agcaacgccg cgcacctgac cgcccggttc gaccagcgca ccggccgggt
     1201 gctggccctc gccgcgctcg acaatctcgg caagggggcg gcggggcaga tggtccagtg
     1261 cgcgaacctg atgctcgggc tcgacgaggc cgccgggctg agccgcgcgg gagtgtgccc
     1321 gtgagcatca cagccgcccc cggattcgtc gccgccggct gccacgccgg gatcaagcgc
     1381 cgccgcttcg acctcgcgtt gctcgccacc gaggacggcc ggccggtgac cgcggctgcg
     1441 gtgttcacgc aaaacaagtt cgccgctccg ccggtcgagc tcgaccgggc gcgcctcgcc
     1501 gagaacggcg gcgaggtcgc ggcggtgatc gtcaattcgg gcaacgccaa cgccggcacg
     1561 ggcgcgcaag gccatgccga tgccgaggcg atggccgcgg ccgccgcgcg ggcactcgga
     1621 atcgactctc agcacgtgct cgtctgctcg accgggatca tcggcaaaca cctgccgatc
     1681 gacgcgatcc tcgacgccac cccgaagctc accgccaggc tgtcgcgcaa gggagccgac
     1741 gacgccgcca aggcgatcct gaccaccgac agcaagccca agcaggcgct ggtccgaggc
     1801 agcaatttca ccgtcggcgg gatggccaag ggctgcggga tgatcgcgcc caacatggcg
     1861 acgatgctcg ccttcctgac caccgacgcg aagctgccgc agccgatgct gcagcagctg
     1921 ttgcgcgagg cgtgcgacgc gaccttcaac acattgaacg tcgacggcgc gacctcgacc
     1981 aacgacagcg cgatgctgct cgccaacgga cgcgccgggg cggtggatcc ggccgagttc
     2041 gccgacgcct tgcacaagct gtgcgaggat ttgacctggc agatggcgct cgacgccgaa
     2101 ggaatgacca agatcgccgt cgtccgggtc agcggcgccg ccagcgcgag cgaggcgcgc
     2161 cgggcggcca aggcgatcgc cgagaacaat ctcgtcaagt gcagctggta cggcgccgac
     2221 gcgtactggg gccggatcct cgccgccgcc ggctcggccg acatcgcctt cgagacccag
     2281 cggacgagcg tcgcctacgg cgatgtggtc gtcgcccgcg gcggcgccga ggtcccgcat
     2341 gatgccgcgg ccgtcgccgc gcacatggcc ggcgagcgca tcgacatcca cgtcgcgctc
     2401 ggcctcggct cgggcagcgc gacgatgatc ggcgtcgatc tcggccctgg ctacatcaaa
     2461 gagaatagcg cgacgtcatg atctcgcccg ccgtctccgc cgagctgctg gtccaggcgc
     2521 tcccctatat ccagcgcttc aacggcaagg ccgtcgtcgt caagctgggc ggagcggcga
     2581 tcgacgaggc gctcgaccgg gcgctcgccc aggacgttct gctgctgcgc agcgtcggcg
     2641 tccgctgcgt gctggtccac ggcggcggcc cgcaggtcga tgagctgatg cggcggatgg
     2701 gcaaggagcc cgagttccgc gacggccttc gggtgaccga cgccgagacg ctcgacatcg
     2761 tccgcatggt gctcgccggc aaggtcaacc gcgacctcgt cgcgaccatc aaccgcgagg
     2821 cattggacga cccggtcgcg gtcggcgtgt cgggcgagga cgcggggctt ctgacggcgg
     2881 tggcgggcga cccggcgctg ggcttcgtcg gcgaggtcgc gcacgtgcgc gcgtcggtgc
     2941 tcgaccggtt gctcgacgaa ggcctggccc cggtggtctc gacgatcggc tcggactcag
     3001 ccggccagcc gcacaacatc aacgccgacg aggccgcccg ggcgatcgcg gtggcgatga
     3061 aggccgagaa gatcgtctat ctgaccgccg ctccgggcct gctcgacgat cccgccgacg
     3121 agggcagcct cgtccaccag atcacgtcgg gccagctgcg cgagcggatc gagcacgaga
     3181 gcgtcagcgc cgggatgatc cccaagctgc gcgcctgcgc cgacgcggtc gacggcggcg
     3241 tcggcaccgc ccacatcatc gacgggcggg tggcgcacgc gcttctgatc gagctcctga
     3301 ccgacgccgg gatcggcacc atggtccgga ggggcgaatg agcggcctgc tgacgatcgc
     3361 cgacctcggc aaggacgatc tgctgcatat cctcgagctg tccgaacgcg acgacctcgg
     3421 ccggccgctc gccggacagg gcgtcgcgct ctatttccag aagccgagcg cccgcacccg
     3481 caactcgatg gagatggcgg tcgtgcagct cggcgggcac ccggtccaca tccgcaacga
     3541 ggagatcgac cccggggttc gcgaaagcgt cgccgacgtc gcccggacgc tcgcctgcta
     3601 ccacgcgctg atcgccgccc gggtcaagga ccaccgggtg ctggccgaga tggcgcaggc
     3661 gaccgccacg ccggtggtca acatgctgtc cgacacgcac cacccgctgc aggcgctcgc
     3721 cgacctgctg accatccgcc agcttctcgg gcggctcgag ggcgcgcgca tcgccttcat
     3781 cggcgatggc gccaacaacg tctgccggtc gctggcgcaa ggctgcgcgc tcgctggcgc
     3841 cgagcttcgc atcgccagcc ccgacggcca cggattgccg gcggccgagc tcgaacggct
     3901 cgacggagtc gagcaattgt ccgatcccgc cgaagcggcc gacggcgcgc agatcgtcta
     3961 taccgacgtc tgggcgtcga tgggcaacga ggccgaagcc gagcagcggg cagcggtttt
     4021 cgcgccttat caagtcgacc aggcactgat ggcgcgctcg gccaacggct ggttcctgca
     4081 ctgcctgccc gcgcaccgcg gcgcggaggt catcgccggc gtgatcgacg gcccgcgaag
     4141 cgcggtctgg cgccaggcgg aaaaccggat gcacagcgcg cgcggggcga tcgcctggct
     4201 ggtggggagc ggccgatgac cgacgccagg agcaagcggc agcgcgcgat cgtcgatttg
     4261 ttgcgcgaag cgccggtgcg cagccaggac gagatcgcct cgcggctcgt aacgctcggc
     4321 tacgcggtca cccaggcgac ggtgtcgcgc gacctcgacc agatcggcgc ggtcaaggtt
     4381 cggcgcggcg gggcgcttgg ctattcgctg cccgaccagc tcggcgaccg cgattgggcg
     4441 gccaagcggc tcgaacggat tttcggcgaa tgggctcagt cgatcgagac ttcgggcagc
     4501 atggtcgtcg tccgcacgcc gcccgggtcg gcccacgtcg tcggcctggc gctcgaccag
     4561 gccaagctgc ccgaagtggc cggaacgatc gccggcgacg acaccctgtt cgtcgcggtc
     4621 cgcagcggca ttgccgtcga gccgttcgcc cagcggctgc gcgacctgat ccggatcgcc
     4681 tagcggacca gcttgcccgg gttcatcagc ccttgggggt cgagcgcgcg cttgatcgcc
     4741 ttcagcgcgg cgatccggcc cgggggcgcg agccgctcga actcctccag tttcacctgg
     4801 ccgatgccgt gctcggcgga gatcgagccg ccggccgcgg tgacgaggtc gtggaccatg
     4861 tggctgatcg ccgccggggg gtggcccttc gcccgcacgt ggaaatgcac gttgccgtcg
     4921 cccaggtggc cgaagccgct ggcgacgacc cccgggaagg cgcgctcgag ctcggccgcg
     4981 gcggcggtca tgaagcgcgg catgtcggcg acggggaccg agatgtcgtg ggcgatcgag
     5041 ccgccggccg cccgctcggc ttcggaaatc gaatcgcgga tccgccagaa cgcctcggcc
     5101 tgggcctcgc tcgacgcgat cgccgcatcc tcgaccagcc ccgcctcgag cgcctcgccg
     5161 agcatcgctt cgagccgggc tccgggcggc tcggacgagt ccggcgaggt ggcctcgacc
     5221 agcacgtgcc agcggtgccg gccggcgagc gggctgcggg tgccggggat atgcttcaga
     5281 accaggtcga gcgagtcgcc gggcacgatc tcgaagcctt cgacgatgtc gctctgggcc
     5341 tgcatcgcgc gaagcaggtc gagcgccgcc tgcgggtcgg cgagcccgcc ccaggcgacc
     5401 gcccgcgccg cgaccgctgg caccagcttg agcgccgcgg cggtgacgat cccgagcgtg
     5461 ccctccgagc cgatcagcag atggtcgagg ctgtagccgc gattgtcctt cttgagcgcg
     5521 gtcaggccgt ggtggaccga gccgtcggga agcaccgctt ccagccccgc gaccagcgag
     5581 cgcatggtcc cgaaccggag cacttgggtg ccgccggcgt tggtcgcaac gagcccgccg
     5641 atggtgcagc tgccgcgcgc tccgagagtc agcgggaagc ggcggccgat ttcggccgcg
     5701 cgctcgtgca ggtcggcgag gatcatcccc gcctccgcca ccgccaaatt ggtctcggcg
     5761 ctgatcgagc gcagcgcgtt catccgccgc agcgagagga tcagggcgct gccgtcctcg
     5821 ggcggggttg cgccggcgac catcgacgtg ttgccgccct gcgggaccag cgggacgcga
     5881 tgctcggcgg cgaggccgac gatcgccgcg acctcttcgg tcgagcccgg cgcgaggatc
     5941 gctgcggccc gcccgtggac ccggccgcgc cagtcggtca gccacggctc gacgtcctgt
     6001 gggtcggtga tggctgcccg cggcccgagc ctctcccgga ctgcggcgat cagcttcgct
     6061 tgtcgatcaa ggggatcggg cttcattggc gaagcatagc ggcaatcggc gggcttggca
     6121 ttggccgccc gtgccgccaa tgcagcgcca gcctgtcggg agttgattga ggatgaaccc
     6181 attaccggtt gcgctcggtg cggcggcggt ggccctgatc gcctacggcg gccgctcggc
     6241 ccttcggcgc accctgcgcc gggcccgcga cgatcatgcg cggcgaccgg cgctgtggga
     6301 ccgcttctac gcgctcgact ggggcgagac gacgaccaac aattacggct tcgctccggc
     6361 cgagggcgac cacccgcagc gcttccagca ccagatgtac cgcgagctgc tgcggctctt
     6421 gaaggagaag cggtccctcg agccgggcct caagctgctc gaagtcagct gcggccgcgg
     6481 cggcgggctg ggcgccttgc tcgccgccgc cccgggcgtg ttcgacgcca ccgggctcga
     6541 tgtcgccgcg agcgcggtcg ccttctgccg ctcgacctac ggcgagaacg accggctgcg
     6601 cttcgtcgag ggcagcgcgc tcgacttgcc gttccccgac tcgagcttcg acgtcctcct
     6661 caacgtcgag gcgtcgaacg attacggcga ccggccgcgc ttcttcgccg aagtggcgcg
     6721 ggtgctgaag ccgggcggcg tgttcctcta caccgacagc ttccgcgccg cccaggccga
     6781 gcagatgaag cgcgagctcg ccgccgccgg gttcgaggcc gagttcaccg acgtcaccgc
     6841 caacgtcgcc gaggcgtgcc ggcttgacag cccgcgccgc cgcgagctga tcgcccgcca
     6901 ggcgccgctg gtcgggcggc tgatgttccg ccgccagctc gagaattacg ccggggtcga
     6961 gggcagcggc aaataccgcc agttcgcgga acggcgccgc gcctatctga tgaccgccgc
     7021 ggtcaaggca tgacgcggcc gccgatctcc ctggtggtcg cccgggcgag caacggcgtg
     7081 atcggcaagg gcggcggcct tccgtggcac atcccggccg acctccggcg cttcaaggcg
     7141 ctgaccatgg gcacggtgct ggtgatgggg cgcaagacct tcgacagcct ccccaaggtg
     7201 cttccggggc ggcggcatat cgtcatcacc cgcgatcccg cctggcgggc cgacggaatc
     7261 gaggtcgccc acagcatcgg cgaggcgctg acaaaggccg gcgaggagcc ggtctcggtg
     7321 atcggcggcg ccgacatctt ccagctgttc ctgccgctcg ccgaccggat cgagctgacc
     7381 gaagtgctcg ccgacgtgga cggcgacacc ttcctcgacg atccgcgctc ggccggcgag
     7441 tggcgcgaag tggcgcgcga ggatcatgcg ggcgaggagc cgccgttccg cttcgtcacc
     7501 ctcgagcgcg cctagcgcgt ctggggcatg gccgcggcga atttgcggaa ccgcgctatc
     7561 tgctcgccca ggacttggtc gaccagcggg gcaagcttgt cggcgccgcc gttggcgaag
     7621 cccgcggcgc ggtagttcat ggtcagccgc gagccgccgg cgatcctatc gaccttgacg
     7681 tccatcaccc cggcggtcgc ctcgtacagc aaaggcccga gggagccggt cagaaccacc
     7741 cgctcgccgg ggacgacata ggcgacgtgc atatgctcga ccccgccgcc ggtcggcagc
     7801 cgctcgcaaa agcagccgcc cgggctgagc gacagtgaca ggttcgcgga atcgcccgag
     7861 tacgtgtggt ccttgcccca ccaattcgaa atctgcccga acgcctcgaa cgccgcgggc
     7921 tgcgggatga cggtcgaaac gatatgccgg acctcgaaac cgtgcgcgct cgccgagacg
     7981 acctcggcct tcgcgatggc cggcggcgcg agcgcg
//
LOCUS       DASTZO010000046         4461 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_605119,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000046 DASTZO010000000
VERSION     DASTZO010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605119"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            20..1012
                     /gene="murB"
                     /locus_tag="VFU20_01205"
     CDS             20..1012
                     /gene="murB"
                     /locus_tag="VFU20_01205"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU5285110.1"
                     /translation="MSGDKETTVRPEPVEGLILSTPAVRKGQSLDKLGTNGLEVNVRG
                     RLTKDAPLAPLVWFKSGGNAEWLFEPADADDLAEFLRGLDPDVPVMALGLGSNLIVRD
                     GGVPGVVVRLGKAFARIEQPDDTLLRCGGGASGILVSSTARDAGIAGLEFLRGIPGTV
                     GGFVRMNGGAYGREVKDILVSATLVLRSGEVEEWSLDRLGYSYRHSAVPDGSVVIDAT
                     FRGTRGDPARIGAEMDRIAAAREETQPIRSRTGGSTFKNPPGHKAWALIDAAGCRGMT
                     LGGAQVSEKHCNFLLNLGSATSSDIEALGEEVRRRVRARTNISLEWEIQRVGSE"
     gene            1023..1958
                     /locus_tag="VFU20_01210"
     CDS             1023..1958
                     /locus_tag="VFU20_01210"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU5285111.1"
                     /translation="MNKDLHVVVLMGGWSSEREVSLTSGRGVAQALRTRGWANVTEID
                     MDRNVAQLITAVRPDVVFNALHGTPGEDGTVQGMLDLMQIPYTHSGLETSVIAIDKEL
                     TKMVLVPHGIRMPPGRLVESESLYAADPMPRPYVLKPVNEGSSVGVAIVTDDGNTGNP
                     IGREVEGPWHHFDKLLAEPFVKGRELTVAVLGGEALAVTELKPVAGFYDYDAKYTDGL
                     TVHVCPAEIPEDIARATMDMAARAHRLLGCKGASRSDFRWDDEAGEAGIFLLEINTQP
                     GMTPLSLVPEQAKYCGIGYGELVERLIVEALGDEP"
     gene            1955..2863
                     /locus_tag="VFU20_01215"
     CDS             1955..2863
                     /locus_tag="VFU20_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEU5285112.1"
                     /translation="MSAAHVRRGGPARAKPRKSAPKRSSSRVGHARANRLAGLAFAAF
                     LLAMGVVVVIALDIPSKAATSAGSAIGRAGFTVSGYEIVGLNRMNRALVDAVIVDELR
                     QASDSAGGSKPAQPLVDVADIRNRLLQFGWVKDARVSRRLPDTLVIDIVERQPAALWQ
                     DRQRLALIDREGVVLDRVRVDQMPDLPLLIGPGANARARELDRLMSAVPTLKPQLASA
                     TWIGRRRWDLAFQTGETVALPEGEAAARQALAKFAKVDRSSGLLGRGLVRFDLRVPGK
                     MIVRLPRAPGEAITGEAIATEAPAEG"
     gene            2875..4131
                     /gene="ftsA"
                     /locus_tag="VFU20_01220"
     CDS             2875..4131
                     /gene="ftsA"
                     /locus_tag="VFU20_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU5285113.1"
                     /translation="MSSPAESRLIAALDIGSSKVSALIVTSDDDGRLRVLGTGQRESR
                     GVKRGYITDMEASEFAVREAVELAERMSGVTIDEVWAGYGAGGLYSDVANVEVELGGH
                     QIEQSDIDQLLAGGRNALDRGGQVVLHAHPALYTIDGVEGVKHPIGLHADRLGVDIHV
                     VAADPAPLRNIDYVIRSAHLGVKAIVASPVAAALACLTGEERELGVALVELGAEVTNV
                     SLHAGGMLVGLRSIPLGAKDISDDIACAFGVQRRDAERLKCFYGSAMTSPRDNHEMIE
                     ATQIGSEEGAEPTRITRAQLMMVIRQRVEELTARIDAALKELGFTGPVGRQVVLTGGG
                     AELKNIADYMQGVLGRAVRVGRPRTITGLPDAHSGPAFSTLVGLAFLAGSGSSDIRDI
                     ALGTVTQKKPATGMFGRLIDAMRGGF"
     gene            4306..>4461
                     /locus_tag="VFU20_01225"
     CDS             4306..>4461
                     /locus_tag="VFU20_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU5285114.1"
                     /translation="MSIDFVRPEVDELRPRISVIGVGGAGGNAVANMIAADVQGVDFI
                     VANTDAQA"
BASE COUNT          698 a         1503 c         1601 g          659 t
ORIGIN      
        1 gcaaggcgag ggtgtcgaaa tgagcgggga caaagagacc accgttcgcc ctgagcctgt
       61 cgaagggctg attttgtcta ctccggccgt gaggaaagga cagtccctcg acaagctcgg
      121 gacgaacggg cttgaggtga acgtgcgtgg gcggctgacc aaggatgcgc cgctggcgcc
      181 tttggtgtgg ttcaagagcg gcggcaatgc cgaatggctg ttcgagccgg ccgacgccga
      241 cgacctcgcc gaattcctcc ggggcctcga tcccgacgtg ccggtgatgg cgctggggct
      301 ggggtcgaac ctgatcgtcc gcgacggcgg ggttccggga gtcgtcgtcc ggctcggcaa
      361 ggcgttcgcc aggatcgagc agccggacga cactctgttg cgctgcggcg gcggggcgag
      421 cggcatcctc gtatcctcga ccgctcgcga tgcgggcatc gccgggctcg agtttttgcg
      481 cggcatcccc ggcaccgtcg gcggcttcgt ccggatgaac ggcggcgcct acggccgcga
      541 ggtcaaggac atcctcgtct cggcgacact ggtcctgcgc tcgggcgagg tcgaggaatg
      601 gtcgctcgac aggctcggct acagctaccg acacagcgcg gtgcccgacg ggtcggtggt
      661 gatcgacgcg accttccgcg ggacgcgcgg cgacccggcg aggatcggcg ccgagatgga
      721 ccgaatcgcc gcggcgcgcg aggagaccca gccgatccgc agccgcaccg gcggctcgac
      781 cttcaagaac ccgccggggc acaaggcgtg ggcgctgatc gacgccgccg gttgccgggg
      841 aatgactctg ggcggcgcgc aagtgtcgga aaagcattgc aatttcctgc tcaatctcgg
      901 tagcgcgacg agctccgata tcgaggcgct gggcgaggaa gtccggcggc gggtgcgggc
      961 gaggaccaac atctccctcg aatgggaaat ccagcgggtc ggcagcgagt gaacgtgcgt
     1021 caatgaacaa ggatttgcat gtcgtggtgc tgatgggcgg ctggtcgtcc gagcgcgagg
     1081 tgtcgctgac cagcggccgc ggcgtcgcgc aggcgcttcg gacacgcggc tgggccaacg
     1141 tcaccgagat cgacatggac cgcaacgtcg cccagctgat caccgccgtg cggcccgacg
     1201 tggtgttcaa cgcgctccac gggacccccg gcgaggacgg caccgtccag ggaatgctcg
     1261 acctgatgca gatcccctac acccacagcg ggctcgagac gtcggtgatc gcgatcgaca
     1321 aggagctgac caagatggtg ctggtgccgc acggcatccg catgccgccc ggccggctgg
     1381 tcgagagcga gagcctgtac gcggccgacc cgatgccgcg gccctatgtc ctcaagccgg
     1441 tcaacgaggg ctcgtcggtc ggggtcgcga tcgtcaccga cgacggcaat accggcaacc
     1501 cgatcggccg cgaggtcgaa gggccgtggc atcatttcga caagctgctc gccgagccct
     1561 tcgtcaaggg ccgcgagctg accgtcgctg tgctcggcgg cgaggcgctg gcggtgaccg
     1621 agttgaagcc ggtcgccggc ttctacgatt atgacgcgaa gtacaccgac gggctgacgg
     1681 tccacgtctg cccggccgag attcccgagg acatcgcccg cgcgacgatg gacatggccg
     1741 ccagggcgca ccggctcctg ggctgcaagg gcgcgtcgcg gtccgatttc cgctgggacg
     1801 acgaggcggg cgaggccggc atctttttgc tcgagatcaa cacccagccg ggcatgactc
     1861 cgctgagcct ggtgcccgag caggccaaat attgcggcat cggctacggc gagctggtcg
     1921 agcggctgat cgtcgaagcg ctcggggacg agccatgagc gccgcgcatg tccgccgcgg
     1981 cggcccggcc cgcgccaagc cgcgcaagtc ggcgccgaag cggtcctcga gcagggtcgg
     2041 ccacgcgcgc gccaaccggc tcgccgggct cgccttcgcc gcgttcctgc tggcgatggg
     2101 cgtggtcgtc gtgatcgcgc tcgacatccc gtcgaaggcg gcgacctcgg ccggctcggc
     2161 gatcggccgc gccggcttca ccgtcagcgg ctacgagatc gtcgggctca accggatgaa
     2221 ccgggccctg gtcgacgcgg tgattgtcga cgagcttcgc caggcgtcgg acagcgccgg
     2281 cggctccaag cccgcccagc cgctggtcga cgtcgcggat atccgcaacc ggctgctgca
     2341 gttcggctgg gtcaaggacg cgcgggtgtc gcggcgcctt cccgacacgc tggtgatcga
     2401 catcgtcgag cggcagccgg cggcgctttg gcaggacagg caaaggctcg cactgatcga
     2461 tcgcgaaggg gtcgtgctcg accgggtgcg ggtcgaccag atgcccgacc tgccactgct
     2521 gatcgggccg ggagccaatg cccgggcgcg cgagctcgac cggctgatga gcgcggttcc
     2581 gaccttgaag ccgcagcttg cgtccgcgac gtggatcggc cggcgccgct gggacctcgc
     2641 gttccagacc ggcgagacgg tcgctctgcc cgaaggcgag gccgcggccc ggcaggcgct
     2701 ggccaagttc gccaaggttg accgctcgtc gggcctgctc gggcgcgggc tggtgcggtt
     2761 cgacctgcgc gtcccgggca agatgatcgt ccggctgccg cgcgcccctg gtgaagccat
     2821 caccggcgaa gcgatcgcca ccgaagcccc ggcggaaggg tgatcggcgc aacaatgtcg
     2881 tcgcccgccg aatccaggct gatcgccgcg ctcgacatcg gctcgtccaa ggtcagcgca
     2941 ctcatcgtca cctccgacga cgacggccgg ctgcgcgtgc tcggcaccgg ccagcgcgaa
     3001 agccgcgggg tcaagcgcgg ctacatcacc gacatggaag cgagcgagtt cgcggtccgc
     3061 gaggcggtcg agctcgccga gcgcatgtcg ggggtcacca tcgacgaggt gtgggcgggc
     3121 tacggcgccg gcggcctcta cagcgacgtc gccaatgtcg aggtcgaact cggcggccac
     3181 cagatcgagc agtcggacat cgaccagctg ctggccggcg gccgcaacgc gctcgaccgc
     3241 ggcggccagg tcgtgctcca cgcccacccg gcgctctaca ccatcgacgg ggtcgagggc
     3301 gtcaagcacc cgatcgggct ccacgccgac cggctcgggg tcgacatcca cgtcgtcgcc
     3361 gccgacccgg cgccgctgcg caacatcgat tatgtgatcc gctcggcgca cctgggcgtc
     3421 aaggcgatcg tcgcctcgcc ggtcgccgcc gcgctcgcct gcctgaccgg ggaggagcgc
     3481 gagctgggag tcgccctggt cgagctcgga gccgaggtca ccaacgtctc gctccacgcc
     3541 ggcgggatgc tggtcgggct gcgctcgatc ccgctgggcg ccaaggacat cagcgacgac
     3601 atcgcctgcg cgttcggggt ccagcggcgc gacgccgagc gcctcaaatg cttctacggc
     3661 tcggcgatga cgtccccacg cgacaatcac gagatgatcg aggcgaccca gatcggctcc
     3721 gaggagggcg ccgagccgac ccggatcacc cgcgcgcagc tgatgatggt gatccgccag
     3781 cgggtcgagg agctgaccgc ccggatcgac gcggccttga aggagctggg cttcaccggg
     3841 ccggtcgggc ggcaggtggt cctgaccggc ggcggggccg agctcaagaa catcgccgac
     3901 tacatgcagg gcgtgctcgg ccgggcggtc cgcgtcggcc ggccgcggac catcaccggc
     3961 cttcccgacg ctcacagcgg gccagctttc tcgaccctgg tcgggctggc gttcctggcc
     4021 ggctcgggga gcagcgacat ccgcgacatc gcgctcggca ccgtcaccca gaaaaagccc
     4081 gcgaccggca tgttcgggcg gctgatcgac gcgatgcgcg gcggcttttg acggcgcgaa
     4141 actgcaccag cgcgggacag tgagtcttcg cggcaactat tgtccccaat tttttgttgt
     4201 ccgctgtcgt tttcgctcgc gccgcgaatc ggataatgca atgattcagc cagaaggttc
     4261 agcaccttgc cgcttgttgg ggctgcttga ggggggtttg ggatcatgag catcgatttc
     4321 gtccggcctg aagtcgacga gcttcgcccg cggatcagcg tcatcggagt cggcggggcc
     4381 ggcggcaacg ccgtcgccaa catgatcgcc gccgacgtcc agggggtcga cttcatcgtc
     4441 gccaacaccg acgcccaggc c
//
LOCUS       DASTZO010000047         6801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_656454,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000047 DASTZO010000000
VERSION     DASTZO010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656454"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1471
                     /locus_tag="VFU20_01230"
     CDS             <1..1471
                     /locus_tag="VFU20_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU5285115.1"
                     /translation="EKGGGWHLLVSEMPYGVQKGRLIEQVAALIADKKLPILADVRDE
                     SDEKVRLVLEPRSRTVDPQLLLESLFRLTDLEIRFPLNLNVLDANRTPGVMSLKQALT
                     AWLAFQIEVLLNRSRTRIGKIDERLELLDGFLVAFLNLDRVIAIIRSEDEPKPVLMAE
                     FELSERQAEAILNMRLRQLRKLEEVQIAKEREALARERDELTKLVESPARQRTRLKKD
                     LAAVRTSFGDERRTRIEEAGATREIDWSAMIEKEPITVLLSQRGWLRAMRGHLAREEL
                     EALKWREGDGPFIQFHAQTTDRLALFADNGRVYTLAGDKLPGGRGFGEPVRLLIDLDA
                     EVGIVAFLVARAKSKLLVASSDGRGFVTPGEAVLAETRKGKQLVNLRPGAKVAAVREI
                     PEGADSVATIGENRKMLVFPLSELPELARGQGVTLQRYRDGGLSDALAFRFADGLSWA
                     LGGESGRVRTESDLTPWRAARGAAGRMPPLGFPRSNRFG"
     gene            1672..3315
                     /locus_tag="VFU20_01235"
     CDS             1672..3315
                     /locus_tag="VFU20_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5285116.1"
                     /translation="MLGALPIAAAVITRHDDGSVHVLAHNSRFRDAVDQSTCTALEWD
                     HAECLKSGPIAELLAGYFAGTDSVGELDFRDGEGVAARYFRIKLAPLPRVEGAAPRCL
                     LSVVDRTVEVQAERTLRAEMLRDSLTGLPNRLAFTEAIEKAGDKASRDLEHAVLVVDM
                     LRFSRINESMGSLAGDELLITFARRLILALRGGDVLARTGGNEFGVLVSLRRGVEDAL
                     KAAERIQEVMATPFKLSDLEIRIECAIGVALMDARQDPEELFRNAQFAVKQAKSAGKP
                     QVYEPREASLARRRFSIETELRRALDKDQLKLFYQPLIDLKSGEVAGFEALARWTHEE
                     RGEISPSEFIPVAEESGLILTLGRWAMDKATRTLAGWDREAGEKLPFYVAVNLSAIQV
                     ARDDIAQVVSSALKSSGLSGDRLTLELTESSIVQDPARATRVFEALKALDTTVAMDDF
                     GTGYSSLAYLQRLPIDVLKIDRSFVSGMMVDPDSVAIVRAVLSLAEALGMSTTAEGIE
                     TVELATTLAALGCSSGQGYYFSKPLEPEAAVEYWRSRKR"
     gene            complement(3322..3615)
                     /locus_tag="VFU20_01240"
     CDS             complement(3322..3615)
                     /locus_tag="VFU20_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285117.1"
                     /translation="MRTLAFAALIALAACDGPRENAGENADYAAGVVNSPDTLRQGPA
                     EKLGEAQDIAAKRAEEAREAEARALEAAADEKRALADREAEALEDQADEVRNN"
     gene            complement(3681..4877)
                     /locus_tag="VFU20_01245"
     CDS             complement(3681..4877)
                     /locus_tag="VFU20_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEU5285118.1"
                     /translation="MDLKLDAAKWRRRRGMKRLLKALGADQGLTRYVGGAVRDDLLGL
                     PVSDIDLATRIRPQEVVERLEAVGVKVVPTGIAHGTVTAVSDGLPVEVTSLRRDVSTD
                     GRRATIAYTDDWQEDAARRDFTLNALSADPATGEVFDYFGGLEDLEKRRVRFIGDPKE
                     RIAEDHLRILRFFRFHARFGTGEPDRQALDACAERANDLMALSRERIADELLKLLAMP
                     DPAPTVAVMLDRAILKPVLPEIEPAALPAFKRVIAAERDALIEPGALRRLAALLPPDE
                     PLAEKVAIRMRLSNRARKRLACAAGRELGANPQALGYRLGIDCAVDRLLLAGESARAA
                     ALVGWRPPRLPIGGGKLIARGVPEGPAVARTLRRIEDRWVEAGFPHGAELESIVAEAL
                     ASESAR"
     gene            complement(4868..5461)
                     /locus_tag="VFU20_01250"
     CDS             complement(4868..5461)
                     /locus_tag="VFU20_01250"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEU5285119.1"
                     /translation="MPPPQSKLADRLREALARPPAEPLLTGDDARETGDPAPAAVLVA
                     VTDRPEPGVILTVRHADLRTHAGQIAFPGGRIDAGEDSAAAALREAWEELGLEPDLVE
                     PVAALEPYRTITGFAVSPVLAVIPPDLPLAPHEPEVADWFEAPLAFLLDPANQQRQAM
                     LFEGRMRHYYRIDWNGRNIWGATAAMLVNLSRRLQWT"
     gene            complement(5473..6042)
                     /locus_tag="VFU20_01255"
     CDS             complement(5473..6042)
                     /locus_tag="VFU20_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HEU5285120.1"
                     /translation="MPESRPPIELRGLGLGELQALIDQRRLPPVDRWNPERCGHSGMR
                     IARDGTWYHQGAPIRRPAMVRLFSTVLRREPDGRHMLVTPVEKLEIDVEATAFRAVEM
                     KTEGEHEQRKVALGLDSGDALVVGPDHPLRLAEDEHGPSPRVLVRHGLEAELARPVYY
                     ELAEIALAEGHDPPGVWSDGAFFALDRPQ"
     gene            6130..>6801
                     /locus_tag="VFU20_01260"
     CDS             6130..>6801
                     /locus_tag="VFU20_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5285121.1"
                     /translation="MDARTAIVTGAGKRVGAEIARALVGDGWSVVAHVRREEDSVPDG
                     AVRVAADLADAGCAGTIFAAARGLPPVRLLVNNAARFAWDGFGEFSSAEFDAHMAVNV
                     RAPLLLTEAFAAAHDGKADALVVNLLDSKLAAPNPDFLSYTLSKQALAGLTELSARAL
                     AARGIRVNGVAPSLMLRSAGQSEENFAAMQRANPLRRGVEPGDVVAALRYLVAAPAVT
                     GQVLTI"
BASE COUNT          986 a         2351 c         2468 g          996 t
ORIGIN      
        1 cgagaaaggc ggcggctggc accttttggt cagcgaaatg ccctatggcg tccagaaggg
       61 ccggttgatc gagcaggtcg cggcgctgat cgccgacaag aagctgccga tcctcgccga
      121 cgtccgcgac gagagcgacg agaaagtgcg cctggtgctc gagccgcggt cgcggacggt
      181 ggacccgcaa ttgctgctcg agagcctctt ccggctgacc gacctcgaga tccgcttccc
      241 gctcaatcta aacgtgctcg acgccaaccg gactccgggc gtgatgagcc tgaagcaggc
      301 gctgaccgcc tggctcgcgt tccagatcga ggtgcttctc aaccgctcgc gcacccgtat
      361 cggcaagatc gacgagcggc tggagctgct cgacggcttc ctggtcgcct tcctcaacct
      421 cgaccgggtg atcgccatca tccgcagcga ggacgagccg aagccggtgc tgatggccga
      481 gttcgagctg agcgagcgcc aggccgaggc gatcctcaac atgcgcctgc ggcaattgcg
      541 caagctggag gaggtgcaga tcgccaagga gcgcgaggcg ctggccaggg agcgcgacga
      601 gctcacgaag ctggtcgaga gcccggcgcg gcagcgcacc cggctgaaga aggacctcgc
      661 cgcggtccgg acaagcttcg gcgacgagcg gcggacccgg atcgaggagg cgggcgcgac
      721 ccgcgagatc gactggtcgg cgatgatcga gaaggagccg atcaccgtcc tgctgtcgca
      781 gcgcggctgg cttcgggcaa tgcgcgggca cctggcgcgc gaggagctcg aggcgctcaa
      841 gtggcgcgag ggcgacgggc cgttcatcca gttccacgcc cagaccaccg accggctggc
      901 tttgttcgcg gacaacgggc gggtctacac gctggccggc gacaagctcc ccggcgggcg
      961 cggcttcggc gagccggtgc ggctgctgat cgacctcgac gccgaggtcg ggatcgtcgc
     1021 cttcctggtc gcgagggcca agtcgaaatt gctggtcgcc tcgtccgacg ggcgcgggtt
     1081 cgtcactccg ggcgaggcgg tcctggccga gacccgcaag ggcaagcaat tggtcaatct
     1141 gcgccccggg gcgaaggtcg cggcggtgcg cgagatcccg gaaggcgcgg attcggtggc
     1201 gacgatcggc gagaaccgca agatgctggt gttccctttg tccgagcttc ccgagctggc
     1261 ccgcgggcag ggcgtgaccc tgcagcgcta tcgcgacggc ggcctgtcgg acgcactcgc
     1321 cttccgcttc gccgacggcc tcagctgggc gctcggcggc gaaagcgggc gggtgcgcac
     1381 cgaaagcgac ctgaccccat ggcgggccgc gcgcggagcg gccgggcgaa tgccgccgct
     1441 gggtttcccg cggagcaacc ggttcggctg aggccaggcg gactgaccgg cccgccatta
     1501 accatcattc atcaagccgt ttcgacatgg aattataccc ggaattaacc tcgcgcccgt
     1561 agggcaatcg ccatgcaggt tgcagccaaa cgtccgccgg gacccgtggg gcaccggcgg
     1621 agggcgcctt ccgatgccca cccgcacgtt gcgacggcgg acgatgccgc attgctcggg
     1681 gcgctgccga tcgccgccgc ggtcatcacc cggcacgacg acggcagcgt ccacgtcctc
     1741 gcgcacaatt cgcgcttccg cgacgccgtc gaccagtcga cctgcacggc gctcgaatgg
     1801 gaccatgccg agtgcctgaa gtcgggcccg atcgccgagc tgctggccgg atatttcgcc
     1861 ggcacggaca gcgtcggcga gctcgatttc cgcgacggcg aaggggtcgc cgcgcgctat
     1921 ttccggatca agctggcgcc gctgccgcgc gtcgagggcg ccgccccgcg ctgcctgctg
     1981 agcgtcgtcg accgcaccgt cgaggtgcag gccgagcgca cgcttcgcgc cgagatgctg
     2041 cgcgacagcc tgaccggcct gcccaaccgg ctggcgttca ccgaggcgat cgagaaagcc
     2101 ggcgacaagg cctcccgcga cctcgagcat gcggtgctgg tggtcgacat gctgcgcttc
     2161 agccggatca acgaatcgat gggcagcctc gccggcgacg agcttctgat caccttcgcc
     2221 aggcggttga tcctggcctt gaggggcggc gacgtgctcg cccgcaccgg cggcaacgag
     2281 ttcggggtgc tggtgtcgct gcgccgcggg gtcgaggacg cgctcaaggc ggcggagcgc
     2341 atccaggaag tgatggcgac cccgttcaag ctgtccgacc tcgagatccg catcgaatgc
     2401 gcgatcgggg tcgcgctgat ggacgcccgg caggaccccg aggagctgtt ccgcaacgcc
     2461 cagttcgcgg tcaagcaggc caaatcggcc ggcaagccgc aggtctacga gcccagggaa
     2521 gcgagcctcg cccggcgccg cttctcgatc gagaccgagc tccgccgggc gctcgacaag
     2581 gaccagttga agctgttcta ccagccgctg atcgacctga agtcgggcga ggtcgcgggc
     2641 ttcgaggctc tggcgcgctg gacccacgag gagcgcggcg agatcagccc gagcgagttc
     2701 atccccgtcg ccgaggaaag cgggctgatc ctgacgctcg gccgatgggc gatggacaag
     2761 gcgacgcgca cgctcgccgg gtgggaccgc gaggcgggcg agaagctgcc cttctacgtc
     2821 gccgtcaacc tgtcggcgat ccaggtcgcc cgcgacgaca tcgcccaggt ggtgtcgagc
     2881 gctttgaagt cgagcgggct gagcggcgac cggctgaccc tcgagctcac ggaaagctcg
     2941 atcgttcagg acccggcgcg ggcgacccgc gtattcgagg cgctgaaggc gctcgacacg
     3001 acggtggcga tggacgattt cggaaccggc tactcgagcc tcgcctacct ccagcggctg
     3061 ccgatcgacg tcctcaagat cgaccgcagc ttcgtctcgg ggatgatggt cgaccccgac
     3121 agcgtggcga tcgtccgggc agtgctcagc cttgccgagg cgctcgggat gtcgacgacc
     3181 gccgaaggca tcgagacggt cgagctggcg accaccctgg cggcgctcgg ctgctcgagc
     3241 gggcagggct attatttctc caagccgctc gagccggaag ccgcggtcga atattggcgc
     3301 tcgcgcaagc ggtagcgccg gctaattgtt gcggacctcg tcggcctggt cctcgagcgc
     3361 ttcggcttcc ctgtcggcaa gcgcgcgctt ttcgtcggcc gccgcctcga gcgcccgggc
     3421 ctcggcttcc ctggcttcct cggcgcgctt ggcggcgatg tcctgggcct cgccgagctt
     3481 ttcggccggg ccctggcgca gcgtgtccgg gctgttgacg acgccggccg catagtcggc
     3541 gttctcgccg gcgttctcgc gcgggccgtc gcaggcggcc agcgcgatca gggcggcaaa
     3601 ggcgagcgtt cgcattgtcc ggtcctccag tccaccgaac aacggtttgc gaccgcgcat
     3661 gttgcgcggg ccgtcgccct ttagcgggcg ctttcggacg cgagcgcctc ggcgacgatg
     3721 ctctccaact cggcgccgtg cgggaagccg gcctcgaccc agcggtcctc gatccggcgc
     3781 agagtccgcg ccaccgccgg gccctccgga actccgcgcg cgatcaattt gccgccgccg
     3841 atcggcagcc gcgggggccg ccagccgacc agcgccgcgg ccctggccga ttcgccggcg
     3901 agcagcagcc ggtcgaccgc gcagtcgatc ccgagccggt agccgagcgc ctgcgggttg
     3961 gcgccgagct cgcggccggc cgcgcaggcg agccgcttgc gcgcccggtt ggacagcctc
     4021 attcggatag cgaccttctc tgccagcggc tcgtcgggcg gaagcagcgc tgcgagccgg
     4081 cggagcgcgc cgggctcgat cagcgcgtcg cgctcggcgg cgatcacgcg cttgaaagcg
     4141 ggaagggcgg ccggctcgat ctcgggcagc accggcttca ggatcgcccg gtcgagcatc
     4201 accgcgacgg tcggcgccgg gtcgggcatc gccagcagct tcagcaattc gtcggcgatg
     4261 cgctcgcgcg acagggccat caggtcgttg gcgcgctcgg cgcaggcgtc gagcgcctgg
     4321 cgatcgggct cgccggtgcc gaagcgggcg tggaagcgga agaagcgcag gatccgcaaa
     4381 tggtcctcgg cgatgcgctc tttggggtcg ccgatgaagc gcacccggcg cttctcgagg
     4441 tcctcgagcc cgccgaaata gtcgaacacc tcgccggtcg cggggtcggc gctgagcgca
     4501 ttgagggtga aatcgcgccg cgccgcgtcc tcctgccagt cgtcggtgta ggcgatcgtc
     4561 gcccgccgcc cgtcggtcga gacgtcgcgg cgcaggctgg tgacctcgac cggaaggccg
     4621 tcgctgaccg cggtgacggt gccgtgcgcg atccccgtcg ggaccacctt gaccccgacc
     4681 gcctcgagcc gctcgaccac ttcctgcggg cggatccggg tcgcgaggtc gatgtcgctg
     4741 accggcagcc ctagcaaatc gtcgcgcacc gcgccgccga cgtagcgggt caggccctgg
     4801 tcggcgccga gcgcctttaa aagccgcttc atgccgcgcc ggcggcgcca tttcgcggcg
     4861 tcgagcttca agtccattgc agcctgcgcg aaagattgac gagcatcgcc gcggtcgccc
     4921 cccagatgtt ccggccgttc cagtcgatgc ggtaataatg gcgcatccgg ccctcgaaca
     4981 gcatcgcctg gcgctgctgg ttggcgggat cgaggagaaa cgcaagcggc gcttcgaacc
     5041 agtcggcgac ctcgggctcg tgcggtgcca gcggcaggtc cggggggatg acggcgagca
     5101 ccgggctcac ggcgaagccg gtgatggtcc ggtagggctc cagcgcggcg accggctcga
     5161 ccaggtccgg ctcgagcccc agctcctccc aggcctcgcg caaggccgcg gcggccgaat
     5221 cctcgccggc gtcgattcgg ccgccgggaa aggcgatctg gccggcgtgg gtgcgcaggt
     5281 cggcatggcg gacggtgagg atcactccgg gctcgggccg gtcggtgacc gcgaccagca
     5341 ccgcggccgg cgccggatcg ccggtttcgc gagcatcgtc gccggtcagc agcggctcgg
     5401 ccggcggccg ggcgagggcc tcgcggagcc ggtcggccag cttgctctgc ggcggcggca
     5461 tggcggcggt cctcattgcg gccggtcgag cgcgaagaag gcgccgtcgc tccacacgcc
     5521 cggcgggtcg tggccctcgg cgagcgcgat ttcggcaagc tcgtaataga ccgggcgggc
     5581 gagctcggcc tcgagcccgt ggcggacgag gactcgcggg ctcggcccat gctcgtcctc
     5641 cgccagccgc agcgggtggt cggggccgac gaccagcgcg tcgccgctgt cgaggccgag
     5701 cgcgaccttg cgctgctcat gttcgccctc ggttttcatc tcgacggcgc ggaaagccgt
     5761 cgcctcgacg tcgatctcga gcttttcgac cggcgtgacc agcatgtggc ggccgtcggg
     5821 ctcgcggcgc aggaccgtcg agaacagacg gaccattgcc ggccggcgga tcggggcgcc
     5881 ctgatggtac caggtgccgt cgcgggcgat gcgcatgccc gaatggccgc agcgctccgg
     5941 gttccaccgg tcgaccggcg gcagccggcg ctggtcgatc agcgcctgca gctcgccaag
     6001 gccgaggcct cgaagctcga tcggggggcg gctctcgggc atggggcccc tgtgtcattg
     6061 cgagcagagc gaagcaatcc acgcacaaac cggattgctt cgccgcgatg ctgctcgcaa
     6121 tgacggccaa tggatgctcg aaccgcgatc gtcaccgggg ccggcaagcg cgtcggggcc
     6181 gagatcgccc gggcgctggt cggcgacggg tggagcgtcg tcgcccacgt ccgccgcgag
     6241 gaggattcgg tgcccgacgg agcggtcagg gtggccgccg acctcgccga cgccggctgc
     6301 gccgggacca tcttcgcggc cgcgcggggg ctgccgccgg tgcggctcct ggtcaacaat
     6361 gccgcccgct tcgcgtggga cgggttcggc gagttcagct ccgccgagtt cgacgcccac
     6421 atggcggtca acgtgcgggc gccgctgctg ctgaccgagg cgttcgcggc cgcgcacgac
     6481 ggcaaggccg acgcgctggt ggtcaatctc ctcgattcga agctggccgc gcccaacccc
     6541 gatttcctca gctacacatt gtccaagcag gcgctcgccg ggctcaccga gctgtcggct
     6601 cgggcgctcg ccgccagggg catccgggtc aatggcgtgg cgccgtcgct gatgctgcgc
     6661 tcggccgggc agagcgagga gaatttcgcc gcgatgcagc gcgccaaccc gctgcgccgc
     6721 ggagtcgagc cgggcgacgt cgtcgccgcg ctgcgctatc tggttgcggc ccccgcggtg
     6781 accggtcagg tgctgaccat c
//
LOCUS       DASTZO010000048         6638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_395077,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000048 DASTZO010000000
VERSION     DASTZO010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6638
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395077"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="VFU20_01265"
     CDS             <1..369
                     /locus_tag="VFU20_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5285122.1"
                     /translation="ELPEAEGVLGALAGDAAGHPAIARARAALELASVPPVDTAALEA
                     RVAASPDDHEARFELAAARMGAGDRDGAADELLEIIGRDRDWNEGAARKRFLQLLEAQ
                     GLEDPWARSQRRRLSALLFT"
     gene            383..1021
                     /locus_tag="VFU20_01270"
     CDS             383..1021
                     /locus_tag="VFU20_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HEU5285123.1"
                     /translation="MTKALILRVPIFPLAGAILFPRAQLPLHIFEERYREMVRDSLDG
                     AGTIGMIQPLSLDPVNPPLYGVGCVGEIVGVEELEDGRYNIVLSGSGRFRLVAETELG
                     TPYRNADVDVAAFDDREPPPLPLALRADVEQEARRLGDVLGLAVDWAAVGRLDDEMLV
                     NAIAQVAPFDVGAKQALLEAESLDSRADLLVQLMQFQRMATSAGADIEPTLQ"
     gene            complement(1022..2251)
                     /locus_tag="VFU20_01275"
     CDS             complement(1022..2251)
                     /locus_tag="VFU20_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiH/UbiF/VisC/COQ6 family ubiquinone
                     biosynthesis hydroxylase"
                     /protein_id="HEU5285124.1"
                     /translation="MDRADVIIFGGGMIGLALAAALDSSGLSAIVVDPADPDERKDSA
                     FDGRTSAVSSSSMRMLRTIGVADHLPGEGGPILKIQVADGLEPGGLAFEPGADDEPLG
                     WMHENRHLRGALRARAEAGANSWLLWKSRVTAVERGAHGVIVDLADGRKLFAPLLAAA
                     DGRNSPTREAAGIRVARWRYDHSAIVSTLRHERSHEQIAYEIFYPTGPFALLPMADDA
                     NAPGGGRRSAIVWSVPAADTAGWLSLSDSDFAAEARAAMGGFLGEVAMAAPRSTYPLG
                     FHHAARITAHRLALLGDAAHAIHPIAGQGLNLGFRDAAALAEVLVEGARLGLDLGDRQ
                     LTDRYQRWRSLDALMVAMATDGLTRIYGVPGRAASLVRRFGMGVIDRVRPVKDQLMAE
                     ARGTSGDLPLLLRGLPI"
     gene            2353..4686
                     /locus_tag="VFU20_01280"
     CDS             2353..4686
                     /locus_tag="VFU20_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HEU5285125.1"
                     /translation="MATAAARARKRDLGPDWRDALAASLRRFALRSWGALLVAVSVGG
                     ALALATHRSTDPSFTTAAGGPPGNWLGSVGAYASDALLLLFGLGSVLLLPVIALAGLR
                     MLRLEPSGRIGRGLLIAAIGAVLVGIALSLLSGSAVSGLPSGWGGALALAAANGVDFG
                     VAQIGNPSVEGPVRLTLLLLFAVSGLALGYWSMGLQPDEKGWLAGLLRRDARTRAAAP
                     RRTEDPRDERGAAAAPPRSRPAVAVTEPAPAVASAGRGTAGRKSSAARQPSLAFGGVY
                     TLPPLDLLARPPESGRTQIDRAGLERNARLLESVLEDFHVRGDIVEVRPGPVVTMYEL
                     EPASGIKASRVIQLADDIARNMSALSARVATIPGRSVIGIELPNPKREPVTLSELIGS
                     QAFEDQNMSLPLILGKNIAGDPVIADLAPMPHLLVAGTTGSGKSVGLNCMILSLLYRM
                     TPDECRLILIDPKMLELSTYEDIPHLLAPVVTEAGKAIRALKWTVEQMEERYRMMANL
                     GVRALPSFNAKVRDAKAKGAQLGRRVQTGYNADTGQPIYEVEELDYDVLPQIVVVIDE
                     LADLMMTAGKEVEFLVQRLAQKARAAGIHLIMATQRPSVDVITGVIKANLPTRISFQV
                     TSKIDSRTILGEQGAEQLLGKGDMLYMPGGKQIVRVHGPLVRDEEVRAVAEHWRKQGV
                     PEYIQAVTEEPEDGGYLFDGQPTGEDDAETQLYKKAVQIVAESQKASTSYLQRQLRVG
                     YNSAARLIERMEKDGKVGQPDHVGRREVLIDPEGHPL"
     gene            4743..4928
                     /locus_tag="VFU20_01285"
     CDS             4743..4928
                     /locus_tag="VFU20_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285126.1"
                     /translation="MLRSLLPAFAVLALVAASGACSPAEPPLLIDGGQAPASDVGSEA
                     PSSEPPAAGSDKQAVSG"
     gene            5041..5676
                     /locus_tag="VFU20_01290"
     CDS             5041..5676
                     /locus_tag="VFU20_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEU5285127.1"
                     /translation="MSFPTLFARALVSIAAPVALVASAVPAAAAAESPDFARVQAHLK
                     AVQSMTANFVQTDGKGRSARGTLQLKRPGRIRFEYSGGDLLLVANGSRLTFVDYEVGQ
                     KSSWDLNKTPLGMLLSVNPILKGNARLVPSGDPRTVVIRAKDRNRPEYGTLILAFLRS
                     PSAPGGLMLYGWTAVDAQNKRTTVKLSDQRYNVAVAEGAFTFAEPKKRKRG"
     gene            5897..>6638
                     /locus_tag="VFU20_01295"
     CDS             5897..>6638
                     /locus_tag="VFU20_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU5285128.1"
                     /translation="MTRIKIASWNINSVRARAGLVERLVAEEQPDVLCLQETKAVNEL
                     FPAELFRKHGYVHQQLHGQKMHHGVAIVSRIELHEPGSHDWQDNGEARHIGVRLPGGI
                     RLENVYVPAGGDIPDRSLNPKFGQKLDFIERMTRWSEKLREPTLVVGDFNVAPLECDV
                     WSHKALLKVVSHTPVEVEALTRLQQAHDWVDIGRSFVPAPTRNYTWWSYRSPDWTKND
                     RGRRLDHMWVSPEAKGRVVSHKVLEPCRS"
BASE COUNT         1001 a         2323 c         2386 g          928 t
ORIGIN      
        1 gaattgcccg aggccgaggg cgttctcggc gcgcttgccg gcgatgccgc cggccacccg
       61 gcgatcgccc gcgcccgggc ggcgctcgag ctggcgtcgg tgccgccggt cgacaccgcg
      121 gcgctcgagg cgcgcgtcgc cgccagcccc gacgaccatg aggcgcgctt cgagctcgcc
      181 gcggcgcgga tgggcgccgg cgaccgcgac ggggccgccg acgagctgct cgagatcatc
      241 ggccgcgacc gcgactggaa cgagggcgcg gcgcgcaagc gcttcctgca attgctcgag
      301 gcgcaggggc tcgaggaccc gtgggcccgc agccagcggc ggcggctgtc ggcgctcctg
      361 ttcacgtgag gccgccagca gcgtgaccaa ggcgctgatc ctgcgggtgc cgatcttccc
      421 gcttgccggc gcgatcctgt tcccccgcgc ccagctgccg ctgcacatct tcgaggagcg
      481 ctaccgcgag atggtccgcg attcgctcga cggcgccggg accatcggca tgatccagcc
      541 gctcagcctc gatccggtca acccgccgct ctacggggtc ggctgcgtcg gcgagatcgt
      601 cggagtcgag gagctggaag acggccgcta caacatcgtg ctgagcgggt caggccggtt
      661 ccggctggtc gccgagaccg agctcgggac tccctaccgc aacgccgacg tcgacgtcgc
      721 cgcgttcgac gaccgcgagc cgccgccgct gccgctggcg ctccgcgccg acgtcgagca
      781 ggaggcgcgc cgcttgggcg acgtgctcgg gctggcggtc gactgggcgg cggtcgggcg
      841 gctcgacgac gagatgctgg tcaacgcgat cgcccaggtg gcgccgttcg acgtcggcgc
      901 caagcaggcc ttgctcgagg cggagagcct cgacagccgc gccgacctcc tggtccagct
      961 gatgcagttc cagcgcatgg cgaccagcgc cggggcggac atcgagccga cgctgcaata
     1021 atcagatcgg caagcctctg agcagcagcg gcaggtcgcc gctggtgccg cgggcttcgg
     1081 ccatcagctg gtccttcacc ggccgcaccc ggtcgatcac gcccatgccg aagcgtcgga
     1141 ccagcgacgc ggcgcgcccg ggcacgccgt agatgcgcgt caggccgtcg gtcgccatgg
     1201 ccaccatcag cgcatcgagc gagcgccagc gctggtagcg gtcggtgagc tgccggtcgc
     1261 cgaggtcgag cccgagccgc gcgccctcga ccagcacctc ggcgagcgcg gcggcgtcgc
     1321 ggaagccgag gttgaggccc tggccggcga tcgggtggat cgcgtgggcg gcatcgccga
     1381 gcagcgccag gcgatgcgcg gtgatccggg ccgcatgatg gaagcccagc ggataggtcg
     1441 agcgcggcgc ggccatcgcg acctcgccga ggaagccgcc catcgcggcg cgcgcctcgg
     1501 cggcgaagtc cgagtccgac agcgacagcc agccggcggt gtccgcggcg gggaccgacc
     1561 agacgatcgc cgagcggcgg ccgccacccg gggcattggc atcgtcggcc atcggcagca
     1621 gggcgaaggg tccggtcggg tagaagatct cgtacgcgat ttgctcatgg ctgcgctcgt
     1681 ggcgcagggt cgagacgatc gccgaatggt cgtaacgcca gcgcgcgacc cggatgcccg
     1741 ccgcctcgcg ggtcggcgag ttgcgcccgt cggcggcggc gagcagcggc gcaaagagct
     1801 tgcggccgtc ggccaggtcg acaatcactc cgtgcgcgcc gcgctcgacc gcggtgaccc
     1861 gcgatttcca cagcagccag ctgttggccc cggcttcggc gcgggcgcgc agggccccgc
     1921 gcaggtggcg gttctcgtgc atccagccca ggggctcgtc gtcggcgccc ggctcgaaag
     1981 cgagcccgcc cggctcgagc ccgtcggcga cctggatctt gaggatcggc ccgccctcgc
     2041 cgggcaggtg atcggcgacg ccgatcgtcc ggagcatgcg catcgagctc gacgagaccg
     2101 cgctggtgcg gccgtcgaaa gccgaatcct tgcgctcgtc gggatcggcg gggtcgacga
     2161 cgatcgccga cagcccgctc gagtcgaggg cggcggcgag cgcgaggccg atcatgcccc
     2221 cgccgaaaat gatcacgtcg gcccggtcca tcgggtgtgt ccctaacgcc acgacgcccg
     2281 gaaggccaac gcttgaccgt ggagtccgcg gcaaggcatt ctacgcgccg gacgatttgg
     2341 ggtgggtgaa ccatggcgac cgctgcggcg cgggcaagga aacgggacct ggggcccgac
     2401 tggcgcgacg ctctggcggc gtcgctccgg cgcttcgcgc tgcgcagctg gggcgcgctg
     2461 ctggtcgcgg tgagcgtcgg cggggcgctg gcgctcgcca cccaccgctc gaccgacccg
     2521 agcttcacca ccgcggccgg aggcccgccg ggcaactggc tgggaagcgt cggcgcttac
     2581 gccagcgacg ccctgctgct gctgttcggg ctcgggtcgg tgctgctgct gccggtgatc
     2641 gcgctcgccg ggctgcgcat gcttcggctc gagccgtcgg ggcggatcgg ccgcgggctg
     2701 ctgatcgcgg cgatcggggc ggtgctggtc gggattgcgc tcagcctgtt gagcggctcg
     2761 gcggtgtccg ggctgccgag cggctggggc ggggcgctgg cgctcgccgc cgccaacggg
     2821 gtcgacttcg gagtcgccca aatcggcaac ccgtcggtcg aagggccggt ccgcctgacc
     2881 ctgctgctgc tgttcgcggt cagcgggctg gcgctcggct attggtcgat gggcctccag
     2941 cccgatgaga aaggctggct ggccgggctg ctgcgccgcg atgcgcggac cagggccgcg
     3001 gctccgcgcc ggaccgagga cccgcgcgac gagcgcggcg ccgccgccgc tccgccgcgg
     3061 tcgcgcccgg cggtcgcggt caccgagccg gcgccggcgg tggcctcggc gggacggggg
     3121 acggccggcc gcaagtcgag cgcggcgcgc cagccgagcc tcgcgttcgg cggggtctat
     3181 acgctaccgc cgctcgacct tctcgcccgg ccgccggaaa gcgggcggac gcagatcgac
     3241 cgcgccgggc tcgagcgcaa tgcgcggctc ctggaatcgg tgctcgagga cttccacgtc
     3301 cgcggcgaca tcgtcgaggt ccgcccgggc ccggtggtga cgatgtacga gctcgaaccg
     3361 gcaagcggca tcaaggcgag ccgggtgatc cagctcgccg acgacatcgc ccgcaacatg
     3421 tcggcgctat cggcgcgggt cgcgaccatc ccgggccgga gcgtgatcgg gatcgagctg
     3481 cccaacccca agcgcgagcc ggtgaccctc tccgagctga tcggcagcca ggcgttcgag
     3541 gaccagaaca tgtcgctgcc gctgatcctc ggcaagaaca tcgccggcga cccggtgatc
     3601 gccgacctgg cgccgatgcc gcatttgctg gtggcgggga cgaccggctc gggcaagtcg
     3661 gtcggcctca actgcatgat cctgtccttg ctctaccgga tgacccccga cgagtgccgg
     3721 ctgatcttga tcgaccccaa gatgctcgag ctgtcgacct acgaggacat cccgcatttg
     3781 ctcgcgccgg tggtgaccga ggcggggaaa gcgatccgcg cactcaaatg gaccgtcgag
     3841 cagatggagg agcgctaccg gatgatggcc aatttgggcg tccgggcgct gccgagcttc
     3901 aacgccaagg tgcgcgacgc caaggctaag ggcgcgcagc tcgggcggcg ggtgcagacc
     3961 ggctacaacg ccgacaccgg ccagccgatc tacgaggtcg aggagctcga ctacgacgtg
     4021 ctgccgcaga tcgtggtggt gatcgacgag ctggccgacc tgatgatgac cgccggcaag
     4081 gaggtcgagt tcctcgtcca gcgtctcgcg caaaaggcgc gggcggcggg catccacctg
     4141 atcatggcca cccagcggcc gtcggtcgac gtcatcaccg gggtcatcaa ggccaatttg
     4201 ccgacccgca tcagcttcca ggtgacgagc aagatcgaca gccgcaccat cctcggcgag
     4261 cagggcgccg agcagctgct cggcaagggc gacatgctct acatgccggg cggcaagcag
     4321 atcgtccggg tccacggccc gctggtccgc gacgaggagg tgcgcgccgt cgccgagcat
     4381 tggcgcaagc agggcgtgcc cgaatatatc caggcggtga ccgaggaacc cgaggacggc
     4441 ggctatctgt tcgacggcca gccgaccggc gaggacgacg ccgagaccca gctctacaag
     4501 aaagcggtgc agatcgtcgc cgagagccag aaggcgtcga cctcctacct ccagcgccag
     4561 ctccgcgtcg gctacaacag cgcggcccgg ctgatcgagc ggatggaaaa ggacggcaag
     4621 gtcggccagc ccgaccatgt cggccggcgc gaggtgctga tcgaccccga agggcacccg
     4681 ctctagcttt tcttcgcctt cgattcatcg ctgattcagc actggtcgcc tatggtgcgc
     4741 cgatgctgcg cagcctgctc cccgcctttg ccgtcctggc cctcgtcgcg gcttccggcg
     4801 cctgcagccc ggccgaaccg ccgctgctga tcgacggcgg ccaggcgccg gcgagcgacg
     4861 tcggctccga ggccccctcg tccgagccgc cggccgccgg cagcgacaag caggcggttt
     4921 cgggctgagc gagcggcgcg gccacgcctg aaccgcgggg aaccgacggg ttcagggggc
     4981 attcaatccc cgagcgtgat agcccacgcc aatcttcgca aaatctcccg ggagcatgag
     5041 atgagttttc caaccctgtt cgcgcgcgcc ctggtgtcga tcgccgcgcc ggtcgcgctg
     5101 gtcgcctccg cggtgccggc ggcggccgcg gccgaatcgc ccgacttcgc ccgggtccag
     5161 gcgcatctga aggcggtcca gtcgatgacc gcgaacttcg tccagaccga cggcaagggc
     5221 cgctcggcgc gcggcaccct tcaattgaag cggccgggcc ggatccgctt cgaatatagc
     5281 ggcggcgacc tgcttctggt cgccaacggc agccggctga ccttcgtcga ctacgaggtc
     5341 gggcagaaat cgagctggga cctcaacaag accccgcttg ggatgctgct gtcggtcaac
     5401 ccgatcctca agggcaatgc gcggctggtt ccgagcggcg acccgcgcac cgtcgtcatc
     5461 cgcgccaagg accgcaaccg gcccgagtac gggaccctga tcctcgcctt cctgcgctcg
     5521 ccgtcggcgc cgggcgggct gatgctctac ggctggaccg cggtcgatgc gcagaacaag
     5581 cggaccaccg tcaagctgtc ggaccagcgc tacaatgtcg cggtcgcgga aggcgcgttc
     5641 accttcgccg agccgaagaa aaggaagcgc ggataaaagg gtagccgttc gctacttgtt
     5701 catcgcgccg tcagccgggc ggtcctaaat aggtcgcggt cctgcatctt gcttggggtt
     5761 tccccctgtt acccgggcac cgcaggatcc cttgcgtgac gaacgctcgg cggccctcgc
     5821 tccacgcccc cggagcgggg gccttttgcg ttcggccggc tcgcaaagac cgcggaggct
     5881 ggctacatcg gccggcatga cccgtatcaa gatcgcctcg tggaacatca actccgtgcg
     5941 cgcccgcgcc gggctggtcg agcgcctggt cgccgaggag cagcccgacg tcctgtgcct
     6001 gcaggagacc aaggcggtca acgagctgtt cccggccgag ctgttccgca agcacggcta
     6061 cgtccaccag cagctgcacg gccagaaaat gcaccatgga gtcgccatcg tcagccggat
     6121 cgagctccac gagcccggca gccacgactg gcaggacaat ggcgaggcgc gccacatcgg
     6181 ggtccggctg ccgggcggga tccggctcga gaacgtctat gtgccggccg gcggcgacat
     6241 tcccgaccgc agcctcaacc ccaagttcgg ccagaagctc gacttcatcg agcggatgac
     6301 ccgctggtcg gaaaagttgc gcgagccgac tttggtggtc ggcgacttca acgtcgcgcc
     6361 gctggaatgc gacgtctgga gccacaaggc gctgctcaag gtcgtcagcc acacgccggt
     6421 cgaggtcgag gcactgaccc ggctgcagca ggcgcacgac tgggtcgaca tcgggcgcag
     6481 cttcgtgccc gcgccgaccc gcaactacac ctggtggagc tatcgctcgc ccgattggac
     6541 gaagaacgat cggggccggc ggctcgacca tatgtgggtg tcgcccgaag cgaagggtcg
     6601 agtggtttcg cacaaggtgc tggaaccgtg ccggagct
//
LOCUS       DASTZO010000049         7011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_787048,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000049 DASTZO010000000
VERSION     DASTZO010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7011
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787048"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VFU20_01300"
     CDS             <1..411
                     /locus_tag="VFU20_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285129.1"
                     /translation="ALASMPASAVDAGTKGPAEKPARSAADTTGGQTEPVPSSPRIGI
                     AQFFTDTDPRTGRRSMRAYEAVKTLEPDQPIGSQGFDLVLSGRLKALPGKSVIACVAR
                     SADSPPDCIVSADFDRVWIAAPADRAIIAEWGGG"
     gene            complement(440..1714)
                     /locus_tag="VFU20_01305"
     CDS             complement(440..1714)
                     /locus_tag="VFU20_01305"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5285130.1"
                     /translation="MNPGSGNPSGAPLPSLTRRSLLAAAGGAALSPAIARAAQLGSKS
                     HVAVVGAGVFGAWTAHHLLAAGHRVTLIDAHGPAHSRASSGGESRMTRAAYGKDAVYT
                     RMAFDSLPQWKALSALAGLPIFVPHGVLFFSSKPDSYFADSVAVHRRLGVPLQELTGP
                     EMSRRFPMIDFTGVSAGLFEPGFGALMARRSVQTLVERFVRGGGQYRHEAAHVVQQQS
                     GRLASLGLRGGSALQADYYVFALGPWLPKLFPDVIGGRILPTRQEVFFFAPPAGDRRF
                     LPAAMPGWADFNEGDIFYGFPELEGRGVKFAHDAHGAAVDPDTQDRRPTAAALAEIVA
                     FRDRRFPLLRGAPLTEARVCQYENSSNGDFLIDFHPRLPNVLLVGDGSGHGFKHGPEV
                     GRYAAARLVGSIEPEPRFSLATKAETHKREVH"
     gene            complement(1727..4312)
                     /locus_tag="VFU20_01310"
     CDS             complement(1727..4312)
                     /locus_tag="VFU20_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5285131.1"
                     /translation="MRTSALLLIGTAAIAISTPVFAQTTEEDVAQAANQPEEASAGQD
                     IIVTATKRASRVQDVPFSINAQTQEDIQRANASTIEEISRNVAGLTVQNLGPGQSQVS
                     VRGVSAGQIVRDQPGVKEQVGVYLDESVISLSLFTPDFDLFDLNRVETLRGPQGTLFG
                     SGSVGGTLRYITNQPELGTTEGMVEGNINTVDEGDIGGHVKGSVNFPLGSAAATRLVG
                     YGTRYGGFVDAIGPAAGKNINDGTRIGGRIALLFEPAPDVKITPRLVYQQIRTDGFNR
                     EEHYNFYTSLTTEPEVGKRKQYLKLREKFKDDTLLADLTASVGFGGVELTSITSYIDR
                     DILVSRDASALTGSVGIVPLVGCCGVDPDIANLASNLRDTTDLKQWTQEVRLASAGSG
                     PLQWVVGVFYTDIDREYAQRLPTPGYDAFIDAALGAGVSAAVSNGFPLNSPYNADLPY
                     DIKQKAVFGEASYDFGQFKLTAGGRYYDFKEKRDFISGGVFSNVDTRIGDKTKSDGFS
                     PRFIATYEPNRSLSVNLQAAKGFRLGGVNDPLNIPLCSGGATGPDAQTFGNRPTWDDE
                     TLWNYEAGLKYSRRGLTFNTAVFYTDIKDLQVTADAGSCSSRIVFNVPKAHTKGIEAE
                     LAASPMPGLDLSLAGSLIEAEFDSDVTTVGGAIIAGIREGNRLPTVPKFQMAATATYG
                     QRFTSNSDWYISGSVQHVGSRYTQPSDQEPGAGVFNFIFYDPNTGAFGTATQDFGSLK
                     LPAYELVNLSAGIEWDNGLELIAYVNNLFDENAKLSLDRERGGRARLGYNIGTPRTIG
                     LTLRKAFGIAPPPPPPPPPPPPPPPPPPPATQTCPDGSVILATDACPVPPPPPPPPPP
                     EPERG"
     gene            complement(4631..>7011)
                     /locus_tag="VFU20_01315"
     CDS             complement(4631..>7011)
                     /locus_tag="VFU20_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5285132.1"
                     /translation="YVPQNTISDASTFVRAPSLRGLPADEVLVQINGKRFNRSALVQV
                     FTGGDTGLSFGSQGADISAIPSIAIKNLQVLRDGATAQYGSDAIAGVMNFGMRNDRKG
                     IELQGRYGQFYDNGDTKLDSGISRQLAANVGVGIGATGFIDVAGEWYKDDGTSTGKTR
                     PIAVIFEQNNPSLADQLPHYPLPVQIWGSSPQHGWKGMLNSGVDVTTNSKLYLTVLGA
                     YNKGNQSFNYRSPISGTAVDVTGVTRTLGANGAFTNPFYLTPCPTGNATCPAGAFVQD
                     SNNFLFTSIYPAGFTPRFVGVTKERWGTLGWKGELGGGLTWDLSGTLAKNTLALSMYD
                     SLSPTYGPASQTSFEFGTLSQKERNANLDFTYPLDLGLFSPATLAWGAEYRKETYGQS
                     AGDEQSYGAGPFAAPQNIYVETAPGVFSFVRTIPGRQPGASGYGGTSPTYAGSWSQWN
                     WGIYGALEADVTEALSMGLAGRYEDYNTFGGSFVYKANGLYKVSPQFSVRATIGTGFH
                     APSPGQSNNAILTTNFVAGNQVQTGTFPVDSPISQYFGATSLKPEKSRNYGAGMIFKP
                     QSNLTFTVDGYIINVRGRIGVSQAFRVTAADIIAQPELASVGEGGDVNYFTNATNTST
                     KGIDMVGTWRTGLMGGDLSTTLAYNYNKSKVTKFDPAVISEAQRITAAHLAPNHRATL
                     SANYLRGPWSFNARENYYSWWQNELDYPGQKFGSRFTTDVDISYTFMEHYTATIGANN
                     LFNTRPEKIMHTDSNRIFVLTDSLADGQVYPRTGGPFGLNGGFWYARLKVKY"
BASE COUNT         1114 a         2203 c         2465 g         1229 t
ORIGIN      
        1 gcgcttgcct caatgcctgc ctcagcggtc gacgcaggga ccaaaggccc ggccgaaaaa
       61 ccagcccgca gtgctgccga caccaccggc gggcaaaccg agcccgttcc tagctcgccg
      121 cggatcggca tcgcccaatt cttcaccgac accgaccccc gcaccgggcg ccgctcgatg
      181 cgcgcatatg aggcggtgaa gacgcttgag cccgaccagc cgatcggctc gcagggcttc
      241 gatctggtcc tgtccgggcg cttgaaggcg cttcccggca agagcgtcat cgcctgcgtc
      301 gcccgaagcg ccgacagccc gcccgattgc atcgtttcgg ccgatttcga ccgggtgtgg
      361 atcgcggctc ccgccgatcg tgcgatcatc gcggagtggg gcggcggctg acccacttca
      421 atcgaccggg acgaggcgcc taatgcacct cgcgcttgtg ggtctcggcc ttggtggcaa
      481 ggctgaagcg cggctccggc tcgattgacc cgaccagcct cgcggcggcg tagcggccga
      541 cctcgggccc gtgcttgaag ccgtggcccg agccgtcgcc gaccagcagc acgtttggca
      601 gccgcgggtg gaaatcgatc aggaagtcgc cgttcgagct gttctcgtac tggcagaccc
      661 gcgcctcggt cagcggagcg cccctgagca gcgggaagcg gcggtcgcgg aaggcgacga
      721 tctcggccag cgcggctgcc gtcggccgcc ggtcctgggt gtcgggatcg accgcggccc
      781 cgtgcgcatc gtgggcgaat ttcactccac ggccctctag ctcggggaag ccataaaaga
      841 tgtcgccctc gttgaagtcg gcccagccgg gcatcgctgc gggcaggaat cggcggtcgc
      901 ccgcgggcgg ggcgaagaag aacacttcct ggcgagtcgg caggatccgg ccgccgatga
      961 cgtccgggaa cagcttgggc agccacgggc cgagtgcaaa cacgtaataa tcggcttgca
     1021 gggccgagcc gccgcgtaag cccaacgagg ccagccggcc gctttgctgc tgaacgacgt
     1081 gcgcggcctc atggcggtac tggccgccgc cgcggacgaa tcgctcgacc aaggtctgca
     1141 ccgagcggcg agccatcagc gccccgaagc cgggctcgaa caggccggcg ctcactccgg
     1201 tgaagtcgat catcgggaag cgccgcgaca tttccggacc ggtcaattcc tgcagcggca
     1261 ccccgagccg gcgatggacg gcgaccgaat cggcgaaata ggaatcgggc ttgctggaga
     1321 agaacagcac tccgtgcggc acgaagatcg gcaggccggc gagggcgctc agcgccttcc
     1381 actgcggcag gctgtcgaac gccatgcgcg tgtagacggc atccttgccg taggcggcgc
     1441 gggtcatgcg cgattcgccg cccgacgagg cgcggctgtg cgccggcccg tgcgcgtcga
     1501 tcagggtcac ccggtggccg gcggcaagca gatggtgggc ggtccaggcg ccgaacacgc
     1561 ccgcgccgac gacggcgacg tggcttttcg atcccagttg ggcggccctg gcgatggccg
     1621 gtgataaggc agcgccgccc gctgcggcaa gcagcgagcg gcgcgtcagg gagggaagcg
     1681 gcgcgcccga gggattgccg cttcccggat tcatcgcctc agccgatcag ccgcgctcgg
     1741 gctcgggcgg cggcggcggc ggaggcggcg gcggcaccgg gcaggcgtcc gtcgccagga
     1801 tcaccgagcc gtccgggcag gtctgcgtcg ccggaggcgg cggaggcggt ggcggcggag
     1861 gcggcggcgg gggcggcggg ggaggcggcg cgatcccgaa cgccttgcga agcgtcagcc
     1921 cgatcgtccg cggagtgccg atgttgtagc cgagccgggc acggccgccg cgctcgcggt
     1981 cgagcgacag cttggcattc tcgtcgaaca gattgttgac gtaggcaatg agctcgaggc
     2041 cgttgtccca ctcgatgccg gccgacagat tgaccagctc gtaggccggc agcttgagcg
     2101 aaccgaagtc ctgggtggcg gtcccgaacg cgcccgtgtt cgggtcgtag aagatgaagt
     2161 tgaacactcc cgcgcccggc tcctggtcgc tcggctgcgt gtagcggctg ccgacgtgct
     2221 ggacgctgcc cgatatgtac cagtcgctgt tcgacgtgaa acgctggccg taggtggccg
     2281 tcgccgccat ctggaacttg ggcaccgtcg gcaggcgatt gccttcgcga atgccggcga
     2341 tgatggcgcc tccgaccgtc gtcacgtcgc tgtcgaactc cgcctcgatg aggctgccgg
     2401 cgagcgacag gtcgagcccc ggcatcgggc ttgccgcaag ctccgcctcg atgcccttgg
     2461 tgtgcgcctt gggcacgttg aagacgattc gcgacgagca gctgccggca tcggcggtga
     2521 cctggaggtc cttgatgtcg gtgtagaaca ccgccgtgtt gaaggtcagg ccgcggcggg
     2581 aatatttgag gcccgcttca tagttccaca gagtctcgtc gtcccacgtc ggccggttgc
     2641 cgaacgtctg cgcgtcgggg ccggtggcgc cgcccgagca gagcgggatg ttcagagggt
     2701 cgttgacacc gccgagccgg aagcccttcg cggcctggag gttgacgctg aggctgcggt
     2761 tcggctcgta ggtcgcgatg aaacgcgggc tgaacccgtc ggacttggtc ttgtcgccga
     2821 tccttgtgtc gacattggag aagacgcctc cggagatgaa gtcgcgcttt tccttgaagt
     2881 cgtagtagcg cccgccggcg gtcagcttga actggccgaa gtcgtagctc gcctcgccga
     2941 acaccgcctt ctgcttgatg tcgtagggca ggtccgcgtt gtacggcgag ttcagcggga
     3001 agccgttcga gacggcggcc gatactccgg cgccgagcgc cgcatcgatg aacgcatcgt
     3061 agccgggggt cggcagccgc tgcgcatatt cacgatcgat gtcggtgtag aacacgccca
     3121 cgacccattg cagcgggccc gatcccgccg acgcgaggcg gacttcctgg gtccattgct
     3181 tcagatcggt cgtgtcgcgc aggttggacg ccaaattggc gatgtcgggg tcgactccgc
     3241 agcacccgac gagcggcacg atgccgaccg acccggtcag cgccgacgcg tcgcggctga
     3301 cgaggatgtc gcggtcgatg tagctggtga tcgaggtcag ctcgacgccc ccgaacccga
     3361 cgctggcggt caggtcggcg agcaatgtgt cgtccttgaa tttctcgcga agcttcaaat
     3421 actgcttgcg cttgccgact tccggctcgg tcgtgagcga cgtgtagaag ttgtagtgct
     3481 cctcgcggtt gaagccgtcg gtgcggatct gctgatagac cagccgcggc gtgatcttga
     3541 cgtcgggagc gggctcgaac agcagcgcga tgcggccgcc gatgcgggtg ccgtcgttga
     3601 tgttcttgcc ggcggcgggg ccgatcgcgt cgacgaagcc gccgtagcgg gtgccgtagc
     3661 cgaccagccg cgtcgcggcc gccgatccga gcgggaagtt gacgctgcct ttcacatggc
     3721 cgccgatgtc gccttcgtcg acggtgttga tattgccttc gaccatcccc tcggtggtgc
     3781 cgagctccgg ctggttggtg atgtagcgca gcgtcccgcc gaccgagccc gagccgaaca
     3841 gcgtgccctg cgggccgcgc agcgtctcga cgcggttcag atcgaacagg tcgaagtcgg
     3901 gcgtgaacag cgacagcgaa atgaccgatt cgtcgagata gacgccgacc tgctccttga
     3961 cgcccggctg gtcgcggacg atctggccgg ccgacacgcc gcgcaccgac acctggctct
     4021 ggcccggccc gaggttctgg accgtgaggc cggcgacgtt gcggctgatt tcctcgatgg
     4081 tcgaggcatt ggcgcgctgg atatcctcct gggtctgggc attgatcgag aaggggacgt
     4141 cctggacgcg cgacgcgcgc ttggtcgcgg tgacgatgat gtcctggccg gccgaagcct
     4201 cttcgggctg gttggcggcc tgggcgacgt cttcttcggt ggtctgggca aaaaccggcg
     4261 tcgaaatggc gatcgccgcg gtgccgatca gcagcagtgc cgatgtgcgc atgatgaatt
     4321 cccctgtttt ggccgcgtaa cggagcgtct ctacgcccaa gcgaggaatc ggcggaaggg
     4381 gcggcggcct ccgcgcgcat tttcgagccg ctcctgttgc aatagagtga caacctcggt
     4441 tgcgttatcc gaacggcagc gggcgcgctg aggagccgcg gcgagcttag gccgcgctcc
     4501 gccgggaact ggtggcggga gatttgcgct tgctgctcac gcccatggcg gccggatgga
     4561 cggcggtgcg cgcctcggca aaaaattggg cgggggcccg aagctccccg ccccttatcg
     4621 tcttacccgc tcaatatttg accttgagcc gggcatacca gaacccgccg ttgagcccga
     4681 aggggccgcc ggttcgagga tagacctggc cgtcggcaag gctgtcagtg agcacgaaga
     4741 tccggtttga gtcggtgtgc atgatttttt cggggcgggt gttgaacagg ttgttcgctc
     4801 cgatcgtcgc cgtgtaatgc tccatgaacg tgtagctgat atcgacgtcg gtggtgaacc
     4861 tcgagccgaa cttctggccc ggatagtcga gctcgttctg ccaccagctg taataattct
     4921 cgcgcgcgtt gaacgaccac ggaccgcgca agtaattggc cgacagcgtc gcgcggtggt
     4981 ttggagcgag gtgcgcggcc gttatcctct gagcctcact gatcacggcg ggatcgaact
     5041 tggtcacctt cgacttgttg taattgtacg ccagcgtggt cgacaaatcg ccgcccatca
     5101 gcccggtccg ccacgtcccg accatgtcga tgcccttggt cgaggtgttg gtggcgttgg
     5161 tgaagtaatt gacatcaccc ccttcaccaa ccgacgccag ctcgggctgc gcgatgatgt
     5221 cggctgccgt cacccggaag gcctgcgaga cgccgattcg gccgcgcacg ttgatgatgt
     5281 agccgtcgac ggtgaaggtc aggttggact ggggcttgaa gatcatgccc gcgccgtaat
     5341 tgcgcgactt ctccggcttg agcgaggtcg cgccgaaata ttgcgaaatc gggctgtcga
     5401 ccgggaaggt accggtctgc acctggttgc cggcgacgaa gttggtggtc aggatcgcgt
     5461 tgttggactg gccgggcgag ggtgcgtgga agccggtgcc gatcgtcgcc cgaaccgaga
     5521 attgcggaga caccttgtac aggccattgg ccttgtagac gaagctgccg ccgaaggtgt
     5581 tgtaatcctc gtagcggccg gcaagaccca tgctcagcgc ttcggtgaca tcggcctcga
     5641 gggcgccgta gatgccccag ttccactggc tccagcttcc cgcataggtt gggctggtgc
     5701 cgccgtaccc gctcgcgccg ggctgacggc ccggaatcgt gcgcacgaat gagaaaacgc
     5761 ccggggcggt ttccacatag atgttctgcg gcgccgcgaa cgggcccgcg ccataggatt
     5821 gctcgtcacc cgcggactgg ccgtaggtct ccttgcgata ctccgcgccc caggccagcg
     5881 tggccgggct gaagagcccg aggtccagcg gataggtgaa gtcgaggttg gcgttgcgtt
     5941 ccttctggct cagcgtgccg aattcgaagc tggtctgcga ggccggcccg tacgttgggc
     6001 tcaaggaatc gtacatcgac aaggcgagcg tgttcttggc gagcgttccg gacaggtccc
     6061 aggtgagccc gccgccgagc tcgcccttcc agccaagcgt gccccagcgc tccttggtga
     6121 cgccgacgaa gcggggcgtg aagccggccg ggtagatgga ggtgaacagg aaattgttgc
     6181 tgtcctgcac gaacgcgccg gccgggcagg tcgcgttccc ggtcgggcag ggcgtcagat
     6241 agaaggggtt ggtgaaggct ccattggcgc ccagggtgcg cgtcacgccg gtcacgtcga
     6301 cggcggttcc gctgatcggc gagcggtagt tgaagctctg gttccccttg ttgtaggcgc
     6361 cgagcaccgt caaatagagc ttgctgttgg tcgtgacgtc gacgccgctg ttgagcatgc
     6421 ccttccagcc gtgctggggc gacgaacccc agatctgaac cggcagcggg taatgcggca
     6481 actggtccgc gaggctcgga ttgttctgct cgaagatgac cgcgatcggc cgggtcttgc
     6541 cggtgctggt gccgtcatcc ttgtaccatt cgccggcgac gtcgatgaac ccggttgccc
     6601 cgatcccgac accgacgttg gccgcgagct ggcggctgat tccgctgtcg agcttggtgt
     6661 cgccattgtc gtagaactgg ccgtaacggc cctgaagctc gatgcccttg cggtcattgc
     6721 gcatgccgaa gttcatgacc ccggcgatcg cgtccgagcc gtattgcgcg gtcgcgccgt
     6781 cgcgcagcac ctggaggttc ttgatcgcga tcgaagggat cgccgaaatg tcggcgccct
     6841 gcgagccgaa cgagaggccc gtgtcgccgc cggtgaagac ctggaccaag gccgaccggt
     6901 tgaagcgctt gccgttgatc tggaccagca cttcgtcggc cggcaatccg cgcagcgaag
     6961 gcgcgcgaac gaaggtcgaa gcgtccgaaa tggtgttctg cggcacgtag a
//
LOCUS       DASTZO010000050         5968 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_265886,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000050 DASTZO010000000
VERSION     DASTZO010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5968)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5968)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5968
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_265886"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VFU20_01320"
     CDS             <1..333
                     /locus_tag="VFU20_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010410278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEU5285133.1"
                     /translation="PIMFLIGVPWAEAGTAGGLFGTKLVLNEFVAFIDLGQMSAAAMS
                     ERSRAIVTFALCGFANFSSIAIQMAVTGGLAPNQRPVIARLGIRALIAGSLANLMSAA
                     LAGLLISA"
     gene            344..1291
                     /locus_tag="VFU20_01325"
     CDS             344..1291
                     /locus_tag="VFU20_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate and iron-dependent oxygenase
                     domain-containing protein"
                     /protein_id="HEU5285134.1"
                     /translation="MATITADSVASVSLNDADREPDTFAGKLGRSFEEYGFAIIADHG
                     IPDELIRDAEEKAKAFFALPDEVKRKYHVPGGGGARGYTPFGIETAKGAQAFDLKEFW
                     HVGRELPPGHPFRSHMGDNIWPEEIASFRDTFLTLYDTFDRTGLKILKAIARFLKIDE
                     DYFIDTVRDGNSVLRLLHYPPIEGEPGSNVRAGAHEDINTITLLLGAEEAGLELLTRD
                     GRWIPVSPKPGELVVNIGDMLQRLTNGLLRSTTHRVVNPPPERRGRSRYSMPFFLHFR
                     SDFCIEALPGTVPPGEQPKEPPITANDYLQERLREIKLT"
     gene            1389..1715
                     /locus_tag="VFU20_01330"
     CDS             1389..1715
                     /locus_tag="VFU20_01330"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5285135.1"
                     /translation="MGNDLDSGIPSDRPSMRAREIRTSLDAEVSLRRSGQHHYKVRIY
                     NASPHGCRIEFVERPSLDEHVWVRFEGLEAIEGQVCWVDGFVTGVEFVKPIHPAVFDR
                     LVGGKP"
     gene            complement(1742..2191)
                     /locus_tag="VFU20_01335"
     CDS             complement(1742..2191)
                     /locus_tag="VFU20_01335"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU5285136.1"
                     /translation="MTEEELVEAARGAALRAYAPYSRFHVGCAIESAAGEVVTGANME
                     NACYRLGICAEQSALTAAQAAFGLEQVARVAVAGGRIEDGALAGDGTVTPCGGCRQAI
                     LEAAQLSGRDVEILCASGDGGVVERHTVATLIPHGFGPANLEDGNPA"
     gene            2251..3063
                     /locus_tag="VFU20_01340"
     CDS             2251..3063
                     /locus_tag="VFU20_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1/P1 nuclease"
                     /protein_id="HEU5285137.1"
                     /translation="MKLRPARLVILAAALAAIMSPSPASAWGRTGHRVIAAVADRHLS
                     GLARAHVREILGVESLDEAAYWPDEMKSDPSPFWQKTASPWHYVTVGGFTYDRPPPEG
                     DAQSALAKFRAILLDRSASKADRQLALRFIVHLVADLHQPLHVGRPGDRGGNEVKVTW
                     FGRETNLHAVWDSALVDDEQLSFTEWADRLGRHTSSRDVIDWWVVNPMVWITESAQVR
                     ETLYPERRDLSYEYVFRHKPAVKRRLAQGGVRLAAYLNDLFDEASEPPPNPQ"
     gene            3060..4748
                     /locus_tag="VFU20_01345"
     CDS             3060..4748
                     /locus_tag="VFU20_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HEU5285138.1"
                     /translation="MKPPLFALALALAACASAPRPPTLPAAPVEVQILAINDFHGNLE
                     PPASPIQRVEPDGSKLREALGGGAFLAAALAELRQGRRNSLTVSAGDLIGASPLISAN
                     FLDEPTVLAMNGMGLDFNAVGNHEFDKGAAELLRIQNGGCEKFTTREPCQVDRPFAGA
                     RFRLLAANVLKPDGSTLFPATAIRDFGTVQVGIIGMTLRETATLVAPAGVAGLRFTDE
                     AATANALVPKLKAEGADLIVLAIHQGGRSDGEFNVVGCPGLRGDILPILDSLDPAVSV
                     VVSGHTHHAYACQRPARDGSPRLLTSAGRYGYFVTDIRVQVDPALGRWVRAAAVNVPV
                     TRARAPDPKLKLLVDRYAAAAAPAAARIVGRLSGPAMHSLFDDETEAANLIADAQLAA
                     SRSPRAGGAVVAFMNGAGVRTDLVPDSRGNVRFGQIFDMQPFGNSLVVMSLTGEQIRR
                     MLEQQFRADSYPPAARPALLVPSAGFRFEYDLSRPSGKRIVAMTLHCRPLDPARSYRV
                     TVNNFLASGGDGFTVLTEGRDRFDAGPDLDALEALLGKGATVPRLGRTRNVTPR"
     gene            complement(4745..>5968)
                     /locus_tag="VFU20_01350"
     CDS             complement(4745..>5968)
                     /locus_tag="VFU20_01350"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEU5285139.1"
                     /translation="PGIYATSIARPALMKEYLRVQLEHLVHDYAVDITVGRSASEIPY
                     PYVLDGAEIELGEVGASELSRWFPSSELVHIGDEIADGAWDLSRHTARPLALFDGPRT
                     DFSLARLRHYTGTPPEHFQHFVLFTNYVRYVDEFVAFALGELKRPDSPFAAFSVPGGL
                     YSAGDLADGEGRIGAGDWRRNQMPAYHLMAEGGTGITLVNIGVGPSNAKTICDHIAVL
                     RPEVWLMIGHCGGLRPSQAIGDYVLAHAYLRDDHLLDDVLPVEIPLPAIAEVQRALFE
                     AATLVTGEDEDSLKKRLRTGTVVTTDDRNWELRYTLSALRFNQSRAVAIDMESATVAG
                     QGYRFRVPYGTLLCVSDKPLHGELKLPGQANAFYDRAISQHLRIGIEALLLLKREGDA
                     LHSRKLRSFDEPPLR"
BASE COUNT          958 a         2107 c         1976 g          927 t
ORIGIN      
        1 ccgatcatgt tcctgatcgg agtgccgtgg gccgaagccg gcaccgccgg cggcctgttc
       61 ggcaccaagc tggtgctgaa cgagttcgtc gccttcatcg acctcgggca gatgagcgcc
      121 gcggcgatga gcgagcgcag ccgcgccatc gtcaccttcg ccctgtgcgg gttcgccaat
      181 ttcagctcga tcgccatcca gatggcggtg accggcggcc tcgcccccaa ccagcggccg
      241 gtgatcgccc ggctcgggat ccgggcgctg atcgccggca gccttgccaa cctgatgagc
      301 gcggcgctgg cgggattgct gatttcggcc taaaacccgt cgcatggcca ccatcaccgc
      361 agacagtgtc gcgtccgttt cgctcaacga cgccgaccgc gagcccgaca cgttcgccgg
      421 caagctcggc cgcagcttcg aggaatacgg cttcgcgatc atcgccgacc acggcatccc
      481 cgacgagctg atccgcgacg ccgaggagaa ggcgaaggcc tttttcgctc tgcccgacga
      541 ggtgaagcgc aaataccatg tcccgggcgg cggcggggcg cggggctaca cgccgttcgg
      601 catcgagacc gccaagggcg cccaggcgtt cgacctcaag gaattctggc acgtcggccg
      661 cgagcttccg cccggccatc cgttccgcag ccatatgggc gacaatatct ggcccgagga
      721 aatcgccagc ttccgcgaca ccttcctcac cctctacgac acgttcgacc gcaccggcct
      781 caagatcctg aaagccatcg cccgcttcct gaagatcgac gaggattatt tcatcgacac
      841 cgtccgcgac ggcaattcgg tgctccgcct gctgcactac ccgccgatcg agggcgagcc
      901 cggaagcaac gtccgcgccg gagcgcacga ggacatcaac accatcacgc tcctgctcgg
      961 cgccgaggag gccgggctcg agctcttgac ccgcgacggg cgctggatcc cggtgtcgcc
     1021 caagcccggc gagctggtgg tgaacatcgg tgacatgctc cagcggctga ccaacggcct
     1081 gctgcgctcg accacgcacc gggtcgtcaa cccgccgccc gagcgccgcg gccgctcgcg
     1141 ctactcgatg cccttcttcc tccacttccg atcggatttc tgcatcgagg cgctgccggg
     1201 caccgtcccg cccggcgagc agcccaagga gccgccgatc accgccaatg actatctcca
     1261 ggagcggctg cgcgagatca agctgacctg agttggcgcg cgaaagccta tccacaacgg
     1321 agtcggttgc cttgtaaagt ttctcgggca gactcatgag tgtcggatcg gcttgagcgg
     1381 gcgcggctat gggaaacgac ctggacagcg ggattccttc ggaccggccg tcgatgcgcg
     1441 cgcgggaaat ccgcacctcc ctcgacgccg aggtctccct tcgccgatcc ggccagcacc
     1501 attacaaggt caggatctac aacgcgtcgc cgcacggctg caggatcgag ttcgtcgagc
     1561 gacccagcct cgatgagcat gtctgggtcc ggttcgaggg gctggaagcg atcgagggcc
     1621 aagtctgctg ggtcgacggc ttcgtcaccg gcgtcgagtt cgtgaagccc atccacccgg
     1681 cggtgttcga ccggctcgtc gggggcaagc catagcgctg ctcccgcgcg ggcaccatca
     1741 cctaggccgg attgccgtcc tccagattgg cggggccgaa gccgtggggg atgagcgtcg
     1801 cgacggtgtg gcgctcgacg acgccgccgt cgccgctggc gcacaggatc tcgacatcgc
     1861 ggccggacag ctgcgccgct tccaggatcg cctggcggca gccgccgcag ggcgtgacgg
     1921 tgccgtcccc cgccagcgcg ccatcctcga tccgcccacc ggcgaccgcg acccgggcga
     1981 cctgctcgag cccgaacgcg gcctgcgcgg cggtcagagc gctctgctcg gcgcagatgc
     2041 cgagccggta gcaggcgttc tccatattgg ccccggtcac cacttcgccg gcggccgatt
     2101 cgatcgcgca gccgacatgg aaccgcgaat aaggcgcata ggcccgcagc gccgcgccgc
     2161 gcgcggcctc gacaagctct tcctctgtca tcaaactgac cttaggcggg ttcagaagcg
     2221 ctcgacaacc agtctcgcag gagtggcgtc atgaagttgc gtccggcccg gctcgttatc
     2281 cttgcagcgg cgctcgccgc gatcatgagc ccgagcccgg cgtccgcctg gggccgcacc
     2341 ggccaccggg tgatcgcggc ggtcgccgac cggcacctga gcggcctcgc ccgagcgcat
     2401 gttcgcgaga ttttgggcgt cgaaagcctc gacgaggccg cctactggcc cgacgagatg
     2461 aagtccgacc cgagcccttt ctggcagaag accgcctcgc cctggcatta tgtgaccgtc
     2521 ggaggcttca cctatgaccg gccgccgccc gagggggacg cgcagtcggc cctcgcgaaa
     2581 ttccgcgcga ttctgctgga ccggtcggcg tcgaaggccg accgccagct ggcgctgcgc
     2641 ttcatcgtcc acctggtcgc cgacctgcac cagcccctgc acgtcggccg ccccggcgac
     2701 cgcggcggaa acgaggtgaa ggtgacctgg ttcgggcgcg agaccaatct tcacgcggtg
     2761 tgggattcgg cgctggtcga cgacgagcaa ttgtccttta ccgaatgggc cgatcggctc
     2821 ggccgccaca ccagcagccg cgacgtcatc gactggtggg tggtcaaccc gatggtctgg
     2881 atcaccgaga gcgcccaggt ccgcgagacc ctttatcccg aacggcgcga cctgtcctac
     2941 gagtatgttt ttcgccacaa gcctgccgtc aagcgccgcc tggcgcaggg cggcgtgagg
     3001 ctcgcggcct atctcaacga tctgttcgac gaagcgagcg aaccgccgcc caacccgcaa
     3061 tgaaaccgcc gctcttcgcc ttggcgcttg ccctcgcggc gtgcgcttcc gcgcctcgac
     3121 cgccgacgct tccggccgcg ccggtcgagg tgcaaatcct cgcgatcaac gatttccacg
     3181 gcaacctcga gccgccggca tcgccgatcc agcgagtcga gccggacggg tcgaagctcc
     3241 gcgaggcgct gggcggcggc gcctttctcg ccgcggcgct cgccgagctt cggcaggggc
     3301 ggaggaacag cctgaccgtt tcggccggcg acctgatcgg agcgagcccg ctgatctccg
     3361 ccaatttcct cgacgagccg acggtgctgg cgatgaacgg catgggcctc gacttcaacg
     3421 cggtcggcaa tcatgaattc gacaagggcg ccgccgagct gctgcggatc caaaatggcg
     3481 gctgcgaaaa attcaccacc cgggagccgt gccaggtcga ccgcccgttc gctggggccc
     3541 gcttccggtt gctcgccgcc aatgtcctga aacccgacgg atcgacgctg tttccggcga
     3601 ccgcgatccg ggacttcgga acggtgcagg tggggattat cggcatgacc ctcagggaga
     3661 ccgcgacgct ggtggccccc gccggagtgg ccggcctgcg cttcaccgac gaggcggcga
     3721 ctgcgaacgc gctggttccg aagctcaagg ccgaaggcgc cgacctgatc gtgcttgcca
     3781 ttcaccaggg tggccgctcg gacggcgagt tcaacgtcgt cggctgcccc gggctcagag
     3841 gcgacatcct gccgatcctc gactcgctcg atcccgccgt atcggtggtc gtgtcgggcc
     3901 acacgcacca cgcctatgcc tgccaacggc cggcccggga cggcagcccg cgacttctga
     3961 ccagcgccgg acgatacggc tatttcgtca ccgacatccg ggtccaggtg gatccggcgc
     4021 tgggccgctg ggtccgagcg gcggccgtca acgtgccggt gacccgcgcc cgggcccccg
     4081 acccgaagct gaagttgctg gtggaccgct acgccgctgc ggccgcgccg gccgccgccc
     4141 ggatcgtagg ccggctttca ggcccggcaa tgcacagcct gttcgacgac gagaccgagg
     4201 cggccaacct gatcgccgac gcccagctcg ccgccagccg gtcgccccgg gcgggcgggg
     4261 cggtggtcgc cttcatgaac ggggccgggg tccgcaccga cctggttccc gactcccggg
     4321 gcaatgtccg attcggccag attttcgaca tgcagccgtt cggcaacagc ctggtcgtga
     4381 tgtcgctgac cggagagcag atccgaagga tgctcgaaca gcagttccgg gccgattcct
     4441 atccgcccgc cgcgaggccg gcgctgctgg tgccgtcggc aggcttccgc ttcgaatatg
     4501 acctgtcgcg gcccagcggc aagcgcatcg tcgcgatgac gctccattgt cgcccgctcg
     4561 acccggccag gagctaccgg gtcacggtca acaatttcct agccagcggc ggtgacggct
     4621 tcaccgtcct caccgaaggc cgggaccggt tcgacgccgg tccggacctg gatgcgctcg
     4681 aggcgctgct cggcaagggg gccacggtcc cccgcctcgg ccgcacccgc aacgtcacgc
     4741 cgcgctagcg cagcggcggc tcgtcgaagc tgcgcaactt gcggctgtga agcgcgtcgc
     4801 cctcgcgctt caagagcagc agcgcctcga tgccgatgcg caaatgctgg ctgatcgccc
     4861 ggtcgtagaa ggcgttggcc tggccgggca gcttgagctc gccgtggagc ggcttgtcgg
     4921 acacgcacag cagcgtcccg tacggcaccc ggaagcggta gccctggccg gcgacggtcg
     4981 ccgattccat gtcgatcgcc accgcccggc tctggttgaa gcgcagcgcc gacaatgtgt
     5041 agcgaagctc ccagttgcgg tcgtcggtgg tgaccacggt gccggtccgg agccgcttct
     5101 tcaacgaatc ctcgtcctcg ccggtgacca gcgtcgcggc ctcgaacaaa gcccgctgga
     5161 cctcggcgat cgccggcagc gggatttcca ccggcagcac gtcgtcgagc agatgatcgt
     5221 cgcgaagata ggcgtgggcg agcacatagt cgccgatcgc ctggctgggc ctcaaaccgc
     5281 cgcaatggcc gatcatcagc cacacctcgg gccggagcac cgcgatatgg tcgcagatcg
     5341 tcttggcgtt ggacgggccg acgccgatgt tgaccaaggt gatgccggtg ccgccctcgg
     5401 ccatcaggtg ataggcgggc atctggttgc ggcgccagtc gccggcgccg atccgccctt
     5461 ccccatcggc gaggtcgccg gcggaataga gaccgccggg caccgaaaag gcggcgaacg
     5521 ggctgtccgg ccgcttgagc tcgccgagag cgaaggcgac gaactcgtcg acgtagcgca
     5581 catagttggt gaacagcacg aagtgctgga aatgctcggg cggagtgccg gtgtagtggc
     5641 gcagccgagc caggctgaag tcggtgcgcg gcccgtcgaa cagcgccagc gggcgcgcgg
     5701 tgtgccgaga caggtcccac gcgccgtcgg cgatctcgtc gccgatgtgg accagctcgc
     5761 tcgacgggaa ccagcgcgac agctcggacg cgccgacctc gcccagttcg atctcggccc
     5821 cgtcgagcac ataagggtaa gggatttcgc tggccgaccg gccgaccgtg atgtcgaccg
     5881 cataatcgtg gaccagatgc tcgagctgga cgcgcagata ttccttcatc agcgccggcc
     5941 gggcgatgct ggtcgcgtag atgccggg
//
LOCUS       DASTZO010000051         4477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1153709,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000051 DASTZO010000000
VERSION     DASTZO010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153709"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VFU20_01355"
     CDS             <1..300
                     /locus_tag="VFU20_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEU5285140.1"
                     /translation="IAGLMDLGGGVALRVVSDGLDALRGALAHDLHGLLGAQDSGGWR
                     PHVTIQNKVAPKAARALLRSLEDDFRPRPLRIAGLGLHRYLGGPWETLGVWKFRG"
     gene            complement(297..722)
                     /locus_tag="VFU20_01360"
     CDS             complement(297..722)
                     /locus_tag="VFU20_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HEU5285141.1"
                     /translation="MDPLTPLDTLCRIILRAREYEAQTPTDYDGGEAADNVDDEDEGA
                     LSVLDDTINDSVEEELKAILEDLGEDQLAEVVAFSWVGQGTYDTADWDEAMEEASALA
                     AGGGESAIDELLDMPMLASVLEAGLAAFELSCDGIGDVG"
     gene            902..978
                     /locus_tag="VFU20_01365"
     tRNA            902..978
                     /locus_tag="VFU20_01365"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:936..938,aa:Met,seq:cat)
     gene            complement(1004..1234)
                     /locus_tag="VFU20_01370"
     CDS             complement(1004..1234)
                     /locus_tag="VFU20_01370"
                     /inference="COORDINATES: protein motif:HMM:NF041742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGxxGxxG family protein"
                     /protein_id="HEU5285142.1"
                     /translation="MRTKLMLAALLAATAAGPPALAQNQDVGGIPTETQRRLAQGDVG
                     VPWDLIGLFGLLGLLGLRKRHPDDSYHPAPLE"
     gene            1727..3049
                     /locus_tag="VFU20_01375"
     CDS             1727..3049
                     /locus_tag="VFU20_01375"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU5285143.1"
                     /translation="MRVSRCFLPVLKESPSDAQIVSHRLMLRAGLVRQTAAGIYAWLP
                     IGFRVLKKIEQIVREEQDRAGAIEMLMPTIQPADLWKESGRYDAYGPEMLRIRDRHDR
                     EMLYGPTNEEMLTAIFRDDVKSYRDLPRTLYHVQWKFRDEVRPRFGVMRGREFLMKDA
                     YSFDLDEAGARASYYTQLLAYLRTFQRMGIQAVPMKAASGPIGGDLSHEFIVLAPTGE
                     SEVFYDSAFEDIDWNRGDLDYADEAGLHGLFEQVSSTYAATDETHDEARWAGLPEDRR
                     RTGRGIEVGHIFYFGDKYSAAMGLKVSGADGRPIHPMMGSYGVGVSRLVGAIIEASHD
                     EAGIIWPEAVAPWRVGIVTMRADDEPTAAAADRLYSDLTAAGVETLYDDRDERGGVKL
                     TSMDLIGLPWQLILGPRGIACGTLELKNRRTGEREELSVESALARLAA"
     gene            3046..4296
                     /locus_tag="VFU20_01380"
     CDS             3046..4296
                     /locus_tag="VFU20_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HEU5285144.1"
                     /translation="MILNRYERMIARRYLLPGKGEGFIFLVAAISLMAVALGVAALII
                     VMSVMNGFRGELFDKIVGLNGHAIVQGYEGRLSNWQQIAEAARKTHGVTSALPLIEQP
                     LMASANGRVEGVLVRGVRVEDLRSNRTITENVKSGDIRLITPGSNRIAIGSRLAEMLG
                     AFPGSEIALISPEGRSTVVGTVPRIVSYTVGATFEVGVYDYDKAFVVMPMEDAQTLLM
                     MGDQVGMIEIQSTDPDRVGEILAPLEPLVRGRGIIVDWRQMNSALFEALEIERIAMFV
                     VLSLIVLVAVFNILSSLIMLVRAKTRDIAILRTMGASRQGMMKVFVTVGVTIGSLGIV
                     LGLILGAIFLFFRQGVVDAIQAMTGQNLWDPSVRFLSELPSKTDPFEVAAIVITALVL
                     SFLATLYPAWKAASTDPVQVLRYE"
     gene            4289..>4477
                     /locus_tag="VFU20_01385"
     CDS             4289..>4477
                     /locus_tag="VFU20_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5285145.1"
                     /translation="MSDAVLTTRGLERSFTQADVTIEVLRGVDLEVRPGEIVALLGPS
                     GSGKSTLLQAVGLLEGGFS"
BASE COUNT          687 a         1549 c         1500 g          741 t
ORIGIN      
        1 atcgccgggc tgatggacct tggcggcgga gtcgctttgc gggtcgtgtc cgacggtctc
       61 gatgcgcttc gcggcgcgct cgcccacgat ctccacgggt tgctcggagc gcaggattcg
      121 ggcggctggc ggccgcatgt caccatccag aacaaggtcg cgcccaaggc ggcgcgcgct
      181 ctgctccgct cgctcgagga cgattttcgg ccgcggccgc tgcgaatcgc cgggctcggg
      241 ctccaccgct acctcggcgg gccgtgggag acgctcgggg tctggaagtt ccgcggctag
      301 ccgacgtcgc cgatcccgtc gcagctcaat tcgaacgcgg cgagccccgc ctcgagcacc
      361 gaggccagca tcggcatgtc gagcagttcg tcgatcgccg actcgccgcc gccggcggca
      421 agcgcgctcg cttcctccat cgcctcgtcc cagtcggcgg tatcgtaggt gccctggccg
      481 acccaggaga aagcgaccac ttctgccaat tggtcctcgc cgagatcctc gaggatcgcc
      541 ttcagttcct cctcgacgct gtcgttgatg gtgtcgtcga gcaccgacag agcgccctcg
      601 tcctcgtcgt cgacattgtc cgccgcctcg ccgccgtcat agtcggtcgg ggtctgggcc
      661 tcatattcgc gcgctcgaag gatgatccgg cacaaagtat cgagcggggt cagcgggtcc
      721 attcaagcgc ctccatacgc tcggctcaac gagggcaggg gtaagcggtt cctcgcgttg
      781 accggcggca tggcgccacc tatatccgcc gccgctcccg ccgcggcggg cgcggcccaa
      841 agcctccgcg gaaatgccgc ggcttgcctt ggccggcagc gattgggccg gcagagtttg
      901 gggcggagta gctcagctgg ttagagcagc ggaatcataa tccgcgtgtc gggggttcaa
      961 gtccctcctc cgctaccacc ttccatcccc ggttttctta ccgctactcg agcggggccg
     1021 ggtgatagct gtcgtcgggg tgccgcttgc gcaggcccag cagcccgagc aggccgaaca
     1081 gcccgatcag gtcccacggg actccaacat ccccctgggc cagccgccgc tgcgtttcgg
     1141 tcgggatccc gcccacatcc tggttctggg cgagcgccgg cgggccggcc gcggtcgccg
     1201 cgagaagagc cgcgagcatc agcttggtgc gcatgtccgt cctccctgtt cgcattcgcg
     1261 tgatttgcgg cgaagacgca aaaccgcaac ggattggacg cggaactgtt ccgcaggtcg
     1321 aacgacttct cggaaggcca aaagaaaacg cccggaccga caagcggtcc gccttgaccg
     1381 cgacaaggaa aacgcccgga ccgacgggcg gtccgggcgt cctgcgtgac gattgaaaaa
     1441 atcgtcagcc cccttcatgc gatcgaccgg cgcccgacgg gcccgatcgc ctccccgaac
     1501 ccggctgttg gaaagccgct gcccggaact gttgaaacgc tagtcccgca ttcgctcgct
     1561 tgtcacctgc gcactgccgg ccgggcggtt tttccccggc ccctgtgtcg gtcgagacac
     1621 agcgccgccc ggcccgccgc ggcccgcgag cgcattgcct tggccaaccc gccgccctag
     1681 gggaagcgcc gcagccaacc ctccacagcc ggaaggcgtc cacactttgc gagtctcccg
     1741 ctgtttcctg ccggtcctca aggaaagccc gtcggacgcc cagatcgtca gccaccggct
     1801 gatgctgcgc gccgggctgg tccgccagac cgccgccggc atctacgcct ggctgccgat
     1861 cggcttccgg gtcttgaaga agatcgagca gatcgtccgc gaggagcagg accgcgccgg
     1921 cgccatcgag atgctgatgc cgaccatcca gccggccgac ctttggaagg agagcggccg
     1981 ctacgacgcc tacgggcccg agatgctccg gatccgcgac cgccacgacc gcgagatgct
     2041 ctacggcccg accaacgagg agatgctgac cgcgattttc cgcgacgacg tcaagagcta
     2101 ccgcgacctg ccgcgcacgc tctaccacgt ccagtggaag ttccgcgacg aggtgcgccc
     2161 gcgcttcggg gtgatgcgcg gccgcgagtt tttgatgaag gacgcttatt cgttcgacct
     2221 cgacgaggcc ggcgcgcgcg cgagctacta cacgcaattg ctcgcctacc tgcgcacctt
     2281 ccagcggatg ggcatccagg cggtgccgat gaaggcggcg tcggggccga tcggcggcga
     2341 cctcagccac gagttcatcg tcctcgcccc gaccggcgag agcgaggtgt tctacgactc
     2401 cgcgttcgag gacatcgact ggaaccgcgg cgacctcgac tatgccgacg aagccgggct
     2461 ccacggcctg ttcgagcagg tgagctcgac ttacgccgcg accgacgaga cccacgacga
     2521 ggcgcgctgg gccgggcttc ccgaggaccg ccgccgcacc ggccgcggaa tcgaggtcgg
     2581 ccacatcttc tatttcggcg acaaatattc cgctgcgatg ggtttgaagg tgtcgggcgc
     2641 cgacggacgg ccgatccacc cgatgatggg cagctacggc gtcggagtgt cgcggctggt
     2701 cggcgccatc atcgaggcca gccacgacga ggcggggatc atctggcccg aggcggtcgc
     2761 cccgtggcgg gtcggcatcg tcaccatgcg cgccgacgac gagccgaccg ccgccgccgc
     2821 cgaccggctc tactccgact tgaccgcggc cggagtcgag acgctgtacg acgaccgcga
     2881 cgagcgcggc ggggtcaagc tcacctcgat ggacctgatc ggcctgccgt ggcagctgat
     2941 cctcggcccg cgcgggatcg cctgcggcac gctcgagctc aagaaccgcc gcaccggcga
     3001 gcgcgaggag ttgagcgtcg agagcgcgct ggcgaggctg gccgcatgat cctcaaccgc
     3061 tacgagcgga tgatcgctcg gcgctacctg ctcccgggca agggcgaggg gttcatcttc
     3121 ctggtcgcgg cgatcagcct gatggcggtc gcgctcgggg tcgcggccct catcatcgtc
     3181 atgagcgtga tgaacggctt tcgcggcgag ctgttcgaca agatcgtcgg cttgaacggc
     3241 catgccatcg tccagggcta cgagggccgg ctgtccaact ggcagcagat cgcggaggcg
     3301 gcgcgcaaga cccacggcgt gacgtccgcg ctgccgctga tcgagcagcc gctgatggcc
     3361 tcggccaatg gccgggtcga gggcgtgctg gtgcgcggcg tgcgcgtcga ggacctgcgc
     3421 tccaaccgga ccatcaccga gaacgtcaag tccggcgaca tccggctgat caccccgggc
     3481 agcaaccgga tcgcgatcgg ctcgcggctg gccgagatgc tcggcgcctt tccgggcagc
     3541 gagatcgccc tgatcagccc cgaaggccgc tcgaccgtcg tcggcacggt gccgaggatc
     3601 gtctcctaca ccgttggcgc gacgttcgag gtcggggtct acgattacga caaggcgttc
     3661 gtcgtcatgc cgatggagga cgcccagacg ctgctgatga tgggcgacca ggtcggcatg
     3721 atcgagatcc agtcgaccga ccccgaccgg gtcggcgaga tcctcgcgcc gctcgagccc
     3781 ttggtccgcg gccgcggcat catcgtcgac tggcggcaga tgaactcggc cctgttcgag
     3841 gcgctggaga tcgagcgcat cgcgatgttc gtggtgcttt cgctcatcgt gcttgtggcg
     3901 gtgttcaaca tcttgtcgtc gctgatcatg ctggtccgcg ccaagacccg cgacatcgcc
     3961 atcctccgga ccatgggcgc cagccggcag gggatgatga aggtgttcgt caccgtcggg
     4021 gtgacgatcg gctcgctggg catcgtcctc gggctgatcc tgggcgcgat cttcctgttc
     4081 tttcgccagg gcgtggtcga cgcgatccag gcgatgaccg gccagaatct gtgggacccg
     4141 tcggtccgct tcctgtccga attgccgtcc aagaccgacc cgttcgaggt cgccgcgatc
     4201 gtcattaccg cgctggtcct gtcgttcctg gcgacgctct acccggcgtg gaaggcggcg
     4261 agcaccgacc cggtccaggt gctgcgctat gagtgacgcg gttctgacca cccgcggcct
     4321 cgagcgcagc ttcacccagg ccgacgtcac catcgaggtg ctgcgcgggg tcgacctcga
     4381 ggtgcggccc ggcgagatcg tcgccttgct cggtccgtcg ggctcgggca agtcgacgct
     4441 tctgcaggcg gtcgggctgc tcgagggcgg cttttca
//
LOCUS       DASTZO010000052         2157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1231926,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000052 DASTZO010000000
VERSION     DASTZO010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2157
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231926"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFU20_01390"
     CDS             <1..346
                     /locus_tag="VFU20_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5285146.1"
                     /translation="IAMDIETIVRDLGHNVTGVAVTRDEAVAQARSNPPGLVLADIQL
                     ADDSSGIDAVKDILHEFKVPVIFITAFPERLLTGTRPEPTFLITKPFQRSTVKAAIAQ
                     ALFFDAATVPAA"
     gene            452..592
                     /locus_tag="VFU20_01395"
     CDS             452..592
                     /locus_tag="VFU20_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285147.1"
                     /translation="MAEDDQQHVTTTEARAGATPHMTRYVLAIGTGLVIVLFAIILFV
                     AG"
     gene            713..1318
                     /locus_tag="VFU20_01400"
     CDS             713..1318
                     /locus_tag="VFU20_01400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5285148.1"
                     /translation="MDARANPVPLSDPEFKDQLGAVIPHLRAFGRSLSGSRDLADDLV
                     QETLLKAWAARKRFQAGTNMRAWTFIILRNLFLSQMRRARFKGEWDEITAARILAAPA
                     SQDRHIELGDMQRALMHLPQPQREALILVGAGGFAYEEAAEICGCAVGTIKSRVARGR
                     VALETLLSSGKMPSRRQHRSDPNKSALQMIMGQVDELSRDR"
     gene            complement(1323..1460)
                     /locus_tag="VFU20_01405"
     CDS             complement(1323..1460)
                     /locus_tag="VFU20_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285149.1"
                     /translation="MDRRIIRVDIPPAHAGITAALRRAFEAAAVEPCDRDFAELLRQL
                     N"
     gene            1571..>2157
                     /locus_tag="VFU20_01410"
     CDS             1571..>2157
                     /locus_tag="VFU20_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF294 nucleotidyltransferase-like
                     domain-containing protein"
                     /protein_id="HEU5285150.1"
                     /translation="MTRAIGSDSRNGAMERARRFSPFLREALEGRPELGDCFLEQGSR
                     AAVAAALAEGDGPVEVELRRARLGLALTIALGDLAGELGLEEVTRTLSDFADQAIGRA
                     LAAAIGERVADAGPTGFAVIALGKLGSRELNYSSDVDLLLLFDPIALPRRERDDAGEA
                     AVRVARRMIEILQKRTADGYVARVDLRLRPSPEVTP"
BASE COUNT          343 a          719 c          768 g          327 t
ORIGIN      
        1 catcgcgatg gacatcgaga ccatcgtccg cgacctcggc cacaacgtca ccggggtcgc
       61 cgtgacccgc gacgaagcgg tcgcccaggc gcggtccaat ccgccggggc tggtgctcgc
      121 cgacatccag ctcgccgatg acagctcggg catcgatgcg gtcaaggaca tattgcacga
      181 gttcaaggtg ccggtgatct tcatcaccgc gttccccgag cggctgctga ctggaactcg
      241 gcccgagccg accttcctga tcaccaagcc gttccagcgc tcgacggtca aggcggcgat
      301 cgcccaggcg ctgttcttcg acgccgcgac ggttccggca gcctagcacc cgacccccgc
      361 gggccgggcg cggcctgcga atttcagccg acggaacatg gccttgcggg gcgcgttggc
      421 ctccgcaagt tccattcgtg acgagagcat aatggccgaa gacgaccaac agcatgtgac
      481 gaccaccgag gcgcgggccg gagcgacccc ccacatgacc cgctatgtgc tggcgattgg
      541 caccggcctg gtgatcgtgc tgttcgcgat catcttgttc gtcgccggtt gagccataca
      601 ttctggcgca atgtccgatg gtgctagggt ctcaaaagag ccgctccgcg gcgcgcttca
      661 aaccggtggg ggcttagtgg ccgacgagga atctcaggac gacgaagcgg tcttggatgc
      721 gcgggccaac cccgtcccgc tgtccgatcc cgagttcaag gaccagctcg gcgcggtaat
      781 tccccacttg cgcgcgttcg ggcgctcgct gtccggaagc cgcgacctcg ccgacgacct
      841 cgtccaggaa acgctgctga aggcgtgggc ggcgcgcaag cgcttccagg ccggcaccaa
      901 catgcgcgcc tggaccttca tcatcctcag gaacctgttc ctgagccaga tgcgccgggc
      961 ccggttcaag ggcgagtggg acgagatcac cgcggcccgg atcctggccg ccccggcgag
     1021 ccaggaccgc cacatcgagc tgggcgacat gcagcgcgcc ctgatgcacc tgccgcagcc
     1081 gcagcgcgag gcgctgatcc tggtcggagc cggcggcttt gcctatgagg aagcggcgga
     1141 aatctgcggt tgcgcggtcg ggacgatcaa gagccgggtg gcccgcggcc gggtcgccct
     1201 ggaaacgctg ctgtcgagcg gcaagatgcc gtcgcggcgc cagcaccgct cggaccccaa
     1261 caaatccgcg ctgcaaatga tcatgggcca ggtcgacgag ctcagccgcg accgctaggc
     1321 cgtcagttga gctggcgcag gagctcggcg aaatcgcggt cgcacggctc caccgccgcc
     1381 gcttcgaagg ctcggcggag cgcggcggta atgccggcgt gagcgggcgg aatgtcgact
     1441 cggatgatcc tccgatccat gttcattgcc gccgccgtca agtgttcacc ttctgacttc
     1501 acatgggggt aacgacgccg cggcgtggaa gtttcgtccg ttacagggcg ggaacggggc
     1561 aaaaggggcg atgaccaggg cgatcggcag cgacagtcgg aacggcgcga tggagcgcgc
     1621 gcgccgcttt tcgccgttcc tgcgcgaggc gctcgaaggg cggccggagc tcggcgattg
     1681 cttcctcgag cagggcagcc gagcggcggt cgcggcggcg ctggcggaag gggacggccc
     1741 ggtcgaggtc gagctccgcc gagcgcggct cgggctggcg ctgacgatcg cgctcggcga
     1801 cctcgcgggc gagctcgggc tcgaggaagt gacgcggacg ctgtccgatt tcgccgacca
     1861 ggcgatcggc cgggcgctcg cggccgcgat cggggagcgg gtcgcggatg ccgggccgac
     1921 cgggttcgcg gtgatcgcgc tcggcaagct gggcagccgc gagctcaatt attcgtcgga
     1981 cgtcgacctg ctgctgctgt tcgatccgat cgcgctgccg aggcgcgagc gcgacgatgc
     2041 cggtgaggcg gcggtgcggg tcgcgcggcg gatgatcgag atcctgcaga agcggaccgc
     2101 cgacggctat gtcgcgcggg tcgacctcag gctccggccg tcgcccgagg tcacccc
//
LOCUS       DASTZO010000053        10618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_605563,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000053 DASTZO010000000
VERSION     DASTZO010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 10618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10618
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605563"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1765
                     /gene="pheT"
                     /locus_tag="VFU20_01415"
     CDS             <1..1765
                     /gene="pheT"
                     /locus_tag="VFU20_01415"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008069757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU5285151.1"
                     /translation="GSFESPVPIRIEDPEGCPAFYGRAIRGLRNGSSPEWIRRRLEAA
                     GQRPISALVDITNYVMIDLGRPSHAYDIARLAGGLVARPARDGETVLALNGKEYPLDR
                     SMTVIADDERVHDIGGIMGGEESGVTAETTDVMLEVAYFDPARIARTGQALGLASDAR
                     SRFERGVDPAFLDDGLAILTGLILDVCGGEPSQVIRAGQPPVADKRVRFDPKRTRTLG
                     GIDVPEERQRAILANLGFGFDGEDSTVPSWRPDIDGPADLVEEIARIHGYDAVPSTPL
                     ERAPGVAHPTATRAQLVERRVRRTAAARGLDEAVTWSFISEAEAAAFGPADWRLVNPI
                     SEEMKVMRPSLLPSLIGAARRNIDRGQSSVRLFEVGRRYLGDSEHPTVALLLAGEKRP
                     RGWRTGKAQGFDAFDAKAEALALLEAAGAPTGNLQLFPDAGPTWHPGKSATLRLGPKS
                     VLAAFGELHPRLVAQLDAPPGTVAAEIYLDAVPQGRPSGRARAAFAPPALQPVTRDFA
                     FLVPADLAAEALVRAVRGADKVAIADARLFDRFETRDGLSLAVEVTFQPTDRSFTDEE
                     IAALSKRIVAAAAKLGAQLRH"
     gene            complement(1754..2446)
                     /locus_tag="VFU20_01420"
     CDS             complement(1754..2446)
                     /locus_tag="VFU20_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU5285152.1"
                     /translation="MHRLRSLLFVAAFYPATLLFIAAGLGAGLFSTPAMLGVVHGWAR
                     FHHWLAEHVLGIRTRVEGAIPPGPYLIAAKHQSMFETIEMVLIARTPVIVLKRELADL
                     PLFGRMTRRYGIIPVDREAGARALREMLAGARRAAATGRPVIIYPEGTRVAPGETPPL
                     RAGFAGLYRMLGLPVVPVAHDSGHLFGRGLDKRPGTVRFLVGDVIPPGLGREEIEARV
                     HAAINALERSVP"
     gene            complement(2446..2982)
                     /locus_tag="VFU20_01425"
     CDS             complement(2446..2982)
                     /locus_tag="VFU20_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEU5285153.1"
                     /translation="MIVRAGSLVLLLYALGFILFTVTLGKPAASDSGRTDAIIVITGG
                     SGRIEHGIDVLEDGRAKRLLVAGADPAVTKRDLIRRLRGNGRLVHCCVDLGSESVDTR
                     SNAEEASRWLARHRYRSFRLVTSDWHMRRARYDFREVLGDQYRIVPDAIRTEPHFMTL
                     FGEYNKYVLRRLAVLLDI"
     gene            complement(2979..3851)
                     /locus_tag="VFU20_01430"
     CDS             complement(2979..3851)
                     /locus_tag="VFU20_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285154.1"
                     /translation="MLDWLFASPAERRLLPGGRFRGPTPFVIAIMTFAMMTVAAAGLA
                     LSNAAGLVAEGIENRYAVQVPPGESMLPRALDAARSARGVLKVQAVPEAEMRKTLERW
                     LGPAGADARLPVPALVTLDVAGDADLAAIAARIEAAAPGARFIAHREKLEPVLRAIRV
                     LQWLALALVALMAAAASAAVVLAARGALDTHRSTIEVMHGIGATDLQVTHLFQRKIAL
                     DSLAGSIVGAIAAGLVLVLLAGGGAAFAGDLMGTTPLGGSDLVVLALLPLALVVLATW
                     VARTAVLAALRTAT"
     gene            complement(3851..4555)
                     /gene="ftsE"
                     /locus_tag="VFU20_01435"
     CDS             complement(3851..4555)
                     /gene="ftsE"
                     /locus_tag="VFU20_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU5285155.1"
                     /translation="MSAIVEFDSVGLRYGTGAEVLRDLDFRLGRGGFYFLTGPSGAGK
                     TSLLKLLYLAQRPTRGRIQLFGEELSDAPRDSLPSFRRRIGVVFQDFRLIRHLSAFDN
                     VALPLRIAGKGEAEVEGPVREMLAWVGLADRASARPATLSGGEQQRVAIARAVISHPE
                     ILVADEPTGNVDAEMAQRLLHLFTALNRLGTTVVVATHDLGLIAATPEAQMVRLENGS
                     MVDPTGALRHPPERRS"
     gene            4681..5580
                     /locus_tag="VFU20_01440"
     CDS             4681..5580
                     /locus_tag="VFU20_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MJ0042-type zinc finger domain-containing
                     protein"
                     /protein_id="HEU5285156.1"
                     /translation="MILTCPACGTQYVVKDGAIPPQGRQVRCASCKHSWHQDPEPAET
                     PAEDEETIAEAALIEPRTGPEAEERAYQEAALAAEDAAAEAPAAEAGEYGEDARSDEQ
                     AEAASYAPVEPVAPPADGLVEAAPAAPVPPAEEPAPAEPVEEAVDDDFRPFRYEPEEA
                     PRRRSPLLTVLLIALAIAALAAAFWFLAPAEWKQRVGIAGAGETPLQLMMTHSDRQKL
                     ASGNELLAVSGRVINPTDEKQDVPPIRAQLRSATGELVYSWTIAPPARTLPPGASATF
                     NSAEVNVPAGGDELTITLGSVKT"
     gene            complement(5584..5820)
                     /locus_tag="VFU20_01445"
     CDS             complement(5584..5820)
                     /locus_tag="VFU20_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285157.1"
                     /translation="MVIFASLALATAALAVPLERVEPQAMAPVRQARATVTILPAARL
                     HFSDIERERPALLRDSVVRAADGTAERLRLVEFE"
     gene            5980..6066
                     /locus_tag="VFU20_01450"
     tRNA            5980..6066
                     /locus_tag="VFU20_01450"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6014..6016,aa:Leu,seq:gag)
     gene            complement(6273..8957)
                     /locus_tag="VFU20_01455"
     CDS             complement(6273..8957)
                     /locus_tag="VFU20_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285158.1"
                     /translation="MRRGRNWLSGLAMLAALGGAGAVGAAASIAAVSAGDGWTAHPDE
                     QFLLDVRIRQHRLGEGVRAYNAPEGPCILLGDFLTTLDVPMTIDLAAKKAAGWAFSEK
                     NRIVIDLPALRASYGAKSEPIAPGTVRETPDGWRVQGPALTRWFGIGVRPMTSGSVLL
                     LESQAKLPVELAAERQQRAAQLKPARFDLAALPQVRLPYRMWRAPALDFVVSGGVTYR
                     AGDGVKVDRRTSVTAAGEIARLSYDAQLSTNDKGIPNNLRLRAYRSDPDGGLLGPLNA
                     THFGLGDVAGLDSRLTGTAASGRGAVVTNRPLFQQTAFDRTRFEGDLPSGWDAEIYRN
                     GELLAFAKPTADQRYLFDDVQLLYGENRISIILYGPQGQVRTREELINVGQDNVPAGK
                     TWYWAGANQPGRDVASLERPPDEVLQPRAQAALSVEHGIDERTSVGALARMMLVDDER
                     LSFLEGTVRRSIGSALVEVGIAGESSGGKAARAQLLAKFGKLNVSAEAVAANDFHLRG
                     QRPVSLREYRLALDAPLRLGRIVVPAHADMHLTDRLDGTKQLEAAARLAANIDRFNLA
                     TDLRYRKQYLRSGPAPPGELNLALIGAGRVGAVRLRGSTSFDIAPGARFRSAELSAYW
                     SASERTDWEGALVYDAAAGRGRARVTHVRRLDSMAVAVTGEAASDGSLALGLNLNFSL
                     DPGGGFKLSRQPLAAAGAVNARVYRDLNDNGIRDSGEPLEKGALITTGRRLSEKATDS
                     NGSVLVGGLATYTPVTVGVDETSLSDPMLVPKKALQVVVPRPGVPANVEIGLVGGGDV
                     EGAIVKSGGLGFEGLDLELVDAAGKTIAIARTDLDGFFLFERVPYGDYRIRLSKDTAE
                     ATGLAFDLGVQVSVSAAKSIVRLGSIHVEPPPRIASLE"
     gene            complement(8988..9815)
                     /locus_tag="VFU20_01460"
     CDS             complement(8988..9815)
                     /locus_tag="VFU20_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HEU5285159.1"
                     /translation="MKFPVPAGPAAALLALTLSTASGPASAGIGDLLVAPTRLVLDGR
                     KGAEVILNNVGDEPATYRISVEFRRMTPDGGLVDVDQPSAEDKLAEEMILYAPRRVTL
                     APHQPQSVRVSARAPQGLPDGEYRVHLLFRAIPPARPVAAQPSEAPKGVRFALTPVYG
                     VTIPVIVRFGNLSAKAGIANVHLETRDGKPAVALDLSRSGDRSTFGEVRVLKPGVKQP
                     IAVQKAVAVYKEVTTRKVSIPVNEEFKGALSGPVTVQYVETYDDGSRTIAETRAVLR"
     gene            complement(10039..10557)
                     /locus_tag="VFU20_01465"
     CDS             complement(10039..10557)
                     /locus_tag="VFU20_01465"
                     /inference="COORDINATES: protein motif:HMM:NF025710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HEU5285160.1"
                     /translation="MMNILRTTAAVAALALTAAPALAAPVGPDRNATATARIVKPLVL
                     TWEQDLDLGTILLSGAGAWTGAVVGVSQAGVLSCANTNVTCSGAVQAARYNVAGTQGQ
                     VVNITAPNVTLVNQNDNTQTLLMTVSSPGTVTLSNSGAPGNDFNLGGSISVSSTTLDG
                     VYLGTFNVTVDY"
BASE COUNT         1570 a         3727 c         3837 g         1484 t
ORIGIN      
        1 gggaagcttc gaaagcccgg tcccgatccg gatcgaggac ccggaaggct gcccggcttt
       61 ctacggccgg gcgatccgcg gattgcgcaa cggctcgtcg cccgagtgga ttcggcgccg
      121 gctcgaggcc gcggggcagc ggccgatctc ggcgctggtc gacatcacca attatgtgat
      181 gatcgacctc ggccggccga gccacgccta cgacatcgcc aggctcgccg gcggcctggt
      241 cgcccgcccc gcccgcgacg gcgagaccgt gctcgccctg aacggcaagg agtatccgct
      301 cgaccggtcg atgaccgtga tcgccgacga cgagcgagtc cacgacatcg gcgggatcat
      361 gggcggcgag gaatcggggg tcaccgccga aaccaccgac gtgatgctcg aggtcgctta
      421 tttcgatccc gcccggatcg cccgcaccgg ccaggcgctc ggcctcgcca gcgacgcgcg
      481 cagccggttc gagcgcggcg tcgacccggc cttcctcgac gacgggctgg cgatcctgac
      541 cgggttgatc ctcgacgtct gcggcggcga gccgtcgcag gtgatccgcg cgggccagcc
      601 gccggtcgcg gacaagcggg tgcggttcga tccgaagcgg acacggacgc tcggcggaat
      661 cgacgtgccc gaggagcggc agcgcgcgat cctcgccaac ctcgggttcg gcttcgacgg
      721 cgaggattcg acggtgccga gctggcggcc ggatatcgac ggccccgccg acctggtcga
      781 ggagatcgcc cggatccacg gttatgatgc cgttccgtcg accccgctgg agcgcgcgcc
      841 gggggttgcc cacccgaccg cgacccgcgc ccagctggtc gagcggcggg tgcgccggac
      901 cgcggccgct cgcgggctcg acgaagcggt cacctggagc ttcatctcgg aagcggaagc
      961 ggccgcgttc ggcccggccg actggcggct ggtcaatccg atcagcgagg agatgaaggt
     1021 gatgcggccc tcgctcctgc cgagcctgat cggcgccgcg cgccgcaaca tcgaccgcgg
     1081 ccagtcgtcg gtgcggctgt tcgaggtcgg ccgccgctac ctgggcgact ccgagcaccc
     1141 gaccgtcgct ttgctgctcg ccggcgagaa gcggccgcgt ggctggcgga ccggcaaggc
     1201 gcagggcttc gacgcgttcg acgccaaggc ggaggcgctg gcgctgctcg aggcggcggg
     1261 cgccccgacc ggcaatttgc agctgttccc cgacgccggg ccgacctggc acccgggcaa
     1321 gtcggcgacc ctgcggctcg ggcccaagtc ggtgctggcg gcgttcggcg agctgcaccc
     1381 gcggctggtc gcccagctcg acgctccgcc cgggacggtg gcggcggaaa tctacctcga
     1441 tgcggtgccg caggggcgcc cgagcggccg agcgcgtgcc gccttcgctc cgccggcgct
     1501 gcagcccgtc acccgcgact tcgccttcct tgttccggcc gaccttgccg ccgaggcgct
     1561 ggtccgagcg gtccgcggcg ccgacaaggt cgcgatcgcc gatgcgcggc tgttcgaccg
     1621 cttcgagacc cgcgacggcc tgtcgctggc ggtcgaggtg acgttccagc cgacggaccg
     1681 gagcttcacc gacgaggaga tcgccgcact gtccaagcga atcgtcgcgg cggccgcaaa
     1741 gctcggagcg cagctaaggc actgagcgct cgagcgcgtt gatcgccgcg tggacgcggg
     1801 cctcgatctc ctcgcgcccg aggcccggcg ggatcacgtc gccgaccagg aagcggacgg
     1861 ttccggggcg cttgtcgagg ccgcgcccga acagatgccc gctgtcgtgc gcgaccggca
     1921 ccaccggcag tccgagcatc cggtacaggc ccgcgaaccc ggcgcgcagg ggcggggtct
     1981 cgcccggcgc cacgcgcgtc ccttcgggat agatgatcac cggccgcccg gtcgcggccg
     2041 ccctcctggc cccggccagc atctcgcgaa gcgcccgggc gccggcctcg cggtcgaccg
     2101 ggataattcc gtaccgccgg gtcatgcgcc cgaacaacgg caggtcggcg agctcgcgct
     2161 tgaggacgat caccggggtt cgggcgatca gcaccatctc gatggtctcg aacatcgact
     2221 ggtgcttggc cgcgatcaga taggggcccg gcgggatcgc gccctccacc cgggtgcgga
     2281 ttccgagcac atgctcggcg agccagtggt ggaagcgcgc ccagccgtgg accactccga
     2341 gcatcgccgg ggtgctgaac aggcccgctc cgagcccggc ggcgatgaac agcagggtcg
     2401 ccgggtagaa tgcggcgacg aacagcagcg agcgcaggcg atgcatcaga tgtcgagcag
     2461 caccgcgagc cggcggagca catatttgtt gtactcgccg aacagggtca tgaagtgcgg
     2521 ctcggtgcgg atcgcgtcgg gcacgatccg gtattggtcg ccgagcacct cgcggaaatc
     2581 gtagcgggcg cggcgcatgt gccagtcgct ggtgaccagc cggaacgagc ggtagcggtg
     2641 cctggcgagc cagcgcgagg cttcctcggc gttggagcgg gtgtcgactg attcgctgcc
     2701 gaggtcgacg cagcaatgga ccagccggcc gttgccgcgc agacgccgga tcagatccct
     2761 cttggtcacc gccgggtcgg cgccggcgac cagcagccgc ttcgcccggc cgtcctcgag
     2821 gacgtcgatg ccgtgctcga tccggccgct tccgccggtg atgacgatga tcgcgtcggt
     2881 gcgcccggaa tcgctcgccg cgggcttgcc gagggtcacg gtgaacagga tgaagccgag
     2941 cgcgtagagc agcaggacca gcgagccggc gcggacgatc atgtcgccgt ccggagggcc
     3001 gccaggaccg cggtccgggc gacccaggtc gccagcacca ccagcgccag cggcaggagc
     3061 gcgaggacga cgaggtcgct gccgccaaga ggggtcgtgc ccatcaggtc gccggcgaac
     3121 gccgctccgc cgccggcgag cagcaccagc accagcccgg cggcgatcgc cccgacgatg
     3181 ctcccggcga gcgaatcgag cgcgatcttg cgctggaaca gatgggtgac ctggaggtcg
     3241 gtggcgccga tgccgtgcat cacctcgatg gtcgagcggt gggtgtcgag cgccccgcgg
     3301 gcggcgagca ccaccgccgc cgaggccgcc gccgccatca gcgcgaccag cgccagcgcc
     3361 agccattgca gcacccggat cgcgcgcagc accggctcga gcttctcgcg gtgggcgatg
     3421 aagcgcgcgc ccggggccgc ggcctcgatc cgggcggcga tggcggcgag gtccgcgtcg
     3481 ccggcgacgt cgagcgtgac cagcgccggc accggcagcc gggcgtcggc cccggccggc
     3541 cccagccagc gttcgagggt cttgcgcatc tcggcttcgg gaacggcctg caccttcagc
     3601 accccgcgcg ccgagcgggc cgcatcgagc gcccgcggaa gcatgctctc tcccggcggg
     3661 acctgcaccg cgtagcggtt ctcgatgccc tcggcgacca gcccggcggc gttggacaag
     3721 gccagcccgg ccgcggcgac ggtcatcatc gcgaaggtca tgatcgcgat cacgaacgga
     3781 gtcggcccgc gaaaccggcc cccagggagc agccggcgct cggccggcga ggcgaacagc
     3841 cagtcgagca ttaagagcgc ctctcgggag gatgccggag cgcgccggtc gggtcgacca
     3901 tcgagccgtt ctcgagccgc accatctgcg cctccggggt cgcggcgatc agcccgagat
     3961 cgtgggtcgc caccaccacc gtcgtcccga gccggttcaa ggcggtgaac aggtgcagca
     4021 ggcgctgcgc catctcggca tcgacattgc cggtcggctc gtcggcgacg aggatttcgg
     4081 ggtggctgat caccgcccgg gcgatggcga cgcgttgctg ctcgccgccc gacagggtcg
     4141 cgggcctggc gctcgcccgg tcggcgagcc cgacccaggc gagcatctcg cggaccggcc
     4201 cctcgacctc ggcctcgccc ttgccggcga tgcgcagcgg cagagcgaca ttgtcgaacg
     4261 ccgacagatg gcggatcagc cggaagtcct ggaacaccac cccgatccgg cgccggaagc
     4321 tcggcaggga atcgcgcggc gcgtccgaca attcttcgcc gaacagctgg atccgcccgc
     4381 gggtcgggcg ctgggcgagg tagagcagct tcaatagcga cgtcttgccg gcgcccgacg
     4441 ggccggtgag gaaataaaag ccgccgcggc cgagccggaa atcgaggtcg cgcagcactt
     4501 cggcgccggt gccgtagcgc agccctacgc tatcgaattc gacgatcgcc gacagcgccc
     4561 gatcccctcc cgctcagtgg cccgctcacg ccgcgcttgt cgcacagccc tcgccgcctt
     4621 gccaatgccc ccgcattcct gtttatcgag cgttggtcga cccgataagg gctcttgctg
     4681 atgatcctca cctgcccggc gtgcggcacc caatatgtgg tgaaggacgg ggcgatcccg
     4741 ccgcaggggc gccaggtgcg ctgcgcgtcg tgcaagcaca gctggcacca ggaccccgag
     4801 cccgccgaga ctccggccga ggacgaggag acgatcgccg aggcggcgct gatcgagccg
     4861 cgcaccggcc ccgaggccga agagcgcgcc taccaggaag cggcgctcgc cgccgaggat
     4921 gcggcggcgg aggccccggc cgcagaggct ggcgaatatg gcgaggacgc ccgctccgac
     4981 gagcaggccg aggccgccag ctacgcgccc gtcgagccgg tcgcgccgcc cgccgacggg
     5041 ctggtcgaag cggctcccgc agcgccggtc ccgccggccg aggagccggc cccggcggaa
     5101 cccgtcgaag aggcggtcga cgacgatttc cgcccgttcc gctacgagcc cgaggaggcg
     5161 ccgcggcggc gcagcccgct gctgacggtg ctgctcatcg cgctggccat cgccgcactg
     5221 gccgcggcct tctggttcct ggcgccggcc gagtggaagc agcgggtcgg aatcgccggc
     5281 gccggcgaaa ccccgctgca gctgatgatg acccacagcg accgccagaa gctggccagc
     5341 ggcaacgagc tgctcgcggt cagcggccgg gtgatcaacc cgaccgacga gaagcaggac
     5401 gtgccgccga tccgcgccca gttgcgcagc gcgaccggcg aactggtcta cagctggacc
     5461 atcgctccgc cggcgcggac tcttccgccg ggcgccagcg cgaccttcaa cagcgccgag
     5521 gtcaacgtgc cggcgggcgg cgacgagctg accatcaccc tgggctcggt aaagacctga
     5581 gccttattcg aactcgacca gcctgagcct ctccgccgtc ccgtcggcgg ctcggacgac
     5641 gctgtcgcgc aacaaagccg gccgctcgcg ctcgatgtcc gaaaaatgca gccgcgcggc
     5701 gggaaggatg gtcaccgtcg cccgcgcctg gcgcaccggc gccatggctt gcggctcgac
     5761 ccgctctagg gggacggcga gcgcggccgt tgccagtgcc agcgaggcga aaatgaccat
     5821 cccggacgcg taacgcccga cggcccaaat ggtaaaggcg gtcttaacct cctgtcccgc
     5881 agggggacga atcgggcggc attgcctgcc ctggagccct ttgctagacg ccgcgcctcg
     5941 ccagtcggcg gctggtcgcc gccaagctct tcatgacgtg cggtcgtggc ggaactggta
     6001 gacgcgcagc gttgaggtcg ctgtggccga aaggccgtgg aagttcgagt cttctcgacc
     6061 gcaccatccc ttcaggctcc cggcggcgat ccctttcgca cttgcgacgc attagcgaca
     6121 atatccttgg cagttcgaaa ggccccgggc ggcgcatggg ctgggcaggc gcgtgcccta
     6181 gacgcgggga ggcgcggcgc gcttccgacg ggaaggcgcg gccattcggt ggaactaggg
     6241 catgaagtcg tgcgagacga ctgtggcggg ggttattcga gcgaggcgat gcgcggcggg
     6301 ggctcgacgt ggatcgagcc gaggcggacg atcgatttcg ccgcgctgac gctgacctgg
     6361 acgccgaggt cgaacgccag gccggtcgct tcggcggtgt ccttcgacag gcggatccga
     6421 tagtcgccat agggcacgcg ctcgaacagg aagaagccgt cgaggtcggt gcgggcgatg
     6481 gcgatcgtct tgccggcggc atcgaccagc tcgaggtcga gcccttcaaa gccgagcccg
     6541 ccgctcttga cgatcgcgcc ctcgacgtcg ccgccgccga ccaggccgat ctcgacattg
     6601 gccggaactc cgggcctcgg aaccaccacc tgcagcgcct ttttcggcac cagcatcggg
     6661 tcggacaggc tggtctcgtc gaccccgacg gtgaccgggg tgtaggtcgc aagcccgccg
     6721 accagcaccg agccgttgga atccgtggct ttttcggaca gccggcggcc ggtggtgatc
     6781 agcgcccctt tctcgagcgg ctctccggaa tcgcggatcc cattgtcgtt gagatcgcgg
     6841 tagacccgcg cgttgaccgc gcccgcggcg gcgagcggct ggcgcgacag cttgaacccg
     6901 ccgcccgggt ccaatgagaa gttgaggttg aggccgagcg ccagcgagcc gtcgctggcc
     6961 gcctcgccgg tcaccgccac cgccatcgaa tcgagccgcc ggacgtgcgt gacgcgcgcc
     7021 cggccgcgcc cggcggcggc atcgtagacc agcgccccct cccagtcggt ccgttccgac
     7081 gccgaccaat aggccgacag ctccgccgag cggaaccggg ccccgggggc gatgtcgaag
     7141 ctggtcgagc cgcgcagccg gacggcgccg acccggccgg cgccgatcag cgccaggttg
     7201 agctcgcccg gcggcgccgg ccccgagcgc aaatattgct tgcggtagcg aaggtcggtg
     7261 gcgaggttga agcggtcgat gttggccgcc agccgggccg ccgcttcgag ctgcttggtg
     7321 ccgtcgagcc ggtcggtcag gtgcatgtcg gcgtgcgcgg ggacgacgat ccgcccgagc
     7381 cgcagcggcg cgtcgagcgc cagccggtat tcgcgcaggc tgaccggccg ctggccacgc
     7441 aaatgaaaat cattcgcagc aacagcctcg gcgctgacgt tgagcttgcc gaacttggcc
     7501 aagagctggg cccgggccgc cttgccgccc gagctttcgc cggcaatccc gacctcgacc
     7561 agcgccgagc cgatcgagcg ccggaccgtg ccctcgagaa agctcagccg ctcgtcgtcg
     7621 accagcatca tccgtgccag cgcgccgacc gaggtgcgct cgtcgatccc gtgctcgacc
     7681 gacagcgcgg cctgcgccct gggctgaagc acttcgtcgg gcgggcgctc gagcgaggcg
     7741 acgtcgcgcc ccggctggtt ggcgccggcc cagtaccagg tcttgccggc cgggacattg
     7801 tcctggccga cgttgatcag ctcctcgcgg gtccggacct ggccctgcgg cccgtagagg
     7861 atgatggaaa tccggttctc gccgtaaaga agctgaacgt cgtcaaacag ataacgctgg
     7921 tccgcagtcg gcttggcgaa ggccagcagc tcgccgttgc ggtagatctc ggcgtcccag
     7981 ccgctcggca aatcgccttc gaagcgggtg cggtcgaacg cggtctgctg gaacagcggc
     8041 cggttggtga cgaccgcgcc gcggccgctg gccgccgtgc cggtcagccg gctgtcgagc
     8101 ccggcgacgt cgcccaggcc gaaatgggtc gcgttcagcg gccccagcag cccgccgtcg
     8161 gggtcggaac ggtaggcgcg cagccgcaaa ttgttgggga tgcccttgtc gttggtcgac
     8221 agctgggcgt cgtaggacag gcgggcgatc tctccggcgg cggtgaccga ggtgcggcgg
     8281 tcgaccttga cgccgtcgcc ggcgcggtag gtgacgccgc cgctgacgac gaagtcgagc
     8341 gccggggcgc gccacatccg gtacggcagg cgcacctgcg gcagggccgc gaggtcgaac
     8401 cgggccggct tgagctgggc ggcgcgctgc tggcgctcgg ccgcgagctc gaccggcagc
     8461 ttggcctgcg attcgagcag cagcaccgat cccgaggtca tcggccggac tccgatcccg
     8521 aaccagcggg tcagtgccgg cccctggacg cgccagccgt cgggggtctc gcgcacggtg
     8581 ccgggggcga tcggctcgct tttggctccg tagctcgccc ggagcgcggg caagtcgatg
     8641 acgatccggt ttttctcgct gaacgcccag cccgcggctt ttttggcggc caggtcgatc
     8701 gtcatcggca cgtcgagcgt ggtcaggaag tcgcccagga ggatgcacgg gccctcgggc
     8761 gcattgtagg cgcgcacccc ctcgccgagc cgatgctggc ggatccggac gtcgagcagg
     8821 aattgctcgt cggggtgcgc ggtccagccg tcgccggccg aaaccgccgc gatcgacgcc
     8881 gcggcgccga ccgcgccggc gccgcccagc gccgccagca tcgccagccc gctcagccag
     8941 ttgcggccgc ggcgcatggg cttggggtcc cttgcctccg tcccggacta gcgcagcacc
     9001 gcgcgggttt cggcgatggt ccgcgagccg tcgtcgtagg tctcgacgta ctggacggtg
     9061 accgggccgc taagggcgcc cttgaattcc tcgttgaccg ggatcgacac cttgcgggtc
     9121 gtgacttcct tgtagaccgc gaccgccttc tggacggcga tcggctgctt gaccccgggc
     9181 ttgagcaccc ggacctcgcc gaaggtcgag cggtcgcccg agcggctgag gtcgagcgcc
     9241 acggccggct tgccgtccct ggtctcgaga tggacgttgg cgatccccgc cttggccgac
     9301 aggttgccga agcgcacgat caccgggatg gtcaccccgt agaccggggt caaagcgaag
     9361 cgcacgccct tgggggcctc ggagggctgg gcagccaccg gcctggccgg cgggatcgcc
     9421 cggaacagca gatggacgcg gtattcgccg tcgggaaggc cctgcggcgc gcgggccgac
     9481 acccgcaccg attgcggctg gtgcggcgcc agggtcaccc ggcgcggagc gtagaggatc
     9541 atttcctcgg cgagcttgtc ctcggcgctc ggctggtcga cgtcgaccag gccgccgtcg
     9601 ggggtcatgc gccggaactc gaccgagatc cggtaggtcg cgggctcgtc gccgacattg
     9661 ttgaggatca cctcggcgcc cttgcggccg tcgagcacca gccgggtcgg tgcgaccagc
     9721 aggtcgccga tgcccgccga cgcgggcccg gacgcggtgg aaagcgtgag tgcgagcagc
     9781 gccgcggccg gcccggcagg aactggaaac ttcatcgtgg cgatccccga atttgctcca
     9841 ttgtggagcg gtcgccccgt cttgcctccg taatggttga ttttcggtca accatccggg
     9901 ttcaacggca attcttcacg acgggacggt ggtggccgag cgggggagcg ggggcgtccg
     9961 cgcaaggaaa aggggccgcc gttgccggcg acccctccca ctggccgttc cctggcggga
    10021 accgcgaact tcaaacgatc aatagtcgac ggtcacattg aacgtgccga gatagactcc
    10081 gtcgagcgtc gtcgagctca ccgagatcga gccgccgagg ttgaagtcgt tgcccggagc
    10141 gcccgagttg gagagagtca ccgtgcccgg gctgctgacg gtcatcagca gcgtctgggt
    10201 gttgtcgttc tggttgacca gagtgacgtt cggcgcggtg atgttgacca cctggccttg
    10261 cgtgccggcg acgttgtagc gcgccgcctg cacggcgccc gagcaggtga cgttggtgtt
    10321 ggcgcagctc agcactccgg cctgcgaaac gccgaccacc gcgccggtcc aggcgccggc
    10381 gcccgacaac aggatggtgc cgaggtcgag gtcctgctcc caggtcagga cgagcggctt
    10441 gacgatccgc gcggtggcgg tcgcgttgcg gtccggtccg accggcgcag cgagcgccgg
    10501 agcggcggtc agagccagag cggcaaccgc cgccgtggta cgaagaatgt tcatcatggc
    10561 ttgtatcccc actggttccc aaatctgtca gcggctgccc gaccgctggt tgggtcac
//
LOCUS       DASTZO010000054         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1231997,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000054 DASTZO010000000
VERSION     DASTZO010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231997"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1810)
                     /locus_tag="VFU20_01470"
     CDS             complement(<1..1810)
                     /locus_tag="VFU20_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5285161.1"
                     /translation="MSAIAALLLSTSVSAVQPEETAAPPSPPAAQQGPQAPPPHSVHE
                     GHEGEIVVIGRYVRELDLLAGKSVLEGEQLQRDVRTQIGESLTKLPGVSATSFSPGAS
                     RPVLRGFQGERIRVLTDGIGSIDVSNTSADHAVTIDPLTAERIEVLRGPAVLLFGGQA
                     IGGAVNVIDRRIPHVVPEEGYHIDAIGGLGSAADERSAGAAVDVALGASGLVLHVDGT
                     LRKTDDLRAGGYVLSPQLRAEQFAIAEEELEEGHVEESEEALELAGLRGEIPNSATEQ
                     KSGGIGLALIRDRFSIGGSISLFDSKYGVPSRPGAEHHHGEEGEEGEEESEEEEHGDA
                     PVSIDLRQWRGDLRAEYELEQGFLDRVRLRIGAADYEHTEFEGDEVGTVFKSEGLEGR
                     LEFVQRDRDGWRGASGAQYFRRDFSAVGAEAFLPPNKTSQLGLFTLQEFAIGKVGVEA
                     SARYEHSGVNSDDLGIERNFNALSAALGASYELASQVKTGFNLSRTERAPSAEELASN
                     GPHIATQAFEVGDPDLKKERSLGAEAYFRVDRSNVELSLTAFANWFDDFIFEAATGEE
                     QDELPVFQYFQRDATYYGFEAEASATLFRAGQFKFVG"
     gene            complement(2011..2241)
                     /locus_tag="VFU20_01475"
     CDS             complement(2011..2241)
                     /locus_tag="VFU20_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285162.1"
                     /translation="MDPLSIGLLAAGIFAALGMASMAALKGWQGWLELKRFELTHGNA
                     DKALPPATGRIELADLRERVRKLEAIAAGIDL"
     gene            complement(2241..2636)
                     /locus_tag="VFU20_01480"
     CDS             complement(2241..2636)
                     /locus_tag="VFU20_01480"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU5285163.1"
                     /translation="MNNRYSLNRRDKKLAGVCSTIGDIFNIDPTFIRIGFVAAVFLIS
                     WEVALVTYAALGIYFSIERKREVDAGVLGRRRSEFERMGDAPKVRPSVHAMRTELDET
                     DRRLMAIDDHLATPNHELAREIDALREEK"
     gene            complement(2656..>3106)
                     /locus_tag="VFU20_01485"
     CDS             complement(2656..>3106)
                     /locus_tag="VFU20_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEU5285164.1"
                     /translation="EKAGLALEKGREDLARAALAEKQKAAEMAEGLHAEISQIEQVLR
                     GYEADIARLQAKLAEARARQNAIASRFESAMTRARTSELLHGSRTEEAFAKFELLERR
                     ADFAEGRADALALAGPKSLEEEIAELRSADNVEAELEAMKAALSARN"
BASE COUNT          474 a         1059 c         1031 g          542 t
ORIGIN      
        1 cgccgacgaa cttgaactgt cccgcgcgga atagggtcgc cgaagcttca gcctcgaacc
       61 cgtaataggt cgcgtcccgc tggaaatatt ggaagacggg cagctcgtcc tgctcctctc
      121 cggtcgcggc ttcgaagatg aaatcgtcga accaattggc gaacgcggtc aggctgagct
      181 cgacattgga ccggtcgacc cggaaatagg cctcggcgcc aaggctcctt tccttcttca
      241 gatcagggtc gccgacctcg aaggcctggg tggcgatgtg cggcccgttg gaggccagct
      301 cctcggccga aggcgcgcgc tcggtccgcg acaggttgaa gcccgtcttg acctgggagg
      361 cgagctcgta agacgcgccc agcgccgccg acaaagcgtt gaagttgcgc tcgattccga
      421 gatcgtcgga attgacgccg ctgtgctcgt atcgagccga agcctcgact ccgaccttgc
      481 cgatcgcgaa ttcctggagc gtgaacaggc cgagctggct ggtcttgttc ggcggcagga
      541 aggcctcggc gccgaccgcg ctaaagtcgc gccggaaata ttgcgcgccg ctggcgccgc
      601 gccagccgtc gcggtcgcgc tggacgaact cgagccggcc ttcgagccct tccgacttga
      661 agacggtccc gacctcgtcg ccctcgaact cggtgtgctc gtagtcggcc gcgccgatcc
      721 ggagccggac gcggtcgagg aagccctgct cgagttcgta ttcggcccgc aggtctcccc
      781 gccattggcg caggtcgatc gagaccggcg cgtcgccatg ttcttcctcc tcgctctcct
      841 cttcgccctc ctcgccctcc tcgccatggt ggtgctcggc gccgggacgc gacgggacgc
      901 cgtacttgct gtcgaacagg ctgatcgatc cgccgatgct gaagcggtcg cgaatcagcg
      961 ccaggccgat cccgccgctc ttctgctcgg tcgcgctgtt ggggatctcg ccgcgcaagc
     1021 cggcgagctc gagggcttcc tcggactctt cgacatggcc ctcctcgagc tcttcctcgg
     1081 cgatcgcgaa ctgctcggcg cggagctgcg gcgacagcac gtagccgccg gcgcgaaggt
     1141 cgtcggtctt ccgcagcgtg ccgtcgacat ggagcaccag gccgcttgcg ccgagggcga
     1201 catcgaccgc cgcgccggcc gatcgttcgt ccgcggccga gccgaggccg ccgatcgcgt
     1261 cgatatgata gccctcctcc ggcaccacgt gcggaatgcg ccggtcgatg acattgaccg
     1321 cgccgccgat cgcctgcccg ccgaacagca gcaccgcggg gccgcgtaga acctcgattc
     1381 gctcggccgt cagcgggtcg atcgtcaccg catggtcggc ggaggtgttc gacacgtcga
     1441 tcgagccgat cccgtcggtg agcacccgga tccgctcgcc ctggaagccc cgcagcaccg
     1501 gccgcgacgc accgggcgaa aaggaggtgg cggacacgcc cggcagcttg gtcagcgatt
     1561 cgccgatctg ggtccgcacg tcccgctgga gctgctctcc ctcgagcacc gatttgccgg
     1621 cgagaaggtc gagctcgcgg acatagcggc cgatgacgac gatctcgccc tcgtggccct
     1681 cgtggaccga atgcggtggg ggcgcctgcg gaccctgctg ggcggctggc ggggagggcg
     1741 gcgcagctgt ctcttccggc tgcacggccg aaacgctggt cgacagaaga agggcggcga
     1801 tcgcgctcaa gtgtatctcc caaggggtgg ctcgcggccc gttacgcgat acattataac
     1861 agcacaatca cggaagcggg tttgggcggg cgaaccgtcg cccgggcggt gcagcctgtc
     1921 gaaaaacgaa ggcgcagccc gggaggggga aatcccgggc cgcgcggccg cttcgcctgg
     1981 ggagcgcggg agcggcggtg gcttggtggc ttaaaggtcg atgccggcgg cgatcgcctc
     2041 cagtttgcgg actcgctcgc gaaggtcggc tagctcgatt cgcccggtcg ccggcggcag
     2101 cgccttgtcg gcattgccgt gggtcaactc gaagcgcttc agctcgagcc agccctgcca
     2161 gcccttgagc gcggccatgc tcgccatccc aagcgccgcg aaaatgcccg cggccagcag
     2221 cccgatagac agagggtcca tcacttctcc tcccgcagcg cgtcgatctc gcgcgccagc
     2281 tcatggttcg gggttgcaag gtgatcgtcg atcgccatca gccggcggtc ggtctcgtcg
     2341 agctcggtgc gcatcgcgtg aaccgacggg cgaaccttcg gagcgtcgcc catgcgctcg
     2401 aactcgctgc gccgccggcc caggacgccg gcgtcgacct cgcgcttgcg ctcgatcgag
     2461 aaatagatgc cgagcgcggc gtaggtgacc agcgcgactt cccagctgat caggaacacc
     2521 gcggcgacga agccgatgcg gatgaaggtc gggtcgatgt tgaagatgtc gccgatggtc
     2581 gagcagacgc cggccagttt cttgtcgcga cggttaagcg aatagcggtt gttcatttcc
     2641 ccgttttcct tctcttcaat tccgggccga cagcgcggcc ttcatcgctt ccagctcggc
     2701 ctcgacattg tcggcggacc gaagctcggc aatctcttcc tcaaggcttt tcggaccggc
     2761 cagggccagc gcatcggcgc gtccttcggc gaagtcggcg cggcgctcga gcaactcgaa
     2821 cttggcgaac gcttcttcgg tccggctgcc gtgaagcaac tcgctggtgc gggcgcgggt
     2881 catcgcgctc tcgaaccgcg aagcgatcgc attctggcgg gcccgggctt cggccagctt
     2941 ggcctgcagg cgggcgatat ccgcctcata gccgcgcagg acctgctcga tctggctgat
     3001 ctctgcatga agtccctcgg ccatttcagc tgccttctgc ttttcggcga gcgcagcgcg
     3061 ggccaggtcc tcacgaccct tttcaagcgc cagccctgcc ttttcc
//
LOCUS       DASTZO010000055         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_813257,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000055 DASTZO010000000
VERSION     DASTZO010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813257"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..819
                     /locus_tag="VFU20_01490"
     CDS             <1..819
                     /locus_tag="VFU20_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285165.1"
                     /translation="CIENNGRRIGFGNANDMLTFYRKTYKRAAKDATQLNPFEIGIVY
                     PAEHPARNGHDQTDGQRPAALPAQPPGPMASATESPGEAGATAEKAEKAPATDDEKAR
                     RRAESVERAALMGRLPPEVKMRVAQREITLVEALTGAGLEIPEWLRTGASAPAATPQP
                     PGDVEPSATGADPESERKGELDAQKAEAAVEERNLIGGAPGDDTQPAAPRTAADAAPP
                     SVRADPKEAKRAEAMRRAALMAKLPGEVKLRVASREITLDDALREAGIDPEALA"
     gene            980..1603
                     /locus_tag="VFU20_01495"
     CDS             980..1603
                     /locus_tag="VFU20_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HEU5285166.1"
                     /translation="MSTKIALDGVGGQGVRVIAGVMGALLARMGKEVTVLFVYDSSVR
                     GSMSDAFLIFDDEPIANPVVEEADIMLKLADKGHLRIQGRTVITDLGLGLPGQEQIPF
                     ASLGSQHFGKELFGNMIALGRLLRLAGIDFDEASIRESLPRRFVDENVAAIKFGYQLT
                     PESIASYAAAAPPSRFEMKDAATGGLGGHPHGASVGMGSIAEVGESG"
     gene            1790..>2074
                     /locus_tag="VFU20_01500"
     CDS             1790..>2074
                     /locus_tag="VFU20_01500"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285167.1"
                     /translation="MAIAELSQETARRAPKVLLHDHLDGGLRPRTVLELAGEAGYRDL
                     PTTDPDELAAWFTRGADRKSLELYLEGFRHTVAVLQTRDAIERVAAECAED"
BASE COUNT          326 a          731 c          772 g          245 t
ORIGIN      
        1 tgcatcgaga acaacggccg ccgcatcggc ttcggcaacg ccaacgacat gctcaccttc
       61 taccgcaaga cgtacaagcg cgcggccaag gacgcgaccc agctcaaccc gttcgagatc
      121 gggatcgtgt acccggccga gcatccggcg cgtaacggcc acgaccagac cgatgggcag
      181 cgcccggcgg cgctgccggc gcagccgccc ggaccgatgg cgtcggccac cgagtcgccc
      241 ggcgaggccg gcgcgacggc cgagaaggcc gagaaggcgc cggccaccga tgacgagaag
      301 gcgcgtcgcc gggccgagtc ggtcgaacgc gccgcattga tgggcaggct gccgcccgag
      361 gtgaagatgc gcgtcgcgca gcgcgagatc acgctcgtcg aggcgctcac cggcgcgggg
      421 ctcgagatcc ccgaatggct gcgcaccggg gccagcgccc cggccgccac tccacagccg
      481 ccgggtgacg tcgagccgtc ggccaccggg gcggaccccg agtccgagcg caagggcgag
      541 ctggacgccc agaaggcgga ggcggccgtc gaggagcgca acctgatcgg cggggcgccg
      601 ggagacgaca cgcagccggc cgcgccgcgg acggcggccg acgcggcgcc accatcggtc
      661 cgggcggacc cgaaggaagc gaagcgcgcc gaggcgatgc gccgggccgc gctgatggcg
      721 aagctgccgg gcgaggtgaa gctgcgcgtc gccagccgcg agatcaccct cgacgacgcg
      781 ctgcgcgagg ccggcatcga ccccgaggcg ctggcatgaa cgctcaccag gtgaggccta
      841 tcggtgccgc gagggctcag ctctcgcctg ctgggagata cccgcgtgat cagcgccgcg
      901 ccgcccggtt gcgctcacca gctgagagat gcgcccgggg ccggcgcgcc agcaccggat
      961 tgcccccagg tgccgagagt tgagcaccaa gatcgcgctc gacggggtcg gcgggcaggg
     1021 ggtccgggtg atcgccgggg tgatgggcgc gctcctggcc cgcatgggca aggaggtcac
     1081 cgtcctgttc gtctacgact cgagcgtgcg cggctcgatg tccgacgcgt tcctcatctt
     1141 cgacgacgag ccgatcgcca acccggtggt cgaggaggcg gacatcatgc tcaagctggc
     1201 cgacaagggg cacctccgca tccagggcag gacggtcatc accgacctgg ggctcgggct
     1261 gccgggccag gagcagattc cgttcgcgtc gctcggctcg cagcacttcg gcaaggagct
     1321 gttcggcaac atgatcgcgc tggggcggct gctgcgcctg gcaggcatcg acttcgacga
     1381 ggcctcgatc cgcgagtcgc tgccgcgccg cttcgtcgac gagaacgtgg cggccatcaa
     1441 gttcggctac cagctcactc ccgagtcgat cgcgtcgtac gccgcggccg cgccgccgtc
     1501 gcgcttcgag atgaaggatg cggcgaccgg cggcctcggc ggccacccgc atggggcgag
     1561 cgtcgggatg ggcagcatcg ccgaggtggg cgagagcggc tgaccctccg gtgttgcatc
     1621 cggtgtcggc aaggagcgga agaccggccg acgccggcgg gccgggcccg aattcgaccg
     1681 tgttcggtgc gccagacgca atgaatgcgg tcattgcgcc gcgagctcct ccggttgccg
     1741 acacccgatg cacacccccg ctcggcggct cgctacactg cgcggcacga tggccatcgc
     1801 cgagctgagc caggagacgg cgcgacgcgc gccgaaggtg ctgctccacg accacctcga
     1861 cggggggctg cggccgcgga ccgtcctcga gctggcgggc gaggccggct atcgggatct
     1921 cccgacgacc gatccggacg agctcgcggc atggttcacc cgcggcgcgg accgcaagtc
     1981 gctcgagctg tacctggagg gcttccggca cacggtggcg gtgctccaga cgcgggacgc
     2041 catcgagcgc gtcgccgccg agtgcgccga ggac
//
LOCUS       DASTZO010000056         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_161487,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000056 DASTZO010000000
VERSION     DASTZO010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161487"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2438
                     /locus_tag="VFU20_01505"
     CDS             <1..2438
                     /locus_tag="VFU20_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HEU5285168.1"
                     /translation="LDSEQAMTIFLKRIAAEPDIARVPVMIDSSKWSVIEAGLKCVSG
                     KPVVNSISLKEGEGPFLELARKVRDYGAATVVMAFDEVGQADTATRKVAICERAYRLL
                     VADGTEPQDIIFDPNIFAVATGIDEHRRYALDFIEAAREIRARCPGVHISGGLSNLSF
                     SFRGNEPVRRAMHSVFLYHAIPAGLDMAIVNAGQLDVYDLIDPELREACEDVILDRRP
                     KDGGDATERLVELAERYRGTDAAQEKIEAEWRSMPVGERLSHALVKGIDAHIVADTEE
                     ARALFARPIEVIEGPLMDGMNVVGDLFGSGKMFLPQVVKSARVMKKAVAHLLPFIEAE
                     KEATGAAQGKGRVVMATVKGDVHDIGKNIVGVVLQCNGFEIIDLGVMVPWQDILQAAN
                     DNKADIIGLSGLITPSLDEMVTVAGEMQRLGLKTPLLIGGATTSKAHTALRIDPAYDG
                     PVIHVLDASRAVGVASSLVSDTQAEPLVASTAQEYDRLRKSRERSGHSELATLEEARA
                     NAFPFDPDGQPPPPQRPGLHQFGEWPLRDLRASIDWTPFFRAWELAGNYPAILDDPVV
                     GESAKSLFADAQDMLEKLIAERWVTPSATVGLWRCKREGDDVLVLAGNDWTRVPFLRQ
                     QVKKREGRPNMCLADFINPEGEDWIGGFAVGIHGLEPHLERFKAGNDDYSDILLKALA
                     DRLAESLAEVLHAEVRNRIWGYADEHFSNEQLIREKYQGIRPAPGYPACPDHSLKPML
                     FDMLGGTPGGVTLTENYAMLPTSAVSGFYFGHRDAQYFGVARIGRDQLEDYARRRGVG
                     IETAERWLRPNLD"
     gene            2539..>2977
                     /locus_tag="VFU20_01510"
     CDS             2539..>2977
                     /locus_tag="VFU20_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HEU5285169.1"
                     /translation="MLRFFLLLLAFLAMPSLAEARENAIRPQLVAEGPAPPGGEVELA
                     IDMRTRPGWHGYWRNPGDAGLPMEVDWTLPPGFAVGPLRYPVPTRLTIAGLMNYVYER
                     DYAVLVRLKVPPDARGSVPIRADARWLACTDKVCVPESGSFALT"
BASE COUNT          496 a         1002 c         1021 g          458 t
ORIGIN      
        1 tgctcgattc agagcaggcg atgaccatct tcctcaagcg catcgccgcc gagccggaca
       61 tcgcccgcgt tccggtgatg atcgacagct ccaagtggag cgtgatcgag gccggcctca
      121 aatgcgtgtc gggcaagccg gtcgtcaact cgatcagcct caaggaaggc gaggggccgt
      181 tcctggagct cgcccgcaag gtgcgcgact atggcgcggc aacggtggtg atggccttcg
      241 acgaggtcgg gcaggccgac acggcgacac gcaaggtcgc gatctgcgag cgcgcctacc
      301 ggctgctggt cgccgacggg accgagccgc aggatatcat cttcgacccc aacatcttcg
      361 cggtcgcgac gggcatcgac gagcaccgcc gctacgcgct cgacttcatc gaggccgcgc
      421 gcgagatccg cgcgcgctgc cccggagtcc atatctcggg cgggctttcg aacctgagct
      481 tttcgttccg cggcaacgag ccggtgcgcc gggcgatgca cagcgtgttc ctctaccacg
      541 cgatccccgc cggcctggac atggccatcg tcaacgccgg ccaactcgac gtctatgacc
      601 tcatcgaccc ggagctgcgc gaagcgtgcg aggacgtgat cctcgaccgg cggccaaagg
      661 acggcgggga cgctacggaa cggctggtcg agctcgccga gcgctaccgc ggcaccgacg
      721 ccgcgcagga gaagatcgag gccgagtggc gctcgatgcc ggtcggcgag cggctgtcgc
      781 acgctttggt caagggcatc gatgcgcaca tcgtcgccga caccgaggaa gcgagggctt
      841 tgttcgcccg cccgatcgag gtgatcgaag ggccgctgat ggacgggatg aacgtcgtcg
      901 gcgacctgtt cggctcgggc aagatgttcc tgccgcaggt ggtgaagtcg gcgcgggtga
      961 tgaagaaggc ggtcgcgcat ttgcttccgt tcatcgaggc ggagaaggag gcgaccggcg
     1021 ccgcccaggg caagggcaga gtggtgatgg cgaccgtcaa gggcgacgtc cacgacatcg
     1081 gcaagaacat cgtcggcgtc gtcctgcagt gcaacggctt cgagatcatc gacttgggag
     1141 tcatggttcc gtggcaggac atcctccagg cggcgaacga caacaaagcc gacatcatcg
     1201 gcctgtccgg gctgatcacc ccgtcgctcg acgagatggt gacggtcgcg ggcgagatgc
     1261 agcggctggg cctcaagacg ccgctgctga tcggcggggc gacgaccagc aaggcgcata
     1321 ccgcgctccg catcgacccg gcctacgacg ggccggtgat ccacgtgctc gacgccagcc
     1381 gggcggtcgg ggtcgcctcg agcctggtct ccgacacgca agccgagccg ctggtcgcca
     1441 gcacggcgca agaatatgac agattgcgca agtcgcgcga gcgcagcggc cacagcgagc
     1501 tggcgactct ggaggaggcg cgcgccaacg ccttcccgtt cgaccccgac gggcagccgc
     1561 cgccgccgca gcgcccgggc ctccaccagt tcggcgaatg gccgctccgc gacctccgcg
     1621 cgtcgatcga ctggacgccc ttcttccgcg cgtgggagtt ggccggcaac tacccggcga
     1681 tcctcgacga cccggtggtc ggcgagagcg ccaagagcct gttcgcggat gcgcaggaca
     1741 tgctcgagaa gctgatcgcc gagcgctggg tgaccccgag cgcgaccgtc ggcctgtggc
     1801 gctgcaagcg cgaaggcgac gacgtgctcg ttctcgcggg caacgactgg acgcgcgtgc
     1861 ccttcctccg ccagcaggtg aagaagcgcg aggggaggcc caacatgtgc ctcgccgact
     1921 tcatcaaccc ggagggcgag gactggatcg gcggcttcgc ggtcgggatc cacgggctcg
     1981 agccgcatct cgagcggttc aaggccggca acgacgatta ttcggacatt ctgctgaaag
     2041 ccctcgccga ccggctggcc gagagcctcg ccgaagtgct tcacgccgaa gtccgcaacc
     2101 ggatctgggg ctatgccgac gagcatttct ccaacgagca gctgatccgt gagaaatatc
     2161 aggggattcg gccggcgccc ggctacccgg cgtgccccga ccacagcctg aagccgatgc
     2221 tgttcgacat gctcggcgga acgcccggcg gggtcaccct caccgaaaat tacgcgatgc
     2281 tgccgacctc cgcggtgtcg ggcttctatt tcggccaccg cgacgcccag tatttcggtg
     2341 tcgcgcggat cggccgcgac cagctcgagg attatgcgcg gcggcgcggg gtcggcatcg
     2401 agactgccga gcgctggctg cggcccaatc tcgactgatc cccgccgtca ttgcgagcgc
     2461 agcgaagcaa tccagcaatg gcgcgagccc gggaatggat tgcttcgtcg cttcgctcct
     2521 cgcaatgacg gtacggtgat gcttcgcttc tttctgctcc tgctcgcctt tctggccatg
     2581 ccgtccctgg ccgaggcacg cgaaaacgct atccgtcctc aactggtcgc ggaagggccg
     2641 gcgccgccgg gcggcgaggt cgagctggcg atcgacatgc ggacccggcc gggctggcac
     2701 ggctattggc gcaaccccgg cgacgccggc ctgccgatgg aagtcgactg gacgcttccg
     2761 cccggcttcg ccgtcgggcc gctgcgctac ccggtgccga cccggctgac catcgccggg
     2821 ctcatgaatt acgtctacga gcgcgattat gcggtgctgg tgcggctgaa ggtgccgccc
     2881 gatgcgcgcg gcagcgtccc gatccgcgcc gacgcgcgct ggctcgcgtg caccgacaag
     2941 gtctgcgtgc ccgaaagcgg ctctttcgcg ctgaccc
//
LOCUS       DASTZO010000057         5425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1101766,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000057 DASTZO010000000
VERSION     DASTZO010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5425
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1101766"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="VFU20_01515"
     CDS             <1..1235
                     /locus_tag="VFU20_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RelA/SpoT AH/RIS domain-containing protein"
                     /protein_id="HEU5285170.1"
                     /translation="QFVPGRFKDYISTPKTNGYRSLHTTLIYESSMRVEVQIRTQEMH
                     RLNEFGLAAHWAYKQGERPDGQVGWLRDLIEIVDASHDAEELLEHTRLAIYQDRIFAF
                     TPKGALFQLPKGATPVDFAYAVHTDLGDRTVGAKVNGRMVPLRTILENGDQVEILASE
                     AQHPQPAWLRFVATGKARAGVRRFVRHKEREETVELGRKIYDEIVERLPAMLDKDALK
                     RALKKLKLDDEDSLMAAIARKRISDEALMEALMPGSAGGDVAHKPLSQRTAISIKGLT
                     AGVAYHLATCCHPIPGDRIVGLRREDEEIEVHVIGCDTLASGVDADWLDLAWGEGSDG
                     GAARIAVILRDIPGALGAMSGILGSKHANIVNLQLVHRDGSFHTFHIDVEVHDLAHLH
                     AIIAALREADPISSVERI"
     gene            complement(1258..1893)
                     /locus_tag="VFU20_01520"
     CDS             complement(1258..1893)
                     /locus_tag="VFU20_01520"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase"
                     /protein_id="HEU5285171.1"
                     /translation="MLISRRHLVVGGAAMAGLAASAGARKLVPTSAQALGPFYPVVRP
                     RDMDADLTRVRGAKGVARGDPINVIGRVVDLRGRPVRGVRVELWQANAVGRYDHPGDH
                     ANPAALDPNFQGFARLSTDGDGHFKFRTIKPSAYNTPIGMRTPHIHFDIHGRSERLVT
                     QMYFPGEPLNEKDFLLRTAEPRESVIAEAIPRLSGDPGAAAFHWTVVLAYG"
     gene            complement(1949..4228)
                     /locus_tag="VFU20_01525"
     CDS             complement(1949..4228)
                     /locus_tag="VFU20_01525"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEU5285172.1"
                     /translation="MSESNVKFSEAEAIDFHARGRPGKIEIIASKPMATQRDLSLAYS
                     PGVAVPVRAIAEDPGRAYELTAKGNLVAVISNGTAILGLGNLGALAAKPVMEGKAVLF
                     KRFADVDSIDIEVDTEDCDKFIECVALLAPTFGGINLEDIAAPDCFVIEQALKERMNI
                     PVFHDDQHGTAIITAAGLINACYLTGRTLGDLKVVVNGAGAAAIACTELIKAMGVRGD
                     NVVMCDRKGVIYKGRGDVDQWKSAHAADTDARTLADALHGADVFLGLSAAGALKPEMV
                     AGMAKDPIIFAMANPDPEITPPEARAARPDAIIATGRSDYPNQVNNVLGFPFIFRGAL
                     DVRATAITDEMKIAAAKALAELAREAVPEEVAAAYGGQTHSFGRDYIIPAPFDPRLME
                     VVASAVAEAAMASGCAQKPIEDMRAYRQQLRARLNPTVAVMSLAYEAARSNPKRVLFA
                     EGEEPNVLRAAIAFKEAGYGTPVLVGREEVHDLLKGLGVEKPEDYEVLNSRNSPLVGR
                     AVDYIYAKHQRHGLLRREVERMVNQDRNTFTAAMLALGEGDAMITGTTRPFSQSLKQV
                     RMVIDEEPGATPFGVNVIVGRHHTVLIADTAVTERPDAEQYAAIAMRSSGFARRMGLE
                     PRVAFISYTTFGNPPGMHIEELRGAVKLLDGIHCDFEYEGEMGPDVALNFDMQQRFYP
                     FSRLTGPANILVMPGLQSANLSAKLLRELGGESVLGPYILGLELPVQIAPMTASASDL
                     VTLAVLAAGSARERVRPAG"
     gene            4302..>5425
                     /locus_tag="VFU20_01530"
     CDS             4302..>5425
                     /locus_tag="VFU20_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010407126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU5285173.1"
                     /translation="MARADEPTPMMAQYRRLKDEAGEALLFYRMGDFFELFFDDARVA
                     AACLDIALTRRGASEGEPIPMCGVPVHSAESYLARLIRAGHRAAIAEQVESPSEARKA
                     RGSKALVERAIVRLVTPGTLTEEALLESGAANWLAAVGRAGDEWAVAAADISTGRFEL
                     IACGPGDLAAELARLSPAETLADGAIPGVRTTVGKGGFDSIAGERALKRRFGLATLDG
                     LGSPSRAELAAAGGLLAYLDATQKGTGTLLDSPRRVARSSHVAIDAATRDSLELCRSV
                     SGTLAGSLLGEIDRCQTAAGRRLLAEDISAPLTDKPAIEARLALVGWLHADALRRERV
                     RAALKAMPDFARALARLAAQRGSPRDLALLRDGLSAAAAL"
BASE COUNT          799 a         1910 c         1895 g          821 t
ORIGIN      
        1 ggcagttcgt gcccggccgg ttcaaggact acatctcgac gccgaagacc aacggctacc
       61 gcagcctgca cacgacgctg atctatgaga gctcgatgcg cgtcgaggtg cagatccgca
      121 cgcaggagat gcaccggctc aacgagttcg gcctcgccgc gcactgggcc tacaagcagg
      181 gcgagcggcc cgacggccag gtcggctggc tgagggacct gatcgagatc gtcgacgcca
      241 gccacgacgc cgaggagctg ctcgagcaca cgcggctggc gatctaccag gaccgcatct
      301 tcgccttcac cccgaagggc gcgctgttcc agctgcccaa gggcgcgacc ccggtcgact
      361 tcgcttatgc ggtgcacacc gacctcggcg accgcaccgt cggggccaag gtcaacggcc
      421 gaatggttcc gctgcgaacc atcctcgaga acggcgacca ggtcgaaatc ctggcttcgg
      481 aggcccagca cccgcagccg gcgtggcttc gcttcgtcgc caccggcaag gcccgcgccg
      541 gggtccggcg cttcgtccgc cacaaggagc gcgaggaaac cgtcgagctc ggccgcaaga
      601 tctacgacga gatcgtcgag cggctgccgg cgatgctcga caaggacgcg ctcaagcggg
      661 cgctcaagaa gctcaagctg gacgacgagg attcgctgat ggcggcgatc gcccgcaagc
      721 ggatcagcga cgaggcgctg atggaggcgc tgatgcccgg ctcagccggc ggcgacgtcg
      781 cccacaagcc gctcagccag cgcaccgcga tctcgatcaa gggcctgacc gccggggtcg
      841 cctaccacct cgccacctgc tgccacccga tccccggcga ccggatcgtc ggcctgcgcc
      901 gcgaggacga ggagatcgag gtccatgtga tcggctgcga cacgctggcc agcggagtcg
      961 acgccgactg gctggacctg gcctggggcg agggctcgga cggcggggcg gcgcggatcg
     1021 cggtcatcct gcgcgacatc cccggagcgc tcggcgccat gtccggaatc ctcggctcca
     1081 agcacgccaa catcgtcaat ttgcagctgg tccaccgcga cggcagcttc cacaccttcc
     1141 acatcgacgt cgaggtccac gacctcgccc acctgcacgc gatcatcgcc gcgctccgcg
     1201 aggccgaccc gatcagctcg gtcgagcgga tctagccgtt tcgcgccacg aggcgggtca
     1261 gccgtaggcc agcaccaccg tccagtggaa ggcggcggcg ccgggatcgc ccgacaggcg
     1321 cggaatcgcc tcggcaatga cgctttcgcg cggttcggcg gtgcgcagga ggaagtcctt
     1381 ctcgttgagc ggctcgcccg ggaaatacat ttgcgtgacc agccgttcgg accgcccgtg
     1441 gatgtcgaaa tggatgtgcg gcgtgcgcat cccgatcggc gtattgtagg cgctcggctt
     1501 gatcgtccga aacttgaagt ggccgtcgcc gtcggtcgac aggcgcgcga agccctggaa
     1561 attgggatcg agcgccgccg gattggcgtg atcgccggga tggtcgtagc ggccgacggc
     1621 attggcctgc cacaactcga ccctgacacc gcggaccggg cgcccgcgaa ggtcgacgac
     1681 ccggccgatg acgttgatcg gatcgccccg ggcgactccc ttcgcgccgc gcacccgggt
     1741 caaatcggcg tccatgtccc gcggccggac gaccggatag aagggcccca gggcctgcgc
     1801 ggatgtcggc accagcttgc gcgctccggc gcttgccgcg agcccggcca tcgccgcccc
     1861 gccgaccact aggtgccgcc tgcttatcag catatcgagt ctccccctcg aggagactcg
     1921 ggtcattacg cctgcggcga agggcggtct agccggccgg ccgaacgcgc tccctcgcgc
     1981 tccccgcggc gagcaccgcg agggtcacca ggtccgaggc ggacgcggtc atcggcgcga
     2041 tctggaccgg cagctcgagc ccgaggatgt acggcccgag cacgctttcg ccgccgagct
     2101 cgcgaagcag cttggccgac aggttcgccg actgcaggcc cggcatgacc aggatgttgg
     2161 ccgggccggt cagccggctg aacgggtaaa aacgctgctg catgtcgaag ttgagagcga
     2221 cgtcgggccc catctcgcct tcatattcga agtcgcaatg gatcccgtcg aggagcttca
     2281 ccgccccgcg cagctcctcg atgtgcatcc cgggcgggtt gccgaacgtc gtgtagctga
     2341 tgaacgcgac ccgcggctcg agccccatcc ggcgggcaaa cccggacgag cgcatggcga
     2401 tcgccgcata ttgctcggca tcgggccgct cggtcaccgc ggtgtcggcg atcagcaccg
     2461 tgtggtgccg gccgacgatg acgttgaccc cgaacggagt cgcgcccggc tcctcgtcga
     2521 tgaccatccg cacctgcttc agcgactggc tgaacgggcg cgtggtgccg gtgatcatcg
     2581 cgtcgccctc acccaatgcg agcatcgccg cggtgaacgt gttgcggtcc tggttgacca
     2641 tccgctcgac ctcgcggcgc agcagcccgt ggcgctggtg cttggcgtag atgtaatcga
     2701 ccgcccgccc gaccagcggc gagttgcggc tgttgagcac ctcatagtct tccggtttct
     2761 cgaccccgag gcccttgagc aggtcgtgca cctcctcgcg gccgaccagc accggcgtcc
     2821 cgtagccggc ctccttgaag gcgatcgcgg cgcgcagcac gttgggctcc tcgccttccg
     2881 cgaacagcac ccgcttcggg ttcgagcgcg cggcttcgta ggcgaggctc atcaccgcga
     2941 cggtcgggtt cagccgcgcc cgcagctgct ggcggtaggc gcgcatgtcc tcgatcggct
     3001 tttgcgcgca gccgctcgcc atcgccgcct cggcgaccgc ggacgcgacc acctccatca
     3061 gccgcgggtc gaacggcgcc gggatgatat agtcgcgccc gaagctgtgg gtctggccgc
     3121 cataggccgc cgcgacctcc tcgggcaccg cctcgcgggc cagctcggcg agcgccttgg
     3181 cggcggcgat cttcatctcg tcggtgatcg cggtcgcccg cacgtcgagc gcgccgcgga
     3241 agatgaacgg gaagccgagc acgttgttga cctggtttgg atagtcggag cggccggtgg
     3301 cgatgatcgc gtcgggccgg gcggcgcgcg cttcgggcgg agtgatctcc gggtccgggt
     3361 tggccatcgc gaagatgatc gggtctttgg ccatcccggc gaccatctcg ggcttgagcg
     3421 ccccggccgc cgacagcccg aggaacacgt cggcgccgtg cagggcgtcg gccagcgtcc
     3481 gcgcgtcggt gtcggcggcg tgcgccgact tccactggtc gacgtcgccg cggcccttgt
     3541 agatgacgcc cttgcggtcg cacatgacga cgttgtcgcc gcgcaccccc atcgccttga
     3601 tcagctcggt gcaggcgatc gccgccgccc ccgcgccgtt gaccaccacc ttgaggtcgc
     3661 caagcgtccg cccggtcaga tagcaggcgt tgatcagccc ggcggcggtg atgatcgcgg
     3721 tgccgtgctg gtcgtcgtgg aacaccggga tgttcatccg ctccttcagc gcctgctcga
     3781 tgacgaagca gtcgggggcg gcgatgtcct cgaggttgat gccgccgaag gtcggggcga
     3841 gcaaagcgac gcattcgatg aacttgtcgc aatcctcggt gtcgacctcg atgtcgattg
     3901 aatcgacgtc ggcgaagcgc ttgaacagca ccgccttgcc ttccatcacc ggcttggcgg
     3961 cgagcgcgcc gagattgccg aggccgagga tcgcggtgcc gttggagatc accgcgacca
     4021 gattgccctt ggcggtcagc tcgtaggcgc ggcccgggtc ctcggcgatc gcacgcaccg
     4081 gcaccgcgac tcccggcgag taggctaggc tgaggtcgcg ctgggtcgcc atcggcttgg
     4141 acgcgatgat ctcgatcttc cccggccggc cgcgagcgtg gaagtcgatc gcctcggcct
     4201 cggagaattt gacgttgctc tcggacatgc cttgctccct ggcggcgggc tttagccaaa
     4261 gggtgtggga agagtcacgc ggagccgcta ccacgttggc gatggcccga gccgacgaac
     4321 cgacgccgat gatggcgcag taccggcgcc tcaaggacga ggccggcgaa gcgcttttgt
     4381 tctaccggat gggcgatttt ttcgagctgt tcttcgacga cgcaagggtc gccgcggcct
     4441 gcctcgacat cgccttgacc cggcgcggcg catccgaggg cgagccgatc ccgatgtgcg
     4501 gcgtgccggt ccattcggcc gaatcctatc tcgcccggct gatccgcgcc ggccaccggg
     4561 ccgcgatcgc cgagcaggtc gaaagcccga gcgaggcgcg caaggcgcgc ggctccaagg
     4621 cgctggtcga gcgggcgatc gtccggctgg tcacgccggg cacgctgacc gaggaggcgc
     4681 tgctcgaatc gggcgccgcc aactggctgg cggcggtcgg ccgggcgggc gacgaatggg
     4741 cggtcgccgc cgccgacatc tcgaccgggc ggttcgagct gatcgcctgc gggcccggcg
     4801 accttgccgc cgagctggcg cggctgtcgc cggcggagac cctcgccgac ggcgcgatcc
     4861 ccggcgtccg gacaaccgtc ggcaagggcg gcttcgacag catcgccggc gagcgcgcgc
     4921 tcaagcgccg cttcgggctg gcgacgctcg acgggctcgg ttcgccgtcg cgcgccgagc
     4981 tcgccgccgc cggcgggctg ctggcctatc tggacgcgac ccagaaaggc accggcacat
     5041 tgctcgactc gccgcggcgg gtcgcccggt cgagccatgt cgcgatcgac gccgccacgc
     5101 gggacagcct cgagctctgc cggtcggtgt ccgggacgct cgccggcagc ctcctgggcg
     5161 agatcgaccg ctgccagacg gccgccgggc ggcggctgct cgccgaggac atttccgcgc
     5221 cgctgaccga caagcccgcg atcgaggcgc ggctcgcgct ggtcggctgg ctgcacgcgg
     5281 acgcgctgcg ccgcgagcgg gtgcgcgccg ctctgaaggc gatgcccgat ttcgcccggg
     5341 cgctggcgcg gctggcggcg cagcgcgggt cgccacgcga cctcgctctg ctgcgcgacg
     5401 ggctgtcggc cgcggccgct ttgaa
//
LOCUS       DASTZO010000058         4679 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_501084,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000058 DASTZO010000000
VERSION     DASTZO010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4679)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4679)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4679
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501084"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /locus_tag="VFU20_01535"
     CDS             <1..1143
                     /locus_tag="VFU20_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5285174.1"
                     /translation="DKAIDVIDEVGAMQMLVPPSRRKKVITVKEIEAVVATMARIPPK
                     SVSTDDKRVLENLEADLKRVVFGQDLAIEKLASAIKLSRAGLRDPDKPIGNYLFSGPT
                     GVGKTEVARQLASIMGIPLQRFDMSEYMERHSVSRLIGAPPGYVGYDQGGLLTDAVDQ
                     HPHSVLLLDEIEKAHPDLFNILLQIMDHGKLTDHHGKTVDFRNAILIMTTNAGASDMA
                     REGIGFGTMDREDAQEDAVKKMFTPEFRNRLDAIVPFSYLPPAVVARVVDKFILQLEL
                     QLADRGVHIDLDDEARAWLTERGYDKLYGARPMGRLVQEKIKQPLAEELLFGKLVNGG
                     EVKVRVKDNAPAFEITPAPPKAAKAKAPKGGAKAKARSEKAPDAEE"
     gene            1204..2916
                     /locus_tag="VFU20_01540"
     CDS             1204..2916
                     /locus_tag="VFU20_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HEU5285175.1"
                     /translation="MTRIHWLAASAMALVAAPPLATQALAQTVSFDPARLSRHIQVIS
                     SDAYEGRAPATRGEQMTVDYLIREFKAAGLKPGGEVIDGKRQWTQRVPLLKSDLVGTP
                     QVVVQTPAGPMPLAQGEQVAVRSPTNGQQQVRLTGAPLIFAGYGVAAPERQWDDFKGQ
                     DVRGKVLVVLVNDPDFEGGEGNFDGKAMTYYGRWTYKYEEAARRGAAGVMIVHETEPA
                     SYGWATVKNSNTITMFDIVRANPAAEHTGFESWIQRDTAVALFRAAGLDFEAAKQAAR
                     RRDFQPVDLKSTLTASLDARTETITSFNVAGVLPGRTRPDETVIYTAHHDHLGIGQPD
                     ANGDRIFNGAVDNGTGIAHLIEQARAFARRPRTQRSVVFLAVGAEEKGLLGSEYYASN
                     PLYPLAKTAGVLNTDVMGTWGRARNFSIRGSARLDLLDRVIAEGARQGRRFTPEPNPG
                     AGSFFRSDHFPFAKVGVPAISFTPGNDLVNGGIARGDALAREYTAKRYHQQDDEFNRA
                     WDFSGMVQDAQLLHRVGYGLANSAEWPNWSDDSQFRSLRDESAGERGQAPPPPVPSPR
                     GERG"
     gene            2913..3416
                     /locus_tag="VFU20_01545"
     CDS             2913..3416
                     /locus_tag="VFU20_01545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HEU5285176.1"
                     /translation="MSETLEPALPDQLDRHAERLMRALCDRGLTIATAESCTGGMLAA
                     LLTDIEGAGHGFERGFVAYTKQAKADLLGIDPGLLDRNDAVSEAVARAIAEGALDRSS
                     ADIAIGVTGFAGPAGDGCEEGLVHFALARRGRPTRHRVEHFGPAGRGPVRVQSLRAML
                     EMLDEAL"
     gene            3450..4094
                     /locus_tag="VFU20_01550"
     CDS             3450..4094
                     /locus_tag="VFU20_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU5285177.1"
                     /translation="MSSLTFYTHPMSRGQTVRWMLEEVGEPYETQILDYGSTMKVEPY
                     LSINPMGKVPAIKHGDKVVTEVAAICCYLADAFPAAGLAPPPSERADYYRWIFFTSGP
                     VEAAFSNKAAGWEPTPERQRMFGYGTYDLAIGTLEKALTGRKYIAADHFTAADLFVGA
                     NVNFMLAFNLLEPRPVFTDYAARMTDRDAYRRAKEIDGQLIAEIQARQAQPQPA"
     gene            4171..>4679
                     /locus_tag="VFU20_01555"
     CDS             4171..>4679
                     /locus_tag="VFU20_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5285178.1"
                     /translation="MTDDLRAKAIALYDRFTHEGMDRRLFMRRMAALAGSAAAAEALV
                     GAIAASPAAAAIVPPDDRLLTTRTMTFAGPAGYRAYVAEPRTRSLKPTVLVIHENRGL
                     NDHIRDVARRLALTGFKAVAPDFLSPVGGTSANEDAARAAIGKLDLGAATASAVAMLD
                     ELAKGSRGG"
BASE COUNT          800 a         1684 c         1545 g          650 t
ORIGIN      
        1 gacaaggcga tcgacgtgat cgacgaggtt ggcgcgatgc agatgctggt gccgcccagc
       61 cgccgcaaga aggtcatcac cgtcaaggaa atcgaggcgg tggtcgcgac catggcgcgg
      121 attccgccca agtcggtctc gaccgacgac aagcgcgttc tcgaaaacct cgaggcggac
      181 ctcaagcgag tcgtgttcgg ccaggacctg gcgatcgaga agctggcgag cgcgatcaag
      241 ctgtcacgcg ccggccttcg cgaccccgac aagccgatcg gcaattatct gttctcaggc
      301 ccgaccggcg tcggcaagac cgaggtcgcc cgccagctcg cctcgatcat gggcatcccg
      361 ctgcagcgct tcgacatgtc cgaatatatg gagcgccact cggtcagccg gctgatcggc
      421 gctcctcccg gctatgtcgg ctacgaccag ggcgggctgc tgaccgatgc ggtcgaccag
      481 cacccgcaca gcgtgcttct gctcgacgag atcgagaagg cgcaccccga cctgttcaac
      541 atcctgctgc agatcatgga ccacggcaaa ctgaccgacc accatggcaa gacggtcgat
      601 ttccgcaacg cgatcctgat catgaccacc aacgccggcg cgtccgacat ggcgcgcgag
      661 ggcatcggct tcgggacgat ggaccgcgag gacgctcagg aggatgcggt caagaagatg
      721 ttcacgcccg agttccgcaa ccgcctcgac gcgatcgtcc ctttctccta cctgccgccg
      781 gcggtggtcg cccgggtcgt cgacaagttc atcctccagc tcgagctgca attggccgac
      841 cgcggggtcc acatcgacct cgacgacgag gcgcgcgcct ggctgaccga gcgcggctac
      901 gacaagctgt acggagcccg gccgatgggc cgcctggtgc aggagaagat caagcagccg
      961 ctcgccgagg aattgctgtt cggcaagctg gtcaacggcg gcgaggtcaa ggtccgggtc
     1021 aaggacaatg cgccggcgtt cgagatcacc ccggcgccgc ccaaggcggc caaggccaag
     1081 gcgcccaagg gcggcgccaa ggccaaagcg cgcagcgaaa aggcccccga cgcggaggaa
     1141 tgaaacctat tgcggctcgg ctcgtcttgc ccgcgaaggc atcgagcagg gagtaggttt
     1201 caaatgactc ggatccactg gctggccgca agcgcgatgg cgctcgtcgc ggctccgccc
     1261 cttgccaccc aagcgctggc gcagaccgtc tcgttcgacc cggcgcggct gtcgcgccac
     1321 atccaggtga tcagctcgga cgcttatgaa ggccgtgccc cggcgacccg cggcgagcag
     1381 atgacggtcg attatctgat ccgcgagttc aaggcggccg gcctcaagcc cggcggcgag
     1441 gtgatcgacg gcaagcgcca gtggacccag cgcgtgccgc tgctcaaatc cgacctcgtc
     1501 ggcaccccgc aggtcgtcgt ccagaccccc gccgggccga tgcctctggc ccagggcgag
     1561 caggtcgcgg tccgctcgcc gaccaacggc cagcagcagg tccggctcac cggcgcgccg
     1621 ctcattttcg ccggctacgg ggtcgccgcg cccgagcgcc agtgggacga tttcaagggc
     1681 caggacgtgc gcggcaaggt cctcgtcgtg ctggtcaacg accccgactt cgagggcggc
     1741 gaaggcaatt tcgacggcaa ggcgatgacc tattacggcc gctggaccta caaatatgag
     1801 gaagcggcgc ggcgcggcgc cgccggggtg atgatcgtcc acgagaccga gcccgcctcc
     1861 tacggctggg cgacggtcaa gaacagcaac accatcacca tgttcgacat cgtccgggcc
     1921 aacccggcgg ccgagcacac ggggttcgaa agctggatcc agcgcgacac ggcggtcgct
     1981 ttgttccgcg ccgcggggct cgacttcgag gccgcgaagc aggccgccag gcgccgcgac
     2041 ttccagcccg tcgacctcaa gtcgacgctc accgccagcc tcgacgccag gaccgagacc
     2101 atcacctcct tcaatgtcgc cggggtcctg ccgggcagga cgcgccccga cgagacggtg
     2161 atctacaccg cgcaccacga ccatctcggc atcggccagc ccgacgccaa cggcgaccgc
     2221 atcttcaacg gcgcggtcga caacggcacc ggcatcgccc atctgatcga gcaggcgcgc
     2281 gccttcgccc gccgcccgcg cacccagcgc tcggtggtgt tcctcgccgt cggcgccgag
     2341 gagaagggcc tgctgggctc cgaatattat gcctcgaacc cgctctaccc gctggccaag
     2401 acggccggtg tgctcaacac cgacgtgatg ggcacctggg gccgggcgcg caacttcagc
     2461 atccgcggca gcgcccggct cgacctgctc gaccgggtga tcgccgaggg cgccaggcag
     2521 gggcggcgat tcacccccga gccgaacccc ggcgccggca gcttcttccg ctccgaccat
     2581 ttcccgttcg ccaaggtcgg ggtgccggcg atttccttca cgcccggcaa cgacctggtc
     2641 aacggcggga tcgcccgcgg cgacgcgctc gcccgcgaat ataccgccaa gcgctaccac
     2701 cagcaggacg acgagtttaa ccgcgcctgg gatttctccg gcatggtcca ggacgcgcag
     2761 ctgctccacc gggtcggcta cggcctcgcc aattcggccg aatggcctaa ttggagcgac
     2821 gacagccagt tccgctcgct gcgcgacgag agcgccggcg agcgcggcca agcgccgccg
     2881 ccgccagtcc cgtctccaag gggagagcgc ggttgagcga aacgctcgag ccggcgcttc
     2941 ccgaccagct cgaccgccac gccgaacggc tgatgcgcgc gctgtgcgac cgcgggctga
     3001 ccatcgccac cgcggaaagc tgcaccggcg ggatgctcgc ggcgcttctg accgacatcg
     3061 agggcgccgg ccacggcttc gagcgcggct tcgtcgccta caccaagcag gccaaggccg
     3121 acctgctcgg gatcgacccc gggctgctcg accgcaacga cgcggtgtcg gaagccgtcg
     3181 ctcgggcgat agccgagggc gcgctcgacc gctccagcgc cgacatcgcc atcggggtca
     3241 ccggcttcgc cggccccgcc ggcgacggct gcgaggaagg gctggtccat ttcgccctcg
     3301 cccgccgcgg ccggccgacc cgacaccggg tcgagcattt cggccccgcg ggccgcggcc
     3361 cggtgcgagt ccagtcgctg cgggcaatgc tggagatgct cgacgaagcg ctctaatagg
     3421 gccgcaatcg gccgacggag gagcagcgca tgagcagcct caccttctac acccacccga
     3481 tgtcgcgcgg gcagacggtc cgctggatgc tcgaggaggt cggcgagccc tacgagacgc
     3541 agatcctcga ctatggctcg acgatgaagg tcgagcccta tctgtcgatc aacccgatgg
     3601 gcaaggtccc ggcgatcaag cacggcgaca aggtggtgac cgaggtcgcg gcgatctgct
     3661 gctacctcgc cgacgccttc cccgccgccg gcctggcgcc gccgccgtcg gagcgcgccg
     3721 attactatcg ctggatcttc ttcacctcgg gcccggtcga ggcggcgttc agcaacaagg
     3781 cggccggctg ggagccgacc cccgagcgcc agcgcatgtt cggctacggc acgtacgacc
     3841 tcgccatcgg cacgctcgag aaagcgctga ccgggcgcaa atatatcgcc gcggaccatt
     3901 tcaccgccgc cgacctgttc gtcggcgcca acgtcaactt catgctggcc ttcaacctgc
     3961 tcgagcccag gccggtgttt accgattatg cggcgcggat gaccgaccgc gacgcctacc
     4021 gccgagccaa ggagatcgac ggccagctga tcgccgagat ccaggcccgg caggcgcagc
     4081 cgcagccggc gtgagttccg ccccggcatt tgccggggag ggggaccatc caacgcagat
     4141 tggatggtgg aggggttttt ggaggtgcca atgaccgacg acctccgcgc aaaagcgatc
     4201 gcgctctacg accgcttcac ccacgagggc atggaccgac gcctgttcat gcgccgcatg
     4261 gccgcgctgg ccggaagcgc cgccgccgcc gaggcgttgg tcggggcgat cgccgcctcc
     4321 cccgcggcgg ccgcgatcgt ccctcccgac gaccggctgc tcacgacccg gacgatgacc
     4381 ttcgccggcc cggccggcta ccgcgcctat gtcgccgagc cgcgcacgcg ctccttgaag
     4441 ccgacggtgc tggtgatcca cgagaaccgc gggctcaacg accatatccg cgacgttgcc
     4501 cggcgcctcg ctttgacggg cttcaaagcg gtcgcccccg acttcctttc gccggtgggc
     4561 ggcacttcgg cgaacgagga tgcagcgcgc gcggcgatcg gcaagctcga cctcggcgcc
     4621 gcgacggcga gcgcggtggc gatgctcgac gagctggcca agggcagccg cggcggcaa
//
LOCUS       DASTZO010000059         4541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_135776,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000059 DASTZO010000000
VERSION     DASTZO010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4541
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135776"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="VFU20_01560"
     CDS             complement(<1..1587)
                     /locus_tag="VFU20_01560"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5285179.1"
                     /translation="MGTGVAEPGRRVTLRMLRLAAAPLALLLSGAAVPAGQAAADRAA
                     ADRVRGHVEFLASDELEGRDTGSRGHAVAARYVAAQFAGLGLKPGGTGGSWYLQVPFR
                     RASHSSPPQVRLRVGGRTTALTHGIDVALQPSLLEQSRSIDAALVFAGYGLDEPKLGL
                     DDYAGLDVRGKIVVAFSGSPAGLPTDVRAHLDGSKPEAAARRGAIGLVEISAEPTGAP
                     RRFGSMGRERLDWVDAAGRTGTASLLRARLALTAKASEQLFEGARTPLATLRGHARAR
                     RPMRGFELAARLSIDARSSWQDFSSPEVIGLLPGSDPRLAAEHVVLMGHLDHLGVKSD
                     SKPGEDAIYNGALDNAAGIATMLEAAREFAYSGRPPRRSILFIANTGEERGLLGADYF
                     AAHPTVPIRQIVAAVDLDMPLLLYDFTDVVAFGADHSTVARTVAQAARGMGIAVSSDP
                     MPEQSIFVRSDHYQFVRRGVPAILLFTGYANGGKARWDDFFARVYHTPRDDLSQPLNW
                     NAGARYAKLNYAIARALADAD"
     gene            complement(1551..2552)
                     /gene="galE"
                     /locus_tag="VFU20_01565"
     CDS             complement(1551..2552)
                     /gene="galE"
                     /locus_tag="VFU20_01565"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEU5285180.1"
                     /translation="MSKVPVLVTGGAGYIGSHAVLALREAGWPVAVIDDLSNGTRSVV
                     PGDVAFFEGSIADGRLVERALGEQRIAAILHFAGSIVVPESVEKPLDYYANNTLASHA
                     LIAAAVAAGVPHIVFSSTAAVYGAPERVPVEEDDPKQPINPYGASKWMTERMLADAAA
                     AHPFNYCALRYFNVAGADPQMRAGQIGKGSTHLIKVAVEAAVGKRDHVAVFGTDYPTP
                     DGTCVRDYVHVSDLADAHVAALERLIERPEENLVLNCGYGRGLSVLEVLDSLDRVAGK
                     PVPKRAMPRRPGDPPQLVASNRALVETLDWSPRFADIDTIVTHALEWERVLPSRAAA"
     gene            complement(2609..>4541)
                     /locus_tag="VFU20_01570"
     CDS             complement(2609..>4541)
                     /locus_tag="VFU20_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEU5285181.1"
                     /translation="LERMIQHHEGAVKMAEELLKQSGSAYDPVLFDFVNEVINDQKAE
                     IKRMSALVPGLVDDPRASLAAGFRDAGQAIRNLALVATLPKPTGFFDPANPAGAPPPK
                     PARKKTAADKKADADPRSPLLSFANTDMAFAGDLLAVGNYHGFNLYRLEAGGAPRLLS
                     SVICPGGQGDLSIVGDLLIMSVEQTRGRVDCGRQGISEDVSPERFRGLRIFDIRDRSR
                     PVQVGQVQTCRGSHTHSVVSGPDSAGTIIVYVSGTSTVRKEEELKGCVGDVPGDVRTA
                     LFRIDVVEIPVADPSRARIVDSPAVFADPQSGSLAGLWRGGHHGEGTQEASRTDECHD
                     ITVFPSRSLAAGACSGNGIIFDIADPRKPRRLDAVVDPGFAYWHSATFNNDGTKVLFT
                     DEWGGGSRPRCRVQDPRNWGANAVYDIVEGKLRFRSHYKLPAPQTEQENCVAHNGSII
                     PVPGRDIFVQAWYQGGISVIDFTDSARPVEIAFFDRGPIDGEQLVMGGFWSAYWYRGR
                     IYGTEIARGLDVLALKPSEHLSANEIAAAELADQGGQFNPQQQFPVTWPDEPVVALAY
                     VDQLQRTQALPGAAVAELTRALGGASARLAARQPDAGLAARLEALAEQVGAAASDAAS
                     QRRLAALRQSLRGIAARLR"
BASE COUNT          660 a         1599 c         1648 g          634 t
ORIGIN      
        1 gtcggcgtcg gcgagcgccc gggcgattgc gtagttgagc ttcgcgtagc gcgccccggc
       61 gttccagttg agcggctggg aaagatcgtc gcggggcgtg tgatagaccc gggcgaagaa
      121 atcgtcccac cgcgccttgc cgccgttggc atagccggtg aacaggagga tcgccgggac
      181 cccgcggcgg acgaactggt aatggtcgga gcggacgaag atcgactgct cgggcatggg
      241 atcggacgag accgcgatcc ccatcccccg ggccgcctgc gcgacggtcc tggcgacggt
      301 cgaatgatcg gccccgaacg cgaccacgtc ggtgaaatcg tagagcagca gcggcatgtc
      361 gaggtcgacc gcggcgacga tctgccggat cgggaccgtc gggtgagcgg cgaaataatc
      421 ggctccgagc agcccgcgct cctcgccggt gttggcgatg aacaagatgg agcgccgcgg
      481 cggccggccg gaataagcga actcgcgcgc cgcctcgagc atggtcgcga tcccggccgc
      541 attgtcgagc gcgccgttgt agatcgcgtc ctcgcccggc ttggagtccg acttcactcc
      601 gaggtggtcg aggtggccca tcagaaccac atgttccgcg gcgagccgag ggtcgctgcc
      661 gggcagcagc ccgatcactt cggggctcga gaagtcctgc cagctgctgc gggcatcgat
      721 cgacagccgc gccgcaagct cgaagccgcg catcggccga cgggctcgtg cgtggcctcg
      781 gagggtcgcg agcggcgtcc gggcgccctc gaacagctgc tcggaagcct tggcggtcag
      841 cgccaaccgg gcgcgcagca gcgacgccgt cccggtccgg ccagcggcgt ccacccagtc
      901 gaggcgctcg cggcccatcg acccgaagcg ccgcggagcg ccggtgggct cggcggaaat
      961 ctccaccagc ccgatcgcgc cgcgccgcgc cgcggcctcg ggcttggagc cgtcgaggtg
     1021 cgcccggaca tccgtcggaa ggccggccgg cgagcccgag aaagcgacga cgatcttgcc
     1081 gcgcacgtcg agcccggcat aatcgtcgag gccgagcttc ggctcatcca gcccatatcc
     1141 ggcgaacacc agggccgcgt cgatcgaccg gctctgctcg agcaggctcg gctgcaacgc
     1201 gacgtcgatg ccgtgggtca gcgcggtggt ccggccgccg acccgaagcc ggacctgcgg
     1261 cgggctcgaa tggctcgccc gccgaaacgg cacctgcagg taccagcttc cgccggtccc
     1321 gcccggcttc aggccgaggc cggcgaactg ggcggcgacg tagcgcgcgg cgaccgcatg
     1381 gccgcgcgag ccggtgtcgc gcccctcgag ctcgtcgctg gcgaggaact cgacatggcc
     1441 ccggacccgg tcggccgccg cccggtcggc cgccgcctgc ccggcgggga cggccgcgcc
     1501 cgaaagcagc agcgcgagcg gcgccgcggc gaggcggagc atccggagcg tcacgcggcg
     1561 gcccggctcg gcaacacccg ttcccattcg agcgcgtggg tgacgatcgt gtcgatgtcg
     1621 gcaaagcgcg ggctccagtc gagcgtctcg accagcgccc ggttggacgc gaccagctgc
     1681 ggcgggtcgc ccgggcggcg cggcattgcg cgcttcggca ccggcttgcc ggcgacccgg
     1741 tcgagcgaat cgagcacctc gagcaccgac aggccccgcc cgtagccgca gttgaggacg
     1801 aggttctcct cgggccgctc gatcagccgc tcgagcgccg cgacatgcgc gtcggcgagg
     1861 tcgctgacat ggacatagtc gcggacgcag gtgccgtcgg gcgtcggata gtcggtgccg
     1921 aacaccgcga catggtcgcg cttgccgacc gccgcctcga ccgcgacctt gatcagatgg
     1981 gtcgatccct tgccgatctg cccggcgcgc atctgcggat cggccccggc gacgttgaaa
     2041 tagcgcagcg cgcaataatt gaacggatgg gcggccgcgg cgtcggcgag catccgctcg
     2101 gtcatccact tggacgcgcc ataggggttg atcggctgct tggggtcgtc ctcctcgacc
     2161 ggcacccgct cgggagcgcc gtacaccgcg gcggtcgagg agaagacgat gtgcggcacc
     2221 ccggcggcga ccgcggcggc gatcagggcg tggctggcaa gcgtgttgtt ggcgtaatag
     2281 tcgagcggtt tctcgaccga ttccgggacc acgatcgagc ccgcgaaatg caggatcgcg
     2341 gcgatccgct gctcccccag cgcccgctcg accagccgcc cgtcggcgat cgacccctcg
     2401 aagaacgcga cgtcgccggg caccacggac cgggtgccgt tggacaggtc gtcgatcacc
     2461 gcgaccggcc agccggcctc gcgcagggcg agcaccgcgt ggctgccgat atagccggcg
     2521 ccgccggtga ccaggactgg gaccttgctc atccgggctc gctaacggag ccgccgcccg
     2581 agcgccaccc cgcgcggccg cggctcagct agcggagcct cgcggcgatc ccccgcaggc
     2641 tttgccggag cgccgccaag cgcctttgcg aggcggcgtc ggaagcggcg gccccgacct
     2701 gctccgccag ggcctccagc cgcgccgcga gcccggcgtc cggctggcgc gcagcgagcc
     2761 gcgccgaagc cccgccgagc gcccgggtca gctcggcgac ggcggcgccc ggaagcgcct
     2821 gggtccgctg cagctggtcg acgtaggcga gcgcgaccac cggctcgtcc ggccaggtga
     2881 ccgggaactg ctgctgcggg ttgaattggc cgccctggtc ggcgagctcg gcggcggcga
     2941 tttcgttggc gctcagatgc tcgctcggct tcagcgccag gacgtcgagg ccgcgggcga
     3001 tctcggtgcc gtagatccgg ccgcgatacc aataggccga ccagaagccg cccatcacca
     3061 gctgctcgcc gtcgatcggc ccgcggtcga aaaaggcaat ctcgaccgga cgcgcggaat
     3121 cggtgaagtc gatgacgctg atgccgccct ggtaccaggc ctggacgaag atatcgcggc
     3181 ccgggaccgg gatgatcgat ccgttgtggg cgacgcaatt ctcctgctcg gtctgcggcg
     3241 cgggcagctt gtagtggctc cggaaccgca gcttgccctc gacgatgtcg tagaccgcgt
     3301 tggcgcccca gttgcgcggg tcctgaaccc ggcaccgcgg ccggctgccg ccgccccatt
     3361 cgtcggtgaa cagcaccttg gtgccgtcgt tgttgaaggt cgccgaatgc cagtaggcga
     3421 agcctggatc gaccaccgcg tcgagccggc gcggcttgcg gggatcggcg atgtcgaaga
     3481 tgattccgtt gcccgaacag gcgcccgccg ccagcgagcg gctcgggaag acggtgatgt
     3541 cgtggcactc gtcggtccgg ctcgcttcct gcgtgccttc gccgtggtgg cctccgcgcc
     3601 acaggccggc gaggctgccg gactgcgggt cggcgaacac cgccgggctg tcgacgatcc
     3661 gggcgcgcga cgggtccgcg accgggatct cgacgacgtc gatccggaac agggcggtgc
     3721 gcacgtcgcc ggggacatcg ccgacgcacc ccttgagctc ctcctccttg cggaccgtcg
     3781 aggttcccga gacgtagacg atgatcgtcc cggcggagtc cggccccgac accaccgaat
     3841 gggtgtgcga gccgcggcag gtctgcacct ggccgacctg caccgggcgc gagcggtcgc
     3901 ggatgtcgaa gatccgcagc ccccggaagc gctccgggct gacgtcctcg ctgatgccct
     3961 gccgcccgca atcgacccgg ccgcgggtct gctcgaccga catgatcagc aggtcgccga
     4021 cgatcgacag gtcgccctgc ccgcccgggc agatgaccga gctcagcagc cgtggcgctc
     4081 ccccggcctc gagccggtaa aggttgaagc cgtggtaatt gccgacggcg agcaggtcgc
     4141 cggcgaacgc catgtcggtg ttggcgaagc tcagcagcgg cgagcggggg tcggcgtcgg
     4201 ccttcttgtc cgcggcggtc ttcttcctgg ccggcttcgg gggcggcgcc ccggcggggt
     4261 tggcgggatc gaagaagccg gtcggcttgg gaagggtggc gaccagcgcc aggttgcgga
     4321 ttgcctggcc ggcgtcgcgg aagccggcgg caaggctcgc ccgggggtcg tccaccagcc
     4381 ccgggaccag cgcgctcatc cgcttaatct cggccttctg gtcgttgatc acctcgttga
     4441 cgaaatcaaa caggaccggg tcgtaggccg agcccgactg cttgagcagc tcctcggcca
     4501 ttttgaccgc gccctcgtga tgctggatca tccgctcgag g
//
LOCUS       DASTZO010000060         6267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_292446,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000060 DASTZO010000000
VERSION     DASTZO010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6267
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292446"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..553
                     /locus_tag="VFU20_01575"
     CDS             <1..553
                     /locus_tag="VFU20_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285182.1"
                     /translation="RRGQWTAFRMYADPDAVMFNPQVVWAHEFLAGRKDPPVSVRWWP
                     AASYVSCDGRIAVNTGPWVRDGGKSVGYFTTVWQRERRRWRWVYDAGDTLKVARPVPR
                     KPFVRRASCRGKPTAPPLLALSPTAKRRGKGPPDDYGIGRSADSTLAWDWKVDSKGQR
                     KFRTFLWNGERFEQVVYDATVSQ"
     gene            564..1190
                     /locus_tag="VFU20_01580"
     CDS             564..1190
                     /locus_tag="VFU20_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEU5285183.1"
                     /translation="MIELFGSALVTFLVIIDPPGCAPIFASLTRGTSAAHRRAMAVRS
                     SIIAWAVLMFFALLGRPMLHALGISLASFKIAGGVMLFMIALDMVFERRTQRREERAQ
                     SIEGTPQAEDISVFPMAIPMISGPGSIASAMLWVSRADDTAEVLTVLAAITVVILITL
                     VTLLAAGPLMRLLGDRVEAMITRILGVILAALAAQFIVDGLKQSFPSL"
     gene            complement(1230..3029)
                     /locus_tag="VFU20_01585"
     CDS             complement(1230..3029)
                     /locus_tag="VFU20_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcrB/AcrD/AcrF family protein"
                     /protein_id="HEU5285184.1"
                     /translation="MSERKDQLDDNQALKLLERHWKWVTLAAWVILAAWFAYNRWAQI
                     HAFTLVDTDDNMRISQVRALLAGQDWFDLRQYRLNPPAGADIHWSRLVDLPLAGLILL
                     LRPLVGGADAERIAVAVAPLLPYLLLLFGLALTARRLVHPAAYALCFVALVSAGSTNG
                     MFMPTRIDHHGWQLALLSIAVAGLADPKRARGGATLGVASALSLAIGLELLIYLALAA
                     AATVLFWIDDRDEKRRLAAYAATLAGGTALAFLLFASDANRQAVCDALSPVWLSNALL
                     GGALLFAIAFASPADWKRRFALAAGAAVVVAAFHALAWPHCLTRLEGVSPEVRQLWLD
                     NVREARPIYRHGWHTATLIAALPITGLIGWALLAIRNRGDRALFRRTLAAAAPALAAA
                     ALLLWQTRTGPAAQMLSIVGAIAILWVLAPLARTIRRPVLQTAAIIVLAMIGLGAMVP
                     TAMQLAPARKQTARDIAIGRANRACNSLPALRPIARQPKGMVFTFVDLGPRIITVTHH
                     DAVIGPYHRNGEQIADVMKTFRGSESNARATLAKYRADYLLICPKSSTTTIFMAAAPK
                     GFYGQLNRGRVPAWLEPVELPKGSPFRMWRVAR"
     gene            3078..3503
                     /locus_tag="VFU20_01590"
     CDS             3078..3503
                     /locus_tag="VFU20_01590"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU5285185.1"
                     /translation="MIRISSLLPLDAERRVVATQAARYALAGMIITLLVAASYWAIAE
                     YLEVDPMLSLTIVFVFFTVVSFFAHGEFSFRGHGERDRQHVRLLRFLVVNLIGFASNQ
                     FFVWLLVKKLGGPNWWPVIPIIFVTPVLTFALHRRWVYK"
     gene            3500..4450
                     /locus_tag="VFU20_01595"
     CDS             3500..4450
                     /locus_tag="VFU20_01595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5285186.1"
                     /translation="MSVVILSVIVPVKDEQDAIEPFVARVRAVIEALPDPASGSFEIL
                     FIDDGSTDATMEKIRAAHAADKRVRAVSLSRNFGKEAALSAGLDHARGDAIVPMDADL
                     QDPPELLGEMLAKWREGFEIVYGVRNNRESDSLPKRLTADLYYRAHNWLSADKIPEHA
                     GDFRLLDRKVADVIRQMPERTRFMKGLFAWAGFRQASVSYTREPRRIGKTKFDYWKLW
                     TFALDGITSASTVPLRVWSYLGGIVALFAIGYALFVVVRTLISGVQVPGYASLMVAVL
                     FLGGLQLLSLGILGEYVGRIMIETKNRPLYVIRERVGGDE"
     gene            4451..5182
                     /locus_tag="VFU20_01600"
     CDS             4451..5182
                     /locus_tag="VFU20_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5285187.1"
                     /translation="MEAAVFQRMAELDESHWWFVGRRRILDQLIRREVRPPPGARILE
                     VGCGTGHNLEMLGRFGTVEATELNDSARALASERLGRPVKRAALPDLSMLADERYDLV
                     ALLDVLEHVADDQAALRAIRSKLRPGGKLLVTVPGNPWMWSAHDVSHHHHRRYRRREL
                     ERVATAAGLEIDLLSPFNSLLFPAIAAARIAGRLLGRESADDAMPPPPVNQLLRAIFE
                     MEAALIGRVPFPFGVSLAAVLRRPD"
     gene            complement(5118..>6267)
                     /locus_tag="VFU20_01605"
     CDS             complement(5118..>6267)
                     /locus_tag="VFU20_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285188.1"
                     /translation="ALAFLAFGLWLRLGRLDRTRLRALLFVPISFIVFFAHTFGWGTL
                     GLLCFSAEAVRQHDRGAGWRRSGMWAALHAMVMALPALVILAWRSNIEGPASREWFAW
                     NLKLEALLGVLRDRWMTFDWLSLAVVALVILYAIFSRRLTLSRNLAFSALVLLAGFLL
                     LPWVIFSSAYADMRLLPFVFAVAILAIRFRRETHLPTARVLAVLGLAFFLVRIGANTV
                     SLAMAADDQRSKLAALEHVPMGARVASLTGRGCGREWPMARNAHLGAMVIVRRHGFSN
                     DQWVTPGLNLLRLRNRDAGAFSADPSQIVRPNGCRPRSWRIDRALGALPREHFDYLWL
                     IQPPPYDPRRVAGMQRVWSGPGSQLFRVNPGGAGRPPATPRTGTAPGR"
BASE COUNT          979 a         2116 c         2240 g          932 t
ORIGIN      
        1 gcgccgcggc cagtggaccg cgttccggat gtacgccgac cccgacgcgg tgatgttcaa
       61 cccgcaggtg gtgtgggcgc acgagtttct cgccggcagg aaggatccgc cggtgtcggt
      121 ccgctggtgg cccgccgcga gctatgtgtc gtgcgacggc cggatcgcgg tcaacaccgg
      181 gccgtgggtg cgcgacggcg gcaagagcgt cggctatttc accaccgtct ggcagcgcga
      241 gcggcggcgc tggcgctggg tctatgacgc cggcgacact ctgaaagtcg cgcgcccggt
      301 gccgcgcaag ccgttcgtcc gccgggcgtc gtgccgcggc aagccgaccg ctccgccgtt
      361 gctcgccttg tcgccgacgg cgaagcgccg cggcaagggg ccgcccgacg attacggcat
      421 cggccggtcg gccgattcga cgctggcgtg ggactggaag gtcgattcga aggggcagcg
      481 caagttccgg acgttcctgt ggaacggcga gcgcttcgag caggtcgtct acgacgcgac
      541 cgtttcgcaa tgatcgggcg gcaatgatcg agctgttcgg gtcggcgctg gtcaccttcc
      601 tggtgatcat cgacccgccc ggctgcgcgc cgatcttcgc cagcctgacc cgcggcacgt
      661 cggccgccca ccgccgggcg atggcggtgc gatcgtcgat catcgcctgg gcggtgctga
      721 tgttcttcgc gctgctcggg cggccgatgt tgcacgcgct cgggatcagc ctcgccagct
      781 tcaagatcgc cggcggggtg atgctgttca tgatcgcgct cgacatggtg ttcgagcgcc
      841 gcacccagcg ccgcgaggag cgcgcccagt cgatcgaggg cactccgcag gccgaggaca
      901 tttcggtctt cccgatggcg atcccgatga tcagcgggcc cggatcgatc gccagcgcga
      961 tgctgtgggt gtcgcgcgcc gacgacaccg cggaagtgct gaccgtgctc gccgcgatca
     1021 ccgtggtgat cctgatcacg ctggtcaccc tgctcgccgc cgggccgctg atgcgcctgc
     1081 tcggcgaccg cgtcgaagcg atgatcacgc ggatcctcgg ggtgatcctc gccgcgctcg
     1141 ccgcgcagtt catcgtcgac gggctgaagc agagcttccc gagcctctag cgagctggcc
     1201 gcccgctggc cgggaagctt cccgagcctc tagcgggcga cccgccacat ccggaacggc
     1261 gaacccttgg gcagctcgac cggctcgagc caggcgggca cccggccgcg gttcagctgt
     1321 ccgtagaagc ccttgggggc ggcggccatg aagatcgtcg tcgtcgagct cttcgggcag
     1381 atcagcagat aatcggcgcg gtatttggcc agcgtcgccc gggcgttcga ctcgcttccg
     1441 cggaaggtct tcatgacgtc ggcgatttgc tcgccgttgc ggtgataggg gccgatcacc
     1501 gcgtcgtgat gggtgacggt gatgatccgc gggccgaggt cgacgaaagt gaacaccatg
     1561 cctttgggct gccgggcgat gggccgcagc gcgggcagcg aattgcacgc gcggttggcc
     1621 cggccgatgg cgatgtcgcg cgcggtctgc ttcctggccg gcgcgagttg catcgcggtc
     1681 gggaccatcg cccccaggcc gatcatcgcc aggacgatga tcgccgcggt ctgcagcacc
     1741 ggccggcgaa tggtccgggc gagcggcgcc agcacccaca ggatggcgat cgctccgacg
     1801 atgctcagca tctgcgccgc tggcccggtg cgggtctgcc acagcaatag cgcggccgcg
     1861 gcgagcgccg gcgcggccgc cgccagggtc cgccggaaca gggctcggtc gccgcgattg
     1921 cggatcgcca gcagcgccca gccgatcagc ccggtaatcg gcagagcggc gatcaacgtc
     1981 gcggtgtgcc agccgtggcg atagatcggc cgggcctcgc ggacattgtc gagccacagc
     2041 tggcggactt cgggcgacac gccttcgagg cgcgtcaggc aatgcggcca tgccagagcg
     2101 tggaacgcgg cgacaacgac cgccgctccg gccgccaggg cgaaacggcg cttccagtcg
     2161 gccggggagg cgaaggcgat cgcgaacagc agcgcgccgc cgagcagggc gttcgacagc
     2221 cacaccggcg acaaggcgtc gcacaccgcc tggcggttgg cgtccgacgc gaacagcagg
     2281 aaagcgagcg cggtcccgcc ggcgagggtc gccgcataag ccgcgaggcg gcgcttttcg
     2341 tcccggtcgt cgatccagaa cagcaccgtc gccgcggcgg cgagcgcgag gtagatcaaa
     2401 agctcgagcc cgatcgccag cgacaaagcg ctcgccaccc cgagcgtcgc tccgccgcgc
     2461 gcccgcttcg ggtcggcgag gccggcgacg gcgatgctga gcagagcgag ctgccagccg
     2521 tggtggtcga tgcgcgtcgg catgaacatg ccgttggtcg agccggcgct gaccagggcg
     2581 acgaagcaca gagcgtaggc cgccgggtgg accaggcggc gggcggtcaa cgcgagcccg
     2641 aacagcagca gcaggtaggg cagcagcgga gcgacggcga cggcgatgcg ctcggcgtcg
     2701 gcgccgccga ccaaaggccg gagcagcagg atcagcccgg cgagcggcag gtcgaccagc
     2761 cgcgaccagt ggatgtcggc gcccgccggc gggttgagcc ggtactggcg aaggtcgaac
     2821 cagtcctgcc cggcgagcaa ggcgcgcacc tggctgatgc gcatattgtc gtcggtgtcg
     2881 accagcgtga aggcgtggat ctgcgcccag cggttgtagg cgaaccaggc ggcgaggatg
     2941 acccaggcgg ccagggtcac ccatttccag tggcgctcga gcagcttgag cgcctggttg
     3001 tcgtccagct gatccttgcg ctcgctcact tttcgcgctc tagccgctcg acctcaaagg
     3061 gcaagcagcg gccggcagtg atccgtattt cctccctcct cccgctcgat gccgaacgcc
     3121 gcgtggtcgc gacccaggcc gcccgctacg cgctggccgg aatgatcatc accctgctgg
     3181 tcgcggcaag ctattgggcg atcgccgaat atctcgaggt cgacccgatg ctgtcgctga
     3241 ccatcgtctt cgtcttcttc accgtcgtca gctttttcgc gcacggcgag ttcagcttcc
     3301 gcggccacgg cgagcgcgac cgccagcacg tgcggctgct ccgcttcctg gtcgtcaatt
     3361 tgatcggctt cgcgtccaac cagttcttcg tctggctgct ggtcaagaag ctgggcgggc
     3421 cgaactggtg gccggtgatc ccgatcattt tcgtgacccc cgtcttgacc ttcgcgctgc
     3481 accgccgatg ggtgtacaaa tgagcgtcgt gatcctttcg gtcatcgtcc cggtcaagga
     3541 cgagcaggac gcgatcgagc cgttcgtcgc ccgcgtgcgc gcggtcatcg aggcgctccc
     3601 cgaccccgcg tcgggcagct tcgagatcct gttcatcgac gatggcagca ccgatgcgac
     3661 catggagaaa atccgcgccg cccacgccgc cgacaaacgg gtccgggcgg tgtcgctgtc
     3721 gcgcaacttc ggcaaggagg cggcgctgtc ggccggcctc gaccatgcgc gcggcgacgc
     3781 gatcgtgccg atggatgccg acctccagga cccgcccgag ctgcttggcg agatgctcgc
     3841 gaaatggcgc gaggggttcg agatcgtcta cggcgtgcgc aacaaccgcg aatcggacag
     3901 cctgcccaag cggttgacgg ccgacctcta ttaccgggcg cacaactggc tgagcgcgga
     3961 caagatcccc gagcatgccg gggacttccg gctgctcgac cgcaaggtcg cggacgtgat
     4021 ccgccagatg cccgagcgga cccgcttcat gaaagggctg ttcgcctggg ccgggttccg
     4081 gcaggcatcg gtcagctaca cgcgcgagcc gcggcggatc ggcaagacca agttcgatta
     4141 ttggaagctg tggaccttcg cgctcgacgg gatcacctcg gcgtcgaccg ttccgctccg
     4201 cgtgtggagc taccttggcg gcatcgtcgc tctgttcgcg atcggctacg ctctgttcgt
     4261 ggtcgtccgc accctgatct ccggggtgca ggtgcccggc tatgcgtcgc tgatggtggc
     4321 cgtccttttc ctcggcgggc tgcagctgct gtcgctcggg atcctcggcg aatatgtcgg
     4381 gcggatcatg atcgagacca agaaccggcc gctttacgtg atccgcgagc gggtcggcgg
     4441 ggacgaataa atggaggccg ccgtcttcca gcggatggcg gagctggacg agagccactg
     4501 gtggttcgtc ggccggcggc ggatcctcga ccagctgatc cggcgcgagg tccggcctcc
     4561 gccgggcgcc cggatcctcg aggtcggctg cgggacgggg cacaatctcg agatgctcgg
     4621 ccgcttcgga acggtcgagg cgaccgagct caatgactcg gcgcgggcgc ttgcgagcga
     4681 gcgcctcggc cgtccggtga agcgcgcggc gctgcccgac ctgtcgatgc tcgccgacga
     4741 gcgttacgac ctggtcgcgc tgctcgacgt gctcgagcat gtcgccgacg accaagcggc
     4801 gttgcgggcg atccggtcga agctgaggcc gggcggcaag ctgctggtga cggtgccggg
     4861 caacccgtgg atgtggagcg cacacgatgt ctcgcaccac caccaccgcc gctaccgccg
     4921 gcgcgagctc gagcgcgtcg ccacggcggc ggggctcgaa atcgacctcc tgagcccgtt
     4981 caacagcctg ttgttcccgg cgatcgccgc ggcccggatc gccggccgcc tgctcgggcg
     5041 cgagagcgcc gacgacgcca tgccgccacc gccggtgaac cagttgctgc gagcgatctt
     5101 cgaaatggag gcggcgctta tcggccgggt gccgttcccg ttcggggtgt cgctggcggc
     5161 cgtcctgcgc cgcccggatt gacccggaac agctgcgagc cgggaccgct ccacacccgc
     5221 tgcatcccgg cgacccggcg gggatcgtaa ggcggcggct ggatcagcca cagatagtcg
     5281 aaatgctcgc gcggcagcgc cccgagcgcc cggtcgatcc tccagctgcg gggacggcag
     5341 ccgttcgggc ggacgatctg cgacgggtcg gccgaaaagg cgccggcgtc gcggttgcgc
     5401 agccgcagca ggttgaggcc gggagtcacc cactggtcgt tggaaaagcc gtggcggcgg
     5461 acgatcacca tcgccccaag atgggcgttg cgcgccatcg gccattcgcg tccgcacccc
     5521 cggccggtca gcgaggcgac acgggcgccc atcggcacat gctcgagcgc ggcgagcttc
     5581 gagcgctggt cgtcggcggc catcgccagg ctgacggtgt tcgccccgat ccggaccagg
     5641 aaaaatgcga gcccgaggac cgcgagcacc cttgccgtcg gcagatgcgt ctcgcggcgg
     5701 aagcggatcg ccaggatcgc gaccgcgaac acgaagggca gcagccgcat gtcggcgtag
     5761 gcggagctga agatcaccca tggcagaagc aggaagccgg cgagcaggac cagcgccgag
     5821 aaggccaggt tgcgcgacag ggtcagccgg cggctgaaga tcgcgtacag gatgaccagc
     5881 gcgacgaccg cgagcgacag ccagtcgaag gtcatccagc ggtcgcgaag gactccgagc
     5941 agggcctcga gcttcaggtt ccaggcgaac cattcgcggc tggcgggccc ctcgatgttg
     6001 ctgcgccagg ccaggatgac caacgccggg agcgccatga ccatcgcgtg caaagcggcc
     6061 cacatcccgg acctgcgcca gccggcgccg cggtcgtgct ggcgcaccgc ttcggccgaa
     6121 aagcacagca gccccaatgt cccccagccg aaggtgtggg cgaagaagac gatgaaggag
     6181 atcggcacga acagaagcgc gcgcaggcgg gtccggtcga gccggccgag ccgcagccac
     6241 aggccgaagg cgaggaacgc cagcgcc
//
LOCUS       DASTZO010000061         3709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_343536,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000061 DASTZO010000000
VERSION     DASTZO010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343536"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..2028)
                     /locus_tag="VFU20_01610"
     CDS             complement(148..2028)
                     /locus_tag="VFU20_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HEU5285189.1"
                     /translation="MATATHLLTEEEATAHAPLEAVVVRFAGDSGDGMQLTGGQFTLS
                     TALAGNDLATFPDFPAEIRAPQGTTFGVSAFQINFGSCAIETAGDQPDVLIAMNPAAL
                     KVNVGELREGGLIIADEGEFTQRNLAKAGYEANPLEDGSLAKWQLVHFNISQLTLDAV
                     KPFGLGNKEALRCKNMWTLGLALWMFDRDRQPIVDWLKAKFAKAPELAEANIAALNAG
                     HAYGETVEMGTPIRRHHIDPAPAEPGLYRTVTGAEALAVGLVAGAQLAGLKMFFGSYP
                     ITPASPLLHHLARLKEFGITTFQAEDEIAAICAALGASYAGQLGVTSSSGPGIALKTE
                     ALGLAVMTELPLVVVNSQRGGPSTGLPTKTEQSDLYQAVYGRNADSPLPVIAARSPAD
                     AFECAIEACRIAARYMTPVMLLTDGYIANAAEPWKVPDMAGYEPFPVQFFDRAPAEGE
                     AVLPYARNEDLARPWIKPGTPGLEHRIGGIEKAPGTGNIDYSPEAHAEMTRLRAAKVA
                     SVADTIPDQQVCLGEAGGELAVVGWGSTFGPIHQAVRRARQRGLDVSHIHVRHIVPMP
                     KNLGPLLKRFKRILVPEMNSGQLKTVLRDQFLVDAQSLSKVSGQPFRIAELEAAIERT
                     LA"
     gene            2156..2626
                     /locus_tag="VFU20_01615"
     CDS             2156..2626
                     /locus_tag="VFU20_01615"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HEU5285190.1"
                     /translation="MNDDHLYRRGVGVMLLNPERKVWVGQRLDNPTDAWQMPQGGLDD
                     GEEPWPGALRELEEETGIAARFVERVAECAGPLRYDLPDEMAGKLWGGRWKGQVQHWF
                     LARFTGTDADVNLATAEPEFSAWKWVDPAELPDLIVPFKRDLYRRLLAEFRAHL"
     gene            2676..>3709
                     /locus_tag="VFU20_01620"
     CDS             2676..>3709
                     /locus_tag="VFU20_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEU5285191.1"
                     /translation="MGRLTSTEQDCAERAAAEPMRGQVIIWAGINSGSRNLDGLAKMA
                     ELLANEFAALPGEVRLVEPAPVDAIDATGSTVQQQHGRNVHLKVRPDAPVQLLFTGHM
                     DTVFDVEHEFQYTRWLEEGVLGGPGVADMKGGIAVMLAALKAVEAGGAAANLGYEVVL
                     NSDEEVGSPGSAALIAEAARGKRAALTYEPAALPDGTLAGARPGSGNFVFVVRGRSAH
                     AGRNPEEGRNAVVAAADLALRLERGKGPRLSVNPARIDGGGPNNVVPDLAVLHVNLRP
                     ATPDDEARARALIESTVAMVGAEHDVAIEPHGGFGRPPKPMTPEAEDLFRLVQQAGAD
                     LGQAIGWQPSG"
BASE COUNT          564 a         1256 c         1312 g          577 t
ORIGIN      
        1 gggcaccagc ggacctcctg gtcgctcgcc cagtccttgg cggtggtgac gggggtgatc
       61 tcgttcacga cgcgccgccc gacagccaga cgatcaggaa cacgaccacg atcagcatca
      121 tcaccatcgg ccagacattg atcggtttca tgccaatgtc ctttctatcg ccgcctcgag
      181 ctcggcgatt cggaacggct ggccgctgac cttcgacagt gactgcgcgt cgacgaggaa
      241 ctggtcgcgc agaacggtct tcagctggcc gctgttcatc tcgggcacca ggatgcgctt
      301 gaagcgcttg agcagcggcc cgaggttttt gggcatcggg acgatgtggc ggacgtggat
      361 atggctgacg tcgagcccgc gctggcgggc gcggcgcacc gcctggtgga tcgggccgaa
      421 ggtcgagccc cagccgacca ccgccagctc gccgccggcc tcgccgaggc acacctgctg
      481 gtcggggatg gtgtcggcga ccgacgcgac cttggcggca cgcagccggg tcatctccgc
      541 atgggcctcc gggctgtagt cgatgttgcc ggtgcccggc gccttctcga tgccgccgat
      601 ccggtgctcg aggccaggag tcccgggctt gatccacggc cgagcgaggt cctcgttgcg
      661 cgcatagggg agcaccgcct cgccctccgc gggcgcccgg tcgaagaact ggaccgggaa
      721 cggctcgtag ccggccatgt ccggcacctt ccagggctcg gcggcattgg cgatatagcc
      781 gtcggtcaga agcatcaccg gggtcatgta gcgggccgcg atccggcacg cctcgatcgc
      841 acattcgaac gcgtcggcgg gcgagcgcgc ggcgatcacc ggcagcgggg aatcggcgtt
      901 gcggccgtag accgcctggt agaggtccga ctgttcggtc ttggtcggaa ggccggtcga
      961 cggcccgccg cgctgcgaat tgacgaccac cagcggcagc tcggtcatca ccgcgaggcc
     1021 cagcgcctcg gtcttgagcg cgatccccgg ccccgacgac gaggtgaccc cgagctggcc
     1081 cgcgtaggac gcgcccaagg ccgcgcagat cgccgcgatc tcgtcctcgg cctggaaggt
     1141 ggtgatcccg aactccttca ggcgcgccag atggtggagc agcggcgagg ccggggtgat
     1201 cgggtaggag ccgaagaaca tcttcagccc cgcgagctgg gctcccgcga ccagcccgac
     1261 cgccagcgcc tcggccccgg tgaccgtccg gtagaggccg ggctcggccg gcgccgggtc
     1321 gatgtggtgg cggcggatgg gcgtccccat ctcgaccgtc tcgccatagg cgtggccggc
     1381 gttcagcgcc gcgatattgg cctcggcaag ctccggcgcc ttggcgaact tggccttcag
     1441 ccagtcgacg atcggctggc ggtcgcggtc gaacatccac agcgccaagc ccagcgtcca
     1501 catgttcttg cagcgcagcg cttccttgtt gccgaggccg aacggcttca ccgcgtcgag
     1561 ggtcagctgc gagatgttga agtggaccaa ttgccatttg gcgaggctgc cgtcctcgag
     1621 cggattggcc tcatagcccg ccttggcgag gttgcgctgc gtgaactcgc cctcgtcggc
     1681 gatgatcagc ccgccttcgc gaagctcgcc cacgttcacc ttcagggccg cggggttcat
     1741 cgcgatcaga acgtcgggct ggtcgccggc ggtctcgatc gcgcagctgc cgaagttgat
     1801 ctggaacgcc gagacaccga acgtcgtgcc ctgcggagcg cggatttccg ccggaaaatc
     1861 gggaaaagtg gccaggtcgt tgccggccag agcggtcgac agagtgaact ggccgccggt
     1921 cagctgcatg ccgtcgccgc tgtcgcccgc gaagcgcacc accaccgctt ccagcggcgc
     1981 gtgcgcggtg gcttcttcct cggtcagcag atgggtggcg gtggccattg tgtcccgatt
     2041 caatcctttg cgcctcttta gaccccacat gccgtcattg cgagtgcagc gaagcaatcg
     2101 gggccgaacc caaagcggat tgcgtcgctg cttcgctgcc cgcaatgacg ggcggatgaa
     2161 cgacgaccat ctctaccgcc gcggagtcgg agtgatgctg ctcaacccgg agcgcaaggt
     2221 gtgggtcggc cagcgcctcg ataacccgac cgacgcctgg cagatgccgc agggcgggct
     2281 cgacgacggc gaggagccgt ggcccggcgc gctgcgcgag ctcgaggagg aaaccggcat
     2341 cgccgcgcgc ttcgtcgagc gcgtcgccga atgcgccggg ccgctgcgct acgacctgcc
     2401 ggacgagatg gcggggaagc tgtggggcgg ccggtggaag gggcaggtcc agcactggtt
     2461 cctcgcccgc ttcaccggca ccgacgccga cgtcaacctg gccaccgccg agcccgagtt
     2521 cagcgcctgg aaatgggtcg acccggccga gcttcccgac ctgatcgtgc cgttcaagcg
     2581 cgacctctac cggcggctgc tggccgagtt ccgcgcccat ttgtgacgcg tctgccggtg
     2641 tggcgttcgg ccggcggcgc ggctaggagg ccgccatggg gaggctcacg agcaccgagc
     2701 aggactgcgc cgagcgcgcc gcggccgagc cgatgcgcgg ccaagtcatt atctgggctg
     2761 gaatcaatag cgggtcgcgc aatctcgatg gattggcgaa aatggcagag ctgttggcca
     2821 atgagtttgc agcgttgccc ggggaagtgc ggctggtgga acccgctccc gtcgatgcga
     2881 tcgacgccac cggcagcaca gtgcagcaac agcatggccg caacgttcat ttgaaggtgc
     2941 ggcccgacgc gcccgtgcag ctgcttttca cgggtcacat ggacaccgtc ttcgacgtcg
     3001 agcatgagtt tcagtacacg cgctggctcg aagagggtgt cctgggcggg cccggggtcg
     3061 ccgacatgaa gggcggcatc gcggtgatgc tggcggcctt gaaagcggtc gaggccggcg
     3121 gcgccgcggc aaatcttggc tacgaggttg ttctcaacag cgacgaggag gtcggctcgc
     3181 cgggatccgc cgccctgatc gccgaggcgg cccgaggcaa gcgcgcggcg ctgacctatg
     3241 agcccgccgc tttgcccgac gggacgctcg ccggagcccg gccggggagc ggcaattttg
     3301 tcttcgtcgt ccgcggtcgc tccgcccacg ccgggcgcaa ccccgaggag gggcgcaacg
     3361 cggtcgtcgc cgctgccgac ctcgcccttc ggctcgagcg cggcaagggc ccgcggctgt
     3421 cggtcaaccc ggcgcgcatc gacggcggcg gaccgaacaa tgtcgtcccg gacttggccg
     3481 tgctgcacgt caatctgcgc ccggcgacgc ccgacgacga ggcccgcgcc cgggccctga
     3541 tcgaatcgac cgtcgccatg gtcggcgccg agcacgacgt cgcgatcgag ccccacggcg
     3601 gcttcggccg gccgcccaag ccgatgacgc ccgaggccga agacttgttc aggctggtcc
     3661 agcaggcggg cgccgacctc ggccaggcga tcggctggca gccgtcggg
//
LOCUS       DASTZO010000062         4406 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_395704,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000062 DASTZO010000000
VERSION     DASTZO010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4406)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4406)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4406
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395704"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2666)
                     /gene="alaS"
                     /locus_tag="VFU20_01625"
     CDS             complement(<1..2666)
                     /gene="alaS"
                     /locus_tag="VFU20_01625"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU5285192.1"
                     /translation="MTSTNDIRRSFLDYFEAAGHARVPSAPLVPHNDPTLMFVNAGMV
                     PFKNVFTGLETRPFATAASSQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KDRAIELAWALLTKEWGLPPEKLTVTVYHSDDEAFDLWKRIAGLPEDRLIRIATKDNF
                     WAMGADGPSGPCSEIFYDHGEHIPGGPPGSADEEGDRFVEIWNLVFMQFLQENDAIVG
                     DLPKPSIDTGMGLERIAAVLQGVHDNYDTDTFRALVTASEELTRTRADGERKASHRVI
                     ADHLRASGFLVADGVLPANEGRGYVLRRIMRRAMRHAHLLGAKEPLMHRLVPALVAEM
                     GAAYPELVRAQPLLEQVLSQEETRFRQTLVNGLKLLDEATASLPSGGTLPGETAFKLY
                     DTYGFPLDLTEQALVDKGLKVDTAGFDAAMAAQKAAARAAWKGSGERASDDVWFDLAE
                     EHGSTEFTGYSGDEAEGVVLAIVADGKAVQSAKAGDTVHILLNQTPFYGESGGQIGDT
                     GKLTSLNGFEGDVEDTSKPLGRLHALLTNVVKGSLTVGDTLHQIVDSERRDRVRANHS
                     ATHLLHAALRHRLGGHVTQKGSLVAPNRLRFDFSHPAALTPDDIAAIEAEVNAQIRSN
                     RPVTTRLMTPDEAIAAGAIALFGEKYGDEVRVLSMGGGLEDGASYSTELCGGTHVHAL
                     GDIQLFKIVSEGAVSSGIRRLEALTGEAARQWLTEREARLREAAALLKAAPDEVPARV
                     SALVEERKRLERELAEAKKALALGGSVGSGGGKSDAPAPEQVGGHAFLGQVVEGLDPK
                     GLRAAVDTMKQRVGSGIAALVAVNDGRASVAVGVTDDLASQVSAVELVKAAVAALGGQ
                     GGGGRPDMAQGGGPDGSKADEALSAVRGALEKV"
     gene            complement(2717..3361)
                     /locus_tag="VFU20_01630"
     CDS             complement(2717..3361)
                     /locus_tag="VFU20_01630"
                     /inference="COORDINATES: protein motif:HMM:NF018264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1109 domain-containing protein"
                     /protein_id="HEU5285193.1"
                     /translation="MATTDELIERLAADVGPTRRGAAARRLAGGVALGGAVALAAILA
                     WLGPPLQAVQSTGAAAFAMKLLFCVALLAAAFALLLLAGRPGHELGRRWLWLLVPPAL
                     VAVSAAMELSIAAPGLRGELWLGSTWQTCLLAITLLALPVFVGILWAFRRLAPTRLRP
                     AGLLAGLTAGSASATLYALYCPETSATFLASWYSLGILAAGLIGLAIGPRLLRW"
     gene            complement(3348..3896)
                     /locus_tag="VFU20_01635"
     CDS             complement(3348..3896)
                     /locus_tag="VFU20_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5285194.1"
                     /translation="MESVEAQLKTLMVAGLAGNGAAHRQLLTSAAVRLRGYFARRLGA
                     DCADLEDLVQETLVAIHRRRESYDPALPFTAWLHAIARYKLIDHLRRRGVRAEVSLEG
                     AAEMAAPDRADEYLAAAAVEQLLGGLPDKQRLAIRLTRLEGHSIAEAAAMTGQSPSGI
                     KVGIHRGIKRLMEQARGGHGND"
     gene            complement(4023..>4406)
                     /locus_tag="VFU20_01640"
     CDS             complement(4023..>4406)
                     /locus_tag="VFU20_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285195.1"
                     /translation="APPRMIAAIGMGEGVLPPPATFDAGIMAVAMAIHFALAIVLAII
                     LAWVISRFGLGTAASIVGGAAFGLAVYYINFYGFTALFPWFAMARGAISIVAHAVFGA
                     ILGAVYRLLHPRSGEDSAEAGERLA"
BASE COUNT          664 a         1574 c         1516 g          652 t
ORIGIN      
        1 accttctcca gggcgccgcg gaccgcgctc agcgcttcgt ccgccttgga gccgtcggga
       61 ccgccgccct gggccatgtc ggggcggccg ccgccgccct gcccgcccag cgccgcgacc
      121 gcggccttga ccagctcgac cgcgctgact tggcttgcga ggtcgtcggt gaccccgact
      181 gcgacgctgg cgcggccgtc gttgaccgcg accagggcgg cgatgcccga gccgacgcgc
      241 tgcttcatcg tgtcgaccgc ggcgcgcaag cccttgggat ccaagccttc gaccacctgg
      301 ccgaggaacg cgtggccgcc gacctgctcg ggcgccgggg cgtcggattt gccgccgcca
      361 ctgccgacgc ttcctcccag cgccagcgcc ttcttcgcct cggcgagctc gcgttcgagc
      421 cgcttgcgct cctcgaccag cgccgagacg cgcgccggaa cctcgtcggg cgccgccttg
      481 agcagcgccg cggcctcgcg cagccgcgcc tcgcgctcgg tcagccactg ccgcgcggcc
      541 tcgccggtca gggcttccag gcgccggata cccgacgaca ccgcgccttc gctgacgatc
      601 ttgaagagct ggatgtcgcc gagcgcgtgg acgtgggtgc cgccgcacag ctcggtgctg
      661 tagctcgctc cgtcttccag tccgccgccc atgctgagca ctcgcacctc gtcgccgtat
      721 ttctcgccga acaaggcgat ggcgccggcc gcgatcgcct cgtcgggggt catcagccgg
      781 gtggtcaccg gccggttgga gcggatctgc gcgttcacct cggcctcgat cgcggcgatg
      841 tcgtcgggcg tgagggccgc gggatgggaa aagtcgaagc gcagccggtt gggggcgacc
      901 aggctgccct tctgggtgac atggccgccg agccggtggc gcagcgcggc gtgcagcagg
      961 tgggtggcgc tatggttggc gcgcacccgg tcacggcgct cggaatcgac gatctggtgg
     1021 agcgtgtcgc cgaccgtgag gctgcccttg acgacgttgg tcagcagcgc gtggaggcgg
     1081 cccaaaggct tcgacgtgtc ctcaacgtcg ccttcgaagc cgttcaagga cgtcagcttt
     1141 ccggtgtcgc ctatctgtcc gccgctctcc ccgtagaagg gcgtctggtt gagcaggatg
     1201 tgcactgtgt cgccggcttt cgcgctttgc acggctttcc cgtccgcgac gatcgccagc
     1261 accacgccct cggcctcgtc gcccgagtaa ccggtgaact cggtcgaccc atgctcctcg
     1321 gcgaggtcga accagacgtc gtcggatgcg cgctcacccg agcccttcca ggcggcgcgg
     1381 gcggcggcct tctgcgccgc catggccgca tcgaagccgg cggtgtcgac cttgagcccc
     1441 ttgtcgacca gcgcctgctc ggtcaaatcg aggggaaagc cgtaggtgtc gtagagcttg
     1501 aacgcggttt cgcccggaag cgtgccgccc gacggaagcg acgcggtcgc ctcgtcgagc
     1561 agcttcaatc cgttgaccag ggtctggcgg aagcgcgtct cctcctggct cagcacctgc
     1621 tcgagcagcg gctgggcgcg gaccagctcg gggtaagcgg cgcccatctc ggcgaccagc
     1681 gccggcacca gccggtgcat cagcggctcc ttcgcgccga gcaggtgcgc gtggcgcatc
     1741 gcgcggcgca tgatccggcg gagcacgtag ccgcggccct cgttggccgg aagcacaccg
     1801 tcggcgacca ggaagcccga cgcgcgcaga tggtcggcga tcacccggtg gctggccttg
     1861 cgctcgccgt ccgcccgggt gcgggtcagc tcctcgctgg ccgtgaccag cgcccggaag
     1921 gtgtcggtgt cgtaattgtc gtggaccccc tgaagcaccg cggcgatgcg ctcgaggccc
     1981 atgccggtgt cgatcgacgg cttcggcagg tcgccgacga tcgcgtcatt ctcctgcagg
     2041 aactgcatga acaccaggtt ccagatctcg acgaagcggt cgccctcctc gtcggccgag
     2101 cccggcgggc cacccgggat atgctcgccg tggtcgtaga agatctccga acagggcccg
     2161 ctcggcccgt cggcgcccat cgcccagaaa ttgtccttgg tcgcgatgcg gatcagccgg
     2221 tcttcgggca ggcccgcgat ccgcttccac aggtcgaacg cctcgtcgtc gctgtggtag
     2281 acggtgaccg tcagcttctc gggcggaagc ccccactcct tggtcagcag cgcccaggcg
     2341 agttcgatcg cccggtcttt gaaatagtcg ccgaacgaga aattgccgag catctcgaag
     2401 aaagtgtggt ggcgcgcggt gtagccgaca ttgtcgaggt cgttgtgctt gcccccggcg
     2461 cgcacgcatt tctggctgct tgccgcggtg gcgaacgggc gggtctcgag gccggtgaac
     2521 acgttcttga acggcaccat cccggcgttg acgaacatca aagtcgggtc gttgtgcggg
     2581 accaagggcg ccgacggcac ccgcgcatgg ccggccgcct cgaaataatc gaggaagctt
     2641 cgccggatgt cgttggtcga ggtcatttga agccgatgta aatggcttgc tgcagtgcgg
     2701 caaggagccg acccccttac cagcgcagca gcctcggccc gatcgcgagg ccgatgagcc
     2761 cggcagcgag aatacccagc gaataccagc tggcgaggaa ggtcgccgac gtttccgggc
     2821 aatagagcgc gtagagggtc gccgaggccg atccggcggt cagtccggcg agcaggccgg
     2881 ccggccgcag ccgggtcggc gcgagccttc ggaacgccca caggataccg acgaaaaccg
     2941 gcagcgcgag caacgtgatc gccagcaggc aggtctgcca tgtagagccc agccacagct
     3001 ctccgcgaag cccgggcgcg gcgatcgaca gctccatcgc ggcgctgacc gcaaccaggg
     3061 ccggcggcac cagcagccac aaccagcgcc ggccgagctc atgacccggg cggccggcaa
     3121 gcaacagaag cgcgaacgcg gccgccagca gggcgacgca gaacagcaac ttcatcgcga
     3181 acgccgcggc cccggtggac tgcaccgcct gcaacggcgg ccccagccag gccaggatcg
     3241 ccgccaacgc aacggcgcca ccgagcgcga ccccaccggc cagccgccga gccgcagctc
     3301 cgcggcgagt cggcccgacg tccgcggcaa ggcgctcgat gagctcgtca gtcgttgcca
     3361 tggccgcctc tcgcttgctc catcaggcgc ttgattccgc ggtggatgcc gaccttgatc
     3421 cccgacggcg actggccggt catcgccgcc gcctcggcga tcgagtgccc ttcgaggcgg
     3481 gtcagccgga tcgccagccg ctgcttgtcg ggaaggccgc ccagcaattg ctcgaccgcg
     3541 gccgcggcga ggtactcgtc ggcgcggtcc ggagcggcca tttccgccgc tccttcaagc
     3601 gatacctcgg cgcggacgcc gcgccggcgc aaatgatcga tcaatttgta gcgggcgatc
     3661 gcgtgcagcc aggcggtgaa cggaagcgcc gggtcgtagc tctcgcgcct gcggtggatg
     3721 gcgaccagcg tttcctgcac cagatcctcc aggtccgcgc agtcggcccc cagccgccgc
     3781 gcgaaatagc ccctcaggcg gacggcggcg gacgtcagca gctggcggtg cgcggccccg
     3841 ttgcccgcca ggccggcgac catcagcgtc ttcagctggg cttccaccga ctccatggcc
     3901 gggtgcgacg ctcccgttcg gcaggggcgc ggccgcggct gggccgggct ccttcagttc
     3961 gggagcaccg tagagcaacc tggtggccat tgcgaaccgg cggatgcccg cctgcccatg
     4021 agtcaggcga gccgttcgcc ggcctcggcc gaatcctcgc cggacctcgg gtggagcagc
     4081 cggtagacgg cgccgaggat cgccccgaac acggcgtgag cgacgatgct gatcgcgccc
     4141 cgggccatcg cgaaccacgg gaacagagcc gtgaagccgt agaaattgat gtaatagacg
     4201 gcgagcccga acgccgcccc gccgacgatc gacgccgcgg ttcccagccc gaaacgcgaa
     4261 atgacccagg cgaggatgat cgccagcacg atcgccagcg cgaaatggat cgccatcgcc
     4321 accgccatga tcccggcgtc gaaggtcgcc ggcggcggga gcacgccctc gcccatgccg
     4381 atcgcggcga tcatccgcgg cggcgc
//
LOCUS       DASTZO010000063         3995 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_501255,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000063 DASTZO010000000
VERSION     DASTZO010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3995)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3995)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3995
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501255"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="VFU20_01645"
     CDS             <1..1543
                     /locus_tag="VFU20_01645"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEU5285196.1"
                     /translation="GSLSWEQPQALAAFTRESPFFDLPVPNDVSVSRQVLAEPDATLT
                     ERTWATLADGTPLVTAQRRGKGLIVLFHVTADTRWSDLPLSGTFVEMLKRIVSLSGSI
                     AAGDTAASGSRATREVLPPTRILDGFGIFGPPPGTARPVPVGFGGRATFDHPPGFYGP
                     PEGLLAINTLAPADRLAPLDLSGLNARREAYRMSEPQDLRGPILITALALLALDALIV
                     FLMAGGLRRFSRPGRPAAAAVALALLAAGLMASPQTFAQDSKEQQALKSTLETRLAYV
                     ITGDAEVDRVSKAGLQGLTLFLAQRTALEAGEPVGIDPGRDELSFYPLIYWPVVPSAP
                     KPSREALEFIDAYMKRGGTVLFDTRDAVEAPPGPGGATQGPGMVALRSILGALDIPEL
                     EPVPRDHVLTKTFYLLKEFPGRFASGQLWVEATPRNEDEEADTRPARAGDGVSSLIIT
                     SNDLAGAWAMRPDGQPMLPMMSSEPRQREFAFRAGVNIVMYTLTGNYKADQVHIPALL
                     ERLGQ"
     gene            1679..3745
                     /locus_tag="VFU20_01650"
     CDS             1679..3745
                     /locus_tag="VFU20_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285197.1"
                     /translation="MNFGISFAPLVPAAVLWVAAAIAFLIAMLLVFARSRGALMRSIA
                     LALFLLALANPSITREDRDPLTSVAVVVIDKSASQEFADRTDQTEAARAQVAERLGRI
                     PNLEVRFVEAGQSDGETDGTRLFTALTSALADVPQDRIAGAIMITDGRIHDLPTDFAG
                     LGFSAPVHGLITGHANERDRRVVLVTTPRFGIVGQSQAITFKIEDQGVSPGTAQVTIR
                     RDGEQVDSRSVRVGSNVTVNVQIAHAGPNIVEIEAAPIDDELTEVNNRAVVSIDGVRD
                     KLRVLLVSGEPHAGERTWRNLLKSDASVDLVHFTILRPPEKQDGTPINELSLIAFPTR
                     ELFQQRINDFQLIIFDRYARQGVLPIIYFDNIARYVRNGGAILVAAGPDYASQTSLWR
                     TPLEVVLPAEPNGRTTDGAFRAALTELGKRHPVTRGLEGSESDPPHWSQWFRLVDSRT
                     RKGTAVMHGPDNKPLLVLSREGEGRVALLLSDHIWLWARGYEGGGPHIDLLRRLSHWL
                     MKQPDLEEEALRMFVRGRDLTVQRQTMSEEVSEITLTTPSGKSRNLTLTQAEPGVWRS
                     TLEASELGLWRATDGTLTALANVGPANPREFTEVTSTRQVLAPLAAATGGDVVRIEDG
                     RGISLPRIVATRSSDTFHGESWIGLRIREASVVRGIGVLPVFAGLIGLLLLVGALAAT
                     WAREGR"
BASE COUNT          621 a         1420 c         1337 g          617 t
ORIGIN      
        1 cggatcgctg tcgtgggagc agccgcaggc gcttgccgcc ttcacgcggg agagcccgtt
       61 cttcgacctg ccggtgccga acgacgtcag cgtaagccgc caggtgctgg cggagccgga
      121 cgccacgctg accgagcgca cctgggcgac gctggcggac ggtacaccgc tcgtcaccgc
      181 gcagcgccgc ggcaagggcc tgatcgtgct gttccacgtc accgccgata cacgctggtc
      241 tgatctgccg ctttcgggca catttgtcga aatgctcaag cgaatcgttt ccctgtcggg
      301 ctcgatcgct gccggcgaca ccgctgccag cggcagccgc gctacccgcg aggtgctgcc
      361 accgacgcgg atactcgatg gttttggcat tttcggcccg ccgccgggca ccgcacggcc
      421 ggtgccggtc ggcttcggcg gccgtgcaac cttcgaccac ccgccggggt tctatggtcc
      481 gccggaagga ttgctggcga tcaacacgct tgcacccgct gatcgcctcg cgccactcga
      541 cctgtcgggg ctcaacgcac gccgggaggc ttatcgaatg agcgagccac aagacctccg
      601 cggcccgatc ctgatcaccg cacttgcgct gctcgcgctc gacgcgctga tcgtgttcct
      661 gatggcgggc ggcttgcggc ggttctctcg ccccggccgg ccggccgccg ccgcggtggc
      721 gctggcgctc ctcgccgcgg gcctgatggc atctccacag accttcgcgc aggactccaa
      781 agagcagcag gcgctgaagt cgacgctgga gacgcggctc gcctatgtga tcaccggcga
      841 cgccgaggtc gatcgcgtca gcaaggcggg cctgcagggc ctgacgctgt tcctggcaca
      901 gcgcaccgcg ctcgaagccg gcgagccggt cgggatcgat ccggggcgcg acgagctgtc
      961 gttctatccg ctgatctact ggcccgttgt gccgagcgcg ccgaagccct cgcgcgaggc
     1021 gctggagttc atcgacgcct acatgaagcg cggcggcacc gtgctgttcg atacccgcga
     1081 cgccgtcgag gcgccgcccg gcccgggcgg agccacgcaa ggacccggca tggtcgcgct
     1141 gcgctcgatc ctgggcgcgc tcgacattcc cgagctcgag ccggtgccgc gcgaccacgt
     1201 gctgaccaag acgttctacc tgctcaagga gtttccgggc cgcttcgcct ccggccagct
     1261 ctgggtcgag gcgacgccgc gcaacgagga cgaggaagcc gacacccgcc cggcgcgggc
     1321 cggcgacggc gtgtcctcac tgatcatcac gtcgaacgac ctcgccgggg cctgggcgat
     1381 gcggccggat ggccagccga tgctgccgat gatgtcctcg gaaccgcgcc agcgcgaatt
     1441 cgcgttccgc gccggcgtca acatcgtgat gtacacgctg accggcaact acaaggccga
     1501 ccaggttcat atcccggcgc tgctggagcg gctcggacag taacgttgcg gcctcatcct
     1561 tcgagacgcg cacttcgtgc gctcctcagg atgaggaaaa ggtgctggtc ctcatggtga
     1621 ggagcgtcgc gcagcgacgc gtctcgaacc atgcggcccc gatagtgagg taatggagtt
     1681 gaacttcggg atttcgttcg cgccactggt tccggctgcc gtcctctggg tggcggcggc
     1741 gatcgcgttc ctgatcgcaa tgctgctggt gttcgcgcgc agccgcggcg cgctgatgcg
     1801 ctcgatcgcg ctggcgctgt tcctgctggc gctggccaat ccgtcgatca cccgcgagga
     1861 ccgcgatccg ctgacctcgg tcgcggtcgt ggtgatcgac aagagcgcga gccaggaatt
     1921 cgccgaccgc actgaccaga ccgaagcggc ccgcgcccag gtcgccgagc ggctcggccg
     1981 catccccaat ctcgaagtgc ggttcgtcga agccggccag tccgacggcg agaccgacgg
     2041 cacgcggctg ttcacggcgc tcacttccgc gcttgccgac gtgccgcagg accgcatcgc
     2101 cggcgccatc atgatcaccg acggccgcat ccacgacctg ccgaccgact tcgccggact
     2161 gggcttctcc gcgccggtgc acgggctgat caccggccac gccaacgagc gcgaccgccg
     2221 ggtcgtgctg gtcaccacac cgcggttcgg catcgtcggt cagagccagg cgatcacctt
     2281 caagatcgag gaccagggcg tctcgcccgg caccgcgcag gtcaccatcc gccgcgacgg
     2341 cgagcaggtc gacagccgca gcgtccgggt cggcagcaac gtcaccgtca acgtgcagat
     2401 cgcccatgcc ggtccgaaca tcgtcgagat cgaggccgcg ccgatcgacg acgagctgac
     2461 cgaagtcaac aaccgcgcgg tggtctcgat cgacggcgtg cgcgacaagc tgcgcgtgct
     2521 gctggtttcg ggcgagccgc atgccggcga gcgcacttgg cgcaacctgc tcaagtcaga
     2581 cgcttcggtc gacctcgtcc acttcaccat cctgcgcccg ccggagaagc aggacggcac
     2641 gccgatcaac gagctatcgc tgatcgcgtt cccgacccgc gagttgttcc agcagaggat
     2701 caacgacttc cagctcatca tcttcgaccg ctacgcccgc cagggcgtgc tgccgatcat
     2761 ctatttcgac aacatcgccc gctacgtccg caacggcggc gccatcctgg tcgcggccgg
     2821 tcccgactac gcgagccaga ccagcctctg gcgcacgccg ctggaagtcg tcctgccggc
     2881 ggagccgaac ggccgcacca ccgatggcgc gttccgggcg gctctcaccg aactcggcaa
     2941 gcgtcatccg gtgacgcgcg ggctcgaagg ctcggagagc gatccgccgc actggagtca
     3001 gtggttccgg ctggtcgaca gccgcacccg caagggcacg gcggtgatgc atggccccga
     3061 caacaagccg ttgctggtgc tgtcgcgcga aggcgaaggc cgcgtcgcgc tccttctctc
     3121 cgaccacatc tggctgtggg cgcgcggcta cgaaggcggc ggcccgcaca tcgacctgct
     3181 gcggcggctg tcgcactggc tgatgaagca gcccgacctg gaggaagaag cgctgcgcat
     3241 gttcgtgcgc ggccgcgacc tcacggtgca gcgccagacc atgtcggaag aggtcagcga
     3301 gatcacgctg acgacgccgt ccggcaagag ccgcaacctc acgctgacgc aggccgagcc
     3361 cggcgtctgg cgctcgacgc tcgaggccag cgagctcggg ctgtggcgcg ccaccgacgg
     3421 cacgctgacc gcgctcgcca acgtcggccc ggccaatccg cgcgaattca ccgaggtgac
     3481 ctcgacccgg caggtgctgg cgccgctcgc ggcagcgacc ggcggcgacg tcgtgcgcat
     3541 cgaggatggc cgcggcatca gcctgccgcg catcgtcgcg acgcgctcgt ccgacacttt
     3601 ccacggcgag agctggatcg ggctgcgcat ccgcgaggcg agcgtggtcc gcgggatcgg
     3661 cgtgctgccg gtgttcgccg ggctgatcgg cctccttctg ctggtcggcg cgctcgccgc
     3721 cacgtgggcc cgcgaaggcc gctgacgcgg cagaactccg cctctcgtca ttccgggacg
     3781 gcccgaaggg ccgggcccgg aatccatcca tcgatttgaa ccaccgcgag atggattcgg
     3841 ggctcgcgct gacgcgcgcc ccggaatggc ggggtcaaac ggtgttgaat tcaagcaaca
     3901 gcggcggcgc aatcgccctg accatattgt tacattgtaa cactacggct atgaatccgt
     3961 ccgggcggca ggcaatgtct gccgggggat tcgac
//
LOCUS       DASTZO010000064         3623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_684084,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000064 DASTZO010000000
VERSION     DASTZO010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3623
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684084"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..652)
                     /locus_tag="VFU20_01655"
     CDS             complement(125..652)
                     /locus_tag="VFU20_01655"
                     /inference="COORDINATES: protein motif:HMM:NF025710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HEU5285198.1"
                     /translation="MSRFLLLAPVLALAAGMSAVPAMPAAAQCRLCETPTTGRGDGPA
                     DGPVSLEIETSLDFDRLILLGSGEGSATLLPDGSRSVSGVVGAISGRAMVGSAVVRGE
                     AGRAVRIELPRRIELYSGSGARIAIEDIVSDLPANARLDSAGALAFRFGGRLRISGDA
                     EGDYRGDVPITVEYL"
     gene            795..2897
                     /locus_tag="VFU20_01660"
     CDS             795..2897
                     /locus_tag="VFU20_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU5285199.1"
                     /translation="MPPIPSAPRRPFVTALKVVLYTGLVGLLALIVAVAVAVASLPGY
                     SELTRRSDLGQMIRVRSSNGTVLVSLGPSFGKWLRYEQIPPQMRSAMIAIEDKRFRSH
                     IGVDPLGVARSIKVRLDTGAWRQGGSTITQQLARNIFLTNSRTFGRKVKEGVLALALE
                     RKFSKDQILELYLNRVYFGGGAYGIDAASRRFFGHSADTLSLGEAAIIAGLVKAPSNY
                     SPTADAEAARRRSSIVLDAMVENGFVSPSAAASVDPAEIRLQPTTKQNSVRYFTDWAL
                     PQLDTLIDETSEPIDVWTTLDTSMQRAADRAIAANTPRTAQGALVALDRDGAVRAMVG
                     GRDYVDSLYNRATQAERQPGSAFKLFVYLAALESGMKPTDTIVDEPIEIDGWRPRNAT
                     RTHQGPVTLREAFARSINTISAKIGAELGFATIADMARRFGISGKISTFPSMVLGTSD
                     VRLIDMTRAFASVANRGVSVAPYAIRRVVTADGRQLFQHESSEERVLVAPWVAAEMTD
                     LLQSAVLTGTGRAAQIGRPVAGKTGTTSSNKDGWFIGFSSGITAGVWMGRDDARPVGG
                     LQGGTAPARAFHDFMVVAVARRPVEQFETEVPMPDWQLEPEEEIWGLPPEELGLEPIV
                     DADGNPIVPGDPDVAQPGVVAPDGAAPPPGWPEEILRSGERPPQPQPVAPPQPQRAPP
                     PRSAPAPDPLQPRPDPPQ"
     gene            complement(2847..>3623)
                     /locus_tag="VFU20_01665"
     CDS             complement(2847..>3623)
                     /locus_tag="VFU20_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjP-like metallopeptidase domain-containing
                     protein"
                     /protein_id="HEU5285200.1"
                     /translation="AAGRSARTMRGTLNFMSSSGRSEQLAVHPGLPVPVEIRAVRGAR
                     RLRLRLDERRGVLKLTCPMRTSRRAALAWAAEQREWVDTQLADMLPAEPFAAGERIPV
                     EGLETRLVWTAGEARVPRLDSGELRCGGLESGFERRIETFLKRLALETLSSETAEAAA
                     AAGVAPRSVSVGDANTRWGSCSSERRIRYSWRLILAPPAARRYVVAHEVAHLVHLDHG
                     PGFKALERRLFAGFASGEVAAARSLLRRVGPRLKRIGRGR"
BASE COUNT          542 a         1340 c         1254 g          487 t
ORIGIN      
        1 tcacccgtgg tcccgtcatt aacggcgcgc gctttcggtt ttcctcaagg gattggttaa
       61 cggccggttc gtacttggtc cgaaacgaac ctggttttcc caagggcgat tcagaggcgg
      121 cggctcagag atattcgacg gtgatcggaa cgtcgccgcg atagtcgccc tcggcgtcgc
      181 cggagatccg cagccggccg ccgaatcgaa aagccagggc gccggccgaa tcgagccggg
      241 cgttggcggg caagtcgctg acaatgtcct cgatggcgat tcgcgcgccg ctgcctgagt
      301 aaagctcaat ccgccgcggc agctcgatcc gaaccgcccg gccggcctcg ccgcggacga
      361 ccgcgctgcc gaccatcgcc cggccgctga tcgccccgac gaccccgctg acgctgcgcg
      421 agccgtcggg aagcagggtc gcactgcctt cgcccgagcc cagcaggatc agccggtcga
      481 aatcgaggct ggtctcgatc tcgaggctga ccgggccgtc ggccggcccg tcgccgcggc
      541 cggtggtcgg agtctcgcac agccggcatt gcgccgccgc cggcatcgcc ggcacggccg
      601 acatccccgc ggcgagcgcg aggaccggcg ccaggagcaa aaacctgctc atcgccgcga
      661 tcctaacggc gactggctaa agcagggtta atccgccggt aacccggccg gcgacaaggg
      721 aacggaagcc gccggccggc gattgtcgcg cgcctattgt ccccaagggc gcgactttct
      781 atcgctgggc gctcatgccc cccatcccct ccgctccgcg ccggccgttc gtcaccgcac
      841 tgaaggtcgt gctctacacc ggcctggtcg ggcttctggc gctgatcgtc gcggtcgcgg
      901 tcgcggtcgc gtcgctgccc ggctattccg agctgacccg gcgctccgac ctcggccaga
      961 tgatccgggt ccggtcgagc aacggcacgg tgctggtgtc gctcggcccg agcttcggca
     1021 agtggctgcg ctacgagcag atcccgccgc agatgcgcag cgcgatgatc gccatcgagg
     1081 acaagaggtt ccgcagccac atcggcgtcg acccgctggg ggtcgcccgg tcgatcaagg
     1141 tgcggctcga caccggcgcc tggcgccagg gcggctcgac catcacccag cagctcgccc
     1201 gcaacatctt cctgaccaac agccggacct tcgggcgcaa ggtcaaggaa ggggtgctgg
     1261 cgctggcgct ggagcgcaag ttcagcaagg accagatcct cgagctctat ttgaaccggg
     1321 tctatttcgg cggcggcgcc tacggcatcg acgccgcctc gaggcgcttc ttcggccaca
     1381 gcgccgacac attgtcgctc ggcgaagcgg cgatcatcgc cgggctggtc aaggcgccgt
     1441 ccaactattc gccgaccgcc gatgccgagg ccgcccgccg ccggtcgagc atcgtcctcg
     1501 atgcgatggt cgagaacggc ttcgtcagcc cgtcggccgc ggcgtcggtc gacccggcgg
     1561 aaatccggct gcagccgacc accaagcaga acagcgtccg ctatttcacc gactgggcgc
     1621 tgccgcagct cgacacgttg atcgacgaga ccagcgagcc gatcgacgtc tggacgacgc
     1681 tcgacaccag catgcagcgc gccgccgacc gggcgatcgc cgccaacact ccgcgcaccg
     1741 cccagggggc gctggtcgcg ctcgaccgcg acggggcggt gcgggcgatg gtcggcggcc
     1801 gcgactatgt cgattcgctc tacaaccggg cgacccaggc cgagcggcag ccgggatcgg
     1861 cgttcaagct gttcgtctat ctggcggcgc tcgaatcggg gatgaagccg accgacacca
     1921 tcgtcgacga gccgatcgag atcgacggct ggcggccgcg caacgcgacc cgcacccacc
     1981 aggggccggt caccctgcgc gaggcgttcg cccgctcgat caacacgatc agcgccaaga
     2041 tcggcgccga gctcggcttc gcgaccatcg ccgacatggc ccgccgcttc ggcatcagcg
     2101 gcaagatctc gacctttccg tcgatggtgc tggggaccag cgacgtccgg ctgatcgaca
     2161 tgacccgcgc cttcgcctcg gtcgccaacc gcggcgtctc ggtcgcccct tacgcgatcc
     2221 gccgggtggt caccgccgac ggccgccagc tgttccagca cgagtcgagc gaggagcggg
     2281 tgctggtcgc cccgtgggtg gcggcggaaa tgaccgacct cttgcagtcg gcggtgctga
     2341 ccgggaccgg ccgggcggcg cagatcggcc ggccggtcgc cggcaagacc ggcaccacca
     2401 gctccaacaa ggacggctgg ttcatcggtt tctcgagcgg catcaccgcc ggcgtgtgga
     2461 tgggccgcga cgacgcccgg ccggtcggcg ggctgcaggg cggcaccgcg ccggcgcgcg
     2521 cgttccacga cttcatggtc gtcgccgtcg cccggcgccc ggtcgagcag ttcgagaccg
     2581 aggtgccgat gcccgactgg cagctcgagc ccgaggagga aatctggggc ctcccgcccg
     2641 aggagctcgg cctcgagccg atcgtcgacg ccgacggcaa cccgatcgtc ccgggcgacc
     2701 cggacgtcgc ccaacccggg gtcgtggccc ccgacggcgc cgcgccgccg cccggttggc
     2761 ccgaggagat cctcaggagc ggcgagcggc cgccgcagcc gcagccggtg gcgccgccgc
     2821 agccgcaacg agcgccgccg ccgcgctcag cgcccgcgcc cgatccgctt cagccgcggc
     2881 ccgacccgcc gcagtagcga ccgggcggca gcgacctcgc cgctggcaaa gccggcgaac
     2941 aggcgccgct cgagcgcctt gaacccgggc ccgtggtcga ggtggaccaa atgcgcgacc
     3001 tcgtgcgcga cgacgtagcg gcgggccgcg ggcggggcga ggatcagccg ccagctgtag
     3061 cggatccgcc gctcggacga gcagctgccc cagcgcgtgt tggcgtcgcc gacgctgacc
     3121 gagcgcggcg ccactccggc cgcggccgcg gcctcggcgg tctcgctcga cagggtctcg
     3181 agcgcgaggc gcttgaggaa ggtctcgatt cggcgctcga agccgctctc gaggccgccg
     3241 cagcgcagct cgcccgaatc gaggcgcggg acgcgagcct cgcccgcggt ccacaccagc
     3301 cgagtctcga gcccttcgac cgggatccgc tcgccggcgg caaagggctc ggccggaagc
     3361 atgtcggcaa gctgggtgtc gacccactcg cgctgctcgg ccgcccaggc cagcgccgcc
     3421 cggcggctgg tgcgcatcgg gcaggtcagc ttgagcactc cgcggcgctc gtcgagccgc
     3481 agccggagcc ggcgggcgcc gcgcaccgct cggatctcga ccgggaccgg cagtccggga
     3541 tggacagcta gctgctcaga gcgcccgctc gacgacatga aattcaaggt cccccgcatc
     3601 gtcctcgctg accgtccagc cgc
//
LOCUS       DASTZO010000065         6017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_369632,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000065 DASTZO010000000
VERSION     DASTZO010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6017
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_369632"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..877
                     /locus_tag="VFU20_01670"
     CDS             <1..877
                     /locus_tag="VFU20_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5285201.1"
                     /translation="WDPFIPPPTGEKGEFGWSGILRGASIVFFAYIGFEAVSTAAQEA
                     KNPAKDMPFGVIGSLVACTIIYILVSVVLTLIVPYTELNVPDPVAVAVDAFGPQWGWF
                     ASTIKVGAIIGLTSVILVLMYGQTRIFYTMARDGLLPKIFSRVHPKFRTPWINTLVVG
                     LLVAIAAGFFDINTLGDMTSVGTLAAFGIVCLTVIWLRQTHPDIPRGFRVPFYPVFPA
                     LGVLSSFALIFSVETRVLIFFAWFVVGAIVLYFAYGMRNSELAKGHPVMADDETPYFP
                     EDAPLGPDGKPVIRP"
     gene            complement(915..1910)
                     /locus_tag="VFU20_01675"
     CDS             complement(915..1910)
                     /locus_tag="VFU20_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEU5285202.1"
                     /translation="MPSPRLDVLAIGNAIVDVIADADDAFLDAEGLTKGSMRLIDAEE
                     AERLYAHMGPAREVSGGSAGNTAAGVAALGGRAGFIGQVAPDQLGQFYIHDLRAAGVE
                     FTTPPADFGVPTARSMVLVTPDGHRTMNTFLGAAQHLPASALDEAQIEGSAMLYLEGY
                     LWDPETPRYAMVRAIEVARNAKRKVAFTLSDSFCIERHRDGFNALIDDRRIDILFANE
                     VEIQAMAGLPHLETAIAAYCDKVETLVVTRSEKGALATRGGERAEVPAEPVKEVVDTT
                     GAGDLFAAGFLVGEARGLGLEKSLRLGAIAAADVIQHYGARPEADLRALAGDLLG"
     gene            1983..2339
                     /locus_tag="VFU20_01680"
     CDS             1983..2339
                     /locus_tag="VFU20_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285203.1"
                     /translation="MRRAVAFALLLVAGCATAPQPQPEQPRPLPATPAPQLRGELIGL
                     TASELVQRLGNPALQIREGQSLKLQFRGSRCILDAYLYPPRDSSGPERVAHVDARLRS
                     GAETDPRVCVATLSRT"
     gene            complement(2336..3376)
                     /gene="queG"
                     /locus_tag="VFU20_01685"
     CDS             complement(2336..3376)
                     /gene="queG"
                     /locus_tag="VFU20_01685"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEU5285204.1"
                     /translation="MDRSQRLKDAIRAEAERLGFVACGFARADAAADSGLDLGRWLAA
                     GHHGTMGWMEERADQRASPLALWPDARSAIALGMSYAPATDPRALAAEPAIGRISAYA
                     QGGDYHKTVKKALKALARFIVERAPSELKVFVDTAPVMEKPLSAAAGIGWQGKHTNLV
                     SREHGSWLFLGIILTSLELEPDEPASHHCGSCTRCLDVCPTQAFDAAHRIDARRCISY
                     LTIEHSGPIPNEFRRAMGNRIYGCDECLAVCPWNRFAAAAAANRAFLPRAELAAPRLG
                     DLLALDDAAFREMFAGSPIKRIGRARMVRNCLIAAGNSGDPGLVKDVEPHLADSDPVV
                     AEAAAWAMEQLA"
     gene            3430..4383
                     /locus_tag="VFU20_01690"
     CDS             3430..4383
                     /locus_tag="VFU20_01690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5285205.1"
                     /translation="MAQHEPMTEDPAVEAVDLVKDFGETRALDGISLAVPAGSIYGLL
                     GPNGAGKTTALRVMLGIIDPSSGTRRVLGREQPLDAAWEVGYLPEERGLYSGMTAREA
                     IAFMGALRGLALKEGRRRADELLAAHGLGEWARKPIRNLSKGMAQTVQLLGTIVHRPR
                     LLVLDEPFAGLDAINQARLEELIRGRARDGVTVIFSTHVIAHAERLCERIAIIAGGKI
                     AFEGAVDEARDRLRPIVRLRTRSAEGPWRSAIPANARRERGEWVFELPPGGPEPLLKA
                     LIDGGAGIETLAIERPGLHDAFVAIAGDAAAAAMGDLGEPG"
     gene            4383..5591
                     /locus_tag="VFU20_01695"
     CDS             4383..5591
                     /locus_tag="VFU20_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5285206.1"
                     /translation="MSLLRSAFVIARRDFTATIFSRAFFFFLLGPLFPVLMMVIFGSI
                     GGAAVSEIERPVVAVVASDADFQALAEARRRLDEAVDPPAMITLRHVPPEPDSAAQRA
                     RLLAAQSPPVIAVLEGGIDRPRATGALLPDGTTVRQLRFLIEDARRAQTAPPIAEANV
                     LLTRTQRSPGLLAPARAMTARMGQMVLFLLSLLLSGMLLSQLIDEKANKIIEVLAAAV
                     PIDAIFLGKLSAMLAMSLLGLAVWTSLGAAAIAVMTGQSLSALPSPAVGWPAFLTLAV
                     VYFMMSYLLLGSVFLGIGGQASTAREVQTLSMPVTMAQVVVFAIAASAVGAPNSARAL
                     AAVVFPLSSPFVMIARAAELPQIWPHLLAIAWQSLWVALILHFASRVFRRIVLKSGPS
                     RPFWRRAAKA"
     gene            complement(5588..>6017)
                     /locus_tag="VFU20_01700"
     CDS             complement(5588..>6017)
                     /locus_tag="VFU20_01700"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU5285207.1"
                     /translation="AVGCNFEDQVIGGEGVHPLELQVRRIEAIQRAVGDDFFINARTD
                     LYLKTQQHDSALVDAAIERGKAFSDAGASGFFVPRLADPRQIERIVRDVPLPLNVIAF
                     PGAPDAQAVAQAGVARISHGPFPHRALMAKLEEMARAALN"
BASE COUNT          803 a         2144 c         2106 g          964 t
ORIGIN      
        1 ctgggatccg ttcatcccgc cgccgaccgg cgagaaaggc gagttcggct ggagcggaat
       61 cctccgggga gcgtcgatcg tcttcttcgc ctacatcggt ttcgaagcgg tctcgaccgc
      121 cgcccaggaa gcgaagaacc cggccaagga catgccattc ggggtgatcg gctcgctggt
      181 cgcctgcacg atcatctaca tattggtgtc ggtggtcctg accctgatcg tcccctacac
      241 cgagctcaac gtgcccgatc cggtcgcggt cgcggtggat gcgttcgggc cgcaatgggg
      301 gtggttcgct tcgaccatca aggtcggcgc gatcatcggc ctgacctcgg tgatcctggt
      361 gctgatgtac ggccagacgc gcattttcta caccatggcg cgcgacgggc tgctgccgaa
      421 gatcttctcc cgggtccatc ccaagttccg caccccgtgg atcaacacgc tggtggtcgg
      481 gctgctggtc gcgatcgccg cgggtttctt cgacatcaac acgctcggcg acatgacctc
      541 ggtcggcacc ctcgcggcct tcgggatcgt ctgcctgacg gtgatctggc tgcgccagac
      601 ccacccggac atcccgcgcg gcttccgggt gcccttctat ccggtgttcc cggcgctcgg
      661 cgtcctctcc tcgttcgcgc tgatcttctc ggtggaaacg cgggtgctga tcttcttcgc
      721 ctggttcgtg gtcggagcga tcgtcctcta cttcgcctac gggatgcgca attcggagct
      781 cgccaagggc catccggtga tggccgacga cgagactccc tacttcccgg aggacgcgcc
      841 gctcgggccg gacggcaagc cggtcatccg gccgtaagct ggaattccct ccccggcatc
      901 ggccggggag ggccctagcc cagcaggtcg ccggcgagcg ccctcaagtc cgcctcgggg
      961 cgcgcgccgt aatgctggat cacgtcggcc gcggcgatcg cccccagcct cagcgacttc
     1021 tcgagcccca gcccgcgcgc ctcgccgacc aggaagcccg ccgcgaacag gtcgcccgcg
     1081 ccggtcgtgt cgacgacctc cttcaccggc tcggccggga cctcggcccg ttccccgccg
     1141 cgggtcgcca gcgcgccttt ctcgctgcgg gtgacgacca gcgtctcgac cttgtcgcaa
     1201 taggcggcga tcgcggtctc gaggtggggc agcccggcca ttgcctggat ctccacctcg
     1261 ttggcgaaaa ggatgtcgat ccgccggtcg tcgatcaggg cgttgaagcc gtcgcggtgg
     1321 cgctcgatgc agaagctgtc ggacagagtg aaggcgacct tgcgcttggc gttgcgggcg
     1381 acctcgatcg cccgcaccat cgcgtagcgc ggagtctccg ggtcccacag atagccctcg
     1441 aggtagagca tcgccgagcc ctcgatctgc gcctcgtcga gcgcgctggc cggcagatgc
     1501 tgcgcggcgc cgaggaaggt gttcatcgtc cggtggccgt cgggagtcac cagcaccatc
     1561 gagcgcgcgg tcggcacccc gaaatcggcc ggcggggtag tgaactcgac ccccgcggcg
     1621 cgcaggtcgt ggatgtagaa ctggcccagc tggtcgggcg cgacctggcc gatgaagccg
     1681 gcccggccgc cgagcgccgc gaccccggcc gcggtgttcc ccgccgaccc gccgctgacc
     1741 tcgcgcgccg ggcccatgtg cgcatagagg cgctcggcct cctcggcgtc gatcagccgc
     1801 atcgagccct tggtcagccc ctcggcgtcg aggaacgcgt cgtcggcatc ggcaatcacg
     1861 tcgacgatcg cgttgccgat cgccaggacg tcgaggcggg gggagggcat cggcgccgcc
     1921 tagcggcggc cgcggcccgc ggcaagcttg cctcggccgg tgcgcgccgc catcatcggg
     1981 caatgcgccg cgctgtcgcc ttcgccttgc tgctggtcgc cggctgcgcg accgccccgc
     2041 agccgcagcc cgagcagccc cggcccctgc ccgcaacgcc cgcacctcag cttcgcggcg
     2101 aactcatcgg cctgaccgcg tcggagctgg tgcagcggct cggcaatccg gcgctgcaaa
     2161 tccgcgaggg ccagagcctc aagctccagt tccggggctc gcgatgcatc ctcgacgcct
     2221 acctctatcc gccgcgcgac tcctcggggc cggagcgggt cgcccatgtc gatgcccggc
     2281 tgcgctcggg ggcggaaacc gatccgaggg tctgcgtcgc gacgctgtcg cggacctagg
     2341 ccagctgctc cattgcccag gccgccgcct cggccacgac cgggtcggag tcggcgaggt
     2401 gcggctcgac gtccttgacc agcccggggt cgccgctgtt gcccgccgcg atcaggcagt
     2461 tgcggaccat gcgcgcccgt ccgatgcgct tgatcggcga gcccgcgaac atctcgcgga
     2521 acgcggcgtc gtcgagcgcc agcaggtcgc cgagccgcgg agcggccagc tcggcgcgcg
     2581 gcaggaacgc ccggttggcc gcggcggcgg cggcaaagcg gttccacggg cacaccgcga
     2641 ggcactcgtc gcagccgtag atgcggttgc ccatcgcccg ccggaactcg ttggggatcg
     2701 gccccgaatg ctcgatggtc aggtacgaga tgcagcgccg ggcatcgatc cggtgggcgg
     2761 cgtcgaacgc ctgcgtcggg cagacgtcga ggcagcgcgt gcagctgccg caatggtgcg
     2821 acgcgggctc gtcgggctcg agctcgaggc tggtgaggat gatcccgagg aacagccagc
     2881 tgccgtgctc gcgcgacacg aggttggtgt gcttgccctg ccagccgatg ccggcggccg
     2941 cgctcagcgg cttttccatc accggtgcgg tgtcgacgaa caccttgagc tcggacggcg
     3001 cgcgctcgac gatgaagcgc gccagcgcct tcagcgcctt cttgacggtc ttgtgatagt
     3061 cgccgccctg ggcataggcc gagatccggc cgatcgccgg ctcggcggcc agcgcccgcg
     3121 gatcggtcgc cggcgcatag ctcatgccga gcgcgatcgc cgagcgcgcg tcgggccaca
     3181 gggcgagcgg cgaggcgcgc tggtcggcgc gctcctccat ccagcccatc gtcccgtgat
     3241 ggccggcggc gagccagcgc ccgagatcga gccccgagtc cgccgccgca tcggcgcgtg
     3301 cgaacccaca ggcgacgaag ccgaggcgct cggcttcggc gcggattgca tccttgaggc
     3361 gttggcttct atccacgagc gcgaattaca ctcttgtccg ccgtttctgt aggaatttca
     3421 gtgcttcgaa tggcccagca cgaaccgatg accgaagacc cggcggtcga ggccgtcgac
     3481 ctggtcaagg atttcggtga aacgcgggcc ctcgacggca tcagcctcgc ggtcccggcg
     3541 ggctcgatct acgggctgct cggacccaat ggcgccggca agacgaccgc gctgcgcgtc
     3601 atgctcggga tcatcgaccc gtcgtccggg acccgccggg tgctcggccg cgagcagccg
     3661 ctcgatgcgg cctgggaggt tggctatctg cccgaagagc gcggcctcta ttcggggatg
     3721 accgcgcgcg aggcgatcgc cttcatgggc gccctgcgcg gcctggcgct caaggagggc
     3781 cggcgccgcg ccgacgagct gctcgccgct cacggcctcg gcgaatgggc tcgaaagccg
     3841 atccgcaacc tgtccaaggg catggcccag acggtgcagc tgctcggcac catcgtccat
     3901 cgcccccgac tgctggtcct cgacgagccg ttcgccgggc tcgacgcgat caaccaggcc
     3961 cggctagagg aactgatccg gggcagggcg cgcgacgggg tcacggtgat cttctcgacg
     4021 cacgtgatcg cccacgccga gcggctgtgc gagcggatcg cgatcatcgc cggcggcaag
     4081 atcgcgttcg aaggcgcggt cgacgaagcg cgcgaccggc tgcggccgat cgtccggctg
     4141 cgcacccgct cggccgaggg gccgtggcgc tcggcgatcc cagcgaacgc ccgccgcgag
     4201 cgcggcgagt gggtgttcga gctgccgccc ggcggccccg aaccgctgct caaggcgctg
     4261 atcgacggcg gagcgggcat cgagacgctg gcgatcgagc gcccgggcct gcacgacgcc
     4321 tttgtcgcca tcgccggcga tgcggcggcg gcggcgatgg gcgatctggg cgagcccggc
     4381 tgatgtcgct gctgcgctcc gccttcgtca tcgcccgccg cgacttcacc gcgacgatct
     4441 tctcgcgggc ctttttcttc ttcctcctcg ggccgctgtt cccggtgctg atgatggtga
     4501 tcttcggaag catcggcggg gcggcggtca gcgagatcga gcggccggtc gttgccgtcg
     4561 tcgcctcgga cgccgatttc caggcgctcg ccgaagccag gcggcggctc gacgaagcgg
     4621 tcgacccgcc ggcgatgatc acgctgcgcc acgtcccgcc agagcccgac tcggccgcgc
     4681 agcgggcgcg gctgctggcc gcgcaatcgc cgccggtgat cgccgtgctg gaaggcggga
     4741 tcgaccggcc gcgcgcgacc ggtgcgctgc ttcccgacgg caccaccgtt cgccagctgc
     4801 gcttcctgat cgaggatgcg cgccgcgcac agacggcgcc gccgatcgcc gaggccaacg
     4861 tcctgctgac caggacccag cgctcgccgg gcttgctcgc gccggcccgg gcgatgaccg
     4921 ccaggatggg ccagatggtg ctgttcctgc tcagcctgct gctgtcgggc atgctgctgt
     4981 cgcagctgat cgacgagaag gccaacaaga tcatcgaagt cctcgccgcg gcggtgccga
     5041 tcgacgcgat cttcctcggc aagctgtccg cgatgctggc catgtcgctg ctcggcctgg
     5101 cggtctggac ctcgctggga gccgcggcga tcgcggtgat gaccggccag agcctgtcgg
     5161 cgctgccgtc gccggcggtc ggctggcccg ccttcctgac cctggcggtc gtctatttca
     5221 tgatgagcta tctgctgctc ggctcggtgt tcctcggcat cggcggccag gcgtcgaccg
     5281 cccgcgaagt ccagaccctg tcgatgccgg tgaccatggc gcaggtggtg gtgttcgcga
     5341 tcgccgcgag tgcggtcgga gcgccgaaca gcgcgcgggc gctggccgcc gtggtcttcc
     5401 cgctgtcgtc gccgttcgtg atgatcgcgc gtgccgccga gctgccgcag atctggccgc
     5461 acctgttggc gatcgcctgg cagtctttgt gggtcgccct gatcctgcac ttcgcgtcgc
     5521 gagttttccg ccgaatcgtg ctgaagtccg ggccttcgcg cccgttctgg cggcgcgcgg
     5581 ccaaagccta gttcagcgcc gcgcgcgcca tctcttcgag cttcgccatc aacgcccggt
     5641 gcgggaacgg gccgtggctg atccgggcga caccggcttg cgctaccgcc tgggcgtcgg
     5701 gcgcgccggg gaaggcgatg acgttcaacg gcagcggcac gtcgcggacg atccgctcga
     5761 tctgccgcgg gtcggcgagg cgcgggacga agaagccgct cgcgccggcg tcggaaaacg
     5821 ccttgccgcg ctcgatcgcc gcgtcgacca gcgccgaatc gtgctgctga gtcttcaggt
     5881 agaggtcggt gcgggcgttg atgaagaagt catcgccgac cgcgcgctgg atggcttcga
     5941 tgcgccgcac ctgaagctcg agcgggtgga caccctcgcc cccgatcacc tggtcctcga
     6001 agttgcagcc gaccgcg
//
LOCUS       DASTZO010000066         6712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_840079,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000066 DASTZO010000000
VERSION     DASTZO010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6712
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840079"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /gene="rsmA"
                     /locus_tag="VFU20_01705"
     CDS             <1..667
                     /gene="rsmA"
                     /locus_tag="VFU20_01705"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU5285208.1"
                     /translation="VGPGPGGLTRALLDAGANVVAVERDRRCRPALAELERAYPARLK
                     VVEGDAMRVDERFEAGDGAHVVANLPYNVGTALLLRWLGGDWPPWWRSLTLMFQKEVA
                     ERIVARPGSEHYGRLSVAAQWRAQPRIALSVNRSAFVPPPKVTSAVVHIVPAAMPAGV
                     DPSVLERLTEAAFGQRRKMLRSSLKALPGALNALEELGIDSGRRAETLSVEEFVAVAR
                     VLG"
     gene            complement(664..1164)
                     /locus_tag="VFU20_01710"
     CDS             complement(664..1164)
                     /locus_tag="VFU20_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285209.1"
                     /translation="MMRLLHRIALAGLAGLLIAAPVSSQKPDDQIHPRSLELLRQGET
                     LLAGGKFVEADDALEAALAVDPRNRAAFVVMARVAQKQKLFGQAIRFTNKALALEPRD
                     LDALAVQGEAMVELGAVPRARDNLARLQKLCPSGCRQLAALSAAISRGPTAVASVKPP
                     PSPKTN"
     gene            complement(1190..2365)
                     /locus_tag="VFU20_01715"
     CDS             complement(1190..2365)
                     /locus_tag="VFU20_01715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEU5285210.1"
                     /translation="MTPAARLQAAVEILDEVIASARDDGPPADVIVTRYFKARRYAGS
                     GDRRAVRELVFRAIRRSAERPGNGRAAALGLANDDPELLALFGEGRGPPAIAEGEEAS
                     PAAAVPSWLEAEVSPLVSSAERPALLERAPLDLRVNLARAERDQLLGQFPGAEPTRLS
                     PWGLRLPPDSRVDDHPAFIDGLVEVQDEGSQLIALACEPRDGMRILDLCAGGGGKALA
                     LAAAAPGAQILATDTNRSRLSKLPPRAAKAGARIETRLLDTPRELEALADWADSADLV
                     LVDAPCSGSGTWRRNPEGRWRLTPGRLERLVRLQAHLLDIGAAMVRPGGRLVYAVCSI
                     LTAEGEGQVAQFLGRRSSWVAQETPIQAGRLAGAGKLLTPGHDGTDGFFVARLARPC"
     gene            complement(2369..3847)
                     /gene="guaB"
                     /locus_tag="VFU20_01720"
     CDS             complement(2369..3847)
                     /gene="guaB"
                     /locus_tag="VFU20_01720"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU5285211.1"
                     /translation="MAELNAAPDIPMGLTFDDVLLQPLESSVLPSQADTSTFLTRAIP
                     LNIPLLSSAMDTVTEADMAIALAQLGGIGVLHRNLSVDEQSAAVRAVKRFESGMVVNP
                     ITMSPDQTLAEALELMKSNRISGIPIVEKSGKLCGILTNRDVRFAENPRQPVSELMTR
                     DNLATVPLGTSQEEAQRILHQRRIEKLLVVDDADHCVGLITVKDIEKSVAAPFATKDS
                     EGRLRVAAATTVGDSGFARSEALIDAGCDCIVIDTAHGHNRDVGTAVERVKTLSNSVQ
                     VVAGNIATADAAKALIDSGADAIKVGIGPGSICTTRIIAGVGVPQLTAILSAAAAAAN
                     SGVPVIADGGIRTSGDIAKALAAGASAVMVGSLLAGTEEAPGETFLYQGRTYKSYRGM
                     GSVGAMARGSADRYFQQDIRDHLKLVPEGIEGQVPYKGPVRDIVHQLVGGVKAAMGYT
                     GSASIAELQRRAKFIRITNAGLSESHVHDVAITREAPNYPTR"
     gene            3961..4425
                     /locus_tag="VFU20_01725"
     CDS             3961..4425
                     /locus_tag="VFU20_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5285212.1"
                     /translation="MKAVLTAIAVAGVGSGAIAQGTRHTAVTSPDGLTWQASPAFPKG
                     VLVATLAGNPAAKGDTVVMRIKFPPHFTMPPHTHPYSEVVTVISGSIGTNGGTTVEKA
                     GGLLPPGSLWVYPARHPHYAWTGDYEAVLQVQFTGPSGIDYVNPADDPRRRK"
     gene            complement(4444..5250)
                     /locus_tag="VFU20_01730"
     CDS             complement(4444..5250)
                     /locus_tag="VFU20_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HEU5285213.1"
                     /translation="MTLDLFAVRSMLFLPASNPRAIARAREAGSDLVVLDLEDAVKPG
                     DKEQARSAALDAVASPWPMPVAIRINGVGSEWHSLDVDAVSRSSADLAVVPRAASAHL
                     VRGVREALDKPVLAMIETASGVLAAPEIAKESAGLIAGTNDLAADLRLPPDAGREPLQ
                     MALQSIVLAARAAGIAAFDGVFNGLTDMDRFSAEAAEGRRLGFDGKSLIHPDQVEACH
                     RAFAPSEAEIDRARRLVEAFHGGAERFEDRMIERMHVEAAERLLERSASR"
     gene            5322..6335
                     /locus_tag="VFU20_01735"
     CDS             5322..6335
                     /locus_tag="VFU20_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU5285214.1"
                     /translation="MLTTFMIAIAVLALLFVMMGVKIVHQGFRYTIEHFGRFVRVAQP
                     GFNFVPPFFYRVGHKINVMEQVVDIPGQEIITRDNAMVAVDGVVFFQVLDAAKAAYEV
                     SDLYTAIMALSTTNLRTVMGSMDLDETLSKRDEINARLLAVVDHATEAWGVKITRVEL
                     KDIRPPADIVNAMTRQMKAEREKRAAILESEGMRASEILRAEGEKQAKILQAEGLKEA
                     AFREAEARERSAEAEANATRSVSDAIEAGSGQAINYFVAQKYVEALGKFATSPNAKTI
                     LFPVEATQLMGTLGGIGELAREVFGGEDRPPRPPAAPKPKREPVATRGAPPSISGVPR
                     IEG"
     gene            6335..>6712
                     /locus_tag="VFU20_01740"
     CDS             6335..>6712
                     /locus_tag="VFU20_01740"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEU5285215.1"
                     /translation="MLDNLEPGWLWMIGGVLLLIAEIIAPGFFLVFIGAAALATGAFA
                     LLFGLGTASQLALFALYSLIAVMVGRRVYSNASVDSSDPFLNDRSARLVGKVVSVVEP
                     VDAHGGRVRVGDSEWSARGGPAAA"
BASE COUNT          980 a         2391 c         2336 g         1005 t
ORIGIN      
        1 ggtcgggccc ggcccgggcg gcctgacccg ggcgctgctc gacgccggag cgaacgtcgt
       61 cgcggtcgag cgcgaccgcc gctgcaggcc ggcgctcgcc gagctcgagc gggcctatcc
      121 ggcgcgattg aaggtcgtcg agggcgatgc gatgcgggtc gacgagcgct tcgaggccgg
      181 cgacggcgcg catgtcgtcg ccaacctgcc ctacaatgtc ggcaccgctc tgctgctgcg
      241 ctggctcggc ggcgactggc cgccgtggtg gcgctcgctg accctgatgt tccagaagga
      301 ggtcgccgag cgaatcgtcg ctcgccccgg cagcgagcat tacgggcggc tgtcggtggc
      361 cgcccagtgg cgggcccagc cgaggatcgc gctcagcgtc aaccgctcgg cgttcgtgcc
      421 gccgcccaag gtgacctcag cggtggtcca catcgtgccg gcggcgatgc cggccggagt
      481 cgacccgagc gttctggagc ggctgaccga ggccgcgttc ggccagcgcc gcaagatgct
      541 ccgctcgagc ctcaaggcat tgcctggcgc gctcaatgcc ctggaggagc ttggaatcga
      601 ctccggccgc cgagccgaga cgctcagcgt cgaggagttc gtcgccgtcg cgagggtgct
      661 aggctaattg gtcttggggc tcggcggggg cttgaccgac gccaccgcgg tcggcccgcg
      721 gctgatcgcc gccgacaggg ccgccagctg ccggcacccg ctcgggcaca gcttctggag
      781 cctggcgaga ttgtcgcggg cgcgcggcac cgcgcccagc tcgaccatcg cctcgccctg
      841 gacggccagc gcgtcgaggt cgcgcggctc caatgccagc gccttgttgg tgaagcggat
      901 cgcctggccg aacaatttct gcttctgggc gacgcgcgcc atcaccacga acgcggcccg
      961 gttgcgcggg tcgaccgcca gcgccgcctc gagcgcatcg tcggcctcga cgaacttgcc
     1021 gccggcgagc aaagtctcgc cctggcggag cagctcgagc gagcggggat ggatctggtc
     1081 gtccggcttc tggctggaca ccggcgcggc aatcagcagc ccggcaagtc cggccaacgc
     1141 aatgcggtgc agcaggcgca tcattctctc cagttcgctc gccgcccacc taacatggtc
     1201 gggccagccg cgcgacgaaa aagccatcgg tgccgtcatg gccgggggtc agcaacttgc
     1261 cggcacccgc caaacgcccg gcctggatag gtgtttcctg tgcaacccag gatgaacggc
     1321 ggcccaggaa ttgcgcgacc tggccctcgc cttcggcggt gaggatcgag cagaccgcat
     1381 agaccagccg accgcccggg cggaccatcg ccgcgccgat gtcgagcaga tgcgcctgca
     1441 gccggacgag ccgctcgagc cgcccgggag tcagccgcca gcggccttcg gggttgcgcc
     1501 gccaggtgcc gctgcccgag cagggcgcgt cgaccagcac caggtcggcg ctgtccgccc
     1561 agtcggccag cgcctcgagc tcgcgcggag tgtcgagcaa ccgggtctcg atccgggcgc
     1621 cggccttcgc ggcgcgcggc ggcagcttcg agaggcgcga gcggttggtg tcggtggcga
     1681 ggatctgcgc gcccggagcc gcggccgcga gcgccagcgc cttgccgccg ccgcccgcgc
     1741 acaggtcgag gatgcgcatc ccgtcgcgcg gctcgcaagc cagcgcgatc agctggctgc
     1801 cctcgtcctg gacctcgacc agcccgtcga tgaacgccgg atggtcgtcg acccggctgt
     1861 caggcggcaa ccgcagcccc cacgggctga gccgagtcgg ctcggcgccg gggaactggc
     1921 cgagcaattg gtcgcgctcg gcccgggcaa ggttcacccg aaggtcgagc ggagcgcgct
     1981 cgagcagcgc cggccgttcg gcggacgaga ccagcggcga gacctcggcc tcgagccacg
     2041 acgggaccgc cgccgcgggg ctcgcctcct cgccctcggc gatcgccggc ggcccgcgcc
     2101 cctcgccgaa cagggcgagc agctccggat cgtcgttcgc cagcccgagc gccgcggcgc
     2161 gcccgtttcc cggccgttcc gccgacctgc ggatcgcccg gaacaccagc tcgcggaccg
     2221 cccggcgatc gcccgagccg gcgtagcggc gggccttgaa atagcgcgtg acgatcacat
     2281 cggcgggcgg cccgtcgtcg cgggccgagg cgatcacctc gtcgaggatt tcgaccgccg
     2341 cctgcagccg ggcggccggc gtcatggcct agcgcgtcgg gtaattgggc gcctcacggg
     2401 tgatggcgac gtcgtggacg tggctttccg acaggccggc gttggtgatt cggatgaact
     2461 tcgcgcgccg ctgcagctcg gcgatgctgg ccgagccggt gtagcccatc gccgctttga
     2521 ccccgccgac cagctggtgg acgatgtcgc gcaccgggcc cttgtacggc acctgcccct
     2581 cgatgccctc gggcaccagc ttcaaatggt cgcggatgtc ctgctggaaa tagcggtcgg
     2641 ccgagccgcg cgccatcgcc ccgaccgagc ccatgccgcg gtagctcttg taggtccgcc
     2701 cctggtacag gaaggtttcg cccggcgctt cctcggtccc ggcgagcaag gatccgacca
     2761 tcaccgccga cgcgccggcc gccaacgcct tggcgatgtc gccgctggtc cggatgccgc
     2821 cgtcggcgat caccgggacg ccgctgttcg ccgcggccgc ggccgccgac aggatcgcgg
     2881 tcagctgcgg caccccgacc ccggcgatga tccgggtcgt gcagatcgag cccgggccga
     2941 tcccgacctt gatcgcgtcg gcgcccgaat cgatcagcgc cttcgccgca tccgccgtgg
     3001 cgatgttgcc ggccaccacc tggaccgagt tggacagggt cttcacccgc tcgaccgccg
     3061 tcccgacatc ccggttgtgg ccgtgcgcgg tgtcgatgac gatgcagtcg cagccggcgt
     3121 cgatcaaagc ctccgagcgc gcgaagcccg agtcgccgac cgtcgtcgcc gcggcgaccc
     3181 gcagccgccc ttcggaatcc ttggtcgcga acggcgccgc gaccgatttc tcgatgtcct
     3241 tgacagtgat cagcccgacg cagtggtcgg cgtcgtcaac caccagcaat ttctcgatcc
     3301 gccgctggtg gaggatccgc tgcgcttcct cctggctggt tccgagcggc acggtggcca
     3361 gattgtcgcg ggtcatcagc tccgacaccg gctggcgcgg gttctcggcg aagcggacgt
     3421 cgcggttggt caatatgccg cacagcttgc ccgatttctc gacgatcggg atgccgctga
     3481 tgcggttgga cttcatcagc tcgagcgcct cggccagggt ctggtcgggg ctcatggtga
     3541 tcgggttgac gaccatcccg ctttcgaacc gcttgaccgc ccggaccgcg gccgactgct
     3601 cgtcgaccga caggttgcgg tggagcacgc cgatgccgcc cagttgggcg agcgcgatcg
     3661 ccatgtcggc ctcggtgacc gtgtccatcg ccgaggacag caagggtatg ttgagcggga
     3721 tcgcccgggt caggaaggtg ctggtgtcgg cctggctcgg gagcaccgag gattcgagcg
     3781 gctggagcag cacgtcgtcg aaggtcagcc ccatcgggat gtcgggagct gcgttgagtt
     3841 cggccatatc ggcccatgcc aaggaaacgg cgattcgcca agtggccggc cggcccgcta
     3901 cgcttggaag tgcctccctg cgaggtctag aaagtccctc gaagccaatt ggaggcgtga
     3961 atgaaagccg ttctgaccgc gatcgccgtt gccggcgtgg gaagcggggc aatcgctcag
     4021 ggcacccggc acaccgccgt caccagcccc gacggcctca cctggcaggc cagtccggcc
     4081 tttcccaagg gcgtgctggt cgcgaccctg gccggcaatc cggcggccaa gggcgacacc
     4141 gtcgtcatgc gcatcaaatt cccgccgcac ttcaccatgc cgccgcacac gcacccttat
     4201 tccgaggtgg tgacggtgat cagcggctcg atcggcacca acggcggaac cacggtcgag
     4261 aaggccggcg gcctgctccc gcccgggtcg ctgtgggtct atccggccag gcacccgcat
     4321 tatgcctgga ccggcgacta tgaagcggtg ctgcaggtcc agttcaccgg cccgagcggc
     4381 atcgactacg tcaacccggc cgacgacccg cgccggcgga agtgatcgct tgggcgctgc
     4441 tggctaacgg ctggccgagc gctcgagcag ccgctccgcc gcctcgacgt gcatgcgctc
     4501 gatcatccgg tcctcgaacc gctcggcgcc gccgtggaac gcctccacca gccgccgggc
     4561 gcggtcgatc tcggcttcgc tgggcgcgaa cgcgcggtgg cacgcctcga cctggtcggg
     4621 gtggatcagg ctcttgccgt cgaagccgag gcggcggcct tccgcggcct cggccgagaa
     4681 gcggtccatg tccgtgagcc cgttgaacac gccgtcgaac gccgcaatcc cggcggcgcg
     4741 cgcggcgagc acgatcgact gcagcgccat ctgcagcggc tcgcgccccg catcgggcgg
     4801 cagccgcagg tcggcggcga ggtcattggt gccggcgatc agcccggcgc tttccttggc
     4861 gatctccggg gcggccagca ccccggacgc ggtctcgatc atcgccagca ccggcttgtc
     4921 gagcgcctcg cggacgccgc gcaccaaatg agccgaggcg gcgcgcggca cgaccgcgag
     4981 gtcggccgac gaccgcgaca ccgcatcgac gtccagcgaa tgccattcgc tgccgacgcc
     5041 gttgatccgg atcgcgaccg gcatcggcca gggcgacgcc accgcgtcga gcgccgccga
     5101 ccgcgcctgc tccttgtccc cgggcttgac cgcatcctca aggtcgagga cgaccagatc
     5161 tgaccccgcc tcgcgcgccc gggcgatcgc gcgcgggttc gaggccggca ggaacagcat
     5221 ggagcggacc gcgaacaggt cgagggtcat gccgatttct ctttcacgcg gcctcaggcc
     5281 gtcgcatcat aagccgacaa tatgggagcg cgcacgtgga tatgctgacg acctttatga
     5341 ttgcaatcgc cgtcctggcg ctgctgttcg tgatgatggg cgtcaagatc gtccaccagg
     5401 gcttccgcta caccatcgag catttcggcc ggttcgtgcg cgtcgcccag cccggcttca
     5461 acttcgtgcc gcccttcttc tatcgcgtcg ggcacaagat caacgtgatg gagcaggtcg
     5521 tcgacatccc cggccaggag atcatcaccc gcgacaatgc gatggtcgcg gtcgacgggg
     5581 tggtgttctt ccaggtgctc gacgcggcca aggcggccta tgaggtcagc gacctctaca
     5641 ccgcgatcat ggcgctatcg acgaccaatt tgcgcaccgt aatggggtcg atggacctcg
     5701 acgagaccct gtcgaagcgc gacgagatca acgcccggct gctggccgtg gtcgaccatg
     5761 cgaccgaggc gtggggcgtc aagatcaccc gcgtcgagct caaggacatc cggccgccgg
     5821 ccgacatcgt caacgcgatg acccgccaga tgaaggccga gcgcgagaag cgcgcggcga
     5881 tcctcgagtc cgaaggcatg cgggcgtccg agatccttcg cgccgagggc gagaagcagg
     5941 ccaagatcct gcaggccgag ggcctcaagg aagccgcgtt ccgtgaggcc gaagcgcgcg
     6001 agcggtcggc cgaggcggaa gccaatgcga cccgctcggt cagcgacgcg atcgaggccg
     6061 gaagcggcca ggcgatcaac tatttcgtcg cccagaaata tgtcgaggcg ctcggcaagt
     6121 tcgccacctc gcccaacgcc aagacgatcc tgttcccggt cgaggcgacc cagctgatgg
     6181 gtactctggg cggcatcggc gagctcgccc gcgaagtgtt cggcggcgag gaccggcccc
     6241 cgcggccgcc cgccgcgccc aagccgaagc gcgagcccgt cgcgactcgc ggagcaccgc
     6301 cctcgatctc cggcgttccg aggatcgaag gctgatgctc gacaatctgg agcccggctg
     6361 gctgtggatg atcggcggcg tgctgctgct gatcgccgag atcatcgcgc ccggtttctt
     6421 cctcgtgttc atcggcgcgg cggctctggc gaccggtgcg ttcgccttgc tgttcggcct
     6481 cggcaccgcc tcgcagctcg ccttgttcgc gctctacagc ctgatcgcgg tgatggtcgg
     6541 gcggcgcgtc tactccaacg cctcggtcga cagcagcgac cccttcctca acgaccgctc
     6601 ggcgcggctg gtcggcaagg tcgtgtcggt ggtcgagccg gtcgatgcgc acggcggccg
     6661 ggtgcgggtc ggcgacagcg aatggagcgc tcgcggcggc cccgccgcgg cc
//
LOCUS       DASTZO010000067         6694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_919327,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000067 DASTZO010000000
VERSION     DASTZO010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6694
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919327"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="VFU20_01745"
     CDS             <1..372
                     /locus_tag="VFU20_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285216.1"
                     /translation="LDSSRRDRRGEANDLASEAAAAASDVTGEIIGAPVHRHLSGGGA
                     AADDFQRIKGVGPKLADMLAARGFLRFEQLAHLTPQEIDMIDEQLGPFRGRLRRDRIV
                     EQAAYLARGDVDGFEQQFGKL"
     gene            complement(379..1722)
                     /locus_tag="VFU20_01750"
     CDS             complement(379..1722)
                     /locus_tag="VFU20_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU5285217.1"
                     /translation="MSAPLLPFPWLDLVLILALIALNGVLSMSELAIVSSREARLKAM
                     AKSGSAGARRALELAAEPGRFLSTVQMGITLVGILAGAYSGATLGEPVGQRLALLGIE
                     PETASTIGFAIVIVVVTYASLVIGEIVPKQFALRSPEPIAAIMARPLDWLSKITAPFV
                     WLLDKTSALIFRALGLDRESRNQVTAEELHLVVAEAQTAGVLEESERAIISGIVRLAD
                     RPVREVMTPRTDVDWIDIRATSDEIRSALREIPHSRIPVADGSVDNIVGVIQARDLLT
                     RALDGQPLELQSLCRSAPVIPDLMDAMDALAVLRAADVPLALVHDEYGHFDGIVTPGS
                     ILAALAGAFAHDVDEDEHPPLVEREDGSWLVSGAASADLLSDRLGVRLPDERDFSTVA
                     GFALSVLKHLPDTGERFKFDGWSFEIVDMDGRKIDKLIASRAKAKKREAKAESVG"
     gene            1871..2530
                     /locus_tag="VFU20_01755"
     CDS             1871..2530
                     /locus_tag="VFU20_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU5285218.1"
                     /translation="MRRFRTLAALAISAPLLATAGCGIGPFAPPPPPPPAPTIVVPVV
                     PAYVHSDLIGGRAARVKKARAAGIKPLTAAAAWDYVGRQETELRRQTAGTGVDVIRSG
                     ELLLLRLPASGTFDVGSSDIKPQAVSTVAEIGLTLKKFNQSLVDVLGHTDSTGSAAAN
                     LALSQKRAESVARQLRAKGVSAARIATRGYGAGHPISDNVTEPGRAMNRRVEIKLVPL
                     R"
     gene            complement(2543..2995)
                     /locus_tag="VFU20_01760"
     CDS             complement(2543..2995)
                     /locus_tag="VFU20_01760"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEU5285219.1"
                     /translation="MTFPLPPPMRRALDLAAEAAADGEVPVGAVVTLGDAIIAEARNA
                     MRGRLDPTAHAEMVAIRAAAEKLGSPRLDRCTLWVTLEPCAMCAAAMAVSRIAALRFG
                     AEDPKGGGVIHGARIFALPTCHHRPDVLGGIGEAESAALLRDFFAARR"
     gene            complement(3043..3351)
                     /locus_tag="VFU20_01765"
     CDS             complement(3043..3351)
                     /locus_tag="VFU20_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285220.1"
                     /translation="MRAILLVLILAVVALIALVATGLVDINQLRGAKAPDVDVSRNGV
                     TATGGQAPAFDVETGSVSVGTRDATVKVPELRVNPAGNDAAPAASQPAQNQAAPVNGM
                     "
     gene            3514..3807
                     /gene="rpmB"
                     /locus_tag="VFU20_01770"
     CDS             3514..3807
                     /gene="rpmB"
                     /locus_tag="VFU20_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU5285221.1"
                     /translation="MSRVCELTGKGRQVGNNVSHANNKTKRTFLPNLQNVTLISDALG
                     QSVKLRVSTHGLRSVEHVGGLDNWLTKTSDEKLSARARKLKREIAKKAKESAA"
     gene            complement(3825..4487)
                     /locus_tag="VFU20_01775"
     CDS             complement(3825..4487)
                     /locus_tag="VFU20_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HEU5285222.1"
                     /translation="MYRVLPILTEAEVEQCRAIAASAPFVDGRISNPHNTAKANEQLH
                     DAAAYQKSSELLKRAMARSEEFREFAFPVAMAPPMMTRYRPSMRYGAHADAAFIQLPG
                     ATIRSDLSCTIFLNDPKDYDGGELHIRIGDAELTFKLQPGEAIVYPSDTLHQVEPVTR
                     GERLVAITFIQSRIQDPFRRNLLFELNEVAALEGLKMDPANFNRLQLVQQNLLRFWGE
                     KP"
     gene            complement(4499..5443)
                     /locus_tag="VFU20_01780"
     CDS             complement(4499..5443)
                     /locus_tag="VFU20_01780"
                     /inference="COORDINATES: protein motif:HMM:NF024841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HEU5285223.1"
                     /translation="MNPEREVQPKFRIRELAAGLLLLAAIALWDLALARIPNRAEFGW
                     RAARIEARPLRLDSRAFAPLRLAGAWELTSADPRFGGVSALAIDRGRLLALTDSGALV
                     RFSPRRQMAIIGELPGGPGDARFKRYRDSEALVRDPLGRGWWVAFENRHQLWLYDPGF
                     RRALRRIDLGKRGWRANRGIEGAVAEGGALLLVHEEGNHVLRVAGSRARTLPIAHAGG
                     RISDAAAIGAGRSIAVERRASPIGFRNALVILERSGSGYRFGRRVALPLGPLDNVEAV
                     AVERRAGGGLRLWLMTDDNFQPPLRTLLIALDWPAPRQ"
     gene            complement(5585..>6694)
                     /locus_tag="VFU20_01785"
     CDS             complement(5585..>6694)
                     /locus_tag="VFU20_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobT"
                     /protein_id="HEU5285224.1"
                     /translation="GDEGSPSGGDMERRGEEADEGDDQEQGSDDVDLGDQDGSRDDDI
                     SESAQAAAGRRNWELAPETDYRAFTTRFDEVVEAEDLCDVEELGRLRAYLDQQMASSQ
                     SVVTRLANRLQRRLMAQQARSWEFDQEEGLLDSARLARVIVNPAHSLSYKVERDMEFR
                     DTIVSVLIDNSGSMRGRPIAIAAICADILARTLERCGVATEVLGFTTRAWKGGQSREA
                     WLAEGRPGHPGRLNDLRHIIYKRADEPYRHARRNLGLMMREGLLKENIDGEALLWAHH
                     RLIARPEERRILMVISDGAPVDDSTASANGGTYLERHLRQVIEWIERRSPVELIAIGI
                     GHDVTRYYERAVTIMDADQLAGAMIEQLADLFDAG"
BASE COUNT         1021 a         2335 c         2345 g          993 t
ORIGIN      
        1 ctggattcga gccggcgcga ccggcgcggc gaagccaacg acctcgcctc cgaggcggcg
       61 gcggcggcga gcgacgtcac cggcgagatc atcggcgcgc cggtccaccg ccacctgtcc
      121 gggggcggcg cggcggccga cgatttccag cgcatcaagg gcgtcgggcc caagctcgcc
      181 gacatgctcg cggcgcgcgg cttcctccgg ttcgagcagc tggcccattt gaccccgcag
      241 gagatcgaca tgatcgacga gcaattgggg ccgttccgcg gccgcctgcg ccgcgaccgg
      301 atcgtcgagc aggccgctta cctcgcgcgc ggcgacgtcg acgggttcga gcagcagttc
      361 ggcaagctgt agggcccgct agccgacgct ttcggccttg gcctcgcgct ttttcgcctt
      421 ggcccgcgag gcgatcaatt tgtcgatctt gcggccgtcc atgtcgacga tctcgaacga
      481 ccagccgtcg aacttgaacc gctctccggt gtcggggaga tgcttcaaca ccgacagcgc
      541 gaagccggcg acggtcgaga aatcgcgctc gtcgggcagg cgcaccccga gccggtcgct
      601 caggaggtcg gcgctggcgg cgcccgacac cagccaactg ccgtcctcgc gctcgaccag
      661 cggcggatgc tcatcctcgt cgacgtcgtg ggcgaaggct ccggccagcg ccgcgaggat
      721 gcttccgggc gtgacgatgc cgtcgaaatg gccatattcg tcgtggacca gagccagcgg
      781 gacgtcggcg gcgcgaagca ccgcgagcgc gtccatcgcg tccatcaggt cggggatcac
      841 cggcgccgag cggcacagcg actggagctc gagcggctgg ccgtcgagcg cgcgggtcaa
      901 caggtcgcgg gcctggatca ccccgacgat attgtcgacg gagccgtcgg cgaccgggat
      961 ccggctgtgc gggatctcgc gcagcgcgct gcggatctcg tcgctggtcg ctctgatgtc
     1021 gatccagtcg acgtcggtcc gcggggtcat cacctcgcgc accgggcggt cggcgagccg
     1081 gacgatgccc gagatgatcg cccgctcgct ttcctcgagc acgccggcgg tctgggcttc
     1141 ggcgacgacc aggtgcaatt cctcggcggt gacctggttg cgcgattcgc ggtcgaggcc
     1201 gagcgcccgg aaaatgagcg cgctggtctt gtcgagcagc cagacgaacg gagcggtgat
     1261 cttcgacagc cagtcgagcg gccgcgccat gatcgccgcg atcggctcgg gcgagcgcag
     1321 ggcgaactgc ttggggacga tctcgccgat caccagcgac gcgtaggtga cgacgacgat
     1381 gacgatggcg aagccgatgg tgctcgcggt ctcgggctcg attccgagca gcgcgagccg
     1441 ctgcccgacc ggctcgccga gcgtcgctcc cgaataagcg ccggcgagga ttccgaccaa
     1501 ggtgatgccc atctgcaccg tcgacaggaa gcggccgggc tcggcggcga gctcgagcgc
     1561 ccgccgggcg ccggcgctgc ccgacttggc catcgccttc agccgcgcct cgcgcgacga
     1621 gacgatcgcc agctcgctca tcgacagaac gccgttcaag gcgatcagag cgaggatcag
     1681 gaccaggtcg agccagggaa atggaagcag gggagcgctc atgggcggag gggcgattta
     1741 gccttcaatg ggggtcgacc acaagcggcc ctggaatcgg ccgggtcgat tcacgtacgg
     1801 aaccaccaag gacgcttgcc ggtttcaggc tcaaatccgg acaggccggt caggacaggg
     1861 gagtcacttg atgcgccgat ttcgaaccct cgccgcgctt gcgatcagcg cgccgctgct
     1921 ggccacggcg ggatgcggga tcggcccctt cgcgccgccg ccgccgccgc cgcccgcgcc
     1981 gacgatcgtc gtcccggtgg tgcccgctta cgtccactcc gacctgatcg gcgggcgcgc
     2041 ggcgcgggtc aagaaggccc gggcggccgg catcaagccg ctgaccgcgg ccgcggcgtg
     2101 ggactatgtc ggccggcagg agaccgagct tcgccgccag accgccggca ccggcgtcga
     2161 cgtcatccgc tcgggcgagc tgctgctgct ccggctgccg gccagcggca ccttcgacgt
     2221 cggcagctcg gacatcaagc cgcaggccgt gtcgaccgtc gccgagatcg gcctcacgct
     2281 gaaaaagttc aaccagagcc tggtcgacgt gctcggccac accgattcga ccggcagcgc
     2341 ggcggccaat ctggcgctgt cgcaaaagcg cgcggaatcg gtcgccaggc agcttcgggc
     2401 caagggcgtg tccgcggccc ggatcgcgac ccgcggctat ggcgcgggcc acccgatctc
     2461 cgacaatgtc accgagcccg gccgggcgat gaaccggcgg gtcgagatca agctggtgcc
     2521 gctgcgctag cggcgccccg ggctaacgcc gagccgcgaa gaagtcgcgc agcagcgccg
     2581 ccgattccgc ttcgccgatt ccgccgagca cgtcgggacg gtggtggcag gtcggcaaag
     2641 cgaagatccg cgcgccgtgg atcactccgc cgcccttggg gtcctcggcg ccgaagcgca
     2701 gcgccgcgat cctggagacg gccatcgccg ccgcgcacat cgcgcacggc tcgagggtca
     2761 cccataacgt gcaccggtcg agccgcgggc tgccgagctt ttcggccgcc gcccggatcg
     2821 cgaccatttc ggcatgggcg gtcgggtcga gccggccgcg catcgcgttg cgtgcctcgg
     2881 cgatgattgc gtcgcccaga gtcaccaccg ctccgaccgg gacctcgccg tccgcggcgg
     2941 cctcggcggc aaggtcgagc gctcggcgca tcggcggcgg cagcgggaag gtcatcgcca
     3001 agcggctagc gcaggccggc cggcgtgtca gccaggcggc ggctacatgc cgttgacggg
     3061 cgccgcctga ttctgggccg gctggcttgc cgcgggagcg gcgtcgttgc cggccgggtt
     3121 caccctcagc tcgggaacct tgaccgtggc gtcgcgggtg ccgaccgaga ccgagccggt
     3181 ctcgacgtcg aacgccggcg cctggccacc ggtcgccgtg accccgttgc ggctgacgtc
     3241 gacgtcggga gccttggcgc cgcgaagctg gttgatgtcg acaaggccgg tcgcgaccag
     3301 cgcgatcagc gcgaccacgg ccaggatgag gacgaggagg atggctctca tgcgctggct
     3361 ccggactgct tctgggatat tggacaacgc aaaactgcgg gcgagtttcc cccgtgccaa
     3421 gttgacgcac ggcatgcaag cggctaacgg gccgctcttt ccgggcgcct tcgagtgctc
     3481 gtccaattca ggtcaaatca ggatcgagac gccatgtcgc gggtatgcga gctgaccggc
     3541 aagggccggc aggtgggcaa caatgtttcc cacgccaaca acaagaccaa gcgcaccttt
     3601 ctgcccaacc tgcagaacgt gaccttgatc tccgacgcgc tcgggcaaag cgtcaagctg
     3661 agggtctcga cccacgggct gcgctcggtc gagcatgtcg gcgggctcga caattggctg
     3721 accaagacca gcgacgagaa attgtcggcc agggcgcgca agttgaagcg cgagatcgcc
     3781 aagaaggcca aggaaagcgc cgcttaagcc ggtgaagcgc tgcgctaggg cttctcgccc
     3841 cagaagcgca gcagattctg ctggacgagc tgcagccggt tgaaattcgc cgggtccatc
     3901 ttcagcccct cgagcgccgc cacctcgttc agctcgaaca gcaggttgcg ccggaacggg
     3961 tcctggatcc ggctctggat gaaggtgatc gcgaccagcc gctcgccgcg ggtgaccggc
     4021 tcgacctggt gaagcgtgtc ggacgggtag acgatcgcct cgccgggctg cagcttgaag
     4081 gtcagctcgg cgtcgccgat ccggatgtgc agctcgccgc cgtcataatc cttggggtcg
     4141 ttcaaaaaga tggtgcagct caaatcgctg cggatggtgg cgccgggaag ctggatgaac
     4201 gccgcgtcgg catgcgcacc gtagcgcatc gacgggcggt agcgggtcat catcggcggc
     4261 gccatcgcca ccgggaaggc gaactcgcgg aactcctcgc tgcgcgccat cgcccgcttc
     4321 agaagctccg agctcttctg ataggcggcg gcgtcgtgca gctgctcgtt ggccttggcc
     4381 gtgttgtgcg ggttgctgat ccggccgtcg acgaacggcg ccgacgccgc gatcgctcgg
     4441 cattgctcga cctcggcctc ggtcaggatg ggcaggacac ggtacatcag cgaaacttct
     4501 attggcgcgg agccggccag tcgagggcga tcagcagagt ccggagcggc ggctggaaat
     4561 tgtcgtcggt catcagccac agccggagcc cgccgcccgc ccggcgctcg accgcgaccg
     4621 cctcgacatt gtcgagcggc ccgagcggga gcgcgacccg gcggccgaag cggtagcccg
     4681 agccggaccg ttcgaggatg accagcgcgt tgcgaaagcc gatcgggctc gcccggcgct
     4741 cgacggcgat ggagcgcccg gcgccgatcg ccgcggcgtc cgagattcgg ccgcccgcat
     4801 gggcgatcgg gagggtccgc gcccgcgacc cggcgacccg cagcacgtgg ttgccttctt
     4861 cgtggaccag aagcagcgcg ccgccctccg cgaccgcgcc ctcgatgccg cgattggcgc
     4921 gccagccgcg cttgccgagg tcgatccgcc ggagcgcccg gcggaagccc gggtcgtaga
     4981 gccacaattg gtggcggttc tcgaacgcga cccaccagcc gcggccgagc ggatcgcgga
     5041 ccagcgcctc ggaatcgcgg tagcgcttga agcgggcgtc gccaggcccg ccgggcagct
     5101 cgccgatgat cgccatttgc cggcgcgggc tgaaccggac cagcgcgccc gagtcggtca
     5161 gcgccagcag ccggccgcgg tcgattgcca gcgccgagac gccgccgaag cgcgggtccg
     5221 cgctggtcaa ctcccacgct ccggccagcc gcagcggagc gaatgcgcgc gagtccagcc
     5281 gcagcggccg ggcctcgatc cgcgcggctc gccagccgaa ctccgcgcgg ttgggaatgc
     5341 gcgccagcgc gaggtcccac agggcgatcg ccgcaagcag gaggagcccc gcggcgagct
     5401 cgcgaatcct gaacttcggc tgcacttccc gttcagggtt cattggctgt gaatcggaca
     5461 taagcccctc aaggcacggg gttttcccct ttccccgtcc cgatttcgat tccctcgcgt
     5521 cgcgtaacga ggggggcccg gtgcgagaaa ttgcgccggg cccatttttt tgtccgcgct
     5581 gggctcagcc ggcgtcgaac agatcggcca gctgctcgat catcgccccg gccaattggt
     5641 cggcgtccat gatcgtcacc gcccgctcgt aatagcgggt gacgtcgtgg ccgatgccga
     5701 tggcgatcaa ttcgaccggg gagcgccgct cgatccactc gatcacctgc cgcaggtggc
     5761 gctcgagata ggtgccgccg ttggccgagg cggtcgaatc gtcgaccggc gcgccgtcgg
     5821 aaatcaccat caggatgcgc cgctcctcgg gccgcgcgat cagccggtgg tgcgcccaca
     5881 gcaaggcctc gccgtcgata ttctccttga gcaggccctc gcgcatcatc agcccgaggt
     5941 tgcgccgcgc gtgccggtag ggctcgtcgg cgcgcttgta gatgatgtgc cgaaggtcgt
     6001 tgaggcggcc cgggtgcccc ggccgccctt cggccagcca cgcctcgcgg ctctggccgc
     6061 ccttccacgc gcgggtggtg aagccgagca cctcggtcgc gaccccgcag cgctcgagag
     6121 tccgggcgag gatgtcggcg cagatcgcgg cgatggcgat cggccgcccg cgcatcgagc
     6181 ccgaattgtc gatcagcacg gaaacgatgg tgtcgcgaaa ctccatgtcg cgctcgacct
     6241 tgtagctcag cgaatgggcc gggttgacga tcacccgcgc cagccgcgcc gaatcgagca
     6301 gcccctcctc ctggtcgaac tcccagctgc gcgcctgctg cgccatcagc cgccgctgca
     6361 gccggttggc gagccgggtg acgacgctct gcgagctcgc catctgctgg tcgagatagg
     6421 cgcgcagccg ccccagttcc tcgacgtcgc acagatcctc ggcctcgacc acctcgtcga
     6481 agcgggtggt gaaggcgcgg tagtcggtct ccggcgcgag ctcccagttg cggcggccgg
     6541 ccgcggcctg cgcgctctcg cttatgtcgt cgtcgcgcga cccgtcctgg tctccgagat
     6601 ccacgtcgtc ggagccctgc tcctggtcgt cgccctcgtc ggcctcctcg ccgcgccgct
     6661 ccatgtcgcc gcccgacggc gagccttcgt cgcc
//
LOCUS       DASTZO010000068         4154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_893340,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000068 DASTZO010000000
VERSION     DASTZO010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893340"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /gene="ubiB"
                     /locus_tag="VFU20_01790"
     CDS             complement(<1..1444)
                     /gene="ubiB"
                     /locus_tag="VFU20_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-polyprenylphenol 6-hydroxylase"
                     /protein_id="HEU5285225.1"
                     /translation="MTASVTHLWRLLKWGRTLARHGALQGIEADPLTPPQVRRLARIA
                     RFAAKVPPTPDYARALQEIGPAAIKLGQALSTRPDLVGDRAAENLSQLQDNLPPAPFA
                     AIKAAIEASFETPLEQLYSSFDPEPIGSASIAQVHRAVTTDGRDVAVKVLRPGIEQEF
                     AQAIETYEWAAAHVESGGGEFERLRPRLVVAHFKQWTARELDLQREAASASELRENMI
                     AEPGFFVPEIDWRRTARRVLTLEWLDGVKLNDRDALVAAGHDCKALAAILVRAFLRQA
                     VVDGFFHADLHQGNLFALADGRIAVIDFGIMGRIDRQARMWLAEILYGLITGNYRRVA
                     EIHFEAQYVPPHHSVEEFATALRAAGEPIRGLPVKDISVGRMLESLFAITRDFDMPTQ
                     PHLLLLQKTMVMEEGVATNLDPDINMWETAEPFLKEWLRSELGPEAFYADKIVDIVRS
                     LKKLPHLIDKIDAQYPEPGAAPPPPPIAEIA"
     gene            complement(1441..2172)
                     /locus_tag="VFU20_01795"
     CDS             complement(1441..2172)
                     /locus_tag="VFU20_01795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5285226.1"
                     /translation="MTSKVNFGDQLVEPDEKTRRVGAVFSSVAHRYDLMNDVMSGGLH
                     RLWKDRFVARVKPRPGERILDMAGGTGDVAFRMARRGARVTVADINPDMLEVGRERAR
                     RRGIDGLEWRVEDAQSLSFEDRSFDAYTIAFGIRNVTDIGAALREAWRVLARGGRFYC
                     LEFSTSDWPGFADAYEAYADNVIPRLGRAVAGDEASYRYLVESIRRFPRPDEFRQMIA
                     DSGFVRAGVEPMLGGLVAIHSGWKI"
     gene            2219..3031
                     /gene="mutM"
                     /locus_tag="VFU20_01800"
     CDS             2219..3031
                     /gene="mutM"
                     /locus_tag="VFU20_01800"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012051058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HEU5285227.1"
                     /translation="MPELPEVETTVRGLARVLEGRRLARVEARRLDLRRAFPQDLGQR
                     LTGATVTDVGRRAKFGLIGTDRGDTLVFHLGMSGHWRIDPDELGRHDHLLLTTDEGRR
                     LALNDARRFGSIDLVATDALDGWQPFAALGPDALGPDLDARWFRRRFEGRRAPVKALL
                     LDQRIIAGLGNIYACEALHRAGIHPARAAGSISLGRLERLAAAIPQVLGEAVAAGGSS
                     LRDFVSPDGTLGYFSARFDVYDREGKKCACGGRVKRIVQGGRSTFYCPRCQR"
     gene            3252..3515
                     /gene="rpsT"
                     /locus_tag="VFU20_01805"
     CDS             3252..3515
                     /gene="rpsT"
                     /locus_tag="VFU20_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU5285228.1"
                     /translation="MANTPQAKKRIRRNAARAAINGARVGRIRTFIKAAESALESGKK
                     DDAAKALKAAQPELARGVARGVIHKNTAARKFSRLSKRLASLG"
     gene            3889..>4154
                     /locus_tag="VFU20_01810"
     CDS             3889..>4154
                     /locus_tag="VFU20_01810"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HEU5285229.1"
                     /translation="MQGGLSTSTLEYSGAPIAAPLEAAWDSIRAGLRRDLGARTFDGW
                     LKPAVLGAFEPDSGALEILMPSQFMADWVRSHFGERLTLAWKTV"
BASE COUNT          627 a         1433 c         1430 g          664 t
ORIGIN      
        1 gcgcgatttc ggcgatcggc ggcgggggcg gggccgcgcc cggctccgga tattgcgcgt
       61 cgatcttgtc gatcaggtgc ggcagcttct tgagcgatcg cacgatgtcg acgatcttgt
      121 cggcgtaaaa ggcctcgggc ccgagctcgg aacgcagcca ttccttcagg aacggctcgg
      181 cggtctccca catgttgatg tcgggatcga ggttggtcgc gaccccttcc tccatcacca
      241 tggtcttctg cagcagcagc aaatgcggtt gggtcggcat gtcgaaatcg cgggtgatcg
      301 cgaacaggct ctcgagcatc cggccgaccg agatgtcctt gaccgggagc ccgcggatcg
      361 gctcgccggc ggcccgcaga gcggtcgcga actcctcgac gctgtggtgc ggcggcacat
      421 attgcgcctc gaaatggatt tcggcgacgc gccggtaatt gccggtgatc agcccgtaga
      481 ggatttcggc cagccacatc cgcgcctggc ggtcgatccg ccccatgatg ccgaagtcga
      541 tcaccgcgat tcgcccgtcg gcaagcgcga acagattgcc ctggtggagg tcggcgtgga
      601 aaaagccgtc gaccaccgcc tggcgcagga aggcgcggac caggatcgcg gcgagcgcct
      661 tgcagtcgtg gccggcggcg accagcgcgt cgcggtcgtt cagtttcacc ccgtcgagcc
      721 actcgagcgt cagcacccgg cgggcggtcc gtcgccagtc gatctcgggc acgaagaagc
      781 ccggctcggc aatcatgttc tcgcgcagct cggaggccga ggcggcctcg cgctgaaggt
      841 cgagctcgcg cgccgtccat tgcttgaaat gggcgacgac cagcctcggg cgcagccgct
      901 cgaactcgcc gccgccgctc tcgacgtggg cggccgccca ttcgtaggtc tcgatcgcct
      961 gcgcgaattc ctgctcgatg cccggccgga gcaccttgac cgcgacgtcg cggccgtcgg
     1021 tggtgaccgc ccggtggacc tgggcgatcg acgccgagcc gatcggctcg ggatcgaagc
     1081 tcgagtagag ttgctcgagc ggggtctcga acgacgcctc gatcgccgcc ttgatcgccg
     1141 cgaacggggc gggcggcaga ttgtcctgca gctgcgacag attctccgcg gctcggtcgc
     1201 cgaccaggtc ggggcgggtc gacagcgcct ggccgagctt gatcgccgcc ggcccgatct
     1261 cctgcagcgc cctggcgtaa tcgggggtcg gcggcacctt ggcggcgaag cgcgcgatcc
     1321 gcgccagccg ccgcacctgc ggcggggtca gcgggtccgc ctcgatcccc tgcagcgcgc
     1381 cgtggcgggc gagcgtccgc ccccatttga ggaggcgcca cagatgggtg accgaagcgg
     1441 tcaaatcttc cagccgctgt ggatggcgac caggccgccg agcatcggct cgacgcccgc
     1501 tcggacgaag ccggagtcgg cgatcatctg ccggaactcg tcggggcgcg ggaagcggcg
     1561 gatcgattcg acgagatagc ggtagctcgc ctcgtcgccg gcgaccgcgc ggccgaggcg
     1621 cgggatgacg ttgtcggcat aagcttcgta ggcgtcggcg aacccgggcc agtcgctggt
     1681 cgagaattcg aggcagtaga agcgcccgcc gcgtgccagc acccgccagg cttcgcgaag
     1741 cgccgcgccg atgtcggtga cgttccggat cccgaacgcg atcgtatagg cgtcgaagct
     1801 gcggtcctcg aagctcaggc tttgggcgtc ctcgacccgc cattcgagcc cgtcgatgcc
     1861 gcgacggcgc gcgcgctcgc ggccgacctc cagcatgtcc gggttgatgt cggcgaccgt
     1921 caccctggcg ccgcgccggg ccatccggaa ggcgacgtcg cccgtgcccc cggccatgtc
     1981 gaggatgcgc tcgcccggcc gcggcttcac ccgggcgacg aagcggtcct tccacagccg
     2041 gtgaagcccg cccgacatca catcgttcat caggtcgtag cggtgcgcga ccgagctgaa
     2101 aaccgcgccg acgcggcggg tcttctcgtc gggctcgacc agctggtcgc cgaaattgac
     2161 cttggacgtc atggcggtgc ctctagcggg cttggcgggc cttcgccata tcgcggccat
     2221 gcccgagctc cccgaagtcg agaccaccgt ccgcggcctg gcgcgcgtgc tcgagggccg
     2281 gcggctggcc cgcgtcgagg cgcgtcggct ggatctgcgc cgggcgtttc cgcaggatct
     2341 cggccagcgg ctgaccggcg cgacggtgac cgatgtcggc cgccgcgcga agttcgggct
     2401 gatcggcacc gaccgcggcg acacgctcgt cttccacctc ggcatgtccg ggcactggcg
     2461 gatcgacccg gacgagctcg gccggcacga ccatttgctg ctgacgaccg acgagggccg
     2521 gcggctggcg ctcaacgacg cgcgccgctt cggctcgatc gacctggtcg cgaccgacgc
     2581 gctcgacggg tggcagccgt tcgccgcgct ggggcccgac gccctcggcc ccgatctcga
     2641 cgcgcgctgg ttcaggcgcc gcttcgaggg ccgccgggcg ccggtcaagg ctcttctcct
     2701 cgaccagcga atcatcgccg ggctaggcaa catctacgcc tgcgaggcgc ttcaccgggc
     2761 gggcatccac ccggcgcgcg ccgccggctc gatctcgctg gggcgcctcg agcggctggc
     2821 ggcggcgatc ccgcaggtgc tcggcgaggc ggtcgccgcc ggcggctcga gcctgcgcga
     2881 tttcgtcagc cccgacggga ccctcggcta tttttcggcc cggttcgacg tctacgaccg
     2941 cgagggcaaa aagtgcgcgt gcggcgggcg ggtcaagcga atcgtccagg gcggccgctc
     3001 gaccttttac tgcccgcgct gccagcggtg aggtatcgcg ctcgatccgg caccggcccg
     3061 cacccccacc cggccaccca tcgaggatat tctgcatggg tggccgggtg ggggcgcggg
     3121 ccggcacggc gcttcgcagc aacggcgtcg gttgacccga atcgcccttg ccggtagggg
     3181 agcgcgacgc cggtgcggcc ctcgggcgcg cgccgctttt cttttcgatc tgacgaaccg
     3241 aggacttttc gatggcgaac acgccgcaag ccaagaagcg catccgccgc aacgccgctc
     3301 gcgccgcgat caacggcgcc cgcgtgggcc ggatccggac cttcatcaag gccgccgagt
     3361 cggcgctcga gtcgggcaag aaggacgatg ccgccaaggc gctgaaggcg gcccagccgg
     3421 agctggcgcg aggagtcgcg cgcggcgtca ttcacaagaa caccgccgcc cgcaaatttt
     3481 cgcggctgtc caaaaggctt gcttccctgg gctgaatcga ggcctcaaaa accgcctcca
     3541 aattgcgacc ggcccggtcc ccgattggtc ccatttcggg acctttcaaa aaacccagct
     3601 tttcccgcga ttcgcgctcc gggtaaaatc aaagctaaca aaaaagtcgc agttttccgc
     3661 gcctttcccg gctttccgag ggtttttgct gtcaaggctg attatttcac ttttgtgaaa
     3721 aaacaggtct tgaaagctcg cccggacgct tccagaaaca gccttccggg cgccgagtct
     3781 gcgtcagggg gctagagaga aaaaaagcca tcggcgccgc gaatcaaatc gggcgcagcg
     3841 gccccttttt gtctctgtcc gccactgtga gggaggcgag aagggcaagt gcaaggcggt
     3901 ctgtccactt cgaccctgga gtactccggc gcaccgatcg cagcgccact cgaagccgcc
     3961 tgggattcga tccgcgccgg ccttcgccgc gaccttggcg cacggacgtt cgacggctgg
     4021 ctcaagcccg cggtcctcgg tgcgttcgag ccggacagcg gagcgctcga aatcctgatg
     4081 ccgagccagt tcatggccga ctgggtccgc tcccatttcg gcgagcggct gacgctggcg
     4141 tggaagaccg tctt
//
LOCUS       DASTZO010000069         3987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_893487,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000069 DASTZO010000000
VERSION     DASTZO010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893487"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1406)
                     /locus_tag="VFU20_01815"
     CDS             complement(<1..1406)
                     /locus_tag="VFU20_01815"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEU5285230.1"
                     /translation="MTLNATVARVTDRIIERSKPKRRRYLELIADQKERGISRPRMSC
                     GNFAHGFAAAGDDKQPIRTLAGPNIGIVTAFNDMLSAHQPYGRYPEQIKIFAREAGAT
                     AQVAGGVPAMCDGVTQGQPGMDLSLFSRDVIAMSTAVALSHGMFDAAALLGICDKIVP
                     GLLIGALRFGHLPALLIPGGPMPSGLPNKEKQRVRQLFAEGKVGRDELLEAEAKSYHS
                     AGTCTFYGTANSNQMMMELMGLHIPNAAFINPNTPLRQALTRAAVHRLAELGRERIRP
                     IGEVVDEKAIVNAMVGLLATGGSTNHAIHLPAIARAAGITVDWEDFAGLSAAVPLIAR
                     IYPNGARDVNDFHAAGGMAFVAATLLSQGLIHADTLTAGADDFSAYTASPAIEGNALV
                     WKAAGDSLDLDMLRPVTDPFLPDGGMKLVAGNLGRAIFKTSSVEADRWTIEAPARCFS
                     DQNAVLEAFKAGELDRDVA"
     gene            complement(1437..2894)
                     /gene="zwf"
                     /locus_tag="VFU20_01820"
     CDS             complement(1437..2894)
                     /gene="zwf"
                     /locus_tag="VFU20_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEU5285231.1"
                     /translation="MADKVSALLLFGATGDLARRMLLPSLYGLDADGLLPDELVVIGT
                     ARSELDDDAYRARARQALDEHLPAGFFNSEVGERFLKRLRYVPLDIGDESGFERLAEA
                     VGDPERGLAIFLSTAPSLFKPTIDGLAAAGLACPTVRMALEKPLGSDLTSSREINDSV
                     AAAFPEERTFRIDHYLGKETVQNLLALRFANSLFEPLWNSTHIDHVQISVAETVGIEG
                     RGDYYDGAGALRDMVQNHMLQLLALVAMEPPTNFDATAVRDEKVKVLRSLRRPAADTI
                     AAHIVTGQYGRGAIDGETVPGYAEELGRDSSTETFVALKAYVDNWRWKGVPFYLRTGK
                     RLPERQTEIYIQFNGVPHSIFAARGATTSPNKLVIGLQPEETIRLLMMAKTPGLDRQG
                     IRLREVPLDLGLADAFADRRRRIAYERLLLDLIEGDPTLFVRRDEVEAQWEWIDAIRA
                     AWGEAGVAPRPYAAGTWGPSAAIALTERDGVSWHE"
     gene            complement(2884..3816)
                     /locus_tag="VFU20_01825"
     CDS             complement(2884..3816)
                     /locus_tag="VFU20_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU5285232.1"
                     /translation="MILRLAALLAIAAVPAAAGISAGRLDDLGVVQSRHADPRKVTVW
                     LPSSYRPGGPRHSVLYMHDGQNLFDPETGYGGMEWTIDEVLDRLIRENKVRPTIVVAI
                     WNTPKRLREYVPSKAFRHLSPAYMERVRGLYGGDPLSDGYLQFIVRELKPRIDRTYRV
                     RTGRADTAIMGSSMGALISLYAINEYPRVFGAAGMVSTHWPLMIPPKGQSLSDAEYET
                     VSSAFERYLAPALPSPRTHRLYFDHGSETLDAVYARYQRRIDRVVARRGYRPGANWVS
                     RNFPGQAHNETSWASRVEIPLRFLLPPRKGAHGR"
BASE COUNT          599 a         1359 c         1396 g          633 t
ORIGIN      
        1 gcgacgtcgc ggtcgagctc gccggccttg aacgcctcga gcactgcgtt ctggtcggaa
       61 aagcagcgcg ccggcgcctc gatggtccag cgatcggcct cgaccgagct ggtcttgaag
      121 atcgcccggc ccaaattgcc cgcgaccagc ttcattccgc cgtcgggcag gaacgggtca
      181 gtgaccgggc gcagcatgtc gaggtcgagg ctgtcgcccg cggctttcca caccagcgca
      241 ttgccctcga tcgccgggct cgccgtgtag gcggaaaaat cgtcggcgcc ggcggtcagg
      301 gtgtcggcgt ggatcaggcc ctgcgacaga agcgtggcgg cgacaaacgc catcccgccg
      361 gcggcgtgga agtcgttcac gtcgcgcgct ccgttgggat agattcgcgc gatcagcggc
      421 acggcggccg acaggccggc gaaatcctcc cagtcgaccg tgatccccgc ggcgcgggcg
      481 atcgccggca gatggatcgc atggttggtc gagccgccgg ttgccagcag cccgaccatc
      541 gcgttgacga tggctttctc gtcgaccacc tcgccgatcg gccggatgcg ctcgcgcccc
      601 agttccgcca gccggtggac agcggcgcgg gtcagcgcct gcctgagcgg cgtgttcggg
      661 ttgatgaacg ccgcattggg gatgtgcagc cccatcagct ccatcatcat ctggttggag
      721 ttggcggtgc cgtagaaggt gcaggtgccg gcgctgtggt acgacttggc ctcggcctcg
      781 agcagctcgt cgcggccgac cttgccctcg gcgaacagct ggcggacgcg ctgcttctcc
      841 ttgttcggca ggcccgacgg catcggccca ccggggatca gcaacgccgg cagatggccg
      901 aagcgcagcg ccccgatcag caggccgggc acgatcttgt cgcagatgcc gagcagggcc
      961 gccgcgtcga acatgccgtg gctcaatgcg accgcggtgc tcatcgcgat cacgtcgcgg
     1021 ctgaacagcg acaggtccat gcccggctgg ccctgggtca ccccgtcgca catcgccggc
     1081 accccgccgg cgacctgcgc ggtggccccc gcctcgcggg cgaatatctt gatctgctcg
     1141 ggatagcgcc cgtacggctg gtgcgccgag agcatgtcgt tgaatgcggt gacgatgccg
     1201 atgttgggcc cggccagagt gcggatcggc tgcttgtcgt cgccggcggc agcgaagccg
     1261 tgggcgaaat tgccgcacga catccgcggc cggctgatgc cgcgctcctt ctggtcggcg
     1321 atcagctcga ggtagcgccg ccgcttgggc ttcgagcgct cgatgatccg gtcggtgacc
     1381 cgggccacgg ttgcgttgag cgtcattgtt cgactcctgc ggcggtccgc cgccgtttac
     1441 tcgtgccagc tgacgccgtc gcgctccgtc agcgcgatcg ccgccgatgg cccccaggtg
     1501 ccggccgcat aaggccgcgg tgcgaccccg gcctcccccc aggcggcgcg gatcgcgtcg
     1561 atccattccc actgcgcctc gacctcgtcg cggcgcacga acagcgtcgg gtcgccctcg
     1621 atcaggtcga gcagcaaccg ctcgtaggcg atccgccgcc gccggtcggc gaacgcatcg
     1681 gcgaggccga ggtcgagcgg cacctcgcgc agccggatcc cctggcggtc gagccccgga
     1741 gtcttggcca tcatcagcag gcggatggtc tcctcgggct ggaggccgat gaccagcttg
     1801 tttggactgg tcgttgcgcc ccgcgcggcg aagatcgagt gcggcacgcc gttgaactgg
     1861 atgtagatct cggtctggcg ctcgggcagc ctcttgccgg tgcgcagata gaaaggcacg
     1921 cccttccacc gccaattgtc gacataggcc ttcaaggcga cgaacgtctc ggtcgagctg
     1981 tcgcggccga gctcctcggc atagccaggc acggtctcgc cgtcgatcgc cccgcggccg
     2041 tactggccgg tgacgatgtg ggccgcgatg gtgtcggcgg ccggccggcg aagcgaacgc
     2101 agcaccttga ccttctcgtc gcgcaccgcg gtcgcgtcga agttggtcgg cggctccatc
     2161 gccaccagcg ccagcaattg cagcatgtgg ttctggacca tgtcgcgaag cgcaccggcg
     2221 ccgtcgtaat aatcgccgcg gccctcgatc ccgaccgtct cggcgacgct gatctggaca
     2281 tggtcgatgt gggtgctgtt ccacagcggc tcgaacagcg agttggcgaa gcgcagggca
     2341 agcaggttct ggaccgtttc cttgccgaga taatgatcga tccggaacgt gcgctcctcg
     2401 gggaaggcgg cggcgaccga atcgttgatc tcgcggctcg acgtcaggtc cgaccccagc
     2461 ggcttctcga gcgccatgcg cacggtcggg caggcgaggc ccgcggccgc gagcccgtcg
     2521 atggtcggct tgaacagcga cggcgcggtc gacaggaaga tcgccagccc gcgctccggg
     2581 tcgccgaccg cctcggcgag ccgctcgaat ccggactcgt cgccgatgtc gagcggcacg
     2641 tagcgcagcc gcttgaggaa tcgctcgccg acctcggaat tgaagaatcc ggcgggaaga
     2701 tgctcgtcga gcgcctgcct tgccctggcc cggtaggcgt cgtcgtcgag ctcgctgcgg
     2761 gcggtgccga tgacgaccag ctcgtcgggc agcagcccgt cggcgtcgag cccgtagagc
     2821 gacggcaaca gcatccggcg cgccaggtcg ccggtcgctc cgaacagcag caatgcggaa
     2881 accttatcgg ccatgcgccc ccttacgcgg cggcagcagg aaacgcagcg ggatttcgac
     2941 ccgcgacgcc cagctggtct cgttgtgcgc ctggccgggg aagttgcggc tgacccagtt
     3001 ggcgcccgga cgatagccgc gcctcgcgac cacccggtcg atccgccgct ggtagcgggc
     3061 gtagaccgcg tcgagggtct cgctgccgtg gtcgaaatag aggcggtgcg tgcgcggcga
     3121 cggcagagcg ggcgcgaggt agcgctcgaa cgccgacgac accgtctcat attccgcgtc
     3181 gctcagcgac tggcccttgg gcgggatcat cagcggccag tgggtcgaaa ccatccccgc
     3241 cgccccgaac acgcgcggat attcgttgat cgcgtagagc gagatcagcg cccccatcga
     3301 cgagcccatg atcgcggtgt cggcgcggcc ggtgcgcaca cggtatgtcc ggtcgatgcg
     3361 cggcttgagc tcgcggacga tgaattgcag atagccgtcg gacaatgggt cgccgccgta
     3421 gaggccgcgg acccgctcca tgtaggccgg ggataagtgc cggaacgcct tcgacggcac
     3481 atattcgcgc aggcgcttgg gcgtgttcca gatcgcgacg acgatcgtcg ggcggacctt
     3541 gttctcgcgg atcagccggt cgagcacctc gtcgattgtc cactccatgc cgccatagcc
     3601 ggtctcggga tcgaacaggt tctggccgtc gtgcatataa agcaccgagt gcctcggccc
     3661 gcccggccgg tagctcgatg gcagccagac cgtgaccttg cgcgggtcgg cgtggcgcga
     3721 ctggacgacg ccgaggtcgt cgagccgtcc ggccgagatg ccggccgccg ccggcacggc
     3781 cgcgatcgcc agcaaggccg caagccggag gatcatgcgg cggcctcgag caatccgagc
     3841 gcctcggcgt ggcgcgggaa ggccttcacg ctctcggcga tgaagcggtc gatctcgtcc
     3901 atgtcgaccc ggacgacgtc gggcccgggc gggcccagct tcgccgcgtc ggggaaattg
     3961 ccgtagccgg agagcatgca gtgccac
//
LOCUS       DASTZO010000070         5634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1258739,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000070 DASTZO010000000
VERSION     DASTZO010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5634
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1258739"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..492)
                     /locus_tag="VFU20_01830"
     CDS             complement(<1..492)
                     /locus_tag="VFU20_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB"
                     /protein_id="HEU5285233.1"
                     /translation="MAEEALPAETTVRRERRLRSDWPRRLAQELLALVVSLLILLAAG
                     LVLLDTAPGHRFIVDRIARIETASGLNIRIGRIDGSVFGKSKLKNVTVSDTRGVFLTS
                     GEIELDWAPGAWLYNSLHIDRLYARQVRLTRLPKLRPTGRKGPILPGFDIHIGELVID
                     RLEL"
     gene            complement(494..2470)
                     /locus_tag="VFU20_01835"
     CDS             complement(494..2470)
                     /locus_tag="VFU20_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU5285234.1"
                     /translation="MAARLWCAAALALAPAAPGLAQPAPPGGPAELDPSAPLDPMPDL
                     GLDWPDPGAADEPAAEPDPQHIAQEPEAPPPVSDGAAELRYAIAFEGLEKIGPTDELI
                     ASFEAQSVLHKERGDAANAAQVDRRSRADAELLAELLRSHGYYDAIVEPRIEWTGGTL
                     LVVLEASPGEQYRFQSVELPGLEAAGAEAASLREAFAVKAGDPVVAEDVIAANVALKV
                     ALGEQGFALAEVGEQEILIDHETRRARLVLPVRTGPLASFGVLRVSGRPPFSPAHVAR
                     IARFEPGQQFRRSKVDDLRRALIATGLVASAEVRVVPAADGRTVDLAVHLDPAPMRTI
                     AGELGYGTGEGVRAEASWQHRNLFNPEGALTVRGVAGTQEQLAAVQFRRNNFMARDQV
                     LNLHALASHTDRDAYEAKTLLIGGNIERQSNIIWHKKWTWNVGADLLATDERGVFDSL
                     GMKDTKTFLIAAFPANLGYDGSDDLLDPTRGFRLGGRISPEISAHGGKFAYARAQLDG
                     SAYRPVSPGVVAAGRVRAATIFNAEAMSIAPSRRLYAGGGGSVRGYGYQKLGPRDLDG
                     DPIGGRALAEFALEARVRLKAFGGNFGVVPFLDGGTLTNDSWPRFSGWQFGAGLGVRY
                     YSSFGPIRIDVGTPLNPREGDGPVAVTVSLGQAF"
     gene            complement(2611..2820)
                     /locus_tag="VFU20_01840"
     CDS             complement(2611..2820)
                     /locus_tag="VFU20_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285235.1"
                     /translation="MDSPFISGRAALADAAELLERFGAHAGTEAAARADTSRNAGNVS
                     RFCHWRQIERVIVALGSDEVRGTVH"
     gene            2941..3108
                     /locus_tag="VFU20_01845"
     CDS             2941..3108
                     /locus_tag="VFU20_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285236.1"
                     /translation="MARRNARAGGIFLTLGILAGFGWGIAAGDPMKGILTGTGLGAAA
                     ALLLWLLDRRR"
     gene            complement(3113..3757)
                     /locus_tag="VFU20_01850"
     CDS             complement(3113..3757)
                     /locus_tag="VFU20_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HEU5285237.1"
                     /translation="MLSEPRLVIERLCAERGEDFAGLSRMLGRNPAYIQQFVRRGVPK
                     RLKEAERRKLARYFAISESLLGGPAEESGPGGLVPVKRIAVRASAGPGALVGGEAAKP
                     YFGFDGRWLKALTSTAPGKLSIIRVEGDSMAPTLNAGDDILVDPGDCTDRLRDGIYVM
                     RVDDALVVKRIALHPVGHRVTVQSDNPAYPDWPDCDMEEIHCIGRVIWAGRRID"
     gene            4038..4370
                     /locus_tag="VFU20_01855"
     CDS             4038..4370
                     /locus_tag="VFU20_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285238.1"
                     /translation="MLNMVMSRAASGLLRALLDRAGDQRDRILLSEIRSVDWQSLTLA
                     GERHLVTLRVVGADAQTLVDGFVSGIEDAEFAIPGHIVADIAVAGIPTRELDLSVTLE
                     IEALTVLE"
     gene            complement(4374..4796)
                     /locus_tag="VFU20_01860"
     CDS             complement(4374..4796)
                     /locus_tag="VFU20_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285239.1"
                     /translation="MEWASEDNPVEQAVDVAAAALFAAAVGFAAGAAGLGAGATIFAA
                     TATSLPVYAVLKSVAAGERVYALPAFEPAPLELASHSDDDELLLEDRLGIVTPDARVV
                     CLFGPGQRSGGCASSSSALPDASQALTAALAELRRSLR"
     gene            complement(4833..5336)
                     /locus_tag="VFU20_01865"
     CDS             complement(4833..5336)
                     /locus_tag="VFU20_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007677013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEU5285240.1"
                     /translation="MATDGFEESELFGPLEILTRRGAEVRLAAPTPSPIQATVRDDPG
                     KVIRPDLTIGQADAADFHALLLPGGVRNPDALRTDAEAIALIRAFAEAGKPVAAICHG
                     PWLLVEADLLRGRKATCWRSIRTDLANAGAILLDQPAVTDGNIVTSRMPEDVEAFTEA
                     LIALIER"
     gene            5453..>5634
                     /locus_tag="VFU20_01870"
     CDS             5453..>5634
                     /locus_tag="VFU20_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEU5285241.1"
                     /translation="MLRHSSDCEPGFTRKRMGRYWAYFDAKGERITDRETIERLNAIG
                     LPPAYTNAWFCADSNG"
BASE COUNT          868 a         1966 c         1944 g          856 t
ORIGIN      
        1 gagctcgagc cggtcgatca ccagctcgcc gatgtggatg tcgaagccgg gcaggatcgg
       61 gcccttgcgc cccgtcggcc tcagcttggg cagccgggtc agccgcacct ggcgcgcata
      121 cagccggtcg atgtgaagcg agttgtacag ccacgcgccc ggcgcccagt cgagctcgat
      181 ctcccccgac gtgaggaaga cgccgcgcgt gtcggacacc gtcacattct tcagcttcga
      241 cttgccgaac accgacccgt cgatccggcc gattcggatg ttgagccccg acgcggtctc
      301 gatccgggcg attcggtcga cgatgaagcg gtggcccgga gccgtgtcga gcagcaccag
      361 cccggcggcc agcaggatca gcagcgacac gaccagcgcc agcagctcct gcgcgaggcg
      421 gcggggccag tcgctgcgca ggcgccgctc gcgccgaacc gtcgtctcgg ccggaagagc
      481 ttcctcggcc actctagaac gcctggccga gcgagacggt gaccgcgacc gggccgtcgc
      541 cctcgcgcgg gttcaacggc gtgccgacgt cgatccggat cggcccgaag ctcgaataat
      601 agcggacgcc aagcccggcg ccgaactgcc agccgctgaa cctgggccag ctgtcgttgg
      661 tcagggtgcc gccgtcgagg aacggaacga caccgaaatt gcctccgaac gccttcagtc
      721 ggacgcgcgc ctcgagcgcg aactcggcaa gtgcgcggcc gccgatcggg tcgccgtcga
      781 ggtcgcgggg cccgagcttc tggtagccgt agccgcggac cgatccgccg ccgcccgcgt
      841 acagccgccg cgacggggcg atcgacatgg cttcggcatt gaagatggtc gcggcgcgca
      901 cccggccggc ggcgacgacg cccggcgaca ccggccggta ggcgctgccg tcgagctggg
      961 cgcgcgcgta ggcgaacttg ccgccgtggg ccgagatttc ggggctgatc cgcccgccga
     1021 gccggaaccc cctggtcggg tcgagcaaat cgtcgctgcc gtcgtagccg aggttggccg
     1081 ggaaggcggc gatcaggaag gtcttggtat ccttcattcc gagggaatcg aagactccgc
     1141 gctcgtcggt cgcgagcagg tcggcgccga cgttccacgt ccatttcttg tgccagatga
     1201 tgttgctctg ccgctcgatg ttgccgccga tcagcagggt cttggcctca taggcgtcgc
     1261 ggtcggtgtg gctggccagg gcgtgcaggt tgagcacctg gtcgcgcgcc atgaaattgt
     1321 tgcggcggaa ctggaccgcg gcgagctgct cctgggttcc cgcgaccccg cggacggtca
     1381 gcgccccttc ggggttgaac aggttgcggt gctgccagct ggcctccgcg cggacgccct
     1441 cgccggtgcc gtagccgagc tcgccggcga tggtgcgcat cggcgccggg tcgaggtgga
     1501 cggcaaggtc gacggttcgc ccgtcggcgg ccgggactac cctgacctcg gcggaggcga
     1561 ccagcccggt ggcgatcagg gcccggcgga ggtcgtcgac cttcgagcgg cggaactgct
     1621 ggccgggctc aaagcgggcg atgcgcgcga cgtgggccgg gctgaacggc ggccggccgc
     1681 tgacccggag cactccgaac gacgccagcg ggccggtcct gaccggcagc accagccgcg
     1741 cccggcgggt ctcgtggtcg atcaggattt cctgctcgcc gacctcggcc agagcgaagc
     1801 cctgctcgcc gagcgcgacc ttcaaggcga cattggccgc gatcacgtcc tcggcgacga
     1861 ccgggtcgcc ggccttgacc gcgaacgcct cgcgcagcga cgccgcctcg gcgcccgccg
     1921 cttcgaggcc gggcagctcg accgactgga accggtattg ctcgcccgga ctggcttcga
     1981 gcacgaccag cagcgttccg ccggtccact cgatccgcgg ctcgacgatc gcatcgtaat
     2041 agccgtggct gcgcaatagc tcggcgagga gctcggcatc ggcgcgcgag cggcggtcga
     2101 cctgggccgc gttggcggcg tcgccgcgct ccttgtgaag caccgactgc gcttcgaacg
     2161 aggcgatcag ctcatcggtc ggcccgatct tctccaggcc ttcgaacgca atcgcatagc
     2221 gaagctcggc ggctccgtcg gagaccggcg gcggcgcctc cggctcctga gcgatgtgct
     2281 gcgggtccgg ctcagctgcg ggctcatcgg cggcgccggg gtccggccag tcgaggccga
     2341 ggtcgggcat cgggtccagt ggggccgagg gatcgagctc ggccgggccg cccggaggcg
     2401 cgggttgcgc gaggcccgga gcggcgggcg ccagcgccag cgcggccgcg caccacagcc
     2461 gcgccgccac ccgcgagcga caggctccaa tcgcccgcaa gcgattggct cgcctgttca
     2521 ctccccgccg gttcatcgcg ccagcctagc cagcactcct gaaccgcgcc agtatgaacg
     2581 ccccaagccc ttgattcagc ggccgtccaa tcagtggacc gtgccgcgca cctcgtcgct
     2641 gccgagcgcc acgatcaccc gctcgatctg gcgccagtgg caaaagcggc tgacgttgcc
     2701 ggcgttgcgg ctggtgtccg cgcgcgccgc ggcctcggtg ccggcgtgcg cgccgaagcg
     2761 ctcgagaagc tcggccgcgt cggccagcgc cgctcgcccg ctgatgaacg gtgaatccat
     2821 ggaggcggcc ttaaaccggc gcttgttacg cgactgccga caaaggtcgt caacgcggcg
     2881 ttgaccacag gagcgcactt tggggctgga gcggccgggg cccgtctgcc atggcgccgc
     2941 atggccagac gcaacgcccg cgccggcggc attttcctca ccctgggcat cctcgccggc
     3001 ttcggctggg gaatcgccgc cggcgacccg atgaagggca tattgaccgg caccgggctg
     3061 ggcgccgcgg cggcgctcct gctgtggctg ctcgaccgcc ggcgctaggc gctcagtcga
     3121 tccgccgccc cgcccagatc actcggccga tgcagtggat ctcctccatg tcgcaatcgg
     3181 gccagtcagg gtaagccgga ttgtcggact ggacggttac ccggtgcccg accgggtgca
     3241 gtgcgatccg cttgacgacc agcgcgtcgt cgacccgcat cacgtagatg ccgtcgcgca
     3301 accggtcggt gcagtcgccc gggtcgacca atatgtcgtc gccggcgttc agggtcgggg
     3361 ccatcgaatc gccctcgacc cggatgatcg acagcttgcc cggggcggtc gaggtcagcg
     3421 ccttcagcca ccgcccgtcg aaaccgaaat agggcttggc ggcctccccg ccgaccagcg
     3481 ctcccggccc tgccgaggcc cggaccgcga tccgcttgac cgggaccagc ccgcccggcc
     3541 cgctttcttc ggccgggccg ccgagcagcg attccgaaat cgcgaaatag cgcgccagct
     3601 tgcggcgctc ggcctccttg aggcgcttgg gcaccccgcg gcggacgaat tgctggatat
     3661 aggcggggtt cctgccgagc atccgcgaca ggccggcgaa gtcctccccc cgctcggcgc
     3721 agaggcgttc gatgacaagc cgcggttctg acaacatccc gttcccttaa ctatcttttc
     3781 ttaattatag gcaactgcct agacaagtag gagatgcgtt gggaaataat atgtagccga
     3841 gtcgctctgg attataagga atgaagagtg cacttgcttc gagagataga aaaatttctg
     3901 cgccgtagcc acacggctcc gacccgtttt ggacgagaag cccttggcga ccctcgtttt
     3961 gttttcgatc tgcggaaagg aagggagccg cgacccggga ccgtccgacg agtccgggcg
     4021 ttcctggagg ccgcgcaatg ttgaacatgg tgatgtcccg ggccgcgtcc gggctgctcc
     4081 gcgccctgct cgaccgcgcc ggagaccagc gtgataggat tttgctcagc gaaatccgtt
     4141 cggtcgactg gcaatcgctt acgcttgccg gcgaacggca tctggtcact ctgcgcgtgg
     4201 ttggcgcgga tgcgcaaacg ctggtcgatg ggttcgtcag cggaatcgag gatgccgaat
     4261 tcgccatccc cggccacatc gtcgccgaca tcgccgtggc cggaatcccg acgcgcgagc
     4321 tggacctgtc cgtgacgttg gaaatcgagg cgctgacggt cctcgagtaa gactcagcgc
     4381 aacgagcggc gcaattcggc cagcgccgcg gtcagcgctt gcgaagcatc gggaagtgcg
     4441 gagctggaag aggcgcaccc tcccgacctc tggcctgggc cgaacagaca cacgacccga
     4501 gcatcgggcg tcacgatccc gagccggtcc tcgagcagaa gctcgtcatc gtcggaatga
     4561 ctggccagct cgagcggcgc cggctcgaag gcgggcaacg cgtagacccg ctcgccggcc
     4621 gcgacgctct tgaggacggc atagaccggc agggacgttg ccgtggccgc gaagatcgtc
     4681 gccccggccc cgagccccgc tgcaccggcc gcaaagccga cggcggccgc gaacagcgcg
     4741 gcggccgcga cgtcgaccgc ctgctcgacg ggattgtcct cgcttgccca ttccatcgcc
     4801 tgcgggctta gcggaaattg gtttccagtc gcttagcgct cgatgagggc gatcaacgcc
     4861 tcggtgaacg cctcgacgtc ctcgggcatg cggctggtga cgatattgcc gtcggtcacc
     4921 gccggctggt cgagcaggat cgcgcccgcg ttcgcaaggt cggtgcggat cgagcgccag
     4981 caggtggcct tgcgcccgcg cagcaggtcg gcctcgacca gcagccacgg cccgtggcag
     5041 atcgccgcga ccggcttgcc ggcctcggcg aaggcgcgga tcagcgcgat ggcctcggca
     5101 tcggtgcgca gcgcgtcggg gttgcgcacc ccgcccggaa gcagcagcgc gtggaaatcc
     5161 gcggcgtccg cttgcccgat cgtgaggtcg ggccggatga ccttaccggg atcgtcgcgc
     5221 accgtcgcct ggatcgggct cggcgtcggc gccgccaggc ggacctcggc cccgcggcgc
     5281 gtcaggattt cgagcgggcc gaacagctcc gattcctcga agccgtcggt cgccatgatc
     5341 agcactctgg aatcaccgat cgccggaatc gccgcctcct cgggccgccc gcaggaacat
     5401 gtgcagccac cactcgctta tgccggccaa tgccgcaaga attgaacgcc tgatgctccg
     5461 ccacagcagc gattgcgaac ccggcttcac ccgcaagcgg atgggccgct actgggccta
     5521 tttcgacgcc aagggcgagc ggatcaccga ccgcgagacg atcgagcggc tgaatgcgat
     5581 cggccttccg ccggcctaca caaacgcctg gttctgcgcc gattccaacg gcca
//
LOCUS       DASTZO010000071         3242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_969806,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000071 DASTZO010000000
VERSION     DASTZO010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969806"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="VFU20_01875"
     CDS             complement(<1..523)
                     /locus_tag="VFU20_01875"
                     /inference="COORDINATES: protein motif:HMM:NF027558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4893 domain-containing protein"
                     /protein_id="HEU5285242.1"
                     /translation="MTRAFALLALGLCLAACSAMRPSGAVIDAPTRDWRAVATNDDRE
                     RLRDWRGAFVSGLKAARGAGHGEAIAREGVLLEPDSALPGGAIPNGDYRCRAIKLGAK
                     SPGLLDYVAYPPFACRIRQERDLQGFAKLTGSQRQVGLIFPGDAMRQVFLGTLVLGDE
                     RRAMQYGRDQERDV"
     gene            complement(520..1287)
                     /locus_tag="VFU20_01880"
     CDS             complement(520..1287)
                     /locus_tag="VFU20_01880"
                     /EC_number="1.1.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003858 - 3-hydroxybutyrate dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyrate dehydrogenase"
                     /protein_id="HEU5285243.1"
                     /translation="MILEGKVALVTGSTSGIGLEIARALAAEGAAVMLNGFGDRQEIE
                     RLCGELGAVHDGADLSDPSAIEAMLDRCSAELGAPDVLVNNAGIQHVSPVESVPPGKW
                     DAIIAINLSAAFHTTRLALPAMRARKWGRIINTASAHSLVASPNKSAYVAAKHGIAGL
                     TKTVALEAARDGVTVNCISPGYVWTSLVENQIPDTMKARGLTREQVMNDVLLAAQPTK
                     RFVTPEEVAALALFLCRDEAASITGANLSMDGGWTAQ"
     gene            1384..2241
                     /locus_tag="VFU20_01885"
     CDS             1384..2241
                     /locus_tag="VFU20_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HEU5285244.1"
                     /translation="MRLGDEDPSGHFIDRTGRGGFGGGGSNLLGCLLPLVASKFGIVG
                     VLVLLLGYCALTSLGGGGLLPGGGTSTTAPSGQSTIDPNTRDLLARVLGSTERRWSEI
                     FARTGAQYQPTKMIAYSNTDQSGCGAAQAAMGPFYCPTDQSIYIDPQFFSELAQRFGA
                     PGDFAMAYVIAHEVGHHVQNLQGTLESAQRAQASASRTEGNQIQVAVELQADCYAGVW
                     AARDQNLLEPGDVEEGMRAAEAIGDDTLQKQMQGRVVPESFTHGTAAQRQAALRRGLE
                     TGDPAACRF"
     gene            complement(2238..3224)
                     /locus_tag="VFU20_01890"
     CDS             complement(2238..3224)
                     /locus_tag="VFU20_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEU5285245.1"
                     /translation="MARAWHLKSRPEGLPTADNFELKPIELPGLGEGMVRVANRWLSV
                     DPYMRGRMNDVKSYVPPFALGAPMEGGALGEVVESRAEGFKPGDLVVHMAGWRDEAVL
                     PAAAVTRLPDLGVEPQAFLGNLGLTGGTAYFGLLDAAQAKEGDLVFVSAAAGAVGSTV
                     VQIAKARGMTVIGSARGAAKCEFVRSLGADAVVDYTAGPVVKSLAAAAQDGIDVYFDN
                     VGGDHLDAALALARRNARFAICGMIGGYNAAEPMCLRYIMRIIAMRIRLQGFIFTDYL
                     PRLAEFYRDMGGWIASGHVKSRETIVEGLERMPDAFLGLFEGANTGKMLVRL"
BASE COUNT          489 a         1138 c         1138 g          477 t
ORIGIN      
        1 cgacgtcgcg ctcctggtcg cggccatatt gcatcgcccg ccgctcgtcg ccaagcacca
       61 gcgtgccgag gaacacctgg cgcatcgcgt cgcccgggaa gatcaggccg acctggcgct
      121 gcgagccggt caatttggcg aatccctgga ggtcgcgctc ctggcggatc cggcaggcga
      181 agggcgggta agcgacataa tcgagcagcc ccggcgattt tgcgccgagc ttgatcgccc
      241 ggcagcggta atcgccattg ggaatcgccc cgccgggaag cgccgaatcg ggctcgagca
      301 gcaccccttc gcgggcgatg gcttcgccat ggccggcgcc gcgcgccgcc ttgagcccgg
      361 agacgaacgc gccgcgccag tcgcgcagcc gctcgcggtc gtcgttcgtc gcgaccgcgc
      421 gccagtcgcg cgtcggcgcg tcgatcaccg cgccgctcgg ccgcatcgcc gagcaggccg
      481 cgaggcacag gccgagggcg agcagggcga aagcgcgcgt cattgcgcgg tccagccgcc
      541 gtccatgctg aggttggcgc cggtgatcga ggcggcctcg tcgcggcaca ggaacagcgc
      601 cagcgccgcc acctcctcgg gggtgacgaa gcgcttggtc ggctgggcgg cgagcagcac
      661 gtcgttcatc acctgctcgc gggtcaggcc gcgggccttc atcgtgtcgg gaatctggtt
      721 ctcgaccagg ctcgtccaga cgtagccggg cgagatgcag ttgacggtga ccccgtcgcg
      781 cgccgcttcc agggcgaccg tcttggtcag gccggcgatg ccgtgcttgg cggcgacata
      841 ggccgacttg ttggggcttg cgaccaggct gtgggccgac gccgtgttga tgatccggcc
      901 ccatttcctc gcccgcatcg ccggcagcgc cagccgggtc gtgtggaacg ccgccgacag
      961 gttgatggcg atgatcgcgt cccatttccc gggcgggacg ctttcgaccg gcgacacgtg
     1021 ctggatgccg gcattgttga ccagcacatc gggcgcgccg agctcggccg agcagcggtc
     1081 gagcatcgcc tcgatcgccg acgggtcgga caggtcggcg ccgtcgtgga ccgctccaag
     1141 ctcgccgcac aaccgttcga tctcttgccg gtcgccgaac ccgttgagca tcaccgccgc
     1201 gccttcggcc gccagcgcgc gcgcgatctc cagcccgatg cccgacgtcg acccggtgac
     1261 cagcgcgacc ttgccttcga gaatcatgcg ccctccttcg tcagcgctcc ttgtccgggc
     1321 ggaggcgatt tgcaaccgcc acggccgctc gcgcattgga gaggctcaac gggaggacgg
     1381 cggatgcgcc taggcgacga agatcccagc ggtcatttca tcgatcggac cggccgcggc
     1441 gggttcggcg gcggcggcag caacctgctc ggctgcctgc tgccgctggt ggcgagcaag
     1501 ttcgggatcg tcggagtgct cgtcctgctg ctcggctatt gcgcgctgac cagcctcggc
     1561 ggcggcggac tgttgcccgg cggcgggacc agcaccacgg ctccgtccgg ccagtcgacc
     1621 atcgatccca acacccgcga tctgctggcc cgggtgctcg gctcgaccga gcgccgctgg
     1681 agcgagattt tcgcccgcac cggcgctcag taccagccga ccaaaatgat cgcttactcg
     1741 aacaccgacc agtcgggctg cggcgcggcg caggcggcaa tgggcccgtt ctactgcccg
     1801 accgaccaga gcatctacat cgacccgcaa ttcttcagcg aattggcgca gcgcttcggc
     1861 gcgcccggcg atttcgcgat ggcctatgtg atcgcccacg aggtcggcca ccacgtccag
     1921 aacctgcagg gcacgctcga gagcgcgcag cgggcgcagg catcggccag ccgcaccgag
     1981 ggcaaccaga tccaggtcgc ggtcgagctt caggcggact gctacgcggg cgtgtgggcg
     2041 gcgcgcgacc agaatctgct ggagcccggg gatgtcgagg aggggatgcg cgcggccgag
     2101 gcgatcggcg acgacacgct gcaaaagcag atgcaggggc gggtggtccc cgaaagcttc
     2161 acccacggca ccgcggccca gcgccaggcg gcgctccgcc gcgggctgga gaccggcgac
     2221 ccggccgcct gccgcttcta gagccggacc agcatcttgc cggtgttggc gccctcgaac
     2281 aggccgagga aggcgtcggg catccgctcg agaccctcga cgatggtttc gcgtgacttg
     2341 acgtggccgc tcgcgatcca gccgcccatg tcgcggtaga attcggccag gcgcggcaga
     2401 tagtcggtga agatgaagcc ctgcagccgg atgcgcatgg cgatgatccg catgatgtag
     2461 cgcaggcaca tcggctcggc cgcattatag ccgccgatca tgccgcagat cgcgaagcgg
     2521 gcgttgcggc gcgcgagcgc caaagcggcg tcgagatggt cgccgccgac attgtcgaaa
     2581 tagacgtcga ttccgtcttg agcggcggcg gcgagcgact tgacgaccgg cccggcggtg
     2641 tagtcgacga ccgcgtcggc gcccagcgag cggacgaact cgcacttggc cgcgccgcgg
     2701 gccgagccga tcacggtcat gcccctggcc ttggcgatct ggaccaccgt cgagccgacc
     2761 gccccggcgg cggccgagac gaagaccaaa tcgccttctt tagcctgggc cgcgtcgagc
     2821 agcccgaaat aggccgtgcc gccggtgagg ccgaggttgc cgaggaacgc ctgcggctcg
     2881 accccgaggt cggggagccg ggtgaccgcc gcggccggaa gcaccgcctc gtcgcgccag
     2941 ccggccatgt ggacgacgag gtcgcccggc ttgaagccct cggccctgga ttcgaccacc
     3001 tcgccgagag ccccgccctc catcggcgcg ccgagcgcga acggcggcac gtagcttttg
     3061 acgtcgttca tccggccgcg catgtagggg tcgaccgaca gccagcggtt ggcgacgcgc
     3121 accatgccct cgccgaggcc gggcagctcg atcggcttca attcgaaatt gtcggcggtc
     3181 ggcagccctt ccgggcggct cttcaggtgc caggcgcggg ccattgccat ctcctctcgg
     3241 gc
//
LOCUS       DASTZO010000072         6048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1154638,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000072 DASTZO010000000
VERSION     DASTZO010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154638"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..364
                     /locus_tag="VFU20_01895"
     CDS             <1..364
                     /locus_tag="VFU20_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5285246.1"
                     /translation="GDVVGDYVRATVKGAGPVPRIPPLRLLGGLEAQSDRWDGRVEVE
                     WVDDQERVAAFENPTDGHTLVNASVAWRPWGKERETAVVLSANNIFDVDARRHASFTK
                     DYVPMAGRDIRLSARFSF"
     gene            424..1314
                     /locus_tag="VFU20_01900"
     CDS             424..1314
                     /locus_tag="VFU20_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU5285247.1"
                     /translation="MSAGHSHGHVHASDYGRAFAVGIALNLGFVVVETVYGFIAGSMA
                     LIADAGHNLSDVLGLMVAWAGAKMARTSRSPRFTYGLKKASILAALVNALLLLVAIGA
                     IAAEAIRRLFHPAVADGATVMAVAAVGIVINGATALLFAGGRAHDINIRGAFLHMSAD
                     AAVSAAVVVAGFLILSTGQQWIDPVMSLAIAGVIFWSSISLLKESVWMSLAGVPAGIE
                     LDEVEAELGALDGVLAVHDLHVWPLSTTETALTAHLVAPAGEPDRLLGAARELLRARF
                     QIAHSTIQVERQAPGDCGEC"
     gene            1320..2522
                     /locus_tag="VFU20_01905"
     CDS             1320..2522
                     /locus_tag="VFU20_01905"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEU5285248.1"
                     /translation="MPVTHLECSLTGERYAAGQVHGLSSAGKPLLVRYDVEEARATLT
                     RDDLALRERTMWKWRELLPLPDGVAPVSLGEPETPIVPLARTAERAGASSLLVKDEGR
                     LPTGSFKARGLAMAVTMARHFGVHRMAIPTNGNAGAALAAYGARAGIETVVICPAETP
                     EINVRETAAYGAKVYVADGQIDECGRLVAEGAAEGRWFDCSTLKEPYRLEGKKVMGFE
                     LAEQLGWTLPQAIFYPTGGGTGLIGMWKAFDELGSVGLIGPPRPRMFAVQAAGCAPIV
                     RAFERGDEFAERWEDPATVATGIRVPRAVGDFLILRAVRDSGGAALAVEEDAILEAVD
                     DVARDDGLLLCPEGGAVLAGWRLALERGLVGRGETVLLFNCASGNKYPLPDRARRMVL
                     ADARPRDL"
     gene            2676..3491
                     /gene="xth"
                     /locus_tag="VFU20_01910"
     CDS             2676..3491
                     /gene="xth"
                     /locus_tag="VFU20_01910"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU5285249.1"
                     /translation="MPKNLVDKGAATRRLRIASYNINGITTRLEVLLRWLGEFQPDIA
                     ALQELKTLDERFPQKELEQAGYASIWHGQKSWNGVAILSRVGDPVETRRGLPGDPEDR
                     HSRYIEAAVCGLLVGNLYLPNGNPWPGPKFDYKLAWMDRLHVHARELLDSGIPAMLIG
                     DFNVIPTDRDVYKPERWAKDALFSPQARTRFERLLGQGWTDALRALHPDERIYTFWHY
                     WRNSFQHDAGIRIDHALLSPSLAPKLKAAGVDRTPRGWEKTSDHAPLWVELEV"
     gene            complement(3583..3658)
                     /locus_tag="VFU20_01915"
     tRNA            complement(3583..3658)
                     /locus_tag="VFU20_01915"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3623..3625),aa:Thr,seq:tgt)
     gene            complement(3675..4526)
                     /locus_tag="VFU20_01920"
     CDS             complement(3675..4526)
                     /locus_tag="VFU20_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285250.1"
                     /translation="MRSRFAAFVAIDWSGAKGRRHKGIAIAEARGDSAPRLVRPGHAW
                     SREEVLEWLLKRAAAEPTLFGFDFSFAPPLVERGEYLPGEIGVPATAREFWAYVDRLC
                     GDEDLGAASFLEAAHRRHFYFGIADGAKAPFMRFRQCDQALNAQGGRKTASAYDAIGA
                     AQVAKASFSGMRLLHRLDGKVAIWPMDPLPEHGSAVVEIYTRIYLRRAGLSGRKLRTR
                     AELNRALTGLGSAPTRLRFEPNDHQTDALVTAAGMRALAADPNAFAPAGLTPHIARTE
                     GWTFGVI"
     gene            complement(4523..5839)
                     /locus_tag="VFU20_01925"
     CDS             complement(4523..5839)
                     /locus_tag="VFU20_01925"
                     /inference="COORDINATES: protein motif:HMM:NF033912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HEU5285251.1"
                     /translation="MTMYRSDAPTAYWRDQMIEWGPRVIFAILILVATHFIAKMVQWA
                     VAKGIDRVPVLKRHPQAGGQSVGVELGRLAYWLVWLVGLIAALQPLGLSGVLTPVTSL
                     TNEVFAFLPRLLGAGLFFFAGLILARIVRHVIEAALGALNIERLLGRAGISIGEPPLA
                     VDETGVATEGAAPARSSIARAVGVTVSATIIIFAAIAALQILQISAISDPATAMLNSI
                     ALAIPRVLAALVWLAIAFLIGRWVKTLVETVLPSLGFDDSVRALGIMPASSNPSRVVG
                     AIAMTAILLAAAIEAARQLGGDSVAVLLFQITELGGKVIFGTVIIVAGLFLARILSSL
                     VGSSTGEAGYAQTIVKYAIIALFTAIGLTFMGLADQIVMMAFGLILGSAAVATALAFG
                     LGGRDAAARILEEWAEERAPPARLPPPRIRKAPPPEPDDDAQPPLV"
     gene            complement(5846..>6048)
                     /locus_tag="VFU20_01930"
     CDS             complement(5846..>6048)
                     /locus_tag="VFU20_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEU5285252.1"
                     /translation="IGYGQGYTYDHDADEGFSGDNYWPEEMEPQTYYQPTDRGFEAKV
                     AERIAYWDRMRAKRAKGNDDSE"
BASE COUNT          911 a         2082 c         2150 g          905 t
ORIGIN      
        1 cggcgacgtc gtcggcgatt atgtccgggc gacggtcaag ggagcgggcc cggttccgcg
       61 gattccgccg cttcgcctgc tcggcggact cgaggcgcaa tcggaccgct gggatgggcg
      121 ggtcgaggtc gagtgggtcg acgaccagga gcgcgtcgcg gcgttcgaga acccgaccga
      181 cggccacaca ttggtcaatg cctcggttgc gtggcggccg tggggcaagg agcgggagac
      241 ggccgtggtg ctctcggcga acaatatttt cgacgtcgat gcgcggcgcc atgcctcgtt
      301 caccaaggac tatgtgccga tggccggccg cgacatccgg ttgagcgccc gcttcagctt
      361 ctaggagccg atcggcgggg cgccgtttgt cttcgcgcgc ggctatggct aggccccgcc
      421 gcgatgagcg ccggacattc ccatggtcac gtccacgcgt ccgactacgg ccgcgcgttc
      481 gcggtcggga tcgcgctcaa cctcggcttc gtcgtcgtcg agaccgtcta cgggttcatc
      541 gccggctcga tggcgctgat cgccgacgcc ggccacaatc tgtccgacgt gctcgggctg
      601 atggtcgcct gggccggggc gaagatggcg cggacgtcgc gctcgccgcg cttcacctac
      661 gggctgaaga aagcgtcgat cctcgcggcg ctggtcaacg ccctgctgct gctggtcgcg
      721 atcggcgcca tcgccgccga agcgatccgg cggctgttcc atccggcggt cgcggacggc
      781 gcgacggtga tggcggtcgc ggcggtcggg atcgtcatca acggcgccac cgctttgctg
      841 ttcgcgggcg gccgggcgca cgacatcaac atccgcggcg ccttcctgca catgagcgcc
      901 gacgccgcgg tgtccgccgc cgtggtcgtc gccggcttcc tgatcctgtc gaccggccag
      961 cagtggatcg acccggtgat gagcctggcc atcgccgggg tgatcttctg gagcagcatc
     1021 tcgctcctca aggaatcggt gtggatgtcg ctcgccggag tgcccgccgg gatcgagctc
     1081 gacgaggtcg aggccgagct tggcgcgctc gacggggtgc tggcggtgca cgacctgcac
     1141 gtctggccgc tcagcacgac cgagaccgcc ctgaccgcgc atctggtcgc gccggcgggc
     1201 gagcccgacc ggctgctggg cgccgcgcgc gagcttctgc gcgcccggtt ccagatcgcc
     1261 cattcgacca tccaggttga gcggcaggcg cccggcgact gcggcgagtg ctagcggcga
     1321 tgccggtcac ccacctcgaa tgctcgctga ccggcgaacg ctatgcggcc ggacaggtgc
     1381 acggcctgtc gagcgccgga aagccgctgc tggtgcgcta cgatgtggag gaagcgcgcg
     1441 cgaccctgac ccgcgatgat ctcgccttgc gcgagcggac gatgtggaaa tggcgcgagc
     1501 tgctgccgct tccggacggc gtcgcgccgg tcagcctcgg cgagcccgag accccgatcg
     1561 tgccgctcgc ccgcaccgcg gagcgcgccg gcgcctcgag cctgctggtc aaggacgagg
     1621 gccggctgcc gaccggctcg ttcaaggcgc gcgggctggc gatggcggtg accatggcca
     1681 ggcatttcgg ggttcaccgg atggcgatcc cgaccaacgg caatgccggc gccgcgctcg
     1741 ccgcctacgg cgcccgggcg gggatcgaga ccgtcgtgat ctgcccggcc gagacgcccg
     1801 agatcaacgt gcgcgagacc gcggcctatg gcgcgaaggt ctatgtcgcc gacggccaga
     1861 tcgacgagtg cggacggctg gtcgccgagg gggccgcgga agggcgctgg ttcgattgct
     1921 cgacgctcaa ggagccgtac cggctcgagg gcaagaaggt gatgggcttc gagctcgccg
     1981 agcagcttgg ctggacgctg ccgcaggcga tcttctaccc gaccggcggc ggcaccggcc
     2041 tcatcggcat gtggaaggcg ttcgacgaac tggggtcggt cgggctgatc gggccgccgc
     2101 gcccgcggat gttcgcggtc caggcggccg gctgcgcacc gatcgtccgg gcgttcgaac
     2161 gcggcgacga gttcgccgag cgctgggagg acccggccac ggtcgcgacc ggaatccggg
     2221 tgcccagggc cgtcggcgat ttcctgatcc tgcgcgcggt gcgcgacagc ggcggcgcgg
     2281 ccctggcggt cgaggaggat gcgatcctcg aggcggtcga cgacgtcgct cgcgacgacg
     2341 gcctgctgct gtgcccggag ggcggagcgg tgctcgccgg ctggcggctg gcgctcgagc
     2401 gagggctggt cgggcgcggc gagaccgtcc tgctgttcaa ttgtgccagc gggaacaaat
     2461 acccgctgcc ggatcgggcg cggcggatgg tgctggccga tgctcggccg cgcgatctct
     2521 agggccccaa gagatctcta ggggcccaaa cacgtaagcc ccggcaaccg aagttgccgg
     2581 ggctcccatg cttctcttcc cgaaggattc gaagcacgca aacagaacga tttgcgcctc
     2641 gggtggttcc tgccgcgatg gccaaaaaac ccgttttgcc caagaatctt gtggataaag
     2701 gcgcggcgac tcgccggctt cggatcgcca gctacaacat caacggaatc accacccgcc
     2761 tcgaggtgct gctgcgctgg ctcggcgagt tccagcccga catcgccgcc ctgcaggagc
     2821 tcaagaccct cgacgagcgg tttccgcaaa aggagctcga gcaagccggc tatgcgagca
     2881 tctggcacgg ccagaaaagc tggaacgggg tcgcgatcct gagccgcgtc ggcgacccgg
     2941 tcgagacccg gcgcgggctg cccggcgacc cggaggaccg ccacagccgc tacatcgagg
     3001 cggcggtctg tggccttctg gtcggcaacc tctatctgcc caacggcaac ccgtggccgg
     3061 ggcccaagtt cgactacaag ctcgcctgga tggaccggct gcacgtccac gcccgggagc
     3121 tgctcgacag cggcatcccg gcgatgctga tcggcgactt caacgtcatc ccgaccgacc
     3181 gggacgtcta caagcccgag cgatgggcga aggacgcgtt gttctcgccg caggcgcgga
     3241 ccaggttcga gcggctgctc gggcagggct ggaccgacgc cctccgcgcg ctccacccgg
     3301 acgagcggat ctacaccttc tggcactatt ggcggaattc gttccagcac gacgccggca
     3361 tccgcatcga ccatgctctc ttgagcccga gcctggcgcc caagctgaaa gccgccggcg
     3421 tcgaccgcac cccgcgcggc tgggaaaaga ccagcgacca cgcgccgctg tgggtcgagc
     3481 tggaggtgta ggccgagcga tcgccatgct cgcttgtccc ccggatggcc ctgcgcgcgg
     3541 atcgctgtgc tcgcttgtcc cctggacaag ctgcgccagc gatggtgccg gatgcaggat
     3601 tcgaacccgc gaccttcggt ttacaaaacc gctgctctac caactgagct aatccggccc
     3661 cgcttcggtt tgcgtcagat gacgccgaac gtccaccctt cggtgcgggc gatatggggc
     3721 gtcagcccgg ccggggcaaa ggcgttcgga tcggcggcga gcgcgcgcat cccggcggcg
     3781 gtgaccagcg cgtcggtctg gtggtcgttg ggctcgaagc gcagccgtgt tggcgcgctt
     3841 cctaggccgg tcagcgcccg gttgagctcg gcgcgggtgc gcagcttgcg gcccgagagg
     3901 ccagcgcgcc gcagatagat gcgggtgtag atctcgacca ccgcgctgcc gtgctcgggc
     3961 agtgggtcca tcggccagat cgcgaccttg ccgtccagcc ggtggagcag ccgcatgccc
     4021 gagaagctcg ccttcgcgac ctgcgcggcg ccgatcgcat cataggcgct ggcggtcttg
     4081 cggccgccct gcgcgttcag cgcctggtcg cactggcgga atcgcatgaa cggcgccttc
     4141 gccccgtcgg cgatgccgaa atagaaatgc ctgcggtgcg cggcctcgag gaagctcgcg
     4201 gcgccgaggt cctcgtcgcc gcaaagccgg tcaacatagg cccagaactc gcgcgcggtt
     4261 gcgggcacgc cgatctcgcc gggcagatac tcaccgcgct cgaccaaggg cggcgcgaag
     4321 ctgaagtcga agccgaacag agtcggctcc gccgcggcgc gcttcaggag ccactcgagc
     4381 acctcctcgc gcgaccaggc gtggcccggg cggacgaggc gcggcgcgga atcgccgcgc
     4441 gcctcggcga tggcgatgcc cttgtggcgc cggcccttgg cgcccgacca gtcgatcgcg
     4501 acgaaggcgg cgaagcggct cctcacacca agggcggctg ggcgtcgtcg tcgggctcgg
     4561 gcggcggcgc cttgcggatt cgcggcggcg gcagccgcgc cggcggcgcc cgctcttccg
     4621 cccactcctc gaggatccgc gccgccgcgt cgcgcccgcc gagcccgaag gccagcgcgg
     4681 tcgccaccgc ggccgagccg aggatcagcc cgaacgccat catcacgatc tggtcggcga
     4741 gccccatgaa ggtcagcccg atcgcggtga acagagcgat gatcgcatat ttgacgatcg
     4801 tctgcgcgta gcccgcctcg cccgtcgagc ttccgaccag cgacgacagg atgcgcgcga
     4861 ggaacaggcc ggcgacgatg atcaccgtgc cgaagatcac cttgccgccc agctcggtga
     4921 tctggaacag cagcacggcg acgctgtcgc cgcccagctg ccgcgccgcc tcgatcgccg
     4981 cggcgagcag gatcgcggtc atcgcgatcg ccccgacgac ccgcgacggg ttggagctcg
     5041 ccggcatgat cccgagcgcg cgcacgctgt cgtcgaagcc gaggctcgga agcacggtct
     5101 cgaccagggt cttgacccag cggccgatca ggaaggcgat cgccagccac accagagcgg
     5161 cgagcacgcg cgggatcgcc agagcgatcg agttgagcat cgccgtcgcg gggtcggaga
     5221 tcgccgagat ctgcaggatc tgaagcgcgg cgatggcggc gaagatgatg atcgtcgccg
     5281 agacggtgac cccgaccgcc cgggcgatcg agctccgcgc cggcgccgct ccttcggtgg
     5341 cgaccccggt ctcgtcgacc gccagcggcg gctcgccgat gctgatcccg gcgcggccga
     5401 gcagccgctc gatgttgagc gcgccgagcg ccgcctcgat cacgtggcgg acgattcggg
     5461 cgaggatcag cccggcgaag aagaacagcc cggcgccgag cagcctcggc aggaaggcga
     5521 acacttcatt ggtcaggctg gtgacggggg tgaggactcc cgacaggccg agcggctgga
     5581 gagcggcgat cagcccgacc agccacacta gccagtaagc gagccggccg agctcgaccc
     5641 cgaccgactg gccgccggcc tgcgggtggc gcttgagcac cggcacccgg tcgatcccct
     5701 tggccaccgc ccattgcacc atcttggcga tgaagtgcgt ggcgaccagg atcaggatcg
     5761 cgaagatcac ccgcgggccc cattcgatca tctggtcgcg ccagtaggcg gtcggcgcat
     5821 cgctccgata catggtcatc ctccgctatt cgctgtcgtc gtttccctta gcgcgtttgg
     5881 cgcgcatccg atcccaataa gcgatccgtt cggcgacctt ggcctcgaag ccgcggtcgg
     5941 tcggctggta ataggtctgc ggctccatct cctcgggcca gtagttgtcg ccggaaaagc
     6001 cctcgtcggc gtcgtggtcg tacgtgtagc cctggccgta gccgatct
//
LOCUS       DASTZO010000073         2796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_710473,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000073 DASTZO010000000
VERSION     DASTZO010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_710473"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VFU20_01935"
     CDS             <1..1124
                     /locus_tag="VFU20_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5285253.1"
                     /translation="GGLPERIAESGAQTLLELRRLRLQELIRSHRGQLESDGSGQPVA
                     RGRLVAIDPDPASLQLAARAGFFILTEERHGELGLRVVHLRVPRGMSARDALKRLRSA
                     APRIEADFDHLFEPAGGELLPVAGALAAAATSGPGRKIGMIDGGVASHPSLARAPIEQ
                     NGFSGSPQPTGHGTAVASLIVGDQGPFRGAARGSSLLVADVYGGSRAAGSATAVVRAL
                     AWLARHRPSVINISLVGPSNRLVARAVGAVRARGIQLVAAVGNDGPAAPPQYPASYPG
                     VVAVTGVDARGRALPEAGRATHVDFAAPGADMAAALPGEGYAKVRGTSFAAPLAAARL
                     ALVGSPQRLVAEARPGKGRVGRGIVCADCRVAPKAVRAK"
     gene            1215..2186
                     /locus_tag="VFU20_01940"
     CDS             1215..2186
                     /locus_tag="VFU20_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285254.1"
                     /translation="MKRFILIAGATITISATPAYAGGSGGLLGGVTGTVNQVVGGSGG
                     LLGGSLGNPVGSLGNCLCETVNGVLRNPGSLANSIVGLGSGSGSGNGNNRSGKSVGLA
                     GTVTGAANAAASVTGTGHGGQRGLNLAGTVNGVANGAASVTRIGHGRGLNVSGAVSSA
                     ANATASLTKAGHSAILNGTASGDGSANARASRKGARANANANGSANGSALISLNRGAG
                     SGKLLNLGGAPGGVTLPSLGGTGGNPVGNLADCLCETAGQLLGKPASGGSSGSKSGNN
                     GAANNTSANSSGAKNSSANGSRQASTGSKRASQSGGGPWPVERHQSH"
     gene            2372..>2796
                     /locus_tag="VFU20_01945"
     CDS             2372..>2796
                     /locus_tag="VFU20_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285255.1"
                     /translation="MTKSILIAGAAIALAAAPANAGGLLGGVTGAVNGTLGGAVGGTV
                     NGSLGGLGGAVNGSLGGVGRAGGQGGLIGNSGSLTGSVSGLAGGGLDGSANGDGSANA
                     VGLAGVVTGAANAAGSATGAANGAGSNAFGLAGTVNGVA"
BASE COUNT          398 a         1039 c         1014 g          345 t
ORIGIN      
        1 tcggcgggct gccggaacgg atcgcggaat cgggcgccca gacgcttctc gagctccgcc
       61 ggctgcggct ccaggaactg atccggtcgc accgcggcca gctcgaaagc gacggcagcg
      121 gccagccggt ggcgcgcggc cggctggtcg cgatcgaccc cgatccggct tcgctgcagc
      181 tcgcggcgcg ggccggcttc ttcatcctca ccgaagagcg ccacggcgag ctcggcctcc
      241 gcgtcgtcca cctgcgggtg ccgcgcggca tgagcgcgcg cgacgcgctg aagcggctgc
      301 ggtcggcggc gccgcggatc gaggcggact tcgaccatct gttcgagccg gcgggaggcg
      361 agctgcttcc ggtcgccggg gccctcgccg ccgccgcgac cagcggcccg gggcgcaaga
      421 tcgggatgat cgacggcggc gtcgcctcgc acccgtcgct ggcccgcgct ccgatcgagc
      481 agaacggctt ttccggaagc ccccagccga ccggccacgg caccgcggtc gcgtcgctga
      541 tcgtcggcga ccagggcccg ttccggggcg ccgcccgcgg ctcgtccctg ctggtcgccg
      601 acgtctatgg cggcagccgc gccgccggct cggcgaccgc ggtcgtccgg gcgctcgcct
      661 ggctggcccg ccaccggccg agcgtgatca acatcagcct cgtcggcccg tcgaaccggc
      721 tggtcgcacg ggcggtcgga gcggtccggg cgcgcggcat ccaactcgtc gccgcggtcg
      781 gcaacgacgg gcccgcggcg ccgccgcaat atccggcttc ctatccgggc gtggtcgcgg
      841 tcaccggggt cgatgcgcgc ggccgggcgc tgcccgaggc aggcagagcg acgcatgtcg
      901 atttcgccgc tccgggcgcc gatatggccg ccgcccttcc gggcgagggc tatgcaaagg
      961 tccgcggcac cagcttcgcg gcgccgctcg ccgccgcccg cctggcgctg gtcgggtcgc
     1021 cgcagcgcct cgtcgccgaa gcccgccccg gcaagggccg ggtcggccgc gggatcgtct
     1081 gcgccgattg ccgggtcgcc cccaaggccg tccgcgccaa atagattacc tccaaaatgg
     1141 atgaaccggc ccgcgggcat cgttctcgtc gtgaagggag ccttccaggc ccctttcggt
     1201 aaaggagaag aacgatgaaa aggttcatcc tgattgccgg ggcaacaatc accatctcag
     1261 caaccccggc ttatgccggc ggatcgggcg gactgctcgg cggtgtcacc ggcaccgtga
     1321 accaggtcgt cggcggctcc ggcgggctgc tcggcgggtc gctgggcaac ccggtcggct
     1381 cgctcggcaa ctgcctgtgc gagaccgtca acggtgtgct tcgcaatccg ggctcgctgg
     1441 ccaacagcat cgtcgggctc ggatccggca gcggctcggg caacggcaac aaccggtccg
     1501 gcaagagcgt cgggctggcc ggcaccgtca ccggcgccgc caatgcggcc gcttcggtga
     1561 ccgggaccgg ccatggcggc cagcgcggcc tcaacctggc cggaaccgtc aacggcgtcg
     1621 ccaacggcgc ggcttcggtg acccggatcg gccacggccg cggcctcaac gtcagcggcg
     1681 cggtcagcag cgccgccaac gccacggcct cgctgaccaa ggcgggccat tcggcgatcc
     1741 tcaacggcac ggcgtcgggc gacggctcgg ccaacgccag ggccagccgc aagggcgcca
     1801 gggccaatgc caacgccaac ggctcggcca acgggtcggc gctgatcagc ctcaaccgcg
     1861 gcgccggctc cggcaagctg ctcaacctcg gcggcgctcc gggcggagtc acgctgccca
     1921 gcctcggcgg aaccggcggc aaccccgtcg gcaatctcgc cgattgcctg tgcgagaccg
     1981 cgggccagct gctcggcaag cccgcgagcg gcggcagctc cggcagcaag agcggcaata
     2041 acggcgctgc caacaacacc tccgccaaca gcagcggcgc caagaacagc tccgccaatg
     2101 gctcgaggca ggccagcacc ggctcgaaga gggcgtcgca aagtggcggt ggcccgtggc
     2161 cggtggagcg gcaccagtcg cactaacgat caccttctac cgaaggctca gcggcccgcc
     2221 gccctcgctc ccggggcggc gggctttttt gccacagtcc gccggaagtt tcgtccccgc
     2281 cgggatgaag ctccgccggg ccaccgttcc cattggaccg gggcgggagg aaacctctcc
     2341 ccgtacagtg acgacacaaa gggagaatga gatgacgaaa tccatcctga tagccggagc
     2401 ggccatcgct cttgccgccg ccccggcgaa cgccggcggg ctgctcggcg gagtgaccgg
     2461 cgccgtcaac ggcacactcg gcggagcggt cggcggaacc gtcaacggct cgctcggcgg
     2521 gctcggcggg gccgtcaacg gctcgctcgg cggggtcggc cgggccggcg gccagggcgg
     2581 cctgatcgga aattccgggt cgctgaccgg cagcgtcagc gggctcgccg gcggcggcct
     2641 cgacgggtcg gcgaacggcg acggctcggc gaacgcggtc ggcctcgccg gcgtcgtcac
     2701 cggcgcggcc aacgcggccg gctcggcgac cggagcggcg aacggcgccg gatcgaacgc
     2761 attcggcctg gccggcaccg tgaatggcgt cgccaa
//
LOCUS       DASTZO010000074         2569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_423774,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000074 DASTZO010000000
VERSION     DASTZO010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_423774"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VFU20_01950"
     CDS             <1..924
                     /locus_tag="VFU20_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5285256.1"
                     /translation="PILIVGEPGSGKETFARAIHAASLRARGALVTVDCKAIPANIID
                     SQLFGHEKGAFPGAFAAKTGKIVEADTGTLLLDEIAALPAETQQMLDRVLATGEVRPV
                     GCNGSNSVDVRVIATTSRPLPPEFDAGLAERIGATVVTLPPLRDRSGDIPALARHLLA
                     RFADQARLRPLSIGNDALAVLMRYGWPGNVRQLAGVLFRAGLHCQGGALTAEDFPHIA
                     IQSRFSGRRNDFAPEITQSKSEEALVGAPSVTLYRNDGHLRPLEEIEADIIRLAIGHY
                     RGRMTEVARRLGIGRSTLYRKLGELGIDTAA"
     gene            958..1737
                     /locus_tag="VFU20_01955"
     CDS             958..1737
                     /locus_tag="VFU20_01955"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HEU5285257.1"
                     /translation="MPLLYLLAACGGAESKAPPAPIAARAPCNQHLFEGARFTVCDPD
                     NGRIELVAAARDETPVRRLADLEKSLGRRASTVAFAMTGGMYGEDGRPIGLAIVEGRQ
                     VRAINRRKGGGNFHLMPNGVFQVRRGGRAEIVTTANWKPSPDIRVATQSGPMLVIAGR
                     LHPAFDHDGSSRHIRNGVGIAPDGQPLFVISEDAVSLGKFARFFRDRLKCRDALFFDG
                     AVSALWDPANHRRDVTVPLGPLLVAFKPAESKPGRAGPARP"
     gene            complement(1688..>2569)
                     /gene="folP"
                     /locus_tag="VFU20_01960"
     CDS             complement(1688..>2569)
                     /gene="folP"
                     /locus_tag="VFU20_01960"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU5285258.1"
                     /translation="RAQWQALTARRAPLQLGERTVRLDQPQVMGVLNVTPDSFSDGGR
                     FADAEAAAAAGADMAAQGAAIVDVGGESTRPGAKPVWEGDESERVAPVIRQLAASGAA
                     VSVDTRKSEVMTAALAAGARLVNDVSALTYDPRSAEVVAAAGVPVVLMHHQGSPETMQ
                     DAPRYDDVLVEVYLWLEERIAAAEGAGIARSNILVDPGFGFGKTVAHNLELMNGLALL
                     HSLGCPVVLGASRKRTIGALSNEAPADQRLGGSIAFALKAAGQGAQVIRAHDVFETVQ
                     ALRVWRGLRDQALTPRA"
BASE COUNT          369 a         1001 c          846 g          353 t
ORIGIN      
        1 ccgatcctga tcgtcggcga gcccggcagc ggcaaggaaa ccttcgcccg ggccatccac
       61 gcggccagcc tgcgcgcccg cggcgcgctg gtgaccgtcg actgcaaggc gatcccggcc
      121 aacatcatcg acagccagct gttcggccac gaaaagggcg ccttccccgg cgcgttcgcc
      181 gccaagaccg gcaagatcgt cgaggccgac acgggcactc tgctgctcga cgagatcgcc
      241 gcgcttccgg ccgagaccca gcaaatgctc gaccgggtgc tcgccaccgg cgaagtccgt
      301 ccggtcggct gcaacggcag caattcggtc gacgtccggg tgatcgcgac caccagccgc
      361 ccgctgccgc ccgaattcga cgccggcctc gccgagcgga tcggcgcgac cgtggtcacc
      421 ctgccgccgc tccgcgaccg cagcggcgac atcccggcgc tcgcccgcca tctgctcgcc
      481 cgcttcgccg accaggcgcg gctgcgcccg ctgtcgatcg gcaacgacgc gctcgcggtg
      541 ctgatgcgct acggctggcc gggcaacgtc cgccagctcg ccggggtgct gttccgcgcc
      601 ggcctccact gccagggcgg cgcgctcacc gccgaggatt tcccgcacat cgcgatccag
      661 tcgcgcttca gcggccgccg caacgacttc gcgcccgaga tcacccagtc gaagagcgag
      721 gaggccctcg tcggcgcgcc gtcggtcacg ctctaccgca acgacggcca tttgcggccg
      781 ctggaggaga tcgaggccga catcatccga ctcgccatcg gccattaccg cggccgcatg
      841 accgaggtcg cccggcgcct cgggatcggc cgctcgacgc tctaccgcaa actgggcgag
      901 ctcggaatcg acaccgccgc ctgaggcgct agcttcgcgg ccatgcgaag cgcgctgttg
      961 ccactgcttt acctgctcgc cgcgtgcggc ggggccgagt cgaaagcgcc gccggccccg
     1021 atcgccgcgc gggcgccgtg caaccagcac ctgttcgaag gcgcccgctt cacggtgtgc
     1081 gatcccgaca atggacggat cgagctggtc gccgccgccc gcgacgaaac cccggtccgc
     1141 cgcctggccg acctggagaa gagcctcggc cgccgcgcca gcaccgtcgc cttcgcgatg
     1201 accgggggca tgtacggcga ggacggccgc ccgatcggcc tcgcgatcgt cgagggccgg
     1261 caggtgcgcg cgatcaaccg gcgcaagggc ggcggcaatt tccacttgat gcccaacggc
     1321 gtcttccagg tgcgccgcgg cggccgggcc gagatcgtca ccacggcgaa ctggaaaccg
     1381 tctcccgaca tccgcgtcgc gacccagtcg gggccgatgc tggtgatcgc cggcaggctc
     1441 cacccggcgt tcgaccatga cggcagctcg cgccatatcc gcaacggggt cggaatcgcc
     1501 cccgacggcc agccgctctt cgtcatcagc gaggatgcgg tgtcgctcgg caagttcgcg
     1561 cgcttcttcc gcgaccggct gaagtgccgc gacgctctgt tcttcgacgg cgcggtcagc
     1621 gctctgtggg acccggccaa ccaccgccgc gacgtcaccg tgccgctcgg cccgttgctc
     1681 gtcgcgttca agcccgcgga gtcaaagcct ggtcgcgcag gccccgccag accctgagcg
     1741 cctggacggt ctcgaacacg tcgtgggcgc ggatgacctg cgccccctgc ccggccgcct
     1801 tgagcgcgaa cgcaatgctt ccgccgaggc gctggtccgc cggcgcctcg ttcgacagcg
     1861 cgccgatggt ccgcttgcgg ctcgccccga gcacgaccgg gcaaccgagc gaatgcagca
     1921 gcgccagccc gttcatcagc tcgagattgt gggcgacggt cttgccgaag ccgaagccgg
     1981 gatcgacgag gatgttcgaa cgggcgatcc cagcaccttc cgccgccgcg atccgctcct
     2041 cgagccagag atagacctcg accaacacgt cgtcgtagcg cggcgcatcc tgcatcgtct
     2101 ccgggctgcc ctggtggtgc atcagcacga ccgggacccc ggccgcggcg accacctcgg
     2161 ccgagcgcgg gtcgtaggtc agcgccgaga cgtcgttgac cagccgagca cccgccgcga
     2221 gcgccgcggt catcacctcg gacttgcggg tgtcgaccga caccgccgct ccgcttgccg
     2281 cgagctggcg gatcaccggc gccacccgct cggactcgtc gccttcccac accggcttcg
     2341 cgcccggccg ggtcgattcg cccccgacgt cgacgatcgc cgcaccttgc gcggccatgt
     2401 cggcgcccgc tgcggccgcc gcctcggcat cggcgaaccg gccgccgtca gagaaactgt
     2461 cgggcgtgac gttgagcacg cccatcacct gcggctgatc gaggcgcacc gtgcgctcgc
     2521 ccaattgcag cggcgcgcgc ctggcggtca gcgcctgcca ttgcgccct
//
LOCUS       DASTZO010000075         4317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_606588,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000075 DASTZO010000000
VERSION     DASTZO010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606588"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..725)
                     /gene="rph"
                     /locus_tag="VFU20_01965"
     CDS             complement(9..725)
                     /gene="rph"
                     /locus_tag="VFU20_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEU5285259.1"
                     /translation="MRPSGRAPDQMRELRFEPGFTRYAEGSCLVSFGETRVLVTASVE
                     DKVPPFLRGKGKGWVTAEYGMLPRATHTRGNREAAKGKQSGRTQEIQRLIGRSLRAVV
                     DLPKLGERQVIVDCDVIQADGGTRTAAISGAWVALRIAVDKLLAAKTIAADPIRSQVA
                     AVSCGFHNGAAVLDLDYVEDSEAGCDGNFVLTDDGSIVEAQVSAEGECYDEEGLLRLL
                     RLARIGCSEIFDAQRKAVGR"
     gene            803..1837
                     /gene="hrcA"
                     /locus_tag="VFU20_01970"
     CDS             803..1837
                     /gene="hrcA"
                     /locus_tag="VFU20_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU5285260.1"
                     /translation="MTQPIGELTDRMRSIFGLVVEAYLERGQPVGSKALTGSVSLSPA
                     SIRGVMQELEERGLLTHPHTSAGRIPTETGLRLFVDGIMQASLPDERERAAIEAQIAR
                     DQPIEQALAAASAALSGLSSCAGVVMAPKQELHLKQLSFVALSPGRALAVLVGADGSV
                     ENRILTLDSGTDPAALNEVANFVNARLSGMTLAEAEARLRAEIGQRKEALDRAAAELI
                     ASGLAAWSEDHARRPVLIVRGQANLIDERAGEDLERVRRLLDELEDRQEIARLLEGAR
                     DAPGCRIFIGSENRMFALSGSSVIAAPYRGSQGQVVGVVGVIGPTRLNYARVVPMVDF
                     TAKALTRLMA"
     gene            1834..2394
                     /gene="grpE"
                     /locus_tag="VFU20_01975"
     CDS             1834..2394
                     /gene="grpE"
                     /locus_tag="VFU20_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU5285261.1"
                     /translation="MKPGTTDDEKLHEEAEEIRKETAEAAPELAEHDRLAELEKALEE
                     ANSKALYAAAETQNVRRRLEAEKVQAANYASSAFARDMLAIKDHLDRALAAVSDELRE
                     DKVASNFLDGIEATARELEAAFARQGITRIKSIGEKLDPHRHQAMMEIPTDNAEPGTI
                     VDEMQAGYMLKDRLLRPALVGVAKAP"
     gene            complement(2410..3735)
                     /locus_tag="VFU20_01980"
     CDS             complement(2410..3735)
                     /locus_tag="VFU20_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU5285262.1"
                     /translation="MTAEQVLTLLVLAAVVAALIWDKVRSDVVALTGAAFLLMTGVVR
                     PVEVQAAFASPAIIALASLFVIAYAMELSGLLDTAIRKVVALCRRIGTAGLWILIGLA
                     GSASAFLNNTPIVVLSAPVVRDVANSLGRSPKRYLIPLSYAAVLGGCCTLIGTSTNLL
                     VNDMAMVAGQPRFGIFEITPAGLMVAAAGGLHLMLFSTRLIGSGEDRDRAGVREAAQA
                     AENFREPGLAGGLIGSAQPFARQVALRPWPALASSLVFVAVVTLAALNVAPIAACAFA
                     GAVLLILLRVITANEAYGGLRPEVLMLIAGMVVLGIALDETGLANAATSALIGSMEGM
                     NPLLALVILYGTTLFATEILSNATVAVLFTPIAVSMAEAFAVSPRPFLVAIMLAGSAA
                     FATPFGYQTNVIVYQMGGYNYLDFVKVGIPLNLITWVVGIIAIRMYFPF"
     gene            3845..>4317
                     /locus_tag="VFU20_01985"
     CDS             3845..>4317
                     /locus_tag="VFU20_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285263.1"
                     /translation="MTELRPPPLRPAGSASLLGALAMLAACQPEAPDANLPKKVEQPV
                     PDLPAVPRPQPPIDRAGILAAVAQAASAAASGADDGAAQGALDGRQFEFRIRFGCRGP
                     SSKLRDEWLGWSHDPESGTLRVRAMPTLSAQEELARELGGDRFETVEGFWIPRPWL"
BASE COUNT          721 a         1441 c         1545 g          610 t
ORIGIN      
        1 gatcggtctc atcgtccaac cgcctttcgt tgcgcgtcga aaatctccga gcagccgatg
       61 cgggcgagcc gcagaagccg caacagccct tcctcgtcgt agcattcgcc ctcggcgctg
      121 acctgcgcct cgacgatcga gccgtcgtcc gtgaggacga aattgccgtc gcagccggcc
      181 tcgctgtcct cgacatagtc gaggtcgagc accgcagcgc cgttgtggaa gccgcagctg
      241 accgccgcga cctggcttcg gatcgggtcg gcggcgatgg tcttggcggc gagcagcttg
      301 tcgaccgcga tccgcaatgc gacccaggcg cccgagatgg ccgcggtgcg ggtgccgccg
      361 tcggcctgga tgacgtcgca gtcgacgatc acctgccgct cgccgagctt cggcaggtcg
      421 acgacggcgc gcagcgagcg cccgatcagc cgctggatct cctgggtgcg gcccgactgc
      481 ttgcccttgg ccgcctcgcg gttgccgcgc gtgtgggtgg cgcgcggaag catgccatat
      541 tcggccgtca cccagccctt gcccttgccg cgcaggaacg gcggcacctt gtcctcgacc
      601 gaggcggtga ccagcacgcg cgtttcgccg aagctcacca ggcacgagcc ctcggcgtag
      661 cgcgtgaagc cgggctcgaa gcgcagctcg cgcatctggt cgggggcgcg tccgctgggg
      721 cgcattcggg actcctcgtg ggctcgggtt gagcggcgct cactagaccg ggcggcggtg
      781 caatcctaga tgcacgaacg caatgaccca gcccatcggc gagctcaccg accgcatgcg
      841 gtcgattttc ggactggtgg tcgaggcgta tctcgagcgc ggccagccgg tcggctccaa
      901 ggcgctgacg ggcagcgtga gcctgtcgcc ggcctcgatc cgcggagtca tgcaggagct
      961 cgaggagcgc ggcctcctta cccatccgca cacgtccgcg gggcgaatcc cgaccgagac
     1021 cgggcttcgg ctgttcgtcg acgggatcat gcaggcgtcg cttcccgacg agcgcgagcg
     1081 ggcggcgatc gaggcccaga tcgcgcgtga ccagccgatc gagcaggcgc tggcggcggc
     1141 ctcggcggcg ctgtccggcc tgtcgtcctg cgcgggcgtg gtgatggccc ccaagcagga
     1201 actgcacctg aagcagctga gcttcgttgc actgtcgccg ggccgcgcgc tggcggtgct
     1261 ggtcggggct gacggaagcg tcgagaaccg gatcctgacg ctcgattcgg ggaccgaccc
     1321 ggcggcgctc aacgaggtcg cgaatttcgt caacgcgcgg ctgtcgggga tgaccctggc
     1381 ggaagcggag gctcggcttc gggccgagat cggccagcgc aaggaggcgc tcgaccgcgc
     1441 cgccgccgag ctgatcgcgt cggggctggc ggcgtggagc gaggaccatg cccgccgccc
     1501 ggtgctgatc gtccgcggcc aggcgaacct gatcgacgag cgcgccggcg aggaccttga
     1561 gcgcgtccgg cggctgctgg acgagctcga ggaccggcag gagatcgcgc ggcttctcga
     1621 gggcgcccgc gacgcgcccg gctgccggat cttcatcggc tcggagaacc ggatgttcgc
     1681 tctgtcgggc tcgtcggtga tcgccgcgcc ctatcgcggg agccaggggc aggtggtcgg
     1741 cgttgtcggc gtgatcgggc ccacccggtt gaactatgcg cgggtggtcc ccatggtgga
     1801 tttcaccgcc aaggcgctga cgagattgat ggcatgaaac ctggaacgac cgacgacgag
     1861 aagctccacg aagaggcgga ggaaatccgc aaggaaacgg ccgaggcggc gcccgagctc
     1921 gccgagcacg atcggctggc ggagctcgag aaggcgctcg aggaggccaa cagcaaggcg
     1981 ctgtacgccg ccgcggagac ccagaacgtc cgccgccggc tcgaggccga gaaggtgcag
     2041 gcggcaaatt acgcctcgag cgcgtttgcc cgcgacatgc tggcgatcaa ggatcacctc
     2101 gaccgggcgc tggccgcggt gtccgacgag cttcgcgagg acaaggtcgc gagcaatttc
     2161 ctcgacggga tcgaggcgac cgcgcgcgag ctcgaggcgg cgttcgcgcg ccagggcatc
     2221 acccgcatca agtcgatcgg cgagaagctc gacccgcacc gccaccaggc gatgatggaa
     2281 atcccgaccg acaacgccga gcccggcacc atcgtcgacg agatgcaggc cggctacatg
     2341 ctcaaggacc ggctgctccg gccggcgctg gtcggggtgg cgaaagcacc ctgaggccca
     2401 tcggccaggt cagaacggga aatacatccg gatggcgatg atgccgacca cccaggtgat
     2461 gaggttgagc gggatcccga ccttgacgaa atcgaggtaa ttgtagccgc ccatctggta
     2521 gacgatgacg ttggtctggt agccgaacgg agttgcgaac gccgcgcttc cggcgagcat
     2581 gatggcgacg aggaacggcc gcgggctgac ggcgaacgcc tcggccatcg acacggcgat
     2641 cggggtgaac agcaccgcca ccgttgcgtt cgacaggatt tccgtggcga atagcgtcgt
     2701 cccgtagaga atgaccagcg cgagcagcgg gttcatgcct tccatcgagc cgattagtgc
     2761 ggaggtcgcg gcgttggcga gtccggtctc atcgagcgcg ataccgagta cgaccatgcc
     2821 ggcgatcagc atcagcacct cgggccggag gccgccgtag gcctcgttgg cggtaatgac
     2881 ccgcagcagg atcagtagca ccgccccggc gaacgcgcag gcggcgatcg gggcgacgtt
     2941 gagcgcggcg agagtcacga ccgcgacgaa caccagcgac gaggccaggg caggccaggg
     3001 ccgcagcgca acctgccttg cgaacggctg tgccgaaccg atcaggcccc cggccagccc
     3061 cggctcgcga aaattctcgg ccgcttgggc cgcttcgcgc accccggctc gatcccgatc
     3121 ctctcccgag ccgatcagtc gagtgctgaa cagcatcagg tgcaggccgc cggcggccgc
     3181 caccatcagg ccggcgggcg tgatctcaaa gatgccgaac cgcggctggc cggcgaccat
     3241 cgccatgtcg ttgaccaaca ggttggtcga agtgccgatc agcgtgcagc agccgccgag
     3301 caccgccgcg taggacaagg ggatcaaata gcgcttcggc gagcggccga gcgagttggc
     3361 gacgtcgcgc acgaccgggg cgctgagcac gacgatcggc gtgttgttga ggaaggcgga
     3421 cgcggacccg gccaggccga tcaggatcca caggccggcc gtcccgatcc ggcggcacag
     3481 ggcgacgacc tttcggatcg ccgtatcgag gagccccgac aattccatcg cgtaggcaat
     3541 gacgaacagg gaggcgaggg cgatgatcgc cgggctggcg aatgcggcct gcacctccac
     3601 tggacggacg acgccggtca tcagaaggaa ggcggcgccg gtcagcgcga cgacatccga
     3661 ccgcaccttg tcccagatga gcgcagcgac gacggccgcc agcaccagca gggtgagcac
     3721 ttgctcggcg gtcatccgcc caattccgtt cctcctgcgc gccgccactc ctatgggcag
     3781 ttttccaagg gcggccaagc actttggctc gcgcaacggc ggagctgtgc taaggatgcc
     3841 gtcaatgacc gaattgcggc caccaccgct gcggccggcc ggctcggcga gcctgttggg
     3901 ggcgttggcg atgctcgccg cctgccagcc ggaggcgccc gacgccaatc tccccaaaaa
     3961 ggtcgagcag cccgtaccgg accttcctgc cgtcccccgg ccccagccgc cgatcgatcg
     4021 cgcgggaatc ctcgcggcgg tcgcccaggc cgccagcgcg gcggcttcgg gggccgatga
     4081 cggagcggct cagggggcgc tcgacggacg ccagttcgaa ttccgaatcc ggttcggctg
     4141 ccggggcccg tcgagcaagc tgcgcgacga atggctcggc tggtcgcacg acccggagag
     4201 cggcactttg agggtgcgcg cgatgccgac tctgtcggcg caggaagagc tggccaggga
     4261 gctgggcggc gaccggttcg agaccgtcga gggtttctgg atcccgcggc catggct
//
LOCUS       DASTZO010000076         3554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_30195,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000076 DASTZO010000000
VERSION     DASTZO010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3554
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30195"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..411
                     /locus_tag="VFU20_01990"
     CDS             67..411
                     /locus_tag="VFU20_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285264.1"
                     /translation="MNENLPIILDALPWVTAILVVSVGGWVLTTWLKIRNGYPLETSW
                     GVPLHPTTDREAAERIKLLTTENAQLRAEMGSLKDRLENIERIVTDQPAKLAREIDAL
                     AIDRAIDRGGRA"
     gene            408..713
                     /locus_tag="VFU20_01995"
     CDS             408..713
                     /locus_tag="VFU20_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285265.1"
                     /translation="MMGPGEDFIAFFVIGLPMITFGLIFNRWFKLKEKKLEVEAGLAV
                     EKAAQYAASNAELEARVRVLEKIVTDGGMQTAAAIEALRERPEETRQSRQLETDRSS"
     gene            713..958
                     /locus_tag="VFU20_02000"
     CDS             713..958
                     /locus_tag="VFU20_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285266.1"
                     /translation="MGRYEFIIAIVAIVMFASVLKARYGWQRRRHDPDSEAGDAGEAA
                     RLREEVKMLKERIHVLERITTDRENSLSREIEELRDR"
     gene            1009..1416
                     /locus_tag="VFU20_02005"
     CDS             1009..1416
                     /locus_tag="VFU20_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HEU5285267.1"
                     /translation="MPSLDDIFEDYELLDSEDRYRLLIDLGRKLEPMPDALKTDATKV
                     RGCAAAVWVYPQRTADGRLHFLADSNAAITKGIVALVLAAVQDRPAEEVAGMDVAAAL
                     APFDLSRQLSANRTQGIPSMIALVRDTAHRLAA"
     gene            1413..1853
                     /locus_tag="VFU20_02010"
     CDS             1413..1853
                     /locus_tag="VFU20_02010"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU5285268.1"
                     /translation="MSDSPAAADSERFDPERFFKVARRVGHGRALGMEYGASGDNWTE
                     LKLPWREELVGVPESGILASGAIVSLIDTASGTAVWATIGKFVPLVTLDLRIDYLRPA
                     FRGESVVARCECTKLTRRIAFTRGTAFVDDKPIAYSSGTFMIGA"
     gene            complement(1850..2386)
                     /locus_tag="VFU20_02015"
     CDS             complement(1850..2386)
                     /locus_tag="VFU20_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HEU5285269.1"
                     /translation="MGRQKRSDDWGFPRWRPYGKGRDTARVRSCDRDGCDAPGDRPAP
                     KAPNSRERWYFCEAHAAEYNRNWDYFAGLDAKAAADREAQESGEASAFRQAQHWQWAG
                     PGDGSRTRDEMRAFDALELDSDADFRSVKAAYRRLAKENHPDLKPGDEQAARRFQQVK
                     AAYDVLRRSEERREAKQR"
     gene            complement(2441..2836)
                     /gene="rnpB"
                     /locus_tag="VFU20_02020"
     ncRNA           complement(2441..2836)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFU20_02020"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2862..>3554)
                     /locus_tag="VFU20_02025"
     CDS             complement(2862..>3554)
                     /locus_tag="VFU20_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285270.1"
                     /translation="SLPDDGLLPYSLAILPKIDRVLVTNSPMQDPYLLSSNTYQVFRL
                     SDLKLLGTHRLDPGPTGNGNVAPEEARVAADGSVYVQTLSCGIQRITDLDTETPKAKL
                     VHKFPGDFCGVPTIVGRYLVQSVPNFHGFAVLDIGNPAKVREVSRLTIDEEFSPHWTG
                     WDTKAKRLVVTSGKKGNRLFLLQFDERTGALSIDERFRDTDGKPGISFATRAWPHGWT
                     GEGTPHGAVFSR"
BASE COUNT          567 a         1180 c         1231 g          576 t
ORIGIN      
        1 acgtgaccac cgagaaccgc tcgctcgccc gcgagatcga ccagcttcgc tagaggggat
       61 caattgatga acgagaacct gccgatcatt ctcgacgcgc tgccctgggt caccgcgatc
      121 ctcgtggtca gcgtcggcgg ctgggtgttg acgacctggc tcaagatccg gaacggctat
      181 ccgctcgaga cctcgtgggg cgtaccgctc catccgacca ccgatcgcga ggccgcggag
      241 cggatcaagc tgctgaccac cgagaacgcg cagcttcggg ccgaaatggg gtcgctcaag
      301 gaccggctcg agaatatcga gcggatcgtc accgaccagc ccgccaagct cgcccgcgag
      361 atcgacgccc tggccatcga ccgcgccatc gaccggggag gccgggcatg atgggtccgg
      421 gggaagactt catcgctttt ttcgtcatcg gcctgccgat gatcaccttc ggattgatct
      481 tcaatcgctg gttcaagttg aaggaaaaga agctcgaggt cgaagccggc ctggcggtcg
      541 agaaagccgc gcaatatgcg gccagcaacg ccgagctcga ggcgcgggtg cgggtgcttg
      601 agaagatcgt caccgacggc ggaatgcaga ccgcggccgc gatcgaggcg ctgcgcgagc
      661 ggcccgagga gacacggcaa tcgaggcagc tagagacgga tcggagctcc tgatgggccg
      721 atatgagttc atcatcgcga tcgtcgcgat cgtgatgttc gcgagcgtcc tgaaggcgcg
      781 ctacggctgg cagcgacgcc ggcacgatcc cgacagcgag gcgggcgacg ccggcgaggc
      841 ggcgcggctg cgcgaggagg tcaagatgct caaggagcgc atccacgtgc tcgagcggat
      901 caccaccgac cgcgagaaca gcctgtcgcg cgagatcgag gagcttcgcg accgctaggc
      961 cgagatgacg gcaggccccg gcgatgctag ttgtgggcga tggccgccct gccctccctg
     1021 gacgatatat tcgaggacta tgagctgctc gacagcgagg accgctatcg gctgctgatc
     1081 gatctcggcc gcaagctcga gccgatgccc gacgcgctca agaccgacgc gaccaaggtg
     1141 cgcgggtgcg ccgccgcggt gtgggtctat ccgcagcgca ccgccgacgg ccgcctgcac
     1201 ttcctcgccg acagcaacgc cgcgatcacc aagggcatcg tcgccctggt gctcgccgcg
     1261 gtccaggacc ggccggccga ggaggtcgcg ggcatggacg tcgccgcggc gctcgccccg
     1321 ttcgacctgt cgcggcagct gagcgccaac cgcacccaag gcatcccgag catgatcgcg
     1381 ctggtccgcg acaccgccca ccggctcgcc gcgtgagcga ttcgccggcg gccgcggaca
     1441 gcgagcgctt cgacccggaa cggtttttca aggtcgcgcg gcgggtcggc cacgggcggg
     1501 cgctgggcat ggaatatggc gcctcgggcg ataattggac cgagctcaag ctgccgtggc
     1561 gcgaggagct ggtcggggtg cccgagagcg gcatcctcgc ctcgggggcg atcgtcagcc
     1621 tgatcgacac cgccagcggg acggcggtgt gggcgaccat cggcaagttc gtgccgctgg
     1681 tgacgctcga cctgcggatc gactatctgc ggccggcatt tcgcggcgag agcgtcgtcg
     1741 cgcgctgcga gtgcaccaag ctcacccggc gaatcgcctt cacccgcggc accgccttcg
     1801 tcgacgacaa gccgatcgcc tacagctcgg gaaccttcat gatcggcgcc tagcgctgct
     1861 tggcttcgcg ccgctcctcg gaccgccgga gcacgtcgta ggccgccttg acctgctgga
     1921 agcgcctggc cgcctgctcg tcgccgggct tgaggtcggg atggttttcc ttggccagcc
     1981 ggcggtaggc ggccttgacc gagcggaagt cggcatcgct gtcgagctcc agggcgtcga
     2041 acgcccgcat ctcgtcgcgg gtccggctgc cgtcgccggg cccggcccat tgccagtgct
     2101 gcgcctgtcg gaacgccgac gcctcgccgc tttcctgcgc ctcgcggtcg gccgccgctt
     2161 tggcgtcgag gccggcgaaa taatcccagt tgcggttgta ctcggccgca tgggcctcgc
     2221 agaaatacca gcgctcgcgg ctgttcggcg ccttgggggc cggccggtcg ccgggcgcgt
     2281 cgcaaccgtc gcggtcgcaa ctgcgcaccc gcgccgtgtc gcgccccttg ccgtacgggc
     2341 gccagcgcgg gaatccccag tcgtcggatc tcttctggcg tcccatgcga ccccgaacct
     2401 agtggcgcgg gcggccgacc gccagtccgc cgatgaagaa gctgccagag ggcctgtaag
     2461 ccgggttctg tccatcgccg tgttgccacg gcgaatgggc ggccattcgt ctagccccgg
     2521 acttgcatcc ggggtccagc gaccaacccg ggcgacgggc cggaacaagc cccggagccg
     2581 aagctccatg tcgcccctat tcggtcttgc acccggtggg gtttaccgtg ccgcggccgt
     2641 tgccggcgcg cgcggtgcgc tcttgccgca ccctttcgac cttgccggcc cgaagaccgg
     2701 cggtgtcctt tctgtggcac tatccctgac cccagcaagc tggagccgcc gggcgttacc
     2761 cggcaccgtt gttccgtgga gcccggactt tcctcgccgg agctaaaagc cccgccgcgg
     2821 ccgcccggcc ctctggcagc gccttatata gctcaagcgc atcagcgcga gaagaccgcg
     2881 ccgtggggcg tcccttcccc ggtccaaccg tggggccagg cgcgagtggc gaagctgatg
     2941 ccaggcttgc catcagtatc gcggaaacgc tcgtcgatac tgagcgcgcc tgtgcgctca
     3001 tcgaactgga gcagaaagag gcgattgccc ttcttccccg aggtgacgac gaggcgtttc
     3061 gccttggtgt cccagccggt ccagtgcggg ctgaattcct cgtcgatggt gaggcgcgat
     3121 acctcgcgaa ccttggccgg attacctatg tcgagcaccg caaagccgtg gaagttggga
     3181 acgctctgga ccaggtagcg gccgacgatg gtcggaacgc cgcagaagtc gcccgggaat
     3241 ttgtgtacca gcttggcttt cggcgtctcg gtgtcgaggt cggtgatgcg ctggatgccg
     3301 cacgatagag tttggacgta gaccgagccg tcggcggcga cgcgcgcttc ctcgggcgcg
     3361 acattgccgt tgccggtcgg gccgggatcc aggcgatgag tgccgaggag cttgaggtcg
     3421 gagaggcgga agacctggta ggtgttgctc gatagcagat agggatcctg catcggactg
     3481 ttggtgacaa gcacgcgatc gatcttcggc aggatcgcga ggctgtaggg cagcaggccg
     3541 tcgtcgggta gcga
//
LOCUS       DASTZO010000077         5576 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_5990,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000077 DASTZO010000000
VERSION     DASTZO010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5576)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5576)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5576
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5990"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..465)
                     /locus_tag="VFU20_02030"
     CDS             complement(<1..465)
                     /locus_tag="VFU20_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5285271.1"
                     /translation="MARSHPLERYRNIGIMAHIDAGKTTTTERILYYTGKSYKIGEVH
                     EGTATMDWMEQEQERGITITSAATTCFWRAEEGKGEEHRINIIDTPGHVDFTIEVERS
                     LRVLDGAVACFDGVAGVEPQSETVWRQAEKYRVPRMCFVNKLDRTGASFERCV"
     gene            complement(531..1001)
                     /gene="rpsG"
                     /locus_tag="VFU20_02035"
     CDS             complement(531..1001)
                     /gene="rpsG"
                     /locus_tag="VFU20_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU5285272.1"
                     /translation="MSRRRRPEKREILPDPKFGDEVLSKFMNSVMFDGKKSVAEGIVY
                     GALESVEARLKKDPLQVFHDALNNVKPGIEVRSRRVGGATYQVPVEVRPERAQALAIR
                     WLISAARARSEKTMSARLSGELMDASQNRGNAVKKREDTHRMAEANRAFSHYRW"
     gene            complement(1062..1433)
                     /gene="rpsL"
                     /locus_tag="VFU20_02040"
     CDS             complement(1062..1433)
                     /gene="rpsL"
                     /locus_tag="VFU20_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU5285273.1"
                     /translation="MPTINQLIRKGREPQKAKSKVPAMEQNPQKRGVCTRVYTTTPKK
                     PNSALRKVAKVRLTNQREVISYIPGEGHNLQEHSVVLIRGGRVRDLPGVRYHVLRGVL
                     DTQGVKDRRQSRSKYGAKRPK"
     gene            complement(1650..2933)
                     /locus_tag="VFU20_02045"
     CDS             complement(1650..2933)
                     /locus_tag="VFU20_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5285274.1"
                     /translation="MIAAALAASAASSALAAPTYIHAGRLIAVPGKPVRGPSTIVVDN
                     GRIVAVQDGHTAPPAGAQAVDLRDRTVMPGLIDSHVHLDSDRGGEQELLAEVRDDPAL
                     HAFEAQMNGMKVLRAGFTTVRNLGDDKAVTLALRDAVRRGWVMGPRIVDAAESISTTG
                     GHMDGRGALNDQLVARLPERENLCDSVESCRRVVRRQIDRGADVIKFATTGGVNSGTG
                     LATRMNFDEAKALVETAHAYGRKVAVHAHGADGIKMALRAGADSIEHGTVMDDEVLRL
                     FRQTGAYYVPTLSTVNGYLERLAKDPNAYTPEVRKQIDWRIGITGQSLVKAVPAGVKI
                     AFGTDAGVSKHGRNADEFELMVKYGMTPLEAVKAATVNAADLLGLSSEIGTIEPGKSA
                     DLIAVAGDPLTDVTVLKRVDFIMARGEVVKAPAPR"
     gene            complement(2983..3387)
                     /locus_tag="VFU20_02050"
     CDS             complement(2983..3387)
                     /locus_tag="VFU20_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5285275.1"
                     /translation="MMRGFCDDIEKLTEANEDFRRVLYTGKHLQLVLMTLQPGEEIGE
                     EVHEDRDQFFRIEEGEGAIDIDGRQNRVEDDFAVIVPAGARHNVRNIGSEPLRFYTLY
                     GPPEHKDGIVQATKAEAEERHHAEEWDGGTTE"
     gene            complement(3461..4045)
                     /locus_tag="VFU20_02055"
     CDS             complement(3461..4045)
                     /locus_tag="VFU20_02055"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HEU5285276.1"
                     /translation="MRIVKLTVAALALAAAACADQDAPSDVSTDVNLAEDTAANDVLG
                     ANMANATASMPTDAAGFANAVAAADLYEVESARLAADKAASADVKAFAEHIRADHEKS
                     TAELKSAAGTAEITVAPKLDSEQQAMLDRLKAAGGAEFDRLFLEQQKTAHQKALALLQ
                     GYSGSGDNEALKAFAGKTAAAVKGHLDHANSIKL"
     gene            complement(4156..4614)
                     /locus_tag="VFU20_02060"
     CDS             complement(4156..4614)
                     /locus_tag="VFU20_02060"
                     /inference="COORDINATES: protein motif:HMM:NF015596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285277.1"
                     /translation="MRTPTILLSAATLGLSGCETVEPLISPPAATMVTSSSKASFGAY
                     LVDGSGRSLYILEGTRMTNGTDRCSGECLMAWPPLLVDGPPVASAGIDPARLATVPTL
                     TRPQAAYAGWPLYYYHRDQVAGDTTGQHVTDQWGTWHLLAPSGEPIRPRP"
     gene            complement(4695..>5576)
                     /locus_tag="VFU20_02065"
     CDS             complement(4695..>5576)
                     /locus_tag="VFU20_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5285278.1"
                     /translation="DVGLKQAIDNGYALGPRIVPAGYALGATGGHCDSTFLPPSLEKE
                     DKEEGIGDGPEELRHQVRRQRKFGAEVIKVCATGGVFSRNTEPGQQQLTEAELRAIAD
                     EAHQWGLRVAAHAHGAEGIKAAIRAGIDTIEHASLADDEGIRLAVARKQPVWFAMDIY
                     NTEYTQAEGKKNGVLEDNLRKDREIAQIQRDNFRKAHRAGVRQVFGSDAGVMPHGQVG
                     RQFRTMVDYGMTPLEAIRAATRNAAEALGRTRDVGALAPGRFGDIVAVSGDPLRDVSV
                     LERPDAVIKGGRLVPRS"
BASE COUNT          738 a         1876 c         1941 g         1021 t
ORIGIN      
        1 gacgcagcgc tcgaacgaag caccggtgcg gtccagcttg ttgacgaagc acatgcgcgg
       61 aacgcgatac ttctcggcct ggcgccaaac ggtttccgac tgcggctcaa cgccggcaac
      121 gccgtcgaag catgcaaccg cgccgtcgag cacgcgcagc gaacgctcga cttcgatggt
      181 gaagtcgacg tggccgggcg tgtcgatgat gttgatccgg tgctcttcgc ccttgccctc
      241 ctcggcccgc cagaagcagg tcgtcgccgc cgaggtgatg gtgatcccgc gctcctgctc
      301 ctgctccatc cagtccatgg tcgcggtgcc ctcgtggacc tcgccgatct tgtaggactt
      361 gccggtgtaa tagaggatgc gctcggtcgt cgtcgtcttg ccggcgtcga tatgggccat
      421 gatgccgatg ttgcgatagc gttcgagcgg atggctgcgg gccatttgtc agttttcctt
      481 agcgatgtgg ggagccggcg gacggcgcct ccacgatata ggtatgaaac ttaccagcgg
      541 tagtggctga aagcgcggtt ggcctcggcc atgcggtgcg tgtcctcgcg cttcttgacc
      601 gcgttgccgc ggttctgcga cgcgtccatc agctcgcccg acaggcgcgc cgacatcgtc
      661 ttttccgagc gggcgcgggc cgccgagatc agccagcgga tggccagcgc ctgggcgcgc
      721 tccgggcgca cctcgaccgg cacctggtag gtcgccccgc cgacgcggcg gctgcggacc
      781 tcgatgcccg gcttcacatt gttcaaggcg tcgtggaaga cctgcagcgg gtccttcttg
      841 aggcgcgcct cgacgctctc gagggcgccg tagacgatgc cttcggcgac cgacttcttg
      901 ccgtcgaaca tcaccgaatt catgaacttg gacagcacct cgtccccgaa cttcgggtcg
      961 ggcaggattt cgcgcttctc tgggcggcgg cgacgggaca tgtgagcttc ctttctcttc
     1021 agccggatcc ggaacccgtc cggggcttac tgctcgggtg attatttggg gcgcttggcg
     1081 ccgtacttgg agcgggactg gcggcggtcc ttgaccccct gggtgtcgag gactccgcgg
     1141 agcacgtggt agcgcactcc gggcaggtcg cggacgcggc cgccgcggat cagcacgacg
     1201 ctgtgctcct ggaggttgtg gccctcgccg gggatgtagc tgatcacctc gcgctggttg
     1261 gtcaggcgga ccttggccac cttgcgcagc gccgagttcg gcttcttcgg ggtcgtcgta
     1321 tagacccggg tgcagactcc gcgcttctgc gggttctgct ccatcgcagg gaccttggac
     1381 ttggccttct gcggctcgcg acccttgcgg atcagctggt tgatcgtcgg catgaagcct
     1441 tcaccttatg ttaccagaaa acctggcggg ttactttgcc ccacccccgc acaaatgcgc
     1501 gaaatgcgcg aagcccgggc gcccttgcgg ccgcctggcc atcgcgtcgt ggagcaatgt
     1561 tcagctcttg cccgccggag gaacgcgaat tcgctgcccc cgcggacgag cggcccaata
     1621 gcgggcgagc gggcaagggt caacggggct cagcggggag cgggcgcttt caccacctcg
     1681 ccgcgggcca tgatgaagtc gacgcgcttc aatacggtga cgtccgtgag cgggtcgccg
     1741 gcgaccgcga tgaggtcggc gctcttgccc ggctcgatgg tgccgatctc cgacgaaaga
     1801 ccgagcaaat cggcggcgtt gacggtcgcc gccttcaccg cctcgagagg cgtcatgccg
     1861 tatttgacca tcagttcgaa ctcgtcggca ttgcggccgt gcttggagac gccggcgtcg
     1921 gtaccgaagg cgatcttgac cccggccggc accgccttga ccagcgactg gccggtaatc
     1981 ccgatgcgcc agtcgatctg cttgcggacc tcgggcgtgt aggcgttggg gtccttggcc
     2041 agccgctcga gatagccgtt cacggtcgac agggtcggca cgtaataggc gccggtctgg
     2101 cggaacagcc gcagcacctc gtcgtccatc accgttccgt gctcgatcga atcggcgccg
     2161 gcccggagcg ccatcttgat tccgtcggcg ccatgcgcgt ggaccgcgac cttgcggccg
     2221 taggcgtggg cggtctcgac cagcgccttc gcctcgtcga aattcatccg ggtggcgagc
     2281 ccggtgccac tgttgacccc gccggtagtg gcgaacttga tgacgtcggc gccgcggtcg
     2341 atctggcggc ggaccacccg gcggcaggat tcgacgctgt cgcacaaatt ctcgcgctcg
     2401 ggcagccgcg ccaccagctg gtcgttgagc gccccgcggc cgtccatgtg gccgccggtg
     2461 gtcgagatgg attcggcggc atcgacgatc cgcggcccca tcacccagcc gcgccggacg
     2521 gcgtcgcgca gcgccagggt gacggccttg tcgtcgccga ggttgcggac ggtggtgaag
     2581 ccggcccgaa ggaccttcat cccgttcatc tgcgcctcga aggcgtggag cgcgggatcg
     2641 tcgcggacct cggccagcag ttcctgctcg ccgccgcggt cgctgtcgag atggacgtgg
     2701 ctgtcgatca ggccgggcat cacggtgcgg tcgcgaaggt cgactgcctg cgcgcccgcg
     2761 ggcggggcgg tgtggccgtc ctggaccgcg acgatgcgcc cattgtcgac gacgatggtc
     2821 gatggcccgc gcaccggctt gccgggtacg gcgatcagcc ggccggcgtg gatgtaggtc
     2881 ggggcggcga gcgccgagga agctgcactg gcggcaagtg ccgcggcgat catggctttt
     2941 ccaatcatgc gcccagatta agccggtgct gctcgggcgc aactattccg ttgtcccgcc
     3001 gtcccattct tcggcgtggt ggcgctcttc ggcctcggcc ttggtcgcct ggacgattcc
     3061 gtccttgtgc tcgggcgggc cgtagagcgt gtagaagcgc agcggctcgc tgccgatgtt
     3121 gcggacgttg tggcgggcgc cggctggcac gatcaccgcg aaatcgtcct cgacgcggtt
     3181 ctggcggccg tcgatgtcga tcgctccctc gccttcctcg attcggaaga actggtcgcg
     3241 gtcctcgtgg acctcctcgc cgatctcctc gccgggctgc agcgtcatca gcaccagctg
     3301 caggtgcttg ccggtgtaga gcacgcggcg aaagtcctcg ttcgcctcgg tgagcttttc
     3361 gatatcgtcg caaaagccgc gcatcatcgt ctgcccttcg cttgcaggaa gagccgtaac
     3421 gcggcgcccc gcgctacggc tccatcctcg cagcgatcct ctacagcttg atgctgttgg
     3481 cgtggtcgag gtggcccttg accgccgccg cggtcttgcc cgcgaaggcc ttgagcgcct
     3541 cattgtcgcc gctgcccgaa tagccctgga gcagcgccag cgccttctgg tgggcggtct
     3601 tctgctgctc gaggaacagc cggtcgaact ccgcgccgcc cgccgccttc agccggtcga
     3661 gcatggcctg ctgctcggag tcgagcttgg gggcgacggt gatctcggcg gtgccggccg
     3721 cgctcttgag ctcggcggtc gatttctcat ggtcggcgcg gatgtgctcg gcgaacgcct
     3781 tgacgtcggc gctggccgcc ttgtcggccg cgagccgggc gctctcgacc tcgtagaggt
     3841 cggcggcggc aaccgcattg gcgaagcccg cggcgtcggt cggcatggac gcggtggcgt
     3901 tggccatgtt ggcgccgagc acgtcgttgg cggccgtgtc ctcggcgagg ttgacgtcgg
     3961 tcgagacgtc gctgggagcg tcctggtcgg cacaggcggc cgcggcgagc gccagggcgg
     4021 ccacggtcaa tttcacgata cgcatgactg gtcttccctt accctttcaa agcggccttc
     4081 ggggccgctc ggtcgggcga aaacactttg taagtcagtg cgttccgcga tccgttcagg
     4141 cgattcgacg gaccgtcagg gccgcggccg gatcggctct cccgacggcg ccagcaggtg
     4201 ccaggtgccc cactggtcgg tcacgtgctg gccggtggtg tcgccggcga cctggtcgcg
     4261 gtggtaataa taaagcggcc agccggcgta ggcggcctgc gggcgggtca gggtcggcac
     4321 cgtcgcgagc ctcgcggggt cgatcccggc gctggcgacc ggcggcccgt cgaccagcag
     4381 cggcggccag gccatcaggc actcgcccga gcagcggtcg gtgccgttgg tcatccttgt
     4441 gccctcgagg atgtagagcg accggccgct gccgtcgacc agataggctc cgaacgacgc
     4501 cttgctggag ctggtgacca tcgtcgcggc gggcggggaa atcagcggct cgaccgtctc
     4561 gcatcccgac aggccgagag tcgccgcgga gagcaggatc gtgggagtcc tcatcgcaac
     4621 tcctccattt ctcgattgcc gttcaggtca acgcgacccg cccccctccg ttccgggttg
     4681 ccgggcgccg gcgctcaact gcgcgggacc agccggccgc ccttgatcac cgcgtccggc
     4741 cgctcgagca cgctgacgtc gcgcagcggg tcgccgctca ccgcgacgat gtcgccgaac
     4801 cggccgggcg ccagcgcgcc gacgtcgcgg gtgcggccga gcgcctcggc ggcgttgcgg
     4861 gtcgcggccc ggatcgcctc gagcggggtc atcccgtagt cgaccatggt ccggaactgc
     4921 cggccgacct ggccgtgcgg catcaccccg gcgtcgctgc cgaacacctg ccggaccccg
     4981 gcgcggtggg ccttgcggaa attgtcgcgc tggatctgcg cgatctcgcg gtccttgcgc
     5041 aaattgtcct cgagcactcc gttcttcttg ccctcggcct gggtgtattc ggtgttgtag
     5101 atatccatcg cgaaccacac cggctgcttt cgggcgacgg ccaggcggat gccctcgtcg
     5161 tcggccaggc tggcgtgctc gatggtgtcg atgccggcgc ggatcgccgc cttgatgccc
     5221 tcggcgccgt gggcgtgggc ggcgacccgc agcccccact ggtgcgcctc gtcggcgatc
     5281 gcccgaagct cggcctcggt cagctgctgc tggccgggct cggtgttgcg cgagaagacg
     5341 ccgccggtcg cgcagacctt gatcacctcg gcgccgaact tgcgctggcg gcggacctgg
     5401 tggcgaagct cctcgggccc gtcgccgatc ccctcctcct tgtcctcttt ctcgaggctc
     5461 ggcggaagga aggtggagtc gcagtggccg ccggtcgctc ccagagcata gccggccggg
     5521 acgatccgcg ggccgagggc atagccgttg tcgatcgcct gcttcagccc gacgtc
//
LOCUS       DASTZO010000078         6143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_919873,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000078 DASTZO010000000
VERSION     DASTZO010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6143
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919873"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFU20_02070"
     CDS             complement(<1..680)
                     /locus_tag="VFU20_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5285279.1"
                     /translation="MTKTILITGATAGIGAAAARRFAAGGWRVIATGRRSERLRALAE
                     ELGDACLPLELDVRDPATADQLARLSPPWGDIDLLLNNAGLAPPMDPLQDSELEPQLN
                     VIQTNIAGLVALTRALLPGLIERKGAVINLSSVAATYPYRGGAVYGASKAFVRQFSLD
                     LRCDLAGTGVRVTSIEPGMAETEFTLVRTGGDQEASDKLYANMNPMTADDLAETIWWV
                     ANLPPHLN"
     gene            complement(677..1291)
                     /locus_tag="VFU20_02075"
     CDS             complement(677..1291)
                     /locus_tag="VFU20_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5285280.1"
                     /translation="MSRPLLITDCDEVLLHMVAHFAEWLDEEHGVRFDVESGQFDAMT
                     DRRTGAPVPLERVWPLLDSFFEGHMHRQTLVPGALDALERIGEIADIVVLTNLGDEAH
                     GWRVDQLAGHGIRHDIVCNRGGKGRPVRDLVDARGPPVAVFVDDLAVHHSSVAKHAPG
                     VWRLHMVAEPRLAAAVPAAEDAHARIDDWPAACEWILDKFRGER"
     gene            complement(1288..1554)
                     /locus_tag="VFU20_02080"
     CDS             complement(1288..1554)
                     /locus_tag="VFU20_02080"
                     /inference="COORDINATES: protein motif:HMM:NF023518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 family protein"
                     /protein_id="HEU5285281.1"
                     /translation="MSGATTNDPSALALRALAATLSNQRLAERFLSLSGVDPPDLRQR
                     ASDPDLLAALLRFLEAHEPDLVAVAGEIGATPDALVAARRELER"
     gene            1604..2014
                     /locus_tag="VFU20_02085"
     CDS             1604..2014
                     /locus_tag="VFU20_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5285282.1"
                     /translation="MLIVEDNALNIKLFCDLLAAHGHQPQAVTDSRLALDSARTFDPD
                     LVITDIQLPHVSGLELIRLLRADAQLGEVPIMAVTAYSAQGDEERIRAAGAQSYVSKP
                     ISVVKFVAAVQELLPPHVAANGSGSAAGDPGGES"
     gene            complement(2068..2235)
                     /gene="rpmG"
                     /locus_tag="VFU20_02090"
     CDS             complement(2068..2235)
                     /gene="rpmG"
                     /locus_tag="VFU20_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU5285283.1"
                     /translation="MAKPATVKIKLVSSADTGFFYVTKKNPRNQTEKLVFKKYDPVAR
                     KHVEFKEAKIK"
     gene            2402..2710
                     /locus_tag="VFU20_02095"
     CDS             2402..2710
                     /locus_tag="VFU20_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285284.1"
                     /translation="MASDPLALVRGQIGLRIAEIQDRGPRLSPLDLYARMDAIRQLAA
                     EHGMVALEGLARCSAQRALLPGHRIAMQSCLEHVDEALDSHSPADCTTILAALAVRLH
                     "
     gene            2752..4380
                     /locus_tag="VFU20_02100"
     CDS             2752..4380
                     /locus_tag="VFU20_02100"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cisplatin damage response ATP-dependent DNA
                     ligase"
                     /protein_id="HEU5285285.1"
                     /translation="MRAFSQLLDALVYTRSRNTKLKLIGDYLRETPDPDRGIALAALT
                     GGLDIPHVKPAAIRGLIEERIDPMLFHMSRDYVGDTAETVALLWPKGVGPGEQCAEPP
                     ELDDATVGIADAVGRLRGAGRLDAPRVLAAMLDHLDASGRYALLKLATGELRVGISAR
                     LAKQALADAFGLDVDAVEEVWHGLRPPYAELFDWAEGRGAQPTPRDVPVFRPFMLAHP
                     LEDSKLSLDEFAAEWKWDGIRVQLVHVANASGGETRLYSRTGDDISGSFPDVASAFAI
                     PAVLDGELLVRGTEQGADEHGGAAASFNALQQRLGRKTVSARMQGDYPAFVRLYDILF
                     EGDQDVRGLPWTERRARLERFAGRLDPERFDLSQLIEAASFDELEKLRAGARDAAIEG
                     MMLKRRDAPYVAGRRAGLWYKWKRDPLTADCVLMYAQRGSGKRSSYYSDYTFGCWTED
                     GELLPVGKAYFGFTDEELKWLDNFVRRNTVERFGPVREVAKTLVLEVAFDSIHSSKRH
                     KSGLAMRFPRISRIRTDKPAAEADRIAALQAMVT"
     gene            complement(4388..5683)
                     /locus_tag="VFU20_02105"
     CDS             complement(4388..5683)
                     /locus_tag="VFU20_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5285286.1"
                     /translation="MASLESSIGIVTGAIAVLTTVVAVTRHWTKVQDQAERDKIAAEN
                     RDLVRRIAELESRRDQLLEQISVAGRAGSAALHQKSELDSQLQSLMGATGASGGSVYI
                     PVRSPRGEVHGLAFLCIEPFSAQTQVLKSKIIPLKSLAGRCFISGESFVVVNASQNPD
                     HFQLATEIAEYRPSTTLNMALRSGGEVVGVLQLLSREGEAGFGDDDVGRVSALIAPIA
                     DGVAAIAQSPDYLKTLGLGDDTKPVEGSVVYFDLSGSALLFQELSSSFALQLLNEYFE
                     RVCEAAFRAGATLDTYMGDGALIRFNVPRPQPEHELAAVKAALDMNRAFLEMKDYWVA
                     ISPQLVAVQHRAGIATGPLLRANLGHSQIQSLTVIGYPVSVAAALCEAADRDRTVTLV
                     SQETYEAVKDQVIASQLDRGRLGKAERFTDAAWEIVGLR"
     gene            complement(5810..>6143)
                     /locus_tag="VFU20_02110"
     CDS             complement(5810..>6143)
                     /locus_tag="VFU20_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU5285287.1"
                     /translation="NLRIEGEPMEVVRGGGGGAGGYGDRGAGGDRGGPQRQLTGEKAS
                     GTVKFFNAMKGFGFIQRDDGQPDAFVHISAVERAGMPTLNEGDRLEFEIEVDRRGKYA
                     AVNLTPVQ"
BASE COUNT          956 a         2088 c         2104 g          995 t
ORIGIN      
        1 atgttgaggt gcggcggcaa gttggcgacc caccagatgg tctcggcgag gtcgtcggcg
       61 gtcatcgggt tcatgttcgc gtagagcttg tccgacgctt cctggtcgcc gccggtgcgg
      121 accagggtga actcggtctc ggccatgccc ggctcgatcg aggtgacccg gactccggtg
      181 ccggcgaggt cgcaccgaag gtcgagcgag aactggcgga cgaacgcctt gctggcgccg
      241 tacaccgcgc cgccgcggta gggataagtc gcggcgaccg acgacaggtt gatcaccgca
      301 cccttgcgct cgatcaggcc gggaagcagc gcccgggtca gggcgaccag gccggcgatg
      361 ttggtctgga tgacgttcag ctgcggctcg agctcggaat cctgcagcgg gtccatcggc
      421 ggcgccagcc cggcattgtt gagcagcagg tcgatgtcgc cccacggcgg cgacagccgg
      481 gcgagctggt cggccgtcgc gggatcgcgg acgtcgagct cgagcggcag gcacgcgtcg
      541 cccagctcct cggccagcgc ccgcagccgc tcgctgcggc ggccggtggc gatcacccgc
      601 cagccgccgg cggcgaaacg ccgcgccgcg gccgcgccga tgccggcggt ggcgccggtg
      661 atcaggatag ttttcgtcat cgctcgcccc tgaacttgtc gagaatccac tcgcacgccg
      721 ccggccagtc gtcgatgcgc gcgtgcgcgt cttcggccgc cggcaccgcg gcggcgagcc
      781 ggggctcggc gaccatgtgc agccgccaca cgcccggcgc atgcttggcg accgagctgt
      841 gatggacggc gaggtcgtcg acgaacaccg cgaccggggg gccgcgggca tcgacgaggt
      901 cgcgcaccgg acggcccttg ccgccccggt tgcagacgat gtcgtggcgg attccgtggc
      961 cggcgagctg gtcgacgcgc cagccgtgcg cctcgtcgcc gaggttggtg aggacgacga
     1021 tgtcggcaat ctcgccgatc cgctcgagcg cgtcgagcgc tcccgggacc agggtctggc
     1081 ggtgcatgtg gccttcgaag aaggagtcga gcagcggcca tacccgctcg agcggcaccg
     1141 gagcgccggt gcggcggtcg gtcatcgcgt cgaactggcc gctttcgacg tcgaaccgga
     1201 cgccgtgctc ctcgtcgagc cactcggcga aatgcgcgac catgtgcaac agcacctcgt
     1261 cgcagtcggt gatcagcaag ggcctgctca tcgctcgagc tcgcgtcggg cggcgaccag
     1321 ggcatcgggc gtcgcaccga tctccccggc gaccgcgacc aggtcgggct cgtgcgcctc
     1381 gaggaagcgc agcagcgcgg cgagaaggtc cggatcggac gctctttgtc tcaaatcggg
     1441 aggatcgacg ccgctcaagg aaagaaagcg ctccgcgagc cgttgattgc ttagtgtcgc
     1501 cgcgagcgcc cgcagcgcaa gggccgatgg gtcatttgtc gtcgcgccgc tcatggctta
     1561 cggtccgtct ggggctcgcg agtggggaat tgagttggcc aagatcctga tcgtcgagga
     1621 caacgccctc aacatcaagc tgttctgcga cctgctcgcc gcgcacggcc accagccgca
     1681 ggcggtgacg gacagccgcc tggcgctcga ttccgcgcgc accttcgacc ccgacctggt
     1741 gatcaccgac atccagctgc cgcacgtcag cgggctcgag ctgattcgtc tgctgcgcgc
     1801 cgatgcccag ctcggcgagg tgccgatcat ggcggtcacc gcttattcgg cccagggcga
     1861 cgaggagcgg atccgggccg ccggagcgca atcctatgtg tccaagccga tctcggtggt
     1921 gaagttcgtc gccgcggtcc aggagcttct gccgccgcac gtcgccgcca acggaagcgg
     1981 cagtgcggcg ggcgatccgg gcggcgaaag ctaggctggg cgccggatcc agtccgggat
     2041 gaccaggccg gacgccgtcg cgccgcctta cttgatcttc gcttccttga actcgacgtg
     2101 cttgcgagcg accgggtcgt acttcttgaa gaccagcttc tcggtctggt tgcgcgggtt
     2161 cttcttggtc acgtagaaga agccggtgtc ggcggagctg acgagcttga tcttgacggt
     2221 tgccggcttg gccatggcgg ggtcccaata gctgaaaaca agaaaagcgg cccgcgcgcg
     2281 gcgccgctcg atcggcgcgc tccttggccg caggacgcgt ttttgtcaac cgtcgcgcca
     2341 gtgtaacaag gctttaacct tgctgtgcca acctcctgtt aacaagagga ggcctggcat
     2401 catggcaagc gatccgctgg cgctcgtgag agggcagatc gggctgcgca tcgccgaaat
     2461 ccaggatcgc ggcccgcgcc tgtcgccgct cgacctctac gcaaggatgg acgcgatccg
     2521 ccagctggcc gccgagcacg gcatggtcgc cctggagggc ctcgcgcgct gctcggccca
     2581 gcgggcgctg ctgcccggcc accggatcgc gatgcagtcc tgcctcgagc atgtcgacga
     2641 ggcgctcgac agccattcgc ccgccgactg cacgacgatc ctcgccgcgc tcgccgtccg
     2701 gctgcattag cttcccggca ccgtagcggt gcctcgcgca ttggaagcgc gatgcgcgct
     2761 ttctcccagc tgctcgacgc cctcgtctac acgcgctcgc gcaacaccaa attgaagctg
     2821 atcggcgatt atctcaggga aacgccggac ccggaccgcg ggatcgcgct cgccgcgctg
     2881 accggcgggc tggacatccc tcacgtcaag cccgcggcga tccgcggcct gatcgaagag
     2941 cggatcgacc caatgctgtt ccacatgagc cgcgactatg tcggcgacac cgccgagacg
     3001 gtggccctgc tgtggccaaa gggggtgggc ccgggggagc aatgcgccga gccccccgag
     3061 ctcgacgacg cgaccgtcgg catcgccgac gcggtcgggc ggttgcgcgg cgccggccgg
     3121 ctcgacgctc cgcgggtgct ggcggcgatg ctcgaccatc tcgatgcatc gggccgctac
     3181 gccttgctca agctggcgac cggcgagctt cgggtcggga tcagcgcgcg gctcgccaag
     3241 caggcgctgg ccgacgcgtt cgggctcgac gtcgatgcgg tcgaggaggt gtggcacggc
     3301 ctccgcccgc cctatgccga gctgttcgac tgggcggaag gccgcggcgc ccagccgacg
     3361 ccccgcgacg tgccggtgtt ccggccgttc atgctcgccc acccgctcga ggactcgaag
     3421 ctgtcgctcg acgagttcgc cgccgaatgg aagtgggacg ggatccgggt gcaattggtg
     3481 cacgttgcca atgccagcgg cggcgaaacc cggctctaca gccgcaccgg cgacgacatt
     3541 tccggcagct tccccgacgt cgcctcggcg ttcgcaattc ccgcggtgct cgacggcgag
     3601 ctgctggtgc gcgggaccga acagggcgcg gacgagcacg gcggcgccgc ggcgagcttc
     3661 aacgcgctcc agcagaggct cgggcgcaag accgtcagcg cccggatgca gggcgattat
     3721 ccggcgttcg tgcggcttta cgacatattg ttcgagggcg accaggatgt gcgggggctg
     3781 ccgtggaccg agcgtcgggc gcggctcgag cggttcgccg gccggctcga cccggagcgc
     3841 ttcgacctgt cgcagctgat cgaggcggcg agcttcgacg agctcgagaa gttgcgcgcc
     3901 ggcgcccgcg acgcggcgat cgaggggatg atgctcaagc ggcgcgacgc cccctatgtc
     3961 gccggccgcc gcgccggact gtggtacaaa tggaagcgcg acccgctgac cgccgattgc
     4021 gttttgatgt acgcgcagcg cggctcgggc aagcggtcga gctattattc cgactacacg
     4081 ttcggctgct ggaccgagga cggggagctg ctcccggtcg gcaaggccta tttcggcttc
     4141 accgacgagg aactgaagtg gctcgacaat ttcgtccgcc gcaacaccgt cgagcgcttc
     4201 gggccggtgc gcgaggtcgc caagacgctg gtgctcgaag tcgcgttcga ttcgatccat
     4261 tcgagcaagc gccacaagtc gggcctggcg atgcgcttcc cgcggatcag ccggatccgc
     4321 accgacaagc cggcggccga ggccgaccga atcgcggcgc tccaggcgat ggtgacgtaa
     4381 agcgcgtcta gcgcaggccg acgatttccc acgcggcgtc ggtgaaccgt tccgctttgc
     4441 ccaggcggcc acggtccagt tgactggcga tgacctgatc cttcacggcc tcataggtct
     4501 cctggctgac gagcgtcacc gtacggtcgc ggtccgctgc ctcgcaaagg gcggcggcaa
     4561 cggagaccgg atagccgatg accgtgaggc tctggatctg cgaatggccg aggttggccc
     4621 gcagcaatgg tcccgtggcg attccggcgc ggtgctggac cgcgacgagt tgagggctga
     4681 tggccaccca atagtccttc atctccagga acgcgcggtt catgtcgagc gccgccttca
     4741 ccgccgcgag ctcgtgttcc ggttgcggcc gggggacgtt gaaccggatg agcgccccat
     4801 cccccatgta cgtgtccagc gttgcgccgg cgcggaacgc ggcttcgcaa acgcgttcaa
     4861 aatattcgtt cagcaactgg agcgcgaaac tcgaggaaag ctcctggaac aacagggcgg
     4921 acccggaaag gtcgaaatag acgaccgagc cctccaccgg cttggtgtca tcgccgagcc
     4981 cgagtgtctt caaatagtcc gggctctgcg cgatcgccgc cactccgtcc gcaatcgggg
     5041 cgatcaacgc ggagacccgg ccgacgtcgt cgtcgccaaa gccggcctcc ccttcccggc
     5101 tgagcagttg aagcacgccg acgacctcgc cgccgctgcg cagcgccatg ttcagggtcg
     5161 tcgacggccg atactccgca atctcggtcg ccagctgaaa atgatcggga ttctgcgatg
     5221 cgttgaccac cacgaacgat tccccgctga tgaaacagcg tcccgcgaga ctcttgagcg
     5281 ggatgatctt tgatttcaga acctgggttt gcgcgctgaa cggctcgatg cacaaaaatg
     5341 ccagcccatg cacttcgccg cgtgggctgc ggaccgggat atagaccgag ccgccgctcg
     5401 ccccggttgc ccccatcagc gactgaagtt gcgagtcgag ttccgatttc tggtgaagcg
     5461 ccgcgctgcc ggctcgaccg gcgaccgaaa tctgctccag caattggtcg cgacgcgact
     5521 cgagctcggc gatcctgcgg accaggtcgc gattctccgc tgcgatcttg tctcgctcgg
     5581 cctggtcctg gaccttggtc cagtgacgcg tgacggccac gaccgtggtg agaaccgcga
     5641 tggccccggt gacaattccg atagagctct cgaggcttgc cattcttccc cccggcaggc
     5701 gccctgcgac attacgctag tggcgcgggc gatcaagcgg cgcggtcgaa caagaaagcg
     5761 cccgcccggc gatatggccg gacgggcgct tgccccgctc ttaactcgct tactggaccg
     5821 gcgtcaggtt caccgccgca tatttgccgc ggcggtcgac ctcgatctcg aactccagcc
     5881 ggtcgccctc gttcagggtc ggcatcccgg cgcgctcgac ggccgagatg tggacgaacg
     5941 catcgggctg cccgtcgtcg cgctggatga agccgaagcc cttcatcgcg ttgaagaact
     6001 tgaccgttcc gctggctttc tcgccggtca actggcgctg cgggccgccg cggtcgccgc
     6061 cggcgccgcg atccccgtag ccgcctgcgc cgccgccgcc gccgcgcacg acctccatcg
     6121 gctcgccctc gatccgaagg ttg
//
LOCUS       DASTZO010000079         5760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_762671,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000079 DASTZO010000000
VERSION     DASTZO010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5760
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762671"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(87..824)
                     /locus_tag="VFU20_02115"
     CDS             complement(87..824)
                     /locus_tag="VFU20_02115"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HEU5285288.1"
                     /translation="MPALEKPDSPLTTSVKWRYPAVHPEGRKFLVIAATATFFAWLLD
                     WEFLTFLLVGLTIWVAAFFRDPVRTTPRGANLVVAPADGLITMIQRVAPPAELAGPDG
                     LGDAHYTRVSIFMSVFDVHINRSPITGRIRRIAYVPGKFLNADLDKASEDNERQHFIV
                     EGADGVKVGFTQIAGLVARRILSFVREGDSVEAGQRVGLIRFGSRVDVYLPQGTDTKV
                     LLGQTVIAGETILAELGRQALIEGVRQ"
     gene            932..2176
                     /locus_tag="VFU20_02120"
     CDS             932..2176
                     /locus_tag="VFU20_02120"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HEU5285289.1"
                     /translation="MAKIKVKTPLVELDGDEMTRIIWKWIRERLIQPYLDVELIYYDL
                     SIQKRDETEDRITVEAAEAIRKYGVGVKCATITPDEARVEEFKLKKMWKSPNGTIRNI
                     LNGVVFREPIVIANVPRLIPGWTDPIVVGRHAFGDQYKATDFKVPGAGKLTIRWEAAD
                     GSSSIEQEVFDFPAAGVAMGMYNLDESIRAFARASFNYGLNRGWPVYLSTKNTILKAY
                     DGRFKDIFEEVFESEFKDKFAEAKIEYQHRLIDDMVASALKWSGKFVWACKNYDGDVQ
                     SDLVAQGFGSLGLMTSVLMTPDGKTIEAEAAHGTVTRHYRMHQQGKATSTNPIASIFA
                     WTGGLKYRGKFDDTPEVVRFAETLERVCVATVESGKMTKDLAILVGPEQPWMTTEQFF
                     EAIRENLEAAMAQPAEAEPQAT"
     gene            complement(2136..3350)
                     /locus_tag="VFU20_02125"
     CDS             complement(2136..3350)
                     /locus_tag="VFU20_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5285290.1"
                     /translation="MTSPDDLIVVGGGPAGMMAGLLFARAGCTVRVLEKHADFFRDFR
                     GDTVHPSTMEILDQLGMLERFLKRPHNRIDNARLNLAGRDWTIGDLSHLDTAAPFIAM
                     MPQWDFLDFLRDEAHAFPGFRLDMESPVERFIEEGGRVAGVRLAGGRELRASLTIAAD
                     GRSSIVRAMLPVETLGAPMDVLWFRLPKADDRVEGLRGRVERGRLLVLIDRGDYWQCA
                     LLIPKGGADEFKARGLDYIRSIVAAAAPDLDLSELDELGDLHLLSVNLDRLMCWHRPG
                     LLAIGDAAHAMSPIGGIGINLAIQDAVAAANVLAGPLARGEDVDPLLGRVEKRRLFPT
                     RLIQAAQKAAQDRVIGRLLLPGEPIRKVPLAVRLLDRFPLLRRIPGRLIGLGVRRERV
                     RSPVARPRPAAP"
     gene            3436..5226
                     /locus_tag="VFU20_02130"
     CDS             3436..5226
                     /locus_tag="VFU20_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5285291.1"
                     /translation="MATEFQTSGLSDALVILGAAGLVIPAFARIRITPVIGFLLIGLV
                     VGPAGLGAMTGRYPWLHYVTISNPEAIRPFAELGVILVLFSIGLELSFRRLWSMRRLV
                     FVVGAAELLVSAAIIALGLHLIGQGLAGAIGLGLALALSSTAIVLPLVGTHSPVGRSA
                     LAMLLFEDLAIVPIVFALGAMAPYAAGNGFGDLLTTMLLGGAAAVAMLFAGRLLLPPL
                     FAQAARTKSPELFLAACLLVVIVASLATAAIGFSPILGALLAGIVIAETEYRGEVEVM
                     TAPFRGLGLGIFLITVGMSVDLRLVAANWQDIGLAVIGVLAVKAAVTAALLRIEGART
                     SVAAETGVLMASPSETTLIVLSAAAAAQLIQPQTAAFWQIVTAIGLTITPLLAGLGRL
                     AGRGVDSQTRSELTGALEEGDTPKVVILGFGRVGRMVGEMLDAHAKPYVAVDSDVDCV
                     ASARREQLPVVFGDVAHPELIDRLRPSYPAAFILTMDNPVLAKRLARRIRSAFPDLPI
                     VARARDPAHAAELYKAGVTDAVPETLEASLQLSEAALVDLGVAMGPVIASIHEKRAQL
                     QAEIKEEAELEEEPRLGRRRLRDAGPQTSL"
     gene            complement(5211..>5760)
                     /locus_tag="VFU20_02135"
     CDS             complement(5211..>5760)
                     /locus_tag="VFU20_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285292.1"
                     /translation="SAERGPRRPGKRGGVMTKSPQSIEENSEEVAGYVYQEPLIIRMI
                     SIVGWLLGWATYWLARWGFSEKAEALIDDAIVMRRANPGAIRAIGYLNGLCGLPAIRR
                     GRLIIHGKIHETGPFANKGFFATYDVAAPNKARALALIKRFEWDAIPESLEVEEGELE
                     YPDPGAEGVLWVAPGRSFYSEV"
BASE COUNT          935 a         1931 c         1970 g          924 t
ORIGIN      
        1 cggaacctcg ttctcgctcg ccttggggcc gagccacgac ggaccaggag tggccggctc
       61 gtcgccgtcc gggtccgcga cgtcgctcac tggcggacgc cctcgatcag cgcctgcctg
      121 ccgagttcgg cgagaatcgt ctcgccggcg atcacagtct ggccgagcag gaccttggtg
      181 tcggtgcctt gcggcaggta gacgtcgacc cggctgccga agcggatcag cccgacgcgc
      241 tgccccgcct cgacgctgtc gccctcgcgg acgaacgaca ggatccggcg cgcgaccagg
      301 ccggcgatct gggtgaagcc gaccttcacc ccgtcggcgc cctcgacgat gaaatgctgc
      361 cgctcattgt cctcgctcgc cttgtcgagg tcggcattga ggaacttgcc gggcacgtag
      421 gcgattcggc ggatccggcc ggtgatcggc gagcgattga tgtggacgtc gaacacgctc
      481 atgaaaatcg agacacgggt gtagtgcgcg tcgccgagcc cgtcggggcc ggcgagctcg
      541 gccggcgggg cgaccctctg gatcatggtg atcaacccgt cggcgggcgc gacgacgagg
      601 ttggcgccgc gcggcgtcgt gcgcaccggg tcgcggaaga aggccgcgac ccagatcgtc
      661 agcccgacca gcaggaaggt gaggaattcc cagtcgagca gccaggcgaa gaaggtcgcc
      721 gtggccgcga tgacgaggaa cttgcgcccc tcgggatgaa ccgccggata gcgccacttg
      781 acgctcgtgg tgagcgggct gtcgggcttt tcgagcgcgg gcattgcatc ccccttagta
      841 gcggcgtccc gcgcgcacaa cgccgcgcgg ttgcctcgac ttttggcgca gcctaaggcg
      901 ccgcggaccg cctgaggaaa cagggaagag catggccaag atcaaggtga agaccccgct
      961 cgtcgagctc gacggcgacg agatgacccg catcatctgg aagtggatcc gcgagcggct
     1021 gatccagccc tatctcgacg tcgagctgat ctattacgac ctgtcgatcc agaagcgcga
     1081 cgagaccgag gaccggatca cggtcgaggc ggccgaggcg atcaggaaat acggcgtcgg
     1141 ggtcaaatgc gcgacgatca cgcccgacga ggcgcgggtc gaggaattca agctcaagaa
     1201 gatgtggaag tcgcccaacg gcaccatccg caacatcctg aacggggtcg tcttccgcga
     1261 gccgatcgtg atcgccaacg tgccgcggct gatccccggc tggaccgacc cgatcgtcgt
     1321 cggccgccac gcgtttggcg accagtacaa ggcgaccgac ttcaaggtgc ccggcgccgg
     1381 caagctgacc atccggtggg aagccgccga tggcagcagc tcgatcgagc aagaggtgtt
     1441 cgacttcccc gccgccgggg tcgcgatggg catgtacaac ctcgacgaat cgatccgggc
     1501 gttcgcccgg gccagcttca actatggatt gaaccgcggc tggccggtct atctgtcgac
     1561 caagaacacc atcctcaagg cctatgacgg ccgcttcaag gatatcttcg aggaagtttt
     1621 cgaaagcgaa ttcaaggaca agttcgccga agcgaagatc gagtatcagc accgtctgat
     1681 cgacgacatg gtcgcctccg ccttgaagtg gagcggcaag ttcgtctggg cgtgcaagaa
     1741 ttacgacggc gacgtgcagt ccgacctggt cgcgcagggt ttcggcagcc tcgggctgat
     1801 gacctcggtg ctgatgaccc ccgacggcaa gaccatcgag gcggaggccg cccacggcac
     1861 cgtcacccgc cattaccgga tgcatcagca gggcaaggcg acctcgacca acccgatcgc
     1921 ctcgatcttc gcctggaccg gcggcctcaa gtaccgcggc aagttcgacg acacccccga
     1981 ggtcgtccgc ttcgccgaaa cgctcgagcg ggtgtgcgtc gcgaccgtcg agagcggcaa
     2041 gatgaccaag gacctcgcca tcctcgtcgg ccccgagcag ccgtggatga ccaccgagca
     2101 attcttcgag gcgatccgcg agaacctcga agcggctatg gcgcagccgg ccgaggccga
     2161 gccacaggcg acctgacccg ctcgcggcgg acgccgaggc cgatcagtcg gccggggatt
     2221 cgccgcagca gcgggaagcg gtcgagcagg cggacggcca gcggcacctt gcggatcggc
     2281 tcgccgggca ggagcaggcg gccgatgacc cggtcctgcg ccgctttctg agccgcctgg
     2341 atcagccgcg tcgggaacag gcgccgcttc tcgacccggc cgagcagcgg gtcgacgtcc
     2401 tcgccgcggg ccagcggtcc ggctaggacg ttcgccgcgg cgaccgcgtc ctggatcgca
     2461 aggttgatgc cgatgccgcc gatcggcgac atggcgtgcg ccgcatcgcc gatcgccagc
     2521 agccccggcc ggtgccagca catcagccgg tcgaggttga ccgaaagcag atgcaggtcg
     2581 ccgagctcgt cgagctcgct caggtcaagg tcgggcgctg ccgccgcgac gatcgagcgg
     2641 atgtaatcga ggcccctggc cttgaactcg tcggcgccgc ccttggggat cagcaaggcg
     2701 cactgccagt agtcgccgcg gtcgatgagg accaggagcc ggccgcgctc gacccgcccg
     2761 cgcaagccct cgacgcggtc gtcggccttg gggaggcgga accacagcac gtccatcggc
     2821 gcgcctagcg tctcgaccgg gagcatggcg cggacgatcg acgagcggcc gtcggcggcg
     2881 atggtcagcg aggcgcgaag ctcgcggccg ccggcgagcc gcactccggc cacccgtccg
     2941 ccctcctcga taaagcgttc gacggggctt tccatgtcca gtcggaagcc gggaaaggcg
     3001 tgggcttcgt cgcgcaggaa atcgaggaag tcccattgcg gcatcatggc gatgaacggc
     3061 gccgcggtgt cgaggtgcga caggtcgccg atggtccagt cgcggccggc gaggttgagc
     3121 cgcgcattgt cgatccggtt gtgcggccgc ttcaggaacc gctcgagcat ccccaactgg
     3181 tcgaggatct ccatggtcga gggatgtacg gtgtcgccgc ggaaatcgcg gaagaagtcg
     3241 gcgtgcttct ccaggacccg cacggtgcag ccggcgcggg cgaacagcag cccggccatc
     3301 atcccggccg gcccgccgcc gacgacgatg agatcgtccg gtgacgtcat gacggcgtcc
     3361 tagccggaat cgtcatcccc gcgaaatggg tgttcccgct ttcgccggaa ggacgaaatg
     3421 ggcacaagca gtgccatggc gaccgaattc cagacctccg gcctcagcga cgccttggtc
     3481 atcctcggcg ccgccgggct ggtcatcccg gctttcgcgc ggatccggat cacgccggtg
     3541 atcggcttcc tcctgatcgg gctggtggtc gggccggcgg ggctcggggc gatgaccggc
     3601 cgctatccgt ggctccacta cgtgaccatc tccaacccgg aggcgatccg gccgttcgcc
     3661 gagctgggcg tgatcctggt attgttctcg atcgggctcg agctgtcgtt ccgccgcttg
     3721 tggtcgatgc gccggctggt gttcgtggtc ggcgccgccg agctgctcgt gtccgccgcg
     3781 atcatcgcgc tcgggctgca cctgatcggc cagggcctcg ccggggcgat cggcctcggg
     3841 ctggcgctgg cgctctcgtc gaccgcgatc gttctgccgc tggtcggcac ccacagcccg
     3901 gtcggccgct cggcgctggc gatgctgctg ttcgaggacc tcgccatcgt cccgatcgtg
     3961 ttcgcattgg gggcgatggc gccctacgca gccggcaacg ggttcggcga cttgctgacg
     4021 acgatgctgc tcggcggagc ggccgcggtc gcgatgctgt tcgccggccg gctgctgctg
     4081 ccgcccctgt tcgcccaggc ggcgcggacc aagagccccg agctgttcct agccgcctgc
     4141 ctgctggtgg tgatcgtcgc cagcctggcc accgcggcga tcggcttctc gccgatcctg
     4201 ggggcgctgc tcgccggaat cgtcatcgcg gagaccgagt atcgcggcga ggtcgaggtg
     4261 atgaccgcgc cgttccgcgg gcttgggctc ggcatcttcc tgatcaccgt cgggatgagc
     4321 gtcgacctgc gcctggtcgc ggccaattgg caggacattg gcctcgccgt gatcggggta
     4381 ctggccgtca aggcggcggt caccgcggcg ctgctgcgga tcgagggcgc ccggacctcg
     4441 gtcgccgcgg aaaccggcgt gctgatggcc tcgccgtcgg aaacgaccct gatcgtcctg
     4501 tcggcggcgg ccgcagcgca actcatccag ccgcagaccg cggccttctg gcagatcgtc
     4561 accgcgatcg ggctgaccat caccccgctg ctcgccgggc tcgggcgcct cgccgggcgc
     4621 ggggtcgact cgcagaccag gtcggagctg acgggggcgc tcgaggaagg cgacacgccc
     4681 aaggtcgtga tcctcggttt cggacgcgtg ggccggatgg tcggcgagat gctcgacgcg
     4741 cacgccaagc cctatgtggc ggtggattcg gacgtcgatt gcgtcgcctc ggcccggcgc
     4801 gagcaattgc cggtggtgtt cggcgacgtc gcccaccccg agctgatcga ccggctgcgg
     4861 ccgagctacc ccgccgcctt catcctgacc atggacaatc cggtgctcgc caagcggctg
     4921 gcgcggcgca tccggagcgc cttccccgac cttcccatcg tcgcccgcgc ccgcgacccc
     4981 gcgcatgcgg ccgagctcta caaggcgggc gtgaccgacg ccgtgcccga gacgctcgag
     5041 gcgtcgctgc agctgtcgga agcggcgctg gtcgaccttg gcgtggcgat gggcccggtg
     5101 atcgcctcga tccacgagaa acgggcccag ctccaggccg agatcaagga ggaggccgag
     5161 ctggaggaag agccgcggct cgggcggcgg agactgaggg atgcgggacc tcagacctcg
     5221 ctgtagaagc ttctacctgg tgccacccat agcaccccct ccgcgcccgg atcggggtat
     5281 tcgagctcgc cttcttcgac ctcgagggac tcaggaattg catcccattc gaagcgcttg
     5341 atcagagcaa gtgcgcgtgc tttgtttggg gcagcaacgt cgtaggtcgc gaagaagccc
     5401 ttgtttgcga acggtccggt ctcgtggatc ttcccatgga taatgagtct gccccggcga
     5461 attgcaggta agccacacag gccattcagg taaccaattg cacgaatagc tccggggttc
     5521 gcccgacgca tcacgattgc atcgtcgatg agcgcttcag ctttctcgct aaagccccag
     5581 cgagcgagcc agtatgttgc ccatccgagt agccacccga cgatcgaaat cattcgaata
     5641 ataagtggct cttgatagac gtaaccggcc acttcttccg aattttcctc gatgctctgc
     5701 ggcgatttcg tcatcacgcc gccacgtttt ccagggcgcc gcggaccgcg ctcagcgctt
//
LOCUS       DASTZO010000080         5122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1076364,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000080 DASTZO010000000
VERSION     DASTZO010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076364"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VFU20_02140"
     CDS             complement(<1..279)
                     /locus_tag="VFU20_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285293.1"
                     /translation="MRAGSRFIAALAAALAFAAVPALAQDSSAPATNAPAETIGPSEL
                     QDFSLNGTVTRPAPAPPARTAPAPRPTVQRTAPAPETRAAPAPDRPRAS"
     gene            complement(374..1066)
                     /locus_tag="VFU20_02145"
     CDS             complement(374..1066)
                     /locus_tag="VFU20_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU5285294.1"
                     /translation="MIDIRPFASLGHADHGWLDARHHFSFAGYYDPKRMGWGRIRVWN
                     DDRIAARSGFPPHPHRDMEIVTYVRSGAITHQDSMGNTGRTAAGDVQVMSAGTGVTHA
                     EYNLEDEETTLFQIWIETDRPGAEPGWGAKPFPKDARDGRFQLLASGAAEDGALAINA
                     DARVLGATVRAGESVEIDASPERHLYLVPSGRVRVNGVPARPRDGIAIIGEDRLRIEA
                     DDDAELVLVDAR"
     gene            1260..3437
                     /locus_tag="VFU20_02150"
     CDS             1260..3437
                     /locus_tag="VFU20_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEU5285295.1"
                     /translation="MPKLARLQDWERARGAEPDSRTVGVRPLRYCAFLSYSHADEAVA
                     GWLHDALEKFRTPSSLAGRMTHNGAIPKRLTPIFRDRHELPASDNLAAGIRDALDSSR
                     FLIVLCSPAAAASRWVNAEIDMFKRHRPDGCVLAAIVAGEPFASDVPGREAEECLPPA
                     LRQKYDRRGRPTSKRAEPLAADLREDGDGKRLGLLKLVSGMLGVGLDDLVQRETQRRQ
                     RRLAIVAAASLAGMAVTSGLAVFAFDSRDEARDQRREAEGLVGFMLGDLREKLEPLGR
                     LDTLDAVGSRALGYFEKQDKSELSDEALAQRARALTLIGEIAFTRGNLEGAERRYREA
                     FASTGEAARRYPDDPQRLYDHAQNVFWVGYLAWQRGRPDEAAARFREYRRLAERMVAL
                     APENNDYRREVISAITNLGTVLMDQRRFREAAVTYQASARAAETLAAAEPRNLEYQKQ
                     VIKALAWLADAREYAGELEQALATRERQLQQIAELQKTDPRDMQLHRDAMTTHRSIGR
                     LLATRGDLSGGIKESLAGVALSDTLFRVEPDNTEWLQANVSGRFDLADLQLAAGQIAE
                     AASTIRSACDIVDRLAQRNASVTDWNTTKRVVCLTSRARLALRQGAPAEALWLARQAL
                     PAARRSPKPIDRGLLAMRSLSVGSNALQALGRRDQAVALARQALQSMPPSIELRPSDK
                     AEVATLQLRIGNRAAAQQLISSLAAIGYRHPQYLAAIKEEERT"
     gene            3434..3946
                     /locus_tag="VFU20_02155"
     CDS             3434..3946
                     /locus_tag="VFU20_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285296.1"
                     /translation="MSDTKEGDAKEGDAKEVEPGCTPAVSKPAMGSPKQQPEITQHTI
                     KVKAAANGNSVDWELNGKKPDESKLKLDRDSGGHEIKFDLDPDHVLKGRGLRFNCGYP
                     IFVHDNVATCPSSGIDSQIEVLECSRGSLTIFDKNSGPSKMLRYQLNFVEDDGAAPVC
                     DPIIENGGST"
     gene            4015..>5122
                     /locus_tag="VFU20_02160"
     CDS             4015..>5122
                     /locus_tag="VFU20_02160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285297.1"
                     /translation="MAAPNGTRPPRVPFTLCVGVTGHRQDALPPEFLPRLPGFIRDAL
                     RQLTECAATVRAGAEDCFAADPTRLLFVSPLAHGADQIAAGVALELGFSLHAVLPFTL
                     NDYRRDLINGGAESRFDDLVGKAASVLELPGTRDRAMEAYVMAGRATVAHCDVLVAVW
                     DGLEARGRGGTAEVVQLAIAQGTPVIHLPIDGKNSPRLLWAAFDPLVVTQRPDEMAQR
                     PFDKAHVELMLTAYLLPPVDPQERDFLRRFATERLRRIRARIEYPLMLAAAGVARFKP
                     SQIRESNCMAQTAEEWRRYREAWLNRHSDTASLDLLESAYSWSDQLAGYFAQTYRSGH
                     VFNFVLGGFAVCLGLSAFMAPGASFELALVEFVIA"
BASE COUNT          861 a         1727 c         1707 g          827 t
ORIGIN      
        1 tgacgccctc ggccggtccg gcgccggcgc agcgcgggtt tccggtgccg gagccgtccg
       61 ctgcacagtc ggccgcggtg ccggcgccgt gcgtgcaggc ggggccggtg ccgggcgagt
      121 cacggtgccg ttgagcgaaa agtcctgcag ctcgctcggc ccgatggttt cggcgggcgc
      181 attggtggcc ggcgcgctgc tgtcctgcgc gagcgccggc acggcggcga acgcgagcgc
      241 agccgccaat gccgcaatga agcgcgagcc tgccctcatg tcgtcccgtt cgttgcgagt
      301 gagtcgcaca gccttccggc gcgcgccgcc aactcatggc gcgcaatcgg cgcgtcttca
      361 agcatggcgt ggattaacgg gcgtcgacca gcaccagctc ggcatcgtcg tcggcctcga
      421 tccgcagcct atcctcgccg atgatcgcaa tgccgtcgcg cggccgggcg ggcactccgt
      481 tgacccgcac cctgccgctc gggaccaggt acaggtgccg ctcgggcgac gcgtcgatct
      541 cgaccgactc gccggccctg acggtcgccc cgaggacccg ggcgtcggcg ttgatggcca
      601 gcgccccgtc ctcggccgcg ccgctcgcga gcagctggaa gcggccgtcg cgggcgtcct
      661 ttgggaacgg cttcgcgccc caccccggct cggctcccgg gcggtcggtc tcgatccaga
      721 tctggaacag ggtcgtctcc tcgtcctcga ggttatactc ggcgtgggtg acgccggtgc
      781 cggcgctcat cacctggacg tcgccggccg ccgtgcggcc cgtgttgccc attgaatcct
      841 ggtgggtgat cgcgccgctg cggacgtagg tgacgatctc catgtcgcgg tggggatgcg
      901 gggggaagcc ggatctggcg gcgatccggt cgtcgttcca gacccgaatc cggccccagc
      961 ccatgcgctt cgggtcgtaa taaccggcga acgagaaatg gtggcgggcg tcgagccagc
     1021 catggtcggc gtggccgagc gaggcgaacg ggcggatgtc gatcatggcg tcaataatgg
     1081 cgagcgcccg cgccgcttca agtgacagcg atgtaaccgc cgctccgctc ccgtgacgaa
     1141 ccgagggcag gcgcgctcgc gaagtgcaag ttattgaaaa taggcgggcc gaagcctagc
     1201 gaggttggtg cccgcggtta aattctgata aaccgcggtg cacgcgtggg ggtggcaaga
     1261 tgccgaagct tgcccgactg caggattggg aacgggctcg aggggccgag cccgactcgc
     1321 gcaccgtcgg cgtccggccg ctcagatatt gcgcgttcct gagctacagc cacgccgacg
     1381 aagcggtcgc gggctggctc cacgacgctc tcgagaagtt tcgaacgccc tcgtccctgg
     1441 ccggcaggat gacccacaac ggcgccattc ccaaacgcct gacgccgatc ttccgcgacc
     1501 ggcacgagct gcccgcgtcc gacaacctcg ccgccggaat ccgcgacgcg ctcgactcgt
     1561 cgcggttcct gatcgtgctg tgctcgcccg ccgcggcggc atcgcgctgg gtcaatgccg
     1621 agatcgacat gttcaagcgg caccgcccgg acggctgcgt gctggcggcg atcgtcgccg
     1681 gcgagccgtt cgcgagcgac gttccaggac gcgaggcgga ggaatgcctg ccgccggcgc
     1741 tgcgccagaa atatgaccgc cgcggacggc cgacgagcaa gcgcgccgag ccgctcgcgg
     1801 ccgaccttcg cgaggacggc gacggcaagc ggctcggact gctcaagctg gtctcgggaa
     1861 tgctcggagt cgggctcgac gatctcgtcc agcgcgaaac ccagcgccgc cagcgccgcc
     1921 tggcgatcgt cgccgcagcc tcgctcgccg ggatggcggt caccagcggc ctcgccgtgt
     1981 tcgccttcga ctcccgcgac gaggcccgcg accagcggcg cgaggccgaa gggctggtcg
     2041 gcttcatgct cggcgacctc agggagaagc tcgagccatt gggccgcctc gacacgctgg
     2101 acgccgtcgg cagccgagcg ctcggatact ttgagaagca ggataaatcc gagctatccg
     2161 acgaggccct tgcgcaacgc gcacgcgcac tcacgctcat cggggaaatc gcctttacca
     2221 gagggaatct cgaaggtgcc gaaagacgct accgtgaggc ttttgcaagc acgggcgaag
     2281 ctgcccgccg gtaccccgac gatccgcagc ggctgtacga ccatgcgcag aatgtcttct
     2341 gggtaggtta tctcgcctgg cagcgtggcc ggcctgacga agccgcagcc cgctttcgcg
     2401 aataccggcg cctggccgag cggatggtcg cgctcgcgcc tgagaacaat gactatcggc
     2461 gcgaagtaat ctccgccatc acaaaccttg gcaccgtgtt gatggatcaa cgccgctttc
     2521 gtgaggcggc cgttacatac caggcgtcgg cgcgcgctgc ggagacgctg gcggcagcgg
     2581 agccgcgcaa cctcgaatat caaaagcagg tgatcaaggc gcttgcctgg ttggctgatg
     2641 ctcgcgaata tgccggcgag ctcgagcagg cgctcgccac gcgcgaacgg cagctacaac
     2701 agattgccga gcttcaaaag accgacccgc gcgacatgca gcttcatcgg gacgcgatga
     2761 ccactcaccg gtcgatcggg cgactgcttg ccacgcgcgg agatctctcg ggcgggatca
     2821 aggaatcgct cgcgggggtt gccctttccg ataccttatt ccgggtcgag ccggataaca
     2881 ccgaatggct gcaggcaaat gtcagtggca ggttcgatct tgccgatctg cagctggcag
     2941 caggtcaaat cgccgaagcg gccagcacca tccgatccgc ctgcgacatt gtcgatcggc
     3001 tcgcccagcg caacgccagc gtgacggact ggaacacgac caagcgcgtt gtctgcctca
     3061 cctcgcgcgc gcgcctggcc ctgcgccagg gtgcgccggc cgaagcgctg tggctcgcgc
     3121 gccaggcgct cccggcggcc cggcgctccc ccaagccgat cgaccgaggc ttgctggcca
     3181 tgcgctcatt gtcggtcggg agcaacgcgc tccaagccct gggacgtcgc gaccaggccg
     3241 tggcgttggc gcgacaggcg ctccagtcga tgccgccgtc gattgagctc aggccgagcg
     3301 acaaggccga agttgcaact cttcaactgc gcattggcaa tcgggccgcc gcgcaacaac
     3361 tgatttcatc actcgccgcc atcgggtacc gtcacccgca atatcttgcg gcgatcaaag
     3421 aggaggaacg aacatgagcg acacgaaaga aggcgatgcc aaagaaggcg atgccaaaga
     3481 agtcgagccg ggatgcaccc cggcggtatc caagccagcg atgggcagcc cgaagcagca
     3541 acctgagatc actcagcaca cgatcaaagt gaaggctgcg gcgaacggca attcggtcga
     3601 ctgggagctc aacgggaaga aacccgatga gtccaagctg aagctcgacc gtgactccgg
     3661 cggccacgaa atcaagttcg acctcgatcc ggatcacgtc ctcaagggcc gcggtctgcg
     3721 ctttaactgc ggatatccga tattcgtcca tgacaatgtc gcaacatgtc catcaagcgg
     3781 catcgacagt cagatcgaag tacttgagtg cagccgcggc agcctgacga tcttcgacaa
     3841 gaacagcggt ccttcaaaaa tgcttcgcta tcagctcaat tttgtcgaag acgatggcgc
     3901 cgcaccggtc tgcgatccga tcatcgagaa tggcgggtca acatagtgtg tcggctcacg
     3961 gcctcaggca cggaatgagc tgcggagacc gccatatcag cgcttgagct gcggttggct
     4021 gcgccgaacg gcacgcgccc gccgcgcgtg ccgttcacgc tctgcgtcgg ggttaccggg
     4081 caccgccagg atgcgctgcc gcccgagttc ctgccgcggc tgccgggctt cattcgcgac
     4141 gcgctccgcc agctgacgga gtgcgcggcc acggttcgag ccggcgccga ggactgtttc
     4201 gcggcggacc cgacgcgcct gctgttcgtc tcgccgctcg cccacggcgc cgaccagatc
     4261 gccgccggag tcgcgctcga gctcggtttc tcgctccacg cggtgcttcc gttcaccctc
     4321 aacgactatc gccgcgacct gatcaacggc ggcgccgaat cgcggttcga cgacctggtc
     4381 ggcaaggccg cctccgtcct cgagctgccg ggaacccggg atcgcgcgat ggaggcctat
     4441 gtcatggccg gacgggcgac ggtcgcccat tgcgatgtcc tggtcgccgt gtgggacgga
     4501 ctggaggcgc gcggccgcgg cggcaccgcc gaggtcgttc agctggcgat tgcccaaggc
     4561 actccggtca ttcacttgcc gatcgacggc aagaattcgc cgcggctgct gtgggcggcg
     4621 ttcgatccgt tggtcgtgac gcagcgcccc gacgaaatgg cccagcggcc gttcgacaaa
     4681 gcccatgtcg agctgatgct cacggcctat ctgctcccgc ccgtcgatcc ccaggagcgc
     4741 gatttcctac gccgcttcgc gaccgagcgg ttgcgccgga ttcgggcgcg aatcgaatat
     4801 ccgctgatgt tggcggcggc cggggtcgcg cgcttcaagc cgtcgcagat ccgcgagtcc
     4861 aactgcatgg cgcaaaccgc cgaggagtgg cgccgctatc gcgaagcctg gctcaaccgc
     4921 cattcggaca ctgcctcgct cgaccttcta gaaagcgcct acagctggag cgaccagctg
     4981 gccggctatt tcgcccagac ctatcgcagc ggccatgtgt tcaacttcgt gctcggcggt
     5041 tttgccgttt gcctcggttt gagcgcgttc atggcgccgg gggcgagctt cgagctggcg
     5101 ctggtggaat tcgtgatcgc cc
//
LOCUS       DASTZO010000081         4826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1024523,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000081 DASTZO010000000
VERSION     DASTZO010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4826
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1024523"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="VFU20_02165"
     CDS             complement(<1..550)
                     /locus_tag="VFU20_02165"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HEU5285298.1"
                     /translation="MTVTALIVAAGSGQRLGGGVPKQYRLLGGKPVLRRAVEAFAGHP
                     LVDAVRVVIGRGQDELARAALAGLDVGQPVHGGEQRADSVRAGLAASESGQVLVHDAA
                     RPFCPPHVIDRLIAALASHDGAAPVLAVGDTLARAGELLGEPIDRSGAVRVQTPQAFE
                     LGALRSAYERWTGEPPTDETSVA"
     gene            603..1616
                     /gene="dusB"
                     /locus_tag="VFU20_02170"
     CDS             603..1616
                     /gene="dusB"
                     /locus_tag="VFU20_02170"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU5285299.1"
                     /translation="MSLAKPISVGPISVGPLRIDAPVILAPMTGVTDLPFRRVVKRYG
                     AGLTVSEMIASQAMIRETRQSLQKSLWDPSEEPVSLQLAGCEPEVMAEAARLNEQRGA
                     AIIDINMGCPVKKVVNGDAGSALMRDLPLAAAIIAATVKAVTVPVTLKMRTGWDDATR
                     NAPELARIAEDLGVKMITVHGRTRCQLYKGHADWAFVARVKQAVSVPVIVNGDICSVD
                     DAREALEQSGADGVMVGRGAYGRPWLLGQMIPALRDGGSRPDPSLDEQLATMLEQYDD
                     MLSLYGRQTGVNLARKHIGWYTKGLPGSAELRNRVNSQDDPAVVAAMLRQFYAPWLAR
                     SAA"
     gene            1686..3968
                     /locus_tag="VFU20_02175"
     CDS             1686..3968
                     /locus_tag="VFU20_02175"
                     /inference="COORDINATES: protein motif:HMM:NF039586.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5285300.1"
                     /translation="MDARAAESALTSKANWLARERASGRFYEYAALGIGAALVVIAAF
                     SGWVLSRGAEPGTLLSPPLIALLLVANLIPAIALMVLYSRKVAMARAARGGLGSGRLH
                     TRLVALFSVIAAVPTVLVAIFASLLFQSGLEFWFSDRARGMLENTVQVARSTFDAEVE
                     RVTRETVTMSGDLAGYLRVIAIDDPRFAEAFGRNQVLARNLSEAIIFTYGADRQIRAL
                     ALVNPYDRPLEQVITPDKIAELRSRESVAINSSDRIGALTRLDYGPDTYLYAARVFEP
                     RFQEQISRANEVLRDYRALLSRSRTNQLRFNAALLFGALIIVGLAIFTALKLADRMVR
                     PVGQLVAAAGRIEEGDFTARVPVANTEDEIQTLATAFNRMTGRLDEQTGALKAANAQL
                     ETRRAFIEAVLSSVTAGVIAVDSDNRILLINRSAEALLPRGRRKLEGTDLSRLSPDLD
                     EFMHGDQPEADVQVEVGAGQRTLAVKRVRYQDGAVLTFDDITDQLADQRRAAWSDIAR
                     RIAHEIKNPLTPIQLAAERLQRRFGKDIQSDQETFERLTGTIVRQVGDLRRMVDEFSN
                     FARMPKPAFREENIHEIARQALFLHEVAHPGITFTIDPPDGTLPMVCDRRQLAQALTN
                     VVKNAVEAIETRRNRGEHSLAGDRVELRLKSEGEQLVIDVLDTGIGLPEDRERLTEPY
                     MTTRVRGTGLGLAIVKKIVEEHRGEIAFLDRPGGGTHVRIAFDSERLAKLASEAAPEG
                     GESGGGGDPGDDDDEAEEID"
     gene            3968..>4826
                     /locus_tag="VFU20_02180"
     CDS             3968..>4826
                     /locus_tag="VFU20_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014500384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5285301.1"
                     /translation="MALEVLVVDDEADIRELVSGVLEDEGYAVRAAADSTAALDAIDE
                     RRPSMVLLDVWLQGSRLDGLQLLQEIKRRDASLPVLLISGHGNLDTAVAAVREGAIDF
                     IEKPFEAERLVYLVDRATETDRLKRENVALRRQIGQDEQLNGSSVAINTVRATLKRVA
                     PTGSRVLITGPGGVGKEIAARMIHQWSPRAKAPFIVLSAAMMSPDRVEEELFGSEVDG
                     EARPGLLEQAHGGTLFLDEIADMPHTTQGKILRVLTDQSYHRVGGQRPVKVDVRVLSA
                     TSRNLSEEIA"
BASE COUNT          744 a         1710 c         1690 g          682 t
ORIGIN      
        1 gggcgacgct ggtctcgtcg gtcggcggct cgccggtcca gcgctcgtag gcgctgcgca
       61 gggcgcccag ctcgaacgcc tgcggggtct ggacgcgcac cgcgcccgag cggtcgatcg
      121 gctcgccgag cagctccccg gcgcgggcca gcgtatcgcc gacggccagc accggcgcgg
      181 cgccgtcgtg gctggcgagc gcggcgatca gccggtcgat tacgtgcggc gggcagaaag
      241 gccgcgccgc gtcgtggacc agaacctggc cgctttcgct cgcggcaagg ccggcgcgga
      301 ccgaatccgc gcgctgctcg ccgccatgga ccggctgccc gacgtcgagg ccggcgagcg
      361 ccgctcgggc cagctcgtcc tggccgcggc cgatcacgac ccgcaccgcg tcgaccagcg
      421 ggtggccggc aaaggcctcc accgcccggc gcagcaccgg cttgccgccg agcaagcggt
      481 actgcttggg aacgccgccg ccgaggcgct ggccgctgcc ggcggcgacg atcagtgcgg
      541 ttacggtcat cgggcccgcg ccttgccccc gtatggccgc tcctgtaaag gcgcgccttc
      601 ccatgagcct cgccaagccc atctccgtcg gcccaatctc ggtcggcccg ctccggatcg
      661 acgcgccggt catcctggcg ccgatgacgg gcgtcaccga cctgccgttc cggcgcgtcg
      721 tcaagcgcta cggcgccggg ctcacggtca gcgagatgat cgccagccag gcgatgatcc
      781 gcgagacccg ccagtcgctt cagaaatcgc tgtgggaccc gtcggaggag ccggtgtcgc
      841 tgcagctcgc cggctgcgag cccgaggtga tggccgaggc ggcccgactc aacgagcagc
      901 gcggggcggc gatcatcgac atcaacatgg gctgcccggt caagaaggtg gtcaacggcg
      961 acgccggctc ggcgctgatg cgcgacctgc cgctggccgc ggcgatcatc gccgcgacgg
     1021 tcaaggcggt gacggtgccg gtgaccctga agatgcgcac cggctgggac gatgcgaccc
     1081 gcaacgcgcc cgagctggcg cggatcgccg aggacctcgg cgtgaagatg atcaccgtcc
     1141 acggccgcac ccgctgccag ctgtacaagg ggcacgccga ctgggccttc gtcgcccggg
     1201 tcaagcaggc ggtgtcggtg ccggtgatcg tcaacggcga catctgctcg gtcgacgacg
     1261 cccgcgaggc gctggagcaa tcgggcgccg acggcgtgat ggtcggccgc ggcgcctatg
     1321 gccggccgtg gctgctgggc cagatgatcc cggcgctgcg cgacggcggc tcgcggcccg
     1381 acccgtcgct cgacgagcag ctcgccacca tgctcgagca atatgacgac atgctgtcgc
     1441 tgtacgggcg gcagaccggg gtcaacctcg cccgcaagca tatcggctgg tacaccaagg
     1501 ggctgcccgg ctcggccgag ctgcgcaacc gggtcaacag ccaggacgat ccggccgtgg
     1561 tcgccgcgat gctgcgccaa ttctatgcgc cctggctcgc ccgctcggcc gcctgagcga
     1621 taaaaaggcg tcaatttcgc gcgactgtgt ttttcatgca acgcgaccgt gctagagcca
     1681 cagtgatgga cgcgcgggcc gccgaatcgg cgctcacttc gaaggcgaac tggctggcac
     1741 gggagcgggc gagcgggcgc ttctacgaat atgcggcgct tggtatcggc gccgcgctgg
     1801 tcgtcatcgc cgcgttcagc ggctgggtgc tgagccgcgg cgccgagccc ggcaccctgc
     1861 tgtcgccgcc gctgatcgcg cttctgctgg tcgccaacct catcccggcg atcgctttga
     1921 tggtgctcta ttctcgcaag gtggcgatgg cccgggcggc gaggggaggg ctcggcagcg
     1981 gccggctgca cacccgcctc gtcgctctgt tctcggtgat cgccgcggtt cccaccgtgc
     2041 tggtcgcgat cttcgcctcg ctgctcttcc agagcggcct ggaattctgg ttctccgacc
     2101 gcgcccgcgg aatgctcgag aacaccgtcc aggtcgcccg ctcgaccttc gacgcggaag
     2161 tcgagcgggt gactcgcgag acggtcacca tgagcggcga cctcgccggc tatttgcggg
     2221 tcatcgcgat cgacgacccg cgcttcgccg aggcgttcgg gcgcaaccag gtgctcgccc
     2281 gcaacctgtc ggaagcgatc atcttcacct acggcgccga ccggcagatt cgcgccctgg
     2341 cgctggtcaa tccctacgac cggccgctcg agcaggtgat cactccggac aagatcgccg
     2401 agctccggag cagggaaagc gtcgcgatca actcctcgga ccggatcggc gcgctgaccc
     2461 gcctcgacta cgggcccgac acctacctct acgcggcgcg ggtgttcgag ccgcggttcc
     2521 aagagcagat cagccgcgcc aacgaggtgc ttcgcgacta tcgcgccttg ctgtcgcgat
     2581 cgcgcaccaa ccagctgcgg ttcaacgccg ccttgctgtt cggggcgctg atcatcgtcg
     2641 gcctggccat attcaccgcc ctcaagctcg ccgaccggat ggtgcgcccg gtcggccagc
     2701 tggtcgccgc cgccggccgg atcgaggaag gcgacttcac cgcccgtgtc ccggtggcca
     2761 acaccgagga cgagatccag accctggcga cggcgttcaa ccggatgacc ggccggctcg
     2821 acgagcagac cggcgcgctg aaggcggcca acgcccagct cgagacccgg cgggcgttca
     2881 tcgaggcggt gctgtcgagc gtcaccgcgg gggtcatcgc ggtcgattcc gacaaccgca
     2941 tcctgctgat caaccgctcg gccgaggcgc tcctgccgcg cggccgccgc aagctcgagg
     3001 gcaccgacct gtcgaggctc tcccccgacc tcgacgagtt catgcacggc gaccagccgg
     3061 aagccgacgt ccaggtggag gtcggcgccg gccagcggac gctcgccgtc aagcgggtgc
     3121 gctaccagga cggcgcggtg ctgaccttcg acgacatcac cgaccagctc gccgaccagc
     3181 gccgggccgc ctggtcggac atcgcgcgcc ggatcgccca cgagatcaag aacccgctga
     3241 ccccaatcca gctcgccgcc gagcggctgc agcggcgctt cggcaaggac atccagtccg
     3301 accaggagac gttcgagcgg ttgaccggga ccatcgtccg ccaggtcggc gacttgcgcc
     3361 ggatggtgga cgaattctcc aacttcgcgc gcatgcccaa gcccgcgttc cgcgaggaga
     3421 acattcacga gatcgcccgc caggccttgt tcctgcacga ggtcgcccat cccgggatca
     3481 ccttcaccat cgacccgccc gacggcacgt tgccgatggt ctgcgaccgc cgccagctcg
     3541 cgcaggcgct cacgaacgtc gtcaagaacg cggtggaagc cattgagacc aggcgcaatc
     3601 gcggcgagca cagcctcgcc ggcgaccgcg tcgagctgcg cctcaagagc gagggcgagc
     3661 agttggtcat cgacgtgctc gacaccggca tcgggcttcc cgaggaccgc gagcggctga
     3721 ccgagcccta catgacgacg cgggtccgcg gcaccggcct ggggctcgcg atcgtcaaga
     3781 agatcgtcga ggagcatcgc ggcgagatcg ccttcctcga ccgcccgggg ggagggaccc
     3841 acgtccggat cgccttcgat tccgagcggc tggcgaagct cgcctcggag gccgcgccgg
     3901 aaggcggcga gagcggcggg ggcggcgatc ccggggatga cgacgacgaa gcggaggaaa
     3961 tcgactgatg gcgcttgaag ttctggtggt cgacgacgag gcggacatcc gcgagctggt
     4021 cagcggggtg ctcgaggacg agggctatgc ggtccgggcc gcggccgaca gcaccgcggc
     4081 attggacgcg atcgacgagc gccggccctc gatggtgctg ctcgacgtct ggctgcaggg
     4141 ctcgcggctc gacggcctgc aattgctcca ggagatcaag cgccgcgacg cgagcctgcc
     4201 ggtgctgctg atttccggcc acggcaacct cgacaccgcg gtcgccgcgg tccgcgaggg
     4261 ggcgatcgac ttcatcgaga agccgttcga ggccgagcgg ctggtctatc tcgtcgaccg
     4321 ggcgaccgag accgaccggc tgaagcgcga gaatgtcgcg ctgcgcaggc agatcggcca
     4381 ggacgagcag ttgaacggca gctcggtggc gatcaacacc gtccgggcca ctttgaagcg
     4441 cgtcgcgccg accggcagcc gggttctgat caccgggccc ggcggggtcg gcaaggagat
     4501 cgcggcgcgg atgatccacc agtggagccc gcgcgccaag gcgccgttca tcgtcctttc
     4561 cgccgcgatg atgagccccg accgcgtcga ggaggaattg ttcggctccg aggtcgatgg
     4621 cgaggctcgg ccggggctgc tcgagcaggc gcacggcggc accctgttcc tcgacgagat
     4681 cgccgacatg ccgcacacca cccagggcaa gatcctgcgc gtgctgaccg accagagcta
     4741 tcaccgggtc ggcgggcagc ggccggtcaa ggtcgacgtc cgggtgctgt cggcgacctc
     4801 gcgcaacctg tccgaggaaa tcgccg
//
LOCUS       DASTZO010000082         8263 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_684927,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000082 DASTZO010000000
VERSION     DASTZO010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 8263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8263
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_684927"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VFU20_02185"
     CDS             complement(<1..452)
                     /locus_tag="VFU20_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HEU5285302.1"
                     /translation="MTDRPSDRPHTPLLDTIASPADIRALAKSQLPQLADELRQEVIS
                     AVSVTGGHLGAGLGVVELTVAIHYVFDTPHDKLIWDVGHQAYPHKILTGRRDRIRTLR
                     AGGGLSGFTRRAESDYDAFGTAHSSTSISAGLGFAVARDLGAPGVGEVV"
     gene            complement(579..1019)
                     /locus_tag="VFU20_02190"
     CDS             complement(579..1019)
                     /locus_tag="VFU20_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEU5285303.1"
                     /translation="MHQGESLKSAARQRLTGQGEQWTEMRAAVFDALAGFERPASAYD
                     IAEAVSKAEGRRVAANSVYRILDLFVGANLARRVESANAYVANAHPDCLHDCIFLICD
                     SCGQTVHIDDDSLSGGVRAAAKTAGFTAPRPVIEVRGTCRECEE"
     gene            complement(1076..1453)
                     /locus_tag="VFU20_02195"
     CDS             complement(1076..1453)
                     /locus_tag="VFU20_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerC domain-containing protein"
                     /protein_id="HEU5285304.1"
                     /translation="MAHTLIYTPPFERLDRMAITLSGLCMVHCLATAVALGLLASAGG
                     LLGAAWIHEVGLSLAMMLGALALGRGVADHGFVMPSAVGGLGLGVMAGALTLPHDGSE
                     AMFTVIGVAVLALGHQLNRIAAN"
     gene            1573..2652
                     /locus_tag="VFU20_02200"
     CDS             1573..2652
                     /locus_tag="VFU20_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HEU5285305.1"
                     /translation="MDSALPTELSPPRRRPRLPPRALSSWLFFVAALVFAMVVVGGIT
                     RLTESGLSITEWRPVSGAVPPLSDSDWARAFELYKGTAEYREINGPAGMDLAAFQRIY
                     FWEWLHRLLGRVIGLAFALPLAWFAVRRAIPAGYGWKLAGLLALGAGQGVLGWYMVLS
                     GLADRTDVSHLRLSAHLLLALAIMAALIWVALDLRNLAARGRDSPARLTLLSAATLAI
                     LFVQILFGAWVAGLDAGQVANTWPLMNGRLYPDGIDWSISAPHTLTHDPFLVHFIHRW
                     WAWVAVIALVVLARRARAAGSRAASVAIHSAFGSQILLGIGTVMTGVTLSFALLHQAV
                     AALLVAAAVWGAHIVGNAGNSRPVQ"
     gene            2649..2963
                     /gene="cutA"
                     /locus_tag="VFU20_02205"
     CDS             2649..2963
                     /gene="cutA"
                     /locus_tag="VFU20_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEU5285306.1"
                     /translation="MTIVSVYAVFADAEEAERIGRQMVEERLTACVNILGPCRSIYRW
                     QGALETAEEVPAILKTTRAQADALVARIAALHSYDVPCVAVWPIDKLLVGYAEWVEGS
                     VG"
     gene            2990..3532
                     /locus_tag="VFU20_02210"
     CDS             2990..3532
                     /locus_tag="VFU20_02210"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5285307.1"
                     /translation="MAASLALPRTRLAWPGTRTFAAALLVLAGSVTMSCTQQPAAGDA
                     SAKDEPRPGDESSASDEETVRAVERQRLKLLVAGDAEAARPLHADDFQLVNPVGRALS
                     RDQYMQALSSGYLDYVAWDPGPIEVRMAGPIAAIRYRSELQVTLQGQPRARLPHWHTD
                     VYRKTGGRWQVVWSQATEIK"
     gene            3677..4156
                     /gene="rplM"
                     /locus_tag="VFU20_02215"
     CDS             3677..4156
                     /gene="rplM"
                     /locus_tag="VFU20_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU5285308.1"
                     /translation="MKALMKTTRSLKTAEVDKKWHLIDAEGLVVGRLATIIANILRGK
                     HKASYTPHIDCGDHVVVINAGKVKFTGRKLEQKTYYKHTGYPGGIKEVTAGKVLEGRF
                     PERVLEKAVERMIPRGPLGRQQMRSLHLYTGTEHPHGGQNPATLDVAGMSRKNKVGA"
     gene            4158..4742
                     /gene="rpsI"
                     /locus_tag="VFU20_02220"
     CDS             4158..4742
                     /gene="rpsI"
                     /locus_tag="VFU20_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU5285309.1"
                     /translation="MAKKKTLSDLGALTSPEPEIVEAPEAAEASAEAGAEAAAPEAAA
                     EDAAPPPPVQEEAPLREQQLDKFGRAYATGRRKDAVARVWIKPGSGRIVINEREQEVY
                     FARPTLRLVINQPFEVADRRGAYDVVATVKGGGLSGQAGAVKHGIAQALTRFEPALRT
                     AVKRAGFLTRDPRVVERKKYGRAKARRSFQFSKR"
     gene            complement(4890..5267)
                     /locus_tag="VFU20_02225"
     CDS             complement(4890..5267)
                     /locus_tag="VFU20_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285310.1"
                     /translation="MTELAYALYLLISIAMTVWVAHTLSTSGEVFLVQCFGQNEQLAK
                     STNHLLVVGFYLVSIGFITLTLSYGSEPATWPQMIRFLSGKVGLAVIVLGGMHFFNMH
                     AIARFGRKVSDWLVEGQAYRPQA"
     gene            complement(5342..5896)
                     /locus_tag="VFU20_02230"
     CDS             complement(5342..5896)
                     /locus_tag="VFU20_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5285311.1"
                     /translation="MVRLTQIPEAEAFILHWGEMGTNWGVNRSVAQIHGLLYLSDRPL
                     HAEEISETLTLARSNVSTALKELQSYGIVRRTHVAGDRRDHFVAETDLWELLLRISAE
                     RKRREVDPTIALLAELKQQIDARSDVPQHIRERISRMHEFISTLTSWYEQVRRLPKST
                     LITLMKLGAKVARFVPGGDRKAPN"
     gene            6004..7173
                     /gene="metC"
                     /locus_tag="VFU20_02235"
     CDS             6004..7173
                     /gene="metC"
                     /locus_tag="VFU20_02235"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004121 - cystathionine beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEU5285312.1"
                     /translation="MTDKPQRPDTKLVHGGRRGEWLQGMVNVPVSRTSTVLFEDVAAM
                     EAAYPPRDGTLSYGRNGTPTHWSLAEALTGLEPGAAGTRLFPSGAAAVSFALASVLEP
                     GDELLMVDSAYLPTRTFCDQALKPQGIATRYYDPRSTAAEIEAMLSDSTRAIFMESPG
                     SLTFEVQDVPGICAVARERGIATLLDNTWATPLFFQALAKGVDLAILACTKYVSGHSD
                     VMLGSVSATEGRWDKLNKTARLYGQSVGADDAYLAARGLRTMGVRLRRHEESAIKIAR
                     WLQDQAPIAQVLHPALKSCPGHDYWKRDFTGSSGLFTIVLKEADEAAAMRFVESLTLF
                     GIGYSWGGFESLAVPARPKRTASSAPAGPIVRLHIGLEDPDDLMADLESALQTIG"
     gene            complement(7230..>8263)
                     /locus_tag="VFU20_02240"
     CDS             complement(7230..>8263)
                     /locus_tag="VFU20_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5285313.1"
                     /translation="SASGFVECFGKDETRNAQFAALNPTIQGPQKRTFKYDKLLPNVG
                     AIYDITDEVSAFASYSKGLSVPGTDNLYNSFFFAPDTEQARPDPEKTDSFDLGGRYRS
                     GKIQAQLSGWFTKYTNRIASAYDPELDRNVFRNLGRVDKYGIDGSIAYEPIRELTLYA
                     FGSRNRSKIKDNIQLAGGTGFDCDTSTAPEATLLRNCAFTKGNYESGAPKYTYGFSAL
                     GRLPFVDLGITAKRTGPRYVYDTNVPTFRGDVDPGAPGGVQEIFGATAPAYWLVHLDS
                     RFKLDRLGIRGTYLQVNVYNLFDKFYVGGFGGGLSQSVNTTTGVYGNPGFVQIGAPRT
                     ISATLNLGL"
BASE COUNT         1347 a         2822 c         2740 g         1354 t
ORIGIN      
        1 accacctcgc cgacgcccgg cgcgccgagg tcgcgcgcca ccgcgaagcc caggcccgcc
       61 gagatcgagg tcgaggaatg cgcggtgccg aaggcgtcgt agtcgctctc cgcccgccgg
      121 gtgaagcccg agagcccgcc cccggcgcgg agcgtccgga tccggtcgcg tcgcccggtc
      181 aggatcttgt gcggataggc ctggtggccg acgtcccaga tcagtttgtc atgcggcgtg
      241 tcgaacacgt agtggatggc gaccgtcagc tcgaccaccc cgagcccggc gcccaaatgc
      301 ccgccggtca ccgacaccgc cgagatcact tcctggcgga gctcgtcggc gagctgcggc
      361 aattgcgatt tcgccagcgc ccttatgtcg gcgggcgagg cgatggtgtc gagcagcggc
      421 gtatgcggac ggtcactggg gcggtcggtc atgggcggcg gcttatccgc aagccgcggc
      481 gatgtcacca acgtggccgc tgttcgcggc ccgaagcgga cgttttcagc gattgctcag
      541 gaattgctca ggaatttcta gtcgccgcag tccccgccct attcctcaca ctcgcgacac
      601 gtgccgcgga cctcgatcac cgggcgcggg gcggtgaagc cggcggtctt ggcggccgcg
      661 cgcactccgc ccgacaggct gtcgtcgtcg atgtggacgg tctggccgca gctatcgcag
      721 atcaggaaga tgcagtcgtg caggcagtcg gggtgggcgt tggcgacata agcgttggcg
      781 ctctcgaccc gccgggccag gttggcgccg acgaacaggt cgaggatccg gtagacgctg
      841 ttggcggcga cgcgccgtcc ttcggctttc gagaccgcct cggcgatgtc gtaggcggat
      901 gccgggcgct cgaagccggc gagcgcgtcg aacaccgcgg cgcgcatctc ggtccactgc
      961 tcgccctgcc cagtcagccg ctggcgcgcc gccgatttca gcgactcgcc ctggtgcatt
     1021 tgatgatcgt gccgcgccac agactgcaga taggcctcgg ccgcccttgc ggcaactagt
     1081 tcgcggcgat tcggttgagc tggtggccga gcgccaggac cgcgactccg atcaccgtga
     1141 acatcgcctc gctgccgtcg tgcggcaggg tcagcgcccc ggccatgacg cccaggccga
     1201 gcccgccgac cgcgctcggc atcacgaagc cgtggtcggc gaccccgcgg ccgagagcga
     1261 gcgctccgag catcatcgcc aggctcagac cgacctcgtg gatccacgcc gctccgagca
     1321 gcccgccggc cgaggccagc aggccgagcg ccaccgcggt cgccaggcaa tgcaccatgc
     1381 acagcccaga caaagtgatc gccatccggt cgagccgctc gaacggcggg gtgtagatga
     1441 gcgtatgggc catcaccacc gacatatatc gcggcgctcg tgaagttaca atatcacatc
     1501 accgaagatt ccgcaccgct tggccgccgc atattgccga agctcgcgcg cccggcgcat
     1561 aggcgcggcc gaatggactc cgcgcttccg accgagctgt cgccgccccg ccgccggccg
     1621 cggctgccgc cgcgcgcgct gtcgagctgg ctgttcttcg tcgccgcgct ggttttcgcg
     1681 atggtcgtcg tcggcggcat cacccggctg accgaaagcg gcctgtcgat caccgaatgg
     1741 cgcccggtca gcggcgccgt cccgccgctg agcgattccg actgggcccg cgcgttcgag
     1801 ctctacaagg gcaccgccga ataccgcgag atcaacggcc cggcgggcat ggacctggcg
     1861 gccttccagc gcatctattt ctgggaatgg ctgcaccgcc tgctgggccg ggtcatcggc
     1921 ctcgccttcg ccctgccgct cgcctggttc gcggtccggc gggcgatccc ggccggctac
     1981 ggctggaagc tcgccggcct gctcgcgctc ggcgccggcc agggcgtgct cggctggtac
     2041 atggtcctgt ccggactcgc cgaccgcacc gacgtcagcc acctgcgcct gtccgcgcat
     2101 ttgctgctcg cgctggcgat catggccgcc ctgatctggg tcgcgctcga cctgcgcaat
     2161 ctcgcggcga ggggccggga cagccccgcg cggctcacct tgctgtccgc cgcgacgctc
     2221 gcgatcctgt tcgtgcagat cctgtttggc gcctgggtcg ccggcctcga cgctggccag
     2281 gtggccaaca cctggccgct catgaacggc cgcctgtatc ccgacggcat cgactggtcg
     2341 atcagcgctc cccacacgct cacccacgac ccgttcctgg tccacttcat ccaccgctgg
     2401 tgggcgtggg tggcggtgat cgcgctggtg gtgctcgcgc gccgggcccg cgccgccggc
     2461 agccgcgccg cctcggtcgc catccactcg gcattcggca gccagatcct gctcggcatc
     2521 ggcacggtca tgaccggagt cacgctcagc ttcgccctgc tccaccaggc ggtcgccgct
     2581 ttgctggtcg ccgccgccgt ctggggcgct cacatcgtcg ggaacgctgg caactcgagg
     2641 ccggtccaat gaccatcgtc tcggtctacg cggtgttcgc cgatgccgag gaggccgagc
     2701 gcatcggccg ccagatggtc gaggagcgcc tcaccgcctg cgtcaatatc cttggcccct
     2761 gccgctcgat ctaccgctgg cagggcgcgc tcgagacggc cgaggaagtc ccggcgatcc
     2821 tcaagacgac gcgagcccag gccgacgcac tcgtcgcccg gatcgccgcc ctgcacagct
     2881 acgacgtccc gtgcgtcgcg gtgtggccga tcgacaaatt gctcgtcggt tatgccgaat
     2941 gggtcgaggg cagcgtgggg tgacccggcg cacgaaatca ggaggatcca tggccgcttc
     3001 ccttgccttg ccgcgcacac gccttgcctg gcccggtacg cgcaccttcg ccgccgcgct
     3061 tctggtgctg gcgggaagcg tcacgatgtc gtgcacgcaa cagcccgccg ccggcgacgc
     3121 ttccgccaag gatgagccca ggccgggcga tgagtcttcc gcgagcgacg aggaaaccgt
     3181 ccgggcggtc gagcgccagc ggctcaagct tctggtcgcc ggcgatgccg aagccgcgcg
     3241 gccgcttcat gccgacgatt tccagctcgt caatccggtg ggtcgggcgt taagccggga
     3301 ccagtatatg caggccctgt cgagcggcta tctcgactat gtggcgtggg atcccgggcc
     3361 gatcgaggtc cgcatggccg ggccgatcgc ggcgatccgc taccgatccg agctccaggt
     3421 gactctgcag ggccagcccc gggcccggct gccgcactgg cacaccgacg tctaccgaaa
     3481 gaccggcggg cgctggcagg tggtctggtc gcaggcaacg gagatcaaat aagcgcccgg
     3541 aactgcaagg taacatgata cccgccaaga aacgcccggt tttgcttgac tttcgcgcct
     3601 tccgccgcga ttagcccgct cgagggcggc gctcgcaggt gccgcttttc ctattctagc
     3661 gagaggcaca accgccatga aggcgctgat gaagaccacc aggtcgctca agacggccga
     3721 ggtcgacaag aaatggcatc tgatcgacgc cgaggggctg gtggtcgggc ggctggcgac
     3781 gatcatcgcc aacatcctgc gcggcaagca caaggccagc tacacgccgc acatcgactg
     3841 cggcgaccat gtcgtcgtga tcaacgccgg caaggtcaag ttcaccggcc gcaagctcga
     3901 gcagaagacc tattacaagc acaccggtta cccgggcggg atcaaggaag tgaccgccgg
     3961 caaggtgctc gagggccgct tccccgagcg cgtgctcgaa aaagcggtcg agcggatgat
     4021 cccgcgcggc ccgctggggc gccagcagat gcgctcgctg cacctctaca ccggcaccga
     4081 gcacccgcac ggcggccaga accccgcaac cctcgacgtc gccggcatga gccgcaagaa
     4141 caaggtgggc gcctaagatg gccaagaaga agaccctgtc cgatttggga gcgctgacca
     4201 gccccgagcc cgaaatcgtc gaagctcccg aggccgccga ggcctcggcc gaggccggcg
     4261 ccgaagccgc cgctcccgaa gcggctgccg aggacgccgc tccgccgccg ccggtgcagg
     4321 aggaagcgcc gcttcgcgag cagcagctcg acaagttcgg ccgggcttat gcgaccggcc
     4381 gccgcaagga cgccgtcgcc cgggtctgga tcaagccggg atcgggccgg atcgtcatca
     4441 acgagcgcga gcaggaggtc tatttcgcgc ggccgacgct gcgcctggtg atcaaccagc
     4501 cgttcgaggt cgccgaccgc cgcggcgcct acgacgtcgt cgcgaccgtc aagggcggcg
     4561 ggctgtcggg ccaggcgggc gcggtcaagc acggcatcgc ccaggctctg acccgcttcg
     4621 agcccgcgct gcgcaccgcc gtcaagcgcg ccggcttcct gacccgcgac ccgcgcgtcg
     4681 tcgagcgcaa gaagtacggc cgggccaaag cgcgcagaag cttccagttc tcgaagcgtt
     4741 agtctcctcc ccggaacggg gagggggacc atccggcgcc gattggatgg tggaggggcc
     4801 ggccgcaacg acgccggcgc atctttcgtt gggtcggaag agagagcggc gcgggtcatt
     4861 gccgcgccgc tctttgcgtc tccggtcttt cacgcctgtg ggcgataggc ctggccctcg
     4921 acgagccaat cggagacctt gcgcccgaag cgggcgatcg cgtgcatgtt gaagaaatgc
     4981 atgccgccga gcacgattac ggccaagccc actttcccac tgaggaagcg gatcatctgc
     5041 ggccatgtcg cgggctcgct tccgtagctc agggtcaacg tgatgaagcc gatgctgacc
     5101 aggtagaagc cgacgacgag cagatggttg gtggatttgg cgagctgttc gttctggcca
     5161 aagcattgga ccaggaaaac ctcaccgctg gtcgacaagg tgtgcgcgac ccatacggtc
     5221 atcgcgatcg agatcaggag atacagcgcg taggcaagct cagtcatcgg ttcctcctct
     5281 tcagtaattt cggaaactta cgaaacaatc gaacgcagtc gtgtggctct tgtgcgtctc
     5341 ctcagttagg ggctttgcgg tctccgcccg ggacgaacct ggcgaccttt gcgccaagct
     5401 tcatcaaggt gatgagcgta gactttggaa gtctccgcac ttgctcatac caactagtga
     5461 gagtgctgat gaattcgtgc atccggctaa tccgctcgcg gatgtgctgc ggtacgtcgg
     5521 accgcgcatc gatttgctgc ttgagttcgg cgagcagggc gatcgtcgga tcgacttcac
     5581 ggcgcttgcg ctccgcgctg atgcgcaaca acagttccca aagatccgtc tccgcgacga
     5641 agtgatcgcg gcggtcgcct gcaacatgcg tccggcggac aatgccgtag gactgtagct
     5701 ccttgagcgc ggtcgacacg ttcgaccggg cgagcgtaag cgtctcgctg atctcctcgg
     5761 catggagcgg gcggtccgac agataaagca gcccatgaat ctgcgcgacc gagcgattaa
     5821 cgccccagtt cgtccccatc tcaccccaat gaaggataaa agcctcggct tctggaatct
     5881 gggttaaacg aaccatgttc tctcttttct gtattttcag aaataaccgg cgccctgctt
     5941 gatgtcaacg gccttctagg aggcgcagtt tccgtttgcg acgccagccg gtagaacgcc
     6001 gccatgaccg acaagcccca gcgccccgac accaaactcg tccacggcgg ccgccgcggc
     6061 gaatggctgc agggcatggt caacgtgccg gtgtcgcgga cctcgacggt gctgttcgag
     6121 gacgtcgcgg cgatggaggc cgcctacccg ccgcgcgacg gcacgctgag ctacggccgc
     6181 aacggcaccc cgacgcactg gtcgctggcc gaggccctga ccgggctcga gccgggcgcg
     6241 gccggcaccc ggctgttccc gtcgggcgcc gcggcggtgt cgttcgccct cgccagcgtg
     6301 ctcgagccgg gcgacgagct gctgatggtc gacagcgcct atctgccgac gcgcacattt
     6361 tgcgaccagg ccctcaaacc ccagggcatc gccacgcgct actacgaccc gcgctcgacc
     6421 gccgccgaga tcgaggccat gctctcggac tcgacccggg cgatcttcat ggaaagcccc
     6481 ggctcgctga ccttcgaggt ccaggacgtg ccggggatct gcgccgtcgc ccgcgagcgc
     6541 ggcatcgcca cgctgctcga caacacctgg gcaacgccgc tgttcttcca ggcgctggcc
     6601 aagggcgtcg acctggcgat cctcgcctgc accaaatatg tgtccggcca ctccgacgtg
     6661 atgctgggct cggtctccgc gaccgagggc cgttgggaca agctcaacaa gacggcccgg
     6721 ctgtacgggc agtcggtcgg cgccgacgac gcctatctcg ccgcccgcgg cctcaggacc
     6781 atgggcgtga ggctgcggcg ccacgaggaa agcgcgatca agatcgcccg ctggctgcag
     6841 gaccaggcgc cgatcgccca ggtgctccac ccggcgctca agagttgccc cggccacgac
     6901 tattggaagc gcgacttcac cggctcgtcg ggcctgttca cgatcgtcct caaggaagcc
     6961 gacgaggccg cggcgatgcg gttcgtcgaa agcctcaccc tgttcgggat cggctacagc
     7021 tggggcggct tcgagagcct cgccgttccg gcccgcccca agcgcaccgc gtcgagcgct
     7081 cccgccgggc cgatcgtccg gctgcacatc ggccttgagg accccgacga cctgatggcc
     7141 gacctcgaga gcgccctgca aacgatcggc tgagacagac cgggcggcgc cccccgcgaa
     7201 gggaggcgcc gcccgtcgtg ccggtttggt tacagcccga ggttgagcgt cgctgagatg
     7261 gtccgcggag cgccgatctg gacgaacccg gggttgccgt agacgccggt ggtcgtgttc
     7321 accgattggc taaggccgcc cccgaaccct ccgacataga atttatcgaa caggttgtag
     7381 acgttgacct ggagataggt gccgcgaatc ccgaggcgat ccagcttgaa ccggctgtcc
     7441 aggtggacga gccaataggc cggggccgtc gcgccgaaaa tctcctgcac gccgccggga
     7501 gctccggggt cgacgtcgcc gcggaaagtc ggcacgttgg tgtcgtagac atatcgcggc
     7561 ccggtccgct tggcggtgat tccgagatcg acgaacggca agcggccgag ggccgagaag
     7621 ccgtaggtgt atttcggcgc gccggactcg taattgccct tggtgaacgc gcagttgcgc
     7681 agcagagtgg cctccggggc ggtggacgtg tcgcagtcga agccggtgcc tccggcaagc
     7741 tggatgttgt ccttgatctt gcttcggttg cgggagccga aggcgtagag cgtcagctcg
     7801 cggatcggct cataggcgat cgagccgtcg atcccatatt tgtcgacccg gccgaggttc
     7861 cggaacacat tgcggtcgag ttccggatca taggccgacg cgatgcggtt ggtgtatttg
     7921 gtgaaccagc ccgacaattg cgcctgaatc ttgccgctcc gatagcgccc gccgagatcg
     7981 aagctgtcgg ttttctccgg gtcgggtctt gcctgctcgg tgtccggggc gaagaagaag
     8041 ctgttgtaca gattgtccgt accggggacc gacagcccct tcgaataact cgcgaaggcg
     8101 ctcacttcgt cggttatgtc atagatcgcg ccgacgttcg gaagcagctt gtcatacttg
     8161 aacgtgcgct tctgcggccc ctggatcgtc gggttgagag ccgcgaactg ggcgttgcgc
     8221 gtctcgtcct tgccgaaaca ttcgacgaag cccgaggcac tcg
//
LOCUS       DASTZO010000083         3253 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1155011,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000083 DASTZO010000000
VERSION     DASTZO010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3253
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155011"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1647)
                     /locus_tag="VFU20_02245"
     CDS             complement(<1..1647)
                     /locus_tag="VFU20_02245"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5285314.1"
                     /translation="MVGPDGRGFAADLGQLYRFLSPARRRDLFSVLLLMLLGALAELV
                     AIGSVVPFLSLLAGDSAQIHFRWLAGLFEAAGAATRGEQLVAASVLFMAAALVAGALR
                     LQLAWSTQTFVLSLGHELAVEIQRRTLAQPYSYHVSRNSSEIVASLEKVRALVFGVLL
                     QLMRAVTAGFIALFIIAALVRIDPFTAAVAAAAFGLLYALVSAFTRRRLARNSAVLGS
                     AYEQRVQIIQESLGGIRDVIIDDSQAVYLEEFRKVDARLTRAQATTAFIGTAPRFVIE
                     AAGMVLIAALALAIAGREGGLAGALPILGAVALGAQRLLPLVQQLYTAWANLAGQRSI
                     TAQVLDLLALPLDEQAKDSRPVERLGFDKSIAFDRVGFTYPGRAHPALEDVSLTIERG
                     SRVALVGPTGSGKSTLADLLMGLLEPGAGTISIDGVPLTRASRRAWQRGIAHVPQSIF
                     LADSSIARNIAFGVPEADIDRERVAAAAAAAQLDAFIESLPGGYDSPVGERGVRLSGG
                     QRQRLGIARAIYKDAPVLVLDEATSALDQDTEAAVMAALDR"
     gene            2256..2780
                     /locus_tag="VFU20_02250"
     CDS             2256..2780
                     /locus_tag="VFU20_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HEU5285315.1"
                     /translation="MKRVVCAAVAALMVYSTAAHSAPRLYSRGADTAERLLAAHNVER
                     VRVRVAPLQWDPQLAASAAAYGPMLASIGRLQHSPRAIRPGQRENLWMGTRGSFSPEQ
                     MVGTWVDERRHFRPGIFPDVSRTGNWADVSHYTQLIWKGTTRVGCAIHSTPRWDFLIC
                     RYSPPGNVDGWWLP"
     gene            2871..>3253
                     /locus_tag="VFU20_02255"
     CDS             2871..>3253
                     /locus_tag="VFU20_02255"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU5285316.1"
                     /translation="MPADLQDDLSSSLSTIGGRWLVTGAAGFIGSNLVEALLTAGRQV
                     VGLDNFATGHRGNLDEVRAAVGDERWSGFQLIEADIRDRDACARAVNDVEIVLHQAAL
                     GSVPRSLADPLTSHDVNVTGFVNML"
BASE COUNT          510 a         1132 c         1141 g          470 t
ORIGIN      
        1 ccggtcgagc gccgccatca ccgccgcctc ggtgtcctgg tcgagcgcgc tggtcgcctc
       61 gtcgagcacc agcaccggcg cgtccttgta gatcgcacgg gcgatcccga gccgctggcg
      121 ctgcccgccg gacagccgga cgccgcgctc gccgaccggg ctgtcgtagc cgccgggaag
      181 cgattcgatg aaggcgtcga gctgcgccgc ggcggccgct gcggcgaccc gctccctatc
      241 gatgtccgct tccgggaccc cgaaggcgat gttgcgggcg atgctggagt cggcgaggaa
      301 gatcgactgc gggacgtggg cgatgccgcg ctgccaggcg cgccggctcg cccgggtcag
      361 cggcaccccg tcgatcgaaa tcgtcccggc gccgggctcg agcaggccca tcagcaggtc
      421 ggcgagcgtg ctcttgccgc tgccggtcgg gccgaccagc gcgacccgcg agccgcgctc
      481 gatggtcagg ctgacgtcct cgagcgccgg atgggcgcgc cccggatagg tgaagcccac
      541 ccggtcgaac gcgatgctct tgtcgaaccc cagccgctcg accggccgcg aatccttcgc
      601 ctgttcgtcg agcggaagcg caagcaggtc gagcacctgg gcggtgatcg agcgctgccc
      661 ggcaaggttc gcccaggcgg tgtagagctg ctggaccagc ggcagcaggc gctgggcgcc
      721 gagcgcgacc gcgccgagga tcggcagcgc cccggcgagc ccgccctcgc ggccggcgat
      781 cgccagcgcg agcgcggcga tgaggaccat cccggcggcc tcgatcacga accgcggcgc
      841 ggtgccgatg aaggccgtgg tcgcctgcgc ccgggtcagg cgggcgtcga ccttgcggaa
      901 ttcctcgagg tagacggcct gagaatcgtc gatgatgacg tcgcggatcc cgccgaggct
      961 ctcctggatg atctgcaccc gctgctcgta agcggagccg agcaccgccg agttgcgggc
     1021 cagccgccgg cgggtgaagg ccgagaccag cgcgtagagc agcccgaacg cggcggccgc
     1081 gaccgccgcg gtgaacgggt cgatgcgcac cagcgctgcg atgatgaaga gtgcgataaa
     1141 gcccgcggtc accgcccgca tcagctgcag gagcaccccg aacacgagcg cccggacctt
     1201 ttcgagtgat gcgacgatct cgctcgaatt gcggctgaca tggtaggaat agggctgcgc
     1261 cagagtgcgc cgctggatct ccactgcgag ctcgtggccg aggctgagga cgaaggtctg
     1321 ggtcgaccag gcgagctgca gccggagcgc tccggcgacc agcgcggcgg ccatgaacag
     1381 gacgctcgcc gcgaccagtt gctcgccgcg cgtggccgct ccggccgctt cgaacaggcc
     1441 ggccagccag cggaaatgga tctgcgcgga atcgccggcg agcagcgaca ggaacggcac
     1501 caccgagccg atcgcgacca gctcggcgag cgcgccgagc agcatcagca gcagcaccga
     1561 aaacaggtcc cgccggcgag ccggcgagag gaaccggtac aattgcccga ggtccgccgc
     1621 gaatccgcgg ccgtccgggc ctaccacgcg gattcctcgg gccgatgcga ttgcgcgctc
     1681 attggcgcgc aatagcagct tgcgcactca ttgtgaggcc gtgcctgggc ggatgcccgc
     1741 ccgggcgttc agtcggaggc cgcgggcatt cctgcaaagc cgcccgatcg gaacactggc
     1801 agctcggaaa ccggcagcca gcggccgggg ccgatccgct gcatccgggt gccgcacaag
     1861 tggcacttgc ttcgccaggt gccgaccgac ggccgcgcgc ggcgccggct acgatgatgc
     1921 cccaacaggc tgcacaggat gttcggcatt gaacccccca ccaaatcgcg cccgggatgg
     1981 gcgcgccttc attgctcgag gttgggcggt ttcggcttcg ctcagtctcc ggccttaggg
     2041 gtaccgggcc gaatgaaatt aatcaatgtt tttaatatga attaaccagt atcgagccaa
     2101 gccactgatt tgaacgcgtt tcgcaaaccg cgtcgtcccg gcgtccggac gcaacgccct
     2161 gccgcaaacc ggcgccgatt cagctgccgt tagcacttcg aacgctaggg atggcgtgtt
     2221 cgtccaagcc ggtggggtgg gagcaagttc aggaaatgaa gcgcgtggtc tgcgccgccg
     2281 tggcggcgct tatggtctat tcgacggcgg cccattcggc gccgcggctc tactcgcgcg
     2341 gtgccgacac ggccgagcgc ctgctcgcgg cgcacaatgt cgagcgcgtg cgggtccggg
     2401 tcgcgccgct gcagtgggac ccgcaactcg cggcctcggc ggcggcctac ggcccgatgc
     2461 tcgcgtcgat cggccgcctc cagcactcgc cgcgcgcgat tcggcccggc cagcgcgaga
     2521 atctgtggat gggaacccgc ggctcctttt cgcccgagca gatggtcggc acctgggtcg
     2581 acgagcggcg ccatttccgt cccggcatct ttcccgacgt cagcaggact ggcaattggg
     2641 ccgacgtctc ccactatacg cagctgatct ggaaggggac gacgcgggtg ggctgcgcga
     2701 tccattcgac gccgcggtgg gattttttga tctgccgcta ctcgccgccg ggcaacgtcg
     2761 acggctggtg gctgccgtaa ccatccggcg attgcattga cgcacttccg gtcgggcgcc
     2821 ggctggcgct aagggggcag ggcttccaac taccagcgag cccagcccga atgccagccg
     2881 acctccagga cgacctttcc agcagcctga gcacgatcgg cggccgctgg ctggtgaccg
     2941 gcgccgccgg tttcatcggc tcgaacctgg tcgaggcgct gctgaccgcc ggccgccagg
     3001 tcgtcgggct cgacaatttc gccaccggcc accgcggcaa cctcgacgag gtgagggcgg
     3061 cggtcggcga cgagcgctgg tcgggattcc agctgatcga ggccgacatc cgcgaccgcg
     3121 acgcctgcgc gcgggcggtc aacgacgtcg agatcgtcct ccaccaggcg gcgctcgggt
     3181 cggtgccgcg ctcgctggcc gacccgctca ccagccacga cgtcaacgtc accggcttcg
     3241 tcaacatgct cga
//
LOCUS       DASTZO010000084         5447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_214457,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000084 DASTZO010000000
VERSION     DASTZO010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_214457"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VFU20_02260"
     CDS             <1..868
                     /locus_tag="VFU20_02260"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5285317.1"
                     /translation="SIAANPRLELAATSSRSGVGPAPQFTDWRRLLDEVEGLEAVAIT
                     TPPSVRYEIARECVERGLHVLLEKPPTVTLTEIEDLACLAEGRGVSLFTTWHAQHNPA
                     VAAAAEALAGKRIAAMDILWHEDVRKWHPGQRWIWEPGGFGVFDPGINAFSIATRIFP
                     GTLFVSGAELHFPEDAQTPIAAEIEFASAVADGPLRCSLDWRRTEGEEWTIRIRTADG
                     LEVRLDNGGSLLSIDGATRAMDSPGEYPDIYARFVELIDERRSHVDVAPLRLVADCLL
                     VGRRHMVEAAPA"
     gene            921..1685
                     /locus_tag="VFU20_02265"
     CDS             921..1685
                     /locus_tag="VFU20_02265"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5285318.1"
                     /translation="MDLAVTDELERTDQAFAANRLLATFPAEARALLEPYSSVVDLDL
                     GSVVHACGQNVVTSYFPFGPTMVSLCLDLSDGRTVEVASIGQEGAVGGIVSCGHAPAF
                     ARAEVIVAGPALRIPMDMLEEAKMRSGHIRNLFCRFSDYLLSQVMQSVACNAFHPIEA
                     RASRWLLTAQDRAGSRIELTQEALAGLLGAQRTTVNAVARILQDEGLISSRRGAIQVL
                     DRVGLRRRSCECYAAIERHFGGIIGTGGIGGDPDCV"
     gene            complement(1716..2216)
                     /locus_tag="VFU20_02270"
     CDS             complement(1716..2216)
                     /locus_tag="VFU20_02270"
                     /inference="COORDINATES: protein motif:HMM:NF017454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HEU5285319.1"
                     /translation="MYLLALIAALAAAGDRDGKTMAVDDWHALSIYGVAGDGSGPTYL
                     QVHAGDLDGDGLPDDAVVRLTCAAGQLQAAHYNVKSPRDSASGMASGKRMHKPITVTK
                     EWDAATPQLMKVKPTYNIKEAKGARMTPDGGGWTSLALANADGLCTASQAAVKATKTR
                     SNIQNN"
     gene            complement(2283..3308)
                     /gene="pyrC"
                     /locus_tag="VFU20_02275"
     CDS             complement(2283..3308)
                     /gene="pyrC"
                     /locus_tag="VFU20_02275"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU5285320.1"
                     /translation="MALQTLTIRRPDDWHVHLRDGTMLNAVAPYTARQFARAIVMPNL
                     DPPVTSVDAARAYRQRIAAAAGDGFTPLMTCYLTDEAEPGEIARGFEAGVWAAAKLYP
                     ANATTNSALGVTDVRNLRPALERMQAIGMPLLVHGEVTDPEVDIFDREAVFIERVLEP
                     LTSDFPALRIVLEHITTAEAADFVRDAGPNVAATVTPHHLAINRNALFAGGLRPHAYC
                     LPVAKRERHRRAIRAAATSGSSKYFLGTDSAPHPRGAKEGPCGCAGIFNAPFALESYA
                     AVFEEEQALDRFEGFASEHGPRFYGLPLNEGTITLERADSAVPDEIAGIVPFQAGEIL
                     RWRLADG"
     gene            3331..4044
                     /locus_tag="VFU20_02280"
     CDS             3331..4044
                     /locus_tag="VFU20_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HEU5285321.1"
                     /translation="MPATTLPPTTLADRAVIRLSGEDVRGFVQGLVTSDVEGPLPVWA
                     GLLTPQGKCLFDFFVWEQGQDLLLDCEAAAADDLAKRLAIYRLRRPISIERDPALAVH
                     WSCEGPEGVPDPRLADLGRRWLAAPSEPALGWLEHRLRLGVTEGRAELGDILWLECNA
                     AELNGVSFSKGCFVGQENTARMNWRSKVNRRLLVVETAQAGPRTRIAYSELGLAVEHR
                     RLDDLSGALVPPWLEPALA"
     gene            complement(4041..4715)
                     /locus_tag="VFU20_02285"
     CDS             complement(4041..4715)
                     /locus_tag="VFU20_02285"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HEU5285322.1"
                     /translation="MANAGNAEIVDLSHLIEHGMATYQGLPGPHICDFWTREGSAANY
                     DDGSSFQIGRIDMVANTGTYLDSPFHRYADGKDLATLPLETLADLPGVVVRRPHGNGI
                     ATDAADLEGVEVRGRAVLIHTGWDEHWRTDAYAVDHSFLTAAAAGWLVEQGAALVGID
                     SYNIDDTRRRSRPVHSMLLAAEIPICEHMTGLGRLPDEGFRFSAVPPKIAGMGTFPVR
                     AFAVIG"
     gene            complement(5130..>5447)
                     /locus_tag="VFU20_02290"
     CDS             complement(5130..>5447)
                     /locus_tag="VFU20_02290"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833108.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HEU5285323.1"
                     /translation="LGVRAVIAESFERIHRSNLVGMGVLPLQFHDGDTADSLGLDGSE
                     TFSIAGVAGIEPRQDVEVQLTDSDGSSRSFSARCRIDTYNELEYFHAGGILHYVLRRL
                     AAA"
BASE COUNT          832 a         1931 c         1825 g          859 t
ORIGIN      
        1 gtcgatcgcc gccaacccgc gcctggaact cgccgcgacc tcaagccgct cgggcgttgg
       61 gcccgcgccg cagttcaccg actggcgacg gttgctcgac gaagtcgagg ggctggaggc
      121 cgtcgccatc accaccccgc cgtcggtgcg ctacgagatc gcccgcgagt gcgtcgagcg
      181 cggcctgcac gtgctgctcg aaaagccgcc gaccgtcacc ttgaccgaga tcgaggatct
      241 cgcctgcctc gccgaggggc gcggggtgtc gctgttcacc acctggcacg cgcagcacaa
      301 tccggcggta gccgccgccg ccgaggcgct cgccggcaag cgcatcgcgg cgatggacat
      361 cctgtggcac gaggatgtgc gcaagtggca cccgggccag cgctggatct gggagccggg
      421 cggcttcggg gtgttcgacc ccggcatcaa cgccttctcg atcgccacca ggattttccc
      481 cggcaccctg ttcgtgagcg gcgccgagct ccattttccc gaggacgcgc agaccccgat
      541 cgcggccgag atcgagttcg ccagcgcggt cgccgacggg ccgctcaggt gcagcctcga
      601 ctggcgccgg accgaagggg aggaatggac gatccgaatc cgcaccgccg acgggctcga
      661 ggtgcggctc gacaacggcg gctcgctcct ctcgatcgac ggcgcgaccc gggcgatgga
      721 cagcccgggc gaatatcccg acatctacgc ccgcttcgtc gagctgatcg acgagcgccg
      781 gagccatgtc gacgtcgctc cgctgcgcct ggtcgccgac tgcctgctgg tcggccgccg
      841 ccacatggtc gaagcggctc ccgcatgata atttggcgac tcggcgagag tcgtgccgct
      901 cgttggggga gggtgaggac atggacctgg cagtcaccga cgagctggag cggaccgacc
      961 aggcgtttgc cgccaaccgg ctgctggcga ccttcccggc cgaggcccgg gcgctgctcg
     1021 agccttattc gtcggtcgtc gacctcgacc tcggctcggt cgtccatgcc tgcgggcaga
     1081 acgtcgtcac cagctacttc ccgttcggac cgacgatggt ctcgctgtgc ctcgacctca
     1141 gcgacgggcg caccgtcgag gtcgcgtcga tcggccagga aggcgcggtc ggcggaatcg
     1201 tcagctgcgg ccacgccccc gctttcgcgc gcgccgaggt gatcgtcgcc gggccggcgc
     1261 tgcggatccc gatggacatg ctcgaggaag cgaagatgcg ctcgggccac atccgcaacc
     1321 tgttctgccg cttttccgat tatctgctca gccaggtgat gcagtcggtc gcctgcaacg
     1381 cgttccaccc gatcgaggct cgcgcgtcgc gctggctgct gaccgcccag gatcgcgccg
     1441 gaagccggat cgagctgacc caggaggcgc tcgccgggct gctcggggcg cagcggacga
     1501 ccgtcaacgc ggtcgcccgg atcctccagg acgagggcct gatcagcagc cgccgggggg
     1561 cgatccaggt cctcgaccgg gtcggcctca ggcggcggtc gtgcgaatgc tacgccgcga
     1621 tcgagcgcca tttcggcggc atcatcggca ccggcggaat cggcggcgac cccgactgcg
     1681 tctagccgcc gccgacggcc gcggccggcc gcgcgtcaat tgttctggat gttcgaccgc
     1741 gtcttggtcg ccttgaccgc cgcttggctc gccgtgcaca ggccgtcggc attcgccagg
     1801 gccagcgacg tccagccgcc gccgtccggc gtcatcctgg cgcccttggc ttccttgatg
     1861 ttgtaggtcg gcttgacctt catcagctgc ggggtcgccg cgtcccattc cttggtgacg
     1921 gtgatcggct tatgcatcct cttgccgctc gccatccccg acgccgaatc gcgcgggctc
     1981 ttcacattgt aatgcgcggc ctggagctgg cccgcggcgc aggtcagccg gacgaccgca
     2041 tcgtcgggca gcccgtcgcc gtcgaggtcg ccggcgtgca cctgcagata ggtcgggccc
     2101 gagccgtcgc cggccacccc gtagatcgac agagcgtgcc agtcgtcgac cgccatggtc
     2161 ttgccgtcac ggtcgccggc cgccgcgagc gcggcaatga gcgccagtag atacatcgca
     2221 aatccctccc tcaaatcgcg gcgagggtgc gccgattcgg ccctgtttgc aacgcagcgg
     2281 aattaaccat ccgcgagccg ccagcgcagg atttcgcctg cctggaacgg cacgattccg
     2341 gcaatctcgt ccggaacggc gctatctgcg cgctcgagcg tgatcgtccc ctcgttgagc
     2401 ggcagcccgt agaagcgcgg cccgtgctcg gatgcgaacc cctcgaagcg gtcgagcgcc
     2461 tgctcctcct cgaacaccgc cgcatagctt tcgagcgcga acggggcgtt gaagatgccc
     2521 gcgcagccgc aaggcccttc cttggctccg cggggatgcg gggcgctgtc ggtgccgagg
     2581 aaatatttgg acgagcccga ggtcgcggcc gcgcggatcg cccggcggtg acgctcgcgc
     2641 ttggcgaccg gcaggcagta ggcgtggggc ctgaggccgc cggcgaacaa ggcgttgcgg
     2701 ttgatcgcca gatgatgggg ggtcaccgtc gccgcgacgt tgggccccgc gtcgcggacg
     2761 aagtccgccg cctcggcggt ggtgatatgc tcgagcacga tcctgagcgc cgggaagtcg
     2821 ctcgtcagcg gctccagcac cctctcgatg aacaccgcct cgcggtcgaa tatgtcgacc
     2881 tcggggtcgg tcacctcgcc gtgcaccagc agcggcattc caatcgcctg catccgctcg
     2941 agcgccgggc gcaggttgcg cacgtcggtt accccgagcg cgctgttggt ggtcgcgttc
     3001 gccgggtaga gcttggccgc ggcccaaacg cccgcctcga agccgcgggc gatctcgccc
     3061 ggctcggcct cgtcggtcag atagcaggtc atcagcgggg tgaagccgtc gccggcggcc
     3121 gcggcgatcc gctgccggta ggcccgcgcc gcgtcgacgc tagttactgg cgggtcgagg
     3181 ttgggcatga cgatcgcccg ggcgaactgc cgcgccgtgt agggcgccac cgcgttcagc
     3241 attgtgccgt cgcgcaaatg gacgtgccag tcgtcggggc ggcggatggt cagcgtttgc
     3301 aatgccacgg cggctcctta actgagggcc atgcctgcga ccaccctgcc cccgacgacc
     3361 ttggccgacc gcgccgtgat ccgcctgtcc ggcgaggacg tccgcggctt cgtccagggc
     3421 ctcgtgacca gcgacgtcga ggggccgctc ccggtctggg cggggctgct caccccgcag
     3481 ggcaagtgcc tgttcgattt cttcgtctgg gagcagggcc aggacctgct gctcgactgc
     3541 gaggccgcgg cggccgacga tctcgccaag cgcctcgcca tctaccgcct ccgtcgtccg
     3601 atcagcatcg agcgcgaccc cgcgctcgcc gtccactggt cgtgcgaagg ccccgaaggc
     3661 gtgcccgacc cgcgccttgc cgacctcggc cgccgctggc tcgcggcgcc gtccgaaccg
     3721 gccctcggct ggctcgaaca ccgccttcgc ctcggggtga ccgaaggccg cgccgaattg
     3781 ggcgacatcc tctggctcga gtgcaacgcc gccgaattga acggggtcag tttctccaag
     3841 ggctgcttcg tcggccaaga gaacaccgcc cggatgaact ggcgctcgaa ggtcaaccgc
     3901 cggctgctgg tcgtcgagac cgcgcaggcc ggaccgcgca cccgaatcgc ctattccgag
     3961 ctcggactcg ccgtcgagca ccgccgcctc gacgacctat ccggagcgct cgtcccgccc
     4021 tggctcgagc cggcgctggc ctagccgatc accgcgaacg cgcgcaccgg gaaggtgccc
     4081 atgccggcga tcttgggcgg caccgccgag aaacggaaac cctcgtcggg cagtcgccca
     4141 aggccggtca tatgctcgca gatcgggatc tcggccgcga gcagcatcga gtgcaccggc
     4201 cgcgaccggc ggcgcgtgtc gtcgatattg tagctgtcga tcccgaccag cgcggcgcct
     4261 tgctcgacca gccagccggc ggccgccgcg gtcaggaacg aatgatcgac ggcataggcg
     4321 tcggtccgcc aatgctcgtc ccagccggtg tggatcagga ccgcccgccc gcgcacctcg
     4381 accccttcga ggtccgccgc atcggtcgcg atcccgttcc cgtgcggccg ccgcaccacc
     4441 actcccggca agtccgccag cgtctccagc ggcagcgtgg cgagatcctt gccgtcggcg
     4501 tagcggtgga acgggctgtc gaggtaggtg ccggtgttgg cgaccatgtc aatccgcccg
     4561 atctggaagc tcgagccgtc gtcgtaattc gccgccgacc cctcgcgggt ccagaaatcg
     4621 cagatgtgcg gccccggcag gccctggtag gtggccatgc cgtgctcgat caggtggctg
     4681 aggtcgacta tctccgcatt gccggcattc gccacagcgc cactccaccg atcatcatcc
     4741 agatcacgta cccacgggca acctcgacga acaggtttat ccgcctctat ctcgcgagag
     4801 cgatttgcgc acgtaagcag caagcgtggt gaccagcatc ccaggcaacg agtacattag
     4861 caaaaatgcg ataccgatta cgaacaaggg accgaagcgt ccggtggtca aaatcatgat
     4921 cgggaaaagg gtcaacatca ccgcaagtgg ccaccgccaa ggccgctgcg gaaacgcaaa
     4981 tcctaggacg ccgcaaagca cataggcagc taagagatag atattccaat ttgcatcgtc
     5041 ccacgcctcc cgcggatacg agcgcaggct ggctccggcc caaagcaccg ctgcggcgca
     5101 aatggacacg agccagccga gtcggttgct cacgccgcgg ccagcctccg aaggacgtag
     5161 tgcaggattc ccccggcgtg gaaatattcg agctcgttgt aggtgtcgat tcggcaccgc
     5221 gccgagaagc tgcggctcga accgtcgctg tccgtcagct ggacctcgac gtcctggcgc
     5281 ggctcgatcc cggcgacccc ggcgatgctg aacgtctcgc tgccgtcgag gccgaggctg
     5341 tcggcggtgt cgccgtcgtg gaactgcagc ggcagaacgc ccatgccgac caggttcgag
     5401 cggtggattc gctcgaagct ctcggcgatc accgctcgga cgcccag
//
LOCUS       DASTZO010000085         9987 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_920182,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000085 DASTZO010000000
VERSION     DASTZO010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 9987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9987
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920182"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..409)
                     /gene="sdhC"
                     /locus_tag="VFU20_02295"
     CDS             complement(2..409)
                     /gene="sdhC"
                     /locus_tag="VFU20_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HEU5285324.1"
                     /translation="MATDQSSPRPRPLSPHLTIWRWGPGMLVSIVHRMTGAALAIAGL
                     ALLTWWLMAIADGADAYADFTAAAAHPLGLVVLVGLTWAFFQHLLSGIRHLVMDSGAG
                     FELDTNKRFAILTLVGSLVLTLLIWVPVLGGLR"
     gene            599..1888
                     /locus_tag="VFU20_02300"
     CDS             599..1888
                     /locus_tag="VFU20_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5285325.1"
                     /translation="MSGDIGHLQRLTNEQVAARIAAYGDDGRFEASLRRLWDEAGSVV
                     EAAARSRFGDDVGCLGRDHFTRPVDEAWVRSVAEQGVRMFAAKRSVPDFIAARAAVAN
                     EVIRAFDECFAGRSDERVEAIGTFHRLLSFSEDIILAQIALLEANEAADQRGRQSEQF
                     ERRVADLVRASTEQSKALTDRTRATAASARGMLGKTSEVAAAAEQSAVAMREAAQTAA
                     GLIRAIEDARSEVETAAGVAIRAGGQAEQAVKVSQALSSHVEAIESILSLIRDIAGQT
                     NLLALNATIEAARAGDAGRGFAVVAQEVKSLASQTARATDDITNKITAIQQATRQTVE
                     ANSSIQGTVEEVQTSADRIREAMELQAQTVTMITAAVDETALAADSMSSTIAAIRSDT
                     ENVAKDIDEVEHGFGRFSEQISDFKSTTKEFVSGIAA"
     gene            1926..2630
                     /locus_tag="VFU20_02305"
     CDS             1926..2630
                     /locus_tag="VFU20_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5285326.1"
                     /translation="MNILVTGASRGIGAAAFALLKSTGHRVAGHSTKGGGELIAGDLA
                     DADAPRALWTAALERLGGRIDVLVNNAGIYEAVADDSADGEWHSAWNRTLTINLQAPA
                     DLSRLAIGHFRERGGGGRIVNIASRAAFRGDSPQHWHYAASKAAMIGMTRTIARGYAA
                     DGILAFAVAPGFTVSEMTDEYLAGRGGAQIVADIPLGRVATTDEVAEAIRWLAADAPA
                     SATGTVIDVNGASYVR"
     gene            2635..3519
                     /locus_tag="VFU20_02310"
     CDS             2635..3519
                     /locus_tag="VFU20_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEU5285327.1"
                     /translation="MSWRATLPCTRVQAESLPDAHELLPLGDSQPVLVADEPDPARPN
                     EWLLHAYFDQEPSAEVLEALARLGCGAPSVEQLGEDDWVAMSQSGLQPIRAGRFFVHT
                     PTHRGAAPSGSIAFEIDASLAFGTGQHATTSGCLEALDSLERNGCTFANIADVGTGTG
                     LLAFAALALWPESRCIGTDIDSVAIDVARDNAAINGVALGHGPGELLLAVADGMDSPL
                     LSARAPFDLIIANILAGPLVELAPSFAEGLADGGTVILAGLLDAQADAVARAYRDLGL
                     ALDKSGTGEWPVLTLVKR"
     gene            complement(3516..5630)
                     /locus_tag="VFU20_02315"
     CDS             complement(3516..5630)
                     /locus_tag="VFU20_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010338800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5285328.1"
                     /translation="MKRSTILLALAAVPALVQAQTAAPPSPPAVEKRPFTVKGPLDRA
                     DEYYWLRDDSRKQPEMLAYLKAENAYADSVLAPLRPLEDKLYSEIVGRIKQDDSSVPV
                     RERGYYYYTRFEVGADYPVVARRRGSMTAPEQVLLDQPAMAKGHGFFAIGDYRVSQDN
                     RLLAYAEDVVGRRQYKLKVKDLATGKALADVVENVEPNLVWADDNRTLYYVEKDPVTL
                     LSKRVKAHVLGTPASADRLIYEEKDDSFYMGVGRTSDDRFICIFLGSTVSNEQRCTSA
                     STPGEFAVLAPRERDFLYTADHVGDRWVIRTNWNAPNYRLMTVRDSAAAAGRSAWSDL
                     VPHDPKVFIEGFQPFSSFIGIEERAGGNKRLRLLANSGRSRLVGSDEPAYTMSLDDNR
                     EVASTRLRYTYDSLTTPKITYEVDSRTGARTVLKRTPSPNYDPADYVTERLWAPARDG
                     TRVPVSLVYRKGFKRDGSAAMLQYGYGSYGFSTDPAWSPTIPSLLDRGMVYAIAHVRG
                     GQEMGRAWYDQGRLLNKKNSFTDFIDVTRFLTGQGYAARDRVAAQGGSAGGLLMGAIA
                     NMAPNDYRVLIAQVPFVDVVTTMLDASIPLTTNEYDEWGNPAQKPFYDYMLSYSPYDN
                     VRAQAYPAMFVGTGLWDSQVQYYEPAKWVARLRAAKTDRNPLLFRVNMEAGHGGKSGR
                     FQRYRSIAEYYAFMLGQLGIGN"
     gene            complement(5672..7711)
                     /gene="ligA"
                     /locus_tag="VFU20_02320"
     CDS             complement(5672..7711)
                     /gene="ligA"
                     /locus_tag="VFU20_02320"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU5285329.1"
                     /translation="MAARLTEAEAAERLAWLAEAIARHDKLYHDQDEPEISDAQYDAL
                     VRENAAIEAQFPQLVRPDSPSRRLGATPTTALAKVAHARPMLSLENAFSEESVVEFVG
                     RVRRFLALPADESVAVTAEPKIDGLSCSLRYERGELALAATRGDGTVGEDVTPNARAI
                     ADVPQRIAGAPDVLEVRGEVFMSKADFAALNERQQSAGGKIFANPRNAAAGSLRQKDP
                     GVTAARPLRFLAHGWGELSEPLAATQFEAMRRIAAFGVPVSDLLERCADLDAMLSQYA
                     AIEHCRADLPFDIDGVVYKVDRIDWQERLGQVARAPRWGLAHKFPAEKAETTLEAIDI
                     QVGRTGKLTPVGRLKPVGVGGVIVANVTLHNRDEIARLGLRIGDRVRIQRAGDVIPQV
                     VENLTANEKRPPYQFPDHCPECGSEAVAAEGEVDVRCTGGLVCPAQRIERLKHFVSRG
                     ALDIDGLGEKTIVEFVELGWLNSPADIYRLAARRDELLEREGWKAKSVDNLLASIGAR
                     REPDPARFLFALGIRHVGIVTAKDLMRAFGSIESLRAAATGGNAQGELSLVEGVGPVV
                     AEALVDFFHEPHNRQVLDDLLSEVRPRPFVSQARQTEWSGKTIVFTGSLETMSRDEAK
                     AQAERLGARAAGSVSARTDLVVAGPGAGSKLKKAAELGVPVIGEDEWARIVAEAG"
     gene            complement(7713..8423)
                     /locus_tag="VFU20_02325"
     CDS             complement(7713..8423)
                     /locus_tag="VFU20_02325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5285330.1"
                     /translation="MTRETAVPLRFGDEELEGVLVARRDSARRPGVILFPTVMGVSDL
                     ELGFARQLVHLGYTGFVADLFGKAFRGAPRGVMMDELKRLRGDRASLRKRLLAVLESV
                     RSLDAVEPDRIVAIGYCFGGQCALDLARSGAGIAGVASFHGLLDPPGLPPQPMVAKVL
                     VFHGWDDPMAPPEAVVALGHELTEAGADWQIHAFGHVQHGFTNPGATGAVPGVVHNEL
                     AAERSWTALVNFLEELFG"
     gene            complement(8420..>9987)
                     /locus_tag="VFU20_02330"
     CDS             complement(8420..>9987)
                     /locus_tag="VFU20_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU5285331.1"
                     /translation="ETGAGKSILLDALGLALGARADAGLVRNGQDSAAVSAELDMPPG
                     HSVHALLAEHGLEPEPGEPLLLRRTLRADGGSRAFAGGAPVSAAVLRDIASVSVEVHG
                     QHDDRGLLNPKGHRELLDALGKVDAGPVAAAWAEVTRIERELGQARADAAEAARDRDW
                     LAFASAEIEALAPEPGEETRLAEERSAMQAGAKAGEALTGLDELLGGSDGALAKLRLA
                     ARRIERGAADHPLLAEALASLDRALIEACEGEDRIARAADALAFDPARLEAVEARLFE
                     IRALARKHRVEADALAALGDRMRGQLALIEEGGERIARLDAELVAAREAYAQAAQALT
                     ESRRAAAETIDSAVAAELAPLKLDSARFRTAIAPAEPGPAGTDRVEFEVSTNPGAPFG
                     PLTRIASGGELSRFILALKVALAEAGTAATMIFDEIDRGVGGAVASAIGERLARLASG
                     SQVLVVTHSPQVAARATHHYRIEKSHGPDGSRTSVRRLDEAERREEIARMLSGAFVTD
                     EARAQAARLLEAA"
BASE COUNT         1485 a         3516 c         3528 g         1458 t
ORIGIN      
        1 ctcaccgcag gcctcccagc accggcaccc agatgagcag ggtcaggacc agcgagccga
       61 ccagggtcag gatcgcgaac cgcttgttgg tgtcgagctc gaagccggcg ccgctgtcca
      121 tcaccagatg gcggatgccc gacagcagat gctggaagaa cgcccaggtc aggccgacca
      181 gcaccaccag cccgagcggg tgcgccgccg cggcggtgaa atcggcataa gcgtcggcgc
      241 cgtcggcgat cgccatcagc caccaggtca gcagcgcaag tccggcgatg gcgagggcgg
      301 cgccggtcat ccggtggacg atcgagacca gcattccggg gccccagcgc cagatggtca
      361 ggtgcgggga caacggccgc ggccggggcg aggattgatc ggtggccatt cgggcggggg
      421 cctttctgag ctcagcttga cgtccggcga acagggccgc gccggccgac gaccgcttag
      481 ggctcccacc gtgcggaggc aagaatggcg tccccctaac ccggtcttaa ccattgcggg
      541 ggcagaaagc ggcgacaagc gaagtatgcg gcgcaaggga aatgacggag accaaggaat
      601 gagcggcgac atcggacatc tgcagcgcct gaccaacgag caggtggcgg cgcgcattgc
      661 cgcctacggc gacgacggcc gcttcgaggc ttcgctgcgc cggctgtggg acgaagccgg
      721 atcagtggtc gaggcggcgg cgcgcagccg gttcggcgac gatgtcggtt gcctcggccg
      781 cgaccatttc acccggccgg tcgacgaggc ctgggtccgc tcggtcgccg agcagggcgt
      841 ccggatgttc gccgccaagc gcagcgtccc cgacttcatc gccgcccgcg cggcggtggc
      901 gaacgaagtg atccgagcct tcgacgagtg cttcgccggc cgctccgacg agcgcgtcga
      961 ggcgatcggc acgttccacc ggctgctcag cttcagcgag gacatcatcc tcgcccagat
     1021 cgctttgctc gaggccaatg aggccgccga ccagcgcggc cgccagagcg agcagttcga
     1081 gcgccgggtc gccgacttgg tccgagccag caccgagcag tcgaaggcgc tgaccgaccg
     1141 cacccgggcg accgccgcgt cggcccgcgg gatgctcggc aaaaccagcg aagtcgcggc
     1201 ggcggccgag cagtcggcgg tggcgatgcg cgaggccgcg cagaccgcag ccgggctgat
     1261 ccgggcgatc gaggacgcgc gttccgaagt cgagacggcg gcgggcgtcg cgatccgcgc
     1321 cggcggccag gccgagcagg cggtcaaggt gagccaggcg ctgtcgagcc acgtcgaggc
     1381 gatcgaatcg atcttgagcc tgatccgcga catcgccggc cagaccaatt tgctcgcctt
     1441 gaacgcgacc atcgaggcgg cgcgcgccgg cgatgccggg cgcggcttcg cggtcgtcgc
     1501 ccaggaagtg aagagcctcg cgtcgcagac agcccgggcg accgacgata tcaccaacaa
     1561 aatcaccgcc atccagcagg cgactcgcca gacggtcgag gccaacagct cgatccaggg
     1621 caccgtcgag gaggtccaga cctccgccga ccggatccgc gaggcgatgg agctgcaggc
     1681 gcagacggtg acgatgatca ccgcggcggt cgacgagacc gcgctcgccg ccgactcgat
     1741 gagctcgacg attgccgcga tccgctcgga caccgagaat gtcgccaagg acatcgacga
     1801 ggtcgagcat ggcttcggcc gcttcagcga gcagatctcc gacttcaagt cgaccaccaa
     1861 ggagttcgtc agcggcatcg ccgcctgacc tttcccgagc cgcgtctcgc cgctaggcgg
     1921 gacggatgaa catcctcgtc accggagcga gccgcgggat cggcgcggcg gcgttcgcct
     1981 tgctcaagtc caccgggcac cgcgtcgcgg gccactcaac caagggcggc ggcgaattga
     2041 tcgccggcga cctcgccgac gccgatgctc cgcgcgcatt atggaccgcg gcgctcgagc
     2101 ggctcggcgg gcggatcgac gtgctggtca acaacgccgg catctacgag gcggtcgccg
     2161 acgactcggc cgacggggaa tggcactcgg cgtggaaccg cacgctgacg atcaacctgc
     2221 aggcgccggc cgacctcagc cggctggcga tcggccattt ccgggagcgc ggcggcggcg
     2281 ggcgcatcgt caacatcgcc agccgcgccg cctttcgcgg cgattcgccg cagcactggc
     2341 attatgcggc gtccaaggcg gcgatgatcg ggatgacccg aaccatcgcc cgcggctacg
     2401 cggccgacgg gatcctcgcc ttcgcggtcg cgcccggctt caccgtgtcg gagatgaccg
     2461 acgaatatct cgccggccgc ggcggagcgc agatcgtcgc cgacatcccg ctggggcggg
     2521 tcgccaccac cgacgaggtc gcggaggcga tccgctggct ggcggccgac gcgccggcgt
     2581 cggcgaccgg cacggtgatc gacgtcaacg gcgccagcta cgtgcgttga cgcgatgagc
     2641 tggcgagcga ccctgccctg cacccgggtc caggccgaat cgctgcccga tgcccacgag
     2701 ttgctgccgc tcggcgattc gcagccggtg ctggtcgccg acgagcccga cccggcgcgg
     2761 ccgaacgaat ggctgctgca cgcgtatttc gaccaggagc cgtcggcgga ggtgctcgag
     2821 gcgctcgccc ggctcggttg cggcgccccc agcgtcgagc aattgggcga ggacgattgg
     2881 gtggcgatga gccagtcggg gctgcagccg atccgcgccg gccgcttctt cgtccatacg
     2941 ccgacccacc gcggcgccgc gcccagcggc agcatcgcgt tcgagatcga cgccagcctc
     3001 gcgttcggca ccggccagca cgcgaccacc tcgggctgcc tcgaagcgct tgattcgctt
     3061 gagcgcaacg gttgcacgtt tgcaaacatt gccgacgttg ggacgggtac cggtctgctc
     3121 gcattcgcgg cgctggcgct gtggcccgag tcgcgctgca tcggcaccga catcgactcg
     3181 gtcgcgatcg acgtcgcgcg cgacaatgcg gcgatcaacg gagtcgcgct cggacacggc
     3241 ccgggcgagc tgctgctcgc ggtcgccgac ggcatggact cgccgctgct gtcggcgcga
     3301 gcgccgttcg acctgatcat cgccaacatt ctcgccggcc cgctggtcga gctcgcgccc
     3361 agtttcgccg aagggctggc tgacgggggc accgtcatcc tcgccggcct gctcgacgcc
     3421 caggctgacg cggtcgcccg cgcctatcgc gacctcggcc tcgccttgga caagagcggc
     3481 acgggcgaat ggcccgtgct gacgctcgtc aagcgctagt tcccgatccc gagctggccg
     3541 agcatgaacg cgtaatattc ggcgatcgag cggtagcgct ggaagcgccc cgacttgccg
     3601 ccgtgccccg cctccatgtt gacccggaac agcagcggat tgcggtcggt cttggcggcc
     3661 cggagccgcg cgacccactt ggccggctcg taatattgga cctggctgtc ccacaggccg
     3721 gtgccgacga acatcgccgg ataggcctgg gcgcggacat tgtcgtacgg cgagtaggac
     3781 aacatgtagt cgtagaacgg cttctgcgcc gggttgcccc attcgtcata ttcgttggtc
     3841 gtcagcggga tcgacgcgtc gagcatcgtc gtcaccacgt cgacgaacgg cacctgcgcg
     3901 atcagcaccc gataatcatt gggcgccatg ttggcgatgg cgcccatcag cagcccgccg
     3961 gcgctcccgc cctgagcggc gacccggtcc cgggcggcat agccctggcc ggtcaggaag
     4021 cgggtgacgt cgatgaagtc ggtgaagctg ttcttcttgt tcaaaagccg accctggtca
     4081 taccaggcgc ggcccatttc ctggccgccg cggacgtggg cgatcgcata gaccatgccc
     4141 cggtcgagca ggctgggaat ggtcggcgac caggccgggt cggtcgagaa gccgtagctg
     4201 ccgtagccat attggagcat cgccgcgctg ccgtcgcgct tgaagccctt gcggtagacc
     4261 agcgagaccg ggaccctggt gccgtcgcgc gccggcgccc acaggcgctc ggtcacgtaa
     4321 tcggcggggt cgtaattggg cgacggggtg cgcttgagga cggtgcgagc gccggtccga
     4381 gaatccacct cgtaggtgat cttcggagtc gtcagcgaat cgtaggtgta gcgcagcctg
     4441 gtgctcgcga cctcgcgatt gtcgtcgagg ctcatggtgt aggcgggctc gtcggagccg
     4501 acgaggcggc tcctgccgct gttggcgagc agccggagcc gcttgttacc gccggcgcgc
     4561 tcctcgatgc cgatgaagct gctgaacggc tggaatccct cgatgaacac cttgggatcg
     4621 tgggggacga ggtcgctcca cgcgcttcgc ccggcggcgg ccgcggagtc cctcaccgtc
     4681 atcagccggt agttgggcgc gttccagttg gtccggatca cccagcggtc gccgacatgg
     4741 tcggcggtgt agaggaagtc gcgctcgcgc ggcgccagca cggcaaattc cccgggcgtg
     4801 gacgcgctgg tgcagcgctg ctcgttgctg accgtgctgc cgaggaagat gcagatgaac
     4861 cggtcgtcgc tggtccggcc gacgcccatg tagaagctgt cgtctttctc ctcgtagatg
     4921 aggcggtcgg ccgatgcggg agtgccgagc acgtgcgcct tgacgcgctt gctgagcagc
     4981 gtcaccgggt ccttctcgac ataatagagt gtgcggttgt cgtcggccca gacgaggttc
     5041 ggctcgacat tctcgacgac gtccgcaagc gccttgccgg tcgcgaggtc cttgaccttc
     5101 agcttgtact gccgccggcc gacgacgtcc tcggcatagg caagcagccg gttgtcctgg
     5161 ctgaccctat agtcgccgat ggcgaagaag ccgtggccct tggccatcgc cggctggtcg
     5221 agcagcacct gctcgggcgc cgtcatgctg ccgcggcggc gagcgaccac cggatagtcg
     5281 gcgccgacct cgaagcgggt gtagtaataa taaccgcgct cgcggaccgg gacgcttgaa
     5341 tcgtcctgct tgatacgccc gacgatctcc gaatagagct tgtcctcgag cggcctcaac
     5401 ggcgccagca ccgagtccgc gtaggcattc tcggccttga ggtaggcgag catctcgggt
     5461 tgcttgcggc tgtcgtcgcg cagccagtaa tattcgtcgg cgcggtcgag cgggcccttg
     5521 acggtgaacg gccgcttctc gacggccggc gggctcggcg gcgcggccgt ttgcgcctgc
     5581 accagcgccg gaacggcggc gagcgccagc agaatcgtcg atcgtttcat tcgcgatact
     5641 ccgggaaatt catgaaaatg cgcggccagg ctcagccggc ctcggccacg atcctagccc
     5701 actcgtcctc gccgatcacc gggactccga gctccgcggc tttcttgagc ttggagcccg
     5761 cgccgggccc ggcgacgacc aggtcggtcc tggcgctgac cgatcccgcc gcgcgcgctc
     5821 ctaggcgctc ggcctgcgcc ttggcttcgt cgcggctcat ggtctccagg ctgccggtga
     5881 aaacgatggt cttgccgctc cattcggtct ggcgggcctg gctgacgaac ggccggggcc
     5941 ggacttcgga cagcaggtcg tcgagcactt gccggttgtg cggctcgtgg aagaaatcga
     6001 ccagagcctc ggcgaccacc gggccgactc cttcgaccaa cgacagctcg ccttgcgcat
     6061 tgcccccggt cgcggccgcg cgcagcgact cgatcgagcc gaacgcgcgc atcagatcct
     6121 tggcggtgac gatgccgacg tggcggattc cgagcgcaaa caggaagcgc gccgggtcgg
     6181 gctcgcggcg ggccccgatc gaggcgagca gattgtcgac cgattttgcc ttccagccct
     6241 cgcgctccag cagctcgtca cggcgcgcgg ccaggcggta gatgtcggcc ggcgaattca
     6301 gccagcccaa ttcgacgaac tcgacgatgg tcttttcgcc gaggccgtcg atgtcgagcg
     6361 caccgcggct gacgaaatgc ttgaggcgct cgatgcgctg cgccgggcaa accaggccgc
     6421 cggtgcagcg gacgtcgacc tcgccctcgg cggcgaccgc ttcgctgccg cattcggggc
     6481 aatggtcggg gaattggtac ggcggccgtt tctcgttcgc ggtgaggttc tcgaccacct
     6541 gcggaatgac gtcgccggcg cgctggatcc gcacccggtc gccgatccga aggccgaggc
     6601 gagcgatctc gtcgcggttg tgcagcgtga cgttggcgac gatcaccccg ccgaccccga
     6661 ccggcttgag ccgcccgacc ggggtcagct tgccggtgcg cccgacctgg atgtcgatcg
     6721 cttccagggt cgtctcggcc ttctcggccg ggaatttgtg cgcgaggccc cagcgcggcg
     6781 cccgcgcgac ctggcccagt cgctcctgcc agtcgatccg gtcgaccttg tagaccaccc
     6841 cgtcgatgtc gaacggcaaa tcggcgcggc aatgctcgat cgccgcatat tgcgagagca
     6901 tggcgtcgag gtcggcgcac cgttcgagca ggtcgctgac cggcactccg aaagcggcga
     6961 tccgccgcat cgcctcgaac tgggtcgcgg cgagcggctc cgacagctcg ccccagccgt
     7021 gggcgaggaa gcgcagcggc cgcgcggccg tgacgcccgg gtccttctgg cgaagcgagc
     7081 ccgccgcggc gttgcgcggg ttggcgaaga tcttgccgcc cgccgactgc tgccgctcgt
     7141 tcaacgcggc gaagtcggcc ttggacatga acacctcgcc gcgcacctcg agcacgtccg
     7201 gggcgcccgc gatccgctgc gggacgtcgg cgatggcgcg cgcattcggg gtcacgtcct
     7261 cgccgaccgt gccgtcgccg cgcgtcgccg ccagcgccag ctcgccgcgc tcgtagcgca
     7321 gcgagcagga caggccgtcg atcttgggct cggcggtgac cgccaccgac tcgtcggccg
     7381 gcagcgccag gaaccgccgc acccggccaa cgaactcgac caccgactcc tccgaaaagg
     7441 cgttctcgag gctgagcatc ggccgcgcgt gggccacctt cgccaaagcc gtggtcggcg
     7501 tcgcgcccag ccgccgcgac ggcgagtccg gccggacaag ctgggggaat tgcgcctcga
     7561 tcgccgcgtt ctcgcggacc agcgcgtcat attgcgcgtc gctgatctcc ggctcgtctt
     7621 ggtcgtggta gagcttgtcg tggcgcgcga tcgcctccgc cagccacgcc agccgctcgg
     7681 cggcctcggc ctcggtcagc ctggcagcca tctcagccga acaattcctc gaggaagttg
     7741 accagcgcgg tccacgaccg ctcggcggcg agctcgttgt ggacgacgcc cggcaccgcg
     7801 cccgtcgcgc cggggttggt gaagccatgc tggacatggc cgaacgcgtg gatctgccag
     7861 tcggcgccgg cctcggtcag ctcgtggccg agcgcgacca ccgcctcggg cggcgccatc
     7921 gggtcgtccc agccgtggaa gacgaggacc ttggccacca tcggctgcgg cggcaggccg
     7981 ggcgggtcga gcagtccgtg gaagctggcg accccggcga tgccggcgcc gctgcgggcg
     8041 aggtccagcg cgcactggcc gccgaagcaa tagccgatgg cgacgatccg gtccggctcg
     8101 acggcatcga gcgagcggac ggactcgagc accgccagca ggcgcttgcg gaggctcgcg
     8161 cggtcgcctc gcaagcgctt caactcgtcc atcatgacgc cgcgcggggc gcctcggaac
     8221 gccttcccga acaggtcggc gacgaacccg gtgtagccca aatggaccag ctggcgggcg
     8281 aagccgagct cgaggtcgga gacgcccatc acggtgggga acaggatcac ccccgggcgg
     8341 cgggcgctgt cgcggcgcgc gaccagcacg ccttcgagct cctcatcgcc gaaccgcaag
     8401 ggaactgctg tctcgcgagt cacgccgcct ccagcagccg ggcggcctgg gcgcgggcct
     8461 cgtcggtgac aaacgcgccc gacagcatcc gggcgatctc ctcgcgccgc tcggcctcgt
     8521 cgagccggcg gaccgacgtc cggctgccgt ccggcccgtg cgatttttca atccggtaat
     8581 ggtgcgtggc gcgcgccgcg acctggggcg aatgggtgac gaccagcacc tgcgagccgc
     8641 tcgccagccg cgccaggcgc tcgccgatcg cgctcgccac cgctccgccc actccgcggt
     8701 cgatctcgtc gaagatcatc gtcgccgcgg ttccggcctc ggcgagcgcg accttcaaag
     8761 cgaggatgaa gcgcgacagc tcgccgccgc tggcgatccg ggtcagcggg ccgaacgggg
     8821 cgccggggtt ggtcgagacc tcgaactcga cccggtcggt gcccgcgggg ccgggctcgg
     8881 ccggcgcgat cgccgtccgg aagcgcgccg agtcgagctt cagcggggca agctcggcgg
     8941 caacggctga gtcgattgtt tcagcggctg cccggcggct ttcggtcagg gcttgagccg
     9001 cctgcgcata ggcctcgcgc gccgcgacca gctcggcatc gagccgggcg atgcgctcgc
     9061 cgccctcctc gatcagcgcc agctggccgc gcatccggtc gcccagcgcg gcgagcgcgt
     9121 cggcctcgac gcggtgcttg cgggcgaggg cccggatctc gaacagccgc gcctcgaccg
     9181 cctccaaccg cgccgggtcg aacgccagcg catcggccgc ccgggcgatc cggtcctcgc
     9241 cctcgcaggc ctcgatcaag gcccggtcga gcgacgccag cgcctcggcc agcaacgggt
     9301 ggtcggccgc gccgcgctcg atgcgccgcg ccgcgagccg caatttggcg agcgccccgt
     9361 ccgagccgcc gagcaattcg tcgaggccgg tcagcgcctc gcccgccttg gcgccggcct
     9421 gcatcgccga gcgctcctcg gcgagccgcg tctcctcgcc cggctcgggc gccagcgcct
     9481 cgatctcggc cgaggcaaaa gcgagccagt cgcggtcgcg tgccgcctcg gcggcgtcgg
     9541 cgcgcgcctg gccgagctcg cgctcgatgc gggtgacctc ggcccaggcg gccgcgaccg
     9601 ggccggcgtc gaccttgccg agcgcgtcga gcagctcgcg gtggcccttc gggttgagca
     9661 ggccgcggtc gtcgtgctgg ccgtggacct cgacgctgac ggaagcgatg tcgcgcagca
     9721 ccgcggcgga aaccggggcc ccgccggcga aggcgcggct gccgccgtcg gccctcaggg
     9781 tccgccgaag cagcagcggc tcgccgggct cgggctcgag gccatgctcg gcgagcaatg
     9841 catggaccga gtggccgggc ggcatgtcga gctcggcgga caccgctgcg gaatcctggc
     9901 cgttgcggac gagcccggcg tcggcccggg cgccgagcgc cagccccaat gcgtcgagca
     9961 ggatcgactt gccggcgccg gtctcgc
//
LOCUS       DASTZO010000086         2422 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_788571,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000086 DASTZO010000000
VERSION     DASTZO010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_788571"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VFU20_02335"
     CDS             <1..210
                     /locus_tag="VFU20_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285332.1"
                     /translation="EQDRLVATQEREALAEKAKVTEEQLDIDVRKPAEASAYAAVQQA
                     NAERDAANAAAEAEAFRRTYALLPR"
     gene            235..1416
                     /locus_tag="VFU20_02340"
     CDS             235..1416
                     /locus_tag="VFU20_02340"
                     /EC_number="2.6.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LL-diaminopimelate aminotransferase"
                     /protein_id="HEU5285333.1"
                     /translation="MDHDDFYRIRRLPPYVFAEVNAMKAAARARGEDVVDLGMGNPDG
                     APPKHVIDKLAEVAAKPNAHRYSASRGIPGLRKAQAAYYQRRFGVTVDPDTEVIVTLG
                     SKEGLANLAQAITAPGDVVLAPNPSYPIHSFGFIIAGAAVRSIPAAPGEDFFRRLDMA
                     MRYTVPRPKVLVIGYPSNPTAYVADLAFYERLVAFARTHELILISDLAYAEIYFGDVP
                     TPSILQVEGAKEVAVEFTSMSKTFSMAGWRIGFAVGNARLIEALARVKSYLDYGAFTP
                     VQAAATAALNGPQDIVDFNRALYRKRRDVLVECFGRAGWDIPVPQASMFAWAPIPEPF
                     RALGSMEFAKRLLTEAHVAVAPGVGFGEEGEGFVRIALVENEQRLRQAARGVKRMLAA
                     G"
     gene            1534..2040
                     /locus_tag="VFU20_02345"
     CDS             1534..2040
                     /locus_tag="VFU20_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285334.1"
                     /translation="MILLSAGAALLPVEKGISTGVIGALLLAAGLIEIVAGSMRRDTR
                     DLAMLAGGVTVLAGLLFLLNRESRFFPIVNIVIGWLALRSIILAVKSRRVEGSVRTWA
                     SVAAATDFLLALVLLVGLSLSTVVVLLFGPTPAVVASFAWIVALSFVATGLLLLEVAS
                     CEREAADS"
     gene            2051..>2422
                     /locus_tag="VFU20_02350"
     CDS             2051..>2422
                     /locus_tag="VFU20_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HEU5285335.1"
                     /translation="MFPTLAPAETGVLQVAQIIQLAVAPVFLLAGVGAFLNVCAGRLS
                     RIVDRARGLEPQILASRGSEHDRLLGELRILDQRMGLVSRAIFLSVLSALLICAVVVL
                     LFAASLTGAHFGTAVALLFIGS"
BASE COUNT          325 a          887 c          814 g          396 t
ORIGIN      
        1 gagcaggacc gcctcgtcgc gacgcaggag cgcgaggccc tcgccgagaa ggcgaaggtc
       61 accgaggagc agctcgacat cgacgtccgc aagccggccg aggcgtccgc gtacgccgcc
      121 gtccagcagg ccaacgccga gcgtgacgcc gccaacgccg ccgccgaggc cgaggcgttc
      181 cgccgcacct acgcgctctt gccgcgctga cccgccgccg ccatgagcgg cgcgatggat
      241 cacgacgact tctaccgcat ccgcaggctg ccgccctacg tgttcgccga ggtcaacgcg
      301 atgaaggcgg cggctcgggc gcgcggcgag gacgtcgtcg acctcggcat gggcaatccc
      361 gacggagcgc cgccgaagca tgtgatcgac aagctcgccg aggtcgccgc caagcccaac
      421 gcccaccgct attcggcgag ccgcggcatt cccggcctcc gcaaggcgca ggcggcctat
      481 taccagcgcc gcttcggcgt caccgtcgat cccgacaccg aggtcattgt caccttgggc
      541 tccaaggagg gcctcgccaa cctcgcccag gcgatcaccg ctccgggcga cgtcgtgctc
      601 gcgcccaacc cgagctaccc gatccacagc ttcgggttca tcatcgccgg cgccgcggtg
      661 cgctcgatcc cggccgcccc gggcgaagat ttcttccgcc ggctggacat ggcgatgcgc
      721 tacacggtgc cgcggcccaa ggtgctggtg atcggctatc cgtcgaaccc gaccgcttat
      781 gtcgccgacc tcgccttcta cgagcggctg gtcgccttcg cccggaccca cgagctgatc
      841 ctgatctccg acctcgccta cgccgagatt tacttcggcg acgtgccgac cccatcgatc
      901 ctccaggtcg agggcgccaa ggaggtcgcg gtcgagttca cctcgatgtc caagactttt
      961 tcgatggccg ggtggcggat cggcttcgcg gtcggcaacg cccggctgat cgaggcgctg
     1021 gcgcgggtca agtcctacct cgactatggc gccttcacgc ccgtccaggc ggccgcgacc
     1081 gcggccttga acggcccgca ggacatcgtc gatttcaatc gcgcgctcta caggaagcgc
     1141 cgcgacgtgc tggtcgaatg cttcggccgc gccggctggg acatcccggt gccgcaggcc
     1201 tcgatgttcg cctgggcgcc gatccccgag ccgttccggg cgctgggctc gatggagttc
     1261 gccaagcgcc tgctcaccga ggcccatgtc gcggtcgccc cgggcgtcgg cttcggcgag
     1321 gaaggcgaag gcttcgtgag gatcgcgctg gtcgaaaacg agcagcggct ccggcaggcg
     1381 gcgcgcggcg tgaagcggat gcttgcggcc ggctgacgga ccgcatggtt gccggccgcg
     1441 gattttgcta cacggcggcg cgtggaagcg actgaccagg acgccctctc gcatcgcaat
     1501 cgcgcgcggt ggatcagcat cgccggcgtg gcgatgatcc tgctcagcgc cggcgccgcg
     1561 ctgctgccgg tcgaaaaagg catctccacc ggagtgatcg gcgctctgct gctcgccgcc
     1621 ggcctgatcg agatcgtcgc cggctcgatg cgccgcgaca cgcgcgacct cgcgatgctc
     1681 gccggcgggg tcacggtcct cgccggcctg ctgttcctcc tgaaccggga gtcgcgattc
     1741 ttcccgatcg tcaacatcgt catcggctgg ctggcgctcc gcagcatcat cctcgcggtc
     1801 aagagccgcc gggtcgaggg gtcggtgcgc acctgggcga gcgtcgccgc ggcgaccgat
     1861 ttcctgctcg cgctggtgct gctcgtcggc ctgtcgctgt cgacggtggt cgtgcttctg
     1921 ttcggcccga cgcccgcggt cgtcgccagc ttcgcctgga tcgtcgccct gagcttcgtc
     1981 gccaccgggc tgctgctgct cgaagtcgcg agctgcgagc gcgaagccgc cgacagctag
     2041 caccaggcgg atgttcccga cgctcgcccc ggccgaaacc ggagtcctcc aggtcgccca
     2101 gatcatccag ctggcggtcg ctccggtgtt cctgctcgcg ggcgtcgggg cgttcctcaa
     2161 cgtctgcgcg gggcgcctgt cgcgaatcgt cgaccgggcc cgcgggcttg agccgcagat
     2221 cctcgccagc cgcggctccg agcacgaccg gctgctcggc gagctccgaa tcctcgacca
     2281 gcgcatgggc ctggtcagcc gggctatttt cctgtcggtg ctgtcggcgc tcttgatctg
     2341 cgcggtggtc gtgctgctgt tcgcggccag cctcaccggc gcccatttcg ggaccgcggt
     2401 cgcgctcctg ttcatcggct cg
//
LOCUS       DASTZO010000087         2077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_920353,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000087 DASTZO010000000
VERSION     DASTZO010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920353"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /locus_tag="VFU20_02355"
     CDS             <1..1608
                     /locus_tag="VFU20_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285336.1"
                     /translation="EVATTTNNAAAPAAEGAQPAAVAKLARVEMNPDTSFLTAEEREV
                     VNILIQAADLMSEIYKRQATPDYDRLRAEVAARNDRELLDRFDTFFGPWDSLEEDKPF
                     FGDRPRPPGANFYPADLTKEQFDSYLAAHPDEAEALTSPYTVVRRQGDRLVAVPYSQE
                     YRQWLEPAARLLEQAAAKTGNASLKKFLTLRAKAFRTDDYFESELAWMDLKDTPIEIA
                     IGPYEVYTDTLYGRKTAFEAFVTLRNPAESQALDVYKGHLRGMEENLPVEARYKNFQR
                     GFESPLVVADQVRGGGDNVPGVQTIAFNLPNDEKVREAKGAKKVILRNVLGAKYDRIL
                     KPMGSLVLVPDQAANVTQRYMFMETLFHELSHSLGPGTITVGGRKTTVDKELKDIAGG
                     FEEAKADVMGAYNVMHMMDKGVIPANERPQIRATYVAGLFRAMRFGTNEAHGKGAAMQ
                     YRYLRGKGAIVWDARAKRFRIDEAKIDGAIRDLVGEIVRRQGDGDYAGTKAFLDRWGS
                     LDAEASQVIGTMTHIPVDIRPVYPARI"
     gene            1611..>2077
                     /locus_tag="VFU20_02360"
     CDS             1611..>2077
                     /locus_tag="VFU20_02360"
                     /inference="COORDINATES: protein motif:HMM:NF025091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HEU5285337.1"
                     /translation="MTQAAAGRFKAFAALHVPGDPVVLYNIWDVGSAQAVVRAGAKAL
                     ATGSHPVADAHGWPDGQQVPIEFALANARRIVDAVEVPVTVDFEGAYSVDPAKGAANV
                     ARLAATGAVGCNFEDQVIGGADGGLHPLKEQAKRIEAIRLAVGDHFFINARTDL"
BASE COUNT          377 a          712 c          711 g          277 t
ORIGIN      
        1 gaggtcgcga cgaccaccaa caatgcggcc gcgccggcgg ccgagggagc gcagccggcc
       61 gcggtcgcca agctcgcccg ggtcgagatg aacccggaca ccagcttcct caccgccgag
      121 gagcgcgagg tggtcaacat cctgatccag gccgccgacc tgatgagcga gatctacaag
      181 cgccaggcaa ccccggacta cgaccggctg cgcgccgagg tcgcggcgag gaacgaccgc
      241 gagctgctcg accgcttcga caccttcttc gggccgtggg acagcctcga ggaggacaag
      301 ccgttcttcg gcgaccggcc caggccgccg ggcgcgaact tctacccggc cgacctcacc
      361 aaggagcagt tcgactccta cctcgccgcg cacccggacg aggccgaggc gctgaccagc
      421 ccgtacacgg tggtccggcg gcagggcgac cggctggtcg ccgtgcccta cagccaggag
      481 taccgtcagt ggctcgagcc ggccgccagg ctgctcgagc aggcggccgc gaagaccggc
      541 aacgccagcc tgaagaaatt cctgacgctt cgcgccaagg ccttccggac cgacgattat
      601 ttcgaatccg agctcgcctg gatggacctc aaggacaccc cgatcgagat cgcgatcggc
      661 ccgtacgaag tctacaccga cacgctttac ggccggaaga ccgcgttcga ggcgttcgtg
      721 acgctccgca acccggccga atcgcaggcg ctcgacgtct acaagggcca tttgcgcggg
      781 atggaggaga acctgcccgt cgaggcgaga tacaagaact tccagcgcgg tttcgaatcg
      841 ccgctggtgg ttgccgacca ggtccgcggc ggcggcgaca atgtcccggg cgtgcagacc
      901 atcgccttca acctgcccaa cgacgagaag gtgcgcgagg ccaagggcgc caagaaggtc
      961 atcctgcgca acgtgctcgg cgccaagtac gaccggatcc tgaagccgat gggcagcctg
     1021 gtgctggtcc ccgaccaggc ggccaacgtg acccagcgct acatgttcat ggagaccctg
     1081 ttccacgagc tgtcgcacag cctcggcccc gggacgatca cggtcggcgg ccgcaagacc
     1141 accgtcgaca aggagctcaa ggacatcgcc ggcggcttcg aggaagcgaa ggccgacgtg
     1201 atgggcgcct acaacgtcat gcacatgatg gacaaaggcg tgatcccggc caatgagcgg
     1261 ccgcagatcc gagcgaccta cgtcgccggc ctgttccggg cgatgcgctt cggcaccaac
     1321 gaggcccacg gcaagggcgc ggcgatgcag taccgctatt tgcgcggcaa gggcgcgatc
     1381 gtctgggacg cccgggcgaa gcgcttccgg atcgacgagg cgaagatcga cggcgcgatc
     1441 cgcgacctgg tcggcgagat cgtccggcgg cagggcgacg gcgattatgc cgggaccaag
     1501 gcgttcctcg accgctgggg gtcgctcgat gccgaggcca gccaggtgat cggcacgatg
     1561 acccacatcc cggtcgacat ccgcccggtc tatccggccc ggatctgacc gtgactcagg
     1621 ctgcggcagg ccggttcaag gcgttcgcgg cgctgcacgt cccgggcgac ccggtggtgc
     1681 tctacaacat ctgggatgtc ggcagcgcgc aggcggtcgt ccgcgccgga gcgaaggcgc
     1741 tcgccaccgg cagccacccg gtcgcggacg cccacggctg gcccgacggg cagcaggtgc
     1801 cgatcgaatt cgctctggcc aatgccaggc gcatcgtcga tgcggtcgag gttccggtga
     1861 cggtcgattt cgaaggcgct tattcggtcg acccggccaa gggcgcggcc aacgtcgccc
     1921 ggctggcggc gacgggagcg gtcggctgca acttcgagga ccaggtcatc ggcggcgccg
     1981 acgggggcct gcacccattg aaggagcagg cgaagcggat cgaggcgatc cggctggcgg
     2041 tcggcgacca cttcttcatc aacgcccgca ccgacct
//
LOCUS       DASTZO010000088        16126 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_57151,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000088 DASTZO010000000
VERSION     DASTZO010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 16126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16126
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57151"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..110)
                     /locus_tag="VFU20_02365"
     CDS             complement(<1..110)
                     /locus_tag="VFU20_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEU5285338.1"
                     /translation="MGFRCGIVGLPNVGKSTLFNALTQTAAAQAANYPFCT"
     gene            226..2703
                     /locus_tag="VFU20_02370"
     CDS             226..2703
                     /locus_tag="VFU20_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5285339.1"
                     /translation="MAKRKSDDRPIVLITGAAGNIGRSLAQLLADSYRVIGLDRPGLK
                     ADFPLIEADLADDKSIAKGITEFEKRFGKRVASVVHLAAYFDFTGEENPLYRSINVEG
                     TRRLLRELQRLEVEQFLYSGTMLVHAPGEPGRPIDEDQPIAPKWAYPKSKAETEEVIR
                     AEHGDIPYVLLHLAGLYDDETAVPTLSNQIARIYERDLQSYLYSGSTRVGQSMVHRDD
                     MLDAFQRAIDRRADIPPGTVILVGEEEAPGYASLQDEIGYLVHGADDWPTLRLPKPAA
                     AAGAWVQDKLEPLVPDALDQGEKPFVKPFMVALADDHYELDIARARKLLGWTPRHRIK
                     DTLPKIIAALKRDPKAWYEKNGLTPPPLVTEAQEAGRDPERVRAEHEALLVRQHRSSR
                     WAHLANIFLGTWLALQPPLIGVEEDWLATSEVMLGLATILFAALSLSWRLRAARWATA
                     GVGALVMAVPFLFWTENAAAYLSDTLVGGLIFAFAVGTRPDVGPSPLAATAGPVRPPG
                     WSYNPSAWSQRLPIILLAVVGLEISRYLAAYQLGHVETVWEPFFAGSPADPRNGTEEI
                     ITSHVSEAWPVSDAAVGGYTYALEILTGIVGSRARWRTMPWLVLLFGLMIAPLGVTSI
                     FFIIIQPILIGTWSTLALIAAAAMLIQIPYSLDELLATLQFLRRRVKAGRSWLRVFLF
                     GDTDEGRDEASPDEFGQGPGRFVADMWSGGVNLPWNLALAAGVGLWLMFTRLTLGADG
                     SMADADHLIGSLALTVISLAAAEVARPLRFLLVPLGAALLVTPFLFEASGAQRIASLL
                     CGIALMALSLRRGRIRARYGAWQSWIV"
     gene            complement(2707..3303)
                     /locus_tag="VFU20_02375"
     CDS             complement(2707..3303)
                     /locus_tag="VFU20_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02466 family protein"
                     /protein_id="HEU5285340.1"
                     /translation="MARALFATSLYEAEIGDSSLLDELAHSIRSLARDDAAGRRWSRE
                     HRYPGYTSYASLDDLPTRDPAFAALARLLARHAATFATDCAFDLPRKPRLDSLWANLL
                     KAGGRHGGHIHPHSMLSGTFYVEAPPGSGAIRFEDPRLGLMMAAPPRLADAPEELRTY
                     VEIEPRAGLLLLWESWLRHEVVPGRGKGGRLSVSFNFA"
     gene            3350..5116
                     /locus_tag="VFU20_02380"
     CDS             3350..5116
                     /locus_tag="VFU20_02380"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEU5285341.1"
                     /translation="MSDIFISYARSTEAEAEKVAEALRAKGYGVWRDDELPAHRTYAD
                     VIDERLKSAKAVVVLWSAEAAKSQWVRAEADAARQKGILVQASLDGTAPPIPFNQIQC
                     ADLKGWDGNPSAPGWKKVEGSVAALAGDAESDEKPAAKKARERSICVLPFANMSGEPE
                     QEYFSDGISEDITTDLSNISTLSVVARNTAFTFKGNAVDVGQVARKLGVSHVLEGSVR
                     KAGTRVRITAQLIDGATGDHLWAERYDRDLTDIFAIQDEISKAIVAALKLKLLPEEKK
                     AIEQRGTTSAEAYQLYLMARNYWVTGNWGDVRQLELVIRVCQRALEIDPNYGRAWGLL
                     AIVQSILFYTFSVGNDDGLAAAERALSLDSNIAEAYCVRARSSHEMGELDKADEALTQ
                     ALKLGPDSWEVNREAARIYYFQRRLNEAVRHYEKAVELDENDYHSWGMLASAYEALGN
                     RDGLLHAAKMVVTRSERVIAQDPTNAAALGTGASGLAILGERDRFDEWVERALLLSPD
                     NQIMRYNFACAIAVYFKDKDRSLDLLEPVFEWQPSNMLRNIVTDPDLDGLRGEPRFEK
                     MLETARRRMLADGIAPPIPTAT"
     gene            complement(5097..5675)
                     /gene="pth"
                     /locus_tag="VFU20_02385"
     CDS             complement(5097..5675)
                     /gene="pth"
                     /locus_tag="VFU20_02385"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU5285342.1"
                     /translation="MQIWAGLGNPGANYSLHRHNVGFMAADVIAEAHGFSAAKKAFRS
                     ELREGRIGRHRVLLLKPQVFMNRSGEAVAEALRFYKLDLDALTVFHDELDLAPMKVKV
                     KVGGGTAGHNGLRSIDAHLGPEFRRVRIGIGHPGPGQQEKVTPHVLGNYSKAEQEPLS
                     DLLAAVAAEAGWLADGDDARFMSEVALRLQSE"
     gene            complement(5768..6472)
                     /locus_tag="VFU20_02390"
     CDS             complement(5768..6472)
                     /locus_tag="VFU20_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEU5285343.1"
                     /translation="MSEQLTLPAEARERAGKGASRALRRDGRVPAVIYGDKKEPLSVH
                     VEEKLLAKMLSTGHFMNTVVMIDAGGEPHRTLPKDVQFHPVSSRPIHVDFLRIGEHSQ
                     VTVAIPVRFDDDEESPGIKRGGVLNIVQHELELVCPASQIPDEIHISLAGLDIGDSIH
                     ISQVSLPEGTRAAIDDRDFTVATIVAPSAVKAEEAEEAAAAAEEGEEVEGAEEAEGEG
                     EAGAEGEGGDKDSDKG"
     gene            complement(6693..7526)
                     /locus_tag="VFU20_02395"
     CDS             complement(6693..7526)
                     /locus_tag="VFU20_02395"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEU5285344.1"
                     /translation="MVHFLIAAAIAAASVSPAPAPAPAVPAIPDADPAMWVVNDHDTV
                     IYLFGTFHALDGKSAWFNDEVETAFKASDELVLETIVPERKREAPAKPSAAARRIPSM
                     PLTPSASFLASTRLAISAGRARGMHADFGADTVLRRAAQASGKPVRGLESFIAQLSMF
                     GRMPAQPAHTVAPRAGAPVQDPVAMENLAIVMGQMQSAWNRGDSDIFTMMLDQMRTNS
                     PESYNVMFPQRNSNWAGWIADRLQQPGTVFVAVGAGHLAGSDSVQAKLAELGIRSARI
                     N"
     gene            complement(7783..8682)
                     /locus_tag="VFU20_02400"
     CDS             complement(7783..8682)
                     /locus_tag="VFU20_02400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HEU5285345.1"
                     /translation="MLMRILRRGLAMLGLAALASCATTPPAEGATGRPALWQIADRDT
                     TIYLFGTIHLLPKDYPWRTAAFDKAVASSQTLVVETVVDNANPQALAAELARLGFRSG
                     LPPLADRVPPAKRALLETAIRKTGIPRPAFDRMETWAAAFMLLGTQFKDLGLTGEAGV
                     EAVLRNAFIQAGKPVGQLETNSEQLSLFDTLPESAQRSLLEGAIDSPAEMRSQFNEML
                     AAWARGDVAKIGATFNEDLSGSPELREALLKRRNVNWARWIQRRMAAPGSVFVAVGAG
                     HLAGPDSVVSMLQRDGYRVRRIQ"
     gene            8836..9702
                     /locus_tag="VFU20_02405"
     CDS             8836..9702
                     /locus_tag="VFU20_02405"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEU5285346.1"
                     /translation="MSFQDLILTLHRYWSAQGCVLLQPYDLEMGAGTFHPATVLRALG
                     PDSWKCAYVQPCRRPTDGRYGANPNRLGHYYQYQVMLKPSPPDLQQLYLGSLAEIGVD
                     FLKHDIRFVEDDWESPTLGAWGLGWEVWCDGMEVTQFTYFQQVGGFECRPVGGELTYG
                     LERLAMYVQGVDNVFDLAFNDSGVTYGDVFLDNEKQMSEWNFEVADTETLFDLFRKAA
                     AECENCLAARLAIPAYEQAIKASHLFNTLQARGVISVPERQAYIGRVRDLARAACAAW
                     LDAHGQPAGQAA"
     gene            9702..11804
                     /gene="glyS"
                     /locus_tag="VFU20_02410"
     CDS             9702..11804
                     /gene="glyS"
                     /locus_tag="VFU20_02410"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012051031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEU5285347.1"
                     /translation="MADFLLELLSEEIPARMQARARNDLARLFAEELDKAGLETGAVD
                     VHSTPRRLVLIARDVASETQAAREEIKGPKASAPPQALDGFLRKTGLEREQLEERDGV
                     LFAVVDRPGRPAAAVIGEALERIIAAFPWPKSMRWGGGETRWVRPLHGIVALLGEEIV
                     PVEVGGIASGATTLGHRFHHPGAITIGGAGDYAEKLRACHVIVDQDERERLIRDGAEK
                     AAADAGLKLLPDEGLVVENAGLAEWPVPLLGRFDSDYLDVPREVIVLTMRTHQKYFAC
                     TDGQGALAPAFVCVANIDARDGGAAIVEGNRRVLAARLADARFFWEQDLKVPLEAFLP
                     RLEAMLLYEDMGSLRAKADRIAELAAAICRTWFPERDAETARRAGLLAKADLATAMVG
                     EFPELQGIMGGHYAERGGEKPGVVSALRQQYLPCAEGCIPACVALADRIDTLASFFAK
                     GIKPTGSKDPFALRRAALAIIQTILANGTRMSLRDAFAMAEAKADVVELMDFVADRLK
                     VQQREAGVRHDLIDAVFALGGEDDLVRLLARVKALQEFVETGDGADLLVAYKRAANIL
                     KRENWTAPEIMSSPAEQGIPQTGEEDPLVLVEEPGMAEAVAEMASGSAGAALPQEQAL
                     VAALDSAEPQAVAAVEAEDFTGAMAALARLRGPIDAFFDHVTVNDPDPAARERRLNLL
                     LRFRDAVHRVADFSRIEG"
     gene            complement(11877..12146)
                     /locus_tag="VFU20_02415"
     CDS             complement(11877..12146)
                     /locus_tag="VFU20_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285348.1"
                     /translation="MIGFTSDQWVIVALVFILGMLIGAFLTAGGRRKWKARYRDAEAR
                     REAIEHELRDDRARWEEREKEWREQESLRGAAAKDRRDPADDRPL"
     gene            complement(12220..13209)
                     /locus_tag="VFU20_02420"
     CDS             complement(12220..13209)
                     /locus_tag="VFU20_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5285349.1"
                     /translation="MQAAGPTLLGFDVIWIATMLSAVATFAVLIAIYAATTVRDPMSR
                     RIKALNDRREQLKAGIVASTNKRKKLTNRNEAADRVRSILTSFKMIQDDQLKKTQLKL
                     MQAGIRTKDLAFFIIFARFVLPVVLGVTAVTMIYVLDYFPEWSWWRRYMTVAATLVGA
                     YKAPDVWLKNKVTKRSHAVRKGLPDALDLLVICAEAGLTVDAAFSRVAKELGKAYPEL
                     GDEFGLTAIELGFLNERRQAFENLANRVDLEAIRGVVTTMIQTEKYGTPLASALRVLS
                     AEFRNDRMMRAEEKAARLPAIMTVPLILFILPTLFIVILGPAACSINDSLLNG"
     gene            complement(13226..14194)
                     /locus_tag="VFU20_02425"
     CDS             complement(13226..14194)
                     /locus_tag="VFU20_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5285350.1"
                     /translation="MFYMVILFVLASAALLLIAKAYRGPSASKTLKRRVEMIRERHGD
                     VIAGHAQAQIRKLFAQRATRIESLASTLIPKPALLRRRLEMTGKDITLAKYGLISLGI
                     GLFVAAALTMRGAPLLLALFAGLFFGIGGPYFFIGKLIKRRIAKFNSNFPDAIELMVR
                     GLRSGLPITETLGIVASEISGPVGVEFRAVADKMKIGRTMEAALQETADRLGTPEFQF
                     FVITLAIQRETGGNLAETLSNLADVLRKRAQMKLKIRAMSSESKASAYIIGCLPFVVF
                     TLVWFINPTYMGGFFTDDRLIIAGLGGMVWMAIGGFIMAKMINFEI"
     gene            complement(14256..15524)
                     /locus_tag="VFU20_02430"
     CDS             complement(14256..15524)
                     /locus_tag="VFU20_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CpaE"
                     /protein_id="HEU5285351.1"
                     /translation="MNAPFQARAGLRDPFTAFVCDDATADMLRPVAVEHGWSPEKVNK
                     GGLRNAVQSLSISASPNILFVDLAESADPLNDINALAEVCEPGTIVIATGQVNDVRLY
                     RDLVASGIHDYLLKPFTVDQLRDTFAHAQQIISGPRGEAQADKPHVMTAVVGVRGGVG
                     ASTIATSLAWLLGERAHRSTALLDLDIHFGTGALALDLEPGRGLTDAIENPSRIDGLF
                     IERAMVRANERLSVLSAEAPIHQPLVTDGTAFFQLQEEMRNAFESTVVDLPRHMLIQY
                     PHMVHDAHVAVVVSELTLAATRDTIRILAWLKSNAPQTKVIVVANGVPSGGALEISRK
                     DFEQSIERPVDIVFPSDGKMAAQSAKLGKPMAEVATGKMAAPFNLLANMVLSHATEEG
                     AAGESKAMVGAKSLVDNLKSMLAKPKAKAA"
     gene            complement(15528..>16126)
                     /locus_tag="VFU20_02435"
     CDS             complement(15528..>16126)
                     /locus_tag="VFU20_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HEU5285352.1"
                     /translation="AVLSGCGHTPYDLPDRGVAAVNVPVVTRSDFVFDAAAPDGSLAP
                     GEAARLNAWFSGLDLGYGDAIYVDGPYAARSDVAQVAGYYGLMVAPSAPVTAGAVAPG
                     TARVIVSRNRASVPYCPNWSVPAQPNHQNRTMSNFGCAVNSNLAAMVANPEDLVHGRE
                     GTGVGDSLTSSKAIGAYRKAEPTGSKGLQDISTTKKDD"
BASE COUNT         2574 a         5285 c         5657 g         2610 t
ORIGIN      
        1 gtgcagaacg gatagttcgc cgcctgcgcc gccgcggtct gcgtcagcgc attgaaaagc
       61 gtcgatttcc cgacattcgg caagccgacg atgccgcagc gaaagcccat cgaaagtcct
      121 ttggtgaagg gaattgcgac gcgcttagcg gtgcgttcgg acaaattgaa gcgcttgcgc
      181 agggcgccgg ctgaaaccga ccgacgcgtt cgccgttggt cgtcggtggc gaagcgcaaa
      241 agcgacgacc ggccgattgt cctgatcacc ggggcggccg gcaacatcgg ccgctcgctg
      301 gcgcagctgc tcgccgattc ctatcgggtg atcgggctcg accggccggg cctgaaggcc
      361 gactttccac tgatcgaggc cgacctcgcg gacgacaagt cgatcgcgaa ggggatcacg
      421 gaattcgaga agcgcttcgg caagcgggtc gcgagcgtcg tccacctcgc cgcgtatttc
      481 gacttcaccg gcgaggagaa cccgctctac cggtcgatca acgtcgaggg gacgcgacgg
      541 ctgctgcgcg agctgcagcg cctcgaggtc gagcagttcc tctactcggg gaccatgctg
      601 gtccatgcgc cgggggagcc gggcaggccg atcgacgaag accagccgat cgcacccaaa
      661 tgggcctacc ccaagtcgaa agccgagacc gaggaggtga tccgcgccga gcacggggac
      721 attccctatg tgctgctcca cctcgccggc ctctacgacg acgagacggc ggtcccgacg
      781 ctcagcaacc agatcgcccg gatctacgag cgcgatttgc agagctatct ctactcgggc
      841 tcgacgcgcg tcggccagtc gatggtccac cgtgacgaca tgctcgacgc gttccagcgc
      901 gcgatcgacc ggcgcgccga catcccgccg ggcacggtga tcctggtcgg cgaggaagag
      961 gcgccgggct atgccagcct gcaggacgaa atcggctatc tggtccacgg cgccgacgac
     1021 tggccgacgc tacggctgcc caagcccgcc gccgcggccg gcgcctgggt ccaggacaag
     1081 ctcgagccgc tggtccccga cgcgctcgac cagggcgaga agccgttcgt caagccgttc
     1141 atggtggcgc tggccgacga ccattacgag ctcgacatcg cgcgcgcccg aaagctgctc
     1201 ggctggacgc caaggcaccg gatcaaggac acgctgccga agatcatcgc cgcgctgaag
     1261 cgcgacccga aggcctggta cgagaaaaat ggactgactc cgccgccgct ggtcaccgag
     1321 gcgcaagagg cgggccgcga ccccgagcgc gtccgcgccg agcacgaagc gctgctggtc
     1381 cggcagcacc gaagctcgcg ctgggcgcat ctggccaata tcttcctcgg cacctggctg
     1441 gcgctgcagc cgccgctgat cggcgtcgaa gaggattggc tcgcgacgag cgaagtcatg
     1501 ctcgggctcg ccaccatcct gttcgcggcc ttgtcgctgt cgtggcgcct gcgagcggcg
     1561 cgctgggcga ccgccggcgt cggcgcgctg gtcatggccg tgccgttcct gttctggacc
     1621 gaaaacgccg ccgcttacct ttccgacacg ctggtcggcg ggctgatctt cgcctttgcg
     1681 gtcggcacgc ggcccgacgt cggcccgtcg ccgctcgctg cgaccgccgg gccggtgaga
     1741 ccgccgggat ggagctacaa cccgtcggcc tggtcgcagc gcctgccgat catcctgctg
     1801 gcggtcgtcg ggctcgagat ctcgcgctac ctggcggcct accagctcgg ccacgtcgag
     1861 acggtctggg agcccttctt cgccggctcg cccgccgatc ccaggaacgg caccgaggag
     1921 atcatcacca gccatgtctc ggaggcctgg ccggtctccg acgcggcggt ggggggctac
     1981 acttacgcgc tcgagatatt gaccggaatc gtcgggtcgc gggcgcgctg gcgaaccatg
     2041 ccctggctgg tgctgctgtt cggcctgatg atcgcgcccc tgggcgtcac gtcgatattc
     2101 ttcatcatca tccagccgat cctgatcggc acctggagca cgctggcgct gatcgcggcg
     2161 gcggcgatgc tgatccagat tccctactcg ctcgacgaat tgctggcgac gctgcagttc
     2221 ctccggcgcc gggtgaaggc gggcaggagc tggcttcggg ttttcctgtt cggcgacacc
     2281 gacgagggcc gggacgaggc gtccccggac gagttcggcc agggcccggg ccgcttcgtc
     2341 gccgacatgt ggagcggcgg ggtcaacctg ccgtggaacc tggcgctcgc ggccggggtc
     2401 gggctgtggc tgatgttcac ccggctgacg ctcggcgccg acggctcgat ggccgatgcc
     2461 gaccatttga tcggctcgct ggcgctgacc gtgatctcgc tcgccgcggc cgaggtcgcg
     2521 cgcccgctgc gcttcctgct ggtgccgctc ggcgccgcgc tgctggtcac tccgttcctg
     2581 ttcgaggcga gcggcgcgca acggatcgcc agcctgcttt gcggcatcgc gctgatggcg
     2641 ctcagcctgc gtcgtgggcg catccgcgcg cgctacggcg catggcaaag ctggatcgtc
     2701 taggcgtcag gcgaagttga acgagacgct gaggcgcccg cccttgccgc ggccggggac
     2761 gacctcgtgg cgcagccagc tctcccacag cagcagcagg ccggcgcgcg gctcgatctc
     2821 gacgtaggtt cgcagctcct cgggagcgtc ggcgaggcga ggcggcgccg ccatcatcag
     2881 cccaaggcgc gggtcctcga accggatcgc gccggagccg ggcggcgctt cgacatagaa
     2941 agtgcccgac agcatgctgt gcgggtggat atgtccaccg tgccgtccac cggctttcaa
     3001 caggtttgcc cacaggctgt cgaggcgcgg cttgcgcggc aggtcgaacg cgcagtccgt
     3061 tgcaaatgtc gcggcgtggc gggcgagcag cctggcgagg gcggcgaacg ccgggtcgcg
     3121 cgtcggcaaa tcgtcgagcg acgcatagct ggtgtagccc ggataacggt gctcgcggct
     3181 ccagcggcgc ccggccgcat cgtccctcgc cagcgaccgg atcgaatggg cgagctcgtc
     3241 gagcagcgac gagtcgccga tttcggcttc gtagagcgac gttgcgaaca gggcgcgagc
     3301 catgactcgt cgatagagtc ctgatatcca tttcgaaagg gcggtcccag tgtcagatat
     3361 tttcatatcc tacgcgcgtt cgaccgaggc cgaggcggaa aaggtcgccg aggcactgcg
     3421 cgcgaagggc tacggcgtgt ggcgcgacga cgagctgccc gcgcaccgca cctacgccga
     3481 cgtcatcgac gagcggctga agtcggccaa ggcggtggtc gtgctctggt cggccgaagc
     3541 agcgaaatcg caatgggtgc gggccgaagc cgatgccgct cgccagaaag gcattctggt
     3601 tcaggccagc ctcgacggga cagcgccgcc gattccgttc aaccagatcc agtgcgccga
     3661 cctgaagggc tgggacggca atccatccgc gccggggtgg aagaaggtcg aggggagcgt
     3721 cgctgcgctg gccggcgatg ctgaatcgga tgagaagccg gcggcgaaga aggcacgcga
     3781 gcgctcgatc tgcgtgctcc cgttcgccaa catgagcggc gagcccgaac aggaatattt
     3841 cagcgacggg atcagcgagg acatcaccac cgatttgtcc aacatctcga cgttgtccgt
     3901 tgtcgccagg aacacggcgt tcaccttcaa gggcaacgct gtcgacgttg gccaggtcgc
     3961 tcgaaagctc ggggtcagcc atgtcctcga aggcagcgtc cgcaaggccg gaacccgggt
     4021 tcggatcacc gcccagctga tcgacggggc gaccggcgat catctctggg ccgagcgcta
     4081 cgaccgcgac ctgaccgaca tcttcgccat ccaggacgag atctccaagg cgatcgtcgc
     4141 ggccttgaag ctgaagctgc tgcccgagga gaagaaggcg atcgagcagc gcggcacgac
     4201 cagcgccgaa gcctatcaac tctatttgat ggcccgcaat tactgggtga ccggcaattg
     4261 gggcgatgtg cggcagctcg agctggtcat ccgcgtgtgc caacgcgcgc tcgaaattga
     4321 tccgaattat ggtcgggcct gggggttgct cgcgatcgtg caatccattc ttttctacac
     4381 cttttcggtc gggaacgatg acgggctagc ggccgcggag cgcgcgctgt cgctcgattc
     4441 gaacattgcc gaagcttatt gcgtgcgggc gcgcagctct catgagatgg gcgagctcga
     4501 caaggctgac gaggctctga cgcaggctct gaagcttggc ccggactctt gggaagtgaa
     4561 tcgcgaagcc gcccgcatct actatttcca gcgacgcttg aacgaggcgg ttcggcacta
     4621 tgagaaagcc gtcgagcttg acgaaaacga ctaccattca tggggaatgc tcgcatccgc
     4681 ctatgaggca ttgggcaatc gcgacggatt gctgcacgcg gccaagatgg tggtcactcg
     4741 gtcggaaagg gtaatcgccc aggaccccac caatgcggcg gctctgggaa ccggcgccag
     4801 cggactagcc attctcggcg aacgggaccg gtttgacgaa tgggttgaac gggctttgct
     4861 cctcagtccc gacaatcaaa tcatgcgata caatttcgcc tgcgcgatcg ccgtatattt
     4921 caaggacaag gaccgatcac tcgacctgct cgagccggtg ttcgaatggc agcctagcaa
     4981 catgctgagg aatatcgtga cggaccctga cctggacggg cttcgcggcg agccacggtt
     5041 tgaaaagatg ctggaaaccg cgcggcgccg aatgctcgcg gacgggatcg ctccgcctat
     5101 tccgactgca acctgagcgc gacctcgctc atgaagcgcg cgtcgtctcc gtcggcgagc
     5161 catccggcct cggcggcgac cgcggcgagc aggtcggaca agggctcctg ctcggctttc
     5221 gaataattgc cgagcacgtg cggggtgacc ttctcctgct ggcccgggcc ggggtggccg
     5281 atgccgatcc gcacccgccg gaattcgggg ccgaggtgcg cgtcgatcga gcgcaggccg
     5341 ttgtggccgg cggtgccgcc gccgaccttc accttgacct tcatcggtgc gaggtcgagc
     5401 tcgtcgtgga acacggtcaa agcgtcgagg tcgagcttgt agaagcgcag cgcctcggcg
     5461 accgcttcgc cgcttcggtt catgaagacc tgcggcttca gaagcaggac ccggtggcgg
     5521 ccgatgcggc cttcgcgaag ctcgctccgg aacgcctttt tcgcggccga aaagccgtgc
     5581 gcctcggcga tcacgtcggc ggccatgaag ccgacgttgt ggcggtggag cgagtaattc
     5641 gcgccgggat ttccgaggcc ggcccagatt tgcatcgccg cctcctagcg tgtcgcccgg
     5701 ccaaaaagaa accgcccgcc ccagggaaag ggcgggcggc tgttgagagt gatgggttgg
     5761 agcggactca gcccttgtcg ctgtccttgt cgccaccttc gccttcggct ccggcctcgc
     5821 cttcgccctc ggcttcctcg gcgccctcga cttcttcgcc ttcctcggcg gccgcggcgg
     5881 cttcctcggc ctcctcggcc ttgaccgccg acggagcgac gatcgtggcg acggtgaagt
     5941 cgcggtcgtc gatcgccgcc ctggtgccct cgggcaggct gacctggctg atgtggatcg
     6001 aatcgccgat gtcgagcccg gcgaggctga tgtggatctc gtcgggaatc tgcgaggccg
     6061 ggcagaccag ctcgagctcg tgctggacga tgttgagaac gccgccgcgc ttgatgcccg
     6121 gcgattcctc gtcgtcgtcg aagcggaccg ggatcgcgac cgtgacctgg ctgtgctcgc
     6181 cgatgcgcag gaagtcgacg tggatcggcc ggctgctcac cggatggaac tggacgtcct
     6241 tgggcagcgt gcggtgcggc tcgccgccgg cgtcgatcat caccaccgtg ttcatgaaat
     6301 ggccggtcga aagcatcttg gcgaggagct tttcctcgac gtgcaccgac agcggctctt
     6361 tcttgtcgcc gtagatcacg gcgggcaccc gtccgtcgcg ccgcaatgcc cgggaggctc
     6421 ccttgccagc ccgttcgcgc gcttcagcgg gcagcgtcag ctgttcgctc atgtgtcttc
     6481 actccaatgc aaaagccccg ccacgcctcc agggatgacc atggccgggg aagcgggcgc
     6541 ctatagggcg agcacgcgaa attggcaaat cctccccggg acgggcaggg ggaccatcct
     6601 cgcgaaggcg gggatggtgg aggggggttg tgccaggcgg agaaccccct ccgtcagcac
     6661 gacgtgctgc cacctccccg ttccggggag gattagttga tccgggcgga acggatgccg
     6721 agctcggcga gcttggcctg gacgctgtcg ctcccggcga ggtggccggc gccgacagcg
     6781 acgaacacgg ttccgggctg ctgcagccgg tcggcgatcc agccggccca attgctgttc
     6841 cgctggggga acatgacgtt gtagctctcg ggcgaattgg tccgcatctg gtcgagcatc
     6901 atcgtgaaga tgtcactgtc gccgcggttc cacgccgact gcatctggcc catgacgatc
     6961 gccagattct ccatcgcgac gggatcctgg accggcgccc cggccctggg agcgacggtg
     7021 tgggccggct gggcgggcat ccggccgaac atgctcagct gggcgatgaa gctctcgagg
     7081 ccgcggaccg gcttgcccga agcctgggcg gcgcggcgaa ggacggtgtc ggcgccgaaa
     7141 tcggcgtgca tgccgcgcgc ccggccggcc gaaatcgcca gccgggtcga ggcgaggaac
     7201 gaggcggacg gggtgagcgg catgctcggg atgcgccggg cggccgcgga cggcttggcg
     7261 ggcgcctcgc gcttgcgctc ggggacgatg gtctcgagga ccagctcgtc ggacgccttg
     7321 aaggcggtct cgacctcgtc gttgaaccag gccgacttgc cgtcgagcgc gtggaacgtg
     7381 ccgaacaggt agatcaccgt gtcgtggtcg ttgaccaccc acatcgccgg gtcggcgtcg
     7441 ggaattgccg gaacggcagg cgcgggagcg ggggcgggcg aaaccgatgc agcggctatc
     7501 gcggcggcga tcagaaaatg gaccatccct cgggtctccc cacccaaaga ccggcgccga
     7561 ggaagtggat gacggatgag cgcggctcgc tcgcatggcg caggacgccc tggactcgcc
     7621 ggccggcgcg aactgcttcg cgcccgccca tccgtgcatc gcgcccccca gcccgatacc
     7681 cggcgattat ggtcaccgag agattaacaa aagtctagcg caaagccgga tcgagtgaat
     7741 ttttgtcatt gcagttcagc aacaaaggcc gcgcttcggg gattactgga tgcgccgcac
     7801 ccggtagccg tccctctgca gcatcgagac gaccgaatcc ggtccggcga ggtggcccgc
     7861 tccgaccgcg acgaacaccg atccgggcgc ggccatgcgc cgctggatcc agcgcgccca
     7921 gttgacgttg cgccgcttca gcagcgcttc gcgaagctcg ggcgagccgg acagatcctc
     7981 gttgaaggtc gcgccgatct tggcgacgtc gccgcgcgcc caggcggcca gcatctcgtt
     8041 gaactggctg cgcatttcgg ccggggagtc gatcgccccc tcgagcagcg agcgctgggc
     8101 cgattccggc agcgtgtcga acaggctcaa ttgctcggaa ttggtctcga gctggccgac
     8161 cggcttgccg gcctggatga acgcgttgcg aagcacggcc tcgacgcccg cctcgccggt
     8221 caggccgagg tccttgaact gggtcccgag cagcatgaag gcggccgccc aggtctccat
     8281 ccggtcgaag gccgggcgcg ggatgccggt cttgcggatg gcggtctcga gcagcgcgcg
     8341 cttggccggc ggcacccggt cggcgagcgg cggcagcccg gatcggaagc ccaagcgcgc
     8401 cagctcggcg gccagcgcct gcgggttggc gttgtcgacg accgtctcga ccaccagggt
     8461 ctgcgagctc gccacggcct tgtcgaacgc cgccgtgcgc cacggatagt ccttgggcag
     8521 cagatggatg gtcccgaaca gatagatggt cgtgtcgcgg tcggcgatct gccacagcgc
     8581 cgggcggccg gtcgcgccct cggccggcgg ggtggtcgcg cagctcgcca gcgcggcaag
     8641 cccgagcatc gccagtccgc gccgcaggat cctcatcagc attgaacaca cctttcgtcg
     8701 cggatgcttg ggttgcgggc atgtagggag cctgaaccga cgcgtccact tgcccgtccc
     8761 gccgccatgc gccaaagccg cgctcggacc gaccggttgc gcgaagtgtt tgacggggag
     8821 cggcggcgct tggccttgag ctttcaggac ctgatcctca cgctccaccg ctactggagc
     8881 gcgcagggct gcgtgcttct ccagccgtac gacctggaga tgggggcggg gacgttccac
     8941 ccggcgacgg tgctccgggc gctcggcccc gactcgtgga aatgcgccta cgtgcagccc
     9001 tgccgccgcc cgaccgacgg gcgctacggc gccaacccca accggctcgg ccattattac
     9061 cagtaccagg tgatgctcaa gccgagcccg cccgacctcc agcagctcta cctcggcagc
     9121 ctcgccgaga tcggcgtgga tttcctcaag cacgacatcc gcttcgtcga ggacgattgg
     9181 gaaagcccga ccttgggcgc ctggggcctc ggctgggaag tgtggtgcga cgggatggag
     9241 gtcacccagt tcacctattt ccagcaggtc ggcgggttcg agtgccggcc ggtgggcggc
     9301 gagctgacct atggcctgga gcggctggcg atgtacgtcc agggcgtcga caacgtgttc
     9361 gacctcgcct tcaacgactc cggagtcact tacggcgacg tgttcctgga caatgagaag
     9421 cagatgtcgg agtggaattt cgaggtcgcc gacacggaaa ccctgttcga cctgttcagg
     9481 aaggccgcgg ccgagtgcga gaactgcctc gccgccaggc tcgcgatccc cgcttacgag
     9541 caggcgatca aggccagcca cctgttcaac acgctgcagg cgaggggagt gatctcggtc
     9601 cccgagcgcc aggcctatat cgggcgcgtc cgcgacctcg cccgggccgc gtgcgccgct
     9661 tggctcgacg cgcatggcca gccggcgggg caggcggcct gatggcggat ttcctgctcg
     9721 aattgctgtc cgaggaaatc ccggcgcgga tgcaggcgcg ggcgcgcaac gatctggcgc
     9781 ggctgttcgc cgaggagctc gacaaggcgg ggctcgagac gggcgccgtc gacgtccatt
     9841 cgaccccgcg gcggctggtg ctgatcgccc gcgacgtggc ctcggaaacc caggcggcgc
     9901 gcgaggagat caaggggccc aaggcttcgg ccccgcccca ggcgctcgac ggcttcctgc
     9961 gcaagaccgg gctcgagcgc gagcagctcg aggagcgcga cggggtgctg ttcgcggtgg
    10021 tcgaccggcc cggccggccg gcggcggcgg tgatcggcga ggctctggag cgcatcatcg
    10081 ccgccttccc gtggcccaag tcgatgcgct ggggcggcgg agagacccgc tgggtgcggc
    10141 cgctgcacgg catcgtcgct ttgctcggtg aggagatcgt gccggtcgag gtgggcggga
    10201 tcgccagcgg ggcgacgacg ctcggccacc gcttccacca tcccggggcg atcaccatcg
    10261 gcggcgccgg cgactatgcc gagaagctgc gcgcctgcca cgtgatcgtc gaccaggacg
    10321 agcgcgagcg gctgatccgc gacggcgccg agaaggccgc ggccgacgcc gggttgaagc
    10381 tgctccccga cgaggggctg gtggtcgaga atgccgggct ggccgaatgg ccggtgccgc
    10441 tcctcggccg cttcgactcc gattatctcg acgtgccgcg cgaagtgatc gtgctgacca
    10501 tgcgcaccca ccagaaatat ttcgcctgca ccgacgggca aggcgcgctc gccccggcgt
    10561 tcgtgtgcgt cgccaacatc gacgccaggg acggcggcgc ggcgatcgtc gagggcaacc
    10621 gccgagtgct cgccgcgcgg ctggccgacg cgcgcttctt ctgggagcag gacctgaagg
    10681 tgccgctgga agcgttcctg cccaggctcg aggcgatgct gctctacgag gacatgggca
    10741 gcctgcgggc gaaggccgac cggatcgccg agctcgcggc cgcgatctgc cggacctggt
    10801 ttcccgagcg cgacgccgag accgcccgcc gcgccggcct gctagccaag gccgatctcg
    10861 caacggcgat ggtcggcgag ttccccgagc tgcaaggcat catgggcggg cactatgccg
    10921 aacggggagg cgagaagccg ggagtggttt cggcgcttcg gcagcaatat ctgccgtgcg
    10981 ccgagggctg catcccggcc tgcgtcgcgc ttgccgatcg aatcgacacg ctggcctcct
    11041 ttttcgccaa agggatcaag ccgaccgggt ccaaggatcc gttcgcgctt cggagggcgg
    11101 ctttggccat catccagacc atcctcgcga acggaacgcg gatgagcctg cgcgacgcct
    11161 tcgcgatggc cgaagccaag gccgacgtcg tcgagctgat ggacttcgtc gccgaccggc
    11221 tgaaggtgca gcagcgcgag gcgggggtcc ggcacgacct gatcgacgcg gtgttcgcgc
    11281 tgggcgggga ggacgacctg gtgcggctgc tggcgcgcgt gaaggcgctg caggagttcg
    11341 tcgagaccgg ggacggcgcc gatctgctgg tcgcctacaa gcgggcggcg aacatcctca
    11401 agcgcgagaa ttggaccgca cccgagatca tgagctctcc ggccgagcag ggcatcccgc
    11461 agactggcga ggaggacccc ttggtgctgg tagaggagcc cgggatggcc gaggcggtcg
    11521 ccgagatggc gtcgggaagc gccggcgcgg ccctgccgca ggaacaggcg ctggtcgcgg
    11581 cgctcgattc ggccgagccc caggccgtcg cggcggtcga ggccgaggat ttcaccggcg
    11641 cgatggcggc gctcgcccgg ctgcgcgggc cgatcgacgc cttcttcgac catgtgacgg
    11701 tcaacgaccc cgacccggcg gcccgcgagc ggcggctgaa cctgctgctg cggttccgcg
    11761 acgccgtcca ccgggtcgcc gatttctcca ggatcgaggg gtaggccctc accccaaccc
    11821 tctcccgcgc gcgggagagg gaggaggaac gtagcgacgg agggtgaggg accttcttac
    11881 agcggccggt cgtcggccgg gtcgcgacgg tccttggccg cggcgccgcg cagcgactcc
    11941 tgctcgcgcc attccttctc gcgctcctcc caccgggcgc ggtcgtcgcg cagctcgtgc
    12001 tcgatcgcct cgcggcgcgc ctcggcgtcg cggtagcggg ccttccactt gcgccggccg
    12061 ccggcggtca ggaaagcgcc gatcagcatt cccaggatga agaccagggc gacgattacc
    12121 cactggtcgg acgtgaaacc gatcatcttc cgctcccctc ttttgaagac gcggggatac
    12181 agcgctcaag gcggcgctag cgttccaacg gtcgcgaaat cagccgttga gcaggctgtc
    12241 gttgatcgag caggccgccg gacccaggat gacgatgaac agggtcggca ggatgaacag
    12301 gatcagcggc acggtcatga tcgccggcag gcgcgcggcc ttttcctcgg cgcgcatcat
    12361 ccggtcgttg cggaactcgg ccgacagcac gcgcagcgcg gaggccagcg gagtgccgta
    12421 tttctcggtc tggatcatgg tcgtgacgac gccgcggatc gcttcgaggt cgacccggtt
    12481 ggcgaggttc tcgaacgcct ggcggcgctc gttcaggaag cccagctcga tcgcggtcag
    12541 cccgaactcg tcgccgagct ccggataggc cttgccgagc tccttggcga cccggctgaa
    12601 cgcggcgtcg acggtgagac cggcctcggc gcagatgacc agcaggtcga gcgcgtcggg
    12661 aagccccttg cggaccgcgt ggctgcgctt ggtgaccttg ttcttgagcc acacgtcggg
    12721 cgccttgtac gccccgacca gggtcgcggc gacggtcatg tagcggcgcc accacgacca
    12781 ttccgggaaa tagtcgagca cgtagatcat cgtcaccgcg gtgaccccga gcaccaccgg
    12841 cagcacgaag cgcgcgaaga tgatgaagaa ggcgaggtcc ttggtgcgga tgccggcctg
    12901 catcagcttc aattgggtct tcttgagctg gtcgtcctgg atcatcttga agctggtcag
    12961 gatcgaccgg acgcggtccg cggcctcgtt gcggttggtc agcttcttgc gcttgttggt
    13021 tgaggcgacg atgccggcct tcaattgctc gcggcggtcg ttgagcgcct tgatccggcg
    13081 cgacatcggg tcgcggacgg tggtcgcggc gtagatcgcg atcagcacgg cgaaggtcgc
    13141 gacggcgctg agcatcgtcg cgatccagat gacgtcgaaa ccgagaaggg tggggcctgc
    13201 cgcctgcatt gctatcgttt ccccgtcaga tctcgaagtt gatcatcttg gccatgatga
    13261 agccgccgat ggccatccac accatgcccc ccagaccggc gatgatcagc cggtcgtcgg
    13321 tgaaaaagcc gcccatgtag gtcgggttga tgaaccacac gagcgtgaag accacgaacg
    13381 gaaggcagcc gatgatgtaa gcggaggcct tcgactccga gctcatcgcg cggatcttga
    13441 gcttcatctg cgcgcgcttg cgaagcacgt cggcaagatt ggacaaggtc tcggcgaggt
    13501 tgccgccggt ctcgcgctgg atggccaggg tgatgacgaa gaactggaac tccggagtgc
    13561 ccagccggtc ggcggtctcc tggagcgcgg cttccatggt gcggccgatc ttcatcttgt
    13621 cggcgaccgc gcggaactcg accccgaccg ggcccgagat ctcgctggcg acgatcccca
    13681 gggtctcggt gatcggcagg cccgagcgaa ggccgcggac catcagctcg atcgcgtcgg
    13741 ggaagttgga attgaacttg gcgatgcgcc gcttgatcaa cttgccgatg aagaaatacg
    13801 ggccgccgat gccgaagaac aggccggcga agagggccag caggagcggc gccccgcgca
    13861 tcgtcaaggc ggcggccacg aacaggccga tgccgagcga gatcagcccg tatttggcca
    13921 gcgtgatgtc cttgccggtc atctccagcc ggcgccgaag cagcgccggc ttcgggatca
    13981 gggtcgacgc caggctttcg atgcgcgtcg cgcgctgcgc gaacagcttg cggatctgcg
    14041 cctgcgcgtg cccggcgatg acgtcgccgt ggcgttcccg gatcatctcg acgcgccgct
    14101 tgagcgtctt cgaggccgac gggccgcgat acgccttcgc gatcagcagc agcgccgctg
    14161 aggccaggac gaacaggatc accatgtaga acattcgtca gttccttcgg tgcggcaggg
    14221 cgggggtcgc cccccggggg cctgccgccg ttcgcctagg ccgccttcgc cttgggcttg
    14281 gccagcatcg acttcagatt gtcgaccaac gacttggcgc cgaccatcgc cttggattcg
    14341 ccggcggcgc cttcctcggt cgcgtggctg aggaccatgt tggccagcag gttgaacggc
    14401 gccgccatct tgccggtggc gacttccgcc atcggcttgc cgagcttggc ggactgcgcg
    14461 gccatcttgc cgtcgctcgg gaagacgatg tccaccggcc gctcgatcga ctgctcgaag
    14521 tccttgcggc tgatctccag agcaccgccc gagggaacgc cgttggcgac gacgatgacc
    14581 ttggtctgcg gggcgttgga cttgagccag gcgaggatcc ggatggtgtc ccgggtcgcc
    14641 gccagggtca gctccgacac taccaccgcg acgtgcgcgt cgtggaccat gtgcggatac
    14701 tggatcagca tgtggcgcgg caggtcgacg acggtcgatt cgaacgcgtt gcgcatttcc
    14761 tcctggagct ggaagaaagc ggttccgtcg gtgaccagcg gctggtggat cggcgcttcc
    14821 gccgacagca ccgacagacg ctcgttggcg cggaccatcg cccgctcgat gaacaggccg
    14881 tcgattcggc tcgggttctc gatcgcgtcg gtcaggccgc gcccgggctc gaggtcgagc
    14941 gccagcgcgc cggtcccgaa atggatgtcg aggtcgagca gcgcggtcga gcggtgggcc
    15001 cgctcgccga gcagccaggc cagcgaggtg gcgatggtcg atgcgccgac tccgccgcga
    15061 accccgacca ccgccgtcat gacgtgcggc ttgtcggcct gggcctcgcc gcgcggtccg
    15121 gaaatgatct gctgcgcatg ggcgaaggtg tcgcgcagct ggtcgaccgt gaacggcttc
    15181 aggagatagt cgtggatgcc gctggcgacc aggtcgcggt agaggcggac atcgttgacc
    15241 tgcccggtgg cgatcacgat cgtgcccggc tcgcagactt cggcgagcgc gttgatgtcg
    15301 ttcaagggat cggccgattc cgccaggtcc acgaacagga tgttcgggct tgccgagatc
    15361 gacagcgact gcaccgcgtt gcgcaggccg cccttgttga ccttctccgg cgaccagccg
    15421 tgctcgaccg cgaccggccg gagcatgtcg gcggtggcat cgtcgcacac gaaagccgtg
    15481 aatggatcgc gcagacccgc gcgtgcctgg aacggagcgt tcatcgccta gtcgtccttt
    15541 ttcgttgtgc tgatgtcctg gagccccttg gagccggtcg gctccgcctt gcggtaggcg
    15601 ccgatcgcct tcgaagaggt cagggaatcg ccgaccccgg tgccctcgcg cccgtggacc
    15661 aggtcctcgg gattggcgac catcgccgcg aggttggagt tcaccgcgca gccgaagttg
    15721 gacatcgtcc ggttctggtg attgggctgc gccggaacgc tccaattggg gcagtaaggg
    15781 acgctcgcgc ggttgcggct gacgatcacc cgggcggtcc ccggggcgac ggcgccggcg
    15841 gtgaccggcg cgctcggagc gaccatcagg ccgtaatagc cggcgacctg agcgacgtcg
    15901 gaccgggccg catagggacc gtcgacgtag atcgcatcgc cgtagccgag gtcgaggccg
    15961 ctgaaccagg cgttgagccg ggcggcctcg ccgggagcca gcgagccgtc gggagcggcc
    16021 gcgtcgaaca cgaagtcgga gcgggtcacg accgggacgt tgactgccgc gactccgcgg
    16081 tccggcaggt cgtagggcgt gtggccgcag ccggagagca ccgcgg
//
LOCUS       DASTZO010000089         2306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1155562,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000089 DASTZO010000000
VERSION     DASTZO010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155562"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1063
                     /locus_tag="VFU20_02440"
     CDS             <1..1063
                     /locus_tag="VFU20_02440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5285353.1"
                     /translation="VVVWEEPIDIGPKETPFLQVREAEGFANVRVAVPHLPEGASEER
                     REESLRRLLDAQVASLNGPLVAWYYTPMMLDFSRHLDASAVVYDCMDELSKFRFAPEK
                     LLDLEQELIDKADLVFTGGSSLYEAKKHRHDSVHLFPSSVDRAHFAKARGGLFDPADQ
                     EELPRPRLGFYGVIDERFDIELLDTVAGMRPDWSLVMVGPVAKIAPEDLPRRPNIHYL
                     GPKTYAELPAYLAGWDVALMPFAMNESTQFISPTKTPEYLAGGKPVVSTPVRDVVRHY
                     GLLEGVRIAGDAESFVAACEQALELARCPTGKWLAEADLMLSATSWQTTQARMAGLLS
                     EVLGLRIGHRAPATLVAAE"
     gene            1060..2238
                     /gene="glf"
                     /locus_tag="VFU20_02445"
     CDS             1060..2238
                     /gene="glf"
                     /locus_tag="VFU20_02445"
                     /EC_number="5.4.99.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008767 - UDP-galactopyranose mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009273 - peptidoglycan-based cell wall
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
                     /protein_id="HEU5285354.1"
                     /translation="MNAMSPAPRSDRPHYDYLIVGAGFAGSVLAERLASQHKARVLLI
                     DRRDHVGGNAYDEKDAAGILYHKYGPHIFHTNSEQVVDYLSQFTEWRPYEHRVLAQVR
                     GQLVPIPINRTTLNKLFGLDLRTDEEAAAYLASRAEPVDDIQTSEDVVVNAVGRELYE
                     LFFRGYTRKQWGLDPSELDKQVTSRIPTRTNTDDRYFGDTFQAMPKDGYTAMFLAMTD
                     HPLIEKALGTDFRDIRGRIDAGHVIYTGPIDEYFDFRFGKLPYRSLRFDHRTLDQDQF
                     QPVAVVNYPSEDVPYTRISEYKHLTGQAHPRTTITYEYPSAEGDPYYPIPRPENQLLF
                     KRYEALADATEGVTFVGRLATYRYYNMDQIVGQALATFRRMEERRQPAVRLQAAAAAA
                     "
BASE COUNT          400 a          809 c          740 g          357 t
ORIGIN      
        1 ggtggtggtc tgggaagagc cgatcgacat cggccccaag gaaacgccct ttctccaggt
       61 ccgcgaggcc gagggcttcg ccaacgtccg cgtggccgtc ccccatcttc ccgagggcgc
      121 cagcgaagag cgccgcgagg aaagcttgcg gcggcttctc gacgcgcaag tcgccagcct
      181 caacggcccg ctggtcgcct ggtactacac gccgatgatg ctcgacttct cgcgccatct
      241 cgacgccagc gcggtcgtct acgactgcat ggatgaattg agcaagttcc gcttcgcgcc
      301 cgagaagctg ctcgatctcg agcaggaact gatcgacaag gcggacctcg tcttcaccgg
      361 cggctcgagc ctgtacgagg ccaagaagca ccgccacgat agcgtccatc tgttcccgtc
      421 gtcggtcgac cgcgcccatt tcgccaaggc gcgcggcggt ctgttcgacc ccgccgacca
      481 ggaagagctg ccgcgcccgc ggctgggctt ctacggggtg atcgacgagc ggttcgacat
      541 cgagcttctc gacacggtcg ccgggatgcg gcccgactgg tcgctggtga tggtcggccc
      601 ggtcgccaag atcgcgcccg aggacctgcc gcggcggccc aacatccatt atctcgggcc
      661 aaagacctac gccgagctgc ccgcctatct cgccggctgg gacgtcgctt tgatgccgtt
      721 cgcgatgaac gaatcgacgc agttcatctc gccgaccaag acgcccgaat atctcgccgg
      781 cggcaagccg gtggtctcga ccccggtccg ggacgtggtg cgccactacg gcctgctcga
      841 gggcgtgcgg atcgccggcg acgccgagag cttcgtcgcc gcgtgcgaac aggcgctgga
      901 gctcgccaga tgcccgaccg gcaagtggct cgccgaggcc gacctgatgc tgtcggcgac
      961 cagctggcag accacccagg cgcggatggc cgggctgctt tccgaagtgc tcgggttgcg
     1021 catcggacac cgggcgccgg cgacgctggt ggcggcggaa tgaacgccat gagccccgcc
     1081 ccgcgcagcg accgtccgca ttacgactat ctgatcgtcg gcgccggttt cgccggctcg
     1141 gtcctcgccg agcggctggc gagccagcac aaggcgcgcg tcctgctgat cgaccgccgc
     1201 gatcatgtcg gcggcaacgc ctatgacgag aaggacgcgg ccgggatcct ctaccacaag
     1261 tacggcccgc acatcttcca caccaacagc gaacaggtag tcgactatct ctcgcagttt
     1321 accgagtggc ggccgtacga gcaccgcgtg ctcgcgcagg tccgcggcca gctggtgccg
     1381 atcccgatca accgcacgac cctcaacaag ctgttcgggc tcgacctccg gaccgacgag
     1441 gaggccgcgg cctacctcgc ttcgcgggcc gagccggtgg acgacatcca gacctccgag
     1501 gacgtcgtcg tcaacgccgt cggccgcgag ctctatgaat tgttcttccg gggctacacc
     1561 cgcaagcagt gggggctcga cccgagcgag ctcgacaagc aggtgacgtc gcggatcccg
     1621 acccgcacca acaccgacga ccgctatttc ggcgacacct tccaggcgat gccgaaggac
     1681 ggctacaccg cgatgttctt ggcgatgacc gatcatccgc tgatcgagaa ggcgctcggc
     1741 accgactttc gcgacatccg cggccggatc gatgccggcc acgtcatcta caccgggccg
     1801 atcgacgaat atttcgactt ccgcttcggc aagctgccgt accggagcct caggttcgac
     1861 caccggacgc tcgaccagga ccagttccag ccggtcgcgg tggtcaatta cccgtccgag
     1921 gacgtgccct acacgcggat cagcgaatac aagcatctga ccgggcaggc gcacccgagg
     1981 acgaccatca cctacgaata tccgtccgcc gagggcgatc cttactatcc gatcccgcgg
     2041 ccggagaatc agttgctgtt caagcgctac gaagcgctcg ccgacgcaac cgagggagtg
     2101 acgttcgtcg gccgcctcgc cacctaccgc tactacaaca tggaccagat cgtcggccag
     2161 gcgctggcga cgttccggcg gatggaagag aggcggcagc ccgcggtgcg gctgcaggcc
     2221 gcggccgcag ccgcataggg acggcggcgc gcagcccctg tcggccgcga ttcccggtgg
     2281 gcaagctggt tgcttccggg gcggat
//
LOCUS       DASTZO010000090         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_267979,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000090 DASTZO010000000
VERSION     DASTZO010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267979"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..716
                     /locus_tag="VFU20_02450"
     CDS             <1..716
                     /locus_tag="VFU20_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5285355.1"
                     /translation="LLDCWADAPTEALFAADAARLEAVRADRAEAASRYFSAQAEVWD
                     SIRSLHVAESEVEQAIERALEEKELGRLVDIGTGTGRMIELFGPRAAQAIGIDRSSEM
                     LRLARVKLEAAGIASSLRQGDMYALPLADGSADSVIIHQVLHYAHSPAAAIAEAARVL
                     SPDGTLLVVDFAAHEREELRASDAHIRLGFDDEVMAGWFASAGLALDHVEHLEGGELT
                     VTLWRGVKGAVPQRQRRAA"
     gene            713..1627
                     /gene="metF"
                     /locus_tag="VFU20_02455"
     CDS             713..1627
                     /gene="metF"
                     /locus_tag="VFU20_02455"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEU5285356.1"
                     /translation="MNRHPIGGAADCKPLFAEARGDIAVSFEFFPPKTEAMAETLWRS
                     IETLAPLRPRFVSVTYGAGGSTRERTHATVERIVRETDLTAAAHLTCVGATREEIDAI
                     ARGYWDIGVRHLVALRGDPPEPGQKYRPHPGGYANAAELVAGLKKVAPFDISVAAYPE
                     VHPDSRDRADDLDNLRRKIDAGADRAITQFFFSADCFLRFRDEVAAAGIDVEMVPGIL
                     PVSNVATTRRFAGQCGATIPQWLDELFEGLDELPSARQLIAATVAAELCGQLYAAGVR
                     HFHFYTLNRAELSYAICHLLGVRAEAAT"
     gene            1624..>2622
                     /locus_tag="VFU20_02460"
     CDS             1624..>2622
                     /locus_tag="VFU20_02460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HEU5285357.1"
                     /translation="MSAADALRAEAAKRILVKDGAYGTSVQRRRLASDDYRGELELAK
                     DQRGNNDLLNLTRPDLVEEIAGEFAEAGADILATNTFNANAISQADYGAEALVADINR
                     AAARIIRSVADRHSERDGRPRWVAGALGPTNKTLSLSPDVNDPAYREVDFDTVKSVYR
                     EQIDALVEGGVDFILIETIFDTLNAKAGIKAALEAADALGRPLPLMISMTLTDLSGRN
                     LSGHTVEAFWASVRHAKPVTVGLNCSFGAAQLRPHLAALAARADTLVMAYPNAGLPNE
                     LGEYDEAAAETAAQVSEWIADGLVNIVGGCCGTTAEHIAAIASAARSGAPRRAPVPD"
BASE COUNT          385 a          955 c          915 g          367 t
ORIGIN      
        1 ccttgctcga ttgctgggcg gatgcgccga ccgaggcgct gttcgcggcc gacgcggcgc
       61 ggctggaggc ggtgcgcgcc gaccgcgccg aggccgcgtc gcgctatttc tccgcccagg
      121 cggaggtgtg ggattcgatc cgctcgctgc acgtcgccga aagcgaggtc gagcaggcga
      181 tcgagcgcgc gctcgaggag aaggagctcg gccggctggt cgacatcggc accggcaccg
      241 gccggatgat cgaattgttc gggccgcgcg cggcccaggc gatcggcatc gaccgctcgt
      301 ccgagatgct gcgcctcgcc cgggtcaagc tcgaggccgc gggcatcgcc tcgagcctgc
      361 gtcagggcga catgtacgct ctgccgctgg ccgacggcag cgccgacagc gtcatcatcc
      421 accaggtgct ccattacgcc cactcgccgg ccgcggcgat cgccgaggcg gcgcgggtcc
      481 tgtcgcccga cgggactctg ctggtcgtcg atttcgccgc ccacgagcgc gaggagctgc
      541 gggccagcga cgcccacatc cgcctgggct tcgatgacga ggtgatggcc ggctggttcg
      601 catcggcggg cctcgccctc gaccatgtcg agcatctcga gggcggcgag ctgaccgtca
      661 ccctgtggcg cggggtcaag ggcgcggttc cgcagcgcca gcggagggcg gcgtgaaccg
      721 ccacccgatc ggcggcgcgg ccgactgcaa gcccttgttc gccgaggcgc gcggcgacat
      781 cgccgtcagc ttcgaattct tcccccccaa gaccgaggca atggccgaga ccctgtggcg
      841 gtcgatcgag acgctggcgc cgctccggcc gcgcttcgtg tcggtcacct acggcgccgg
      901 cggatcgacc cgcgagcgca cccacgcgac ggtcgagcgg atcgtccgcg agaccgacct
      961 caccgccgcc gcgcatctga cctgcgtcgg cgccacccgc gaggagatcg acgcgattgc
     1021 ccgcggctat tgggacatcg gcgttcggca cctggtcgcg cttcgcggcg acccgcccga
     1081 gccggggcag aaataccggc cgcacccggg cggctacgcc aatgccgccg agctggtcgc
     1141 gggcctgaag aaggtcgccc cgttcgacat ttcggtcgcc gcctaccccg aggtccaccc
     1201 ggacagccgc gaccgcgccg acgacctcga caatctgagg cgcaagatcg acgccggagc
     1261 cgaccgggcg atcacccagt tcttcttctc cgccgactgc ttcctgcgct tccgcgacga
     1321 ggtcgcggcg gccgggatcg acgtcgagat ggtgcctggc atcctgccgg tgtccaacgt
     1381 cgcgaccacc cggcgcttcg ccggccagtg cggcgcgacc atcccgcaat ggctcgacga
     1441 gctgttcgaa ggcctcgacg agctcccgtc ggcgcgccag ctgatcgccg cgaccgtcgc
     1501 cgccgagctg tgcggccagc tctacgcggc cggcgtccgc cactttcact tctacacgct
     1561 caatcgcgcc gagctgtcct acgcgatctg ccatctgctc ggagtccggg cggaggccgc
     1621 cacatgagcg ccgccgatgc cctgcgcgcc gaggcggcca agcgcatcct ggtcaaggac
     1681 ggcgcctacg ggacgtcggt ccagcggcgg cggctggcga gcgacgatta tcgcggcgag
     1741 ctcgagctgg ccaaggacca gcgcggcaac aacgaccttc tcaacctcac ccggcccgat
     1801 ctggtcgagg aaatcgccgg cgagttcgcg gaagcgggcg ccgacatcct cgccaccaac
     1861 acgttcaacg ccaacgcgat cagccaggcc gactatggcg ccgaagcgct tgtggcggac
     1921 atcaaccggg ccgcggcgcg gatcatccgc tcggttgcgg accgccattc cgagcgcgac
     1981 ggccggccgc gctgggtcgc cggggcgctc ggcccgacca acaagacgct ctcgctgtcg
     2041 cccgacgtca acgaccccgc ctaccgcgag gtcgatttcg acacggtgaa atccgtctac
     2101 cgcgagcaga tcgacgccct ggtcgagggc ggggtcgact tcatcctgat cgagaccatc
     2161 ttcgacacgt tgaatgccaa ggctgggatc aaggcggcgc tcgaggcggc ggacgcgctc
     2221 ggccggccgc tgccgctgat gatctcgatg accctgaccg atttgagcgg ccgcaacctg
     2281 tccggccaca cggtcgaggc gttctgggcg tcggtccggc acgcgaagcc ggtgacggtc
     2341 gggctcaact gctcgttcgg ggcggcgcag ctgcggccgc atttggcggc gctggcggcg
     2401 agggccgaca cgctggtgat ggcctatccc aacgccggcc tgcccaacga gctcggcgaa
     2461 tatgacgagg cggcggccga gacggcggcg caggtatccg agtggatcgc cgacgggctg
     2521 gtcaacatcg tcggcggctg ctgcgggacc accgccgagc atatcgccgc gatcgcctcg
     2581 gccgcgcgca gcggggcacc gcgcagggcg cccgttccgg ac
//
LOCUS       DASTZO010000091         2893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1051578,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000091 DASTZO010000000
VERSION     DASTZO010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051578"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1004)
                     /locus_tag="VFU20_02465"
     CDS             complement(159..1004)
                     /locus_tag="VFU20_02465"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5285358.1"
                     /translation="MSREPARNVDPATVRGFGEEWSANDQSGLQGAEYRKLFDGYFAI
                     FPFDALPEGAEGFDLGCGSGRWAAGVAPRVGTLHCIDPSEEALAVARRRLADLPNVRF
                     HLASVDSIPLEDGSQDFGYSLGVLHHVPDTAGALEDCVRKLKPGAPFLLYLYYKLEGR
                     PAWFRAIWRATELGRQVIARLPFPARKAATTAIAALVYWPLARIAGAGERLGLDVAHV
                     PLSAYRYRSFYSMRTDALDRFGTRLEQRFSRDEIAAMMRKAGLADIGFSDREPFWVAL
                     GRRVA"
     gene            complement(1001..1891)
                     /gene="rfbD"
                     /locus_tag="VFU20_02470"
     CDS             complement(1001..1891)
                     /gene="rfbD"
                     /locus_tag="VFU20_02470"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEU5285359.1"
                     /translation="MKVLVTGTEGQLVRSMIEKRAGWPEIELLAVGRPEADLEVPGSV
                     ARAIEARRPDAVVNAAAFTAVDQAEDEPARATRINADAAGEAAAAARSIGAPIVQLST
                     DYVFSGDEPGALSEDSPTGPIGTYGRSKLAGEEQVRAANPDHLIVRTAWVYSPFGRNF
                     VRSMVKAAEDRDTLSVVDDQRGSPTSALDLAGAILHVLDQWRRGGRTGLGETYHVAGS
                     GETSWCGLAEEIMRRCRELGLPAARVVPIKTSDWPTRARRPANSVLDCAKFECDFGLR
                     LPEWQGSVGEVVARLAEQVR"
     gene            complement(1888..>2893)
                     /gene="rfbB"
                     /locus_tag="VFU20_02475"
     CDS             complement(1888..>2893)
                     /gene="rfbB"
                     /locus_tag="VFU20_02475"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019054745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEU5285360.1"
                     /translation="HLVASGGYQVVNVDKLTYSGSLESLRDVADSPDYSFHRQDICDQ
                     GAMLDIFRREKIDRVMHLAAESHVDRSIDGPAVFLKTNVEGTFALLQAALQYWRELGT
                     DAAAAFRFHHVSTDEVFGDLPFDEGVFTEETPYAPSSPYSASKAASDHLVRAWHATYG
                     LPVVLSNCSNNYGPFHFPEKLIPLIILNGLEGNRLPVYGRGANVRDWLYVEDHARALE
                     LVLASGRVGESYNVGGRSERTNLQVVEAVCDLLDSRRPLDNGRSHRELIEFVADRPGH
                     DRRYAIDPAKIERELGWRAQESFESGLAKTVDWFLDNEWWWRPIREQKYAGARLGTGG
                     "
BASE COUNT          435 a         1004 c         1026 g          428 t
ORIGIN      
        1 ggcgtcgccc aggtcggcgg gcttctcgaa gctgagcaga tggacctcat ggtcccgcgc
       61 cagcggctcg agatagctca gcacctggga ctggccgagc ggctcgagca ttccgtcgta
      121 gctgatgtac agaatggcgg ggcgcggcat ggggcggatc aggcgacccg gcgcccgagc
      181 gcgacccaga acggctctcg gtcgctgaag ccgatgtcgg ccaggcccgc cttgcgcatc
      241 atcgcggcga tctcgtcgcg cgagaagcgc tgttcgagcc gggtcccgaa ccggtcgagc
      301 gcgtcggtcc gcatcgaata gaagcttcgg tagcgatagg cgctcaatgg gacatgcgcg
      361 acgtcgaggc caaggcgttc gccggccccc gcgatcctcg ccagcggcca gtagaccagg
      421 gccgcgatcg ccgtggtcgc cgccttcctc gccgggaacg ggagcctggc gatgacctgc
      481 cggccgagct cggtggcgcg ccagatcgcc cggaaccagg ccggccgccc ctcgagcttg
      541 taatagaggt agagcaggaa cggggcgccc ggcttgagct tgcgcacgca atcctcaagc
      601 gcccctgccg tgtcggggac atggtggagg actcccagcg aatagccgaa atcctggctg
      661 ccgtcctcga gcgggatcga atcgacgctg gcgaggtgaa agcggacatt gggaaggtcg
      721 gccaggcggc ggcgggcgac cgccagcgcc tcctcggacg ggtcgatgca gtgcagcgtg
      781 ccgacccgcg gcgcgacccc cgccgcccag cggcccgagc cgcagcccag gtcgaagccc
      841 tccgcgccct cgggaagcgc gtcgaacggg aagatcgcga aatagccgtc gaacaatttc
      901 cggtactcgg cgccctggag ccccgactgg tcgttggccg accattcctc gccgaatccc
      961 cggacggtgg ctggatcgac attgcgggcg ggctcgcggc tcatcgcacc tgctccgcga
     1021 gccgggcgac gacttcgccg accgagcctt gccattccgg cagccggagc ccgaagtcgc
     1081 actcgaactt ggcgcagtcg aggaccgagt tggcgggccg ccgggcgcgg gtcggccagt
     1141 cggacgtctt gatcggcacc acccgcgccg cgggcaggcc gagctcccgg cagcggcgca
     1201 tgatctcctc ggcaaggccg caccagctgg tctcgcccga cccggcgaca tgataggtct
     1261 cgccgagccc ggtgcggccg ccgcggcgcc actggtcgag cacatggagg atcgctccgg
     1321 cgaggtcgag cgccgaggtc gggctgccgc gctggtcgtc gaccacgctc aacgtgtcgc
     1381 ggtcctcggc cgccttgacc atgctgcgga cgaaattgcg gccgaacggg ctgtagaccc
     1441 aggcggtgcg gacgatcaga tggtcggggt tggccgcgcg gacctgctcc tcgccggcga
     1501 gcttcgagcg gccgtaggtc ccgatcggcc cggtcggcga atcctcggac aaggcgcccg
     1561 gctcgtcgcc gctgaacaca tagtcggtcg acagctggac gatcggagcg ccgatgctcc
     1621 gcgccgccgc cgccgcctcg cccgccgcgt cggcgttgat ccgcgtggcc cgcgcgggct
     1681 cgtcctcggc ctggtcgacg gcggtgaagg cggccgcatt gacgacggcg tctggccggc
     1741 gcgcctcgat cgcccgggcg acgctgcccg gcacctcgag gtccgcttcg ggacggccga
     1801 cggcgagcag ctcgatttcg ggccagccgg cgcgtttctc gatcatgctt cggacaagct
     1861 ggccttcggt gcccgtgacg agaaccttca tccgccggtc ccgagccggg cgccggcata
     1921 tttctgctcg cggatcgggc gccaccacca ttcattgtcg aggaaccagt cgacggtctt
     1981 cgcgagcccg ctctcgaagc tctcctgcgc ccgccagccg agctcccgct cgatcttggc
     2041 gggatcgatc gcatagcggc ggtcgtggcc cggacggtcc gcgacgaatt cgatcagctc
     2101 acggtggctg cggccgttgt cgagcggccg cctggagtcg agcaggtcgc agaccgcctc
     2161 gaccacctgg aggttggtgc gctcggagcg gccgccgaca ttgtagctct cgccgacccg
     2221 gccgctcgcc agcaccagct cgagcgcccg cgcatggtcc tcgacgtaca gccagtcgcg
     2281 gacgttggcg ccgcggccgt agaccggcag ccggttcccc tcgaggccgt tcaggatgat
     2341 cagcgggatc agcttctcgg ggaagtggaa cggaccgtaa ttgttcgagc agttcgaaag
     2401 cacgacgggc aggccgtagg tcgcgtgcca cgcgcgcacc agatggtcgg acgccgcctt
     2461 cgacgccgag taaggcgacg acggggcgta gggcgtctcc tcggtgaaca cgccctcgtc
     2521 gaacggcagg tcgccgaaca cctcgtcggt cgagacgtgg tggaagcgga aggccgcggc
     2581 ggcgtccgtc cccagctcgc gccaatattg gagcgccgcc tggagcaacg cgaacgtgcc
     2641 ctcgacgttg gtcttgagga acaccgccgg cccgtcgatc gagcggtcga catggctttc
     2701 cgcggcgagg tgcatcaccc ggtcgatctt ctcgcgccgg aagatgtcga gcatcgcgcc
     2761 ctggtcgcag atgtcctgcc ggtggaacga ataatcgggg ctgtcggcca cgtcccggag
     2821 cgactcgagg ctgcccgagt aggtcagctt gtcgacgttg acgacctggt atccgcccga
     2881 cgcgacgagg tgg
//
LOCUS       DASTZO010000092         4036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1077501,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000092 DASTZO010000000
VERSION     DASTZO010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077501"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..452
                     /locus_tag="VFU20_02480"
     CDS             <1..452
                     /locus_tag="VFU20_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285361.1"
                     /translation="VDYVRLIPDGLLPEVSVYAPFKAVVVIEATYSPEWQDEVSRWLV
                     DSGCRYMMAWGDNCSSWDDSVDHAEILKHLPGDVPDNQFVMTTWHKDETLENVFWYAQ
                     FCAQFSYDDVALTNALIIHISETVREAEMRALFEQSKTLAEREPEEG"
     gene            complement(449..1159)
                     /locus_tag="VFU20_02485"
     CDS             complement(449..1159)
                     /locus_tag="VFU20_02485"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HEU5285362.1"
                     /translation="MASARIRNIVVLTGAGVSAESGVATFRGPDGLWEGHRVEDVATP
                     EAYRRDPALVHAFYDARRAKLGTIGPNAAHEALARLDSEWPGELLLVTQNVDDLHERA
                     GSRRLLHMHGQLKRGWCLECDERFAWDGAMGEGAVCPACARPGRVRPDIVWFGEMPYE
                     MERIDDALQRCDLFVSIGTSGAVYPAAGFVQTARYCGARTLELNLEPSQGSVFFDESR
                     IGRAGELVPAWVEEVLGG"
     gene            1352..1765
                     /locus_tag="VFU20_02490"
     CDS             1352..1765
                     /locus_tag="VFU20_02490"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEU5285363.1"
                     /translation="MEKELRGIETQWNADYNKRDVDAVMGHYAEDAALANPGMALAAA
                     SESRRAAITQFLADPNLEISFASDRVQVAKSGDLAYTRGHYTMQGTDPATKQPRTDTG
                     NYLTVWQKQTDGSWKAVEDFVVPGGSAAAAEPAAG"
     gene            1852..2349
                     /locus_tag="VFU20_02495"
     CDS             1852..2349
                     /locus_tag="VFU20_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013836903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEU5285364.1"
                     /translation="MSKNLLMLAAAALLLPACEKAGRTDSVEATQAETAADESAIRAI
                     NQRWLELIRNKDSAAIAQLYAEDAAVMPANEKAAQGRQAIGDWWNRSMQMPGYDLTFS
                     TDQLLLSKSSDMALDRGTYRFAATPPSGAISDTGKYVVVWRKVGGEWKVAADIFNSDL
                     PAAGG"
     gene            2584..3021
                     /locus_tag="VFU20_02500"
     CDS             2584..3021
                     /locus_tag="VFU20_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285365.1"
                     /translation="MKKWIIGAAMLASAFTSAPALAQQSSYKLGPMWTAARIDVEDGQ
                     YENYLDWLTKVWVGNQAFAKSQGWILDYHILDSVNPRDGEPDLILITRFADFPSVAEI
                     DRRNAIMNERMKQDDHAADAASGQRKAMRRQMGSVLYRELLKR"
     gene            3086..3796
                     /gene="dapB"
                     /locus_tag="VFU20_02505"
     CDS             3086..3796
                     /gene="dapB"
                     /locus_tag="VFU20_02505"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEU5285366.1"
                     /translation="MRDPEQPIRITLFAPNGRMGQAIAAAAAEDPGFAIDPDHGDVLV
                     DFSAPSALQASLDRATSAGVPILIGTTGLDDFAGRRIAAAAEQVAVLRAANTSLGIAL
                     LADLVERAAGLLGPEWDVEIVEAHHRTKADSPSGTALVLGEAAARGRGTELKAERGRD
                     GTGLKRGDGAVGFASVRGGTVVGDHDVLFLGPDERLVLAHRAESRAIFARGALAAARF
                     LVAQPPGLYSMRDVIDAV"
     gene            3807..>4036
                     /locus_tag="VFU20_02510"
     CDS             3807..>4036
                     /locus_tag="VFU20_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU5285367.1"
                     /translation="MNKAQVFEFYSRLAEANPAPTTELEYHNPYTLLVAVVLSAQATD
                     AGVNLATRELFRRVRTPRQMVALGEPALREQI"
BASE COUNT          710 a         1383 c         1338 g          605 t
ORIGIN      
        1 gagtcgacta tgtgcggctg attccggacg gattgttgcc agaggtttct gtctacgctc
       61 cctttaaggc ggtcgtggtg atcgaagcca cttattcacc agaatggcag gatgaagtca
      121 gccgatggct cgtcgacagc ggctgccgtt acatgatggc ctggggggat aattgcagca
      181 gctgggatga ttccgtcgat cacgcagaaa tcttgaagca cctacctggc gacgtccctg
      241 acaatcaatt tgtcatgacg acctggcaca aggatgagac gctcgaaaac gtgttctggt
      301 atgctcaatt ctgtgcgcag tttagctatg atgacgtagc gctgacgaac gcgctgatca
      361 tccatattag cgaaaccgtt cgagaagcgg agatgagggc gctcttcgaa cagtcgaaaa
      421 cgctcgctga gcgagaaccg gaagagggtt aaccgcccag aacctcttcg acccatgccg
      481 gcaccagctc gccggccctg ccgatccggc tctcgtcgaa gaacacactc ccctggctcg
      541 gctcgaggtt gagctcgaga gtccgggcgc cgcagtagcg cgcggtctgg acgaagcccg
      601 cggccggata gaccgcgccc gaggtgccga tcgacacgaa caggtcgcag cgctggagag
      661 cgtcgtcgat gcgctccatc tcgtacggca tttcgccgaa ccagacgatg tcggggcgga
      721 cccggcccgg cctggcgcag gcggggcaaa ccgctccctc acccatggcc ccgtcccagg
      781 cgaaccgctc gtcgcactcc aggcaccagc cgcgcttcaa ttggccgtgc atgtgcagca
      841 gccgccgcga tcccgcgcgc tcgtggaggt cgtcgacgtt ctgggtgacc agcagaagct
      901 cgcccggcca ttcggagtcg agccgcgcca gcgcctcgtg cgcggcgttc ggcccgatcg
      961 tgccgagctt cgcccgccgc gcgtcgtaga aggcgtgaac cagcgccggg tcgcgccgat
     1021 acgcctcggg cgtcgcgacg tcctcgaccc ggtgcccttc ccacagcccg tccggcccgc
     1081 ggaaggtcgc gaccccgctt tccgccgaca ctccggcgcc ggtcaggacg acgatgttgc
     1141 gaatccgagc ggacgccatg acgtccgccc ctagccaagc gcattcgctt gcgcgactca
     1201 ccgcggcgtg gttgtttcgc cgctggacca atcgagcagg ggcatcgcca tgggaaacgt
     1261 caccagaatc gcgctcgcgg catcagcgct catggtgctt tcgggctgcg atcaagcccc
     1321 gccgaaaccg ccggccacgg acgcggcggc ggtggaaaag gaattgcgcg gcatcgagac
     1381 gcaatggaac gccgactaca acaagcgcga cgtcgatgcg gtgatgggcc attatgccga
     1441 ggacgcggcg ctcgccaatc ccggcatggc gctcgccgcg gccagcgagt cgcgccgcgc
     1501 cgcgatcacc cagttcctgg ccgacccgaa cctcgagatc tccttcgcca gcgaccgggt
     1561 gcaggtcgcg aagtcgggcg atctcgctta cacgcgcggc cattacacga tgcaggggac
     1621 cgacccggcg accaagcagc cgcgcaccga caccggcaat tatctgaccg tatggcaaaa
     1681 gcagacggac ggaagctgga aagcggtcga ggacttcgtc gtccccggcg ggtcggccgc
     1741 ggcggccgaa ccggctgcgg gatagcgatg cggcgcacgc cagcgacgat cgaaggccgg
     1801 aagcttcgcc ggtgcgctcg aaatgatacg accagcaaca aggagaggac gatgtcgaag
     1861 aacctgctga tgcttgccgc ggccgcattg ctcctgccgg cctgcgagaa ggccggccga
     1921 accgactcgg tcgaggcgac ccaggcggag actgccgcgg acgagagcgc catccgcgcc
     1981 atcaaccagc gctggctgga gctgatccgg aacaaggatt cggcggcaat cgcgcagctt
     2041 tacgccgagg acgccgcggt catgccggcg aacgagaaag cggctcaggg ccggcaggcg
     2101 atcggcgact ggtggaaccg ctcgatgcag atgccgggct acgatctgac cttctcgacc
     2161 gaccagctgc tgttgtccaa gtccagcgac atggcgctcg accgcggcac ctaccgcttt
     2221 gccgccacgc cccccagtgg cgcgatcagc gacaccggca aatatgtcgt cgtgtggcgc
     2281 aaggtcggcg gcgaatggaa ggtcgcggcg gacatcttca acagcgacct gccggccgcc
     2341 ggaggctgat cgatcgccag ggcgttgccg tgcaagccgc cgatccggcg ctcagagacc
     2401 ggctggggat aatcgcataa ccctccggcc tgtttaattt ttgttaggag cgactcggcg
     2461 ccccgttgca taggcccgtc ccggggccgt gattcggacg ctccctgtcg acttgcgtca
     2521 gcaccggtcc tgaatggccg gcagcgccgc cggattcccg gcggcttcct tgggggaaag
     2581 ccaatgaaaa agtggatcat cggcgcggcc atgctggcca gcgccttcac cagcgcaccg
     2641 gcgctggcgc aacaaagcag ctacaagctg ggaccgatgt ggaccgcggc ccggatcgac
     2701 gtcgaggacg gccaatatga gaactatttg gactggctga ccaaggtttg ggtcggcaac
     2761 caggcgttcg ccaagtcgca gggctggatc ctcgactatc acatcctcga cagcgtcaac
     2821 ccgcgcgacg gcgagcccga cctcatcctg atcacgcggt tcgccgactt cccgtcggtt
     2881 gcggaaatcg accggcgcaa cgcgatcatg aacgagcgca tgaagcagga cgaccatgcg
     2941 gccgacgcgg ccagcggcca gcgcaaagcc atgcgcaggc agatgggcag cgtcctctac
     3001 cgcgagctgt tgaagcgctg agcggggcgg agccggtgca cggcgcggat tgcgcgtgca
     3061 ccggcgctgc gccatgagcg cggccatgcg tgaccccgag cagccgatcc gcatcactct
     3121 gttcgcgccc aacggacgga tgggccaggc gatcgccgcg gccgccgccg aagatcccgg
     3181 cttcgcgatc gacccggacc atggcgacgt gctggtcgat ttctcggccc cgtcggcgct
     3241 ccaggcgagc ctcgaccggg cgacatccgc cggggtgccg atcctcatcg gcaccaccgg
     3301 gctcgacgat ttcgctgggc gccggatcgc cgccgccgcc gagcaggtcg cggtgctacg
     3361 ggccgccaac acgtcgctcg gcattgccct gctcgcggac ctcgtcgagc gcgccgcggg
     3421 cctgctcggg ccggagtggg acgtcgagat cgtcgaggcg caccaccgga cgaaggccga
     3481 ctcaccgtcc ggcaccgcgc tggttctcgg cgaagccgcg gcccgcggcc gcggcaccga
     3541 gttgaaggcc gagcgcggcc gcgacggcac cggcctcaag cgcggcgacg gagcggtcgg
     3601 cttcgcctcg gtccgcggcg gcaccgtcgt cggcgatcac gacgtgctgt ttctggggcc
     3661 cgacgagcgg ctggtgctcg cccaccgcgc cgaaagccgg gcgatcttcg cccgcggagc
     3721 cctggccgcg gcgcggttcc tcgtcgccca gccgcccggc ctctactcga tgcgcgacgt
     3781 catcgacgcc gtttgaatcg cgagacgtga acaaggccca ggttttcgaa ttctactcgc
     3841 ggctggccga ggccaatccg gcgccgacca ccgagctcga atatcacaac ccctacacgc
     3901 tgctggtcgc ggtcgtgctg tccgcccagg cgaccgacgc cggcgtcaac ctcgccaccc
     3961 gcgagctgtt ccggcgcgtg cgcacgcccc ggcagatggt cgcgctcggc gagccggcgc
     4021 ttcgcgagca gatcaa
//
LOCUS       DASTZO010000093         5360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_163718,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000093 DASTZO010000000
VERSION     DASTZO010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163718"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1489
                     /gene="phaC"
                     /locus_tag="VFU20_02515"
     CDS             <1..1489
                     /gene="phaC"
                     /locus_tag="VFU20_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HEU5285368.1"
                     /translation="SKAGARDRRFAAPEWRENPLFDTIRQTYLALSDRMLGSVDEIDG
                     LDEATRHKLKFAVRNFVDAMSPSNFALTNPQVLKRTIETRGENLLKGLANMLKDIAAG
                     QVTQSKPGAFEVGRDLATTPGKVIHETPLYQLIQYTPTTQSVLETPVVVFPPWINRFY
                     ILDLTPEKSFVKWCVDQGVSLFMVSWKSADESIADATLDDYVLKGQIDAIDTIRGLLG
                     VEGVHAIGYCVAGTTMAATLAYLAAKGESAKVKSATFFTAQVDFSEAGDLKLFLGDEV
                     MGLLQQLTTEKGYLDGRYMAATFNLLRGRDLIWNYVVNNYLLGDEPPPFDLLHWNSDT
                     TNLPAGWHRNYLDTLYRGNKLVESGGISVGGTSIDLGRVNTPCYIQAGREDHIAPPQS
                     VWKIMDHFTGDKRFVLAGSGHIAGVVNPPAAGKYQYWVNDEPSDTLEAFIAGATERQG
                     SWWPDWIEWLKKQDPKTVKAEGARIPGKGRLKAIEDAPGRYVKER"
     gene            complement(1496..1717)
                     /locus_tag="VFU20_02520"
     CDS             complement(1496..1717)
                     /locus_tag="VFU20_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285369.1"
                     /translation="MLNLASLIIGIVALVFAVVAFLPLLGWANWLIIPLAVIGAAVGM
                     ASSGTAGRNLNLFVIVVGVVRLMLGGGIL"
     gene            complement(1735..2157)
                     /locus_tag="VFU20_02525"
     CDS             complement(1735..2157)
                     /locus_tag="VFU20_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285370.1"
                     /translation="MRLLLLLSALALGSCAPVPAAAPQGPVGSMECGIVGSSGWAAWV
                     NAMPGPNARPTLIVTGKVTVPTGGYRFAWRDMRVMESYPVQVAVELDAIPPAGPATQA
                     LVTHDVRGQWPIDPPVGALTVRCGDMVLVRVAPVETAH"
     gene            complement(2194..3108)
                     /gene="rarD"
                     /locus_tag="VFU20_02530"
     CDS             complement(2194..3108)
                     /gene="rarD"
                     /locus_tag="VFU20_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HEU5285371.1"
                     /translation="MNAASLAQPNREIRRGFLFGVAAYGLWGVLPIYFKAIAAVAAVD
                     IVAHRVLWSLPFLALLLFLGRGWGEVVQAVRRRKTLVLLLCTAVLIATNWLLYVYAVT
                     SGHILAGSLGYYLNPLANVLLGRFILKERLSWLQWSAIAIAAAGILALIGGALDQLWI
                     SLTLAVTFATYGLLRKIALVDAVAGLAIETALLLPLAIAWLAWRLAAGEPSFGSTDGE
                     TGLLLLAGVVSTIPLLLFTGAARRLPYSTLGMLQFLAPTLQFVCAVWLYGEPFGRAHA
                     IAFGAIWTALALYMLALVRSPRAPQPPE"
     gene            3265..3915
                     /locus_tag="VFU20_02535"
     CDS             3265..3915
                     /locus_tag="VFU20_02535"
                     /inference="COORDINATES: protein motif:HMM:NF027558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4893 domain-containing protein"
                     /protein_id="HEU5285372.1"
                     /translation="MTALAGALAGCAGARPEEPRYRPAASVLAEPPLKSEAWMEIATA
                     ADESRLARIGLAWQSALADARRRHAAEIRREGALLEPRAALPRPAPTPGSYNCRLIKL
                     GRIGDKGLAFERFKPFFCYVQVEDDLLTIVKQTGSQRPAGRLWEDDRPDRLIFLGSLA
                     LGDEQQPLAYAEDPKRDMAGVLERIGPFRWRLVIPWPQGSSKLDVFELTPVADQPE"
     gene            3912..4313
                     /locus_tag="VFU20_02540"
     CDS             3912..4313
                     /locus_tag="VFU20_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU5285373.1"
                     /translation="MSTAGRLADRPTNAAGRLADPSEVRAHLVAAAKAGEPVTYSELL
                     NALGHEFSRPKMRALCKVLGAVDEEAAERGEPELAVLVVRQSDGLPGQGWWVAGGASC
                     HNYSGLWEGPEAKRLVAGLHARAFDYWRRRS"
     gene            4324..>5360
                     /locus_tag="VFU20_02545"
     CDS             4324..>5360
                     /locus_tag="VFU20_02545"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU5285374.1"
                     /translation="MAEPRSFTVTEDDDGIRLDRWFKRHLPEVSFNIVSRWARTGQLR
                     LDGRRATPGDRIEAGQQIRVPPPEAAPARVARPQRKAEPLAPEEAELVREMVIYSDPH
                     AFVLAKPPGLATQGGTKTHHHLDRLLDGLADEAGARPKLVHRLDKDTSGALLVARTAR
                     AAGHFAKAFSGRTARKVYWALVVGVPSAQEGLIDLPLAKQPGTGGEKMHVDKESGLAA
                     KTRWRLVDRAGNRAAWVELQPLTGRTHQLRAHMAAIGHPIVGDAKYGGAEAFLTGGIS
                     RKLHLHARRIRIDSPDGGAIDVTAELPPHFAESLATLGFEPMAGDMLPLDRPDPARSP
                     EIKQRRASAAA"
BASE COUNT          921 a         1889 c         1863 g          687 t
ORIGIN      
        1 gtccaaggcc ggggcgcgcg accggcgctt cgccgcgccc gaatggcgcg agaacccgtt
       61 gttcgacacc atccgccaga cctatctcgc tttgtccgac cggatgctcg gctcggtcga
      121 cgagatcgac gggctcgacg aggcgacccg gcacaagctc aagttcgcgg tccgcaactt
      181 cgtcgacgcg atgagcccgt ccaacttcgc gctgaccaac ccgcaggtct tgaaacgcac
      241 catcgagacc cgcggcgaga atttgctcaa gggcctcgcc aacatgctca aggacatcgc
      301 cgccgggcag gtgacccaga gcaagcccgg agcgttcgag gtcggccggg acctcgcgac
      361 cacgccgggc aaggtgatcc acgagacccc gctctaccaa ttgatccagt acaccccgac
      421 gacccagagc gtgctggaga ccccggtcgt ggtgttcccg ccgtggatca accgcttcta
      481 catcctcgac ctcacgcccg agaaaagctt cgtcaaatgg tgcgtcgacc agggcgtgtc
      541 gctgttcatg gtcagctgga agtcggccga cgagagcatc gccgacgcga ccctcgacga
      601 ttatgtcctg aaggggcaga tcgacgcgat cgacaccatc cgcggcctgc tcggggtcga
      661 gggagtccac gcgatcggct attgcgtcgc gggcacgacg atggcggcga ccctcgccta
      721 tctcgccgcc aagggggagt cggcgaaggt caagtcggcg accttcttca ccgcccaggt
      781 cgatttcagc gaagccggcg acctcaagct gttcctcggc gacgaggtga tggggctgct
      841 ccagcagctg accaccgaga aaggctatct cgacggccgc tacatggccg cgacattcaa
      901 cctgttgcgc ggccgcgacc tcatctggaa ctatgtcgtc aacaattacc tgttggggga
      961 cgagccgccg ccgttcgacc tgctgcactg gaacagcgac accaccaacc tgccggccgg
     1021 ctggcaccgc aactatctcg acacgctgta ccgcgggaac aagctcgtcg agagcggcgg
     1081 gatcagcgtc gggggcactt cgatcgacct cggccgggtg aacacgccct gctacatcca
     1141 ggccggccgc gaggaccata tcgcgccgcc gcagagcgtg tggaagatca tggaccactt
     1201 cacgggcgac aagcgcttcg tgctcgccgg ctcggggcat atcgccgggg tcgtcaaccc
     1261 gccggcggcc ggcaagtacc aatattgggt caacgacgag ccgagcgaca cgctcgaggc
     1321 gttcatcgcc ggcgcgaccg agcgccaagg cagctggtgg cccgactgga tcgagtggct
     1381 gaagaagcag gacccgaaga cggtcaaggc cgaaggagcg cgaatcccgg gcaagggccg
     1441 gctcaaggcg atcgaggacg cgcccggccg ctacgtcaag gagcgctagc ttccgctaca
     1501 ggatgccgcc gcccagcatc agccggacga ctccgacgac gatcacgaac aggttgaggt
     1561 tgcgcccggc ggtgccgctc gacgccattc cgaccgcggc gccgatcacg gccagcggga
     1621 tgatcagcca gttggcccag ccgagcaggg gcagaaaggc gaccaccgcg aagacgagcg
     1681 cgacgatgcc gatgatgagc gaggcgagat tgagcatggg cggccgctta gcagctaatg
     1741 cgcggtttcc acaggggcga cccgaaccag caccatgtcg ccgcagcgga ccgtcagcgc
     1801 gccgaccggc gggtcgatcg gccactggcc gcgcacgtcg tgggtaacca aagcctgggt
     1861 cgcgggcccg gccggcggga tcgcgtcgag ctcgaccgcg acctgcaccg gatagctttc
     1921 catcacccgc atgtcgcgcc aggcgaacct atagccgccg gtcggcacgg tgaccttgcc
     1981 ggtgacgatc agcgtcggcc gggcattcgg gcccggcatc gcgttgaccc acgccgccca
     2041 gccgctcgag ccgacgatcc cgcactccat cgaccccacc gggccctggg gagcggcggc
     2101 ggggaccggg gcgcacgagc cgagcgccaa ggcggacaat agcagcagca gtctcatcgt
     2161 ccccagcaat gcgggggcgg gctgcgaagt tccctattcg ggcggctggg gcgcgcgcgg
     2221 gctccgcacc agcgccagca tgtacagcgc cagcgccgtc cagatcgcac cgaaggcgat
     2281 cgcatgggcg cggccgaacg gctcgccgta gagccacacc gcgcacacga attgcagcgt
     2341 cggggcgagg aactggagca tcccgagcgt cgagtagggc aggcgccggg cggcgccggt
     2401 gaacagcagc agcgggatgg tcgaaaccac gcccgcgagc agcagcagtc ccgtctcgcc
     2461 atcggtcgag ccgaagctcg gctctccggc ggcgaggcgc caggcgagcc aggcgatcgc
     2521 cagcggcagc agcagcgcgg tctcgatcgc gaggcccgcg accgcgtcga ccagggcgat
     2581 cttgcgcagc agcccgtagg tggcgaaggt caccgccagg gtcaggctga tccacaattg
     2641 gtcgagcgcc ccgccgatca gggcaaggat ccccgccgcg gcgatggcga tcgcgctcca
     2701 ttgcagccac gacaggcgct ccttgaggat gaagcggccg agcagcacgt tggccagcgg
     2761 gttgaggtag tagccgaggc ttccggcgag gatgtggccg ctggtcaccg catagacgta
     2821 gagcagccag ttggtggcga tcagcaccgc ggtgcacagc aacaggacca gcgtcttgcg
     2881 gcggcggacg gcctggacga cctcgcccca gccgcggccc aggaacagca gcagcgccag
     2941 gaacggcagc gaccacagca cccggtgggc gacgatgtcg accgccgcga ccgcggcgat
     3001 cgccttgaaa tagatcggca gcacgcccca cagcccgtag gcggcgaccc cgaacaggaa
     3061 gccgcggcgg atttcccggt tgggctgggc caggctggcg gcgttcatcc gacgcgcgcc
     3121 tagcggcgct gagcgcgaag tgaaagctcg tgatgaacat gcggttggtt gaaaccgaaa
     3181 gcccggcctg tcacttgtaa gaaccgaact tgtcgaaagg tccgtgccct gcgtttgcgt
     3241 tcctcattgc ggcgcgctgc ctgtgtgacc gcccttgccg gcgcccttgc cggctgcgcg
     3301 ggcgccaggc ccgaggagcc ccggtaccgc ccggccgcat cggtgctggc cgagccgccg
     3361 ctgaagtccg aagcgtggat ggagatcgcc accgccgcgg acgaatcgcg gctcgcccga
     3421 atcggccttg cgtggcaatc ggcgctcgcc gacgcgcggc ggcgccatgc cgccgaaatc
     3481 cgcagggagg gcgcgctgct cgagccgcgg gcggcgctgc cgcggccggc gccgaccccc
     3541 gggagctaca actgccggct gatcaagctc ggcaggatcg gcgacaaggg cctggcgttc
     3601 gagcggttca agccgttctt ctgctacgtc caggtcgagg acgaccttct gaccatcgtc
     3661 aagcagaccg gcagccagcg gccggcgggg cgcctgtggg aagacgaccg gcccgaccgc
     3721 ctgatcttcc tcggcagcct ggcgctgggt gacgagcagc agccgctcgc ctacgccgag
     3781 gaccccaagc gcgacatggc cggagtgctc gagcgcatcg gcccgttccg ctggcggctg
     3841 gtgatcccgt ggccgcaggg cagttccaag ctcgacgtgt tcgagctgac cccggtggcc
     3901 gaccagcccg aatgagcacc gcggggcggc ttgccgaccg gccaaccaat gccgcggggc
     3961 ggctggccga ccccagcgag gtgcgcgcgc acctggtcgc cgccgcgaaa gccggcgaac
     4021 cggtcacgta ttcggagctt ttgaacgccc tcggccacga gttctcgcgg cccaagatgc
     4081 gcgccctgtg caaggtgctg ggcgcggtcg acgaggaggc ggcggagcgc ggcgagcccg
     4141 agctcgcggt gctggtggtg cgccagtcgg acgggctccc cggccagggc tggtgggtgg
     4201 cgggcggagc ctcctgccac aactacagcg gactgtggga agggccggag gcgaagcgcc
     4261 tggtcgccgg gctgcacgcg cgggcgttcg actattggag gcgcagaagc taatcgcccg
     4321 gccatggccg aaccccgcag cttcaccgtc accgaggacg acgacggaat ccgcctcgac
     4381 cgctggttca agcgccacct gcccgaggtc agcttcaaca tcgtctcgcg ctgggcgcgg
     4441 accggtcagc tgcgcctcga cggcaggcgc gcgacccccg gcgaccggat cgaggccggg
     4501 cagcagatcc gcgtcccgcc gcccgaagcc gccccggcgc gggtcgctcg gccgcagcgc
     4561 aaggccgagc cgctggctcc cgaggaagcc gagctggtgc gcgagatggt gatctattcc
     4621 gacccccacg ccttcgtcct cgccaagccg cccggcctcg cgacccaggg cggcaccaag
     4681 acccaccacc acctcgaccg gctgctcgac gggctcgccg acgaggccgg cgcccggccc
     4741 aagctggtcc accggctcga caaggacacg tcgggcgcgc tgctggtcgc tcgaacggcg
     4801 cgggcggcgg gccatttcgc caaggccttt tccggccgca ccgcgcgcaa ggtctattgg
     4861 gcgctggtcg tcggggtgcc gtcggcccag gaggggctga tcgacctgcc gctggccaag
     4921 cagcccggca ccggcggcga gaagatgcac gtcgacaagg aaagcgggct ggcggcgaag
     4981 acccgctggc ggctggtcga ccgcgccggc aaccgcgccg cctgggtcga attgcagccg
     5041 ctgaccggcc gcacccacca gctgcgcgcg catatggcgg cgatcggcca cccgatcgtc
     5101 ggcgacgcca aatacggcgg cgccgaagcc ttcctgaccg gcggcatcag ccgcaagctc
     5161 cacctccacg cgcggcggat ccggatcgac tcacccgacg gcggagcgat cgacgtcacc
     5221 gccgagcttc cgccgcattt cgccgagagc ctggcgaccc tgggcttcga gcccatggcc
     5281 ggcgacatgc tgccgctcga ccggccggac ccggcgcgga gcccggagat caagcagcga
     5341 cgggcctccg cggcggccaa
//
LOCUS       DASTZO010000094         2828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1077641,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000094 DASTZO010000000
VERSION     DASTZO010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2828
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077641"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..647
                     /locus_tag="VFU20_02550"
     CDS             <1..647
                     /locus_tag="VFU20_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285375.1"
                     /translation="WGAPVNAGPAINTAADEFCPTPARGNRFFFVRRLSATNSDIYVV
                     KHGPNGFGTPQPLPSGASAINSPWEEWSPSWFETEDGREILYFSSTRNGHPAQDIFYS
                     VNFGPAQFAPGGVNSSASDARPNVRHDGLEIVWDSTRTGSLGATDIWSSTRSTVDDPW
                     GTAVQLGNGINSSFGESRASLSWDGTTLVFGSTRPGEGSADIYVSTREKVTGSD"
     gene            complement(666..>2828)
                     /locus_tag="VFU20_02555"
     CDS             complement(666..>2828)
                     /locus_tag="VFU20_02555"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5285376.1"
                     /translation="ERGPAAAQAIVAQVQRLDPCNEEATRLALKIDHQTGDRGSLHRH
                     YELLSKRLREDYDAAPSRETQELFQRLSGNTTAATSPLARPPDPPPRVEPASAGPPGD
                     APGRGLARRALVASLAALAAVALLIFGWWSLRPQSANPDQPPLIAVLPFEQQPAGDAF
                     LAEGLWEDTRLALSQTQTLRVLGRATTLEMAKAGRSPKAYRDRLGVDYVLEGVVRRQG
                     DRVRVTTSLTRTRDGIGIWHSAFAGRLGDPLALQAAVAKGIEGRLRGQLARGGGKVPE
                     QIATTPEVYELYSKSRSLLRTRSPRAAQEAIQLLERALQLDRNFAPAWSMLGSANHFG
                     RFSPGASPEWRARARADVHRALELAPRLAEAHAVLALIDGLGSPVAVRSLERAVALDP
                     GNSDAWNWLGIARAQQGDTDGATQAYQKSIEIDPFLAPALSNLAGALLDRQDHQGFER
                     LLRRISRADGGATLVTALRAHRWFREGDYSRAMKPLLKLASRSGELDSFVVWKIEEGL
                     LRLGLVDQARALSGRPEQFVAELRGERLRGEFEGGRIPSPREYWVTVEIATVSSRAML
                     NLGRGRMLVDSYRQGFESRQDFLTTLNTYGEPVIVIPGLAIALQSSGQALEAEALLKA
                     IENDAEARVERHPGDADAVWQLARIRAAQGRENDAIDLLSRALDHGWLPDGRFYPLDI
                     ADDPPVRGLRGDPRFERLRRRALAHIARERAELGPVRI"
BASE COUNT          468 a          942 c          969 g          449 t
ORIGIN      
        1 gctggggcgc gccggtgaac gccgggccgg cgatcaacac ggcggctgac gaattctgcc
       61 cgacgccggc gcgcggcaac cgctttttct tcgtccggcg gctgagcgcc accaactccg
      121 acatctatgt cgtcaagcat gggccaaacg gcttcggaac gccacagccc ctgcccagcg
      181 gagcgagcgc catcaacagc ccgtgggaag aatggagccc ctcctggttc gagaccgagg
      241 acgggcggga aatcctctat ttctccagca cccggaacgg acacccagcg caggacatct
      301 tctacagcgt caatttcggc ccggcgcaat tcgccccggg gggcgtcaac agctccgcca
      361 gcgatgcccg gccgaacgtt cgccatgacg ggctcgagat cgtctgggac tcgacccgga
      421 ccgggagcct gggagcgacc gacatctggt cgtcgacccg ttcgaccgtc gacgatccct
      481 ggggcacggc ggtccagcta ggcaacggca tcaacagcag cttcggggag agccgcgcgt
      541 cgctgtcgtg ggacggcacg acgctggtgt tcggttcgac tcgtccgggc gaggggtcgg
      601 ccgatatcta tgtgtcgacc cgggagaagg tcaccggatc ggactgagcg ggagagcgcg
      661 ccgggctaga tccgaaccgg tcccagctcg gcgcgttcgc gggcgatgtg cgcgagggcc
      721 cggcggcgca gccgttcgaa gcgcgggtcg ccgcgaagcc cgcggacggg cgggtcgtcg
      781 gcgatgtcga gcggatagaa gcggccgtcc ggcagccagc catgatccag cgcccgtgac
      841 aggaggtcga tggcgtcgtt ttcacgcccc tgggcggcac ggatgcgcgc cagttgccaa
      901 acggcgtcag cgtcgccggg atgccgctcg acccttgcct cggcgtcgtt ttcgattgcc
      961 ttgaggagag cctcggcttc aagggcttgc ccggacgact gcaaggcaat cgccaggcct
     1021 ggtatgacga tgaccggctc gccataggtg ttgagcgtcg tcaggaagtc ctgtcgagat
     1081 tcaaacccct gccgatagga atcgacgagc atcctcccgc gcccgagatt gagcatagcg
     1141 cgactcgaca cggtggcgat ctcgacggtg acccaatatt ctcgcgggga ggggatccgg
     1201 cctccttcga actcaccacg aagccgttct ccacggagct ctgcaacgaa ctgctctggc
     1261 cggccggaca atgctcgagc ctggtcgaca agaccgagcc gcaacaatcc ctcttcgatc
     1321 ttccagacca cgaagctgtc gagttccccg gaacgcgagg ccagcttcag caacggtttc
     1381 atcgctcgtg aatagtcgcc ttcgcggaac cagcggtgag ctcgaagcgc cgtgaccagg
     1441 gtcgcgccgc cgtcagcccg ggaaatgcgc cggagcaggc gctcgaatcc ctgatgatcc
     1501 tgtcggtcga gcagcgcgcc ggccaggttg ctgagcgccg gagcgaggaa cgggtcgatc
     1561 tcgatcgatt tctgataggc ctgggttgca ccgtcggtgt cgccttgttg ggcgcgggcg
     1621 attccgagcc agttccaggc gtcgctattg ccggggtcga gggcgacggc ccgctcgagc
     1681 gaccgcacgg cgaccggcga gccaagtccg tcgatcagtg ccaagaccgc gtgcgcttcg
     1741 gcaagcctgg gcgcgagttc cagcgcgcga tggacatccg ccctggcccg ggcccgccat
     1801 tcagggctgg ccccggggct aaaccgaccg aaatgattgg ctgagcccag catcgaccag
     1861 gccggggcga aattccggtc cagctgcagc gcacgttcaa gaagttgaat ggcttcctgc
     1921 gcggcgcgcg ggctcctcgt gcgaagcagc gatctcgact tgctgtagag ctcatagact
     1981 tccggagtgg tggcgatctg ctcggggact ttgcctccgc cgcgcgccag ctggccgcga
     2041 agccgacctt cgattccctt ggcaaccgcc gcttgcaggg ccagcgggtc gccgagccgg
     2101 ccggcaaagg ccgaatgcca gatgccgatc ccgtcccttg tccgggtcag acttgtcgtc
     2161 actcgaaccc ggtcaccctg gcggcggacg actccctcga ggacataatc gacgccgagc
     2221 cggtcgcgat acgccttggg cgatcggccc gcttttgcca tctccagcgt ggttgcgcgg
     2281 ccgagcacgc gcaaagtctg ggtttgcgac agggcgaggc gcgtgtcttc ccacaggccc
     2341 tcggccagga atgcgtcgcc ggcgggctgc tgctcgaacg gcagcacggc aatgagcggc
     2401 ggctggtcgg ggttggccga ctggggccgc agagaccacc agccgaagat gagcaaagcg
     2461 acggcggcca gcgcggccag cgacgcaacc agcgctcgcc tcgccaagcc gcgaccgggc
     2521 gcatcgccag gcggcccggc actggccggc tcgacgcggg gcggcgggtc cggcgggcgg
     2581 gcgagcggcg aggttgccgc cgtcgtgttg cccgacagcc gttgaaatag ctcttgggtc
     2641 tcgcgggacg gggccgcgtc ataatcctcg cgcaagcgct tgctcagcaa ttcatagtgc
     2701 cggtgcagcg acccgcgatc gccggtttgg tgatcgatct tcagcgccag ccgagtcgcc
     2761 tcttcgttgc atggatcgag cctttgcacc tgggcaacga tggcctgggc agccgccggc
     2821 cctcgctc
//
LOCUS       DASTZO010000095        20848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_869055,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000095 DASTZO010000000
VERSION     DASTZO010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 20848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20848
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869055"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VFU20_02560"
     CDS             <1..823
                     /locus_tag="VFU20_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HEU5285377.1"
                     /translation="ATGIDARGRKQYRYHPDFRANADASKFAGLVDFGKALPKIRRTV
                     EKHLNQRKLSREAVLAAVVRLLDTEYLRVGNEEYAKENKSFGATTLLSRHVSETGRKL
                     KMRFAGKHGIVREVTITDRRLARVVRRCQELPGQALFQYVNGDGEPQQITSADVNDYI
                     REASGGDFTAKHFRTWGASVIAFEQLLEAGDKRKVGVGTMIEPVAEALGNTVAISRKS
                     YVHPALVEAAKERPRDPLQGMERPRRRKWLSSAEVGLLEFLKRQSRRRKTAAAPA"
     gene            844..1962
                     /locus_tag="VFU20_02565"
     CDS             844..1962
                     /locus_tag="VFU20_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel domain-containing
                     protein"
                     /protein_id="HEU5285378.1"
                     /translation="MTGAMRDPMQRWDGFLAWIQLNSEALLAGVLVALALVAGMLVLR
                     SAGERITASDPDYRSWKSVIGRVLAKTSLAFMIVAALAIVTNFVEPPRRVARIIDILF
                     IIAFAIQGAVWARELILGAIGRRIGEEPGETTLGNAASVIRVLVSIALFAIAFIVILD
                     NVGVNVTALVAGLGIGGIAIGLAAQGIFSDLFAALAILFDKPFKRGDVIRYDNSVGTV
                     ERIGLKTTRLRSLTGEQLVMANTKLLEREIHNLADGRSRRSVLTFGLVYQTPVDQLER
                     VPELARAAVEARKGCKFVRCTLSALSPSSLDHELLFEIASLDQDIIAGHRSAVILGLL
                     KVFAAEGIEFAYPTQTTFAAAPDGTLVMPYAPPAPAKS"
     gene            1962..2426
                     /locus_tag="VFU20_02570"
     CDS             1962..2426
                     /locus_tag="VFU20_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010174760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU5285379.1"
                     /translation="MPAASLDEIRGRVGTEVGVSDWLAIDQDRIDAFAEATEDKQFIH
                     VDAEAAARTPFGGTVAHGFLSLSMLSRMGAEAMLVPETLRMGINYGLDRVRFIAPVRS
                     GKRIRGRFVLDSVEDKAPGQILVRHSVTVEIEGEDKPALTAQWLALLFVQGD"
     gene            2428..3609
                     /locus_tag="VFU20_02575"
     CDS             2428..3609
                     /locus_tag="VFU20_02575"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5285380.1"
                     /translation="MPARDAVIVATARTPIGKAYRGAFNATPSPTLAAHPIRAVVERA
                     RLDPAEVDDVIIGAALQQGVQTTIGRTAALRAGLPVTVSGMSIDRQCASGLMAIATAA
                     KQVIVDRMDIVIAGGVESISLVQTGEMRLGPDPELLSMHNGVYMPMIDTAEIVGHRYS
                     VSRERCDEYALRSQQRTAAAQAEGRFADEIIPVTATMAVKDRETGVVSMREVTLDKDE
                     GNRPDTTLEGLAKLEPVRGPDKLITAGNASQLSDGASVCVVMEAGLAEKRGLEPLGRY
                     LGMAVAGTEPEEMGIGPVFAVPKLLDRFGLKVGDIGLWELNEAFAVQVLYCQDKLGIP
                     DEIFNVDGGAISIGHPYGMSGARLTGHALIEGKRRGARHVVVTMCVGGGMGAAGLFEV
                     L"
     gene            3616..4215
                     /locus_tag="VFU20_02580"
     CDS             3616..4215
                     /locus_tag="VFU20_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HEU5285381.1"
                     /translation="MDAVELIALASSVSLLAGWRLYLVTFATGLAMKMGWIDLPERLQ
                     ALDVLANDWVIGIAAAGAVAEFFADKVAWVDSAWDAVHSFIRPVGGALLSLAIIDAGD
                     PAWQVASFLLGGAAAFTAHAGKAGARTLVNASPEPFSNVVVSTAEDVATGGLLALAIA
                     NPVAAALIAVILVIASLWLVLAARRALKRLLEPKRRQAG"
     gene            4239..4511
                     /locus_tag="VFU20_02585"
     CDS             4239..4511
                     /locus_tag="VFU20_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285382.1"
                     /translation="MPVSEQPLVERIARVLAGAAHSSNAEGSDPSAGGKIDLVWREHV
                     NQALAVLHTMREPDAQMASAGDSETWARMVEAAIAEAEAAAEPSGV"
     gene            complement(4500..5732)
                     /gene="phaZ"
                     /locus_tag="VFU20_02590"
     CDS             complement(4500..5732)
                     /gene="phaZ"
                     /locus_tag="VFU20_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate depolymerase"
                     /protein_id="HEU5285383.1"
                     /translation="MLYDAYEVQRSFLAGASRLAGLGAGWLNNPANPLSYSSMGPVVA
                     ASLDVFAHASALRGKPEFGLGSTTIGRKTVPVREEIVVAKPFGDLKHFVRVGAPGDHP
                     KLLIVAPMSGHFATLLRGTVERLLPRHDVYITDWKDAKLVPLSDGSFDLDDYVDYLIE
                     FLEAIGEAGGERPHMLAVCQPAAPAFAATALMAADKHPWRPKSLTMMGGPIDTREAPT
                     AINTLATQRPHAWFENNVVATVPMIYPGAGRKVYPGFLQLAGFMTMNLGDHLISHWEM
                     FKHLVEGDEESADATRAFYEEYRSVSDMSAEFYLQTVDVVFQRHLLPDGQYLHRGRRV
                     DPAAIRDTALLAIEGERDDISGIGQTRAALDIARRLPAAKKKYHLARDVGHYGIFNGR
                     KWRENIAPVVEKWIASNA"
     gene            5873..7660
                     /locus_tag="VFU20_02595"
     CDS             5873..7660
                     /locus_tag="VFU20_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HEU5285384.1"
                     /translation="MAAKSEKQPEGTRKIGNLRLVWRYASKYPQQIAAALFFLVLSSA
                     ATLAIPYGFKRVIDQGFGSDGASAEAVGTAFQYLLMIVVVLAAATALRFYFVSWIGER
                     TIADLRVAVQRNLLTLPPRFFEENRPSEIASRLTADTAILEQVVGSSISIALRNLATG
                     LGGIVYLFALSPKLAGMLVIGIPVVFGPVIFFGRRIRSLSRASQDRIADVGSTVSETL
                     GAMKVVQAFGQEERELGRFRSAVENAFAVARRRFSMRAVMTFTIIALIFGSIVMLLWE
                     GAIDVAAGRMTGGSIAAFVFTGVLVGGAFGALTEVYGDLLRGSGAAGRIEELMREQAE
                     IRAPANPVPLPEPAQGALSFEKVSFLYPTRPDDKAVHDLSLAIAPGETVAVVGPSGAG
                     KSTLFQLALRFYDPQEGRVTLDGVDLRDADPAEIRKRIAFVPQESVLFAASARDNLRY
                     GRWDASDAEIQEAARAANAHEFIVQLPGGYDSFLGEGGARLSGGQRQRIAIARALLRD
                     SPLLLLDEATSALDAQSERLVQEALERLMRTRTTVVIAHRLATVRAAHRIVVMDSGRI
                     VEEGTHASLSAANGLYASLAKLQFAGQAA"
     gene            complement(7667..8365)
                     /locus_tag="VFU20_02600"
     CDS             complement(7667..8365)
                     /locus_tag="VFU20_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285385.1"
                     /translation="MAKSSTKKRSSKSSSSRSGKSTVDKIAKAAMSREMLGAGLAAAA
                     AAISASPAARRKIRDAGLDAADTASQAASTVMSSASKLGALIAEAVADAAQRVMSGKW
                     SDDEGETGAARGSASGARRAASSGARARKSTARKPAARKPAARKRATGARTAAAKSSG
                     GAARKAGTARKATGGVKSTARKTTTRKTTARKTTARKTTARKTTARRTTTRSGGRGSG
                     APSGGGGGAGGGGA"
     gene            complement(8682..9152)
                     /locus_tag="VFU20_02605"
     CDS             complement(8682..9152)
                     /locus_tag="VFU20_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285386.1"
                     /translation="MLRTFAAALIALTPAALAAQTAPVQPAVDFNTARVALGAWSYQS
                     APGGSMARFVDTTGTARLVLQCARATRRVTIALTSAAPAASLSVWTSNASRTVPARFE
                     PNAMRVAAELPAHDALLDGIAFSRGRIAVTMAGGVPLVAPAWPEAARTIEDCRI"
     gene            complement(9157..9678)
                     /gene="phaR"
                     /locus_tag="VFU20_02610"
     CDS             complement(9157..9678)
                     /gene="phaR"
                     /locus_tag="VFU20_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HEU5285387.1"
                     /translation="MAKSSGKATIKKYANRRLYDTESSTYITLDKLAEMIREGREFEV
                     VDAKTGEDITRHVLTQIIVDEESRGSTMLPVNFLRQLIGLYGNSMQGMVPQYLEAAMD
                     AFQRNQSVMRDAFGTNVLADLAKRNMALFGEASRAFTPKAQADAKKARDAELEKLRSE
                     LAELQAKVDRLGR"
     gene            9677..10762
                     /locus_tag="VFU20_02615"
     CDS             9677..10762
                     /locus_tag="VFU20_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5285388.1"
                     /translation="MNRSRDRHGHLLPEHSALQHERASQRAPRPLPLFLELVRMMADR
                     DPDLARAALDGLTAYEDAERPVPAAPRPEAARAGPVRLRDHGGSGAPAILIPSLINPP
                     HILDLDPEVSLASAIAGMGRHALLLDWGEARERSGLGVSGHVEQLLLPLLHELGEPAA
                     LVGYCLGGTMAIAAANLAPAERIVTLAAPWRFSAYPAESRANVQSMWRHSRGAAQALG
                     ALPMEVLQAAFWSLDPERTVSKFASFAALDPASAEARRFVQLEDWANQGEPLPCPAAA
                     ELIERFFGADLPGSGGWSIAGTTITDRIAVPALHFTADRDRITPAATAPAGDTVPIAA
                     GHVGMIVGRAREQLHAGLRRFLDPSCR"
     gene            10790..10866
                     /locus_tag="VFU20_02620"
     tRNA            10790..10866
                     /locus_tag="VFU20_02620"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10824..10826,aa:Arg,seq:ccg)
     gene            10913..12124
                     /locus_tag="VFU20_02625"
     CDS             10913..12124
                     /locus_tag="VFU20_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HEU5285389.1"
                     /translation="MAKILCVLYDDPVGGMPSSYPRDDLPKIERYPDGQTLPTPKAID
                     FRPGELLGCVSGELGLRRFLEDAGHTLVVTSDKDGPDSVFERELADADVVISQPFWPA
                     YLTAERIAKARNLKLAITAGIGSDHVDLAAAIDKGITVAEVTYCNSISVAEHVVMMIL
                     SLVRNYLPSHEWVLKGGWNIADCAARSYDVEGMHVGTVAAGRIGSAVLRRLKPFDMQL
                     HYFDRHRLPASVEQELGVAWHDSVEDMIAVCDVVTINCPLHPETENLFDAAMIGRMKR
                     GAYLVNTARGKIVDRDAVVAALASGQLAGYAGDVWFPQPAPRDHPWRTMPNHGMTPHI
                     SGTSLSAQARYAAGTREILECFFENRPIRDEYLIVEGGRLAGTGAHSYSPGDATGGSE
                     EAQRFKAETTA"
     gene            complement(12129..13181)
                     /locus_tag="VFU20_02630"
     CDS             complement(12129..13181)
                     /locus_tag="VFU20_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5285390.1"
                     /translation="MTGRRDPDGDRIFVHRVLIVIALAAVAFLLWQLRSLVLMLFGAV
                     VVASIFRALADVIRDRARLPDGIAVALSIGLILGSIAALVTVFGGQIAAQVHVLTEAL
                     PAAWRLLEERIAAMGFGEQLAALSESARSGGGGAASGLGKVVMSVGNGIADTLVVIFG
                     GIFLAAQPNFYRIGAIKLVPQSKRALVAEAMDDSERALRLWLRGQLFAMVVVGTLTGL
                     GLWLIGVPSALALGLLAGMLEFIPYAGPIAAAIPGVLLALVAGPETALWTVALYVGVQ
                     QFEGYVLQPLVQQYAVDLPPVVLLFSLLGFGMLFGIIGIILAAPLTVVSYVLVKRLYV
                     QEALGTKTPIPGEDKK"
     gene            complement(13178..14320)
                     /locus_tag="VFU20_02635"
     CDS             complement(13178..14320)
                     /locus_tag="VFU20_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEU5285391.1"
                     /translation="MIDILVPDEQEGTKAVVRAWLKQVGDSVAENDPLVELETDKVTQ
                     EVPAPAAGVLAEIVLDTDAEAVPGALLGRIDPKESVRPEPVEGRSAPAAEQGPSTSLG
                     TNEEGETRLSPSVRRALQQHGIDPSRIKGSGRGGRITREDVERALEGAAPSGADIPHD
                     RMRLKIAENMVRAVAQAPHVTAVFEADFSAVAAHKERQAGRGVKLSYTAYIVKAAAEA
                     MAVAPAINGRWEQDRIAVSPTIDIGVGTALGEKGLVVPVVRDAGNLGLEQIGLALDDL
                     TRRAREGKLTGGDVSGGSFTISNHGVSGSLLAAPIILHQGQAAILGIGKLEKRVAVRE
                     VDGDDAIVILPMAYVTLTIDHRVVDGHQTNAWLTRFVEIIEGWPAP"
     gene            complement(14322..16364)
                     /locus_tag="VFU20_02640"
     CDS             complement(14322..16364)
                     /locus_tag="VFU20_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5285392.1"
                     /translation="MAERDARRVAAEVDWREVARLVLTSREMDRVEEQELVPNKKILY
                     QFSARGHDLAQVLLGLQLRDGDAACGYYRSRPLLLALGVPLADALGSGMGLAGGYSDG
                     RDIGVVFNYPNPGGAHALPMSGGVGAQYTPAAGWAQAIAYKAEVLGQGPEDAIALVLG
                     GDASCATGGFWSALTIATTQQLPLLMYVEDNGYGISVPSEYQTPGKDIAANLASFSGL
                     TIFNGDGTDPAEAARLIADAVRHVRTQRAPALLRLTMPRLEGHSFQDTQSYKSEAEIK
                     AEWARDPLPKLKAFAAKLDWPALEADAAANVAAAVAEAEARGVSSPERVTDNVFFESE
                     LQQVGGQWNHGFTPPPSSDEPRPDGQRINMVTAIRRTLEQELEANPRVLLFGEDIGPK
                     GGVHAVTLGLQEKFGRARVFDTSLNEEGIVGRAVGMALAGLMPVPEIQFRKYAEPATE
                     QINDCGTMRWRTHNRFAAPLVLRVPGGFFKCGDPWHSQTNEVQFVHNPGWLVAVPSNA
                     EDAVGLLRGALRGNDPVLFFEHRAMLDDAWARRPWPGDDYVLPFGRAKKTREGEQITI
                     VTWGAMVPRCEAAADGVSADVIDLRTLMPWDRDTVLDSVARTRRCLVVHEDLRTGGFG
                     AEIAAVVADEAFLDLDAPVARVTMPDIPSPHHPTLMEAALPSVTRIRAEIDRLVGF"
     gene            complement(16357..17571)
                     /locus_tag="VFU20_02645"
     CDS             complement(16357..17571)
                     /locus_tag="VFU20_02645"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HEU5285393.1"
                     /translation="MLTETHAKAPNSARGGLADLPPVTSDSLLALIALANADPRPDKI
                     DVGVGVFRDAAGETPILKVMKKAERILLETQPTKAYLGSAGDTRFAELLRPILLGSHA
                     DDERIAGVQTPGGCGALRLGFELVATANPDARVFVSQPTWPNHPPIIRGARLQMIAYP
                     YYDRASGTVRFEDMLETLGTAREGDVVLLHGCCHNPTGADLNDEQWAKVARLIAERGL
                     LPFIDIAYQGFGRGLDEDSGGLRGILAACDDVLVAQSCDKNFSVYRDRVGSLFVKTGS
                     AEATARAMAHVFQRAREMWSMPPDHGAAAVRIILDEPELRAQWHGELDAMRGRINGVR
                     RRIAAADPRLAFIGTQFGMFSMLPLSLEQVLELRERHAIYMADSGRFNVVGMADHSID
                     RFVAAVVDALDG"
     gene            complement(17609..18682)
                     /gene="paaE"
                     /locus_tag="VFU20_02650"
     CDS             complement(17609..18682)
                     /gene="paaE"
                     /locus_tag="VFU20_02650"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HEU5285394.1"
                     /translation="MAEHFHALRVAEVVPETSEANSIRFEIPPELREKFAFRAGQHLT
                     LRAEIDGEEVRRNYSLCTAPDEQDWMVTVKRIAGGIFSNWVGDHLKAGDVIDVMPPHG
                     SFTVEFDPAKQRRYVGFAGGSGVTPVISLIRTALKKEPGSRFTLFYGNRDSASIIFLD
                     ALADLKDRYLGRFELYHFLAEEEGEVELFNGMLDKATCGDAVEHLVGEPESVDAWFIC
                     GPGPMMDAAEAALLERGVAKDRIHIERFTADRPPEAVAREIADLQTRAAGASVAVTLD
                     GRTRKVEFTQGNILDSARAAGLPAPFACKAGVCATCRAKVTSGKVEMAARYGLTDEEV
                     AAGYVLTCQSVPVGEGVAVDYDA"
     gene            complement(18684..19493)
                     /gene="paaC"
                     /locus_tag="VFU20_02655"
     CDS             complement(18684..19493)
                     /gene="paaC"
                     /locus_tag="VFU20_02655"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018250992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HEU5285395.1"
                     /translation="MASLPPIDTPRSATGAGAFDAPAAGEPDPARFDYLLRLGDDALI
                     LGQRLGEWCGHAPALEVDLSLANLALDLIGQATHLLGAAGDADRLAFHRDVLDFRNCL
                     LVEQPNGDFGRTIARQLLFSTYQHLLYQRLAESSDAFLRDIAAKAVKEVAYHRELVSE
                     WTVRLGDGTEESARRMSEGLEWCWRFIPELFEVDELLGGLVERGIAADPRAFEDEYRS
                     ALAAVLADAKLEAPAEQRPILGGRRGHHSEHLGHILAVMQFLPRTYPDATW"
     gene            complement(19599..19880)
                     /gene="paaB"
                     /locus_tag="VFU20_02660"
     CDS             complement(19599..19880)
                     /gene="paaB"
                     /locus_tag="VFU20_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003857353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HEU5285396.1"
                     /translation="MSDWPLWEVFVRSKGGLSHRHVGSVHAPDAELALRHARDTYTRR
                     QEGVSLWVVPSAEIVASDPDEAGPLFEPAADKIYRHPTFYDIPDEVKHI"
     gene            complement(19877..20845)
                     /gene="paaA"
                     /locus_tag="VFU20_02665"
     CDS             complement(19877..20845)
                     /gene="paaA"
                     /locus_tag="VFU20_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HEU5285397.1"
                     /translation="MASLDEHRDEAAQARFDELIAQDSRIEPRDWMPDAYRRTMIRQI
                     AQHAHSEIIGMQPEGSWITRAPSLRRKAILLAKVQDEAGHGLYLYCAAETLGTSREQM
                     IEALHSGKAKYSTIFNYPTLTWADIGAIGWLVDGAAIMNQVPLQRTSYGPYARAMVRV
                     CKEESFHQRQGYEIMIAMANGTAEQKRMAQDALNRWWWPSLMMFGPPDDNSPNSAQSI
                     RWRIKRETNDELRQKFVDITVPQADFLDLKVPDPDLRWNEGKNDGRGGYDFGEVDWDE
                     FYAVVRGEGPVAKERMAARRAAWDDGAWVREAAAAHEAKRRERKAA"
BASE COUNT         3173 a         7363 c         6995 g         3317 t
ORIGIN      
        1 ggcgaccgga atcgacgccc gcggccgcaa gcaatatcgc taccaccccg acttccgcgc
       61 caatgccgac gccagcaagt tcgccggcct ggtcgacttc ggcaaggcgc tgcccaaaat
      121 ccgccggacg gtcgaaaagc acctcaacca gcgcaagctc agtcgcgagg cggtgctggc
      181 ggcggtcgtg cggctgctcg acaccgagta tctgcgggtc ggcaacgaag aatatgcgaa
      241 ggagaacaag agcttcggcg ccacgaccct gctcagcagg cacgtcagcg agaccggccg
      301 caagttgaag atgcgcttcg ccggcaagca cgggatcgtc cgcgaggtga ccatcaccga
      361 ccgccggctc gcccgggtgg tccgccgctg ccaggagctg ccgggccagg ccctgttcca
      421 atatgtgaac ggcgacggcg agccgcagca aatcacttcg gccgacgtca acgactatat
      481 ccgcgaggcg agcggcggcg atttcaccgc caagcatttc cgcacctggg gcgcgagcgt
      541 gatcgcgttc gagcaattgc tcgaggcggg cgacaaaagg aaggtcggcg tcggaacgat
      601 gatcgaaccg gtcgccgagg cgctcggcaa caccgtcgcg atcagccgca agtcctatgt
      661 ccatccggcg ctggtcgagg cagccaagga gcggccgcgc gacccgctgc aagggatgga
      721 gcggccccgc cggcgcaaat ggctgtcgtc ggccgaggtc ggcctgctcg agttcctcaa
      781 gcggcagtcg cggcggcgca agacggcggc ggccccggct tgatcgggcc gcggcgtgcc
      841 gccatgacgg gcgccatgcg cgacccgatg cagcgatggg acggcttcct ggcctggatc
      901 cagctcaaca gcgaggcact gctggccggg gtcctggttg ccctggcgct ggtcgccggg
      961 atgctggtgc tgcgcagcgc cggcgagcgg atcaccgcgt ccgaccccga ctatcgaagc
     1021 tggaaatcgg tcatcggccg ggtgctggcc aagaccagcc tcgccttcat gatcgtcgcc
     1081 gcgctggcga tcgtcaccaa cttcgtcgag ccgccgaggc gcgtcgcccg gatcatcgac
     1141 atattgttca tcatcgcctt cgcgatccag ggcgcggtct gggcccgcga gctgatcctc
     1201 ggggcgatcg ggcggcggat cggcgaggag ccgggcgaaa ccacgctcgg caatgcggcc
     1261 tcggtgatcc gggtactggt cagcatcgct ctgttcgcga tcgccttcat cgtcatcctc
     1321 gacaatgtcg gggtcaacgt caccgcgctg gtcgctggcc tcggcatcgg cggcatcgcc
     1381 atcggccttg ccgcccaggg catcttctcg gacctgttcg cggcgctcgc gatcctgttc
     1441 gacaagccgt tcaagcgcgg cgacgtcatc cgctacgaca acagcgtcgg caccgtcgag
     1501 cgcatcgggc tcaagaccac ccggctgcgc tcgctgaccg gcgagcaatt ggtaatggcc
     1561 aacaccaagc tgctcgagcg cgagatccac aacctcgccg acggccgcag ccgccgctcg
     1621 gtcctgacct tcggcctcgt ctaccagacc ccggtcgacc agctcgagcg ggtgcccgag
     1681 ctggcgcggg cggcggtcga ggcgcgcaag ggctgcaagt tcgtccgctg cacgctgagc
     1741 gccctttcgc cctcgagcct cgatcatgag cttttgttcg agatcgccag cctcgaccag
     1801 gacatcatcg ccggccaccg ctcggcggtc atcctgggcc tgctgaaggt cttcgccgcc
     1861 gaaggcatcg aatttgccta cccgacccag accaccttcg ccgccgcgcc cgacggcact
     1921 ctggtcatgc cctacgcgcc gcccgctccg gcgaaaagct gatgccggcc gcgtcgctgg
     1981 acgaaatccg cggccgcgtc ggcaccgaag tcggagtttc ggactggctg gccatcgacc
     2041 aggaccggat cgacgcgttc gccgaggcga ccgaggacaa gcagttcatc catgtcgatg
     2101 ccgaggccgc cgcccgcacc cccttcggcg gcaccgtcgc ccacggcttc ctgtccttgt
     2161 cgatgctgtc gcggatgggc gccgaggcga tgctcgtgcc cgagaccctg cggatgggca
     2221 tcaattacgg gctcgaccgg gtgcgcttca tcgccccggt gcggtcggga aagcgcatcc
     2281 gcggccgctt cgtgcttgac tcggtcgagg acaaggcgcc ggggcagatc ctcgtgcgcc
     2341 attcggtgac ggtcgagatc gagggcgagg acaagcccgc gctgaccgcc caatggctcg
     2401 ccttattgtt cgtccaagga gattgagatg cccgcccgcg acgccgtcat cgtagcgacc
     2461 gcccgaacgc cgatcggaaa agcgtaccgc ggggcgttca acgcgacccc gtcgccgacg
     2521 ctcgccgccc acccgatccg cgcggtcgtc gagcgcgccc ggctcgaccc ggccgaggtc
     2581 gacgacgtga tcatcggcgc cgccctgcag cagggagtcc agaccaccat cggccggacc
     2641 gccgctttgc gcgccgggct tccggtgacc gtctcgggca tgtcgatcga ccgccagtgc
     2701 gcgtcggggc tgatggcgat cgccaccgcc gccaagcagg tgatcgtcga ccgcatggat
     2761 atcgtcatcg ccggcggcgt cgaatcgatc agcctggtgc agacgggcga gatgcgcttg
     2821 gggccggatc cggagctctt gtcgatgcac aacggcgtct acatgccgat gatcgacacc
     2881 gccgagatcg tcggccatcg ctattccgtc agccgcgagc gctgcgacga atatgcgctg
     2941 cgctcgcagc agcgcaccgc cgccgcccag gccgaagggc gcttcgccga cgagatcatc
     3001 cccgtcaccg cgaccatggc ggtcaaggac agggagaccg gcgtggtgtc gatgcgcgag
     3061 gtgactctcg acaaggacga gggaaaccgc cccgacacga ccctcgaggg cttggccaaa
     3121 ctggagccgg tgcgcggccc cgacaaattg atcaccgccg gcaacgccag ccagctgtcc
     3181 gacggagcgt cggtctgcgt cgtgatggag gcggggctcg ccgagaagcg cgggctcgag
     3241 ccgctcggcc gctacctcgg aatggcggtc gccggcaccg agcccgagga aatgggcatc
     3301 ggcccggtgt tcgcggtgcc gaaactgctc gaccgcttcg gcctcaaggt cggcgacatc
     3361 ggcctgtggg agctgaacga ggcgttcgcg gtccaggtgc tttactgcca ggacaagctc
     3421 gggatccccg acgagatctt caacgtcgac ggcggagcga tttcgatcgg ccacccgtac
     3481 ggaatgagcg gcgcccgcct gaccggccac gccttgatcg aaggcaagcg ccgcggcgcc
     3541 aggcatgtcg tcgtcaccat gtgcgtcggt ggcggaatgg gcgcggcggg gctgttcgag
     3601 gtcctgtaag cgaaaatgga cgccgtcgag ctgatcgccc tcgcctcgtc cgtcagcctg
     3661 ctcgccggct ggcggctcta cctggtcacc ttcgccaccg ggctggcgat gaagatgggc
     3721 tggatcgacc tgcccgagcg gctgcaggcg ctcgacgtgc tcgccaacga ttgggtgatc
     3781 gggatcgccg ctgcgggggc ggtggccgag ttcttcgccg acaaggtcgc ctgggtcgac
     3841 agcgcctggg acgcggtcca ctcgttcatc cgcccggtcg gcggggcctt gctgagcctg
     3901 gcgatcatcg acgccggcga ccccgcctgg caggtcgcga gcttcctcct cggcggcgcc
     3961 gcggcgttca ccgcccatgc cggcaaggcc ggcgcccgga cgctcgtcaa cgccagcccc
     4021 gagccctttt ccaacgtcgt cgtctcgacc gcggaggacg tcgccaccgg cggcctgctc
     4081 gcgctggcga tcgccaaccc ggtcgccgcc gccctgatcg cggtgatcct ggtcatcgcc
     4141 tcgctgtggc tggtgctcgc cgcccggcgc gcgctcaaac gcctacttga acccaaacgc
     4201 cgccaggcgg gttgagcctc caacaggagg accttgcgat gcccgtttcc gaacagccgc
     4261 tggtcgagcg cattgcccgg gtgctcgccg gcgccgccca cagcagcaac gccgagggca
     4321 gcgacccgtc ggccggcggc aagatcgacc tggtctggcg cgagcacgtc aaccaggcgc
     4381 tggcggtgct ccacaccatg cgcgagcccg atgcgcagat ggcgtcggcc ggcgacagcg
     4441 aaacctgggc gaggatggtc gaggcggcga tcgccgaggc cgaagccgcg gccgagccgt
     4501 caggcgtttg aggcgatcca cttctcgacg acgggagcga tattctcgcg ccacttgcgg
     4561 ccgttgaaga tgccgtaatg gccgacgtcg cgggccaaat gatatttctt cttcgccgcc
     4621 ggcagccgcc tggcgatgtc gagcgcggcg cgggtctggc cgatcccgga aatgtcgtcg
     4681 cgctcgccct cgatcgccag cagggcggtg tcgcggatcg ccgccgggtc gacccgccgg
     4741 ccgcggtgca aatattggcc gtccggcagc aaatggcgct ggaacacgac gtcgaccgtc
     4801 tgcaggtaga attcggcgct catgtcgctg accgagcgat attcctcgta gaacgcccgc
     4861 gtcgcgtcgg cgctctcctc gtcgccctcg accaggtgct tgaacatctc ccagtggctg
     4921 atcagatgat cgcccaagtt catggtcatg aacccggcga gctgcaggaa gcccgggtaa
     4981 accttccgcc cggcgcccgg gtagatcatc ggcacggtcg cgacgacatt gttctcgaac
     5041 caggcgtgcg gccgctgcgt cgccagcgtg ttgatcgcgg tcggcgcttc gcgggtgtcg
     5101 atcggcccgc ccatcatcgt caggctcttg ggccgccaag ggtgcttgtc cgccgccatc
     5161 agagcggtcg cggcgaacgc cggcgcggcc ggctggcaga ccgcgagcat gtgcggccgc
     5221 tcgccgcccg cctcgccgat cgcttccagg aactcgatca gataatcgac ataatcgtcg
     5281 aggtcgaagc tcccgtccga cagcggcacc agcttcgcgt ccttccagtc ggtgatatag
     5341 acgtcgtggc gcggcagcag ccgctcgacc gtgccgcgca gcagggtcgc gaagtggccc
     5401 gacatcggag cgacgatcag cagcttcgga tgatccccgg gagcgccgac ccggacgaaa
     5461 tgcttgaggt cgccgaacgg cttggcgacg acgatctcct cgcggaccgg aaccgtcttg
     5521 cgcccgatgg tcgtcgagcc caggccgaac tccggcttgc cccgcagcgc cgaagcatgg
     5581 gcgaacacgt cgaggctggc cgccacgacc gggcccatcg agctgtagga cagcgggttt
     5641 gcggggttgt tgagccatcc ggcgccgagc ccggcgagcc tgctcgctcc ggcaaggaat
     5701 gagcgctgca cttcgtaggc gtcgtaaagc atcagacctc ggggggaaaa gtgggcaagg
     5761 gactgaacag atgtggcggc cattgggtcc cgaagcaagg ggcgatgctg cattgcggaa
     5821 gctgcgccgc ggcaattgag gcccggccgc cacactgcta gtcgtcgcgg cgatggcggc
     5881 gaaatccgaa aaacagcccg agggcacgcg caagatcggc aatctgcggc tggtctggcg
     5941 ctacgcgtcg aaatatccgc agcagatcgc cgccgcgctg ttcttcctcg tcctgtcgtc
     6001 ggcggccacg ctcgcgatcc cctacggctt caagcgggtg atcgaccagg gcttcggctc
     6061 cgacggggcg agcgccgagg cggtcggcac cgcgttccag tatctgctga tgatcgtcgt
     6121 cgtgctcgcc gccgcgaccg ccctgcgctt ctatttcgtg tcgtggatcg gcgagcgcac
     6181 catcgccgac ctgcgcgtcg cggtgcagag gaacctgctg accctgccgc cgcgcttctt
     6241 cgaggagaac cgcccgtcgg agatcgcctc gcggctcacc gccgacaccg cgatcctcga
     6301 gcaggtggtc ggctcgagca tctcgatcgc tttgcgcaac ctcgccaccg ggcttggcgg
     6361 aatcgtctat ctgttcgctt tgtcgcccaa gctcgccggg atgctggtga tcggaatccc
     6421 ggtcgtgttc gggccggtga tctttttcgg ccgccggatc cggagcctca gccgcgcctc
     6481 gcaggaccgg atcgccgacg tcggctcgac ggtgtcggag accctggggg cgatgaaggt
     6541 tgtccaggcg ttcggccagg aggagcgcga gctcggtcgc ttccggtccg cggtcgagaa
     6601 tgcgtttgcc gtcgcccgcc gccgcttttc gatgcgcgcg gtgatgacct tcaccatcat
     6661 tgcgttgatc ttcggctcga tcgtcatgct gctgtgggaa ggcgcgatcg acgtcgccgc
     6721 ggggcggatg accggcggct cgatcgccgc cttcgtgttc accggggtgc tggtcggcgg
     6781 cgcgttcgga gcgctgaccg aggtctatgg cgacctgctg cgcggctcgg gcgccgccgg
     6841 gcggatcgag gagctgatgc gcgagcaagc ggaaatccgc gcgcccgcca accccgtgcc
     6901 gctgcccgag ccggcgcagg gcgcgctcag cttcgagaag gtcagcttcc tctatccgac
     6961 ccgccccgac gacaaggcgg tgcacgactt gagcctggcg atcgcacctg gcgaaacggt
     7021 cgcggtggtc ggcccgtcgg gtgccggcaa gtcgaccctg ttccagctcg cgctgcgctt
     7081 ctacgacccg caggaaggcc gcgtgaccct cgacggagtc gatctgcgcg acgccgatcc
     7141 cgctgaaatc cgcaagcgca tcgccttcgt cccgcaggag agcgtgctgt tcgccgcgtc
     7201 ggcgcgcgac aatttgcgct acggccgctg ggacgcgagc gacgcggaga tccaggaagc
     7261 cgcgcgggcc gccaacgcgc acgagttcat cgtccagttg cccggcggct acgacagctt
     7321 cctcggcgag ggcggcgccc ggctgtccgg cggccagcgc cagcgcatcg ccatcgcccg
     7381 agcgctgctg cgcgattcac cgttgctgct gctcgacgag gcgacgtccg cgctcgacgc
     7441 gcaaagcgag cggctggttc aggaagcgct cgagcggctg atgcggacgc ggacgaccgt
     7501 cgtcatcgcg caccggctgg cgaccgtccg cgccgcccac cggatcgtcg tcatggacag
     7561 cggccggatc gtcgaggaag ggacgcacgc ctcgctgtcg gccgccaacg gcctctacgc
     7621 gagcctcgcc aagctgcagt tcgccggcca ggccgcctag gcccccttag gctccgccgc
     7681 ctcccgctcc accgccgccg ccgctcgggg caccgctgcc gcgcccaccg gaacgggtgg
     7741 tagtgcggcg ggccgtggtc ttgcgcgcgg tggtcttgcg ggccgtggtc ttgcgggcgg
     7801 tcgtcttccg cgtggtggtc ttgcgcgcgg tactcttcac tccgccggtc gccttgcgcg
     7861 ccgtgccggc cttgcgcgct gcgccgccgc tgcttttcgc cgcggccgtg cgggccccgg
     7921 tggcccgctt gcgcgccgcc ggtttgcgcg ccgccggttt gcgcgccgtc gatttgcgag
     7981 cgcgagcacc gctgctcgcc gcgcggcggg cgcccgatgc ggagccgcgg gctgcgccgg
     8041 tctcgccttc atcgtccgac catttgccgc tcatcacgcg ctgcgccgcg tcggcgacgg
     8101 cttcggcgat cagcgcgcca agcttgctgg cgctcgacat caccgtgctc gccgcttgcg
     8161 atgcggtgtc cgcggcatcg agtccggcat cgcgaatctt gcggcgcgcg gccgggctgg
     8221 cgctgatcgc ggcggccgcg gcggccaatc cggcgccgag catctcgcgg ctcatcgccg
     8281 ccttggcgat cttgtcgacc gtcgatttgc cggaacgcga cgacgatgat ttggacgaac
     8341 gttttttcgt gctcgatttc gccatttgca aaacctccct tcggatggag gaaagacact
     8401 tcaggcgcgt aacagttccg tcgcggcggc gcggcgatgt ttcaacgcgc gacaaatgcg
     8461 gacgcgtaaa aggggaccgc cggcaaagcg gcgatcccct tcgacatggt cagcggccgg
     8521 aggttccagc cgttcgggtg cgcttaaagc atcacctcgc gaacgatttg gaccgacccc
     8581 tctgctgaac catgagacat cggatcacct cctttcgctg cgttgaagcc aggaacgaac
     8641 atgagtcttc atcgccgaca acgcaagtct tgtattgccg ttcaaattcg gcaatcctcg
     8701 atcgttcgtg ctgcttcggg ccacgccggc gcgaccagcg gcaccccacc cgccatcgtc
     8761 accgcaatgc gcccgcggct gaacgcgatt ccatcgagca atgcatcgtg cgccggaagc
     8821 tcggcggcaa cgcgcatcgc attcggctcg aagcgcgccg gcacggtgcg cgacgcattg
     8881 ctcgtccaca ccgacaggct cgcggccggc gccgcgctgg tcagcgcgat cgtcactcgc
     8941 cgcgtcgccc gcgcgcactg gaggaccagc cgagcggtgc cggtcgtgtc gacgaaccga
     9001 gccatgctgc cgcccggcgc ggattgatat gaccaggccc cgagcgcgac gcgggcggtg
     9061 ttgaaatcaa ccgcgggctg gaccggagcg gtctgggcgg cgagagcggc gggagtaagc
     9121 gcgatcagcg ccgcggcaaa cgttcgaagc atcgcgtcag cggcccagcc gatcgacctt
     9181 ggcctgcagc tcggcaagct cggagcgaag cttctcgagc tcggcgtcgc gagctttctt
     9241 cgcgtccgcc tgggccttgg gggtgaacgc ccggctcgcc tcgccgaaca gcgccatgtt
     9301 gcgcttggcg aggtcggcga ggacattggt tccgaacgcg tcgcgcatca ccgactggtt
     9361 gcgctggaat gcgtccatcg ccgcctcgag atattgcggc accatgccct gcatcgaatt
     9421 gccgtagagg ccgatcagct gtcgcaggaa attcaccggc agcatggtcg aaccgcgcga
     9481 ttcctcgtcg acgatgatct gggtcaggac atggcgggtg atatcttcac cggtcttggc
     9541 gtcgaccacc tcgaactcgc ggccctcgcg aatcatctcg gccagcttgt cgagcgtgat
     9601 gtaagtcgag ctttcggtgt catatagccg ccggttcgcg tacttcttga ttgtcgcctt
     9661 gcccgaagac ttcgccatga accgctcccg agaccgtcac ggccacctac taccggagca
     9721 tagtgcactg caacatgaac gggcctcgca aagggcaccg cgtccgctgc cgctgtttct
     9781 cgagctggtg cggatgatgg cggaccgcga cccggacctc gcccgcgccg cgctcgacgg
     9841 attgaccgcc tacgaggatg ccgagcggcc ggtgcccgcc gctccgcggc ccgaagccgc
     9901 tcgcgccggc ccggtgcgcc tgcgcgatca cggcggatcg ggcgcgcctg cgatcctgat
     9961 cccgtcgctg atcaacccgc cgcacatcct cgacctcgac cccgaggtgt cgctggcgtc
    10021 cgcgatcgcc ggcatgggcc gccacgcatt gctgctcgac tggggcgagg cgcgcgagcg
    10081 ttcgggcctc ggcgtctctg gccatgtcga gcagcttctg ttgccgctgc tgcacgagct
    10141 cggcgagccg gccgcgctgg tcggctattg cctcggcggg acgatggcga tcgccgccgc
    10201 caacctcgcg cccgccgagc gcatcgtcac cctcgccgcc ccgtggcgtt tttccgccta
    10261 cccagcggaa agccgagcga atgtgcagag catgtggcgc cattcgcgcg gcgccgcgca
    10321 agcgctcggt gcgctgccga tggaagtgct ccaggcggcg ttctggtcgc tcgaccccga
    10381 gcggacggtg agcaagttcg ccagcttcgc cgcgctcgac cccgcaagcg cggaggcgcg
    10441 ccgcttcgtc cagctcgagg attgggcgaa ccagggcgag ccgctgccct gcccggccgc
    10501 ggccgagctg atcgagcgat tcttcggcgc cgacctgccc ggaagcggcg gctggagcat
    10561 cgccggaacg acgatcaccg accggatcgc cgttcccgcg ctccacttca ccgccgaccg
    10621 cgaccgaatc actcccgccg cgaccgcgcc cgccggcgac accgtgccga tcgccgcggg
    10681 ccacgtcggc atgatcgtcg gccgggcgcg cgagcagctc cacgccggcc tccggcgctt
    10741 tctcgaccct tcttgccgct gagcggtcac gaggctaagc ggcccccacg cacccatagc
    10801 tcagctggat agagcgctgc cctccgaagg cagaggtcag aggttcgaat cctcttgggt
    10861 gcgccatatt ctgtgccgcg ttcgatgctc ctgacctgga aggaagctcg caatggccaa
    10921 gatcctgtgc gttctctacg acgatcccgt cggcgggatg ccctcctcct acccgcgcga
    10981 cgatttgccg aagatcgagc gctatcccga cggccagacc ctgccgactc ccaaggcgat
    11041 cgattttagg cccggcgagc tgttgggctg cgtgtcgggc gagctcggcc tgcgccgatt
    11101 tctggaagac gccggccaca ccttggtcgt cacctcggac aaggacggcc ccgacagcgt
    11161 cttcgagcgc gagctggccg acgccgacgt cgtcatctcg cagcccttct ggcccgccta
    11221 tctgacggcc gagcggatcg ccaaggcgcg caacctcaag ctcgcgatca ccgccgggat
    11281 cggctcggac catgtcgacc tggccgcggc gatcgacaag ggcatcaccg tcgccgaggt
    11341 cacctactgc aactcgatca gcgtcgccga gcatgtggtg atgatgatcc tcagcctggt
    11401 gcgcaattat ctgccgtcgc acgagtgggt gctgaagggc ggctggaaca tcgccgattg
    11461 cgccgcgcgc agctacgacg tcgaggggat gcacgtcggc accgtcgcgg ccgggcggat
    11521 cggcagcgcg gtcctgcggc gcctgaagcc gttcgacatg cagctgcatt atttcgaccg
    11581 ccaccgcctg ccggcctcgg tcgagcagga gctcggagtc gcctggcacg acagcgtcga
    11641 ggacatgatc gccgtctgcg acgtggtcac catcaactgc ccgctccatc ccgaaaccga
    11701 gaatctgttc gacgcggcga tgatcgggcg gatgaagcgc ggcgcctatc tggtgaacac
    11761 tgcgcgcggc aagatcgtcg accgcgacgc ggtcgtcgcc gcgctcgcca gcggccagct
    11821 cgcggggtac gccggcgacg tctggttccc gcagccggcg ccgcgcgacc acccgtggcg
    11881 gacgatgccc aaccacggca tgaccccgca catctcgggc acgtcgctgt cggcgcaggc
    11941 gcgctacgcg gccggcacgc gcgagatcct cgaatgcttc ttcgagaacc gcccgatccg
    12001 cgacgaatat ctgatcgtcg agggcggccg cctcgccggc accggggcgc acagctacag
    12061 ccccggcgac gccaccggcg gctcggagga agcgcagaga ttcaaggccg agacgaccgc
    12121 ctgaacgctt atttcttgtc ctcgccgggg atcggggttt tcgtccccag cgcctcctgc
    12181 acgtagaggc gcttgacgag cacgtaggac accaccgtca gcggcgcggc gaggatgatg
    12241 ccgatgatcc cgaacagcat gccgaagccg agcagcgaga acaaaagcac caccggcgga
    12301 aggtcgaccg catattgctg caccaatggc tgcagcacat agccctcgaa ctgctggacg
    12361 ccgacgtaga gcgcgacggt ccaaagcgcc gtctcgggcc cggcgaccag cgccagcagg
    12421 acgccgggaa tcgccgcggc gatcggcccc gcgtagggga tgaactcgag cattccggcg
    12481 agcagcccga gcgccagcgc cgagggaacg ccgatcagcc acaggccgag gccggtcagg
    12541 gttccgacca ccaccatcgc gaacaattgc ccgcgcagcc acagccgaag cgcgcgctcg
    12601 ctgtcgtcca tcgcctcggc gaccaaggcg cgcttggact gcggcaccag cttgatcgct
    12661 ccgatccggt agaaattcgg ctgggcggcg aggaagatgc cgccgaagat caccaccaaa
    12721 gtgtcggcga tgccgttgcc gaccgacatc acgaccttgc caaggcccga tgcggcgcct
    12781 ccgccgccgg aacgcgcgct ctcgctcaga gcggcgagct gctcgccgaa gcccatcgcc
    12841 gcgatccgct cctccagcaa acgccaggcg gcaggcagcg cctcggtcag cacgtggacc
    12901 tgcgccgcga tctgcccgcc gaacacggtg accagcgccg cgatcgagcc gaggatcagc
    12961 ccgatcgaca gagcgaccgc gatcccgtcg ggcagcctcg cccggtcgcg gatgacgtcg
    13021 gccagcgccc ggaagatgct tgcgacgacg accgccccga acagcatcaa caccaggctg
    13081 cgcagctgcc acagcaggaa ggcgaccgcg gcgagcgcga tgacgatcag cacccggtgg
    13141 acgaaaatcc ggtcgccgtc cggatcgcgg cgcccggtca tggcgccggc cagccctcga
    13201 tgatctcgac gaagcgggtc agccaggcgt tggtctggtg gccgtcgacg acgcggtggt
    13261 cgatggtcag agtcacatag gccatcggca ggatgacgat cgcatcgtcg ccgtcgactt
    13321 cgcgcaccgc gactcgcttt tcgagcttgc cgatgccgag gatcgccgcc tggccctggt
    13381 ggaggatgat tggcgcggcg agcagcgacc ccgacacgcc gtggttggag atggtgaagc
    13441 tgccgccgga cacgtcgccg ccggtgagct tgccttcgcg cgctctccga gtcaggtcgt
    13501 cgagcgccag gccgatctgc tcgaggccga ggtttccggc atcgcgcacc accgggacca
    13561 ccagcccttt ctcgcccaat gcggtgccga cgccgatgtc gatggtcggc gacaccgcga
    13621 tccggtcctg ctcccaccgg ccgttgatcg ccggcgcgac cgccatcgcc tctgcggcgg
    13681 ccttgacgat gtaggcggtg tagctgagct tgacgccccg ccccgcttgt cgctccttgt
    13741 gtgccgcgac cgccgagaaa tcggcttcga acaccgcggt cacgtgcggc gcctgggcga
    13801 ccgcgcggac catgttctcg gcgatcttca gccgcatccg gtcgtgcggg atgtcggctc
    13861 ccgacggcgc cgcgccttcc aaggcgcgct cgacgtcctc gcgcgtaatg cgtccgcccc
    13921 gcccgcttcc cttgattcgc gacgggtcga tgccgtgctg ctgcagcgcc cgccggacgg
    13981 agggcgataa ccgtgtctcc ccctcctcat tcgtcccgag cgaagtcgaa gggccctgct
    14041 cggccgccgg tgcggaacgc ccttcgacgg gctcgggacg aacggactct ttcggatcga
    14101 tccgcccgag cagcgccccc ggcaccgcct cggcgtcggt gtcgagcacg atttccgcca
    14161 gcactcccgc ggcgggcgcc ggcacctcct gggtcacctt gtcggtttcg agctcgacca
    14221 aagggtcgtt ctcggcgacc gaatcgccga cctgcttgag ccacgcgcgg acgaccgcct
    14281 tggtgccctc ctgctcgtcg gggacgagga tgtcgatcac gtcagaaccc aaccagccgg
    14341 tcgatctcgg cgcggatccg tgtcaccgac ggcagcgcgg cttccatcag ggtcgggtga
    14401 tgcgggctcg ggatgtcggg catggtcacc cgcgcgaccg gcgcgtcgag gtcgaggaac
    14461 gcctcgtcgg cgaccaccgc ggcgatctcg gcgccgaagc cgccggtgcg cagatcctcg
    14521 tggacgacca ggcagcggcg ggtccgggcg acgctgtcga gcaccgtgtc gcggtcccac
    14581 ggcatcagcg tgcgcaagtc gatcacgtcc gccgacacgc cgtccgccgc cgcctcgcag
    14641 cgcggcacca tcgcgcccca ggtgacgatg gtgatctgct cgccctcgcg ggtcttcttg
    14701 gcgcgcccga acggcagcac ataatcgtcg cccggccacg gccgccgggc ccaggcgtcg
    14761 tcgagcatcg cgcggtgctc gaagaacagc accgggtcgt tgccgcgcag cgccccgcgc
    14821 agcagcccga ccgcgtcctc ggcgttggac ggcaccgcga ccagccagcc ggggttgtgg
    14881 acgaactgca cctcgttggt ctggctgtgc cacgggtcgc cgcacttgaa gaagccgccc
    14941 gggacgcgca gcaccagcgg ggcggcgaag cggttgtggg tgcgccagcg catcgtcccg
    15001 caatcgttga tctgctcggt cgccggctcg gcatatttgc ggaactggat ctcgggcacc
    15061 ggcatcagcc cggcgagcgc catcccgacc gcccggccga cgatgccctc ctcgttcagg
    15121 gacgtgtcga acacccgcgc gcgcccgaac ttttcctgca agcccagggt caccgcatgg
    15181 accccgccct tgggcccgat gtcctcgccg aacagcagca cccgcgggtt ggcctcgagc
    15241 tcctgctcga gcgtgcggcg gatcgcggtc accatgttga tccgctggcc gtcgggcctg
    15301 ggctcatcgc tcgacggcgg cggcgtaaag ccgtggttcc actgcccgcc gacctgctgc
    15361 agctcgcttt cgaaaaagac attgtcggtt acccgctcgg ggctcgacac gccgcgcgct
    15421 tccgcttcgg cgacggccgc cgcgacgttc gccgcggcgt ccgcttcgag cgccggccag
    15481 tcgagcttgg ccgcgaacgc cttcaatttg ggcagcgggt cgcgcgccca ctcggccttg
    15541 atctcggcct cgctcttgta gctttgggtg tcctggaagc tgtggccctc gagcctcggc
    15601 atggtcagcc gaagcagcgc cggagcgcgc tgggtgcgca cgtgccgcac cgcatcggcg
    15661 atcagccgcg ccgcctcggc cgggtcggtg ccgtcgccgt tgaatatggt gagccccgaa
    15721 aagctggcga ggttggcggc gatgtccttg cccggcgtct ggtactccga gggcaccgaa
    15781 atgccgtagc cattgtcctc gacgtacatc agcaggggca attgttgcgt tgttgcaatt
    15841 gttagcgccg accagaaccc gccggttgcg cagctcgcat cgccgccgag caccagagcg
    15901 atcgcgtcct ccggcccttg cccgagcact tcggccttgt aggcgatcgc ctgcgcccag
    15961 ccggccgcgg gcgtatattg cgcgccgact ccgccgctca tcggcagcgc gtgggcgccg
    16021 cccgggttgg ggtagttgaa cacgacgccg atgtcgcggc cgtccgaata gccaccggcc
    16081 agccccatgc ccgagcccag tgcgtcggcg agcgggactc ccagcgcgag cagcagcggc
    16141 cgcgagcgat agtagccgca ggccgcgtcg ccgtccctga gctgcagccc gagcagcacc
    16201 tgcgcgaggt cgtggccgcg ggccgagaat tggtagagga tcttcttgtt cgggacgagc
    16261 tcctgttcct cgacccggtc catctcgcgg ctggtgagga cgagccgggc gacctcgcgc
    16321 cagtcgacct cggcggcgac ccggcgggcg tcccgttcag ccatcgagcg cgtcgacgac
    16381 cgccgcgacg aaccggtcga tcgaatggtc ggccatcccg acgacgttga accggccgct
    16441 gtcggccatg tagatggcgt gccgctcgcg cagctcgagc acctgctcga ggctcagcgg
    16501 aagcatcgag aacatcccga actgggtgcc gatgaacgcc agccgcgggt cggcggcggc
    16561 gatccgccgg cgcactccgt tgatgcggcc gcgcatcgcg tcgagctcgc cgtgccattg
    16621 cgcgcgaagc tccggctcgt cgaggatgat ccgtaccgcc gcggcgccat ggtcgggcgg
    16681 catcgaccac atctcgcgcg cccgctggaa cacgtgcgcc atcgcccgag cggtcgcctc
    16741 ggccgagccg gtcttgacga acagcgaccc gacgcggtcg cggtagacgc tgaaattctt
    16801 gtcgcagctc tgcgcgacca ggacgtcgtc gcaggccgcc agaatgccgc gcagcccgcc
    16861 cgagtcctcg tcgagcccgc ggccgaagcc ctggtaggcg atgtcgatga acggcagcag
    16921 cccgcgctcg gcgatcagcc gggcgacctt ggcccactgc tcgtcattga ggtccgcccc
    16981 ggtcgggttg tggcagcagc cgtgcagcag cacgacgtcg ccttcgcgcg cggtaccgag
    17041 cgtttcgagc atgtcctcga accggaccgt gccgctggcg cggtcgtaat agggatatgc
    17101 gatcatctga aggcgggcgc cgcggatgat cggcggatgg ttgggccagg tcggctggct
    17161 gacgaacacc cgcgcatccg gattggcggt cgcgaccagt tcgaagccca ggcgaagtgc
    17221 gccgcagccg cccggggtct gcactccggc gatccgctcg tcgtcggcat ggctcccgag
    17281 caggatcggc cgcaacagct cggcgaaacg tgtgtcgccg gcgcttccga gataggcctt
    17341 ggtcggctgc gtctccagga ggattcgctc ggcctttttc atcaccttga ggatcggggt
    17401 ctcgcccgcc gcgtcgcgga acaccccgac cccgacgtcg atcttgtcgg gccgcgggtc
    17461 ggcgttggcc agagcgatca gtgcgagcag cgaatcgctg gtcaccggcg gcaggtcggc
    17521 cagcccccct ctcgcggaat tcggcgcctt ggcgtgggtt tcggtcagca tggcaaagcg
    17581 cttagcgaca aagtcgctgg cgcaaaagtc aggcgtcgta gtccacggcg accccctcgc
    17641 cgaccggcac cgactggcag gtcagcacat agcccgccgc gacctcctcg tcggtgaggc
    17701 cgtagcgcgc cgccatctcg acctttccgc tggtcacctt cgcccggcag gtcgcgcaca
    17761 cgcccgcctt gcacgcgaac ggcgccggca gcccggcggc gcgcgcgctg tcgaggatat
    17821 tgccttgcgt aaactcgacc ttgcgggtgc gcccgtcgag agtcaccgcg acgctcgccc
    17881 ccgccgccct ggtctgcagg tcggcgatct cgcgcgcgac cgcttcgggc gggcggtcgg
    17941 cggtgaagcg ctcgatatgg atccggtcct tcgcgacccc gcgctcgagc agcgccgctt
    18001 cggccgcgtc catcatcggc ccgggcccgc agatgaacca cgcgtcgacg ctttcaggct
    18061 cgccgaccag atgctcgacc gcatcgccgc acgtcgcctt gtcgagcatg ccgttgaaca
    18121 gctcgacctc gccttcctcc tcggcgagga aatggtagag ctcgaagcgg cccagatagc
    18181 ggtccttgag gtcggcgagc gcgtccagga agatgatcga ggcgctgtcg cggttgccat
    18241 agaacagggt gaagcggctt cccggttcct tcttcaatgc ggtgcggatc agcgaaatca
    18301 ccggagtgac ccccgaaccg ccggcgaagc cgacgtagcg gcgctgcttc gccgggtcga
    18361 actcgacggt gaagctgccg tgcggcggca tgacgtcgat cacgtcgccg gccttcagat
    18421 ggtcgccgac ccaattggag aagatgccgc cggcgatccg cttgacggtc accatccagt
    18481 cctgctcgtc gggcgcggtg cacagcgaat aattgcgccg cacctcctcg ccgtcgatct
    18541 ccgctctcag cgtcaaatgc tggccggctc ggaaggcgaa cttctcgcgc agctcgggcg
    18601 ggatttcgaa gcggatggaa ttggcttcgc tggtctcggg aaccacctcg gccacccgca
    18661 gcgcgtggaa atgttcggcc atcctaccag gtcgcgtcgg ggtaggttcg cggcaggaac
    18721 tgcatcaccg cgaggatatg gccgagatgc tcgctatggt ggccgcgccg cccgccgagg
    18781 atcggccgct gctcggccgg cgcctcgagt ttcgcatccg ccagcaccgc ggccagggcc
    18841 gagcgatact cgtcctcgaa agctcgcggg tcggccgcga ttccgcgctc gaccagcccg
    18901 ccgagcagct cgtccacctc gaacaattca gggatgaacc gccagcacca ctcaagcccc
    18961 tcgctcatcc gccgggcgct ttcctcggtg ccgtcgccga gccgcaccgt ccattcggac
    19021 accagctcgc ggtggtaggc gacctccttg accgccttgg ccgcgatgtc gcgcaggaat
    19081 gcgtcgctcg actccgccag ccgctggtac agcaggtgct ggtacgtcga gaacaataac
    19141 tgccgtgcga tcgtccggcc gaaatcgccg ttgggctgct cgaccagcag gcaattgcgg
    19201 aaatcgagca cgtcgcggtg gaacgcgagc cggtcggcat cgcccgccgc cccgagcaaa
    19261 tgcgtcgcct gcccgatcag gtcgagcgcg aggttggcga ggctcaggtc gacctcgagc
    19321 gccggcgcat ggccgcacca ttcgccgagg cgctggccga ggatcagcgc atcgtcgccg
    19381 agccgcagca gatagtcgaa ccgcgccggg tcgggctcgc ccgcggccgg agcgtcgaac
    19441 gcgcccgcgc cggtcgcgct cctgggcgta tcgatgggag gcagtgaggc caaaacaaac
    19501 tccgttcggg ctgagcctgt cgaagccctg tccttctcct tggcattaag aacaagtgca
    19561 gcccttcgac aagatcaggg cgaacggggg ctgggaagct agatgtgctt cacctcgtcc
    19621 ggaatgtcgt agaaggtcgg gtggcggtag atcttgtcgg cggccggctc gaacaacggc
    19681 ccggcctcat cgggatcgga ggcgacgatc tcggccgagg gcaccaccca caggctcacc
    19741 ccttcctgcc gccgcgtata ggtgtcgcgc gcgtggcgga gcgcgagctc cgcgtcagga
    19801 gcatgaacgc tgccaacgtg gcgatgcgaa aggccgccct tggagcggac aaagacctcc
    19861 cagagcggcc agtcactcac gcggcctttc gctcccgccg cttcgcttcg tgcgcagcgg
    19921 ccgcctcgcg gacccaggcg ccgtcgtccc acgccgcgcg gcgagcggcc atccgctcct
    19981 tggcgacggg gccttcgccg cgcacgaccg cgtagaactc gtcccagtcg acctcgccga
    20041 aatcgtagcc gccgcgcccg tcgttcttgc cctcgttcca cctgaggtcc gggtccggca
    20101 ccttcaaatc gaggaagtcc gcctgcggca ccgtgatgtc gacgaatttc tgccggagct
    20161 cgtcattggt ctcgcgcttg atccgccagc ggatcgactg cgcgctgttg ggtgaattgt
    20221 cgtccggcgg gccgaacatc atcaggctcg gccaccacca gcggttgagc gcgtcctgcg
    20281 ccatccgctt ctgctcggcg gtgccgttcg ccatggcgat catgatctcg tagccctggc
    20341 gctggtggaa gctttcctcc ttgcacaccc ggaccatcgc ccgggcgtag gggccgtagc
    20401 tggtgcgctg cagcggcacc tggttcatga tcgcggcgcc gtcgaccagc cagccgatcg
    20461 ctccgatgtc ggcccaggtc agcgtcgggt aattgaagat cgtcgaatat ttggctttgc
    20521 ccgagtgcag cgcctcgatc atctgctcgc ggctggtgcc cagcgtttcc gccgcgcagt
    20581 agaggtagag cccgtgcccg gcctcgtcct ggaccttggc gagcaggatc gccttgcgcc
    20641 gcaacgaggg cgcacgcgtg atccaactgc cctccggctg catcccgatg atttcggagt
    20701 gcgcatgctg ggcgatctga cggatcatcg tgcgccggta cgcgtcgggc atccagtcgc
    20761 gcggctcgat gcgcgaatcc tgtgcgatga gttcgtcgaa gcgggcctgt gcggcctcgt
    20821 cgcggtgctc atcgagactg gccacgct
//
LOCUS       DASTZO010000096         4354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_164308,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000096 DASTZO010000000
VERSION     DASTZO010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4354
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164308"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="VFU20_02670"
     CDS             <1..897
                     /locus_tag="VFU20_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HEU5285398.1"
                     /translation="EAVECAIKTARRYHHSKGSPHKHDLITFSNAFHGRTMATISATD
                     QSKLRDGFAPLLEGFRVVEFDDLDAALAAVDDHTAGFLVEPIQGEGGIRPASQEFMSG
                     LRAACDERDLMLVLDEVQCGVARTGTLYAYEQYGIEPDILATAKGIGGGFPLGACLAT
                     EKAAAGMVVGTHGSTFGGNPLAMAAGQAVLDVVANEEFLARVRQTGERLRSALEQMIP
                     NHDHLFAGVRGMGLMLGVKMKSDSRAFVNFLRTRGLLTVAAGDNVMRVLPPLIIEERH
                     IREFVDALSAAAADYEVPAAAA"
     gene            941..1843
                     /locus_tag="VFU20_02675"
     CDS             941..1843
                     /locus_tag="VFU20_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEU5285399.1"
                     /translation="MQTIELGTDVALGVTIPARNARGRLARIGPVLEQVLANHGYPPA
                     IERLLAEALVLTALLGSLLKDPTGQTTLQAQTENGIVDLLVCDYLGGELRGYVRHDPE
                     RLAEAGPQPSLFALFGKGYLAITFDQPATDERYQGIVPLEGTSLAEAAQSYFTQSEQI
                     PSIVRLAAEKRAAGWSAGGLLFQHLPEGEEGRDRLHTRLDHPDWPHVAILAGSAKPDE
                     LTDPGLALDDLAWRLFHEEDEVRTLEPLRLTRGCRCNHDYIRSVIARFPADERQAMVG
                     EDGLIRVDCAFCSLTFPIALDEVP"
     gene            1906..2307
                     /locus_tag="VFU20_02680"
     CDS             1906..2307
                     /locus_tag="VFU20_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEU5285400.1"
                     /translation="MRRMRTVAAVGAGGAALVLLVAATRPSALAKTSPGLWELSGLPN
                     AGTAFRQCVADTAALARVEHRGHSCTQVVIRDTPLSTEIHYTCTNGGFGRSKLTVLTP
                     RSLRIETQGISAQYPFNYTIQARRIGDCTAH"
     gene            2509..2859
                     /locus_tag="VFU20_02685"
     CDS             2509..2859
                     /locus_tag="VFU20_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HEU5285401.1"
                     /translation="MLYLLVKAAVSGVLIAVISEVARKNPGWGGLLASLPLVSLIAIL
                     WLWRDTHDPQRVAEQAISTFWFVLPSLPLFLILPLLIRAGWGFWPAILLSCLVTLGLY
                     AAMFAVSARLGVRL"
     gene            2856..3539
                     /gene="queC"
                     /locus_tag="VFU20_02690"
     CDS             2856..3539
                     /gene="queC"
                     /locus_tag="VFU20_02690"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HEU5285402.1"
                     /translation="MKRSAVVLLSGGLDSMVCAALAREAGFEVLALTIDYGQRHRVEL
                     AAAKAIAARLADRHIVLPLDLTTFGGSALTGGADVPKDSLGGGIPVTYVPARNTIFLS
                     LALAWAEATGARDLFIGVNALDYSGYPDCRPEFVAAFEALANRATKAGVEGTGFTVHA
                     PLLRMSKADIAREADRLGLDAALSHSCYDPAPDGSHCGHCDACLLRSKGFAEAGLPDP
                     TAYAQAAER"
     gene            3542..4174
                     /gene="queE"
                     /locus_tag="VFU20_02695"
     CDS             3542..4174
                     /gene="queE"
                     /locus_tag="VFU20_02695"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEU5285403.1"
                     /translation="MAYAVKEAFLTLQGEGVQSGRRAVFVRFAGCNLWSGREADRAAA
                     QCTFCDTDFVGTDGPGGGKFASAGELAAHVAGLWGESEEGRLAVITGGEPMLQLDPAL
                     VDALHALGFRVAVESNGTLAAVPGLDWLCVSPKAGTEVVQRSGDELKLVWPQPGIDPA
                     ELERWDFRHFLVQPMDCDQRDAAVAAAIELAMERPRWRLSLQAHKVVGLP"
BASE COUNT          611 a         1551 c         1502 g          690 t
ORIGIN      
        1 gaggcggtcg agtgcgcgat caagaccgcg cgccgctacc accattccaa gggcagcccg
       61 cacaagcacg acctgatcac cttctccaac gccttccacg gccggacgat ggcgaccatc
      121 agcgccaccg accagtcgaa gctgcgcgac ggcttcgcgc cgctgctcga gggcttcagg
      181 gtggtcgagt tcgacgacct cgacgcggcc ctggcggcgg tcgacgacca caccgccggc
      241 ttcctcgtcg agcccatcca gggcgagggc ggcatccgcc cggcgagcca ggagttcatg
      301 agcggccttc gcgccgcctg cgacgagcgc gacctgatgc tggtgctgga cgaggtccag
      361 tgcggggtcg cgcggaccgg cacgctctac gcctatgagc aatacggaat cgagcccgac
      421 atcttggcga ccgccaaggg catcggcggc ggcttcccgc tgggcgcctg cctggcgacc
      481 gagaaagcgg cggccgggat ggtcgtcggc acccacggct cgaccttcgg cggcaacccg
      541 ctggcgatgg cggcggggca ggcggtgctc gacgtcgtcg ccaacgagga gttcctcgcc
      601 cgcgtccggc agaccggcga gcggctgcgc tcggcgctcg agcagatgat ccccaaccac
      661 gaccatctgt tcgcgggcgt gcgcggcatg ggcctgatgc tcggggtcaa gatgaagagc
      721 gacagccggg cgttcgtcaa ttttctgcgc acccgcggcc tgctgaccgt cgcggcgggc
      781 gacaatgtga tgcgcgtgct gcccccgctg atcatcgagg aaagacatat ccgcgagttc
      841 gtcgacgccc tgtcggccgc cgccgccgat tacgaggtcc cggccgcggc tgcctgactc
      901 gagttgagcc tgcttggcgc cgcccctatc tagccgtcga atgcagacaa tcgaactcgg
      961 caccgacgtc gcgctcggag tcaccatccc ggcgcgcaac gcccgggggc gcctggcgcg
     1021 gatcgggccg gtgctcgagc aggtgctcgc caaccacggc tatccgccgg cgatcgagag
     1081 gctgctggcc gaggcgctgg tgctgaccgc gctgctcggc tccttgctca aggacccgac
     1141 cggccagacg acgctgcagg cgcagaccga gaacggcatc gtcgacctgc tggtgtgcga
     1201 ctatctcggc ggcgagctgc gcggctatgt ccgccacgac cccgagcgcc tcgccgaggc
     1261 ggggccgcag ccgtcgctgt tcgctctgtt cggcaagggc tatctggcga tcaccttcga
     1321 ccagccggcg accgacgagc gctaccaggg catcgtcccg ctcgagggca ccagcctcgc
     1381 cgaggcggcg cagagctatt tcacccagtc cgagcagatc ccgagcatcg tccggctcgc
     1441 cgccgagaag cgcgccgccg gctggagcgc cggcggcctg ttgttccagc atctgcccga
     1501 gggcgaggaa gggcgcgacc ggctgcacac tcggctcgac catcccgact ggccgcacgt
     1561 tgcgatcctc gccggctcgg ccaagcccga cgagctgacc gaccccgggc tggccctcga
     1621 cgacctcgcg tggcgcctgt tccacgagga ggacgaggtg cggactctgg agccgctgcg
     1681 gctgactcgc gggtgccgct gcaaccacga ttacatccgc tcggtgatcg cccggttccc
     1741 ggccgacgag cgccaggcga tggtcggcga ggacgggctg atccgggtcg attgcgcctt
     1801 ttgctcgctg accttcccga tcgccctcga cgaagtgccg taaggcggcg attcggagcc
     1861 gtccggactt ccggaaatta acgctcgcat ggtattgcgg tccgcatgag gcgaatgcgg
     1921 acggtcgcgg cagtgggcgc cggcggagcg gccttggtgc tgctcgtcgc cgcgacgcgc
     1981 ccctcggcgc tggccaagac cagccccggc ctgtgggaat tgagcggcct tcccaacgcc
     2041 ggcaccgcct ttcgccagtg cgtcgccgac accgcggcac tcgcccgggt cgagcatcgc
     2101 ggccacagct gcacgcaggt cgtgatccgc gacacgccgc tgtcgacgga aatccactac
     2161 acctgcacca acggcggctt cgggcgcagc aaactcaccg tcctgacccc gcggtcgctg
     2221 cgcatcgaga cgcagggaat ctccgcccaa tatccgttca attacaccat ccaggcgcgg
     2281 cggatcggcg attgcaccgc tcattaagcg ggcgtttaac cagcctgtcc taaaaggtcc
     2341 cggtgccgga ttggcacgct ttcctcccaa tcggacgagt aatgtccgat acctgggccg
     2401 ccacgtctcc ggacgcggcg gcccttcttt tctcgatcaa ggggcccccc acgtctccgg
     2461 acgcggcggc ccttcttttg gcggcggagg gggattagag ggcgctccat gctctatctg
     2521 ctcgtcaagg ctgccgtttc cggggtgctg atcgctgtga tttcggaagt ggcgcgcaag
     2581 aacccgggct ggggcggcct gctcgcctcg ctgccgctgg tttcgctgat cgcgattctc
     2641 tggctgtggc gcgacaccca tgacccgcag cgggtcgccg agcaggcgat cagcaccttc
     2701 tggttcgttc ttccgtcgct gcctctgttc ctgatcctgc cgctgctgat ccgcgccgga
     2761 tggggcttct ggccggcgat cctgctctcc tgcctggtga cgctcgggct ctacgcggcg
     2821 atgttcgcgg tgagcgcccg actgggggtc cggctgtgaa acggagcgcg gtcgtcctgc
     2881 tgtcgggcgg gctcgattcg atggtctgcg ccgcgctcgc ccgcgaagcg ggcttcgaag
     2941 tgctggcgct gaccatcgac tacgggcagc gccaccgggt cgagctggcc gcggccaagg
     3001 cgatcgccgc ccggctcgcc gaccgccaca tcgtccttcc gctcgacctc accacgttcg
     3061 gcggctcggc gctgaccggc ggcgcggacg tgcccaagga cagcttgggc ggggggatcc
     3121 cggtcaccta cgtgccggcc cgcaacacca tcttcctcag cctcgccctg gcctgggcgg
     3181 aggcgaccgg cgcccgcgac ctgttcatcg gcgtcaacgc gctcgattat tcgggctacc
     3241 ccgactgccg gcccgagttc gtcgcggcgt tcgaggccct ggccaaccgc gccaccaagg
     3301 ccggcgtcga ggggacgggg ttcaccgtcc acgcgccgct gctgcgcatg agcaaggccg
     3361 acatcgcccg cgaagccgac cgcctcgggc tcgacgcggc cttgagccac agctgctacg
     3421 acccggcgcc cgacggcagc cactgcggcc attgcgacgc ctgcctgctg cgctcgaaag
     3481 gcttcgccga agccgggctg cccgacccga cggcctacgc ccaggccgcg gagcgctgac
     3541 gatggcctat gccgtcaagg aagcgttcct gactctccag ggggagggcg tgcagtccgg
     3601 ccgccgggcg gtgttcgtgc gcttcgccgg ctgcaatttg tggtccgggc gcgaagcgga
     3661 ccgcgccgcc gcgcagtgca ctttctgcga caccgatttc gtcggcaccg acggccccgg
     3721 cggcggcaag ttcgcgagcg ccggggagct cgccgcccat gtcgccggac tgtggggcga
     3781 gagcgaggag ggccggctgg cggtgatcac cggcggcgaa ccgatgctcc agctcgatcc
     3841 ggcgctggtc gacgcgctcc acgcgctcgg cttccgggtc gcggtcgaaa gcaacggcac
     3901 cctggccgcg gtgcccgggc tcgactggct gtgcgtcagc cccaaggccg ggaccgaagt
     3961 cgtccagcgc agcggcgacg aactgaagct cgtctggccg cagccgggga tcgacccggc
     4021 cgagctcgag cgatgggatt tccggcattt cctggtccag ccgatggact gcgaccagcg
     4081 cgacgcggcg gtcgcggcgg cgatcgagct ggcgatggag cggccgcgct ggcggctgtc
     4141 gctgcaggcg cacaaggtcg tcggcctgcc ctgagggcag ccgatcagtc ggagttgccg
     4201 gcttccgttt cgtcgccgcg gttgtcgtcg gcgtcggctt caaccgccag attgtccgcc
     4261 tcgttcacgg tgaaattcac gtcggcggcc atgttggcgt cctcgccggt cgcggcgtcg
     4321 atcgacgtcg tgtcgttggc ggagatgttc cggg
//
LOCUS       DASTZO010000097         2279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_138263,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000097 DASTZO010000000
VERSION     DASTZO010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138263"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="VFU20_02700"
     CDS             complement(<1..227)
                     /locus_tag="VFU20_02700"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669381.1"
                     /note="catalyzes the formation of L-tryptophan from
                     L-serine and 1-(indol-3-yl)glycerol 3-phosphate; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU5285404.1"
                     /translation="MRLSGRFGRFGGTYVPEILMPALEQLEAAFLDAQDDPAFIAELN
                     HLLTHYAGRPTPLTRCRNLPDTIYLKREDLL"
     gene            complement(218..1600)
                     /gene="trpCF"
                     /locus_tag="VFU20_02705"
     CDS             complement(218..1600)
                     /gene="trpCF"
                     /locus_tag="VFU20_02705"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF006945.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HEU5285405.1"
                     /translation="MSEPSGLLGEIAAAKRAELAARFDGVSLDALRARAPPAERSLAA
                     AVGRPGARFILEIKKASPSAGPIRPGADPAEIALGYAGVADALSVLTDGTSFGGSIGD
                     LAAARSAFDGPILAKDFVLDPRQVAEARLAGADAVLVMLSLLDDRSARLMIAEAHRLG
                     MEALVEVHDEAEMGRALALDALLIGINNRDLRDLSVDLSTTERLAGLAPGRLVISESG
                     IACRGDVERLSPNADGFLVGSALMQAGEPAEAARALVFGRVKLCGVNRAEDLEAGRAA
                     THAGLVFVPESPRAVTLDQARSLAGGAAKAVGVFRDAPVRTVAEAAVELGLAAVQLHG
                     DEDSDTLRALRSVLPAGCEIWKAVTVDGTVASDCDAADRLLFDSGRGGTGRTFDWTLV
                     RGHPALGRSFVAGGIGPDNARAASRLGTYGLDVGSGIDALPGLKSADKIRALFEALRP
                     KGRQELRQCA"
     gene            complement(1593..>2279)
                     /gene="trpD"
                     /locus_tag="VFU20_02710"
     CDS             complement(1593..>2279)
                     /gene="trpD"
                     /locus_tag="VFU20_02710"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU5285406.1"
                     /translation="HGNRSFTSKCGSADVLEALGARLDASPQTSRRALDQAGFCFLLA
                     PQYHPGIAHAGPVRRALKVRTVMNLLGPCLNPARPPVQLLGVADPRLVEPVARTLLAL
                     SVERGLVVHGSGLDEVAPHGPTQAIRIADGALEPLTLTPDLAGVEAQPVQQIAGGAPA
                     ENAQRLVDLLEGRAGPADVAVVALNAGALLFTAGLADSFRLGADRAIDAIRSGLARQL
                     LARFVEASNE"
BASE COUNT          336 a          812 c          829 g          302 t
ORIGIN      
        1 tggagcaggt cctcgcgctt gaggtagatg gtgtccggaa ggttgcgaca gcgggtcagc
       61 ggcgtcggtc gcccggcata atgggtgagc agatggttga gctcggcgat gaacgccggg
      121 tcgtcctggg cgtcgaggaa cgcggcctcg agctgctcga gcgccggcat caggatttcg
      181 ggcacgtagg tcccgccgaa gcggccgaac cgcccgctca ggcgcattgc cgcaattcct
      241 ggcgcccctt cggccgcagc gcttcgaaca gcgcgcggat cttgtcggcg gacttcagcc
      301 cgggcagcgc atcgatcccc gatccgacat cgagcccata ggtgccgagc cgggacgccg
      361 cccgggcgtt gtccgggccg attcccccgg cgacgaacga tcggcccaag gccggatgac
      421 cgcggaccaa agtccagtcg aaggtccggc cggtgccgcc cctgccgctg tcgaacagca
      481 atcgatcggc ggcgtcgcag tcgctggcaa ccgtcccgtc gaccgtcacg gctttccaga
      541 tttcgcagcc ggccggcagg accgagcgaa gcgcgcgcaa ggtgtccgaa tcctcgtcgc
      601 cgtggagctg gaccgcggcg aggccgagct cgacggccgc ctcggcgacc gtccggaccg
      661 gcgcatcgcg aaacaccccg accgccttcg cggcgccccc cgccaggctt cgcgcctggt
      721 ccagagtgac ggcccgcggg ctttccggaa cgaagactaa gccggcatgc gtggcggcgc
      781 ggcccgcttc caggtcctcc gcccggttga ccccgcacag cttgacccgt ccgaacacca
      841 gcgcgcgggc cgcctcggcg ggctcgcccg cctgcatcaa cgcgctcccg accaggaagc
      901 cgtcggcgtt cggcgacaac cgctcgacgt cgccgcggca agcgatgccg gattcggaaa
      961 tcaccaggcg gccgggcgcg aggcccgcca gccgctcggt ggtcgacagg tcgacggaca
     1021 agtcgcgcag gtcgcggttg ttgattccga tcagcagtgc gtcgagggcc agcgcgcggc
     1081 ccatctcggc ctcgtcgtgg acctcgacca gggcctccat gccgagcctg tgcgcttcgg
     1141 cgatcatgag tcgcgcggaa cggtcgtcga gcagcgacag catcaccagc acggcgtcgg
     1201 ccccggcaag gcgcgcttcg gcgacctggc gcggatcgag gacaaagtcc ttcgccagga
     1261 tcggaccgtc gaatgcggat cgcgcggccg ccaggtcgcc gatcgatccg ccgaaggacg
     1321 tcccgtcggt cagcacgctc aaggcgtcgg cgacgccggc atagccgagc gcgatctcgg
     1381 cggggtcggc gcccggacga atcgggccgg ccgacggcga cgccttcttg atttcgagga
     1441 tgaagcgcgc gccgggccgg ccgacggcgg cggccaggct ccgttccgcc ggcggcgcgc
     1501 gcgcccgcag cgcgtcgagc gacacgccgt cgaaccgcgc cgccagttcg gcgcgcttgg
     1561 cggcggcgat ctcgccgagg agcccgctcg gttcactcat tgctggcctc gacaaagcgg
     1621 gccagcagct gccgcgcgag tccggagcgg atggcgtcga tcgcccggtc cgcgccaagg
     1681 cggaagctgt ccgccagccc ggcggtgaac agcaacgccc cggcattcag agcgaccacg
     1741 gcgacatcgg ccggccccgc gcggccttcg agcaggtcga cgagccgctg ggcattttcg
     1801 gccggtgcgc cgcccgcgat ctgctggacc ggctgcgcct ccaccccggc caggtcgggc
     1861 gtcagggtca gcggttccag cgctccgtcg gcgattcgaa tggcctgggt cgggccatgg
     1921 ggagctactt cgtcgaggcc gctgccgtgc acgaccagcc cgcgttcgac gctcagcgcg
     1981 agcaaggtgc gggcgaccgg ctcgaccagc cgcgggtcgg cgacgccgag caattggacg
     2041 ggcggccgcg cggggttcag gcaggggccg agcaggttca tcaccgtgcg caccttgagc
     2101 gcccggcgca cgggtccggc atgggcgatg ccgggatggt attgaggcgc gagcaggaaa
     2161 cagaagccgg cctggtcgag ggcgcgccgc gatgtttggg gcgaggcgtc gagccgcgct
     2221 ccgagcgcct cgagcacgtc ggccgagccg catttcgacg tgaacgagcg attgccatg
//
LOCUS       DASTZO010000098         3672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1130471,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000098 DASTZO010000000
VERSION     DASTZO010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3672
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1130471"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1014
                     /locus_tag="VFU20_02715"
     CDS             <1..1014
                     /locus_tag="VFU20_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5285407.1"
                     /translation="ELLRRADFISLHTPLTDQTRGILSREALAKTKPGVRIVNCARGG
                     LIDEAALKEGLESGHIAGAALDVFAVEPPPADHPLFGTRNFICTPHLGASTNEAQVNV
                     AIQVAEQMVDFLLLGGITNAVNMPSLSAEEAPRLKPYMALAEKLGRLVGQVVGTQVRS
                     VDVEVEGAAAELNIKPITGAVLAGLMGTYSDTVNMVNAPFLAKERGLDVREIRHDREG
                     DYHTLLAVTVGTGDGKRRVAGTLFGNRAPRLVDIFGIQVEAELGGQMIYIVNTDAPGF
                     IGALGTALGQHGVNIATFNLGRRAAGGEAVALVAVDDPIGPELAEELARLPGVLQVVP
                     LSF"
     gene            1067..2356
                     /locus_tag="VFU20_02720"
     CDS             1067..2356
                     /locus_tag="VFU20_02720"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU5285408.1"
                     /translation="MGNVTVIGAQWGDEGKGKIVDWLASRADMVVRFQGGHNAGHTLV
                     VGDQTYKLSLLPSGIVRGTPSVIGNGVVLDPWALKAEVERVAAQGVAVTPDVLMIAET
                     CPLILPIHRDLDALREDASGAGKIGTTRRGIGPAYEDKVGRRAIRVCDLAHLAELDPL
                     LDRLCAHHDALLAGFGKPPVDRERLRSDLAGIADFVLPFAKPVWRDLDEALRRGRRIL
                     FEGAQGVLLDVDHGTYPFVTSSNTVAGTTGAGSGIGPSAAGFVLGIVKAYTTRVGSGP
                     FPTELDDETGQRLGERGHEFGTVTGRRRRCGWFDAVLVRQAVTVSGVTGVALTKLDVL
                     DGFDTVRICTGYRIGGEAFEYLPPHAFDQERVEPIYEEIEGWPGSTRGARSWADLPAR
                     AIKYVRRIEELIRCPVALVSTSPERDDTILVRDPFAG"
     gene            complement(2353..2631)
                     /locus_tag="VFU20_02725"
     CDS             complement(2353..2631)
                     /locus_tag="VFU20_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEU5285409.1"
                     /translation="MAEAVGRKVRIYGRVQGVFFRQWTVDRARSLGVAGWVRNASDGS
                     VEAHLAGDQSAVAQLIEHMRRGPPQARVEDLTVETVEPEPVEGFSVKL"
     gene            complement(2638..3429)
                     /gene="fabI"
                     /locus_tag="VFU20_02730"
     CDS             complement(2638..3429)
                     /gene="fabI"
                     /locus_tag="VFU20_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008829650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEU5285410.1"
                     /translation="MAGKRGLIMGLANDRSLAWGIAKALGEQGAELAFSYQGETLEKR
                     VRPLAEQLGSDLLIDCDVSSNEALDTAFAALAERWGKLDFLVHAIGYSDKNELRGKFV
                     DTSLDNFLMTMNISVYSFVAVVRRARAMMDSGGSLLTLTYYGAEKVIPHYNVMGVAKA
                     ALETSVKYLAMDLGPEGIRVNAISAGPIKTLAASGIGDFRYIMKWNEYNAPLRRNVTI
                     EDVGGAALYLLSDLASGVTGEVHHVDAGYNVVGMKAEDAPDIATV"
     gene            complement(3462..>3672)
                     /locus_tag="VFU20_02735"
     CDS             complement(3462..>3672)
                     /locus_tag="VFU20_02735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285411.1"
                     /translation="GVMIALIFFFVIGLGVVMGAELNAALAEGGATALKGEVYSGPFK
                     DELEVEEPQPGEDVPAELETGGPQL"
BASE COUNT          551 a         1279 c         1263 g          579 t
ORIGIN      
        1 gagcttctgc gtcgcgccga cttcatctcg ctgcacaccc cgctgaccga tcaaacgcgc
       61 ggcatcctgt cgcgagaggc gctggcgaag accaagccgg gcgtacgcat cgtcaattgc
      121 gcgcgcggcg gcctcatcga cgaagcggcg ctcaaggaag gtctcgagag cgggcatatc
      181 gccggagccg cgctcgacgt tttcgcggtg gagccgccgc cggccgatca cccgctgttc
      241 ggcacccgca acttcatctg cacgccgcac ctcggtgcct cgacaaacga ggcgcaggtg
      301 aacgtcgcga ttcaggttgc cgagcagatg gtggacttcc tgcttctcgg cgggatcacc
      361 aatgcggtga acatgccctc gctgtcggcc gaggaagcgc cgcggctgaa gccctacatg
      421 gcgctcgcgg aaaagctcgg gcggctggtc ggccaggtgg tcgggacgca ggtgcgctcg
      481 gtcgacgtcg aggtcgaggg cgcggccgcc gagctcaaca tcaagccgat caccggagcg
      541 gtgctggccg ggctgatggg cacctattcg gacacggtga acatggtcaa cgcgccgttc
      601 ctggccaagg agcgcgggct cgacgtgcgc gagatccgcc acgaccgcga gggcgactat
      661 cacacgctgc tcgcggtcac ggtgggaacc ggggacggca agcggcgggt cgccgggacc
      721 ttgttcggca accgggcgcc gcggctggtc gacatcttcg gaatccaggt cgaggccgag
      781 ctcggcggcc agatgatcta catcgtcaac accgacgcgc ccggcttcat cggcgcgctc
      841 ggcaccgcgc tcggccagca cggagtcaat atcgccacct tcaacctcgg ccgacgcgcg
      901 gcgggcgggg aagcggtcgc gctggtcgcg gtcgacgacc cgatcggccc ggagctcgcc
      961 gaggagctgg ctcggctccc aggcgtgctg caggtcgtgc cgctgagctt ttgaccgcgt
     1021 cccccgcctc tcctagagcc gcaacccgtc agaaggggca ctgagcttgg gtaacgtcac
     1081 cgtcatcggc gcgcagtggg gcgacgaagg caagggcaag atcgtcgact ggctcgccag
     1141 ccgcgccgac atggtggtgc gcttccaggg cggccacaac gcgggccaca cgctcgtcgt
     1201 cggcgaccag acctacaaat tgtcgctgct cccctcgggg atcgtccgcg gcacgccctc
     1261 ggtgatcggc aacggagtgg tgctcgaccc gtgggcgctg aaggccgagg tcgagcgggt
     1321 cgcggcgcag ggcgtcgccg tcacccccga cgtgctgatg atcgccgaga cctgcccctt
     1381 gatcctgccg atccaccgcg acctcgacgc gctccgcgag gatgcgtccg gggccggcaa
     1441 gatcggcacc acccggcgcg gcatcggccc ggcctatgag gacaaggtcg ggcgccgggc
     1501 gatccgggtc tgcgacctcg cccatctggc cgagctcgac ccgctgctcg accggctgtg
     1561 cgcccaccac gacgcgctgc tcgccggctt cggcaagccg ccggtcgacc gcgagcggct
     1621 gcgttccgac ctcgccggga tcgcggattt cgtgcttccg ttcgcaaagc cggtgtggcg
     1681 cgacctcgac gaggcgcttc ggcgcggccg ccggatcctg ttcgagggcg cgcaaggcgt
     1741 gctgctcgac gtcgatcatg gcacctaccc gttcgtcacc tcctccaaca ccgtcgccgg
     1801 gacgacgggc gcgggcagcg gcatcggccc gtcggcggcc ggcttcgtgc tcgggatcgt
     1861 caaggcctac acgacgcggg tcggctccgg gcccttcccg accgagctcg acgacgagac
     1921 cggccagcgg ctgggcgagc gcggccacga gttcggaacc gtcaccggcc ggcggcggcg
     1981 ctgcggctgg ttcgacgccg tgctggtccg ccaggcggtc acggtgtccg gcgtcaccgg
     2041 ggtcgctctg accaagctcg acgtgctcga cgggttcgac acggtgcgga tctgcaccgg
     2101 ctaccggatc ggcggcgagg cgttcgaata cctgccgccg cacgccttcg accaggagcg
     2161 cgtcgagccc atttacgagg agatcgaagg ctggccgggc tcgacccgcg gggcgcgcag
     2221 ctgggccgac ctgccggccc gagcgatcaa atatgtccgc cgaatcgagg aattgatccg
     2281 ctgcccggtg gcgctggtga gcacgtcccc cgagcgcgac gacacgatcc tcgtgcgcga
     2341 cccgttcgcg ggttagagct tcaccgaaaa gccttcgacg ggctcgggct cgaccgtctc
     2401 cacggtgagg tcctcgacgc gggcctgggg cgggcctcgg cgcatgtgct cgatcagttg
     2461 cgcgaccgcg ctctggtcgc cggcgagatg cgcctcgacc gagccgtcgc tggcgttgcg
     2521 cacccagccg gcgactccca gcgagcgcgc gcggtcgacc gtccactgcc tgaagaagac
     2581 gccctggacg cggccgtaga tgcgcacctt gcggccgacg gcttcagcca cggcggctca
     2641 caccgtggcg atgtcgggcg cgtcctcggc cttcatcccg acgacattgt agccggcatc
     2701 gacgtggtgg acctcgccgg tgaccccgct ggcgaggtcg gacagcaggt agagcgcggc
     2761 tccgccgaca tcctcgattg tgacgttgcg gcgcagcggg gcgttatatt cgttccactt
     2821 catgatgtag cggaagtcgc cgatgccgct tgcggccagc gtcttgatcg gcccggccga
     2881 gatcgcgttg acccggatgc cctcggggcc gaggtccatc gccagatatt tgacgctggt
     2941 ctcgagcgcc gccttggcga cccccatcac attgtagtgt gggatcacct tctcggcgcc
     3001 gtaataggtc agggtcagca gcgagccgcc gctgtccatc atcgcccgcg cgcgccggac
     3061 gacggcgacg aagctgtaga ccgagatgtt catggtcatc aggaaattgt cgaggctggt
     3121 gtcgacgaac ttgccgcgca gctcgttctt gtcggaatag ccgatcgcgt ggaccaggaa
     3181 gtcgagcttg ccccagcgct cggcgagcgc cgcgaaggcg gtgtcgagcg cctcgttgct
     3241 gctgacgtcg cagtcgatca ggaggtcgga gcccaattgc tcggcgagcg gccggacgcg
     3301 cttctcgagc gtctcgccct ggtaggagaa ggccagctcg gcgccctgct cgcccagtgc
     3361 cttggcgatt ccccaggcga gcgagcggtc gttggcgagg cccatgatca gcccgcgctt
     3421 gccagccatg attccactgg atcccttggc cgaatctttg ctcaaagctg tggcccccct
     3481 gtctcgagct cggcaggcac gtcctcgccc ggctgcggct cctccacctc gagctcgtcc
     3541 ttgaacggcc cgctgtagac ctcgcccttt agcgcggtgg cgccgccttc cgccagcgcc
     3601 gcgttgagct cggcacccat tacaacgccg agtccgatca cgaagaagaa gatgagcgcg
     3661 atcatcactc cg
//
LOCUS       DASTZO010000099         2695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_582601,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000099 DASTZO010000000
VERSION     DASTZO010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_582601"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VFU20_02740"
     CDS             <1..529
                     /locus_tag="VFU20_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HEU5285412.1"
                     /translation="IFAPGVTIIDDPLRMRGLRSRPFDGEGVRVARMELVSDGVLTSW
                     IAESASARQLGIRPTGHAARGAGGAPGASPSNLYLAAGPRSRDELLAAFPEAILVTEL
                     IGQGANGVTGDYSRGAAGFMVRGGVIAEPVAEITIASNLTDMFATLEPASDLEFRRGV
                     DTPTVLVPEMTVGAA"
     gene            complement(570..1421)
                     /locus_tag="VFU20_02745"
     CDS             complement(570..1421)
                     /locus_tag="VFU20_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285413.1"
                     /translation="MNTRLKWVIAAAATTILAGPALANNQWGNYHWPRSSNPVQLDIL
                     RQIGSQWDSAYVTAVADWEVSTVLSLNPASTNAGVSPKRCNPISGKILVCADRYGFRG
                     WLGLATIWIDGDHIVQGTTKLNDSYHNSPPYNSPAWRSLVMCQEIGHDFGLDHQDETF
                     DNANLGTCQDYTNNPSGPPSNEHPNSHDYDTLEAMYSHSDSAAIAGGGRPGNGSGAFA
                     FREVGNGPASATASGSAGDSPAEWGRAIRFDAHGRPNVYRMDLDGNRAKITHVLWVPG
                     FRPEGSR"
     gene            complement(1564..2007)
                     /locus_tag="VFU20_02750"
     CDS             complement(1564..2007)
                     /locus_tag="VFU20_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5285414.1"
                     /translation="MPLTRDKDIAELLRNARTIAMVGASDRPDRPSYGVMKFLQSHGY
                     RVIPVNPQITGEHVHGEYVWRELAQIGEPIDIVDIFRRPQAAGEAVDQAIFAGAKAVW
                     LQLGIVNEEAGQRAEQAGLKLVMDRCIKIEIMRLGVPAVGQSAQA"
     gene            complement(2035..>2695)
                     /locus_tag="VFU20_02755"
     CDS             complement(2035..>2695)
                     /locus_tag="VFU20_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEU5285415.1"
                     /translation="SGKKVGLIDADIYGPSQPRLLGSDAKPTAEGDRLIPVAAHGLHF
                     LSLGQLVSAGHALAWRGPMATGALARLVEADWGDAEYLIVDLPPGTGDVQLSLIQRSR
                     PDGAVIVSTPQDLSLIDATRAIDLFRKTKVPVLGIIENMAGYACPHCGETSDPFGSGG
                     AEAAASELGVPFLGRLPLTAALRSASDAGTPPAAGDGPEAEEFARLADKLLAALDTAA
                     E"
BASE COUNT          408 a          899 c          904 g          484 t
ORIGIN      
        1 tatattcgct ccaggagtga cgattatcga cgacccgctg cgcatgcgcg ggttgcgatc
       61 gcggccgttc gacggcgaag gcgttcgagt ggcgcgcatg gagctggtgt cggacggcgt
      121 cctgaccagc tggatcgccg aaagcgcatc ggcccgccag ctcggaatcc ggccgaccgg
      181 ccatgcggcc cgcggcgccg gcggggcgcc cggagccagc ccgagcaacc tctatttggc
      241 cgccgggccg cgctcgcgcg acgagctgct ggccgccttt cccgaggcca tcctggtcac
      301 cgagctgatc gggcagggcg ccaatggagt caccggcgac tacagccgcg gcgccgccgg
      361 cttcatggtc cgcggcggag tgatcgccga gccggtcgcc gagatcacca tcgcctcgaa
      421 cctcacagac atgttcgcga ccctggagcc cgcatcggac ctcgagtttc ggcgcggggt
      481 cgacaccccg accgtgctgg tgcccgaaat gacggtcggc gcggcctgac cgccgagcaa
      541 tttttcaggg tgcggcgagg acttcgagct tagcggctgc cctctggccg gaaccccggt
      601 acccagagga cgtgggtgat ctttgcccga ttgccgtcga ggtccatgcg gtagacgttc
      661 ggacggccat gtgcgtcgaa gcgtattgcc cggccccact ctgcggggga atcgccggcg
      721 ctgccgctgg ctgtcgcgct tgcaggtccg ttgccgactt cacgaaaggc gaaggctccc
      781 gaaccattcc ccggacgacc gccgccggcg atggccgcac tgtccgagtg cgaatacatg
      841 gcctcgaggg tgtcataatc atggctgttg gggtgttcgt tgctcggcgg gcccgaggga
      901 ttattggtat agtcctggca ggttcccagg ttcgcattgt cgaacgtctc atcctggtgg
      961 tcgaggccga agtcatggcc gatctcctgg cacatcacca atgagcgcca ggccggcgag
     1021 ttatacggcg gcgagttgtg atagctgtcg ttgagcttgg tcgtcccctg gactatgtga
     1081 tcgccatcga tccagatcgt ggcgagcccg agccagccgc ggaatccgta gcggtcggcg
     1141 cagaccaaaa tcttgccgct gatcgggttg catcgttttg ggctcactcc cgcgttcgtt
     1201 gacgcgggat tcaggctgag caccgtggag acttcccagt cggctaccgc tgtaacatag
     1261 gccgagtccc actgactgcc gatttgcctc agtatgtcga gttggaccgg attgctgctc
     1321 ctcggccaat gatagttccc ccactggttg ttggccaacg cgggtcccgc gaggatcgtc
     1381 gtcgctgctg cggcgatcac ccatttcagg cgcgtgttca tgactttccc ctggttgccc
     1441 cgcatcaaca cgcgcccgga cgactccggg ctcggccgca gagttcagac cgcaagttcc
     1501 tgccaaacga gtttcgagtc aatgcttcga gtggccactc tgttggagct gcggaggtcg
     1561 gaactatgcc tgcgccgatt ggccgacggc tggaactccg agccgcatga tctcgatctt
     1621 gatgcagcgg tccatgacca actttagccc ggcctgctcg gccctttgcc ccgcttcctc
     1681 gttgacgatg ccgagctgaa gccagaccgc cttggcgccg gcgaagatcg cctggtcgac
     1741 cgcttcgccg gcggcctgcg ggcggcggaa gatgtcgacg atgtcgatcg gctcgccgat
     1801 ctgcgccagc tcgcgccaca catattcgcc gtggacatgc tcgccggtga tctgcgggtt
     1861 gaccgggatc acccgatagc cgtgcgactg gaggaacttc atcactccgt aggacgggcg
     1921 gtccggccgg tcggatgctc cgaccatggc gatggtccgg gcattcctca gcagctcggc
     1981 aatgtccttg tcgcgggtca gcggcatggc ggctctcctt gccgcatcaa tgcgtcactc
     2041 ggcggcggta tccagtgccg cgagcaattt gtcggccagc cgagcgaact cctcggcctc
     2101 cggtccatcg ccggcggcgg gcggagtccc ggcgtccgac gccgagcgaa gcgcggccgt
     2161 cagcggcagg cggccgagga acggcactcc gagctcgctc gccgcggctt ccgcgccgcc
     2221 gcttccgaac gggtcggaag tctcgccgca gtgcgggcag gcatagccgg ccatgttctc
     2281 gatgatcccc agcaccggca ccttggtctt ccggaacagg tcgatcgccc gggtcgcgtc
     2341 gatcagcgac aggtcctgcg gggtcgagac gatcaccgcc ccgtcgggcc gcgaccgctg
     2401 gatcagcgac agctggacgt cgccggtgcc cggcggcagg tcgacgatca gatattcggc
     2461 gtcgccccag tcggcctcga ccagccgggc gagcgctccg gtcgccatcg gcccgcgcca
     2521 ggcgagcgca tggccggccg agaccaattg gccaagcgac aggaaatgca gcccgtgagc
     2581 ggcgaccggg atcagccggt cgccctcggc ggtcggtttg gcgtcgctgc cgagcagccg
     2641 gggctgcgac ggcccgtaga tgtcggcgtc gatcagtccg accttcttgc cggag
//
LOCUS       DASTZO010000100         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_371591,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000100 DASTZO010000000
VERSION     DASTZO010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_371591"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..651
                     /locus_tag="VFU20_02760"
     CDS             <1..651
                     /locus_tag="VFU20_02760"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU5285416.1"
                     /translation="SGKGVLNNRISEHIFTSLATIGVPTHFIRRLNMREQLIRQVEIV
                     PIEVVVRNVAAGSLSKRLGIEEGTQLPRTIIEYYYKDDALGDPLIADEHIACFGWASQ
                     DEMNDIADMAIRVNDFMCGMFAAIGIRLIDFKLEFGRVWENDYPRIILADEISPDGCR
                     LWDMKSNEKLDKDRFRLGLGGEAEAYQEIARRLGLLEDEEANSVLDFDTHRKKRGR"
     gene            768..>2870
                     /locus_tag="VFU20_02765"
     CDS             768..>2870
                     /locus_tag="VFU20_02765"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU5285417.1"
                     /translation="MTVPRARVVTLNAALGPLDYRIPDGMAPNGVLPPGSVVVAPLGP
                     RQLVGVAWEPERLPSEEVGDNRLRPLAALLDVPPIAAPLRRLAEWTADYYLSPLASVL
                     RMILPSSSALEGSRQLTEYRPTGLVPERMTPQRLKALAALEGRQGTIRELARHSEVSE
                     AVLRGLVHAGALEALTVEADRPLETPDPDFAPPDLNAEQRSAAASLSAAIGKGFDPVL
                     LDGVTGSGKTEVYFEAVAEALRQDKQALVLLPEIALTEPFLKRFQARFGCAPVAWHSD
                     LRSSQRRRAWRAIASGEARVTVGARSALFLPYRDLGLIVVDEAHEPSFKQEEGVQYHA
                     RDVAVMRGHFEHIPVILSSATPAIESRHMVEIGRYREVTLTRRYAGATLPEIRAVDMT
                     QDPPPRGRWLAPSLVEELRSNLVAGEQSLLFLNRRGFAPLTLCRHCGHRFQCPNCTAW
                     MVEHRLMHRLACHHCGHVMPPPRACPECGEEDSLVPVGPGVERIGDEVADLFPDARTA
                     IVTSDTIWSPARAAEFVAAMEGQAIDIVIGTQLVTKGYHFPNLTLVGVVDADLGLSGG
                     DLRAAERSFQQIQQVAGRAGRGDKPGRVLVQTHDPGAPVIAALVSGDGPGFYAAETEA
                     RREAAMPPFGRLAAIVVSAEDAEEAQAVARRIGQAAPRVEGMAVFGPAPAPLAMLRGR
                     HRQRILVHAARSLDVQDVI"
BASE COUNT          417 a         1046 c          981 g          426 t
ORIGIN      
        1 agcggcaagg gcgtgctcaa caaccgtatc tccgagcaca tcttcacgtc gctggcgacg
       61 atcggcgtgc cgacccactt catccgccgg ctgaacatgc gcgagcaact catccgccag
      121 gtcgagatcg tgccgatcga ggtggtggtg cgcaacgtcg ccgccggctc gctgtcgaag
      181 cgcctcggga tcgaggaagg cacccagctg ccgcgcacga tcatcgaata ttactacaag
      241 gacgacgcgc tcggcgaccc gctgatcgcc gacgagcata tcgcctgctt cggctgggcg
      301 agccaggacg agatgaacga catcgccgac atggcgatcc gggtgaacga cttcatgtgc
      361 ggcatgttcg ccgccatcgg catccgcctg atcgacttca agctcgagtt cggccgggtg
      421 tgggagaacg actatccgcg catcatcctc gccgacgaga tcagccccga cggctgccgg
      481 ctgtgggaca tgaaatcgaa cgagaagctc gacaaggacc gcttccgcct gggcctcggc
      541 ggcgaggccg aggcctatca ggagatcgcg cggcgcctcg gcctgctcga ggacgaggaa
      601 gccaacagcg tcctcgactt cgacacccac cgcaagaagc gcgggcggta aggttgcggg
      661 tcaccggcac ctccgttcgc cctgagcttg tcgaagggct gcccttcttc cttgcatagg
      721 aatagcaggg cttcgacagg ctcagcccga acggcttggt tgtgccggtg actgtccccc
      781 gcgcccgtgt cgtcactttg aacgccgccc tcgggccgct cgattatcgg atcccggacg
      841 gcatggcgcc aaatggcgtg ctgccgcccg gctcggtggt cgtcgctccg ctcggcccgc
      901 ggcagctggt cggcgtggcg tgggagcccg agcggctgcc gagcgaggag gtcggcgaca
      961 accgcctgcg cccgctcgcc gcgctgctcg acgtgccgcc gatcgccgcg ccgctgcgcc
     1021 gcctcgccga atggaccgcc gattattacc tttcgccgct ggcgtcggtg ctgcggatga
     1081 tcctgccgtc gtcgagcgcg ctcgagggct cgcgccagct aaccgagtac cgcccgaccg
     1141 ggctcgtgcc cgagcgcatg accccgcagc gcctgaaggc gcttgccgcg ctcgagggcc
     1201 gccaggggac gatccgcgag ctcgcccggc actccgaggt cagcgaggcg gtgttgcgcg
     1261 gcctggtcca cgcgggagcg ctcgaggccc tgaccgtcga ggccgaccgc ccgctcgaga
     1321 cccccgaccc cgatttcgcg ccccccgacc tcaacgctga gcagcgctcc gcagccgcca
     1381 gcctgtcggc cgcgatcggc aagggcttcg acccggtgct gctcgacgga gtcaccggct
     1441 cgggcaagac cgaggtctat ttcgaggccg tcgccgaggc tctccgccag gacaagcagg
     1501 cgctggtgct gctgcccgag atcgccctga ccgagccgtt cctcaagcgc ttccaggcgc
     1561 gcttcggctg cgctccggtc gcctggcact ccgacctgcg ctcgtcgcag cggcgccgcg
     1621 cgtggcgggc gatcgcaagc ggcgaggcca gggtcacggt cggcgcccgc tcggcgctgt
     1681 tcctgccgta ccgcgacctc ggcctgatcg tcgtcgacga agcccacgag ccgagcttca
     1741 aacaggaaga aggagtccag taccacgccc gcgacgtcgc ggtgatgcgc ggccatttcg
     1801 agcacattcc ggtgatcctg tcgtcggcca ccccggcgat cgaatcgcgc cacatggtgg
     1861 agatcggccg ctatcgcgaa gtgaccctga cccgccgcta tgcgggcgcc acgctgccgg
     1921 aaatccgcgc ggtcgacatg acccaggacc cgcccccgcg cggccgctgg ctggcgccga
     1981 gcctggtcga ggagctgcgc tccaacctcg ttgccggcga gcagtcgctg ctattcctca
     2041 accgccgcgg tttcgcgccg ctgaccctgt gccgccactg cggccaccgc ttccagtgcc
     2101 ccaattgcac cgcctggatg gtcgagcacc ggctgatgca ccgcctggcc tgccaccatt
     2161 gcggccacgt gatgccgccg ccgcgcgcct gccccgagtg cggtgaggag gacagcctgg
     2221 tgccggtcgg ccccggagtc gagcggatcg gcgatgaagt tgccgacctg tttccagacg
     2281 ctcggaccgc gatcgtcacc tcggacacta tctggtcgcc ggcccgcgcg gccgagttcg
     2341 tcgcggcgat ggaagggcaa gcgatcgaca tcgtcatcgg cacacagctg gtcaccaagg
     2401 gctatcactt tcccaacctc accctggtcg gggtggtcga cgccgacctc ggcctgtcgg
     2461 gcggcgattt gcgcgccgcc gagcgcagct tccagcagat ccagcaggtc gccggccgcg
     2521 ccgggcgcgg cgacaagccc gggcgggtgc tggtccagac ccacgacccc ggcgcgccgg
     2581 tgatcgcggc cctggtgtcg ggcgacggcc cgggcttcta cgccgccgag accgaggcgc
     2641 ggcgcgaggc ggcgatgccg ccgttcgggc gcctcgccgc gatcgtcgtc tcggcggagg
     2701 atgcggagga ggcgcaggcc gtcgcccggc gcatcggcca ggccgcgccc agggtcgagg
     2761 ggatggcggt gttcgggccc gccccggcgc cgctggcgat gctgcgcggc cgccaccgcc
     2821 agcggatcct ggtccacgcg gcgcgcagtc tcgacgtgca ggacgtgatc
//
LOCUS       DASTZO010000101         6136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1207494,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000101 DASTZO010000000
VERSION     DASTZO010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6136
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1207494"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VFU20_02770"
     CDS             <1..520
                     /locus_tag="VFU20_02770"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5285418.1"
                     /translation="AVGQLGDQPELRAISAAVVAAGLLAANRRLVRTGVRMLLAHEIA
                     TLAKDVIKRRIDRTRPHSARTEAERTAKPGRHTAKTKTSFPSGHSAGAIAVARALARE
                     YPGLQAPALAAAGIVAAAQVPRLNHYPSDVVAGLALGAASEAAADLVMRLGERLEDAA
                     TPEAPPQPRPRA"
     gene            complement(480..2777)
                     /locus_tag="VFU20_02775"
     CDS             complement(480..2777)
                     /locus_tag="VFU20_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEU5285419.1"
                     /translation="MKIACIGGGPAGLYFAISMKLRDPGHEVHVFERNPRGVTFGWGV
                     VFSDQTVENLTANDPQSATAIADEFAHWDDIDVHYRGQCIRSSGHGFIGIGRMRLLEI
                     LQQRAAELGVGLSFDHECDPDDPKWAEYDLVIASDGINSRVRDRFADAFEVDVDVRAN
                     KFIWLGTSKAFDAFTFAFEETDAGWIWAHAYKFAPDCSTFIVECSEAVWRGLGFDRID
                     QAEAIARCETIFAEYLDGHPLVSNAAHLPGPAAWLNFRRIKCERWSHGRFILLGDAAH
                     SAHFSIGSGTKLALEDAIKLADVLNRPGLELDAALFEYHAERSLEVLKLQNSARNSTE
                     WFETLERYLHFEPLQFAYSLLTRSQRISHENLRLRDKDWLESVERWFWERAGVPGKTA
                     APMFAPFRLRDMSVENRITVSPMAMYSAVDGTPGDFHFVHLAERALGGAGLVFTEMTC
                     VSPEGRISPGCTGMWNGEHLAAWRRIVDFVHSNSKARICLQLGHSGGKGSTRLGWEGN
                     DAPLDEGNWPVMAASDVPWSEANQRPRAMTRADMDMVREQFVAAVQMGLEAGFDMVEL
                     HAAHGYLLSSFITPLQNRRTDDYGGSLDNRLRYPLEVFAAMRAAWPPHKPMSVRISAS
                     DWAGDQGITPDDAVAIAEAFAQAGADLIDVSAGQTWTGAQPVYGRMFQTPFSDKLRNE
                     ARVATMAVGNIYEPDHANSILAAGRADLVALGRPHLIDPMWTLRAAAALDYRDIHVPP
                     QYLNGQAQLARNLKREAEAAAALRV"
     gene            complement(2774..3553)
                     /locus_tag="VFU20_02780"
     CDS             complement(2774..3553)
                     /locus_tag="VFU20_02780"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5285420.1"
                     /translation="MTIASGAAIGVDESGSGDALPILFLHGVGSDKSVWAPQLDHFGG
                     QRRAVALDYPGYGESDPAAPGTTRDDFAGAILAAMDALGIGQAHVCGLSLGGVVAIAI
                     HHAAPERCASLVLADTFAVHPDGKAIYERSVAASASMHELAAARTPVLLAPGASEDLH
                     RQVRETMARIDPETFRIGAEAVWLADQRDRAMKIGVPTLVLAGAEDTVTPPELSHDLA
                     DMIPDAELHTLAGAGHLPNVERPSDFNDAVDAFIRRAEARR"
     gene            3613..4152
                     /locus_tag="VFU20_02785"
     CDS             3613..4152
                     /locus_tag="VFU20_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5285421.1"
                     /translation="MTRMTVNGEAIHYLLDPRTPLLWALRDASNLTGTKHGCDSGDCG
                     ACTVIVDGRAVLSCSVSIASLEGSDVVTIEGLSAGRTHPVQQAWAAEQVSQCGYCEPG
                     FIMAIAALLQSSPSPSREAIDALPNICRCGAYPRIRKAIARAATAAAPAAPAAAPARP
                     DRLSNGSSAEEHVSPENRP"
     gene            4171..5127
                     /locus_tag="VFU20_02790"
     CDS             4171..5127
                     /locus_tag="VFU20_02790"
                     /inference="COORDINATES: protein motif:HMM:NF023204.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RcnB family protein"
                     /protein_id="HEU5285422.1"
                     /translation="MRNSLLFLLLANAVAVPALAADERDFRAARRDAARAERAEARAE
                     APEARSERTGARSQPSTRGDRPISMRSVEGGESDQPVLRSAVREPVAERRAPRAVEIE
                     SARPTLELRREPAPQRAERRGPRIVEVDSPRPALEQRTGTDTVREWRRAEREGARSPA
                     LVEQRTDIGWEPLRKRKEQSEGTDSAMRRAPFVSRVPREGTQPPTRAEARPARLTTSH
                     WRGDWRSDRRFDWRSHRRRHRSLFHFGFYYDPFGWSYRPYFIGWRLWPSYYRSSYWLH
                     DPWAYRLPYAPPGYRWIRYYDDALLVDTWDGTVVDVIRNFFW"
     gene            5226..>6136
                     /locus_tag="VFU20_02795"
     CDS             5226..>6136
                     /locus_tag="VFU20_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEU5285423.1"
                     /translation="MSLIRRLTFSASLIALAGCASVPPPAPVAPAAAEVGAPAPEAAR
                     KSPHDRLFDLFKASDEASLSRNPLSALFRGDLRYADRLGDGITDEYYAAERAAAEADL
                     AALRAIPRAELNATDQVAYDVFEYSTKDTLRGLQPDMLALTAVRPMNHFFGFHTFYPT
                     IASGKGAAPFKTLVDYENNLKRHRDFVVYLDRAIGRFRQGRDTGVVETKMTVRNMIEQ
                     LDNQLKEKPEDSAYFGPVKQFPAEIGAADRARLTGEYRSTIADGLYPALTRLRDFLKS
                     EYLPSARDGFGIMYMKGGDKLYRYLIES"
BASE COUNT          916 a         2205 c         2095 g          920 t
ORIGIN      
        1 ggcggtcggc cagctcggcg accagccgga gttgcgggcg atttccgccg cggtcgtcgc
       61 cgcgggcttg ctggcggcca accggcggct ggtccgaacc ggggtgcgga tgctgctcgc
      121 gcacgagatc gcgactctcg ccaaggatgt gatcaagcgg cgcatcgacc ggacccggcc
      181 gcacagcgcc cggaccgagg cggagcggac cgccaagccc gggcggcaca ccgccaagac
      241 caagaccagc tttccgtcgg gccacagcgc cggcgcgatc gccgtcgccc gcgccttggc
      301 gcgcgaatat cccggcttgc aggcgcccgc gctcgccgcc gccgggattg tcgccgccgc
      361 gcaggtcccc cgcctcaacc attacccgag cgacgtcgtg gccgggctgg cgctcggcgc
      421 tgcttcggaa gcggccgccg acctcgtcat gcggctcggc gagcggctcg aggacgcggc
      481 tacacccgaa gcgccgccgc agcctcggcc tcgcgcttga ggttgcgcgc cagctgcgcc
      541 tggccgttga gatattgcgg cggcacgtgg atgtcgcggt aatcgagcgc cgcggccgcg
      601 cgcagggtcc acatcgggtc gatcagatgc ggccggccga gcgcgaccag gtcggcgcgg
      661 ccggcggcga ggatcgaatt ggcatggtcg ggctcgtaga tgttgccgac cgccatggtc
      721 gcgacccgcg cctcgttgcg gagcttgtcc gaaaacggcg tctggaacat ccggccgtag
      781 accggctgcg cgccggtcca ggtctggccc gccgagacgt cgatcaagtc ggcgccggct
      841 tgcgcgaacg cctcggcgat cgcgaccgca tcgtccgggg tgatcccctg gtcgccggcc
      901 cagtcgctgg ccgagatgcg gaccgacatc ggcttgtgcg gcggccaggc ggcgcgcatc
      961 gccgcgaaca cctcgagcgg gtatctgagc cggttgtcga ggctgccgcc atagtcgtcg
     1021 gtgcgccggt tctgaagcgg agtgatgaag ctcgacagca ggtagccgtg cgcggcgtgg
     1081 agctcgacca tgtcgaagcc ggcttccagc cccatttgga ccgcggccac gaactgctcg
     1141 cgcaccatgt ccatgtcggc gcgggtcatt gccctgggcc gctggttggc ctccgaccag
     1201 ggcacgtcgg acgccgccat taccggccaa ttgccctcgt cgagcggcgc gtcattgcct
     1261 tcccagccga gccgggtcga gcccttgccg cccgaatggc cgagctgcag gcagatcctg
     1321 gccttgctgt tggaatggac gaaatcgacg atccgacgcc aggcggcgag atgctcgccg
     1381 ttccacatgc cggtgcagcc ggggctgatc cgcccttcgg gcgagacgca ggtcatctcg
     1441 gtgaacacga ggcccgcgcc gcccagcgcg cgctcggcca gatggacgaa atggaagtcg
     1501 cccggggttc cgtcgacggc cgagtacatc gccatcggcg agacggtgat tcggttctcg
     1561 acgctcatgt cgcgcaagcg gaacggagcg aacatcggcg ccgcggtctt gcccggaacc
     1621 ccggcgcgct cccagaacca gcgctcgacg ctttccagcc agtccttgtc gcgcagcctc
     1681 aggttctcat ggctgatgcg ctggcttcgg gtcagcagcg aataagcgaa ctgcagcggc
     1741 tcgaaatgca aatagcgctc gagagtctcg aaccattcgg tcgagttgcg cgcgctgttc
     1801 tgcagtttga gcacctcgag gctgcgctcc gcatgatatt cgaacagggc cgcgtcgagc
     1861 tcgaggcccg ggcggttgag cacgtcggcc agcttgatcg cgtcctcgag cgccagcttg
     1921 gttcccgagc cgatcgagaa atgcgcgctg tgcgcggcgt cgcccaggag gatgaagcgg
     1981 ccgtgcgacc aacgctcgca cttgattcgg cggaaattga gccacgcggc cgggcccggc
     2041 aaatgcgcgg cgttcgagac cagcggatgc ccgtcgagat attcggcgaa gatggtttcg
     2101 cacctggcga tcgcctcggc ctggtcgatg cggtcgaagc cgaggccgcg ccatacggct
     2161 tccgagcatt cgacgatgaa ggtcgagcag tcgggtgcga acttgtacgc gtgcgcccag
     2221 atccagccgg cgtcggtctc ctcgaacgcg aaggtgaagg cgtcgaacgc tttcgacgtt
     2281 ccgagccaga tgaacttgtt ggcgcggacg tcgacgtcga cctcgaacgc gtccgcgaag
     2341 cggtcgcgga cccggctgtt gatgccgtcc gaggcgatca ccaggtcgta ttcagcccat
     2401 ttgggatcgt cggggtcgca ttcgtggtcg aagctcagcc cgactcccag ctcggccgcg
     2461 cgctgctgca ggatttcgag cagccgcatc cggccgatgc cgatgaagcc gtggcccgac
     2521 gagcggatgc actgcccgcg gtaatggacg tcgatgtcgt cccaatgcgc gaactcgtcg
     2581 gcgatggccg tggccgactg ggggtcgttg gcggtcagat tctcgaccgt ctggtcggag
     2641 aagacgactc cccagccgaa ggtcaccccg cgcgggttgc gctcgaacac atggacctcg
     2701 tggccggggt cgcgaagctt catcgagatg gcgaaataga ggccggccgg gccgccgccg
     2761 atgcaggcga tcttcatcgc cgcgcctcgg cgcgccggat gaacgcatcg accgcatcgt
     2821 tgaagtcgct cggccgttcg acgttcggca ggtggcccgc gcccgcgagc gtgtgcagct
     2881 cggcgtccgg gatcatgtcg gcaagatcgt gcgacagctc gggcggggtg accgtgtcct
     2941 cggccccggc cagcaccagc gtcggcacgc cgatcttcat tgcccggtcc cgctggtcgg
     3001 ccagccagac ggcttccgcg ccgatccgga atgtttcggg gtcgatccgc gccatcgtct
     3061 cgcgcacttg ccggtgcaga tcctcggacg ctccgggcgc cagcagcacc ggcgtccgcg
     3121 ccgccgccag ctcgtgcatc gacgcgctcg ccgcgaccga gcgctcgtaa atcgcctttc
     3181 catccgggtg gaccgcgaac gtgtcggcga ggaccagcga cgcgcagcgt tccggcgccg
     3241 cgtggtggat cgcgatcgcg accactccgc cgagcgacag gccgcagaca tgcgcctggc
     3301 cgatgcccag cgcatccatc gcggccagga tcgcgcctgc gaaatcgtcg cgggtggttc
     3361 ccggcgccgc gggatcgctt tcgccgtagc cggggtagtc gagcgcaacc gcccggcgct
     3421 ggccgccgaa atggtcgagc tgcggcgccc acaccgattt gtcggacccg actccgtgga
     3481 ggaacaggat cggcagagca tcgccgcttc cggactcatc gacgccgatc gcggcgccgc
     3541 tcgctatggt cacccgcccc atgccgccat gatggcgttg gcgggcggct ttgcaagcgc
     3601 tacgggacgg ccatgacgcg catgacggtc aacggcgagg cgatccatta cctgctcgat
     3661 ccccggactc cgctgctgtg ggcgctgcgc gacgcctcca acctgaccgg caccaagcac
     3721 ggctgcgaca gcggcgactg cggcgcctgc acggtgatcg tcgacggccg cgcggtcctg
     3781 agctgcagcg tcagcatcgc ctcgctcgaa gggtcggatg tcgtcaccat cgagggattg
     3841 tcggcgggcc gcacccaccc ggtgcagcag gcctgggcgg ccgagcaggt cagccagtgc
     3901 ggctattgcg agcccggctt catcatggcc atcgcggcgc ttctgcagtc gagcccgagc
     3961 ccgagccgcg aggcgatcga cgcgcttccc aacatctgcc gctgcggcgc ctatccgcgc
     4021 atccgcaagg cgatcgcccg ggcggcgacg gccgcggctc cggcggcgcc ggcggcggcc
     4081 cccgcgcggc cggaccggtt aagcaacggc tcatctgccg aggagcacgt ttcgccggag
     4141 aatcgaccct gacgtgccgg gaccgcagcc atgcgtaaca gcctcctttt cctgctcctc
     4201 gccaatgccg tcgccgtgcc ggccctcgcc gcggacgagc gcgacttccg agccgcccgc
     4261 cgcgacgccg cccgagccga gcgcgccgag gctcgcgccg aggctcccga ggcccgctcc
     4321 gagcgcaccg gcgcgcggtc gcagccctcg acccggggcg accgcccgat ctcgatgcgc
     4381 tcggtggagg gcggcgagag cgaccagccc gttctgcgct cggcggttcg cgagccggtg
     4441 gccgagcgcc gggcgccccg cgccgtcgaa atcgaatcgg cccgcccgac gctcgagctg
     4501 cgccgcgagc ccgcgccgca gcgtgcggag cggcgcggcc cgcgcatcgt cgaggtcgat
     4561 tcgccccgcc cggcgctcga gcagcgcacc gggaccgaca cggtccgcga gtggcgccgg
     4621 gccgagcgcg agggcgcccg cagcccggcg ctggtcgagc agcgcaccga catcggctgg
     4681 gagccgcttc gcaagcgcaa ggagcagagc gaggggaccg actcggccat gcgccgggcc
     4741 cccttcgtca gccgcgtccc gcgcgagggc acccagcctc cgacccgggc cgaggcaagg
     4801 ccggcgcggc tgacgaccag ccactggcgc ggcgactggc ggtccgaccg ccgcttcgac
     4861 tggcgcagcc atcgccgccg gcaccgctcg ctgttccact tcggcttcta ttacgacccg
     4921 ttcggatgga gctaccggcc ctatttcatc ggctggcggc tgtggccgag ctattaccgg
     4981 agcagctact ggctccacga tccctgggcg taccggctgc cctacgcgcc cccgggctac
     5041 cgctggatcc gctactatga cgacgcgctg ctggtcgaca cgtgggacgg cacggtcgtc
     5101 gacgtgatcc gcaacttctt ctggtagaca gccccatcgc gttggtcgaa cgggcgttgc
     5161 ggtttccggc cgcgcgccct tcgggtggcg gcactcccga tcccatctct tgcaggtgct
     5221 cctccatgag cctgatccgc cgactgacct tctccgcatc gctgatcgca ctggccggct
     5281 gcgcgtcggt cccgccgccc gcgccggttg ccccggccgc ggccgaggtt ggggcgcccg
     5341 cgcccgaagc cgctcggaag agcccccacg accgcctgtt cgacctgttc aaggccagcg
     5401 acgaggcgag cctcagccgc aacccgctga gcgccctgtt ccgcggcgac ctgcgctatg
     5461 ccgatcggct cggcgacgga atcaccgacg aatattacgc ggccgagcgc gccgccgccg
     5521 aggcggacct cgccgccctg cgcgcgatcc cgagagccga gctcaacgcc accgaccagg
     5581 tcgcctacga cgtcttcgaa tattcgacca aggacacttt gcgcgggctc cagccggaca
     5641 tgctcgcgct gaccgcggtc cggccgatga accatttctt cggcttccac actttctatc
     5701 cgaccatcgc cagcggcaag ggcgcggcgc cgttcaagac gctggtcgac tatgagaaca
     5761 atttgaagcg ccaccgcgac ttcgtcgtct acctcgaccg cgcgatcggc cgcttccgcc
     5821 agggccgcga caccggcgtc gtcgagacca agatgacggt ccggaacatg atcgagcagc
     5881 tcgacaacca gctcaaggaa aagccggagg actcggccta cttcggcccg gtcaagcaat
     5941 tcccggccga gatcggcgcg gccgaccgcg cgcggctgac cggcgaatat cgctcgacga
     6001 tcgccgacgg gctctatccg gcgctgaccc ggctgcgcga tttcctcaag tcggaatatc
     6061 tgcccagcgc ccgcgacggc ttcgggatca tgtacatgaa gggcggcgac aagctctacc
     6121 gctacctgat cgaatc
//
LOCUS       DASTZO010000102         6441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_85538,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000102 DASTZO010000000
VERSION     DASTZO010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85538"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="VFU20_02800"
     CDS             <1..231
                     /locus_tag="VFU20_02800"
                     /inference="COORDINATES: protein motif:HMM:NF026098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase dimerization
                     domain-containing protein"
                     /protein_id="HEU5285424.1"
                     /translation="LAATIALETVLGTAWLAAAGREAMDSIVRRVASPNGTTEAGLAV
                     LDPHLDDLVASTIDAAARRGAELAEEAADRSS"
     gene            281..1171
                     /locus_tag="VFU20_02805"
     CDS             281..1171
                     /locus_tag="VFU20_02805"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEU5285425.1"
                     /translation="MAEQRTFDDRDGWIWFDGKLVPWREANVHILTHALHYASSVFEG
                     QRAYGGTIFKLTEHSQRLHKSARILGFEIPWSVEEVDAACIAVLEANKLVDAYMRPVA
                     WRGSEQMGVTARGTKPHLAIAAWEWGKYFDPKLAEKGIRLDIARYRRPAPYTAPVHSK
                     AAGLYMIATLSRQQAEGRGYDDALMLDWRGQVAEATGANVFFVRDGAIHTPTPDCFLD
                     GITRRTVMDLARNRGVEVVERAIWPEELESFEQMFLTGSAAEVTFVRSAGPWSFAVGD
                     LSRQLARDYDDTVNGRIPNR"
     gene            1235..1309
                     /locus_tag="VFU20_02810"
     tRNA            1235..1309
                     /locus_tag="VFU20_02810"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1267..1269,aa:Gln,seq:ttg)
     gene            1389..2543
                     /locus_tag="VFU20_02815"
     CDS             1389..2543
                     /locus_tag="VFU20_02815"
                     /inference="COORDINATES: protein motif:HMM:NF006488.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEU5285426.1"
                     /translation="MNPLYEQMRTSVFERMSALAARHGAVNLGQGFPDFGWPEAILDA
                     AARALTEGSNQYPPSRGLPPLRAAVAAHYNRHQGLAIDADHVCVTSGATEALGAAILA
                     TVRPGDEMIILTPAYDCYAPMIRRAGGTVREVALKPPGWRIERDGLAAAVGPNTRAIL
                     FNNPHNPAGRLFDEAELEAVAAVAREHDLTVISDEVWEHIALDGGRFTPLASLPGMAA
                     RTIKIGSAGKIFSLTGWKVGWMVAAPEMAAVAARAHQFLTFSTAPNLQSAVAFGLGEG
                     DSWIEPMKQRFERARDRMTQGLRDAGFAVLDAASTYFLCVDLAASGIALDDEGFAAVA
                     VEQAGVAVVPLSAFAEADPPRHLVRLCFGKKDQTIDAGVAAMAKAKELLA"
     gene            2540..4006
                     /locus_tag="VFU20_02820"
     CDS             2540..4006
                     /locus_tag="VFU20_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5285427.1"
                     /translation="MSPADEAAAILDRLGVDASAGELVSGSPIDGRPIGRVVTGDPAA
                     ACERASTAFLHWRSVPAPRRGELVRLIGEELRAAQEPLARLVTLETGKIVQEGLGEVR
                     EMIDICDFAVGLSRQLYGLTIASERPNHRMIEQWHPLGPVLVISAFNFPVAVWAWNAA
                     LALVCGDPVVWKPSEKTPLTAAATMALAERALQRFGDAPDGLLQLVQGGRDIGGALVD
                     DPRIALVSATGSTAMGRSVGPRVAQRFGRSLLELGGNNAMIVAPSADLELAGRAILFS
                     AAGTAGQRCTSLRRLIVHDSIADALVTRLRDLFARAAIGDPREPGTLIGPLIDEAAFD
                     AMAKVLGDKIERVEAVPGGFYVRPAIVEVPAQQGTVLEETFAPILYVLRYCAFDEAIA
                     LNNAVAQGLSSSIFTNDLREAERFIAAAGSDCGIANVNIGPSGAEIGGAFGGEKETGG
                     GRESGSDSWRAYMRRQTNTINYGSDLPLAQGIRFDVAP"
     gene            complement(3971..4819)
                     /locus_tag="VFU20_02825"
     CDS             complement(3971..4819)
                     /locus_tag="VFU20_02825"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5285428.1"
                     /translation="MTARPELALVEGFERALLNHRLHMVYQPKVRLRDGALTRVEALV
                     RWDDPQFGSVAPSRFVPLAEKHGLIEELTQWGLRTTLRQWLKWREEGIDTSIAFNISA
                     LSLEQLDFPDLVERMCRALDVPTDRLVLELTESATQPLIKLMDTLTRFRIKGIGLAID
                     DFGTGYSTLMQLRQLPFTEVKIDRFFVSDAVTSQESRLIVKTIVELAHGLGLTATAEG
                     VETREQLQSLAELGCDVAQGYLISQPLEPDALHSWKTEFRGRWPGLIEDEGLALWSYV
                     ESDPLR"
     gene            complement(4816..5205)
                     /locus_tag="VFU20_02830"
     CDS             complement(4816..5205)
                     /locus_tag="VFU20_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5285429.1"
                     /translation="MKQPRLLLVDDEPALADFIASAARECGFDATLTSDDEQFREEFR
                     AIRPDMVVLDLGMPGMDGIELLRFLAEERFQSPVLIISGFDRRVLESAFRLGEARGLT
                     MVGPLEKPVRLEALESLLTELKSTLVQ"
     gene            5348..6052
                     /locus_tag="VFU20_02835"
     CDS             5348..6052
                     /locus_tag="VFU20_02835"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5285430.1"
                     /translation="MFGSLIRKKPETVWNQDDATFESTTFTLSTEVPRPCERRTDARL
                     LPTLRVAKLVGHDCEQLIRVRNISAGGLMAETVQVPPNVGEHVTVELSSQTIPSSVVW
                     IREGTVGLKFDQNVDLGELLAGRKPRHGFRPRPPRLDVSCKASIRVGKVYYTVDVHDI
                     SLGGMKVEPIEEYCLGKDVVVVVESLRPVKGQVRWFADRRAGIVFDQELKFEELAEWV
                     GKRLELASLKASYQSK"
     gene            6113..>6441
                     /gene="msrA"
                     /locus_tag="VFU20_02840"
     CDS             6113..>6441
                     /gene="msrA"
                     /locus_tag="VFU20_02840"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU5285431.1"
                     /translation="MADEVAVLAGGCFWCTEAVFLDVAGVESVESGYTGGDTINPTYK
                     QVCGGDTGHAEAIRITFDPAQVRYEDLLDIFFATHDPTQLNRQGNDIGTQYRSAIFPQ
                     SPEQEAKA"
BASE COUNT         1014 a         2198 c         2259 g          970 t
ORIGIN      
        1 ctggcggcga cgatcgcgct cgaaacggtg ctcggcaccg cctggctggc cgccgccggg
       61 cgcgaggcca tggactcgat cgtgcgccgc gtcgccagcc ccaacgggac gacggaggcc
      121 gggctggcgg tgctcgaccc gcacctcgac gatctcgtcg catcgaccat cgacgccgcc
      181 gcccgccgcg gcgccgagct cgccgaggag gcggccgacc ggtcgagttg attccacccc
      241 ggcgctgcct taggccgctc gggttcaact gggacaatgg atggccgagc agcgcacttt
      301 cgacgaccgc gacgggtgga tctggttcga cggcaagctc gtcccgtggc gcgaggccaa
      361 cgtccacatc ctcacccacg cgctgcatta tgcgagcagc gtgttcgagg gccagcgcgc
      421 ctacggcggc acgatcttca agctcaccga gcacagccag cggctgcata agagcgcccg
      481 gatcctcggc ttcgagatcc cgtggagcgt cgaggaggtc gacgccgcgt gcatcgcggt
      541 gctcgaggcg aacaagctcg tcgacgccta catgcggccc gtcgcctggc gcgggtccga
      601 gcagatgggg gtcaccgccc gcggcaccaa gccgcacctc gccatcgccg cctgggaatg
      661 gggcaaatat ttcgacccca agctggccga gaagggcatc cggctcgaca tcgcccgcta
      721 ccgccggccc gcgccctaca ccgcgccggt gcattccaag gcggccggcc tgtacatgat
      781 cgcgactttg tcgcggcagc aggcggaagg gcgcggctac gacgacgcgc tgatgctcga
      841 ctggcgcggg caggtggcgg aggcgacggg cgccaacgtc ttcttcgtgc gcgacggcgc
      901 gatccacacg ccgacgcccg actgcttcct cgacgggatc acccggcgga cggtgatgga
      961 cctggcgcgc aaccgcgggg tcgaggtggt cgagcgggcg atctggcccg aggagctcga
     1021 gagcttcgag cagatgttcc tgaccggaag cgcggccgaa gtgaccttcg tccggtcggc
     1081 cggcccgtgg agcttcgccg tcggcgacct gtcccgccag ctcgcccggg actatgacga
     1141 cacggtcaac gggcgcattc ccaaccgcta ggcccttttc tcgtcccgtt ccggggtata
     1201 agcgccgcct ctcccgcaag acgggcccgc ctgctggggc gtcgccaagt ggtaaggcac
     1261 cggtttttgg taccggcatt cgcaggttcg aatcctgccg ccccagccaa tctccctgca
     1321 ccgaaagcta cgtctcgtcg gagcggcgcc cgcgcgctaa ggaccgcgaa gctggcgagg
     1381 agacgcgctt gaatccgctt tacgagcaga tgcgaaccag cgtgttcgag cgcatgtcgg
     1441 cgctcgccgc caggcacggc gcggtgaacc tggggcaggg cttccccgac ttcggctggc
     1501 ccgaagcgat cctcgacgcg gcggcgcggg cgctcaccga aggctcgaac cagtacccgc
     1561 cgtcgcgcgg tctgccgccg ctgcgagcgg cggtcgccgc gcactacaac cgccaccagg
     1621 gcctcgcgat cgacgccgat catgtctgcg tgaccagcgg cgcgaccgaa gcactcggag
     1681 cggcgatcct ggcgacggtc cggccgggcg acgaaatgat catcctgacc ccggcctacg
     1741 attgctacgc gccgatgatc cggcgggcgg gcgggacggt ccgcgaggtg gctctgaagc
     1801 cgccgggctg gcgcatcgag cgcgacgggc tcgcggcggc ggtcgggccg aacacccgcg
     1861 cgatcctgtt caacaacccg cacaacccgg ccgggcggct gttcgacgaa gcggagctgg
     1921 aagcggtcgc cgcggtcgcg cgcgagcacg acctgacggt gatcagcgac gaggtgtggg
     1981 agcatatcgc tctcgacggc gggcgcttca cgccgctcgc gtcgctgccg gggatggcgg
     2041 cacggacgat caagatcgga tcggccggca agatcttttc gctgaccggg tggaaggtcg
     2101 gctggatggt cgcggctccg gagatggcgg ccgtcgcggc gcgcgcgcac cagttcctga
     2161 ccttctcgac ggcgcccaat ctgcagtcgg cggtcgcgtt cgggctcggc gagggcgatt
     2221 cgtggatcga gccgatgaag cagcgcttcg agcgcgcccg cgaccggatg acgcaggggc
     2281 tgcgcgacgc cggattcgcg gtgctcgacg ccgcctcgac ctatttcctg tgcgtcgacc
     2341 tggcggcgtc ggggatcgct ctcgacgacg agggcttcgc ggccgtggcg gtcgagcaag
     2401 cgggggtcgc ggtggtgccg ctgtcggcgt tcgcggaggc cgatccgccg cggcatctgg
     2461 tccggctgtg cttcggcaag aaggaccaga cgatcgacgc cggggtcgcg gcgatggcca
     2521 aggcgaagga gctgctggca tgagcccggc cgacgaagcc gcggcgatcc tcgaccggct
     2581 gggagtcgac gcgtcggccg gcgagctggt cagcgggtcg ccgatcgacg gacggccgat
     2641 cgggcgggtg gtcaccggcg atcccgccgc cgcctgcgag cgggcgtcga ccgccttcct
     2701 gcactggcgc agcgttccgg cgccgcggcg cggcgagctg gtgcggctga tcggcgagga
     2761 gttgcgcgcg gcgcaggagc cgctcgcccg gctggtgacg ctcgagaccg gcaagatcgt
     2821 ccaggaaggc ctcggcgagg tccgggagat gatcgacatc tgcgacttcg cggtcggtct
     2881 gtcgcgccag ttgtacggcc tgaccatcgc cagcgagcgc ccgaaccacc ggatgatcga
     2941 gcaatggcac ccgctgggcc cggtcctggt gatcagcgcg ttcaatttcc cggtcgcggt
     3001 gtgggcgtgg aacgcggcgc tggcgctggt ctgcggcgac ccggtggtgt ggaagccgag
     3061 cgagaagacc ccgctgacgg ccgccgcgac gatggcgctg gcggagcgcg cgctgcagcg
     3121 gttcggggac gcgcccgacg ggctgttgca gctggtccag ggcgggcgcg acattggcgg
     3181 ggcgctggtc gacgacccgc gcatcgcttt ggtttcggcg accggctcga ccgcgatggg
     3241 gcgcagcgtc ggcccgcgag tcgcgcagcg gttcggccgc tcgctgctcg agctcggcgg
     3301 caacaatgcg atgatcgtcg cccccagcgc cgacctcgag ctcgccgggc gcgccatcct
     3361 gttctccgcc gcgggcacgg ccggccagcg ctgcacgtcg cttcgccggc tgatcgtcca
     3421 cgacagcatc gccgatgcgc tggtgacgcg cctgcgcgac ctgttcgccc gcgccgcgat
     3481 cggcgacccg cgcgagccgg gcacgctgat cgggccgctg atcgacgaag cggcgttcga
     3541 cgccatggcc aaggtgctcg gcgacaagat cgagcgggtc gaggcggtcc cgggcggatt
     3601 ctacgtgcgc cccgcgatcg tcgaggttcc ggcgcagcag ggcacggtcc tcgaggaaac
     3661 gttcgcgccg atcctctacg tgctgcgcta ttgtgcgttc gacgaggcga tcgcgctcaa
     3721 caatgcggtg gcccagggcc tgtcgtcgtc gatcttcacc aatgacctgc gcgaggccga
     3781 gcggttcatc gccgcggccg gatcggattg cgggatcgcc aacgtcaaca tcggcccgtc
     3841 gggggcggag atcggcggcg cgttcggcgg cgagaaggag accggcggcg ggcgcgagag
     3901 cggctccgac agctggcgcg cctacatgcg gcgccagacc aacaccatca actacggcag
     3961 cgacctgccg ctagcgcaag ggatcagatt cgacgtagct ccatagggcg aggccctcgt
     4021 cctcgatcag ccccggccag cgcccgcgga actccgtttt ccagctgtgc agcgcgtccg
     4081 gctcgagggg ctgcgagatc agatagccct gcgcgacgtc gcagccgagc tcggccagcg
     4141 actgcagctg ctcgcgggtc tcgacccctt ccgcggttgc ggtcaggccg agcccgtggg
     4201 cgagctcgac gatggtcttg acgatcagcc gcgattcctg ggacgtgacc gcgtcgctga
     4261 cgaagaagcg gtcgatcttg acctcggtga acggcagttg gcgaagctgc atcagggtcg
     4321 aatagccggt cccgaaatcg tcgatggcaa ggccgatgcc cttgatccgg aaccgggtca
     4381 gagtgtccat cagcttgatc agcggctggg tcgcgctctc ggtcagctcg agcaccagcc
     4441 ggtcggtcgg cacgtcgagc gcccggcaca tccgctcgac caggtcggga aaatcgagct
     4501 gctcgaggct cagcgccgag atgttgaagg cgatcgaggt gtcgatgcct tcctcgcgcc
     4561 acttgagcca ctggcgaagc gtggtccgaa gcccccactg ggtcagctcc tcgatgaggc
     4621 cgtgcttttc ggccagcggc acgaagcgcg acggagccac cgagccgaac tgcgggtcgt
     4681 cccagcggac cagcgcctcg acccgggtca gggcgccgtc gcgcaaccgg accttgggct
     4741 ggtagaccat gtgcagccgg tgattgagca gcgcccgctc gaagccctcg accagcgcca
     4801 actcgggacg agcggtcatt gcaccagcgt tgacttaagc tcggtcagga ggctttcgag
     4861 cgcttcgagc cggaccggct tctcgagcgg cccgaccatc gtcagcccgc gcgcctcgcc
     4921 gagccggaaa gcgctttcga gcacccggcg gtcgaagccg ctgatgatca gcaccggcga
     4981 ctggaagcgc tcctcggcaa ggaagcggag cagctcgatg ccgtccatgc ccggcattcc
     5041 aaggtcgagc acgaccatgt ccggccgaat cgctcggaac tcctcgcgga actgctcgtc
     5101 gtccgaggtc agcgtggcgt cgaagccgca ttcccgagcg gcgctggcga tgaagtcggc
     5161 gagagccggc tcgtcgtcga ccagcagcag ccggggctgc ttcatgcctg cacctcctcc
     5221 tcacccctcg tcgcgttgca ctgcgctctt tgggccgatc aggtcaaacg atgaaacggc
     5281 tttttaacta tgcgcgccat aaagtcccga attggtgcgg tttcctcgcg ccttggagac
     5341 cattcgaatg ttcggatcgc tcattcgaaa gaagccggaa acggtctgga accaggacga
     5401 cgccaccttc gaatcgacga ctttcacttt gtcgacggag gtgccgcggc cgtgcgagcg
     5461 ccgcaccgat gcgcggctgt tgccgacgct ccgggtcgcg aagctggtcg gccacgactg
     5521 cgagcaactg atccgggtgc gcaatatctc cgccggcggg ctgatggcgg agaccgtgca
     5581 ggtgccgccc aacgtcggcg agcatgtcac cgtcgagctg tcctcgcaga ccatcccgtc
     5641 gagcgtcgtc tggattcgcg agggcaccgt cggcctcaag ttcgaccaga acgtcgatct
     5701 cggcgagctg ctcgccgggc gcaagccgcg ccacggcttc cgcccgcgcc cgccgcggct
     5761 ggacgtcagc tgcaaggcct cgatccgcgt cggcaaggtc tattacacgg tcgacgtgca
     5821 cgacatttcc ctgggcggga tgaaggtcga gccgatcgag gaatattgcc tcggcaagga
     5881 cgtcgtcgtc gtcgtcgaga gccttcggcc ggtcaagggc caggtccgct ggttcgccga
     5941 ccgccgcgcc ggaatcgtgt tcgaccagga gctcaagttc gaagagctcg ccgaatgggt
     6001 cgggaaacgg ctcgaactcg ccagcctcaa ggcgagctac cagtccaaat aggcctttaa
     6061 gggccactgc cgccgcacta tctgccgtgc ttcccgggaa aggagcaggc aaatggcgga
     6121 cgaagtcgcg gttcttgccg gcggatgctt ctggtgcacc gaggcggtgt tcctcgacgt
     6181 cgccggagtg gaatcggtcg aaagcggcta caccggcggc gacacgatca acccgaccta
     6241 caagcaggtg tgcggcggcg acaccggcca cgccgaggcg atccggatca ccttcgaccc
     6301 cgcgcaggtc cgctacgagg acctgctcga catcttcttc gccacccacg acccgaccca
     6361 gctcaaccgc caaggcaacg acatcggcac ccagtaccgc tcggcgatct tcccgcagtc
     6421 gccggagcag gaagcgaaag c
//
LOCUS       DASTZO010000103         5530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_764481,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000103 DASTZO010000000
VERSION     DASTZO010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5530
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_764481"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VFU20_02845"
     CDS             <1..818
                     /locus_tag="VFU20_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEU5285432.1"
                     /translation="HHPVGTCKLSWILRTPRNVALTDVMKRDQTLIPVTMDQEEVALR
                     FQKYALISAAVVDDAGRLVGQITVDDVVHIIQEEAGEDVMLLSGAGEGDINEPIREAY
                     VARVRWLVANLVTASLASFIISRFEDVIAQLVTLAVLMPIVAGVGGNAGTQTLAVTVR
                     AIATNQLTGSNRWRAVGRELRVAILNGITVAALIGAGVTLVLGSQALGAVIAVAMLFN
                     IIVAGLAGVLVPLALERAGTDPAVASSVFVTMVTDSMGFLLFLGLATAAGLVG"
     gene            859..1260
                     /locus_tag="VFU20_02850"
     CDS             859..1260
                     /locus_tag="VFU20_02850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008070210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 family protein"
                     /protein_id="HEU5285433.1"
                     /translation="MPRLHLTKVAFGCRTIEALEKRIAGRDVRGEVRVVTRMRPRRAD
                     ELIGGSLYWIVRHRLVACQQILRFEDRPDGRIDIVCSADLVIISAQPKRGHQGWRYME
                     ESSAPPAEGDGSGVGELPPHLYGRLAALALV"
     gene            1376..2122
                     /locus_tag="VFU20_02855"
     CDS             1376..2122
                     /locus_tag="VFU20_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285434.1"
                     /translation="MVQYLQEILADSWALSAVVFVGVFVLLAMVGIHLLSAPPPRESG
                     RNVRSTLAGTSARAVFHRDVPVGIRPVQQPPAKSVERATSVSFDPRIVLRNIYRALSA
                     LIVGFLLVGTVFTYLAATPANGLLLISGILAVLTLITFLQVLNFDRKMERRADPADRN
                     DLMRTISDDLAEKIEAGLRGDVPVRWHVGAPEVYEIGEEDFARARAMLDEGMQIDEIC
                     RDLDGEYAGWSEPRQRAYQGLIRTALDHRL"
     gene            2185..2862
                     /locus_tag="VFU20_02860"
     CDS             2185..2862
                     /locus_tag="VFU20_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HEU5285435.1"
                     /translation="MTQFSVSAAIDSKVAARAMLSHPFYRAWTEGRLPLDTLRGYARQ
                     YFHHVEAFPRAVSAVHSACPDRDGRRMLAENLAEEEGIEAGKQDHASLWLMFACGLGE
                     SEDAVREQRLNPETEALIETFRRLSRRSYASGLGALYAYESQFPGVASAKIEGLVERY
                     GIDDEPTLRFFRVHESADVEHSAVCRELLDRLPEDQRSEAVAAGEELAGALWNFLSGV
                     ESRATVQ"
     gene            complement(2863..3573)
                     /locus_tag="VFU20_02865"
     CDS             complement(2863..3573)
                     /locus_tag="VFU20_02865"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HEU5285436.1"
                     /translation="MRTLLPFGLPALAVLACTAAPAGAATRNFGVSGFDRIRVDGPYK
                     VRLATGVAPFAVATGGSAAALDSVAIDVQGRTLVVRTNRSTWGGYPGEARGPVEISIG
                     THDLNAAWLNGSGTLAIDKARGLSFDLSVQGAGSASIGSVAVDQLKVSISGTGSASVA
                     GAAPRLTAIVRGISTLDAAGLVARDATIGAQGPATVRLTVTNSAKIDAQGAATVELAG
                     RPGCTVKAAGSASVSGCR"
     gene            complement(3625..4350)
                     /locus_tag="VFU20_02870"
     CDS             complement(3625..4350)
                     /locus_tag="VFU20_02870"
                     /inference="COORDINATES: protein motif:HMM:NF022441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HEU5285437.1"
                     /translation="MQKAILAGMVAAAAASSGCAKDRQESAGPATTRNYPVGAFDRIE
                     VAGPYDVTVATGSAPSVRASGGERAIERMVVEVKGGTLRIHPRKRSGLHFGWSKSHPV
                     KLTVTVPRLAAAEIAGSGDISVDKVAGDSFEAGVAGSGNVRLGEVNVKRLKAGIAGSG
                     EISAGRGRALAADYEIAGSGDIDGAGVIAETAAVSIAGSGNVAAHATGTASVEIAGSG
                     DVRVTGGAKCSVSKAGSGNVQCS"
     gene            complement(4549..5085)
                     /locus_tag="VFU20_02875"
     CDS             complement(4549..5085)
                     /locus_tag="VFU20_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HEU5285438.1"
                     /translation="MASKALSFDVGDYVVYPKHGVGRVVELQSTEIAGIRLDLYVLRF
                     EKERMTLRVPVNKADAVGMRKLSSDKTMKDALETLKGKPKVKRTMWSRRAQEYEAKIN
                     SGDLTSIAEVTRDLFRADDQPEQSYSERQIFEAASSRLARELAAMEQTDEKAALAKIL
                     EILNEAAAIHNKSKELAA"
     gene            complement(5246..>5530)
                     /locus_tag="VFU20_02880"
     CDS             complement(5246..>5530)
                     /locus_tag="VFU20_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU5285439.1"
                     /translation="ELERIAGYAWARIERAAATGRTVTLKVKFADFELITRSKSFAAP
                     IPDLAGFAAAGQALLAGLHPLPKGIRLLGLGLHNLVDPEIEPPLQLGLAI"
BASE COUNT          826 a         1899 c         1869 g          936 t
ORIGIN      
        1 ggcatcaccc ggtcggcacc tgcaagctgt cgtggatctt gcgcaccccg cgcaacgtcg
       61 cgctgaccga cgtgatgaag cgcgaccaga cgctgatccc ggtgacgatg gaccaggagg
      121 aagtcgcgct gcgcttccag aaatacgcgc tgatctcagc cgctgtggtg gacgacgccg
      181 gacggctggt cggccagatc accgtcgacg acgtcgtcca catcatccag gaggaagccg
      241 gcgaggacgt gatgcttctc tccggcgcgg gcgaaggtga catcaacgag ccgatccgcg
      301 aggcttacgt ggcccgggtg cgctggctgg tcgcaaacct cgtcaccgcc agcctcgctt
      361 cgttcatcat ctcgcggttc gaggacgtga tcgcgcagct ggtgacgctt gcggtgctga
      421 tgccgatcgt cgccggggtc ggcggcaacg ccggcaccca gaccctggcc gtcaccgtgc
      481 gcgcgatcgc caccaaccag ctgaccgggt ccaaccgctg gcgggcggtc ggccgcgagc
      541 tgcgggtcgc gatcctcaac ggaatcacgg tcgcggcgct gatcggggcc ggggtgacgc
      601 tggtgctcgg aagccaggcg ctgggagcgg tgatcgccgt cgcgatgctg ttcaacatca
      661 tcgttgccgg gctggccgga gtgctggtgc cgctggcgct cgagcgggcc gggaccgacc
      721 cggcggtcgc cagcagcgtg ttcgtgacca tggtaaccga ttcgatgggc ttcctgttgt
      781 tcctcgggct tgcgacggcg gccgggctgg tcggctaggc gattgccatc gccggcctga
      841 gctcccattt ccaggggcgt gcccaggctc catctgacca aggtcgcgtt cggctgccgg
      901 acgatcgagg cgctcgagaa gcggatcgcc ggccgggacg tgcgcggcga ggtgcgggtc
      961 gtcacccgga tgcgccccag gcgcgccgac gagctcatcg gcggctcgct ctactggatc
     1021 gtccggcacc ggctggtcgc ctgccagcaa atcctgcgct tcgaggaccg ccccgacggc
     1081 cggattgaca tcgtctgctc ggccgatctg gtcatcatct cggcccagcc caagcgcggc
     1141 caccagggct ggcgctacat ggaagaatcg agcgccccgc cggccgaggg cgacggctcg
     1201 ggcgtcggcg agctcccgcc gcacctctac ggccggctgg cggcgctggc gctggtctga
     1261 ctgcgccggc gcgggaccaa gtttttcggc cgcgccggcc gatgttttcc agcagcgacg
     1321 cccgtgttac tcttggccgg agcccattgt tcgccgatga ggcctgagaa gtccgatggt
     1381 gcaatatctg caggagatcc tcgccgattc ctgggctctt tcggccgtcg tcttcgtggg
     1441 ggtcttcgtg cttttggcga tggtcgggat ccacctcctc tccgccccgc ctccgcgcga
     1501 gtccggccgg aatgtccggt cgaccctcgc cggcacctcc gcccgggccg tgttccatcg
     1561 cgacgtgccc gtcggcatcc ggccggtgca gcagccgccg gcgaaaagcg tcgaacgggc
     1621 cacgtcggtt tcattcgatc cgcgaatcgt gctcaggaat atctaccgcg cactgtcggc
     1681 cttgatcgtc gggttcctgc ttgtcggcac cgtctttact tatctggcgg cgactccggc
     1741 caatgggctg ttattgatca gcgggatcct cgcggtgctg acgttgatca ccttccttca
     1801 ggttctcaat ttcgatcgca agatggaacg acgagccgat ccggcggatc ggaacgattt
     1861 gatgcgcacg atttcggacg atctcgccga gaagatcgag gccgggctgc gcggcgatgt
     1921 ccctgtccgg tggcacgtcg gcgctccgga ggtctacgag atcggcgagg aggacttcgc
     1981 tcgagcgcgg gcaatgctgg acgaagggat gcagatcgac gagatttgcc gagaccttga
     2041 cggtgagtac gccggatgga gcgagccccg ccaacgggcc taccagggat tgattcggac
     2101 ggctctcgac catcgtctct gacttgtcgc gccggggcgc gcgagttatc gccgccgttc
     2161 aatccctatc ccaggaggca tggcatgacc cagttctcgg tttcggcggc aatcgattcg
     2221 aaggtcgctg cccgcgcgat gctcagccat cccttctacc gggcgtggac cgaggggcgg
     2281 ctgccgctcg acacactgcg cggctacgct cggcaatatt tccaccatgt cgaggcgttc
     2341 ccgcgcgcgg tgagcgcggt gcacagcgcc tgccccgacc gcgacggccg gcggatgctg
     2401 gccgagaacc ttgccgagga agaggggatc gaggccggca agcaggacca tgcgagcctg
     2461 tggctgatgt tcgcctgcgg cctgggcgaa agcgaggatg cggtccgcga gcagcggctc
     2521 aaccccgaga ccgaggcgct gatcgagacc ttccgtcgcc tgtcgcgccg ctcctacgcg
     2581 tccggcctcg gagccctcta cgcctacgaa agccagttcc ccggggtcgc cagcgccaag
     2641 atcgagggcc tggtcgagcg ctacgggatc gacgacgagc cgacgctgcg cttcttccgg
     2701 gtccacgaga gtgccgacgt cgagcacagc gccgtctgcc gcgaattgct cgaccgcctg
     2761 cccgaggatc agcgctccga agcggtcgcc gccggcgagg agctggccgg cgcgctgtgg
     2821 aatttcctgt cgggggtcga gtctcgcgcg accgttcagt agctagcggc agccgctgac
     2881 gctcgccgat ccggccgcct tgacggtgca tcccggccgt ccggcgagct cgaccgtcgc
     2941 cgcgccctga gcgtcgatct tcgcgctgtt ggtgaccgtc agtctgaccg tcgccggccc
     3001 ctgcgcgccg atggtcgcgt ccctggcaac cagtcccgcc gcgtcgaggg tcgaaatgcc
     3061 gcggacgatc gcggtcagcc ggggcgcggc gccggcgacg ctcgcactgc cggtgccgga
     3121 aatcgagacc ttcagctggt cgaccgcgac cgagccgatg ctcgccgagc ccgcgccctg
     3181 gaccgacagg tcgaacgaca ggccgcgggc cttgtcgatc gccagggtgc ccgagccgtt
     3241 gagccaggcg gcattgagat cgtgggtgcc gatgctgatc tcgaccggcc ccctggcctc
     3301 gcccggatag ccgccccacg tcgagcggtt ggtgcgcacc accagcgtcc gcccctggac
     3361 gtcgatggcg accgaatcga gcgccgcggc cgagccgccg gtcgcgaccg cgaacggcgc
     3421 gaccccggtc gccaggcgga ccttgtacgg gccgtcgacg cggatccggt cgaagccgct
     3481 gactccgaag ttgcgggtcg ccgcgccggc cggggcggcg gtgcaggcga ggacggcgag
     3541 ggcgggcagg ccaaaaggca ggagggtgcg catggcgcgc accttcaccg cctcatggtt
     3601 aagatcgcct ttaagcgcgg ccgcctacga gcattggacg ttgcccgagc ccgccttgct
     3661 gacgctgcac ttggcgccgc cggtcacgcg cacgtcgccc gatccggcga tttccacgga
     3721 cgccgttccc gtcgcgtggg cggcgacatt gcccgatccg gcgatcgaca cggccgcggt
     3781 ctcggcgatc acgcccgcgc cgtcgatgtc gcccgatccg gcgatctcat agtcggcggc
     3841 cagcgcccgg ccgcggccgg cgctgatctc gcccgagccg gcgatgcccg ccttcagccg
     3901 cttgacgttg acctcgccca gccggacatt gcccgagccg gcgactccgg cctcgaaaga
     3961 atcgcccgcg accttgtcga cggaaatgtc gcccgagccg gcaatttccg cggccgccag
     4021 gcgcggcacg gtcacggtga gcttcactgg gtggctcttc gaccagccga aatgcagccc
     4081 cgagcgcttc ctggggtgga tcctgagagt cccgcccttg acctcgacca ccatccgctc
     4141 gatcgcccgc tcgccgccgc tggcgcggac ggacggggcg ctgccggtgg cgacggtcac
     4201 gtcatagggg ccggcgacct cgatccggtc gaacgccccg accgggtagt tgcgggtggt
     4261 cgccggccca gccgattcct ggcggtcctt ggcgcagccc gacgaggccg ccgcggcggc
     4321 gaccatccct gcgagtatcg ccttttgcat gtgatcctcc ctgatgctgt attagaacag
     4381 caatacacca actcggggag ggtcggcaag gcaaaagaaa cggacgccat gccgcttccg
     4441 gcccagtgag gacatgggga cgggtcgtgc ggggcatcca gtcacaaaga aaagggcgcg
     4501 ccccgcgaag gagcgcgccc ttaacctgcc cccttggacc gcgcggcctt aggccgcgag
     4561 ctccttggac ttgttgtgga tcgcggccgc ctcgttcaga atctcgagga tcttggccag
     4621 cgccgctttc tcgtcggtct gctccatcgc cgccagctcg cgggccaggc gcgacgaggc
     4681 ggcctcgaag atctgccgct ccgaatagct ctgctcgggc tggtcgtcgg cgcggaacag
     4741 gtcgcgggtc acctcggcga tcgacgtcag gtcgcccgag ttgatcttcg cctcatattc
     4801 ctgggcccgg cgcgaccaca tggtgcgctt gaccttgggc ttgcccttca gtgtctcgag
     4861 cgcgtccttc atggtcttgt cggacgacag cttgcgcatc ccgaccgcat ccgccttgtt
     4921 gacgggcacg cgaagggtca tgcgctcctt ctcgaagcgc agcacataga ggtcgagacg
     4981 aattccggcg atctcagtgc tctgaagctc gaccacccgg ccaactccat gcttggggta
     5041 aacgacataa tcgccaacat cgaagctcag cgctttggaa gccattattt tcctttccac
     5101 ttacggcgcc aagagccgac acgtttcgca tcgtccccca aaacgaccaa cggtcgcggc
     5161 tctgtcgcct ggtaacgttc ctgctagttc cggccgctgc ggaatcgcgg cgaccttcgc
     5221 gggcgatttt atagcatagg tttggctaaa tcgccagccc aagctgcagc ggcggctcga
     5281 tctcggggtc gacgagattg tgcagcccga ggccgagcag ccttatgccc ttgggcagcg
     5341 gatggagtcc ggcaagcagc gcctggccgg ccgcggcgaa gcccgcaagg tcgggaatcg
     5401 gcgcggcaaa gctcttcgag cgggtgatca gctcgaagtc ggcgaacttg accttcaggg
     5461 tcaccgtccg cccggtcgcc gccgcccgct cgattcgggc ccaggcatag ccggcgatcc
     5521 gctcgagctc
//
LOCUS       DASTZO010000104        10448 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_869893,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000104 DASTZO010000000
VERSION     DASTZO010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 10448)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10448)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10448
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869893"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..564
                     /gene="rdgB"
                     /locus_tag="VFU20_02885"
     CDS             <1..564
                     /gene="rdgB"
                     /locus_tag="VFU20_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HEU5285440.1"
                     /translation="GKLREIAELIEPLGVACVGAEELGLPEPEEIGNTFLDNADLKAR
                     EAADLSGLPALADDSGLAVDALHGLPGIFSARWAEDEQGNRDWMRAMERVWSRLEETG
                     PDASRDAHFACALSLAWPNDGQIEGFEGRVDGTLVWPPRGDKGFGYDPMFVPAGHQHT
                     FGEMDPAEKHKISHRAQAFRKLVAALK"
     gene            684..1808
                     /gene="hemW"
                     /locus_tag="VFU20_02890"
     CDS             684..1808
                     /gene="hemW"
                     /locus_tag="VFU20_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HEU5285441.1"
                     /translation="MALYVHWPFCVSKCPYCDFNSHVRAAVDQAEWRAALLADLAYEA
                     RLLAGRRLTSIFFGGGTPSLMEPATVAALIEAALDHWPAADGLEITLEANPNSVEAAR
                     FADLAAAGVNRLSLGLQSFDDDRLAFLGRAHSAREGLEALATAQRHFRRVSFDLIYAL
                     PGDSEAWWSTALDRALALGTGHLSLYQLTIEPGTRFAALAAAGKLEALDADRAAALYE
                     LTAERTAAAGLPAYEISNHARPGEESRHNLAYWRYGDYAGVGPGAHGRRLARRTVRHR
                     KPENFLSALARDGHGIAEDEPLAATQAADEALVMGLRLAEGIDAGSIAERFGLDSIVD
                     WTAVDRLARSGHVERDGGRIAATPAGRLVLDRVLAEIAVG"
     gene            complement(1814..2134)
                     /locus_tag="VFU20_02895"
     CDS             complement(1814..2134)
                     /locus_tag="VFU20_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285442.1"
                     /translation="MFTAFTSIILAALLQSAAINGQRDKYEACLTQAFESAKTQKMPA
                     EALDAHLRQACTSIEESFEGALIAFDLKNKVPRKQAAADAQLQVNDLVDNLVERYRAM
                     AARQ"
     gene            2271..3107
                     /gene="rsmI"
                     /locus_tag="VFU20_02900"
     CDS             2271..3107
                     /gene="rsmI"
                     /locus_tag="VFU20_02900"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU5285443.1"
                     /translation="MDEPLPPGLYVVATPIGNLADLSPRAAETLRRADRILAEDKRVS
                     AKLLAHAGARAPMATYHDHTSEAERAAIVARLGTEAVALISDAGTPLISDPGYKLVRA
                     ARAAGHPVHTVPGPCAAVAALTLAGLPTDRFLFTGFLPPKEKARNDAIGEIAGIRATL
                     ILYETGPRLAGTLAALAQGLGAREAAVAREISKLHEECVTGTLAELAERYAGAARRGE
                     IVIVVGPPAAGEGPSESDLDAALGEALARLSPSRAAAEVAARLGVPRKRAYARALQLA
                     AQ"
     gene            3104..3454
                     /locus_tag="VFU20_02905"
     CDS             3104..3454
                     /locus_tag="VFU20_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU5285444.1"
                     /translation="MNRYAAEKRGRGAETLAALWLRLHGWRIVARRARVPGGEVDIVA
                     RRGRTLAFVEVKARATEDAAVFSLDAYRLRRVAVAAERLAPRYMRDGDELRIDAIFIV
                     PRRWPRHLADIWQG"
     gene            3498..4469
                     /gene="gshB"
                     /locus_tag="VFU20_02910"
     CDS             3498..4469
                     /gene="gshB"
                     /locus_tag="VFU20_02910"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HEU5285445.1"
                     /translation="MPLRVAVQMDPLEAINIAADSTFAIMLKAQEIGHSLHHYTPGDL
                     SYAQGRLWTMARPVTVQPVAGDHYRFGEPEILDLGRDVDVVLMRQDPPFDLGYITATH
                     LLERIQGETLVVNDPASVRNAPEKLWVLDFAQFMPPTLVTRSLGLTRKFLAEHGEIVV
                     KPLHGFAGGSIFRIGRDAQNLASLIELFNGAYREPHVVQAFLPEIAEGDKRIVLIDGE
                     VAGAINRLPGEGEIRSNLAVGGKPAKTQLTAREEEICAALAPEVRKRGLLFVGLDVIG
                     GKWLTEINVTSPTGIVAIDALNGSDTPAMFWAAVERRLGERGRGRTG"
     gene            4480..5106
                     /locus_tag="VFU20_02915"
     CDS             4480..5106
                     /locus_tag="VFU20_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HEU5285446.1"
                     /translation="MSDWVIRLIEQSGYLGVAFLMFLETIFPPIPSEVIMPIAGMAAA
                     KGKLGFIPVVASGTAGAMLGNTVWYLAARALGVHRLKPLITRYGRWLTISWPEVARAE
                     RWFAQHGTFFVFFGRLLPTVRSLVSVPAGLLRMRFKSFFAASLLGTAGWTTILAGAGY
                     KLQDNVTEVERMVGPVSNAVLVVLAAGYVWRLWTHRHQDDRREESDLD"
     gene            complement(5267..6208)
                     /locus_tag="VFU20_02920"
     CDS             complement(5267..6208)
                     /locus_tag="VFU20_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEU5285447.1"
                     /translation="MADRPAPEELTEAQPARALAERWGNYLQHDRRRSSHTVRAYVAT
                     AHRFVDFLGRHRGEQIGRFALLALNASDLRAFLADRRASGLGASSAARELSAVRAFLN
                     YAAEQQGETAQLPRTRAPKRPRTLPRPASPEDAMGLAHDAAGAASVEWIGARDLAILL
                     LLYGAGLRVAEALSLTARVLPVGAAIRVIGKRSKSRVVPIVPAVAQAIEDYVRQCPFQ
                     LTGDATLFVGARGGPLNGDLVRRSVRSARQRLGLPDSLTPHALRHSFATHLLARGADL
                     RALQELLGHASLSSTQIYTAVDAARLLDVYRHAHPRA"
     gene            complement(6178..6753)
                     /locus_tag="VFU20_02925"
     CDS             complement(6178..6753)
                     /locus_tag="VFU20_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HEU5285448.1"
                     /translation="MGRVIQFEERAVAHLRERLGVIEEANQDLIAFARGHSGAVSAIH
                     AAVIAALEADSIEALLHVVTQEWPLILGIDAVALSLIVGDQGFRADGNGVQAVDPQIL
                     RRAIEQVDGVEMRTVARGHPLFGPACDLIRAEALIRIDCEPPLPCGLIALGQRTTQNL
                     DARHGSELLMFLGRSLGAMLRRWLTDPLQKN"
     gene            complement(6822..8453)
                     /locus_tag="VFU20_02930"
     CDS             complement(6822..8453)
                     /locus_tag="VFU20_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285449.1"
                     /translation="MMISLLLTSAAALALSPGEQHDHSPKLGKLAFETSCSAPADAEF
                     ELGLRWLHSFEYDRAEKQFLKAADTDPGCAIAHWGVAMANYHPLWAPPTAAELDKGKA
                     ALAKARAAGAKSKREQDYVAAIGAFYDHSERLDHKARALAYNSAMAQLRERYPDDDEA
                     AVFYALSQVAAGTLDKDPNFEREKSAAAILNQVLKEEPDHPGVAHYLIHSFDYPPLAE
                     LALPAARRYASIAPASAHAQHMPSHIFTRLGLWDEAIRSNRAAQAAAMAHARASGMAG
                     TWDQQLHAMDYLAYAYLQTGRDVDAQRVLDELQAIKQATSTPTTAYAVSAVPARVLLE
                     RRQWKEAAGFDLPADLGGLKALTDNKWAIANIRFANAVGAARSGDPALARAQAARLAE
                     LEQSLVTAPGAYDWKTQVSIERQIAEAWLAFAEGRKEDSVRLMRSAADLDDATEKHPV
                     TPGAILPAREQLGELLLELGRPAEAQAEFEASLKRAPRRLAGLYGAARSAKLAGNAAA
                     ARRHYGELVKLTQASDGGRAEVQEARSFVAELAGR"
     gene            complement(8472..9125)
                     /gene="fsa"
                     /locus_tag="VFU20_02935"
     CDS             complement(8472..9125)
                     /gene="fsa"
                     /locus_tag="VFU20_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU5285450.1"
                     /translation="MKFFADTAEIDEIRELAATGLLDGVTTNPSLVHKSGRDFIEVLR
                     EISKICPGPVSAEVVALDYDSMIREAEVLRKVAKNIAIKVPLTPDGLKACKALTGEGT
                     MVNVTLCFSAAQALLAAKAGASFISPFVGRHDDVGFDGMQLVADIRMIYDNYDFGTQI
                     LVASVRHPVHVIEAAKIGADVITAPPKIIHQLFKHPLTDAGIASFLKDWQASGQKIG"
     gene            9213..9989
                     /locus_tag="VFU20_02940"
     CDS             9213..9989
                     /locus_tag="VFU20_02940"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEU5285451.1"
                     /translation="MSEQVRIESGGGVLAITLARPERRNAITVAMYEALADAIGKAQD
                     DPSLRLITLRGEGEDFTAGNDLADFLAAMPREGGEEIAVWRLLRALAANDLPLVAAVH
                     GNAIGIGTTLLLHCDVVLAEDGTRFSLPFVDLGLVPEAASSLLLPRLAGRRRAARYLL
                     LAEPFGAEEASEWGLVTRIVPKGELDGALADTVAALLAKPAEALRLTQGLLRHGGKEE
                     LLERFELENRHFSERLQSAEVKEAITAFFEKRKPDFTKLG"
     gene            complement(10011..10397)
                     /locus_tag="VFU20_02945"
     CDS             complement(10011..10397)
                     /locus_tag="VFU20_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HEU5285452.1"
                     /translation="MSRVTFLRRNERGAAAIEFALAVPILVLFIYGIFRLGLLFQANA
                     GMQHALGEGARYATIYPTPTNSQIQTRMTSKLFGNGYGSFTVAAPVDGTGFKTLTVTY
                     TTPMDFLLVPGPTVTLTRSKKVYIAI"
BASE COUNT         1558 a         3656 c         3621 g         1613 t
ORIGIN      
        1 ggcaaattgc gcgagatcgc cgagctgatc gagccgctcg gggtcgcctg cgtcggcgcc
       61 gaggagctcg gcctccccga gcccgaggag atcggcaaca ccttcctcga caatgccgat
      121 ctgaaggcgc gcgaagcggc cgacctgtcg ggattgccgg cgctggccga tgacagcggc
      181 cttgccgtcg atgcgctgca cgggctgccc ggaatcttct cggcgcgctg ggccgaggac
      241 gagcagggca accgcgactg gatgcgggcg atggagcggg tgtggagccg gctcgaggag
      301 accgggccgg acgcgagccg cgacgcccat ttcgcctgcg ctctgtcgct cgcctggccc
      361 aacgacgggc agatcgaggg cttcgagggc cgcgtcgacg ggacactcgt ctggccgccg
      421 cggggcgaca agggcttcgg ctacgacccg atgttcgtgc ccgccggcca ccagcacacc
      481 ttcggcgaga tggacccggc cgaaaagcac aagatcagcc accgcgccca ggcgttccgc
      541 aagctggtcg ccgctctcaa atgaatgctc ccctctcctt caggggaggg gctgggggtg
      601 tcggcgagcg gccgcgagcc caagaactcc cccacccctc cgccgctttg cggctccaac
      661 tctcccctgc aggggagggg aaattggccc tctacgtcca ctggccgttc tgcgtcagca
      721 aatgccccta ttgcgacttc aacagccatg tccgagcggc ggtggaccag gccgagtggc
      781 gggccgcgct gctcgccgac ctcgcctatg aggcgcgcct gcttgccggc cgccggctga
      841 cctcgatctt cttcggtggc ggcaccccgt cgctgatgga gcccgcgacc gtcgccgcct
      901 tgatcgaggc ggcgctcgac cattggccgg cggcggacgg cctcgagatc acgctcgagg
      961 ccaaccccaa ttcggtcgag gcggcccgct tcgccgatct cgccgcggcg ggagtcaacc
     1021 gcctgtcgct ggggctgcag agcttcgacg acgaccgcct cgccttcctc ggccgagccc
     1081 attcggcccg cgaggggctc gaggcgctgg ccaccgcgca gcggcatttc cggcgggtca
     1141 gcttcgacct catctacgct ctgcccggcg acagcgaggc ttggtggagc acggctctcg
     1201 accgcgctct ggcgctcggc accggccatc tgtcgctcta ccagctgacc atcgagccgg
     1261 gcacgcgctt cgccgcgctg gccgcggccg gcaagctcga ggcgctcgac gccgaccgcg
     1321 ccgcggcgct ttacgagcta accgccgagc gcaccgccgc cgcgggcctg cccgcctatg
     1381 agatcagcaa ccacgcccga cccggcgagg agagccgcca caacctcgcc tactggcgct
     1441 atggcgatta tgccggagtc ggccccggcg cccacggccg ccgcctcgcc cggcgcacgg
     1501 tccgccaccg caagcccgag aatttcctct ccgccctcgc ccgcgacggc cacggcatcg
     1561 ccgaagacga gccgctggcc gccacccagg ccgccgacga agcgctggtg atggggttaa
     1621 ggctggccga gggcatcgac gccggctcca ttgccgagcg cttcggcctc gattccatcg
     1681 tcgactggac ggcggtcgac cggctcgccc gaagcggcca tgtggagcgc gacggcggcc
     1741 gaatcgcggc gaccccggcg ggccggctgg tgctggatcg cgtgctcgcc gagattgcgg
     1801 tcggctgagc cgcctactgg cgggccgcca tcgcccgata ccgctcgacc agattgtcga
     1861 ccagatcgtt gacctggagc tgggcgtcgg ccgccgcctg cttgcgcggc accttgttct
     1921 tcaggtcgaa cgcgatcagc gccccctcga agctttcctc gatcgacgtg caagcctggc
     1981 gaagatgggc gtcgagcgcc tcggcgggca ttttctgcgt cttggcgctt tcgaacgcct
     2041 gggtgaggca ggcctcatac ttgtcgcgct ggccgttgat cgcggcgctc tggagaagcg
     2101 ctgcgaggat gattgatgtg aacgccgtga acatttcccg gacctcttaa gacccgataa
     2161 tcacaggaga atgcgccttt ttcgagcgtt tgcaaagggg aatttccgac gcttccccaa
     2221 gggcttgctt gcagccccgg cggggatgat gcgatgcttg gggccagcct atggacgagc
     2281 ccctgccccc cggcctctac gtcgtcgcga ctccgatcgg caatctcgcc gacctgtcgc
     2341 cgcgcgccgc cgagacgctg cgccgcgcgg accgcatcct ggccgaggac aagagggtca
     2401 gcgccaagct gctcgcgcac gccggcgcca gggcgccgat ggcgacctac cacgaccaca
     2461 cgagcgaagc ggagcgcgcc gcgatcgtcg cccggctcgg aaccgaggcc gtcgcgctga
     2521 tcagcgacgc cgggactccg ctgatctccg accccggcta caaattggtc cgggcggcgc
     2581 gcgccgccgg ccatccggtg cacacggtgc cggggccgtg cgcggcggtc gccgcattga
     2641 ccctagccgg cctgccgacc gaccgcttcc tgttcaccgg attcctgccg cccaaggaga
     2701 aagcgcgcaa tgacgcgatc ggcgagatcg ccggcatccg ggcgaccctg atcctctacg
     2761 aaaccggacc ccggctggcc ggcacgctcg ccgctctcgc gcagggcctc ggcgcgcgcg
     2821 aggcggcggt cgcgcgcgaa atcagcaagc tgcacgagga gtgcgtgacc ggaacgctgg
     2881 ccgagcttgc ggagcgctat gccggtgccg cgcgcagggg cgagatcgtc atcgtcgtcg
     2941 gcccgccggc cgcgggcgaa gggccaagcg agtccgacct cgatgccgcg ctcggcgagg
     3001 cactggcccg actgtcgccg tcgcgcgccg cggccgaggt cgcggcacgg ctcggggtgc
     3061 cgaggaagcg cgcctatgcg cgggcgcttc agctggcggc gcaatgaacc gatacgcggc
     3121 ggaaaagcgc ggccgcggcg ccgagacgct ggccgcgctg tggctccggc tgcacggctg
     3181 gcggatcgtc gcgcgccgcg cccgagtgcc cggcggcgaa gtcgacatcg tcgcgcgccg
     3241 cggccggacg ctcgcgttcg tcgaggtcaa ggcgcgcgcg accgaggacg cggccgtatt
     3301 ctcgctcgat gcctaccggc tgcgccgggt ggccgtcgcc gccgagcggc tcgcgccgcg
     3361 ctacatgcgc gacggcgacg agctccggat cgacgcgatc ttcatcgtcc cgcgccgctg
     3421 gccgcgccat ctcgccgaca tctggcaggg ataggctcgg ggtgacaaag gcgggcggct
     3481 cgcctaggcg cgcggccatg ccgcttcgcg tcgcagtgca gatggacccg ctcgaggcga
     3541 tcaacatcgc cgccgattcg accttcgcga tcatgctcaa ggcgcaagag atcgggcaca
     3601 gcctccatca ttacacgccc ggggacctca gctacgctca agggcgcctg tggacgatgg
     3661 cgcgcccggt cacggtgcag ccggtggccg gcgatcatta ccgcttcggc gagccggaaa
     3721 tcctcgatct cggccgcgac gtggatgtcg tgctgatgcg ccaggatccg cccttcgacc
     3781 tgggctatat caccgccacc catctgctcg agcggatcca gggcgagacg ctggtggtca
     3841 atgacccggc cagcgtccgc aacgcgcccg aaaagctgtg ggtgctcgac ttcgcgcagt
     3901 tcatgccgcc gacgctggtt acccggtcgc tggggctgac ccgcaaattc ctcgccgagc
     3961 atggcgagat cgtcgtcaag ccgctgcacg gcttcgccgg cgggtcgatc ttccgcatcg
     4021 gccgcgacgc tcagaacctg gcctcgctga tcgagctgtt caacggcgcc tatcgcgaac
     4081 cgcatgtcgt gcaggcgttt ctccccgaga tcgccgaggg cgacaagcgc atcgtcctga
     4141 tcgacggcga ggtcgcgggc gccatcaacc ggctccccgg cgaaggcgag atccgctcca
     4201 acctcgccgt cggcggcaag ccggccaaga cgcagctcac ggcgcgcgag gaggagattt
     4261 gcgccgcgct cgcccccgag gtccggaagc gcggcctgct gttcgtcggc ctcgacgtga
     4321 tcggcggcaa atggctgacc gagatcaacg tcacttcccc aaccggaatc gtcgccatcg
     4381 acgcgctgaa cgggagcgat acgcccgcca tgttctgggc ggccgtcgag cgccgcctcg
     4441 gcgagcgcgg ccgcggccga accggatgag cgccttttca tgagcgactg ggtgatccgc
     4501 ctgatcgagc aatcgggcta cctgggcgtc gccttcctga tgttcctcga gaccatcttc
     4561 ccgccgatcc cgtccgaggt gatcatgccg atcgccggca tggccgcggc caagggcaag
     4621 ctcggattca tccccgtggt cgcgtcgggc accgccgggg cgatgctcgg caacaccgtc
     4681 tggtacctcg ccgcccgagc cctcggggtt caccggctga agccgctgat cacccgctac
     4741 gggcgctggc tgacgatcag ctggcccgag gtcgcccggg ccgagcgctg gttcgcccag
     4801 cacggcacgt ttttcgtgtt cttcggccgg ctgctgccga ccgtccgctc cttggtgtcg
     4861 gttccggccg ggctgcttcg gatgcgcttc aagagcttct ttgcagcctc gctgctcggg
     4921 accgccggct ggacgacgat cctcgccggc gccggctaca agctgcagga caatgtcacc
     4981 gaggtcgagc ggatggtcgg cccggtgtcc aacgcagtgc tggttgtgct cgccgccggc
     5041 tatgtgtggc ggctatggac ccaccgccat caggacgacc gcagagaaga atccgatttg
     5101 gattaactta tctgaagaat ttgcgcggtc ggcagcttcc aaaagcacca tatcggttct
     5161 ttagtggact gtttaccggc cttgcttcaa gccggcgggg ccgcggcgcg accgtctccc
     5221 ccactggccg gtgacgccgc ggcaaacctt ccggaaaaag cgaggctcag gcgcgcggat
     5281 gcgcgtggcg gtagacgtcg agcagccgcg ccgcgtcgac cgcggtgtag atctgggtcg
     5341 acgacaggct cgcgtggccg agcagctcct ggagcgcccg aaggtcggcg ccgcgggcca
     5401 gcagatgggt cgcgaagctg tggcgcagcg cgtgcggggt gaggctgtcg ggcaggccca
     5461 gccgctggcg cgccgagcgc accgagcgcc gcaccagatc gccgttcaac ggcccgcccc
     5521 gcgcgccgac gaacaaagtc gcgtcgccgg tcagttggaa cgggcactgg cggacataat
     5581 cctcgatcgc ctgggcgacc gcgggcacga tcggcaccac ccgcgacttg gagcgcttgc
     5641 cgatcacccg gatcgccgcc ccgaccggaa gcacgcgagc ggtcagcgac agcgcttcgg
     5701 cgacccgcag cccggcgccg tagagcagca gcaggattgc caggtcgcgc gccccgatcc
     5761 attcgactga ggccgccccc gccgcgtcgt gcgcgagccc catcgcatcc tcgggcgaag
     5821 ccgggcgcgg cagggtccgc ggccgcttgg gagcccgagt gcgcggcagc tgcgcggtct
     5881 cgccctgctg ctcggccgca tagttcagga acgcacgcac cgccgacagc tcgcgcgccg
     5941 ccgacgaggc gccgagcccg cttgcccgcc ggtcggcgag aaaggcgcgc aggtcggacg
     6001 cattgagcgc cagcagggcg aagcggccga tctgctcgcc gcggtggcgg ccgaggaaat
     6061 cgacgaagcg gtgggcggtc gcgacatagg cgcgcaccgt gtgcgacgag cgccttcggt
     6121 cgtgctggag atagttgccc cagcgctcgg ccagagcccg cgccggttgc gcttcggtca
     6181 gttcttctgg agcgggtcgg tcagccaccg acgcagcata gcgccaaggc tgcggcctag
     6241 gaacatcagc aattccgagc cgtggcgggc atcgaggttc tgggtcgtgc gctggccgag
     6301 cgcgatcagg ccgcatggaa gcggcggctc gcaatcgatc cggatcagcg cctcggcgcg
     6361 gatcaggtcg caggccggcc cgaacagcgg atggccccgc gccacggtgc gcatctcgac
     6421 cccgtcgacc tgctcgatgg cgcgccgcag gatctgcggg tcgaccgctt ggacgccatt
     6481 gccgtcggcg cggaatccct ggtcgccgac gatcagcgac agcgccacgg cgtcgatgcc
     6541 gaggatcagc ggccattcct gggtgacgac gtgcagcagc gcctcgatgc tgtcggcctc
     6601 gagcgcggcg atcaccgccg cgtggatcgc cgacaccgcg cccgaatggc cgcgcgcgaa
     6661 ggcgatcaga tcctggttgg cttcctcgat cacgccgagc cgttcgcgca ggtgcgcgac
     6721 cgcccgttcc tcgaactgga tcacccgtcc catgagccca agtgtaaccc ggggacggtt
     6781 aatttcctac caccccgccg gggtgcccgg gcgagcgcga gttaccgccc ggccagctcg
     6841 gcgacgaagc tccttgcttc ctggacttcg gcgcggcctc cgtcgctcgc ctgggtcagc
     6901 ttgacgagct cgccataatg acgacgggcg gcagcggcat tgccggcgag cttcgccgat
     6961 cgcgccgcgc catagagtcc ggcgagccgc cgcggcgcgc gcttcagcga cgcctcgaac
     7021 tcggcctgtg cctcggccgg ccgtccaagc tccagaagca gctcgccgag ctgctcgcgc
     7081 gccggcagga tggcgcccgg agtcaccgga tgtttttcgg tcgcatcgtc gaggtccgcg
     7141 gccgaccgca tcaaccgaac cgaatcctcc tttcggcctt cggcgaaagc gagccacgct
     7201 tcggcgatct gccgctcgat cgacacctgg gtcttccagt cataggcccc cggagctgta
     7261 accagcgact gctcgagctc ggcgagcctg gcggcctgcg cccgggcgag cgcggggtcg
     7321 ccgctgcgcg ccgctccgac ggcgttggcg aagcggatat tggcgatcgc ccatttgttg
     7381 tcggtcaggg ccttgaggcc gccgaggtcc gccggcaggt cgaaccccgc ggcttccttc
     7441 cactgccggc gctcgagcag cacccgcgcg gggaccgcgc tgaccgcgta ggcggtcgtc
     7501 ggggtcgaag tcgcctgctt gatcgcctgc agctcgtcga gcacccgctg ggcgtcgacg
     7561 tcgcggccgg tctgcaaata ggcgtaagcg agatagtcca tggcgtgcag ctgctggtcc
     7621 catgtgccgg ccattccgct tgcccgcgca tgggccatcg ccgcggcctg cgcggcgcgg
     7681 ttggagcgga tcgcctcgtc ccacagcccg agccgggtga agatgtgcga gggcatgtgc
     7741 tgcgcgtgcg ccgaggccgg cgcgatgctg gcgtagcggc gcgccgccgg aagcgcgagc
     7801 tcggccagcg ggggataatc gaagctgtgg atcaggtaat gggcgacgcc cggatgatcg
     7861 ggctcctcct tgagcacctg gttgaggatc gcggccgcgc ttttctcacg ctcgaaattg
     7921 ggatccttgt cgagcgttcc cgcggccacc tgagacaagg catagaagac cgccgcctcg
     7981 tcatcgtccg gatagcgctc gcgaagctgc gccatggccg aattataggc cagcgcccgg
     8041 gccttgtggt cgaggcgctc gctatggtcg tagaatgcgc cgatcgcggc gacataatcc
     8101 tgctcgcgct tgctcttcgc tccggccgcc cgcgccttgg ccagcgccgc cttgcccttg
     8161 tcgagctcgg cggccgtcgg cggcgcccag agcggatggt aattggccat ggcgacgccc
     8221 caatgagcga tggcgcagcc ggggtcggtg tcggcggcct tcagaaactg cttctccgca
     8281 cgatcatatt cgaacgagtg gagccagcgc agaccgagct cgaactcggc gtccgccgga
     8341 gcgctgcagc tggtctcgaa ggcaagcttg ccgagcttgg gggaatggtc gtgttgctcg
     8401 ccgggcgaca gagcgagcgc ggcggcggaa gtcagaagca gactgatcat catcggtaac
     8461 ctccaatcga tctaaccgat tttctgcccg ctcgcctgcc agtccttgag gaagctcgcg
     8521 atcccggcgt cggtcagcgg gtgcttgaac aattggtgga tgatcttggg cggcgcggtg
     8581 atcacgtcgg cgccgatctt ggccgcctcg atgacgtgga ccgggtggcg gacgctggcc
     8641 accaggatct gggtcccgaa gtcgtaattg tcgtagatca tgcggatgtc ggcgaccaat
     8701 tgcatgccgt cgaagccgac gtcgtcgtgg cggccgacga acggcgagat gaagctcgcc
     8761 ccggccttgg ccgcgagcag cgcctgggcg gccgaaaagc acaaggtcac gttgaccatc
     8821 gtcccctcgc cggtcagcgc cttgcacgcc ttgaggccgt cgggggtcag cggcaccttg
     8881 atggcgatgt tcttggcgac cttgcgaagc acctcggcct cgcggatcat gctgtcgtag
     8941 tcgagcgcga cgacctcggc cgagaccggg cccgggcaga tcttggaaat ctcgcgcaga
     9001 acctcgatga aatcgcgccc cgacttgtgg accaggctcg ggttggtcgt caccccgtcg
     9061 agcagcccgg tggcggcaag ctcgcggatc tcgtcgatct cggcggtgtc ggcgaagaat
     9121 ttcatgcgtc ggctggccct tggctgcgtg gctttgccgg gcaatagccg ctggcgggca
     9181 atccgtcatc cgattcggcg agaggaaacg cgatgagcga gcaggtgcgg atcgaaagcg
     9241 gcggcggggt gctcgccatc acgctcgccc ggcccgagcg gcgcaacgcg atcaccgtcg
     9301 ccatgtacga ggcgctcgcc gacgccatcg gcaaggcgca agacgacccg tcgctccggc
     9361 tgatcacgct gcgcggcgaa ggcgaggact tcaccgccgg caacgaccta gccgacttcc
     9421 tcgccgccat gccgcgcgag ggcggggagg aaattgccgt ctggcggctg ctccgagcgc
     9481 tcgccgccaa cgatttgccg ctggtcgccg cggtgcacgg caatgcgatc ggcatcggca
     9541 cgaccctgct gctccactgc gacgtcgtcc tggccgagga cggcacccgc ttctcgcttc
     9601 cgttcgtcga cctgggactg gtcccggagg cggcgagctc gttgcttctg ccgcgcctcg
     9661 ccggccgccg gcgcgccgcc cgctacctgc tgctcgccga gccgttcggg gccgaggagg
     9721 cgagcgagtg ggggctggtg accaggatcg tgccgaaggg cgagctcgac ggggcgctgg
     9781 ccgacaccgt cgccgccttg ctcgccaagc ccgccgaagc gctgcgcctc acccaaggcc
     9841 tgctccgaca cggcggcaag gaggagctgc tcgagcgctt cgagctcgag aaccgccact
     9901 tttcggagcg gctccagtcg gccgaggtca aggaggcgat caccgccttt ttcgagaagc
     9961 ggaaacccga tttcaccaag ctgggctgaa ccggcctcgg ccgggccggg tcagatggcg
    10021 atgtagacct tcttgctgcg ggtgagggtc acggtcgggc cgggcacgag caggaagtcc
    10081 atcggcgtcg tgtaggtgac cgtcagcgtc ttgaacccgg tgccgtcgac cggcgcggcg
    10141 acggtgaacg acccgtagcc gttgccgaac agcttggacg tcatccgggt ctggatctgc
    10201 gagttggtcg gggtcgggta gatggtcgcg tagcgcgcgc cctcgccgag cgcgtgctgc
    10261 atcccggcgt tcgcctggaa caatagcccg agccggaaga tgccgtagat gaacagcacc
    10321 aggatcggca ccgcgagggc gaattcgatc gccgcggcgc cgcgctcgtt acgccgaagg
    10381 aaagtgaccc tgctcattgc gtccgaagcc ccgcttcgcc ggtgatcgtg aaggtgccgt
    10441 ccgcgttc
//
LOCUS       DASTZO010000105         2052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_216596,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000105 DASTZO010000000
VERSION     DASTZO010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_216596"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VFU20_02950"
     CDS             <1..528
                     /locus_tag="VFU20_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HEU5285453.1"
                     /translation="GTVLTTEQCLLNPNRNPGLLREDIERRLARDLGFERVVWLGSGL
                     LNDHTDGHVDNIARFVAPGRVAIPSPADGDPNAAVYKSAAERLREAKLDLVTLPSPGR
                     IEGADGDVIPASYMNFYIGNAAVVVPQYGVANDSATVTVVQALFPDRKAVGLRADHIL
                     TGGGSFHCISQQIPR"
     gene            539..1390
                     /gene="aguB"
                     /locus_tag="VFU20_02955"
     CDS             539..1390
                     /gene="aguB"
                     /locus_tag="VFU20_02955"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEU5285454.1"
                     /translation="MTKLTVAALQLAFTEDTVANIRAVADLVRDAHSKGAEVVLPPEL
                     FEGPYFCRTEDESRFADARPAGEHPSVLAMQTLAAELKIWIPTSFFEADGPHYYNSLA
                     MVGPDGKIAGVYRKSHIPDGPGYEEKFYFRPGNTGFKVWNGPHSSSLGVGVCWDQWYP
                     ETARAMVLMGADILFYPTAIGSEPHDPELDTSRLWRRAMIGHAVSNVVPIVAANRIGS
                     EDGQLFYGHSFICDERGDILAELGAEESGALVATLDLGAAKKHRAAFGFFRDRRPELY
                     GRLVEDV"
     gene            complement(1363..2013)
                     /locus_tag="VFU20_02960"
     CDS             complement(1363..2013)
                     /locus_tag="VFU20_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU5285455.1"
                     /translation="MTEELPQRDCQLCPRLAAFRAVQREAHPDWFNAPVPSFGDASPE
                     LLIVGLAPGLRGANRTGRPFTGDFAGDLLYATLLKFGLAEGEYRADPADGLRLTGAVI
                     LNAVKCLPPANKPLPQEIATCRHYLDAALVALPRVRVVVALGQIAHVAAARALGLPPS
                     STRFGHGAEHRAPDGRVLLSSYHCSRYNQNTRRLDAAMFESVFERALAYTSSTNRP"
BASE COUNT          337 a          717 c          697 g          301 t
ORIGIN      
        1 gggaccgtgc tcaccaccga gcaatgcctg ctcaacccca accgcaaccc cgggctgctc
       61 cgcgaggaca tcgagcggcg gctggcgcgc gacctcggct tcgagcgcgt cgtctggctc
      121 ggctcggggc tgctcaacga ccataccgac ggccacgtcg acaatatcgc ccgcttcgtc
      181 gctccggggc gggtggcgat cccgagcccg gccgacggcg accccaacgc agcggtctac
      241 aagagcgcgg cggagcggct gcgcgaggcg aagctcgacc tcgtcacttt gccctcgccc
      301 ggccggatcg aaggcgcgga cggcgacgtc atcccggcga gctacatgaa tttctacatc
      361 ggcaacgccg cggtggtcgt gccccagtac ggggtcgcca acgactcggc cacggtgacc
      421 gtggtgcagg ctttgtttcc tgaccggaaa gccgtgggcc ttcgcgccga ccatatcctg
      481 accggcggcg gcagcttcca ttgcatcagc cagcagattc cgcgctaaag gcgccctgat
      541 gaccaagctc accgtcgccg cgctccagct cgccttcacc gaggacacgg tcgccaacat
      601 ccgcgcggtc gcggacctgg tgcgcgacgc gcattccaag ggcgccgagg tcgtgctgcc
      661 gcccgagctg ttcgagggcc cttatttctg ccgaaccgag gacgagagcc gcttcgccga
      721 cgcgcggccg gccggcgagc acccgtcggt gctggccatg cagacgctcg cggccgagct
      781 gaagatctgg atcccgacca gcttcttcga ggccgacggc ccgcattatt acaacagcct
      841 ggcgatggtc ggccccgacg gcaagatcgc cggcgtctac cgcaagagcc acatcccaga
      901 cggcccgggc tatgaggaga aattctattt ccggcccggc aacaccggct tcaaggtgtg
      961 gaacggcccc cattcgtcga gcctcggggt cggcgtctgc tgggaccaat ggtacccgga
     1021 aaccgcccgg gcgatggtac tgatgggcgc cgacatattg ttctacccaa ccgcgatcgg
     1081 ctccgagccg cacgaccccg agctcgacac gagccggctt tggcgccggg cgatgatcgg
     1141 ccacgcggtc agcaacgtcg tgccgatcgt cgccgccaac cgcatcggca gtgaggacgg
     1201 ccagcttttc tacggccaca gcttcatctg cgacgagcgc ggcgacatcc tcgccgaact
     1261 cggagcggag gaaagcggag cgctggtcgc aacgctcgat ctcggcgccg ccaagaagca
     1321 ccgcgccgcc ttcggcttct tccgcgaccg caggccggag ctctacggtc gattggtgga
     1381 ggacgtgtag gcgagcgccc gctcgaacac gctctcgaac atcgccgcgt cgagccgccg
     1441 cgtattctgg ttgtagcgcg agcagtgata gctcgacagc agcacgcggc cgtcgggcgc
     1501 gcggtgctcg gcgccgtggc caaagcgggt cgaggacggc gggagcccca gcgctcgcgc
     1561 cgcggcgacg tgggcgatct ggccgagcgc gacgaccacc cgcaccctgg gcagggcgac
     1621 cagcgcggcg tcgagataat ggcggcaggt ggcgatctcc tgcggcagcg gcttgttcgc
     1681 cggcggcagg cacttgaccg cgttcaggat caccgcgccc gtgagcctga gcccgtcggc
     1741 ggggtcggcg cgatattcgc cctccgcgag gccgaacttg agcaatgtcg cgtagagcag
     1801 gtcgccggcg aaatcgccgg tgaacgggcg gccggtgcgg ttggcgccac gcagccccgg
     1861 cgccaggccg acgatcagca gctccggcga ggcatcgccg aacgacggca ccggggcgtt
     1921 gaaccagtcc ggatgcgcct ctcgctggac ggcgcggaac gcggcgagcc gcgggcacag
     1981 ctggcagtcg cgctgcggca gttcttctgt caagccggac ctttacgggt agtgactcag
     2041 tttgaatcta at
//
LOCUS       DASTZO010000106         7802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_922470,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000106 DASTZO010000000
VERSION     DASTZO010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7802
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922470"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1095)
                     /locus_tag="VFU20_02965"
     CDS             complement(<1..1095)
                     /locus_tag="VFU20_02965"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEU5285456.1"
                     /translation="MARVFLSYDHEDVARAAPIASALEKAGHSVWWDRQIHGGAEYQT
                     EIESAVQRADAVVVLWSGRSIQSAWVRDEAAEGRDQSKLVPVLLEPVRPPMGFRQYQT
                     IDLSSRRRVSGPTQLDQLLRAVTKMSGRAPSTQQSLSPAVAAQSFDRRAVLAGGAVAT
                     AAAIGIGGWALLKPKSAGAPDSIAVLPFKNLSGDPAQAYFAAGVAGEIRNILTRIAGL
                     KVAGSTSSEAVREEDAQTAAKKLGVINILTGNVRQTPSTIRISAELIDGRTGLAKWSQ
                     NYDRAPGDVIRIQTEIAENVTRALAVALGASARKALAAETSNVAAQTLAFQARDLSWQ
                     LTVPALQRCLQLLDQAISLDPNYARAYALKS"
     gene            complement(1321..2418)
                     /gene="lptG"
                     /locus_tag="VFU20_02970"
     CDS             complement(1321..2418)
                     /gene="lptG"
                     /locus_tag="VFU20_02970"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HEU5285457.1"
                     /translation="MINLHFFPSRRIAFYTVRLFLTRSLAVLVALVLILMTLDLLGES
                     GKILAVPGNGDAELWRYVALRVPLLTARFLPFSVLLGTLIAFAGLNQHSEVIAMKAAG
                     ISAHQILAPLIVASLGIAALAFAFNETVVVKAARTVTAWSDNDYRPIPPDSDLLSNVW
                     VLVGDDLVRARQVVGQGQRLRATGLSIYDREGGTLARVIDARRARPEGAAWRLEDVRI
                     YDATMNLVRRHEQMTALRGVEPQRLTLAKVDPDQLDFFTLRERIGQMERAGRPTEEAR
                     AGLWHKISGPLSTLLMPLLAAVAAFGLARSGQVLLRATVGMALGFAYFVADNFSLAMG
                     NVGAYPPLLAAWAPFVLFALIGETVLIRSEE"
     gene            complement(2415..3611)
                     /gene="lptF"
                     /locus_tag="VFU20_02975"
     CDS             complement(2415..3611)
                     /gene="lptF"
                     /locus_tag="VFU20_02975"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HEU5285458.1"
                     /translation="MGRLSLIDRYLARSIAVPLLGTLILAAMLLVLDKMLRLFDFVVT
                     AGGPVSVVWRMLANLLPEYFSLGIPIGLMLGILLAFRKLALSSELDALRGIGIGFGRL
                     LRVPYAYAAALMLLNLAIVGYIEPYARYRYEGLRFDLRSGALGASIKVGEFNRLGRRL
                     TLRIDGSEDSGTRLRGIFVQVDDRSGTSVAATAATGRFLSTDDPDTILFRLRSGRLVQ
                     QSPKFAGPRTLSFDSYDLPINLPQVDRFRNRGHEHDELTLNELFDEGYGGGATGRDRL
                     AAQANLHFRLVEVLMMMMLPLLAVALAVPPKRSSSSLGIFIGIVIVVAYHKVNQYAEQ
                     AAAQGRIEPIVALWVPLVLLSALIVWMYHVLAHQPGGQPIGALERAFARVGRAVRSLL
                     PKPQRA"
     gene            3737..4234
                     /locus_tag="VFU20_02980"
     CDS             3737..4234
                     /locus_tag="VFU20_02980"
                     /inference="COORDINATES: protein motif:HMM:NF021435.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2141 domain-containing protein"
                     /protein_id="HEU5285459.1"
                     /translation="MSKTSLALAAALLAGMAAAAPAPAKVVGSDAEACLTGKPSMLVR
                     VSGLKKPSGTVKVAVYAAHGYLAKRGKLRKVVVPVRSTAPMDVCIAVPKAGQYAVAVH
                     HDLNGNGDKDLSDGGGYSGNPRLSITNLKPPFGKTAVRVGAAPRHVTVLLQYRDGLSI
                     RPVNG"
     gene            4234..5208
                     /locus_tag="VFU20_02985"
     CDS             4234..5208
                     /locus_tag="VFU20_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylglycerol kinase family protein"
                     /protein_id="HEU5285460.1"
                     /translation="MARPRIALLSNPKSTGNLAQLPRIRAFCADHPDIFHYEVEKAHQ
                     VGAALKTIARVRPKMLVINGGDGTVQAALTELYNGGHFAKEPPPVAVLPSGKTNLIAL
                     DLGAPGDPIAALEKLLEIAQADLSPHLVARELIALRCGAGEQRPVIGMFLGGAGLADT
                     MLYCREKIYPLGLPNGVSHALTALALLARLFLRLKASFLPPDPSPLAVKWRNSQSLTG
                     RFSLLAVTTLDRLLLSGEIRGHNRGVLRLVAIDERPASVLRAFFATLLGKLGHSKLRG
                     VHFEESDEISIEGESSRVILDGETFRAEIGRPIFLKLAQPLSFVRIAA"
     gene            5219..6112
                     /locus_tag="VFU20_02990"
     CDS             5219..6112
                     /locus_tag="VFU20_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285461.1"
                     /translation="MVELRRLVAAELASAVDPRVAAMAGAIAALHGKASRAVLFYGSC
                     LREKQLDGLMLDFYLIVSDYRAAYDQRWLAVANRLVPPNVFCFEHHGLTAKYAVLSED
                     DFARECDVSARTPSVLARFAQPSRLVWAKDDAARTRAVDAVSRAAPALLHLTQPTMSD
                     GEAADPLAIWKKAFAYTYGAELRAERKSRPDAIVEADPERYRQFARAAGFPFPLETTE
                     SERRMTDEAWRRFQRRTRRIQMLRLAKASATFTGGVDYIAWKINRHAGTQIRIKPWQR
                     RWPLLAAISLLPRLLRSGAVR"
     gene            complement(6069..>7802)
                     /locus_tag="VFU20_02995"
     CDS             complement(6069..>7802)
                     /locus_tag="VFU20_02995"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5285462.1"
                     /translation="TSGSIYYSQWRLAGHQINQLERLVRTSPEQRRRVNELLQLYAQR
                     GAELAPAARAAAVKGQGAISYYYAAGEARTGPAVISKLDEIADSERQSLRERIEQSEI
                     FSAQADRLTDYLSWLGIIVGIGAIFLGLVAVQAVRQNAAARRQAESESARAEALEQAV
                     DERTRELQAANEALRAEAVERQLAEAQLRQVQKMEAVGQLTGGIAHDFNNMLAVVVGG
                     IDLARRRLNGPKREVMAHLTNAMEGATRAAALTRRLLSFARSEPLLPERVDSSELVAG
                     MSDLLDRTLGERIQVEVDLAPDAWPTYVDPHQLENAIVNLAVNARDAMDGTGVMRITT
                     SNVTVKANEVGDIRPGEYVRLSVSDTGCGMTPEVRERAFEPFFTTKPVGKGTGLGLSQ
                     IFGFAHQSGGEVGIESEVGKGTTVSIYLQRTESAAAAVRLHPAVQRTEGEAHVAGARI
                     LLVEDDPRVRAATVDALEDLDYQPIACSSGAEAIRLFESQEFDLVISDVIMPEMTGPE
                     LIRYLKASPRDFAVLFVTGYVGEGETDHLVGYELLRKPFTVGALANAVASALARRSSE
                     PRQTSGVAAKG"
BASE COUNT         1251 a         2635 c         2697 g         1219 t
ORIGIN      
        1 ggatttgagc gcataggcgc gcgcatagtt cggatcgagc gaaatggcct ggtcgagcag
       61 ttgcaggcag cgctggagcg ccggaacagt cagttgccag ctcaagtccc gtgcctggaa
      121 ggcaagggtc tgcgcagcaa cgttgctggt ctcggccgcc agcgcctttc tggcgcttgc
      181 gccgagggcg acagcaagtg cgcgcgtgac attctcggcg atctcagttt gaatcctgat
      241 cacatcaccg ggagcgcggt cataattttg cgaccatttg gcgaggcccg tgcggccgtc
      301 gatcaactcg gctgagatgc gtatcgttga cggcgtttgg cggacattcc ccgtcaggat
      361 gttgatgact ccaagcttct ttgccgccgt ttgagcgtcc tcctcccgga cggcctcgga
      421 cgacgtgctg cccgctacct tcagaccggc aatccgcgtg agaatatttc tgatttctcc
      481 ggcgacgccg gcggcaaaat aagcctgcgc gggatccccg ctgaggttct tgaaggggag
      541 cacggcgatg ctgtccggcg cgccggccga tttgggcttc agaagcgccc atccaccgat
      601 tccaatggcg gccgcagtag cgacagctcc tccggccaga accgcgcgtc ggtcgaaact
      661 ctgtgccgca acggcgggcg aaagcgactg ttgagtggaa ggagcgcggc cgctcatctt
      721 ggtgacggcg cgcagcagct gatccagctg ggtcggtccg gaaacccgcc tccggctcga
      781 cagatcgatc gtttgatatt ggcggaagcc catcggcggc ctcaccggct ccaggagcac
      841 cggaaccagc ttgctctgat ctctgccctc ggcagcttca tcgcgaaccc aagctgattg
      901 gatcgatctt ccggaccaca gcacgacgac cgcatctgct cgctggactg cgctttcgat
      961 ttcggtctgg tactcggcgc cgccgtggat ctggcggtcc caccaaaccg aatggccggc
     1021 tttctcaagc gctgaagcga taggcgccgc gcgcgcgacg tcctcgtgat catagctcaa
     1081 gaataccctg gccatgcgca gccctctggg cttgatcgtg cccctaaata gtcgcttggg
     1141 caaggatcgc agtcgaaccg ctgacctcac ggttagctgt gaatgcggac attagccacc
     1201 atgtgtccaa aaggaataat cgctcgcggt tttaggtcgt gacggctcga ccattcccgc
     1261 ggaatgacct gcgcctgagc gtgcgtgctc ggctgatccc ccggatcagc ctacacgcgc
     1321 tcattcctcg ctgcggatca gcaccgtctc gccgatcagc gcgaacagga cgaacggcgc
     1381 ccaggcggcg agcagcggcg ggtaggcgcc gacattgccc atcgccagcg agaaattgtc
     1441 ggcgacgaaa taagcgaagc ccaatgccat cccgaccgtc gcccggagca gcacctggcc
     1501 cgagcgggcg aggccgaacg cggcgaccgc ggccaagagc ggcatcagca gggtcgacag
     1561 cggcccggag atcttgtgcc acaggccggc gcgggcctcc tcggtcgggc ggccggcgcg
     1621 ctccatctgg ccgatccgct cccgaagcgt gaagaaatcg agctggtcgg ggtcgacctt
     1681 ggccagggtc agccgctgcg gctcgacgcc ccgaagcgcc gtcatctgct cgtgccggcg
     1741 caccaggttc atggtcgcgt cgtagatgcg cacgtcctcg agccgccagg cggcgccctc
     1801 gggccgcgcg cgcctcgcgt cgatcacccg cgcgagcgtg ccgccctcgc ggtcgtagat
     1861 gctgaggccc gtcgcacgca ggcgctgccc ctggccgacc acctggcggg cccggaccag
     1921 atcgtcgccg accagtaccc agacgttgct gagcaggtcg ctgtcgggcg ggatcggacg
     1981 gtagtcgttg tcgctccacg cggtgacggt gcgcgccgcc ttgaccacca ccgtctcgtt
     2041 gaaggcgaag gcgagagcgg cgatgccgag gctggcgacg atcagcgggg cgaggatctg
     2101 gtgcgcggaa atgcccgccg ccttcatcgc gatcacctcg ctgtgctggt tgaggccggc
     2161 gaaggcgatc aaggtcccga gcagcaccga gaagggcagg aagcgcgcgg tcagaagcgg
     2221 cacccgcagg gcgacgtagc gccacagctc ggcgtcgccg ttgccgggca ccgcgaggat
     2281 cttgcccgat tcgccgagca ggtcgagggt catcaggatc agcaccagcg ccaccagcac
     2341 cgccaggctg cgggtcagga acagccggac ggtgtagaaa gcgatccggc gcgacgggaa
     2401 gaaatgcagg ttgatcatgc gcgctgcggc ttcgggagca gcgagcggac ggcccggccg
     2461 accctggcga aggcgcgctc gagcgccccg atcggctggc cgccgggctg gtgggcgagc
     2521 acgtggtaca tccacacaat cagcgccgag agcagcacca gcggcaccca cagagcgacg
     2581 atcggctcga tccgcccctg ggcggccgcc tgctcggcgt actggttgac cttgtgatag
     2641 gcgacgacga tgacgatgcc gatgaagatg ccgagcgagg acgagcttcg cttcggcggc
     2701 acggcgagcg cgaccgcgag cagcggcagc atcatcatca tcagcacctc gaccagtcgg
     2761 aagtggaggt tggcctgggc ggcgagccgg tcgcggccgg tggcgccgcc gccatagccc
     2821 tcgtcgaaca gctcgttgag ggtgagctcg tcatgctcgt ggccgcggtt ccggaagcgg
     2881 tcgacctggg gcaggttgat cggcaggtcg tagctgtcga acgacagggt ccgcgggcct
     2941 gcgaacttgg gcgactgctg gaccagccgg ccgctgcgca gccggaacag gatcgtgtcg
     3001 gggtcgtcgg tcgacaggaa gcggccggtc gccgcggtcg ccgcgaccga ggtccccgag
     3061 cggtcgtcga cctggacgaa gatgccgcgc agccgggtgc cgctgtcctc gctgccgtcg
     3121 atcctgaggg tcagccgtct gccgagccgg ttgaactcgc cgaccttgat cgacgccccg
     3181 agcgcccccg agcgcaggtc gaagcgcagc ccttcgtagc ggtagcgcgc atagggctcg
     3241 atgtagccga cgatcgccag gttgagcagc atcagcgcgg cggcgtaggc ataaggcacg
     3301 cgcagcagcc ggccgaagcc gatgccgatc ccgcgcagcg cgtcgagctc cgacgacaga
     3361 gcgagcttgc ggaaggcgag caggattccg agcatcagcc cgatcgggat cccgagcgag
     3421 aaatactcgg gcagcagatt ggcgagcatc cgccagacga cgctgaccgg gccgccggcg
     3481 gtgacgacga aatcgaacag ccgcagcatc ttgtcgagca ccagaagcat cgcggcaagg
     3541 atcagcgtgc ccagcagcgg gacggcaatg gaacgcgcca gatagcggtc gatcagagac
     3601 aggcggccca agttttggtc gtccaagcac catgatttgg ccgcaaagcg cgccgagatt
     3661 gcgacacggg cggctctacc cttcccggcg aaggggtgcc gtccgtgtgc gtcgaaaacg
     3721 aacggtctgc aatatcatgt cgaaaacgag tctcgccctt gccgccgccc tgcttgccgg
     3781 gatggcggcg gccgcgcccg ctccggccaa ggtcgtcggc tcggacgccg aggcgtgcct
     3841 gaccggcaag ccgtcgatgc tggtcagggt cagcggcctc aagaagccca gcggcacggt
     3901 caaggtcgcg gtctatgcgg cccacggcta cctcgccaag cgcggcaagc tgcgcaaagt
     3961 ggtggtgccg gtgcgctcga ccgcgccgat ggacgtctgc atcgccgtcc cgaaggcggg
     4021 ccaatatgcg gtcgcggtcc accacgacct caacggcaat ggcgacaagg acttgagcga
     4081 cggcggcggc tattcgggca atccgaggct gtcgatcacc aatctgaagc cgccgttcgg
     4141 caagaccgcg gtgcgggtcg gcgcggcccc gcgccacgtc accgtgctcc tccagtaccg
     4201 cgacggcctg tcgatccggc cagtgaacgg gtgatggcgc ggccccggat cgcgctgctt
     4261 tccaatccca agtcgaccgg gaatctcgcc cagcttccgc ggatccgggc gttttgcgcc
     4321 gaccatcccg acattttcca ttatgaagtg gaaaaggccc accaggtcgg cgccgcgctc
     4381 aagaccatcg cccgggtgcg gcccaagatg ctggtcatca atggcggcga cggcaccgtc
     4441 caggcggccc tgaccgagct ctacaacggc ggccatttcg ccaaggagcc gccgccggtc
     4501 gccgtgctgc cgagcggcaa gaccaacctc atcgcgctgg atctgggagc gcccggcgac
     4561 ccgatcgcgg cgctcgagaa attgctcgag atcgcccagg ccgacctcag cccccacctc
     4621 gtcgcgcgcg agctgatcgc gctgcgctgc ggggcgggcg agcagcggcc ggtgatcggc
     4681 atgttcctcg gcggcgccgg ccttgccgac acgatgcttt actgccgcga gaagatctat
     4741 ccgctcggcc ttcccaacgg cgtcagccac gcgctgaccg cgctggcgct gctggcccgg
     4801 ctgttcctgc ggttgaaggc gagcttcctg ccgcccgacc cgagcccgct ggcggtcaag
     4861 tggcgcaaca gccagagcct caccggccga ttctcgctgc tcgcggtgac gaccctcgac
     4921 cggctgctgc tgtcgggcga gattcgcggc cacaaccgcg gggtcctgcg gctggtggcg
     4981 atcgacgagc gaccggcctc ggtgcttcgg gcgttcttcg cgacgctgct tggcaagctc
     5041 ggccactcga agctgcgcgg ggtccatttc gaggaaagcg acgaaatcag catcgagggc
     5101 gaaagctccc gggtgatcct cgacggcgag accttccgcg ccgagatcgg ccgcccgatc
     5161 ttcctcaagc tcgcccagcc gctgtcgttc gtccgcattg ccgcctgagg gccgggcggt
     5221 ggtcgagctc aggcggctgg tcgcggcgga gctggcgagc gcggtcgatc cgcgggtcgc
     5281 cgcgatggcc ggcgcgatcg ccgcgctgca cggcaaggcg tcgcgggcgg tgctgttcta
     5341 cggctcgtgc ctgcgcgaga agcaactcga cgggctgatg ctcgatttct acctgatcgt
     5401 ttccgattat cgggcggcgt acgaccagcg gtggctggcg gtcgcgaacc ggctcgtccc
     5461 gcccaatgtg ttctgtttcg agcatcacgg actgacggcg aaatacgcag tcctgagcga
     5521 ggatgatttc gctcgcgaat gcgacgtgtc ggcgcgcacg ccctcggtcc tggcccggtt
     5581 cgcccagccg tcgcgcctcg tgtgggcgaa ggacgatgcg gcgcggaccc gggccgtcga
     5641 tgcggtgtcg cgggccgctc cggcgctcct gcacctcacg cagccgacga tgagcgacgg
     5701 ggaggccgcc gacccgctgg cgatctggaa gaaggccttc gcctacacct acggagccga
     5761 gctgcgcgcc gagcgaaagt cgcgcccgga cgcgatcgtc gaggcggatc cggagcggta
     5821 ccggcaattc gcacgggcgg cgggctttcc gttcccgctg gaaacgaccg aatccgagcg
     5881 tcgaatgacc gacgaggcat ggcggcggtt ccagaggcga acccgccgga tacaaatgct
     5941 gcgcctcgcc aaggcgagcg cgaccttcac cggcggggtc gactacatcg cgtggaagat
     6001 caaccgccac gccggcacgc agatccggat caagccgtgg cagcggcgct ggcccttgct
     6061 ggccgcgatc agccttttgc cgcgactcct gaggtctggc gcggttcgct agaccgccgc
     6121 gccagggccg acgccaccgc attggcgagc gctccgacgg tgaacggctt gcgcagcagc
     6181 tcatagccga cgaggtggtc ggtctcgccc tcgccgacat agccggtgac gaacagcacg
     6241 gcgaaatcgc gggggctggc cttcagatag cggatcagct cgggcccggt catctcgggc
     6301 atgatcacgt cgctgatcac caggtcgaac tcctggctct cgaacagccg gatcgcctcg
     6361 gcgccgctcg agcaggcgat cggctggtag tcgaggtcct cgagcgcgtc gaccgtcgcc
     6421 gcccgcaccc gcggatcgtc ctcgaccagg aggattcgcg ccccggcgac gtgggcctcg
     6481 ccctcggtgc gctggacggc cggatgaagg cggaccgccg ccgcggcgga ttcggtgcgt
     6541 tgcagataga tcgagaccgt ggtccccttg ccgacctcgc tttcgatccc gacctcgccg
     6601 cccgactggt gcgcgaagcc gaagatctgg ctgagcccga gcccggtgcc cttgccgacc
     6661 ggcttggtcg tgaagaaggg ctcgaacgcc cgctcgcgga cctcgggggt catgccgcag
     6721 ccggtgtcgc tgacgctgag ccggacatat tcgccggggc ggatgtcgcc gacctcgttc
     6781 gccttgacgg tgacgttgga ggtggtgatg cgcatcaccc cggtgccgtc catcgcgtcg
     6841 cgcgcgttga ccgccagatt gacgatggcg ttctcgagct ggtgggggtc gacataggtc
     6901 ggccaggcgt cgggggcgag gtcgacctcg acctggatgc gctcgcccag cgttcggtcg
     6961 agcaggtcgg acatgccggc gaccagctcg ctcgaatcga cccgctcggg cagcagcggc
     7021 tcggagcgcg cgaacgacag cagccgccgg gtcagcgccg cggcccgggt cgcgccctcc
     7081 atcgcattgg tcagatgcgc catcacctcg cgcttggggc cgttgaggcg gcggcgggcg
     7141 agatcgatcc cgccgaccac caccgccagc atattattga agtcgtgggc gattccgccg
     7201 gtcagctggc cgaccgcctc catcttctgg acctggcgca gctgggcctc ggcgagctgg
     7261 cgctcgaccg cttcggcgcg cagcgcctcg ttggccgcct gcagctcccg cgtgcgctcg
     7321 tcgaccgcct gctcgagcgc ctccgcgcgg gcggattcgg attcggcctg gcggcgggcg
     7381 gcggcattct gccggaccgc ctgcaccgcg accagcccga ggaagattgc gccgatgccg
     7441 acgatgattc ccagccagct gagataatcg gtcagccggt cggcctgggc ggagaagatc
     7501 tcgctctgct cgatgcgctc gcggagcgac tggcgctcgc tgtcggcgat ttcgtcgagc
     7561 ttgctgatga ccgccggccc ggtcctcgcc tcgccggccg cgtaataata ggaaatcgcg
     7621 ccctggccct tgaccgcggc ggcgcgggcg gcgggggcca gctcggcgcc gcgctgggcg
     7681 tacagctgca gcagctcgtt cacccgccgc cgctgctcgg ggctggtgcg caccagccgc
     7741 tcgagctggt tgatctggtg cccggcgagg cgccactggc tgtaatagat gctgccgctg
     7801 gt
//
LOCUS       DASTZO010000107         2751 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1261693,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000107 DASTZO010000000
VERSION     DASTZO010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261693"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="VFU20_03000"
     CDS             <1..252
                     /locus_tag="VFU20_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5285463.1"
                     /translation="EVAVTIEEASVGGLGAHVLTLASDEGLVDAGLKIRTMRLPDRFQ
                     EHDKPEKQYAEAGLDAAGIVDTVLKALRRNSVGIEEVRA"
     gene            249..2153
                     /locus_tag="VFU20_03005"
     CDS             249..2153
                     /locus_tag="VFU20_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HEU5285464.1"
                     /translation="MIASLLIANRGEIACRVIRTARALGVRTVAVYSDADRHALHVRM
                     ADEAVHIGASPARESYLVGERIIAAARDAGADAVHPGYGFLSENADFAQAVIDAGLVW
                     VGPKPGSIRAMGLKDAAKRLMAEAGVPVTPGYLGEDQAPETLQNEAQKIGWPVLIKAV
                     AGGGGKGMRRVDSAADFIDALDSCKREAASSFGDDRVILEKYILRPRHIEVQVFGDTH
                     GNVVHLFERDCSLQRRHQKVIEEAPAPGMDEATRADVCTAAVRAAQAVKYEGAGTVEF
                     IADASQGLEADRIWFMEMNTRLQVEHPVTEMITGVDLVEWQLRVASGEALPKRQDELA
                     LAGHAIEARLYAEDPARGFLPSVGRLEHFELGAGVDQEDGRVDTGVEEGDAISPYYDP
                     MIAKLVAHGSDRGEAIESLAAMLDGTEVWPVRTNAGFLFNALLHEDFGSGEIDTGFIE
                     RNLGELVPGAEPDEAIWRGAAAVAVAMDEEDEPLADLAGFRVNAAPRTSVALGRGGAF
                     RSVALDDEAEMAPVSGFRDEERVVVFTEGQAWEFELASRGSVGGHGVADGVVVAPMPG
                     RVSAVEVAAGDRVDKGQRLLTLEAMKMEHGLVAPFDGTVAEVGAEAGAQVNEGAVLAR
                     IEPSVEPTPA"
     gene            2210..>2751
                     /locus_tag="VFU20_03010"
     CDS             2210..>2751
                     /locus_tag="VFU20_03010"
                     /inference="COORDINATES: protein motif:HMM:NF012309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HEU5285465.1"
                     /translation="MRIARSIALATAATLALGACYTTGEPVGGPTFALVNAAGQPIGS
                     ARMWETPGGVTFRIGASGLPHGIHAIHVHSVGRCDPPGFATSGAHWNPEGRQHGFNNS
                     AGPHRGDMPNVTVSANGVLQEAVSLAGASFGAMLDADGAALVIHARADDYATDPSGNS
                     GERIACGVISLSHQSGTLMGT"
BASE COUNT          428 a          885 c         1076 g          362 t
ORIGIN      
        1 gaggtggcgg tgaccatcga ggaagcctcg gtcggcgggc tcggcgcgca cgtgctgact
       61 ctggccagcg acgagggact ggtcgacgcg gggctgaaga tccgcacgat gcggctgccg
      121 gaccgctttc aggagcatga caagccggag aagcagtacg ccgaggccgg gctcgacgcc
      181 gcgggcatcg tcgacacggt cttgaaggcg ctgcgccgca acagcgtcgg cattgaggaa
      241 gtccgcgcct gatcgcgtcg ctgctcatcg ccaatcgcgg ggagatcgcc tgccgggtga
      301 tccggacggc gcgggcgctc ggggtgcgga cggtggcggt ttattcggat gcggaccggc
      361 acgcgctgca cgtgcggatg gccgacgagg cggtgcacat cggcgcttcg ccggcgcggg
      421 aatcgtacct ggtcggcgag cggatcatcg ccgcggccag ggacgcgggc gcggacgcgg
      481 tccacccggg ttacggattc ctcagcgaga atgcggattt cgcccaggcc gtgatcgacg
      541 cgggcctggt gtgggtcggg ccgaagccgg gcagcatccg cgcgatgggc ctgaaggacg
      601 ccgccaagcg gctgatggcc gaggcgggcg tgccggtgac gccgggctac ctcggcgagg
      661 accaagcgcc cgagaccctg caaaacgagg cgcagaagat cggctggccg gtgctgatca
      721 aggcggtcgc cggcggcggc ggcaagggca tgcggcgggt cgattcggcc gcggatttca
      781 tcgacgcgct cgacagctgc aagcgcgagg cggcgagctc gttcggcgac gaccgcgtga
      841 tcctcgagaa atacatcctc cgcccacggc acatcgaggt gcaggtgttc ggcgatacgc
      901 acggcaatgt cgtccatttg ttcgagcgcg actgctcgct gcagcgccgc caccagaagg
      961 tgatcgagga agcgccggcg ccggggatgg acgaagccac gcgggcagat gtgtgcacgg
     1021 cggcggtgcg ggcggcgcag gcggtcaaat acgagggcgc ggggacggtc gagttcatcg
     1081 ccgacgcatc gcagggcctg gaggcggacc gcatctggtt catggagatg aacacgcggc
     1141 tacaggtcga gcatccggtc accgagatga tcaccggggt cgatctggtc gagtggcagc
     1201 tgcgcgtggc gagcggggag gcgctgccga agcggcagga cgagctggcg cttgccggcc
     1261 acgcgatcga ggctcggctc tacgccgagg acccggccag ggggttcctg ccgagcgtcg
     1321 gccggctcga gcatttcgag ctcggcgcgg gcgtcgacca agaggacggc cgcgtcgaca
     1381 cgggcgtcga agagggcgac gcgatctcgc cctattacga cccgatgatc gccaagctgg
     1441 tcgcgcacgg cagcgaccgc ggcgaggcga tcgagagcct cgcggcgatg ctcgacggca
     1501 ccgaggtgtg gccggtccga accaatgccg gcttcctgtt caacgcgctg ctgcacgagg
     1561 acttcggcag cggcgagatc gacaccggct tcatcgagcg caatctgggc gagctggtgc
     1621 ccggggccga gcccgacgag gcgatctggc gcggcgcggc ggcggtggcg gtggccatgg
     1681 acgaggagga cgagccgctc gccgatctcg ccggcttccg agtcaatgcg gcgccccgga
     1741 ccagcgtcgc gctcggccgc ggcggcgcgt tccgaagcgt cgcgctcgac gacgaagcgg
     1801 agatggcccc ggtgtcgggc ttccgcgacg aggagcgcgt ggtcgtcttc accgaggggc
     1861 aggcgtggga gttcgagctc gcgtcgcgcg gcagcgtcgg cgggcacggg gtcgcggacg
     1921 gggtggtggt cgcgccgatg cccgggcggg tcagcgcggt cgaagttgca gccggagatc
     1981 gcgtcgacaa gggccagcgg ctgctgacgc tcgaggcgat gaagatggag cacgggctgg
     2041 tcgcgccgtt cgacggcacg gtcgcggagg tcggtgccga ggccggagcg caggtcaatg
     2101 agggcgcggt gctggcgcgg atcgagccga gcgtggagcc gacgcccgcc tgacggcatg
     2161 aaccaagtcg cggcggcgcc gttgtcgctg cgacacggga gacacggaca tgcgcattgc
     2221 ccgctcgatc gcactcgcca ccgcggccac cctggcgctg ggcgcctgct acaccaccgg
     2281 cgagccggtc ggcgggccga ccttcgccct ggtcaacgcc gccggccagc cgatcgggtc
     2341 ggcgcggatg tgggagaccc cgggcggggt gaccttccgg atcggagcgt cgggcctgcc
     2401 gcacggaatc cacgcaatcc acgtccactc cgtcggccgc tgcgaccccc ccggctttgc
     2461 gaccagcggc gcgcactgga acccggaggg gcggcagcac ggcttcaaca attccgccgg
     2521 cccgcaccgc ggcgacatgc ccaacgtcac cgtctcggcc aacggcgtgc tgcaggaggc
     2581 ggtcagcctg gccggagcga gcttcggggc gatgctcgac gccgacggcg ccgcgctggt
     2641 gatccacgcc cgggccgacg attatgcgac cgacccgagc ggcaacagcg gcgagcggat
     2701 cgcctgcggg gtgatcagct tatcccatca aagtggtact ttgatgggga c
//
LOCUS       DASTZO010000108         8889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1183468,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000108 DASTZO010000000
VERSION     DASTZO010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 8889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8889
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1183468"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="VFU20_03015"
     CDS             complement(<1..764)
                     /locus_tag="VFU20_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HEU5285466.1"
                     /translation="MTATPAGMTYADYLALDRLLTAQKPLSGLHDEMLFIVIHQTKEL
                     WLKQMLHELKLAIALLDQDHFAASYKAMARIGRIQSVMTLSWDVLSTLTPVDYSAFRD
                     VLGTSSGFQSVQFRELEYRLGIKDPKFLDHYAQGSEARARLRSALDEPSLRDSSHSAL
                     VRAGFDLGDRSEAAVGDAWLEVYRDAERWFDLYQLAEKLVDLDDALAMWRHKHVLTVE
                     RIIGNKPGTGGSAGAAYLRSTLDKRAFPELWALRTEL"
     gene            complement(809..1246)
                     /gene="bamE"
                     /locus_tag="VFU20_03020"
     CDS             complement(809..1246)
                     /gene="bamE"
                     /locus_tag="VFU20_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020424624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HEU5285467.1"
                     /translation="MAVAGAVLLAGCAGIREHRGAVIDKELASAIQVGVDNKDSVAKT
                     LGRPTFTGQFNPNDWYYVSRDTKSVALRGVGVIDQTVLHIRFDAAGNVAAVNRSGKEL
                     IASIDPVNARTPTLGRKRSFFDELFGNIGTISQPGLPGSGRQQ"
     gene            1409..1924
                     /locus_tag="VFU20_03025"
     CDS             1409..1924
                     /locus_tag="VFU20_03025"
                     /inference="COORDINATES: protein motif:HMM:NF015914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HEU5285468.1"
                     /translation="MLSFLFPRLTPSGASRGGALFEALVGEARRPHWYVEGQVPDTLD
                     GRFAVLATLVALATVRLERGGDSARAEEAALAERFVEAMDSEHRELGLGDPAVGKTVR
                     RLVGALARRVDRSRAAVERGDQWDDAVTDSLFRDSPPADALAHCAAALRTIWARLEAA
                     SDEALAQGRIG"
     gene            1921..2451
                     /locus_tag="VFU20_03030"
     CDS             1921..2451
                     /locus_tag="VFU20_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU5285469.1"
                     /translation="MIEDFAHRLTLDRIRDGERIDLVADDEERRAIADRLDLPSLDRL
                     EAHASLARDGDTVRAAGRIAASLEQRCVVTGEPVAAHVDEPFDIAFVPEPRAGDVPEE
                     VELRPEDCDTVFHDGSAIDLGTAIADTLALALDPYPRSASAEAALREAGVLTEAEAGP
                     FAELAKLKKGLGGGDA"
     gene            complement(2482..2985)
                     /gene="ssb"
                     /locus_tag="VFU20_03035"
     CDS             complement(2482..2985)
                     /gene="ssb"
                     /locus_tag="VFU20_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU5285470.1"
                     /translation="MAGVNKVILVGNLGDDPEARSLNSGGEVVNLRVATSEQWKDKDG
                     NRQERTEWHRVVIFNENLGRVAKNYLRKGSKVYLEGQIQTRKWQDQSGADRYSTEIVL
                     QRFRGELVLLDSRGEGGGSRGGGGGGGGDFADEFAGGASSGGGTRTQSRPQPAAFDSD
                     LDDDVPF"
     gene            complement(3034..4968)
                     /locus_tag="VFU20_03040"
     CDS             complement(3034..4968)
                     /locus_tag="VFU20_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HEU5285471.1"
                     /translation="MALLKRFWMLIALAALALPVAPAVAQSQDVQTAWRLLDYIAVDY
                     REAVAGGRIVNRLEYDEMVEFSASVGERLEALPDKPGKPQLVEGAAALRKAIAAKSPP
                     EAIDRHARSLASQLLAAYPVPLAPAQVPDVARGAKLYADNCAACHGANGDAKVPVAAT
                     MDPPPIAFTDRARAGERSPFALYQVIDQGLEGTAMASFRHLPEADKWALAFHSGRFAY
                     PEALAAEGKRLWESDPTLRQRIPDLAALAGLTPAELARAIGESRAAAVIAYLRANPEA
                     VVAPADSNSLALARDRLRQSLAAYEAGDRERAKQLALSAYLDGFEPIEPVLAARDSAL
                     MARVERAMAQLRAAIGAGRPAAEVRQRAAELDAMFADTERVLAPGEAGAASAFVGAFT
                     ILLREGLEALLIVVAMLTFLRKAERPEMVRTVHYGWVAALVAGLGTWWAATHVLTISG
                     ASRELTEGFGSILAALILLFVGIWMHGKAQADEWQRYIKARLGAALSGRSAWFLFLLA
                     FIAVYREVFETILFFAALSAEGNIGALAAGGAAGLVLLAAIALAMFRFSARLPIGKFF
                     AYSSALIAVLAVVLAGKGVAALQEAGLLGVTPVATAPRVAILGLYPTLEGLAAQAATL
                     LVLLLGFGWNRRSSRPLAVV"
     gene            complement(5047..5697)
                     /locus_tag="VFU20_03045"
     CDS             complement(5047..5697)
                     /locus_tag="VFU20_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COQ9 family protein"
                     /protein_id="HEU5285472.1"
                     /translation="MEQPTPLEQLRQRLALAVGEHAVFDGWSSAAIDAAADSLGIDRA
                     QARLAMPKTQAGMVDVYIQGVDRALEEVFPPERLAGMKIREKIRALVWKRLEIMGPAR
                     EAVRRGVAILALPQNLPLGLRIGWRTSDLMWRIAGDTSTDFNHYTKRMMLGAVYASTL
                     LAWLDDNSEGWSETAGFLDRRIDDVMRIEKWKAEWRGSGERPSVARFLGRLRYPPR"
     gene            complement(5704..6597)
                     /locus_tag="VFU20_03050"
     CDS             complement(5704..6597)
                     /locus_tag="VFU20_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5285473.1"
                     /translation="MAEPRSQPFAFAALLIGSCSLAFGPWLVRLAGVGPVAAGFWRLA
                     LALPFLWLIARMIGPPVHWPKRALVTLTVLAAFFFAADLAAWHTGIHMTKLGNATLFG
                     NVSSFAFAAWGLWLARRLPSPVQGAALFLAAVGAALLMGGSAELSARHLRGDLFALAA
                     GLLYTGYLIAVERARGQLDALPLLFLASAFGAAMLLPLSLALGERIVPADWTPVLLLA
                     LGSQVLGQGLLVYAIGALPPLVVGLALLTQPAISAFIGWVAYRETLSPLDWLGAAAIA
                     LALVLVRLPERGLRGAGQRPT"
     gene            complement(6629..7063)
                     /gene="ribH"
                     /locus_tag="VFU20_03055"
     CDS             complement(6629..7063)
                     /gene="ribH"
                     /locus_tag="VFU20_03055"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU5285474.1"
                     /translation="MARILIAEARSYAHLNDLLLAGARAAIEAAGHDHETVTVPGALE
                     IPAAIALAADSGRFGAYVALGVVIRGQTFHFEIVAQESARGILALTLDGLAVGNGILT
                     VEDEAQAIARADPKRIGKGGDKGGDAARAALALMELKRRFSV"
     gene            complement(7068..8351)
                     /gene="ribB"
                     /locus_tag="VFU20_03060"
     CDS             complement(7068..8351)
                     /gene="ribB"
                     /locus_tag="VFU20_03060"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HEU5285475.1"
                     /translation="MSTTLIERLSAVIATGEVSRAGLARAAGLHPNSLRKLGSPDWNP
                     TADTLGRLEKLLQRGNTNVLVGAEGIIDEARNGRMFILVDDDDRENEGDLVIPAQMAT
                     PDAINFMARHGRGLICLALTRERVEALGLQPMASQNRSRNNTAFTVSIEAKEGISTGI
                     SAADRARTVAVAIDAANGPDALVSPGHVFPLVAQPGGVLVRAGHTEAAVDVARLAGLN
                     PSGVICEIMREDGSMARLDDLMGFAAAHGLKIGTIRDLIAYRLKKDHMVERVASTGFT
                     SRSGAQWQAQVFRDKATGAEQLAMVHGTIEPDRPVLVRMHSLDLFADVLGESSERSGL
                     LSGAMAMIEAEGAGVIVALHAASPGSLSRAIDLRSGKPTEGGEALRNYGIGAQILAAL
                     GIHEMVLLTNTHHSPVALSGYGLAIVEERPIKLAD"
     gene            complement(8394..>8889)
                     /locus_tag="VFU20_03065"
     CDS             complement(8394..>8889)
                     /locus_tag="VFU20_03065"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014078005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU5285476.1"
                     /translation="CSGVCLTVVDKGDDWFAVDVSEETRSRTAKGLWSEGSRLNLERS
                     LRIGDELGGHIVAGHVDGVAEVIGACPEGGSSRIGLRAPDGLGPFIAPKGSITLDGVS
                     LTVNDVRDAEDGSTHFSVNIIPHTAQITTLGEIAAGRQLNVEIDVIARYLERMLASRA
                     HSAS"
BASE COUNT         1343 a         3084 c         3196 g         1266 t
ORIGIN      
        1 agttccgtcc tgagcgccca aagctcgggg aacgcccgct tgtccagcgt cgagcgcagg
       61 taagcggcgc cggccgagcc gccggtgccc ggcttgttgc cgatgatccg ctcgaccgtc
      121 agcacatgct tgtggcgcca catcgccagc gcgtcgtcga ggtcgaccag cttctcggcc
      181 agctggtaga ggtcgaacca gcgctccgcg tcgcgataga cctcgagcca ggcatcgccg
      241 accgccgctt ccgagcggtc gccgaggtcg aagccggcgc gcacgagcgc cgagtgcgag
      301 gagtcgcgca ggctcggctc gtcgagcgcc gagcgcagcc gggcccgcgc ctcgctgccc
      361 tgcgcataat ggtcgaggaa cttcgggtcc ttgatcccga gccggtattc gagctcgcgg
      421 aactgcaccg actgaaagcc cgacgaggtc ccgagcacgt cgcggaacgc actgtagtcg
      481 accggggtca gggtcgacag aacgtcccac gacagggtca tcaccgactg gatccggccg
      541 atccgcgcca tcgccttgta cgaggcggcg aaatggtcct ggtcgagcag ggcgatcgcc
      601 agcttgagct cgtgaagcat ctgcttcagc cacagctcct tggtctggtg gatgacgatg
      661 aacagcatct cgtcgtggag gcccgaaagc ggcttctggg cggtcagcag ccggtccagc
      721 gcgagatagt cggcataggt catgccggcg ggggtggcgg tcatggcggc aggcttacgg
      781 caaaggccgc aagctgccta gcaggagcct attgctggcg gccgctgccc gggagacccg
      841 gctggctgat ggtgccgatg ttgccgaaca gctcgtcgaa gaagctgcgc ttgcgcccca
      901 gcgtcggggt ccgggcgttg accgggtcga tcgaggcgat cagctccttg ccgctgcggt
      961 tgacggcggc gacgttgccg gcagcatcga agcggatgtg gagcacggtc tggtcgatca
     1021 caccgacgcc gcgcagggcg acgctcttgg tgtcgcgcga cacataatac cagtcgttgg
     1081 ggttgaactg gccggtgaag gtcgggcgcc ccagggtctt ggcgaccgaa tccttattgt
     1141 cgaccccgac ctggatcgcc gaggcgagct ccttgtcgat gaccgcgccg cgatgctcgc
     1201 ggatgcccgc gcatcccgcc agcaggaccg cacccgcaac cgccacacat gccttgaaca
     1261 acgcagcttt catgtttcgt gaacctctca tcgcccgcac tgggcgcgta aatgacgcta
     1321 acgcttgcgc cgcggccggg tgggctcaat atgcgccgga catgcagagg cgcaagcctt
     1381 gtgccaccgg agtccgagcg accggcatat gctgagcttc ctgttcccgc gattaacacc
     1441 gtccggcgcg agccgcggcg gcgccttgtt cgaggcgctg gtcggcgagg cccggcggcc
     1501 gcactggtat gtcgaggggc aggtccccga cacgctcgac ggccgcttcg cggtgcttgc
     1561 gacgctggtc gctctggcga ccgtccggct cgagcgcggc ggcgattcgg cgcgggccga
     1621 ggaagcggcg ctcgccgagc ggttcgtcga ggcgatggat tccgagcacc gcgagctcgg
     1681 gctcggcgac ccggcggtcg gaaagacggt gcgccggctg gtcggggcgc tcgcccggcg
     1741 cgtcgaccga tcgcgggcag cggtcgagcg cggcgaccaa tgggacgatg cggtaaccga
     1801 cagcctgttc cgcgactcgc ccccggccga tgctctcgcg cattgcgcgg cggccctgcg
     1861 cacaatctgg gcgaggctcg aagcggcttc cgacgaggcg ctcgcgcaag gcaggatcgg
     1921 atgatcgagg acttcgccca ccggctgacg ctcgaccgga tccgcgacgg cgagcgcatc
     1981 gacctcgtcg ccgacgacga ggagcgccgg gcgatcgccg atcggctcga cctgccgtcc
     2041 ctggaccggc tcgaggcgca cgccagcctg gcgcgcgacg gcgacacggt gcgcgccgcc
     2101 ggccggatcg ccgcctcgct cgagcagcgc tgcgtggtca ccggcgagcc ggtcgcggcg
     2161 cacgtcgacg agccgttcga catcgccttc gttcccgagc cgcgcgccgg cgacgtgccc
     2221 gaggaggtcg agcttcgccc cgaggattgc gacaccgtgt tccacgacgg ctcggcgatc
     2281 gacctcggga cggcgatcgc cgacaccctg gcgctcgccc tcgatcccta tccgaggagc
     2341 gcgtcggccg aagccgcgct cagggaggcc ggcgtgctga ccgaagccga agccggcccg
     2401 ttcgccgagc tcgccaagct caagaaaggg ctgggcggcg gcgacgccta gacaggcccg
     2461 ccccagtcga caggccccgc cctagaacgg gacgtcgtcg tcgaggtcgc tgtcgaacgc
     2521 ggcgggttgc gggcgggatt gcgtgcgcgt gccgccgccc gagcttgcgc cgccggcgaa
     2581 ctcatcggcg aaatcgccgc cgccgccgcc gccgccgccg cgcgagccgc caccctcgcc
     2641 gcggctgtcg agcagcacca gctcgccgcg gaagcgctgc aggacgatct cggtcgaata
     2701 gcggtcggcg cccgactggt cctgccattt gcgggtctgg atctgcccct cgagatagac
     2761 cttcgagccc ttgcgcagat agtttttggc gactcggccg aggttctcgt tgaagatcac
     2821 cacccggtgc cactcggtgc gctcctggcg gttgccgtcc ttgtccttcc actgctcgga
     2881 cgtcgcgacg cgcaggttga cgacctcgcc gccgctgttg agcgagcgcg cctccgggtc
     2941 gtcgccgagg ttgccgacca ggatcacctt gttcacgccc gccattgccg tttccttcgt
     3001 ctttcgcgct gaaatgcgac gatagaaccg cgctcagacg acagcaagcg gccggctgga
     3061 ccggcggttc cagccgaagc cgagcagcag gacgagcagg gtcgcggcct gggcggcaag
     3121 gccctcgagc gtcgggtaga ggccgaggat cgcgacccgc ggcgccgtgg cgaccggagt
     3181 gaccccgagc agccccgcct cctgaagcgc ggcgacgccc ttgccggcca gcaccaccgc
     3241 cagcaccgcg atcagagccg agctgtaggc gaagaacttg ccgatcggca gccgcgcgct
     3301 gaagcggaac atggcgaggg cgatggctgc gagcaggacc agcccggccg ctccgccggc
     3361 cgccagagcg ccgatattgc cctccgccga cagcgcggcg aagaacaata tggtctcgaa
     3421 cacctctcgg tagacggcga tgaaggcgag caggaacagg aaccaggccg aacgcccgga
     3481 cagcgcggcg ccgagccgcg ccttgatgta gcgctgccat tcgtcggcct gcgccttgcc
     3541 gtgcatccag atgccgacga acagcaggat cagggcggcg aggatcgagc cgaatccctc
     3601 ggtcagctcg cggctggcgc cgctgatggt cagcacgtgc gtcgccgccc accaggtgcc
     3661 gaggccggcg accagcgccg cgacccagcc gtaatgcacg gtgcggacca tctccggccg
     3721 ctcggccttg cgcaggaagg tcagcatggc gacgacgatc agaagcgcct cgagcccttc
     3781 gcgaagcagg atggtgaagg cgccgacaaa ggccgacgcc gcgccggcct cgcccggggc
     3841 gagcacgcgc tcggtgtcgg cgaacatggc gtcgagctcg gccgcgcgct ggcggacctc
     3901 ggcggccgga cggccggcgc cgatcgcggc gcgcagctgc gccattgccc gctcgacccg
     3961 cgccatcagc gccgaatcac gcgccgccag caccggctcg atcggctcga acccgtcgag
     4021 ataggcggag agggccagct gcttggcgcg ctcgcggtcg ccggcctcgt aggcggcgag
     4081 gctctgccgc agccggtcgc gggcgagcgc caggctgttg gaatcggccg gagcgacgac
     4141 ggcttcggga ttggcccgca ggtaagcgat gacggcggcc gccctggact cgccgatcgc
     4201 gcgcgcgagc tcggccgggg tcaggccggc cagcgccgcg aggtcgggga tgcgttggcg
     4261 gagcgtcggg tcggattccc acagacgctt gccttcggcg gcgagcgctt ccgggtaggc
     4321 gaagcggccg gagtggaagg cgagcgccca tttgtcggct tcgggcaaat ggcggaagct
     4381 cgccatcgcc gttccctcga ggccctggtc gatcacctgg tagagcgcga acgggctgcg
     4441 ctcgccggct cgggcgcggt cggtgaaggc gatcggcggc gggtccatgg tcgcggcaac
     4501 cggcaccttg gcgtcgccgt tggcgccgtg gcacgcggcg caattgtcgg cgtagagctt
     4561 cgctccgcgg gcgacgtcgg gcacctgggc cggcgcgagg gggacggggt aggcggcgag
     4621 cagctgcgag gcgagcgacc gcgcgtgccg gtcgatcgcc tcgggcgggg atttggccgc
     4681 gatcgccttg cgaagcgccg ccgcgccttc gaccagctgc ggcttgccgg gtttgtccgg
     4741 gagcgcctcc agccgctcgc cgaccgaggc cgagaattcg accatctcgt catattcgag
     4801 ccggttgacg atgcgcccgc ccgcgaccgc ctcgcgatag tcgacggcga tgtagtcgag
     4861 cagccgccac gcggtctgca cgtcctgcga ctgagccacg gccggcgcca cgggaagcgc
     4921 cagcgcggcg agcgcgatca gcatccagaa acgcttgagc agggccatgg agcggcagtc
     4981 atccccggtc gtgtgagggc ccgcggctag cctaattgca actgattatc aataacggtt
     5041 gggcggctag cgcggcgggt agcggagccg gccgaggaag cgcgcgacgc tggggcgctc
     5101 gcccgagccg cgccactcgg ccttccattt ctcgattctc atcacgtcgt cgatacgccg
     5161 gtcgaggaag ccggcggtct cgctccagcc ttcgctattg tcgtcgagcc aggccagcag
     5221 cgtcgatgcg tagaccgcgc cgagcatcat gcgcttggtg taatggttga agtcggtgct
     5281 ggtgtcgccg gcgatccgcc acatcaggtc ggacgtgcgc cagccgatgc gcaacccgag
     5341 cggcaggttc tgcggcaggg cgaggatcgc gactccgcgg cgcacggctt cccgcgccgg
     5401 gcccatgatc tcgagccgct tccacaccag cgcgcggatc ttctcgcgga tcttcatgcc
     5461 cgcgagccgc tcgggcggaa acacctcctc gagcgcccgg tcgacgccct ggatgtagac
     5521 gtcgaccatc cccgcctggg tcttgggcat cgccaggcgc gcctgggcgc ggtcgatacc
     5581 caggctgtcg gcggccgcgt cgatcgccgc gctcgaccag ccgtcgaaca ccgcatgctc
     5641 gccgaccgcc agcgccagac gctggcgcag ctgctcgaga ggagtcggct gttccatcgg
     5701 cggctaggtg ggccgctggc ccgcgccgcg caagccccgc tccgggagcc ggaccagcac
     5761 cagcgccagg gcgatcgccg cggccccgag ccagtcgagc ggcgacaggg tctcgcgata
     5821 ggcgacccag ccgatgaagg ccgagatcgc cggctgggtg agcagcgcga ggccgaccac
     5881 cagcggcggc agcgcgccga tcgcatagac cagcagcccc tgcccgagca cctggctgcc
     5941 gagcgccagc agcagcacgg gggtccagtc ggcggggacg atgcgttcgc ccaaggccag
     6001 cgacaaaggc agcagcatcg ccgccccgaa cgcgctcgcc aggaacagca agggcaaggc
     6061 atcgagctgg ccccgcgcgc gctcgaccgc gatcagatag ccggtgtaga gcaggcccgc
     6121 ggcgagcgcg aacaggtcgc cgcgcaggtg gcgcgcggac agctcggcgc tgccgcccat
     6181 cagcagcgcc gctccgaccg ccgcgaggaa gagcgccgct ccctggaccg gcgacggcag
     6241 ccgccgcgcc agccacaggc cccaggcggc gaaggcgaag ctcgagacgt tgccgaacag
     6301 ggtcgcgttg ccgagcttgg tcatgtggat gccggtgtgc caggcggcga ggtcggcggc
     6361 gaagaagaat gcggcgagga ccgtcagggt gaccagcgcg cgctttggcc agtggaccgg
     6421 cggcccgatc atccgggcga tcagccacag gaaggggagc gccagtgcga ggcgccagaa
     6481 gcccgcggcg accggcccca ccccggccag ccgcaccagc cacggcccga acgccagcga
     6541 gcaactgccg atcagcaatg cggcgaaggc gaagggctgg gatctgggtt cggccacgca
     6601 ctgcgcttag cgtggagtcg cgctgctgtc agacggagaa tcgccgcttc aattccatca
     6661 gcgcgagcgc ggcgcgggcc gcgtcgccgc ccttgtcgcc ccccttgcca atgcgcttcg
     6721 ggtcggcgcg ggcgatcgct tgcgcttcgt cctcgacggt caggatgccg ttgccgacgg
     6781 ccagcccgtc cagggtcagc gccaggatcc cgcgggcgct ctcctgcgcg acgatctcga
     6841 aatggaaggt ttgcccacgg atcacgaccc ccagcgcgac ataagcgccg aagcggccgc
     6901 tgtcggcggc gagcgcgatc gccgccggaa tctcgagcgc tccggggact gtgacggtct
     6961 cgtgatcatg gcccgccgcc tcgattgcgg cccgggcgcc ggccagcagc aaatcgttga
     7021 gatgggcgta agagcgcgcc tcggcgatta gaattcgcgc catgtcgtca gtcggcgagc
     7081 ttgatcgggc gttcctcgac gatcgcgagg ccgtagccgg acagcgccac cggcgaatgg
     7141 tgggtgttgg tcagcagcac catctcgtgg attcccagcg cggcgaggat ctgggcgccg
     7201 ataccgtaat tgcgcagcgc ctcgccgcct tcggttggct tgcccgagcg caggtcgatc
     7261 gcccgcgaca gcgagcccgg cgacgcggcg tgaagcgcga cgatcacgcc cgcgccttcg
     7321 gcctcgatca tcgccatcgc cccggaaagc aggcccgagc gctcgctcga ttcgccgagc
     7381 acgtcggcga acaggtcgag gctgtgcatg cgcaccagca ccggccggtc gggctcgatg
     7441 gtgccgtgga ccatcgccag ctgctcggcg ccggtcgcct tgtcgcggaa cacctgcgcc
     7501 tgccattggg cgccgctgcg cgacgtgaag ccggtcgagg cgacccgctc gaccatatgg
     7561 tccttcttca ggcggtaggc gatcaggtcg cggatggtgc cgatcttgag gccgtgggcg
     7621 gcggcgaagc ccatcagatc gtcgagccgg gccatgcttc cgtcctcgcg catgatctcg
     7681 cagatcacgc ccgacgggtt gagcccggcg agtcgggcga cgtcgaccgc ggcctcggtg
     7741 tggccggcgc ggaccagcac gccgccgggc tgggcgacca gcgggaacac gtggccgggc
     7801 gagaccaggg cgtcggggcc gttggcggcg tcgatcgcca ccgcgacggt gcgcgcccgg
     7861 tcggcggcgc tgatcccggt cgaaatcccc tctttggcct cgatcgagac ggtgaaggcg
     7921 gtgttgttgc ggctgcggtt ctggctcgcc atcggctgca gcccgagcgc ctcgacccgc
     7981 tccctggtca gcgccaggca gatcagcccg cggccgtggc gggccatgaa gttgatcgcg
     8041 tcgggggtcg ccatctgcgc ggggatcacg aggtcgccct cattctcacg gtcgtcgtcg
     8101 tcgacgagga tgaacatccg cccgttgcgc gcctcgtcga tgatgccttc cgcgccgacc
     8161 agcacgttgg tgttgccgcg ctggagcagc ttctcgagcc ggccgagcgt gtccgcggtc
     8221 gggttccagt cgggcgagcc cagcttgcgc aggctgttgg ggtggagccc ggcggcccgg
     8281 gcgaggccgg cgcggctgac ctcgccggtg gcgatgacgg cgctgagccg ctcgatcagg
     8341 gtggtggaca tgattgcccg atatcacact gcaatgtgat cgcaagccaa agtttacgac
     8401 gcggaatgtg cgcggctcgc cagcattcgc tcgagatagc gggcgatcac gtcgatctcg
     8461 acattcaact gtcgcccggc cgcgatctcg ccgagcgttg tgatctgcgc ggtgtggggg
     8521 atgatgttga ccgagaaatg ggtcgagccg tcctcggcgt cgcgcacgtc gttgacggtc
     8581 agcgacactc cgtcgagcgt gatcgagccc ttgggggcga tgaacggacc gaggccgtcg
     8641 ggcgctcgaa gcccgatccg cgacgagccg ccctcggggc acgcgccgat cacctcggcg
     8701 accccgtcga cgtggccggc gacgatatgg ccgccgagct cgtcgccgat gcgcagcgag
     8761 cgctcgaggt tcagccggct gccctcgctc cacaggccct tggcggtgcg cgagcgggtc
     8821 tcttccgaga cgtcgaccgc gaaccagtcg tcgcccttgt cgaccacggt caggcagacg
     8881 ccagagcag
//
LOCUS       DASTZO010000109         2375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_922711,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000109 DASTZO010000000
VERSION     DASTZO010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922711"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..>2375)
                     /locus_tag="VFU20_03070"
     CDS             complement(96..>2375)
                     /locus_tag="VFU20_03070"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5285477.1"
                     /translation="LDVPESAGAAPGDEHRAVGGAGVIGTQLGHYKILSQLGRGGMAS
                     VYKAEQAGLGRLVALKILQPSLAADEIIVQRFQQEARIAANLHHAHVVTIYDVGAIDG
                     VYYISMRFIDGENLGQLLRREGPLEPQRALRLLEQVAEALDFAHSRNILHRDIKPANV
                     MVEPGDVLTLTDFGIARAGEQSHLTATHMVIGTPEYMSPEQARGDMVDRRSDLYALGV
                     LLYEMLGGRPPFSAASTPSLLYLHVHEPPPPLYQLRPDLPRPLSEVVTKSLAKDPNER
                     YQSGREMVAAARAALAQPSPTVVESPPTAGLTSATVVDPNFGRGGWPPPGTPPPGAHP
                     PSGRPAPGTPPPGARPTPMPIRPTPPPPRTGGQGVRMEPAAHANATRTLTPPPIPSAV
                     DRGAYSARPVVQPTPAPRPDRQPKRSSTSIVGPILAVVALLAVVVLVLTQTGVMDRVI
                     GTAPPTAIVDKPAAVVTPTPVPVAATNPKPASGPTPTPGITPTPAEAAVKPADKPAEK
                     PAEKPAEKPSQPPTNTPVPVPPTPNPEQRVASAQAAVAAGDLPTAIDQLRVVRDDPSV
                     QANPQLVQQVNDTLRKAHVTHGNQLLEQNKIDDSYAQFAEALKIASNDPEALEGQKRV
                     ILTKNYAAMEAAWGKDDDAAIKALEENMLIDAGFRETRAKLYSLLIGKADRMLETGDR
                     DGAFPVLMRALEVSPEPGEAQRRLAAYTPTPVPPTPVPARPAPPPYQPQPQPQPRPQP
                     QPQPQPQPERPFRPPGAPV"
BASE COUNT          299 a          791 c          877 g          408 t
ORIGIN      
        1 gagctcgggg aacgaagacc ttcgcgtgcc tccgctagca cttgcctcgg cgagcaaggg
       61 aaccgcgcga acgtccgaac ggtgtgaccg gaatcctaga cgggcgcgcc cggcggtctg
      121 aacggccgct ccggttgcgg ctggggctgc ggctgcggct gcggtcttgg ttgtggctgc
      181 ggctgcggct gatacggtgg cggggccggg cgggcgggca ccggcgtcgg cgggaccggc
      241 gtcggcgtgt acgcggccag tcgccgctgg gcctcgccag gctccggcga aacctccagg
      301 gcgcgcatca gcaccgggaa ggcgccatcg cggtcacctg tttcgagcat tcgatccgcc
      361 ttgccgatca gcagcgagta caacttggcg cgcgtctcgc ggaatccggc gtcgatgagc
      421 atgttctctt cgagcgcctt gatcgccgcg tcgtcgtcct tgccccaggc cgcttccatc
      481 gcggcgtagt tcttggtgag gatcacccgc ttctggccct cgagggcctc gggatcgttc
      541 gacgcgatct tgagcgcttc ggcgaactgc gcgtagctgt cgtcgatctt gttctgctcc
      601 agcagctgat tgccgtgggt tacgtgcgcc ttgcggagcg tgtcgttcac ctgctgcacg
      661 agctgcggat ttgcctgcac ggacggatcg tcgcgcacca cccgcaattg atcgatagcg
      721 gtaggcaggt cgcccgccgc gacggctgcc tgcgcgctgg cgacccgctg ctcgggattc
      781 ggtgtcggcg ggaccggcac cggcgtgttc gtcggcggct gcgacggctt ttcggctggt
      841 ttctcagccg gtttttcagc gggcttgtcg gctggcttga ctgccgcctc ggccggtgtg
      901 ggcgtgatgc ccggcgtcgg ggtcggcccg ctggccggtt tcggattggt cgcggcgaca
      961 gggactggtg tcggcgtgac gacggccgcc ggcttgtcca cgatggccgt tgggggcgcc
     1021 gtcccgatga cccggtccat cacgccggtc tgggtcagga cgaggacgac taccgcgagc
     1081 agcgctacga ccgccaggat cggcccgacg atcgacgtgg aggagcgctt cggctgtcgg
     1141 tccggcctcg gcgctggcgt cggctgaacg accggccggg cgctgtatgc gccgcgatcc
     1201 actgccgacg ggatcggcgg cggggtcagg gtgcgggtgg cgttggcgtg ggcggccggc
     1261 tccatccgga ccccctggcc gcccgtgcgc ggtggcggcg gcgtcggccg gatcggcatt
     1321 ggcgtcggcc gggcgcccgg cggcggggtg cccggcgcag gccggccgct cgggggatgg
     1381 gcgcctgggg gcggagttcc gggcgggggc cacccgccac gcccaaagtt cgggtctacc
     1441 acggtggccg aagtcaggcc agccgtcggc gggctctcga cgacggtcgg cgagggctgg
     1501 gccagcgccg cgcgggcggc agccaccatc tcgcgcccgc tctgatagcg ctcgttcggg
     1561 tctttcgcca atgacttcgt gacgacctcg ctcaacgggc ggggcaggtc cggacgaagc
     1621 tgatagagag gcggcggcgg ctcgtggacg tgcaggtaca gcagcgaagg cgtgctggcg
     1681 gcgctgaatg gcggcctgcc gcccagcatc tcgtacagca gcaccccgag cgcgtacagg
     1741 tcggatcggc ggtccaccat gtcgcctctg gcctgctccg gcgacatgta ctctggcgtg
     1801 ccgatgacca tatgggtcgc ggtcagatgg ctctgctcgc cggcccgcgc gatgccgaag
     1861 tccgtcagcg tcagcacgtc ccccggctcg accatcacgt tggccggctt gatgtcgcgg
     1921 tggaggatgt tgcgcgaatg cgcgaagtcc aacgcttcgg ccacctgctc cagcagtcga
     1981 agggcgcgct gcggctccag cggaccctcg cggcgcaaca gctgcccgag attctcgccg
     2041 tcgatgaacc gcatcgagat gtagtagacg ccgtcgatcg cgccgacgtc gtagatcgtg
     2101 accacgtggg cgtgatggag gttcgcggcg atgcgcgcct cttgctggaa acgctgaacg
     2161 atgatctcgt cggctgccag cgacggctgc aggatcttga gcgcgacgag ccgtcccagg
     2221 ccagcttgct cggccttgta gaccgaggcc atccccccgc gcccgagctg gctcaggatc
     2281 ttgtaatgcc cgagctgtgt gccgatcacc ccggcgcctc caacggctcg atgctcgtcc
     2341 cctggcgccg cgcccgccga ttcgggcaca tccaa
//
LOCUS       DASTZO010000110         2674 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_870429,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000110 DASTZO010000000
VERSION     DASTZO010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_870429"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VFU20_03075"
     CDS             <1..493
                     /locus_tag="VFU20_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5285478.1"
                     /translation="HVRRGEVVLLDCGCEVRGYQSDISRTFVFGAKPTAEQRRVWTQM
                     RRGQDIAMAAARPGAAAGSVDDAVRRTYESWGYGPGYRLPGLSHRTGHGIGMEGHEPV
                     NLVHGETTRLAPGMCFSNEPGIYLPGKFGIRLEDCFHMTAAGPKWFSAPPPSIDDPFG
                     RSA"
     gene            567..1898
                     /locus_tag="VFU20_03080"
     CDS             567..1898
                     /locus_tag="VFU20_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEU5285479.1"
                     /translation="MARKPKPEKRSLDLQWQMLIGFLVGLVAGLIVYSTQPDAEWVKV
                     VTTYVTQPIGQIFLRLLFMLVIPLLFSALIVGISEMGEVRALKRVGLRTLAYTVIVSS
                     IAVIVSLAVVNLLKPGAGVDRAVAASLLAAQEGRAGEIVQTGREQPTGVDAFVNIIPN
                     NLVEVMGSNSSILSVMFFALFFGIGLLITDTPNSRALKRGFEGLFDVTMRLIMIVIRL
                     APIAVACFMFNLAALFGWDLLIRLSAYVGVVLLALAIQMFIVFPALLATLGRKNPIQF
                     FRETQEASLMAFSTASSNATLPTSLRVADEKLKLPRRVSRFVLTIGATANQNGTAMFE
                     GVTVIFLAQFFGVELTVWQQVMVMLVCILGGIGTAGVPAGSLPVVALILAMVGIRPEA
                     IALVLGVDRFLDMCRTTLNVIGDLVAAQVISSAEPHEGEPVDAVPRAPQPA"
     gene            1895..>2674
                     /locus_tag="VFU20_03085"
     CDS             1895..>2674
                     /locus_tag="VFU20_03085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEU5285480.1"
                     /translation="MRDAPATEPGVLEFGRDILHDEARALDALADSLGEPFERAIAMI
                     LAMPGKLIVSGLGKSGHIGRKIAATFASTGTTATFLHLAEAIHGDMGIAAKGDVALLI
                     SQSGTTAELEAVIDHFSAVGIPIVSITGNARSMLAEASAAPLVLPHWSEVGPEAVAPT
                     TSTTMTLALGDALAMTVMRHKGFTRTDFGRLHPGGSLGARLKPVRRLMHGGDAMPLTR
                     RDTSMHQAVVEMTEKRLGVVGVTDDDGNLVGIITDGDLRRNI"
BASE COUNT          400 a          930 c          895 g          449 t
ORIGIN      
        1 gcacgtccgg cgcggcgagg tcgtgctgct ggactgcggc tgcgaggtgc gcggctatca
       61 gtcggacatc tcgcgcacct tcgtgttcgg tgcgaaaccg accgccgagc agcgccgggt
      121 gtggacccag atgcggcgcg gccaagacat cgccatggcc gcggcccggc ccggagcagc
      181 cgcgggcagc gtcgatgacg ccgtgcgccg gacctacgaa agctggggct acggcccggg
      241 ctacaggctg ccgggcctgt cgcaccgcac cggccacggc atcggcatgg aggggcacga
      301 gccggtcaac ctggtccacg gcgaaaccac ccgcctcgcg cccggcatgt gcttttccaa
      361 cgagcccggc atctatctgc ccggcaagtt cggaatccgg ctcgaggatt gcttccacat
      421 gaccgcggcc gggccgaaat ggttctcggc gccgccgccc tcgatcgacg atccgttcgg
      481 gcgctcggct tgactttgcc aagcgccgct tgacctttcg ctccccgccc gcgaatccgg
      541 tgcggcacaa gaggggacga cgaaacatgg ccaggaagcc gaagcccgaa aagcgcagtc
      601 tcgacctgca atggcagatg ctgatcggct tcctggtcgg actcgtcgcc ggcctgatcg
      661 tctattcgac ccagcccgac gccgagtggg tcaaggtcgt caccacttat gtgacccagc
      721 cgatcggcca gatcttcctg cgcctgctgt tcatgctggt gatcccgctg ctgttctcgg
      781 cgctgatcgt cggcatttcg gaaatgggcg aggtgcgcgc attgaagcgc gtcggcctca
      841 ggacgctcgc ctacacggtg atcgtctcgt cgatcgcggt gatcgtcagc ctcgcggtgg
      901 tcaacctgct caagccggga gccggcgtcg accgcgcggt cgccgcgtcg ctgctcgccg
      961 cccaggaggg acgggcgggc gagatcgtcc agaccggccg cgagcagccg accggggtcg
     1021 acgcgttcgt caacatcatc cccaacaacc tcgtcgaggt gatgggcagc aacagctcga
     1081 tcctgtcggt gatgttcttc gctcttttct tcggcattgg cctgttgatc accgacacgc
     1141 ccaattcgcg ggcgttgaag cgcggcttcg aggggctgtt cgacgtcacc atgcggctga
     1201 tcatgatcgt catccgcctg gcgccgatcg cggtcgcctg cttcatgttc aacctcgcgg
     1261 ccctgttcgg ctgggacctg ctgatccgac tgtcggccta tgtcggggtc gtcctgctgg
     1321 cgctggcgat ccagatgttc atcgtcttcc cggcgctgct cgcgacgctc ggccgcaaga
     1381 acccgatcca gttcttccgc gagacgcagg aggcgagcct gatggctttc tccaccgcct
     1441 cgtccaacgc gactctgccg acctcgctcc gagtcgccga cgagaagctg aagctgccgc
     1501 ggcgggtgtc gcgcttcgtt ctcaccatcg gcgcgaccgc caaccagaac ggcaccgcga
     1561 tgttcgaggg cgtgacggtg atcttcctcg cccagttctt cggggtcgag ctgaccgtct
     1621 ggcagcaggt gatggtgatg ctggtctgca tcctcggcgg aatcggcacc gcgggggtgc
     1681 ccgcgggatc gctgccggtg gtcgcgctga tcctggcgat ggtcgggatc cgccccgagg
     1741 cgatcgcctt ggtgctcggc gtcgaccgct tcctcgacat gtgccggacg accctcaacg
     1801 tgatcggtga cctggtcgcg gcccaggtca tttcctcggc cgagccgcac gagggcgagc
     1861 cggtcgacgc ggtgccgcgg gcgccgcagc cggcgtgagg gacgcgccgg caaccgagcc
     1921 cggagtgctc gagttcggcc gcgacatcct ccacgacgag gcgcgggcgc tcgacgcact
     1981 cgccgacagc ctcggcgagc cgttcgagcg ggcgatcgcg atgatcctcg ccatgccggg
     2041 caagctgatc gtcagcggac tcggcaaatc gggccacatc ggccgcaaga tcgccgcgac
     2101 cttcgcctcg accggcacca ccgcgacctt cctccatctc gccgaggcga tccacggcga
     2161 catgggcatc gccgccaagg gcgatgtcgc gctgctgatc tcgcagagcg gcacgaccgc
     2221 cgagctcgag gcggtgatcg accatttctc ggcggtcgga atcccgatcg tctcgatcac
     2281 cggcaacgcg cgctcgatgc tggccgaggc gtccgcggcg ccgctggtgc tgccccactg
     2341 gtccgaggtc gggcccgaag cggtcgcccc gaccacctcg acgaccatga ccctggcatt
     2401 gggcgacgcg ctggcgatga cggtgatgcg ccacaagggc ttcacccgca ccgacttcgg
     2461 ccggctgcac ccgggcggct cgctcggagc gcgtttgaag ccggtgcggc ggctgatgca
     2521 cggcggcgac gccatgccgc tcacccgtcg cgacacgtcg atgcaccagg cggtggtcga
     2581 gatgacggaa aagcggcttg gagtcgtcgg ggtcaccgac gacgacggca atctggtcgg
     2641 catcatcacc gacggcgact tgcgccgcaa catc
//
LOCUS       DASTZO010000111        10989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_478776,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000111 DASTZO010000000
VERSION     DASTZO010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 10989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10989
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478776"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /gene="pip"
                     /locus_tag="VFU20_03090"
     CDS             <1..645
                     /gene="pip"
                     /locus_tag="VFU20_03090"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HEU5285481.1"
                     /translation="VDKWQVFGGSWGSTLSLAYAITHPERVTELVLRGIFLFGQSELD
                     WLYKYGASELFPEGWDAFVSLIPEDERGDLIEAYRKRLTGDDPAERLAAAKAWSTWEG
                     LTVTLLPDPKMLEEFTEDQHAIAIARIENHYMANKGWLEDGQLLRGADKLRSIPGVIV
                     QGRHDCCTPPRAAWELKQAWPEVELQIVPDGGHLYSEPGVLDGLIRATDRFASR"
     gene            757..3066
                     /locus_tag="VFU20_03095"
     CDS             757..3066
                     /locus_tag="VFU20_03095"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5285482.1"
                     /translation="MRRILCSTSLYALAAAAALSAAPAAAQATGDTDTPAADAQQADS
                     QQGVGEIVVTAQRRTENLQDVPLSVATIADETLAAINSGGADVRGLSGRVPSLSIESS
                     FGRTFPRFYIRGLGNTDFDLNASQPVSLVYDDVVLENPILKGFPAFDVDRVEVLRGPQ
                     GTLFGRNTPAGIVKFDTVKPGRGRNFARVSYGTYGTINAEGAVGGKLGEGAAARLSGI
                     WQHRNDWIDNLDDPGGNDLEGYDDFAVRGQVEFQPTEALTVRLTGQLRDLDGDARIFR
                     ANAIEFGSNDLIGVDGGGFERDEVRHDAINFQKLKTQNIAGAIEYDFGAATLYSVTGY
                     WHGKLDSRGDIDGGFGCSFCGPGFTDNSEPGFIPFAAQSQDNIPSLDQFTQEIRIASN
                     NRGGLGFQAGIFYFNEKLDIESFDFATPTAPTPSAIVNQRQDSDAVGIFGSVNYAFAS
                     GLTLQAGARWNHDKKKLTADRLFDTRFIFIGGGPVPESTTKVKDSVLTWDLSALQEIN
                     PDVNVYARIARGYRAPAIQGRILFDRDVTTADSERTMSYEAGVKTVLLDRRLRFNLTG
                     YHFRTKDLQLSAVGGGTNANLLLNADAVDGYGFEAELQARPARGLTLTGGLSYNHTKI
                     DDDNLTVETCGAPCTVLDPVVVPAGLFQPAIVSIDGNALPQAPRWIANFTAGYEHPVG
                     PGSVYVFTDWYHRSKIQFFLYRSVEFSDDQLLEGGLRIGYKTERFDVAGFVRNITNDE
                     SAVSGIDFNNLTAMVNEPRIWGVEVGVKF"
     gene            complement(3107..3994)
                     /locus_tag="VFU20_03100"
     CDS             complement(3107..3994)
                     /locus_tag="VFU20_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285483.1"
                     /translation="MNRFAAACVLLTLATSAPAQKGKPTTPLTPEIAYTYASGNYMDL
                     RLANEDGTGAVTLHRGAYGAITQFDLGPRSQMQVAFLENRDLKLLTYEPTGTGNVRTS
                     GVVTLYAGPGNVDSLSFSLDGSKIAIFVQEQDNNRILALDTANGAVTELGKVNWAFYL
                     AWYHDGSGVVYKDLDQATSQYVVRELLLDGANQLLLSEANIEHVDTARTSKDLLVSYS
                     RPGLDEIRVGRWSNGNYSNERVVAGLLAHFNCNDTRFIYRGYARMKRPTYKYSYATNS
                     NSTFSTDNDIHHTDWMPTC"
     gene            complement(4129..6618)
                     /gene="ligD"
                     /locus_tag="VFU20_03105"
     CDS             complement(4129..6618)
                     /gene="ligD"
                     /locus_tag="VFU20_03105"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HEU5285484.1"
                     /translation="MARRAALDIETYNRKRDFSKTAEPKGRKLKGKGDSFVVQKHDAS
                     RLHWDFRLELDGVLKSWAVPKGPSLDPAEKRLAMRTEDHPLDYGGFEGTIPAGEYGGG
                     TVMLWDRGRWVPEPGKDPKKTIEDGHLHFTLEGERMKGEWVMFRLKPRPGEKAEPWML
                     KKVTDEFADEEAGDALVDECVTSVTTGRTMAEIASGGDVWRSKRGGRKGGRAKKKSGQ
                     GPPPFREPQLATLVDSVPAGSDWIHEYKYDGYRLLIATGDGAATAWTRKGKDWSDKFR
                     ALVRAAAKLPPGCLIDGEAVALNDQGKPDFQLLQSTLKEHKGANLAFYAFDLLVDRGD
                     DIVKLPNLERKERLAALLKGVGPPIIYGDHVVAKGEALFNAICAEGGEGIISKKASAP
                     YRGTRTRCWLKVKCIQRQEFVVVGWSESDKRRGFRSLLLGVRDGRKLTYAGKVGTGFN
                     TRMIDDLMEKMIPLEVDKAPLEVPRADRKAAHWIEPKLVAEIAYTEFTSDGILRHPSF
                     IALREDKPAAEVVREVPQKLAKSEKKKGKRPAAEDFGIAISNPDRVIYPEGRLTKKDL
                     ADYYAQIEALIMVDAGRRPMTLIRCPQGRGKHCFFQKHDTGSMGEHVKHVPIKEKKGE
                     TQDYLYFDDVRGLLACVQMGTIEFHGWGSRVDKVEHPDRLVFDLDPDVGLDFAAVRSA
                     AVRLRELLLDLGLTSFPLLTGGKGMHVVVPLDAKADWAAVKSFAERFSRAVSEAEPER
                     FTANIRKAQRKGRIFLDWLRNQRGATAVLPYSARAREGAPVAAPIAWEEIDEYKGGNA
                     FSIRDADTLIDRASSKLLAGWGQAKQALPKA"
     gene            complement(6622..7527)
                     /locus_tag="VFU20_03110"
     CDS             complement(6622..7527)
                     /locus_tag="VFU20_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HEU5285485.1"
                     /translation="MAARPIWRGQIRLALVSIPVEIYPATRSGATVQFHQVHEPSGKR
                     IKYEKVVPGIGPVDRDEIMKGYEVSKGHYVLLDPEEIESVKLESRKTVDLVQFVDLGD
                     IDPMYFDKPYYVVPTDDLAEEAFVVLRDALREARKVGIGQLAMRGQEYVVALKPCGRG
                     MLLETLRYADEVNRAASFFREIGDHKPDPDLLDLAATLIEKKTGDFDPKEFHNRYVDA
                     LKNLIKEKQRKKGEKVIQDPDADAPPSRGSNVIDLMAALKKSLGETGGATGDRRGPAR
                     KPAARKAPARKAAAPARKTAARKRA"
     gene            complement(7640..9148)
                     /locus_tag="VFU20_03115"
     CDS             complement(7640..9148)
                     /locus_tag="VFU20_03115"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5285486.1"
                     /translation="MPIKSVLAPILDTVLDAVVVMDRDGVIRAWNQYAQATFGWSASE
                     AIGQNLGDLIVPPSLREAHQRGLRRFNSEGIAKVLDKRLELSGLRRDGSEVPVELSIT
                     LVSRDGRDMFVGFLRDISERKRAERQLEFQVRESRLMLELSDLASRDKSFEEALVAIL
                     DGICELADWPVGHAFLVGEDQRTLKEGSWSSKAKKTAPALVKATAAIHFEPGVGLPGR
                     VLESGKPLWISSVNADDNFMRAGLGFESAFGFPVHSGGRCIAVLEFFSRDSRQPEEHL
                     LLSARAIGAQVGRVHERIRSEELRALLLAELNHRAKNILAVVRGMAHLSFGSASDVAE
                     AQKAFDRRLDSIAKANDILHAQSGSSALLSDIIREALAGCGVGEDRASLSGPDLCIDS
                     STSIMVSLAVHELSTNAFKYGALSVRGGSIAIAWRANPENDARFDFEWTEAGGPPVEA
                     PKRKGFGMRILERGIQLETGGRPQVSYDPSGFRYRLTGARHSGVPGDRAKAA"
     gene            complement(9365..10168)
                     /gene="trpA"
                     /locus_tag="VFU20_03120"
     CDS             complement(9365..10168)
                     /gene="trpA"
                     /locus_tag="VFU20_03120"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU5285487.1"
                     /translation="MSRYASMFDRLGQRGEGAFGGFLMLGDPDLGTSARLLEALVAGG
                     ADMVEVAIPFSDPVADGPVIQAAAGRALDAGVRTADCFALIAGLRARHPEVPVGILTY
                     ANLVMARGRDDFLRRASEAGADSLLVADLPAREAEPWTDEMRAHGLDPVLIAASNTPP
                     DTLRKIARLGRGYAYCVTRAGITGTHARARFDARLLAELRDAGAPPPVFGFGIATPGD
                     VAAAMAAGAAGVISGSAIVALIADGGEPAPAIERLTARLKAATRADPAD"
     gene            complement(10165..>10989)
                     /gene="trpB"
                     /locus_tag="VFU20_03125"
     CDS             complement(10165..>10989)
                     /gene="trpB"
                     /locus_tag="VFU20_03125"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU5285488.1"
                     /translation="TALAGALLGFETRIYMGAHDAGRQRLNVFRMELMGAEVVAVDSG
                     GRGLKDAINEALRDWSASFPDTHYLLGTAAGPHPFPLLVREFQRIIGREARAQILERA
                     GRLPDAVIACVGGGSNAIGLFADFLGDDVRLIGVEAAGRGLDGGAHGATLLRGRPGIL
                     HGAETYVLQDEDGQIADSWSVSAGLDYPAVGPEHAHLKDSGRAEYVGATDAEALAAFQ
                     SLAENEGILCAFESAHALAHALKLAEREPEALLLVGLSGRGDKDVAQAAQLLGPSQ"
BASE COUNT         1703 a         3788 c         3655 g         1843 t
ORIGIN      
        1 gtcgacaagt ggcaggtgtt cggcggaagc tggggctcga cgctctcgct cgcttacgcg
       61 atcacgcacc ccgagcgggt caccgagctg gtgctgcgcg gcatcttcct gttcggccag
      121 agcgagctcg actggctgta caaatatggc gcgtccgagc tgtttccgga ggggtgggac
      181 gcgttcgttt cgctgatccc cgaggatgag cgcggcgacc tcatcgaggc ctatcgcaag
      241 cggctgaccg gcgacgaccc tgcggagcgc ctggccgcgg ccaaggcgtg gagcacctgg
      301 gaggggctga cggtgactct cctgcccgac ccgaagatgc tcgaggagtt caccgaggac
      361 cagcacgcga tcgccatcgc ccggatcgag aaccattaca tggcaaacaa gggctggctc
      421 gaggacgggc agctgctgcg cggggccgac aagctccgct ccatccccgg ggtgatcgtc
      481 caggggcgcc acgactgctg caccccgcct cgcgccgcct gggaactgaa gcaggcctgg
      541 cccgaggtcg agctgcagat cgtccccgac ggcggccatc tgtacagcga gccgggcgtg
      601 ctcgacgggc tgatccgggc gaccgaccgc ttcgcgtcgc gctagaaatc cggtccgccc
      661 cactgttcgc cgttgactcg gcgggcccga tggacgcagc ggggtaagcg cggcgctcgg
      721 gacacatcgg tccctcgccg cacaggggga aatacgatgc gccgaattct ttgctcgact
      781 tcgctgtacg cgctggccgc cgccgcggcc ctgtccgccg cgcccgccgc cgcccaggcg
      841 accggcgaca ccgacacgcc cgcggccgat gcccagcagg ccgattccca gcagggcgtc
      901 ggggaaatcg tcgtcaccgc gcagcgccgc accgagaacc tgcaggacgt gccgctgtcg
      961 gtcgcgacca tcgccgacga aaccctggcg gcgatcaatt ccggcggcgc cgacgtccgc
     1021 ggcctgtccg gccgggtgcc cagcctaagc atcgagagct cgttcggccg caccttcccg
     1081 cgtttctaca tccgcggcct cggcaacacc gacttcgacc tgaacgcgtc gcagccggtc
     1141 agcctggtct atgacgacgt cgtcctcgaa aacccgatcc tcaaggggtt cccggcgttc
     1201 gacgtcgacc gggtcgaggt gctgcgcggg ccgcagggca cgctgttcgg ccgcaacacg
     1261 ccggccggaa tcgtcaagtt cgacacggtc aagccgggcc gcggccgcaa tttcgcgcgg
     1321 gtcagctacg gcacctacgg cacgatcaat gccgagggcg cggtcggcgg caagctcggc
     1381 gagggcgccg cggcccggct gtcgggcatc tggcagcacc gcaacgactg gatcgacaat
     1441 ctcgacgatc cgggcggcaa cgatctcgag ggctatgacg acttcgcggt ccgcggccag
     1501 gtcgagttcc agccgaccga ggcgctgacg gtgcggctga ccggccagct tcgcgacctc
     1561 gacggcgacg cccgcatctt ccgcgccaac gccatcgagt tcggaagcaa cgatttgatc
     1621 ggcgtcgacg gcggcggctt cgagcgcgac gaggtccgcc acgacgcgat caacttccag
     1681 aaattgaaga cccagaacat cgccggtgcg atcgaatatg atttcggcgc ggcgacgctc
     1741 tattcggtca cgggctactg gcacggcaag ctcgacagcc gcggcgacat cgacggcggc
     1801 ttcggctgct cattctgcgg cccgggcttc accgacaaca gcgagcccgg cttcattccg
     1861 ttcgccgccc agagccagga caatatcccg agcctcgacc agttcaccca ggagatccgg
     1921 atcgcctcca acaaccgcgg cgggctcggc ttccaggcgg gcatcttcta cttcaacgag
     1981 aagctcgaca tcgaaagctt cgatttcgcc acgccgaccg ccccgacccc gtcggcgatc
     2041 gtcaaccaac gccaggacag cgacgccgtc ggcatcttcg ggtcggtcaa ttacgccttc
     2101 gccagcgggc tgaccctgca ggccggagcg cgctggaacc acgacaagaa aaagctcacc
     2161 gccgaccgcc tgttcgacac ccgtttcatc ttcatcggcg gcggcccggt cccggagagc
     2221 acgaccaagg tcaaggacag cgttctgacc tgggacctga gcgcgctcca agagatcaac
     2281 cccgacgtca acgtctatgc ccggatcgcc aggggctatc gggcgccggc aatccagggc
     2341 cggatcctgt tcgaccgcga cgtgacgacg gccgacagcg agcggaccat gtcctacgag
     2401 gccggggtca agacggtgct gctcgaccgc cggctgcggt tcaacctcac cggctaccat
     2461 ttccgcacca aggacctgca gctgtcggcg gtcggcggcg gcaccaacgc caatttgctg
     2521 ctcaatgccg atgcggtcga cggctacggc ttcgaggccg agctgcaggc gcggccggcg
     2581 cgcggcctga ccctgaccgg gggcctgagc tacaaccaca ccaagatcga cgacgacaat
     2641 ctcacggtcg agacctgcgg ggcgccctgc accgtgctcg acccggtcgt cgtcccggcc
     2701 gggctattcc agccggcgat cgtgtcgatc gacggcaacg ccctgccgca ggcgcccagg
     2761 tggatcgcca atttcaccgc cggctacgag catccggtcg gccccggctc ggtctatgtg
     2821 ttcaccgact ggtaccaccg ctcgaagatc cagttcttcc tctaccggtc ggtcgaattc
     2881 tcggacgacc aactgctcga gggcgggttg cgcatcggct acaagaccga gcgcttcgac
     2941 gtcgccggtt tcgtccgcaa catcaccaac gacgaaagcg ccgtctcggg catcgacttc
     3001 aacaatctga cggcgatggt caacgagccg cgcatctggg gggtcgaggt cggggtcaaa
     3061 ttctgaccat tccaacggcg acgcctgccg catcgtcgcc gacggcctag caggtcggca
     3121 tccagtcggt gtggtggatg tcgttgtcgg tcgaaaaggt cgagttgctg ttggtggcgt
     3181 agctgtactt gtaggtcggg cgcttcattc gggcgtaacc gcggtagatg aagcgggtat
     3241 cgttgcaatt gaagtgcgcg agcagcccgg caaccacacg ctcattgctg tagttgccgt
     3301 tcgaccagcg gccaacgcga atctcatcca agccaggccg actgtagctg acgagcaggt
     3361 ccttgctggt ccgagcagtg tcgacgtgct cgatattggc ttcggacagc agcagctgat
     3421 tggccccgtc gagcagcaat tccctgacca catattggga ggtggcctgg tcaaggtcct
     3481 tgtacacgac gccgctgccg tcgtgatacc acgccaggta aaatgcccag ttgacctttc
     3541 ccagctcggt cacggcgccg ttggcggtat cgagcgccaa aatacggttg ttgtcttgct
     3601 cctgcacgaa aatggcaatc ttggacccgt ccagcgagaa gcttagagag tccacgttgc
     3661 ccgggcccgc gtaaagcgtc acgactccgc tcgttcgaac gttgccggtg ccggtcggct
     3721 cataggtcaa aagcttgagg tcgcggtttt cgaggaaggc gacttgcatc tgcgaacgcg
     3781 gacccaggtc gaactgggtg atggccccat atgcgccccg gtgcagggtc acggcgcccg
     3841 tgccgtcctc gtttgccaga cgcaggtcca tgtaattgcc ggaggcgtag gtgtaggcga
     3901 tctcaggcgt aagcggagtt gtcggcttgc ctttctgcgc gggtgcggag gtggccaggg
     3961 tcaacaggac acaagcagct gcaaatcgat tcatgatcgt cccccctaca agacggcgca
     4021 tgcgtctcca ggcagatgcc ccgccgagcc ccggcgactc gccgggcggc cgaccatagc
     4081 agccggtcaa ccgcctggat aacgcacagt taacaaaccg ccggcccgct aggccttggg
     4141 gagcgcctgc ttcgcctggc cccagccggc gagcagcttc gacgaggcgc ggtcgatcaa
     4201 tgtgtccgcg tcgcggatgc tgaacgcgtt gccgccctta tattcgtcga tctcctccca
     4261 ggcgattggc gcggcgaccg gcgcgccttc gcgggcgcgg gccgaataag gcagcaccgc
     4321 ggtcgccccg cgctggtttc tcagccagtc gaggaagatc cggcccttgc gctgggcctt
     4381 gcggatgttg gcggtgaagc gctccggctc ggcctcggag accgcgcgcg agaagcgctc
     4441 ggcgaacgac ttgaccgccg cccagtcggc cttggcgtcg agcgggacga cgacgtgcat
     4501 gcccttgccg ccggtcagca gcgggaagct ggtcagccca aggtcgagga gcaattcgcg
     4561 cagccgcacc gcggcgctcc ggaccgcggc gaaatcgagc ccgacgtcgg gatcgaggtc
     4621 gaacaccagc cggtcgggat gctcgacctt gtcgacccgg ctgccccagc cgtggaactc
     4681 gatagtcccc atctggacgc aggcgagcag gccgcggacg tcgtcgaaat agagataatc
     4741 ttgagtttcg ccctttttct ccttgatcgg gacatgcttg acgtgctcgc ccatcgagcc
     4801 ggtgtcgtgc ttctggaaga agcaatgctt gccgcgcccc tgcgggcagc ggatcagggt
     4861 catcgggcgc cggcctgcgt cgaccatgat cagcgcctcg atctgcgcgt aataatcggc
     4921 gaggtccttc ttggttagcc tgccttcggg atagatcacc cggtcggggt tgctgatcgc
     4981 gattccgaaa tcctcggcgg ccgggcgctt gcccttcttc ttttccgact tggcgagctt
     5041 ttgcgggacc tcgcgaacca cctcggccgc cggcttgtcc tcgcggagcg cgatgaagct
     5101 cgggtggcgc aggatgccgt cgctggtgaa ctcggtgtag gcgatctcgg cgaccagttt
     5161 cggctcgatc caatgggccg ccttgcggtc ggcgcgcggc acctcgagcg gcgccttgtc
     5221 gacctcgagc ggaatcatct tctccatcag atcgtcgatc atccgggtgt tgaagccggt
     5281 tccgaccttg cccgcgtagg tcagcttgcg gccgtcgcgg acgccgagca ggagcgagcg
     5341 gaagccgcgg cgcttgtcgc tttccgacca gccgacgacg acgaactcct ggcgctggat
     5401 gcacttgacc ttgagccagc agcgggtgcg cgtcccgcgg tagggcgcgc tcgccttctt
     5461 cgagatgatc ccctcgccgc cctcggcgca gatggcgttg aacagggcct cgcccttggc
     5521 gacgacatgg tcgccataga tgattggcgg gccgaccccc ttcagcagcg ccgccagccg
     5581 ttccttgcgc tcgagattgg gcaatttgac gatgtcgtcg ccgcggtcga ccagcaggtc
     5641 gaaggcgtag aaagcgaggt tggcgccctt gtgctccttc agggtcgact ggagcagctg
     5701 gaagtcgggc ttgccctggt cgttgagcgc caccgcctcg ccgtcgatca ggcagccggg
     5761 cggaagcttg gccgcggcgc ggaccagcgc ccggaacttg tcgctccagt ccttgccctt
     5821 gcgcgtccag gcggtcgcgg caccgtcgcc ggtcgcgatt agcagccggt agccgtcata
     5881 cttgtactcg tggatccagt ccgacccggc cggcaccgaa tcgaccaggg tcgccagctg
     5941 cggctcgcgg aacggcggcg gcccctgccc cgatttcttc ttcgcccggc cgcccttccg
     6001 cccgccgcgc ttggagcgcc agacgtcgcc gcccgaggcg atctcggcca tcgtccggcc
     6061 ggtggtgacg ctggtcacgc attcgtcgac cagggcgtcg ccggcctcct cgtcggcaaa
     6121 ttcgtcggtg accttcttga gcatccacgg ctcggctttc tcgccggggc gcggcttcag
     6181 gcggaacatc acccactcgc ccttcatccg ctcgccctcg agggtgaaat ggaggtggcc
     6241 atcctcgatc gttttcttgg gatccttgcc gggctcgggg acccagcggc cgcggtccca
     6301 aagcatcacc gtgccgccgc catattcgcc ggccgggatc gtcccttcga agccgccgta
     6361 gtcgagcggg tggtcctcgg tccgcatcgc caggcgcttc tcggcgggat cgaggctcgg
     6421 gcccttgggc accgcccagc ttttcagcac tccgtcgagc tccaggcgga aatcccagtg
     6481 cagccgcgac gcgtcgtgct tctggacgac gaacgagtcg cccttgccct tgagcttgcg
     6541 ccccttcggc tcggcggtct tcgagaagtc gcgcttgcgg ttgtaggtct cgatgtcgag
     6601 tgcggcgcgc ctcgccatcg cctacgcccg cttgcgcgct gcggtcttcc tggccggtgc
     6661 ggcggccttc cgcgccggcg ccttgcgggc cgcgggcttc ctggccgggc cgcgcctgtc
     6721 gccggtcgcg ccgccggttt cgccgaggct cttcttgagc gcggccatca ggtcgatgac
     6781 gttggagccg cgcgacggcg gagcgtcggc gtccgggtcc tggatgacct tctcgccttt
     6841 cttgcgctgc ttctccttga tcagattctt gagcgcatcg acgtagcggt tgtggaactc
     6901 cttggggtcg aagtccccgg tcttcttctc gatcagggtc gcggcgagat cgagcaggtc
     6961 ggggtcgggc ttgtggtcgc cgatctcgcg gaagaagctc gccgcgcggt tgacctcgtc
     7021 ggcgtagcgc agcgtttcga gtagcatgcc gcgcccgcac ggtttcaggg cgacgacata
     7081 ttcctggccg cgcatcgcca gctggccgat gccgaccttg cgcgcctcgc gaagcgcgtc
     7141 gcgaaggacg acgaacgctt cctcggcaag gtcgtcggtg gggacgacgt aatagggctt
     7201 gtcgaaatac atcgggtcga tgtcgcccag gtcgacgaac tggaccaggt cgacggtctt
     7261 gcggctctcg agcttgacgc tctcgatctc ctcgggatcg agcagcacat aatggccctt
     7321 ggagacctca tagcccttca tgatctcgtc gcggtcgacc gggccgattc cgggcaccac
     7381 cttttcgtat ttgatgcgct tgcccgacgg ctcgtgcacc tggtggaact ggaccgtcgc
     7441 gccgcttcgg gtcgccgggt agatctccac cgggatcgac accagcgcca ggcggatctg
     7501 ccctcgccat atcggtcgcg ccgccatctc gcttgcctcc gttcaccagc agccgactca
     7561 atcgctacgg cggggagtcg ttccgtgcgc gtcgatgtgg ggaaacaagc ggcgtccgca
     7621 gccgggcttg cgcggtcgat caggcagcct tcgcgcggtc gcctggaacg ccgctgtggc
     7681 gggccccggt caatcggtag cggaagcccg acgggtcata gctcacttgc gggcggccgc
     7741 cggtttcaag ctggatcccg cgctccagga tccgcattcc gaagcccttg cgcttgggcg
     7801 cctcgacggg cggcccgccg gcctcggtcc attcgaagtc gaagcgggcg tcgttctccg
     7861 ggttcgccct ccaggcgatc gcgatgctcc cgccccgtac cgacagcgcg ccgtacttga
     7921 acgcattggt gctcagctcg tggaccgcca gagacaccat gatcgaggtc gaggaatcga
     7981 tgcacaggtc gggcccgctc agcgatgccc ggtcctcgcc gaccccgcag cccgccagcg
     8041 cctcgcggat gatgtcgctc agcaaggccg agctgccgct ctgggcgtgg aggatgtcgt
     8101 tcgccttggc gatcgagtcg aggcggcggt cgaacgcctt ctgggcctcg gcgacgtcgc
     8161 tggcgctgcc gaagctgagg tgggccatgc cgcgcaccac ggcgaggata ttcttggcgc
     8221 ggtggttgag ctcggcgagc aggagcgcgc gcagctcctc gctccggatg cgctcgtgga
     8281 cccggccgac ctgggcgccg atcgcccggg cggacagcag cagatgctcc tcgggttggc
     8341 gggagtcgcg ggagaagaat tcgagcaccg cgatgcagcg gccgccgctg tgcaccggga
     8401 atccgaacgc cgattcgaaa ccgagcccgg cgcgcatgaa attgtcgtcc gcgttgacgc
     8461 tcgagatcca cagcggcttg ccgctttcga gcactcgtcc cggcaagccg acgcccggct
     8521 cgaagtggat cgccgccgtt gccttgacca gcgccggcgc cgtcttcttc gccttgctgc
     8581 tccaggaccc ctccttgagg gtgcgctggt cctctccgac caggaacgca tggccgaccg
     8641 gccaatcggc gagctcgcag atgccgtcaa ggattgcgac cagcgcttcc tcgaagctct
     8701 tgtcgcggct ggcaaggtcg ctgagctcga gcatcaggcg gctttcgcgc acctggaact
     8761 ccagctgccg ctcggcgcgc ttgcgctcgg aaatgtcgcg caggaaaccg acgaacatgt
     8821 cgcggccgtc gcggctgacc aaggtgatcg acagctcgac cgggacctcg ctgccgtcgc
     8881 ggcgcagccc gctcagctcg agcctcttgt cgagcacctt ggcgatgccc tcggaattga
     8941 accggcgcag gccgcgctgg tgcgcctcgc gcagcgacgg agggacgatc aggtcgccga
     9001 gattctgccc gatcgcttcg ctcgccgacc agccgaaggt ggcctgggca tattggttcc
     9061 aggcgcggat caccccatcg cggtccatga ccacgaccgc atccaggacg gtgtcgagga
     9121 tcggcgccag gaccgacttg atcggcatat gtgcttgaag agcctcctgt ttgctccgaa
     9181 cacccgagcg ttcagccccg taacgagcgc ccctgcggtc ggcaagcttt cggtgtgctg
     9241 aacggctggg cggcgcggcg cgagcgccga tcgggcgccc tcgcccggca ccgaagacgg
     9301 cccgtcggca aggcaatcga cagagccttg ggccggcggg ttggccggcg tccgccccga
     9361 tcggtcaatc ggccgggtcg gcgcgagtcg ccgccttgag ccgggcggtc agtcgttcga
     9421 tggccggtgc gggttcgccg ccgtcggcga tcaacgcgac gatggccgat ccgctgatga
     9481 cgcccgccgc tccggccgcc atcgccgccg cgacatcgcc gggcgtcgca atcccgaagc
     9541 cgaacaccgg cggcggagca ccggcatcgc gaagctcggc gagcaggcga gcgtcgaacc
     9601 gcgccctggc atgagtcccg gtgattccgg cgcgggtcac gcaataggca tagccccggc
     9661 ccagccgagc gatcttgcgc agcgtatcgg gcggcgtgtt ggacgccgcg atcagaaccg
     9721 ggtcgaggcc gtgggcccgc atctcgtccg tccagggctc cgcttcccgg gccggcaaat
     9781 cggcgaccag caagctgtcg gcgcccgcct ccgaggcacg gcggaggaag tcgtcgcgcc
     9841 cgcgcgccat caccaaattg gcgtaggtca ggatcccgac cggaacttcg gggtggcgcg
     9901 cccgcaggcc ggcgatcaga gcgaagcaat cggcggttcg caccccggcg tcgagcgccc
     9961 gcccggccgc cgcctggatg actggcccgt cggcgacggg atccgaaaag gggattgcga
    10021 cctcgaccat gtcggcgccg cccgcgacca gcgcctcgag cagcctggcg ctggtcccga
    10081 gatccgggtc gccgagcatg aggaagcccc cgaaggcgcc ctctccgcgc tgcccgagcc
    10141 ggtcgaacat gctggcgtag cggctcattg cgagggcccg aggagttgcg cggcctgggc
    10201 cacgtccttg tcgccgcgcc cggacaggcc gaccagcagg agcgcttcgg gctctcgttc
    10261 cgcaagcttg agcgcgtgag caagcgcatg cgccgattcg aacgcgcaca ggatgccctc
    10321 gttctcggcc agcgactgga acgcggcgag cgcttctgca tcggttgcgc cgacatattc
    10381 ggctcgcccg ctgtccttga gatgcgcgtg ctcggggccg actgcgggat aatcgaggcc
    10441 ggcggagacc gaccagctgt cggcgatctg cccgtcctcg tcctggagca cataggtctc
    10501 ggcgccatgc aggatgccgg gccgcccgcg cagcagggtc gctccgtgcg cgccgccgtc
    10561 tagcccccgc cccgccgcct cgacgccgat cagccgcacg tcgtcgccga gaaaatcggc
    10621 gaacaggccg atcgcgttcg agccgccgcc gacgcaggcg atcaccgcgt cgggcaggcg
    10681 cccggcccgc tcgagaatct gggcgcgcgc ctcgcggccg atgattcgct ggaactcccg
    10741 caccagcagc gggaaggggt gcggtccagc ggccgtgccg agcagataat gagtgtccgg
    10801 aaagctcgcc gaccagtcgc gcagcgcctc gttgatcgca tccttcaggc cgcggccgcc
    10861 gctgtccacc gccaccacct cggcgcccat cagctccatt cgaaagacgt tgaggcgctg
    10921 ccgcccggcg tcgtgcgcgc ccatgtagat gcgcgtctcg aaaccgagca gggcgccggc
    10981 cagcgcggt
//
LOCUS       DASTZO010000112        10784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_320186,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000112 DASTZO010000000
VERSION     DASTZO010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 10784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10784
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_320186"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFU20_03130"
     CDS             <1..271
                     /locus_tag="VFU20_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5285489.1"
                     /translation="AAAFLVTEIVEFAMLRAPTSPIEVSLCRSSELTATLAIGASVLL
                     PNGDADDIARVQFERILDGLARQLRSPLERKLGRYTVTLPVFHDR"
     gene            complement(397..1236)
                     /gene="tatC"
                     /locus_tag="VFU20_03135"
     CDS             complement(397..1236)
                     /gene="tatC"
                     /locus_tag="VFU20_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU5285490.1"
                     /translation="MKDIDDSKAPLLDHLVELRRRLLWSVAVLAVLFFASLYFATDIF
                     AFLVQPLLAAGEGRIIFTDVFEAFFVEVKVALFTALMLGFPFFAIQLWKFVAPGLYAK
                     EKMALLPFLLLTPVFFIGGAAFAYYLAMPWALHFLLGYQGDVGGVERDALPGIGNYLS
                     FCTRFLFGFGVAFLLPILLMILERAGLVTREQLAKSRRYAIVGAVAIAAVLTPPDAVS
                     QLMLAVPLYALYEFAILAIRITHWRRSRREARSASKVEGPRTNEGPEAPPGGGRVGGS
                     GSI"
     gene            complement(1233..1598)
                     /gene="tatB"
                     /locus_tag="VFU20_03140"
     CDS             complement(1233..1598)
                     /gene="tatB"
                     /locus_tag="VFU20_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HEU5285491.1"
                     /translation="MFGVDSIELIIVAIAALLFIGPKELPAAMRTVGRWIGKMRGMAR
                     HFNAGIESVIREAELEEMDRKWREENERIMREHPIVELGSAAEAQAYLERPEEPLLPL
                     EAPPAPDDDPTPPAERPLP"
     gene            complement(1623..1949)
                     /locus_tag="VFU20_03145"
     CDS             complement(1623..1949)
                     /locus_tag="VFU20_03145"
                     /inference="COORDINATES: protein motif:HMM:NF001940.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU5285492.1"
                     /translation="MGSFSLIHWIILGVVILLLFGGNRFSAMMSDVAKGLKNFKQGLS
                     EDDKPAERAQDQRGEPRSLPPQANREPIDVTPTRTPDPVPPSPPAPTATPAAPPPPAS
                     DEPNRE"
     gene            complement(1980..2546)
                     /gene="scpB"
                     /locus_tag="VFU20_03150"
     CDS             complement(1980..2546)
                     /gene="scpB"
                     /locus_tag="VFU20_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU5285493.1"
                     /translation="MDEFERAVEAALFASANPLSPDEIGEYAGQGDVAAALARLAEHY
                     AGRGIELVERGGRWHFQTAPDLAHLLRRTREEPRRLSRAATETLAIIAYHEPVSRAEI
                     EAIRGVQISKGTLDVLMDAGWVRPAGRREGPGRPLLYATTAEFLSHFGLGSRRDLPGI
                     DDLKAAGLLDPIDEGSFQLQLESEGEED"
     gene            complement(2560..3312)
                     /locus_tag="VFU20_03155"
     CDS             complement(2560..3312)
                     /locus_tag="VFU20_03155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEU5285494.1"
                     /translation="MEAEIFELAVRDEDELTLSIDGWEGPLDLLLTLARAQKVDLAAI
                     SILELVEQYLAYISEARALKLEIAADYLVMAAWLAYLKSCLLLPRDPEAEPSADEIAL
                     RLQLRLQRLDAMREAGARLMGRDRIGRDVFVRGAPEGLRLVRKSAWQARAFDLFAAYG
                     TVKARSMPPLHVVHPRSVMTLEEALERVGRMIGAKLDWTLLEAFLPPTQDPQFRRSAL
                     ASSFLAALELARRGRIDIAQDNAFGPLMVKTA"
     gene            complement(3334..4341)
                     /gene="nagZ"
                     /locus_tag="VFU20_03160"
     CDS             complement(3334..4341)
                     /gene="nagZ"
                     /locus_tag="VFU20_03160"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU5285495.1"
                     /translation="MQAAIYGLAGPELTADERAFFRDADAAGYIVFKRNCIDQAQLLG
                     LTDALRDLSGRPDVPILIDQEGGRVARMRPPHWPSFPAGDAFARLYEAAPSSAIEAAR
                     SNARAIALTLRSCGINVNCLPLLDVRREGAHDIIGDRALGSDPMQVAALGRAVLDGMA
                     SAGVVGVVKHMPGHGRALVDSHKDLPVVTATAEELESDLEPFERLRAAPMGMTAHVVY
                     TAWDPDLPASLSPTVIGDIIRGRIGFEGWLMSDDLGMEALSGEFGARAAGVVAAGCDV
                     ALHCSGDMAEMVAVAGAVPAMSAEGEARLARAMATVLVEDDGADFAEAVAKRDALLAL
                     V"
     gene            complement(4341..5030)
                     /locus_tag="VFU20_03165"
     CDS             complement(4341..5030)
                     /locus_tag="VFU20_03165"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU5285496.1"
                     /translation="MSDSRSGYEDSQLPWLEAVDDEDGPRGVSAKKMLAALLVVLVAA
                     AIVAGTFFWLGRQDPVGIGAPELIRAEPGPYKVKPDDPGGLDVAGESGTAFETSAGQD
                     SDAQLDLNAIAEQPVERPPRDEPKRIPPGETKEPVEDQPRQPATTPPERPSGGSGSVI
                     QLGAYRNAAQAERAWTALSNRFPTISGMTKLVVPYSGGFRLRAGAASPAEARQACQAL
                     RVAGESCFVAN"
     gene            complement(5027..5806)
                     /locus_tag="VFU20_03170"
     CDS             complement(5027..5806)
                     /locus_tag="VFU20_03170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285497.1"
                     /translation="MADGRAAHDPDRLPWLADTRRPRRSSDSTLLLAWAAAATLLVAG
                     VSYWMGMKSVTEPDAFDFAAPSPPAATVRLPEPTAVPPRVQEVLPAPVPDVEPVAEPE
                     PVAIPKPEPVREARAEAGRKAARAKAESAPKARARSAVSKKKAARRPARLQAWPADVS
                     AWAHGRVVRVGTFYTRKQAKQGWSRIVRVYPGMRRLRAVVVPSPSLRNGRTFYRLQFG
                     TTSQAHSAVLCQRMRIVGQSCVVVGLPAGPSAAQARGGVRR"
     gene            complement(5806..7539)
                     /gene="argS"
                     /locus_tag="VFU20_03175"
     CDS             complement(5806..7539)
                     /gene="argS"
                     /locus_tag="VFU20_03175"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU5285498.1"
                     /translation="MSIYAQYSTLLDGVLDELVADGALPAGLERRGVAVEPPRDPAHG
                     DLATNAAMVLAKAAGTNPRALAQLIAPRLEALPPVTSVEVAGPGFINLKLAPDAWRDE
                     LRTILIEGESYGASTIGANERVNVEYVSANPTGPLHMGHCRGAVIADSLARLLEAAGF
                     RVTKEYYVNDAGAQVDALARSVHLRYREALGQDIGAIPEGLYPGDYLAPVGSALAADF
                     GDRYAGAPESEWLDLFRTRAVAAMLELIRHDLALLGVHHDKFASEAELQASGAVDRAL
                     EVLRSKGLVYLGTLERPKSLAPDDEWEPVELTLFRSTQFGDDQDRPLKKADGSWTYFG
                     SDAAYHLLKAESADHLVNIWGADHAGTVKRVQAAVRALTDGRVDLDVKLVQMVRLFRA
                     GEPVKMSKRAGDFVTLAEVVREVGKDVVRFMMLTRRADAPLDFDFAKVVEASKDNPVF
                     YVQYAHARISSLKRKAADAGIDLAAAADLGLLDEEELALVKQAAQYSRVVEAAALAHE
                     PHRIAFFLYELAAQFHALWNRGNDDPGRRFLIEDRKDVSRARLELALGIGQIIKSGLA
                     LMGVEAAEEMR"
     gene            complement(7600..8046)
                     /locus_tag="VFU20_03180"
     CDS             complement(7600..8046)
                     /locus_tag="VFU20_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285499.1"
                     /translation="MENARNLFKLIYKVGMILVTVMLAGGAVLAYVSATPGNGLLLIS
                     GILAVLALIAGLSISKLDDRDRQAETYRQIGQDFRNLVKVSTHRVYSEPEVHRVDMAA
                     LEEARRLDREGASIDSICRLIDPGHDSNDPGHQQAFRKMVRAMLDG"
     gene            8206..8586
                     /locus_tag="VFU20_03185"
     CDS             8206..8586
                     /locus_tag="VFU20_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285500.1"
                     /translation="MSKLSIARVSAAAALLAMSPAVLAQENTANTVDAVDPAANMTAT
                     DPLAANATDVTATDPLANDPMATTTTAADPLAAEPMPNAVDDDDGDDRTFPWGLLGLL
                     GLAGLLGVKRRDDDVRVDTTRDTR"
     gene            8949..9437
                     /locus_tag="VFU20_03190"
     CDS             8949..9437
                     /locus_tag="VFU20_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5285501.1"
                     /translation="MALILIADDDELIVEMVAEALRPRGHIVETLDDGVHVADIVATR
                     HPALVVLDCAMPKMGGIEVLRTIRASATGFDTRVLMLTGRRSGADEEIAIRAGADDYL
                     RKPFAVAELVNRAERLLATETRPGATTTLFNRRRGLERRSDGCSPGDGIAEEVAVRSA
                     SS"
     gene            9451..>10784
                     /locus_tag="VFU20_03195"
     CDS             9451..>10784
                     /locus_tag="VFU20_03195"
                     /inference="COORDINATES: protein motif:HMM:NF020036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU5285502.1"
                     /translation="MPASPRENPDALTAFQLFAVTAATAVAASVLLGWITGTPALTKI
                     LSGSIAMNPMTAVGIMMVSGGVLLRARTHSAKVVWLGGLAALIGIAKLSQLAIGYPVG
                     IDQLMFADQLGAMAGSPPNRMAPNTAFALAMIGLGLAASRSRRGPVIVLSQILCVVAA
                     VTASAALLGYALDEVALYQVHQYIAMALSTALVVLTLSTAIVTTNPNVGLMRIFSDRG
                     PGGSLARIALPLAVLVPLVVSMIGLSGERAGYYGTEAAVAIALIANVLMNFVLLGGCA
                     VVLYRGDIERQRRESAIAQSESHYRQAEKAGHLGYWKLEVPGREVQCSDGFVRICSLP
                     DGTAHDLKTVLSVVHPDDSALVRTCVSQAVELGVDWEFACRIRRPDGSVRYVKSYGVC
                     ECNRHGQTTQVFGVISDVTDLETARRDAEAAKATTASFLANMSHEIRTPMNG"
BASE COUNT         1685 a         3780 c         3635 g         1684 t
ORIGIN      
        1 ggcggcggca ttcctcgtga ccgagatcgt cgagttcgcc atgctccgcg cgccgaccag
       61 cccgatcgaa gtctcgctct gccggtcgag cgaactgacc gcgacactgg cgatcggtgc
      121 gtcggtgctg ctgcccaacg gcgacgcgga cgacattgcg agggttcagt tcgagcggat
      181 tctcgacggc ctggcgcgcc agctacgctc gccactggag cggaaacttg gccgctacac
      241 ggtcaccttg ccggttttcc acgaccgcta atttggttcg gctaagcaac gacattcgtt
      301 gcgaaaattt ttgtgcaccc cgtggaacca gttcggaaaa gtgggcgttt tacagttcgg
      361 atagtgacct tttgcccccc cgctctcgct atccgactaa atggacccgg aaccgccaac
      421 cctccccccc cccggtggcg cttccgggcc ctcatttgtc cggggccctt caaccttcga
      481 ggctgaccgc gcctcgcgcc ggctccggcg ccagtgggtg atccggatgg cgaggatcgc
      541 gaattcgtac agcgcgtaga gcggcaccgc gagcatcagc tgcgacaccg cgtcgggcgg
      601 ggtgagcacc gcggcgatcg ccaccgcgcc gacgatcgca tagcggcgcg acttggccag
      661 ctgctcgcgg gtgaccagcc cggcgcgctc gaggatcatc agcaggatcg gcaacaggaa
      721 cgcgaccccg aagccgaaca ggaagcgggt gcagaagctc agataattgc cgattccggg
      781 cagagcgtcg cgctcgaccc cgccgacgtc cccttggtat cccagcagga agtgcagcgc
      841 ccagggcatc gccagataat aagcgaaggc cgctccgccg atgaagaaca ccggagtcag
      901 cagcaggaac ggcagcagcg ccatcttctc cttcgcgtag aggcccgggg cgacgaactt
      961 ccacaactgg atggcgaaga acgggaagcc gagcatcagc gcggtgaaca gggcgacctt
     1021 cacctcgacg aaaaaggcct cgaacacgtc ggtgaagatg atccggccct cgccggcggc
     1081 gagcagcggc tggaccagga aggcgaagat gtccgtcgcg aaatagaggc tcgcgaagaa
     1141 cagaacggcg agcaccgcga ccgaccacaa cagccgccgg cgcagctcga cgaggtggtc
     1201 gagcaacggc gccttgctgt cgtcgatgtc cttcacggca gcggccgctc ggccggcggg
     1261 gtcgggtcgt cgtcgggcgc cggcggcgcc tcgagcggca gcaacggctc ttcgggccgt
     1321 tcgagatagg cctgggcttc cgcagccgaa cccagctcga cgatcgggtg ctcgcgcatg
     1381 atgcgctcat tctcctcgcg ccacttgcgg tccatctcct cgagctcggc ctcgcggatg
     1441 accgattcga tgccggcgtt gaagtggcga gccatgcccc gcatcttgcc gatccagcgg
     1501 ccgacggtgc gcatcgccgc cggcagctcc ttggggccga tgaacaagag cgccgcgatc
     1561 gcgacgatga tcagttcgat ggaatcgacg ccgaacatca gccggccgtc ccgggtccgg
     1621 ggctattcgc ggttcggctc gtcgctggcc gggggcgggg gcgccgccgg cgtggcggtc
     1681 ggggccggcg gcgagggcgg caccgggtcg ggcgtgcgcg tcggggtgac gtcgatcggc
     1741 tcgcggttgg cctgcggcgg aagcgagcgc ggctcgccgc gctggtcctg ggcacgctcg
     1801 gccggcttgt cgtcctccga caacccctgc ttgaagttct tcaggccctt ggcgacgtcg
     1861 ctcatcatcg ccgagaagcg gttgccgccg aacagcagca ggatgacgac gccgaggatg
     1921 atccagtgga tcaggctgaa actgcccata tgctcactcc gaaaaggccc ggcgacctct
     1981 tagtcttcct cgccttcgct ttccagttgc agttgaaacg acccttcgtc gatcgggtcg
     2041 agcaggccgg cggccttgag gtcgtcgatc cccggcaggt cgcgcctcga gcccaggccg
     2101 aaatgcgaca ggaactcggc ggtggtcgcg tagagcaagg gccggcccgg accctcgcgg
     2161 cggccggcgg gccgcaccca gccggcgtcc atcagcacgt cgagcgtgcc cttggagatc
     2221 tggactccgc ggatcgcctc gatctcggcc cggctgaccg gctcgtgata ggcgatgatc
     2281 gccagcgttt cggtcgccgc gcgcgacagc cgccgcggct cctcgcgggt ccggcggagc
     2341 aaatgcgcga ggtcgggcgc ggtctggaaa tgccagcggc cgccccgctc gaccagctcg
     2401 atcccgcgcc cggcgtaatg ctcggcgagc cgggcgagcg ccgcggcgac gtcgccctgg
     2461 ccggcatatt cgccgatttc gtcggggctg agcgggttgg cggacgcgaa cagcgccgct
     2521 tcgaccgccc gttcgaactc atccatcagc cctggtccat caggcggtct tcaccatcag
     2581 cggcccgaac gcattgtcct gggcgatgtc gatccgcccg cggcgcgcga gctccagcgc
     2641 ggcaaggaag ctcgaggcca gcgccgagcg gcggaactgc gggtcctggg tcggcggcag
     2701 gaacgcctcg agcagggtcc agtcgagctt ggcgccgatc atccgcccga cccgctcgag
     2761 cgcttcctcc agcgtcatca ccgatcgcgg atggacgacg tgcagcggtg gcatcgagcg
     2821 cgccttgacc gtgccgtaag ccgcgaacag gtcgaaggca cgcgcctgcc acgccgactt
     2881 gcgcaccagc ctcagcccct cgggggcgcc gcggacgaac acgtcgcggc cgatccggtc
     2941 gcggcccatc agccgcgccc cggcctcgcg catcgcgtcg aggcgctgca gccgcagctg
     3001 cagccgaagc gcgatctcgt cggcgctcgg ctcggcctcg gggtccctgg gcagcagcag
     3061 gcaggatttg aggtaggcga gccacgcggc catcacgagg taatcggcgg cgatctcgag
     3121 cttcagcgcc cgcgcttcgg agatgtaggc gagatattgc tcgaccagct cgaggatcga
     3181 gatcgcggcg aggtcgacct tctgcgcgcg cgccagggtc aggagcaggt cgagcggccc
     3241 ttcccacccg tcgatgctga gcgtcagctc gtcctcgtcg cgaaccgcca gctcgaaaat
     3301 ctcggcttcc atcgcgcgag cctatcgcct cgctcaaacc agcgccagca gcgcgtccct
     3361 tttggccacc gcttcggcga agtcggcgcc atcatcctcg accagcacgg tcgccatcgc
     3421 ccgcgccagc cgagcctcgc cttcggcgct catcgccggc accgcgccgg cgaccgcgac
     3481 catctcggcc atgtcgcccg agcaatgcag agcgacgtcg cacccggccg cgacgacgcc
     3541 cgccgcgcgg gccccgaact cgccggacag cgcctccatc ccgaggtcgt cgctcatcag
     3601 ccagccctcg aagccgatcc ggccgcggat gatgtcgccg atcaccgtcg gcgacaggct
     3661 cgccggcaaa tccgggtccc aggcggtgta gacgacgtgc gcggtcatgc ccatcggcgc
     3721 cgcgcgcagc cgctcgaacg gctcgaggtc gctttccagc tcctcggcgg tcgcggtgac
     3781 gacgggcagg tccttgtggc tgtcgaccag cgcccggccg tggcccggca tgtgcttgac
     3841 caccccgacc actccggccg acgccattcc gtccagcacc gcccggccga gcgcggcgac
     3901 ctgcatcggg tccgagccca gcgcccggtc gccgatgata tcgtgcgcgc cctcgcggcg
     3961 gacgtcgagc agcggcaggc agttgacgtt gattccgcac gagcggagcg tcagcgcgat
     4021 cgcccgggcg ttcgagcgcg ccgcctcgat cgccgacgac ggcgccgcct cgtacagccg
     4081 ggcgaacgca tcgccggcgg gaaacgacgg ccagtgcggc ggccgcatgc gggccacccg
     4141 cccgccctcc tggtcgatca ggatcggcac gtccgggcgg ccggacaggt cgcgcagcgc
     4201 gtcggtcagc ccaagcagct gcgcctggtc gatgcaattg cgcttgaaga cgatgtagcc
     4261 ggcggcgtcg gcgtcgcgga agaacgcacg ctcgtccgca gtcagctccg gtcccgcgag
     4321 cccgtagatc gccgcctgca tcaattggcc acgaagcagc tttcgccggc gacccgaagc
     4381 gcctggcacg cctgccgggc ttcggccggc gaggccgccc ccgcgcgcag ccggaagccg
     4441 cccgaatagg gcaccaccag cttggtcatc cccgagatgg tcgggaagcg gttcgacagc
     4501 gccgtccacg cgcgctcggc ctgcgcggcg ttccggtagg cgccgagctg gatcacgctg
     4561 cccgaaccgc cgctcggccg ctcgggcggc gtggttgcgg gctgccgggg ctggtcctcg
     4621 accggctcct tggtctcgcc cggcgggatc cgcttcggct cgtccctcgg cgggcgctcc
     4681 accggctgct cggcaatcgc gttgagatcg agctgggcgt cgctgtcctg gccggcgctg
     4741 gtctcgaacg cggtgccgct ctcgccggcg acgtcgagcc cgccggggtc gtcgggcttg
     4801 accttgtagg gcccgggctc ggcgcggatc agctccgggg cgccgatccc gaccgggtcc
     4861 tggcggccga gccagaagaa ggtgccggcg acgatcgcgg cggcgaccag caccaccagc
     4921 aaggcggcga gcatcttctt cgccgagacc ccgcgcggcc cgtcctcgtc gtcgaccgct
     4981 tccagccacg gcagctggct gtcctcatag cccgaacggc tgtcgctcat cgccgaaccc
     5041 ctccccttgc ctgcgccgcg gatgggcccg ccggcaagcc gaccaccacg caactctgcc
     5101 cgacgatccg catgcgctgg cacaggacgg cggaatgcgc ctgcgacgtg gtcccgaact
     5161 ggagccggta aaacgtccgg ccgttgcgca gcgacgggct cggcacgacc accgcgcgca
     5221 gccgccgcat gccgggatag acccgcacga tccgcgacca gccctgcttc gcctgcttgc
     5281 gggtgtagaa ggtgccgacc cgcaccaccc gcccgtgcgc ccaggccgac acgtccgccg
     5341 gccacgcctg aagccgcgcc gggcgcctgg ccgccttttt cttcgacacc gccgatcggg
     5401 cgcgagcctt tggcgccgat tcggccttcg ccctggccgc cttgcgcccc gcctcggccc
     5461 gggcctcgcg caccggttcg ggcttgggga tggcgaccgg ctcgggctcg gcgaccggct
     5521 cgacgtcggg gaccggcgcc ggcagcacct cctgcacacg cggcggcacg gcggtcggct
     5581 cgggcagccg caccgtcgcc gccggcggcg agggcgcggc aaagtcgaag gcatccggct
     5641 cggtgacgct tttcatgccc atccagtagg aaaccccggc gaccagcagg gtggcggcag
     5701 cggcccaggc cagcaacagc gtcgagtcgc tcgagcggcg tggcctccgg gtgtcggcga
     5761 gccaaggcag ccggtccggg tcgtgggccg cgcggccgtc ggccatcagc gcatctcctc
     5821 cgccgcctcg actcccatga gcgcgaggcc gctcttgatg atttgcccga tgccgagcgc
     5881 caattccagc cgcgcccgcg aaacatcttt tcggtcctcg atcaggaaac gccgtcccgg
     5941 atcgtcgttg ccgcggttcc acagagcgtg gaactgggcc gccagctcgt agaggaaaaa
     6001 ggcgatccgg tgcggctcgt gggccagcgc ggcggcctcg accacgcgcg aatattgcgc
     6061 cgcctgcttg accagcgcca gctcctcctc gtcgagcagg ccgaggtccg ccgccgcggc
     6121 caggtcgatg cccgcgtccg cggccttgcg cttcagcgaa ctaatccgcg cgtgcgcata
     6181 ttggacgtag aacaccggat tgtccttcga cgcctcgacc accttggcga agtcgaagtc
     6241 gagcggtgcg tcggcgcggc gggtcagcat catgaaccgg acgacgtcct tgccgacctc
     6301 gcgaacgacc tcggccaaag tgacgaaatc gccggcgcgc ttcgacatct tgaccggctc
     6361 gccggcgcgg aacagccgca ccatctggac cagcttgacg tcgagatcga cgcggccgtc
     6421 ggtcagcgcc cggaccgccg cctggacgcg cttgacggtg ccggcgtggt cggcgcccca
     6481 gatgttgacc agatggtcgg cgctttcagc cttgagcaaa tgataggcgg cgtcggagcc
     6541 gaaataggtc cagcttccgt cggctttctt caatggccgg tcctggtcgt cgccgaattg
     6601 ggtcgagcgg aacagggtca gctcgacggg ctcccattcg tcgtccggag caaggctctt
     6661 gggccgctcg agcgtgccca ggtagaccag gcccttggag cgcagcacct cgagcgctcg
     6721 atcgaccgcg ccggaggcct ggagctcggc ctcggacgcg aacttgtcgt ggtggacgcc
     6781 gagcagcgcg aggtcgtggc gaatgagctc gagcattgcc gcgaccgcgc gggtccggaa
     6841 caggtcgagc cactcgcttt ccggcgcgcc ggcatagcgg tccccaaaat ccgccgcgag
     6901 ggcgctgccg accggcgcca gataatcgcc cgggtagagg ccttcgggga ttgcgccgat
     6961 gtcttgcccg agcgcctcgc ggtagcgcag gtgcaccgag cgggcgagcg catcgacctg
     7021 cgccccggcg tcgttgacat aatattcctt ggtcacccgg aagccggccg cctcgagcag
     7081 ccgcgcgagg ctgtccgcga tcaccgcgcc gcggcaatgg cccatgtgca gcggcccggt
     7141 cgggttggcc gacacatatt cgacgttgac ccgctcgttc gcgccgatcg tcgaggcgcc
     7201 gtaggactcg ccctcgatca ggatcgtcct gagctcgtcg cgccacgcgt cgggcgccag
     7261 cttcaaattg atgaacccgg gcccggcgac ctcgaccgac gtcaccggcg gaagcgcctc
     7321 gagcctgggc gcgatcagct gggcgagcgc gcgcgggttg gtgccggccg ccttggccag
     7381 caccatcgcc gcattggtcg caaggtcgcc gtgcgccggg tcccgcggcg gctcgaccgc
     7441 gaccccgcgc cgctcgagcc cggccggcag ggcgccgtcg gcgacaagct cgtcgagcac
     7501 cccgtcaagg agtgtggaat attgggcgta gatggacatc ggctgcgcgt cctagcgcag
     7561 gaagtccggg cggtcacccg aactcgcggg gctttccgat caaccgtcga gcattgcccg
     7621 gaccattttc cggaacgcct gctggtggcc agggtcgttc gagtcatggc cggggtcgat
     7681 cagccggcaa atcgagtcga tcgacgcgcc ctcccgatcc agccggcggg cctcctcgag
     7741 cgccgccatg tcgactcgat gaacttcggg ctcggaatag acgcggtggg tggaaacctt
     7801 gaccaggttg cggaaatcct ggccgatctg gcggtaggtt tcggcctgcc ggtcgcgatc
     7861 gtcgagcttg ctgatcgaca gcccggcgat caacgccagc acggcgagaa tcccgctgat
     7921 caggagcagc ccgttgccgg gagtcgcgct gacgtaggca agcacggcgc cgccggcgag
     7981 catcaccgtg acgaggatca tgccgacctt gtagatcagc ttgaacaggt ttctcgcatt
     8041 ctccatccca gcgcccccgc cgatgacttt tcgaacggcg cgaactatgg cccgatcgtc
     8101 gaattttgcc aatcggcgcc caatcggcgc cgggtcggga acggggaacg gctttggtcc
     8161 gttggccggc tgcggcatca ccagagccgt caacaggagg aagcaatgtc gaagctttcc
     8221 attgcccggg tgtccgcggc cgcagccttg ctcgcgatga gccccgccgt ccttgcccag
     8281 gaaaataccg ccaataccgt cgatgccgtc gatccggcgg cgaacatgac ggcgaccgac
     8341 ccgctggcgg ccaacgcgac cgacgtgacg gcgaccgatc cgctggccaa cgacccgatg
     8401 gcgacgacga ccaccgccgc cgacccgctg gcggccgagc cgatgccgaa cgcggtcgac
     8461 gatgacgacg gcgacgaccg gacgtttccc tggggcctgc tcgggctgct cgggctggcc
     8521 ggactgctgg gggtgaagcg ccgcgacgac gacgtgcgcg tcgacacgac ccgcgatacc
     8581 cgctgatcga ctgaagccgc gcgccctgtc cctagcatgc gtgcgcgccc ggcgccgcgg
     8641 ccgcaacgcc gcggcgcatc ggcgtcaggg ctgggaagaa atcccatgtg agggatcagg
     8701 gctactcatc cgcttcggat gtaagcgagg ctcgccccct tgcccttgcg aagacgatgt
     8761 tctgtaaaag ggcagtcttt ctgcagatag atgagactag cctgttttct tggggtgcca
     8821 aatactaccc atactggatc aatgattgat ccataatgct ttgtattaat ttactttgca
     8881 tgcactatta atatttatcg ggcctgactt ctttcctact tgctcgcatt gctcgaggga
     8941 gacgattgat ggccctgatc ttgattgccg acgacgacga gctgatcgtc gaaatggtcg
     9001 ccgaggcgct gcgcccacgc ggccacatcg tcgaaacgct cgacgacggg gtccacgtcg
     9061 ccgacatcgt cgccaccagg cacccggcgc tggtggtcct cgactgcgcg atgccgaaga
     9121 tgggcgggat cgaggtgctg cggacaatcc gtgcgtcggc gaccggcttc gatacccggg
     9181 tgctgatgct gaccggccgg cgaagcggcg ccgatgagga aattgccatt cgagccggcg
     9241 ctgacgatta tctgcgcaag cccttcgccg tggccgagct ggtcaaccgg gccgaaaggc
     9301 ttcttgcaac cgaaacgcga ccgggcgcga cgacgacctt gttcaatcgc cgcaggggcc
     9361 tcgagcggag gagcgacggg tgcagtccgg gcgacggcat tgcggaagag gtcgccgtcc
     9421 ggtctgcctc gtcctgaccg ggtcgagatc atgccggcaa gcccgcggga aaatcccgat
     9481 gccctgaccg ccttccagct gtttgcggtc accgccgcga ccgcggtcgc ggcgagcgtg
     9541 ctgctcggat ggatcaccgg cactcccgca ctgacgaaaa tcctgtccgg ctcgattgcg
     9601 atgaatccga tgacggccgt cggcatcatg atggtttcgg gcggggttct tctgcgcgcc
     9661 cgaacccatt cggcgaaggt tgtctggctc ggcggattgg cggcgctcat cggcatcgcc
     9721 aagctgtccc agctggcgat cggctatccg gtcggcatcg accagctgat gttcgccgac
     9781 cagctcggcg caatggccgg ctcacctccc aaccggatgg cgccgaatac ggccttcgcc
     9841 ctggcgatga tcggcctcgg cctcgcggca agccgttcgc gtcgcggccc ggtcatcgtg
     9901 ctgtcccaga ttctttgcgt ggtggcggcg gtcaccgcct cggcggcgct gctcggatat
     9961 gccctcgacg aggtcgcact ctaccaggtc caccaatata tcgcgatggc gctgtcgacc
    10021 gccctggtcg ttctcacatt gtcgaccgcc atcgtcacga ccaaccccaa tgtcggcctg
    10081 atgcggattt tcagcgaccg cggccctggg ggctcgctcg cccggatcgc gctgccgctc
    10141 gccgtgctgg ttccgctggt cgtcagcatg atcggcctgt cgggggaacg cgccggctat
    10201 tacgggaccg aggcagcggt ggcgatcgcg ctgatcgcga acgtgctgat gaacttcgtg
    10261 ctgctcggcg gctgcgcggt cgtgctctac cgcggcgaca tcgagcgcca gcggcgcgaa
    10321 tccgcgatcg cgcaaagtga atcccactat cggcaggccg aaaaggccgg gcacctcggc
    10381 tattggaagc tggaagtgcc cggacgggaa gtgcaatgtt ccgacgggtt cgttcgcatc
    10441 tgctctctgc cggacgggac cgcccacgac ctgaaaacgg tcctgtccgt cgttcatccc
    10501 gatgactcgg cactggtgcg gacgtgcgtg tcgcaagccg ttgagctggg cgtcgactgg
    10561 gaattcgcgt gccgcatccg ccgaccggac ggttcggtcc gctacgtcaa gtcgtacggc
    10621 gtgtgcgaat gcaaccggca cggccagacg acccaggtgt tcggggtgat ttccgacgtc
    10681 accgacctgg agacggcgcg ccgcgacgcc gaggcggcga aggcgacaac cgcatccttc
    10741 ctcgccaaca tgagccacga aatccggact ccgatgaacg gcat
//
LOCUS       DASTZO010000113         2373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_687739,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000113 DASTZO010000000
VERSION     DASTZO010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2373
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687739"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="VFU20_03200"
     CDS             complement(<1..886)
                     /locus_tag="VFU20_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5285503.1"
                     /translation="MMQPPTIDDIRSAARRIEGAVIHTPMMISRTLSEIIGAEVWLKF
                     ENLQFTGAYKERGALNKLLQLTDEERARGVIAASAGNHAQAVAYHGRRLGIPVTIVMP
                     APTPTIKVSQTAGHGARVILEGAMFDDAHAKACELAAQDGYVFVHPFDDPQVIAGQGT
                     VALEMLAAAPDLDMLVVPVGGGGLMSGVGIAARALKPGVGLIGVEAELYPSMKCAIEG
                     CELPLGGDTLAEGIAVKQPGELTARIIGEQVNDVVLVAERDIERAVAMLVGIEKTVVE
                     GAGAAGLAARLADPARFKG"
     gene            942..>2373
                     /locus_tag="VFU20_03205"
     CDS             942..>2373
                     /locus_tag="VFU20_03205"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5285504.1"
                     /translation="MLKVLPALIAALLAAPANADTLIDNANGIQVRADGGLERFDALL
                     IGDDGKVVRTLAKGEPRPRADARVDAGGRALLPGLIDAHGHVIDAPGGDMGLGLMLLQ
                     LDLNGTGSIAELKRRTAAYAAANPDADWILGRGWNQELWAGKRLPTAADLDSVVADRP
                     VLLARVDGHAAVANSAALKAAGITAATPDPQGGTIDRHPDGSPTGLLIDNAILLVARR
                     APPITSEMMSRALLSAQAFLLSNGITAVADMGTSASGWDTMRRLGQTGALKMRIISYF
                     DGLEPGQRTPTKGPTPWLHGDRLRMVGVKLYADGALGSRGAWLKSPYADKPGTRGLQF
                     LTDAQLREQAGKAAAAGFQVAVHAIGDAANAQVIGAFEELRKTHTGDRRWRIEHVQVV
                     DPADIPRIGRAGIIASMQPVHQTSDRTMAEARLDPPRLAGAYAWNSIARTGARLAFGS
                     DFPVESPNPFPGLAAAVSRQDPNGIPP"
BASE COUNT          346 a          884 c          805 g          338 t
ORIGIN      
        1 gccccttgaa gcgcgccggg tcggcgagcc tcgccgccag ccccgccgcg cccgccccct
       61 cgacgaccgt cttctcgatt ccgacgagca tcgccaccgc ccgctcgatg tcgcgctcgg
      121 cgacgaggac gacgtcattg acctgctcgc cgatgatccg cgccgtcagc tcgcccggct
      181 gcttgaccgc gatcccttcc gcgagcgtgt cgccgccgag cggcagctcg cagccctcga
      241 tcgcgcattt catcgacggg tagagctcgg cctcgacccc gatcaggccg acgccgggct
      301 tcaaggcccg cgccgcgatc ccgactcccg acatcagccc gccgccgccg accgggacca
      361 ccagcatgtc gaggtcgggc gcggccgcga gcatctcgag cgcgaccgtg ccctgcccgg
      421 cgatcacctg cgggtcgtcg aacgggtgga cgaacacata gccgtcctga gcggcgagct
      481 cgcacgcctt ggcgtgggcg tcgtcgaaca tcgccccctc gaggatcacc cgcgctccgt
      541 gccccgccgt ctggctgacc ttgatggtcg gggtcggcgc cggcatgacg atggtcaccg
      601 ggatgccgag ccgccggccg tggtaggcga ccgcctgcgc atggttgccg gccgacgccg
      661 cgatcacccc gcgggcgcgc tcctcgtcgg tcagctggag caatttgttg agcgcgcccc
      721 gctccttgta ggcgccggtg aactggaggt tctcgaactt cagccagacc tcggcgccga
      781 tgatctcgga cagggtccgg ctgatcatca tcggcgtgtg gatgaccgcg ccctcgatgc
      841 gccgggccgc ggagcggatg tcgtcgatcg tcggcggctg catcatcggt ggcgaggcct
      901 agcgcttcac ccactggcaa acaatgctat gccgccgggc catgctgaag gtccttcccg
      961 cgctgatcgc cgcgctgctc gccgcgcccg cgaacgccga cactctgatc gacaacgcca
     1021 acggcatcca ggtccgcgcc gacggcgggc tggagcgctt cgacgccctg ctgatcggcg
     1081 acgacggcaa ggtcgttcga acgcttgcca aaggcgagcc gcggccccgc gccgatgccc
     1141 gggtcgacgc cgggggccgg gccctgcttc ccggactgat cgatgcgcat ggccatgtga
     1201 tcgatgctcc gggcggcgac atggggctgg gcctcatgct cctgcaactg gatctcaacg
     1261 gcaccggctc gatcgccgag ctcaagcgcc ggaccgcagc ctacgccgcg gcgaacccgg
     1321 atgccgactg gattcttggc cgcggctgga atcaggagct ttgggccggc aagcgtctcc
     1381 cgactgcggc cgacctggac tcggtggtcg cggaccggcc ggtgctgctg gctcgcgtcg
     1441 acggtcacgc cgccgttgca aactcggcgg cactgaaagc tgccgggatc accgccgcca
     1501 cccccgaccc gcagggcgga acgatcgatc gccatccgga cggctcgccc accggcctgc
     1561 tgatcgacaa tgcgatcctt ctggttgctc gccgggcgcc gccgatcact tcggaaatga
     1621 tgagccgcgc gctgctgtcc gctcaagcgt tcctgctgag caacggcatc accgctgttg
     1681 ccgatatggg aacctcggcg agcggctggg acacgatgcg gcggctcggg caaaccggcg
     1741 cgctgaagat gcgcatcatc agctacttcg acgggctcga gccagggcag cggacgccga
     1801 cgaagggccc gaccccgtgg ctccacgggg accggctgcg gatggtcggg gtcaagctct
     1861 acgccgacgg ggccttgggc tcgcgcggcg cgtggctcaa gagcccgtac gccgacaagc
     1921 cgggcacgcg cggcctgcaa ttcctgaccg acgcgcagct gcgcgagcag gcgggcaagg
     1981 cggccgcggc cggattccag gtcgcggtcc acgcgatcgg cgacgccgcc aacgcgcagg
     2041 tgatcggcgc cttcgaagag cttcggaaaa cccataccgg cgaccggcgc tggcggatcg
     2101 agcatgtcca ggtcgtcgac ccggccgaca tcccgcgcat cggccgcgcc gggatcatcg
     2161 cctcgatgca gcccgtccac cagaccagcg accggacgat ggccgaggcg aggctggacc
     2221 cgccgcggct ggccggcgcc tatgcgtgga acagcatcgc gcggaccggc gcccggctcg
     2281 cgttcggctc cgacttcccg gtcgaatcgc ccaatccgtt ccccggcctc gccgcggcgg
     2341 tcagccggca ggacccgaac ggaatcccgc ccg
//
LOCUS       DASTZO010000114         6975 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1158159,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000114 DASTZO010000000
VERSION     DASTZO010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6975
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158159"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VFU20_03210"
     CDS             complement(<1..447)
                     /locus_tag="VFU20_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5285505.1"
                     /translation="MNWRGIWAIYKFEMHRFGRTLWTGLAVPVITTSLYFIVCGAAIG
                     SRMSEIGGIDYGSFIVPGLMMLSLFTESIFNASFGIHMPRFTGTIYEILSAPLSAFET
                     VLGYVGAAATKAMTVALVILATANLFVDVRIEHPLFMLGFLLLVAVT"
     gene            complement(444..1397)
                     /locus_tag="VFU20_03215"
     CDS             complement(444..1397)
                     /locus_tag="VFU20_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5285506.1"
                     /translation="MSGTILSIRGLRKSYASGTEALKSVDLDIERGEIFALLGPNGAG
                     KTTLINIVCGTVTPSGGTVLVDGKHWLRNYREARTRIGLVPQELTMDVFEPLISTVSF
                     SRELFGRAPDRAKVERILRDLSLWDKRKQILKELSGGMKRRVMIAKALAHEPDILFLD
                     EPTAGVDVELRRDMWGLVRRLREDGTTIILTTHYIEEAEEMADRVGVINKGELILVEE
                     KHALMEKLGRKVLHLQLAEPLAAVPPELADWQPQLSDEGNTLTYEFDARAERTGIPSL
                     LSAMRDAGIAFKDLDTKQSSLEDIFVGLIHEQRDEHRSAAQ"
     gene            complement(1394..2008)
                     /locus_tag="VFU20_03220"
     CDS             complement(1394..2008)
                     /locus_tag="VFU20_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HEU5285507.1"
                     /translation="MSDPREPSGGTRLLIDLGPLLIFFLTNYLAPVPDVLRIFVATGA
                     FMAAMIAAMIYSQLRFRRISPLLLFSGFMVIVLGGLTIWLRNETFIKIKPTIYYLLVS
                     GLLGFGLATGRPLLKAVLGSTYPGLDSAGWTKLTRNWALFFAGMAMLNEAVWRNSSTD
                     FWVGFKLWGAIPLTFLFAAANVPMLLRHGLARDEAQPAEPGPVE"
     gene            complement(2020..2934)
                     /gene="ftsY"
                     /locus_tag="VFU20_03225"
     CDS             complement(2020..2934)
                     /gene="ftsY"
                     /locus_tag="VFU20_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU5285508.1"
                     /translation="MSWLDRLRGGFTKTAARLGENLTGLTTTAALDTATLDDIEEALI
                     ASDLGPEASRRIREAIATRRFEQLDERGLRTILAEEIEAILAPVAKPLEVWGFPRPHV
                     VLVIGVNGSGKTTTIGKLGHWLQEQDYGVLLAAGDTFRAAAIEQLQIWGQRIGAPVIA
                     GKEGGDAAGIVFDGVRQATETGIDVLVVDTAGRLQNKTHLMDELSKIRRVLGRINPEA
                     PHDVLLVLDATTGQNALAQVEVFRDSAGVTGLIMTKLDGTARGGILVAAAEKFGLPIH
                     AIGVGEGVDDLRPFDPRAVARAIAGLPQ"
     gene            complement(2931..4073)
                     /locus_tag="VFU20_03230"
     CDS             complement(2931..4073)
                     /locus_tag="VFU20_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5285509.1"
                     /translation="MSVETITLGCRLNFAESEAMRGLVPGKDDWVIVNSCAVTAEAVR
                     QSRQAIRRARRERPGAKILATGCAVQLEPDRFRAMPEVDEIRMRDFAAPPAVPAGAHA
                     RVRSFVGIQTGCDHRCTFCSIWQARGPSRSFPFEAIRDAVAREIDRGAKEIVLTGVDI
                     TDYRGGLGSLCRRLLAAEPRLERLRLSSLDSVEIDDELMDLIAGEPRLMPHFHLSLQA
                     GDDLILKRMKRRHSRADAVRTVERIKASRPDATIGADLIAGFPTETEDMALNSLKLLD
                     DCDVIAAHVFPFSPRPGTPAARMPQVDRETVKARAARLREAAAARRSAWLDSLIGTTQ
                     RVLVESPGKGHSDGFAPVRIEGAERGEIRDARITGRDGDHLVGCCA"
     gene            complement(4070..4900)
                     /gene="dapF"
                     /locus_tag="VFU20_03235"
     CDS             complement(4070..4900)
                     /gene="dapF"
                     /locus_tag="VFU20_03235"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEU5285510.1"
                     /translation="MAHGFHKMPFHKMEGLGNDFAIFDAREEPLAMSEALARALADRH
                     KGIGCDQLIVLEPSDTADVRMRIWNHDGGEVESCGNAARCVVALTGATSIETDGGLVS
                     GAADGSEVEVAIGEPRFGWDEVPLAYAMDTAALPMAWGELEMPTALSVGNPHLVFFVE
                     DAEAIPLEGLGPLIEHDPAFPDRINVSVAQVGGGGIRLRTWERGAGLTLACGTGACAA
                     AVAAIASRRAQSPVKVTMPGGSLTIAWQPGEPVRMRGPATYVFRGEIDLEKLTGGSKG
                     "
     gene            5096..6571
                     /gene="ffh"
                     /locus_tag="VFU20_03240"
     CDS             5096..6571
                     /gene="ffh"
                     /locus_tag="VFU20_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU5285511.1"
                     /translation="MFDSLSDRLSSVFDKLRGRGALSEADVRSAMREVRVALLEADVA
                     LPVARDFVDQVTERAVGQEVLRSVTPGQMVVKIVHDALVGMLGSEASELRLGVNPPAV
                     ILMVGLQGSGKTTTTAKLGKRLAERERKKVLMASLDVARPAAQAQLAVLGRQANVDTL
                     PVVAGQQPVDIAKRAIQAARLQAYDVVLLDTAGRLHVDDALMAEMKAVAAASSPTETL
                     LVVDALTGQDAVNVARGFAGEVDLTGVILTRLDGDARGGAALSMRAVTGKPIKFAGVG
                     EAIDKLEAFHPDRVAGRILGMGDVVSLVERAAETIEVEEAEKLAAKMVKGKFDLDDLR
                     AQIQQMQRMGGLGALASMMPGLKTVKGAAEKAADGKALVHLEAMMSSMTAKERSRPEL
                     INAKRKIRIAKGSGTTVQEVNKLLKMHQEMASVMKRLKKMGGLGKLAAMFGKGGLEGA
                     LGGVAPGMLPPGALGGGGPALPGLGGSPFNLPPGFDKFTKK"
     gene            6601..>6975
                     /gene="rpsP"
                     /locus_tag="VFU20_03245"
     CDS             6601..>6975
                     /gene="rpsP"
                     /locus_tag="VFU20_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU5285512.1"
                     /translation="MALSIRLTRGGAKKRPYYRIVVADSRAPRDGRFIEKLGSYNPLL
                     AKDSPERVKLDAERIAHWLSVGAQPSDRVLRFLDAAGIRERPARNNPKKGEPGEKAKA
                     RIEERAGKAAAAAEAAEAAAAAP"
BASE COUNT         1087 a         2419 c         2418 g         1051 t
ORIGIN      
        1 ggtcaccgcg accagcagca ggaagccgag catgaacagc gggtgctcga tgcggacgtc
       61 gacgaacagg ttcgcggtcg cgaggatgac cagggcgacg gtcatcgcct tggtcgccgc
      121 cgccccgaca tagccgagca ccgtctcgaa cgccgacagg ggcgccgaca ggatttcgta
      181 gatggtgccg gtgaagcgcg gcatgtggat cccgaagctg gcgttgaaga tgctctcggt
      241 aaacagcgac agcatcatca gccccgggac gatgaagctt ccgtagtcga ttccgccgat
      301 ctcgctcatg cggctgccga tcgcggcccc gcagacgatg aaatagagcg aggtggtgat
      361 caccggcacc gcgaggccgg tccacaaggt gcgcccgaag cggtgcatct cgaacttgta
      421 gatcgcccag atcccgcgcc agttcattgg gcggcgctcc tgtgttcgtc cctttgctcg
      481 tggatcaggc cgacgaagat gtcctcgagg ctcgactgct tggtgtcgag gtccttgaag
      541 gcgatgccgg cgtcgcgcat cgccgacagg agcgacggga ttcccgtgcg ctcggcccgg
      601 gcgtcgaact cgtaggtcag agtgttgccc tcgtcgctga gctgcggctg ccagtcggca
      661 agctccggcg ggacggccgc gagcggctcg gcgagctgca ggtggagcac cttgcgcccg
      721 agcttttcca tcagcgcgtg cttctcctcg accaggatca gctcgccctt gttgatcacg
      781 cccacccggt cggccatctc ctcggcttcc tcgatgtaat gggtggtgag gatgatcgtc
      841 gtgccgtcct cgcgcagccg gcggaccagc ccccacatgt cgcggcggag ttcgacgtcg
      901 accccggcgg tcggctcgtc gaggaacaag atgtcgggct cgtgggcgag cgccttggcg
      961 atcatcacgc ggcgcttcat gccgccggac agttccttga ggatctgctt gcgcttgtcc
     1021 cacagcgaca ggtcgcggag gatccgctcg accttggcgc ggtcgggggc gcgcccgaac
     1081 agctcgcggc tgaagctcac cgtgctgatc aaaggctcga acacgtccat cgtcagctct
     1141 tgcgggacga ggccgatccg ggtgcgcgcc tcgcggtagt tgcgaagcca gtgcttgccg
     1201 tcgaccagca ccgtcccgcc gctcggggtg acggtgccgc agacgatgtt gatcagggtc
     1261 gtcttgccgg ctccgttggg gccgagcagg gcgaagatct cgccgcgctc gatgtcgagg
     1321 tcgaccgatt tcagggcctc ggtgccgctg gcataggatt tgcgcagccc gcgaatgctg
     1381 aggatcgtcc cgctcattct acgggcccgg gctcggccgg ctgggcctcg tcgcgcgcca
     1441 ggccgtggcg gagcagcatc ggcacattgg cggccgcgaa caggaaggtg agggggatcg
     1501 cgccccacag cttgaacccg acccagaagt cggtcgagct gttgcgccac acggcttcgt
     1561 tgagcatcgc catgccggcg aagaacaggg cccagttgcg ggtcagcttg gtccagcccg
     1621 cgctgtcgag ccccggatag gtgctcccga gcaccgcttt cagcaacggc cggccggtcg
     1681 cgaggccgaa cccgagcagc cccgacacca gcagataata gatcgtcggc ttgatcttga
     1741 tgaaggtctc gttgcgaagc cagatggtca gcccgccgag cacgatgacc atgaatccgg
     1801 aaaacagcag cagcggcgag atccggcgaa agcgaagctg cgagtagatc atcgcggcga
     1861 tcatcgccgc catgaacgcc cccgtcgcga cgaagattcg cagcacgtcg ggcaccgggg
     1921 ccagataatt ggtcaggaag aaaatcagca gcgggccgag gtcgatcagc agccgggtgc
     1981 cgccgctcgg ttcgcgcggg tcgctcatcg tggccttgtt cattggggca gtccggcgat
     2041 ggcccgggcc acggcgcgcg gatcgaacgg gcgcaaatcg tcgacgccct cgccgacccc
     2101 gatcgcgtgg atcggaagcc cgaacttctc ggccgcggcg accaggatgc cgccgcgcgc
     2161 ggtcccgtcc agcttggtca tgatcagccc cgtcaccccc gcgctgtctc ggaacacctc
     2221 gacctgcgca agcgcgttct ggccggtggt cgcgtcgagc accagaagca cgtcatgtgg
     2281 cgcctcgggg ttgatccgcc cgagcacccg ccggatcttc gacaattcgt ccatcagatg
     2341 ggtcttgttc tggagccgcc cggcggtgtc gacgaccagc acgtcgatgc cggtctcggt
     2401 cgcctggcgg accccgtcga acacgatccc cgccgcgtcg ccgccctcct tgccggcgat
     2461 caccggggcg ccgatccgct gcccccagat ctgcaactgc tcgatcgccg ccgcccggaa
     2521 ggtgtcgccg gcggccagca ggacgccata gtcctgctcc tgcagccagt ggccgagctt
     2581 gccgatggtg gtcgtcttgc ccgagccgtt gacgccgatc accagcacca cgtgcggccg
     2641 cgggaagccc cacacctcga gcggcttcgc caccggcgcg aggatggcct cgatctcctc
     2701 ggcgaggatc gtccggagcc cgcgctcgtc caattgctcg aagcggcgcg tggcgatcgc
     2761 ctcacgaatc cggcgcgacg cctccgggcc gaggtccgag gcgatcagcg cctcctcgat
     2821 gtcgtcgagg gtggcggtgt cgagcgcggc ggtggtggtc agcccggtga ggttctcgcc
     2881 gagccgcgcc gccgtcttgg tgaacccgcc gcgcaggcgg tcgagccaac tcatgcgcag
     2941 cagcccacga gatggtcgcc gtcgcggccg gtgattcgcg cgtcgcggat ctcgccgcgc
     3001 tcggcgccct cgatccggac cggcgcgaag ccgtcgctgt ggcccttgcc ggggctttcg
     3061 accagcaccc gctgggtggt gccgatgagg ctgtcgagcc acgcgctccg ccgcgccgcg
     3121 gccgcttcgc gcagccgggc cgcgcgcgcc ttgacggttt cgcggtcgac ctgcggcatc
     3181 cgcgccgccg gggtccccgg ccgcggcgag aaggggaaca cgtgcgcggc gatgacgtcg
     3241 caatcgtcga gcagcttgag gctgttcaga gccatgtcct cggtctcggt cggaaagccg
     3301 gcgatcagat cggcgccgat ggtggcatcg ggccgcgatg ccttgatccg ctcgaccgtc
     3361 cgaaccgcgt cggcgcggct gtggcggcgc ttcatgcgct tcaggatgag gtcgtcgccg
     3421 gcctgcagcg acagatggaa atgcggcatc agccgcggct cgccggcgat caggtccatc
     3481 agctcatcgt cgatctcgac gctgtcgagc gacgacaggc gcagccgctc gaggcgcggc
     3541 tcggcggcga gcagccgccg gcacagcgat ccgagcccgc cgcgataatc ggtgatgtcg
     3601 accccggtca aaacgatctc cttcgcgccg cggtcgatct cgcgggcgac ggcgtcgcgg
     3661 atcgcctcga aggggaagga gcggctcggc ccgcgagcct gccagatgga gcagaaggtg
     3721 cagcggtggt cgcagccggt ctggatgccg acgaagctgc gcacccgcgc atgggcaccg
     3781 gcgggaacgg ccggcggcgc cgcgaagtcg cgcatccgga tctcgtccac ctcgggcatt
     3841 gcgcgaaagc ggtcgggctc gagctgcacc gcgcacccgg tcgccaggat cttcgcgccg
     3901 gggcgctcgc gccgggcgcg gcggatcgcc tgccgcgact gccgcaccgc ctcggccgtc
     3961 accgcgcagc tgttgacgat cacccagtcg tccttgccgg gcacgagccc gcgcatcgcc
     4021 tcgctctcgg cgaaattcag gcggcagccg agcgtgatcg tctcgacgct caccccttgg
     4081 accccccggt cagcttctcc aggtcgatct cgccccggaa cacataggtc gcgggcccac
     4141 gcatccggac cggctcgccg ggttgccagg cgatggtcag cgaccctccc ggcatcgtca
     4201 ccttgaccgg cgattgcgcc cgcctggagg cgatcgccgc caccgccgcc gcgcaggcgc
     4261 cggtgccgca ggcgagtgtc agcccggcgc cgcgctccca ggtccgcagc cggattccgc
     4321 cgccgccgac ttgggcgacg ctgacgttga tccggtcggg gaaggcgggg tcgtgctcga
     4381 tcagcggccc gagcccttcg agcggaatgg cctccgcatc ctcgacgaag aacaccagat
     4441 gcgggttgcc gacgctcagg gccgtcggca tttcgagctc accccacgcc atcggcagag
     4501 cggcggtgtc catcgcatag gccagtggca cctcgtccca gccgaaccgc ggctcgccga
     4561 tcgccacctc gacctcgctt ccgtccgccg ccccgctgac cagcccgccg tcggtctcga
     4621 tgctcgtcgc gccggtcaga gcgacgacgc agcgcgccgc attgccgcag ctttcgacct
     4681 cgccgccgtc gtggttccag atccgcatgc gcacgtccgc cgtgtccgac ggctcgagca
     4741 cgatcagctg gtcgcagccg atgcccttgt ggcggtcggc gagcgcccgg gcgagcgcct
     4801 cggacatcgc cagcggctcc tcgcgcgcgt cgaagatcgc gaagtcgttg ccgagcccct
     4861 ccatcttgtg aaaaggcatc ttgtgaaagc cgtgcgccat gggtcgccgc gcacttaggc
     4921 agcgcctcgg cccaagtcca cttcaccttg acctcgacaa gccccacgtc tagggcgcca
     4981 gcgtcgtttt tcgacacata gcgatgagca gcgcccaagg gtgcacgctg gccggcgagg
     5041 tttcgcccgc cggcgcattt gcatttgaag accggttcgt cggcgggaga ttgggatgtt
     5101 cgacagcctg agcgatcggc tcagcagcgt attcgacaag cttcgcgggc gcggcgcgct
     5161 cagcgaggcc gacgtgcgct cggcgatgcg cgaggtgcgg gtcgcactgc tcgaggccga
     5221 cgtcgctctg ccggtcgccc gcgacttcgt cgaccaggtg acggagcgag cggtcggcca
     5281 ggaggtgctg cgctcggtca cgcccggcca gatggtcgtc aagatcgtcc acgacgccct
     5341 ggtcgggatg ctcggctcgg aagcgtccga gctgcgcctc ggcgtcaacc cgccggcggt
     5401 gatcctgatg gtcggcctgc agggctcggg caagacgacg accaccgcca agctcggcaa
     5461 gcggctcgcg gagcgcgagc gcaagaaggt gttgatggcc tcgctcgacg tcgcccgccc
     5521 ggccgcccag gcgcagctgg cggtgctcgg ccgccaggcg aatgtcgaca cgctgccggt
     5581 cgtcgccggc cagcagccgg tcgatatcgc caaacgcgcg atccaggcgg ctcgcctcca
     5641 ggcctatgac gtcgtcctgc tcgacaccgc cgggcgcctg cacgtcgacg atgcgctgat
     5701 ggccgagatg aaggcggtcg ccgcggcctc cagcccgacc gagacgctgc tggtggtcga
     5761 cgcgctgacc ggccaggacg cggtcaacgt cgccaggggc tttgccggcg aggtcgatct
     5821 gaccggcgtg atcctcaccc ggctcgacgg cgacgcccgc ggtggagcag ctttgtcgat
     5881 gcgcgcggtg accggcaagc cgatcaagtt cgccggggtc ggggaggcga tcgacaagct
     5941 cgaggcgttc cacccggacc gagtcgccgg gcggattctc ggcatgggcg acgtcgtcag
     6001 cctggtcgag cgcgccgccg agaccatcga ggtcgaggag gccgagaagc tcgcggccaa
     6061 gatggtcaag ggcaagttcg acctcgacga cctccgcgcc cagatccagc agatgcagcg
     6121 catgggcggg ctgggcgcgc tggcctcgat gatgccgggc ctgaagaccg tgaagggcgc
     6181 ggccgagaag gccgccgacg gcaaggcgct cgtccatctg gaagcgatga tgtcgtcgat
     6241 gaccgccaag gagcgctcgc gccccgagct gatcaacgcc aagcgcaaga ttcgcatcgc
     6301 caagggctcg gggaccaccg tccaggaggt caacaagctt ttgaagatgc accaggaaat
     6361 ggcgagcgtg atgaagcggc tgaagaaaat gggcgggctc ggcaagctcg ccgcgatgtt
     6421 cggcaagggc ggcctcgaag gcgcgctcgg cggggtcgcg cccggaatgc tcccgcccgg
     6481 cgccctcggc ggcggcgggc cggctttgcc ggggctcggc ggctcgccgt tcaacctccc
     6541 gcccggtttc gacaagttca caaagaagta aacgaactca cggacaagga acgataatcc
     6601 atggcacttt ccattcgact gacccgcggc ggcgccaaga agcggcccta ttaccgaatc
     6661 gtcgtcgccg acagccgcgc gccgcgcgac ggccgcttca tcgagaagct cggcagctac
     6721 aacccgctgc tggccaagga ttcgcccgaa cgggtgaagc tcgacgccga gcgcattgcg
     6781 cactggctgt cggtcggcgc ccagccgtcg gaccgagtgc tgcgcttcct cgacgcggcc
     6841 ggcatccgcg agcggccggc gcgcaacaac ccgaagaagg gcgagccggg cgaaaaggcc
     6901 aaggcgcgga tcgaggagcg ggccggcaag gcagccgcgg ccgccgaggc cgccgaagct
     6961 gctgcggcag ctccg
//
LOCUS       DASTZO010000115         5632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1027888,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000115 DASTZO010000000
VERSION     DASTZO010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5632
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027888"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VFU20_03250"
     CDS             <1..722
                     /locus_tag="VFU20_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HEU5285513.1"
                     /translation="TGIATPSLEARVGPKVGLRPDIRRVPVFGLGCAGGVNGLAIASR
                     LANADPGSNWLFVTVETCSISIRLDSDDPSAIVATALFGDGAAAAVVGDGEPGLARIG
                     GSAEKLWPDTLRIMGWDVEDPGLAVVFDRAIPPFIEAELADAVDAMLRALELERGDIA
                     RFCCHPGGVKVIDAIETALALSQGELDLEREVLRDFGNMSAPTVLFVLDRLIARGLPG
                     RVLMTAFGPGFTCAGCLLEAP"
     gene            719..1228
                     /locus_tag="VFU20_03255"
     CDS             719..1228
                     /locus_tag="VFU20_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU5285514.1"
                     /translation="MTWTAFWIGIAILGFVTLQRLIELRLAERNSKRLLAEGAKEHAA
                     GHYPFLVAIHMAWLAALWWWAPGRPVDVPLLLLFAALQVGRIWVIATLGRRWTTRIIV
                     LPGAPLVRSGPYRYLSHPNYVIVTAEIAVLPLVFGLVEIAVAFSILNLAVLAVRIPQE
                     NKALDRLRR"
     gene            1337..1873
                     /gene="rimP"
                     /locus_tag="VFU20_03260"
     CDS             1337..1873
                     /gene="rimP"
                     /locus_tag="VFU20_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation protein RimP"
                     /protein_id="HEU5285515.1"
                     /translation="MVDLAALTGLIEPEVEALGYRLVRVAMIGGASDPTLQVMAERPD
                     TRQLQLGDCERISKRLSEVLDVLESEGQDPIEGGYRLEVSSPGIDRPLTRPSDYADWA
                     GHEARIKLHETLGGAKQLSGTIQGIDKGIVLLSTPKGDREIPFESIASAKLLLTDALI
                     KATAPLDAEGADLIKMEG"
     gene            1880..3511
                     /gene="nusA"
                     /locus_tag="VFU20_03265"
     CDS             1880..3511
                     /gene="nusA"
                     /locus_tag="VFU20_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU5285516.1"
                     /translation="MATAAPASVSANKAELLAIADAVAREKLIDRGIVIEAMEDAIQR
                     AARARYGAENDIRAKLDPQTGDLRLWRVVEVVEEVDDYFKQVDLKGAQKLKADAQIGD
                     FIVDPLPPIEFGRIAAQAAKQVIFQKVRDAERERQYEEFKDRAGEVITGVVKRVEFGH
                     VVVDLGRAEGVIRRDQQIPREMVRVGDRIRSLILSVRREARGPQIFLSRAHPDFMKKL
                     FAQEVPEIYDGIIEIKAAARDPGSRAKIGVISHDSSIDPVGACVGMKGSRVQAVVQEL
                     QGEKIDIIPWSEDLATFVVNALQPATVSRVVIDEEEARIEVVVPDDQLSLAIGRRGQN
                     VRLASQLTNSQIDILTEADASEKRQREFVERSEMFQNELDVDETLAQLLVAEGFTTLE
                     EVAYVNAEEISAIDGFDEDLAAELQSRAGEALERREAAAREERRKLGVDDALAELPHL
                     TEAMLVTLGRANIRTLDDLADLATDELIQKKRPEQRRQREPSNRPEDRGGILHEYGLT
                     EEQGNEIIMAARAHWFEDEDKASSGEEDAVAETGQ"
     gene            3621..4265
                     /locus_tag="VFU20_03270"
     CDS             3621..4265
                     /locus_tag="VFU20_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF448 domain-containing protein"
                     /protein_id="HEU5285517.1"
                     /translation="MLTRRKGTRDGLVRLALGPDGAVGPDVRARAPGRGAWISVGRAE
                     LDAANAKGKLKGALARAFKAADLKIPADLGDRIERALRQAALDRLGLEARGGTLINGA
                     ERIETAARAGKVHLLIHAADAGEDGRRALDQAWRTGGGQPRGLLFPEERSILSMALGR
                     ENVVHVALTDPAAASRVSHALARWRAFTDPRTGLDGGEPASAPGSAEEFDEGTE"
     gene            4262..>5632
                     /locus_tag="VFU20_03275"
     CDS             4262..>5632
                     /locus_tag="VFU20_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
                     /protein_id="HEU5285518.1"
                     /translation="MTDQTEKPKLGTRPPLGIKRTVETGKVKQSFSHGRSNTVVVEVK
                     KRRILGKPGEPAPAEAPKPAPAAEAPKPAAPTRPAPAPAPRRAPADDITTRRELQEKL
                     LREADEARMAALEEARRRDERVKVEQTEDEKRRAEENRRAEEEAAEQARTREAEPQVE
                     AAAEPAPVVEERVPELEDEEERGFRRPGAPAPKRPEPARPPRNREDRRHSGKLTVTRA
                     LSGEDDSRARSLAALRRAREKEKRHHQQAGPAAKQVRDVVVPEAITVQELANRMAERG
                     ADLVKALFKMGMPVTLTQTIDQDTAELLVTEFGHNIKRVSEGDVEIGIGDVEDSPESL
                     QPRPPVVTIMGHVDHGKTSLLDAIRGADVVSGEAGGITQHIGAYQVTVPDKSKVTFLD
                     TPGHEAFTEMRARGANVTDIVVLVVAADDGIRPQTIEAINHTKAAGVPMIVAINKVDK
                     PDANPQR"
BASE COUNT          932 a         2025 c         1934 g          741 t
ORIGIN      
        1 cgaccggaat cgccaccccc agcctggaag cgcgagtcgg tccgaaggtc ggcctcaggc
       61 ccgacatccg ccgcgtgccg gtcttcggcc tcggctgtgc cggcggggtc aacggcctcg
      121 ccatcgcctc acgcctcgcc aatgccgatc ccggctcgaa ctggctgttc gtcaccgtcg
      181 agacctgctc gatctcgatc cgcctcgaca gcgacgaccc gtcggcgatc gttgccaccg
      241 ctttgttcgg cgacggcgcc gccgcggcgg tggtcggcga cggagagccg ggactcgcgc
      301 ggatcggcgg gtcggccgag aaactgtggc ccgacacgct tcggatcatg ggctgggacg
      361 tcgaggaccc cgggctggcc gtggtgttcg accgggcgat cccgccgttc atcgaagccg
      421 agctcgccga tgcggtcgac gccatgctgc gcgcgctgga gctggagcgc ggcgacatag
      481 cgcgcttctg ctgccacccg ggcggggtca aggtgatcga cgcgatcgaa accgcgctcg
      541 ccctgtccca gggagagctc gacctcgagc gcgaggtcct tcgcgatttc ggcaacatga
      601 gcgccccgac ggtgctgttc gtgctcgacc ggctgatcgc ccgcggcctg cccgggcgcg
      661 tgctgatgac cgcgttcggc cccggcttca cctgcgccgg ctgcctgctg gaagcgccat
      721 gacctggacg gcgttctgga tcgggatcgc aatcctcggc ttcgtcaccc tgcagcggct
      781 catcgagctg cgcctcgccg agcgcaacag caagcgcctg ctcgccgaag gcgcgaagga
      841 gcatgcggcc ggccattatc cgttcctcgt cgcgatccac atggcgtggc tcgctgcgct
      901 ctggtggtgg gctccggggc ggccggtcga tgtcccgctt ctgctgctgt tcgcggcgct
      961 ccaggtcggc cggatctggg tgatcgccac gctcggccgg cggtggacga cgcggatcat
     1021 cgtccttccc ggagcgccgc tggtccgatc gggtccctac cgttacctga gccaccccaa
     1081 ttatgtgatc gtcaccgcgg aaatcgccgt cctgcccttg gtgttcggcc tggtcgagat
     1141 cgcggtcgcg ttctcgatcc tcaacctggc cgtcctcgcc gtccgcatcc cgcaggagaa
     1201 caaggcgctc gaccgcttgc gccgctagcc ggacgcgcct atatcgccgc ctctcccctc
     1261 gacatcgcta gcgacgttga ggctggtctc cacccggggg ccggtcggag tgctgttttg
     1321 gttttcaaag gacgtcatgg tcgacctggc cgcgcttacc ggactgatcg agcccgaggt
     1381 ggaagccctg ggctaccggc tggtgcgagt cgcgatgatc ggcggggctt cggacccgac
     1441 gcttcaggtg atggccgagc ggccggacac ccggcaactt cagctcggcg actgcgagcg
     1501 gatttcgaag cggctgtcgg aagtgctcga cgtcttggag agcgaggggc aggacccgat
     1561 cgagggcggc taccgcctcg aggtcagctc gccgggcatc gaccggccgc tgaccaggcc
     1621 gtccgattat gccgactggg cggggcacga ggcgcgcatc aagctccacg agactttggg
     1681 cggcgccaag caattgtccg ggaccatcca gggcatcgac aagggcatcg tcctgctctc
     1741 gacccccaag ggcgaccgtg aaatcccgtt cgaatccatc gcttcggcca agcttctgct
     1801 gaccgacgcg ctcatcaagg ccaccgcccc gctcgacgcg gaaggggccg atctcatcaa
     1861 gatggaaggc taaattccca tggctaccgc cgctcccgcg tccgtatccg ccaacaaggc
     1921 cgagctgctc gcgatcgccg acgccgtcgc gcgcgagaaa ctgatcgacc gcggcatcgt
     1981 catcgaggcg atggaggacg ccatccagcg cgccgcccgc gcccgctacg gcgccgagaa
     2041 cgacatccgc gccaagctcg acccgcagac cggcgatttg cgcctgtggc gcgtggtcga
     2101 ggtggtcgag gaggtcgacg actatttcaa gcaggtcgac ctgaagggcg cccagaaatt
     2161 gaaggccgac gcccagatcg gcgatttcat cgtcgacccg ctgccgccga tcgagttcgg
     2221 ccggatcgcc gcccaggccg ccaagcaggt gatcttccag aaggtccgcg acgccgagcg
     2281 cgagcgccag tacgaggagt tcaaggaccg cgccggcgag gtcatcaccg gggtcgtcaa
     2341 gcgggtcgag ttcggccacg tcgtcgtcga cctcggtcgg gccgagggag tcatccgccg
     2401 cgaccagcag atcccgcgcg agatggtccg cgtcggcgac cggatccgga gcctgatcct
     2461 gtcggtccgc cgcgaggccc ggggcccgca gatcttcctg tcgcgcgccc accccgactt
     2521 catgaagaaa ttgttcgctc aggaagtgcc cgagatctac gacgggatca tcgagatcaa
     2581 ggccgccgcc cgcgacccgg gcagccgcgc caagatcggc gtcatcagcc acgacagctc
     2641 gatcgacccg gtcggcgcct gcgtcgggat gaagggcagc cgcgtccagg cggtggtcca
     2701 ggagctccag ggcgagaaga tcgacatcat cccgtggagc gaggatttgg cgaccttcgt
     2761 cgtcaacgcg ctccagccgg cgaccgtcag ccgagtcgtc atcgacgagg aagaggcgcg
     2821 gatcgaggtg gtcgtccccg acgaccagct gagcctggcg atcggccgcc gcggccagaa
     2881 cgtccgcctg gcgagccagc tgaccaattc gcagatcgac atcctgaccg aggccgacgc
     2941 aagcgagaag cgccagcgcg agttcgtcga gcgctccgag atgttccaga acgagctcga
     3001 cgtcgacgag acgctcgccc agctgctggt cgccgagggc ttcaccactc tcgaggaggt
     3061 cgcctacgtc aacgcggagg aaatcagcgc gatcgacggc ttcgacgagg atctcgccgc
     3121 cgagctccag agccgcgccg gcgaggcgct ggagcggcgc gaggccgcgg cccgcgagga
     3181 gcgccgcaag ctcggcgtcg acgacgcgct cgccgagctg ccgcacctga ccgaggcgat
     3241 gctcgtcacc ctggggcgcg cgaacatccg caccctcgac gacctcgccg acctcgccac
     3301 cgacgagctc atccagaaga agcggcccga gcagcgccgc cagcgcgagc cgagcaaccg
     3361 gcccgaggac aggggcggca tcctgcacga gtacggcctg accgaagagc agggcaacga
     3421 gatcatcatg gccgcccgcg cccactggtt cgaggacgag gacaaggctt cctccggcga
     3481 ggaggacgca gttgcggaaa ccgggcaatg aggccgcgct gagccgttcc ccggcgaaat
     3541 ccggggccca gcccaagccc agagcaaccg gaccccggcc ttcgccgggg accggtccgc
     3601 acgtccccga gcggacctgc gtgctcaccc gccgcaaggg cacgcgcgac ggcctagtcc
     3661 ggctggcgct gggcccggac ggcgcggtcg gccccgatgt ccgcgcccgg gcgccgggcc
     3721 gcggcgcgtg gatttcggtc ggccgcgccg agctcgacgc cgccaacgcc aagggcaagc
     3781 tcaagggcgc gctggcccgg gcgttcaagg ccgcggacct caagattccc gccgacctcg
     3841 gcgaccggat cgagcgcgcg cttcgccagg cggcgctcga ccggctcggc ctcgaggcgc
     3901 gcggcggcac cctgatcaac ggcgccgagc gaatcgagac ggcggcccgc gccggcaagg
     3961 tccatctgct gatccacgcc gccgatgccg gcgaggacgg ccgcagggcg ctcgaccagg
     4021 cgtggcgcac cgggggcggt cagccgcgcg gcctcctatt tcccgaggag cgctcgatct
     4081 tgtcgatggc cttggggcgc gaaaatgtgg tacatgtcgc cctgaccgat cccgccgccg
     4141 cgtcgcgcgt gtcccacgcg cttgcccggt ggcgcgcttt cactgacccc cgtaccgggc
     4201 tagacggcgg cgaacccgcc tcggcccccg gctcggctga agaatttgac gaaggaacag
     4261 aatgaccgac cagaccgaaa agccgaagct tggcacccgg ccgccgctgg gcatcaagcg
     4321 cacggtcgag accggcaagg tcaagcagag cttcagccat ggccggtcga acaccgtcgt
     4381 ggtcgaggtc aagaaacgcc ggatcctcgg caagccgggc gaaccggccc cggccgaggc
     4441 gcccaagccg gcgcccgcgg ccgaggcgcc gaagccggcg gccccgaccc gcccggcgcc
     4501 cgccccggcg ccgaggcgcg ccccggccga cgacatcact acccgacgcg agctccagga
     4561 aaagctgctg cgcgaggccg acgaggcgcg catggcggcg ctcgaggaag cccgccgccg
     4621 cgacgagcgg gtcaaggtcg agcagaccga ggacgagaag cgccgggcgg aggaaaaccg
     4681 ccgcgccgag gaagaggcgg ccgagcaggc gcgaactcgc gaggccgagc cgcaggtcga
     4741 ggcggccgcc gagcccgcgc cggtcgtcga ggagcgcgtt cccgagctcg aggacgagga
     4801 ggagcgcggt ttccggcgcc ccggagcacc ggcgcccaag cgccccgaac cggcacggcc
     4861 gccgcgcaac cgcgaggacc gccgccattc gggcaagctg accgtgaccc gcgccctgtc
     4921 cggcgaggac gacagccgcg cccgttcact ggccgccctg cgccgcgccc gcgaaaagga
     4981 aaagcgccac caccagcagg ccggcccggc cgccaagcag gtccgcgacg tcgtcgtccc
     5041 cgaggcgatc accgtccagg agctcgccaa ccggatggcc gagcgcggcg ccgacctggt
     5101 caaggcgctg ttcaagatgg gcatgccggt gaccctcacc cagaccatcg accaggacac
     5161 cgccgagctg ctggtcaccg agttcgggca caacatcaag cgggtcagcg agggcgacgt
     5221 cgagatcggc atcggcgatg tcgaggattc gcccgagagc ctccagccgc ggccgccggt
     5281 ggtgacgatc atgggccatg tcgaccatgg caagacgtcg cttctggacg cgatccgcgg
     5341 cgccgacgtc gtctccggcg aggccggcgg gatcacccag cacatcggcg cctatcaggt
     5401 gaccgtgccc gacaagtcga aggtcacctt cctcgacact cccggccacg aggcgttcac
     5461 cgagatgcgc gcgcgcggcg ccaacgtcac cgacatcgtc gtgctggtgg tcgcggccga
     5521 cgacggcatc cgcccgcaga ccatcgaggc gatcaaccac accaaggctg ccggcgtgcc
     5581 gatgatcgta gcgatcaaca aggtcgacaa gccggatgcc aatccgcagc gc
//
LOCUS       DASTZO010000116         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_791654,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000116 DASTZO010000000
VERSION     DASTZO010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791654"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VFU20_03280"
     CDS             <1..445
                     /locus_tag="VFU20_03280"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5285519.1"
                     /translation="VLTLATAAAAVPSGWLADRIGRPAVMLAGGAIGAVGVAALPLAG
                     SLPAILVFGSLMAVGSAAFGAGSWAALADATAERSTGRLLGIANLGTAGAAAAAGLFG
                     PLIDAAGFGPAFALAGVLAAAGALVGWRAGASAPRPSLRPIEVFD"
     gene            445..>2015
                     /locus_tag="VFU20_03285"
     CDS             445..>2015
                     /locus_tag="VFU20_03285"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5285520.1"
                     /translation="MLRRLCFDVPTLLSVLYAPLQPLVKRLRIVRRGDRRYQPNDVLL
                     PPGYGAEVVATGLTAPVHASFGPHGAMYVTESGHKSESPPRIFEVDPTSGSRRLVVEL
                     SGPHAQSTGAATGTAWHDGSLIVTATDAVLRVDPETGVTTELVDGLPGLGDHQTNHPV
                     IGPDGRVYWGQGCVTNMGVVGADNFGYEWLAKFPDQHDVPGGDIVLAGRNYEVPNVLG
                     NPLERVTTGAYVAFGTATEPNQRIPGSPKASGAVLSCLPDGSDLRVVAWGLRNPYGIA
                     VHPDGRLFVTEHGSDERGARWIVGDPDDLYEIREGAWYGWPDFASGIRLDDPHWGPGG
                     TGREPVLAEFPDPEPPKPVASFEPHAAANGFDFSRDPAFGFEGQAFVALFGDLAPVTT
                     PRQVVPAGFKIVRVDPSTGAIHDFAVNRIQGPASKLFHGGFERPSHCVFGSDGALYVV
                     DFGEIKIAPEKGAIRIKQLTGAVWRIRRVEGAAAGEKPPDQVRVPFYPLQTIALVGVV
                     AAVVFGVVALVRGLLR"
BASE COUNT          241 a          739 c          721 g          314 t
ORIGIN      
        1 ggtcctcacc ctcgcgaccg ccgccgccgc cgtcccgtcg ggatggctgg cggaccgcat
       61 cgggcgaccg gcggtgatgc tcgccggggg tgcgatcggc gcggtcggcg tcgccgcgct
      121 gccgctggcc ggctcgctgc cggcgatcct ggtcttcggc tcgctcatgg ccgtcgggtc
      181 ggcagccttc ggcgccggga gctgggccgc cctggccgat gcgaccgccg aacgctccac
      241 cggccggctg ctcggcatcg ccaacctggg caccgccggc gcggccgccg ctgcgggcct
      301 gttcgggccg ctgatcgatg ctgccgggtt cggcccggcc ttcgccctcg cgggagtgct
      361 ggcggccgct ggcgcgctcg tcgggtggcg cgccggcgca tcggctccgc gaccgtcgct
      421 ccgtcccatc gaggtcttcg actgatgctc cgccgtctct gcttcgacgt tccgacattg
      481 ctctcggtgc tgtacgcgcc gctccagccg ctggtcaagc ggctccgcat cgtgcggcgg
      541 ggggatcggc gataccagcc taacgacgtc ctgctgccgc cgggctacgg cgccgaggtg
      601 gtcgccaccg gcttgacggc tcccgtgcat gcctccttcg gtccccatgg cgccatgtac
      661 gtgaccgaat cggggcacaa gtcggagtca ccgccgcgca tcttcgaggt ggatccgacg
      721 agtggcagcc ggcgcctggt ggtcgagctc agtggcccgc acgcgcagtc gacgggggcc
      781 gcaaccggca ccgcgtggca cgacggttcg ctgatcgtca ccgccaccga tgcggtcctg
      841 cgcgtcgacc ccgagacggg cgtcaccacc gagctcgtcg atggcctccc ggggctcggg
      901 gaccaccaga cgaaccaccc ggtcatcggc cccgacggcc gcgtctactg gggccagggc
      961 tgcgtcacca acatgggcgt cgtcggcgcc gacaacttcg gctacgagtg gctggccaag
     1021 ttcccggatc agcacgacgt gccgggcggc gacatcgtgc tggccggccg caactacgag
     1081 gtgccgaacg tcctcggcaa cccgctcgag cgcgtgacga cgggcgcgta cgtggcgttc
     1141 ggtaccgcca cggagcccaa ccagcgcatc ccgggcagcc cgaaggccag cggcgcggtg
     1201 ctcagctgcc tgcccgatgg atcggacctg cgcgtcgtcg cctggggcct gcgcaacccg
     1261 tacgggatcg ccgtccaccc cgacggccgc ctgttcgtga cggagcacgg gtccgatgag
     1321 cgcggcgccc ggtggatcgt cggcgacccc gacgacctct acgagattcg cgagggcgcc
     1381 tggtacggct ggccggattt cgcctccggc atccggttgg acgatccgca ctggggtccg
     1441 ggcgggaccg gccgggagcc ggttctggcc gagtttccgg atcccgagcc gcccaagccg
     1501 gtcgcctcgt tcgagcccca cgcggcggcc aacggcttcg atttcagccg cgatccggcc
     1561 ttcggcttcg agggccaggc gttcgtcgcg cttttcggcg accttgcgcc ggtcaccacg
     1621 ccgcgccagg tcgtgccggc cggcttcaag atcgtgcgcg tggatccgtc gaccggcgcc
     1681 atccacgact tcgcggtcaa ccgcatccag ggccccgcct cgaagctgtt ccacggcggc
     1741 ttcgagcggc ccagccactg cgtcttcggg tccgatggcg cgctgtacgt cgtcgacttc
     1801 ggcgagatca agatcgcgcc ggagaagggc gccatccgga tcaagcagct gacaggcgcg
     1861 gtctggcgca tccgccgcgt cgagggcgcc gcggcaggcg agaagccacc ggaccaggtg
     1921 cgagtcccgt tctatccgct gcagacgatc gcgctggtgg gagtggtcgc ggccgtcgtc
     1981 ttcggagtgg ttgcgctggt gcgtgggtta ctgcg
//
LOCUS       DASTZO010000117         2410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1158733,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000117 DASTZO010000000
VERSION     DASTZO010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2410
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1158733"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VFU20_03290"
     CDS             complement(<1..1124)
                     /locus_tag="VFU20_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU5285521.1"
                     /translation="MTDRLIIALAQMSQRVGDLEGNAAAMLAMRDKAAGADLLLCPEL
                     QLTGYPPEDLVLKPEFVRRTMESAERLVDATASPGPAMLIGTIVNDGGANYNAVLLAD
                     GGRVVGRTLKRELPNYGTFDEKRVFAHGPMPEPIEFKGVKIGVPICEDIWQEPVCAHL
                     AEAGAEMLLVPNGSPYELDKDDLRQRMVRSRAAITGLPLAYLNRVGGQDEIVFDGSSF
                     VMHPDGELVVQMPDWDEHLLLTEWRRGPDGWRCETRAEHRLDPFPEDVYRAMVVALRD
                     YVTSNGFPGVILGLSGGIDSALSAAVAVDALGPDKVWGVMLPSRYTSPESLEDAAECA
                     RLLGCRHDVVSIAPAVEAMDGMLAPHRVGNTPGLAEENIQA"
     gene            1222..2157
                     /locus_tag="VFU20_03295"
     CDS             1222..2157
                     /locus_tag="VFU20_03295"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU5285522.1"
                     /translation="MKLLAGNSNLPLSRSISEYLEMPLTEASVRRFADEEIFVEINEN
                     VRGEDVFVIQSTSYPANDNLMELLICIDALRRASAKRITAVLPYFGYARQDRKPGPRT
                     PISAKLVANLITVAGASRVLSIDLHAGQIQGFFDIPTDNLFASPVIAADIQARFSNRN
                     LTVVSPDVGGVVRARGLAKRLSNAPLAIVDKRREKAGESEVMNIIGDVDGRSCILIDD
                     IVDSGGTLCNAAAALRQQGAKEVVAYCTHGVLSGGAAARIGTSELSELVVTDTICTGE
                     ADPKDGKIRRLTIAPLLAEAIRRIADEASVSSLFD"
     gene            2159..>2410
                     /locus_tag="VFU20_03300"
     CDS             2159..>2410
                     /locus_tag="VFU20_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285523.1"
                     /translation="MNRRGGWLRPAGLFVLALLAILIAYELFAVFRARQATPRVLAEA
                     ARGELELADLPRRRLDMLIKVEDPGFYRHRGVDFSTPGAG"
BASE COUNT          371 a          840 c          814 g          385 t
ORIGIN      
        1 gcctggatat tctcctcggc gagacccggc gtgttgccga cccgatgcgg ggccagcatc
       61 ccgtccatcg cctcgaccgc cggggcgatc gacacgacgt catggcggca gccgagcagg
      121 cgagcgcact cggccgcgtc ctcgaggctt tccggcgacg tgtatctgga cggcagcatc
      181 acgccccaca ccttgtcggg gccgagcgcg tcgaccgcga ccgcggccga cagggcgctg
      241 tcgatgccgc ccgacaggcc gaggatcacg cccggaaagc cgttgctggt cacatagtcg
      301 cgaagcgcaa ccaccatcgc ccggtagacg tcctcgggga acgggtcgag ccggtgctcg
      361 gcgcgggtct cgcagcgcca gccgtcgggg ccccgccgcc attcggtcag cagcagatgc
      421 tcgtcccagt cgggcatctg cacgaccagc tcgccgtccg ggtgcatgac gaacgacgag
      481 ccgtcgaaca cgatctcgtc ctggccgccg acgcggttga ggtaggcgag cggcaggccg
      541 gtgatggcgg cccgcgaccg caccatccgc tgccgcaaat cgtccttgtc gagctcgtag
      601 gggctgccgt tgggcaccag cagcatctcc gccccggcct ccgccagatg ggcgcagacc
      661 ggctcctgcc agatgtcctc gcagatcggc actccgatct tcacgccctt aaactcgatc
      721 ggctcgggca tcggcccgtg ggcgaagacc cttttctcgt cgaaggtgcc gtaattgggc
      781 agctcgcgct tcagggtccg cccgaccacc cggccgccgt cggcgagcag caccgcgttg
      841 taattggcgc cgccgtcgtt gacgatggtc ccgatcagca tcgccgggcc cgggctcgcg
      901 gtcgcgtcga ccagccgctc ggccgactcc atcgtccgcc ggacgaactc gggcttcaac
      961 accaggtcct cgggcggata gccggtcagc tggagctcgg ggcacagcaa gaggtcggcg
     1021 ccggcggcct tgtcgcgcat cgccagcatg gcggccgcat tgccctcgag gtcgccgacg
     1081 cgctggctca tctgggcgag ggcgatgatc aaccgatccg tcatcgccgc gtgccttaga
     1141 cgggaagctt tgctttgcga accatgcttg ggctggctaa ggggtcggcg cgcaccgccg
     1201 cgctggggag ctgccgccgc catgaaattg ctcgccggga acagcaattt gccgctgtcc
     1261 cgatcgattt ccgaatatct cgagatgccg ctgaccgagg ccagcgtgcg gcgcttcgcc
     1321 gacgaggaga tcttcgtcga gatcaacgag aacgtccgcg gcgaggacgt gttcgtgatc
     1381 cagtcgacga gctacccggc caacgacaat ctgatggagc tgttgatctg catcgacgcg
     1441 cttcggcggg cgtcggccaa gcggatcacc gcggtgctgc cctatttcgg ctatgcccgg
     1501 caggaccgga agccggggcc gcgaaccccg atttccgcca agctggtcgc caatttgatc
     1561 accgtcgccg gcgccagccg ggtgctgtcg atcgacctcc acgccgggca gatccagggc
     1621 ttcttcgaca tcccgaccga caatctgttc gcctcgccgg tgatcgccgc cgacatccag
     1681 gcgcgctttt ccaaccgcaa tctgaccgtc gtctcgcccg acgtcggcgg ggtggtccgc
     1741 gcccgcggcc tcgccaagcg cctgagcaac gcgcctctgg cgatcgtcga caagcgccgg
     1801 gaaaaggccg gcgagtccga ggtgatgaac atcatcggcg acgtcgacgg gcgcagctgc
     1861 atcctcatcg acgacattgt cgattcgggc ggcacgctgt gcaacgccgc cgcggcattg
     1921 aggcagcagg gggccaagga ggtggtcgca tattgcaccc acggagtgct gtcgggcgga
     1981 gcggcggcgc ggatcggcac gtcggagctc agcgagctgg tggtcaccga caccatctgc
     2041 accggcgagg ccgaccccaa ggacggcaag atccgccggc tgaccatcgc ccccttgctc
     2101 gccgaggcga tccgccggat cgccgacgaa gccagcgtct cgagcctgtt cgactaggat
     2161 gaatcgccgc ggcggttggt tgcggccggc cggccttttc gtcctggcgc tgctggcgat
     2221 cctcatcgcc tacgagctgt tcgcggtctt tcgcgcgcgg caggcgacgc cgcgggtgct
     2281 ggccgaggcc gcacgaggcg agctggagct cgcggaccta ccgaggcggc ggctcgatat
     2341 gctgatcaag gtcgaggacc cgggcttcta tcgccatcgc ggggtggatt tctcgactcc
     2401 cggcgcgggc
//
LOCUS       DASTZO010000118         5019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_714738,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000118 DASTZO010000000
VERSION     DASTZO010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_714738"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFU20_03305"
     CDS             complement(<1..717)
                     /locus_tag="VFU20_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285524.1"
                     /translation="MTRMFLIATVAALAITAPAQARPDDERQARTEERAEKAQARAEA
                     RKAEGGGEQRVRAERPQHQERVRIERPERIERAQRIERFERPQREQRVRMERFERPQR
                     EQRVQVERFERPQREQLIRIERAERPRRERFVEPRAPRIERRERLARQERVREERVRE
                     QRVREPRIERIERIERVERVDRQARIRDRAIERRSGDFEPRQRLIRQERRDERFTRQQ
                     LRQQEKQAERLAKLQQRAERR"
     gene            981..1532
                     /locus_tag="VFU20_03310"
     CDS             981..1532
                     /locus_tag="VFU20_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285525.1"
                     /translation="MTRNILMTALATAAIAASAPALAQPGGGGGPGGGAGAGPPMTPP
                     GQQGGGPGTADYARDNIAAQQGQFGRDFAAQRQLTAEQHRALAAQRRADALALAQAAR
                     SGARIPANAAGRIREALKEDIAAWREEFDVGRREWQAMRDQWIVDRSSLTAEQWAERR
                     AAWFAARDAWIASQKAWASARRN"
     gene            complement(1601..2599)
                     /locus_tag="VFU20_03315"
     CDS             complement(1601..2599)
                     /locus_tag="VFU20_03315"
                     /inference="COORDINATES: protein motif:HMM:NF013087.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HEU5285526.1"
                     /translation="MKTDSSSPLDVILQATGGYCVARALHVAADLGVADHIGDGGASV
                     DELAAKTGADPDALGRILRLLSAHGIFSADGGTVSHNPVSETLRTDHPQSARDLARMF
                     GLPFFWETFGDLDHSVRTGRPAVEKAHPDGLWAWLAKRPEASAIFNGAMIGKSFGQVA
                     GVVESFDFSRFPRIADVGGGRGHLLQAILARYPQARGLLFEQPHVIEEVRAIASDRLQ
                     LQAGDFFADELPQSDAYILMEVIHDWTDEESVQILAAVRRAAPTGSALLLIEQLMPPT
                     PGPHWVKMLDVHMMALFAARQRSRQEYDRLAERSGFRPVGVTDTPAGISVLEYRAA"
     gene            complement(2604..4163)
                     /gene="guaA"
                     /locus_tag="VFU20_03320"
     CDS             complement(2604..4163)
                     /gene="guaA"
                     /locus_tag="VFU20_03320"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU5285527.1"
                     /translation="MTVQPAESILIVDFGSQVTQLIARRVREAGVYSEIAPFNAADEA
                     FERLKPRGIILSGGPSSVMWDDSPRAPQHFFDCGLPILAICYGQQTMAHQLGGVVAAS
                     DNREFGRAFIEIIDESALFDGLWKVGETHQVWMSHGDRVERLPDGFKIVAKSEGAPYA
                     IATDEDRRFYSMMFHPEVVHTPDGGRLLANFVRHVCGCAGDWTMGAFRDAKIAEIREQ
                     VGQGRVICGLSGGVDSAVAAVLIHEAVGEQLTCVFVDHGLMRLGEAEQVVSLFRGSYN
                     IPLVHVDASADFLGGLAGVTDPEAKRKFIGKTFIDIFEAEAAKAGGADFLAQGTLYPD
                     VIESVSFTGGPSVTIKSHHNVGGLPERMAMKLVEPLRELFKDEVRVLGRELGLPEGFV
                     ERHPFPGPGLAIRIPGEVTEERCDILRKADAIYLEEIRNAGLYDAIWQAFAVLLPVRT
                     VGVMGDHRTYDHVCGLRAVTSVDGMTADIYPLDAAFLARVATRIVNEVPGINRVVYDY
                     TSKPPGTIEWE"
     gene            complement(4480..>5019)
                     /locus_tag="VFU20_03325"
     CDS             complement(4480..>5019)
                     /locus_tag="VFU20_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007163142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU5285528.1"
                     /translation="GKVLPVGEQGEICTRGYHVMTGYNDNPEATAKAIEADGWLHTGD
                     LGRMDERGYVAITGRVKEMIIRGGENLFPVEIENAMLEHPDIGEVAVVGVPCPTYGEQ
                     VACFMRAAGARRPEPAELKAFIRERLSPQKTPQHWIWVDQWPLTGSGKIQKFKLAEQF
                     ERREAEAALAAQPQPAPSA"
BASE COUNT          732 a         1782 c         1709 g          796 t
ORIGIN      
        1 ccggcgctcg gcgcgctgct gcagcttggc gaggcgctcg gcctgctttt cctgctggcg
       61 caattgctgc cgcgtgaagc gctcgtcgcg gcgctcctgc cggatcaggc gctggcgcgg
      121 ctcgaaatcc cctgaccgcc gctcgatcgc gcggtcgcgg atcctcgcct ggcgatcgac
      181 gcgctcgacg cgctcgatgc gctcgatgcg ctcgattcgg ggctcccgca cccgctgctc
      241 gcggacgcgc tcttcgcgga cccgctcctg ccgggccagc cgctcgcggc gctcgatccg
      301 cggagcgcgc ggctcgacga accgctcgcg ccgcggccgc tcggcccgct caatgcggat
      361 gagctgctcg cgctggggac gttcaaatcg ttccacctgg acccgctgtt cgcgttgcgg
      421 gcgttcaaaa cgctccatcc ggacccgctg ctcgcgctgc gggcgctcga agcgctcgat
      481 gcgctgggcc cgctcgatcc gctcgggccg ctcgatgcgc acgcgctcct ggtgctgcgg
      541 ccgctcggcg cggacgcgct gctcgccgcc gccttcggcc ttgcgggcct cggcccgggc
      601 ctgagccttc tcggcccgct cctcggtcct ggcctggcgc tcgtcgtccg ggcgcgcctg
      661 cgccggtgca gtgattgcaa gcgccgcgac ggtcgcgatc aagaacattc tggtcatcgg
      721 ttaaacgctt gccggtgtga ggaatttcga tccgcattgg acgatatgcg gtgattgcag
      781 catgaacggc gccggcccgg gtcggccgtg cgcgccattc gccccgcccc cgcctcgatt
      841 cagcgcgcgg taatctggcg ttcaggaaaa ccgggcattg tcccacctcg ggcggtttgc
      901 gcacctgtcc agccgaaccg ggtgaaaccg gcccgtgctc cattcgttcg accgggcatg
      961 agttttgagg agcctgagca atgacacgca atatcctgat gacggcgctg gccacggcgg
     1021 cgatcgccgc ctccgctcct gcgctcgccc agccgggcgg cggtggcggg cccggcggcg
     1081 gagcgggcgc gggcccgccg atgactcccc ccggccagca gggcggcggt ccgggcaccg
     1141 cggattatgc ccgcgacaat atcgccgcgc agcagggtca gttcggccgc gacttcgccg
     1201 cccagcgcca gctgaccgcc gagcagcacc gggctctcgc cgcgcagcgg cgggcggatg
     1261 cgctggcctt ggcccaggcg gctcgcagcg gcgcccggat tccggccaat gccgccgggc
     1321 ggatccgcga ggcgctcaag gaagacatcg ctgcctggcg cgaggagttc gacgtcggcc
     1381 ggcgcgagtg gcaggcgatg cgcgaccagt ggatcgtcga ccgcagttcg ctgaccgccg
     1441 agcagtgggc cgagcgccgc gccgcctggt tcgccgcccg cgacgcgtgg atcgcgagcc
     1501 agaaggcctg ggcgtccgcc cgccgcaact aggacggctg caaacgacca cttgtcgaag
     1561 gaccgggcct gcgagggctc ggtccttcct cgcttgggcg tcaggccgcc cggtattcga
     1621 ggacgctgat cccggcgggc gtgtcggtca cgccgaccgg ccggaacccg cttcgctcgg
     1681 cgagccggtc atattcctgc cggctgcgct gccgggcggc gaacaaagcc atcatgtgga
     1741 cgtcgagcat cttcacccaa tgcgggcccg gggtcggcgg catcaattgc tcgatcagca
     1801 gcagcgccga tcccgtcgga gcggcccggc ggacggccgc aaggatttgc accgactcct
     1861 cgtcggtcca gtcgtggatc acctccatca atatgtaggc gtcgctttgc ggaagctcgt
     1921 cggcgaagaa atccccggcc tgaagctgca gccggtcgga ggcgattgcg cgcacctctt
     1981 cgatgacgtg cggctgctcg aacagcagcc ccctcgcctg gggatatctg gccaggatcg
     2041 cctgcagcaa atggccgcgc ccgccgccga cgtcggcgat ccttggaaag cgcgagaaat
     2101 cgaagctctc gaccacgccg gcgacctggc cgaacgactt gccgatcatc gccccgttga
     2161 agatcgcgct cgcttccggc cgcttggcaa gccacgccca cagcccgtcg ggatgagcct
     2221 tttccaccgc cggccggccg gtccggaccg aatggtcgag atctccgaac gtctcccaga
     2281 agaagggcag cccgaacatg cgggcaaggt cgcgggccga ctgcggatga tcggtgcgaa
     2341 gggtctccga gaccgggttg tgtgacaccg tgccgccgtc ggcggaaaag atgccgtggg
     2401 cgctgagcag gcgcaggatc cgacccagcg cgtcgggatc ggcgccggtc ttcgcggcca
     2461 gctcatcgac ggaagcgccg ccatcgccaa tgtggtccgc gacgcccaaa tccgccgcga
     2521 catgcagcgc ccgggcgaca caatagccgc cagtggcctg caggatcacg tccagcggcg
     2581 agcttgagtc ggttttcatc ggctcactcc cattcgatcg tcccgggggg cttcgacgta
     2641 tagtcataga ccacccggtt gatgcccggc acctcgttga cgatcctcgt cgcgacccgc
     2701 gccaggaacg cggcgtcgag cgggtagatg tcggcggtca tgccgtcgac gctggtcacc
     2761 gcgcgcaagc cgcagacatg gtcgtaggtg cgatggtcgc ccatcacgcc gacggtgcgc
     2821 accggcagaa gcaccgcgaa cgcctgccag atcgcgtcgt agaggccggc gttgcggatt
     2881 tcctcgaggt agatggcgtc ggccttgcgc aagatgtcgc agcgctcctc ggtgacctcg
     2941 ccggggatcc ggatcgccag ccccggaccg gggaacgggt ggcgctcgac gaagccctcg
     3001 ggcagcccga gctcgcgacc gagcacccgc acctcgtcct tgaacagttc gcgcaacggc
     3061 tcgaccaatt tcatcgccat gcgctcgggc aggccgccga cattgtggtg gctcttgatg
     3121 gtcacgctcg gcccgccggt gaagctgacg ctttcgatca catccgggta gagcgtcccc
     3181 tgggcgagga aatcggcgcc gccggccttg gccgcctcgg cctcgaaaat gtcgatgaag
     3241 gtcttgccga tgaacttgcg cttggcttcg gggtcggtga ccccggccag cccgccgagg
     3301 aagtccgcgc tcgcgtcgac gtggaccagg gggatgttgt agctgccccg gaacagcgag
     3361 acgacctgct cggcctcgcc caggcgcatc aggccatggt cgacgaacac gcaggtcagt
     3421 tgctccccga ccgcctcgtg gatcagcacc gcggcgaccg cgctgtcgac cccgcccgac
     3481 agcccgcaga tcacccggcc ctgcccgacc tgttcgcgga tctcggcgat cttggcgtcg
     3541 cggaacgccc ccatcgtcca gtcgccggcg cagccgcaca cgtgccggac gaaattggcc
     3601 agcagcctgc cgccgtcggg cgtgtgcacg acctcggggt ggaacatcat cgagtagaag
     3661 cggcggtcct cgtcggtggc gatcgcgtaa ggcgcgccct cggacttcgc gacgatcttg
     3721 aagccgtcgg gcagccgctc gacgcggtcg ccgtggctca tccacacctg gtgcgtctcg
     3781 ccgaccttcc acagcccgtc gaacaaggcc gattcatcga tgatctcgat gaacgcgcgg
     3841 ccgaactcgc ggttgtcgga cgccgcgacc accccgccga gctgatgggc catggtctgc
     3901 tggccgtagc agatggcgag gatcggcagc ccgcagtcga agaaatgctg cggcgcgcgg
     3961 gggctgtcgt cccacatcac cgacgacggc ccgcccgaca agatgatccc tcgcggcttg
     4021 aggcgctcga acgcctcgtc cgccgcgttg aacggggcga tttccgaata gaccccggcc
     4081 tcgcgcaccc gccgcgcgat cagctgggtc acttggctgc cgaagtcgac gatcaggatg
     4141 gattcggcgg gctggacggt catggcaggc cgatagccct tctgcgccac gagtaaagtt
     4201 cgaggaagcc gcccgcggca gcgacccgct gttgcgctgc ggccacgccc ggccggaatg
     4261 atggccccga aacggatgaa cccgcggcgg cgccccgttc cccggctgaa agggagggga
     4321 atccgatggc ctattaccgg ctgtatttcc tcggcggcgg aagcgggccg atcttcgact
     4381 tccgcgactt cgaggtggcc gacgacgatt ccgccatcgc cgtcgcggaa gggcttcgcc
     4441 gaatcggcgc gatggagctg tggtgcggcg gccgcagggt caggcgctgg gagccggttg
     4501 cggctgcgcg gcaagcgccg cctccgcttc ccgccgctcg aactgctcgg cgagcttgaa
     4561 cttctggatc ttgcccgagc cggtcagcgg ccactgatcg acccagatcc agtgctgcgg
     4621 ggtcttttgg ggggacagcc gctcgcggat gaacgccttc agttcggccg gctccgggcg
     4681 gcgggcgccg gcggcgcgca tgaagcaggc gacctgctcg ccataggtcg ggcacggcac
     4741 gccgaccacc gcgacctcgc cgatgtccgg gtgctcgagc atcgcattct cgatctcgac
     4801 cgggaacaga ttctcgccgc cgcggatgat catttccttc acccgcccgg tgatcgcgac
     4861 atagccgcgt tcgtccatcc gcccgaggtc gccggtgtgc agccagccgt cggcctcgat
     4921 cgccttggcg gtcgcctcgg gattgtcgtt gtagccggtc atgacgtgat agccccgcgt
     4981 gcagatctcg ccctgctcgc cgaccggaag caccttgcc
//
LOCUS       DASTZO010000119         2008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_218545,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000119 DASTZO010000000
VERSION     DASTZO010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218545"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VFU20_03330"
     CDS             <1..852
                     /locus_tag="VFU20_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007407128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU5285529.1"
                     /translation="TQDQAPWVFWTLTGLAFAIGFLLIIPIGGADMPVVISMLNSYSG
                     WAAAAMGFTLHNSAMIITGALVGSSGAILSYIMCRAMNRSFVSVIAGGFGGEALVAGG
                     GGAIDRPYKRGSAEDAAFLLKQAEQVIIVPGYGMAVAQAQHALREMGDLLKAEGVRIK
                     YAIHPVAGRMPGHMNVLLAEASVPYDEVFELEDINSEFAQTDVAFVIGANDVTNPAAK
                     TDKSSPIYGMPVLDVEKARTVLFVKRSMGGVGYAGVDNELFYRDNTMMLLADAKKMVE
                     EIVKNLG"
     gene            944..1660
                     /locus_tag="VFU20_03335"
     CDS             944..1660
                     /locus_tag="VFU20_03335"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HEU5285530.1"
                     /translation="MRKLGIVGGTSWSSTALYYEHINRGVAQRLGALHSARLAIESLD
                     LAPVAAMELSGDWDGVAAVLTEAAKRLKASGADGLLLASNTSHKVYDQVKAAVRLPML
                     HIADATAERLVADKRSRVALIGTRFTMTEGHIRDRLEARGITLAPVRPEWVREVDRII
                     FDELAAGRVVRDSQRKLKTLITDLAKQKVQAVVLGCTELVLAVDVRANVIPVYDTTAI
                     HARAAVDWMLDDEEQARAAA"
     gene            1702..>2008
                     /locus_tag="VFU20_03340"
     CDS             1702..>2008
                     /locus_tag="VFU20_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008993839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU5285531.1"
                     /translation="MDRTPADRPDHPQAAERFNEDKATYAVKGAGAPDLEAGVAAIRN
                     VLKTLPVRPGVYRMQDSRGDVLYVGKARTLKSRVTSYTQVARLPKRLQRMVSQTHSMT
                     "
BASE COUNT          341 a          672 c          708 g          287 t
ORIGIN      
        1 acccaggacc aggccccgtg ggtcttctgg accctgaccg ggctcgcctt cgccatcggc
       61 ttccttttga tcatcccgat cggcggcgcc gacatgccgg tcgtgatatc gatgctcaac
      121 agctactcgg gctgggcggc cgccgcgatg ggattcacgc tccacaacag cgcgatgatc
      181 atcaccgggg cgctggtcgg aagctcgggc gcgatcctgt cctacatcat gtgccgggcg
      241 atgaaccgca gcttcgtctc ggtgatcgcc ggcggcttcg gcggcgaggc gctggtcgcc
      301 ggcggtggcg gcgcgatcga ccggccgtac aagcgcggct cggccgagga cgcggcgttc
      361 ctcctgaagc aggccgagca ggtgatcatc gtcccgggct acgggatggc ggtcgcccag
      421 gcccagcacg cgctgcgcga gatgggcgac ctgctcaagg ccgagggggt gcggatcaaa
      481 tatgcgatcc acccggtcgc cggccggatg ccggggcaca tgaacgtgct gctggccgaa
      541 gccagcgtgc cgtacgacga ggtgttcgag ctcgaggaca tcaactccga gttcgcccag
      601 accgacgtcg ccttcgtcat cggcgccaac gacgtcacca acccggccgc caagaccgac
      661 aagagctcgc cgatctacgg aatgccggtg ctcgacgtgg aaaaggcgcg caccgtcctg
      721 ttcgtcaaac ggtcgatggg cggggtcggc tatgcggggg tcgacaacga gcttttttac
      781 cgcgacaaca cgatgatgct cctcgccgac gccaagaaga tggtcgagga gatcgtgaag
      841 aacctcggct gagccgaccc gaggggattc gcgggcattg catctgggtt ggagccccca
      901 gccggtcgcc ggttggggcg tggccggaga aggggaagcg gatttgcgca agctgggcat
      961 cgtcgggggc acgagctggt cctccaccgc gctctactac gagcatatca accgcggcgt
     1021 cgcgcagcgg ctcggtgcgc tgcacagcgc ccggctggcg atcgagagcc tcgacctggc
     1081 gccggtcgcc gcgatggagc tgtcgggcga ctgggacggg gtcgccgcgg tgctgaccga
     1141 ggcggcgaag agactgaagg cgtcgggcgc cgacggcctg ctgctggcgt ccaacacctc
     1201 gcacaaggtc tacgaccagg tgaaggcggc ggtgcggctg ccgatgctgc acatcgccga
     1261 cgcgacggcc gagcggctgg tcgccgacaa gcgcagccgg gtggcgctga tcggcacccg
     1321 cttcacgatg acggaaggcc acatccgcga ccgcctggaa gcgcggggga tcaccttggc
     1381 gccggtgcgg cccgagtggg tgcgcgaggt cgaccggatc attttcgacg aactcgccgc
     1441 cggccgggtc gtccgcgaca gccagcgcaa gctgaagacg ctgatcaccg atctcgccaa
     1501 gcagaaggtc caggcggtgg tgctcggctg caccgagctg gtgctcgcgg tcgacgtccg
     1561 cgccaacgtc atcccggtct acgacacgac cgcgatccac gcccgcgccg cggtcgactg
     1621 gatgctcgac gacgaggagc aggcgcgggc ggcggcctag cgcatctggc atccgcaccg
     1681 ggcgcggcct aaatcccacg catggaccgg acccccgccg accgacccga tcacccgcaa
     1741 gcggccgagc gcttcaacga ggacaaggcg acctatgcgg tcaagggcgc cggcgcgccc
     1801 gacctcgagg cgggcgtcgc ggcgatccgc aacgttctga aaacccttcc ggtgcggccg
     1861 ggcgtgtacc ggatgcagga ttcgcgcggc gacgtgctct atgtcggcaa ggcgcggacc
     1921 ctcaaaagcc gggtgaccag ctacacccag gtcgcgcggc tgcccaaaag gcttcagcgc
     1981 atggtgtcgc agacccactc gatgacca
//
LOCUS       DASTZO010000120         2412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_715004,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000120 DASTZO010000000
VERSION     DASTZO010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2412
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715004"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="VFU20_03345"
     CDS             complement(<1..500)
                     /locus_tag="VFU20_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5285532.1"
                     /translation="MRDIDHFIGGSSFASDDRPESRSAEVFDPNQGAVQARVRLGSAA
                     DLERAMESARAAQPAWAATNPQRRARVMFAFKALVEAHMDELAEILSSEHGKVIADSK
                     GDIQRGLEVIEFACGIPHALKGEYTVGAGPGIDVYSMRQPLGIVAGITPFNFPAMIPM
                     WMFGVA"
     gene            604..834
                     /locus_tag="VFU20_03350"
     CDS             604..834
                     /locus_tag="VFU20_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285533.1"
                     /translation="MRTAALLFCLLLVGAAPPAPDARLRSIAEPVSGAQMKRTIEKLV
                     SFGTRHTLSSQSDPKRGIGAASARRCAGPWPQ"
     gene            831..1997
                     /locus_tag="VFU20_03355"
     CDS             831..1997
                     /locus_tag="VFU20_03355"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5285534.1"
                     /translation="MKKTGLATMQVCDTVSGRRIPAPTRVCNAVAIQRGTTRPNDVVI
                     ITGHIDSRVTDPMNSTADAPGANDDGSGTAAVIEAARVLSKHKFPGTIVYAALSGEEQ
                     GLHGGKIMADYAKKQGWNVIATLNNDIIGNSCGSDGVCDDKHVRVFSEGPRWQGGDAL
                     RPQIRSLGGENDAPSRNLSRWLAGLAERLQIGLAVRQIWRNDRHGRGGDHTEFLNAGF
                     PAVRFSVAVENYNQQHQDVRVEKGVEYGDTIDKMDFPYLEKVTRLNVAALAALASAPP
                     PPEPTVEGAVSTDTTVSWPLVANATHVVRWRRTDSALWEFSRPHKCPVGVTTSRPQST
                     QHFDRCEVVLKGIRVDDWVFGVSSVGKDGFESPVASAVPGGAFKPWVAPSNPSP"
     gene            2049..>2412
                     /locus_tag="VFU20_03360"
     CDS             2049..>2412
                     /locus_tag="VFU20_03360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEU5285535.1"
                     /translation="MPRKRSPGLPSRKQILDFIATSDQPAGKREIARAFGLSGQDKVA
                     LKTLLKDMADEGLIDAAPGRAFHKSGGVPRVTVLKIVEADDSGNVWGVPEQWQAETPP
                     PKLKILERGRRGALGIGDR"
BASE COUNT          415 a          799 c          846 g          352 t
ORIGIN      
        1 atggcgaccc cgaacatcca catcgggatc atcgccggga aattgaacgg agtgatgccg
       61 gctacgattc caagcggctg gcgcatcgaa tagacgtcga tgccgggccc ggcgccgacc
      121 gtgtactcgc ccttcagcgc atgcgggatg ccgcaggcga actcgatcac ctccagcccg
      181 cgctggatgt cgcccttgga gtcggcgatc accttgccgt gctcggacga caggatttcg
      241 gcgagctcgt ccatgtgcgc ctcgaccagg gccttgaagg cgaacatcac ccgggcgcgg
      301 cgctgcgggt tggtcgcggc ccaggccggc tgggcggccc gcgccgactc catcgcccgc
      361 tcgaggtcgg cggcgctgcc gagccggacg cgcgcctgga cggcaccctg gttggggtcg
      421 aacacctcgg cgcttcggct ttcgggccgg tcgtccgacg cgaaggacga gccgccgatg
      481 aaatggtcga tgtcacgcaa cattggaaag cgtcctcaaa ttgggtccgg ggatcgccgc
      541 tccactagac ccgggcggcg gcaaatgcca gttggaagcc ggggcgggga gaggagaatt
      601 gcgatgcgaa ccgccgcctt gctgttctgc ctgctgctgg tcggcgccgc tccgccggcg
      661 cccgacgcgc ggctgcggtc gatcgcggag ccggtcagcg gcgcccagat gaagcgcacg
      721 atcgagaaac tggtgagctt tggaacccgc cacacgctgt cgtcgcagtc cgaccccaag
      781 cgcggcatcg gcgcggcatc ggcgcggcgg tgcgctgggc cgtggccgca atgaagaaga
      841 ccggccttgc gacgatgcag gtgtgcgaca cggtgagcgg ccggcggatc ccggcgccga
      901 cgcgggtctg caacgccgtc gcgatccagc gcggcacgac aaggcccaac gacgtggtga
      961 tcatcaccgg ccatatcgac agccgggtga ccgacccgat gaactcgacc gccgacgcgc
     1021 ccggcgccaa cgacgacgga tcggggaccg ccgcggtgat cgaggcggcg cgggtgctct
     1081 cgaagcacaa attccccggc accatcgtct acgcagccct gtcgggcgag gagcaggggc
     1141 tccacggcgg caagatcatg gccgattacg ccaagaagca gggttggaac gtgatcgcca
     1201 ctttgaacaa cgacatcatc ggcaacagct gcggctccga cggggtttgc gacgacaagc
     1261 atgtccgagt gttctcggaa ggcccgcgct ggcagggcgg cgacgcgctc cggccgcaga
     1321 ttcgaagcct cggcggcgag aacgacgccc cgtcgcgcaa cctgtcgcgc tggctcgccg
     1381 gactcgccga gcggctgcag atcggtctcg ccgtccgcca aatctggcgc aacgaccgcc
     1441 acgggcgcgg cggcgaccac accgagttcc tcaacgccgg tttccctgcg gtccgcttct
     1501 cggtcgcggt cgagaattac aaccagcagc accaggacgt gcgggtcgag aagggcgtcg
     1561 aatatggcga caccatcgac aagatggatt tcccgtacct ggagaaggtg accaggctga
     1621 acgtcgccgc tctggccgcg ctcgcctccg caccgccgcc gcccgagccg accgtcgaag
     1681 gcgcggtgag cacggacacg acggtaagct ggccattggt tgcaaacgcg acccatgtcg
     1741 tgcgctggcg gcggaccgat tctgcgctgt gggagttcag ccggcctcac aaatgcccgg
     1801 tgggagtcac gacttcccgt ccgcaatcaa cgcagcattt cgatcgttgc gaggttgttc
     1861 tgaagggcat ccgggtcgac gactgggtgt tcggcgtgtc gtcggtcggc aaggacggct
     1921 tcgagagccc ggtcgcctcg gcggttccgg gcggcgcgtt caagccctgg gtggcgccgt
     1981 ccaacccgtc accctgagtt tccgcaacgg atgctgaaac aagttcggca tgacggagta
     2041 gtgtagccat gccccgcaag cgctctcccg gactgccgtc gaggaagcag atcctcgact
     2101 tcatcgcgac gtcggaccag ccggccggca agcgcgagat cgcccgggcg ttcgggctgt
     2161 cgggccagga caaggtcgcg ctcaagacgc tcttgaagga catggccgac gaggggctga
     2221 tcgacgccgc gcctggccgc gcgttccaca agtcgggcgg ggtcccgcgg gtgacggtgc
     2281 tcaagatcgt cgaggccgac gacagcggca atgtctgggg cgtgcccgag cagtggcagg
     2341 ccgagactcc cccgccgaag ctgaaaatcc tggagcgcgg gcggagggga gcgctcggca
     2401 tcggcgaccg gg
//
LOCUS       DASTZO010000121        20304 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1107106,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000121 DASTZO010000000
VERSION     DASTZO010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 20304)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20304)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20304
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107106"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            745..1194
                     /locus_tag="VFU20_03365"
     CDS             745..1194
                     /locus_tag="VFU20_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285536.1"
                     /translation="MAEVSLFRLYLMRVVYLLIFVAVGTMALPPLLNQPEPITPMRGV
                     ALSFWMALSLLALVGVRYPLRMIPVLLIQMLYKIIWLLALAWPLWRSGTAFDPDMAEF
                     TRAMAIGIVVDVIAIPWGYVLANYVRRPGDRWTERGREPPVGEVDRH"
     gene            complement(1983..2489)
                     /locus_tag="VFU20_03370"
     CDS             complement(1983..2489)
                     /locus_tag="VFU20_03370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285537.1"
                     /translation="MAQTKNKRAKANIKRTKGQSATISRSISPFDLQYTLEPSGSAFV
                     FQFNIPKFPRDFKSSSISERDLLSKDYLRKYSLTTARDTGDISRQLEVLDVKPGVPVV
                     RARLPQVIDNVTVIMGDVFQNREAAINFLENEVSPTSGKNWIDAIAAGEIATFLVSLN
                     EMKYGSRG"
     gene            complement(2489..2977)
                     /locus_tag="VFU20_03375"
     CDS             complement(2489..2977)
                     /locus_tag="VFU20_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285538.1"
                     /translation="MTDTSHEIDTQVEASALPFGSNERDWVLAHVVRLTNVFGIGFEL
                     VLYTPTGVIAGKTISGQEYFDRIAKSFGDPSSKKAKDRLAGLFDSMGQQYKPDKEDGT
                     YSEDNPIQTKIPQYIHLDNCKIFYPGHSPFPDNDGILWRGNLDKVVGFTFGSVSIEKK
                     DA"
     gene            complement(3054..5081)
                     /gene="rpoD"
                     /locus_tag="VFU20_03380"
     CDS             complement(3054..5081)
                     /gene="rpoD"
                     /locus_tag="VFU20_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEU5285539.1"
                     /translation="MAKNETSEAEVVEGGDAPLIDLNDASIKKLIARAKKRGVITYDE
                     LNEALPQDQMSSEQIEDVMSAISEMGVNIVETAEDAEEEDEPEPAEEEIDPLDDGGPR
                     PAAATKKEAVDRTDDPVRMYLREMGAVELLSREGEIAIAKRIEAGRDTMIWGLCESPI
                     TFNAIIEWSNALNDGRMQLREILDLEAMLSKGPTPEQVAGSEEGEEGEISAAAAGPTF
                     KEEEEPEEEPAQVDEDEESLIERRAPRPADDDDEDNTLSLAQMEEQLKPTALEKFSTI
                     TSLYKKFAKLQAARLEALGAGHEYPPGDEKKYQKLREQLTAEVESVQFHASKIEYLVD
                     QLYSYNRRLTALGGQMLRLAERHRVPRRAFLDSYVGSELDDSWLARAAKIDKKFAAFA
                     IAEADTVERIRGEIGEIAQATGMALAEFRRIVNQVQKGEREARIAKKEMVEANLRLVI
                     SIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQ
                     ARTIRIPVHMIETINKLVRTSRQFLHESGREPTPEELAERLSMPLEKVRKVMKIAKEP
                     ISLETPIGDEEDSHLGDFIEDKNAVIPVDAAIQSNLKETVTRVLASLTPREERVLRMR
                     FGIGMNTDHTLEEVGQQFSVTRERIRQIEAKALRKLKHPSRSRKMRSFLDQ"
     gene            complement(5109..6956)
                     /gene="dnaG"
                     /locus_tag="VFU20_03385"
     CDS             complement(5109..6956)
                     /gene="dnaG"
                     /locus_tag="VFU20_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU5285540.1"
                     /translation="MSLSVQFLDELRARTLLSAVIAPSVKLLKAGREYKACCPFHQEK
                     TPSFTVNDEKGFYHCFGCGAHGDAIRFLTDSRGMPFMDAVKELAGRAGMEVPAPDPRA
                     RDQAERAAGLTDVMAAAQKWFADQLQGSEGAEARDYLEGRGIDAATAARFGIGLAPQG
                     RTRLRQALGHLGEDKLVETGMLIKPEEGGGESYDRFRGRLMFPIRDQRGRVIAFGGRI
                     LGDGEPKYLNSPDTILFDKGRTLYNIDRAAPASRSAKRLVVVEGYMDVIALDRAGISE
                     VVAPNGTAVTESQLERMWRLDPAPILCFDGDSAGRKAAVRAALRALPHIGPDRTLRFV
                     ELPPGQDPDDVVRSGGKEAFEALLARPEPLDERLWRHERDSAPLATPEARAGLRQRLI
                     EHSQAIGEPALRRLYRDQWLNRFDSEIAPQRPPRGAPNRPFRPFRPGSRYTPPDPPAS
                     DTARAIGAGGIDRATARALIVGFANHPEALAAHCEQLAALPIADPRSARLRDHLVNAA
                     FSSAALDRESLATILENAGAAADLDAARSTGGIGFSFTRSDAEPAKAVRDLGVAIEAL
                     SADGEIGAALAEATERLKAGEEQAFEEQRRLHVAREKVKERLASLTGTD"
     gene            7208..7588
                     /locus_tag="VFU20_03390"
     CDS             7208..7588
                     /locus_tag="VFU20_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285541.1"
                     /translation="MLTKISGLARALYILLAIVTAFVALGALDVALTLVVLGLIAGIT
                     MPRDRMVLAAATVIALPYIGTALGNIPAIGAQLTAFAGNLQLGVAGALASALAILLYQ
                     LVIEGVTGLIGGSATGKPATATAG"
     gene            complement(7658..8110)
                     /locus_tag="VFU20_03395"
     CDS             complement(7658..8110)
                     /locus_tag="VFU20_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEU5285542.1"
                     /translation="MIRDDIKAALVAAMKSGDKAATGTLRMVSSAIKNRDIEARTGPA
                     SADDDAMVTEVLQKMIKQRRESADLYRKGNREELAQAEEAEIAVIERFLPRQLSDEEA
                     RAAIAAIVAETGAGSMKDMGKVMALVKQRHATAIDPARASGIVREALS"
     gene            8275..9468
                     /gene="carA"
                     /locus_tag="VFU20_03400"
     CDS             8275..9468
                     /gene="carA"
                     /locus_tag="VFU20_03400"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU5285543.1"
                     /translation="MADARPSPLAAPAPQPAPRPEGATGVVVFADGRTVWGQGFGAEG
                     EAVGELCFNTAMTGYQEVLTDPSYAKQVIAFTFPHIGNVGANDEDVEAAEAHALGCLV
                     REVVTRPSNYRAGEDFATWMRKWGRIGIAGVDTRALTRLVRREGPPTIAIAHWSDGKF
                     ELDVLQRMAADWPGLEGMDLARDVSRLQVERWTGGKWAWGQGYAMGGDDAKRPHVVAI
                     DYGSKRNIFRNLVEAGARVTIVPAEASFDEVMAHEPDGIFLSNGPGDPAATADYAVPV
                     IRRLLETKKPLFGICLGHQLLALAVGGKTAKMFQGHRGANHPVKRLADGRVEITSMNH
                     GFAVERDGLPANARETHVSLFDGSNCGFELTDRPAFSVQYHPEASPGPQDSSYLFEKF
                     VGAMR"
     gene            9465..9734
                     /locus_tag="VFU20_03405"
     CDS             9465..9734
                     /locus_tag="VFU20_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285544.1"
                     /translation="MRAAALASIALLLAGCVTARLHSEEELATVGRDCGLALGELFQD
                     ESEKKLVFLFKPGASREQRACVVRWARKNHLKTVIVEAINFPEES"
     gene            9739..13098
                     /gene="carB"
                     /locus_tag="VFU20_03410"
     CDS             9739..13098
                     /gene="carB"
                     /locus_tag="VFU20_03410"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU5285545.1"
                     /translation="MPRRTDIASILIIGAGPIIIGQAAEFDYSGSQALKTLKAEGYRV
                     IVVNSNPATIMTDPELADATYIEPITPGIVAKILEKERPRHPGGFALLPTMGGQTALN
                     TALALARDGTLERLGVELIGANAAAIEMAEDRLKFREAMDRIRLESPRSAIAHSLDDA
                     LAALDSVGLPAIIRPSFTLGGTGGGIAYNREEFEAIVRGGLEASPSSEVLIEESVLGW
                     KEYEMEVVRDRADNAIIICSIENVDPMGVHTGDSITVAPALTLTDKEYQRMRSASIAV
                     LREIGVETGGSNVQFAIDPKTGRMVVIEMNPRVSRSSALASKATGFPIAKVAARLAVG
                     YTLDEIANDITGGATPASFEPTIDYVVTKVPRFAFEKFRGAEATLSTSMKSVGEVMAI
                     GRCFAESLQKALRGLETGLTGLDRVRELEHSDPAAIESALARPTPDRLLVAAEALRQG
                     FTVERINAIAGFDPWFLERLLEIVEAEEQVRSEGLPQDEAGMRELKAMGFSDARLAEL
                     ALRSAKAPRGMSEKSARGSGVVHDALKALTGGVTEDEVRAHRLKLGVRPVFKRIDSCA
                     AEFDATTPYLYSTYEAPLFGEAEDEAEVSDKRKVIILGGGPNRIGQGIEFDYCCCHAA
                     FALGREGAGLETIMVNCNPETVSTDPDTSDRLYFEPLTAEDVIEIVERESAKGELLGV
                     IVQLGGQTPLKLAAELQAAGVTILGTSPDSIDLAEDRERFARLVARLGLLQPPNGIAR
                     SRDEALQVAQRIGYPVLLRPSYVLGGRGMEVVDGPAQLDHYIATAVAVSGSSPVLIDR
                     YLRDAIEVDVDAICDGKEVAVAGVLQHIEEAGVHSGDSACAIPPHGLSSEVVAEIERQ
                     TRALALALDVRGLMNVQFAVKEEQVYLIEVNPRASRTVPFVAKAIGRPIAKIAARVMA
                     GEPLSAFDPIDRDIGHIAVKESVFPFARFPGTDPVLGPEMRSTGEVMGIDRDFDIAFA
                     KALLGAGMELPDGGTAFVSVKDADKDHIVPAVEKLVELGFAIIATGGTAGHLAERGLP
                     VTPVNKVAQGRPHIVDRLLDGDVDLVFNTTEGWQSLKDSHSIRATALARKVPYFTTAA
                     ASLAAARSIEAVRGHALEVASLQSYYSASVN"
     gene            13183..13662
                     /gene="greA"
                     /locus_tag="VFU20_03415"
     CDS             13183..13662
                     /gene="greA"
                     /locus_tag="VFU20_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEU5285546.1"
                     /translation="MATADKVPMLAEGHRKIDEEVRHLKTVERPAAIEAIEMARAHGD
                     LAENAEYHAAKERQGQIEATIADLEDRLSRAQVIDPTTLSGDKVVFGATVTLIDEDKK
                     KIRYQLVGQTEADAKVGRISYNSPLGRALIGRHAGEEVEVSTPSGDRYYKIKAIEFV"
     gene            13679..14305
                     /locus_tag="VFU20_03420"
     CDS             13679..14305
                     /locus_tag="VFU20_03420"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU5285547.1"
                     /translation="MRIPRTATIALAGVTALAWLVVAAIGADDHAAALWGFIPARLSG
                     LLDLPAAVPAALTPLSSTLVHGGLLHVAFNLLMLLWCGTAVERVLGRGALLLLYVVGA
                     YAAAVAQWLADPASIVPVIGASGAISAVIGAFALSFGEARRITRSPRLNRLLNAAWLL
                     AAWVVMQMMIGWLAGGRGLLLATPAHVGGFLAGLLLQRPLLLWRYRSA"
     gene            complement(14302..14550)
                     /locus_tag="VFU20_03425"
     CDS             complement(14302..14550)
                     /locus_tag="VFU20_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HEU5285548.1"
                     /translation="MSKLHLVFGGRVKDPRGLEFVDLDSIDLVGLYSSYAEAEDAWRG
                     KAQRTVDDAEMKYVVVHLHRLLEPDAESASAAPAAESA"
     gene            complement(14547..14966)
                     /locus_tag="VFU20_03430"
     CDS             complement(14547..14966)
                     /locus_tag="VFU20_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285549.1"
                     /translation="MTDQDKERQDRESGVRRRAIEAYDGARGRVAGAGRKATDAIDEA
                     PLLALAGGLAAGALIAALLPRTQSETQLLRPTADKLTGTARAAAEAAREAGTSRLAEL
                     GLTREKGAETLRSIFEGASDAAKTSAQAALDAARTRG"
     gene            complement(14963..15331)
                     /locus_tag="VFU20_03435"
     CDS             complement(14963..15331)
                     /locus_tag="VFU20_03435"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HEU5285550.1"
                     /translation="MSGDAPNVAAARIEAERARARLMETARELQERLSPGTLAQNAWA
                     GAKSKGADLAEEAVDAVRRRPAIAGGVVAALALFLAREPLIDMAGKLAGGATAKRKAR
                     KGKAAPRKTKSKAKKTETIE"
     gene            complement(15328..15693)
                     /locus_tag="VFU20_03440"
     CDS             complement(15328..15693)
                     /locus_tag="VFU20_03440"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU5285551.1"
                     /translation="MLKPNDPARQPPEPPEPGDERPIGELVHQLVEEGKAYAQAELGV
                     ARAIASAKADRLRVPAILLGAALLFAQAAVTVLAVAVFLGLLALMGPILAGLLAFLVF
                     AGGAAALAWFGIEKAKRDL"
     gene            complement(15693..16271)
                     /locus_tag="VFU20_03445"
     CDS             complement(15693..16271)
                     /locus_tag="VFU20_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285552.1"
                     /translation="MQDNERELPEGTDKVVSGASTPTSAEGEDGDVLLVADTGQPTAQ
                     PAGERTGRAATRDVIVDKIRTGREKLAGQAADKTRGLVGQGLERGAEALANVGKLVGD
                     TADGLDERLGPEYGDYARRAATAIEDAANNLAGKDPDELIDDTREFVRKSPGVALAGA
                     AIVGFALARLIKSGLARDEDEDVSRSRGKRES"
     gene            16414..17703
                     /gene="eno"
                     /locus_tag="VFU20_03450"
     CDS             16414..17703
                     /gene="eno"
                     /locus_tag="VFU20_03450"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU5285553.1"
                     /translation="MPAIVDVHGRQILDSRGNPTVEVDVTLEDGSMGRAAVPSGASTG
                     AHEAVELRDRDDARWCGKGVEGAVRSVNGEIAEAIVGREAGDQADIDAALIALDGTAN
                     KSRLGANAILGVSLASAKAAAESLGLPLYRYVGGVGAELLPVPMMNILNGGAHADNAI
                     DFQEFMVMPVGAPSFSEALRCGAEIFHALKSALAAAGLSTAVGDEGGFAPAISSSREA
                     LDLIMRAVASAGYAPGDHVLIALDCAATEYFHDGGYRLEGEGRSFTPEAMTDYLAELA
                     DAYPIASIEDGMAEDDMAGWKQLTERLGGRVQLVGDDLFVTNEQRLASGIAGGIANSI
                     LVKVNQIGTLTETIAAVRLAQGSGYTAVMSHRSGETEDTTIADLAVALGCGQIKTGSL
                     ARSDRTAKYNQLLRIEEELGESARYAGRAALKAFVTR"
     gene            17774..18085
                     /locus_tag="VFU20_03455"
     CDS             17774..18085
                     /locus_tag="VFU20_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU5285554.1"
                     /translation="MGAAQQARGLIRRAAMPALALIVVGNFAGYAIAGPNGLLAWGGY
                     HRDLNERKAELARLEAERAQLRHRSALLDPKKADPDIADELVRRDLGLVRPDEVIIPL
                     E"
     gene            18181..19278
                     /gene="pdhA"
                     /locus_tag="VFU20_03460"
     CDS             18181..19278
                     /gene="pdhA"
                     /locus_tag="VFU20_03460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEU5285555.1"
                     /translation="MARAAKPKSKPAAAPPPANRAKAASPPPRKAPNRERPPEPKRHK
                     AERDELLGLYKQMLLIRRFEERAGQLYGLGLIGGFCHLYIGQEAVAVGLQSAMTVGKD
                     SVITGYRDHGHMLAYGIDPKLIMAELTGRAAGISKGKGGSMHMFSVEHGFYGGHGIVG
                     AQVSLGTGLGFKHRYAEDGGVCLTYFGDGAANQGQVYECFNMAQLWKLPVIYAIENNQ
                     YAMGTSVERSSSEPDFYKRGESFRIPGLPVDGMDVLAVRGAAETALEWTRSGKGPIIL
                     ELKTYRYRGHSMSDPAKYRTREEVQEFREHNDPIGNAARDLAKLGVGEDELKAIDKQI
                     KDIVVEAAKFAEDAPLPDGSELYTDVLVGSY"
     gene            19278..>20304
                     /locus_tag="VFU20_03465"
     CDS             19278..>20304
                     /locus_tag="VFU20_03465"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEU5285556.1"
                     /translation="MATELTMPALSPTMEEGTLAKWLVKEGDEVKSGDILAEIETDKA
                     TMEFEAVDEGTIAKILVPEGSDGVKVGTPIAVLTADGEDASALPAAPKHDETPQPTPS
                     DQKTEAAPIETEQPQKAETGTAQLAAAPAAPAGPEVPEGTALAPLSVREALRDAMAEE
                     MRRDERVFVLGEEVAEYQGAYKVTQGLLEEFGPRRVVDTPITEYGFAGLGTGAAMGGL
                     RPIVEFMTFNFAMQAIDHIVNSAAKTNYMSGGQMRCPIVFRGPNGAAARVAAQHSQNY
                     GAWYASVPGLVVIAPYSAADAKGLLKAAIRTDDPVVFLENELLYGQHFDVPQMEDYVL
                     PIGKARIV"
BASE COUNT         3234 a         6760 c         6878 g         3432 t
ORIGIN      
        1 gtcgcactgc aacgcgtgcg cgcgcgcgta cgcatgcgtg cgcgagggga tcggttggcg
       61 gggcgagggg gtcgggcgca ggctgaagcg aggctgcatc ggccagctta gggcataaaa
      121 cgggggctgt aggacagcgg attcgtgagg gggtggttgt ccaaggcgcc gtcacgggcg
      181 gccgtgacgt cgatcgggtt cggctctgcc gacccgtcgg cagcggccgt tccgtcatgc
      241 ccccggcatg acggccgtgc ggccgcttct cgcggtctat tcgcgctgct cgacaattgc
      301 cgtgcaattc tctcgctgcg ctctgccgct cgaaggaaga gtggaagccc tgcctgcagg
      361 ggaagcggag gggcagggct tccggtcgtc gttccggcag ggccgcccga ggggcaatca
      421 ggagcggctc tgctgagccc gggtttaccg cctcgcggtt agcggcgggt gagcggacat
      481 ggtttagggg aattaaggtt tgggcgcccg agcgcagcga cgccggcgcg aagcgtcgcc
      541 ggagcagcgc gaagagagcg aaaaatgccg gccggcgggg cgcgagcctg gagcgagcga
      601 acccgaccga cgtcacggga ttcactcccg tgacggcttt tgcctgcggc aataccgcgc
      661 ggatccgggg tctcagaaag ccgttccgga aatctccggc gcagagtaac ctggccgcct
      721 ggaatggccg aaggggaggg cgccatggcc gaggtttcgc tgtttcgatt gtatctgatg
      781 cgggtggtgt acctgctgat attcgtcgcc gttggaacca tggcgctccc gccgctgctc
      841 aatcagcccg aaccgatcac gccgatgcgc ggcgttgccc tgagcttctg gatggcgttg
      901 tcgctgctgg cgctggtcgg cgtccgctat ccgctgcgga tgatcccggt attgctgatc
      961 cagatgctct acaagatcat ctggctgctg gcgctcgcct ggccgctgtg gcgaagcgga
     1021 acggccttcg atcccgacat ggccgagttc acccgcgcga tggcgatcgg gatcgtcgtc
     1081 gacgtgatcg cgatcccgtg gggctatgtg ctcgctaatt acgtgcgccg gccgggggac
     1141 cggtggacgg aacgcggtcg ggagccgcca gtgggcgagg tcgaccgcca ctgagcaccg
     1201 gcgccctacc tggcggcaat cgggagccag cgcgcagaga gggaaaaccc tccggcgggg
     1261 ccgcgagcgt ggagcgagcg aattcgtaat ctgtgcacgg tcaccgtaaa catcagcagg
     1321 tcaattcacc gttggggagt tgaacgccgc tatacgctaa acgataaata atcccgtggc
     1381 gggtgtcggg atcgcgcaga tcagcggaaa ataaaatcct ctcacaaatt tactttcggc
     1441 gcgaaatgaa gacgaaactc atcttctgga tcaagaacac caaatgtagc tgcgcctaac
     1501 ggacagtctt caacatacac agctaggttt cgcccaccgg catgctcagt ggacggaaaa
     1561 agaatggcag aacagttcat agacacggtg tagtctccgc accgccaacc cgcgaaatct
     1621 gagcttgcgt cacccgcctt caagctaccg tacgccgcct tccaatcgcc cgtaaggctt
     1681 tcttgccaat ctgaatatgt cgcggtaaga tcacctgtta aatccaccac gttgcaaatg
     1741 tccaaggtac caggcactaa tacgcagggc ctatccacct ttcctctgta gtattccgca
     1801 agagcggtgt ccggatcgag agaacagtac agagccgaca tgttttcacg attaaatctc
     1861 cctgcgttta ttctagagcc tttcgcgcta aacgcagact tgaaaaaggt tatcgatgtg
     1921 gcacggaaga cgtctaacct cgtgacagtc ccgtacaaga tgggcggcac gggaaccaaa
     1981 ggttatcctc tggaaccata tttcatctcg tttagcgaga ctagaaaggt cgcgatttca
     2041 ccagcagcga tcgcatcgat ccaattcttc ccagacgttg gagaaacttc gttctccaaa
     2101 aagtttatag ctgcctccct attttgaaac acatctccca tgattaccgt aacgttgtcg
     2161 atcacttgcg gtaatcgggc acgcaccacc ggtacaccgg gtttgacatc aaggacctcc
     2221 agctgacggg agatatcgcc tgtgtcgcga gcagttgtta acgagtattt gcgtaggtaa
     2281 tctttcgata gcagatctct ttcgcttatg gacgatgatt taaaatctct agggaacttc
     2341 ggaatgttaa actgaaacac aaacgcactc ccggaaggct ctaatgtgta ttgcagatcg
     2401 aaaggcgata tgcttcgaga tatcgtagca gattggcctt ttgtgcgttt gatgtttgct
     2461 ttggcgcgct tgtttttagt ttgagccatt aggcatcctt tttttcaatc gacacagagc
     2521 caaacgtgaa accaactact ttgtccaaat ttcctctcca cagaattccg tcattgtccg
     2581 gaaacggcga gtgtcctgga taaaatatct tacagttatc taaatgaata tactgaggta
     2641 tctttgtttg tattgggtta tcttctgaat aggttccatc ttctttatca ggcttgtact
     2701 gttggcccat actatcaaat agaccggcta gtctatcttt agccttttta gacgacggat
     2761 cgccaaacga tttcgcaata cgatcaaaat actcttgtcc tgatatcgtt ttgccggcaa
     2821 ttactcctgt aggcgtatac agaacgagtt caaagccgat accaaacacg ttcgtaaggc
     2881 gaacgacatg cgcgagtacc caatcgcgct cattagagcc gaacggcagt gcgctggcct
     2941 caacttgtgt atcgatttcg tgtgaagtgt cagtcatacg tctcctgaac ttagcgaacg
     3001 gcctagagac cagcggcatg ggaccttggt ccgggctttg aagttaaggc gcctcactga
     3061 tcgaggaacg accgcatctt ccttgaccta ctcggatgct tcaatttccg cagcgccttg
     3121 gcctcgatct ggcggatccg ctcgcgggtg acgctgaact gctggccgac ttcctcgagc
     3181 gtgtggtcgg tgttcatgcc gatgccgaag cgcatccgca gcacgcgctc ctcgcggggg
     3241 gttagcgagg cgaggactcg ggtgacggtt tccttcaggt tcgactggat cgccgcgtcg
     3301 accgggatga ccgcattctt gtcctcgatg aagtcgccga ggtggctgtc ctcctcgtcg
     3361 ccgatcgggg tctcgaggct gatcggctcc ttggcgatct tcatcacctt gcggaccttc
     3421 tcgagcggca tcgacaggcg ctcggcgagc tcctccgggg tcggctcgcg gccgctttcg
     3481 tggaggaact ggcggctggt gcggaccagc ttgttgatgg tctcgatcat gtgcaccggg
     3541 attcggatgg tccgcgcctg gtcggcgatg gagcgggtga tcgcctggcg aatccaccag
     3601 gtcgcgtagg tgctgaactt gtagccgcgg cggtactcga acttgtcgac cgccttcatc
     3661 agcccgatgt tgccttcctg gatcaggtcg aggaactgca ggccgcggtt ggtgtatttc
     3721 ttggcgatcg agatgacgag gcgcaggttg gcctcgacca tttccttctt ggcgatgcgg
     3781 gcctcgcgct cgcccttctg gacctggttg acgatgcggc ggaactcggc gagcgccatt
     3841 ccggtcgcct gggcgatctc gccgatctcg ccgcggatgc gctcgacggt gtcggcctcg
     3901 gcgatggcga aggcggcgaa cttcttgtcg atcttcgccg cgcgggcgag ccagctgtcg
     3961 tcgagctcgc tgccgacgta gctatcgagg aaggcgcggc gcgggacccg gtggcgctcg
     4021 gcgaggcgca gcatctggcc gcccaaggcg gtcagccggc ggttgtacga atagagctgg
     4081 tcgacgagat attcgatctt cgaggcgtgg aactggacgc tctcgacctc ggcggtcagc
     4141 tgctcgcgca gcttctggta ctttttctcg tcgccgggcg gatattcgtg gccggcgccg
     4201 agcgcctcga ggcgggcggc ctggagcttg gcgaacttct tgtagagcga ggtgatggtc
     4261 gagaatttct cgagcgcggt cggcttgagc tgctcctcca tctgggcgag cgacagggtg
     4321 ttgtcctcgt cgtcgtcgtc ggccgggcgc ggggcgcggc gctcgatcag gctctcctcg
     4381 tcctcgtcga cctgggccgg ctcctcctcg ggctcctcct cctccttgaa ggtcgggccg
     4441 gcggccgcgg cgctgatctc gccttcctcg ccttcctcgc tgccggcgac ctgctcgggg
     4501 gtcgggccct tggacagcat cgcctcgagg tcgagaatct cgcgcagctg cattcggccg
     4561 tcgttcaagg cgttcgacca ttcgatgatc gcgttgaagg tgatcgggct ttcgcacagc
     4621 ccccagatca tcgtgtcgcg gccggcctcg atgcgcttgg cgatggcgat ctcgccctcg
     4681 cggctgagca attccaccgc gcccatctcg cgcaggtaca tgcgtaccgg gtcgtcggtg
     4741 cggtcgacgg cttccttctt tgtcgccgcg gccgggcgcg ggccgccgtc gtcgagcggg
     4801 tcgatctcct cctcggcggg ctcgggctcg tcctcttcct cggcgtcctc agccgtctcg
     4861 acgatgttga cgcccatctc cgagatcgcc gacatcacgt cctcgatctg ctcggacgac
     4921 atctggtcct gcggcagggc ctcgttcagt tcgtcgtagg tgatcacccc gcgcttcttg
     4981 gcgcgggcga tcagcttctt gatcgacgcg tcgttgagat cgatcagcgg agcatcgccg
     5041 ccttcgacga cttcggcctc ggaagtttcg tttttcgcca tattccctaa ttttaccgcc
     5101 agcgccgctt agtccgtgcc ggtcagcgac gccagccgtt ccttgacctt ctctcgagcg
     5161 acgtgaagcc gcctttgttc ctcgaacgcc tgttcctcgc cggcctttaa acgctcggtc
     5221 gcctcggcaa gtgccgcccc gatctcgccg tcggccgaca gcgcctcgat cgccaccccg
     5281 agatcgcgca ccgccttggc gggttcggcg tcgctgcggg tgaaggaaaa tccgattccg
     5341 ccggtgctgc gcgcggcgtc caggtcggcg gcggctccgg cgttttccaa tatggtggcg
     5401 aggctctccc gatcaagcgc ggcgctggag aaagcggcgt tgaccaggtg atcgcgcagg
     5461 cgcgcgctgc gcggatcggc gatcggcagc gccgccagtt gctcgcaatg cgcggccagc
     5521 gcctcgggat ggttggcgaa gccgacgatc agggcgcgcg cggtcgcccg gtcgatgccg
     5581 ccggcgccga tcgcccgcgc ggtgtcgctc gccggcgggt cgggcggcgt gtagcgcgaa
     5641 ccggggcgaa aagggcggaa cgggcggtta ggggcgccgc gcggcggccg ctggggcgcg
     5701 atttccgaat cgaagcggtt aagccattgg tcgcggtaca gccgccggag cgcgggctcg
     5761 ccgatcgcct ggctgtgctc gatcaggcgc tggcgaagcc cggcgcgggc ctcgggcgtg
     5821 gccagcggcg ccgaatcgcg ctcgtggcgc cacagccgct cgtcgagcgg ctcgggcctg
     5881 gcgagcaatg cctcgaacgc ctcctttcca ccgcttcgga cgacgtcgtc ggggtcctgg
     5941 ccgggcggca gctcgacgaa gcgcagggtc cggtcggggc cgatgtgggg gagggcgcga
     6001 agggccgcgc ggaccgccgc cttgcggccg gcgctgtcgc cgtcgaagca caggatcggc
     6061 gcgggatcga gccgccacat ccgctcgagc tggctttccg tgaccgccgt cccgttgggc
     6121 gcgacgacct cggagatccc ggcgcggtcg agggcgatga cgtccatata gccctcgacg
     6181 acgaccagcc ttttcgccga gcggctggcg ggcgcggcgc ggtcgatgtt gtagagggtg
     6241 cggcccttgt cgaacaggat tgtgtccggc gagttcaggt acttgggctc gccgtctccc
     6301 agaatgcggc cgccgaacgc gatcacccgg ccgcgctggt cgcggatcgg gaacatcagc
     6361 cggccacgga accggtcgta gctctcgccg ccgccctcct cgggcttgat cagcatgccg
     6421 gtctcgacga gcttgtcctc gccgagatgg ccgagcgcct gcctcagccg ggtgcggccc
     6481 tggggcgcga ggccgatccc gaaccgggcg gcggtcgcgg cgtcgatccc gcggccctcg
     6541 agatagtcgc gcgcctcggc gccctcgctg ccctgcagct ggtcggcgaa ccatttctgc
     6601 gccgcggcca tgacgtcggt caggccggcg gcgcgctcgg cctggtcgcg ggcgcgcggg
     6661 tcgggggcgg ggacctccat gcccgcccgg ccggcgagct ccttgaccgc gtccatgaat
     6721 ggcatcccgc gcgaatcggt caggaagcgg atcgcgtcgc cgtgggcgcc gcagccgaaa
     6781 cagtggtaga agcccttctc gtcgttgacc gtgaagctcg gcgtcttctc ctggtggaac
     6841 gggcagcacg ccttgtactc gcggcccgcc ttcagcagct tcaccgacgg cgcgatcacc
     6901 gccgacagca aggtccgcgc gcgcagctcg tcgaggaact gaacggacag gctcacggga
     6961 tgagccttcc aagtgaagag ggggaaaggg tcacgaccgt agcgatactg cgtgggctgg
     7021 gggcgctcaa ccgagtcggc ccctaagccc caaataagct gggtgaagat ttaacaacgc
     7081 cggctgaagc ttgatttgaa aggattcatt tcacctgtga aaggagggga atggagctaa
     7141 tctgtgtccg ttccgcacct tggcggcaat ccaccgccga gacggagcaa aaccggaggg
     7201 ggattttatg ttgaccaaga tcagcgggct ggcccgcgca ctctacatct tgctggcgat
     7261 cgtcaccgca ttcgtcgccc tcggcgcgct ggacgtggcg ttgacgctgg tcgtactcgg
     7321 cctgatcgcc ggtatcacca tgcccaggga ccgaatggtc ctggctgccg cgacggtcat
     7381 cgcgctgccg tatatcggca ccgcgctggg caacatcccg gccatcggcg cacagctcac
     7441 cgcgtttgcc ggcaatcttc agctgggggt cgcgggggcc ttggcgagcg cccttgcaat
     7501 cttgctctat cagctcgtga tcgagggcgt gaccgggctc atcggcggca gcgcaaccgg
     7561 caagccggca accgcaaccg cgggatagtc atcattcctt gcctggcccc gccgcccgcc
     7621 gcggcggggc ctttttcctt gtcgcgaccg gcttcgctca ggacagcgcc tcccgcacga
     7681 tcccgctcgc ccgcgcgggg tcgatggcgg tggcgtggcg ctgcttcacc agcgccatca
     7741 ccttgcccat gtccttcatc gagccggccc cggtttcggc gacgatcgcg gcgatcgcgg
     7801 ctctggcttc ctcgtcgctc agctggcggg gcaggaagcg ctcgatcacc gcgatctccg
     7861 cttcctcggc ctgggcgagc tcctcgcgat tgcctttgcg atagaggtcg gccgactcgc
     7921 ggcgctgctt gatcattttc tgcaggacct cggtgaccat cgcgtcgtcg tccgcgctcg
     7981 ccgggccggt ccgcgcctcg atgtcgcggt tcttgatcgc cgaggaaacc atgcgcagcg
     8041 ttccggtcgc ggccttgtcg ccgctcttca tcgccgcgac caaggcggcc ttgatgtcgt
     8101 cgcgaatcat cgcatccctc ttatcgtcgc attcaagggc cgtatgggcg gatagacggt
     8161 ggtagcgccc gccgcggagc ttttacagcg ttgaccttcc gccccgcggc ccgtagcgac
     8221 catcgcttag cgacatcgca agcaaaaccg cccgcggaaa ggctctccag gcccatggcc
     8281 gatgcacgtc ccagcccctt ggccgccccc gcgcctcagc ccgcccctcg gccggagggc
     8341 gcgacgggag tggtggtgtt cgccgacggc cggacggtgt ggggacaagg cttcggggcc
     8401 gagggcgagg cggtcggcga gctgtgcttc aacaccgcca tgaccggcta ccaggaggtg
     8461 ttgaccgacc cctcctacgc caagcaggtg attgccttca ccttcccgca catcggcaat
     8521 gtcggcgcca acgacgagga cgtcgaggcg gccgaggccc acgcgctggg atgcctggtg
     8581 cgcgaggtgg tgacgaggcc gagcaactac cgcgccggcg aggatttcgc gacctggatg
     8641 cgaaaatggg gccggatcgg gatcgccggg gtcgacaccc gcgcgctgac ccggctggtg
     8701 cgccgcgagg gcccgccgac catcgccatc gcccactggt ccgacggcaa gttcgagctc
     8761 gatgtgctgc agcgcatggc ggccgactgg ccggggctcg aaggcatgga cctggcaagg
     8821 gacgtcagcc ggctgcaggt cgaacgctgg accggcggca aatgggcgtg ggggcagggc
     8881 tatgcgatgg gcggcgacga cgcgaagcgc ccgcacgtgg tcgcgatcga ctatggctcg
     8941 aagcgcaaca tcttccgcaa cctggtcgag gccggggcca gggtgaccat cgtccccgcc
     9001 gaggccagct tcgacgaggt catggcgcac gagcccgacg gcattttcct gtccaacggc
     9061 cccggcgacc cggcggccac cgccgattat gcggtgccgg tgatccgccg gttgctggag
     9121 acgaaaaagc cgctgttcgg gatctgcctc ggccaccaat tgctggcgct cgcggtcggc
     9181 gggaaaacgg cgaagatgtt ccagggccac cgcggcgcca accatccggt caagcggctc
     9241 gccgacggcc gggtcgagat cacatccatg aaccacggct tcgcggtcga gcgcgacggg
     9301 ctgccggcca acgcccgcga gacccacgtg tcgctgttcg acggcagcaa ttgcggcttc
     9361 gaattgaccg accgcccggc gttcagcgtc cagtaccatc ccgaagcgag ccccgggccg
     9421 caggacagct cctatttgtt cgagaaattc gtcggggcga tgcggtgagg gcggccgcgc
     9481 ttgcctcaat cgctttgctt ctcgccggct gcgtcacggc gcggctgcac agcgaggagg
     9541 agctggcgac cgtcgggcgc gattgcgggc tggcgctggg cgagttgttc caggacgaga
     9601 gcgagaaaaa gctggtgttc ctgttcaagc cgggcgcgtc gcgcgagcag cgcgcctgcg
     9661 tcgtccgctg ggcgcgcaag aaccacctga agacggtgat cgtcgaggcg atcaacttcc
     9721 ccgaagagag ctgagccgat gccccgccgc accgacattg cctcgatcct gatcatcggc
     9781 gcggggccga tcatcatcgg ccaggcggcc gagttcgatt attccggaag ccaggcgctc
     9841 aagacgctca aggccgaagg gtatcgggtg atcgtcgtca attccaaccc ggcgacgatc
     9901 atgaccgacc cagagctggc cgacgcgacc tacatcgagc cgatcacccc gggcatcgtc
     9961 gccaagatcc tcgagaagga gcggcccagg caccccggag gattcgccct gctgccgacc
    10021 atgggcgggc agaccgcgct caacaccgcc ctggcgctgg cgagggacgg cacgctcgag
    10081 cgattggggg tcgagctgat cggcgccaac gccgcggcga tcgagatggc cgaggaccgg
    10141 ctgaagttcc gcgaggcgat ggaccgcatc aggctcgaaa gcccgcgctc ggcgatcgcc
    10201 cacagcctcg acgacgcgct ggccgcgctc gacagcgtcg gcctgccggc gatcatccgc
    10261 ccgagcttca ccctcggcgg caccggcggc ggcatcgcct acaaccgcga ggagttcgag
    10321 gcgatcgtcc gcggcggcct cgaagcgagc ccgtcgagcg aggtgctgat cgaggaatcg
    10381 gtgctcggct ggaaggaata tgagatggag gtggtccgcg accgcgcgga caatgcgatc
    10441 atcatctgct cgatcgagaa cgtcgacccg atgggggtgc ataccggcga ttcgatcacc
    10501 gtcgcgccgg cgctcaccct gaccgacaag gaatatcagc gcatgcgctc ggcatcgatc
    10561 gcggtgcttc gggaaatcgg cgtcgagaca ggcggttcca acgtccagtt cgcaatcgac
    10621 cccaagaccg ggcggatggt ggtgatcgag atgaacccgc gcgtctcgcg atcgtccgcg
    10681 ctcgcgtcga aagccaccgg attcccgatc gccaaggtcg cggcgcgcct cgcggtcggc
    10741 tacacattgg acgagatcgc caacgacatc accggcggcg cgaccccggc gagcttcgag
    10801 ccgaccatcg attatgtggt gaccaaggtg ccgcggttcg cgttcgagaa gttccgcggc
    10861 gccgaggcga ccttgagcac gtcgatgaag tcggtcggcg aggtaatggc gatcggccgc
    10921 tgcttcgccg agagcctgca gaaggcgctg cgcgggctcg agaccgggct gaccgggctc
    10981 gacagggtgc gcgagctcga gcattccgac ccggcggcga tcgagagcgc gctggcgcgg
    11041 ccgacgcccg accggctgct ggtcgccgca gaggcgctcc gccagggctt cacggtcgag
    11101 cggatcaacg ccatcgccgg cttcgacccc tggttcctcg agcggttgct cgagatcgtc
    11161 gaggccgagg agcaggtgcg cagcgaaggg ctgccgcagg acgaggccgg gatgcgcgag
    11221 ctcaaggcga tgggcttttc cgacgcccgg ctcgccgagc tcgccttgcg ctccgccaag
    11281 gcgccgcgcg gcatgagcga gaagagcgcg cgcgggtcgg gcgtcgtcca cgatgcgctg
    11341 aaggcgctga ccggcggggt gaccgaggac gaggtgcggg cccaccgcct caagctcggg
    11401 gtgcggccgg tgttcaagcg catcgacagc tgcgccgccg agttcgacgc gaccacgccc
    11461 tacctctact cgacctacga ggcgccgctg ttcggcgagg ccgaggacga ggccgaggtt
    11521 tcggacaagc gcaaggtgat catcctcggc ggcgggccca accggatcgg gcaggggatc
    11581 gagttcgatt attgctgctg ccacgcggcg ttcgcgctcg gccgggaggg ggccgggctc
    11641 gagacgatca tggtcaattg caacccggag acggtctcga ccgaccccga cacctccgac
    11701 cgcctctatt tcgagccgct gaccgccgag gacgtgatcg agatcgtcga gcgcgagagc
    11761 gccaagggcg agctgctcgg ggtgatcgtc cagctgggcg gtcagactcc gctcaagctc
    11821 gccgccgagc tgcaggctgc gggcgtgacg atcctcggca ccagcccgga ttcgatcgac
    11881 ctcgccgagg accgcgagcg gttcgcccgg ctggtcgcgc gtctggggct gctgcagccg
    11941 cccaacggca tcgcccgaag ccgcgacgag gcgctgcagg tcgcccagcg gatcggctac
    12001 ccggtgcttc tgcgcccgtc ctacgtgctc ggcgggcgcg gcatggaggt ggtcgacggg
    12061 cccgcccagc tcgaccatta catcgccacc gcggtcgcgg tctcgggcag cagcccggtg
    12121 ctgatcgacc gctatctgcg cgacgcgatc gaggttgacg tcgacgcgat ctgcgacggc
    12181 aaggaggtgg cggtcgccgg agtgctccag cacatcgagg aggcgggggt ccattcgggc
    12241 gacagcgcct gcgcgatccc gccgcacggc ctgtcgagcg aagtggtcgc ggagatcgag
    12301 cggcagaccc gggcgctcgc gctggcgctc gacgtccggg gcctgatgaa cgtccagttc
    12361 gcggtcaagg aggagcaggt ctatctgatc gaggtcaacc cgcgcgccag ccggacggtg
    12421 ccgttcgtcg ccaaggcgat cgggcggccg atcgccaaga tcgcggcccg ggtgatggcc
    12481 ggcgagccgc tgtcggcgtt cgacccgatc gaccgcgaca tcggccatat cgcggtcaag
    12541 gaatccgtct tcccgttcgc gcgcttcccg gggaccgacc ccgtgctcgg gccggaaatg
    12601 aggagcaccg gcgaagtcat gggaatcgac cgggatttcg acatcgcctt cgccaaggcc
    12661 ttgctcgggg cggggatgga gctgcccgac ggcggaaccg ccttcgtgtc ggtcaaggac
    12721 gccgacaagg accatatcgt ccccgcggtc gagaaactgg tcgagctcgg cttcgcgatc
    12781 atcgccaccg gcggcaccgc cgggcacctc gccgagcgcg ggcttccggt caccccggtc
    12841 aacaaggtcg cccagggtcg gccgcacatc gtcgaccggc tgctcgacgg cgacgtcgac
    12901 ctggtgttca acaccaccga gggctggcag tcgctcaagg acagccattc gatccgggcg
    12961 acggcgctcg cccgcaaggt gccctatttc acgaccgcgg ccgcgagcct ggccgccgcc
    13021 cgctcgatcg aggcggtccg cgggcacgcg cttgaagttg cctcgcttca gtcctattat
    13081 tccgcttccg taaactgatc ttcagctgac aatccgctcc tcgatccgcg agccggcccg
    13141 gccgcgcagg agcgatggtt tggtaggaaa ggcatttttt caatggcgac tgccgacaag
    13201 gtgccgatgc tggccgaggg ccaccgcaag atcgacgagg aagtccggca tttgaagacc
    13261 gtcgagcgcc ccgccgcgat cgaggcgatc gagatggcgc gcgcccatgg cgacctcgcc
    13321 gagaacgccg aatatcatgc cgccaaggag cgccagggcc agatcgaggc gaccatcgcc
    13381 gacctcgagg accggctcag ccgggcccag gtgatcgacc cgacgaccct gtcgggcgac
    13441 aaggtggtgt tcggcgcgac cgtcaccctg atcgacgagg acaagaagaa gatccgctac
    13501 cagctcgtcg gccagaccga ggccgacgcc aaggtcgggc ggatcagcta caattcgccg
    13561 ctcgggcggg cgctgatcgg ccgccatgcc ggcgaggagg tcgaggtctc gaccccgtcc
    13621 ggcgaccgct actacaagat caaagccatc gaattcgtct gagcggggca cgaagcgcgt
    13681 gcggatcccg cggacggcca ccatcgcact cgcgggcgtc accgcgctcg cctggctggt
    13741 ggtcgcggcg atcggcgccg atgaccacgc ggcggcgctg tggggcttca tcccggcgcg
    13801 gttgagcggg ctgctcgacc tgccggcggc ggtgcccgcg gcgctgaccc cgctgagctc
    13861 gaccctggtc cacggcgggc tgctccacgt cgcgttcaac ctgctgatgc tgctgtggtg
    13921 cgggaccgcg gtcgagcggg tgctcgggcg cggcgccctg ctgctgctgt acgtcgtcgg
    13981 cgcttatgcc gcggcggtgg cgcaatggct cgccgacccg gcctcgatcg tgccggtgat
    14041 cggcgccagc ggagcgatca gcgcggtgat cggggccttc gcgctgagct tcggcgaggc
    14101 ccgaaggatc acccgctcgc cgcgcctcaa ccggctgctc aacgccgcct ggctgctcgc
    14161 cgcctgggtg gtgatgcaga tgatgatcgg ctggctggcc ggcgggcgcg ggctactgct
    14221 cgccacgccc gcccatgtcg gcggcttcct cgccgggctg ctcctgcagc ggccgctgct
    14281 tctgtggcgc taccgctccg cctaggcgct ttctgcggcc ggcgccgctg aagccgattc
    14341 ggcgtccggc tccaggagcc ggtgcaagtg gacgacgaca tatttcatct cggcatcgtc
    14401 gaccgtccgc tgggccttgc cgcgccaggc atcctcggcc tcggcgtagc tcgaatagag
    14461 accgaccagg tcgatgctgt cgaggtcgac gaactcgagg ccgcgcgggt ccttgacccg
    14521 cccgccgaac accaggtgga gcttgctcat ccgcgggtcc gggccgcgtc cagcgcggcc
    14581 tgcgccgagg tcttcgcggc gtcgctcgcg ccctcgaaga tcgaacgcag cgtctcggcg
    14641 cccttttcac gggtcaggcc cagctcggcg aggcgcgagg tgcccgcttc ccgggccgct
    14701 tcggccgcgg ccctggcggt gccggtgagc ttgtccgcgg tcggccgcag cagctgcgtc
    14761 tcgctttgcg tgcggggcag gagggccgcg atcagcgccc cggccgcaag cccgccggca
    14821 agcgcgagca gcggcgcttc gtcgatcgcg tcggtcgcct tgcggcccgc cccggcgact
    14881 cggccgcggg cgccgtcata agcctcgatc gcgcgccgtc ggaccccgct ctcgcggtcc
    14941 tggcgttcct tgtcctggtc agtcattcga tcgtctccgt ctttttcgct ttgctcttgg
    15001 tcttgcgcgg ggccgccttg cccttgcggg ccttgcgctt ggcggtggct ccgccggcga
    15061 gcttgccggc catgtcgatc agcggctcgc gggcgaggaa cagggccagc gccgcgacga
    15121 ctccgccggc gatcgcgggc cgccgccgaa ccgcgtcgac cgcttcctcg gccaggtcgg
    15181 cgcccttcga cttggcgccc gcccatgcgt tctgcgccag ggttccgggg ctcaggcgct
    15241 cctggagctc gcgcgcggtc tccatcagcc gcgcccgcgc ccgctcggcc tcgatccgcg
    15301 ccgcggcgac attgggagca tcgccgctca taggtcgcgc ttcgcctttt cgattccgaa
    15361 ccaggcgagg gccgcggcgc cgccggcgaa gaccaggaac gcgagcaagc ccgcgaggat
    15421 cgggcccatc agcgcgagca ggccgaggaa caccgcgacc gcgagcaccg tgaccgccgc
    15481 ctgggcgaac aacagcgccg cgccgagcag gatcgccggg accctgagac gatcggcctt
    15541 cgccgacgcg atcgccctgg cgaccccgag ctccgcctgc gcataggcct tgccctcctc
    15601 gacgagctgg tggacgagct cgccgatcgg gcgctcgtca ccgggctccg gcggctcggg
    15661 cggctgcctg gccgggtcgt tcggcttcag catcagctct cccgcttgcc ccgcgaacgg
    15721 ctgacgtcct cgtcctcgtc gcgcgcgagt cccgacttga tcaggcgggc gagcgcgaag
    15781 ccgacgatcg ccgctccggc gagcgcgact cccgggctct tgcggacgaa ctcgcgggtg
    15841 tcgtcgatca gctcgtcggg atccttgccg gcaaggttgt tggcggcgtc ctcgatggcg
    15901 gtcgccgcgc ggcgcgcata atcgccatat tcgggcccga gccgctcatc gaggccgtct
    15961 gcggtgtcgc cgaccagctt gccgacgttg gccagcgctt ccgcgccgcg ctcgagcccc
    16021 tggccgacca gcccgcgggt cttgtcggcc gcctggcccg ccagcttctc gcggccggtg
    16081 cggatcttgt cgacgatcac gtcgcgggtc gcggcccggc cggtgcgctc accggcgggc
    16141 tgggcggtcg gctggccggt gtcggcgacc agcagcacgt cgccgtcctc gccctcggcg
    16201 gacgtggggg tcgaagcgcc ggagacgacc ttgtcggtgc cctcggggag ctcgcgttca
    16261 ttgtcctgca tctcatgtcc tcctttagag gcctcaaccc tgagccggga cgcttcgttc
    16321 cggcacgtgg gagcgaatgt tgcacgcctc gacccgggtc atataagtgc gcttcggccc
    16381 ggtttcatta cgtccccgca agaaagattt tccatgcccg ccatcgtcga cgtccacggc
    16441 cgccagatcc tcgacagccg cggcaacccg acggtcgagg tcgacgtcac gctcgaggac
    16501 ggcagcatgg gccgcgccgc ggtgccgtcg ggggcctcga ccggcgccca tgaggcggtc
    16561 gagctgcgcg accgcgacga cgcccgctgg tgcggcaagg gggtcgaagg ggcggtgcgc
    16621 tcggtcaacg gcgagatcgc ggaagccatc gtcggccgcg aggccgggga ccaggccgac
    16681 atcgacgcgg cgctgatcgc gctcgacggc accgccaaca agagccggct gggggccaac
    16741 gcgatcctcg gggtcagcct ggccagcgcc aaggcggccg cggaatcgct cgggctgccg
    16801 ctctaccgct acgtcggcgg ggtcggcgcc gagctgctgc cggtgccgat gatgaacatc
    16861 ctcaatggcg gcgcccacgc cgacaatgcg atcgacttcc aggaattcat ggtcatgccg
    16921 gtcggcgctc cgagcttttc cgaagctttg cgctgcggcg ccgagatctt ccacgcgctc
    16981 aagagcgcgc tcgccgcggc cggcctgtcg accgcggtcg gcgacgaggg cggcttcgcg
    17041 ccggccattt cctcgtcccg cgaggcgctc gacctgatca tgcgggcggt ggccagcgcc
    17101 gggtacgcgc ccggcgacca tgtgctgatc gcgctcgact gcgcggcgac cgaatatttt
    17161 cacgacggcg gctaccggct ggaaggcgag ggcaggagct tcacgcccga ggcgatgacc
    17221 gactatctcg ccgagcttgc cgacgcttac ccgatcgcgt cgatcgagga cggcatggcc
    17281 gaggacgaca tggccggctg gaagcaactg accgagcggc tcggcggccg cgtgcagctg
    17341 gtcggcgacg atctgttcgt gaccaacgag cagcgcctcg ccagcggcat cgccggcgga
    17401 atcgccaact cgatcttggt caaggtcaac cagatcggca cgttgaccga gaccatcgcc
    17461 gcggtccggc tggcgcaggg ctcgggctac accgcggtga tgtcgcaccg ctcgggcgag
    17521 accgaggaca cgaccatcgc cgacctcgcg gtggcgctcg gctgcgggca gatcaagacc
    17581 ggcagccttg cgcgctccga ccgcaccgcc aagtacaacc agctgctgcg catcgaggag
    17641 gagctcggcg agagcgcgcg ctacgccggc cgcgccgcgc tcaaggcctt tgtcacccgc
    17701 tgatccacag ctttatccac caaagcattc ttttcgcttg ccttcatgac gtcatcgtga
    17761 ttccttgctg cgcatggggg cagctcagca agccagggga ttgatccgcc gagcggcgat
    17821 gccggcgctg gcgctgatcg tggtcggcaa tttcgccggc tatgcgatcg ccggccccaa
    17881 cgggctgctc gcctggggcg gctatcaccg cgacctcaac gagcgaaagg ccgagctcgc
    17941 ccgactcgag gccgagcgcg cccagcttcg ccaccgctcg gcgctgctcg acccgaagaa
    18001 ggccgacccc gacatcgccg acgagctggt tcgccgcgac ctcggcctgg tccgccccga
    18061 cgaagtcatc atcccgctcg aataaagcgc ccggcggttt ccagcagggc cgtggttgcc
    18121 agttgggccg cggctttcct atagagcctg ccgcctccta gccccacgaa aggccacgcc
    18181 ttggcgcgcg cagcaaagcc gaagtcgaaa cccgccgcag cgccgccgcc ggccaaccgg
    18241 gccaaggcgg cgtccccgcc gccgcgcaag gcacccaacc gggaacgccc gcccgagccc
    18301 aagcgccaca aggcggagcg cgacgagctg ctcggcctct acaagcagat gcttctgatc
    18361 cgccgcttcg aggagcgggc ggggcagctg tacggcctcg ggctgatcgg cggcttctgc
    18421 cacctctaca tcggccagga ggcggtcgcg gtcggcctcc agtcggcgat gacggtcggc
    18481 aaggacagcg tgatcaccgg ctaccgcgac cacggccaca tgctcgccta cgggatcgac
    18541 cccaagctga tcatggccga gctgaccggg cgcgccgccg gcatctcgaa gggcaagggc
    18601 ggctcgatgc acatgttctc ggtcgagcac ggcttctacg gcggccacgg catcgtcggg
    18661 gcgcaggtca gcctcggcac cgggctcggc ttcaagcacc gctacgcaga ggacggcggg
    18721 gtgtgcctga cctatttcgg cgacggcgcc gccaaccagg ggcaggtcta tgaatgcttc
    18781 aacatggcgc agctgtggaa gctgccggtg atctacgcga tcgagaacaa ccaatatgcg
    18841 atgggcacca gcgtcgagcg gtcgtcgtcc gagcccgatt tctacaagcg cggggaaagc
    18901 ttccggatcc ccgggctgcc ggtcgacggg atggacgtgc tcgccgttcg cggcgcggcc
    18961 gagacggcgc tcgaatggac tcggagcggc aagggcccga tcatcctcga gctgaagacg
    19021 taccgctacc gcggccattc gatgtccgac ccggccaagt accggacccg cgaggaggtg
    19081 caggaattcc gcgagcacaa cgacccgatc ggcaacgcag cccgggacct cgccaagctc
    19141 ggggtcggcg aggacgaact gaaggcaatc gacaagcaga tcaaggatat cgtcgtcgaa
    19201 gctgctaagt ttgctgaaga cgctccgctg cccgacgggt ccgagctcta caccgacgtc
    19261 ctggtcggga gctactgatg gcgaccgagc tcaccatgcc cgcgctctcg cccaccatgg
    19321 aggaggggac gctcgccaaa tggctggtca aggaaggcga cgaagtgaag tccggcgaca
    19381 tcctcgccga gatcgagacc gacaaggcga cgatggaatt cgaggcggtc gacgagggca
    19441 cgatcgcgaa aatcctggtt ccggagggca gcgacggggt gaaggtcggc accccgatcg
    19501 cggtgctcac cgcggacggc gaggatgcga gcgccctgcc ggctgccccg aagcatgacg
    19561 aaactccgca gccgacgccg tccgaccaga agaccgaggc tgctcccatc gagaccgagc
    19621 agccgcaaaa ggccgagacc ggaaccgccc agctggccgc ggcccccgcg gccccggccg
    19681 gccccgaagt gcccgagggc accgcgctcg ccccgctcag cgtccgcgag gcgctgcgcg
    19741 acgcgatggc cgaggagatg cgccgcgacg agcgcgtgtt cgtgctcggc gaggaggtcg
    19801 ccgagtacca gggcgcctac aaggtcaccc agggcctgct cgaggagttc gggcccaggc
    19861 gggtcgtcga cacgccgatc accgaatacg gctttgccgg cctcggcacc ggcgccgcga
    19921 tgggtggctt gcgcccgatc gtcgagttca tgaccttcaa cttcgcgatg caggccatcg
    19981 accacatcgt caattcggcg gccaagacca attacatgtc gggcggccag atgcgctgcc
    20041 caatcgtgtt ccgtgggccc aacggcgccg cggcccgcgt ggccgcgcag cacagccaga
    20101 attacggcgc ctggtatgcg agcgtgccgg ggctggtggt gatcgcgccc tacagcgccg
    20161 cggacgcgaa ggggctgctc aaggccgcga tccggacgga cgacccggtc gtcttcctcg
    20221 agaacgagct gctctacggc cagcatttcg acgttccgca gatggaagac tatgtcctgc
    20281 cgatcggcaa ggcgcggatc gtgc
//
LOCUS       DASTZO010000122         3573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_532502,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000122 DASTZO010000000
VERSION     DASTZO010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532502"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VFU20_03470"
     CDS             <1..768
                     /locus_tag="VFU20_03470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285557.1"
                     /translation="CGSQKTYDLIKRELFRRAAQVRGSDQAAFDRLATYSVVRMDAPM
                     LRSHDEELGTVSCAGSLALDLPPGVAVVGGRRTLNANIGYAIQPAADGSGDVVTLSNA
                     DAIITPLATLARVAVPAEDPLAPAPAGSAPVVIPPLTGEPAPMPAPVAPPSDTGPPPP
                     QPTASPSFNCRTARSRGEIAVCNDPGLAALDRQMAAQFSTALAQADSGQRALLQRTRS
                     DFLRYRDNCPSNACIAETYRGRMREIRDIMAGRWQSR"
     gene            841..>3573
                     /gene="dnaE"
                     /locus_tag="VFU20_03475"
     CDS             841..>3573
                     /gene="dnaE"
                     /locus_tag="VFU20_03475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5285558.1"
                     /translation="MTAPYVPLRNLSCFTMLEGAMEPKVIAERAAALGFPAIALTDRN
                     GLYAAMPFSDSCAAKGVQPIVGAMLAVAPPAEIGSGDRIDWLVLLAKDEAGYSNLCRL
                     VSSAHLDRPLSEEPHVALSALDGASDGLIALTAGSEGALARLIADGQSAKARDYLGRL
                     EALFPGRLYVELSRRGDPVEDAAETALVELAYERDLPLVATNPAAYTDPSFHAAHDAL
                     LCIAHSAYLESAERVTSSSEAWLKDGAAMAELFADLPEAIANTSVIAQRCAIAAPKRQ
                     PILPRLSEDEDETLRRDARAGLAERLQGRDPDEQEAYRKRLEFELDVITGMGFAGYFL
                     IVADFIKWAKGEGIPVGPGRGSGAGSLVAWALTITDLDPIALNLLFERFLNPERVSMP
                     DFDIDFCETHRDAVIAYVQRKYGRDRVAQIITFGRLKARAVLKDTGRVLQMSYGQVDR
                     LAKLIPNHPTDPWTLERTLGKARDKDGNLLAGVPELIAEYRDDSDVRRLFDLAMKLEG
                     LPRHASTHAAGVVIGDRRLDELVPLYRDPRSEMPVTQFDMKYVEAAGLVKFDFLGLKT
                     LSVLAEGRRMLAGQGVHVDLNALSWDDPEVFELLQRGDTVGVFQLESEGMRRTLAAVR
                     PTSFGDIVALVSLYRPGPMDNIGMFGDRKNGRVPIAYPHPMLEEVLEETYGIFVYQEQ
                     VMQAAQVLAGYSLGEADLLRRAMGKKIQSEMDAQRSRFVEGAAVHGISAAKASELFDL
                     IDKFAGYGFNKSHAAAYAVVAYQTAWLKAHHRPEFYAASMSFDMAQTDKLALFVEDMR
                     RAGVDCLPPDINASRADFAVEGEAVRYALGALKGVGEKAMEALVAEREAHGPFASLDD
                     FAARIDPRLLNRRQIESLAGAGAFDSLDPDRAALFAGAETILAHAASEHDQRTSG"
BASE COUNT          551 a         1319 c         1187 g          516 t
ORIGIN      
        1 tgcggctcgc agaagaccta cgacctcatc aagcgcgagc tgttccgccg cgccgcccag
       61 gttcgcggca gcgaccaggc ggcgttcgac cggctcgcca cctattcggt ggtgcggatg
      121 gacgcgccga tgctccgcag ccacgacgag gagctcggaa cggtcagctg cgccggctcg
      181 ctggcgctcg acctgccgcc cggggtcgcg gtggtcggcg gacgccgcac cctcaatgcc
      241 aacatcggct atgcgatcca gcccgccgcc gacggcagcg gcgacgtcgt caccctgagc
      301 aacgccgatg cgatcatcac cccgctcgcg accctggccc gggtcgccgt cccggccgag
      361 gacccgctcg ctccggcccc ggcgggttcg gccccagtgg tcatcccgcc gctgaccggc
      421 gagcccgcgc cgatgccggc cccggtggct ccgccgtcag acaccggccc gccgccgccg
      481 cagccgaccg ccagcccgag cttcaactgc cgcaccgccc gcagccgcgg cgagatcgcg
      541 gtgtgcaatg acccgggcct cgccgcgctc gaccggcaga tggccgccca gttcagcacc
      601 gctctcgccc aggccgattc gggccagcgg gcgctgctcc agcgcacccg ctccgatttc
      661 ctgcgctacc gcgacaattg cccgtccaac gcctgcatcg ccgagaccta ccgcggccgg
      721 atgcgcgaga tccgcgacat catggccggg aggtggcaat cgcgctagcg gcgcccaatc
      781 agttgtggat aagcctgccg agcggttttc ctcgtccgcc ggacgcgcca tagtcgcggc
      841 gtgaccgcgc cctacgtccc gctccgcaac ctgtcctgct tcaccatgct cgaaggggcg
      901 atggagccca aggtcatcgc tgagagagcc gcggcgctcg gtttcccggc gatcgccttg
      961 accgaccgca acgggctcta cgcggcgatg ccgttcagcg attcctgcgc ggccaagggc
     1021 gtccagccga tcgtcggagc gatgctggcg gttgctccgc cagccgagat cggcagcggc
     1081 gaccggatcg actggctggt gcttctggcc aaggacgagg cgggctattc gaacctgtgc
     1141 cggctggtct cctcggccca cctcgaccgg ccgctgtccg aggagccgca cgtcgctttg
     1201 tcggcgctcg acggcgccag cgacgggctg atcgcgctca ccgccggctc ggaaggcgcg
     1261 ctcgcccggc tgatcgccga cggccagtcg gccaaggcgc gcgattatct cggccggctc
     1321 gaggcgctgt tccccggccg gctgtacgtc gagctgagcc gccgcggcga tcccgtcgag
     1381 gacgccgccg aaaccgcgct ggtcgagctc gcctacgagc gcgacctgcc gctggttgcg
     1441 accaaccccg ccgcctacac cgacccgagc ttccacgccg cgcacgatgc tttgctgtgc
     1501 atcgcgcatt cggcctatct cgaaagcgcc gagagggtca cctcgtcgtc cgaggcctgg
     1561 ctcaaggacg gagcggcgat ggccgagctg ttcgccgacc tgcccgaggc gatcgccaac
     1621 acgtccgtga tcgcccagcg ctgcgcgatc gccgcgccga agcgccagcc gatcctgccg
     1681 cgcctgtccg aggacgagga cgagaccctg cgccgcgacg caagggccgg gctcgccgag
     1741 cgcctgcagg gccgcgaccc cgacgaacag gaggcctacc gcaagcgcct cgagttcgag
     1801 ctcgacgtga tcaccggaat gggctttgcc ggctacttcc tgatcgtcgc cgacttcatc
     1861 aaatgggcga agggcgaggg catcccggtc ggccccggcc gcggctcggg cgccggctcg
     1921 ctggtcgcct gggcgctgac catcaccgac ctcgacccga tcgcgctgaa cctgctgttc
     1981 gagcgcttcc tgaaccccga gcgcgtgtcg atgccggact tcgacatcga cttttgcgaa
     2041 acccaccgcg acgcggtgat cgcctacgtc cagcgcaagt acggccgcga ccgggtcgcc
     2101 cagatcatca ccttcgggcg cctgaaggcg agggcggtgc tcaaagacac cgggcgcgtc
     2161 ctgcagatga gctacggcca ggtcgaccgc ctggccaagc tgatccccaa ccatccgacc
     2221 gacccgtgga cgctcgagcg cacgctcggg aaagcccgcg acaaggacgg caacctgctc
     2281 gcgggagtgc ccgagctgat cgccgagtat cgcgacgatt ccgacgtccg gcggctgttc
     2341 gacctggcga tgaagctgga ggggctgccg cgccatgcct cgacccacgc ggcgggcgtg
     2401 gtgatcggcg accggcggct cgacgagctg gtgccgctgt accgcgaccc gcgctcggaa
     2461 atgccggtca cccagttcga catgaaatat gtcgaggcgg cggggctggt gaagttcgac
     2521 ttcctcggcc tgaaaacgct gtcggtgctc gccgagggcc ggcggatgct cgccggccag
     2581 ggcgtccacg tcgatttgaa tgccctgagc tgggacgacc ccgaggtgtt cgagctgctc
     2641 cagcgcggcg acacggtcgg cgttttccag ctcgaatccg aaggcatgcg ccgaaccctc
     2701 gccgcggtcc gcccgaccag tttcggcgac atcgtcgcgc tggtttcgct ctaccgcccg
     2761 ggcccgatgg acaacatcgg catgttcggc gaccgcaaga acggccgggt gccgatcgcc
     2821 tacccgcacc cgatgctcga ggaggtgctc gaggaaacct acggcatctt cgtctaccag
     2881 gagcaggtga tgcaggccgc gcaggtgctc gccggctaca gcctcggcga ggccgacctg
     2941 ctgcgccgag cgatgggcaa gaagatccag agcgagatgg acgcccagcg cagccgcttc
     3001 gtcgaaggcg ccgcggtcca cggcatttcc gccgccaaag cgtccgagct gttcgacttg
     3061 atcgacaagt tcgccggcta cggcttcaac aagagccacg cggccgccta tgcggtggtc
     3121 gcctaccaga ccgcctggct caaggcgcac caccggcccg aattctacgc ggcgtcgatg
     3181 agcttcgaca tggcccagac cgacaagctc gctctgttcg tcgaggacat gcgccgcgcc
     3241 ggggtcgact gcctgccgcc cgacatcaac gccagccgcg ccgattttgc ggtggaaggc
     3301 gaggcggtcc gttacgcgct gggcgcgctc aagggtgtcg gcgagaaagc gatggaggcc
     3361 ctggtcgccg agcgcgaagc ccacggaccg ttcgcaagcc tcgacgattt cgccgcgcgg
     3421 atcgacccgc ggctgctcaa ccgccgccag atcgagagcc tcgccggtgc aggtgccttc
     3481 gactcgcttg accccgaccg cgcggcattg ttcgcgggcg ccgagaccat cctagcccat
     3541 gcggcgagcg agcacgacca gcgcaccagc ggg
//
LOCUS       DASTZO010000123         4916 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_480489,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000123 DASTZO010000000
VERSION     DASTZO010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_480489"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="VFU20_03480"
     CDS             <1..243
                     /locus_tag="VFU20_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285559.1"
                     /translation="ASPPLATRITPSWMTFPIGGDFSTRKRVRVGSVIARGGAPGVVE
                     TDHPAAVRLAEIGGRGQADSGGNQSERPKGPGHASG"
     gene            435..896
                     /locus_tag="VFU20_03485"
     CDS             435..896
                     /locus_tag="VFU20_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA2169 family four-helix-bundle protein"
                     /protein_id="HEU5285560.1"
                     /translation="MTEFTRQDEITTLNTLIATLIDSVTGFEDAASNIEGTSRFQELF
                     RERASERRRVVEDLRAEVRRLGGNPEDDGSFLGKAHQRFLDLKSVITGRDEQAIINEV
                     ERGEDYLKEKFETALSSGSLSGESRAVVERAYQSVRSGHDQVSALKHGLEA"
     gene            1042..1407
                     /locus_tag="VFU20_03490"
     CDS             1042..1407
                     /locus_tag="VFU20_03490"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5285561.1"
                     /translation="MRPTLLLAAAALLAAGPAAAQPVQTVTLYSHGYAPTVLRLAAGQ
                     PVTLVFVNQAGKSHDFTARRFFHSAQILSGRVYGGEVNLRGGQSARVTLIPRAGRYPV
                     HCGYPFHKMLGMRGTIIVQ"
     gene            1980..4328
                     /locus_tag="VFU20_03495"
     CDS             1980..4328
                     /locus_tag="VFU20_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU5285562.1"
                     /translation="MAESSPAIPDDVGSPAYLQGLNPPQRQAVLTTEGPVLVLAGAGT
                     GKTAALTARLAHIIATRRAWPSQILAVTFTNKAAREMRERVSAISGGAIEGMPWLGTF
                     HSVAARMLRSHAELVGLKSNFTILDTDDQLRVLKQLIQAANLDEKRWPARQLAGRVDR
                     WKNRGWTPSQVEPGEAEIFANGRGAELYAAYQDRLRTLNACDFGDLLLHMLVIFRRHE
                     DVLAAYRERFKYILVDEYQDTNQAQYEWLKLLAEPRRNLCCVGDDDQSIYSWRGAEVA
                     NILRFDRDFPGAAIIRLEQNYRSTTHILAAASGLIAHNSGRLGKTLWTDVGDGEPVKV
                     IGVWDGPEEARRVGEEIEGYQRAGGSLDDAAILVRAQFQTREFEERFIAIGLPYQIIG
                     GFRFYERAEIRDALAYLRLIAQPADDLAFERIVNQPKRGLGDIAVARIHAFARATQQP
                     LLLAAAQMLDSDELTPQARRSLGNFVADIARWRGMISAPAFAGEGDHAKHGGGVPHPE
                     LARILLDESGYTAMLQADRSAESAGRLENLAELARAMEEYESLGAFLEHVSLVMDNDE
                     ARAGEKVTIMTIHAAKGLEFDRLFLPGWEEGLFPSQRAMDEGGLASLEEERRLAYVAI
                     TRARRAATILHAANRRIYGQWTSSLPSRFLSELPSEHLEEETTMTGGESLWRAQWSER
                     SDPFAHVAALRPSRAQTRGPGWQRAAGGGAFSTQPERIIEARASAVSLGNKGRDDLAL
                     GQRVFHGKFGYGTIAAIEGNKLEIDFEHSGRKKVLDSFVSVE"
     gene            4325..>4916
                     /locus_tag="VFU20_03500"
     CDS             4325..>4916
                     /locus_tag="VFU20_03500"
                     /inference="COORDINATES: protein motif:HMM:NF024859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5285563.1"
                     /translation="MSHFRAALLTLVLIGCTSREPGAPARIDHVIVGVSDLERGVREI
                     ERLTGVRAVTGGAHPGQGTRNALLSLGEDSYLEIYAPNPVEPVSSPAVEELRALDRPT
                     PIGWAASTSDSRSLRSTLESKGFPLSQPERGSRRRPDGSSLSWVTYGFAKLDHPLAPF
                     FIEWGDARLHPSRTSPEGCSLRSLRIEDPAADRLRRA"
BASE COUNT          823 a         1771 c         1581 g          741 t
ORIGIN      
        1 gcctcgcctc ccttggcgac ccgtatcacc ccgagctgga tgacgtttcc gatcggcggc
       61 gacttcagca ccaggaagcg ggtccgagtc ggttccgtaa ttgcgcgagg cggtgcaccg
      121 ggcgtcgtcg aaactgacca cccagcggcc gttcggctgg cggagatcgg cggccgcggc
      181 caggccgatt ccggcggcaa tcagagcgaa cgcccgaaag gtcctggtca tgcttcgggc
      241 tagcaaggtt cgcccgccgc aacaatgagt tgggcgcgag ttggccgaga caacccctgt
      301 cctcggcggc gtcgggttcc gctcggctgc ccacaggcgc cgctcggctc aaaaaagtac
      361 cgcggcgcgc atcgcctccg cggctcgagc gttccttgag cattccaaca atcttgaacg
      421 cgggagcttc ttccatgacc gaattcacac gccaggacga aatcaccacc ctcaacaccc
      481 tgatcgcgac cctgatcgac agcgtcaccg gcttcgagga cgccgcgtcc aacatcgagg
      541 gcaccagccg cttccaggag ctgttccgcg agcgcgccag cgaacgccgc cgggtcgtcg
      601 aggacctgcg cgccgaagtc cgtcggctgg gcggcaatcc cgaggacgac ggctcgttcc
      661 tcggcaaggc gcaccagcgc ttccttgacc tcaagtcggt gatcaccggc cgcgacgagc
      721 aggcgatcat caacgaggtc gagcgcggcg aggactatct caaggagaag ttcgagaccg
      781 ccctcagctc cggctcgctg tcgggcgaaa gccgggcggt ggtcgagcgc gcctatcagt
      841 cggtccgctc gggccacgac caggtcagcg ccctgaagca cgggctcgag gcctaggccc
      901 agggaaacca atcccgcccg aaccagtggg cgggggcacg gggcggcggc acgcgaacgc
      961 accgccgccc cggttgtgtg ggccgatagc aagcgttcag cttggcgtgg tagaatgggg
     1021 cttcaaacca ggaggacacc catgcgtccg acgcttttgc tggccgccgc cgcgctgctc
     1081 gccgccggcc cggcagccgc ccagcctgtc cagacggtca cgctctacag ccacggctac
     1141 gcgccgacgg ttctgcgcct cgccgcgggg cagcccgtga ccctcgtctt cgtcaaccag
     1201 gcgggcaaga gccatgattt cacggcccgg cgattctttc actccgccca aattctctcg
     1261 ggccgagtct atggcggcga ggtcaacctc cgcggcgggc agagcgcaag ggtcaccttg
     1321 atcccgaggg ccggccgcta cccggtccat tgcggctatc cgttccacaa gatgctcgga
     1381 atgcgcggca cgatcatcgt gcaatgagcc cgaagctcaa gcggtaagga atgccgccgc
     1441 ggatagcgca catggaaatg ggggccggca ttgctgccag cccccactgc gccgagcccg
     1501 gatatgccgg tgttctcgtt ccggatcggc cgaagcctca ccgtctctcg atcctggctc
     1561 accagctcag gcgtcgcttc ccatcgtctt tggtccgaag acctccggca atgttccgcg
     1621 gcccttcttg ggatgaccgc ttctgcgtcc cgctttgccc ccgctgtccc gaaggccagc
     1681 tgaagccgcg tcgcgcaaaa ggaagatcat ctcttccggc gcctcttccc ggctggcccc
     1741 ggttcaggtc cgaagaccct ccccactgcc tgccggcaga gatcggaacc ttggtcacct
     1801 gccgcccgaa ggtggcctgc gaccgccgtc ccgatcaccc gatgatcaat gcactgcggc
     1861 ccgagtcgcg caaagcgaaa aatggatgcg cgagcctgtg gataacgggg atatcgggaa
     1921 caacagccgg aactttcccg ccattgcccg aatcggcccc ggcccctacc tcctcgcaag
     1981 tggctgaatc ttcccccgcg atcccagacg acgtcggctc gcccgcctat ctgcaaggcc
     2041 tgaacccgcc gcagcgccaa gcggtgctga ccaccgaggg cccggtgctg gtgctggccg
     2101 gggccggcac cggcaagacc gccgctctca ccgcgcgcct cgcccacatc atcgcaaccc
     2161 gccgcgcctg gccgagccag attctcgccg tcaccttcac caacaaggcg gcgcgcgaga
     2221 tgcgcgagcg cgtgtcggcg atcagcggcg gggcgatcga gggcatgccg tggctcggca
     2281 ccttccactc ggtcgccgcg cggatgctgc gcagccacgc cgagctggtc ggcctgaagt
     2341 ccaacttcac gatcctcgac accgacgatc agctgcgcgt gctcaagcag ctgatccagg
     2401 cggccaacct cgacgagaag cgctggccgg cgcgccagct cgccgggcgg gtcgaccgct
     2461 ggaagaaccg cggctggaca ccttcgcagg tggagcccgg cgaagcggaa atcttcgcca
     2521 acggccgcgg cgccgagctt tacgccgcct atcaggaccg gctgaggacc ctcaacgcct
     2581 gcgacttcgg cgacttgctg ctccacatgc tggtcatctt ccgccgccac gaggacgtcc
     2641 tcgccgccta tcgcgagcgg ttcaaataca tattggtcga cgaatatcag gacaccaacc
     2701 aggcccagta cgaatggctc aagctgctcg ccgagccgag gcgcaacctg tgctgcgtcg
     2761 gcgacgacga ccagtcgatc tattcgtggc gcggcgccga ggtcgccaac atcctgcgct
     2821 tcgaccgcga ctttcccggc gcggcgatca tccggctcga gcagaactat cgctcgacca
     2881 cgcacatcct cgccgccgcc tccggcctga tcgcgcataa ttcgggacgg ctcggaaaga
     2941 cgctgtggac cgatgtcggc gacggcgagc cggtcaaggt gatcggagtc tgggacggcc
     3001 cggaggaagc gcgccgggtc ggcgaggaga tcgagggcta ccagcgcgcc gggggctcgc
     3061 tcgacgatgc cgcgatcctg gtccgcgccc agttccagac ccgcgagttc gaggagcggt
     3121 tcatcgccat cggcctgccc tatcagatca tcggcggctt tcgtttctac gagcgggccg
     3181 agatccgcga cgcgctggct tacctgcgcc tgatcgccca gccggccgac gacctcgcct
     3241 tcgagcgcat cgtcaaccag cccaagcgcg ggctcggcga catagcggtc gcccggatcc
     3301 atgccttcgc ccgcgccacg cagcagccgc tgctgctcgc ggccgcgcaa atgctcgata
     3361 gcgacgaatt gaccccgcag gcgcggcgca gcctcggcaa tttcgtcgcc gacatcgccc
     3421 gctggcgcgg aatgatttcc gccccggcat ttgccgggga gggggaccat gcgaagcatg
     3481 gtggaggagt cccccacccc gagctcgccc gaatcctgct cgacgaaagc ggctacacgg
     3541 cgatgctcca ggccgaccgc agcgcggaat cggcggggcg gctggaaaac ctcgccgagc
     3601 tcgcccgcgc gatggaggaa tatgaaagcc tcggcgcgtt cctcgagcat gtcagcctgg
     3661 tgatggacaa tgacgaggcc cgggccggcg agaaggtcac gatcatgacc atccacgccg
     3721 ccaaggggct cgagttcgac cggctgttcc tgcccggctg ggaggagggc ctgttcccct
     3781 cgcagcgggc gatggacgaa ggcggcctcg cgtccctcga ggaggagcgc cgactggcct
     3841 acgtcgcgat cacccgcgcg cggcgtgccg cgaccatcct ccacgccgcc aaccgccgca
     3901 tttacggcca gtggacgagc tcgctgccga gccggttcct gtccgagctg cccagcgagc
     3961 atctcgagga agagacgacg atgaccggcg gcgagagcct gtggcgcgcg caatggagcg
     4021 agcgctccga cccgttcgcg cacgtcgccg cgctgcgccc gtcgcgggcc cagacccgcg
     4081 gacccggatg gcagcgcgcg gccggcggcg gcgcgttcag cacccagccc gagcgcatca
     4141 tcgaagcccg cgcgtcggcc gtgtcgctcg gcaacaaggg ccgcgacgac ctcgcgctcg
     4201 gccagcgcgt attccacggc aagttcggct acggcaccat cgccgccatc gaaggcaaca
     4261 agctcgaaat cgacttcgag cactccggcc gcaagaaggt gctcgacagc ttcgtgagcg
     4321 tggaatgagc cattttcgtg cggcgctgct gacactcgtg ctgattggct gcaccagccg
     4381 cgaaccaggc gcgcccgccc ggatcgacca tgtcatcgtc ggagtcagcg acctggagcg
     4441 cggcgtccgc gaaatcgagc ggctgacggg cgttcgagcc gtgaccggcg gcgcgcaccc
     4501 cggacaaggg acgcgcaacg cgctgctttc gctcggcgag gacagctatc tggaaatcta
     4561 cgcgcccaat cccgtcgagc ccgtctcgtc acccgcggtc gaggagcttc gcgccctcga
     4621 tcgtccgaca ccgatcggct gggccgcctc gacgagcgat tcccggtcgc ttcgctcgac
     4681 gctcgaatcg aagggcttcc cgctgtcgca acccgagcgc ggttcgcgcc gccgacccga
     4741 cggctcgagc ctcagctggg tcacctacgg gtttgcgaag ctggatcatc cgttggcgcc
     4801 gtttttcatc gagtggggcg atgcgcgcct gcatccgtcg cggacctcgc cggaaggctg
     4861 ctcgctgcgg tccctccgga tcgaggaccc ggccgccgac cgtctccgcc gcgcca
//
LOCUS       DASTZO010000124         3501 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1237963,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000124 DASTZO010000000
VERSION     DASTZO010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3501)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3501)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3501
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237963"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..304
                     /locus_tag="VFU20_03505"
     CDS             <1..304
                     /locus_tag="VFU20_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU5285564.1"
                     /translation="KALLKVVSHTPVEVEALNRLRQAHDWVDIGRKFMPAPERCFTWW
                     SYRSMDWTKNDRGRRLDHMWASPQLGGQVVAHHVLEPCRSWQRPSDHVPLICEVAL"
     gene            301..1353
                     /gene="ribA"
                     /locus_tag="VFU20_03510"
     CDS             301..1353
                     /gene="ribA"
                     /locus_tag="VFU20_03510"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HEU5285565.1"
                     /translation="MSEGRIRRAIAALKTGRPVRVERGRESIAVLPVETATAELLELL
                     DPGSRAPLLLSGARAVALALANERDAADPARPVLVERCAWLDCESALALADPGQDLAR
                     APIGPLQPAPLAHEALAAAALELARAAGLLPALWLLDPAMKAAAAVAVDDLTGIASPV
                     ALVARARLPLEDMPDTQIVAFRSPDGEEHVALVIGAFGGAPPLVRLHSECLTGDVFGS
                     LKCDCGPQLREALRLIGAAGGGVLLYLRQEGRGIGLANKLRAYALQDRGLDTVEANQR
                     LGFADDERDYDHAAAMLRALGIEEVRLLTNNAAKVAGLKAAGIAVVERVAHHMPANPH
                     NADYLAVKRAKSGHLA"
     gene            complement(1350..2003)
                     /locus_tag="VFU20_03515"
     CDS             complement(1350..2003)
                     /locus_tag="VFU20_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU5285566.1"
                     /translation="MNSAADRRQAILDDVAAAAKLARRDPGDVTLIAVSKTRSAEDIE
                     ALIAAGQRDFGESRVQEALAKWPPLLERHPDVTLHCVGRLQSNKAADAVALFGAIHSL
                     DRASLLAALVREAERQGRRPQVYVQVNIGEEVQKGGCAIGAVGDLVAAVRSSPLPLAG
                     LMAIPPLGVEPAPYFALLAELARRHDVAGLSMGMSADFKAAVMLGATAVRVGTALFD"
     gene            complement(2023..2676)
                     /locus_tag="VFU20_03520"
     CDS             complement(2023..2676)
                     /locus_tag="VFU20_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEU5285567.1"
                     /translation="MLTPALAAPQSKKRPPAVAVSFDPVATFTPATADPRLAAAFAGK
                     SASLTDFKFTPAPAKGRPSQVRVAIRARADAPGKAAEAAALASSVSALTPASYNLGVA
                     VGWRRFAVAGDVGKSKEANPALGGRESAALGVSYSLNKRLTGRVAVGADRGDGRALTP
                     LRKADNYSLDVGGAYSITRNIAVTGGVRYRIDHERLSALKDDRRDSQAVYVGTAFKF"
     gene            2928..3362
                     /locus_tag="VFU20_03525"
     CDS             2928..3362
                     /locus_tag="VFU20_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3576 domain-containing protein"
                     /protein_id="HEU5285568.1"
                     /translation="MPRLPTIAAALTVLALVGSGCSRNRDIPLQLAPSRTTSIGVNAY
                     LWRAAIDTVSFAPLLQADSNGGVIVTDWYTNPRSPGERVKLTVAILDQDLRADALRVA
                     ASRQVLQNGVWVDAPVAAATVQKLEDIILTRARDLRRTAVGG"
BASE COUNT          499 a         1255 c         1267 g          480 t
ORIGIN      
        1 caaggcgctg ctcaaggtcg tcagccacac cccggtcgag gtcgaggcgc tcaaccggct
       61 ccgccaggcg cacgactggg tcgacatcgg ccgcaagttc atgccggcgc cggagcgctg
      121 cttcacctgg tggtcgtatc gctcgatgga ctggaccaag aacgaccgcg gccgccgcct
      181 cgaccatatg tgggcgtcgc ctcagctcgg cggccaggtg gtcgcgcacc atgtgctcga
      241 gccgtgccgg agctggcagc ggccgtccga ccatgtgccg ctgatctgcg aagtcgcgct
      301 ttgagcgaag gccgcatccg gcgggcgatc gcggcgctca agaccggccg gccggtccgg
      361 gtcgagcgcg gacgggagag catcgcggtc ctgccggtcg agaccgcgac cgccgaattg
      421 ctcgagcttc tcgaccccgg ttccagggcg ccgctgctgc tcagcggggc tcgcgccgtc
      481 gcgctggcgc tggccaacga gcgcgacgcc gccgatcccg cgcgcccggt gctggtcgag
      541 cgctgcgcgt ggctggactg cgaatccgcg ctggcgctcg ccgacccggg acaggacctg
      601 gcgcgggcgc cgatcgggcc gctgcagccg gcgccgctgg cgcacgaggc actggccgcg
      661 gcggcgctgg agctggcgag ggcggccggg ctgctgccgg cgctgtggct gctcgatccg
      721 gcgatgaagg cggcggcggc ggtcgcggtg gacgacctta ccggaatcgc ctcgccggtc
      781 gcgctggtcg cccgcgcccg gctgccgctc gaggacatgc ccgacaccca gatcgttgcc
      841 ttccgaagcc ccgacggcga ggagcatgtc gcgctggtga tcggcgcgtt cggcggagcg
      901 ccgccgctgg tccggctgca cagcgagtgc ctgaccggcg acgtgttcgg atcgctcaaa
      961 tgcgattgcg ggccgcagtt gcgcgaggcg ctgcggctga tcggggcggc cggcggcggg
     1021 gtgttgctct atttgcgcca ggagggccgc ggcatcggcc tcgccaacaa gctgcgcgcc
     1081 tatgcgctgc aggaccgcgg gctcgacacg gtcgaggcca accagcgcct cggtttcgcc
     1141 gacgacgagc gcgactacga ccatgccgcg gcgatgctgc gcgcgctcgg gatcgaggag
     1201 gtgcggctgc tgactaacaa tgcggccaag gtcgccggtc tgaaggcggc cgggatcgcg
     1261 gtcgtcgagc gggtcgcgca ccacatgccg gccaacccgc acaacgccga ctatctcgcg
     1321 gtcaagcggg ccaagagcgg gcatttggcc taatcgaaca gcgccgtgcc gacccgcacc
     1381 gcggtggcgc cgagcatcac cgcggccttg aaatcggccg acatgcccat gctgaggccc
     1441 gcgacgtcat gacgccgcgc gagctcggcg agcagcgcga aataaggcgc cggctcgacc
     1501 cccagcggcg ggatcgccat cagcccggcg agcggcagcg gcgatgagcg caccgcggcg
     1561 accaggtcgc cgaccgcgcc gatcgcgcag ccgcccttct gcacttcctc gccgatgttg
     1621 acctggacgt agacctgcgg gcggcggccc tgccgttccg cttcccggac cagcgcggcg
     1681 agcagcgagg cgcgatcgag cgaatggatg gctccgaaca aagcgaccgc gtcggccgcc
     1741 ttgttcgact gcagccggcc gacacagtgg agggtgacat cgggatgacg ctcgagcagc
     1801 ggcggccatt tggcgagcgc ctcctgcacc cggctttcgc cgaagtcgcg ctggcccgcg
     1861 gcgatcaggg cctcgatgtc ctcggcgctg cgggtcttgc tgaccgcgat cagggtcacg
     1921 tcgccgggat cgcgccgcgc cagcttcgcg gcggcggcga cgtcgtcgag gatggcctgg
     1981 cggcggtcgg cggcgctgtt catcgcgccg gactaggccg gatcagaact tgaaggcggt
     2041 gccgacgtag acggcctggc tgtcgcggcg gtcgtccttg agcgccgaca gccgctcgtg
     2101 gtcgatccgg tagcggaccc cgccggtcac ggcgatgttg cgcgtgatcg aataagcgcc
     2161 gccgacgtcg agcgaataat tgtcggcctt gcgcagcggc gtcagggcgc gcccgtcgcc
     2221 gcggtcggcg ccgaccgcga cccggccggt caggcgcttg ttgagcgagt agctgacccc
     2281 cagcgcggcg ctctcgcgcc cgccgagcgc cggattggct tccttggact tgccgacatc
     2341 gccggcgacc gcgaaccgcc gccagccgac cgcgactccg agattgtagc tggccggggt
     2401 cagggcgctg accgacgagg ccaaagcagc ggcctcggcg gccttgcccg gagcgtcggc
     2461 gcgggcgcgg atcgcgaccc ggacctgcga cggccgtccc ttggccgggg ccggcgtgaa
     2521 cttgaaatcg gtcagcgacg cggacttgcc ggcgaacgcg gcggcgagcc gcgggtcggc
     2581 ggtcgccgga gtgaaggtcg cgaccgggtc gaagctcacc gcaaccgccg gcgggcgctt
     2641 cttggactgg ggagcggcga gggccggagt cagcacgaga ccggccgcgg cgagcgccgc
     2701 aaccgtcctg ccaaaccgtg accgacccaa gctattcaca gtgaactccc tgttcacgac
     2761 tcgatatagc cctcgcaggc ccaaggttcc atgtccggag tcgattcaca agcggcctgt
     2821 ggccgataca ccacaacatc ggctcgatgc gggacacgct tgcggagcaa gggccagcat
     2881 ctataacgcc gggctgacct tcgcacccgc gctttcgagg aaccacgatg ccccgcctgc
     2941 ccaccatcgc cgccgccctg accgtccttg cgctcgtcgg ctccggctgc agccgcaacc
     3001 gcgacatccc gctgcagctg gcgccgtcgc gcaccacctc gatcggggtc aacgcctatc
     3061 tgtggcgggc ggcgatcgac accgtgtcgt tcgcgccgct gctgcaggcg gactcgaacg
     3121 gcggggtgat cgtcaccgac tggtatacca acccccggtc gcccggcgag cgggtcaagc
     3181 tgaccgtcgc catcctcgac caggacctgc gcgccgacgc gcttcgggtc gccgcgtcgc
     3241 gccaggtgct ccagaacggc gtctgggtcg acgccccggt cgcggccgcc accgtccaga
     3301 agctcgaaga catcatcctg acccgcgccc gcgaccttcg ccgcaccgcc gtcggcggct
     3361 aggcgggccc gtgcgatgac gggccgcttc gacccggcgg acgccgagcg gcgctggcag
     3421 gcgcggtggg aggaggcggg ctgcttcgcc gccgacagcc gaagcccgaa gcccaagacc
     3481 tatgtgctgg agatgttccc c
//
LOCUS       DASTZO010000125         5859 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1003013,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000125 DASTZO010000000
VERSION     DASTZO010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1003013"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1165)
                     /gene="glmS"
                     /locus_tag="VFU20_03530"
     CDS             complement(<1..1165)
                     /gene="glmS"
                     /locus_tag="VFU20_03530"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010414081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU5285569.1"
                     /translation="MCGIVGIAGKTEVAQRLFDGLKRLEYRGYDSAGICTINDGELKR
                     RRAEGKLDNLQRELEAEPLGGTIGIAHTRWATHGAPTVGNAHPHIAGPVALVHNGIIE
                     NFKPLRDELIADGREFASETDSEVVAHLVAREIERGSSPEQAVAAVLPRLHGAFAIAF
                     LFRDHPDLIVGARMGAPLTVGYGEGENYVGSDALALAPLTQRIAYLEEGDWVAARRDR
                     VEIFDRDNKPVEREIVESGASAARIDKGNYRHYMQKEIFEQPIVVAQTLQSYVRPFEG
                     EVALPDAGLDLAAVDRLTIVACGTSYYAGLVAKYWVEQFARVPVDIDVASEFRYRQPV
                     LEPGGLALFVSQSGETADTLAALRYVRAQGGRIVSVVNVETSTSARESDVTLPI"
     gene            complement(1165..2523)
                     /gene="glmU"
                     /locus_tag="VFU20_03535"
     CDS             complement(1165..2523)
                     /gene="glmU"
                     /locus_tag="VFU20_03535"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEU5285570.1"
                     /translation="MSQTPDFAVVILAAGQGTRMRSDMHKVLHPIAGRPLLLHLLDRV
                     DGLGAQKRVVVVGKGRDQVEEALDGRDVAVAHQAQQKGTAHAVQQAAAALAGYGGAVL
                     VLYADTPFVEAATLKRMLERLDGDDGPGIVVLASRPADPAAYGRIILGEGDRIAKMVE
                     FRDASEEERAVRLCNSGMMALRARDLFRWLDRVGNDNAAGEYYLPDVVNIAASEGREA
                     VVIEGDPYETAGVNSRAELAHLELEWQRRRREQALEEGATLIDPESVWFAYDTRLGRD
                     VTVEPHVVFGPRVEVADGASIKAFSHIEGAVIGARASVGPFARIRPGTRLGETSRVGN
                     FVELKQALIGKGAKVNHLSYVGDSVVGEGANIGAGTITCNYDGFDKHQTVIGDGAFIG
                     SNSALVAPVTIGEGAVVGAGSVITGDVEPHSLALERSKQKGIAGWAKRFRAAKAAKKA
                     AR"
     gene            complement(2655..3080)
                     /locus_tag="VFU20_03540"
     CDS             complement(2655..3080)
                     /locus_tag="VFU20_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU5285571.1"
                     /translation="MQTTSDNVQGEPIMDINMTPLIDVLLVLLIMFIITIPIQTHAVK
                     LDLPQNQANQPPPPVDPIKNKVVITEAGAILWNGTPVSQLQLRQYLDVSQQMNPVPEL
                     HLQPEPNARYEIVDQVLAITKQAHVEKMGFVGNEAYAEF"
     gene            complement(3107..3598)
                     /locus_tag="VFU20_03545"
     CDS             complement(3107..3598)
                     /locus_tag="VFU20_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU5285572.1"
                     /translation="MAVSVGPGADEDMEAMSDINVVPLVDVMLVMLIIFLIAIPVVLQ
                     TVPVKLPAVRFQPTTTKPENVNLSVRGGPGGTCEVYWNLTKVDSKELLDRAVKKLEDE
                     IERQGGVENITEENMPEAHIRGDINTPYKCIGGTIYNMQRAGFARVGFISEPPAGGVT
                     TRL"
     gene            complement(3679..4494)
                     /locus_tag="VFU20_03550"
     CDS             complement(3679..4494)
                     /locus_tag="VFU20_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU5285573.1"
                     /translation="MATPAPAGENPYGMMQALQEGGIIAWSVFAILVIMSLGTFYILF
                     TKLMQQQKIMSQGRKVRASFWNSPNLREAATKLEKRSAYRAIVDDALIAQEQHGKLTD
                     PIDQHDWMANSLARSQGSIGARLGEGLAFLATVGSTAPFIGLFGTVVGIYRALIKIGA
                     SGQASIDTVAGPVGEALIMTALGLVVAVPAVLAYNWLVRRNKSIMEDLAAFTNDLHGY
                     LMSNGQVKPAMAGAETRGTATARASAPPPRGTEPVRPGDTRTGAEPTTVGDRP"
     gene            complement(4536..5168)
                     /locus_tag="VFU20_03555"
     CDS             complement(4536..5168)
                     /locus_tag="VFU20_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU5285574.1"
                     /translation="MSSNRTAAIIIVALLHVVLGYALVTGLAYNVAKQAMEDLKTFDV
                     EEEPPPPEEPPPPPEQPDTPPPPQIVAPPPLVRMDTVAPPIISTPVAPPPVITPIARP
                     PAPPAPPPPPKKIEPQSVSGNLQGLIRDSDYPQAAIDNEEQGTVSVVLQIGTTGRVTG
                     CSVSGSSGSRTLDSTTCRILTSRAKFSPARDSSGNPTTGTFRQRITWRLE"
     gene            complement(5405..5824)
                     /locus_tag="VFU20_03560"
     CDS             complement(5405..5824)
                     /locus_tag="VFU20_03560"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HEU5285575.1"
                     /translation="MSADAFRDEPGLDGLVPDHLRVRSSRILLDSLEVQADIGFHDYE
                     IGRPQRLLVTVEIWLENLSPPDDDSPAEAWDYDYLRTEVQALASARRYNLQETLAHAI
                     YQRFAALRGVKALRVATSKPDIYAEARGVGVEIASFN"
BASE COUNT          857 a         1989 c         1977 g         1036 t
ORIGIN      
        1 agatcggcag ggtgacgtcg ctctcgcgcg cgctggtcga ggtctcgacg ttaacgaccg
       61 agacgatgcg gccgccctgc gcccggacgt agcgcagcgc ggcgagcgtg tcggcggtct
      121 cgccggactg gctgacgaac agcgccagcc cgcccggctc gagcaccggc tggcggtagc
      181 ggaactcgga cgcgacatcg atgtcgacgg gcacccgggc aaactgctcg acccaatatt
      241 tggcgaccag cccggcgtaa tacgacgtgc cgcaggcgac gatggtcagc cggtcgaccg
      301 cggcgaggtc gagcccggcg tcgggcagcg ccacctcgcc ctcgaacggc cggacgtagc
      361 tttgcagcgt ctgggcgacg acgatcggct gctcgaagat ctccttttgc atgtagtggc
      421 ggtaattgcc cttatcgatc cgcgccgccg acgccccgga ctcgacgatc tcgcgctcga
      481 caggcttgtt gtcgcggtcg aagatctcga cccggtcgcg gcgcgcggcg acccagtcgc
      541 cttcctcgag ataggcgatg cgctgggtca gcggcgccag cgccagcgcg tccgagccga
      601 cgtaattctc cccctcgccg tagccgacgg tcagcggcgc ccccatccgc gcaccgacga
      661 tcaggtcggg atggtcgcgg aacaggaagg cgatcgcgaa tgccccgtgc aatcgcggca
      721 gcaccgccgc caccgcctgc tcgggcgacg agccgcgctc gatctcccgc gcgaccagat
      781 gcgcgacaac ttcgctgtcg gtctcgctcg cgaattcgcg gccgtcggcg atcaattcgt
      841 cgcgcagcgg cttgaaattc tcgataatcc cgttgtggac cagcgccacc ggcccggcga
      901 tgtgcgggtg cgcgttgccg accgtcgggg cgccgtgggt cgcccagcgc gtatgggcga
      961 tgccgatggt cccgcccagc ggctcggcct cgagctcgcg ctgcaaattg tcgagcttgc
     1021 cctcggcccg ccgccgcttc agctcgccgt cgttgatggt gcagattccg gccgaatcgt
     1081 agccgcggta ctcgagccgc ttcaggccgt cgaacagccg ctgggcgacc tcggttttgc
     1141 ccgcgatccc gacgatcccg cacattatcg agcggctttc ttcgcggcct tggcggcgcg
     1201 gaagcgcttc gcccagccgg cgatgccctt ctgcttgctg cgctcgagcg cgaggctgtg
     1261 cggctcgacg tcgccggtga tcaccgagcc cgccccgacc accgcgccct cgccgatggt
     1321 caccggagcg accagggcgc tgttggagcc gatgaaggcg ccgtcgccga tcaccgtctg
     1381 gtgcttgtcg aagccgtcgt aattgcaggt aatggtgccg gcgccgatgt tggccccctc
     1441 gccgacgacg ctgtcgccga catagctcag atggttgacc ttcgcgccct tgccgatgag
     1501 cgcctgcttc aactcgacga aattgccgac ccgcgaggtt tcaccgagcc gcgtccccgg
     1561 ccggatccgg gcgaacggcc caacgctcgc gcgggcgcca atcaccgccc cttcgatatg
     1621 gctgaacgcc ttgatgctcg ccccgtcggc gacctcgacc cgcggcccga acaccacgtg
     1681 cggctcgacc gtcacgtcgc ggccgagccg ggtgtcgtag gcgaaccaga cgctttccgg
     1741 gtcgatcagg gtcgcccctt cctcgagcgc ctgctcgcgc cggcgccgct gccattcgag
     1801 ctcgaggtgg gcgagctcgg cgcggctgtt gacccccgcg gtttcgtacg ggtcgccctc
     1861 gatgaccacc gcctcgcgcc cctcggaggc ggcgatgttg accacgtccg gcaggtaata
     1921 ttcgcccgcc gcattgtcgt tgccgaccct gtcgagccag cggaacaggt cgcgcgcacg
     1981 cagcgccatc atgccgctgt tgcacagccg gacggcgcgc tcctcctcgc tcgcgtcgcg
     2041 aaattcgacc atcttggcga tgcggtcgcc ctcgcccagg atgatccggc cgtaggctgc
     2101 gggatcggcg gggcgcgagg cgagaacgac gattccggga ccgtcgtcgc cgtcgagcct
     2161 ctcgagcatc cgcttcaggg tcgcggcctc gacgaacggc gtgtcggcgt agaggaccag
     2221 caccgcgccg ccatagccgg cgagcgccgc cgcagcctgc tggaccgcgt gggcggtgcc
     2281 cttctgctgc gcctggtggg cgaccgccac gtcgcggccg tcgagggcct cctcgacctg
     2341 gtcgcggccc ttgccgacga ccaccacgcg tttctgcgcg cccaatccgt cgacgcggtc
     2401 gagcaggtgc agcagcagcg gccggccggc gatcgggtgg agcaccttgt gcatgtcgga
     2461 gcgcattcgg gtgccctgcc cggcggcgag gatcacgact gcgaaatcgg gggtctggct
     2521 cacccgcggc ccaatggcac gaggattttc caagcgccag aggctgccgg ccctcgagcc
     2581 gatcgcccag ccggccaatg cgaacagggc ggcccgcgag gaccgcccct tctaatcacg
     2641 gcggccagga ccgcctagaa ctccgcgtag gcctcgttgc cgacgaagcc catcttctcg
     2701 acgtgggcct gcttggtgat cgccagcacc tggtcgacga tctcgtagcg cgcgttgggc
     2761 tcgggctgca ggtgcagctc ggggaccggg ttcatctgct gcgacacgtc gagatactgg
     2821 cgcagctgca gctggctgac cggcgtcccg ttccacagga tcgcgccggc ctcggtgatc
     2881 accaccttgt tcttgatcgg gtcgaccggc ggcggcggct ggttcgcctg gttctgcggc
     2941 agatcgagct tcaccgcgtg ggtctggatc gggatggtga tgatgaacat gatcagcagg
     3001 acgagcagga cgtcgatcag cggcgtcatg ttgatgtcca tgatcggctc gccctggaca
     3061 ttgtcgctgg tggtttgcat cgtcatggcg gcttgctcct atccagctac aggcgggtgg
     3121 tgacgccgcc tgccggcggc tcggagatga acccgacccg cgcaaagccg gcgcgctgca
     3181 tgttgtagat cgttccgccg atgcacttgt acggcgtgtt gatgtcgccg cggatgtgcg
     3241 cctcgggcat gttctcctcg gtgatgttct cgactccgcc ctggcgctcg atctcgtcct
     3301 cgagcttctt caccgctcgg tcgagcagct ccttgctgtc gaccttggta aggttccaat
     3361 agacctcgca ggtcccgccg ggaccgccgc ggacgctcaa attgacgttt tccggcttgg
     3421 tcgtggtcgg ctggaagcgg accgccggca gcttgaccgg gacggtctgc aacacgaccg
     3481 ggatggcgat caggaagatg atcagcatca ccagcatcac gtcgaccagc ggaacgacgt
     3541 tgatgtcgga catggcctcc atgtcttcgt ctgcgccggg acctacgcta actgccatcg
     3601 ccagtttcgc tttctgctgt ttgcatcggg gtcttcactg gaggggcgcc gacaccggcc
     3661 ggcgccctgc cttctcgatt acgggcggtc gccgacggtc gtcggttcgg cacccgtgcg
     3721 ggtgtcgccg ggacgcaccg gctcggtccc gcgcggcggc ggagcgcttg cccgcgccgt
     3781 cgcggttccg cgcgtttcgg cgccggccat cgcgggcttc acctggccgt tcgacatcag
     3841 gtagccgtgc aggtcgttgg tgaaggccgc gaggtcctcc atgatcgact tgttgcggcg
     3901 gaccagccag ttgtaggcga gcaccgcggg caccgcgacg acgaggccga gcgcggtcat
     3961 gatcagcgcc tcgccgaccg gcccggcgac ggtgtcgatc gacgcctggc ccgacgcgcc
     4021 gatcttgatc agcgcgcggt agatgccgac gacggttccg aacaggccga tgaacggcgc
     4081 ggtcgaaccg accgtcgcga ggaaggcgag gccttcgccg agccgcgctc cgatcgagcc
     4141 ctggctgcgc gccaggctgt tggccatcca gtcgtgctgg tcgatcgggt cggtcagctt
     4201 gccgtgctgc tcctgcgcga tgagcgcgtc atcgacgatc gcccgatagg cgctgcgctt
     4261 ctcgagcttg gtcgcggctt cgcgcagatt gggcgagttc cagaagcttg cccgcacctt
     4321 gcggccctgg ctcatgatct tctgctgctg catcagcttg gtgaacagga tgtaaaatgt
     4381 tcccagggac atgattacga gaattgcgaa aaccgaccag gcgatgatgc cgccctcctg
     4441 gagcgcctgc atcatgccat aagggttttc gcctgcgggt gctggtgttg ccatgatcgt
     4501 ctactttcct ctgatgattg cgccaagcat gggggctatt ccagccgcca cgtgatccgt
     4561 tgcctgaatg tcccggtggt cgggttgccg ctgctgtccc gggccgggga gaatttcgcc
     4621 cggctggtca ggattcggca ggtcgtgctg tcgagcgtcc tcgagccgct cgagccgctc
     4681 acgctgcagc ccgtcacccg gccggtggtg ccgatctgga ggacgaccga caccgtccct
     4741 tgctcctcat tgtcgatcgc cgcttgcggg taatcggagt cgcggatcag gccctgcaga
     4801 ttgccggaca cgctctgcgg ctcgatcttc ttgggcgggg gcggcggggc cggcggcgcc
     4861 ggcggccggg cgatcggggt gatcaccggc ggcggggcga ccggggtcga aatgatcggg
     4921 ggcgccaccg tatccatccg caccagcggc ggcggagcga cgatttgcgg cggcggcggg
     4981 gtgtccggct gttcgggcgg cgggggcggc tcctcggggg gcggcggctc ttcctcgacg
     5041 tcgaacgtct tcagatcctc catcgcctgc ttggcgacat tgtaggcgag gcccgtgacc
     5101 agggcatagc cgagcacaac gtggagcagc gcgacgatga tgatcgccgc cgtgcggttg
     5161 ctgctcatct gcttccgttg agcgtaggac attaagccgc aattctcctc gacacgccgc
     5221 tcgtgaaagc ggtcagacgg acgttccgga cgcgtgcccc gcccgggggg acggaagctg
     5281 ccgatgtatc aacatatcat cggccacaag cagcgtcctt ataacccgct caaaggccgc
     5341 gcaatcgatt ttgttccaca accgcaaggt gcgccgcaaa acgccggacg aagattcatc
     5401 cgattcaatt gaacgaggcg atttcgaccc caactccgcg cgcttccgcg tagatgtcgg
     5461 gcttggaggt cgccacccgg agcgccttga cgccgcgcag cgcggcgaaa cgctggtaga
     5521 tcgcgtgggc cagggtttcc tgcagattgt agcgccgggc gcttgccagc gcctggacct
     5581 cggtgcgcag ataatcatag tcccacgcct cggccggcga gtcgtcgtcc gggggcgaaa
     5641 gattttcgag ccagatctcg acggtcacca gcagccgctg cggccgcccg atctcatagt
     5701 cgtggaagcc gatgtcggcc tggacctcga gcgaatccag caggatccgg ctcgatcgca
     5761 cccgcagatg gtcgggcacg aggccgtcga gcccgggttc gtcgcgaaag gcgtcggcgc
     5821 tcattttgcc tccaggaact ggacatcgcg ctccagcga
//
LOCUS       DASTZO010000126         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1081124,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000126 DASTZO010000000
VERSION     DASTZO010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081124"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..1028)
                     /gene="dapD"
                     /locus_tag="VFU20_03565"
     CDS             complement(204..1028)
                     /gene="dapD"
                     /locus_tag="VFU20_03565"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HEU5285576.1"
                     /translation="MTTGIEAAIDAAWEDRDSIGPSTRGEARSAVDAALSALDSGTAR
                     IAEKTGGEWVVHQWLKKAVLLSFRLNPMEMIPGGPGGAHWWDKVPSKFAGWSEKQFET
                     AGFRAVPGAIVRRGAYIAPGAVLMPSFVNIGARVGEGTMVDTWATVGSCAQVGRNVHI
                     SGGTGIGGVLEPLQAGPVIIEDDCFIGARSEVAEGVVVEQGAVLSMGVFLGASTKIVD
                     RASGEIRYGRVPAYSVVVPGTLPGKDGGPGLACAVIVKRVDERTRSKTSINELLRD"
     gene            complement(1025..1708)
                     /locus_tag="VFU20_03570"
     CDS             complement(1025..1708)
                     /locus_tag="VFU20_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HEU5285577.1"
                     /translation="MDPSVAPAFRHVRDWIFDLDNCLYPASTGLFALIDERMGAYIQR
                     LLDCDANEAKRVQKAHFHEHGTTLAGLMKTHGVDPRDFLDEVHAIPLDRVRCDERLAA
                     ALERLPGRRFVFTNGDAPYARRVLDAIGVGSHFDELHDIHASELRPKPDPHGYALLCE
                     RFAIDPGRALLADDMAANLKPAKALGMTTVWVDNGSERGSHGADESFIDHRISDVAEW
                     LESILEDHA"
     gene            1824..>2738
                     /locus_tag="VFU20_03575"
     CDS             1824..>2738
                     /locus_tag="VFU20_03575"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5285578.1"
                     /translation="MSKRLFATTACAVVLAFGLSACGGGGGVDSTPTPTPTPTPTPTP
                     TPTPTPTPTPTPTPTPTNYDTTEYRASNYSVAANALAAYQAGATGQGIKIGVVDSGIN
                     PALAEFAGKVDPASRDVAGNRGVSDEDGHGTAVSAVAAAARNNLNTMGVAFDATIISL
                     RADTPGTCADTSEDGGCEFSDTAIAAGIDAARVAGARVINLSLGGSAPNSTLMAAMNR
                     AVSAGIVLVISAGNDGEKPEGANPDSFALVPAQNLGPSIIIAGSIGVADGSGGTDLNQ
                     LSTFSNKAGTGAAWYLTALGYRDRAPDHT"
BASE COUNT          446 a          994 c          896 g          402 t
ORIGIN      
        1 agcgcggcca tgatcgccca gtcgcggcgg ttcatcacgc tccggatcat cggcgccgca
       61 taagccgcgg cgccgcgcgc cgcgtcccgc ggcttgccgt caaaacaatc gtcgcggagc
      121 gtcgccatgc ccgccttgtg gccgcccgac gctccggcgg ccaaccaaag caatggggcc
      181 ggcgataagc tgggcttatg gggtcagtcg cgcagcagct cgttgatgct ggtcttggac
      241 cgggtgcgct cgtcgaccct cttgacgatc accgcgcagg cgaggcccgg gccgccgtct
      301 ttgccgggaa gagtcccggg cactaccacc gaataggccg gcacccggcc gtagcgaatc
      361 tcgcccgacg cccggtcaac gatcttggtc gaggcgccga ggaacacccc catcgacagc
      421 accgcgccct gctcgacgac caccccttcg gccacctcgg accgggcgcc gatgaagcaa
      481 tcgtcctcga tgatcaccgg cccggcctgc agcggctcga gcaccccgcc gatcccggtc
      541 ccgcccgaga tgtggacgtt gcggccgacc tgggcgcagc tgccgaccgt cgcccaggtg
      601 tcgaccatcg tcccttcgcc gacccgcgcg ccgatgttga cgaagctcgg catcagcacc
      661 gctccgggcg cgatataggc gccgcggcga acaatcgcgc ccggtacggc gcgaaatccc
      721 gccgtttcga actgcttttc gctccagccg gcgaatttgg acggcacctt gtcccaccag
      781 tgcgcgcccc ccggcccgcc ggggatcatc tccatcggat tgagccggaa cgacagcagc
      841 accgctttct tcagccactg gtggacgacc cactcgccgc ccgtcttctc ggcgatccgg
      901 gcggtcccgg aatcgagcgc cgacagcgcc gcgtcgaccg cgctgcgggc ttcgccgcgg
      961 gtcgacgggc cgatcgaatc gcggtcctcc caagcggcgt cgatggcggc ttcgatgccg
     1021 gtggtcatgc gtggtcctcc aggatcgatt cgagccactc ggcgacgtcg gaaatgcggt
     1081 ggtcgatgaa gctttcatcg gcgccgtggc tgccgcgctc ggagccgttg tcgacccaca
     1141 cggtggtcat tccgagcgcc ttggcgggct tcagattggc cgccatgtcg tcggccagca
     1201 gcgctcggcc aggatcgatc gcgaagcgct cgcacagcaa ggcatagccg tgcgggtcgg
     1261 gcttgggcct caattcgctg gcgtggatgt cgtgcagctc gtcgaagtgc gagccgacgc
     1321 cgatcgcgtc gagcacccgg cgggcgtagg gcgcatcgcc gttggtgaag acgaaccgcc
     1381 gccccggcag ccgctccagg gccgcggcga gccgctcgtc gcaccggacc cggtcgagcg
     1441 gaatcgcatg gacctcgtcg aggaagtcgc gcgggtcgac cccgtgggtc ttcatcaggc
     1501 cggccagggt cgtcccatgc tcgtggaaat gggccttctg gacgcgcttc gcctcattgg
     1561 cgtcgcagtc gagcagccgc tggatgtagg cgcccatgcg ctcgtcgatc agcgcgaaca
     1621 gccccgtcga ggccgggtaa aggcagttgt cgaggtcgaa aatccagtcg cggacatggc
     1681 gaaaggcggg tgcgacgctc ggatccatga tatggcaggc gcttacagag tctcgctgct
     1741 tcagcaaatt gaaaggattg gtaaagcata ggcccatacg gggaagccgt ggattttcag
     1801 cggcttgagc ggagataggg aaaatgagca aacggctttt cgcaacgacc gcctgcgcgg
     1861 ttgtgctggc ctttggactc tccgcctgcg gcggcggcgg cggtgtcgac tcgaccccga
     1921 ccccgacccc gaccccgacc ccgactccaa cccccacccc gacgcccaca ccgaccccga
     1981 cgcccacacc gaccccgacg ccgaccaact acgacacgac cgagtatcgg gcgtcgaact
     2041 attcggtcgc cgccaacgcg ctcgccgcct accaggccgg agcgaccggc cagggcatca
     2101 agatcggagt cgtcgacagc ggcatcaacc cggccctcgc cgagttcgcc ggcaaggtcg
     2161 acccggccag ccgcgacgtc gccggcaacc gcggggtcag cgacgaggac gggcacggga
     2221 ccgcggtgag cgcggtcgcc gccgccgccc gcaacaattt gaacaccatg ggcgtcgcct
     2281 tcgacgcgac gatcatcagc ctgcgcgccg acacgccggg gacctgcgcc gacacgtccg
     2341 aggacggcgg ctgcgagttc agcgacaccg cgatcgccgc cggcatcgac gccgcccgcg
     2401 tagccggcgc ccgggtgatc aacctgtcgc tcggcgggag cgcgcccaac tcgaccctga
     2461 tggcggcgat gaaccgcgcc gtctcggccg gcatcgtgct ggtgatttcc gccggcaacg
     2521 acggcgagaa gccggaaggc gccaaccccg attcgttcgc gctggtcccg gcccagaatc
     2581 tcgggcccag catcatcatc gccggctcga tcggcgtcgc ggacggcagc ggcggaaccg
     2641 atctcaacca gctttcgacg ttttccaaca aggccggcac cggcgcggcc tggtatttga
     2701 ccgcgctcgg ctatcgcgac cgagcccccg accatacc
//
LOCUS       DASTZO010000127         8156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_454957,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000127 DASTZO010000000
VERSION     DASTZO010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_454957"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="VFU20_03580"
     CDS             <1..792
                     /locus_tag="VFU20_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285579.1"
                     /translation="PTLAADQLEPEEPADFVPYAREPAQADEPTQPDEPSPAWDDGPS
                     GDFAEDDGAIDGAAAAIAVPEIVLGDDAGLADWLCAARETAEEVKSADGRSRAALYRA
                     LSRAYDFAVAAQRDPDDYAELLEESGVKAQARAPMTPIVKLVFGIDYDKTRLTEFASA
                     LSWAQRHQVELGGFEKLIEKQPGGLKALVAAERQARRPQAGPDTRAEAARERLRSAEP
                     LALQSLPGDEEFCLVLTRRGADGTHQPVARIADPALVERAIRRSA"
     gene            885..5531
                     /locus_tag="VFU20_03585"
     CDS             885..5531
                     /locus_tag="VFU20_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase domain-containing
                     protein"
                     /protein_id="HEU5285580.1"
                     /translation="MTVHTRVAKPLLDSLRKALTTHALPGETKGFTRECEREAAEFLA
                     GVAVRRRKGEVALAIESTGGEAGRRRMRIGIVNDDMPFLVDSVANAIAGRQLTIHRLL
                     HPVLCVTRDPSGRLREVEPLCGDESRQESMMYIELDRADARGRQELARELRAVLVDVR
                     AAVRDWRALQSKMRADSKQIPDWEGSALLDWFADGAMTLLGYQVEKPGEPPSQTLGIF
                     SIPGPPTDDAGCLGAMRYFERGGDIPLLAKAERKSTVHRRVPLDLVVVPVRERGKITG
                     IGVHAGLWTSQALTLPAEEVPVLRRRLDQLDEDFGFDPKGHSGKALRHAVASLPRDLL
                     INLSLDSMRELVMMAMSLADRPRPALLQVRSILKGHLFSFVWLPREELTTSRRTAIAM
                     MLEMEVGSEVSSWSVELGDGDLALIRYTQYVEEDAQLPDAAALDAALVEMVRGWAPAV
                     EAELIAAVGPARATRLSLTYLPAFSDSYRARTSPEDGAADILRLSALEDDVGRGVRIW
                     RAELDPPGQLHLKTYRKGGLIPLSEAVPVLENFGFRVLEEMPTALGGGALGYIHDFRL
                     EIGSEANLDSILARVAEIETAIACVLSGASEDDEFNQLTLFAGLDARPVQWLRAWFRY
                     LRQTGSAFGLVTVVDALRRAPKATAALIGLFIARHDPALGARRDAQVARQRAQFDDAL
                     ARVRSIDDDRILRRLRSLVEATVRTNAFSDAAREAVAFKIDSAMVPGLPAPVPWREIW
                     VYSPRVEGIHLRGGPVARGGIRWSDRRDDFRTEVLGLMKAQMVKNAVIVPTGAKGGFY
                     PKQLPPVTNRDAWLAEGTESYRIFIRSCLSITDNLVNDKAVHPANVVIHDGEDPYFVV
                     AADKGTAAFSDVANAIALERNFWLGDAFASGGSNGYDHKAMGITARGAWISVRRHFLE
                     LGIDVQTEPVTAVGCGDMSGDVFGNGMLLSKAVRLVAAFDHRHIFIDPNPDPVKSWKE
                     RKRLFGLPRSSWDDYDRSLLSTGGGIYPRTQKSISLSKQEREALGIDAETIDPAGLIN
                     AILRSPVDLIWFGGIGTYVKASTQLHAEVGDPANDALRADAADLRAKVIGEGANLAIT
                     QAGRIEFADRGGRINTDFIDNSAGVDCSDNEVNIKIPLNREMREGRLTLAKRNSLLAR
                     MTDEVAEIVLEDNRLQSLALSIAESSGAVAVPGHVRTIELLEAAGRLDRKVEGLAGSE
                     ELLRRVQEGRGLMRPELAVVLSMSKIVLQDAAEPLRLADDKMLEPQLFAAFPKPMRRA
                     HAEAIRAHRLRHEILGTKAANRFVNRLGPSVALDLTEEEGASLGQVIAAFLVAERLLD
                     LDELWAEIDQAELPEAVRIELFSIAASSVRSHLSDILRSAGGETRVSALCKLYESGVR
                     RIASAATKLIRTEVRNEAATRRDRLIALGASEQIVRGLVRLFELDGVFGLAALAARRK
                     VDEVALTHAYTKLGEALGLDWAQQQVARFVPGNQWERLLTAGLARDFEQLRIDFLSRR
                     RTSHPDAAVDQWVSSQGARIAQFRQLLNRARAEGAVSAPMLAQIASQARILLAR"
     gene            complement(5538..5759)
                     /locus_tag="VFU20_03590"
     CDS             complement(5538..5759)
                     /locus_tag="VFU20_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HEU5285581.1"
                     /translation="MGPIKRSVMIAGHPTSVSLEPIFWRALEVAARDLERPVNALVAE
                     IDAARLEVAPTPNLTSAIRQWLFGRALGG"
     gene            complement(5782..6504)
                     /gene="phbB"
                     /locus_tag="VFU20_03595"
     CDS             complement(5782..6504)
                     /gene="phbB"
                     /locus_tag="VFU20_03595"
                     /EC_number="1.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018454 - acetoacetyl-CoA reductase
                     activity [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetyl-CoA reductase"
                     /protein_id="HEU5285582.1"
                     /translation="MARVAIVTGGTRGIGEAISIALNGMGMKVAANYAGNDERARAFT
                     ERTGIRAYKWDVADYDACQDGVRQVEADLGPVDVLVNNAGITRDTTMKRMDRDKWQQV
                     IDTNLGGCFNMAKAVFEGMLGRGYGRIVNIGSINGQAGQYGQVNYAAAKSGIHGFTKA
                     LAQEGARSGVTVNAIAPGYIDTDMVAAVPEDVLAKIVARIPVNRLGKADEIARGVVFL
                     VDENAGFITGSTLSINGGQHMY"
     gene            complement(6537..7505)
                     /gene="hemH"
                     /locus_tag="VFU20_03600"
     CDS             complement(6537..7505)
                     /gene="hemH"
                     /locus_tag="VFU20_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU5285583.1"
                     /translation="MIGVLLVNLGTPEAPDAASVRAYLAEFLSDPRVIEIPQLLWQPI
                     LRGIILRTRPARSARAYREVWTDEGSPLLAIGMRQAAGLRDRLGDELIVGLAFRYGRR
                     SIGDAIARMVDAGVDRILFAPLYPQYCAATTASATDALSAQLESMRFQPAIRTLPPYF
                     DDPLYIGALRANLERQLGALAFEPQRLLLSFHGMPERTRQLGDPYHGQCLATARLLGD
                     ALGREVDVAFQSRFGRARWLEPATDATLAAYPGKGVERLAIAAPGFSADCLETLEELG
                     IRGRDSFLAAGGTHFARLDCLNDSPEGMAMLERLVRRELEGWRPAT"
     gene            complement(7502..>8156)
                     /locus_tag="VFU20_03605"
     CDS             complement(7502..>8156)
                     /locus_tag="VFU20_03605"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU5285584.1"
                     /translation="AVPAKSPFPAGYLRGHPQRAMAFFIESFIDELAHAAGLEPLVFR
                     MAMLGGSPRLARCIQAAAAAGGWDGGARGSSQGLAAVSAFGSHIGLLADATIGADQGI
                     EVHRLVAAVDCGRVVNPGLVRQQVESGLIHGLGLASVREPEWVAGMPRARAIGAIGLP
                     RIARTPDIRVHVVPSTAAPGGVSGLGVAALAPAVANAIHAATGKRLRSLPFDPMAAR"
BASE COUNT         1209 a         2901 c         2907 g         1139 t
ORIGIN      
        1 ccgaccctcg ccgcggacca gctcgagccc gaggagccgg ccgatttcgt gccctacgcg
       61 cgcgagccgg cccaggccga cgagccgacc cagcccgacg agccgagccc ggcctgggac
      121 gacggcccgt cgggcgactt cgccgaggac gacggtgcga tcgacggggc cgccgccgcc
      181 atcgccgtcc cggaaatcgt gctcggcgac gacgccgggc tcgccgactg gctgtgcgcc
      241 gcgcgcgaga ccgccgagga ggtcaagtcg gccgacggcc ggagccgggc cgcgctctac
      301 cgggcgctga gccgcgccta tgatttcgcc gtcgccgccc agcgcgaccc cgacgattat
      361 gccgagctgc tcgaggaatc gggcgtcaag gcgcaagccc gcgcgccgat gaccccgatc
      421 gtcaagctgg tgttcggcat cgactatgac aaaacccggc tgaccgagtt cgcgtcggcc
      481 ctgtcctggg cgcagcgcca ccaggtcgag ctcggcggct tcgagaaact gatcgagaag
      541 cagccgggcg ggctcaaggc gctggtcgcg gccgagcgcc aggcacggcg gccgcaagcc
      601 ggccccgaca cccgcgccga ggccgcccgc gagcggctgc gctcggccga gccgctggcg
      661 ctgcaatcgc tcccgggcga cgaggaattc tgcctcgtcc tgacccgccg cggcgccgac
      721 ggcacgcacc agccggtcgc ccggatcgcc gacccggcgc tggtcgagcg cgccatccgc
      781 cgctccgcct gacggccgac ttcctgtcga gccgggttgt cccggtcgcg gcggggcgtc
      841 tatagccgcg ccatcgcggc agtcggcgga gcgagctctt agaaatgacg gtccatacgc
      901 gcgtcgccaa gccgttgctg gactcgctgc gcaaggcgct gaccacccac gccttgccgg
      961 gcgaaaccaa gggcttcacc cgcgaatgcg agcgcgaggc ggccgaattc ctcgccgggg
     1021 tcgccgtgcg ccgccgcaag ggcgaggtcg cgctggcgat cgaatcgacc ggcggcgaag
     1081 ccggccggcg gcggatgcgg atcgggatcg tcaacgacga catgccgttc ctggtcgatt
     1141 cggtcgccaa cgccatcgcc ggccgccagc tgaccatcca ccgactgctt cacccggtgc
     1201 tgtgcgtgac ccgcgacccg agcggccggc tgcgcgaggt cgagccgctg tgcggcgacg
     1261 agtcgcgcca ggaatcgatg atgtacatcg aactcgacag ggccgacgcg cgcggccgcc
     1321 aggagctggc ccgggagctg cgcgcggtgc tcgtcgatgt ccgcgccgcg gtgcgcgact
     1381 ggcgcgcgct ccagtcgaag atgcgcgccg actccaagca aatccccgac tgggaagggt
     1441 cggcgctgct cgactggttc gccgacggcg ccatgacgct gctcggctac caggtcgaaa
     1501 aaccgggcga gccgccgtcg cagacgctcg gcatcttcag catcccggga ccgccgaccg
     1561 acgacgccgg ctgcctcggc gcgatgcgct atttcgagcg cggcggcgac atcccgctgc
     1621 tcgccaaggc ggagcgcaag tcgaccgtcc accgccgggt gccgctcgac ctggtcgtgg
     1681 tcccggtccg cgagcgcggc aagatcaccg gcatcggcgt ccacgccggc ctgtggacca
     1741 gccaggcgct gaccctgccg gccgaggaag tgccggtgct gcggcggcgg ctcgaccagc
     1801 tcgacgagga tttcggcttc gaccccaagg gccattcggg caaggcgctg cgccacgcgg
     1861 tcgcctcgct gccgcgcgac ctgctgatca acctcagcct cgattcgatg cgcgagctgg
     1921 tgatgatggc gatgtcgctc gccgaccggc cgcggccggc cctgctccag gtccgaagca
     1981 tcctcaaggg ccatctgttt tccttcgtct ggctgccgcg cgaggagctg accacctcgc
     2041 gccggaccgc gatcgcgatg atgctggaga tggaggtcgg ctcggaggtg agcagctggt
     2101 cggtcgagct cggcgacggc gacctcgcgc tgatccgcta cacccaatat gtcgaggagg
     2161 acgcgcagct gcccgacgcg gcggcgctcg acgcggcgct ggtcgagatg gtgcgcggct
     2221 gggcgccggc ggtcgaggcg gagctgatcg cggcggtcgg accggcgcgg gcgacccggc
     2281 tcagcctcac ctatttgccg gccttctccg acagctaccg ggcgcgcacc agccccgagg
     2341 acggggccgc cgacatcctt cggctgagcg cgctcgagga cgacgtcggg cgcggggtgc
     2401 ggatctggcg cgccgagctc gacccgccgg gccagctcca cctgaagacc tatcgcaagg
     2461 gcgggttgat cccgctgtcc gaggcggttc cggtgctcga gaatttcggc ttccgggtgc
     2521 tcgaggagat gccgaccgcg ctcggcggcg gagcgctcgg ctatatccac gatttccggc
     2581 tcgagatcgg cagcgaggcc aatctcgaca gcatcctcgc gcgggtcgcc gaaatcgaga
     2641 ctgcgatcgc ctgcgtgctc agcggtgctt cggaagacga cgagttcaac cagctgacgc
     2701 tgttcgcggg gctcgacgcg aggccggtgc aatggctgcg ggcctggttc cgctaccttc
     2761 gccagaccgg gagcgcgttc ggcctggtga cggtggtcga cgcgctgcgc cgggcgccca
     2821 aggccaccgc cgccctgatc gggctgttca tcgcccgcca cgaccccgcg ctcggcgcca
     2881 ggcgcgacgc gcaggtcgcc aggcaacgcg cccagttcga cgacgccctg gcgcgcgtcc
     2941 gctcgatcga cgacgaccgg atcctgcgcc gcctccgctc gctggtcgag gcgacggtgc
     3001 ggaccaacgc cttttccgac gcggcgcgcg aagcggtcgc cttcaagatc gactccgcga
     3061 tggtcccggg cctgccggcg ccggtgccgt ggcgcgagat ctgggtctat tcgccgcgcg
     3121 tcgagggcat ccaccttcgc ggcgggccgg tcgcccgcgg cggaatccgc tggtcggacc
     3181 ggcgcgacga tttccgcacc gaggtgctgg gcctgatgaa ggcgcagatg gtcaagaacg
     3241 cggtcatcgt cccgaccggg gccaagggcg gcttctaccc caagcagctg ccgccggtga
     3301 cgaaccgcga cgcctggctc gccgagggca ccgagagcta ccggatcttc atccgctcgt
     3361 gcctgtcgat caccgacaac ctcgtcaacg acaaggcggt ccacccggcc aacgtcgtca
     3421 tccacgacgg cgaggacccc tatttcgtgg tcgccgcgga caagggcacg gcggcttttt
     3481 ccgacgtcgc caacgcgatc gcgctcgagc gcaacttctg gctcggcgac gccttcgcca
     3541 gcggcgggtc gaacggctac gaccacaagg cgatggggat caccgcccgg ggcgcgtgga
     3601 tctccgtccg gcgccacttc cttgagctcg ggatcgacgt gcaaaccgag ccggtgacgg
     3661 cggtcggctg cggcgacatg tcgggcgacg tgttcggcaa cggaatgctg ctgtcgaagg
     3721 cggttcggct ggtcgccgcg ttcgaccacc gccacatctt catcgacccc aatcccgacc
     3781 cggtgaagag ctggaaggag cgcaagcggc tgttcgggct gccgcgctcg agctgggacg
     3841 actacgaccg ctccttgctg tccaccggcg gcggcatcta cccgcgcacc cagaaatcga
     3901 tcagcctgtc gaagcaggag cgcgaggccc tcggcatcga tgccgagacc atcgacccgg
     3961 cgggcctgat caacgcgatc ctgaggagcc cggtcgacct gatctggttc ggcggcatcg
     4021 gcacctacgt caaggcctcg acccaattgc acgccgaggt cggcgacccg gccaacgacg
     4081 cgctccgggc cgatgccgcc gacctgcgcg ccaaggtcat cggcgagggc gccaacctgg
     4141 cgatcacgca ggccgggcgg atcgagttcg ccgaccgcgg cggccgcatc aacaccgact
     4201 tcatcgacaa cagcgccggg gtcgattgct cggacaatga ggtcaacatc aagatcccgc
     4261 tcaaccgcga gatgcgcgaa gggcggctga ccctggccaa gcgcaactcg ctgctggcgc
     4321 ggatgaccga cgaggtcgcc gagatcgtgc tcgaggacaa ccggctgcag agcctggcgc
     4381 tgtcgatcgc cgaaagcagc ggcgcggtcg cggtgccggg ccatgtccga accatcgagc
     4441 tgctcgaggc ggccgggcgg ctcgaccgca aggtcgaggg gctggcgggc agcgaggagc
     4501 tgctgcgccg ggtccaggaa ggccgcgggc tgatgcgccc cgagctggcg gtggtgctgt
     4561 cgatgtccaa gatcgtcctc caggacgccg ccgagccgct gcgcctcgcc gacgacaaga
     4621 tgctcgagcc gcaattgttc gcagccttcc ccaagccgat gcgcagggcc cacgccgagg
     4681 cgatccgggc gcatcgcctg cgccacgaga tcctcggcac caaggccgcc aaccgcttcg
     4741 tcaaccgcct cgggccgagc gtcgcgctcg acctgaccga ggaggaaggc gcgtcgctcg
     4801 gccaggtgat cgccgccttc ctggtcgccg agcggctgct cgacctcgac gaactctggg
     4861 ccgagatcga ccaggcggag ctgcccgagg cggtgcgcat cgaactgttc tcgatcgccg
     4921 cgagcagcgt gcgctcgcat ctgtcggata tcctgcgctc ggcgggcggg gaaacccgcg
     4981 tcagcgcctt gtgcaagctg tacgaatccg gggtgcgcag gatcgcgtcc gcggcgacca
     5041 agctgatccg gaccgaggtc cgcaacgagg cggcgacccg ccgcgaccgg ctgatcgcgc
     5101 tcggggcgag cgagcagatc gtccgcggcc tggtccggct gttcgagctc gacggggtgt
     5161 tcgggctcgc cgccctggcc gcgcggcgca aggtcgacga agtggcgctg acccacgcct
     5221 acaccaagct cggcgaggca ttgggcctcg actgggcgca gcagcaggtc gcccgcttcg
     5281 tgcccggcaa ccagtgggag cggcttctga ccgccggcct ggcgcgcgat ttcgagcagc
     5341 ttcggatcga cttcctgtcg cgccgccgga ccagccaccc cgacgccgcg gtcgaccagt
     5401 gggtgtcgag ccagggcgcc cggatcgccc agttccgcca attgctcaac cgcgcccggg
     5461 ccgagggcgc ggtcagcgcg ccgatgctcg cccagatcgc cagccaggcg cggatcctgc
     5521 tcgcgcgctg agccggctca gccgcccagc gcgcgcccga acagccattg gcggatcgcg
     5581 gacgtcaggt tcggggtcgg agcgacctcg agccgggcgg cgtcgatttc ggcgaccagg
     5641 gcgttcaccg gccgctccag gtcgcgcgcc gcgacctcca gcgcgcgcca gaagataggc
     5701 tcgaggctga ccgaagtcgg atggccggcg atcatgactg agcgcttgat ggggcccata
     5761 gtccgctccg gcaccgcccc ctcaatacat atgctggccg ccgttgatcg acagggtcga
     5821 gccggtgatg aagccggcgt tctcgtcgac caggaagacg accccgcggg cgatctcgtc
     5881 ggccttgccg agccggttga ccgggatccg ggcgacgatc ttggcgagca cgtcctcggg
     5941 caccgctgcg accatgtcgg tgtcgatgta gcccggggcg atcgcgttga cggtgacccc
     6001 cgagcgcgcg ccttcctggg cgagcgcctt ggtgaagccg tggatgcccg acttggcggc
     6061 ggcgtaattg acctggccgt actggccggc ctggccgttg atcgagccga tgttgacgat
     6121 ccgcccgtag ccgcggccga gcatgccctc gaacaccgcc ttggccatgt tgaagcagcc
     6181 gccgaggttg gtgtcgatca cctgctgcca cttgtcgcgg tccatgcgct tcatggtcgt
     6241 gtcgcgggtg attccggcgt tgttgaccag cacgtcgacg ggcccgaggt cggcctcgac
     6301 ctggcggact ccatcctggc aggcgtcata gtcggcgacg tcccatttgt aggcgcggat
     6361 gccggtgcgc tcggtaaagg cgcgggcgcg ctcgtcgttg ccggcgtaat tcgccgcgac
     6421 cttcatcccc atgccgttga gagcgatgct gatcgcttcg ccgatgccgc gcgtgccgcc
     6481 ggtgacgatc gccactcgag ccattgatcc ctcctgatgc gtaaggccga cgcggcttag
     6541 gtcgccggcc gccaaccttc aagttcgcgc cgcaccagcc gctcgagcat cgccatgccc
     6601 tcgggcgaat cgttgaggca gtcgagccgt gcgaaatgcg ttccgccggc ggcgaggaac
     6661 gagtcgcggc cgcggatccc gagctcctcc agagtctcca ggcaatcggc ggaaaagccg
     6721 ggcgccgcga tcgcgagccg ctcgacgccc ttgccgggat aagcggcgag cgtcgcgtcg
     6781 gtcgccggct cgagccatct ggcgcggccg aagcgcgact ggaaggcgac atcgacctcg
     6841 cggcccaagg cgtcgccgag cagccgggcg gttgccaggc actggccgtg atagggatcg
     6901 ccgagctgcc gagtgcgctc cggcatgccg tggaagctca gcagcagccg ctgcggctcg
     6961 aaggcgagcg ctccgagctg ccgctcgaga ttggcgcgca gcgcgccgat gtagagcggg
     7021 tcgtcgaagt aaggcggcag ggtgcggatg gcgggctgga agcgcatgct ttcgagctgc
     7081 gcggacaagg catcggtggc gctcgccgtc gtcgccgcgc aatattgcgg gtagagcgga
     7141 gcgaacagga tccggtcgac accggcgtcg accatcctcg cgatcgcgtc gccgatcgat
     7201 cgccggccgt agcggaaggc gagcccgacg atcagctcgt cgcccagccg gtcgcgcagc
     7261 ccggccgcct ggcgcatgcc gatcgccagc agcggcgatc cctcgtcggt ccagacttcg
     7321 cggtaggcgc gcgccgaccg ggccgggcgg gtgcgcagga tgatcccgcg caggatcggc
     7381 tgccacagca attgcgggat ctcgatgacc cgcggatcgg acaggaattc ggcgagatag
     7441 gcgcgcaccg acgcggcgtc gggcgcttcg ggggtgccga ggttgacgag caggactccg
     7501 atcatcgggc cgccatcggg tcgaacggca ggcttcgcaa ccgctttccg gtcgcggcgt
     7561 ggatcgcgtt ggcgaccgcc ggagcgagcg cggcgacgcc gaggccgctg accccgccgg
     7621 gcgcagcggt gctgggcacg acgtgcaccc ggatgtcggg cgtccgggcg atgcgcggca
     7681 ggccgatcgc tccgatggca cgcgcgcgcg gcatccccgc cacccactcg ggttcgcgga
     7741 cgctggcgag gccgagcccg tggatgaggc cgctttcgac ctgctggcgg accagccccg
     7801 ggttgacgac ccggccgcaa tcgaccgcgg cgaccagccg atggacctca atgccctggt
     7861 cggcgccgat ggtcgcgtcg gcgagcagcc cgatgtgcga gccgaacgcc gagaccgcgg
     7921 cgaggccctg gctgctgccg cgcgcgccgc cgtcccagcc gcccgccgcc gcggccgcct
     7981 ggatgcagcg cgcgagccgc gggcttccac ccagcatcgc catccggaac actagcggct
     8041 cgaggccggc ggcgtgggcg agctcgtcga tgaagctttc gatgaaaaag gccatcgccc
     8101 gctgcgggtg gccgcgcaga tagccggccg ggaacggcga cttcgccggc accgcg
//
LOCUS       DASTZO010000128         5360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_246352,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000128 DASTZO010000000
VERSION     DASTZO010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246352"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="VFU20_03610"
     CDS             <1..327
                     /locus_tag="VFU20_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285585.1"
                     /translation="SAGLTGARREREDILCVNPLTGSQGGAAPPAANGGTLVPSPDLR
                     SATLVPGRVGARCGKGLLLLDGAIPLLGRYVLPGNNYHVYDYALFWDAVRADALRRTS
                     AWRASR"
     gene            324..797
                     /gene="ruvX"
                     /locus_tag="VFU20_03615"
     CDS             324..797
                     /gene="ruvX"
                     /locus_tag="VFU20_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU5285586.1"
                     /translation="MITTRPAEFAAALPGGGKLAGLDVGTRTIGLALCDSGWSFAGPA
                     ETIRRTKFAADLEALRGFISRERVAGLVVGLPLNLDGSDSPRTQSVRAFARNLAPLGL
                     PVLLWDERWSTQAVERAMIAADVSRARRAQAVDKLAAAHILQGAIDALANLPAPE"
     gene            809..1693
                     /locus_tag="VFU20_03620"
     CDS             809..1693
                     /locus_tag="VFU20_03620"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU5285587.1"
                     /translation="MDLLSIDSLSDDQIATILDEAGRWAESNRTDSSLAGRIVFNLFY
                     ENSTRTVMSFAVAAHRLGAQVVTLPVEHSSAKKGETLEDTARTLNAMRPDALVVRHFE
                     NGAPARMAQIMDCPVINAGDGTNEHPTQALLDALTIRRHFGRIAGLKVAICGDIKHSR
                     VARSNAKLLPRLGADVRLAGPPALLPEDLHGSGDVDSAIEGADVVMMLRIQRERLESD
                     LGDAPGEYLARYGLTPERLKRASPRAAVMHPGPMNRGVEIASAVADDPERSLIPLQVE
                     TGVAVRMACLRLLVGGPC"
     gene            complement(1707..3035)
                     /locus_tag="VFU20_03625"
     CDS             complement(1707..3035)
                     /locus_tag="VFU20_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5285588.1"
                     /translation="MSKPLRFGSALSAIALASAIAGCATPAGRSAAFFGGKDGKGDIG
                     LAMRALVALNANDYATAVSLAERAVEKSPNDAGFRALLGNAYFASGRFASAESAYRDS
                     LSLIANQPQVVLKLALVEIAQGRNAEALALLGEARGMLEPADHGLAVALAGQPQEAAQ
                     ALEAAARQPGADARLRQNLALAYALSGNWDAARTVAAQDLSADLLDGRIQQWMAFAKP
                     NAAADQVAALTGVRPVASDPGQPTRLALRGAATRTAEAAPAAEQPQVAAFAPQPVAGE
                     AEAPRFDQLAAAPVPEPAAPVVQAAAPAPAPAFVETAPVIEPAYVEPKRARVLSASFT
                     AKLKPARPASLNRRTGNSTAVVQLGAYGSPQRVAAAWNAASRRYSVLRGYSPVSARFN
                     SSKGLVYRLSVKGFGNPNEAKSLCVSVRRAGGSCFVRSVAGDVPVRLASR"
     gene            complement(3040..3738)
                     /locus_tag="VFU20_03630"
     CDS             complement(3040..3738)
                     /locus_tag="VFU20_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285589.1"
                     /translation="MKSEPKVFASLSSGLLARKGAARPAMRPQGFGQGGGALEDLGWD
                     DMGFEPPKPAAAPRDETHDAFGAELEEQPLRNPLAALTPVQSPVHSQQAEIAGRLQVP
                     DEELSEEGEEIDETAELYEPEADTAEAQTAVAEAPEARMPEPQDVPPVVVELTPARAP
                     VARRAPAPRSAPGSKAKAAFTLRLDPERHLKLRLACAVSGRSAQLLVTDALDTLLGSM
                     PELDAMALKANRKG"
     gene            complement(3751..4464)
                     /locus_tag="VFU20_03635"
     CDS             complement(3751..4464)
                     /locus_tag="VFU20_03635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU5285590.1"
                     /translation="MRVLAMASQKGGSGKTTLSGHLAVQAQMAGAGPVCLIDIDPQGS
                     LADWWNERQADMPAFAQTTVARLASDLEVLRQQGFRLAVIDTPPAITMAIQSVIAVAE
                     LIVIPTRPSPHDLRAVGATVDLCDRAGKPLIFVVNAATPKAKITYEAAVALSQHGTVA
                     PVTLHHRTDYAASMIDGRTVMEVDPGGRSAREVVELWDYISDRLEKNFRRTVFAAPGA
                     AAPISPRPVGGFGRRVAGQ"
     gene            4598..>5360
                     /locus_tag="VFU20_03640"
     CDS             4598..>5360
                     /locus_tag="VFU20_03640"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5285591.1"
                     /translation="MLNVALGALLLAAAVPGAAQSVKAGIEAWQKADYAAAVGIWRPL
                     AEAGDADASFNLGQAYRLGRGVPLSLAAAQTWFERAARKGHLDAQTTLGLLLFNGGNR
                     VSGLRWLKTAAEQGEPRALLIYGTALFNGDGVVRDPALAYAYVSRAAAQGLAPAKTTL
                     AEMDKLVPLEQRRKGIAMAQDMAKSAKAAEPPPAAKPRGGAVHVAEQPPAKAAARPPK
                     KRAAPVAAAAAGPWRVQLGAFSKRASAEALFDRLSG"
BASE COUNT          762 a         1890 c         1960 g          748 t
ORIGIN      
        1 tcggccggct tgacgggcgc ccggcgcgag cgcgaggaca tcttgtgcgt caacccgctg
       61 accggcagcc agggcggggc cgcgccgccc gcggccaatg gcgggacgct ggtcccgtcc
      121 cccgacctcc gttcggcgac actggtgccc gggcgggtcg gggcgcgctg cggcaagggg
      181 ctgttgctcc tggacggcgc gatcccgctg cttggccggt acgtgctgcc gggcaataat
      241 taccacgtgt acgactatgc cctgttctgg gatgcggtcc gcgccgatgc gctgcggcgg
      301 acaagcgcat ggcgggcaag ccgatgatca ccacccgccc ggccgagttc gcggcggcgc
      361 tgcccggcgg cggcaagctc gccgggctcg acgtcggcac ccggaccatc ggccttgcgc
      421 tgtgcgattc gggctggagc ttcgccggcc ccgccgagac catccgccgg acgaaattcg
      481 ccgccgacct cgaggccctg cgcgggttca tcagccgcga gcgggtcgcc ggcctggtcg
      541 tcggcctgcc gctcaacctc gacggcagcg atagcccgcg cacgcagtcg gtccgcgcct
      601 tcgcccgcaa cctcgcaccg ctcggcctgc ccgtcctgct gtgggacgaa cgctggtcga
      661 cccaggcggt cgagcgggcg atgatcgccg ccgacgtcag ccgcgcccgg cgcgcgcaag
      721 cggtcgacaa gctcgccgcc gcgcacatcc tccagggagc gatcgacgcg ctggccaacc
      781 tccccgcgcc ggaataagcg cttctttcgt ggacctgctc tcgatcgact ccctcagcga
      841 cgaccagatc gcgacgatcc tcgacgaagc cgggcgctgg gcggaatcga atcgcaccga
      901 ctcctcgctc gcgggccgga tcgtcttcaa cctgttctac gagaactcga cccggaccgt
      961 gatgtcgttc gcggtcgccg cgcaccggct cggcgcgcag gtcgtcactt tgccggtcga
     1021 gcattcgtcg gccaagaagg gcgagacgct cgaggacacc gcccggacgc tcaacgcgat
     1081 gcggcccgac gctttggtcg tccgccactt cgagaacggg gcgccggcgc ggatggcgca
     1141 gatcatggac tgcccggtga tcaacgccgg cgacggcacc aacgagcacc cgacccaggc
     1201 cctgctcgac gcgctgacca tccgccgcca cttcggccgg atcgccgggc tcaaggtcgc
     1261 gatctgcggc gacatcaagc acagccgggt cgcccggtcg aacgccaagc ttctgcccag
     1321 gctcggcgcg gatgtgcgcc tggccggccc gcctgccctg ctgcccgaag acctgcacgg
     1381 cagcggcgac gtcgattcgg ccatcgaggg cgccgacgtt gtgatgatgc tgcgaatcca
     1441 gcgcgagcgg ctcgagtcgg acctcggcga tgcccccggc gaatatctcg cgcgctacgg
     1501 cctgaccccc gagcggctga agcgggcgtc gccccgggcc gcagtcatgc acccgggccc
     1561 gatgaatcgt ggagtggaga tcgccagcgc cgtcgccgat gacccggagc ggtcgctgat
     1621 cccgcttcag gtcgaaaccg gggtcgcggt ccgcatggcc tgcctcaggc tgctggtcgg
     1681 cggcccttgc tgatcgtcag ccgcgcctag cgcgaggcga ggcggaccgg gacatcgccg
     1741 gcgaccgagc ggacgaagca gcttccgccg gcccggcgca ccgacacgca caggctcttg
     1801 gcctcattgg gattgccgaa ccccttgacc gacaggcgat agacgaggcc cttcgagctg
     1861 ttgaagcgcg cgctcaccgg cgaatagccg cgaagcaccg aatagcgccg cgaagcggcg
     1921 ttccacgcgg cggcgacgcg ctgcggcgag ccgtaggcgc cgagctgcac gaccgcggtc
     1981 gaattgccgg tgcggcggtt aagcgaggcc gggcgagccg gcttgagctt ggcggtaaag
     2041 ctggccgaga ggacccgcgc ccgcttcggc tcgacataag cgggctcgat caccggagca
     2101 gtctcgacga acgccggagc cggcgccggg gcggcggcct ggacgaccgg cgccgcgggc
     2161 tcgggaaccg gcgcggcggc cagctggtcg aacctgggcg cctcggcctc gccggcgacc
     2221 ggctgcggcg cgaaggcggc gacctgcggc tgctcggcgg ccggagcggc ctcggctgtg
     2281 cgggtcgctg cgccgcggag cgcgaggcgg gtcggctggc ccgggtcgct cgccaccggc
     2341 cggacgccgg tcagagcggc gacctggtcg gcggcggcat tgggcttggc gaaggccatc
     2401 cattgctgga tccggccatc gagcaggtcg gccgaaagat cctgggcggc gacggtccgg
     2461 gcggcgtccc agttcccgga cagcgcataa gccagagcga ggttctggcg cagtcgggca
     2521 tcggcgccgg gctggcgggc cgccgcttcg agcgcttgcg ccgcctcctg cggctggccg
     2581 gcgagcgcga cggcaaggcc gtggtcggcg ggctcgagca tgccgcgcgc ttcgcccagc
     2641 aaggccagcg cctcggcgtt gcggccctgc gcgatctcga ccagcgccag cttcaggacg
     2701 acctgcggct ggttggcgat cagcgacagc gagtcgcggt aggcggattc ggccgacgcg
     2761 aagcggcccg aagcgaaata ggcattgccg agcagcgccc ggaagccggc gtcgttgggg
     2821 cttttctcga ccgcccgctc ggcgaggctg accgccgtcg catagtcgtt ggcgttgagc
     2881 gcaaccagcg cccgcatcgc caggccgata tcgcccttgc cgtccttgcc gccgaaaaag
     2941 gcggcgctgc gtcccgccgg agtggcgcaa ccggcgatgg ccgaggccaa agcgattgcc
     3001 gagagtgcgg aaccgaagcg gagcggtttg gacatcagtt tatcccttgc ggttggcttt
     3061 cagggccatt gcgtcgagct ccggcatgga gccgagcagc gtgtcgagtg cgtcggtgac
     3121 gagcagctgc gccgagcggc cgctgacggc gcaggccagg cggagcttga ggtgacgctc
     3181 cgggtcgagg cgcagagtga acgccgcctt ggccttgctt cccggcgccg agcgcggagc
     3241 cggggcgcgg cgggcgaccg gcgcacgcgc cggggtcagc tcgaccacca ccggcggcac
     3301 atcctggggc tcgggcattc gggcctcggg ggcttcggcc accgcggttt gagcctcggc
     3361 ggtgtccgcc tcgggctcgt aaagctcggc ggtctcgtcg atctcttcgc cttcctcgga
     3421 aagctcctcg tccggcacct ggagccggcc ggcgatctcc gcctgctggc tgtggaccgg
     3481 cgactgcacc ggggtcagcg ccgcgagcgg gttgcgcagc ggctgctcct cgagctcggc
     3541 gccgaacgcg tcgtgagtct cgtcgcgcgg cgcggcggcc ggcttgggag gctcgaagcc
     3601 catgtcgtcc cagccgaggt cctcgagcgc gccgccgccc tggccgaagc cctggggccg
     3661 cattgccggg cgagcggcac ccttgcgggc caggaggccc gaagagagcg acgcgaaaac
     3721 cttgggttcg cttttcacct ggcggccccc ttactggccg gccactcggc ggccgaaacc
     3781 gccgaccggg cgaggtgaaa tcggggccgc tgccccggga gccgcaaaca cggtgcggcg
     3841 gaaattcttt tcgagccggt cggaaatata gtcccacaat tcgacgactt cacgcgccga
     3901 gcggccgccg gggtcgactt ccatgacggt tcgcccgtcg atcatcgacg cggcgtaatc
     3961 ggtgcggtga tggagcgtga ccggggcgac ggtgccgtgc tgcgacagag cgaccgcggc
     4021 ctcgtaggtg atcttggcct tgggggtcgc cgcgttgacg acgaagatca gcggcttgcc
     4081 ggcgcggtcg cacagatcga ccgtggcgcc gacggcgcga aggtcgtgcg ggctcggccg
     4141 ggtcgggatg acgatcagct cggcgacggc gatgacgctc tggatggcca tggtgatcgc
     4201 cggcggagtg tcgatcaccg cgaggcgaaa gccctgctgg cgaaggacct cgaggtcgga
     4261 cgcaagccgg gcgaccgtgg tttgagcgaa ggccggcatg tcggcctggc gctcgttcca
     4321 ccagtcggcg aggctgccct gcgggtcgat gtcgatcaga cagaccggcc ccgcgcccgc
     4381 catctgcgcc tgcaccgcaa gatgcccgga caaggtcgtt ttgcccgacc cgcccttctg
     4441 cgatgccatt gccagaacgc gcatgctttt cccccgccca gtgacctgcg ttgaacggcg
     4501 ggagtgacac tcaagcctct aaaatctggt taacagtggc acgaccgtgt tgccccccgg
     4561 gaacaggcgg ttacaagtgt gtccgatgcg acatgggatg ctcaacgtcg cgctcggcgc
     4621 gctgctgctg gccgcggccg tcccgggagc cgcgcagtcg gtcaaggccg gcatcgaggc
     4681 gtggcagaaa gccgattatg ccgcggcggt cggcatctgg cgcccgctgg ccgaggcggg
     4741 cgacgccgac gcctcgttca acctcggcca ggcctaccgg ctcggccgcg gagtcccgct
     4801 cagcctggcc gcggcgcaga cctggttcga gcgcgcggcg cgcaagggtc atctcgacgc
     4861 ccagaccacg ctcggcctgc tgctgttcaa cggcggcaac cgggtcagcg ggctgcgctg
     4921 gctgaaaacc gccgccgagc agggcgagcc gcgcgcgctg ctgatctacg gcaccgctct
     4981 gttcaacggc gatggggtcg tgcgcgaccc ggcgctcgct tacgcttatg tcagccgcgc
     5041 cgcggcgcag ggcctcgcgc cggccaagac gacgctggcc gagatggaca aattggtccc
     5101 gctcgagcag cgccggaagg gcatcgcgat ggcgcaggac atggccaagt ccgccaaggc
     5161 cgcggaaccg ccgccggctg caaaaccgcg cgggggcgcg gtgcacgtcg cggaacagcc
     5221 gccggccaag gccgcggcca ggcccccgaa gaagcgggcg gcccccgttg ccgcagcggc
     5281 ggccggcccg tggcgcgtcc agctcggggc cttttcgaag cgcgcctcgg ccgaagcgct
     5341 gttcgaccgg ctgtcgggca
//
LOCUS       DASTZO010000129         6780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1055786,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000129 DASTZO010000000
VERSION     DASTZO010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055786"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..735
                     /locus_tag="VFU20_03645"
     CDS             <1..735
                     /locus_tag="VFU20_03645"
                     /inference="COORDINATES: protein motif:HMM:NF018352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HEU5285592.1"
                     /translation="NWTVALGAAWIPDYVGSDDYRLIPAAAVRGKIGGIGITTRSTYL
                     YADLVPRGAGKVDFDAGPIIGARFNRTGKIKDDVVDLLPERKTAIEVGAFVGASLHGL
                     TNPYDSLSFRLDLLRDVGSAHESTVIAPDISFSTPLSRWTYVSASLGADFVANRFADY
                     YFSVSPAESVLTGLPAFNADGGMKSWKIGLLANQSVTGDLTGGLSLFGTVNYSRLVGD
                     FKRSPIVSDRGSANQWLLAAGLAYTF"
     gene            complement(723..2651)
                     /gene="cysN"
                     /locus_tag="VFU20_03650"
     CDS             complement(723..2651)
                     /gene="cysN"
                     /locus_tag="VFU20_03650"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HEU5285593.1"
                     /translation="MARPLESDRGLIESDVGAWLAAHEAKEMLRFITCGSVDDGKSTL
                     IGRLLYDSRQIFDDQLASLEADSKRVGTQGDAIDFALLVDGLSAEREQGITIDVAYRF
                     FSSAKRKFIVADTPGHEQYTRNMVTGASTAELAVLVVDARKGVLTQTRRHSYLAHLLG
                     IRRFVLAVTKMDLVDYDQAAFEAICADYRSFADGIGIGEWIGIPLSGLKGDNVLGPSE
                     ATAWYTGPSLVDHLDSVPIDAAEDAAKPLRLPVQWVNRPDQHFRGFAGQIASGAMGPQ
                     TEVRILPSGRQTRIERIVSMDGDLEQAVAGQSVTVTLADEVDCSRGDVIAAAGDPPEA
                     ADQFEATIVWMGEDDLLPGRGYWLKLGTQTVTATVQHPKYEINVNSLEHLAAPTLSLN
                     AIGVAEVATDRDLVFEPYAVPGSSPNRALGGFILIDKLTNATVAAGMIHFALRRALNI
                     HRQHLDVGRDVHARIKGQKPAVLWFTGLSGAGKSTIANLVEKRLVALGKHTFLLDGDN
                     VRHGLNRDLGFTEADRIENIRRVGEVARLMADAGLIVLTAFISPFRAEREMVRKMIPE
                     GEFIEIFVDTPLAEAERRDSKGLYAKARAGELANFTGIDSPYEPPEQPEIHVDTLKLT
                     AEQSAGRIVDELLKRQKV"
     gene            complement(2651..3562)
                     /gene="cysD"
                     /locus_tag="VFU20_03655"
     CDS             complement(2651..3562)
                     /gene="cysD"
                     /locus_tag="VFU20_03655"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HEU5285594.1"
                     /translation="MHAQPPTLTHLDRLEAESIHIMRETVAEAERPVMLYSIGKDSAV
                     MLHLARKAFFPGPLPFPLLHVDTGWKFRDMYALRDKVAAQPGIRLIVHKNPDAEARGI
                     NPFDHGPLHTDLWKTEGLKQALDSHGFDAAFGGARRDEEKSRAKERIFSFRSPNHRWD
                     PKRQRPELWALYNVRKNKGESIRVFPLSNWTELDVWQYIRREAIEVVPLYFAAERPTV
                     ERDGLILLVDDERFRLDGERPQMRSVRFRTLGCYPLSGAIESQAKTVEEIVAEMLLAT
                     TSERQGRAIDRDSGSASMETKKQEGYF"
     gene            complement(3724..5634)
                     /locus_tag="VFU20_03660"
     CDS             complement(3724..5634)
                     /locus_tag="VFU20_03660"
                     /inference="COORDINATES: protein motif:HMM:NF017476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285595.1"
                     /translation="MVEADHDLGQLGHGVGIVGGAGEPLAKDSLRLGDVAGPQRRGGG
                     DDLACGTGSYANGGGIYFGATAVGSYSTADGASAFGSYSNATGDNNTALGFETDATGA
                     SSTAVGAYAQATGAQSTAVGSNAVASGFYSTAIGANAQATGAYFATAVGVNSLASASQ
                     ATALGEFARVTGAYSTAVGMGAYVSGVNSIALGNYVTAAYANAIVIGDYSTTVGADGN
                     IAIGVGADATGFQSVAVGQYAQATGSESTAIGQYSSASGDDSVAVGQYAVASGYYSTA
                     LGEASLASGNYSTALGNSATATAANSVALGYGSLADQVDTVSVGVVGGERRIVNVADG
                     IAATDAATVGQMQAADANLQSQITANDADIAALQASDATQNAAIATLATNDAQQDADI
                     AALQTNDAAQDAAIAQNSADIAALQAASTDNLYIDINSTGAAADATGTDAIAIGEAST
                     ATATNSIALGAGATATHANSVALGSGTATTAANQVNVGGRTISGVAAGAISATSTDAV
                     NGAQLFATNTNVTNLQTGLATTNANVAALGGRVTTLEALAVDFGEDIDDLRDRSDSGS
                     AVAIALSGGTFLPGKSFNLTGNVGAYRGAIAAAVQIGAMVGENAAFNAGIAHGFNEDG
                     QTGVRAGFTFGW"
     gene            5518..>6780
                     /locus_tag="VFU20_03665"
     CDS             5518..>6780
                     /locus_tag="VFU20_03665"
                     /inference="COORDINATES: protein motif:HMM:NF024860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEU5285596.1"
                     /translation="MRAGDVAEAQRVLRERLASAPDDANAMAKLAEIVISLNNIEQAT
                     VLLRRASVASPSPARRLALVQHLQHHVGPAAALQEIEELPAELRSSFDSRAMEAAACG
                     ALGQHDRQIAVYRDMARDFPNNAPVWMSLGNALKTVGLLDEAVDALRRAIGARPTYGE
                     AYWTLANFKSFRFTAADISAMRKALRGKLDDSDALHFHFALGKAFEDRNDYRASFRHY
                     AEGNAIRARSLAPAQMSITPMIANCARLFRPETFRRHSGGGCRDRDPIFVVGLQRSGS
                     TLVEQILASHPQIEGTTELTVIQQIWDRLARRAAAAGRDVFAEIALLPTGAFADIGAE
                     YLERTRPFRLTDRPYFIDKQPANWMHSGLIRLALPNAKIVDARRHPMACGFSNFKQHY
                     ATGVTFSYSLEAIGHFYRDYWRFMNHIDA"
BASE COUNT          995 a         2289 c         2344 g         1152 t
ORIGIN      
        1 aattggacag tcgcgctcgg cgccgcgtgg atccccgact atgtcggctc ggacgattac
       61 cggctgatcc ccgcggccgc ggtccgcggc aagatcggcg ggatcgggat caccacccgc
      121 tcgacctacc tctacgccga cttggtgccg cgcggagcgg gcaaggtcga tttcgacgcc
      181 gggccgatca tcggggcgcg cttcaaccgc accggcaaga tcaaggacga tgtcgtcgac
      241 ctgctgcccg agcgcaagac cgcgatcgag gtcggcgcat tcgtcggcgc cagcctgcac
      301 ggcctcacca acccctatga ttcgctgtcc ttccggctcg acctgctgcg cgacgtcggc
      361 agcgcccacg agtcgaccgt catcgctccg gacatcagtt tctcgacccc gctgtcgcgc
      421 tggacctacg tcagcgcgtc gctgggcgcc gacttcgtcg ccaaccgctt cgccgattat
      481 tatttcagcg tttcgccggc cgaatccgtg ctgaccgggc tgccggcgtt caacgccgac
      541 ggcggcatga agagctggaa gatcggcctg ctcgccaacc agtcggtcac cggcgatctg
      601 accggcgggc tgtcgctgtt cgggacggtc aattattcgc ggctggtcgg cgacttcaag
      661 cgctcgccga tcgtctccga ccgcggcagc gccaaccagt ggctgctcgc cgccgggctc
      721 gcctacacct tctgacgctt cagcaattcg tcgacgatcc ggccggcaga ctgctcggcg
      781 gtcagcttca gcgtgtcgac gtggatctcg ggctgctcgg gcggctcgta ggggctgtcg
      841 atgccggtga aattggcgag ctcgccggcg cgcgccttgg cgtagagccc cttggagtcg
      901 cggcgctcgg cctcggccag cggggtgtcg acgaaaatct cgatgaactc gccttcgggg
      961 atcatcttcc gcaccatctc gcgctcggcc cggaacgggg agatgaaggc ggtcagcacg
     1021 atcagcccgg cgtcggccat cagccgcgcg acttcgccga ctcggcggat gttctcgatg
     1081 cgatcggcct cggtgaagcc caaatcgcgg ttgagcccgt ggcggacatt gtcgccgtcc
     1141 aagaggaagg tgtgcttgcc gagcgcgacg agcctcttct cgaccaggtt ggcgatggtc
     1201 gacttgccgg cgcccgacag gccggtgaac cacagcaccg ccggcttctg gcccttgatc
     1261 cgggcgtgga cgtcgcggcc gacgtcgagg tgctggcggt ggatgttgag cgcccgccgc
     1321 agcgcgaagt ggatcatccc cgcggcgacc gtcgcattgg tcagcttgtc gatgaggatg
     1381 aagccgccga gcgcccggtt cgggctggag cccgggaccg catatggctc gaagaccagg
     1441 tcgcggtcgg tggcgacctc ggcgaccccg atcgcgttca gcgacagggt cggagcggcg
     1501 agatgctcga ggctgttgac gttgatctca tatttggggt gctggaccgt cgcggtcacc
     1561 gtctgcgtgc cgagcttgag ccaatagccg cggccgggca gcaggtcgtc ctcgcccatc
     1621 cagacgatcg tcgcctcgaa ctggtcggcc gcttcgggcg gatccccggc ggccgcgatc
     1681 acgtcgccgc gcgagcagtc gacctcgtcg gccagcgtca cggtcaccga ctggccggcg
     1741 acggcctgct cgagatcgcc gtccatgctg acgatgcgct cgatccgcgt ttgtcggccc
     1801 gacggcagga tccgcacctc ggtttgcggg cccatggctc ccgaggcgat ctggccggca
     1861 aagccgcgga aatgctggtc ggggcggttg acccactgga ctggcaggcg cagcggcttg
     1921 gcggcatcct cggccgcatc gatcggcacg ctgtcgagat ggtcgaccag gctcggcccc
     1981 gtgtaccagg ccgtcgcctc gctcggcccg agcacattgt cgcccttgag gcccgacagc
     2041 ggaattccga tccactcgcc gatgccgatg ccgtccgcga acgagcggta gtcggcgcag
     2101 atcgcctcga acgcggcctg gtcgtaatcg accaggtcca tcttggtgac cgccaggacg
     2161 aagcggcgga tgccgagcaa atgggcgaga tacgaatgcc ggcgcgtctg ggtcagaacg
     2221 cccttgcggg cgtcgacgac cagcaccgcc agctcggcgg tcgaggcgcc ggtcaccatg
     2281 ttgcgggtgt attgctcgtg gccgggagtg tcggcgacga tgaacttgcg cttggcgctc
     2341 gaaaagaagc ggtaggcgac gtcgatggtg atgccctgct cgcgctcggc cgacagcccg
     2401 tcgaccagca aggcgaagtc gatcgcgtcg ccctgcgtgc cgacgcgctt gctgtccgcc
     2461 tcgaggctgg cgagctggtc gtcgaagatc tgccggctgt cgtagagcaa gcggccgatc
     2521 agggtcgact tgccgtcgtc gacgctgccg caggtgatga agcgcagcat ctccttggct
     2581 tcgtgggcgg cgagccaggc gccgacgtcg ctctcgatca ggccgcggtc ggactcgagc
     2641 gggcgggcca tcagaaatag ccctcctgct tcttggtctc catgctggcg cttccggaat
     2701 cgcggtcgat cgctcgcccc tgtcgctcgg aggtggtcgc caggagcatc tcggcgacga
     2761 tctcctcgac ggtcttggcc tggctctcga tggcgcccga cagcggatag cagccgagcg
     2821 tccggaagcg caccgagcgc atctgcggcc gctcgccgtc taggcgaaag cgctcgtcgt
     2881 cgaccagcag gatgaggccg tcgcgctcca ccgtcggccg ctcggcggcg aagtagagcg
     2941 ggacgacctc gatcgcctcg cggcggatat attgccagac gtcgagttcg gtccagttgg
     3001 acagcgggaa gactcggatg ctctcgcctt tgttcttgcg gacattgtag agcgcccaca
     3061 gttcgggccg ctgccgcttc gggtcccagc ggtggttggg gctgcggaag ctgaagatgc
     3121 gctccttggc gcggctcttc tcctcgtcgc ggcgggcgcc gccgaacgcc gcgtcgaagc
     3181 cgtggctgtc gagcgcctgc ttgaggcctt cggtcttcca caggtcggtg tgcagcggcc
     3241 cgtggtcgaa cgggttgatg ccgcgcgctt cggcgtccgg gttcttgtgg acgatcagcc
     3301 ggatccccgg ctgcgccgcg accttgtcgc gaagcgcgta catgtcccgg aacttccagc
     3361 cggtgtcgac atgcagcagc gggaacggca gcgggcccgg gaagaaggcc ttccgggcaa
     3421 ggtgcagcat gaccgccgaa tccttgccga tcgaatagag catgaccggc cgctccgcct
     3481 cggcgacggt ctcgcgcatg atgtggatgc tctcggcctc gagccggtcg agatgcgtca
     3541 gtgtgggcgg ctgtgcgtgc aagtcatgaa actttcgagc gacactctgc ccgcgcgtcg
     3601 gcgacaagtg aagcggtcaa ctgtccgcaa aaggaaacgg acggcgcatc gctgcgccgc
     3661 ccgcctccat tcgctccacg gtcggagccg tcgcttgtcc cgccgaaggc aggcgagcgc
     3721 cggctaccag ccgaaggtga agccggcgcg aacgccggtc tggccatcct cgttgaagcc
     3781 gtgcgcgatg ccggcgttga aggccgcatt ctcgccgacc atcgcgccga tctgcaccgc
     3841 ggcggcaatc gctccgcgat acgcgccgac attgccggtc aggttgaagc tcttcccggg
     3901 caggaaagtg ccgcccgaca gagcgatcgc gaccgccgac ccggagtccg agcgatcccg
     3961 aaggtcgtcg atgtcctcgc cgaagtcgac cgcgagggcc tccagggtcg tcacccggcc
     4021 accgagcgcc gcgacattgg cgttggtcgt cgcgaggccg gtctgcaggt tggtgacgtt
     4081 ggtgttggtc gcgaacaatt gcgcaccgtt caccgcgtcg gtggaggtgg cgctgatcgc
     4141 accggccgcc acgcccgaga tcgtccggcc gccgacgttg acctggttgg ccgcggtcgt
     4201 cgccgttccg gagcccagag cgaccgaatt ggcgtgggtc gccgtcgccc cggcgcccag
     4261 cgcgatgctg ttggtcgccg ttgcagtcga cgcctcgccg atcgcgatcg cgtcggttcc
     4321 ggtcgcatcg gcggctgcgc cggtgctgtt gatgtcgatg tacagattgt cggtgctggc
     4381 tgcctgcagg gccgcgatat cggcggagtt ctgcgcgatg gccgcatcct gcgcagcatc
     4441 gttggtctgc aacgcagcga tgtcggcatc ctgctgggca tcgttggtcg ccagggtcgc
     4501 gatggcggcg ttctgggtcg cgtccgatgc ctgcagggcg gcgatgtcgg catcgttggc
     4561 cgtgatctgc gactgcagat tggcgtcggc tgcctgcatc tggccgaccg tcgcggcgtc
     4621 ggtggcggcg atgccgtcgg cgacgttgac gatccgccgc tcacccccga cgacaccgac
     4681 ggagacggtg tcgacctggt cggcgagcga gccgtagccg agcgcgacgc tgttggcggc
     4741 cgtcgcggtg gcgctgttgc cgagcgcggt cgaatagttc ccgctggcga gcgaggcttc
     4801 gccgagcgcg gtcgaataat agccgctggc gaccgcatat tggccgaccg cgacggaatc
     4861 gtctcccgac gcgctggaat attggccgat cgccgtggat tcgcttccgg tggcttgggc
     4921 atattggccg accgcgaccg actggaagcc ggtggcgtcc gcgcccacgc cgatggcgat
     4981 attgccgtcg gcgccgacgg tcgtcgaata atcgccgatg acgatcgcat tggcataggc
     5041 ggcggtgacg taattgccga gcgcgatgga gttcacgccg ctcacgtagg cgcccattcc
     5101 gacggcggtc gagtaagcgc cggtcacccg ggcgaattcg ccgagcgcgg tggcctggct
     5161 cgcgctggcg agcgagttca cgccgacagc ggttgcgaaa taggctccgg tggcctgggc
     5221 gttggcgccg atcgcggtcg agtagaaacc gctggccacg gcgttcgagc cgaccgcggt
     5281 cgactgggcg ccggtcgcct gggcataggc cccgaccgcc gtcgagctcg cgcccgtcgc
     5341 gtcggtttca aagcccagcg ccgtgttgtt gtcgccggtc gcgttcgaat agctgccgaa
     5401 cgcgctcgcg ccgtccgcgg tcgaataaga gccgacggcc gtcgctccga aatagatacc
     5461 gccgccgttg gcgtaggagc ccgtgccgca ggcgaggtcg tcgccgccac cgcggcgctg
     5521 cgggccggcg acgtcgccga ggcgcagcga gtccttcgcg agcggctcgc cagcgccccc
     5581 gacgatgcca acgccatggc caagctggcc gagatcgtga tcagcctcaa caatatcgag
     5641 caggccacgg tgctgctccg gcgcgcgagc gtcgccagcc cgtcgccggc ccgccggctc
     5701 gcgctggtcc agcatttgca gcatcatgtc ggcccggccg ccgccttgca ggagatcgag
     5761 gagctgccgg ccgagcttcg gtcgagcttc gactcgcgcg cgatggaagc cgcggcgtgc
     5821 ggcgcgctcg ggcaacacga ccggcagatc gccgtctacc gggacatggc ccgggatttc
     5881 ccgaacaatg cgccggtgtg gatgagcctc ggcaatgcgt tgaagaccgt cggcctgctc
     5941 gacgaggcgg tcgacgccct gcgccgggcg atcggcgcgc ggccgaccta cggcgaggcc
     6001 tattggacgc tcgccaactt caaatccttc cggttcaccg ccgccgacat ttcggcgatg
     6061 cgcaaggcgc tgcgcggcaa gctcgacgat tccgacgcgc tgcatttcca tttcgcgctc
     6121 ggcaaggcgt tcgaggaccg caacgactat cgcgcctcgt tccgccatta tgccgagggc
     6181 aatgcgatcc gcgcccggag cctcgctccc gcgcagatga gcatcacccc gatgatcgcc
     6241 aattgcgcgc ggctgttccg gccggagacg ttccggcgcc actcgggcgg cggctgccgg
     6301 gaccgcgacc cgatcttcgt cgtcggcctg cagcgctcgg gctcgaccct ggtcgagcag
     6361 atccttgcca gccacccgca gatcgagggg accacggagc tgacggtcat ccagcagatc
     6421 tgggaccggc tggcgcggcg agcggcggcc gccggccgcg acgtgttcgc ggagatcgcg
     6481 ctcctgccga ccggggcctt tgccgacatc ggcgcggaat atctcgagcg gacgcgcccg
     6541 ttccggctca ccgaccggcc ctatttcatc gacaagcagc cggccaactg gatgcattcg
     6601 gggctgatcc ggctggcgct cccgaacgcg aagatcgtcg atgcccgccg ccacccgatg
     6661 gcgtgcggct tttccaactt caagcagcat tacgcgaccg gggtcacctt ctcctacagc
     6721 ctggaggcga tcggccattt ctaccgcgac tattggcgat tcatgaacca catcgacgcg
//
LOCUS       DASTZO010000130         3884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1108108,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000130 DASTZO010000000
VERSION     DASTZO010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108108"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..510)
                     /gene="paaI"
                     /locus_tag="VFU20_03670"
     CDS             complement(85..510)
                     /gene="paaI"
                     /locus_tag="VFU20_03670"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HEU5285597.1"
                     /translation="MSANDLARKVAHHMLSEEGTGPAWGVTIEEARAGYARLSMPVRG
                     DMLNGHRIVHGGMVFALADTAFAYVCNGRNEKTVAAQASIVFLGSAREGETLVAEAEE
                     LATSGRSGVTRVAVRTADGRPVAEFTGYSRTIGGPVVDV"
     gene            541..1320
                     /gene="paaG"
                     /locus_tag="VFU20_03675"
     CDS             541..1320
                     /gene="paaG"
                     /locus_tag="VFU20_03675"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HEU5285598.1"
                     /translation="MTYETIDFKVDGGIARITLNRPDKLNSFNVAMHMELRDALDSLG
                     NARVLVLTGAGRGFCAGQDLNDRAVASEHPVDLGVTVETCWNPLIRRLTELRQPVIAR
                     VNGVAAGAGANVALACDLVVAARSAKFIQSFTAIGLVPDSGGTWVLPRLVGQARALGL
                     ALTGEPLSADRAAEWGLIWKAVDDDALDAEVDSLAARLAALPPLGLAETKTMIRSTWS
                     RTLDQELNEERNEMRRLGFTDDYREGVSAFLEKRTPNFTGR"
     gene            1335..3359
                     /gene="paaZ"
                     /locus_tag="VFU20_03680"
     CDS             1335..3359
                     /gene="paaZ"
                     /locus_tag="VFU20_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HEU5285599.1"
                     /translation="MKTAQLLNYARDSWIAGEGDLAELPSAIDGSPVALTGSGGLDFS
                     AMLSHARDVGGPALRKLTFHERARMLKSLGLAIMARKEELYELSFSTGATRKDGWIDI
                     EGGAGTLFSFSSKGRRELPDGHVLLDGPLETLSKNGTFMGQHIFTSLQGAAVHINAFN
                     FPVWGMLEKLAPTLLAGVPAIVKPASATAYLTEAAFRIMIESGLLPTGSVQLIVGHVG
                     DLFDHLSCQDVVSFTGSAATAMKLQSHPVIARESVRFVAERDSLNASVLGPDAAPGTP
                     EFDLFVKEVATEMTVKAGQKCTAIRRAMAPAEHLDALEEALRERLGKAKVGDPRSEDT
                     RMGALVSLAQRDDVRGRIAELEAAGARIVAGDPAAEPPVKGGAFLPPVLLRTDDPWST
                     AAVHDVEPFGPVSTIMPYKDLGDAIALANRGRGSLCLSLFTHDGSTARDFVLGAGAFH
                     GRMLVVDRTNAAESTGHGSPLPVLVHGGPGRAGGGEEMGGVRGVKHYMQRTAIQSSPA
                     MIGAIAGAYYVSGAPKRDIDVHPFRKRMSELAVGDTLRTASRTVTLEDIEHFAHFTGD
                     KFYAHMDEEAAKASPIFEGRVAHGYLILSFAAGLFVDPAPGPVLANTGLENLRFLTPL
                     YPGDSMRVELTVRSKSLKTEETGQVRWAVEILNQKDDLVATYDLLTENIP"
     gene            3421..3849
                     /locus_tag="VFU20_03685"
     CDS             3421..3849
                     /locus_tag="VFU20_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5285600.1"
                     /translation="MKINEVMTRDVQTVTPDQTAREAAAFMLRADAGSIPVTDGDRLI
                     GMITDRDIAVRGVAQGHGPDTPVRELMTSGIVCARIDDDIEEVASKMSQAQVRRLPVI
                     DESENLVGIVSLGDLSRETDDETAHQALEGVSAPGGQHQQ"
BASE COUNT          625 a         1424 c         1265 g          570 t
ORIGIN      
        1 gggtcctcga tcgactcgac ggatccgccc ttgtgaagct cggtcgtgta catggcgcct
       61 atctagtccc tgggggccaa aggttcaaac gtcgaccacc ggcccgccga tcgtgcgcga
      121 gtaaccggtg aactcggcga ccgggcggcc gtccgcggtc cgcaccgcga cccgagtcac
      181 tccgcttcgc ccgctcgtcg ccagttcctc ggcctcggcg accagcgtct cgccttcccg
      241 ggcgctaccg aggaacacga tcgaggcctg cgctgcgacg gtcttctcgt tgcggccgtt
      301 gcagacgtaa gcgaaggcgg tgtcggcgag cgcgaacacc atcccgccgt ggacgatccg
      361 gtggccgttg agcatgtcgc cgcgcaccgg catcgacagc cgcgcatagc cggcgcgcgc
      421 ctcctcgatg gtgacgcccc aggcgggccc ggtcccttcc tcgctcagca tgtgatgggc
      481 gaccttgcgg gccagatcgt ttgcgctcac ggcctctcct tcgctaggca cggtgaggcc
      541 atgacttacg agaccatcga cttcaaggtg gacggcggga tcgcccggat caccctcaac
      601 cggcccgaca agctcaacag cttcaacgtc gcgatgcaca tggaattgcg cgacgcgctc
      661 gactcgctgg gcaatgcgcg cgtcctcgtc ctcaccggcg ccggccgcgg cttctgcgcc
      721 ggccaggacc tcaacgaccg ggcggtggcg tccgagcacc ccgtcgacct cggggtcacg
      781 gtcgagacct gctggaaccc gctaatccgc aggctgaccg aattgcgcca gccggtgatc
      841 gcgcgggtca acggggtcgc cgccggcgcc ggcgccaatg tcgcgctcgc ctgcgacctg
      901 gtggtcgccg cccgctcggc gaagttcatc cagagcttca ccgcgatcgg cctggtgccc
      961 gacagcggcg gcacctgggt gctcccgcgc ctcgtcggcc aggcgcgcgc gctcgggctc
     1021 gctctgaccg gcgagccgct gtccgccgac cgggccgccg aatgggggct gatctggaag
     1081 gcggtcgacg acgatgcgct cgacgccgag gtcgactcgc tcgccgcccg gctcgctgcg
     1141 ctgccgccgc tcgggcttgc cgagaccaag acgatgatcc gctcgacctg gtcgcgcacc
     1201 ctcgaccagg agctcaacga ggagcgcaac gaaatgcgcc ggctgggctt caccgacgat
     1261 taccgcgagg gcgtctccgc gttcctcgag aagcgcacgc ccaacttcac cgggcgctag
     1321 ggaaccgacg aacaatgaaa accgcccagc tcctcaatta cgcccgcgac tcatggatcg
     1381 ccggcgaagg cgatctcgcc gagctgccga gcgcgatcga cggctcgccg gtggcgctga
     1441 caggcagcgg cggcctcgat ttctcggcca tgctcagcca cgcccgcgac gtcggcggcc
     1501 cggcactgcg caagctgacc ttccacgagc gggcgcggat gctcaaaagc ctcggcctcg
     1561 cgatcatggc ccgcaaggag gaattgtacg agctcagttt ctcgaccggg gcgacccgca
     1621 aggatggctg gatcgacatc gagggcggcg ccggcaccct gttttccttc tcgtccaaag
     1681 gccgccgcga gcttcccgac ggccatgtgc tgctcgacgg cccgctcgag acgctgtcga
     1741 aaaacggcac cttcatgggc cagcacatct tcacctcgct gcagggcgcg gcggtgcaca
     1801 tcaacgcgtt caatttcccg gtgtggggga tgctcgagaa gctcgccccg actttgctcg
     1861 cgggcgtccc ggcgatcgtc aagccggcct cggcgaccgc ctacctgacc gaggccgcgt
     1921 tccggatcat gatcgaatcc gggctcctgc cgacgggctc ggtgcagctg atcgtcggcc
     1981 atgtcggcga cctgttcgac catttgtcgt gccaggacgt ggtcagcttc accggctccg
     2041 cggcgaccgc gatgaagctc cagagccatc cggtgatcgc gcgcgaatcg gtccgcttcg
     2101 tcgccgagcg cgacagcctc aacgcctcgg tgctcggccc cgacgcggcg cccggaaccc
     2161 ccgagttcga cctgttcgtc aaggaggtcg cgaccgagat gacggtcaag gcgggccaga
     2221 aatgcaccgc tatccgccgc gccatggccc ccgccgagca cctcgacgcg ctcgaggaag
     2281 cgctccgcga gcggctcggc aaggccaagg tcggcgaccc gcgctccgag gacacacgga
     2341 tgggcgctct ggtcagcctg gcccagcgcg acgacgtccg cggccggatc gccgagctcg
     2401 aggcggcggg ggcgcggatc gtcgccggag accccgccgc cgagcccccg gtcaagggcg
     2461 gcgctttcct tcccccggtg ctgctgagga ccgacgaccc ctggtcgacc gcggccgttc
     2521 acgacgtcga gccgttcggc ccggtctcga cgatcatgcc ctacaaggac ctcggcgacg
     2581 ccatcgcgct cgccaaccgc ggccgcggca gcctgtgcct gtcgctgttc acccacgacg
     2641 gctcgaccgc gcgcgacttc gtgctcggcg ccggcgcgtt ccacgggcgc atgctggtcg
     2701 tcgaccgcac caacgccgcc gaatcgaccg gccatggctc gccgctcccc gtcctcgtcc
     2761 atggcggccc cggccgcgcc ggcggcggcg aggagatggg cggggtgcgc ggcgtcaagc
     2821 attacatgca gcgcaccgcg atccagtcgt cgccggcgat gatcggggcg atcgccggcg
     2881 cctattacgt cagcggcgcg cccaagcgcg acatcgacgt ccacccgttc cgcaagagga
     2941 tgagcgagct cgccgtcggc gacacgctca ggaccgccag ccgcaccgtg acgctcgagg
     3001 acatcgagca tttcgcccat ttcaccggcg acaaattcta cgcccacatg gacgaggaag
     3061 cggccaaggc gagcccgatc ttcgaaggcc gcgtcgcgca cggctatctg atcctgagct
     3121 tcgccgccgg actgttcgtc gatccggcgc cgggcccggt gctggccaat acgggcctcg
     3181 agaatctgcg cttcctgacg ccgctctatc cgggcgattc gatgcgcgtc gagctcaccg
     3241 tgcgctccaa gtcgctgaag accgaggaaa ccgggcaggt ccgctgggcg gtcgagatcc
     3301 tcaaccagaa ggacgatctc gtcgccacct acgacctgtt gaccgagaac attccctgac
     3361 cgccggaacg ccgctcctcg gccgttcgtt tccatcgcac accatcccgg gagacgaacg
     3421 atgaaaatca atgaagtgat gactcgcgac gtccagaccg tgactccgga ccagaccgcg
     3481 cgcgaagccg ccgcgttcat gctgcgcgcc gacgccggct cgatcccggt gaccgacggc
     3541 gaccggctga tcggcatgat caccgaccgc gacatcgcgg ttcgcggagt cgcccagggg
     3601 cacggacccg acacgccggt gcgcgagctg atgaccagcg gcatcgtctg cgcccggatc
     3661 gacgacgaca tcgaggaggt cgccagcaag atgagccagg cacaggtccg ccggctgccg
     3721 gtaatcgacg agagcgagaa tctggtcggg atcgtcagcc tcggcgacct cagccgcgag
     3781 accgacgacg agaccgccca ccaggcgctc gaaggcgtca gcgcgccggg cggccagcac
     3841 cagcagtaaa gacagacctt agcggcattc ctccctctcc cgca
//
LOCUS       DASTZO010000131         5214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_818035,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000131 DASTZO010000000
VERSION     DASTZO010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5214
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818035"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1410)
                     /locus_tag="VFU20_03690"
     CDS             complement(<1..1410)
                     /locus_tag="VFU20_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285601.1"
                     /translation="MRVSGWSASVALVALVAPHAVGAQSATPGDQPAAAPAQGTRTTA
                     YDAAFFAQYAPRTALDIARRVPGFTLDLGNIDTRGFAGAAGNVVINGARPSSKAETLE
                     TTLARIPARSVVRVEIGPGDLYGAEYSGKSQVLNVILSAEGGFEGNATLAARRIYTGH
                     VTPTGSASAMLRRGASTVNLSAGADHSFQYEEGSDTLADLVTGDRIEHRRKINSYRNF
                     EPYLSGSWALEHFKDRAVRLNARWSPGRFDLEQRNRVTPAGGPQRDDSLVQDYENPVF
                     ELGGDVTRPLAGGAIKLVGLATRRKRDNFDTYIERDGLLDDGAVTVGGFEQTQKATRN
                     ETIGRLNWSRQDLAGFSFETGGEAVLNTLDSAVDLFVFDEDGNRLRIDLPIDHARVKE
                     KRAEAFVNVGRSLGPALRLDGGLTFEYSRLSVRGDTTADRTLKFWKPKATIDWKPGGG
                     WHAQLSVRRTVAQLDFFDFI"
     gene            1641..3410
                     /locus_tag="VFU20_03695"
     CDS             1641..3410
                     /locus_tag="VFU20_03695"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEU5285602.1"
                     /translation="MSDVFVSYARSSETQARQVAGALNEQGYSVWSDDELPAHRAYGD
                     VIEERLKAARAVVVLWSSEAAKSQWVRAEADAARQAGTLVQATLDGTIPPLPFNQIQC
                     ADLRGWTGGARSPGWDKLAASVAALTGPAAQPGEQHRHWARRDRVSVCVLPFANMSGD
                     PEQEYFSDGISEDITTDLSNVSALEVIARNTAFQFKGQAVDVCEVARKLGVSHVLEGS
                     VRKSGNRVRITAQLIDGQSGGHLWAERYDRDLTDIFAIQDEISKAIVAALKLKLLPEE
                     KKAIEQRGTTSAEAYNLYLMARDYWATGNHGDVGREQRVIRLCERAVEIDPNYARAWA
                     LLAIAQSSLRYGFGIPGEDGAAAAAKALSIDPGVVEAYCPIARRHTEQRKFTEAEIEI
                     AAGLRIDPKSWELHKEGARLHSRQRHFREAAHHLEHEVATEDTDYYAWGMLIGCYHAL
                     GDSAAVRRVAEIALGKAEEALSHDPGSGAALSVAARALAALGESKRAREWIERGLLVD
                     PGNLNMRYNFASVFAAHLGEPEAALALLESTMPKASGALIRWAEIDPDVDSIRSEPRF
                     RSLLDQAKARAATPAETLGPQKA"
     gene            3557..4861
                     /locus_tag="VFU20_03700"
     CDS             3557..4861
                     /locus_tag="VFU20_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285603.1"
                     /translation="MGASVSGMEAAGQQSAEALLGAFQAIGEDERSRHAAVCDYWLSI
                     RGDRDFPTIRDLDPLQISDAGPCSVLLELIGGGEDAEIRHIGENLKAGAEFARIAEAP
                     RRSLLSCIASKLAIVAISRDALAFEDEFETTAGTSRCRVTLLPFSSTGPWVDYVYGFV
                     TSESAPDAAEEAPEIVPEAVEQTPEIVPEAVEQTPETAPEAAEEAPEIVEDALEEAPE
                     VAEDPVEERPEDNMDHAQPTPEMPSDRAQEAPEPAAGESSPAPSESPAESPKGFDPFA
                     GVEGFFGNVVNVELDLPATAAASREPEETGAGPEGTLQGRLAEARAKADEARLARLRS
                     DAALCEGLSAAYDFALDAEDQAEDYLKLVEAQGLKIQLRSPMAPVVRLAFEGRCEEST
                     IAELERVLAWALKMNLPRGSLAERIESEGGIAAILGGPARDQ"
     gene            complement(4913..>5214)
                     /locus_tag="VFU20_03705"
     CDS             complement(4913..>5214)
                     /locus_tag="VFU20_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285604.1"
                     /translation="ETQYCRVVWHCEEFVGLEFETPLAEAVLDRLLNDHKQVTETTIG
                     ELREIAVRAHRLATRQPDADVQSLAELSQRCAVEAVVEGFRLGEGGKAAGPPARG"
BASE COUNT          854 a         1720 c         1881 g          759 t
ORIGIN      
        1 gatgaagtcg aagaagtcga gctgggcgac ggtgcggcga accgaaagct gggcgtgcca
       61 gccgccgccc ggcttccagt cgatggtcgc cttgggcttc cagaatttga gcgttcggtc
      121 ggcggtggtg tcgccccgca cgctgagcct ggaatattcg aaggtcaggc cgccatcgag
      181 ccgcagcgcc gggcccagcg agcggccgac gttgacgaag gcttcggcgc gtttctcctt
      241 cacccgcgca tggtcgatcg gcaggtcgat ccggaggcgg ttcccgtcct cgtcgaagac
      301 gaacaggtcg acggcgctgt cgagcgtgtt gaggaccgcc tcgccgccgg tctcgaacga
      361 gaagccggcg aggtcctggc ggctccagtt gagccggccg atggtctcgt tgcgcgtcgc
      421 cttctgggtc tgctcgaatc cgccgaccgt gaccgcgccg tcgtcgagca gtccgtcgcg
      481 ctcgatatag gtgtcgaaat tgtcgcgctt gcggcgggtc gcgaggccga ccagcttgat
      541 cgccccgccg gccagcgggc gggtgacgtc gccgcccagc tcgaacaccg gattttcata
      601 gtcctggacc aggctgtcgt cgcgctgcgg cccgccggcg ggggtcaccc ggttgcgctg
      661 ctcgagatcg aagcggccgg gcgaccagcg cgcgttgagc cgcacggcgc ggtccttgaa
      721 atgctcgagc gcccagctgc ccgacagata gggctcgaaa ttacggtagc tgttgatctt
      781 gcgccggtgc tcgatgcggt cgccggtgac caggtcggcc agggtgtcgc tgccttcctc
      841 gtactggaag ctgtggtcgg cgccggccga caggttgacg gtcgaggcgc cgcgccggag
      901 catcgccgag gccgagccgg tcggggtcac gtggccggtg tagatccgcc gcgcggccag
      961 cgtggcattg ccttcgaacc cgccctcggc cgagaggatg acgttgagca cctggctctt
     1021 gcccgaatat tcggcgccgt agaggtcgcc agggccgatt tcgacccgga cgacgctgcg
     1081 cgccgggatc cgggccaagg tcgtctcgag ggtttcggcc ttggaactgg gccgggcgcc
     1141 gttgatgacg acattgccgg cggcgccggc gaagccgcgc gtgtcgatgt tgccgaggtc
     1201 gagcgtgaaa ccgggcaccc gccgggcgat gtcgagcgcg gtgcgcggcg catattgggc
     1261 gaagaaggcg gcgtcgtagg cggtggtcct cgtgccctgc gccggggcgg cggcgggctg
     1321 gtcgcccggg gtagcggact gggcgccgac cgcgtggggg gcgaccagcg caaccagggc
     1381 aaccgacgca gaccagcctg aaacgcgcat cgccgactcc tgtattaccg tgctaataca
     1441 gatacacagc ttggcgcgcg aatccagagg gctttcgacc aaccgacgaa cggttccgcc
     1501 agccgccgcg tcggttcggc gagcgggcgg ggaagagccg tgtccgtccc cggcggccgg
     1561 cgccgcagcc ccggaaacgg gttgtcctgc gcggcctgac cctcgtaagc tgctcggcta
     1621 cgaggggaac gacgctgcgg atgtcggacg tatttgtgtc atacgcccgc tcgagcgaga
     1681 cgcaggcgag gcaggtcgcc ggcgcgctca acgagcaagg ctacagcgtc tggagcgacg
     1741 acgagctgcc cgcccaccgc gcctacggcg acgtcatcga ggagcggctg aaagcggcga
     1801 gggcggtggt cgtcctgtgg tcgtccgagg cggcgaagtc gcagtgggtg cgagcggagg
     1861 cggatgccgc gcgccaggcg gggacgttgg tccaggcgac cctcgacggg accatcccgc
     1921 cgctgccgtt caaccagatc cagtgcgccg acctgcgcgg ctggacaggc ggcgcgcgct
     1981 ccccgggctg ggacaagctg gccgccagcg tcgccgcgct cacggggccg gcggcgcagc
     2041 cgggcgagca gcaccggcac tgggcgcgcc gggaccgggt ctccgtctgc gtgcttccgt
     2101 tcgccaacat gagcggcgac cccgagcagg aatatttcag cgacggaatc agcgaggaca
     2161 tcaccaccga cctgtccaac gtgtccgccc tcgaggtgat cgcgcgcaac acggcattcc
     2221 agttcaaggg ccaggcggtc gacgtgtgcg aagtcgcccg caagctcggg gtcagccatg
     2281 ttctggaagg cagcgtccgc aagtccggaa accgggtgcg gatcaccgcc cagctgatcg
     2341 acgggcaaag cggcgggcac ctgtgggccg aacgctacga ccgcgacctg accgacattt
     2401 tcgcgatcca ggacgaaatc tccaaggcga tcgtcgcggc gcttaagctc aagctgttgc
     2461 ccgaagagaa aaaggccatc gagcagcgcg ggacgaccag tgccgaagct tacaacctct
     2521 atttgatggc gcgggattat tgggcgacgg gcaatcatgg cgatgtcggc cgtgagcagc
     2581 gcgtgatccg cctgtgcgag cgcgcggtgg agatcgaccc caattatgcg agggcctggg
     2641 cgctgctggc gatcgcccag tccagcctgc gctacggttt cggaatcccc ggggaagacg
     2701 gcgccgcggc cgccgcgaag gcgctgtcga tcgatccggg cgtggtcgaa gcctattgcc
     2761 ccatcgcgcg ccgccacacc gaacagcgca aatttaccga ggcggaaatc gagatcgccg
     2821 ccggccttcg aatcgacccc aaatcctggg agttgcacaa ggaaggggcc cgactccata
     2881 gccgccagcg gcacttcagg gaggccgcgc accatctgga gcatgaggtc gcgaccgagg
     2941 acaccgacta ttacgcatgg gggatgctga tcggctgtta tcatgcgctc ggcgattccg
     3001 cggcggtgcg gcgggtcgcg gagatcgccc tgggcaaggc cgaagaggca ctcagccatg
     3061 accccggcag cggcgcggcg ctcagcgtcg ccgcgcgtgc ccttgccgcg ctcggcgaga
     3121 gcaagcgtgc acgggagtgg atcgagcggg gccttctggt cgatccgggc aatttgaaca
     3181 tgcgctacaa tttcgcgtcc gtgtttgccg cgcacctcgg cgagcccgaa gcggcgctgg
     3241 cgctgctcga gtcgaccatg ccgaaagcca gcggggcact gatccgctgg gccgaaatcg
     3301 accccgacgt cgacagcatc cgctcggagc cgcgattccg gtctttgctc gaccaggcga
     3361 aagcacgagc ggcgacccca gcggaaaccc ttggcccgca aaaggcttga gcctgccttc
     3421 gccttgaaca ggcggcatcg cacagctgaa tccacgcccg gcccaatcga gcgccggcgc
     3481 tcccgatcca aagcttaacc attttccaag aatgagcggc gcacaaccgg ttcgaacggg
     3541 agcggaaggg gagacgatgg gggcgtcggt ttcagggatg gaggcggccg ggcagcagtc
     3601 ggcggaggcg ctgctgggcg cgttccaggc gatcggcgag gacgaacgga gccggcacgc
     3661 ggcggtctgc gattactggc tgtcgatccg cggcgaccgc gactttccga ccattcgcga
     3721 cctcgacccg ctgcagattt ccgatgccgg gccgtgcagc gttctgctcg agctgatcgg
     3781 cggcggcgag gacgccgaga tccgccatat cggcgagaat ctgaaggccg gcgccgagtt
     3841 cgccaggatt gccgaggccc cgcgccgttc gctgctgtcg tgcatcgcca gcaagctcgc
     3901 catcgtcgcc atctccaggg acgcgctggc gttcgaagac gaattcgaga ccacggccgg
     3961 aacgtcgcgc tgccgggtga ccctgctgcc gttcagctcg accgggccgt gggtcgacta
     4021 cgtctacggg ttcgtgactt cggaaagcgc cccggacgcg gccgaagaag cgccggagat
     4081 cgtcccggag gcggtcgaac aaacgcccga gatcgtcccg gaggcggtcg aacaaacgcc
     4141 cgagaccgcc ccggaggcgg ccgaagaggc gccggagatc gtcgaagacg cgctcgaaga
     4201 ggcgccggag gttgccgaag atccggtcga agaaaggccc gaggacaata tggaccatgc
     4261 ccaacccact cccgagatgc cgtcggaccg agcgcaagag gcgccggagc ccgccgcggg
     4321 cgaatcgtcg ccggctcctt ccgaaagccc ggccgagtcg ccgaaaggct tcgatccgtt
     4381 cgccggagtc gagggctttt tcggcaatgt cgtcaacgtc gagctggacc ttccggccac
     4441 ggccgccgcc agccgggagc ccgaggaaac cggcgccggg ccggagggca cgttgcaggg
     4501 ccggctggcc gaagcccggg cgaaagccga cgaggcccgg ctcgcccggc tgcggagcga
     4561 tgccgcgctc tgcgagggcc tgagcgcggc ctatgacttc gcgctcgacg ccgaggacca
     4621 ggcggaggac tatctcaagc tcgtcgaggc gcaggggctg aaaatccagc tgcgctcgcc
     4681 gatggcgccg gtggtccggc tggcgttcga gggccggtgc gaggaatcga cgatcgccga
     4741 gctcgagcgg gtcctggcct gggcgctgaa gatgaacctg ccgcgcggct cgctcgccga
     4801 gcgcatcgag tccgaaggcg ggatcgcggc gatcctcggc ggaccggcgc gggatcaata
     4861 agctttcgag tcgagtgacg ggcgggcggc ggccgtcacc ggccagcgcc gcttacccgc
     4921 gcgcgggcgg cccggcggcc ttgccgccct cgccgagccg gaagccctcg accaccgcct
     4981 cgacggcgca gcgctgcgac agctccgcca ggctctggac gtcggcgtcg ggctgcctgg
     5041 ttgccagccg gtgagcccgg acggcgatct cgcgcagctc gccgatcgtc gtctcggtca
     5101 cctgcttgtg gtcgttgagc agccggtcga ggacggcctc ggccagcgga gtctcgaact
     5161 cgagcccgac gaactcctcg cagtgccaga cgacgcggca atattgggtc tcga
//
LOCUS       DASTZO010000132         7241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_872997,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000132 DASTZO010000000
VERSION     DASTZO010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7241
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872997"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1067
                     /locus_tag="VFU20_03710"
     CDS             153..1067
                     /locus_tag="VFU20_03710"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HEU5285605.1"
                     /translation="MTSSALDSAATRLPADWRDLLALTKPRVMSLVVFTGLAGMLAAP
                     VLPPLAIGFTAILCIALGAGAAGALNQWYEADLDAKMKRTASRPLPAGRMDRQTALHF
                     GVGLGVFSVVLMDVAANHLAALLLAASILFYVLVYTVWLKRRTAQNIVIGGAAGAFPP
                     LIGWVAATGELAILPAILFAIIFLWTPPHFWALSLFVRSDYAAAKVPMLPVTAGIAAT
                     RRQIVLYSLPMAAAAVAPWPLGLAGPLYGLVAAGLGAAFLIMAGQVLANRSADPARMR
                     PEKRLFGFSIIFLFALFAALVADRWISA"
     gene            1064..1192
                     /locus_tag="VFU20_03715"
     CDS             1064..1192
                     /locus_tag="VFU20_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285606.1"
                     /translation="MTRHEDEVVRRQRERARLMAWLLGGFVVLLFLVTVAKIGLNR"
     gene            1189..1722
                     /locus_tag="VFU20_03720"
     CDS             1189..1722
                     /locus_tag="VFU20_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEU5285607.1"
                     /translation="MNRETRTALLAAFLAATMVGVSFAAVPLYQLFCEVTGFGGTPVR
                     AEQAPGAVAGEVGVRFDANIDPKLPWRFEPIERTIRIAPGARTQVSYRATNLTARATT
                     GQAMFNVTPAKAGQYFSKIECFCFVEQTLKGGQSVDMPVIFFVDPKIRTDPATRDIDE
                     ITLSYTFYPVETPGRGG"
     gene            1757..2575
                     /locus_tag="VFU20_03725"
     CDS             1757..2575
                     /locus_tag="VFU20_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEU5285608.1"
                     /translation="MAATKHHDYHLVDPDPWPIIGAIGAFALFGGAVMWMHDNPYGKF
                     VSLGGLLVVLVTMANWWANTIREAHTGYHTPVVQLHLRYGMILFIASEVMFFLAWFWA
                     FFDASLFPSAVEAVGAQWPPKGIEVLDPWGFPLLNTMILLCSGTTVTWAHHALIHGDR
                     HGLKLGLLLTILLGLLFTSIQAYEYIHAPFDFKGNIYGATFFMATGFHGFHVIVGTIF
                     LTVCLLRARQGHFTPRQHFGFEAAAWYWHFVDVVWLFLFVSIYVWGGWGAPVHG"
     gene            2592..2948
                     /locus_tag="VFU20_03730"
     CDS             2592..2948
                     /locus_tag="VFU20_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEU5285609.1"
                     /translation="MSGPSPLAAAIAGACPRCHARSLFDGWVSFAPQCSNCGLDFSAF
                     NVGDGPAAFLILVVGAILTAAAIAVELLFEAPFWVHLVWIPLGLALTIFGLRLGKALL
                     LAQEYRHQAGEGRIRP"
     gene            2945..3544
                     /locus_tag="VFU20_03735"
     CDS             2945..3544
                     /locus_tag="VFU20_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEU5285610.1"
                     /translation="MIRKWPLVPTLVVGLAVAIMIGLGIWQLQRAGEKDQLLARYRSA
                     QNLPPIAFPTAPVHSDNLPLFRHATGVCLRPVSKRSTAGQNRSGEPGFVHIVDCSTGA
                     EGPGMSVEVGWSKNPNFAVNWPGGPVSGIIAPDSKSRMRLVAATAPPGLEPSAPPSIA
                     QIPNNHRSYAVQWFLFALIAAVIYVLALRRKQKDEAKPR"
     gene            3541..4770
                     /locus_tag="VFU20_03740"
     CDS             3541..4770
                     /locus_tag="VFU20_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5285611.1"
                     /translation="MTAELHRLANGLSVAIDPLPGAESVALGLYAKVGSRSEPEPLSG
                     LAHLVEHMVFKGAGGRDTRQLAEAIEDVGGHLNAWTSRDQTAFHGRALARDLPLVAEL
                     VADLVRAPHFDDEHLAREKQVILSELGECIDSPDDLVHDHLFEAAFGGQAIGRSVLGR
                     EDSVRAIKRDDCVAWLEQQFVPSRLILAASGKVDGDTLLALAERLLGDMDARPEPAIE
                     PARFTGGVRNDRRVFEQAHWCLALPCLCSADPGVPALSIFVQALGGGTSSRLFQELRE
                     ERGLAYSIYAWNQGFADSGLVGIGCAAERARAAESIALARELLGQAAETLSEAEVQRA
                     RAQIEAGLLMGQETAQGRADHIARSIEIFGRIVLIDELVADLRAVDGAAARAAGAALL
                     DGPVAVASVGAKLALAA"
     gene            4767..5660
                     /locus_tag="VFU20_03745"
     CDS             4767..5660
                     /locus_tag="VFU20_03745"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5285612.1"
                     /translation="MSPRSTPLLETIVAEPWEDWGLVDSGHGRKLERYGPVTVVRPEP
                     QAMWAPAADAWDPDATFVPASDEEGGGRWVEHRPVPREWPLSRGAARFHASLTPFRHL
                     GFFPDMAPQWDWMRARIEGKGSGDKDVLNLFGYTGVGTLLLSDAGARLVHVDASKKSV
                     EGGKANAALSGMTERPVRWIVDDARKFTAREVRRGRRYDGILLDPPKFGRGPEGEVWR
                     LEEHLGGLLADCRTLLDADSRFLVLTVYAVRMSALAIGELVRQALGPLGGSVEAGEMA
                     VREEARGLLLPTAIFARWSRD"
     gene            complement(5657..6295)
                     /locus_tag="VFU20_03750"
     CDS             complement(5657..6295)
                     /locus_tag="VFU20_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEU5285613.1"
                     /translation="MIGPVIARDVRRALGGAAWLPVAFFLLVATLVPFAVGPDARLLE
                     RIGGGALWIAALTAALLPVERLVEPDRADGMLDQLALHGLTEEAVAVARIIAHWLTFA
                     PLLLLAAVPGSVLLGLGAEGLRSSLAALAVGTPGLAALAVAVAALTAGLPRAGALAGI
                     LLLPLAVPLLIFGAAASGDGGSGALLLEAAVSLALCAGAPFAAGAAIRAGRT"
     gene            complement(6292..6873)
                     /gene="ccmA"
                     /locus_tag="VFU20_03755"
     CDS             complement(6292..6873)
                     /gene="ccmA"
                     /locus_tag="VFU20_03755"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HEU5285614.1"
                     /translation="MKPLLRFEAVTLRRGGRLLFEGLDLKLGPGEALHVTGPNGSGKT
                     SLIRLAAGLLRAEAGRVERTAPALSDEALALDRELPLAKALRFWARLGGTAGKLDAAM
                     AAMGLAHLADVPVRLLSSGQAKRATLARVAASGAKLWLLDEPLNGLDADGIGRLEALV
                     ERHRASGGAVVAASHGPLGGDWERLELGQGATA"
     gene            complement(6870..7232)
                     /locus_tag="VFU20_03760"
     CDS             complement(6870..7232)
                     /locus_tag="VFU20_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010236253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HEU5285615.1"
                     /translation="MEAIARATLDALPEPFASHLKDVVLLVEEFASDEILESMGIDDP
                     FDLTGLYEGLPLNQKSVDISGTLPDRILLFRRPILDEWADGEHSLERLVAHILIHEAG
                     HHFGFSDADMHALEESVR"
BASE COUNT          996 a         2622 c         2497 g         1126 t
ORIGIN      
        1 gtcaacctgt tctggtcgct ggccgccggc aagaaggctc cggacaaccc gtggggcgag
       61 ggcgcgacga cgctcgaatg gactcttccg agcccgccgc cgttccacca gttcaacacg
      121 ctgcccaaga tcgactgagg cccgctcgac cgatgaccag cagcgccctc gacagcgccg
      181 cgacccgcct ccccgccgac tggcgcgatc tgctggcgct gaccaagccc cgggtgatga
      241 gcctggtcgt gttcaccggg ctcgccggaa tgctcgcggc gcccgtgctg ccgccgctcg
      301 cgatcggctt caccgcgatc ctgtgcatcg cattgggcgc cggcgccgcc ggggcgctca
      361 accaatggta tgaggccgac ctcgacgcca agatgaagcg gaccgccagc cgaccgctcc
      421 cggccgggcg gatggaccgc cagaccgcgc ttcacttcgg ggtcgggctc ggggtgttct
      481 cggtcgtgct gatggacgtc gccgccaacc acctcgccgc gctgctgctc gccgcctcga
      541 tcctgttcta cgtgctcgtc tacaccgtct ggctgaagcg gcggaccgcc cagaacatcg
      601 tcatcggcgg cgccgccggg gccttcccgc cgctgatcgg ctgggttgcc gcgaccggcg
      661 agctcgcgat cctgccggcg atcctgttcg cgatcatctt cctgtggact ccgccgcact
      721 tctgggcgct gtcgctgttc gtccgctccg attatgccgc cgccaaggtg ccgatgctac
      781 cggtgaccgc gggcatcgcc gcgacccggc ggcagattgt cctgtacagc ctgccgatgg
      841 cggccgccgc ggtggcgccg tggccgctcg gccttgcggg gccgctgtac gggctggtcg
      901 cggcgggcct gggcgccgcg ttcctgatca tggccgggca ggtgctcgcc aaccgctcgg
      961 ccgatccggc gcggatgcgg cccgagaagc ggctgttcgg cttttcgatc atcttcctgt
     1021 tcgctttgtt cgccgccctg gtcgccgacc gctggatttc cgcatgaccc ggcacgagga
     1081 cgaggtcgtc cggcgccagc gcgagcgcgc ccggctgatg gcgtggctgc tgggcggctt
     1141 cgtcgtgctg ctgttcctgg tcacagtcgc caagatcggg ctcaaccgat gaaccgggag
     1201 acccgcaccg cgctgctggc ggcgttcctc gccgcgacca tggtcggcgt gtcctttgcc
     1261 gcggtgccgc tctaccagct gttctgcgaa gtcaccggct tcggcggcac tccggtccgc
     1321 gccgagcaag ctccgggggc ggtcgcgggc gaggtcggcg tgcgcttcga cgccaacatc
     1381 gaccccaagc tgccgtggcg gttcgagccc attgagcgca ccatccggat cgcgcccggc
     1441 gcccgcaccc aggtctcgta ccgggcaacc aacctcaccg cgcgggcgac gaccggccag
     1501 gcgatgttca acgtcacccc ggccaaggcc ggacaatatt tctcgaagat cgaatgcttc
     1561 tgcttcgtcg agcagacctt gaagggtggg cagagcgtcg acatgccggt gatcttcttc
     1621 gtcgacccga aaatccgcac ggatccggcg acccgggaca tcgacgagat caccctgagc
     1681 tacacatttt acccggtgga aactccgggc cggggcggct agagccgctc gcaccagaga
     1741 gggaagaacg acccagatgg cggcgaccaa gcaccacgat taccacctcg tcgaccccga
     1801 tccgtggccg atcatcggcg ccatcggcgc gttcgccctg ttcggcggcg cggtgatgtg
     1861 gatgcacgac aacccctacg gcaagttcgt ctcgctgggc gggctgctgg tggtgctggt
     1921 gacgatggcc aattggtggg ccaacaccat ccgcgaggcg cacaccggct accacacgcc
     1981 ggtcgtccag ctgcacctcc ggtacgggat gatcctgttc attgcctcgg aggtgatgtt
     2041 cttcctcgcc tggttctggg cgttcttcga cgcctcgctg ttcccgtccg cggtcgaggc
     2101 ggtcggcgcg caatggccgc ccaagggcat cgaggtgctc gacccgtggg gcttcccgct
     2161 tttgaatacg atgatcctgt tgtgctcggg aaccaccgtc acctgggccc accacgcgct
     2221 gatccacggc gaccgccacg gcctcaagct cggccttttg ctgacgatcc tgctcggcct
     2281 gctgttcacc agcatccagg cctatgagta catccacgcg ccgttcgact tcaaaggcaa
     2341 tatctacggc gcgaccttct tcatggcgac cggcttccac ggcttccacg tgatcgtcgg
     2401 gacgatcttc ctgaccgtct gcctgctccg cgcgcgccag ggccatttca cgcccaggca
     2461 gcatttcggc ttcgaggccg cggcctggta ctggcacttc gtcgacgtcg tctggctgtt
     2521 cctgttcgtg tcgatctacg tgtggggcgg ctggggcgcg ccggtccacg gctgatgcat
     2581 cggcgctcgc cgtgagcggg ccttcgccgc tggccgcagc gatcgccgga gcgtgcccgc
     2641 gctgccatgc gcgcagcctg tttgacggct gggtgagctt cgccccgcaa tgctccaatt
     2701 gcggcctcga cttttccgct ttcaacgtcg gcgacggacc ggcggcgttc ctgatcctgg
     2761 tcgtcggggc gatcctgacg gcggccgcga tcgcggtcga gctgctgttc gaggcgccgt
     2821 tctgggtgca tctcgtctgg atcccgctgg ggctcgcgct gaccatcttc gggctgcgcc
     2881 tcggcaaggc gctgctgctc gcgcaagaat atcgccacca ggcgggcgag gggcgcatcc
     2941 ggccatgatc cgcaagtggc cgctggtccc gaccctggtc gtcggcctcg ccgtcgcgat
     3001 catgatcggc ctcggcatct ggcagctgca gcgcgccggc gagaaggatc agctgctcgc
     3061 ccgctaccga tccgcgcaga atttgccgcc gatcgccttc ccgaccgcgc cggtccacag
     3121 cgacaatctg ccgctgttcc gccacgcgac cggggtgtgc ctgcggccgg tcagcaagcg
     3181 cagcaccgcc ggccagaacc gctccggcga gcccggtttc gtgcatattg tcgattgcag
     3241 caccggcgcc gaggggccgg ggatgagcgt cgaggtcggc tggtcgaaga accccaattt
     3301 cgcggtcaac tggccgggcg gtccggtcag cgggatcatc gcgcccgaca gcaagtcgcg
     3361 gatgcggctg gtcgcggcga ccgctccgcc ggggctcgag ccgagcgccc cgccgtcgat
     3421 cgcccagatc cccaacaacc accgctccta cgcggtgcaa tggttcctgt tcgcgttgat
     3481 cgcggcggtg atctacgtcc tcgcgctgcg caggaagcag aaggacgagg cgaagccgcg
     3541 atgaccgccg agctgcaccg cctggcgaac gggctgtcgg tcgcgatcga cccgcttccc
     3601 ggagcggaat cggtggcgct cggcctctat gccaaggtcg gctcgcgctc cgaacccgag
     3661 ccgctgtcgg gcctcgccca cctggtcgag catatggtgt tcaagggcgc cggggggcgg
     3721 gacacgcggc agctcgccga ggcgatcgag gacgtcggcg gccacttgaa cgcctggacg
     3781 tcgcgcgacc agaccgcgtt ccacggccgc gctctcgcca gggacctgcc tttggtcgcc
     3841 gagctggtcg ccgacctggt ccgggcgccg catttcgacg acgagcatct ggcccgcgaa
     3901 aagcaggtga tcctgtccga gctcggcgag tgcatcgatt cgcccgacga tctcgtccac
     3961 gaccatctgt tcgaggcggc gttcggcggc caggcgatcg gccgctcggt gctcggccgc
     4021 gaggacagcg ttcgcgccat caagcgcgac gattgcgtcg cctggctcga acagcaattc
     4081 gtcccgtcgc ggctgatcct cgcggcgagc ggcaaggtcg acggcgacac gctgctcgcc
     4141 cttgccgagc ggctgctcgg cgacatggac gcgaggcccg agcccgcgat cgagccggcg
     4201 cgcttcaccg gcggagtccg caacgaccgg cgggtcttcg agcaggcgca ctggtgcctg
     4261 gcgttgccct gcctttgcag cgccgatccc ggagtgccgg cgctgtcgat cttcgtccag
     4321 gcgctgggcg gcggcacctc gtcgcggctg ttccaggagc tgcgcgagga gcgcggcctc
     4381 gcttattcga tctacgcgtg gaaccagggc ttcgccgaca gcggcctggt cggcatcggc
     4441 tgcgcggccg agcgcgcgcg ggccgcggaa tcgatcgccc tcgcccgcga gctgctcggc
     4501 caggccgccg agaccctgag cgaggcggaa gtgcagcggg cgcgggcgca gatcgaggcc
     4561 ggcctgctga tgggccagga aaccgcccag ggccgcgccg accacatcgc ccgctcgatc
     4621 gagattttcg gccggatcgt tctcatcgac gagctggtcg ccgacctccg ggcggtcgat
     4681 ggcgcggcgg cgcgggcggc cggcgccgcc ttgctcgacg ggccggtcgc ggtcgcctcg
     4741 gtcggggcga agctcgcgct ggccgcgtga gcccgcgttc cacccccttg ctcgaaacga
     4801 tcgtcgccga gccgtgggaa gattgggggc tggtcgacag cggccatggc cgcaagctcg
     4861 agcgctacgg gccggtgacg gtggtccggc ccgagccgca ggcgatgtgg gcgccggcag
     4921 ctgacgcctg ggaccccgac gcgaccttcg tcccggcttc cgacgaggag ggcggcgggc
     4981 gctgggtcga gcaccgtccg gtcccgcgcg aatggccgtt gtcgcgcggc gcggcccgct
     5041 tccatgcgtc gctgaccccg ttccgccacc tcggcttctt ccccgacatg gcgccgcaat
     5101 gggactggat gcgcgccagg atcgagggaa aaggttccgg ggacaaggac gtgctcaacc
     5161 tgttcggcta caccggcgtc ggcacgctgc tcctgagcga cgccggagcg cggctggtcc
     5221 atgtcgacgc ctcgaagaaa tcggtcgagg gtggcaaggc caacgccgct ttgtcgggaa
     5281 tgacggagcg gccggtccgc tggatcgtcg acgacgcgcg caagttcacc gcccgcgagg
     5341 tccggcgcgg ccgccgctac gacggaatcc tgctcgaccc gcccaagttc ggccgcggcc
     5401 cggaaggcga ggtttggcgg ctcgaggagc atcttggcgg tctgctcgcc gactgccgca
     5461 ccttgctcga cgcggacagc cgcttcctcg tcctcaccgt ctatgcggtg cgaatgtcgg
     5521 cgctggcgat cggcgagttg gtccgccagg cgcttggccc tctcggcggg agcgtcgagg
     5581 ccggcgagat ggcggtgcgc gaggaggcgc gcgggctgct tctgccgacc gccatcttcg
     5641 cgaggtggag cagggactag gtccgcccgg cgcggatcgc ggcgccggcg gcgaacggcg
     5701 ccccggcgca cagcgccagc gacaccgcgg cttccaagag caaggcgccc gagccgccgt
     5761 ccccgctcgc cgcggccccg aagatcagca gcggcaccgc cagaggcagc agcaggatgc
     5821 ccgcgagcgc cccggcgcgc ggcagtcccg ccgtcagcgc ggcgaccgcg accgccagcg
     5881 ccgccagccc cggggtcccg accgccagcg cggccagaga gcttcgcagc ccttccgcgc
     5941 ccagcccgag cagcaccgag cccggcaccg cggcgagcag cagcagcggc gcgaaggtca
     6001 gccagtgggc gatgattctg gcgaccgcga ccgcttcctc ggtgaggccg tggagcgcga
     6061 gctggtcgag cattccgtcg gcgcggtcgg gctcgaccag ccgctcgacc ggcagcagcg
     6121 ccgcggtcag cgccgcgatc cacagcgcgc cgccgccgat ccgctcgagc agccgggcgt
     6181 cgggcccgac cgcgaatggc accagcgtcg cgaccagcag gaagaaggcg accggcagcc
     6241 acgccgcccc gccgagcgct cgacgaacgt cgcgcgcaat caccggcccg atcacgccgt
     6301 cgccccctgg ccgagctcca gccgctccca gtctccgccg agcggcccgt gcgaggcggc
     6361 gaccaccgct ccgcccgacg cgcgatggcg ttcgaccagc gcctcgagcc gcccgatgcc
     6421 gtcggcgtcg aggccgttca gcggctcgtc gagcagccac agcttggccc cggacgcggc
     6481 gacccgggcc agcgtcgccc gcttcgcctg gcccgacgac agcagccgca ccggcacgtc
     6541 ggcgagatgg gcgagcccca tcgccgccat cgctgcgtcg agcttgccgg ccgttccgcc
     6601 gagccgggcc cagaaccgca gcgccttggc cagcggcagc tcgcggtcga gcgccagcgc
     6661 ctcgtccgac agcgccggag cggttctctc gactcggccg gcctcggccc gcaacagccc
     6721 tgccgcgagc cggatcaggc tggtcttgcc gctgccgttg gggcccgtca cgtgcagcgc
     6781 ctcgccgggc ccgagcttga ggtcgaggcc ttcgaacagc agccgaccgc cacgccgcag
     6841 agtcaccgct tcgaaccgca gcagcggctt caccgaaccg attcctcgag cgcgtgcatg
     6901 tcggcgtcgg aaaagccgaa atggtggccg gcctcgtgga tcaggatgtg cgcgacgagc
     6961 cgttcgaggc tgtgctcgcc gtccgcccat tcgtcgagga tcgggcgcct aaacagcagg
     7021 atccggtcgg gcagggtgcc ggagatgtcg acgctcttct ggttgagcgg aagcccttcg
     7081 tagaggcccg tcaggtcgaa cggatcgtcg atccccatcg attccaatat ctcgtcgctg
     7141 gcgaactcct cgaccagcag gacgacgtcc ttcagatggc tggcgaaggg ctcgggcaga
     7201 gcgtcgagcg ttgccctggc gatcgcctcg atgtccgcgg c
//
LOCUS       DASTZO010000133         2844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1081574,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000133 DASTZO010000000
VERSION     DASTZO010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081574"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..1197)
                     /locus_tag="VFU20_03765"
     CDS             complement(19..1197)
                     /locus_tag="VFU20_03765"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HEU5285616.1"
                     /translation="MTRSLLLAAAAAFALASAAPAKAPQYETAAPIAYLIDLSSGAEL
                     LAKDADRRIPPASMAKMMTTEVAFELIDRGQLPLAKMCTVRPETWRQWHGPQAGSTMF
                     LSANEQVSVENLLHGIVTLSGNDASVVLAECIAGTEQAFAGQMNALARKLGLTNSHFG
                     NSNGWPDEGRTYVTARDLAALARATIEKHPKLYKQFYGQPSFTWGKTLGSGQAITQGN
                     RNPILGKVPGADGLKTGHTAEAGYGFTGSAEQNGRRLVMVVAGLSSWNQRVEESVRLM
                     SWGFNAWTARPLYRAGAKVGTARVQLGDDGEVDLVAPRNLAVTVPAGLASGPTKLRIR
                     YQGPVKAPIAKGQHVADLVVVTGDTPPQVVPLVAGEEIEEAGFFGRIWIGFKQLVGMA
                     "
     gene            complement(1250..2356)
                     /locus_tag="VFU20_03770"
     CDS             complement(1250..2356)
                     /locus_tag="VFU20_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HEU5285617.1"
                     /translation="MLSGWTLVGLAAVAFGPASAQVDPLAPRPAPPQAQPAPPLVQVA
                     PPQTYAAPTGFAAYKIYLMAKARHGGVREATIQATVPYLELNSRAIELDRAQQPRPRT
                     SNYMAPFAPYRREHVTEVLIARGQSRYYQHWPNLQLIQARHGVDPAVLMAIYGHETSY
                     GSITGRFDLLEVLATLAYEGRRRAMFETEFIATLKLIDNGVSRARLRGSYAGATGYPQ
                     FMPTAALRLRVDGDGDGHANIWSNEVDALASIANYLREAGWKPGVPWGIAVQVPSTLN
                     RTAIRSMLNAPRCPLVHKRHSRWLTMREWRSLGVAPTGRTLPENEMATLLEPDGPGAR
                     AYLLTTNYRAILDYNCSNYYALAVGLLADAIARR"
     gene            2480..2569
                     /locus_tag="VFU20_03775"
     tRNA            2480..2569
                     /locus_tag="VFU20_03775"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2514..2516,aa:Ser,seq:tga)
     gene            2653..>2844
                     /locus_tag="VFU20_03780"
     CDS             2653..>2844
                     /locus_tag="VFU20_03780"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5285618.1"
                     /translation="MSDSANPQERPERRRANRIALRLGATMREPNRGRVEARLIDIST
                     HGCRVEAISGASPDSWLWLN"
BASE COUNT          426 a          965 c          996 g          457 t
ORIGIN      
        1 ggctgatgaa gcggcccttc acgccatccc gacgagctgc ttgaagccga tccagatgcg
       61 gccgaagaag ccggcttcct cgatctcctc gccggcgacc aagggcacca cctgcggggg
      121 tgtgtcgccg gtgacgacga cgaggtcggc gacgtgctgg cccttggcga tcggcgcctt
      181 gaccggcccc tggtagcgga tcctgagctt ggtcgggccg ctcgccaggc cggcgggcac
      241 ggtgacggcc aaattgcgcg gagcgaccag gtcgacctcg ccgtcgtcgc cgagctggac
      301 ccgggcggtg ccgaccttcg cgcccgcccg gtacaagggc cgcgcggtcc aggcgttgaa
      361 gccccagctc atcagccgca ccgattcctc gacgcgctgg ttccagctcg acaggccggc
      421 gaccaccatc accagccgcc ggccgttctg ctcggccgag ccggtgaagc cgtagccggc
      481 ctcggcggtg tggccggtct tcagcccgtc ggcgccgggc accttgccga gaatcgggtt
      541 gcggttgccc tgggtgatcg cctgccccga gcccagggtc ttgccccagg tgaagctcgg
      601 ctggccgtag aactgcttgt agagcttcgg atgcttctcg atcgtcgccc gggccagggc
      661 cgcgaggtcg cgcgcggtca cgtaggtgcg cccctcgtcg ggccaaccgt tgctgttgcc
      721 gaaatggctg ttcgtcaggc ccagcttccg cgccagagcg ttcatctgtc cggcgaatgc
      781 ctgctcggtc ccggcgatgc actcggccag caccaccgac gcgtcgttgc ccgacaaagt
      841 gacgatcccg tgcagcagat tctcgacgct gacctgctcg ttcgccgaca ggaacatggt
      901 cgatcccgcc tgcggcccgt gccactggcg ccaggtctcg ggccggaccg tgcacatctt
      961 cgcaagcggc agctggccgc ggtcgatcag ctcgaaggcg acctcggtgg tcatcatctt
     1021 ggccatcgac gccggcggga tgcgccggtc ggcgtccttg gcgagcaatt cggcgcccga
     1081 cgacaggtcg atcaggtagg cgatcggtgc ggcggtttcg tattgcggcg ccttggcggg
     1141 ggctgcggaa gcgagtgcga aagccgcggc ggcggcgagc aaaagtgagc gagtcatacg
     1201 tcccgtctat cgcccgggct cgccttgcgc gagagccttc ggagcgcttt cagcgccggg
     1261 cgatggcgtc cgccagcagc ccgaccgcca gagcgtaata attcgagcaa ttataatcga
     1321 ggatcgcccg gtagttggtg gtcagcagat aggcccgggc gccgggcccg tcgggctcga
     1381 gcagggtcgc catctcattc tcgggaagag tccggccggt gggcgcgacc ccgagcgagc
     1441 gccattcgcg catcgtcagc cagcggctgt ggcgcttgtg caccagcggg cagcgcggag
     1501 cgttgagcat gcttcggatc gcggttcggt tgagggtcga gggcacttgc accgcgatcc
     1561 cccacgggac cccgggcttc caccccgcct cgcgcaggta attggcgatc gaggccaggg
     1621 catcgacctc gttcgaccag atgttcgcat ggccgtcgcc gtcgccgtcg accctgagac
     1681 gcagcgccgc ggtcggcatg aactgcggat atccggtcgc gccggcatag ctgcctcgta
     1741 gccgcgcccg cgacacgccg ttgtcgatca gcttcaaggt ggcgatgaac tcggtttcga
     1801 acatcgcccg gcggcggccc tcgtaagcga gggttgcgag gacttcgagc aagtcgaagc
     1861 ggccggtgat cgacccgtag ctggtctcgt ggccgtagat cgccatcagc accgccgggt
     1921 cgaccccgtg gcgcgcctgg atgagctgca ggttgggcca gtgctggtaa tagcggctct
     1981 gtccgcgggc gatcagcacc tcggtcacat gctcgcggcg atagggcgcg aacggcgcca
     2041 tgtagttcga ggtccgcggc cgcggctgct gagcgcggtc gagctcgatc gcccggctgt
     2101 tgagctcaag gtacgggacg gtcgcctgga tggtcgcctc gcgcacgccc ccgtgccgcg
     2161 ccttggccat caggtagatc ttgtaggcgg caaagccggt cggggcggcg taggtctggg
     2221 gcggagcgac ctggaccaac ggcggcgcgg gctgggcctg gggcggcgcc gggcgcggcg
     2281 cgagcgggtc gacctgagcg ctggcgggcc cgaacgcgac cgccgccagg cccaccaatg
     2341 tccagccgct caacacacgc aactcgggcc tccgtccttg ccgaaaccta gaagaaccaa
     2401 cacctctcgc gccagccggg ttcaccgcgc gccttgcacg gcccgccccg gcggcgttag
     2461 gcgtcgctgt ctgaacgaag gatgggtggc cgagcggttt aaggcaccgg tcttgaaaac
     2521 cggcgtgggt gcaagcccac cgtgggttcg aatcccaccc catccgccag cgcggcgacg
     2581 ccttagggca gcgggccaaa aaccatttgt tcaccatttg tcgatcatga cctgatcggg
     2641 ggtagcgctc gaatgagtga ttccgcgaat ccacaggaac gtcccgagcg ccggcgcgcg
     2701 aaccggatcg cgcttcggct cggcgcgacg atgcgcgagc cgaaccgcgg ccgggtcgag
     2761 gcccggctga tcgacatctc gacccacggc tgccgggtgg aggccatttc cggcgcctct
     2821 ccggacagct ggctatggct caac
//
LOCUS       DASTZO010000134         4197 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_767175,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000134 DASTZO010000000
VERSION     DASTZO010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4197)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4197)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4197
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_767175"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFU20_03785"
     CDS             <1..394
                     /locus_tag="VFU20_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU5285619.1"
                     /translation="ALAIARRNAARLGLAERSEFRLGDWGEGLAERFDLILCNPPYVA
                     LTGELGPGVAEHEPHEALFAGDDGLEALRALAPQFPRLLAKGGLAAVEIGFDQAASAA
                     ALLARDGVSARLARDLAGHPRALLITWV"
     gene            622..1296
                     /locus_tag="VFU20_03790"
     CDS             622..1296
                     /locus_tag="VFU20_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4167 domain-containing protein"
                     /protein_id="HEU5285620.1"
                     /translation="MINNRQGGRRRGRGGQRPQNLGGRPDGNRQDNRQRGNAAQLLEK
                     YKSMARDAQLGGDRVQTEYYLQYADHYYRVLGESRARFEEQRRQRGDDMDEDEGDEEL
                     AEAGDEQRSQQPRDDERPGRSDRGERFERSDRAPRYARDRDRDRDGGDGPDRSSEPPR
                     GVVAGIDGPGGANLESIPFDALPPAIGRDSAPEQPEAEEEAPRPKRRARRPRAADDEG
                     DIAPAA"
     gene            1418..2803
                     /locus_tag="VFU20_03795"
     CDS             1418..2803
                     /locus_tag="VFU20_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008827938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HEU5285621.1"
                     /translation="MAAPLAAQPQVPPPSQTAQGDIAVTIYNNNRALIEDKRQLDLPA
                     GRSRQEFRDVSAQIEPATVTLSGRGIGIVEQNFDFDLLSPAALMQKAVGQTVTLVRTN
                     PATGAETRERARILAVNNGVVMQIGDRIEVLRDDGIPARVIFDRVPETLRPRPTLSVT
                     IDADGGGRRPVTLTYLTPGLGWAADYVALFDEANGRMDVQGWITLTNNSGTPYVNAST
                     LLVAGAVGQAGHYRGDPYGRYPPPPPPPPDGMYRPGTETAPRERLGDFYLYPLPERTT
                     IADKQTKQVSFLDVQGTPAARAYEFRNGWLSTADQALSANSVLRFSSARGGGLGDALP
                     AGIMRVYQRDARGNPQFVGEHRIGHTPMGSDIGLATGQAFDVKVRPIVEERTRLSSSR
                     WRTRMRYELTNAGPRPVTVDLVQAGLWGDTRIVDESMKSQRLSADEARWRVAVPANGE
                     ASVTATFDTRH"
     gene            2809..>4197
                     /locus_tag="VFU20_03800"
     CDS             2809..>4197
                     /locus_tag="VFU20_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285622.1"
                     /translation="MRALLTLIALGAAVQAGAQTIAVSPAPDRVAVTVYRDPDRGLEP
                     LNLGWLGGYALVSETRRVRLPPGESELRFEGVTAGIVPQSAIVTGLGEAVLEKNRDAR
                     LLSPGALLDAYLGERLTLRRTSRATGQVRVHDAVVRATADGIVLQTESGFEALRCTGL
                     SETLIAPAVPAGLSAKPTLSVRVRSPQAIERDVTLSYITNNFDWQADYVGDLSADGRS
                     LRLFAWLTLGNSDDTGLADASTQAVAGKLNRERVWVDRGEARPIMLNCWPAGTTSDIP
                     SHASPVTQISSEDIVVTGSRIPPPAPPPPPASPERGGEVMAKEERLGDVRLYRVPIEV
                     TVAARSQKQIALLRRPSVKFESVLRLRPHQGPLEAPLERVLVTRNRPAEGLGLPLPAG
                     KLALFGTRQGRRVLLGEGSIDDHTIGEKVEIRVATVTGVRATQVHERESTGEYRLTLT
                     NDSREAQTAEIEL"
BASE COUNT          660 a         1540 c         1430 g          567 t
ORIGIN      
        1 ggcgctggcg atcgcccggc gcaacgcggc gcggctcgga ctcgccgagc gctccgagtt
       61 ccgcctcggc gactggggcg aggggctcgc cgagcgcttc gacctcatcc tgtgcaaccc
      121 gccctacgtc gcccttacgg gcgagctcgg cccgggcgtc gccgagcacg agccgcacga
      181 ggccctgttc gccggcgacg acgggctcga ggcgttgcgg gcgctcgcgc cgcaattccc
      241 gcggctgctt gccaagggcg gtctcgcggc ggtcgagatc ggcttcgacc aggccgcgag
      301 cgcggccgca ttgctcgcac gcgacggtgt ttctgcgcgc cttgcccgag acttagccgg
      361 acatccccgc gccttgctga taacctgggt ttgaacgaaa tcgcttggca accggccgtt
      421 acggcactac atccttgttc gagggtcggg cccgcatcac cacaaaagtt gagcgcatcc
      481 cgtcccaacc cccgacgcaa tcggccgttc gctaaagcgc tcaagcgcag accggaacga
      541 aagggtcaga gccgcgcagc tgcgccggcc tgcccgcctg aagcgaaaag gggcaagctt
      601 ctgctttgag ggaaagcgca tttgatcaat aatcgacagg gtggccgccg ccgcggccgc
      661 gggggccaga ggccacagaa tctcggcggc aggccggacg gcaaccgcca ggacaatcgc
      721 cagcgcggca acgcggcgca gcttctggaa aaatacaaga gcatggcgcg cgacgcgcag
      781 ctcggcggcg accgcgtgca gaccgaatat tatctgcaat atgccgacca ttattaccgg
      841 gtgctcggcg aaagccgcgc ccgcttcgag gaacagcgcc ggcaacgcgg cgacgatatg
      901 gacgaggacg agggcgacga ggagctggcc gaggccggcg acgagcagcg ctcgcaacag
      961 ccgcgcgacg acgagcggcc gggtcggtcc gaccgcggcg agcgcttcga gcgtagcgat
     1021 cgcgcaccgc gttatgcgcg cgaccgcgac cgcgaccgcg acggcggcga cgggccggac
     1081 cgctcgagcg agccgccgcg cggagtcgtc gccggcatcg acggcccggg cggagccaat
     1141 ctcgaaagca ttccgttcga cgctcttccg cccgccatcg gccgcgattc cgcgcccgag
     1201 cagccggaag ccgaagagga agccccgcgg cccaagcgcc gggcccgccg gccccgcgcc
     1261 gccgacgacg aaggcgacat cgcccccgcc gcctgacccg cgctcggctg ggttttcagc
     1321 cattcaaggt tcaggatcgc tcgcacactt tcccgcggct cgagttgcgg ggagggtgcc
     1381 aatgcgttcg ttgattctgt tgtcgtcggc catcgcgatg gccgctccgc ttgccgccca
     1441 gccgcaggtc ccgccgccgt cgcagaccgc ccagggcgac atcgcggtca ccatctacaa
     1501 caacaatcgc gcgctcatcg aggacaagcg ccagctcgac cttccggccg gccgcagccg
     1561 ccaggagttc cgcgacgtct cggcgcaaat cgagccggcg acggtcaccc tgagcggccg
     1621 agggatcggc atcgtcgagc agaatttcga cttcgacctg ctgtcgcccg cggcgctgat
     1681 gcagaaagcg gtcggccaga cggtgacgct ggtccgaacc aaccccgcga ccggcgccga
     1741 gactcgcgag cgcgcccgga tcctcgccgt caacaatggc gtggtgatgc agatcggcga
     1801 tcgcatcgag gtgctccgcg acgacggcat cccggcccgc gtgatcttcg accgggtgcc
     1861 cgaaacgctg cgcccccgcc cgaccctgtc ggtgaccatc gacgccgacg gcggcggccg
     1921 gcgcccggtc accctcacct atctgacccc gggcctcggc tgggcggccg attatgtcgc
     1981 tctgttcgac gaagccaacg gccggatgga cgtccagggc tggatcactc tgaccaacaa
     2041 cagcggcacg ccctacgtca acgcctcgac cctgctggtc gcgggagcgg tcggccaggc
     2101 cggtcattat cgcggcgatc cctatggccg ctatccgccg ccgccgcccc cgccgccgga
     2161 tgggatgtac cgcccgggca ccgagaccgc gccgcgcgag cgtctcggcg atttctattt
     2221 gtacccgctg cccgagcgga cgacgatcgc cgacaagcag accaagcagg tcagcttcct
     2281 cgacgtccag ggcacgccgg cggcgcgcgc ctacgagttc cgcaacggct ggctgtcgac
     2341 cgccgaccag gcgctgagcg ccaacagcgt gctgcgcttc tcgagcgcgc gcggcggcgg
     2401 cctcggcgac gcgctcccgg ccggaatcat gcgcgtctac cagcgcgacg cgcgcggtaa
     2461 cccgcagttc gtcggcgagc accgcatcgg ccacacgccg atgggctccg acatcggcct
     2521 cgccaccggc caggcgttcg acgtcaaggt gcgcccgatc gtcgaggagc gcactcggct
     2581 ttccagcagc cgctggcgca cccggatgcg ctacgagctg accaacgccg ggcccaggcc
     2641 ggtgaccgtc gacctcgtcc aggccgggct gtggggcgac acacggatcg tcgatgaaag
     2701 catgaagtcg cagcgcttga gcgccgacga ggcgcgctgg cgggtcgcgg tcccggccaa
     2761 cggcgaggcg agcgtcaccg caaccttcga cacccgccac taggcttgat gcgcgcgctg
     2821 ctcacgctga ttgcgcttgg cgcggccgtg caggccggcg cccagacgat cgccgtctcg
     2881 cctgcgcccg accgggtcgc ggtcaccgtc tatcgcgatc ccgaccgcgg gctggagccg
     2941 ctcaacctcg gctggctcgg cggctatgcg ctggtcagcg agacccggcg ggtgcgcctg
     3001 ccgccggggg aaagcgagct gcgcttcgag ggcgtcaccg ccggcatcgt cccgcaaagc
     3061 gcgatcgtca ccggcctcgg cgaagcggtg ctcgagaaga accgcgatgc ccggctgctg
     3121 tcgcccggag cgctgctcga cgcttacctc ggcgagcggc tgaccctgcg ccggacgtcg
     3181 cgcgcgaccg gccaggtgcg ggtccacgac gcggtcgtcc gcgccaccgc cgacggcatc
     3241 gttctgcaga ccgagtccgg gttcgaggcg ctgcgctgca ccggcctgtc cgaaactctg
     3301 atcgccccgg ccgtgcccgc cggcctgtcg gccaaaccga ccctgtcggt gcgcgtgcgc
     3361 agcccgcagg ccatcgagcg cgacgtgacc ctgtcctaca tcaccaacaa tttcgactgg
     3421 caggcggatt atgtcggcga cctgtcggcc gatggccgat cgcttcgctt gtttgcgtgg
     3481 ctaacgctcg gcaacagcga cgacaccggc ctggccgatg cgagcaccca ggccgtggcc
     3541 ggcaagctca accgggagcg cgtctgggtc gaccgcggcg aggccaggcc gatcatgctc
     3601 aattgctggc cggcggggac caccagcgac atcccctctc acgcgagtcc cgtgactcaa
     3661 atatcctccg aggatatcgt cgtcaccggc agccgcattc cgccaccggc cccaccgccg
     3721 ccgccggcgt cgccggagcg cggcggcgag gtgatggcga aggaggagcg gctcggcgac
     3781 gtccggctct accgggtgcc gatcgaggtg accgtcgcgg cgcgctcgca aaagcagatc
     3841 gcgctgctcc gccgtccgag cgtcaagttc gagagcgtgc ttcggctgcg cccgcaccag
     3901 gggccgctcg aagcgccgct ggagcgggtc ctggtgaccc gcaaccgccc ggccgagggg
     3961 ctcggtctgc cgctgccggc gggcaagctg gctttgttcg gcacccgcca gggccgccgc
     4021 gtgctgctcg gcgaaggcag catcgacgac catacgatcg gcgagaaggt cgagatccgg
     4081 gtcgccaccg tcaccggggt ccgggcgacc caggtccacg agcgtgagtc gaccggcgag
     4141 tatcggctga ccctgaccaa cgattcgcgc gaggcccaga ccgccgagat cgagctt
//
LOCUS       DASTZO010000135         7239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1134236,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000135 DASTZO010000000
VERSION     DASTZO010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1134236"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1570)
                     /gene="lptD"
                     /locus_tag="VFU20_03805"
     CDS             complement(<1..1570)
                     /gene="lptD"
                     /locus_tag="VFU20_03805"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HEU5285623.1"
                     /translation="MVWWSTLPLMLGLPASALAQPAGPDRSPEAAGAAEAGDVVRFSA
                     DQVSYDSANELVTASGAVRMSREGNYLAADRVTWDRRTGEVRAQGNVVVVNPEGDKLV
                     GDSVILTDSLRDGSIENLLVVLESGGRIAAARGTRTGDVTALDNAIYSPCPVTTDSGC
                     PRNPSWSITAARVIHDPARSQVRFQGGRLRLFGVTLPLLPIFSIGTAAQGGGVSGWLV
                     PDISVSSRNGLELAMPYYRRFAPNRDLTLTPHLYTESGPALEARYRHLDRIGAFQVGA
                     FATYSKIDDPDPDDPTPDTRNGFRAYFEGNGKAQFNPLWSLTGSVRVATDKTVTQRYD
                     ISRDDRLRSFLNAERIAPTSYVSIAGWAFQGLRVDDVQRRIPIALPAIDARFRLDPPL
                     IGGHVELQANSLSILRIDGQDTQRAFVSGRWDLRRLTNWGHQLLFTAFTRGDVYHTVE
                     SETTEVPIYRGTEGWHTRAIAALAAEVQWPLVGPAFGGLQRLVPRLQLVLTPPTPNLA
                     IPNEDARAVDLEDSN"
     gene            1749..3218
                     /locus_tag="VFU20_03810"
     CDS             1749..3218
                     /locus_tag="VFU20_03810"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEU5285624.1"
                     /translation="MKIRFADRLPDGDYALVVPVAGKDRAALERLGADRAGVEAALTR
                     QRFEGEAATSAEHYLAAKDGVRRLLAVGTGNGSAPADAAEKLGGTVAARLLTSGEKHA
                     VIDLSGLGYDADAAAKVALGASLRGWRYDRYRTRLKEKQKPTLAELTIVGGGADAKAR
                     WEQRWQPVLEGVKLTRELVTEPANIIYPESFVERCRHLASLGIELEVLDGAAMKKLGM
                     GALLGVAQGSVRDPRLLVLRWNGGKKGVPPLAFVGKGVTFDTGGISIKPAQGMEAMKW
                     DMGGAAAVAGAFKALALRKAKANVVGVCGMVENMPSGTAQRPGDVVTTMSGQTVEVIN
                     TDAEGRLVLADAIAYTQRNFKPATVIDLATLTGAIVLSLGHEFGGLFTPDDQLAGQLL
                     AAAQASGDRLWRMPLDENFDRLIDSPIADMKNVGPREGAAITAAEFIHRFVDEGVRWA
                     HLDIAGMAWSDKAGTTYDKGATGFGVRLIDQFVADTAEA"
     gene            3241..3678
                     /locus_tag="VFU20_03815"
     CDS             3241..3678
                     /locus_tag="VFU20_03815"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HEU5285625.1"
                     /translation="MQVDFYQLGQTSPEKVIAALAEKLLAEDARLLIVAEDETLLARL
                     DRLLWDQGPASFLPHGLAGGADDVRQPILLSTSPDAPNLARNMLIADGQWREAALSYD
                     RAFYLFDDDSLEGARLAWKLLAGRDGVERNFWAREEGRWVHKA"
     gene            3756..4178
                     /gene="ndk"
                     /locus_tag="VFU20_03820"
     CDS             3756..4178
                     /gene="ndk"
                     /locus_tag="VFU20_03820"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU5285626.1"
                     /translation="MATERTFSIIKPDATRRNLTGAITKMLEEGGLRVIASKRIRMTR
                     DQAEGFYAVHRERPFYNDLCTFMTSGPVVVQVLEGDNAVQRNREVMGATNPADADEGT
                     IRKTYAESIEANSVHGSDSPENAKIEIDFFFKPEEIVG"
     gene            complement(4175..4678)
                     /locus_tag="VFU20_03825"
     CDS             complement(4175..4678)
                     /locus_tag="VFU20_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285627.1"
                     /translation="MSDSDSRSRRRGIALGEIISLAALIVSALGLWITWQSTGEDRPT
                     RVVEQRQPIPLTLQGAADRDGRELVLTPAEPGHALVSLTLTIRGSPPIEVASDGRLDS
                     NAVEAAVKDTDRDRKRAGKIPVRIAARYVEAGAERRGGGTYVLRYRWEGGGLFGGRSL
                     RLVGLSR"
     gene            complement(4675..5040)
                     /locus_tag="VFU20_03830"
     CDS             complement(4675..5040)
                     /locus_tag="VFU20_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEU5285628.1"
                     /translation="MNEDTLNRIRTAALSLPETEERLSHGQPTFFVAGKQFAQLRENH
                     HGDGRTVVCVRTSGLDEQAMLIESDPETYSRPAYLGPSGWVSVNLAGGGVDWDHVRDR
                     IAVSWELAAPRRLLEAGGR"
     gene            complement(5037..5612)
                     /gene="purN"
                     /locus_tag="VFU20_03835"
     CDS             complement(5037..5612)
                     /gene="purN"
                     /locus_tag="VFU20_03835"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU5285629.1"
                     /translation="MADPEKGDRRRVAILISGRGSNMRALIEQADGYQVVLVASNKPQ
                     AAGLDFARGAGIATWAWDSKGVPRGEFEAALDRALRDHRVGTVALAGYMRLLSPAFVE
                     RWRGRMVNIHPSLLPRYPGLDTHARAIAAGDSVAGCSVHIVTEEVDAGQVLGQAEVPI
                     EPGDDAQTLEQRVLEAEHRLYPAVLREFVRR"
     gene            complement(5605..6690)
                     /gene="purM"
                     /locus_tag="VFU20_03840"
     CDS             complement(5605..6690)
                     /gene="purM"
                     /locus_tag="VFU20_03840"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU5285630.1"
                     /translation="MSRKPLTYADAGVSIAAGNALVKAIGPLARSTARSGADAQLGGF
                     GGLFDLKAAGYRDPLLVAANDGVGTKLKLAIELGRHGGVGIDLVAMCANDLVVQGAEP
                     LFFLDYFATGKLDGEVAAAVVESIADGCRQAGCALIGGETAEMPGMYAESDYDLAGFC
                     VGAVERSELLTGSAIAPGHLILGLASSGVHSNGFSLVRRIIAEREWDLNQSLPSGQTL
                     GEALLEPTRIYVAALLPVVRSGRIAGLAHITGGGFLENIPRVLPQDCHAVVEAGDWPL
                     PRIFALLQEGGAIAAAEMARTFNCGIGMAAIVAEADADAVARELAEAGETVHPIGRIT
                     SGKRGCTVRGAAGSWGSDEDWTATFHG"
     gene            6754..>7239
                     /locus_tag="VFU20_03845"
     CDS             6754..>7239
                     /locus_tag="VFU20_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HEU5285631.1"
                     /translation="MPRRTFLPALLLFLLAAALSGTLYAQLESGERGILPIDSSGTLE
                     ISGIHVDVGGKDAASARFAGWRLAQRQGFRALWAKTHGRPIGEAPSLSDSVLDGLVSS
                     IIVEREQIGPNRYIADLGILFDRARAGEMLGVGGERRQSPPMLLIPITVSGGTATSVE
                     LR"
BASE COUNT         1080 a         2572 c         2525 g         1062 t
ORIGIN      
        1 aattgctgtc ctcgaggtcg acggcgcgcg cgtcctcgtt ggggatggcg aggttggggg
       61 tcggcggggt cagcaccagc tggagcctcg gcaccaggcg ctggaggccg ccgaacgcgg
      121 gcccgaccaa tggccactgg acctcggccg cgagcgccgc gatggcgcgc gtgtgccagc
      181 cctcggtgcc gcggtagatc ggcacttcgg tcgtttcgga ttcgaccgtg tggtagacgt
      241 cgccgcgggt gaaggcggtg aacagcagtt ggtgccccca gttggtcagc cgccgcaggt
      301 cccagcggcc gctgacgaac gcgcgctggg tgtcctggcc gtcgatcctg aggatcgaga
      361 ggctgttggc ctggagctcg acatggccgc cgatcagcgg cggatcgagc cggaagcgcg
      421 cgtcgatcgc cggcagagcg atcgggatcc gccgctggac atcgtcgacg cgcagcccct
      481 ggaacgccca gccggcgatg ctgacgtacg aggtgggcgc gatccgctcg gcgttcagga
      541 agcttcgcag ccggtcgtcg cggctgatgt cgtagcgctg ggtgaccgtc ttgtcggtcg
      601 ccacccggac cgagccggtc aggctccaca agggattgaa ctgggccttg ccgttgccct
      661 cgaaataggc gcggaagccg ttgcgggtgt cgggcgtcgg gtcgtcgggg tcggggtcgt
      721 cgatcttgct gtaggtcgcg aatgccccga cctggaacgc tccgatccgg tcgaggtggc
      781 ggtagcgggc ctcgagcgcc ggcccgcttt cggtgtacag gtgcggggtc aaagtgaggt
      841 cgcggttggg ggcgaagcgc cggtagtagg gcatcgcgag ctcgagcccg ttgcgcgacg
      901 agacgctgat gtcgggcacc agccagccgc tgaccccgcc gccctgggcc gcggtgccga
      961 tgctgaagat cggcagcagc ggcagggtca ccccgaacag ccgcagccgc ccgccctgga
     1021 agcggacctg gctccgcgcc gggtcgtgga tcacccgggc cgcggtgatc gaccagctcg
     1081 ggttgcgcgg gcagccgctg tcggtggtca ccgggcacgg cgagtagatc gcattgtcga
     1141 gcgcggtgac gtcgcccgtg cgggtgccgc gcgccgcggc gatccggccg ccgctttcga
     1201 gcacgaccag cagattctcg atgctcccgt cgcgcaggct gtcggtgagg atgacgctgt
     1261 cgccgacgag cttgtcgccc tcgggattga cgacgacgac attgccctgc gcccggacct
     1321 cgccggtgcg gcggtcccag gtcacccggt cggcggcgag gtaattgccc tcgcgcgaca
     1381 tgcgcaccgc gcccgacgcg gtgaccagct cgttggcgct gtcgtagctg acctgatcgg
     1441 cgctgaagcg gacgacatcg cccgcctcgg ccgctccggc cgcttcgggc gaccggtcgg
     1501 gcccggcggg ctgggcgagg gcggacgccg gcagcccgag catcagcggc agcgtcgacc
     1561 accagaccaa tcgtctacgc aaacctgtcc ccgccgctca cctggacttg cctggtgccg
     1621 ctatcgcacc tcgggcggcg cgcgacaatc cgattggctc atcgccggcc cggcttcgcc
     1681 ccgctttgcc tttcggcagc cgcgtcctaa aggcccccaa cccctttttc cgccaaggac
     1741 cgaccttcat gaagattcgt ttcgccgacc gccttcccga cggagactat gcgctggtgg
     1801 tgccggtcgc cggcaaggac cgcgccgcgc tcgagcggct cggcgccgac cgcgccgggg
     1861 tcgaggcggc gctcacccgc cagcgcttcg agggcgaagc ggcgacgtcg gccgagcatt
     1921 atctggccgc gaaggacggc gttcgccggc tgctggcggt cggcaccggc aacggctcgg
     1981 cccccgccga cgccgccgaa aagctcggcg ggacggtcgc cgcgcggctc ctgacctccg
     2041 gggagaagca cgcggtcatc gatttgtccg ggctcggcta cgatgccgac gcggccgcga
     2101 aggtcgcgct cggggcgtcg ctccgcggct ggcgctacga ccgctaccgg acccgcctca
     2161 aggagaagca gaagccgacc ctggcggagc tcaccatcgt cggcggcgga gcggacgcca
     2221 aggcccgctg ggagcagcgc tggcagccgg tcctggaagg cgtcaagctg acccgcgagc
     2281 tggtcaccga gccggccaac atcatctatc ccgaaagctt cgtcgagcgc tgccgccacc
     2341 tggccagcct cgggatcgag ctcgaggtgc tcgacggagc ggcgatgaag aagctgggca
     2401 tgggggcgct gctcggcgtc gcccaaggct cggtgcgcga cccgcggctg ctggtgctgc
     2461 gctggaacgg cggcaagaag ggcgtgccgc cgctcgcctt cgtcggcaag ggcgtgactt
     2521 tcgacaccgg cggcatctcg atcaagccgg cccagggcat ggaagcgatg aagtgggaca
     2581 tgggcggcgc cgcggcggtc gcgggcgcgt tcaaggcgct cgcgctgcgc aaggcgaagg
     2641 ccaatgtcgt cggggtctgc ggcatggtcg agaacatgcc gtcgggcacc gcccagcgcc
     2701 cgggcgacgt cgtcaccacc atgtccggcc agacggtcga ggtgatcaac accgacgccg
     2761 aagggcgcct cgtcctcgcc gacgcgatcg cctacaccca gcgcaacttc aagccggcga
     2821 cggtgatcga cctcgcgacc ctgaccgggg cgatcgtgct cagcctcggc cacgagttcg
     2881 gcggcctgtt cacgcccgac gaccagctcg cgggccagtt gctcgccgcc gcccaggcat
     2941 cgggcgaccg gctgtggcgg atgccgctcg acgagaattt cgaccggctg atcgattcgc
     3001 cgatcgccga catgaagaac gtcggcccgc gcgaaggcgc cgcgatcacc gccgccgagt
     3061 tcatccaccg cttcgtcgac gagggcgtcc gctgggcgca tctcgacatc gccggcatgg
     3121 cctggtcgga caaggccggc accacctacg acaagggcgc gaccggcttc ggtgtgcggc
     3181 tgatcgacca gttcgtcgcc gacaccgccg aggcctgacc gccccgtgcc ggcctgaccg
     3241 atgcaggtcg atttctacca gctcgggcaa acgtcgcctg aaaaggtgat cgccgccctc
     3301 gccgagaaac tgctggccga ggacgcccgc ctgctgatcg tcgccgagga cgagactctg
     3361 cttgcccgcc tggaccgcct gctgtgggac caggggccgg cgagcttcct tccgcacggc
     3421 ctcgccggcg gcgccgacga cgttcgccag ccgatcctgc tgtcgaccag ccccgacgcg
     3481 cccaacctcg cgcgcaacat gctgatcgcc gacggccagt ggcgcgaggc ggcattgagc
     3541 tacgaccgcg ctttctatct gttcgacgat gattcgctcg aaggcgcccg gctggcgtgg
     3601 aagctgctcg ctggtcgcga cggggtcgag cgcaacttct gggcccggga ggaaggccgc
     3661 tgggtgcaca aggcctgacc cttgccgcca tcggccggcg cggctaggcg ctgaggcaca
     3721 ttccagccac ctcaattcca ggagaccgct cggccatggc gaccgaacgc actttttcga
     3781 tcatcaagcc cgacgccaca cggcgcaacc tcaccggcgc gatcaccaag atgctcgagg
     3841 agggcgggct ccgggtgatc gcatcgaagc gcattcgaat gacccgcgac caggccgaag
     3901 gcttttacgc ggtccaccgc gagcggccgt tctacaacga tttgtgcacc ttcatgacgt
     3961 cggggccggt ggtcgtgcag gtgctcgagg gcgacaatgc ggtgcagcgc aaccgcgagg
     4021 tgatgggcgc caccaacccg gccgacgccg acgagggcac gatccgcaag acctacgccg
     4081 aatcgatcga ggccaattct gtccacggct ccgacagccc ggagaatgcg aagatcgaga
     4141 tcgacttctt cttcaagccc gaagaaatcg tcggctagcg gctcagcccg accagtcgca
     4201 gcgaccggcc gccgaacagg ccgccgccct cccagcgata gcgcagcaca taggtgccgc
     4261 cgccgcggcg ctcggctccc gcctcgacat agcgggcggc gattcgcacc gggatcttgc
     4321 cggcccgctt gcggtcgcgg tcggtgtcct tgaccgccgc ttcgaccgcg ttcgaatcga
     4381 gccggccatc gctggcgacc tcgatcggcg gcgagccgcg gatcgtcagc gtgagcgaca
     4441 ccagtgcgtg acccggttcg gccggggtca gcacgagctc gcgtccgtcg cgatccgcgg
     4501 ccccctgcag cgtcaaggga atcggttgcc tttgctcgac gacccgggtc ggccggtcct
     4561 ccccggtgct ttgccacgtg atccacaacc caagcgcgga aacgatcagc gcggcgagac
     4621 tgatgatctc gcccaacgcg atgccgcggc ggcgcgaccg ggaatcactg tcgctcatcg
     4681 tccgcctgct tcgagcaacc gccgcggcgc cgcaagctcc cagctgacgg caatccggtc
     4741 gcggacatgg tcccagtcga cgccgccgcc ggcgaggttg accgacaccc agcccgacgg
     4801 gccgagatag gcgggacgcg agtaggtttc gggatccgat tcgatcagca tcgcctgctc
     4861 gtcgaggccg ctggtcctca cgcagaccac ggtcctgccg tcgccgtggt ggttttcgcg
     4921 cagctgggcg aactgcttgc cggcgacgaa gaaggtcggc tggccgtgcg acagccgctc
     4981 ctcggtctcc ggcaggctga gcgccgccgt ccggatccgg ttcaaagtgt cctcgttcat
     5041 cgccggacga actcccgaag caccgccgga tatagccgat gttccgcctc gagcacgcgc
     5101 tgctcgagcg tttgggcgtc gtcgccgggc tcgatcggca cttcggcctg gccgagcact
     5161 tggccggcgt cgacctcctc ggtgacgatg tggaccgagc agccggcgac cgaatccccg
     5221 gcggcgatcg cccgcgcgtg ggtgtcgagg cccggatatc tgggcagcag cgacgggtgg
     5281 atgttgacca tccggccgcg ccagcgctcg acgaaagcgg gcgagagcag gcgcatgtag
     5341 ccggccaggg cgacggtgcc gacgcggtga tcgcgcagcg cccggtcgag cgcggcctcg
     5401 aactcgccgc gcggcacgcc tttgctgtcc caagcccagg tcgcgattcc ggcgccgcgg
     5461 gcgaagtcga ggcccgcggc ctgcggcttg ttcgacgcga ccagcaccac ttgatagccg
     5521 tccgcctgct cgatcagcgc ccgcatgttg ctcccgcggc ccgagatcag gatggcgact
     5581 cgccggcggt cgcccttctc agggtcagcc atggaaggtc gcggtccagt cctcgtccga
     5641 gccccagctg ccggccgcgc cgcggaccgt gcagccccgc ttgcccgagg tgattcggcc
     5701 gatcgggtgg accgtctcgc cggcctccgc cagctcccgc gcaacggcat cggcgtcggc
     5761 ttcggcgacg atcgcggcca tgccgatgcc gcagttgaag gtgcgcgcca tctcggccgc
     5821 ggcgatggcg ccgccttcct ggaggagcgc gaagatgcgc ggcagcggcc agtcgccggc
     5881 ttctaccacc gcgtggcaat cctgcggcag aacccgcgga atattctcga ggaatccgcc
     5941 gccggtgatg tgcgccagcc cggcgatccg gccggaacgg accaccggca ggagcgcggc
     6001 gacatagatc cgggtcggct cgagcagcgc ctcgccgagc gtctgcccgc tcggaagcga
     6061 ctggttgagg tcccattcgc gctcggcgat gatccggcgg acgagcgaaa agccgttgct
     6121 gtgaacgccc gagctcgcaa ggccgaggat caaatgcccg ggcgcgatcg cgctgccggt
     6181 gagcagttcc gagcgctcga ccgcgccgac gcaaaagccg gcgaggtcgt agtcgctctc
     6241 ggcgtacatg cccggcattt cggcggtctc gccgccgatc agcgcgcagc cggcctggcg
     6301 gcacccgtcg gcgatcgact cgacgaccgc cgcggcgacc tctccgtcca gcttgccggt
     6361 cgcgaaatag tcgaggaaga acaaaggctc ggcgccctgg acgacaaggt cgttggcgca
     6421 catcgcgacc aggtcgatgc ccactccgcc gtggcgccca agctcgatcg ccagcttgag
     6481 cttggtgccg acgccgtcgt tggcggcgac cagcagcggg tcccggtagc ccgcggcctt
     6541 gaggtcgaac aggccgccga acccgccgag ctgcgcgtcc gcgccggacc gcgccgtcga
     6601 tcgcgccagc ggcccgattg ccttcaccag cgcattgccg gcggcgatcg acacgcccgc
     6661 gtcggcatag gtcagaggct ttcggctcac ggctgtggcc cgtagagaca agccgcttgg
     6721 atttccacgc cgttgtcgcc aaaagccgcc ccgatgcccc gccggacgtt cctccccgca
     6781 ctgctgctgt tcctgctcgc cgccgcgctg tcgggaacgc tctacgcgca gctcgagagc
     6841 ggcgagcgcg gcatcctgcc gatcgacagc tcgggcacgc tcgagatcag cggcatccac
     6901 gtcgacgtcg gcggcaagga cgcggccagc gcgcgcttcg ccggctggcg cctcgcccag
     6961 cgccagggct tccgggcgct gtgggcgaaa acccacggcc gcccgatcgg cgaggctccg
     7021 tcgctgtcgg attcggtcct cgacgggctg gtcagctcga tcatcgtcga gcgcgagcaa
     7081 atcggcccca accgctacat cgccgatctc ggcatcctgt tcgaccgcgc ccgcgccggc
     7141 gaaatgctcg gagtcggcgg cgagcgccgg caatcgccgc cgatgcttct gatccccatc
     7201 acggtgagcg gcggcaccgc gaccagcgtc gagctgcgc
//
LOCUS       DASTZO010000136         3354 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1108481,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000136 DASTZO010000000
VERSION     DASTZO010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3354)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3354)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3354
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108481"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /locus_tag="VFU20_03850"
     CDS             complement(<1..925)
                     /locus_tag="VFU20_03850"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5285632.1"
                     /translation="MHDVVIIGAGHNGLTCAFYLARKGLKVAVLERATTVGGAAVTDE
                     FLPGFRNSAAAYTVSLLNPRVIRDMALERHGLKVVLRKTDNFLPGDGDYLLSGRQGLT
                     RRELVRHAKGDGEAYDRYLAELGTVVPLLRKWILRAPPEIGGGVGGLPKLLQLARDLV
                     GLSLAEKRILHDYATRSAGDILDRHFEGELAKALFGFDGVVGNFASPYTPGTAYVLLH
                     HLFGEAAGVPGAWGHAIGGMGSITQAMAHSAREAGADILLNTPVEEVIVERDRKGARA
                     AGVVAGGRAWRAKQVVAGVNPKLLFDKMVPRG"
     gene            complement(941..2281)
                     /gene="gorA"
                     /locus_tag="VFU20_03855"
     CDS             complement(941..2281)
                     /gene="gorA"
                     /locus_tag="VFU20_03855"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEU5285633.1"
                     /translation="MADFDLFVIGAGSGGVRAARVAAAHGARVAIAEEHKVGGTCVIR
                     GCVPKKLLVYGAHFAEDIDDAAMFGWDVPEKRFDWAVLRDNVLAEVSRLEAAYTDTLT
                     NHDVQIFHERAELAGANSVRLASGKTISAGKILLATGARPLMPEIPGIEHAISSNEVF
                     HLEELPKRVVIAGGGYIANEFAGIFHQFGSHVTLVNRSETMLRHYDQQLVDRLLQISV
                     SKGIHFEFNVTFKCIERRGDGSLRLVMNGCDDMEANQVLFAIGRQPNVEGLGCPEAGV
                     EVGERGEIKVDADNRTSVPSIFAIGDVTDRVQLTPVAIREGQAFADTFFGNKPTRVDY
                     ACIPSAVFSHPPLAGVGMTEGQARNQLGAVKAYTADFRPMKNVLAGRSERSLYKLVVD
                     GHSDRIVGVHMIGPDAPEILQAAAIAVKAKLKKADFDAVIALHPTMAEELVLMR"
     gene            complement(2281..>3354)
                     /locus_tag="VFU20_03860"
     CDS             complement(2281..>3354)
                     /locus_tag="VFU20_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU5285634.1"
                     /translation="LVAALGRRRAGYKVRFLSNIDGEAFDEAVGELDPATTLVVVASK
                     TFTTTETLANMGAALRWLAAAGVEDPHGRLIAVTARPKAAVEAGIDETRVLAFAQGVG
                     GRYSLWSSVGVSAALALGWDVFEELLEGAAEMDRHFRFAEPARNGPLVAAFADRLYAR
                     RGWQTRALFAYDERLRLLPAYLQQLEMESNGKSVTADGKPVDQESAPVTWGGTGTDAQ
                     HAVFQLLHQGTVRVPVEFVAAAEAEHSQADEHHRLLLLNAFAQGAALMQGRESDDPQR
                     NYPGDRPSATVLLERLDARSLGALIAFYEHRTFANAVLLGVNPFDQFGVELGKEIARE
                     LGDGADDGALDPSTRALIERAGV"
BASE COUNT          504 a         1218 c         1104 g          528 t
ORIGIN      
        1 ctccgcgcgg caccattttg tcgaacagca gcttcgggtt gaccccggcg accacctgct
       61 tcgcgcgcca cgcccgcccg cccgcgacga cgcccgcggc gcgtgcgccc ttgcgatcgc
      121 gctcgacgat gacttcctcg accggcgtgt tgagcaggat gtcggcgccg gcctcgcgcg
      181 ccgagtgcgc catcgcctgg gtgatcgatc ccatgccgcc gatcgcgtgg ccccacgcgc
      241 ccggcactcc ggccgcctcg ccgaacaaat ggtggagcag cacataggcc gtgcccggcg
      301 tgtaggggga ggcgaaattg ccgaccacgc cgtcgaaccc gaacagggct ttcgccagct
      361 cgccctcgaa gtggcggtcg aggatgtcgc cggcgctgcg ggtcgcgtaa tcgtgcagga
      421 tccgcttttc cgcgagcgac agcccgacca ggtcgcgcgc cagctgcagc agcttgggca
      481 ggccgccgac cccgccgccg atctcgggcg gggcgcgtag gatccacttt cggagcagcg
      541 gcaccaccgt cccgagctcg gcgagatagc ggtcgtaggc ctcgccgtcg cccttcgcgt
      601 ggcgcaccag ctccctgcgg gtcagcccct ggcggccgga cagcagataa tcgccgtcgc
      661 cgggcaggaa gttgtcggtc ttgcgcagca cgaccttcag cccgtgccgc tcgagcgcca
      721 tgtcgcggat caccctgggg ttgagcaggc tgacggtgta ggccgccgcg gagttcctga
      781 agccgggcag gaactcgtcg gtgacggccg ctccgccgac cgtcgtcgcg cgctcgagca
      841 ccgcgacctt gaggcccttc ctggcgaggt agaaggcgca ggtcaggccg ttgtggccgg
      901 cgccgatgat gacgacgtca tgcatgtcga aagcctggca tcagcgcatc agcaccagtt
      961 cttcggccat cgtcgggtga agcgcgatca ccgcgtcgaa atcggctttc ttgagcttgg
     1021 ccttcaccgc gatcgccgcc gcctgcagga tttccggggc gtcggggccg atcatgtgca
     1081 cgccgacgat gcggtcgctg tggccgtcga ccaccaattt gtacagcgag cgctcgctgc
     1141 ggccggcgag cacgttcttc atcggccgga agtcggcggt gtaggccttg accgcgccca
     1201 attggttgcg cgcctgccct tccgtcatcc cgaccccggc cagcggcggg tggctgaaca
     1261 ccgcggacgg gatgcacgca taatcgaccc gcgtcggctt gttgccgaag aaggtgtcgg
     1321 cgaacgcctg gccctcgcgg atcgcgacgg gggtcagctg gacccggtcg gtgacgtcgc
     1381 cgatggcgaa gatcgacggc accgacgtgc ggttgtccgc atcgaccttg atttcgccac
     1441 gctcgccgac ctcgaccccc gcttccgggc agccgagccc ctcgacattg ggctggcggc
     1501 cgatcgcaaa cagcacctgg ttcgcctcca tgtcatcgca gccgttcatc accagccgga
     1561 gcgacccgtc gccgcgcctt tcgatgcact tgaaagtcac gttgaactcg aaatggatgc
     1621 ccttggacac cgagatctgg agcagccggt cgaccagctg ctggtcgtag tggcgaagca
     1681 tggtctcgga ccggttgacc agggtcacat ggctcccgaa ctggtggaag atgccggcga
     1741 actcgttggc gatatagccg ccgccggcga tcaccacccg cttcggcaat tcctcgaggt
     1801 ggaacacctc gttggagctg atcgcatgct cgatcccggg gatctcgggc atcagcgggc
     1861 gcgctccggt cgcgagcagg atcttgccgg cactgatcgt cttccccgac gcgagccgca
     1921 ccgaattggc gcccgcaagc tcggcgcgct cgtggaaaat ctgcacgtcg tggttggtca
     1981 acgtgtcggt gtaggccgcc tcgagccgcg acacctcggc gagcacattg tccctcagca
     2041 ccgcccagtc gaagcgcttc tcggggacgt cccagccaaa catcgccgcg tcgtcgatgt
     2101 cctcggcgaa gtgggcgccg tagaccagca gcttcttggg cacgcatccg cggatcacgc
     2161 aggtgccgcc gaccttgtgc tcctcggcga tcgccaccct ggcgccgtgc gcggccgcga
     2221 cccgcgccgc gcgcaccccg cccgatccgg cgccgatcac gaacaggtcg aagtcggcca
     2281 tcagacgccc gccctttcga tcagagcgcg ggtcgacgga tcgagcgcgc cgtcgtccgc
     2341 gccatcgccg agctcgcgcg cgatttcctt gccaagctcg accccgaact ggtcgaacgg
     2401 gtttactccg agcagcaccg cattggcgaa cgtccggtgc tcgtagaaag cgatcaacgc
     2461 gcccagcgac cgcgcgtcca gccgctcgag cagcaccgtc gcgcttggcc ggtcgcccgg
     2521 ataattgcgc tgcgggtcgt cgctttcccg accctgcatc aacgccgcgc cctgcgcgaa
     2581 cgcgttcaga agcagcagcc ggtgatgctc gtcggcctgc gaatgctcgg cctccgcggc
     2641 ggcgacgaac tcgaccggca ccctgaccgt gccctggtga agcagctgga acaccgcgtg
     2701 ctgcgcgtcg gtgccggtcc cgccccaggt caccggcgcg ctttcctgat cgaccggttt
     2761 tccatccgcc gtgaccgact tgccgttcga ttccatctcg agctgctgga gataggccgg
     2821 cagcagccga agccgctcgt cgtaggcgaa cagggcgcgc gtctgccagc cccggcgcgc
     2881 gtagagccgg tcggcgaacg ccgcgaccag cggcccattg cgcgccggct cagcgaagcg
     2941 gaagtggcgg tccatctcgg ccgctccctc gagcagctcc tcgaacacgt cccagcccag
     3001 cgccagcgcc gccgacaccc cgaccgagct ccacagcgaa tagcggccgc cgaccccctg
     3061 cgcgaaggcc agcacgcggg tctcgtcgat ccccgcctcg accgccgctt tgggcctcgc
     3121 ggtgacggcg atcagccgcc cgtgcgggtc ctcgaccccc gctgcggcga gccagcgcag
     3181 cgccgcgccc atgttggcca gggtctcggt ggtggtgaag gtctttgagg cgaccacgac
     3241 cagggtcgtc gccgggtcga gctcgccgac cgcctcatcg aaggcctcgc cgtcgatgtt
     3301 cgagaggaac cggaccttgt agccggcgcg ccgccggccg agcgcagcga ccag
//
LOCUS       DASTZO010000137         3577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_793694,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000137 DASTZO010000000
VERSION     DASTZO010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3577
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793694"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..1152
                     /locus_tag="VFU20_03865"
     CDS             4..1152
                     /locus_tag="VFU20_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEU5285635.1"
                     /translation="MQPIATAEASGLVPLKSEEVSELDKKVSQFVEELAALDSNSPDF
                     GKKVDALTAMGRKEIAEAAGASNRFLDRPVRAIDADTGIGADLTELRRTVEDLDPARH
                     GKLMQKKKLFGIIPWGNRLRNYFDSYKSSQSHISAILARLASGKDELLMDNAAIDTER
                     AGLWKTMHKLEQMIHISRSLDKSLEDKANELDATEPAKAKAIRETALFYTRQRTTDLL
                     TQMAVTVQGYLALDLVKKNNVELVKGVDRASTTTVSALRTAVTVAQAMTNQRLVLEQI
                     GALNTTTAGMIDSTGELLRTQTGAIHEQAASSTIPLETLQRAFQNIYETMDQIDRFKL
                     EALGNMKQTVETLSTEVEKSKGYIARAEGVEQGKLKGAASPFTPIESK"
     gene            1149..1874
                     /locus_tag="VFU20_03870"
     CDS             1149..1874
                     /locus_tag="VFU20_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285636.1"
                     /translation="MNTDRYNQVIARADRVMKALDERDGPVRAAARRERQRLNADLGR
                     RLARVGVGVGLVSIATIVIGLIVPIGMFGFLAAVGLAIGIAAMLAVIPSQARSAAAPA
                     ADLPNGEMVQRFDSYIYRSRAALPAPAQSELDAISAALPSLRQTLERVDALDPNAQDA
                     RRLMSLHLPGLIDRYRQVPHAYRNERDGEGISVDERLVEGLAAGRAALAEISEKLARS
                     DMIAFETQGRFIKSRYGEERIDQ"
     gene            complement(1878..3077)
                     /locus_tag="VFU20_03875"
     CDS             complement(1878..3077)
                     /locus_tag="VFU20_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HEU5285637.1"
                     /translation="MTAPLAGIRVLDLSRVLAGPWCTQLLGDLGADVIKIERPGAGDD
                     TRHWGPPWHGEGEGRVAAYFLAANRGKRSAAIDFATGEGAELVRRLAARCDVVVENFK
                     VGALARFGLDAESLRRENPRLIVASITGFGQDGPYADRAGYDYIIQGMGGLMSVTGRP
                     DGEPGGGPMRVGVAVADLFTGLYTAVAILAALHRREKTGEGAHIDMALLDVQLAMLAN
                     QASNALVSGEDPPRQGNTHPNIVPYQPFEAADRPIVIAIGNDRQFARLAELCCHPEWA
                     SDERFATNGARVANRDEIVRLIAQCIARKPAAEWFDKLESAGIPAGPINRVGQALADV
                     QAQHRAMVRTIGEMPLVGSPVRIDGARADSDLPPPALGEHTGAVLGELGVSGEEFERL
                     KADGVVG"
     gene            complement(3074..>3577)
                     /locus_tag="VFU20_03880"
     CDS             complement(3074..>3577)
                     /locus_tag="VFU20_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5285638.1"
                     /translation="DRAFVDASRDGWAQKPIIEMLIPSTLDPGLAPRGKHVASLFCQH
                     FRFDLGPGRSWDHEREAAADAIIATVDSHAPGFAQSLLGRQIHSPLDLERRFGLVGGD
                     IFHGKMGLDQLFSARPMIGAADYRMPLKGLYLCGSGAHPGGGVTGAPGHNAARAVLAD
                     RRPWKRA"
BASE COUNT          580 a         1292 c         1218 g          487 t
ORIGIN      
        1 gccctgcagc cgatcgccac ggccgaggcg tcgggcctcg tcccgctcaa gagcgaggag
       61 gtcagcgagc tcgacaagaa ggtgtcccag ttcgtcgagg agctggccgc gctcgacagc
      121 aacagccccg atttcggcaa aaaggtcgac gccctgacgg cgatggggcg caaggagatc
      181 gccgaggcgg cgggcgcgtc caaccgcttc ctcgaccggc cggtgcgggc gatcgacgcc
      241 gacaccggca tcggcgccga cctgaccgag ctccgccgca ccgtcgagga cctcgacccg
      301 gcccggcacg gcaagctgat gcagaagaag aagctgttcg ggatcatccc gtggggcaac
      361 cggctgcgca attatttcga cagctacaag tcgagccaga gccacatctc ggcgatcctc
      421 gcccgactcg cctcgggcaa ggacgagctg ctgatggaca atgccgcgat cgacaccgag
      481 cgcgccggcc tgtggaagac gatgcacaag ctcgagcaga tgatccacat ctcgcggagc
      541 ctcgacaaga gccttgagga caaggccaac gagctcgacg cgaccgagcc cgccaaggcc
      601 aaggcgatcc gcgagaccgc tctgttctac acccgccagc ggaccaccga cctgctgacg
      661 caaatggcgg tgaccgtgca gggctaccta gcgctcgacc tggtcaagaa gaacaatgtc
      721 gagctggtca agggcgtcga ccgggcctcg accaccaccg tctcggcgct tcgcaccgcg
      781 gtcaccgtcg cccaggcgat gaccaaccag cggctggtgc tcgagcagat cggggcgctc
      841 aacacgacca ccgcggggat gatcgattcg acgggcgagc tgctgcgcac ccagactggg
      901 gcgatccacg agcaggccgc gtcctcgacg atccccctcg agacgctgca gcgggcgttc
      961 cagaacatct acgagacgat ggaccagatc gaccgcttca agctggaggc gctcggcaac
     1021 atgaagcaga cggtcgagac gctgtcgacg gaggtcgaga agtcgaaggg ctatatcgcc
     1081 cgcgccgaag gcgtcgagca gggcaagctg aaaggggccg cctcgccgtt cactcccatc
     1141 gagagcaaat gaacaccgac cgatacaacc aggtcatcgc ccgcgccgac cgcgtgatga
     1201 aggcgctcga cgagcgcgac gggccggtgc gcgccgccgc ccgccgcgag cgccagcggc
     1261 tcaacgccga cctcggccgc cggctcgccc gggtcggggt cggggtcggc ctggtcagca
     1321 tcgccaccat cgtcatcggg ctgatcgtgc cgatcgggat gttcggcttc ctcgccgcgg
     1381 tcgggctggc gatcgggatc gcggcgatgc tcgcggtgat tccgtcacag gcgcgcagcg
     1441 ccgccgctcc ggccgccgac ctgcccaacg gcgagatggt ccagcgcttc gattcctata
     1501 tctaccgaag ccgcgccgcc ctccccgcgc ccgcccagtc cgagctcgac gcgatcagcg
     1561 cggcgctgcc ctcgcttcgc cagacgctgg agcgggtcga tgcgctcgac cccaacgcgc
     1621 aggacgcccg gcggctgatg tcgctgcacc tgccgggcct gatcgaccgc taccgccagg
     1681 tcccgcacgc ctatcgcaac gagcgcgacg gcgaagggat cagcgtcgac gagcggctgg
     1741 tcgagggact cgccgccggc cgcgccgcgc tcgccgagat ttccgaaaag ctcgcccgct
     1801 ccgacatgat cgcgttcgag acccagggcc gcttcatcaa gtcgcgctac ggcgaggagc
     1861 gcatcgacca atagggccta gcccaccacc ccgtcggctt tgagccgctc gaattcctcc
     1921 ccgctcaccc ccagctcgcc cagcaccgcg cccgtatgct cgccgagcgc cggcggcggc
     1981 aggtcggaat cggcccgtgc cccgtcgatc cgaaccggcg agccgaccag cggcatttcg
     2041 ccgatcgtcc ggaccatcgc ccggtgctgc gcctggacat cggccagcgc ctggccgacc
     2101 cggttgatcg gcccggccgg gattcccgct gattcgagct tgtcgaacca ttccgcggcg
     2161 ggcttgcggg cgatgcactg agcgatcagc cggacgatct catcgcggtt ggcgacccgg
     2221 gcgccgttgg tcgcgaagcg ctcgtcgctt gcccattcgg gatggcagca cagctcggcc
     2281 agccgcgcga actggcggtc gttgccgatg gcgatgacga tcggccggtc ggccgcctcg
     2341 aatggctggt agggcacgat gttggggtgg gtgttgccct gccgcggcgg gtcctcgccc
     2401 gacaccaggg cgttcgacgc ctggttggcg agcatcgcca gctggacgtc gagcagggcc
     2461 atgtcgatgt gcgcgccctc gcccgtcttt tcgcgccggt gaagcgcggc gaggatcgcc
     2521 accgccgtgt agagcccggt gaacaggtcg gcgaccgcga ccccgacgcg catcggccca
     2581 ccgccgggct cgccgtcggg ccggccggtg acgctcatca atccgcccat gccctggatg
     2641 atgtaatcgt agccggcgcg gtccgcgtag ggcccgtcct ggccgaagcc ggtgatcgag
     2701 gcgacgatca gccggggatt ctcgcgccgc aacgattccg cgtcgagccc gaaccgcgcc
     2761 agggcgccga ccttgaagtt ctcgacgacg acgtcgcatc gcgcggccag ccggcggacc
     2821 agctccgcgc cctcgccggt ggcgaaatcg atcgctgccg agcgcttgcc gcggttggcg
     2881 gcgaggaagt aagcggcgac ccggccctcg ccctcgccgt gccacggcgg tccccagtgg
     2941 cgcgtgtcgt cgccggctcc gggccgctcg atcttgatca cgtcggcgcc gaggtcgccc
     3001 aacaattgcg tgcaccacgg cccggcgagc acccggctca aatcgagcac ccggatcccg
     3061 gccagcggag cggtcacgcc cgcttccatg gccggcggtc ggcgagcacg gcccgcgccg
     3121 cgttgtgccc gggcgctccg gtcaccccgc cgcccgggtg cgcgcccgac ccgcacaggt
     3181 acagcccctt caagggcatc cggtaatcgg cggcgccgat catcggccgg gcgctgaaca
     3241 attggtcgag ccccatcttg ccgtggaaga tgtcgccgcc gacgaggccg aagcggcgct
     3301 cgaggtcgag cggcgagtgg atctgccggc cgagcaacga ttgggcgaag ccgggggcgt
     3361 gcgaatcgac ggtggcgatg atcgcgtccg cggccgcctc gcgctcatgg tcccagctgc
     3421 gccccggccc gaggtcgaag cggaaatgct ggcagaacag cgacgcgacg tgcttgcccc
     3481 tgggcgccag gcccgggtcg agggtcgagg ggatcagcat ctcgatgatc ggcttctgcg
     3541 cccagccgtc gcggctcgca tcgacgaacg cgcggtc
//
LOCUS       DASTZO010000138         2437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_246925,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000138 DASTZO010000000
VERSION     DASTZO010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246925"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VFU20_03885"
     CDS             complement(<1..122)
                     /locus_tag="VFU20_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU5285639.1"
                     /translation="MSEDVLVSADAGIGRIRLNRPQAIHALTRAMCDTMSEALLG"
     gene            complement(149..1306)
                     /locus_tag="VFU20_03890"
     CDS             complement(149..1306)
                     /locus_tag="VFU20_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5285640.1"
                     /translation="MSQFDLTDDQLQIQDMARKFTADAITPFAAEWDEKHIFPRDTIR
                     EAAGLGFGAIYVSEESGGIGLGRLEAALIMEAMAYGCPSTSAFISIHNMAAWMIDRYG
                     SAEVKQKYLPSMIPMERIGSYCLTEPSSGSDAAALKTKAVLAKDSKLGDHYVVSGSKA
                     FISGGGENEIYVTMVRTGEEGPKGISCLVIDKDMPGVSFGGQEKKLGWHSQPTAQVNF
                     DEVRVPVANRVGAEGEGFRIAMAGLDGGRLNIGACSLGGGQRCLDEAIRYTKERKQFD
                     TPIADFQATQFTLADMETELQAARYLLYVAAAKVTANAPDKTRFAAMAKRLATDTGSS
                     VVDRALQLHGGYGYLMDYPIERFWRDLRVHSILEGTNQIMRVIVSRDLLRQ"
     gene            complement(1319..1945)
                     /gene="gstA"
                     /locus_tag="VFU20_03895"
     CDS             complement(1319..1945)
                     /gene="gstA"
                     /locus_tag="VFU20_03895"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF007831.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HEU5285641.1"
                     /translation="MMKLYYSPGACSQAVHIVLHETGFSHDSIRVDLKAKTIADGSDY
                     LRINPKGSVPALDLGNGEVLTENAVVLQYLGDRAGSSDLLPPLGDFRRYRVLEWTNFV
                     ATELHKGFGPLFHGAAGEESKQLARDLLGRKFEYVDRQLGEGPYLMGDRFTLADAYLF
                     VMLGWTRMHRIDLARWPNLVAFRERLNQRPSVRQVLEFEGLVEKEAAS"
     gene            complement(2010..2210)
                     /locus_tag="VFU20_03900"
     CDS             complement(2010..2210)
                     /locus_tag="VFU20_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285642.1"
                     /translation="MSAAARSGILLALALAACNGAPRDSETDNAAVTDVEALPPDESA
                     ATPTDQLANGATEPPNADNARY"
     gene            complement(2194..>2437)
                     /locus_tag="VFU20_03905"
     CDS             complement(2194..>2437)
                     /locus_tag="VFU20_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285643.1"
                     /translation="STRGDAKGLLLVSSDGLRFYESRATPVRNVESSADSIRADFAFT
                     GEGQTWTKFQTLALEDDRLVRTESSPMASFTYVRCR"
BASE COUNT          376 a          797 c          830 g          434 t
ORIGIN      
        1 ccgagcaggg cctcggacat ggtatcgcac attgcccgag tcagcgcatg gatcgcctgc
       61 ggccggttga gtcggatacg gccgatgccg gcgtcggcgg agaccagcac gtcctccgag
      121 atcaggacat cgtcgctccc gggacggttc attgccgaag caggtcccgg ctgacgatga
      181 cgcgcatgat ctggttggtg ccctcgagga tcgaatggac cctgaggtcg cgccagaagc
      241 gctcgatcgg ataatccatc agatagccgt agccgccgtg cagctgcagc gcccggtcga
      301 cgaccgacga gccggtgtcg gtcgcgagcc gcttggccat cgccgcgaag cgtgtcttgt
      361 cgggggcgtt ggcggtcacc ttggccgcgg cgacgtagag gaggtaccgc gccgcctgga
      421 gctcggtttc catgtcggcg agcgtgaact gggtcgcctg gaagtcggcg atcggagtgt
      481 cgaactgctt gcgctccttg gtgtagcgga tcgcctcgtc gaggcaccgc tggccgccgc
      541 ccagcgagca ggcgccgatg ttgagccggc cgccgtcgag cccggccatg gcgatccgga
      601 agccttcgcc ctcggctccg acgcggttgg cgaccgggac ccggacctcg tcgaaattga
      661 cctgcgcggt cggttgcgag tgccagccga gcttcttctc ctgcccgccg aagctgactc
      721 ccggcatgtc cttgtcgatc accaggcagg aaatgccctt cggcccctcc tcgccggtgc
      781 ggaccatggt cacgtagatc tcgttctcgc cgccgcccga gatgaaggcc ttggagcccg
      841 acaccacata atggtcgccc aatttggaat ccttggccag caccgccttg gtcttgagcg
      901 ccgcggcgtc cgagcccgac gacggctcgg tcaggcaata gctgccgatc cgctccatcg
      961 ggatcatcga cggaaggtat ttctgcttga cctcggcgga gccgtagcgg tcgatcatcc
     1021 aggcggccat gttgtggatc gagatgaacg cgcttgtcga cgggcagccg taggccatcg
     1081 cctccatgat cagcgccgcc tcgagccgcc cgaggccgat gccgcccgat tcctcgctga
     1141 cgtagattgc cccgaagccg agcccggccg cctcgcggat cgtgtcgcgc gggaagatgt
     1201 gcttctcgtc ccactcggcg gcgaacggag tgatcgcgtc ggcggtgaac ttgcgcgcca
     1261 tgtcctggat ctgcagctgg tcgtcggtga gatcgaactg ggacatggcc cagcgcctct
     1321 agcttgcggc ttccttctcg accagccctt cgaactcgag cacctggcgg accgacgggc
     1381 gctggttcag gcgctcgcgg aacgcgacga gattcggcca gcgagccagg tcgatgcggt
     1441 gcatccgggt ccagccgagc atcacgaaca gataggcatc ggcgagggtg aaccggtcgc
     1501 ccatcaggta cgggccctcg ccgagctggc gatccacata ttcgaatttc ctgccgagga
     1561 gatcgcgcgc gagctgcttg gactcctcgc cggccgcccc gtggaacaac gggccgaagc
     1621 ccttgtggag ctcggtcgcg acgaagttcg tccattcgag cacccggtag cggcggaagt
     1681 cgccgagcgg cggcagcagg tcggaggagc cggcgcgatc gcccagatac tggagaacga
     1741 ccgcgttctc ggtcagcacc tcgccgttgc cgaggtcgag cgccggcacc gagcccttgg
     1801 ggttgatgcg cagatagtcc gagccgtccg cgatggtctt cgccttgagg tcgacccgga
     1861 tgctgtcgtg gctgaacccg gtttcgtgaa ggacgatgtg caccgcctga gaacaggcgc
     1921 cgggtgaata atagagcttc atcatcgctc gttcctttcg tgtcttcacc cgccggcaag
     1981 ctgcgtaagg cagcgcggcc cgccctcgct cagtagcggg cgttgtccgc gttcggcggc
     2041 tcggtcgcgc cgttggccag ttggtcggtc ggggtcgcgg cgctctcgtc gggcggaagg
     2101 gcttcgacgt cggtcaccgc ggcgttgtcg gtttccgaat cgcggggcgc gccgttgcag
     2161 gcggccagtg ccagcgccag caggatcccc gatctagcgg cagcggacat aggtgaagct
     2221 cgccatcgga ctgctctccg tgcgcaccag cctatcatct tcgagcgcca atgtctggaa
     2281 cttggtccag gtctggcctt cgccggtgaa ggcgaaatcg gcgcggatcg aatcggcgga
     2341 gctttcgacg ttgcggaccg gggtcgcccg cgattcgtag aagcgcaggc cgtcgctgct
     2401 gaccagtagc agccccttgg cgtcgccgcg cgtcgac
//
LOCUS       DASTZO010000139        10123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_88855,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000139 DASTZO010000000
VERSION     DASTZO010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10123
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88855"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /gene="ppnK"
                     /locus_tag="VFU20_03910"
     CDS             <1..174
                     /gene="ppnK"
                     /locus_tag="VFU20_03910"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402837.1"
                     /note="catalyzes the phosphorylation of NAD to NADP;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HEU5285644.1"
                     /translation="LRVLDPTKRPVSAVADQTEVRDVAHVEIRVDRSTALTLLFDPEH
                     ALDERIAMEQFLT"
     gene            231..305
                     /locus_tag="VFU20_03915"
     tRNA            231..305
                     /locus_tag="VFU20_03915"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:263..265,aa:Asn,seq:gtt)
     gene            479..979
                     /locus_tag="VFU20_03920"
     CDS             479..979
                     /locus_tag="VFU20_03920"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HEU5285645.1"
                     /translation="MPTAVLDKEIVEAIASGPPVALHRGESDLPFVPYQEGVTFQLIQ
                     VDIEAGFFVVRVRGEPGRTIQRHKHTGEIFAFTLSGSWRYLEYPEINTAGSFLYEPAG
                     SVHTLHFPDSNTETTDVWFVLHGANLNLDDEGNVESVLDAGVALEVYRAGCRDAGLPE
                     PDVIGA"
     gene            complement(1113..4274)
                     /gene="putA"
                     /locus_tag="VFU20_03925"
     CDS             complement(1113..4274)
                     /gene="putA"
                     /locus_tag="VFU20_03925"
                     /EC_number="1.2.1.88"
                     /EC_number="1.5.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010233934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010133 - proline catabolic process to
                     glutamate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase PutA"
                     /protein_id="HEU5285646.1"
                     /translation="MPRSAGLKTAPSPAARPVDRTELRRAYRPDEEQVALERLSEAQL
                     EPAVRSEATATARALVKGIRAHKPSGLDAFLHTYDLGSDEGIALMCLAEALLRIPDAR
                     TADELIADKLAGPDWAEKAGQSNSAFVNAATFSLLLTGKVLEGANDRSDNWRAVLGRT
                     VGRLGEPVVRTAVTQAMRILGRQFVFGRTIDEALRRAEPDRAAGLTHSFDMLGEAART
                     HEDAARYAKAYAEALDRIGAEARGGIVLSPGISVKLSALHPRYEFTHADEAKAAMLPV
                     LRDLAMRASTADVHFTIDAEEADRLELSLDLFEALLADDSLFANGWAGFGLAVQAYQK
                     RAAPLCDWVAEAARAHRRKLMVRLVKGAYWDTEIKAAQVAGLPDYPVFTRKVATDVSF
                     LACAKKLLAASDAIYPAFATHNANSIGAIKAIAGKAPFEFQRLHGMGEALYEELGKLE
                     AAIGDPPTPVRIYAPVGSHKELLAYLVRRLLENGANSSFVNRIADEQVSLNELVRDPV
                     AELAECKPRRNPAMPLPRMIFGEARRNSAGVDLSDPLVRGPLLERLKALGARTWKAAP
                     TGGGKGQGRVVTSPHDRRIKVGTVLEASARDVDRMVRAAAAAQPGWDALGGEARARLL
                     DRAADLFEEHRDDLFSLCIREGGKTLIDAVLEVREAVDFLRFYASEARRQFSRPLPLP
                     GPTGEQNELRLHGRGVFASISPWNFPLAIFTGLVSAPLAAGNAVVAKPAGQTPLIGAL
                     AVELMHKAGIPRDVVQLAPGEGRVVGGALTAHPLLAGVVFTGSTETAKMINRTLAERD
                     GPIIPFIAETGGQNAMIVDSSALPEQVTRDVISSAFQSTGQRCSALRVLFVQEEVADG
                     IIEMIAGAMRALSVGDPSDLAADVGPVIDEDAKRQLDEHIKWLDKNARKICRLEPPAE
                     AANGCFVPPAFYEIKSLSQLNRENFGPILHVVRFAAEDLGKVVDDINATGYGLTLGLQ
                     SRIDTVREFVEQRARVGNFYVNRNQIGAVVESQPFGGEGLSGTGPKAGGPHYVARFAT
                     ERVTCIDTTAAGGNATLMAALEG"
     gene            complement(4253..6979)
                     /gene="secA"
                     /locus_tag="VFU20_03930"
     CDS             complement(4253..6979)
                     /gene="secA"
                     /locus_tag="VFU20_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU5285647.1"
                     /translation="MFAALAKSIFGSANDRYVRSLGKYVDAINALEPAISALSDDQLR
                     AQTDLFRQRLAGGETMDQLLPEAFATVREAATRTLGQRHYDVQLIGGIALHRGEIAEM
                     KTGEGKTLVATLAVYLNALPEKGVHVVTVNDYLARRDADWMGQIYRFLGLSVGVIVPN
                     LSDQQRRDAYNCDITYATNNELGFDYLRDNMKYSREQMVQRPFNFAIVDEVDSILIDE
                     ARTPLIISGPTDDKSELYMSVDALVKTLVPEDYELDEKLKSVVLTEDGTEKAERMLED
                     AGIIEGRNLYAIANTQVVHHLNQALKANVMFKKDIDYIVKDGKIVIIDEFTGRMMEGR
                     RWSDGLHQAVEAKEGVQIEPENQTLASITFQNYFRMYPKLSGMTGTALTEAPEFFDIY
                     RMNVVSIPTNVPVQRTDEDDEFYKNITDKFAAIATEIKARQEKGQPVLVGTVSIEKSE
                     MLSEYLTREGITHEVLNARFHEKEAHIVAQAGALGAVTIATNMAGRGTDIQLGGNVDF
                     RIEDELADLPEGRERDQAIERIKAEVAEQKRLVIEAGGLFVLGTERHESRRIDNQLRG
                     RSGRQGDPGLSRFYLSLDDDLLRIFGPQTMFARLMNKNLEDGEAIVSPWISKAIETAQ
                     KKVEARNYDIRKQVVEYDDVMNDQRKVIYEQRADIMDAESVGDVIAEMRAETAESIVT
                     AHCPPGTYPEQWDVEGLKESVDAIFGLQPPIDAWLEEEAVEQEIITERLQKLADEVME
                     EKVREVDPQRWAEAEKNILLQTLDHFWKEHLATLDALRAVIHLRSYAQKKPIDEYKQE
                     AFLLFERLLIAIREDVTRVLMRAQVQLEAPPPPVLPEFITQHLDPFSGDDDTADVDLA
                     TGAILSNLPPLNIPRPQMPAAEDELAQAPVSRNAPCPCGSGRKYKHCHGQLA"
     gene            7109..7555
                     /locus_tag="VFU20_03935"
     CDS             7109..7555
                     /locus_tag="VFU20_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU5285648.1"
                     /translation="MAELTGRCLCGAVRYRLDGRPFDCGWCHCRTCQLASGAPAMAFA
                     SVRTGEWIVTQGEEHCRRIRSSSIAERLFCGRCGTPLAIDYDIQPETFDFSIVTLDDP
                     DAIRPAFHIFWSSKVAWFDPGDDLPRYDRFRPDTVGLAGTDPPDDA"
     gene            complement(7552..7872)
                     /gene="trxA"
                     /locus_tag="VFU20_03940"
     CDS             complement(7552..7872)
                     /gene="trxA"
                     /locus_tag="VFU20_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5285649.1"
                     /translation="MATKSVTDQSFATDVLGAPGPVLVDFWAEWCGPCRMIAPALEEI
                     STELGDKVTIAKLNIDENPDVPGRYGVRGIPTMLLFKGGQPVAQKVGAAPRSQIQQWL
                     EAQL"
     gene            complement(7929..>10123)
                     /locus_tag="VFU20_03945"
     CDS             complement(7929..>10123)
                     /locus_tag="VFU20_03945"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEU5285650.1"
                     /translation="WEIIKPLAAEYFSGSSESEGRVRTIFMVGDFKQAIFGFQGTDPN
                     EFQRARNWVRQHSSALREAEDDADDPRQLALEFRDLSIDASFRSAPAILDVVDAVIDE
                     VGYRAMGLPDEPNRHRAFFDRRPGLVELWKPFSVESDGDSEEAEEGWLDERDRKYATA
                     LAGQIKRWLDEAPVLDSTKRPLSPGDVLVLVRSRGELASLIVARLFAANVPVAGIDRL
                     HLSKPFAVRDLLAAVAFAVQPLDDLNLANLLVSPLVGWSQDQLFDLAYGRDKVPLWRL
                     LRERAAEQPHLAEAHDALGSLLAMADYTTPARFLETILSGPLEGRRKLYRRLGLASRD
                     PIDELLASAMEFERSETPSLDRFLAWFGRGDVEVQRDPSAPAQAVRVMTVHGAKGLEA
                     PFVILADSTADPARIGGTARTISFPLDAEQTIKVPLVRPRKAERVSPFAELMASKEES
                     DLEEHWRLLYVGLTRAIERLVVAGVKPKGERAENCWHTRVERALIALGAQPERDAHWG
                     EAIRHRGNVTAAPPRPKKPRIDIQSPPVPEWARRPAPAENRPPRPLAPSALIEDREAA
                     ALPSAEQRAAQRRGTLVHQLFERLAGVEPGQRPSAAEHWLERSAGVSEPDERRAIADM
                     VCSIIGDTSLAPLFGPGSLAEAPIAATLPDGLVVAGTVDRLLVEDDRVSVIDFKTGRV
                     PPSDAQIPAAHRAQMAAYAEALGVIFPGRSIRSALLYTAGARLFELDG"
BASE COUNT         1516 a         3418 c         3440 g         1749 t
ORIGIN      
        1 cttcgggtcc tcgatccgac caagcgcccg gtctcggcgg tggcggacca gaccgaagtg
       61 cgcgacgttg cgcacgtcga gatccgcgtg gaccgttcga ccgccctcac cctgctgttc
      121 gatcccgagc atgcgctcga cgagcggatc gcgatggagc aattcctcac ctgaagcctt
      181 gtctttggag cgggcttcgc tatctagccg ccacgcttcg acagaggtgt tccccggtag
      241 ctcagcggta gagcgttcga ctgttaatcg aatggtcgcc tgttcgaatc aggcccgggg
      301 agccatcctt tccaccattt agcgcggaaa gccgcccaac cgagccggtt gggattgccc
      361 attggggatg ggcttgcggt catggtagga ggaggccacc gcgccctgtt tcgaatcctg
      421 gcccttgcgc ggctgagaga cctggattct cccggcttga ggtaaggagc acgtcccgat
      481 gcccacggct gtcctggata aagaaatcgt cgaagctatc gccagcgggc cgccggtcgc
      541 gcttcaccgc ggtgagagcg acctgccgtt cgtgccgtat caggagggtg tcaccttcca
      601 gctcatccag gtcgacatcg aagcgggctt ctttgtggtt cgggtgcgcg gcgagcccgg
      661 aaggaccatt cagcgccaca agcataccgg cgagatcttc gcgttcacct tgtccgggtc
      721 gtggcgctat ctcgaatatc cggagatcaa taccgccgga tcgtttctct atgagccggc
      781 cggctcggtc cacacgctgc actttccgga ttcgaacacc gagaccaccg acgtctggtt
      841 cgtactgcac ggcgccaatc tcaacctcga tgacgagggc aatgtcgaat ccgttctcga
      901 tgcgggcgtg gcgctcgagg tctatcgcgc cggttgccgc gacgcggggt tgccggagcc
      961 ggatgtcatt ggcgcgtgaa acgaaacgga gcggccggcg cgtgcgtccg accgctccgg
     1021 cgggacgcgt tcatgtcccg tgaaggaccg ccccgcaggc catcaacgat tgcgtttcgg
     1081 ccccgctcca gagcgtcccg agaagggaca atctagcctt cgagagcggc catcagggtc
     1141 gcgttgccgc ccgccgcggt ggtgtcgatg caggtcaccc gctcggtcgc gaagcgcgcg
     1201 acataatgcg ggccgccggc cttgggcccg gtgccgctca acccctcgcc gccgaacggc
     1261 tggctttcga ccaccgcgcc gatctggttg cggttgacgt agaaattgcc gacccgcgcg
     1321 cgctgctcga cgaactcgcg caccgtgtcg atgcggctct gcaagccgag cgtcaggccg
     1381 tagccggtcg cgttgatatc gtcgacgacc ttcccgagat cctcggcggc gaaccggaca
     1441 acatgcagga tcgggccgaa attctcgcga ttcaattggc ttagcgactt tatttcatag
     1501 aatgcgggag gaacgaagca gccgttggcg gcctcggcgg gcggctccag ccggcaaatc
     1561 ttccgggcgt tcttgtcgag ccacttgata tgttcgtcga gctgccgctt ggcgtcttcg
     1621 tcgatcaccg gcccgacgtc ggccgcaaga tcgctggggt cgccgacgga cagcgcgcgc
     1681 atcgccccgg caatcatctc gatgatgcca tccgcgacct cctcctggac gaacagcacc
     1741 cgcaatgccg agcagcgctg gccggtgctt tggaaggcgg acgagatgac gtcgcgggtc
     1801 acctgctcgg gcagggccga gctgtcgacg atcatcgcgt tctggccgcc ggtctcggcg
     1861 atgaacggga tgatcggccc gtcgcgctcc gccagcgtgc ggttgatcat tttcgcggtc
     1921 tcggtcgaac cggtaaatac gaccccggca agcagcggat gggcggtcag cgccccgcca
     1981 acgacccggc cctcgccggg tgcgagctgc acgacgtccc tggggatgcc ggctttgtgc
     2041 atcagctcga ccgcgagcgc cccgatcagc ggcgtttgtc cggccggctt ggcgacgacc
     2101 gcattgcccg ccgccagcgg cgccgacacc agaccggtga agatcgccag cggaaaattc
     2161 catggggaaa tgctggcgaa cacgccccgc ccgtgcaggc ggagctcgtt ctgctcgccc
     2221 gtcggccccg gaagcggcag cggccgggag aactggcgcc gcgcctccga cgcgtagaag
     2281 cgcaggaaat cgaccgcctc gcgcacttcg agcaccgcgt cgatcaaggt cttcccgccc
     2341 tcgcggatgc acagcgaaaa caggtcgtcg cgatgctcct cgaacaggtc ggcggcgcga
     2401 tcgagcagcc tggcgcgcgc ctcgccgccc agcgcgtccc acccgggctg cgcggccgcg
     2461 gcggcgcgga ccatgcggtc gacgtccctg gcgctcgcct cgagcaccgt gccgaccttg
     2521 atccggcggt cgtgcggcga ggtcacgacc ctgccctgcc ccttgccgcc accggtcgga
     2581 gcggccttcc aggttcttgc tcccagggcc ttcaggcgct cgagcagcgg cccgcgaacc
     2641 agcggatcgg acaaatcgac tccggcgctg ttgcggcgcg cttcaccgaa gatcatgcgc
     2701 ggcagcggca tcgccgggtt gcgcctgggc ttgcactcgg cgagctcggc gacgggatcg
     2761 cggaccagct cgttgagcga cacctgctcg tcggcgatcc ggttgacgaa cgaggaattg
     2821 gcgccgttct cgagcagccg gcggacgagg taggcgagca gctccttgtg gctcccgacc
     2881 ggtgcgtaga ttcgcaccgg cgtcggcgga tcgccgatcg cggcctcgag cttgccgagc
     2941 tcctcgtaga gcgcttcgcc catgccgtgc aggcgttgga actcgaacgg ggccttgccg
     3001 gcaatcgcct tgatcgcgcc gatgctgttg gcgttgtggg tcgcgaacgc cgggtagatg
     3061 gcgtcgctgg cggccagcag cttcttcgcg caggcgagga acgagacgtc ggtcgccacc
     3121 ttgcgcgtga acaccggata atcgggcagt ccggcgacct gggcggcctt gatctcggtg
     3181 tcccaatagg cgcccttgac cagccggacc atcagcttgc gccgatgcgc ccgggccgcc
     3241 tcggcgaccc agtcgcacag cggcgccgcg cgcttctgat aggcctggac ggcgagcccg
     3301 aatccggccc agccatttgc gaatagcgaa tcgtcggcga gcagcgcctc gaacaggtcg
     3361 agcgacagct cgagccggtc ggcttcctcg gcgtcgatgg tgaaatggac gtcggcggtg
     3421 ctcgcccgca tcgccaggtc gcggagcacc ggcagcatcg ccgctttggc ctcgtcggcg
     3481 tgggtgaact cgtagcgcgg gtgaagcgcc gacagcttca ccgaaatgcc gggcgacagg
     3541 acgatgccgc ccctggcttc cgcgccgatg cggtcgagcg cctcggcata ggccttggca
     3601 tagcgcgcgg cgtcctcatg cgtgcgcgcc gcctcgccga gcatgtcgaa gctgtgggtc
     3661 agccccgccg ctcggtcggg ctcggcacgg cgcagcgcct cgtcgatggt gcggccgaac
     3721 acgaactggc gtccgaggat gcgcatcgcc tgggtgaccg cggtccggac caccggctcg
     3781 cccagccgcc cgacggtccg gccgagcacc gcgcgccaat tgtcgctccg gtcgttggcg
     3841 ccctcgagca ccttgccggt cagcagcagc gagaaagtgg cggcgttgac gaacgcggag
     3901 ttcgactgtc cggccttctc cgcccagtcc ggccccgcga gcttgtcggc gatcagctcg
     3961 tcggcggtgc gggcgtcggg aatccgcagc agcgcctcgg ccaggcacat cagggcgatg
     4021 ccctcgtcgc tgccgaggtc gtaggtgtgg aggaacgcgt cgagcccgga cggcttgtgc
     4081 gcccttatgc ccttgaccag cgcgcgcgcg gtcgcggttg cttcgctccg gacggctggc
     4141 tcgagctgcg cctcggacag gcgctcgagc gcgacctgct cctcgtcggg gcggtaggcg
     4201 cggcgaagct cggtgcggtc gaccggccgt gccgccggcg atggggcggt cttcaggcca
     4261 gctgaccgtg gcaatgcttg tatttccggc ccgagccgca cgggcacggg gcgttgcggc
     4321 tgaccggcgc ctgggcgagc tcatcctcgg cggcgggcat ctgcggccgc ggaatgttga
     4381 gcggcggcag gttggacagg atcgccccgg tcgcgagatc gacgtccgcg gtgtcgtcgt
     4441 cgccggagaa cgggtcgagg tgctgggtga tgaactcggg cagcaccggc ggaggcgggg
     4501 cctcgagctg gacctgcgcc cgcatcagca ccctcgtcac gtcctcgcgg atggcgatca
     4561 gcagccgctc gaacagcagg aacgcctcct gcttatattc gtcgatcggc ttcttctggg
     4621 cgtagctgcg aaggtggatg accgcccgga gcgcgtcgag ggtcgccaga tgctccttcc
     4681 agaaatggtc gagcgtctgc agcaatatgt tcttctcggc ctcggcccag cgctgcggat
     4741 cgacctcccg gaccttctcc tccatcacct cgtcggcgag cttctgcagc cgctcggtga
     4801 tgatctcctg ctcgaccgct tcctcctcga gccaggcatc gatcggcggc tgcaggccga
     4861 agatcgcgtc gacgctctcc ttcaaaccct cgacgtccca ttgctcgggg taggtgccgg
     4921 gcgggcagtg agcggtgacg atcgactccg cggtctcggc gcgcatctcg gcgatgacgt
     4981 cgccgacgct ctcggcatcc atgatgtcgg cgcgctgctc gtagatcacc ttgcgctggt
     5041 cgttcatgac gtcgtcatat tcgacgacct gcttgcggat gtcgtagttg cgcgcctcga
     5101 ccttcttctg agcggtctcg atcgccttgg agatccacgg gctgacgatc gcctcgccgt
     5161 cctcgaggtt cttgttcatc agccgcgcga acatggtctg cggcccgaag atgcgcagca
     5221 agtcgtcgtc gaggctcaaa tagaagcgcg acaggccggg gtcgccctgg cggcccgatc
     5281 ggccgcgcag ctggttgtcg atgcgccggc tctcgtgccg ctcggtgccg agcacgaaga
     5341 gcccgccggc ctcgatcacc agccgcttct gctcggcgac ctcggccttg atgcgctcga
     5401 tcgcctggtc gcgctccctg ccctcgggca ggtcggcaag ctcgtcctcg atccggaaat
     5461 cgacgttgcc gcccagctgg atgtccgtgc cgcgcccggc catgttggtg gcgatcgtca
     5521 ccgcgccgag cgctccagcc tgggcgacga tatgcgcttc cttctcgtgg aagcgggcat
     5581 tcagaacctc gtgcgtgatg ccttcgcggg tcagatattc ggacagcatc tccgatttct
     5641 cgatcgacac ggtgccgacg aggaccggct ggcccttttc ctgccgagcc ttgatctcgg
     5701 tggcgatggc ggcgaacttg tcggtgatat tcttgtagaa ctcgtcgtcc tcgtcggtgc
     5761 gctggaccgg cacgttggtc gggatcgaga cgacgttcat ccggtagatg tcgaagaatt
     5821 ccggcgcctc ggtcagagcg gtgccggtca tgcccgacag cttcgggtac atccggaagt
     5881 aattctggaa ggtgatcgag gcgagcgtct ggttctcggg ctcgatctgc acgccttcct
     5941 tggcctcgac cgcctggtga agcccgtcgg accagcgccg cccctccatc atccggccgg
     6001 tgaactcgtc gatgatgacg atcttcccgt ccttgacgat atagtcgatg tccttcttga
     6061 acatgacgtt ggccttcaac gcctggttca ggtgatggac gacctgggtg ttggcgatcg
     6121 catagaggtt gcggccctcg atgatgccgg cgtcctcgag catccgctcg gccttttcgg
     6181 tgccgtcctc ggtcaggacg acgctcttca atttctcgtc gagctcataa tcttccggca
     6241 ccagcgtctt caccagcgcg tccaccgaca tgtagagctc ggacttgtcg tcggtcgggc
     6301 cggagatgat cagcggggtc cgcgcctcgt cgatcaggat cgagtccacc tcgtcgacga
     6361 tggcgaagtt gaacggccgc tggaccatct gctcgcgcga gtatttcatg ttgtcgcgca
     6421 aatagtcgaa gccgagctcg ttgttggtcg cgtaggtgat gtcgcaattg taggcgtcgc
     6481 gccgctgctg gtcgctgagg ttggggacga tgaccccgac cgatagtccg agaaagcggt
     6541 agatctgccc catccagtcg gcgtcgcggc gggcgagata gtcgttgacg gtcaccacgt
     6601 ggacgccctt ctcgggcagc gcgttcaggt agaccgcgag cgtcgcgacc agcgtcttgc
     6661 cctcgccggt cttcatctcg gcgatctcgc cgcggtggag cgcgattccg ccgatcagct
     6721 ggacgtcgta gtggcgctgg ccgagcgtcc gagtcgccgc ctcgcggacc gtcgcgaagg
     6781 cttcgggcag caattggtcc atggtctcgc cgcccgccag gcgctggcgg aacaggtcgg
     6841 tctgggcgcg cagctggtcg tcgctcagcg ccgaaatggc cggctcgagc gcgttgatcg
     6901 cgtcgacata tttgccgagg ctccgcacat aacggtcgtt ggccgatccg aagatactct
     6961 ttgccagtgc ggcgaacatg acaatccctt aaatggcggg acgcgcgcca cgggcgatcc
     7021 gccagcgggc gatccgcctc gcccgcgcgc gggcaactgc ggggcgcgat gtaggcagca
     7081 cccccgtctt agtcaacgaa ggaccccgat ggccgagctc accggacgat gcctgtgcgg
     7141 agcggtccgc taccggctcg atggccggcc gttcgattgc ggctggtgcc attgccggac
     7201 ctgccagctc gccagcggag cgccggcgat ggccttcgcc agcgtccgga ccggcgaatg
     7261 gatcgtcacc cagggcgagg agcattgccg gcggatccgt tcgagcagca ttgccgagcg
     7321 actgttctgc ggccgctgcg gcactccgct ggcgatcgac tacgacatcc agcccgagac
     7381 gttcgacttc agcatcgtca cgctcgacga tccggacgcg atccggcccg cgttccacat
     7441 cttctggtcg agcaaggtcg cctggttcga ccccggcgac gatctgcccc gatacgaccg
     7501 cttccggccc gacacggtag ggctcgcggg aacggatccg ccggacgacg cctagagctg
     7561 cgcctccagc cactgctgga tctggctgcg cggcgcggcg ccgaccttct gcgcgaccgg
     7621 ctggccgccc ttgaacagaa gcatggtcgg gatcccgcgc accccgtagc ggccggggac
     7681 atcggggttc tcgtcgatgt tgagcttggc gatcgtcacc ttgtcgccga gctcggtcga
     7741 aatttcctcg agcgcggggg cgatcatccg gcacggcccg caccattcgg cccagaaatc
     7801 gacgagcacc gggcccggag cgcccagcac atcggttgca aagctctgat cggtgacgga
     7861 tttggtggcc atggcgggcg actctccttt tgaggctgcg ggccatatgg ggccttggcc
     7921 gtcgcctctc aaccgtcgag ttcgaacagt cgcgctccgg cggtgtagag cagcgccgag
     7981 cggatggagc gacccgggaa gatcaccccg agagcctcgg cataggcggc catctgggcg
     8041 cgatgggcgg ccggtatctg cgcgtccgaa ggcggcaccc gcccggtctt gaagtcgatc
     8101 accgagaccc ggtcgtcctc gaccagcagc cggtcgacgg tcccggcgac gactaggccg
     8161 tcgggaaggg tcgcggcgat cggcgcttcg gcgagcgagc ccgggccgaa cagaggcgca
     8221 aggctcgtat cgccgatgat cgagcagacc atgtcggcga tggcccggcg ctcgtcgggt
     8281 tccgacacgc ccgccgagcg ctcgagccag tgctcggccg ccgacgggcg ctgcccgggt
     8341 tcgacaccgg ccaaccgctc gaacaattgg tggaccagcg ttccgcgccg ctgagccgcg
     8401 cgttgctcgg cgcttggcag ggcggccgcc tcgcggtcct cgataagcgc cgagggagcc
     8461 agcggccgcg gcggccggtt ctccgccgga gcaggacggc gcgcccactc ggggaccggc
     8521 ggagattgaa tgtcgatgcg tggcttcttc ggcctcggcg gagcggccgt gacgtttccg
     8581 cgatggcgga tcgcctcgcc ccagtgcgcg tcccgctccg gctgcgcgcc gagcgcgatc
     8641 agcgcccgct cgacccgggt gtgccagcaa ttctcggccc gctcgccctt cggcttgacg
     8701 ccggcgacca ccagccgctc gatcgcccgg gtcaggccga cgtagagcag gcgccaatgc
     8761 tcctcgaggt cggattcctc cttgctcgcc atcagttcgg cgaacggaga tacgcgctcg
     8821 gccttgcgcg gccggaccag cggcaccttg atggtctgct ccgcatccag cgggaaactg
     8881 atcgttcggg cggtgccgcc gatcctggcc gggtcggcgg tcgaatcggc gaggatcacg
     8941 aacggcgctt caaggccctt tgcgccgtgc acggtcatta cccgcaccgc ctgggccggc
     9001 gccgacggat cgcgctggac ctcgacatcg ccgcggccga accaggctag gaagcggtcg
     9061 agcgacggcg tttcgctgcg ctcgaactcc atcgcgctcg ccagcaattc gtcgatcggg
     9121 tcgcgcgacg cgagcccgag ccggcggtac agtttgcgcc ggccctcgag cggcccggac
     9181 aggatcgtct cgaggaaccg agccggggtc gtgtaatcgg ccatcgccag aagcgagccg
     9241 agcgcgtcgt gcgcctcggc caggtgcggc tgctcggccg cgcgctcgcg aagcagccgc
     9301 cacagcggga ccttgtcgcg gccgtaggca aggtcgaaca attggtcctg gctccagccg
     9361 accagcggcg agaccagcag gttggcgagg ttgaggtcgt cgagcggctg cacggcgaag
     9421 gcgaccgcgg cgagcaggtc gcggaccgcg aacggcttcg agagatggag ccggtcgatg
     9481 cccgcgaccg ggacgttggc cgcgaacagc cgggcgacga tcagcgacgc cagttcgccg
     9541 cggctgcgga ccagcaccag cacgtcgccg gggctcagcg gccgcttggt cgagtcgagg
     9601 accggcgcct catcgagcca gcgcttgatc tggccggcca gggcggtcgc atatttgcgg
     9661 tcgcgctcgt cgagccagcc ctcctcggct tcctcggagt cgccgtcgga ctcgaccgaa
     9721 aaaggcttcc acagctcgac cagcccgggc cgccggtcga agaaggcgcg atggcggttg
     9781 ggctcgtcag gcaggcccat cgcccgatag ccgacctcgt cgatcaccgc gtcgacgacg
     9841 tcgaggatcg ccggcgccga gcggaagctg gcatcgatcg acaggtcgcg gaactcgagc
     9901 gcgagctgcc gggggtcgtc ggcgtcgtcc tcggcttcgc gcagcgcgct ggaatgctgc
     9961 cggacccaat tccgggcgcg ctggaattcg ttgggatccg tgccctggaa gccgaagatc
    10021 gcctgcttga agtcgccgac catgaagatg gtgcgcaccc ggccttcgga ttcgctcgac
    10081 ccgctgaaat attccgcggc cagcggcttg atgatttccc act
//
LOCUS       DASTZO010000140         7728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_663533,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000140 DASTZO010000000
VERSION     DASTZO010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7728
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_663533"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VFU20_03950"
     CDS             <1..1443
                     /locus_tag="VFU20_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285651.1"
                     /translation="AERLERFDQALRSLQGVVEQEPLSDTQRLTVFRFGDIGDIETLA
                     RREGVAGFYIPRAAGPVAFVPAKNAIGKTSLYRDPEIDAQTVFFHEYAHHFMYRHFTA
                     AYPSWYREAFAEVNSTIELQDDGSFKLGSPPQSRASALLGGLNYSIKRMLLTSNRPNF
                     EDVYARYTYGWLLTHYLTFGGTRTGQLQKYLRAINAGTDAATAARQAFGDLDQLESEV
                     IRYKGRNRYPGAIVRPGSLPKPNVAMRKLGRDEEAIMRVRARSARGVDKKTSKDVAAD
                     ARAVAAKYTGSLAVQVALAEAEFDAERFDEAERAADAALAIDPRSGKALLYKGLVYLK
                     RSKADPRHYATARSWFGKASRVDPGDPSPLYNYYLTYKQAGAPPPQLAVQGLERAFEI
                     APYDAGLRVLTARQLLIDRKGALARQVLLPVAHSPHESKYSKALNEVAELIEANKLGE
                     ALAKLNARLDKDEKDDDDDVRAPSPVVEAL"
     gene            complement(1440..2489)
                     /locus_tag="VFU20_03955"
     CDS             complement(1440..2489)
                     /locus_tag="VFU20_03955"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013040858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU5285652.1"
                     /translation="MKTIDANQAWAAFERRDRALDGRLIGAVRTTGIYCKPSCPARRP
                     RREHVEFFADPQAARAAGYRPCLRCKPDEVGRDRAAVARAVRLIETAEEAPSLAELAA
                     AVGYALHHFQRLFTRDVGVSPAQYARALRARRAEAELTEDKSVTEAIYDSGYSAPSRF
                     YADAKERLGMTPSAWRDGGRGETIRWTVVASALGPLLVAATARGICRLTFEDDERSLM
                     RVFPNATLVEDDGSMGEWVAGALCAVERPAAAPDLPIDVAGTAFQEAVWRELRKIPPG
                     ETRSYAQIAAAVGKPGAVRATGSANGANPVAVLIPCHRVVRSDGSLGGYAGGIERKRR
                     LLEAERAAGQRSLDL"
     gene            complement(2555..3439)
                     /locus_tag="VFU20_03960"
     CDS             complement(2555..3439)
                     /locus_tag="VFU20_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285653.1"
                     /translation="MRILMLISAAAAAFAAHAPAAAKPASSQQFKAGTAISLDPAKAY
                     LLVRSPLNLDVKLLREARPDEVEAYKAERSAALEKARSKYARKLAGYERDIKAWNAMS
                     ANARAGQVKPERPVEPTDDNLAFKAIESDKFVTLWGGRVFDKPSQARLIAVDPGTYRL
                     YGQMIEGNNGSMLGFCLCMGSVDFDAAPGRITDLGSFRYPLAEALRDKVKPSWNGLTP
                     GKGGLTGMRVEPMRPADPVPASLASLPREAAAYRAAGKIDNFFGVQIDRLTALPGVLS
                     YRRDEVVDERSGGAAAAN"
     gene            complement(3439..3948)
                     /locus_tag="VFU20_03965"
     CDS             complement(3439..3948)
                     /locus_tag="VFU20_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285654.1"
                     /translation="MADRNLAPIEVDPAKVPLPELGFTEAAGDRDGYDKYFYFVRSET
                     DFATAYNDIRECDSLTRGIRTYLNGGTVPYPYAGTMAGALGGAIGSALADAIFGSALR
                     RNMRRTNMRTCMGYKGYSRHGLPKELWTKFNFEEGLSGVAEDERQKLLQMQAKAAIAG
                     KPQTEELEF"
     gene            4238..4795
                     /locus_tag="VFU20_03970"
     CDS             4238..4795
                     /locus_tag="VFU20_03970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEU5285655.1"
                     /translation="METSGGIQASLAGRYASALFGLARDERKIDAVGRSLDALDHALA
                     DSREFAALVASPLVGRDDAAKAFAALGPELGLDPLTVNFLGVLARNGRKRELKAIIRA
                     YRRIAADHRGEISAEVVSAHPLNDDQVEALKAQLRKRAGGADVTLDSRVDPEILGGIV
                     IKLGSEMIDASIRTKLNRLASAMKG"
     gene            4799..6328
                     /gene="atpA"
                     /locus_tag="VFU20_03975"
     CDS             4799..6328
                     /gene="atpA"
                     /locus_tag="VFU20_03975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU5285656.1"
                     /translation="MDIRAAEISRVIRDQIANFASDSEVSEVGTALSVGDGIARIHGL
                     DNVQAGEMVEFENGVKGMALNLEADNVGVVVFGSDAEIKEGSTVKRTGTIVDVPIGKG
                     LLGRVVDALGNPIDGKGPIEATERRRVEVKAPGIIPRKSVHEPVQTGLKALDALVPIG
                     RGQRELIIGDRQTGKTAVALDTFINQKAINASGKESEKLYCIYVAIGQKRSTVAQIVR
                     ALEENGAMDYSIVVAATASEPAPLQFLAPYTGCAMGEYFRDNGMHAVIVYDDLSKQAV
                     AYRQMSLLLRRPPGREAYPGDVFYLHSRLLERAAKMNEDNGAGSLTALPIIETQAGDV
                     SAYIPTNVISITDGQIFLETDLFFQGIRPAINVGLSVSRVGSAAQTKAMKKVSGSIKL
                     ELAQYREMAAFAQFGSDLDASTQRLLARGARLTELLKQPQFSPMPVEEQVASIFAGTQ
                     GFLDSVEVRDVTRYEAALLSYLRAEHADVLKTIRDSKALDDDTAKKLKDALTKFGKQF
                     A"
     gene            6335..7219
                     /locus_tag="VFU20_03980"
     CDS             6335..7219
                     /locus_tag="VFU20_03980"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEU5285657.1"
                     /translation="MASLKALKIRIGSVKSTQKITKAMKMVAAAKLRRAQQGAEAGRP
                     YATRMADVVASIASGVTVSPQSPKLLAGTGKDQTHLIVLATGDRGLAGAFNSNIVRAA
                     RRRADELIEDGKTVLFYLVGRKGRPVIQRLYPKAIIAQHDTTAMKAPGYADAQAIAAD
                     IIDRFITDGIDVVHLAYAQFKSTLVQEPVVQQIIPVPRAANDTRPASASNVAVEYEPD
                     EEAILEALLPRNIAVQIYRALLENQAGFYGSQMTAMDNATRNAGDMINRLSIQYNRQR
                     QAAITTELVEIISGAEAL"
     gene            7248..>7728
                     /locus_tag="VFU20_03985"
     CDS             7248..>7728
                     /locus_tag="VFU20_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU5285658.1"
                     /translation="MATAAPTKPRKTAKPAAAATKTPKAPAQASNLVGRVAQVIGAVV
                     DVAFEGELPPILAALETDNGGNRLVLEVAQHLGENVVRTIAMDSTDGLTRGQSVKTTG
                     SQIQVPVGPKTLGRIINVIGEPIDERGPIGSDKFASIHAEAPKFVDQSTEAAILVTGI
                     "
BASE COUNT         1300 a         2740 c         2539 g         1149 t
ORIGIN      
        1 gccgaaaggc tcgagcgctt cgatcaggcg ctccgttcgc tgcagggagt ggtcgagcag
       61 gaaccgctgt ccgacaccca gcggctgacg gtgttccgct tcggcgacat cggcgacatc
      121 gaaaccctgg cccgccggga aggcgttgcc ggattctata tcccgcgcgc ggcggggccc
      181 gtcgccttcg ttccggccaa gaacgcgatc ggcaagacga gcctgtaccg ggatcccgag
      241 atcgatgcgc agaccgtgtt cttccacgaa tatgcgcacc atttcatgta ccggcatttc
      301 accgccgctt acccgagctg gtaccgggag gcgttcgccg aggtcaattc gaccatcgag
      361 ctccaggacg acggcagctt caagctcggc tcgccgccgc aaagccgcgc cagcgccctg
      421 ctcggcggcc tcaattactc gatcaagcgc atgcttctga ccagcaaccg gccgaacttc
      481 gaggacgtct acgcccgcta cacttatggc tggctgctga cccattatct gaccttcggg
      541 ggcacccgga ccggccagct gcagaaatat ctgcgggcga tcaacgccgg caccgacgcc
      601 gcaaccgccg cccggcaggc gttcggcgac ctcgaccagc tcgaaagcga ggttattcgc
      661 tacaagggca ggaaccgcta tccgggcgcc atcgtccgtc cgggtagcct gcccaagccc
      721 aatgtcgcga tgcgcaagct tgggcgggac gaggaagcga tcatgcgcgt ccgggcgcgc
      781 tcggcgcgcg gggtcgacaa gaagacgtcg aaggatgtcg cggccgatgc ccgggcggtg
      841 gcggccaaat acacgggcag cctcgcggtg caggtcgcgc tggccgaggc cgagttcgat
      901 gccgagcggt tcgacgaggc ggagcgagcc gccgacgcgg cgctggcgat cgatcccaga
      961 tcgggcaagg cgctgctcta caaggggctg gtctatttga agcgcagcaa ggccgacccg
     1021 cggcattacg caaccgcgcg cagctggttc gggaaggcga gccgggtgga cccaggcgac
     1081 ccgagcccgc tgtacaatta ttatctgacc tacaagcagg ccggcgcccc gccgccgcaa
     1141 ctcgccgtcc aggggctcga gcgggcattc gagatcgccc cctacgacgc cggcctgcgg
     1201 gtgctgaccg cccgccagct gctcatcgac cgcaaggggg cgctcgctcg ccaggttctg
     1261 ctcccggtcg cgcattcccc gcacgaatcg aaatattcga aggcgctcaa cgaggtcgcc
     1321 gagctgatcg aggccaacaa attgggcgag gcgctggcca agctcaacgc gcgcctggac
     1381 aaggacgaga aggacgatga cgacgacgtg cgcgcgccgt cgccggtcgt cgaggccctc
     1441 tagaggtcga gagaccgctg tccggccgca cgctcggcct ccagcagcct tcgcttgcgc
     1501 tcgatgccgc cggcatagcc gccaaggctg ccgtcgctgc gcaccacccg gtggcacggg
     1561 atcaacaccg cgaccgggtt ggcgccgttg gccgagccgg tggcccggac cgcgccgggc
     1621 ttgccgaccg cggcggcgat ctgcgcgtag ctgcgcgtct cgcccggcgg gattttcctg
     1681 agctcgcgcc acaccgcttc ctggaacgcg gtcccggcga cgtcgattgg caggtccgga
     1741 gccgccgcgg ggcgctcgac cgcgcacagc gcgcccgcga cccattcgcc catcgacccg
     1801 tcgtcctcga ccagggtcgc gttggggaag acccgcatta gcgagcgctc gtcgtcctcg
     1861 aaggtcagcc ggcagatgcc cctggccgtc gccgcgacca gcagcggccc gagcgcgctg
     1921 gcgacgaccg tccagcggat ggtctcgccg cgcccgccgt cgcgccacgc ggacggggtc
     1981 atgccgagcc gctctttggc gtcggcatag aatcggctcg gcgccgaata gcccgaatcg
     2041 tagatcgcct cggtcacgct tttgtcctcc gtcagctcgg cctcggcgcg ccgggcccgg
     2101 agcgcccgcg catattgcgc cggcgacacg ccgacgtcgc gcgtgaacag ccgctggaaa
     2161 tgatgcagcg catagccgac cgcggcggcg agctctgcca ggctcggcgc ttcctccgcc
     2221 gtttcgatca gccgcacggc gcgggcgacc gcggcgcggt cgcggccgac ctcgtcgggc
     2281 ttgcagcgca ggcacggccg gtagccggcc gcccgcgccg cctgggggtc ggcgaagaac
     2341 tcgacatgct cgcgccgcgg ccgccgcgcc gggcagctcg gcttgcaata gatgccggtg
     2401 gtccggaccg ctccgatcag ccgcccgtcc aacgcccggt cgcggcgctc gaacgccgcc
     2461 caggcctggt tcgcatcgat ggttttcatc agcatgggac cgcgaatacg ccactccggc
     2521 gcatcgcgca tcccgctgct tgcggccaaa ccggtcagtt ggccgcggcc gctcctccgg
     2581 agcgttcgtc gacgacctcg tcgcgccggt aggacagcac cccgggcagg gccgtcagcc
     2641 ggtcgatctg gacgccgaag aaattgtcga tcttgccggc ggcccggtac gcggccgcct
     2701 cgcgcggcag gctcgccagg ctcgccggca ccgggtcggc cgggcgcatc ggctcgacgc
     2761 gcatcccggt cagcccgccc ttgcccggag tgaggccgtt ccagctcggc ttcaccttgt
     2821 cgcgcaaggc ctcggcgagc gggtagcgga acgagccgag atcggtgatc cggccgggcg
     2881 cggcgtcgaa gtcgaccgag cccatgcaca ggcagaagcc gagcatgctg ccgttgttgc
     2941 cctcgatcat ctggccgtag agccggtagg tgccgggatc gacggcgatc agccgcgcct
     3001 gcgacggctt gtcgaacacc cgtccgcccc acagggtcac gaacttgtcg gactcgatcg
     3061 ccttgaaggc gagattgtcg tccgtcggct cgaccggccg ctcgggcttg acctggccgg
     3121 cgcgcgcgtt ggcggacatc gcgttccagg ccttgatgtc gcgttcgtag ccggcgagct
     3181 tgcgggcata tttggaccgc gccttttcga gcgcggccga gcgctcggcc ttgtaggcct
     3241 cgacctcgtc cggccgcgcc tcgcgaagca gcttgacgtc gagattgagc ggcgagcgca
     3301 ccagcagata ggccttggcc gggtcgaggc tgatcgccgt tccggccttg aactgctgcg
     3361 aggaagccgg cttggcggcc gccggagcgt gcgcggcgaa cgcggccgcg gcggcggaaa
     3421 tgagcatcag gatacgcatc agaactcgag ctcctcggtc tggggcttgc cggcaatcgc
     3481 cgccttggcc tgcatttgaa ggagtttctg ccgctcgtcc tcggcaacgc cgctcaggcc
     3541 ctcctcgaaa ttgaacttgg tccacagctc cttgggcagg ccatgccggc tgtagccctt
     3601 gtagcccatg caggtgcgca tgttggtccg gcgcatgttg cggcgcagcg ccgagccgaa
     3661 gatggcgtcg gcgagcgcgc tcccgatcgc cccgccgagc gctccggcca tcgtcccggc
     3721 ataagggtaa gggaccgtcc cgccgttgag gtaggtgcgg attccgcggg tcagggaatc
     3781 gcattcgcgg atgtcgttgt aggcggttgc gaaatcggtc tcggagcgca cgaaatagaa
     3841 atatttgtcg tacccgtcgc ggtcgccggc agcttcggtg aagccgagtt ccggcagcgg
     3901 caccttggcc ggatcgacct cgatcggagc gagattgcga tcggccacct tttgcgcctg
     3961 ggccggcgcg ggcaacgcca tggccaatgc ggcaagcgct gccgcccggt ttcgaattgt
     4021 catgtgattt caaacccccc agcggcccga gacgcggccg ccggcttttt tgccgagccg
     4081 ccttcgagcc gtcaagacgg ctcttcggga ccacccgcct tgcatcgttt cagcccccct
     4141 gctaggggcg cgccaaccca tcggggcggg cgggtcgaag gccgtgcaac gccccgtttt
     4201 cgcatggggt catatctttt tttggagctt cgggcgcgtg gagacatccg gcggcattca
     4261 agccagctta gcagggcgct acgcctccgc tttgttcggc ctggcgcgcg acgaacggaa
     4321 gatcgacgcg gtcggccgga gcctcgacgc gcttgaccac gcgctggccg attcgcgcga
     4381 gttcgccgcg ctggtggcga gcccgctggt cggccgcgac gatgccgcca aggcgttcgc
     4441 cgccctcggc cccgagctcg ggctcgaccc gctgaccgtc aatttcctcg gcgtcctcgc
     4501 ccgcaacggc cgcaagcgcg agttgaaggc gatcatccgg gcgtatcggc gcatcgccgc
     4561 cgaccaccgc ggcgagatca gcgccgaagt cgtcagcgcc cacccgctca acgacgacca
     4621 ggtcgaagcg ctgaaggcgc agctcaggaa gcgcgccgga ggggcggacg tcacactcga
     4681 ctcccgggtc gaccccgaaa tcctcggtgg catcgtcatc aagctcggaa gtgagatgat
     4741 cgacgcttcc atccgcacca aactcaaccg actcgcgtct gcgatgaaag gctagttcat
     4801 ggacatccgc gccgctgaaa tctcccgcgt catccgcgat cagatcgcga atttcgcctc
     4861 cgactcggag gtctcggagg tcggcaccgc gctctccgtc ggcgacggaa tcgcccgcat
     4921 ccacggcctc gacaatgtcc aggccggcga gatggtcgag ttcgagaacg gcgtcaaagg
     4981 catggctttg aacctcgagg ccgacaatgt cggggtcgtc gtgttcggct ccgacgccga
     5041 gatcaaggaa ggctcgaccg tcaagcgcac cggcaccatc gtcgacgtgc cgatcggcaa
     5101 gggcctgctc ggccgcgtcg tcgacgcgct cggcaacccg atcgacggca agggcccgat
     5161 cgaggcgacc gagcgccgcc gcgtcgaggt caaggcgccc ggaatcatcc cgaggaaatc
     5221 ggtccacgag ccggtgcaga cggggttgaa ggcgctggac gcgctggttc cgatcggccg
     5281 cggccagcgc gagctgatca tcggcgaccg ccagaccggc aagaccgcgg tcgcgctcga
     5341 caccttcatc aaccagaagg cgatcaacgc gtcgggcaag gagagcgaga agctctactg
     5401 catctacgtc gccatcggcc agaagcgctc gaccgtcgcc cagatcgtcc gcgcgctcga
     5461 ggagaatggc gcgatggact attcgatcgt cgtcgccgcg accgcgtccg agccggcgcc
     5521 gctgcagttc ctcgcgccct acaccggctg cgcgatgggc gaatatttcc gcgacaacgg
     5581 aatgcacgcg gtgatcgtct atgacgattt gtccaagcag gcggtcgcct accgccagat
     5641 gtcgctgctg ctgcgccgcc cgccgggccg cgaagcctat ccgggcgacg tcttctatct
     5701 ccacagccgc ctgctcgagc gcgccgccaa gatgaacgag gacaatggcg ccggctcgct
     5761 gaccgccctg ccgatcatcg agacccaggc cggcgacgtc tcggcctaca tcccgaccaa
     5821 cgtgatctcg atcaccgacg gccagatctt cctcgagacc gacctgttct tccagggcat
     5881 ccgcccggcg atcaacgtcg gcctgtcggt cagccgcgtc ggctcggccg cccagaccaa
     5941 ggcgatgaag aaggtctccg gctcgatcaa gctcgagctc gcccaatacc gcgagatggc
     6001 ggccttcgcc cagttcggct cggacctcga cgcctcgacc cagcgcctgc tcgcccgcgg
     6061 cgcccgcctg accgagttgc tcaagcagcc gcaattctcg ccgatgccgg tcgaggagca
     6121 ggtcgcgtcg atcttcgccg gcacccaggg gttcttggat tcggtagagg tcagggacgt
     6181 cacccgctac gaagcggcgc tgctcagcta tctgcgcgcc gagcacgccg acgtcctgaa
     6241 gacgatccgc gacagcaagg cgctcgacga cgacacggcg aaaaagctca aggacgcgct
     6301 gaccaagttc ggcaagcagt tcgcgtaaag cccgatggca agcctcaagg ccctcaagat
     6361 ccgcatcggc tcggtcaagt cgacgcagaa gatcaccaag gcgatgaaga tggtcgccgc
     6421 ggccaagctg cgccgcgcgc agcagggcgc cgaggcgggc cgcccctacg cgacccggat
     6481 ggccgacgtc gtcgcctcga tcgcctcggg ggtcacggtc agcccgcaaa gccccaagct
     6541 gctcgccggc accggcaagg accaaaccca tctgatcgtg ctcgccaccg gcgaccgcgg
     6601 gctcgcgggg gcgttcaaca gcaacatcgt ccgggccgcc cgccgcaggg ccgacgagct
     6661 gatcgaggac ggcaagacgg tgctgttcta cctcgtcggg cgcaagggcc ggccggtgat
     6721 ccagcggctc tacccgaagg cgatcatcgc ccagcacgac accaccgcca tgaaggcgcc
     6781 cggctacgcc gacgcgcagg cgatcgccgc cgacatcatc gaccgcttca tcaccgacgg
     6841 catcgacgtc gtccacctcg cctacgcgca gttcaagtcg acgctcgtgc aggagccggt
     6901 ggtgcagcag atcatcccgg tgcctagagc tgcgaacgac acccgtccgg cgagcgcatc
     6961 gaatgtcgcg gtggaatatg agcccgacga ggaagcgatc ctggaagcgc ttctgccgcg
     7021 caacatcgcg gtgcagatct accgcgccct gctcgagaac caggccggct tctacggctc
     7081 gcagatgacc gcgatggaca atgcgacccg caacgccggc gacatgatca accgcctgtc
     7141 gatccagtac aaccgccagc gccaggcagc gatcaccacc gagctcgtcg aaatcatctc
     7201 gggcgcggaa gcgctctaac ttgaccgaac aacgcaagga tacacccatg gccaccgccg
     7261 ccccgaccaa gccccgcaag accgcgaagc cggccgccgc cgcgaccaag acccccaaag
     7321 cgccggcaca ggcgagcaac ctcgtcggcc gggtcgcgca ggtgatcggc gcggtcgtcg
     7381 acgtcgcctt cgagggcgag ctgccgccga tcctcgccgc gctcgagacc gacaacggcg
     7441 gcaaccgcct ggtgctcgag gtcgcgcagc atttgggcga gaacgtcgtg cgcaccatcg
     7501 ccatggattc gaccgacggc ctgacccgcg gccagtcggt caagaccacc ggctcgcaga
     7561 tccaggtgcc cgtcggcccc aagacgctcg gccggatcat caacgtcatc ggcgagccga
     7621 tcgacgagcg cggcccgatc ggcagcgaca agttcgcctc gatccacgcc gaagcgccga
     7681 agttcgtcga ccagtcgacc gaagcggcca tcttggtcac cggcatca
//
LOCUS       DASTZO010000141         4329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_273020,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000141 DASTZO010000000
VERSION     DASTZO010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4329
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273020"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1090
                     /gene="lpdA"
                     /locus_tag="VFU20_03990"
     CDS             <1..1090
                     /gene="lpdA"
                     /locus_tag="VFU20_03990"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006954650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEU5285659.1"
                     /translation="LFKKNKVEWLKGAASFESPNRVKVGDRTVSAKNVVIATGSSVTP
                     LPGVEVDNEKGRIVDSTGALALPQVPERLVVIGGGVIGLELGSVWRRLGSQVTVVEYL
                     DEILPGMDGEVRKECAKIFKKQGFEIRTSSKVTSAKVNGASVALTVEPAKGGAAETIE
                     ADAVLVSIGRRPNTDGLGLDAIGLKVNNRGQIETDPEFRTAVPGVWAIGDVIPGPMLA
                     HKAEDEGIAVAENIAGLTGIVNHAVIPNVVYTHPEIAGVGLTEEEARQEGEVKTGKFP
                     FLANSRAKTNRDTDGFVKVIADARTDRVLGVWMVGSLAGTMIAQAAQAMEFGATSEDI
                     AYTCHAHPTHSEALKEAAMAVTGKPIHI"
     gene            1104..1799
                     /locus_tag="VFU20_03995"
     CDS             1104..1799
                     /locus_tag="VFU20_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEU5285660.1"
                     /translation="MITRSRLRRWHVWLGWVIALPILFWVISGLVMVSKPIEEVRGEH
                     LVREPGPIRLALPPVPPAVAGLPMKSMSLEQRADGPRWVIALADGTSRLADPATGALL
                     PPLSAADAVREVTARYTGKARVKSATRTDPDKPPLELRRPVAAWLVAMDDDTRFYVDS
                     GSGQIVARRTPWWRFYDFMWGLHIMDLKTREDMHNPLVIGFAIVALVMSILAMILLPL
                     TINRRRRRAIRQD"
     gene            complement(1827..3689)
                     /gene="sppA"
                     /locus_tag="VFU20_04000"
     CDS             complement(1827..3689)
                     /gene="sppA"
                     /locus_tag="VFU20_04000"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEU5285661.1"
                     /translation="MKFVRAIWKLLVGIKDALVLLFMLLFFALLYAALSARPDPVTDG
                     VLALDLDGTVVEQPARAELSELAAGGSRLRQFRLRDLVAALDEAREDNRVKAVALDLE
                     GFLGGGQTAMADLADALRRVRSSGKPVITYSVGYSDDAYQLAAASSEVWLHPLGAVAI
                     TGPGGSNLYFKGLLDKLGVTANVYRVGTYKAAVEPFIRNDMSPEAEENYQAFGDALLE
                     SWREGVLRARPRAKLDAYVRDMPAAVDAAGGDLARAALTAGLVDRLADRRAFEARLAE
                     LGGEDEGAPGGYSRIKLDSYIAERVERDGGGPIGVVTVAGMIVDGRAPEGTAGGDSIA
                     KAIEKGLSDGGLKALVVRVDSPGGSVLASERIRQAILAAKERKLPVVVSMGNVAASGG
                     YWVATAADYIFAEPSTITGSIGVFGILPSFQGSLEKLGMGADGIKTTPLSGEPDLLKG
                     PSPEVNRLIQASVESIYRHFLGVVSRSRGKPPAEIDRIAQGRVWDGGTARQLGLVDGF
                     GGMDEAIAKAAQLARLGEGQRGVRYLERPQSLTDSLLEMLANEELDDGTAPTDAFAVI
                     GGSPERELAAAIAELRSILSGSRIQALCLECGPVAPVQVRARDVSLLAALADWF"
     gene            3875..4162
                     /gene="groES"
                     /locus_tag="VFU20_04005"
     CDS             3875..4162
                     /gene="groES"
                     /locus_tag="VFU20_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU5285662.1"
                     /translation="MGFRPLHDRVLVRRVEAEEKTAGGIIIPDTAQEKPQEGEVISVG
                     SGSRSDDGKVTPLDVKAGDRILFGKWSGTEVKIAGEDLIIMKESDILGVVG"
BASE COUNT          711 a         1479 c         1510 g          629 t
ORIGIN      
        1 cctgttcaag aagaacaagg tcgagtggct gaagggcgcc gcgtcgttcg agtcgcccaa
       61 ccgggtcaag gtcggcgacc gcaccgtcag cgcgaagaac gtggtgatcg cgaccggctc
      121 gtcggtgact ccgctgcccg gagtcgaggt cgacaatgag aagggccgga tcgtcgattc
      181 gaccggcgcg ctcgccctgc cgcaggtgcc cgagcggctg gtggtgatcg gcggcggggt
      241 gatcgggctc gaattgggct cggtgtggcg ccgcctcggc agccaggtga cggtggtcga
      301 gtatctcgac gagatcctgc ccgggatgga cggcgaagtg cgcaaggaat gcgcgaagat
      361 cttcaagaag cagggcttcg agatccgcac ctcctccaag gtcacaagcg ccaaggtcaa
      421 cggcgccagc gtcgccttga cggtcgagcc ggcgaaaggc ggcgcagcgg agaccatcga
      481 ggccgacgcg gtgctggtgt cgatcggccg ccgccccaac accgacgggc tcgggctcga
      541 cgcgatcggg ctcaaggtca acaaccgcgg ccagatcgag accgaccccg agttccgaac
      601 cgcggtgccg ggcgtgtggg cgatcggcga cgtgatcccc gggccgatgc tcgcccacaa
      661 ggccgaggac gaggggatcg cggtcgccga gaacatcgcc gggctcaccg ggatcgtgaa
      721 ccacgcggtg atccccaacg tcgtctacac ccatcccgaa atcgccgggg tcggattgac
      781 cgaagaggaa gccaggcagg agggcgaggt gaagaccggc aagttcccgt tccttgccaa
      841 cagccgcgcc aagaccaacc gcgacaccga cggcttcgtc aaggtgatcg ccgacgccag
      901 gaccgaccgg gtgctcggcg tgtggatggt cgggtcgctg gcgggcacga tgatcgccca
      961 ggccgcccag gcgatggagt tcggagccac gtccgaggat atcgcctata cctgccacgc
     1021 ccacccgacg cacagcgaag cgctgaagga agcggcgatg gcggtgaccg gcaagccgat
     1081 ccacatctag gaagcgacgc caaatgatca cccgttcccg cttgcgccgc tggcacgtct
     1141 ggctcggctg ggtgatcgcg ctgccgatcc tgttctgggt gatctccggg ctggtgatgg
     1201 tgtcgaagcc gatcgaggag gtgcgcggtg agcatctggt ccgcgagccg gggccgatcc
     1261 ggctggccct cccgcccgtc ccgccggccg tcgccggcct gcccatgaag tccatgtcgc
     1321 tcgagcagcg agcggatggg ccgcgctggg tgatcgccct ggccgacggg accagccgcc
     1381 tggccgatcc ggcgaccggc gccctgctgc cgcccttgtc cgccgccgac gcggtgcgcg
     1441 aggtgacggc gcgctacacg ggcaaggccc gggtcaagtc cgcgacccgg accgacccgg
     1501 acaagccgcc gctcgagctc cgccgcccgg tcgccgcctg gctggtggcg atggacgacg
     1561 acacccgttt ctacgtcgat tccggatcag ggcagatcgt tgcccggcgc accccgtggt
     1621 ggcggttcta cgacttcatg tgggggcttc acatcatgga cctgaagacc cgcgaggaca
     1681 tgcacaaccc gctggtcatc ggctttgcga tcgtcgcgct ggtgatgagc atcctggcga
     1741 tgatcctgtt gccgctgacc atcaaccgcc gccggcgccg ggctatccgg caggactgaa
     1801 cggcggatcg gcggccgccg ccgtcatcag aaccagtcgg cgagcgcggc gaggaggctg
     1861 acgtcgcggg ccctgacctg caccggggcg accgggccgc attcgaggca aagcgcctgg
     1921 atccgcgagc ccgacaggat cgagcgcagc tcggcgatcg cagccgccag ctcgcgttcc
     1981 ggcgagccac cgatcaccgc gaacgcgtcg gtcggagcgg tgccgtcgtc gagctcctcg
     2041 ttggccagca tctcgagcag gctgtcggtc aggctctgcg gccgctcgag gtagcggacc
     2101 ccgcgctggc cttcgccgag cctggcgagc tgggccgcct tggcgatcgc ttcgtccatc
     2161 ccgccgaagc cgtcgaccag gccgagctgg cgggcggtgc cgccgtccca cacccggccc
     2221 tgggcgatcc ggtcgatctc ggccggcggc ttgccgcgcg agcgcgagac gaccccgagg
     2281 aaatgccggt agatcgattc caccgaggcc tggatcagcc ggttgacctc gggcgacggg
     2341 cccttgagca ggtccggctc gcccgacagc ggggtcgtct tgatcccgtc ggcccccatt
     2401 ccgagcttct ccagcgagcc ctggaagctc ggcaggatcc cgaacactcc gatcgacccg
     2461 gtgatcgtcg acggctcggc gaagatgtag tcggcggcgg tcgcgaccca atagccgccg
     2521 gacgcggcga cattgcccat cgacacgacc accggcagct tcctctcctt ggcggcgagg
     2581 atcgcctggc ggatgcgctc ggacgccagc accgatccgc cggggctgtc gacgcgcacc
     2641 accagcgcct tcaggccgcc gtcggagagg cccttctcga tcgccttggc gatgctgtcg
     2701 ccgccggcgg tgccttcggg cgccctgccg tcgacgatca tcccggcgac ggtgacgacg
     2761 ccgatcgggc cgccgccgtc gcgctcgacc cgctcggcga tgtaggaatc gagcttgatc
     2821 cggctgtagc cgccgggcgc gccctcgtcc tcgccgccga gctcggcaag gcgggcctcg
     2881 aacgcgcgcc ggtcggcgag ccggtcgacc agcccggcgg tcagagctgc gcgggcgagg
     2941 tcgccgccgg cggcatcgac cgccgccggc atgtcgcgca cgtaggcgtc gagcttggcc
     3001 ctggggcgcg cgcgcaggac gccctcgcgc cagctttcga gcagcgcgtc cccgaacgcc
     3061 tggtagttct cctcggcttc gggcgacatg tcgttgcgga tgaacggctc gaccgcggcc
     3121 ttgtaggtgc cgacccggta gacgttggcg gtgaccccga gcttgtcgag caggcccttg
     3181 aaatagaggt tcgacccgcc cgggccggtg atggcgacgg cgcccagcgg gtgcagccag
     3241 acctcggacg acgcggccgc gagctggtag gcatcgtcgc tatagccaac cgagtaagtg
     3301 atcaccggct tgcccgacga gcgcacccgg cggagcgcat cggcgaggtc ggccatggcc
     3361 gtctggccgc cgcctaggaa gccttcgagg tcgagcgcga ccgccttgac cctattgtcc
     3421 tcgcgcgcct cgtcgagcgc cgcgacgagg tcgcgaagcc ggaattgcct caatcggctg
     3481 ccgcccgcgg cgagctccga cagctccgcc cgggccggct gctcgacgac cgtcccgtcg
     3541 aggtcgagcg cgagcacccc gtcggtcacc gggtccggcc gcgccgacag cgccgcgtag
     3601 agcagggcga agaacagcag catgaacaac agcaccaggg cgtccttgat gccgaccagc
     3661 agcttccaga tcgcgcgcac gaatttcatc gcggtctacc tcggataatg cccgcgcagc
     3721 gctagcggag gtccctcgcc cgcgccaaat cttttcgacc ggcaggcgtt gacgcggcga
     3781 gcgggggcaa ccatatgcgc ggcatcgctg gcactctcat gacgagagtg ccaagcgcat
     3841 cgatgagcaa cattttgaca aggggctaaa ggctatgggt ttcaggccgc ttcacgatcg
     3901 agtcctcgtc cgccgcgtcg aggccgagga aaaaaccgcc ggcgggatca tcattcccga
     3961 taccgcgcag gaaaagccgc aggaaggcga ggtcatttcg gtcggctcgg gctcgcgctc
     4021 ggacgacggc aaggtgactc cgctcgacgt caaggccggc gaccggatcc tgttcggcaa
     4081 atggtcgggc accgaggtca agatcgcggg cgaagacctg atcatcatga aggaaagcga
     4141 catcctcggg gtggtcggct gatttttcca acacggaatt tgatttgaaa gggagccgaa
     4201 catggcagcc aaggacgtga aattttcgcg cgacgcgcgc gagcggattc tgcggggcgt
     4261 cgacatcctc gccgatgcgg tcaaggtcac cctggggccc aagggccgca acgtgatcct
     4321 cgacaagag
//
LOCUS       DASTZO010000142         4933 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_481902,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000142 DASTZO010000000
VERSION     DASTZO010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4933)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4933)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4933
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481902"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..583
                     /locus_tag="VFU20_04010"
     CDS             <1..583
                     /locus_tag="VFU20_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285663.1"
                     /translation="APAPAPAPAPLQRAPAPRPAARAGIVSTRLRPWLEIEFTPGRCV
                     VEDDKATIQFDIAVFNSGSAPARDVLVEASMFNAGPAQDEEIGAFFAHPVAKGDRIPV
                     IPPLKRVALKSAVSLTRDRLREFEVAGRRLFVPLVGFNALYRWSGGDGQTSASYLVGR
                     DTEGEKMAPLRLDLGPRIFRGLGAREHTVRLRK"
     gene            complement(841..1458)
                     /locus_tag="VFU20_04015"
     CDS             complement(841..1458)
                     /locus_tag="VFU20_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HEU5285664.1"
                     /translation="MVRTAETIRQSVEALAALEPAFATVLERHGEPQPRVSEPGAHTL
                     LRTIVGQQVSVAAARSMWAKLEAAYGSPPDLVRILEAGDDELRLAGISRQKAGYARSL
                     AGLVLSGELDLGNLPADDEEAISLLTRIKGIGRWSAEIYLLFAEGRRDVWPAGDLAVQ
                     VAIGRLLGLDERPSEKRLRELGEAWRPHRGAAAVLAWHSYNSAVL"
     gene            complement(1458..2198)
                     /gene="rlmB"
                     /locus_tag="VFU20_04020"
     CDS             complement(1458..2198)
                     /gene="rlmB"
                     /locus_tag="VFU20_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEU5285665.1"
                     /translation="MTRHKRSHPHGSPNRPRLWGKHAVAAALDNPERKVLRAWATRDA
                     ASFMRFPKEVPLTFADVADLGRLVPHDAPHQGVVVEVEPLEGAWLGDLLGNAGERSVL
                     LVLDQVTDPHNVGAILRSAAAFGAIGIVTQDRHSPPESGALAKAASGALERVPWARVV
                     NLARALEEIGEAGFWRVGLAGEADMELKDALGPPRIALVLGAEGPGLRPNTREHCDAL
                     ARLPISAAVDSLNVSNAAAVALYAASIA"
     gene            complement(2271..2720)
                     /locus_tag="VFU20_04025"
     CDS             complement(2271..2720)
                     /locus_tag="VFU20_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285666.1"
                     /translation="MIPRYYIFLAILLLSCGYALWRGRRDERIVASICLLATVATRFA
                     ISPLSVRYTGLEFGLLFIDLVMLAAFVSIALRSERFWPLWIAGLQLTNSLSHLMKAIE
                     LDLLPVAYRAAAVFWSYPILLVLAIGTWRGQRYFAEERSTPPREQRA"
     gene            complement(2767..3111)
                     /locus_tag="VFU20_04030"
     CDS             complement(2767..3111)
                     /locus_tag="VFU20_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285667.1"
                     /translation="MRKQQLENAAFEVATQVRTVEETIDSALAEMAELQSRVMRANAI
                     AHVGVGAIHGTLEQLAAAISGLVQTRGSIVACHAALVDAKGKVPGLRTVSWGDAEDCP
                     EEIATHNLRVVA"
     gene            complement(3185..3757)
                     /locus_tag="VFU20_04035"
     CDS             complement(3185..3757)
                     /locus_tag="VFU20_04035"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5285668.1"
                     /translation="MANAMAGSHDPADIELMAASRVARLSPGQLDCLRLVAEHLSSKE
                     IAAELHISPHTVDQRIRGALHILGVERRTHAARLVARHCGPYQRMIHQSPYVEPDRST
                     VHPMGTVSNQIRHADRAGGAGGAGFITEQRPTSFRPSLQMPFATRSNPRNEMSVAQRL
                     LWIVLISTGAAFSAGMYLAGLESLARLLKG"
     gene            complement(3904..3989)
                     /locus_tag="VFU20_04040"
     tRNA            complement(3904..3989)
                     /locus_tag="VFU20_04040"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3953..3955),aa:Tyr,seq:gta)
     gene            complement(4191..4264)
                     /locus_tag="VFU20_04045"
     tRNA            complement(4191..4264)
                     /locus_tag="VFU20_04045"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4230..4232),aa:Gly,seq:tcc)
     gene            4542..>4933
                     /locus_tag="VFU20_04050"
     CDS             4542..>4933
                     /locus_tag="VFU20_04050"
                     /inference="COORDINATES: protein motif:HMM:NF014884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285669.1"
                     /translation="MTHNPSLAPRREEAVGPFPSDPLDPAAELAVAQLLEGRDLDRQQ
                     AQALFAQIVEGRLPPPLMAAAFVALRMKGETAEELTGAALSLRAAAMPFARPYYLFAD
                     SCGTGGDYSGSINVSTAAGLVVAACGLPV"
BASE COUNT          774 a         1701 c         1673 g          785 t
ORIGIN      
        1 cgcgccggcg ccggcgccgg caccggcacc gctgcagcgc gcgcctgcgc cgcggccggc
       61 tgcgcgggcc ggaatcgtct cgacccggct tcgaccgtgg ctcgagatcg agttcacgcc
      121 cgggcgctgc gtcgtcgagg acgacaaggc cacgatccag ttcgacatcg cggtgttcaa
      181 ctccggcagc gccccggcgc gcgacgttct ggtcgaggcg agcatgttca atgccggccc
      241 ggcgcaggac gaggagatcg gcgccttctt cgcgcacccg gtggccaagg gggaccggat
      301 cccggtcatc ccgccgctca agcgggtggc cctgaagagc gccgtcagcc tgacccgcga
      361 ccggctgcgc gagttcgagg tcgcggggcg gcggctgttc gtgccgctgg tcgggttcaa
      421 cgcgctctac cgctggagcg gcggcgacgg gcagacctcg gccagctacc tggtcggccg
      481 cgacaccgag ggcgagaaga tggccccgct gcgcctcgac ctcggcccgc gaatcttccg
      541 cggcctcggc gcccgcgagc acaccgtccg cctgcgcaaa taggctgaat acgttttctc
      601 cgccccggcg ggattgaata tgccttggtc cgggaccagt tgcccgccct cgagcggaac
      661 gtgacatgat ccatcccatc catatcctcg cgctgagcca gcccggattc gtccagatgc
      721 aggtgcgcaa cgtcttttgc ggcgggaacg gttgcgcccg gcgccgcgag agcttttggc
      781 gaggctgggg ctttcctgaa ttctagccgg cgcgaagccc agccgccgcg ccgcggagcc
      841 ctacaggacc gcgctgttgt agctgtgcca cgcgagcacg gccgcggctc cgcggtgcgg
      901 gcgccaggcc tcgccgagct cgcgcagccg cttttcgctc ggccgctcgt caagcccgag
      961 caggcgcccg atcgcgacct ggacggcgag gtcgccggcc ggccagacgt cgcgccggcc
     1021 ttcggcgaac agcaaataga tttccgccga ccatcggccg atgcccttga tccgggtcag
     1081 cagggagatc gcttcctcat cgtccgccgg caggttgcca aggtcgagct cgcccgacag
     1141 cacgagcccg gcgaggctgc gcgcgtaacc cgctttctgg cgcgagatcc cggccagcct
     1201 cagctcgtcg tcgcccgcct ccaggatgcg cacgaggtcg ggcggtgagc cgtaggcggc
     1261 ttcgagcttg gcccacatcg agcgggcggc cgcgacgctg acctgctggc cgacgatcgt
     1321 ccggagcaga gtgtgcgcgc cgggctcgct aacccggggc tgcggttcgc catgccgctc
     1381 gaggacggtg gcgaacgccg gttcgagtgc ggccagcgcc tcgacgctct ggcggatcgt
     1441 ctcggcagtc cggaccatca ggcgatgctg gccgcgtaga gcgcgaccgc ggccgcgttg
     1501 gagacgttga ggctgtcgac cgccgcgctg atcggcaggc gcgccagcgc gtcgcaatgc
     1561 tcgcgggtgt tgggcctcag gcccggccct tcggcgccga ggaccagcgc gattcgcggc
     1621 gggccgagcg cgtccttgag ctccatgtcg gcctcgccgg ccagcccgac tcgccagaag
     1681 ccggcctcgc cgatctcctc gagcgcccgg gcgaggttga cgacccgcgc ccacggcacc
     1741 cgctcgagcg cgcccgaggc cgccttggcc agggccccgc tttcgggcgg cgagtggcgg
     1801 tcctgggtga cgatcccgat tgccccgaac gccgccgccg agcgcaggat ggccccgacg
     1861 ttgtgggggt cagtgacttg gtcgaggacc agcagcaccg agcgctcgcc cgcgttcccg
     1921 agcaggtcgc cgagccaggc tccctcgagc ggctcgacct cgacgacgac accctggtgc
     1981 ggggcgtcgt gcgggaccag ccggccgagg tcggcgacat cggcgaaggt cagcggcacc
     2041 tctttgggga agcgcatgaa ggacgccgcg tcgcgcgtcg cccaggcgcg cagcaccttg
     2101 cgctccggat tgtcgagcgc cgccgcaacc gcatgcttgc cccacaggcg cggacgattg
     2161 ggagagccgt gcggatgcga gcgcttgtgc cttgtcattc ggcgctcatg gaagtcgcgg
     2221 cgtttggagg caagccactg acggcggcgg ccgtcgccgc cggcggaggg tcaggcccgc
     2281 tgttcccgtg gcggcgttga ccgctcctca gcgaaatagc gctgaccgcg ccaggttccg
     2341 atcgccagca ccagcaggat cggatagctc cagaacaccg cggcggcgcg ataagcgacc
     2401 ggcagcaggt ccagctcgat cgccttcatc aggtgcgaca ggctgttggt cagctgcagc
     2461 ccggcgatcc acaagggcca gaagcgctcg gagcgcaggg cgatggagac aaacgccgcc
     2521 agcatgacca gatcgatgaa cagaagccca aattccagcc cggtgtagcg cacgctcaat
     2581 ggcgagatgg caaagcgggt cgcgaccgtg gcgagcaggc agatcgatgc cacgatccgc
     2641 tcatcccgcc gaccgcgcca cagggcatag ccgcacgaca gaagcaggat cgcgaggaaa
     2701 atataatatc tcggaatcat ggcaggcgga ccggaccaca ttgctgtggc ccggtcaaaa
     2761 cccttgttag gcgacgacgc gaaggttgtg ggtggcgatc tcttccgggc aatcctcggc
     2821 atcgccccag gacaccgtgc gaagccccgg gaccttgccc ttggcatcga ccagcgccgc
     2881 atggcaggcg acgatggagc cgcgggtctg caccaggccg ctgatggcgg cggccagctg
     2941 ctccagggtc ccgtggatcg cgccgacccc gacatgagcg atcgcattgg cgcgcatgac
     3001 ccggctctgc agctcggcca tctcggcgag tgcggagtcg atcgtttcct cgacggtgcg
     3061 gacctgggtg gcgacttcga acgcggcgtt ctcaagctgt tgtttgcgca tgactgctcc
     3121 tttccgcggg cgatgaggat cgcgcggata tggttgccga aagaagaaga gagttggttt
     3181 tccgctagcc cttgagcagc ctggcgagac tttcgagccc ggcgagatac atgcccgccg
     3241 aaaatgcggc cccggtcgag atcaacacga tccagagcaa ccgctgcgcg acgctcatct
     3301 cattccgggg gttgctcctc gttgcgaacg gcatctgcag ggagggtcgg aatgacgtgg
     3361 gcctctgctc ggttatgaaa cccgcgcccc cggccccccc tgcacggtca gcgtgccgaa
     3421 tctgattact gaccgtgccc atcgggtgga cggtcgagcg atcaggctca acatacggcg
     3481 attgatgtat catacgctga tatgggccgc agtgccgggc gacgagccgg gccgcgtggg
     3541 tgcggcgctc gactcccaag atgtggagcg cgccgcggat gcgctggtcg acggtgtggg
     3601 gcgagatgtg gagctcggcc gcgatttcct tggatgacaa atgctcggcc accagccgca
     3661 ggcagtcgag ctggcccgga ctcagccgcg caacccggct tgctgccatc aactcaatgt
     3721 ctgccggatc atgcgatccc gccatggcgt tggccacccg agcttccttc caaacacaat
     3781 cacacgggac tcggcaattg tgtccgagtc agtagtgact taggccaaaa cggtttgcac
     3841 cgccataagt gcataactaa tgttaagttt ttggcgggcg agggacggcg ggccgctcgg
     3901 ccgtggtgga gagggctgga ttcgaaccag cgtacgcttg cgcgggcaga tttacagtct
     3961 gctgccttta accactcggc cacctctcca ggcagggcag gcggcgcaca tggccacgag
     4021 ggtctgcgct gtcaattggc ccacgagccg gaatcgccgg cgctgcgttt cgcccctcgc
     4081 gaccggccaa acgctccaaa gcttcgacat gcccccggca tgtcgggggt cgccgcttcg
     4141 ctgcgctacg cgcgctccaa agcttcgaca tgcccccggc atgtcggctt tggagcgggt
     4201 gaagggaatc gaaccctcgt cgtaagcttg gaaggcttct gctctaccat tgagctacac
     4261 ccgcacgtcc agccggacgc gcccaatgcc attgcgggct cgcaggcgtc aaccgcttgc
     4321 cgccgccgcc aaccaggcga acgtcttgac gctgctcgat ttcatcgcct acccaaggac
     4381 tcgatgaagc acctggccgc cattgccgga aacgcccgcc gctggtggcg cgccgcgacg
     4441 cacgcggcgc gatggcgcga ctgatcaagg caatctagcc cagatccggt ctctcccgcc
     4501 cgcccgcacg gcgggctttt tcatttcagg tgctgttttc catgacccac aatccatcgc
     4561 tcgcgccgcg ccgcgaggaa gcggtcggtc cgttcccttc cgatccgctc gacccggcgg
     4621 ccgaactggc cgtcgcccaa ttgctcgagg gccgtgatct cgaccggcag caggcgcagg
     4681 cgctgttcgc gcaaatcgtc gaggggcggc tgccgccgcc gctgatggcc gcggccttcg
     4741 tcgcccttcg gatgaagggc gaaacggccg aggaattgac cggtgccgct ttgtccctgc
     4801 gcgccgccgc aatgccgttc gcccggccct attatctgtt cgccgacagc tgcgggaccg
     4861 gcggcgacta ttcgggctcg atcaacgtct cgaccgccgc cggcctggtc gtggcggcct
     4921 gcgggcttcc ggt
//
LOCUS       DASTZO010000143         5829 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_926010,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000143 DASTZO010000000
VERSION     DASTZO010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5829
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926010"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..2710)
                     /gene="acnA"
                     /locus_tag="VFU20_04055"
     CDS             complement(29..2710)
                     /gene="acnA"
                     /locus_tag="VFU20_04055"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEU5285670.1"
                     /translation="MIPTGQDSLDTRSTLTVGGKSYDYFSLAKAAEALGDVSRLPFSM
                     KVLLENLLRFEDGATVTRDDLQCMVDWQKERRISREIQYRPARVLMQDFTGVPAVVDL
                     AAMRDAMKALGGDPQKINPLVPVHLVIDHSVMVDEFGTPRAFGKNVELEYARNGERYE
                     FLKWGASAFDNFQVVPPGTGICHQVNLEYVAQCVWTSEDQSGAMLAYPDTLVGTDSHT
                     TMVNGLGVLGWGVGGIEAEAAMLGQPISMLIPEVVGFRLDGELREGITATDLVLTVTQ
                     MLRAKGVVGRFVEFYGPGLGSLSLADRATIANMAPEYGATCGFFPVDERTCEYLRLTG
                     RSDERIELVRAYCKALGLWRDDGSPEPLFTDTLELDMTSIEPSLAGPRRPQDRVLLSD
                     VDDQFNAELETTYKKHNDPRVAVEGENYDLGNGDVVIAAITSCTNTSNPSVLVAAGLV
                     ARKAREKGLTSKPWVKTSLAPGSKVVSDYLDASGLSADLNALGFDLVGYGCTTCIGNS
                     GPLAEPISRAIAEHDLVAVSVLSGNRNFEGRVSPDCRANYLASPPLVVAYALKGTVRS
                     DLAGEPIGTATNGEPVFLKDIWPSNEEVRALIDEHLHSHMFTSRYGDVYAGDERWRAI
                     EISGGETYSWPPQSTYIQNPPYFTDMTMTPVPPGDIAGARALALFGDSITTDHISPAG
                     AIKPDSPAGKYLVEQGVPRAEFNSYGARRGNHQVMMRGTFANIRIRNRMLDNVEGGFT
                     RYAPTGETLPIYDAAMRYMESGTPLVVIAGKEYGTGSSRDWAAKGTVLLGVRAVIAES
                     FERIHRSNLVGMGVLPLQFKDGDSADSLGLTGKEKYSIAGIAELNPRQDVEVRVFPDE
                     GEEFSFTARCRIDTYNELEYFKAGGILHYVLRRLAAA"
     gene            complement(2784..4106)
                     /locus_tag="VFU20_04060"
     CDS             complement(2784..4106)
                     /locus_tag="VFU20_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEU5285671.1"
                     /translation="MANDPTSRAAAAEPPSRRKAFKKPQIRLRDIFGPGLITGASDDD
                     PSGIATYSQAGAQFGYGLGWTMIFTYPLMSSVQMISARIGRTTGHGIAGNLRRHYPAW
                     LTTSLVSLLLVANIINIGADLGAMADATALVSGLPAPIFLLAFAAFCALSEVFMRYGN
                     YVRVLKWLTLALLAYVITLFLVDVPWSEALRGLVIPEVALNEAALIMIVAIFGTTISP
                     YLFFWQASEEAEDVHEIDGRQPLKCAPQDGRAELKRIEVDTFAGMAASNLVALAIILT
                     AAATLNANGITDIDTSAEAAAALEPVAGVYASTVFALGIVGTGLLAVPVLAGSAAYAL
                     GEALRWPVGLDRKPRRAKAFYGTIVAATAIGALMNFTPIDPIKALYWAAVINGVVAVP
                     VMAVMMLMAQRKSIMGVFTIVGSLRLFGWIATAAMAAAVAAMLWSIAG"
     gene            4268..4492
                     /locus_tag="VFU20_04065"
     CDS             4268..4492
                     /locus_tag="VFU20_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285672.1"
                     /translation="MAHDREYFRRRAAEARSASLRTDDGEGIEVAGHLALAYAALARR
                     RGAPARPDAPDAVDHADASVADAVPLMLGD"
     gene            complement(4503..5474)
                     /locus_tag="VFU20_04070"
     CDS             complement(4503..5474)
                     /locus_tag="VFU20_04070"
                     /inference="COORDINATES: protein motif:HMM:NF013694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEU5285673.1"
                     /translation="MLRAYGPDCDGSIVDPHHGPIPPGATWIDLEEPTREEERLVEQL
                     IGHNVPTEQELAEIEPSSRLFERDGALYMTLSALFGVSEGRPATTPIGFVLAGNRLVT
                     VRYATPKPLLAFGEHVLREPVLARDALTVLVRLLDAIIERLADELEASGAEIEKISNQ
                     IFGRDMDERRIPARRLTALLTRIGRAQSLLAKIRETAVSTGRLVSFVSASEPIKANDI
                     ARGHLASLMTDVTSLIDHSGFLGDNLIFLLDAALGLISVEQNAAMKLFSWAALIFLPP
                     TLVAGIYGMNFEHMPELDWLYGYPIALVLILASAVLPILYFKRRGWI"
     gene            5540..>5829
                     /locus_tag="VFU20_04075"
     CDS             5540..>5829
                     /locus_tag="VFU20_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2585 family protein"
                     /protein_id="HEU5285674.1"
                     /translation="MPTRSRAALAALLILAATAAVLLAMGRPAICACGTVELWGAVGP
                     EQSQMLADWYSPSHIVHGFLFYAAFASLRRKAPAERRFLVALLIEAAWEIAE"
BASE COUNT          898 a         1957 c         2025 g          949 t
ORIGIN      
        1 cgggtgaggt cccaatcagg gagcgcgatc acgccgccgc cagcctcctc agcacatagt
       61 ggaggatccc gcccgccttg aaatattcga gctcgttgta ggtgtcgatg cggcagcggg
      121 cggtgaagct gaactcctcg ccctcatcgg ggaagactcg cacctcgacg tcctgccggg
      181 ggttcaactc ggcgatgccg gcaatcgaat atttctcctt gccggtcagc cccaggctgt
      241 cggcgctgtc gccgtccttg aactgcagcg ggaggacgcc catcccgacc aggttcgacc
      301 ggtggatccg ctcgaagctc tcggcgatga ctgcgcgcac gccgagaagc actgtcccct
      361 tggccgccca gtcgcgcgag ctgccggtgc catattcctt gccggcgatc accaccagcg
      421 gggtgccgct ttccatgtag cgcatcgccg cgtcgtagat cggcagggtt tcgccggtcg
      481 gagcgtagcg cgtgaatccg ccctcgacat tgtcgagcat tcggttgcgg atgcggatgt
      541 tggcgaaggt gccgcgcatc atcacctggt ggttgccgcg ccgcgccccg tagctgttga
      601 actcggcccg cggcacgccc tgctcgacca gatatttgcc cgccgggctg tcgggcttga
      661 tcgccccggc cggcgagatg tggtcggtgg tgatcgaatc cccgaacagc gccagcgccc
      721 gggcgccggc gatgtcgccg ggcggcaccg gggtcatggt catgtcggtg aagtaaggcg
      781 ggttctggat gtaggtcgat tgcggcggcc acgaataggt ctcgccgccg ctgatctcga
      841 tcgcccgcca gcgctcgtcg ccggcgtaga cgtcgccgta gcgcgaggtg aacatgtgcg
      901 agtggaggtg ctcgtcgatc agcgcccgca cctcctcgtt gctcggccag atgtccttga
      961 ggaacaccgg ctcgccgttg gtcgcggtgc cgatcggctc gccggcgagg tcggagcgca
     1021 ccgtcccctt gagcgcgtag gcgacgacca gcggcggcga ggcgaggtaa ttggcgcggc
     1081 agtcgggcga cacccggccc tcgaagttgc ggttgcccga cagcacgctg accgcgacca
     1141 ggtcatgctc ggcgatcgcc ctggagatcg gctcggcgag cgggccggaa ttgccgatgc
     1201 aggtggtgca gccgtagccg accaggtcga accccaaggc gttgaggtcc gcgctgagcc
     1261 cgctggcgtc gagatagtcg ctgaccacct tgctgcccgg ggcgaggctg gtcttgaccc
     1321 acggcttgct ggtcagccct ttctcccgcg ccttgcgcgc caccaggccg gcggcgacca
     1381 gcaccgacgg gttggaggtg ttggtgcagc tggtgatcgc ggcgatcacc acgtcgccgt
     1441 tgccgaggtc gtaattctcg ccctcgacgg ccacccgcgg gtcgttgtgc ttcttgtagg
     1501 tggtctcgag ctcggcgttg aactggtcgt cgacgtcgct cagcagcacc cggtcctgcg
     1561 gccgccgcgg cccggcgagc gacggctcga tgctggtcat gtcgagctcg agcgtgtcgg
     1621 tgaacaatgg ctcgggcgaa ccgtcgtcgc gccacaggcc cagcgccttg caataggccc
     1681 ggaccagctc gatccgctca tcggagcggc cggtcagtct gagatattcg caggtccgct
     1741 cgtcgaccgg gaagaagccg caggtcgccc catattcggg cgccatgttg gcgatcgtcg
     1801 cccggtcggc gaggctcaat gagcccaggc ccgggccgta gaattcgacg aagcggccga
     1861 ccacgccctt ggcgcgcagc atctgggtga cggtcagcac caggtcggtc gcggtgatgc
     1921 cctcgcgaag ctcgccgtcg agccggaagc cgacgacctc ggggatcagc atgctgatcg
     1981 gctggccgag catcgccgcc tcggcctcga tcccgccgac tccccagccg agcactccca
     2041 aaccgttgac catggtcgtg tggctgtcgg tcccgaccaa cgtgtcgggg taggcgagca
     2101 tcgcccccga ctggtcctcg ctggtccaca cgcactgggc gacatattcc aaattgacct
     2161 ggtggcagat gccggtgccg ggcgggacga cctggaaatt gtcgaacgcg gaggcgcccc
     2221 atttcaagaa ctcgtagcgc tcgccgttgc gggcatattc gagctcgacg ttcttgccga
     2281 acgcgcgcgg ggtgccgaac tcgtcgacca tcacgctgtg gtcgatcacc agatggaccg
     2341 ggaccagcgg gttgatcttc tgcgggtcgc cgccgagcgc cttcatcgcg tcgcgcatcg
     2401 cggcgaggtc gacgaccgcc gggacgccgg tgaaatcctg catcagcacg cgggccggcc
     2461 ggtactggat ttcgcggctg atccgccgct ccttctgcca gtcgaccatg cactggaggt
     2521 cgtcgcgggt gacggtcgcg ccgtcctcga agcgcagcag attctcgagc agcaccttca
     2581 tcgagaatgg caggcgcgag acgtcgccga gcgcctcggc cgccttggcc agcgagaaat
     2641 aatcgtagct cttgccgccg accgtcagcg tggagcgggt gtcgaggctg tcctggccgg
     2701 tcgggatcat cgcaggtcct tgggtcgaag cgggcaacgc cgcgcaagcg ggaaatgttg
     2761 cggcgcctta cgcccgcgcg gccttacccg gcaatgctcc acagcatcgc cgccaccgcg
     2821 gcggccatcg ccgccgtggc gatccagccg aacagccgca gcgacccgac gatggtgaac
     2881 acgcccatga tcgacttgcg ctgcgccatc agcatcatca ccgccatcac cgggacggcg
     2941 acgacgccgt tgatcaccgc cgcccagtag agcgccttga tcgggtcgat gggcgtgaag
     3001 ttcatcagcg ccccgatcgc ggtcgcggcg acgatcgttc cgtagaacgc cttggcgcgc
     3061 cgcggcttgc ggtcgagccc gaccggccag cgcagcgcct cgccgagcgc gtaggcggcc
     3121 gagccggcga gcaccggcac cgcgagcagg cccgtgccga cgatccccag cgcgaacacc
     3181 gtcgatgcgt agacgccggc gaccggctcg agcgccgccg cggcctcggc cgaggtatcg
     3241 atgtcggtga ttccgttggc gttgagggtc gcggccgcgg tcaggatgat cgccagcgct
     3301 acgaggttgg acgcggccat cccggcgaac gtgtcgacct cgatgcgctt cagttcggcg
     3361 cgcccgtcct gcggcgcgca cttgagcggc tggcggccat cgatctcatg gacgtcctcg
     3421 gcctcttccg acgcctgcca gaaaaacagg taggggctga tggtcgtccc gaagatcgcg
     3481 acgatcatga tcagggccgc ttcgttgagc gcgacctcgg ggatcaccag cccgcgcagc
     3541 gcctcgctcc agggcacatc gaccaggaac aaggtgatca cataggcgag caaagcgagc
     3601 gtcagccatt tcagcacgcg gacgtaattg ccgtagcgca tgaacacttc cgacagcgcg
     3661 cagaaggcgg cgaacgcgag caggaagatc ggcgccggaa gcccggacac cagcgcggtc
     3721 gcgtcggcca tcgcgccgag gtcggcgccg atgttgatga tgttggcgac gagcagaagc
     3781 gaaaccagcg aggtcgtcag ccaggccggg taatggcggc gcaaattgcc ggcgatgccg
     3841 tgcccggtcg tccgcccgat ccgcgcgctg atcatctgca ccgacgacat caacgggtag
     3901 gtgaagatca tcgtccagcc gaggccgtag ccgaactgcg cgccggcctg gctgtaggtg
     3961 gcgatgccgc tcgggtcgtc gtccgacgcg ccggtgatca ggcccgggcc gaagatgtcg
     4021 cgaagccgga tttgcggctt cttgaaggcc ttccggcggc tgggcggttc agccgccgcg
     4081 gctcggctgg tggggtcgtt ggccatgttg aggggctgta acggcctgtg acggctccgg
     4141 ttgcgctgga atgaccagcg gctgcgccca gccgactggc cgtgactcgc cgtcgtcaca
     4201 accggtccct aggcttttcc ttgctgcccg atgaacgaag cagccatttg agggtttgga
     4261 gcgagcaatg gcccacgacc gcgaatattt ccgccgccgc gccgccgagg cgcgctccgc
     4321 cagcctccgc acggacgacg gcgagggcat cgaggtcgcc ggccacctgg cgctggctta
     4381 cgccgcgctg gcccgccgcc gcggcgctcc ggccaggccc gacgccccgg acgcggtcga
     4441 ccatgccgac gcctcggtcg ccgacgcggt cccgctgatg ctcggggact gagcgctgtc
     4501 ggctagatcc agccgcgccg cttgaaatag aggatcggca gaaccgcgct ggcgaggatc
     4561 aggaccagcg cgatcgggta gccgtacagc cagtcgagct cgggcatgtg ctcgaagttc
     4621 atcccgtaga tgcccgcgac cagggtcggc gggaggaaga tcagggccgc ccaggaaaag
     4681 agcttcatcg ccgcattctg ctcgacgctg atcagcccaa gcgccgcgtc gagcaggaag
     4741 atgaggttgt cgccgaggaa gccgctgtgg tcgattagcg aagtgacgtc ggtcatcagg
     4801 ctggccaggt ggccgcgggc gatgtcgttg gccttgatcg gctccgacgc gctgacgaag
     4861 ctcaccagcc ggccggtgct gaccgccgtc tcgcggatct tggcgagcag cgactgggcg
     4921 cggccgatgc gggtcaacag ggcggtcagc cgccgcgccg ggatccgccg ctcatccatg
     4981 tcccggccga agatctggtt ggagatcttc tcgatctccg cgcccgacgc ctcgagctcg
     5041 tcggcgagcc gctcgatgat cgcgtcgagc aggcggacca ggacggtcag ggcgtcgcgc
     5101 gccagcaccg gctcgcggag cacatgctcg ccgaacgcga gcagcggctt gggggtcgcg
     5161 tagcgcacgg tgacgaggcg gttgccggcg agaacgaagc cgatcggggt ggtcgcgggc
     5221 cggccctcgc tgactccgaa cagcgcgctc aacgtcatgt acagggcgcc gtcgcgctcg
     5281 aacagtcggc tcgacggctc gatctcggcc agctcctgct cggtcggcac attgtggccg
     5341 atcagctgct cgaccagcct ctcttcctca cgggtcggct cctcgaggtc gatccacgtc
     5401 gctcccggcg ggatcgggcc gtggtgggga tcgacgatgc tcccgtcgca atcgggcccg
     5461 taagcgcgca gcataagacg ggtgatggtt agaagcgcgg cgctttgcaa gccgcccggc
     5521 tctgctaagg ctcgagcgga tgcccactcg ctcgcgcgcc gcactggcgg cgctgctcat
     5581 cctcgccgcc accgcggccg tcctgctggc gatgggccgg ccggcgatct gcgcgtgcgg
     5641 cactgtcgag ctgtgggggg cggtcgggcc ggagcagagc cagatgctcg ccgactggta
     5701 cagcccaagc catatcgtcc acggcttcct tttctatgcg gccttcgcct cgctgcggcg
     5761 caaggcgccg gctgagcgac ggttcctggt ggcgctgctc atcgaggctg cctgggagat
     5821 tgcggagaa
//
LOCUS       DASTZO010000144        10066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_534398,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000144 DASTZO010000000
VERSION     DASTZO010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 10066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10066
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_534398"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /gene="gltA"
                     /locus_tag="VFU20_04080"
     CDS             complement(<1..895)
                     /gene="gltA"
                     /locus_tag="VFU20_04080"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HEU5285675.1"
                     /translation="MTDKTLTMTFDGGEQTYPIIPGSVGPAVVDIRKLYPDTGMFTYD
                     PGFTSTASCQSAITFIDGEKGILLHRGYPIDQLAEKSTFMEVAYLLLHGELPQKDELD
                     KFTYTISRHTMLHEQLATFYRGFRRDAHPMAVMCGVVGALSAFYHDSTDITDPLQRMI
                     ASHRLIAKMPTIAAMAYKYTIGQPFLYPQNDLSYTGNFLRMTFGVPAEPYEVNPVIER
                     AMRRIFILHADHEQNASTSTVRLAGSSGANPFACIAAGIACLWGPAHGGANEAALNML
                     REIGSVKRIPEYIARAKDKDDP"
     gene            complement(892..2301)
                     /gene="gltX"
                     /locus_tag="VFU20_04085"
     CDS             complement(892..2301)
                     /gene="gltX"
                     /locus_tag="VFU20_04085"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU5285676.1"
                     /translation="MSASHNPSPVVTRFAPSPTGFLHIGNARTGLFSWLYARHHGGKA
                     LLRIEDTDKARSTREAIDVILDGLAWLGLDWDEQVHFQSEFEARHAEVAHDLIERGAA
                     YRCYLTPEELKERREKAQAERRPFRLTSEWRDCTAAGPEGAPFVVRLKAPREGETAID
                     DLVQGRVAVANAEIDDFVLLRSDGSPTYMLAVVVDDHDMGVTHIIRGDDHLNNAFRQL
                     AIIRAMGWPEPTYAHIPLIHGPDGAKLSKRHGALGVHDYRDLGFLPEALFNYLLRLGW
                     GHGDDEIISREQAIAWFDLDHVGKSPSRFDLKKLESLNAHYIREADDGRLAELVAERL
                     GGEVDLDLLVRAMPELKARAHDLGQLADGARFLFAQRPLEMDDGAAALLTPESRALLG
                     AAHRALGKVSAWEHDELEKVVREVADEAGVKLGKLAQPLRAALTGRATSPGIFDVLAL
                     LGQGESLGRIADQMVEPNE"
     gene            2413..4260
                     /locus_tag="VFU20_04090"
     CDS             2413..4260
                     /locus_tag="VFU20_04090"
                     /inference="COORDINATES: protein motif:HMM:NF015714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HEU5285677.1"
                     /translation="MRERLEAFLERERGQLPPWFVVGFGAGIAAWFALDEPRSWAAFL
                     CVTAGLTIAGFALKGGRAERSAGWFGLAMMLGCSLVWARSHYVAAPRLERPLVTEFRA
                     EVERVETRVAKGDLRLTLALADPALPPRVQVSVKLETAPEGIAKGAQLQLRARLAPPP
                     PMALPGGYDFARDAWFRGIGAVGRSLGEVKVVEPAGETGIDAVRERLGAHVRERLPGA
                     NGGIAVALATGDQNSVLEADAEAMRRSGLTHLLSVSGLHIAAAVGAAMLLTLKLLALS
                     ERLALRFNLILVAAGVGALTGIGYTLLTGAQVPTVRSCVAALLVLAGIALGRDAISLR
                     TLAVGALLVMLFRPESLAGANFQLSFAAVASIIALHSTAWARRTFQRRDEPWVARLGR
                     AVLALIATGLAVEFTLIPIALYHFHKSGLYGVAANIVAIPLTTFVIMPLEAGALLLDI
                     VGLGPPLWWLAGLSLDALLGLAHFVAESRGAVVMMAAMPPWALGTMIAGGLWLFLWTS
                     RPRFWGLAPILVGAAGAALSPRPDLLVTGDGRHLALVDEDGTPLLLRERSGEFIRSIV
                     ADSAGFDDEPEPLESRPFASCSRDSCIAVLERGGRRWQILATRSAQSIE"
     gene            complement(4583..4927)
                     /locus_tag="VFU20_04095"
     CDS             complement(4583..4927)
                     /locus_tag="VFU20_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5285678.1"
                     /translation="MAGARIDYVELPSATAHELTRGFYSRAFGWEFTDYGPAYAATGN
                     GTVDVGLNGQPEEALAAPLPVVRVEDLEAALDAVVKAGGTIARPIFSFPGGRRFHFID
                     PSGSELAAWSDT"
     gene            complement(4930..5592)
                     /gene="lexA"
                     /locus_tag="VFU20_04100"
     CDS             complement(4930..5592)
                     /gene="lexA"
                     /locus_tag="VFU20_04100"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU5285679.1"
                     /translation="MLTAKQHELLMFINDRLGRTGVSPSFDEMREALDLKSKSGVHRL
                     ISALEERKFIRRLPNRARALEILRMPETKAPAIAAAATASSRPAVPAPANDTIEIPLH
                     GRIAAGTPIEALQGTEGFAVPAALLGPGEHYALEVSGDSMVEEGILDGDFALIRKVDT
                     ARDGEIVVALIDDSEATLKTFRHEGGMIRLDPANRQYEPQRYRPEQVRIQGRLAGLIR
                     RY"
     gene            complement(5705..7639)
                     /locus_tag="VFU20_04105"
     CDS             complement(5705..7639)
                     /locus_tag="VFU20_04105"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5285680.1"
                     /translation="MLASFRRLSKSTVGSIIMVLFVVAILASFALADISNVGSGTFGF
                     GGSSSLAEAGNRQITDRDMSRAMERRLTQVRQQNPEADYAAIAGDFDAILESLIGQAS
                     LQAFADKFGFHVSKRLVDAEIANIPGTRGLDGKFSEQAYAAFLAQQRMSDQELRQLIA
                     GGLLDRMLLTPLASNARVPVGMATPYASMLLEARQGDVANVPNSAFRAGLKPTDADLQ
                     RFYAANRARYMIPEQRVLRLARITPEQVAEIAASEQEIAAYYKANQATYGAKEIRVIS
                     QAVVPGQAAANAIAQRARGGASFAAAAAPAGLSAADISVGPQTRGEFAALAGERVAAA
                     AFAAQPGAIVGPVQSDLGWHVVKVESVRREGGKTLAAARAEIAAKLNADKRKEALTDL
                     VATVEDAIADGSNFAEAAAKARLQVTETPLITAAGTARDNPSFRLPAELAPALRSGFE
                     LTQDDEPVVETLANAEGYVLVAPSRIVPAAPAPLAAIRDRVAEDWVSKQASDRARVVA
                     SAIAAKAARGVPLAKAVAEAGVPLPPVRPLAARRLDMSQMGANVPPQMRMLFSLGAGK
                     SRIVADPQQRGFSIVKVNRIVPGNALNQPALIGRVQNEFQEAVAEEYARQFLAAIRAA
                     VGVRRNEEAIAATKARISGG"
     gene            7815..8585
                     /gene="tpiA"
                     /locus_tag="VFU20_04110"
     CDS             7815..8585
                     /gene="tpiA"
                     /locus_tag="VFU20_04110"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU5285681.1"
                     /translation="MALRKLVAGNWKMHGLAADLEQVKAIAEAARGFPSVDVALCVPA
                     TLIERAARAAPGFAIGAQDVHFAEKGAHTGCVCAAMLADAGASLAIVGHSERREAQRE
                     SDSEVRAKAQAALDAGLSVILCVGESLAVREAGEAIAAVAAQLDASLPAGLAEPVRLA
                     VAYEPIWAIGTGQVATRGDIAEMHRAVRDRLAAAYGEAGTRVRILYGGSVKPSNAAEI
                     FAVADVDGALVGGASLTAADFVPIVAAAAAVEAPPPIA"
     gene            8630..9025
                     /gene="secG"
                     /locus_tag="VFU20_04115"
     CDS             8630..9025
                     /gene="secG"
                     /locus_tag="VFU20_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU5285682.1"
                     /translation="MFTFLLIVQTVIAASLVIVILLQRSEGGGLGVGGSSSGFMTARG
                     AADFLTRSTAVLGALFVILSIVLAAVAGVSREPAKVDTSLANTVAPAPAPAAPAVPLA
                     GQAPGEAAPAAANQVAPARNDTAPVPLAQ"
     gene            9129..>10066
                     /locus_tag="VFU20_04120"
     CDS             9129..>10066
                     /locus_tag="VFU20_04120"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU5285683.1"
                     /translation="MARFIFVTGGVVSSLGKGLLSASLGALLQARGYKVRIRKFDPYL
                     NVDPGTMSPYQHGEVYVTDDGAESDLDLGHYERFTGVSARQSDNVTTGRIYRDIIARE
                     RRGDYLGATVQVIPHVTNAIKEFAIAETDGLDFVICEIGGTVGDIESLPFVEAIRQLR
                     NDIGRANGVFVHTTLVPWIAAAGELKTKPTQHSVRELTSYGIQPDVLLCRADRPIPEA
                     ERAKIALFCNVPKEAVIQALDARSIYDVPLQYHAEGLDAEVLKVFGIADAADPQLARW
                     TDIMDRVDHPEGEVTIGVVGKYVGLPDAYKSLREALV"
BASE COUNT         1384 a         3477 c         3596 g         1609 t
ORIGIN      
        1 acggatcgtc cttgtccttc gcgcgggcga tatattccgg gatccgcttc accgaaccga
       61 tttcgcgaag catgttgagc gcggcctcgt tggcgccgcc atgcgccggg ccccacagac
      121 aggcgatgcc cgcggcgatg caggcgaacg ggttggcgcc cgacgatccg gccagccgga
      181 cggtcgaggt cgacgcgttc tgctcatggt cggcgtggag gatgaagatc cgccgcatcg
      241 cccgctcgat caccgggttg acttcatagg gctcggccgg gaccccgaag gtcatgcgca
      301 ggaaattccc ggtgtagctc aagtcgttct gcggatagag gaacggctgg ccgatggtgt
      361 atttgtaggc catcgccgcg atcgtcggca tcttggcgat cagccggtgc gaggcgatca
      421 tccgctgcag cgggtcggtg atgtcggtgc tgtcgtggta gaaggccgac agcgccccga
      481 ccacgccgca catcaccgcc atcgggtggg cgtcgcgccg aaagccgcga tagaaggtcg
      541 cgagctgttc gtgcagcatg gtgtggcgcg agatggtgta ggtgaatttg tcgagctcgt
      601 ccttttgcgg cagctcgccg tggagcagca ggtaggcgac ctccatgaag gtcgatttct
      661 cggccagctg gtcgatcggg tagccgcggt ggagcaggat gcccttttcg ccgtcgatga
      721 aggtgatcgc gctctggcag ctggcggtcg aggtgaagcc cgggtcgtag gtgaacatgc
      781 cggtgtcggg gtagagcttg cggatgtcga cgaccgcggg cccgaccgag ccggggatga
      841 tcggataggt ctgctcgccg ccatcgaatg tcatcgtcag cgtcttgtcg gtcattcgtt
      901 cggctccacc atctggtcgg caatgcggcc caagctttcg ccctgcccga gcagcgcgag
      961 cacgtcaaaa attccggggc tggtggctcg gccggtcagc gccgcgcgca gcggctgggc
     1021 gagcttgccg agcttgaccc cggcttcgtc ggcgacctcg cgcaccacct tctcgagctc
     1081 gtcatgctcc caggcggaga ccttgcccag cgcccggtgg gcggcgccga gcagggcccg
     1141 gctctccggg gtcaggagcg cggcggcgcc gtcgtccatt tcgagcgggc gctgcgcgaa
     1201 caggaagcgc gcgccgtcgg cgagctggcc caggtcgtgg gcgcgcgcct tgagctcggg
     1261 catcgcccgg accagcaggt cgagatccac ctcgccgccc agccgctcgg cgaccagttc
     1321 ggccagccgc ccatcgtcgg cctcgcggat gtagtgggcg ttgaggctct cgagcttctt
     1381 caaatcgaag cgcgacggcg acttgccgac atggtcgagg tcgaaccagg cgatcgcctg
     1441 ctcgcgtgaa atgatttcgt cgtcgccgtg gccccagccg agccggagca gatagttgaa
     1501 cagcgcctcg ggcaggaatc ccaggtcgcg atagtcgtgg accccgagag cgccgtggcg
     1561 cttgctgagc ttggcgccgt cgggcccgtg gatcagcggg atgtgggcgt aggtcggctc
     1621 gggccagccc atcgcccgga tgatcgccag ctggcggaac gcgttgttga gatggtcgtc
     1681 gccgcggatg atgtgggtga cccccatgtc atggtcgtcg accaccaccg ccagcatgta
     1741 ggtcggcgag ccgtccgagc gcagcaggac gaaatcgtcg atctcggcgt tggccaccgc
     1801 gacccggccc tggacgaggt cgtcgatcgc cgtctcgcct tcgcgcggcg ctttgaggcg
     1861 aaccacgaac ggggcgcctt ccggccccgc cgccgtgcag tcgcgccact cgctggttag
     1921 tcggaacggc cgccgctcgg cctgcgcttt ctcgcgccgc tccttcaatt cctcgggcgt
     1981 cagatagcag cggtaggcgg cgccgcgctc gatcagatca tgcgcgacct cggcgtggcg
     2041 ggcctcgaac tcggactgga agtgcacctg ctcgtcccag tcgagcccga gccaggcgag
     2101 cccgtcgagg atcacgtcga tcgcctcccg ggtcgagcgc gctttgtccg tgtcctcgat
     2161 cctgagcagc gccttgccgc cgtgatggcg cgcgtacagc caggagaaca gcccggtgcg
     2221 ggcgttgccg atgtgcagga atccggtcgg ggaaggcgcg aagcgcgtga ccactggtga
     2281 cggattgtga cttgcgctca agcgctttcc tttgtcttgt cggaagcgat gcaatggatg
     2341 agcgccccta tcatagcggg tgcgcctctt ccacagcggg ctcggcggtt ctgggagacg
     2401 cgtttcggcg gcgtgcgcga gaggctcgag gcgtttctcg agcgcgagcg cgggcaattg
     2461 ccgccgtggt tcgtggtcgg cttcggcgcc ggcatcgccg cctggttcgc gctcgacgag
     2521 ccgcggtcct gggcggcgtt cctgtgcgtg acggcgggac tgacgatcgc cggctttgcc
     2581 ctgaagggcg gccgggccga gcgctccgcc ggctggttcg gcctggcgat gatgctcggc
     2641 tgctccctgg tctgggcgcg ctcgcattat gtcgccgcgc cgcgcctcga gcggccgctg
     2701 gtcaccgagt tccgggccga ggtcgagcgg gtcgagacgc gggtggccaa gggcgatttg
     2761 cggctgaccc tcgcgctcgc cgacccggcg ctgccgccgc gggtccaggt ctcggtcaag
     2821 ctggagaccg cgcccgaggg cattgccaag ggcgcgcaat tgcagcttcg cgcccggcta
     2881 gcgccgccgc cgccgatggc gcttcccggc ggctacgatt tcgcccgcga cgcctggttc
     2941 cggggcatcg gcgcggtcgg ccgctcgctc ggggaggtta aagtcgtcga gccggcgggc
     3001 gagaccggca tcgacgcggt ccgcgagcgg ctcggagccc atgtccgcga gcgcctgccc
     3061 ggcgccaatg gcggaatcgc cgtcgcgctc gcgaccggcg accagaacag cgtcctcgag
     3121 gccgacgccg aagcgatgcg gcgcagcggc ctcacccact tgctgtcggt gagcgggctg
     3181 cacatcgccg ccgcggtcgg ggccgcgatg ctcctcaccc tcaagctcct cgcgctgagc
     3241 gagcggctcg cgctgcgctt caacctgatc ctggtcgcgg ccggagtcgg ggccctgacc
     3301 gggatcggct acacgctgct gaccggcgcc caggtgccca cggtgcgcag ctgcgtcgcc
     3361 gccttgctgg tgctcgccgg gatcgcgctc ggccgcgacg cgatcagcct gcgcacgctc
     3421 gccgtcgggg cgctgctggt gatgctgttc cgccctgaat cgctcgccgg cgcaaacttc
     3481 cagctgagct tcgccgccgt cgcctcgatc atcgcgctgc actccaccgc ctgggcccgg
     3541 cggacctttc aacggcgcga cgagccgtgg gtcgcgcggc tgggccgggc ggtgttggcc
     3601 ttgatcgcca ccgggctcgc ggtggaattc acgctgatcc cgatcgcgct ctaccacttc
     3661 cacaagtccg gcctgtacgg cgtcgccgcc aacatcgtcg cgatcccgct caccactttc
     3721 gtcatcatgc cgttggaagc gggcgcgctg ttgctcgaca tcgtcggcct ggggccgccg
     3781 ctctggtggc tagccggcct gtccctggac gcgctgctcg gcctggcgca cttcgtcgcg
     3841 gagtcccgag gtgcggtcgt gatgatggcc gcgatgccgc cgtgggcgct gggaacgatg
     3901 atcgccggcg ggctgtggct gttcctgtgg accagccggc cgcgcttctg gggcctcgcg
     3961 ccgatcctgg tcggagcggc gggcgcggcc ctgtccccca ggcccgacct gctggtcacc
     4021 ggcgacgggc gccacctcgc gctggtcgac gaggacggca cgccgcttct gctgcgcgag
     4081 cggagcggcg agttcattcg cagcatcgtc gccgattccg ccggcttcga cgacgagccc
     4141 gagccgctcg agtcgcgccc gttcgcgtcc tgctcgcgcg acagctgcat cgccgtgctc
     4201 gagcgcggcg gccggcgctg gcaaatcctc gccacgcgct cggcccagtc gatcgagtga
     4261 cggccgctag tccaggcctg caacgcggcc gacatcgtcg tctcgagccg ctggctgccg
     4321 cgcggctgca cgccccgctg gctgaagctc gaccgcgatt cgctggcccg gacaggcggg
     4381 gtcgccgtct atctcggcaa ggagccgagc gccgcgaccg tcgccggtcg ggtcggcaag
     4441 cacccgtggg ccgccgatgt gcccaagcaa atgcgctacc ggccgaggat aaaggcgaag
     4501 gctggcgccg cttccacggg cgtcccggcc aacggcattt cccaggagcg ggagacgatc
     4561 gagccggcga tcgccggata gctcaggtgt cggaccaggc ggcgagttcg ctcccggacg
     4621 ggtcgatgaa gtggaatcgg cgcccgcccg ggaatgagaa gatcggcctg gcgatggtcc
     4681 cgccggcctt caccacggcg tcgagcgccg cctcgaggtc ctcgacccga acgaccggca
     4741 gcggcgccgc gagcgcctcc tccggctgcc cgttgaggcc gacgtcgacc gttccgttgc
     4801 cggtcgccgc ataagcgggc ccatagtcgg tgaactccca gccgaacgcc cggctgtaga
     4861 acccgcgcgt cagttcatgg gcggtcgcgc tcggcagctc gacatagtcg atgcgcgcgc
     4921 cggccatgct caatagcgcc ggatcagccc cgcgaggcgg ccctggatac gcacctgctc
     4981 gggccggtag cgctgcggct cgtactgccg gttcgccggg tcgaggcgga tcatgccgcc
     5041 ttcgtggcgg aaggtcttca acgtcgcctc gctgtcgtcg atcagggcga cgacgatctc
     5101 gccgtcgcgt gccgtgtcga ccttgcggat cagcgcgaag tcgccgtcga gaatgccttc
     5161 ctcgaccatc gaatcgcccg acacctcgag cgcataatgc tcgcccgggc cgagcaaggc
     5221 ggcggggacc gcgaagccct cggtgccctg cagcgcctcg atcggggtgc cggcggcgat
     5281 ccgcccgtga agcgggatct cgatcgtgtc gttggcgggg gccgggaccg ccgggcgcga
     5341 ggaagcggtc gcagccgcgg cgatcgcggg agccttcgtc tccggcatcc ggaggatttc
     5401 gagcgcccgc gcgcgattgg gcagccggcg gatgaacttg cgctcctcga gcgccgagat
     5461 cagccgatgg acccccgact tggacttgag gtcgagcgcc tcgcgcatct cgtcgaagct
     5521 cggcgacacc ccggtgcggc cgagccggtc gttgatgaac atgagcaatt catgctgctt
     5581 ggcggtgagc atcgccggtc actccccatt tgccctgcga gggaaactgg ccttagcagg
     5641 aacgaacgga gaacgtgtag gcaacgcgcc gcggcccgtc aagcgctgat tggtcggcgt
     5701 tcgcctagcc gccgctgatc cgcgccttgg tggcggcgat cgcctcttcg ttgcgccgca
     5761 ccccgaccgc agcccggatg gcggcgagga actggcgcgc atattcctcg gcgaccgcct
     5821 cctggaattc gttctggacc cggccgatca gcgccggctg gttgagcgcg ttgccgggga
     5881 cgatccggtt gactttgacg atcgagaagc cgcgctgctg cgggtcggcg acgatccggc
     5941 tcttgccggc gccgaggctg aacagcatcc gcatctgggg aggcacgttg gcgcccatct
     6001 gcgacatgtc gagccggcgc gcggcgagcg gccgcaccgg cggcaggggc acccccgcct
     6061 ccgcgaccgc cttggccagc ggcactccgc gcgccgcctt ggcggcgatt gcgctggcga
     6121 ccacccgggc ccggtccgac gcctgcttgc tcacccagtc ctcggcgacg cggtcgcgga
     6181 tggccgccag cggggcgggg gccgcgggga cgatccgcga cggagcgacc agcacatagc
     6241 cttcggcatt ggcgagcgtt tcgaccaccg gctcgtcgtc ctgggtcagc tcgaagccgc
     6301 tcctcagcgc cggcgcgagc tcggcgggca gccggaacga cgggttgtcg cgggcggtgc
     6361 cggcggcggt gatcagcggc gtctcggtga cctgcagccg ggccttggcg gcggcctcgg
     6421 cgaagttgct gccgtcggcg atcgcgtcct cgaccgtcgc cacgaggtca gtcagggcct
     6481 ccttgcgctt gtcggcattg agtttggcgg cgatctcggc tcgggccgcg gccagcgtct
     6541 tgccgccctc gcggcggacc gattcgacct tgacgacgtg ccagccgagg tcggactgga
     6601 ccggcccgac gatggcgccc ggctgcgccg cgaaggccgc cgcggcgacc cgctcgccgg
     6661 cgagcgcggc gaactcgccg cgggtctgcg gcccgaccga gatgtcggca gcgctgaggc
     6721 cggccggagc cgcggccgcg gcgaagctgg cgccgccgcg ggcgcgctgc gcgattgcat
     6781 tggcggcggc ctggccgggg accaccgcct ggctgatgac gcggatttcc ttggccccgt
     6841 aggtcgcctg gttggccttg taataagcgg cgatctcctg ctcgctggcg gcgatctcgg
     6901 cgacctgctc gggggtgatc cgggcgagcc ggagcacgcg ctgctcgggg atcatgtagc
     6961 gggcgcggtt ggcggcgtag aagcgctgca ggtcggcgtc ggtcggcttc aagccggccc
     7021 ggaacgccga gttggggacg ttggcgacgt cgccctggcg cgcctcgagc agcatcgagg
     7081 cgtacggcgt cgccatcccg accggcaccc gggcgttgct cgccaggggg gtcaggagca
     7141 tgcgatcgag caacccgccg gcgatcagct gccgaagctc ctggtcgctc atgcgctgct
     7201 gggcgaggaa tgcggcatag gcctgctcgc tgaacttgcc gtcgaggccg cgggtgcccg
     7261 ggatgttggc gatctcggcg tcgaccagcc gcttggagac gtggaaaccg aacttgtcgg
     7321 cgaacgcctg caggctggcc tgcccgatca ggctctcgag aatcgcgtcg aagtcgccgg
     7381 cgatcgccgc atagtccgcc tcggggttct gctggcggac ctgggtcagc cgccgctcca
     7441 tcgcgcgcga catgtcgcgg tcggtgatct ggcggttgcc ggcctcggcc aacgacgagg
     7501 agccgccgaa gccgaaggtc ccggaaccca cgttggagat gtcggccagg gcgaacgacg
     7561 cgaggatcgc gacgacgaac agcaccatga tgatcgaacc gaccgtcgat ttcgacaggc
     7621 ggcggaagga agcgagcatt cttgggttcc gcctcaggaa aaagttcgcg gacgctttag
     7681 gggcggcttg gcgctgcatc aaggcgagcg ggcatcaagg cgggcgggca tcaaggcggg
     7741 cgggcatcaa ggcaggcgtg ctttcgctcc gcggacaaca tggctagtgg cacgcttccg
     7801 cacagggggc atggatggcg ctgaggaagc tggttgccgg gaactggaag atgcacggcc
     7861 tcgccgccga cctcgagcag gtcaaggcga ttgccgaggc cgcgcggggt ttcccgtcgg
     7921 tcgacgtcgc tctgtgcgtg cccgcgaccc tgatcgagcg cgcggcccgc gccgcgccgg
     7981 gcttcgcgat cggcgcccag gacgtccatt tcgccgagaa gggcgcgcac accggctgcg
     8041 tctgcgcggc gatgctggcc gacgccggcg cgagcctggc catcgtcggc cattccgagc
     8101 ggcgcgaggc gcagcgcgag agcgactccg aagtcagggc caaggcgcaa gcggcgctcg
     8161 acgccggcct ctcggtgatc ctgtgcgtcg gcgaaagcct cgcggtgcgc gaagccggcg
     8221 aggcgatcgc ggccgtcgcc gcccagctcg acgcctcgct tccggccggc ctcgccgagc
     8281 ctgtgcgcct ggcggtcgcc tacgagccga tctgggcgat cggcaccggc caggtcgcga
     8341 cgcgcgggga catcgccgaa atgcaccggg cggtccgcga tcggctcgcc gccgcctatg
     8401 gcgaggccgg gacacgcgtg cgcatcctct acggcggctc ggtcaagccg tcgaacgcgg
     8461 ccgagatctt cgccgtcgcc gacgtcgacg gagcgctggt cggcggcgcg agcctcaccg
     8521 cggccgactt cgtgccgatc gtcgccgccg cggccgcggt tgaagccccg ccgccgatcg
     8581 cctagatgcg ccgcgtcact tccccgaacg tcccgagagc ctccaaagta tgttcacctt
     8641 cctgctgatc gtccagaccg tcatcgccgc ctcgctggtg atcgtgatcc tgctgcagcg
     8701 ctcggaaggt ggcgggctcg gcgtcggcgg cagctcgtcg ggcttcatga ccgcgcgcgg
     8761 cgcggcggac ttcctgaccc gctcgaccgc agtgctaggc gccctgttcg tgatcctgtc
     8821 gatcgtgctc gccgcggtcg ccggggtgtc gcgcgagccg gccaaggtcg acacctcgct
     8881 cgccaacacg gtcgccccgg cgcccgcgcc ggccgctccg gccgtgccgc tcgccgggca
     8941 ggcgccgggc gaggccgctc cggcggccgc caaccaggtt gccccggcgc ggaacgacac
     9001 ggccccggtg ccgctcgccc aataaggccg ctcatccggc gattgccgta gcttcgcgac
     9061 cactcgaaaa cttttttgcg cgcccccgct tgccctttcg ggccctagcg ccctaagcct
     9121 cgactcccat ggcgcggttc attttcgtca ccggcggcgt ggtctcatcg ctcggcaaag
     9181 gcttgctctc ggcgtccctc ggggcgctcc tgcaggcgcg cggctacaag gtccggatcc
     9241 gcaagttcga tccctatctg aacgtcgatc cggggaccat gtcgccctac cagcacggcg
     9301 aggtctatgt gaccgacgat ggggcggaga gcgacctcga cctcggccat tacgagcgct
     9361 tcaccggggt ctcggcgcgc cagtcggaca atgtcaccac cggccgcatc taccgcgaca
     9421 tcatcgcccg cgagcgccgc ggcgactatc tcggagcgac cgtccaggtg atcccccacg
     9481 tcaccaacgc gatcaaggag ttcgcgatcg ccgagaccga cggcctcgat ttcgtcatct
     9541 gcgagatcgg cggcaccgtc ggcgacatcg agagcctgcc gttcgtcgag gcgatccgcc
     9601 agctcaggaa cgacatcggc cgcgccaacg gagtgttcgt gcacaccacg ctggtgccgt
     9661 ggatcgcggc ggccggcgaa ttgaagacca agccgaccca gcattcggtg cgcgagctga
     9721 ccagctacgg aatccagccc gacgtgctgc tgtgccgcgc cgaccggccg atcccggagg
     9781 cggagcgcgc caagatcgcg ctgttctgca acgttcccaa ggaagccgtc atccaggcgc
     9841 tggacgcgcg gtcgatctat gacgtgccgc tccaatatca tgccgaaggc ctcgatgcgg
     9901 aagtgttgaa ggtgttcgga atcgccgacg ctgcggatcc ccagcttgcc cgctggaccg
     9961 acattatgga ccgggtcgac catccggagg gagaggtcac cattggcgtc gtcggcaaat
    10021 atgtcggact gccggacgcc tacaagtcgc tccgcgaggc gctggt
//
LOCUS       DASTZO010000145         5735 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_299525,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000145 DASTZO010000000
VERSION     DASTZO010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5735
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299525"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /gene="lptE"
                     /locus_tag="VFU20_04125"
     CDS             <1..410
                     /gene="lptE"
                     /locus_tag="VFU20_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HEU5285684.1"
                     /translation="AATLRSVEVAPIDGQAGWLVHHKLKERLGAAGEGAPAYRLEVEL
                     DDNITGFGIRGDRAVTRERRTLRARYRLVDLTGGGVVLDATAGSDAGIDVVSSEYATI
                     AAEQTALERLSEVVADQIVARLGLFAARTAAPR"
     gene            418..1440
                     /locus_tag="VFU20_04130"
     CDS             418..1440
                     /locus_tag="VFU20_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU5285685.1"
                     /translation="MKAAKGSVGRALDHPDGDVRMYLLHGPDEGQSRALGERLLKGLG
                     ATRFVVAAAAVKSDPALLADEAGAMNLFGEPRAIWVEPAGDEIAEGVEALLEAPASES
                     PVIVIAGALRKTSALLKLAEGHRSALAHASYIPEGQDAERMVAEVGRAHGLRIAPGVA
                     ARVADACGNDQAIVGQELAKLALYVGASPEAPKELDHDALDAVGADAPEGDFLRLADL
                     ALSGELAEVADELARLSPGGSEAIPVIRALQRRLLMLAPMRARVEGGERSSAVMTSLG
                     KSLFWKDKPLLEKLLATWDARGLETVAERAGRLERQLMRADSPPPAEALGEELAAIAR
                     RAARRR"
     gene            complement(1445..2326)
                     /locus_tag="VFU20_04135"
     CDS             complement(1445..2326)
                     /locus_tag="VFU20_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU5285686.1"
                     /translation="MNGKRPVPGLGRGLSALIDESARPAVEAEGAGDSRGGVREIEVG
                     RIRPNPNQPRMHFSEESIDELADSIGERGVLQPILLRPHGDGYEIIAGERRWRAAQRA
                     RLHTIPAIVRDVDDAAAAEIALIENIQREDLNAIEEAEGYRQLIDNHGHSAEDIGRLV
                     HKSRSHVANLLRLLDLPVFVRDALVRGDISMGPARAVAAAPDPEALAKEIIAKGLSVR
                     QAESRARREKKPPGELQLTERRSDPDLEALERQLGDLLGVRVRVAHNGAAGTVTLHYN
                     SLDQLDMICQRLTGEPI"
     gene            complement(2323..3102)
                     /locus_tag="VFU20_04140"
     CDS             complement(2323..3102)
                     /locus_tag="VFU20_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006966449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEU5285687.1"
                     /translation="MIRVAIANQKGGVGKTTTAINLATALAAIGWKVLLVDLDPQGNA
                     STGLGVGQAQRRRSSYDVLIGNSSVADSVVASRVPRLDLLPSTVDLSGAEIELVALDD
                     RAHRLERALVAADARWDICLIDCPPSLGLLTVNALVAARQLLVPLQCEFFALEGLSQL
                     LKTVERIRQAFNPELAILGVVLTMFDRRNNLSQAVADDVRACLGPAVFETMIPRNVRL
                     SEAPSHGLPALIYDLRCAGSEAYVRLARELMARLPQRAEAA"
     gene            complement(3099..3719)
                     /gene="rsmG"
                     /locus_tag="VFU20_04145"
     CDS             complement(3099..3719)
                     /gene="rsmG"
                     /locus_tag="VFU20_04145"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEU5285688.1"
                     /translation="MIDALERAAGRPVSRETADLLETYVERLKSANSTQNLVAASTLG
                     AIWQRHILDGAQLVRFEPHPDASWVDIGSGAGLPGMVVAALVEGPVALVEPRRLRAAF
                     LEETAAALGLADRVSVRASKIEHVHGSFDVITARAVAPLTRLLAMAHHLAHSETIWAL
                     PKGKSADSELAEANRSWQCEARREASCTDPQATILVLSKVKAKSKR"
     gene            complement(3716..5395)
                     /gene="mnmG"
                     /locus_tag="VFU20_04150"
     CDS             complement(3716..5395)
                     /gene="mnmG"
                     /locus_tag="VFU20_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEU5285689.1"
                     /translation="MFDVLVIGAGHAGCEAAAAAARRGARVGLLTFRREDLGQMSCNP
                     SIGGVGKGHLVRELDVFDGLMARAADRSAIHRRMLNRSKGPAVHGPRVQADRGLYRAA
                     IAELVAESGVEAVVAEALRLRIEHGRVTGVETSAGTFACRALVIATGTFLDARIFIGE
                     EILPGGRRGERASVALGRQLREIGLGQGRLKTGTPPRIDGRTIDWARLDEQPSDADAW
                     TMSALDDGVRRPQLACAIARTNARTHDIIRSAFDRSPLFAGAIEGRGPRYCPSIEDKV
                     KRFGERDGHQIFLEPEGLADALVYPNGISTSLPADVQRAFVRSIAGLERAELVRPGYA
                     VEYEYVDPRRLNSTLQVRGLAGLFLAGQINGTTGYEEAAAQGLAAGLNAAANALGLAE
                     VRFDRRTSYVGVMIDDLTLQGVSEPYRMMTARAEHRLSLRADNATSRLGEAALAAGCV
                     SNRRRAQIEAHMKLRGTTGWAATDEGRADALYAPYVERQRREWDAVRRDCAVWIPADM
                     DYAAVPGLSAEMVERLSASRPETLDQASRVHGVTPAALSAVYVAASRRAAA"
     gene            complement(5461..>5735)
                     /locus_tag="VFU20_04155"
     CDS             complement(5461..>5735)
                     /locus_tag="VFU20_04155"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU5285690.1"
                     /translation="TGEGIAPLLERVAELAKSLLPGEDSLALNRRQAAHLEEAREALA
                     RAGAAADIVTVAEDLRLARGAFDRLTGRAGVEDVLDALFARFCLGK"
BASE COUNT          812 a         2035 c         2066 g          822 t
ORIGIN      
        1 tcgcggcgac cctgcgctcg gtcgaggtgg cgccgatcga cggccaggcc ggctggctgg
       61 tccaccacaa gttgaaggag cgcctcgggg ccgccggcga gggcgcgccc gcctatcggc
      121 ttgaggtcga gctcgacgac aatatcaccg gattcgggat ccgcggcgac cgcgcggtga
      181 cccgcgagcg gcgcacgctc cgggcgcgct accggctggt cgacctgacc ggcggcggag
      241 tggtgctcga cgcgactgcg ggctccgacg cgggaatcga cgtcgtcagc tcggaatatg
      301 cgaccatcgc cgccgagcag accgcgctcg agcgcctgtc cgaggtcgtc gccgaccaga
      361 tcgtcgcccg cctcggactg ttcgccgccc ggacggccgc gccccggtga ggcggtggtg
      421 aaagccgcca agggcagcgt cgggcgggcg ctcgaccacc ccgacggcga cgtccggatg
      481 tatttgctcc acggccccga cgagggccag tcgcgggcgc tcggcgagcg attgctcaag
      541 ggcctgggcg cgacccgctt cgtggtcgcc gcggccgcgg tcaagtcgga cccggcgctg
      601 ctcgccgacg aagccggggc gatgaacctg ttcggggagc cgcgcgcgat ctgggtcgag
      661 ccggccggcg acgagatcgc cgagggggtc gaggcgctgc tcgaggcgcc ggcgtcggaa
      721 agcccggtga tcgtcatcgc cggggcgctg cgcaagacgt cggcgctgct caagctggcc
      781 gagggccatc gctcggcgct cgctcacgcc tcctacatcc ccgagggcca ggatgccgag
      841 cggatggtcg ccgaggtcgg gcgggcgcac ggcctgcgga tcgctccggg ggtcgcggcg
      901 cgggtcgccg acgcgtgcgg caacgaccag gcgatcgtcg gccaggaact cgccaagctc
      961 gctttgtacg tcggagcgtc gccggaagcg cccaaggaac tcgaccatga cgcgctcgac
     1021 gcggtcggcg ccgacgcgcc cgagggcgat ttcctgcgat tggccgacct tgccctctcg
     1081 ggcgagctgg ccgaagtcgc cgacgagctg gcgcggctgt cgccgggcgg gagcgaggcc
     1141 atcccggtga tccgcgcgct tcagcggcgg ctgctgatgc tggctccgat gcgcgcccgg
     1201 gtcgagggcg gcgagcgctc gtccgccgtc atgacgtcac tcggcaagtc cttgttctgg
     1261 aaagataaac cgctgctcga gaagctgttg gcgacgtggg acgcgcgcgg cctggagacg
     1321 gtcgccgagc gcgccggacg gctcgaacgg cagctgatgc gcgccgattc gccgccgccg
     1381 gccgaggctc tcggcgagga gctggcggcg atcgcgcgcc gggccgcgcg gcggcgctaa
     1441 aaggctagat cggctcgccc gtcagccgct ggcagatcat gtcgagctgg tcgaggctgt
     1501 tgtagtgcag cgtcaccgtg ccggcggcgc cgttgtgagc gaccctgacc cggaccccga
     1561 gcaggtcgcc cagctggcgc tcgagcgcct cgaggtcggg gtcgctgcgg cgctcggtca
     1621 gctgcagctc gcccggcggc ttcttctcgc ggcgagcgcg cgattcggcc tgtcggaccg
     1681 acagcccctt ggcaatgatc tccttggcca gcgcctcggg gtcgggcgcg gcggcgacgg
     1741 cgcgcgcggg gcccatgctg atgtcgccgc ggaccagcgc gtcgcggacg aacaccggca
     1801 ggtcgagcag gcgcagcagg ttggcgacgt ggctgcgcga cttgtggacc agccggccga
     1861 tgtcctcggc gctgtggccg tggttgtcga tcagctgccg gtagccctcg gcctcctcga
     1921 tcgcgttcaa atcctcgcgc tggatattct cgatcagggc gatctcggcc gcggccgcgt
     1981 cgtcgacgtc gcggacgatc gccgggatgg tgtgcagccg cgcgcgctgc gccgcccgcc
     2041 agcggcgctc gccggcgatg atctcgtagc cgtcgccgtg cgggcgcagc aggatcggct
     2101 gcaggactcc gcgctcgccg atcgaatcgg ccagctcgtc gatcgattcc tcggaaaaat
     2161 gcatgcgcgg ctggttgggg ttggggcgga tccggccgac ctcgatctcg cgcactccgc
     2221 cgcgcgagtc gcccgcgccc tcggcctcga cggccgggcg cgccgattca tcgatcagcg
     2281 ccgacaggcc gcggccgagc cccggcacgg gccgcttgcc gttcatgccg cctcggcgcg
     2341 ctgcggaagc cgcgccatca gctcgcgcgc caggcggaca taggcttccg agcccgcgca
     2401 ccggaggtca tagatcagcg ccgggagccc atggctcgga gcctcggaca gccggacgtt
     2461 gcgcgggatc atcgtctcga acaccgccgg gccgaggcac gcccggacgt cgtcggcgac
     2521 ggcctgcgaa agattgttac ggcggtcaaa cattgtcagc acgacgccga ggatcgcgag
     2581 ctccgggttg aacgcctggc ggatccgctc gacggtcttc agcagctggc taagtccttc
     2641 caatgcaaag aattcgcact gcagcgggac cagtagctgg cgcgccgcga cgagcgcgtt
     2701 gacggtcagc aggccgagcg acggcgggca gtcgatcagg cagatgtccc agcgcgcgtc
     2761 ggccgccacg agcgcccgct cgagccggtg ggcccggtcg tcgagcgcga ccagctcgat
     2821 ctcggccccc gacaggtcga cggtcgacgg cagcaggtcg agccgcggca cccggctggc
     2881 gacgacgctg tcggcgacgc tcgaattgcc gatcagcacg tcatagctcg agcgccggcg
     2941 ctgcgcctgg ccgactccga gcccggtcga ggcgttgccc tgcgggtcga ggtcgaccag
     3001 cagcaccttc cagccgatcg cggcgagcgc ggtggcgagg ttgatcgcgg tggtcgtctt
     3061 gcccaccccg cccttctggt tggcgatcgc tacccggatc atcgtttcga cttcgccttc
     3121 actttgctca gcaccaggat cgtcgcttgc gggtcggtgc agctcgcttc gcgtcgcgcc
     3181 tcacattgcc agcttcgatt cgcttcggcc agttccgaat cggcgctttt gcccttggga
     3241 agcgcccaaa tcgtctccga atgcgccaga tggtgcgcca tcgccagcaa tcgggtgagc
     3301 ggagcgaccg cgcgggcggt gatcacgtcg aaactgccat ggacatgctc gatcttcgat
     3361 gcccggacgg agacgcgatc ggccaggccg agcgcggcgg ccgtctcctc gaggaacgcc
     3421 gcgcgaagcc ggcgcggctc gaccagggcg accggcccct cgaccagcgc cgcgacgacc
     3481 atccccggca ggcccgcgcc cgagccgatg tcgacccacg acgcgtcggg atgcggctcg
     3541 aagcggacca gctgggcgcc gtcgaggatg tgccgctgcc agatcgcgcc gagggtcgag
     3601 gcggcgacca gattctgcgt ggaattggcg gatttcagcc gttccacata ggtttcgagc
     3661 aggtcggctg tttcacgtga aacagggcgg cccgccgccc gttccagcgc atcgatcatg
     3721 cggccgcgcg gcgagaggcg gcgacataga ccgcggacaa agccgccggc gtgacgccgt
     3781 ggacccgcga tgcctggtcg agggtctcgg gccgcgacgc gctcagccgc tcgaccatct
     3841 cggccgagag gcccgggacc gcggcataat ccatgtcggc cgggatccac accgcgcaat
     3901 cgcggcggac cgcgtcccat tcgcgccgct ggcgctcgac ataaggggca tatagcgcgt
     3961 cggcgcggcc ctcgtcggtc gcggcccagc cagtcgtgcc gcgcaacttc atatgggcct
     4021 cgatctgggc gcggcgccgg ttcgaaacgc agccggcggc gagcgccgcc tcgccgagcc
     4081 ggctggtcgc attgtcggcg cgcagcgaca gccggtgttc ggcgcgcgcg gtcatcatcc
     4141 ggtagggctc gctgactccc tgcagagtca ggtcgtcgat catcaccccg acgtagctgg
     4201 tccgccggtc gaagcggact tcggccagcc cgagcgcgtt tgcggcggcg ttgaggccgg
     4261 ccgccagccc ctgcgcggcg gcttcctcat agccggtggt gccgttgatc tgcccggcga
     4321 ggaacagccc ggccaggccg cggacctgga gcgtcgaatt gagccggcgc gggtcgacat
     4381 actcatattc gaccgcgtag ccggggcgga cgagctcggc gcgctcgagc ccggcgatgc
     4441 tccgaacgaa cgcgcgctgg acgtcggcgg gaagcgaagt cgaaattccg ttgggataga
     4501 cgagagcgtc ggcaagccct tccggctcaa ggaaaatctg gtgcccgtcg cgctcgccga
     4561 agcgcttcac cttgtcctcg atcgacgggc agtagcgcgg cccgcggccc tcgatcgcgc
     4621 ccgcgaacaa gggcgagcgg tcgaacgccg agcggatgat gtcgtgggtg cgcgcgttgg
     4681 tccgcgcgat cgcgcaggcg agctgcggcc ggcggactcc gtcgtcgagc gccgacatgg
     4741 tccatgcgtc ggcgtcgctc ggctgctcgt cgagccgcgc ccagtcgatc gtccggccgt
     4801 cgattcgcgg cggggtgccg gtcttgagcc ggccctggcc gaggccgatc tcgcgcagct
     4861 gccggccgag cgccaccgac gctctctcgc cgcggcggcc gcccggaagg atctcctcgc
     4921 cgatgaagat ccgggcgtcg aggaaggtgc cggtcgcgat caccagcgcc cgacaggcaa
     4981 aggtgccggc gctggtctca acccccgtga cccggccatg ctcgatccgc aggcgcagcg
     5041 cctccgcgac gacggcctcg actccgctct ccgcgaccag ctcggcgatc gccgcccggt
     5101 acaggccgcg gtcggcctgg acgcgcgggc cgtggacggc cgggcccttg cttcggttga
     5161 gcatccggcg gtggatcgcc gagcggtcgg cggcgcgggc catcagtccg tcgaacacgt
     5221 cgagctcgcg gaccagatgg cccttgccga ctccgccgat cgacgggttg cacgacatct
     5281 ggccgagatc ctcgcgccgg aaggtcagca ggccgacacg cgcgccgcgc cgcgccgccg
     5341 cggccgcggc ttcgcatccg gcatggccgg cgccgatcac gagcacatcg aacatgcgcc
     5401 cgctctacgg aaaatcacgc ttctgcacaa atgcagaacc cgtgtttcac gtgaaacaat
     5461 tcactttccg aggcagaagc gcgcaaacag cgcgtcgagc acgtcctcca cgccggctcg
     5521 gcccgtcaga cgatcgaacg caccgcgcgc cagccgcaga tcttccgcaa ccgtaacgat
     5581 atcggccgcc gcgcccgccc gggccagtgc ctctcgcgct tcctccagat gagcggcctg
     5641 gcgccggttc agggccagcg agtcctcgcc cggcagaagg gacttggcca gctcggcgac
     5701 cctctcgagc aatggggcga tgccctcgcc ggtca
//
LOCUS       DASTZO010000146         2199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1210824,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000146 DASTZO010000000
VERSION     DASTZO010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1210824"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..974)
                     /locus_tag="VFU20_04160"
     CDS             complement(225..974)
                     /locus_tag="VFU20_04160"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEU5285691.1"
                     /translation="MSSHTPDARAAEDMLRRRYAWTDVEEAEILVALGGDGFMLQTLH
                     DMLEGGSARPVFGMNRGTVGFLMNEWRIDQLAERIGSAKAIRVAPLEMVATTVGGEVH
                     THAAINEVSLLRETRQTAKIEVLVNGRVAMPELACDGVLVATPAGSTAYNLSARGPIL
                     PLQAKMLALTPISPFRPRRWSGAILPDDTAVCFRVLEPENRPVSAVADQFEVRDVARV
                     DVRLDRERSLTLLFDPEHALDDRIAMEQFTT"
     gene            1179..2012
                     /locus_tag="VFU20_04165"
     CDS             1179..2012
                     /locus_tag="VFU20_04165"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5285692.1"
                     /translation="MFKWFSGYSGGERASDRQGPAPAVPKRDRILEAVIAHQQIALLF
                     QPQIDPSSGDIVGVEALARWDGAGSPEELFVRAAASGLAERLSRLVQRKALRLAATWE
                     GPLKGLNLSINLLPQDLARPGYDQWLLDEIGAAGIDPKRITVEITESALLSDSDSIAE
                     RLARLRSAGVRVAVDDFGTGYASLAYLTSLPLDILKIDRGLIADIVGGARDRIVVKAM
                     IALARELDLKVVVEGVESTAQLALLAEWGCDLYQGFLGAGPLDEVELARFVAASMAEA
                     A"
     gene            complement(2009..>2199)
                     /locus_tag="VFU20_04170"
     CDS             complement(2009..>2199)
                     /locus_tag="VFU20_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285693.1"
                     /translation="PDLGGLLGYIERLKGSAKLRPDSKLSVARDWPSAEARLGGALQL
                     SRGLARVAAAGEAARKAA"
BASE COUNT          326 a          776 c          770 g          327 t
ORIGIN      
        1 gattcgaaca ggcgaccatt cgattaacag tcgaacgctc taccgctgag ctaccgggga
       61 acacctctcg caaaggcgag gcggctaaat agcggagcgg cccccaaaaa caagggcgcc
      121 tttgatgagc actgcacctg cccacacccc cacccggccg cccattcagt gtacgctcgt
      181 gggtggccgg gtgggggtgc gggccggcgc agtgcattcc aaaatcaggt cgtgaactgc
      241 tccatggcga tgcggtcgtc gagcgcgtgc tcggggtcga acagaagcgt cagcgagcgc
      301 tcgcggtcga ggcggacatc gacccgggcg acgtcgcgca cctcgaactg gtcggcgacc
      361 gccgagaccg gccggttctc gggctccagg acccggaagc agaccgcggt gtcgtcgggc
      421 aggatcgcgc ccgaccagcg gcgcggccga aacgggctga tcggggtcag cgccagcatc
      481 ttggcctgca gcggcaggat cggcccgcgc gccgaaagat tgtaggcggt ggagccagcc
      541 ggggtcgcga ccagcacccc gtcgcaggcc agctccggca ttgccacgcg gccgttgacc
      601 agcacctcga tcttggccgt ctggcgggtc tcgcgcagca gcgacacttc gttgatcgcg
      661 gcgtgggtgt ggacctcgcc gccgaccgtc gtcgcgacca tctccagcgg cgcgacccgg
      721 atcgccttgg cgctcccgat ccgctcggcg agctggtcga tccgccactc gttcatcagg
      781 aagccgacgg tgccgcggtt catcccgaac accgggcgcg ctgagccgcc ctcgagcatg
      841 tcgtggagcg tctggagcat gaagccgtcg ccgcccagcg cgaccaggat ctcggcctcc
      901 tcgacgtcgg tccacgcata gcggcggcgc agcatgtctt cggcggccct ggcgtccggg
      961 gtgtgcgagg acatcagccc gatgccgagg ctcgcgaggt cctgccggac gccttcatcg
     1021 gcgaggttgg gcgcgtcggc gctcaatcgc gggccctcct ggaaatcgcc gtcatgcgcg
     1081 gaacataggg attttggccg gaggcgtcac tgtcccgttg tggggcgggc ggcattggcc
     1141 gcttccggtc gtgcccgcgg cgcgttagca gacggctcat gttcaaatgg ttcagcgggt
     1201 acagcggggg ggagcgggcg tcggatcggc aaggtccggc gcccgcagtc ccgaagcgcg
     1261 accggatcct cgaagcggtc atcgcccacc agcagattgc gctgctgttc cagccgcaga
     1321 tcgacccttc gagcggcgac atcgtcgggg tcgaggcgct ggcgcgctgg gacggcgcgg
     1381 gctccccgga agagctgttc gtgcgcgccg ccgcaagcgg cctcgccgag cgcctgtcgc
     1441 ggctggtcca gcgcaaggcg ctgcgcctcg cggcgacctg ggaagggccg ctcaaggggc
     1501 tcaacctgtc gatcaacctg ctgccgcagg atctcgcccg cccgggttac gaccagtggc
     1561 tgctcgacga gatcggcgcc gccggcatcg acccgaagcg gatcaccgtc gagatcaccg
     1621 aaagcgcgct cctgtccgac agcgattcga tcgccgagcg cctggcccgg ctgcgcagcg
     1681 cgggggtccg cgtcgcggtc gacgatttcg gcaccggcta cgcaagcctc gcctacctca
     1741 ccagcctgcc gctcgacatc ttgaagatcg accgcggcct catcgccgac atcgtcggcg
     1801 gcgcgcgcga ccggatcgtc gtcaaggcga tgatcgcgct cgcccgcgag ctcgatttga
     1861 aggtggtggt cgagggcgtc gagagcaccg cccagctcgc gctgctcgcc gaatggggct
     1921 gcgacctcta ccaggggttc ctcggcgccg ggccgctcga cgaggtcgag ctcgcccgct
     1981 tcgtcgccgc ctcgatggcc gaagccgcct aggccgcttt cctcgcggct tcgccggccg
     2041 cggcgactcg cgccagcccc cgcgacagct gcagcgctcc gccgagcctg gcctcggcgc
     2101 tcggccagtc gcgggcgacc gacagcttgc tgtcgggccg aagcttggcc gagcccttca
     2161 accgctcgat atagccgagc agcccgccga ggtcgggga
//
LOCUS       DASTZO010000147         5058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_142902,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000147 DASTZO010000000
VERSION     DASTZO010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5058
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142902"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VFU20_04175"
     CDS             complement(<1..1014)
                     /locus_tag="VFU20_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HEU5285694.1"
                     /translation="MNQILLGLAGILVILALAFALSTNRKAIRLRVVAAAFALQAGIA
                     VLVLYTPWGIAAIKGMSNGVANLLGYANEGTEFLFGPSEKNPLALTFALGALPVIIFF
                     ASIVAILYYLGIMQRVVRWVGGAIGWVTGISRVESLSAAANIFVGQSESPLVIRPYLA
                     ALPPSRLFTVMTVGMAGVAGTILAAYASLLGERYLPYLLAAAFMSAPGGILMAKIIMP
                     DDPPGPDELPLEGGVVDEDQVDVAETFEEGERPANIIMAAAQGAQTGVKLAVAVGAMV
                     LAFVALVALANGLLGGLGNMVGVPDLSFQRLVGYIFAPIMFLIGVPWAEAGTAGGLFG
                     TKLV"
     gene            complement(1045..1623)
                     /locus_tag="VFU20_04180"
     CDS             complement(1045..1623)
                     /locus_tag="VFU20_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU5285695.1"
                     /translation="MKNIRRLAVYCGSSIGSDPMFADAAVELAQVMAGRGVDLVYGGG
                     RLGLMGLIADSVLAAGGRAYGVIPNALVDLEVAHTGLTELYRVDTLHERKAKMTDLAD
                     AFVALPGGIGTLDELFEAWSWNALGYHAKPLALLNVAGFWDGLDSFLDHAAACGFLSP
                     ARREQLLVAESADEALKLLDEAAATAAKGMVW"
     gene            complement(1620..2606)
                     /locus_tag="VFU20_04185"
     CDS             complement(1620..2606)
                     /locus_tag="VFU20_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HEU5285696.1"
                     /translation="MTSPKLDIVAIGDAIVDVIATCDDAFLAERGLQKGSMRLLDSDE
                     ADDLYAAMRPARETSGGSAANSMAGVAALGLDAGFIGQVADDQLGEIFAHDMRSLGVR
                     FDTPPLSNGPPTGRCLILVTPDAQRTMNTCPGASHELTPQALDEELVRSAAITFLEGY
                     LWGPERPRAAMLQAAEIAHQADRAVAFTLSESLCIPGRREGVQAMIDSGAIDLLFANE
                     DEVRHLTGSDDLERAIGQLSAKIATLVVTRGAAGAMALEDGQRIEIPAAPVERVVDTT
                     GAGDLFAAGFLAARCRGHDLRRCLDAGALAAAEIISHFGARPEADLKQLVRL"
     gene            2799..3377
                     /locus_tag="VFU20_04190"
     CDS             2799..3377
                     /locus_tag="VFU20_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HEU5285697.1"
                     /translation="MVCIAGVLGNKQVALGPLAVEAGIFAFLFLVITSSAVAELHGRS
                     IANRLVLIGFVPLIASLLLTLLVLGTPAADDMDPRRLEAFETVMGGTPRIWLGGILAY
                     GTSQILNVTIFSRLKRSGGRLLWLRSAVASVLSQIVDTLIFITVAFYGVFPIAELLMG
                     QMLAKVVLSLIFVPPLVYLFVALGRRLDERRA"
     gene            3625..>5058
                     /locus_tag="VFU20_04195"
     CDS             3625..>5058
                     /locus_tag="VFU20_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007704872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEU5285698.1"
                     /translation="MPAAAHAQSTGSVDFEKDAIIVTGTRVRDVGGVQAPDAAKAKAV
                     LTQEIIARQNPGQTILDTINLVPGVSFQNNDAYGSSGGNLTIRGFDSTRISLTFDGIQ
                     LNDSGNYAIYSNQQLDPELIEQVNVNLGSTDVDSPTASAVGGTVNYRTMIPASDMNLR
                     GLISLGEWDFRRFFAVWNIGTVGPWGTRAFVSASKADNRVPFNGYGKIDKQQYNARIW
                     QPVGAAGDFVSVSGHYNQNRNNFFGSLPLRLDTTQSTTNSAPRVVGSGTSNRYPGSRD
                     ERFYDINDTCVLDTPQAGVADAPAPAPPNQASCGTEFDRRYNPSNTGNIRGASRFTIA
                     EGLVLTVDPSYQYVKANGGGTVTGEEGLRDINPAGGTASVASCRTTPISTNNRCVAGY
                     LGGTPFFGRDINGDGDVLDRVTVLAPSQTQTHRYGVIAGLRWELAPSHTVRVSYTLDH
                     ARHRQTGEVGMLEIDGEPEDVFPINDPE"
BASE COUNT          840 a         1718 c         1708 g          792 t
ORIGIN      
        1 gaccagcttg gtgccgaaca gtccgccggc ggtgccggcc tcggcccacg gcaccccgat
       61 caggaacatg atcggcgcga agatgtagcc gaccagccgc tggaagctga ggtcggggac
      121 tccgaccatg ttgccgaggc cgccgagcag cccgttggcg agcgcgacca aagcgacgaa
      181 cgccagcacc atcgcgccga ccgcgacggc gagcttgacc ccggtctggg cgccctgggc
      241 cgcggccatg atgatgttcg ccggccgctc gccctcctcg aacgtctcgg cgacgtcgac
      301 ctggtcctca tcgaccacgc cgccctccag cggcagctcg tccggccccg gcgggtcgtc
      361 gggcatgatg atcttggcca tcaatatgcc gcccggcgcc gacatgaagg ccgcggcgag
      421 caggtaaggc agatagcgct cgccgagcag gctcgcgtag gcggcgagga tggtcccggc
      481 gaccccggcc atgccgacgg tcatcacggt gaacaggcgc gagggcggca gtgcggcgag
      541 ataggggcgg atgaccaaag gcgattcgga ctggccgacg aagatgttgg cagcggcgct
      601 cagcgattcg acgcggctga ttccggtgac ccagccgatc gcaccgccga cccagcgcac
      661 gacccgctgc atgatgccga ggtaatagag gatggcgacg atcgaggcga agaagatgat
      721 caccggcagc gcgcccaggg caaaggtcag cgccagcgga ttcttttccg acgggccgaa
      781 caggaactcg gtgccctcgt tggcgtagcc gagcagattg gcgaccccgt tggacatgcc
      841 cttgatcgcg gcgatccccc acggcgtgta gagcaccagc accgcgatcc ccgcctgcag
      901 cgcgaaggcg gcggcgacga cccgcaggcg gatcgccttg cggttggtcg acaaggcgaa
      961 cgccaaagcg aggatgacaa ggatgccggc gagcccgagc aggatttggt tcattgaatt
     1021 ccccccaggg ctacgcgacg cgccttacca gaccatgcct tttgcagcgg tcgcggcggc
     1081 ttcgtccagc agtttgagcg cctcgtccgc actctcggcg accagcagct gctcgcgccg
     1141 cgccggcgac aggaagccgc acgcggcggc atggtcgagg aacgaatcga ggccgtccca
     1201 gaagcccgcg acgttgagca aggcgagcgg cttggcgtgg tagccgagcg cgttccagga
     1261 ccaggcctcg aacagttcgt cgagcgtgcc gatgccgccg ggcagagcga cgaacgcgtc
     1321 ggcgagatcg gtcatcttcg ccttgcgctc gtggagcgtg tcgacgcggt acagctcggt
     1381 caggccggtg tgcgcgacct cgaggtcgac cagcgcgttg ggaatgaccc cgtaggcgcg
     1441 gccgccggcg gcgagcacgc tgtcggcgat cagccccatc agcccgagcc ggccgccgcc
     1501 gtagaccagg tcgacaccgc gcccggccat gacctgggca agctcgaccg cggcgtcggc
     1561 gaacatcggg tcggagccga tcgacgatcc gcagtagacg gcgaggcggc ggatgttctt
     1621 cacagccgca ccaattgctt gaggtcggct tcggggcggg cgccgaaatg ggagatgatc
     1681 tccgcggcgg cgagggcgcc ggcgtcgagg cagcgccgga gatcgtggcc gcggcagcgg
     1741 gcggcgagga agccggcggc gaacaggtcg ccggcgccgg tcgtgtcgac cacccgctcc
     1801 accggcgcgg cggggatttc gatccgctgg ccatcctcga gcgccattgc ccccgccgca
     1861 ccgcgggtga cgaccagggt cgcgatcttc gccgacagct gcccgatcgc ccgttcgagg
     1921 tcgtcgctgc cggtcaggtg ccggacctcg tcctcgttgg cgaacaacag gtcgatcgcg
     1981 ccggaatcga tcatcgcctg gacgccttcg cgccggcccg ggatgcacag gctttccgac
     2041 agggtgaagg cgaccgcgcg gtcggcctga tgggcgatct cggccgcctg cagcatcgcc
     2101 gcgcgcgggc gctcggggcc ccacagatag ccttcgagga aggtgattgc ggccgagcgg
     2161 accagctcct cgtcgagcgc ctgcggagtg agctcgtggc tggcgccggg gcaggtgttc
     2221 atggtccgct gggcgtcggg cgtgacgagg atcaggcagc ggccggtcgg cggtccgtta
     2281 ctgagcggcg gagtgtcgaa ccgaaccccg agcgagcgca tgtcgtgggc gaaaatctcg
     2341 ccgagctggt cgtcggcgac ctggccgatg aagccggcgt cgaggccgag cgcggcgacc
     2401 ccggccatgc tgttggccgc cgagccgccg ctggtctcgc gggccggccg catcgccgcg
     2461 tagagatcgt cggcctcgtc tgagtcgagc agccgcatcg agcccttctg caagccgcgc
     2521 tcggccagga acgcgtcgtc gcaggtcgcg atcacgtcga cgatcgcatc gccgatggcc
     2581 actatgtcga gctttgggga agtcacggtg gatgggccta gcgtcggctc acgcgggcgg
     2641 tcaactcgcc gtcattgcgg cgagccgaac gactcgttac gccaaccgcc atgaccagcg
     2701 aagtccaggc cgccgcgcgc gcaccggaag cgggcgcagc gccgcaacac ccgctcatca
     2761 gcccgtccct gttcgcgttc gggatcttct acggcggcat ggtgtgcatc gcaggcgtgc
     2821 tcggcaacaa gcaggtcgcc cttgggccgc tggcggtcga ggccggcatt ttcgccttcc
     2881 tgttcctggt catcacctcg agcgcggtcg ccgagctgca cggccggtcg attgccaacc
     2941 ggctggtgct gatcggcttc gtgccgctga tcgcctcgct tctgctgacc ctgctggtgc
     3001 tcggcacccc ggcggccgac gacatggacc cgcggcggct cgaggcgttc gagacggtga
     3061 tgggcgggac cccgcgaatc tggctcggcg ggatcctcgc ctacggcacg tcgcagattt
     3121 tgaacgtcac catcttcagc cggctgaagc gctccggcgg gcggttgctg tggctccgct
     3181 cggcggtcgc cagcgtcttg agccagatcg tcgacacgct cattttcatc accgtcgcct
     3241 tctacggcgt gttcccgatc gccgagctgc tgatgggcca gatgctggcc aaggtcgtgc
     3301 tgtcgctgat cttcgtcccg cccttggtct atctgttcgt ggcgctcggc cgccggctcg
     3361 acgaaagacg agcttagcgt cggttaacgg ttgccaaccg ggcggcgatt tgctcgaaat
     3421 ggttgcgagt cgcgggggtg gcggctttcg atttccgtgt cgaatctgca acggcggcca
     3481 ggaatccggt gacggccgaa atgcttgtca tcgcaatgtc ttgaaccggc tatagctgcc
     3541 cctcgaatct gttctgtgag tcgctcgttc gggggatcat tttccgtgaa aaaatttgct
     3601 ctgctttgct ctacggcatt gttgatgccg gcagccgccc atgcccagtc gaccggctcg
     3661 gtcgacttcg agaaggacgc gatcatcgtc accggcaccc gggtccggga tgtcggcggg
     3721 gtccaggcgc ccgacgccgc caaggccaag gcggtcctga cccaggagat catcgcccgg
     3781 cagaaccccg gccagacgat tctcgacacc atcaacctgg tgccgggggt cagtttccag
     3841 aacaacgacg cctacggctc ctcgggcggc aacctcacca tccgcggttt cgattcgacc
     3901 cgaatcagcc tgacgttcga cggaatccag ctcaacgaca gcggcaatta cgcgatctac
     3961 tcgaaccagc agctcgaccc cgagctgatc gagcaggtca acgtcaatct cggctcgacc
     4021 gacgtcgact cgccgaccgc ctcggcggtg ggagggaccg tcaactaccg caccatgatt
     4081 cccgccagcg atatgaacct gcgtgggctc atttcgctcg gcgaatggga tttccggcgc
     4141 tttttcgccg tctggaacat cggcaccgtc gggccgtggg gcacccgcgc cttcgtgtcg
     4201 gcgtccaagg ccgacaaccg ggtgccgttc aacggctacg gcaagatcga caagcagcag
     4261 tacaacgcgc gcatctggca gccggtcggc gcggccggtg acttcgtgtc ggtctcgggt
     4321 cactacaacc agaatcgcaa caatttcttc ggctcgctgc cgctccggct cgacaccacg
     4381 caaagcacca caaactcggc gccgcgagtg gtcgggtccg gaacgagcaa ccgctatccg
     4441 ggcagccgcg acgagcgctt ctatgacatc aacgacactt gcgtactgga cacgccgcag
     4501 gcgggggtcg ccgatgcgcc cgctccggcg ccgcccaacc aggcgagctg cggcaccgag
     4561 ttcgaccgcc gctacaaccc gtcgaacacc ggcaacattc gcggcgcctc tcgcttcacg
     4621 atcgccgagg gtctcgtcct caccgtcgac ccgagctacc aatatgtgaa ggccaacggc
     4681 ggcggcaccg tgaccggcga agagggcctg cgcgacatca acccggcggg cggcacggcg
     4741 agcgtcgctt cctgccgaac cacgccgatc agtacgaaca accgatgcgt cgcgggctat
     4801 ctcggcggaa cgccgttctt cggccgcgac atcaacggcg acggcgatgt cctcgaccgg
     4861 gtgacggtcc tcgcgccgag ccagacccag acccatcgct acggcgtcat cgccggcctg
     4921 cgctgggagc tcgcgcccag ccacacggtg cgggtcagct atacgctcga ccacgcgcgc
     4981 catcgtcaaa ccggtgaagt cggaatgctg gaaatcgatg gtgagccgga ggacgtcttc
     5041 ccgatcaatg acccggaa
//
LOCUS       DASTZO010000148         2121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_508651,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000148 DASTZO010000000
VERSION     DASTZO010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2121
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_508651"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1165
                     /gene="rimO"
                     /locus_tag="VFU20_04200"
     CDS             <1..1165
                     /gene="rimO"
                     /locus_tag="VFU20_04200"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEU5285699.1"
                     /translation="SAKAESLEAIGEAIAENGRVVVTGCMGKDADVIRARFPGVLAVT
                     GPQQYEEVVAAVHEAAPPVANAFLDLVPEAGLKLTPRHYSYVKIAEGCNHRCAFCIIP
                     SLRGDLVSRRPDAILREAEKLVAAGTRELLVISQDTSAYGVDLRHASWTWRGREVRAH
                     MTDLARELGSLGAWVRLHYVYPYPHVDRVIPLMAEGLVLPYLDIPFQHASPKVLRAMR
                     RPANDARVLQRIAGWRDVCPDIAIRSSFVVGFPGETEEDFQYLLDWLEAAQLDRVGAF
                     RFEPVEGAAANALPDPVPEEVKQERYERIMALSARISAEKLRAKIGKSLEVLIDSVDK
                     ETGGATGRSKADAPEIDGEVHLRDAAHLTPGDIVPALIEDSDEHDLYGVPLNS"
     gene            1258..1734
                     /locus_tag="VFU20_04205"
     CDS             1258..1734
                     /locus_tag="VFU20_04205"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEU5285700.1"
                     /translation="MAFGASAALFGTALAEPVAQGGQKFSMILTDEAEVPGPGDPDAS
                     GFANIIINPGQARVCWEITTSGVNPGYSVAAGSGAHIHRGDATVAGPIVVHLALTLNG
                     TNTGCVDVARPLIEEIRKNPAQFYVNVHWSDQTAPITLTSYAAGALRAQLTKNQLM"
     gene            1811..>2121
                     /locus_tag="VFU20_04210"
     CDS             1811..>2121
                     /locus_tag="VFU20_04210"
                     /inference="COORDINATES: protein motif:HMM:NF045350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEU5285701.1"
                     /translation="MTDFSTMLLADRGRKARAIHIVDKDSFGEWAKGRSAADRALLEA
                     HRFDGKTGYASVILPSGAEFEVVTAVANAASLSPWCLAKLPETLPEGDYRLAKGEPGK
                     AA"
BASE COUNT          361 a          733 c          709 g          318 t
ORIGIN      
        1 ttcggccaag gccgagagcc tcgaggcgat cggcgaggcg atcgccgaga acggccgggt
       61 ggtcgtcacc ggctgcatgg gcaaggacgc cgacgtcatc cgcgcccgct tcccgggcgt
      121 gctcgcggtc accggcccgc agcaatatga ggaggtcgtc gccgcggtcc acgaggcggc
      181 gccgccggtc gccaacgcgt tcctcgacct ggtgcccgaa gccgggctca agctgactcc
      241 ccgccactac agctatgtga agattgccga gggctgcaac caccgctgcg ccttttgcat
      301 catcccctcg ctgcgcggcg acctcgtcag ccgccgcccc gacgcgatcc ttcgcgaggc
      361 ggagaaactg gtcgccgcgg gcacccgcga gctgctggtg atcagccagg acacctcggc
      421 ctacggggtc gacctgcgcc acgcgtcgtg gacctggcgt ggccgcgagg tgcgagcgca
      481 catgaccgac ctcgcgcgcg agctcggctc gctgggggcc tgggtgcggc tgcactatgt
      541 ctacccctac ccgcacgtcg accgggtgat cccgctgatg gccgaggggc tggtgctccc
      601 ctacctcgac atcccgttcc agcacgccag cccgaaagtg ctgcgcgcga tgcgccgccc
      661 ggccaacgac gcgcgggtgc tgcaacggat cgccgggtgg cgggacgtct gccccgacat
      721 cgccatccgc tcgtcgttcg tggtcggctt ccccggtgag accgaggaag atttccaata
      781 tctgctggac tggctcgagg ccgcccagct cgaccgcgtc ggagcgttcc gcttcgagcc
      841 ggtcgagggg gcggccgcca atgcgctgcc cgacccggtg cccgaggagg tcaagcagga
      901 gcgctacgag cggatcatgg cgctgtcggc gcggatttcg gccgaaaagc tgcgagccaa
      961 aatcggtaag tcattggaag tgctgattga ttcggtcgac aaggaaaccg gcggggcgac
     1021 cggccgctcg aaggccgacg cgcccgagat cgacggcgag gtccatttgc gcgacgcggc
     1081 gcatctgacg cccggcgaca tcgtcccggc tttgatcgag gacagcgacg agcacgacct
     1141 ctacggagtg ccgttaaact cataacggaa ccgaatcgct atacgaatct tttagcgcat
     1201 cggggcgggt agatggcgag ggagtaatca gccatgacgc ccaaagctca gcttttattg
     1261 gcgtttggag cctcggcggc gctgttcgga accgcgcttg ccgaaccggt cgcgcaaggc
     1321 ggccaaaagt tctcgatgat cctgaccgac gaagccgagg ttccgggacc gggcgacccg
     1381 gacgccagcg gcttcgccaa catcatcatc aacccgggcc aggcgcgggt ctgctgggaa
     1441 ataaccacct cgggcgtgaa tccgggctat tcggtcgcag ccggctccgg cgcgcacatc
     1501 caccgcggtg acgcgaccgt ggccggtccg attgtcgtcc atctggcgct cacactgaac
     1561 ggcacgaaca ccggctgcgt cgatgtcgcg cgcccgctga tcgaggagat ccgcaagaac
     1621 cccgcgcaat tctatgtgaa cgtccactgg tccgaccaga ccgccccgat cacgctcacc
     1681 agctatgcgg ccggagcgct gcgcgcgcag ctcaccaaga accagctgat gtagctgcac
     1741 ccgacgacgc cctctcccgg cgccgggaga gggttggggt gacggcgttc cttggctaca
     1801 gccccgcccc atgaccgatt tcagcacgat gctcctcgcc gaccgcggcc gcaaggcgcg
     1861 cgccatccac atcgtcgaca aggacagctt cggcgagtgg gcaaagggcc gcagcgccgc
     1921 ggaccgggcc ctgctcgaag cacaccgctt cgacggcaag accggctatg cgagcgtgat
     1981 cctgccgagc ggcgccgagt ttgaggtggt gaccgcagtc gccaacgccg catcgctgtc
     2041 gccctggtgc ctcgccaagc tcccggagac gcttccggaa ggggactatc ggctggccaa
     2101 gggcgagccg ggcaaggcgg c
//
LOCUS       DASTZO010000149         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_349528,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000149 DASTZO010000000
VERSION     DASTZO010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_349528"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFU20_04215"
     CDS             complement(<1..332)
                     /locus_tag="VFU20_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5285702.1"
                     /translation="MSENQPQLHHLDCGGHRLAYRLRRGSAPTLLFLPGYASDMDGAK
                     AVALDAFAGRRGLAMLRFDYSGTGLSAGSFAHGTLERWIDEAQAMAEQLIAGPLIPIG
                     SSMGGWIAL"
     gene            549..1541
                     /locus_tag="VFU20_04220"
     CDS             549..1541
                     /locus_tag="VFU20_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU5285703.1"
                     /translation="MRKYLLAAVAVAAIASPAVARDGAGYIGVEGGVLFPKDNDGDAF
                     VDFTTTQTPATPLAPAGPADTTFEDMFGIDYKTGYDLDLIGGYDFGMFRLEAELGYKR
                     ASVDEFEIDNSDIAALNAALNRPSAAPDPGAPGLPALTADDLDLDGRVSVLSGMVNAL
                     LDFGDDDGMSFYAGGGFGRARVKLFGDSDSAWAYQLIAGARTALSSNIDVGLKYRYFR
                     TGKIGLEDDAIALSGNPNRFVVGTTNVDQTTNGLLLSDFENRFRSHSLLLSLIYNFAP
                     PPPPPPPPPPPPPPPPPEAPATQTCPDGSVILATDMCPPPPPPPPPPPSAGERG"
     gene            1723..2706
                     /locus_tag="VFU20_04225"
     CDS             1723..2706
                     /locus_tag="VFU20_04225"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family protein"
                     /protein_id="HEU5285704.1"
                     /translation="MQYRRLGDSDLEVSEISLGSWLTYGVGVEADAARACLDAAFDQG
                     INFIDTANVYGRGAAETFLGQALEGRPRDSYILATKLFFPMSDEDRGLSRAQVEKQLD
                     ASLERLRTDVIDLYQCHRYDEETELEETMEALTRAVDSGKVRYIGFSEWPAERIQAAL
                     DMAGPSSSLGTRVAKFVSSQPQYSLLWREPEADVIPLCAANGISQIVWSPLGQGVLTG
                     KYDPDRPPPPGTRAADDVMGGWMDRLMDPPVLRAVQRLKPIAGEAGLTLPQFALAWVL
                     REPNVASAIIGASRPQQVVENAAASGAAVDPELFTRAEELIEQALAQEPAG"
BASE COUNT          445 a          971 c          907 g          442 t
ORIGIN      
        1 agcgcgatcc agccgcccat cgacgagccg atcgggatca gcggcccggc gatcagctgc
       61 tcggccatcg cctgcgcctc gtcgatccag cgctcgagcg ttccgtgcgc gaagctgccc
      121 gcggacagac cggtgcccga ataatcgaac cgcagcatcg ccagtcctcg gcgcccggcg
      181 aacgcatcca gcgccaccgc cttggcgccg tccatgtccg acgcatagcc tggcaggaac
      241 agcagggtcg gggccgagcc gcggcgcagc cggtaggcga ggcggtggcc gccgcaatcg
      301 agatggtgga gctgaggctg gttttcgctc attgcgccag cggcttaggc cttagccccg
      361 ccggcggaca ggccaaatcg cccgcgacgc ctcgttgccg ggagcggcga aaagggaccg
      421 caagaatccg gtgtggcctg attgcatcag tttcaatcca attgccggcg ggacgttgct
      481 gggctgaaac tttcagcggg gcgggcggtt agggagccgc caatcaagcg ccataagggg
      541 taaagtacat gcgcaaatat cttctcgccg cagttgccgt cgcggcaatt gcctcaccgg
      601 ccgtcgcccg cgacggggcc ggctatatcg gtgtcgaagg gggcgttctg ttccctaagg
      661 acaatgacgg cgatgccttc gtcgacttca cgaccaccca gacgccggcg acaccgctcg
      721 ctccggccgg gccggccgac acgacgttcg aggacatgtt cggcatcgat tacaagaccg
      781 gttatgacct cgacctgatc ggcggctacg acttcggcat gttccggctc gaagccgagc
      841 tcggctacaa gcgcgccagc gtcgatgaat tcgaaatcga caacagcgat atcgccgcgc
      901 tcaacgcggc cctcaaccgg ccttcggccg ccccggatcc cggagctccc ggccttccgg
      961 cgctgaccgc cgacgatctc gacctcgacg gacgcgtttc ggtcctgtcc ggcatggtca
     1021 acgccttgct cgacttcggc gacgatgacg gcatgtcatt ctacgccggc ggcggtttcg
     1081 gccgcgctcg agtcaagctg ttcggcgatt cggacagcgc ctgggcctac cagctgatcg
     1141 ccggtgcccg caccgcgctt tccagcaaca tcgacgttgg cctgaagtat cgttacttcc
     1201 ggaccggcaa gatcggcctc gaggacgatg cgatcgccct ctcgggcaac ccgaaccggt
     1261 tcgtcgttgg caccaccaac gtcgatcaga cgaccaacgg tctgctgctg tcggacttcg
     1321 agaaccgctt ccgttcgcac agcctgctgc tgagcctgat ctacaacttc gctccgccgc
     1381 cgccgcctcc gccgccgccg ccgccgcctc cgccgccgcc tcctcccgag gctccggcga
     1441 cgcagacctg cccggacggc tcggtgatcc tggcgacgga catgtgcccg ccgccgcctc
     1501 cgcctccgcc tccgccgccg agcgccggcg agcgcggcta aggggaattc ccgcaaagtc
     1561 ggtactttgc gggagcccta aagcgcggac gacccgggca cacgggcgtt acaacttccc
     1621 gggcgaggca cccgcttcgc ccgggatttt ctttgcccgg acgtttcgcg ggaacgctgg
     1681 ggccgcgcgc cggttgagcg tcggacgagg aggcgacgac cgatgcagta ccgcaggctt
     1741 ggtgacagcg atctcgaggt ttcggaaatt tcgctcggct cgtggctgac ctatggagtc
     1801 ggggtcgagg ccgatgccgc ccgcgcctgc ctcgatgccg cgttcgacca aggcatcaac
     1861 ttcatcgaca ccgccaacgt ctacggccgc ggcgccgcgg agaccttcct cggccaagcg
     1921 ctcgagggcc ggccgcgcga cagctatatc ctcgccacca agctgttttt cccgatgagc
     1981 gacgaggacc gcggcctgtc gcgggcccag gtcgaaaagc agctcgacgc gtcgctcgag
     2041 cgccttcgga ccgacgtcat cgatctctac caatgccacc gctacgacga ggaaaccgag
     2101 ctcgaggaga cgatggaggc gctgacccgg gcggtcgaca gcggcaaggt gcgctacatc
     2161 ggcttttccg aatggccggc cgagcgcatc caggccgctc tggacatggc cgggccctcg
     2221 tcttcgctcg ggacgagagt cgccaagttc gtctccagcc agccgcaata ttcgctgctg
     2281 tggcgcgagc cggaggcgga cgtgatcccg ctgtgcgcgg ccaacggcat ttcgcagatc
     2341 gtctggtcgc cgctcggcca gggcgttctg accggcaaat acgatcccga ccggccgccg
     2401 ccgcccggca cccgagcggc ggacgatgtg atgggcggct ggatggaccg gctgatggac
     2461 ccgcccgtgc tgcgcgcggt gcagcggttg aagccgatcg ccggggaagc cgggctgacc
     2521 ctgccgcagt tcgcgctcgc ctgggtgctt cgcgagccca atgtcgcctc ggcgatcatc
     2581 ggcgcgtcgc gtccgcagca ggtggtcgag aatgccgccg cttcgggggc ggccgtcgac
     2641 cccgagctgt tcacccgcgc cgaggagctg atcgagcagg cgctcgcgca ggagccggcc
     2701 ggctagatcg ccttcgagct gcgccgcgcc ggcgagacga agatgtcctc gatcggcagg
     2761 tcgaa
//
LOCUS       DASTZO010000150         7491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_975074,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000150 DASTZO010000000
VERSION     DASTZO010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7491
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_975074"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1843)
                     /locus_tag="VFU20_04230"
     CDS             complement(<1..1843)
                     /locus_tag="VFU20_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEU5285705.1"
                     /translation="MPLTAAASAREILTGLHDVMASRGSAQSKLDKVVDLIAEAMESE
                     VCSIYLLRDNQLELFATHGLRKEAVHVTRLAMGQGLVGTIAQEGRILNLAEAASHPEF
                     VYLPETGEERYHSFAGVPIVRLESPVGVLAVQHAEPRRYEDVEIEALQTVAMVLSEMI
                     AAARLVDGARRGRMRSAGPLRLTGLKLVTGMAKGEAVFHQPRVVVEHTVAEDTEAERA
                     RVYDAFRRMREQIDAMTKEAEFGTAGEHQEILAAYRMFAYDEGWGRRINEAIDSGLTA
                     EAAIERVQQRTRARMREIDDPLLQERMHDLEDLSNRLLRIVSGRMGTAAQTGLTRDTV
                     LIARNLGPAELLEYDRRRLKAVLLEEGSLTAHMTIVARAMGVPVVGRLQDIRHSVNER
                     ETILVDGDNGSVIIRPNRTLTAGFDHRMALSQKRRAQFAAVRSLPAVTKDGLEVSVMV
                     NAGLAEDAAALELTGADGIGLFRTEFQFLVSATLPGRERQQRLYSKVLDAAGDRPVTF
                     RTLDIGGDKALPYLADVKDEAENPAMGWRALRLSLDRSTLMKAQARALIEASAGRVLR
                     VMFPMISEPWEYEEARALVEEQIAWARKSKKKLPSRVEFGAMLEVPSL"
     gene            complement(1878..3185)
                     /locus_tag="VFU20_04235"
     CDS             complement(1878..3185)
                     /locus_tag="VFU20_04235"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5285706.1"
                     /translation="MIRAIVAAIAFLLGAAAGAREPPGLHSALDRLAVEGRFSGAVVI
                     RGPEGIRFARGYGLADPFANRAFTPDTPADSGSLAKPVTAAAVLMLAREGKLDLDAPV
                     RRYLPEYPHASTIVRHLLAHSAGLSIDESERSIVGKTNEALLAEARRGEPLFEPGSGF
                     TYCNLCSVALAILIERVSGSHYLDFARTRLLLPAGVALRPRRLSDWAGRAIGYRRTTD
                     GKVERADSYEDERFYGAANLSVSASQLAEWGTQWWRPPLTSIRSPATTPAMISAKRSG
                     LTWGNWYCAPGGRRCHYLGHHEGFHHMLYWDSERRVSLALVSNNTLAPALQQRLQRAL
                     VAFAEGRSAQARRELAQPLADRPVAPGRYRFPTGEAVVVRSKRKSSLAVDRREIEYPA
                     YRMGAGIRYVPGLDAYLAGAPGGGLRWLSLHEDFRGAPSAPRR"
     gene            3270..4076
                     /locus_tag="VFU20_04240"
     CDS             3270..4076
                     /locus_tag="VFU20_04240"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEU5285707.1"
                     /translation="MPFDVDRPYPIGDDQIDLFRRDGFVRLKQLFAADELQHYGDELV
                     RLTMALNRQTRPLAERSTYGKAFLQITNLWEQGGLAREFVFGRRLAGIAAALLEVDRV
                     RLYHDQSLMKEPGGGITPAHADQYYWPLASDRSITAWVPLQPVPAAMGPIGFYAGSQR
                     FEVGRDLPISDQSERLIAAEMERQGFPLIDDPFELGEVSFHSGWTFHRAGPNLTDRPR
                     AVMTIIYMDRDMRLKAPANRMEQADRDAWCPGAKVGEVIDTPKNPVLFER"
     gene            4251..5366
                     /gene="ugpC"
                     /locus_tag="VFU20_04245"
     CDS             4251..5366
                     /gene="ugpC"
                     /locus_tag="VFU20_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003299457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU5285708.1"
                     /translation="MATVELRGVSKSFGATPVLRDVKLSVGEGEFLVLVGPSGCGKST
                     LLRAIAGLDEVDRGDVLIGDKRVNDLPPAKRGIAMVFQSYALYPHMTLYDNMAFGLKL
                     AGKSKAEIDSAVTDAAELLGIRHLLDRRPKTLSGGQRQRVAIGRAIVRHPSVFLFDEP
                     LSNLDAALRVRMRYEFARLHKRLKTTTVYVTHDQVEAMTLADRIAIIDKGRVEQLGTA
                     EELYERPASLFVAGFIGSPKMNFLEATFETAGPDAATVRLRDGREVEVAVDAAGAGKG
                     DPATLGIRPEHLTLSHTGDGIAASVALIEWLGNMRFAYLATEVADEPLIMQLTSHDEV
                     REGQSVKLLAAPAHCYLFDRGGQAFPRPSQKPGSLAA"
     gene            5544..6539
                     /locus_tag="VFU20_04250"
     CDS             5544..6539
                     /locus_tag="VFU20_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5285709.1"
                     /translation="MARAADVSVATVSRTLNGIDKVAGLTRDRVLRIADELDYVPHSG
                     ARALSTRRTDTIGVLLPDLHGEYFSELIRGIDLAARARGLHLLLSSSHGDPAEAAAAL
                     RAMRSRVDSIIVMLPNAGEELLHSRKGSAPMVFLGSGAHRDAPSFEIDNFAGAFAITR
                     HLLDSGRSRIAFVAGPADNFEARERLRGYRAAIEAAGGAERVVAGDFSEQSGRAAAGE
                     LIGAAPPDAIFCANDMMAIGCLVALRDAGLSVPGDIALAGFDDIPIARYVSPALTTAA
                     VPIAEIGRRALECCIELAAGRPTDRRHLFQPVLAIRSSTQPTDRPTHPGSKGEIS"
     gene            6536..>7491
                     /locus_tag="VFU20_04255"
     CDS             6536..>7491
                     /locus_tag="VFU20_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5285710.1"
                     /translation="MTKSNSRRRLEPNAAFRALLLGVSTITMISAVAVPAAAQTITAS
                     IGGQVRDAQGAAVANAVVTARNESTNQDVTATTDSEGNYRLAGLRPGPYTISTQIGGV
                     AVSDRVTVEIGQSATLDLAPPVAAAEGEAPPAAAATDEIVVTGRRLVETRTSEVATNV
                     SQQQIRRLPQTDRNFLAFTQLAPGVKYNDSETNRGFQSGASTAAAVNVFIDGINLKNS
                     VLDQGVAGQQDSRGNPFAQLAVQEFRVLTQNYKAEYEQAAAAIVTSVSKSGTNQLRGD
                     VFASYTDKSLMEENVFDKRADRPKPDFKRTQYGAALGGPIIA"
BASE COUNT         1188 a         2627 c         2571 g         1105 t
ORIGIN      
        1 cgaggctcgg cacctcgagc atcgcgccga actcgacgcg cgagggaagc ttcttcttcg
       61 acttgcgggc ccaggcgatc tgctcctcga ccagggcgcg cgcctcctca tattcccacg
      121 gctcggagat catcgggaac atcacccgaa gcactcggcc ggcggacgcc tcgatcagcg
      181 cccgggcctg cgccttcatc agcgtcgagc ggtcgagcga caggcgcaga gcgcgccagc
      241 ccatcgccgg gttctcggcc tcgtccttga cgtcggcgag gtagggcagg gccttgtcgc
      301 cgccgatgtc gagcgtccgg aaggtcaccg gccggtcgcc ggcggcgtcg agcaccttcg
      361 aatagagccg ctgctggcgc tcgcggccgg ggagcgtcgc cgagaccagg aactggaact
      421 cggtccggaa caggccgatc ccgtcggcgc cggtcagctc gagcgccgcg gcgtcctcgg
      481 cgagcccggc gttgaccatc acgctgacct cgagcccgtc cttcgtgacc gccggaagcg
      541 agcggaccgc ggcgaactgg gcgcgccgct tctggctcaa ggccatgcga tggtcgaagc
      601 cggcggtcag agtccggttg gggcggatga tgacgctgcc gttgtcgccg tcgacgagga
      661 tggtctcgcg ctcgttgacg ctgtggcgga tgtcctggag gcggccgacg accggcaccc
      721 ccatcgcccg cgcgacgatg gtcatgtgcg cggtcagcga gccttcctcg agcagcacgg
      781 ccttcagccg ccggcggtcg tactcgagca gctcggcggg gccgaggttg cgggcgatca
      841 gcaccgtgtc gcgggtgagg ccggtctgcg ccgcggtgcc catccgcccc gagacgatgc
      901 gcagcagccg gttcgacaaa tcctcgaggt cgtgcatccg ctcctggagc aacgggtcgt
      961 cgatctcgcg catccgggcg cgggtgcgct gctggacccg ctcgatcgcc gcctcggcgg
     1021 tcaggccgct gtcgatcgcc tcgttgatcc gccgccccca gccttcgtcg taggcgaaca
     1081 tgcggtaagc ggcgaggatc tcctggtgct cgccagcggt gccgaactcg gcctccttgg
     1141 tcatcgcgtc gatctgctcg cgcatccgcc ggaacgcgtc gtagacccgc gcgcgctcgg
     1201 cctcggtgtc ctcggcgacg gtgtgctcga cgaccacccg cggctggtgg aacactgctt
     1261 cgcccttggc cattccggtg accagcttga ggcccgtcag tcgcagcggg ccggcgctgc
     1321 gcatccggcc gcggcgggcg ccgtcgacca gccgcgccgc ggcgatcatc tccgacagca
     1381 ccatcgccac cgtctgcaga gcctcgatct cgacgtcctc gtagcggcgc ggctcggcgt
     1441 gctggacggc gagcacgccg accgggcttt cgagccggac gatcgggacg ccggcgaagc
     1501 tgtgatatcg ctcctcgccg gtctcgggca gatagacgaa ctcagggtgg ctggcggcct
     1561 cggcgaggtt gaggatgcgc ccttcctggg cgatcgtccc gaccaggccc tggcccatcg
     1621 ccagccgggt gacgtggacc gcttccttgc gaaggccgtg ggtggcgaac agctcgagct
     1681 ggttgtcgcg cagcaggtag atcgagcaga cctcgctctc catcgcctcg gcgatcaggt
     1741 cgacgacctt gtcgagcttg gactgggcgc tgccccggct cgccatcaca tcatggagcc
     1801 cggtgaggat ttctcgggcg gaggcggcgg cggtcagcgg catgggttgg agcgctatca
     1861 gatggggcgg cggcctgtca cctgcgcggc gctgacggtg cgccccggaa atcctcgtgg
     1921 aggctgagcc agcgcagccc cccgcccggg gcgccggcga ggtaggcatc gagcccgggc
     1981 acgtagcgga tcccggctcc catccgatag gctggatatt cgatctcgcg gcgatcgacg
     2041 gcgaggctcg acttgcgctt gctgcggacg acaaccgcct cgccggtcgg gaaccggtag
     2101 cggcccggcg cgaccgggcg atccgcaagc ggctgcgcaa gctcccgccg ggcctgggca
     2161 ctgcgtcctt cggcgaaggc gaccagcgcg cgctgaagcc gctgctggag cgccggcgcc
     2221 agcgtattgt tgctgaccaa cgccagtgaa acgcggcgct ccgagtccca gtagagcatg
     2281 tggtggaagc cctcgtgatg gccgaggtaa tggcagcggc gcccgcccgg agcgcagtac
     2341 caattccccc acgtcaggcc ggaccgcttt gcggaaatca tggccggcgt tgtcgccggc
     2401 gagcggatcg aagtcagcgg cggccgccac cattgcgtgc cccactcggc gagctgggac
     2461 gcggaaacgc tcaggttcgc ggcgccgtag aaccgctcgt cctcatagct gtcggcgcgc
     2521 tcgaccttgc cgtcggtggt gcggcggtag ccgatcgcgc gcccagccca gtcggaaagc
     2581 cgtctcggcc tcagcgcgac cccggccggg agcaggagcc gcgtgcgggc aaagtcgaga
     2641 taatgcgagc cgctgacgcg ctcaatcaat atcgccagcg cgaccgagca gaggttgcag
     2701 taggtaaagc cgctgccggg ctcgaacagc ggctcgccgc ggcgggcttc ggcaaggagc
     2761 gcctcgttgg tcttgccgac gatcgaacgc tcggattcgt caatgctcag gccggccgaa
     2821 tgggcgagca ggtgccggac aattgtcgaa gcatgcggat attccggaag gtatcgccgg
     2881 acaggggcgt caaggtcgag cttgccctcg cgcgcgagca tgaggaccgc cgcggccgtc
     2941 accggcttgg ccaacgagcc gctatcggcc ggcgtgtcgg gggtgaaggc ccggttcgcg
     3001 aacgggtcgg cgaggccgta gccacgggcg aagcgaatgc cttcgggacc gcgaatgacg
     3061 acggcgccgc tgaaccggcc ttccacggcc agccgatcca gggccgaatg cagtcccggt
     3121 ggttcccgcg ccccggccgc cgctccaagc aggaaggcaa tcgcggcgac aatcgcccgg
     3181 atcatggcgt gcctaccagc ggcggtcagc ggcatgggtt ggagcgctat cagatggcgc
     3241 tgcgccgcgc cacgcactag cattgatcga tgccgttcga tgtcgaccgc ccgtacccca
     3301 tcggcgacga ccagatcgac ttgttccggc gcgacggctt cgttcgcctg aagcagctgt
     3361 tcgccgcgga cgagctgcag cactacggcg atgagctcgt tcgactgacc atggcgctca
     3421 accggcagac gcgcccgctg gcggagcgct cgacctacgg caaggcgttc ctccagatca
     3481 ccaacctgtg ggagcagggc gggctcgccc gggaattcgt gttcggcagg aggctcgcgg
     3541 gaatcgccgc cgcgcttctc gaggtcgaca gagtgcggct ctatcacgac cagtcgctga
     3601 tgaaggagcc gggcggcggg atcaccccgg ctcacgcgga tcaatattac tggccgctcg
     3661 ccagcgaccg atcgatcacc gcctgggtgc cgctccagcc cgtcccggcg gccatgggac
     3721 cgatcggttt ctacgctggg agccagcgct tcgaagtggg gcgcgacctg ccgatttccg
     3781 accaaagcga gcggctgatc gcggccgaga tggagcggca ggggttcccg ctgatcgacg
     3841 atcccttcga gctcggcgaa gtgagcttcc actcgggctg gaccttccac cgcgccgggc
     3901 cgaacctcac cgaccgcccg cgggcggtga tgaccatcat ctacatggac cgggacatgc
     3961 gcctgaaggc gccggccaac cgcatggagc aggcggaccg cgacgcctgg tgccccggcg
     4021 ccaaggtcgg cgaggtgatc gacacgccga agaacccggt gctgttcgag cgctgaccgc
     4081 gtcgttcggc cgccacgaga aactcaacct ggcgcacacg tcctcagagg gtggaaagcg
     4141 gacatgaggt gcagccgcgt ccacctccga gaccgcacgc ggacgcggtt gcgctcttgt
     4201 cctcggccga tcagctgtgc caccacggcg gcggcatcgg gaggtgcttg atggctacgg
     4261 ttgaattgcg cggcgtcagc aagtccttcg gggcgacgcc ggtgcttcgc gacgtcaagc
     4321 tgagcgtcgg cgaaggcgag ttcctggtcc tcgtcggtcc gtccgggtgc ggcaagtcga
     4381 cgcttctgcg cgcgatcgcc ggcctcgacg aggtcgaccg cggcgacgtg ctgatcggcg
     4441 acaagagggt caacgacctg ccgccggcca agcgcggcat cgcgatggtg ttccagtcct
     4501 acgctttgta cccgcacatg acgctatacg ataatatggc gttcgggctg aagctcgccg
     4561 gcaagagcaa ggcggagatc gactcggcgg tcaccgacgc agccgagctg ctcggaatcc
     4621 gccacctgct cgaccgccgc ccgaaaactc tgtccggcgg gcagcggcag cgggtcgcga
     4681 tcggccgcgc gatcgtccgc cacccgtcgg tgttcctgtt cgacgagccc ttatcgaacc
     4741 tcgacgcggc gcttcgcgtg cggatgcgct acgagttcgc gcggctccac aagcggctca
     4801 agacgaccac cgtctacgtg acgcacgacc aggtcgaggc gatgacgctc gccgaccgga
     4861 tcgcgatcat cgacaagggc cgggtcgagc agctcggcac cgccgaggag ctgtacgagc
     4921 ggccggcgtc gctgttcgtc gcgggcttca tcggctcgcc gaaaatgaac ttcctcgagg
     4981 cgacgttcga aaccgcgggt ccggacgccg cgacggtcag gctgcgcgac gggcgggagg
     5041 tcgaagtggc ggtcgatgcg gccggcgccg gcaagggcga tccggcgacc ctcgggatcc
     5101 ggcccgagca tctgaccctg tcgcacaccg gcgacgggat cgcggcgagc gtcgcgctga
     5161 tcgaatggct cggcaacatg cgcttcgcct acctcgccac cgaggtcgcg gacgagccgc
     5221 tgatcatgca gctgacgtcg cacgacgagg tccgcgaagg ccagtcggtg aagctgcttg
     5281 cggcgcctgc gcactgctat ctgttcgacc gcggcggcca ggcgttcccg aggccttcgc
     5341 aaaagcccgg atccttggcg gcctgattca aaaacccgcc ggacaataga cacttgcagt
     5401 ccggcggaga cctgatacgg tcgctcatgt aaccgtttac accaaagcgg catccatagc
     5461 tgttcggggc gccccgtgga ccattccaga accagcccaa gcatccggcg ggaacccgat
     5521 tgaaaggcgc caccatccgc gacgtggctc gggccgcgga tgtttcggtg gcaacggtgt
     5581 cgcgcaccct caacggaatc gacaaggtcg ccggcctgac ccgcgaccgg gtgctgcgga
     5641 tcgccgatga actcgactat gtcccgcata gcggcgcccg ggccctgtcg acccggcgca
     5701 ccgacaccat cggggtgctg ctccccgacc ttcacggcga atatttttcc gagctgatcc
     5761 gcggcatcga cctcgccgcc cgcgcgcgcg ggctgcacct gctgctgtcc agttcacacg
     5821 gcgacccggc cgaggccgcc gcggcgcttc gggcgatgcg cagccgggtc gattcgatca
     5881 tcgtcatgct gcccaacgcc ggcgaggagc tgctccattc gcgcaagggc tcggcgccga
     5941 tggtgttcct cggcagcggc gcccaccgcg acgccccgtc gttcgaaatc gacaatttcg
     6001 ccggcgcgtt cgcgatcacc cggcacctgc tcgacagcgg ccgcagccgg atcgccttcg
     6061 tcgccgggcc ggccgacaat ttcgaggccc gcgagcggct tcgcggctac cgcgccgcga
     6121 tcgaggcggc gggcggcgcc gagcgggtcg tcgccggcga cttcagcgag cagtcgggcc
     6181 gcgcggcggc gggcgagctc atcggcgcgg cgcctccgga cgcgatcttc tgcgccaacg
     6241 acatgatggc gatcggctgc ctggtggcgc tccgcgacgc gggcctgagc gtccccgggg
     6301 acatcgcgct cgccggcttc gacgacatcc cgatcgcccg ctacgtgagt cccgcgctga
     6361 ccaccgccgc ggtgccgatc gccgagatcg gccgacgcgc gctcgaatgc tgcatcgagc
     6421 tggccgccgg ccgcccgacc gaccgccggc acctgttcca gccggtgctt gccatccgtt
     6481 cgtcaaccca gccaaccgac cgaccgacac atccaggatc caaaggggag atctcatgac
     6541 caagtccaac agccgccgcc gcctcgagcc caacgccgcc tttcgcgcgc ttctgctcgg
     6601 cgtatcgacc atcacgatga tcagcgccgt cgcggtcccc gcggccgctc agacgatcac
     6661 cgcgtcgatc ggcggccagg tgcgcgacgc ccagggggcg gccgttgcca acgccgtggt
     6721 cacggcccgt aacgagagca ccaaccagga cgtcaccgcg accaccgaca gcgaaggcaa
     6781 ttaccggctc gccgggttgc ggcccgggcc ctacacgatc tcgacccaga tcggcggcgt
     6841 tgccgtcagc gaccgggtga cggttgagat cggccagagc gcgacgctcg atttggcgcc
     6901 gccggttgcc gcggccgagg gcgaggcgcc gccggcggcc gccgccaccg acgagatcgt
     6961 cgtcaccggc cggcggctgg tcgaaacccg caccagcgaa gtcgcgacca acgtcagcca
     7021 gcagcaaatc cgccggctgc cgcagaccga ccgcaacttc ctcgccttca cccagctcgc
     7081 tccgggcgtg aaatataacg acagcgagac caaccgcggc ttccagagcg gcgcgtcgac
     7141 cgcggccgcg gtcaacgtat tcatcgacgg catcaacctc aagaacagcg tgctcgacca
     7201 aggcgtggcc ggccagcagg acagccgcgg gaacccgttc gcccagctgg cggtgcagga
     7261 attccgggtc ctgacccaga actacaaggc cgaatatgag caggccgcgg cggccatcgt
     7321 cacctcggtg tccaagtcgg ggaccaacca gctgcgcggc gacgtgttcg cctcctacac
     7381 cgacaagagc ctgatggagg agaatgtctt cgacaagcgg gccgaccgtc ccaagcccga
     7441 cttcaagcgc acccagtacg gggcggcgct gggtgggccg atcatcgccg a
//
LOCUS       DASTZO010000151         4848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_794280,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000151 DASTZO010000000
VERSION     DASTZO010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4848
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_794280"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..775
                     /locus_tag="VFU20_04260"
     CDS             <1..775
                     /locus_tag="VFU20_04260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU5285711.1"
                     /translation="AFRLAVPEPEPAHNEPQDIPLTIVFEDEHLLVVDKPAGLVVHPA
                     AGNRDRTLVNALLHHCGASLSGIGGVARPGIVHRIDKDTSGLLVVAKTDVAHEGLARQ
                     FARHSIERRYLAIVAGVPKTAGGMVDAPLARSSANRKKIAVVKARDDGGRGKRAITHW
                     KRLALLRDAALVECRLETGRTHQVRVHMASIGHPLLGDSIYGGVGKTHRKLLNELGFH
                     RQALHAAGLGFTHPVTRHRLSFESGMPADMQELFTALGV"
     gene            831..1739
                     /gene="rpoH"
                     /locus_tag="VFU20_04265"
     CDS             831..1739
                     /gene="rpoH"
                     /locus_tag="VFU20_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HEU5285712.1"
                     /translation="MAQQKGIVSIPASGGEAGLNRYLSEIKKFPILAPEEEYMLAKRW
                     REHGDTEAAAKLVNSHLRLVAKIAMGYRGYGLPVSELISEGNIGLMQGVKKFEPDRGF
                     RLATYAMWWIRASIQEFILRSWSLVKMGTTAAQKKLFFNLRRMKNQIDAFEEGDLKPA
                     DVTKIATDLGVTEDEVVSMNRRMAMGGDTSLNVSLKGDEGGESQWQDFLVDTGPLQDE
                     RIAEDEESRVRHDLLMDALESLNDREKHILMERRLTDEPKTLEELSQVYDVSRERIRQ
                     IEVRAFEKLQAALLRLAGDRKLLPAA"
     gene            1907..3949
                     /locus_tag="VFU20_04270"
     CDS             1907..3949
                     /locus_tag="VFU20_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5285713.1"
                     /translation="MGAETRSGETKGTRAIALVGPAGAGKTSLAEAMLFAAGATDRLG
                     STANGSSIGDSSPEARARGGSTELNLYNFDYLGDRFALIDCPGSVGFAADGARALALA
                     DMAILVVDPDPARAPLAAPALRALDELGIPHLIFVNRIDQAHGRIRDLLSALQPMSVS
                     PLIARQIPIRDGEKVTGFVDLALERAFHYRPGKDSEQIEMPPEVQQREAEARTHMLEQ
                     LADHDDELLEQLLMDETPSRDRILQDLARETGESLGVPVLFGSASSAWGVGRLLKALR
                     HESPGPDATARRLSVDSPALHVFKIHHGGSVGRLALARALGGPIAEGSDLKTANGSIA
                     RLGALFRMQGDKTQKVAEARDGDVVAVAKIDAVKAGEWLGAGKLPPPIESELPARNYA
                     LAIEPADRKDDVRMSGALQRLLEEDSALVLEQDEASHEMRLKGVNDEHLNTVLARLKR
                     RYGVEVKSHQPTIGYRESIRRTVTQKGRHKKQSGGHGQFGDVVIEVRPLSRGEGFQFH
                     DKITGGAIPRQWIPAVEHGVREAMQKGPLGFPVVDVAVTLVDGSYHSVDSSELAFRMA
                     GRIAMNEALAAAHPHLLEPVQRLTVVCPSSATSRITSAIASRRGQMLGMAPRDGWTGW
                     DRVDALIPEAELGGLEAELRSQSHGLATYEAEFDHLAELNGTLADKIVQQRMPEPA"
     gene            4023..>4848
                     /locus_tag="VFU20_04275"
     CDS             4023..>4848
                     /locus_tag="VFU20_04275"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU5285714.1"
                     /translation="MRHWFKDRHFKSLLKNSSYLAVSKAVAAVAALATLAFAGRSLGV
                     LMFGMLILITSYAKAASGLSKFQSWQLIVRYGGQALEAGETTEFKASTGFGLALDVVS
                     GFGGMILAILLLPLIGGWVGIEDRYLVAAMLYCTLLPTMSAAVPVGVLRSLDRFDLLS
                     WQGTATPITRAILAGFGWWLDAPFEAFVAIWYVTELGGDLYLWFLAWRELKRRDLIKG
                     IRPTLRPQQLPGAWRFAIHVNLTSSLTAAWGPIARLIIGGLVGPAGAAIYRVAASLA"
BASE COUNT          818 a         1662 c         1667 g          701 t
ORIGIN      
        1 ggcgttccgg ctggcggtcc ccgagcccga gcccgcgcac aacgagccgc aggatatccc
       61 gctgacgatc gtgttcgagg acgagcatct gctggtcgtc gacaagcccg ccggcctcgt
      121 cgtccatccc gccgccggca accgcgaccg gaccctggtc aacgctttgc tccaccattg
      181 cggcgccagc ctgtcgggga tcggcggggt cgcgcggccg gggattgtcc accgaatcga
      241 caaggacacg tcggggctgc tggtggtcgc caagaccgac gtcgcccacg aagggctggc
      301 caggcagttc gccaggcaca gcatcgagcg gcgttatctg gcgatcgtcg ccggcgttcc
      361 gaagacggcc ggcggaatgg tcgacgcgcc gctcgcccgc tcgtcggcca accgcaagaa
      421 gatcgcggtg gtgaaagcca gggacgatgg cggccgcggc aagcgcgcca tcacccactg
      481 gaaacggctt gcattgctgc gcgacgcggc gctggtcgag tgccggctcg agaccggccg
      541 gactcaccag gtgcgggtgc acatggcctc gatcggccac ccgctgctcg gcgactcgat
      601 ttacggcggt gttggcaaga cgcaccgcaa gcttttgaac gagcttgggt ttcaccggca
      661 ggctttgcac gccgccgggc tcgggttcac ccatccggtg acccggcacc gcttgtcgtt
      721 cgagagcggc atgccagccg acatgcagga actgttcact gctcttggtg tataggattg
      781 gaagcgctag ggcgccgcac aagcgctcgg cccagggaga agacgacggc atggcacagc
      841 agaagggcat cgtctcgatc cccgcgtccg gcggggaggc cgggctcaac cgctacctga
      901 gcgaaatcaa gaaattccca atcctcgccc ccgaggagga gtatatgctc gccaagcgct
      961 ggcgcgagca tggcgacacc gaggcggcgg cgaagctggt caactcgcac ctccggctcg
     1021 tggccaagat cgccatgggc taccgcggct acgggctgcc ggtcagcgaa ctgatctccg
     1081 agggcaacat cggcctgatg cagggcgtca agaagttcga gcccgaccgc ggcttccggc
     1141 tcgcgaccta cgcgatgtgg tggatccgcg cctcgatcca ggaattcatc ctgcgctcgt
     1201 ggagcctggt gaagatgggc accaccgccg cccagaagaa attgttcttc aacctgaggc
     1261 ggatgaagaa ccagatcgat gcgttcgagg agggcgacct caagcccgcc gacgtcacca
     1321 agatcgccac cgacctcggg gtgaccgagg acgaggtggt gtcgatgaac cggcgcatgg
     1381 cgatgggcgg cgatacgtcg ctcaacgttt cgctcaaggg cgatgaaggc ggcgagagcc
     1441 agtggcagga cttcctggtc gacaccgggc ccttgcagga cgagcgcatc gccgaggacg
     1501 aggaaagccg ggtgcggcac gacctgctga tggacgcgct ggaatccctg aacgaccgcg
     1561 agaagcacat cctgatggag cggcggctga ccgacgagcc caagacgctc gaggaactga
     1621 gccaggtcta tgacgtcagc cgcgagcgaa tccgccagat cgaggtgcgg gcgttcgaaa
     1681 aactgcaggc ggcgctgctg cggctggccg gcgacagaaa gctcctgccc gccgcctgac
     1741 cggcaaggcg atggttcgtc gcatctgctc ctcgcaatga cgctgccgta acaagcccct
     1801 cttgccaacg gccgaaaacc gtccgacggt gatcgtttcg aagagcgcgc aaggtgcgcc
     1861 ttcaggcgac tcgcgcgccc gacaaccggg acgagaggag agtcgcatgg gcgctgaaac
     1921 cagaagcgga gaaaccaaag gcacccgagc gatcgcgctg gtcggtccgg ccggcgcggg
     1981 aaagaccagc ctcgccgaag cgatgctgtt cgcagccgga gcgaccgacc gcctggggtc
     2041 gaccgccaac ggctccagca tcggcgacag cagcccggaa gcgcgcgccc gcggcggctc
     2101 gaccgagctc aacctctaca atttcgacta tctcggcgac cgcttcgctt tgatcgactg
     2161 ccccggctcg gtcggcttcg ccgccgacgg cgcccgcgcg ctggcgctgg ccgacatggc
     2221 gatcctggtc gtcgacccgg acccggcgcg ggcgccgctc gccgcccctg ccctcagggc
     2281 gctcgacgag ctcggcatcc cgcacctgat tttcgtcaat cggatcgacc aggcccacgg
     2341 ccggatccgc gacctgctgt cggcgcttca gccgatgagc gtgtcgccgc tgatcgcccg
     2401 ccagatcccg atccgcgacg gcgagaaggt caccggcttc gtcgacctcg cgctcgagcg
     2461 ggcgttccac taccggcccg gcaaggacag cgagcagatc gagatgccgc ccgaggtcca
     2521 gcagcgcgag gccgaggccc gcacgcacat gctcgagcaa ctggccgacc atgacgacga
     2581 actgctcgag caattgctga tggacgagac cccgagccgc gaccggattc tccaggacct
     2641 ggcgcgcgag acgggcgaaa gcctcggggt gccggtgctg ttcggctcgg cgagcagcgc
     2701 ctggggggtc ggccgcctct tgaaggcgct gcgccacgag tcgccgggcc cggacgcgac
     2761 cgcccgccgc ctgtcggtgg actcaccggc gttgcatgtg ttcaagatcc accacggcgg
     2821 ctcggtcggc cggctggccc tggcgagggc gctcggcggg cccatcgccg aaggctccga
     2881 cctcaagacc gcgaacggct cgatcgcgcg gctcggcgct ttgttccgga tgcagggcga
     2941 caagacgcag aaggtcgccg aggcgcgcga cggcgacgtc gtcgcggtcg ccaagatcga
     3001 cgcggtcaag gccggcgagt ggctcggggc cggcaagctt ccgccgccga tcgaaagcga
     3061 gctcccggcg cgcaactatg cgctcgcgat cgagcccgcc gaccgcaagg acgacgtccg
     3121 gatgtccggg gcactccagc gcttgctcga ggaggacagc gcgctggtcc tcgagcagga
     3181 cgaggcgagc cacgaaatgc ggctcaaggg cgtcaacgac gagcatctga acaccgtcct
     3241 ggcgcggctg aagcggcgct acggggtcga ggtgaaatcg caccagccga ccatcggcta
     3301 ccgcgaatcg atccgccgca cggtcaccca gaaaggccgc cacaagaagc agtcgggcgg
     3361 ccacggccag ttcggcgacg tggtgatcga agtccggccg ctgtcacgcg gcgagggttt
     3421 ccagttccac gacaagatca ctggcggagc gatcccgcgc cagtggatcc cggcggtcga
     3481 gcatggcgtc cgggaagcga tgcagaaagg cccgctcggc ttcccggtgg tcgatgtcgc
     3541 cgtgaccctg gtcgacggca gctaccacag cgtcgacagc tccgagctcg ccttccggat
     3601 ggccggtcgg atcgcgatga acgaggcgct cgccgccgcc cacccgcacc tgctcgagcc
     3661 ggtgcagcgg ctgaccgtcg tctgcccgtc gagcgcgacc agccggatca cctcggcgat
     3721 cgccagccgc cgcggccaga tgctcggcat ggcgccgcgc gacggctgga ccggctggga
     3781 ccgggtcgac gcgctgatcc ccgaggccga gctcggcggg ctcgaagccg agctccgctc
     3841 acagagccac ggcctggcga cctacgaagc ggagttcgat cacctcgccg agctgaacgg
     3901 aaccctggcc gacaagatcg tccagcagcg gatgcccgag ccggcgtaac cgtttcgcgg
     3961 ggcgacaggg cgggaccttt cgcctagatg cggcgcgcac ccggggagga cgcggcgcgc
     4021 cgatgaggca ttggttcaag gatcggcatt tcaagtcgct gctcaagaac agcagctatc
     4081 ttgctgtgtc caaagcggtt gcggccgttg cagcgctcgc caccctggct ttcgccggcc
     4141 gctcgctggg cgtgctgatg ttcggcatgc tgatcctcat caccagctac gccaaggcgg
     4201 cgagcgggct cagcaagttc cagtcctggc agctgatcgt gcgctacggc ggccaggcgc
     4261 tggaggccgg cgaaaccacc gagttcaagg cctcgaccgg tttcggcctg gcgctcgacg
     4321 tcgtcagcgg cttcggcggc atgatcctcg ccatcctgct gctgccgctg atcggcggct
     4381 gggtcgggat cgaggaccgg tacctggtcg cggccatgct ctactgcacg cttcttccga
     4441 cgatgagcgc cgcggtgccg gtcggggtgc tgcgctcgct cgaccggttc gacctattga
     4501 gctggcaggg caccgccacg ccgatcaccc gggcgatcct cgccggcttc ggctggtggc
     4561 tggacgcacc gttcgaggcc ttcgtcgcca tctggtacgt gaccgagctc ggcggcgacc
     4621 tctatctgtg gttcctcgcc tggcgcgagc tcaagcgccg cgacctgatc aagggcatcc
     4681 gcccgacgct tcggccgcaa cagttgcccg gagcgtggcg cttcgccatc cacgtcaacc
     4741 tgacctccag cctgaccgcg gcctgggggc cgatcgcccg gctgatcatc ggcggcctgg
     4801 tcggcccggc gggggcggcg atctaccggg tcgcggcgag cctcgccg
//
LOCUS       DASTZO010000152         3443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_482840,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000152 DASTZO010000000
VERSION     DASTZO010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482840"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="VFU20_04280"
     CDS             complement(<1..445)
                     /locus_tag="VFU20_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HEU5285715.1"
                     /translation="MPISFDPGRPAGEARIVVAMSGGVDSSVVAALAAQSGAEVIGVT
                     LQLYDHGEAAKRSGSCCAGQDIYDAKAVADRLGIAHYVLDYESRFRTGVIDRFADAYA
                     QGRTPVPCSLCNQGVKFTDLIAFARELGADCLATGHYVRRIVRNGR"
     gene            517..825
                     /locus_tag="VFU20_04285"
     CDS             517..825
                     /locus_tag="VFU20_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285716.1"
                     /translation="MSRFEGDFAAAGGAGLNNGELLDLLAAFSELQGRERQEAIACYL
                     SASEGWHEAMKALRGTFAADAAEAVRLNAAKQDNRDINHRTSEYLQWQALKPAGTDNE
                     "
     gene            841..1140
                     /locus_tag="VFU20_04290"
     CDS             841..1140
                     /locus_tag="VFU20_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008995171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1153 domain-containing protein"
                     /protein_id="HEU5285717.1"
                     /translation="MLENQKFRPAQVIGPLGEPLTLDSLPPPQTSRWVVRRKAEVVAA
                     VAGGLLSVDEACQRYSLSLEEFTSWQRAVDRSGMPGLRVTRIKHYRDVYERQQRY"
     gene            1320..1580
                     /locus_tag="VFU20_04295"
     CDS             1320..1580
                     /locus_tag="VFU20_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5285718.1"
                     /translation="MGFIVWLIVGGIIGWLASLVMRTDGQQGIILNIVVGIVGAVIAG
                     LIFGGGNINTSDPLDLTNILYSLLGAIILLAIVNLVRRGSVR"
     gene            complement(1644..2366)
                     /locus_tag="VFU20_04300"
     CDS             complement(1644..2366)
                     /locus_tag="VFU20_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HEU5285719.1"
                     /translation="MKHAFLAAAALAALAAPVAAQGPAATPFVQSISGTRLDVSATGE
                     VSRVPDLAIVSAGVVTRSASATGAISENAARMERVRAALKRAGIADRDIQTSNISLNP
                     EYRYQENMPPQLTGYTASNQVSVRFRDIRNTGRILDALVAEGANQINGPSLTIDKPEA
                     ALDEARSKAVAAGRARAELYARALGMRVVRLLSVSEGGGYSVPPPMPMMRAEARGMAA
                     DTKIDPGEQQLQVTLAMSFELQ"
     gene            complement(2455..2721)
                     /locus_tag="VFU20_04305"
     CDS             complement(2455..2721)
                     /locus_tag="VFU20_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEU5285720.1"
                     /translation="MMESYGIIGWIVVGLLAGGLAKLLMPGRDPGGCIVTILLGIAGA
                     LLAGFLGQAVGWYGPGEGAGFIAAVIGAFIILFIYRLVLRRRTP"
     gene            2962..>3443
                     /locus_tag="VFU20_04310"
     CDS             2962..>3443
                     /locus_tag="VFU20_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HEU5285721.1"
                     /translation="MNRETTFRSSDTLIVTDGSRRKRGAIIGAVVAVLALVLAYLLFS
                     GGDEQPGAGAGADGRRGQIPTVTVIVPGRSEVARTVTASGALAARRDQPVGVAGEGGM
                     VTRVSVDAGAWVRQGQVLATVDRSVQSQEAAQLAAQVQVARADAALAQNELDRAQALV
                     A"
BASE COUNT          556 a         1137 c         1194 g          556 t
ORIGIN      
        1 atcggccatt ccggacgatt ctccgcacat aatggccggt cgccaggcag tcggcgccga
       61 gctcgcgggc gaaggcgatc aggtcggtga acttcacgcc ctggttgcac aggctgcacg
      121 gcaccggcgt ccggccctgc gcataagcgt cggcgaaacg gtcgatcacc ccggtccgga
      181 agcggctttc gtagtcgagc acgtaatggg cgattccgag ccggtcggcg accgccttgg
      241 cgtcgtagat gtcctggccc gcgcagcacg agcccgagcg cttcgccgcc tcgccgtggt
      301 cgtagagctg gagcgtcacc ccgatcacct cggcgccgct ttgcgcggcg agcgcggcca
      361 cgaccgagct gtcgacgccg cccgacatgg cgacgacgat tcgcgcttcg cccgcggggc
      421 ggccaggatc gaagctgatc ggcatcgccg ctctttaccc gaagttcacc catcgcggct
      481 agacgcgagc aaggaatggc cgggagaggg ctgcctttgt cacgtttcga gggcgatttt
      541 gccgccgccg ggggcgccgg gctgaacaac ggcgagctgc tggacctgct ggcggccttt
      601 tcggagctgc agggccgcga gcgccaggag gcgatcgcct gctatctgtc ggcgagcgag
      661 ggctggcacg aggcgatgaa ggcgctccgc gggacgttcg cggccgatgc cgcggaggcg
      721 gtaaggttaa acgccgctaa gcaggataat cgggatatta accaccgcac ttcagaatat
      781 cttcaatggc aagctcttaa gcctgctggg accgacaatg aataagcagg tttgacctca
      841 atgctcgaga accagaaatt ccggccagcc caggtgatcg gcccgctcgg cgagccgctg
      901 acgctcgaca gcctgccgcc tccccagacc agccgctggg tggttcgccg caaggccgag
      961 gtcgtggccg cggtcgcggg cggcctgctg agcgtcgacg aggcgtgcca gcgctattcg
     1021 ctgagcctcg aggaattcac cagctggcag cgtgcggtcg accgcagcgg catgcccggc
     1081 ctgcgcgtca cccggatcaa gcattaccgc gacgtttacg agcgccagca gcgctactga
     1141 cggttttgcc gttacggata ttaaccgagc agcagttttc cactgttttc agcgttccag
     1201 ccgagccatc gcactggaca cggccctcca aatccggaac aagacgcgag catcacgcgt
     1261 cttgatgccg gaaggccgtc gcgtacccat tagacggcca cgaacaggag ggaacgctca
     1321 tgggtttcat cgtttggctg atcgtcgggg gtattatcgg ctggcttgcc agcctggtca
     1381 tgcgcacgga cggtcaacaa ggcatcatct tgaacatcgt cgtcggaatc gtcggcgcag
     1441 tcattgccgg cctgatcttc ggcggcggca acatcaacac ttcggacccg ctcgatctca
     1501 ccaacatcct ctattcgctg cttggtgcga tcatcttgct ggcgatcgtc aacctggttc
     1561 gccggggctc ggtccgctag tcgcgacgca acattgaatt cggaaggccg cgcggcactg
     1621 cccgcgcggc ctttttcttt gccctattga agctcgaagc tcatcgccag ggtcacctgg
     1681 agctgctgct cgccggggtc gatcttggtg tcggcggcca ttccccgggc ttcggcgcgc
     1741 atcatcggca tcggcggcgg gaccgaatag ccgccgccct cgctgaccga cagcaggcgg
     1801 accacccgca tgccgagcgc ccgggcataa agctcggccc gggcgcggcc ggcggcgacc
     1861 gccttggaac gcgcctcgtc gagcgccgct tccggcttgt cgatggtcag cgacgggccg
     1921 ttgatctggt tggcgccctc ggcgaccagc gcatcgagga tccgcccggt gttgcggatg
     1981 tcgcggaagc ggacgctgac ctggttcgac gcggtgtagc cggtcagctg cggcggcatg
     2041 ttctcctggt agcggtattc ggggttgagg ctgatgttcg acgtctggat gtcgcggtcg
     2101 gcgatcccgg cgcgcttcag cgccgcgcgg acccgttcca tccgggcggc attctccgag
     2161 atcgctccgg tggcgctggc cgagcgggtc acgaccccgg ccgagacgat cgcgaggtcg
     2221 ggcacccggc tgacctcgcc ggtcgcgctg acgtcgagcc gggtcccgga gatggactgg
     2281 acgaacggcg tcgccgcggg gccctgagca gcgaccggag cggcgagagc ggcgagcgcc
     2341 gcggcggcga gaaaagcgtg cttcatccgg aattcctaat tgcgcatggt cgcgccgcgc
     2401 gagtttgccc gcgtgccatg gctggccgat gaacggctcg gatggcgggc cggatcaggg
     2461 cgtccggcgg cgcagcacca ggcggtagat gaacaggatg atgaaggcgc cgatgacggc
     2521 ggcgatgaag cccgcgccct cgccgggccc gtaccatccg actgcctgac cgaggaagcc
     2581 ggcgagcagc gcgccggcga tgccgagcaa gatggtgacg atgcagccgc ccggatcgcg
     2641 cccgggcatc agcagcttgg cgagcccgcc ggccagcagg ccgacgacga tccagccaat
     2701 aatgccgtaa ctttccatca tgtcgcgctt cccctttttc cgatcgccgc gccacagcgg
     2761 gcaccgccga accaatcatg cgccaacgcg ccttggcgga gcaagtgccg ccgttggtcg
     2821 aaaaaccgtg gcaatgcgcg caggcggtga cagtggcgat gacagtgggc gtctatccgt
     2881 gttgcggtcg gtgagttatt gcgataatac agctgcggca cgaagcgcag cggattcgca
     2941 gggcagggca aagatttgga catgaaccgg gaaaccacgt ttcgctcttc ggacacgctg
     3001 atcgtgaccg acggctcgcg ccgcaagcgc ggtgcgatca tcggcgcggt cgtcgccgtc
     3061 ctcgcgctgg tccttgccta tctgctgttc agcggcggcg acgagcagcc gggagccggc
     3121 gccggagccg acggccgccg cggccagatc ccgacggtca ccgtcattgt cccgggccgc
     3181 agcgaggtcg cgcggacggt caccgccagc ggggcgctgg ccgcccgccg cgaccagccg
     3241 gtcggagtcg ccggcgaggg cgggatggtc acccgggtat cggtcgatgc cggagcgtgg
     3301 gtccggcagg gccaggtgct cgccaccgtc gaccgctcgg tgcagagcca ggaggcggcc
     3361 cagctcgcgg cccaggtcca ggtcgcccgg gccgacgcgg cgctggccca gaacgagctc
     3421 gaccgggccc aggcgctggt cgc
//
LOCUS       DASTZO010000153         7342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_900558,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000153 DASTZO010000000
VERSION     DASTZO010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7342
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900558"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..589
                     /gene="tmk"
                     /locus_tag="VFU20_04315"
     CDS             <1..589
                     /gene="tmk"
                     /locus_tag="VFU20_04315"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU5285722.1"
                     /translation="EGVGKSTQVRALAAALRERGIGVVETREPGGSEGAERIRELLLT
                     GDDDRWGPQAEALLFAAARTDHVDKVIRPAIQAGKWVLSDRFVDSSLAYQGGARELGI
                     EAVRAVNAFGIADCFPDRTLVLALAEGGARARARDAEDSDRIGGRPENYHQKVDLAFR
                     LIAAEDPERVRIVDASGEPGEVTQRLLDAIADLLP"
     gene            586..1560
                     /locus_tag="VFU20_04320"
     CDS             586..1560
                     /locus_tag="VFU20_04320"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEU5285723.1"
                     /translation="MIAGHDRQVAQFEQAWRAGTLHHAWLLAGPKGVGKAHFARAAAT
                     RVLADAAGHAVALPGLETPEEHPIARLVEAGSHPDMRRLERLENPKTGALARNITVDQ
                     VRGLAELFDLTPSMSPWRAVVIDAADDLEKSAANALLKMLEEPPANCLFLLVSHAPGR
                     LLPTIRSRCRRLDFQPLADDAMTSVLESQLPSLSEAERSRIVARAGGSVGRALAFAAL
                     DLAKLEDAALKILREGDPTNARRSELAQELGRKGAGERYAAFLDLVPALIARQARGLE
                     GPGLGRALDAYERSRELAALAPRVSLDPASTVFQLGGILASVVARPVP"
     gene            1596..3101
                     /gene="metG"
                     /locus_tag="VFU20_04325"
     CDS             1596..3101
                     /gene="metG"
                     /locus_tag="VFU20_04325"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEU5285724.1"
                     /translation="MADPFYITTAISYPNGPPHIGHAYEAIAGDVIARFQRAQGRDVR
                     FQTGTDEHGLKMAQAARSEGVEPRDYAKKMSSIFQEMCDVLNVSYDRFIRTSEPEHHR
                     ASQAIWSAMEAKGDLYLGRYEGWYSVRDEAFYDESELTLAQGAGEGAERLSPQGSPVE
                     WTAEESWFFRLSAYQQRLLDHYEANPGFIRPESRRNEVLRFVEGGLSDLSISRTSFDW
                     GVPAPGAPGHVMYVWLDALTNYITGLGYPDETELFARYWPADVHLIGKDVVRFHAVYW
                     PAFLMSAGIELPKMVYGHGFLLSSGEKMSKSVGNVVDPFALADRFGVDSLRYFLLREV
                     PFGQDGSYSPEAIVNRVNAELANSFGNLAQRTLSMVYKNLAGEVPQPGAAAEDVALLD
                     AVRSACERDVPAEFERFAFASGLEAWMGAVFACNAYVDAAAPWALRKSDPGRMAEVLG
                     TLVEAIVILAAAVEPVIPGSARRLIDFIRKGGPIGQPEPIFPRLELEEAAP"
     gene            3112..3891
                     /locus_tag="VFU20_04330"
     CDS             3112..3891
                     /locus_tag="VFU20_04330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU5285725.1"
                     /translation="MRLIDSHCHLNYPGLVERQDEVLDSARERGVGGFLNIATRRSEW
                     GDIIATAERHADVWASVGVHPHEADAHPDLGAAALVEGSEHDKVVAIGECGLDYYYDN
                     SARPAQRERFEAHIEAARATGLPLIVHTRDAEDDTAEILGRAAGQGGVGGVLHCFTGS
                     LDLARKALDFGFYVSLSGIVTFKNARDLQETAKRIPLDRLLVETDSPFLAPVPHRGRT
                     CEPAFVADTAAFVAGLRGEEPEALAEATTANFFRLFARAAG"
     gene            3888..4652
                     /locus_tag="VFU20_04335"
     CDS             3888..4652
                     /locus_tag="VFU20_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5285726.1"
                     /translation="MKVRILGCGTSTGVPKIGNDWGRCDPHEPRNRRTRSSILLDSGG
                     ETLLVDCGPDLRQQLLDAGIGRIEALIVTHDHADHCHGIDDLRPLAQVLGRPVPLYAR
                     RHDLERLRMRFAYAFAQAGIYPPVVEGVPLEQDLRWGDAALRFVDQPHGGITSLGMRI
                     EEGDRSIVYSIDFNELTEEMANLYQGTDVWICDCLMQRPHPTHAHLDAVLGWARDLNV
                     GQVYLTHMGNSLDYATLVRELPDWAAPAHDGLEVAL"
     gene            4677..5264
                     /locus_tag="VFU20_04340"
     CDS             4677..5264
                     /locus_tag="VFU20_04340"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HEU5285727.1"
                     /translation="MTNDLMLGGVYILMAMMLVLGSLMTRREPVAKLLTLALAWVAIF
                     AAGFVLFTFRDDFGWVVQRLRAEATGEPVAQGREIRIPMAIDGHFWIGGELNGVRVKF
                     LVDSGATMTTIGRETAERAGVQVSARPDQIVRTGNGLIRVSSGRADSLTIGPIERRDL
                     AMHVADNADLNVLGMNFLSSLQRWGVEGRWLVLVP"
     gene            5368..6126
                     /gene="mazG"
                     /locus_tag="VFU20_04345"
     CDS             5368..6126
                     /gene="mazG"
                     /locus_tag="VFU20_04345"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HEU5285728.1"
                     /translation="MTLERLVAIMRRLRDPVRGCEWDSQQSFATIAPYTIEEAYEVAD
                     AIARDDMDALADELGDLQLQVVFHSQMAEEAGLFTLDDVLGAICDKLERRHPHLFGGA
                     AETPGWEAIKASERELRPDESALAGVALALPALERAQKLQRRAARTGFDWPDSSGARA
                     KIDEELAELDAESEPAAREEELGDLLFAIVNLARFLNIDAEEALRKANRKFEARFRAI
                     EAEPGFSALSLDQMEDLWVRAKAAAKAGQAGSSA"
     gene            complement(6105..7052)
                     /gene="hflX"
                     /locus_tag="VFU20_04350"
                     /pseudo
     CDS             complement(6105..7052)
                     /gene="hflX"
                     /locus_tag="VFU20_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833274.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
     gene            7042..7342
                     /locus_tag="VFU20_04355"
                     /pseudo
     CDS             7042..7342
                     /locus_tag="VFU20_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669179.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
BASE COUNT         1098 a         2536 c         2592 g         1116 t
ORIGIN      
        1 ggagggggtc ggcaagtcga cccaggtccg ggcgctcgcc gccgcgcttc gcgagcgggg
       61 catcggggtg gtcgaaacgc gcgagccggg cggcagcgaa ggcgcggagc ggatccgcga
      121 actgcttctg accggggacg acgaccgctg gggcccccag gccgaagccc tgctgttcgc
      181 ggccgcgcgg actgaccatg tcgacaaggt gatccgcccg gcgatccagg cggggaagtg
      241 ggtgctgtcc gaccgcttcg tcgacagctc gctcgcttac cagggcggcg cccgggagct
      301 tggcatcgag gcggtgcggg cggtcaatgc gttcggcatc gccgactgtt tccctgaccg
      361 cacgctggtg ctcgcgctgg ccgaaggcgg cgcccgcgcg cgtgcccgcg acgccgagga
      421 cagcgaccgg atcggcggcc gcccggaaaa ctatcaccag aaggtcgacc tcgccttccg
      481 gctgatcgcg gcggaggatc cggagcgggt gcggatcgtc gacgcgtcgg gcgagcccgg
      541 cgaagtcacg cagcggctgc ttgacgccat cgcggacctg ctgccgtgat cgccggccac
      601 gacaggcagg tcgcccagtt cgagcaggcg tggcgggcgg gaaccttgca ccacgcgtgg
      661 ctgctcgccg ggcccaaggg cgtcggcaag gcgcatttcg cccgcgccgc ggcgacccgg
      721 gtgctggccg acgccgccgg gcacgcggtg gccctgcccg ggctggagac gcccgaggag
      781 cacccgatcg cccggctcgt cgaggccggc agccatcccg acatgcgccg gctcgagcgg
      841 ctcgagaacc cgaagaccgg cgctctggcg cgcaacatca cggtcgacca ggtgcgcggg
      901 ctcgccgagc tgttcgacct gaccccgagc atgtcgccgt ggcgcgcggt ggtgatcgac
      961 gccgccgacg acctcgagaa atcggccgcc aacgccttgt tgaagatgct cgaggagccg
     1021 ccggccaatt gcctgttcct gctcgtcagc cacgctcccg ggcggctgtt gccgacgatc
     1081 cgctcgcggt gccggaggct cgacttccag ccgctggccg atgacgccat gacgtcagtc
     1141 ttggaatcgc agctgccttc gctgagcgag gccgaacgct cgcggatcgt cgccagggcc
     1201 ggcggctcgg tcggccgggc gctggcgttc gcggcgctgg atctcgccaa gctggaggac
     1261 gccgcgctca agatcctgcg cgagggcgac ccgaccaacg cgcgccggtc ggagctggcg
     1321 caggagctcg gccgcaaggg ggcgggcgag cgctatgcgg cgttcctcga cctggtgccg
     1381 gcgctgatcg cccgccaggc gcgcgggctc gaagggccgg ggctcggccg ggcgcttgac
     1441 gcctacgagc gctcgcgcga gctcgccgcg ctcgcgccgc gcgtttcgct cgatcccgct
     1501 tcgaccgtgt tccagctggg cgggatcctg gcttcggttg tggcacgccc tgttccttga
     1561 aggcccgcgc tgcggcggct agggccgcgc tcgagatggc ggaccccttc tacatcacga
     1621 ccgcgatcag ctaccccaac ggcccgccgc atatcggcca cgcctatgag gcgatcgccg
     1681 gcgacgtgat cgcccgcttc cagcgggccc aaggccgcga cgtccgcttc cagaccggga
     1741 ccgacgagca tgggctcaag atggcccagg cggcgcgctc ggaaggcgtc gagccgcgcg
     1801 actatgcgaa gaaaatgtcg tctattttcc aagagatgtg cgacgtcctt aacgtcagct
     1861 acgaccgttt catccgcacc tccgagcccg agcaccaccg cgccagccag gcgatctggt
     1921 cggcaatgga ggcgaaaggc gacctttatc tcggccgcta cgaaggctgg tactcggtcc
     1981 gcgacgaggc tttttacgac gaaagcgagc tgacgcttgc gcaaggggcc ggggaggggg
     2041 ccgagaggct atcgccgcag ggctcgccgg tcgaatggac ggccgaggag agctggttct
     2101 tccggctgtc ggcctaccag cagagattgc tcgaccatta cgaggccaat ccgggcttca
     2161 tccggcccga aagccggcgc aacgaggtgc tgcgcttcgt cgaaggcggg ctgtccgacc
     2221 tgtcgatctc gcgcaccagc ttcgactggg gagtgccggc gccgggcgcg ccgggccatg
     2281 tgatgtacgt ctggctcgac gccctgacca attacatcac cggcctcggc tatcccgacg
     2341 agaccgagct gttcgcccgc tactggccgg ccgacgtcca tctgatcggc aaggacgtgg
     2401 tgcgcttcca cgcggtctac tggccggcgt tcctgatgtc ggccggcatc gagcttccga
     2461 aaatggtcta cggccacggc ttcctgctga gcagcggcga gaagatgtcg aaaagcgtcg
     2521 gcaacgtcgt cgacccgttc gctctcgccg accgcttcgg ggtcgactcg ctgcgctatt
     2581 tcctgcttcg cgaggtgccg ttcggccagg acgggagcta cagccccgag gcgatcgtca
     2641 accgcgtcaa cgccgagctc gccaacagct tcggcaacct cgcgcaacgc acattgtcga
     2701 tggtttacaa gaatctggca ggcgaagttc cgcagcccgg agcggcggcg gaggatgtcg
     2761 cgctgctcga tgcggtccgc agcgcctgcg agcgggacgt ccccgcggag ttcgagcggt
     2821 tcgcgttcgc ctcgggcctc gaggcgtgga tgggcgcggt gttcgcctgc aacgcttatg
     2881 tcgacgccgc cgcgccctgg gcgctgcgca agagcgatcc cgggcgcatg gccgaagtcc
     2941 tcggcaccct ggtcgaggcg atcgtgattc tggccgcggc ggtcgagccg gtgatcccgg
     3001 gttcggcgcg ccgcctgatc gatttcatcc gaaagggcgg gccgatcggc cagcccgagc
     3061 cgatcttccc gcgcctcgag ctcgaagagg cggccccgtg agcacggcgc gatgaggctg
     3121 atcgatagcc attgccacct caactacccg gggctggtcg agcgccagga cgaagtgctc
     3181 gactccgccc gcgagagggg agtggggggc ttcctcaaca tagcgacgcg ccggtcggag
     3241 tggggggaca tcatcgccac cgccgagcgg cacgcggacg tctgggcgag cgtcggggtc
     3301 catccgcacg aagccgacgc gcacccggac ctcggcgccg cggcgctggt cgagggctcg
     3361 gagcacgaca aagtcgtcgc gatcggcgag tgcgggctcg attattatta cgacaattcc
     3421 gcccggccgg cgcagcgcga gcggttcgag gcgcacatcg aggcggcgcg ggcgaccggc
     3481 ctgccgctga tcgtccacac ccgcgacgcg gaggacgaca ccgccgagat cctgggccgc
     3541 gcggctgggc agggcggggt cgggggagtg ctccactgct tcaccggcag cctcgatctg
     3601 gcccgcaagg cgctcgattt cggcttctac gtgtcgctga gcgggatcgt caccttcaag
     3661 aacgcccgcg acctccagga aacggccaag cggatcccgc tcgaccggct gctggtggag
     3721 accgattcgc cgtttctcgc cccggtcccg caccgcggcc ggacctgcga gccggcgttc
     3781 gtcgccgaca ccgcggcctt cgtcgccggg ctgcgcggcg aggagcccga agcgctcgcc
     3841 gaggcgacca ccgccaattt cttccggctg ttcgcccggg cggccgggtg aaggttcgca
     3901 tcctcggctg cggcacgtcg accggggtgc cgaaaatcgg caacgactgg ggccggtgcg
     3961 atccgcacga gccgcgcaac cggcggaccc gctcgtcgat cctgctcgac agcggcggcg
     4021 agaccttgct ggtcgattgc gggcccgacc ttcgccagca gctgctcgac gccggtatcg
     4081 gccgcatcga ggcgctgatc gtcacccacg accatgccga ccattgccac gggatcgacg
     4141 atctgcggcc gctcgcgcag gtgctgggcc ggccggtgcc gctatatgcg cgccgtcacg
     4201 acctcgagcg gctccggatg cgcttcgcct acgcgtttgc ccaggcgggc atctacccgc
     4261 cggtggtcga gggcgtgccg ctggagcagg acctgcgctg gggcgatgcg gcgctgcgct
     4321 tcgttgacca gccgcacggc ggcattacat cgcttggcat gcgcatcgag gaaggcgacc
     4381 gctcgatcgt ctattccatc gacttcaacg agttgaccga ggaaatggcc aatctctacc
     4441 agggcaccga cgtgtggatc tgcgattgcc tgatgcagcg gccgcacccg acccacgccc
     4501 acctcgacgc ggtgctcggc tgggcgcgcg acctcaacgt cggccaggtc tatctgaccc
     4561 acatgggcaa cagcctcgac tatgcgaccc tggtgcgcga gctgcccgac tgggcggcgc
     4621 cggcgcatga cgggctcgag gtggcgctgt gagcacggtt ctgtgagggc cgggcgatga
     4681 ccaacgacct gatgctcggc ggagtctaca tcctgatggc gatgatgctg gtgctgggct
     4741 cgctgatgac gcggcgcgag ccggttgcga agcttctgac cctggcgctc gcctgggtgg
     4801 cgattttcgc cgcgggcttc gtcctgttca ccttccgcga cgatttcggc tgggtcgtcc
     4861 agcgtttacg ggcggaagcc acgggcgaac cggtcgccca gggccgcgaa atccgaatcc
     4921 cgatggcgat cgacggccat ttctggatcg gcggcgaact gaacggggtg cgggtcaagt
     4981 tcctggtcga cagcggcgcg accatgacca ccatcggccg cgagaccgcc gagcgcgccg
     5041 gggtccaggt cagcgcccgg ccggaccaga tcgtccgcac cggcaacggg ctgatccggg
     5101 tgtcgagcgg ccgggcggac agcctgacca tcggcccgat cgagcggcgc gaccttgcga
     5161 tgcacgtcgc cgacaacgcc gacctcaacg tgctggggat gaatttcctg tcgtcgctgc
     5221 agcggtgggg ggtcgaggga cgctggctgg tgctggtccc ctgatgcggc tttcatttta
     5281 cataatacat attatcagac tttcgggcgg agggtctaga aagggccctc gcggcgattt
     5341 tctcctcccc ccgcaaaggc ttaccccatg accctggagc ggctcgtggc gatcatgcgg
     5401 cggctgcgcg accccgtcag gggctgcgaa tgggacagcc agcagagctt cgcgaccatc
     5461 gccccttaca ccatcgagga agcctatgag gtcgccgacg cgatcgcgcg cgacgacatg
     5521 gacgcgctcg ccgacgagct cggcgatctc cagctccagg tcgtgttcca ctcgcaaatg
     5581 gccgaggaag ccgggctttt cacgctcgac gacgtgctcg gcgcgatctg cgacaagctc
     5641 gagcgccgcc acccgcattt gttcggcgga gcggccgaaa cgcccggctg ggaggcgatc
     5701 aaggcgtccg agcgcgagct gaggcccgac gagagcgccc tggcgggcgt cgctttggcg
     5761 ttgccggcgc tcgagcgcgc gcagaagctg cagcgccgcg cggcgcggac cggcttcgac
     5821 tggccggatt cgtccggggc gcgggccaag atcgacgagg agctcgccga gctggacgcc
     5881 gagagcgagc ccgcggcccg cgaggaggag ctcggcgacc tgctgttcgc gatcgtcaac
     5941 ctcgcacgct ttctgaatat cgatgcggaa gaggctttgc gcaaagccaa tcgcaagttc
     6001 gaggcccggt tcagggcgat cgaggcggag ccggggttct cggccctgtc gctcgaccag
     6061 atggaggatt tgtgggtgcg cgccaaggcg gccgccaagg ccggtcaggc cggctcgagc
     6121 gcctgaaagc gggcccaatt gtcgttcgac aggcgcaccg acagatggat ctggccgtcg
     6181 tcgccgagcg tctggttgag cacctcgccg cgggcgtgca gccaggcgat cttgctgccc
     6241 tcggcggcgg gaagccggat ctcgacgatc cggtcgtcgt gccggagcgc ctcgccgatg
     6301 cgtttctgca aggtctccag cccccagccg gtcagcgccg agatcggcac gacgtcgtca
     6361 cggcgctcgg cttcgttgag cagcgcctgg cgctcgtcgc cgccgagcag gtcgagcttg
     6421 ttccatgcct cgagccgcgg cggcgccccc tcctccccct ctgcgcccag gcccagggag
     6481 gccagcacgt cctcgacgtc gtcgcgctgc gcctcgtggt cggggtgcgc catgtcgcgg
     6541 acgtggacca gcaggtcagc cgacgcgacc tcctcgaggg tcgcccggaa cgccgccacc
     6601 agctcggtcg gcaaatccga gacgaagccg accgtgtccg acaggatcgc cttgtcgaag
     6661 ccggggatcc ggatttcgcg catggtcggg tcgagggtcg cgaacagaag gtcctcggcg
     6721 aacaccgcct cgcccgtcag gcggttgaaa agcgtcgatt tcccggcgtt ggtgtagccg
     6781 accagggcga ccaccggcca cggcgcgcgc tggcgccggt cgcggtgcag ggtgcgggtg
     6841 cgcctgaccg ccaagagctc gcggcgaatc cgcgccatgc gctcgcggat caggcgccgg
     6901 tcggcctcga tctgggtctc gcccgggccg ccgaggaaac cgaagccgcc gcgctggcgc
     6961 tcgaggtggg tccagctgcg caccagccgc ccggcctgat agtcgagatg ggcgagctcg
     7021 acctgcagcc ggccctccga ggtcatcgcc ctggtcggct acaccaacgc cggcaagtcg
     7081 accctgttca accggctgac gggctccgag gtcttggcca aggacctgct gttcgccacc
     7141 ctcgacacca cccagcgcac catccgcctg ccgcagggcc gcccggcgat catcgccgac
     7201 accgtgggct tcatctccga cctgccgcac gaactggtcg agagcttccg cgccacgctg
     7261 gaagaggtgg gcgaggccga cctgatcctg cacgtccgcg acatcgcctc ggctgactcg
     7321 gccgcccagg ccagggacgt cg
//
LOCUS       DASTZO010000154         4947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_874665,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000154 DASTZO010000000
VERSION     DASTZO010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874665"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..494
                     /locus_tag="VFU20_04360"
     CDS             <1..494
                     /locus_tag="VFU20_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5285729.1"
                     /translation="IISDEARLQLTVRSYTPEVRKLLLDGIKRIARGEAIAAGMPEDR
                     MPVVTIREEMYTPATYNTEKFSNRALELFTGHFGKERAIKTPAVMGGEDFSRFWLADK
                     SIESLIFWVGGTPIDKWKAAGGDPQKLPSLHSPFWAPQADAVIATATEAMTLAALDVL
                     RKG"
     gene            complement(491..1426)
                     /locus_tag="VFU20_04365"
     CDS             complement(491..1426)
                     /locus_tag="VFU20_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014849031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5285730.1"
                     /translation="MEKRRIGDSALEIAPLALGGNVFGWTADEPASFAILDAFVDAGG
                     TMIDTADVYSAWAPGHCGGESETVIGRWLERDPGKRGKVVIATKVGMMAGLAPERVAA
                     ACDGSLQRLGVETIDLYYQHKDDEAVPLADSLGAFDELKRAGKIRAVGLSNFAPDRLD
                     EALATAAEGGLTPPVAYQGWYNLVEREKFEGPLRDTVVGAGLAMFPYYSLANGFLTGK
                     YRSEADLAKSVRGGRNREYLEGRGPRVLAALDTVAAETGAALATIALAWTMARPGVTA
                     PIAGANSLAQVKEIVAALSFELTPAQVERLNAASA"
     gene            1546..3603
                     /gene="prsK"
                     /locus_tag="VFU20_04370"
     CDS             1546..3603
                     /gene="prsK"
                     /locus_tag="VFU20_04370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA/PEP-CTERM system histidine kinase PrsK"
                     /protein_id="HEU5285731.1"
                     /translation="MDALIPFWSYALASAMFVALLVWRIGAGASQPGHRLLLGAFAMT
                     GCWAWLYAIGSGSLMVSYAETARNLLWISLLYSLSATGGARQHGVRLVYGAVSAVFGL
                     QLVADTLALFVQSGALLQTATLLRITAAAGSLVLVHNLYGQAAPASRPTIRFVMLGLA
                     LIWVYDLNLYTILYLDLATASELLDWRGAAVALTGPLFALAAKQEDGWRIRLSRAATF
                     QSLSLLAICAYFAVMTVLATALRGSGWDWSGNLLVALLAAMTVGAMVLLPSPSARSWA
                     KVKLAKHLFEHRYDYRTEWLRFTDTLGRTGTDAPPLAERVIKAFADIVDAPGGLLLVK
                     DSAGAVTTAASWNWPGHNPQAGDLETAGGFWNDVESSGRILELDAYRRRWGHPKDLVI
                     AVPRWIADDDCAWAGVPLIHNDRLFGLILLAAPDYRRALDWEDFDLLRTAGRQAASSL
                     AEAHGQEALSNAQRFDEFNRRFAFILHDIKNLVSQLSLLSRNAERHADNQEFRSDMIA
                     TLKSSVGKMNELLARLSPQAAQRAQRSEPTPLRAILSSAIAAKRRNHEVRLLGDSSLR
                     AVVDATGLEQALGHLLQNAVEASPPGKPVTVRVGQQGGAVLVRITDQGCGMDGDFIRN
                     RLFQPFASTKPDGFGIGSFEARALIAAMGGRLSVDSRPGHGTTFTIMLAADPAGAASR
                     KRA"
     gene            3600..>4947
                     /gene="prsR"
                     /locus_tag="VFU20_04375"
     CDS             3600..>4947
                     /gene="prsR"
                     /locus_tag="VFU20_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM-box response regulator transcription
                     factor"
                     /protein_id="HEU5285732.1"
                     /translation="MSTSPDLPVLLVVEDDEGLQRQLKWAYDGYRVVVAGDRASAVEA
                     LRQHEPAVVTLDLGLPPDPDGTEEGFATLTEMVRLKPDTKVIVASGHGARESALKAIA
                     CGAYDFYRKPVDIDELGLIVARAFHLHGIEAENRRLEANGDSAVLGSMISAAPEMLKV
                     AKTIERVATAGVSVMLLGASGTGKELLARAVHEKSGRSGGAFIAINCAAIPENLLESE
                     LFGYERGAFTGAVKANVGKIELAEGGTLFLDEVGDIPLALQVKLLRFLQERVIERIGG
                     RQPIAVDTRIVCATHQDLQAMIGEGRFREDLYYRLAEVVVKIPSLSERAGDSVLLARH
                     FVNLFAREMNPKVQGLSPDAVAAIDAYPWPGNVRELENRIKRAVIMTDGRLVGAEDLD
                     LPCGTEEAPATINLRAAREVADRRAIRQAMSRTDNNISSAAKLLGISRPTLYDLLKQ"
BASE COUNT          755 a         1778 c         1663 g          751 t
ORIGIN      
        1 acatcatctc tgacgaggcg aggctccagc tgacggtgcg cagctacacg cccgaagtcc
       61 gcaagctgct gctcgacggg atcaagcgca tcgcccgcgg cgaggcgatc gcggccggca
      121 tgcccgagga ccggatgccg gtcgtcacca tccgcgagga aatgtacacg cccgccacct
      181 acaacaccga gaaattctcc aaccgcgcgc tcgagctgtt caccgggcat ttcggcaagg
      241 aacgcgcgat caagaccccg gcggtgatgg gcggcgagga tttcagccgc ttctggctcg
      301 ccgacaagtc gatcgagagc ctgatcttct gggtcggcgg caccccgatc gacaagtgga
      361 aggcggccgg cggcgacccg cagaagctgc cgtcgctgca cagcccgttc tgggcgccgc
      421 aggccgatgc ggtgatcgcg accgcgaccg aggcgatgac cctggccgcg ctcgacgtgc
      481 tcaggaaggg ctaggcgctc gccgcgttga gccgctcgac ctgggcggga gtcagctcga
      541 agctcaatgc ggcgacgatc tccttgacct gggcgagcga gtttgcaccc gcgatcggcg
      601 cggtcacgcc cggccgggcc atcgtccagg cgagcgcgat ggtggcgagc gccgcgccgg
      661 tctcggccgc gaccgtatcg agcgcggcga ggacgcgcgg tccccgcccc tccagatatt
      721 cgcggttgcg gccgccgcgg acgcttttcg ccaggtcggc ctctgagcgg tacttgccgg
      781 taaggaagcc gttggcgagg ctgtaataag ggaacatcgc caggcccgcg ccgaccaccg
      841 tatcgcgcaa tggaccctcg aatttctcgc gctcgaccag gttgtaccag ccctggtagg
      901 cgaccggcgg cgtcagcccg ccctcggcgg cggtagcaag cgcctcgtcc agccggtccg
      961 gcgcgaagtt cgacaagccg accgctcgaa tcttgccggc ccgcttcagt tcgtcgaacg
     1021 cgccgaggct gtcggcgagc ggcaccgcct cgtcgtcctt gtgctggtag taaaggtcga
     1081 ttgtctcgac gccgagccgc tgcagcgacc cgtcgcacgc ggccgcgacc ctctccggcg
     1141 ccagcccggc catcatcccg accttggtcg cgatcaccac cttgccgcgc ttgcccggat
     1201 cgcgctcgag ccagcggccg atcacggtct cgctctcgcc gccgcagtgg ccgggcgccc
     1261 aggcggaata gacatcggcg gtgtcgatca tcgtcccgcc ggcgtcgacg aacgcgtcga
     1321 ggatggcgaa gctcgccggc tcgtcggcgg tccagccgaa cacgttgccg ccgagcgcca
     1381 gcggcgcgat ctccagcgcg ctgtcgccga tccgccgctt ttccatctcg cgcacctccg
     1441 cgatcccaat ggcggaaaga cgccgtttcc ggaagcgttc agcatttctt tgtaaagttt
     1501 agcgacactc gcggccatgt cggacgagtt gacagtcggg ccgccgtgga cgccctgatc
     1561 cccttctgga gctatgcgct cgcgtcggcg atgttcgtcg cgctgctcgt ctggcgcatc
     1621 ggcgccggcg ccagccagcc ggggcaccgc ttgctgctcg gagcattcgc gatgaccggc
     1681 tgctgggcct ggctgtacgc gatcggctcc ggcagcctga tggtcagcta cgccgagacc
     1741 gcgcgcaacc tgctgtggat ctcgctgctc tacagcctgt cggcgaccgg cggcgctcgc
     1801 cagcacggcg tccggctggt ctatggcgcg gtatcggcgg tgttcgggct gcagctggtc
     1861 gccgacacgc tggccctgtt cgtccaaagc ggcgcactgc tccagaccgc gaccctgctt
     1921 cggatcaccg cggccgccgg ctcgctggtg ctggtccaca acctctacgg ccaggccgcg
     1981 cccgcgagcc ggccgacgat ccgcttcgtc atgctcgggc tggcgctgat ctgggtctac
     2041 gacctcaacc tctacaccat cctctatctc gacctggcga ccgcctcgga gctgctcgac
     2101 tggcgcgggg ccgcggtggc cctgaccggc cccttgttcg cgctcgccgc caagcaggag
     2161 gacggctggc ggatccggct gtcgcgggcg gcgaccttcc agtcgctctc gctgctggcg
     2221 atctgcgcct atttcgcggt gatgacggtc ctggcgaccg cgcttcgcgg ctcgggctgg
     2281 gactggtcgg gcaacctgct ggtcgcgctg ctggcggcga tgacggtcgg agcgatggtg
     2341 cttctgccga gccccagcgc gcgcagctgg gccaaggtca agctcgccaa gcatctgttc
     2401 gagcaccgct acgactatcg caccgaatgg ctgcgcttca ccgacaccct ggggcgcacc
     2461 gggaccgacg ctccgccgct cgccgagcgg gtgatcaagg cgttcgccga catcgtcgac
     2521 gcgcccggcg gtctgctgct ggtcaaggat tcggccggcg ccgtcaccac cgccgcatcc
     2581 tggaactggc cgggccacaa cccgcaggcc ggcgacctcg agacggccgg gggcttctgg
     2641 aacgatgtcg aaagctcggg ccggatcctc gagctcgacg cctaccggcg ccgctggggc
     2701 catcccaagg acctcgtcat cgccgtcccg cgatggatcg ccgatgacga ttgcgcctgg
     2761 gccggagtgc cgctgatcca caacgaccgc ttgttcggcc tgatcctgct cgccgcgccc
     2821 gattaccggc gggcgctcga ctgggaggac ttcgacctgc tgcgcaccgc cggtcggcag
     2881 gcggcgagct cgctggccga ggcccacggg caggaggcgc tgtccaacgc ccagcggttc
     2941 gatgaattca accgccgctt cgccttcatc ctccacgaca tcaagaacct ggtcagccaa
     3001 ttgtcgctgc tgtcgcgcaa cgccgagcgc cacgccgaca accaggagtt ccggagcgac
     3061 atgatcgcga ctttgaaaag ctcggtcggc aagatgaacg agctgctcgc gcggctgtcg
     3121 ccgcaggcgg cgcagcgggc ccagcgcagc gaaccgacgc cgctgcgcgc gatcctgtcc
     3181 tcggcaatcg ccgccaagcg ccgcaaccac gaggtccggc tgctcggcga ctcctcgctg
     3241 agggcggtcg tcgacgcgac cggtctcgag caggcgctcg gccatctgct ccagaacgcg
     3301 gtcgaagcca gcccgccggg caagccggtc accgtccggg tcggccagca gggcggcgcg
     3361 gtgctggtgc ggatcaccga ccagggctgc ggaatggacg gcgacttcat ccgcaaccgg
     3421 ctcttccagc cgttcgcctc gaccaagccc gacggcttcg gcatcggctc gttcgaggcg
     3481 cgagcgctga tcgctgcgat gggcgggcgc ctcagcgtcg acagccggcc cggccacggc
     3541 accaccttca ccatcatgct cgccgccgac ccggcgggcg ccgcctcacg gaaacgcgca
     3601 tgagcacttc accggacctt cccgtgctgc tggtcgtcga ggacgacgag ggcctgcagc
     3661 ggcagctgaa atgggcctac gacggctacc gggtggtcgt cgccggcgac cgcgcatcgg
     3721 ccgtcgaggc gcttcggcag cacgagccgg cggtcgttac gctcgacctc ggcctgccgc
     3781 ccgaccccga cgggaccgag gagggcttcg ccacgctgac cgagatggtc cgcctgaagc
     3841 ccgacaccaa ggtcatcgtc gcgtccggcc acggcgcccg cgagagcgcc ctgaaggcga
     3901 tcgcctgcgg cgcctacgat ttctaccgca agccggtcga catcgacgag ctcgggctga
     3961 tcgtcgcccg agccttccat ctgcacggca tcgaggccga gaaccggcgc ctcgaggcga
     4021 acggggactc ggcggtgctc gggtcgatga tcagcgccgc gcccgagatg ctcaaggtcg
     4081 ccaagaccat cgagcgcgtg gccacggccg gagtctcggt gatgctgctg ggagcgagcg
     4141 gcaccggcaa ggaattgctc gcccgcgcgg tgcacgagaa aagcggccgc agcggcggcg
     4201 ccttcatcgc catcaattgc gcggcgattc ccgagaacct gctcgaatcc gagctgttcg
     4261 gctacgagcg cggggcgttc accggcgcgg tcaaggccaa tgtcggcaag atcgagctcg
     4321 ccgagggcgg caccctgttc ctcgacgagg tcggcgacat cccgctggcg ctccaggtca
     4381 agctactgcg cttcctccag gaacgggtga tcgagcgcat cggcggccgc cagccgatcg
     4441 cggtcgacac gcgcatcgtc tgcgcgaccc accaggacct gcaggcgatg atcggcgagg
     4501 gccgattccg cgaggacctc tattaccggc tcgccgaggt ggtggtgaag atcccgtcgc
     4561 tgtccgagcg cgccggcgat tcggtcctgc tcgcgcgcca tttcgtcaac ctgttcgcgc
     4621 gcgagatgaa ccccaaggtg cagggcctgt caccggacgc ggtggcggcg atcgacgcct
     4681 atccgtggcc gggcaacgtc cgcgagctcg agaaccgaat caagcgcgcg gtgatcatga
     4741 ccgacggcag gctggtcggc gccgaggacc tcgacctgcc atgcggaacg gaggaagcgc
     4801 cggcgaccat caatttgcgc gccgcgcgcg aggtcgccga ccgccgcgcc atccgccagg
     4861 cgatgagccg caccgacaac aatatctcca gcgccgccaa gctgctcggg atcagccggc
     4921 cgacgctcta cgacctgctc aagcagt
//
LOCUS       DASTZO010000155         3200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_350119,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000155 DASTZO010000000
VERSION     DASTZO010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350119"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="VFU20_04380"
     CDS             <1..308
                     /locus_tag="VFU20_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidohydrolase family
                     protein"
                     /protein_id="HEU5285733.1"
                     /translation="SLAGVDPALIERHGAVSPEVAVALADGASSRLAADIGIGITGIA
                     GPGGGTPSKPVGTVVFARAERDADPARIVADQKVFEDNGRSGVRLQAALCALELLMP"
     gene            complement(269..730)
                     /locus_tag="VFU20_04385"
     CDS             complement(269..730)
                     /locus_tag="VFU20_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HEU5285734.1"
                     /translation="MPRHSETKHLPYTPDQLFDLVADVGSYDEFLPWVVAVRVRSSSE
                     TETVADLVVGFNAFKERFTSRVTKQRPTRICVDYIDGPLKYLKNEWRFEAAPGGGTEL
                     FFSVDFAFRSRLFEALAGQMFDRALRRMTGAFEQRAAALYGISSSRAQSAA"
     gene            complement(730..1662)
                     /gene="lipA"
                     /locus_tag="VFU20_04390"
     CDS             complement(730..1662)
                     /gene="lipA"
                     /locus_tag="VFU20_04390"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU5285735.1"
                     /translation="MTDPAPIAQHGRARQRKPDWIRVKAPTSPGYHETRRLMRSLGLV
                     TVCEEAACPNIGECWTKRHATVMILGDTCTRACAFCNVKTGMPGAVDPLEPEHVAVAA
                     AELGLEHIVVTSVDRDDLADGGASQFVKVIEALRKRAPATTIEILTPDFRNKGEAPIE
                     AIVEAGPDVFNHNLETVPRLYPTIRPGARYYASLRLLESVKRRAPHIFTKSGLMVGLG
                     EERLEVHQVMDDMRAAGIDFLTMGQYLQPTPRHAKVAEFVSPKAFDAYAAIARAKGFL
                     LVAATPLTRSSYHAGDDFRAMQEARRAQLERISG"
     gene            1730..2365
                     /locus_tag="VFU20_04395"
     CDS             1730..2365
                     /locus_tag="VFU20_04395"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEU5285736.1"
                     /translation="MTRFSDLIEGYQRFRATSWPSERQRWAELAEGQSPKVMVIACSD
                     SRVEPAVIFDARPGEMFVVRNVANLAPPFETTGGHHGVSAALEFAVTQLEVEEILVLG
                     HGSCGGCAAALTGQFDDADHGEGHFIAAWVDLLKDARAKVRSHHPQLDREAFLAMELE
                     AVKVSLANLRTFPWVIERESAGRLALHGGHFSIAEGRLYLLDEAENTFRPA"
     gene            complement(2434..2766)
                     /locus_tag="VFU20_04400"
     CDS             complement(2434..2766)
                     /locus_tag="VFU20_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285737.1"
                     /translation="MKKYLGITAAMAASVAVTGCATNPYGYQDPYAQQQAGRAATGAA
                     AGAVGGAIAGAVIPGVSTTEGAIAGAVLGGVLGAVIKGRQYYRDTRGYCYYVDQYGRP
                     IYDYNTRC"
     gene            2934..>3200
                     /locus_tag="VFU20_04405"
     CDS             2934..>3200
                     /locus_tag="VFU20_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU5285738.1"
                     /translation="MSDRNWPERNSDAEKNIALLIDADNASPEHLDEVLLVLGELGTI
                     NVRRAYGNWAKASLKGWEKLTGLHSIIPMQQFDVAKGKGATDMRL"
BASE COUNT          486 a         1071 c         1120 g          523 t
ORIGIN      
        1 tctcgctggc gggcgtggac cctgcgctga tcgagcgcca cggggcggtg tcgcccgagg
       61 tggcggtggc gctcgcggac ggggcgagtt cgcggctggc ggcggatatc gggatcggca
      121 tcaccgggat cgccgggccg ggcggcggca ctccctccaa gccggtcggg accgtcgtct
      181 tcgcccgcgc cgagcgcgac gccgacccgg ccaggatcgt cgccgaccag aaggtgttcg
      241 aggacaacgg ccgctccgga gtccggcttc aggcggcgct ttgcgccctc gagctgctga
      301 tgccgtagag cgccgcggcg cgctgctcga aggccccggt catccgcctc agggcgcggt
      361 cgaacatctg cccggcgagc gcctcgaaca gccgcgaacg gaacgcgaaa tcgaccgaga
      421 agaagagctc ggtgccaccg cccggtgcgg cctcgaagcg ccactcgttc ttcagatatt
      481 tgagcggccc gtcgatgtaa tcgacgcaga tgcgggtcgg gcgctgcttg gtcacccggc
      541 tggtgaagcg ctccttgaag gcgttgaagc cgacgaccag gtcggcgacc gtctcggtct
      601 cgctcgacga gcggacccgc accgcgacca cccacggcag gaactcgtcg tagctcccga
      661 cgtcggcgac gaggtcgaac aattggtcgg gcgtgtacgg gagatgcttc gtttcgctgt
      721 ggcgcggcat cagccggaaa tccgctccag ttgcgcgcgc cgcgcctcct gcatcgcccg
      781 gaaatcgtcg ccggcgtggt agctcgagcg ggtcagcggc gttgccgcga ccagcaggaa
      841 gcccttggcc cgcgcgatcg ccgcataggc gtcgaacgcc ttggggctga cgaactcggc
      901 gaccttggcg tggcgcgggg tcggctgcag atattgcccc atggtcagga aatcgatccc
      961 ggcggcccgc atgtcgtcca tcacctggtg gacctcgagc cgctcctcgc cgagcccgac
     1021 catcagcccc gacttggtga agatgtgcgg cgccctgcgc ttgacgctct cgagcaggcg
     1081 cagcgacgca tagtagcgcg cgcccgggcg gatcgtcgga tagagccgcg gcacagtctc
     1141 gagattgtgg ttgaagacgt cggggccggc ctcgacgatc gcctcgatcg gcgcctcgcc
     1201 cttgttgcgg aagtcggggg tcaggatctc gatggtggtc gccggggcgc gcttgcgcag
     1261 cgcctcgatg accttgacga actgcgacgc gccgccgtcg gcaaggtcgt cgcggtcgac
     1321 cgaggtgacg acgatatgct cgaggccgag ctcggccgcg gcgaccgcga catgctcggg
     1381 ctcgagcggg tcgacggcgc ccggcatgcc ggtcttgacg ttgcagaagg cgcaggcgcg
     1441 ggtgcaggtg tcgccgagga tcatcaccgt cgcgtggcgc ttggtccagc actcgccgat
     1501 gttcgggcag gcggcctcct cgcaaaccgt gacgaggccc agcgagcgca tcagccggcg
     1561 agtctcgtgg tagccagggc tggtcggcgc cttgacccgg atccaatcgg gcttgcgctg
     1621 gcgcgctcgg ccgtgttggg caatgggggc cggatcggtc atggcggcca gatagcgact
     1681 ggcgccgccg cttgccagcc ctccccgagc cgttctactc gcgccggcga tgacccgatt
     1741 ttccgacctg atcgaggggt accagcgctt ccgggcgacg tcctggccca gcgagcggca
     1801 gcgctgggcc gagcttgcgg agggccagag ccccaaggtg atggtgatcg cctgctccga
     1861 cagccgggtc gagccggcgg tgatcttcga cgcccggccc ggtgagatgt tcgtcgtccg
     1921 caacgtcgcc aacctcgccc cgccgttcga gacgaccggc ggccaccacg gagtctcggc
     1981 ggcgctggag ttcgcggtca cccagctcga ggtcgaggag atcttggtcc tcggccacgg
     2041 ctcgtgcggc ggctgcgcgg cggcgctgac cggccagttc gacgatgccg accatggcga
     2101 gggccatttc atcgccgcct gggtggatct gttgaaggat gctcgggcca aggtgcgctc
     2161 gcatcatccg cagctggacc gcgaggcgtt cctggcgatg gagctcgagg cggtgaaggt
     2221 gtcgctcgcc aatctgcgca ccttcccgtg ggtgatcgag cgcgagtcgg ccggccgact
     2281 ggctctccac ggcggccatt tttcgatcgc ggagggacgg ttgtacctgc tcgacgaggc
     2341 ggaaaataca ttccgccccg cctagcagct caacgaccgg cgacgccgcg actaacagcg
     2401 gcacccgcgc cgcctacgtc gcctgaccgg cgactagcag cgggtgttat agtcgtagat
     2461 cggccgtccg tactggtcga cgtaatagca atagccgcgc gtatcgcgat aatattggcg
     2521 gcccttgatc accgcgccga gaacgccgcc gagcaccgct ccggcgatcg cgccttcggt
     2581 ggtgctgacg cccgggatca ccgctccggc gatcgctccg ccgaccgcgc ccgcggcggc
     2641 gccggtcgcg gcgcgcccgg cctgctgctg agcgtatgga tcctggtatc cgtacgggtt
     2701 ggtggcgcag ccggtcaccg cgacgctcgc ggccatcgct gctgtaattc ctagatattt
     2761 cttcattgcc tgtcccttcc gaaactcgcc ccctgtggcc ggttcagtgc ttgagaaggc
     2821 gtttcgttcc cgcaccgatc atctgccgtt caggtccggg atcgatccgc gccgagatgt
     2881 ggtcattccg cgacactgcg ctagagggaa aaaaagacga ggattcgccc gccatgtccg
     2941 atcgcaattg gcccgaacgc aactccgacg cggagaagaa catcgcgttg ctgatcgacg
     3001 ccgacaacgc ctcgcccgag catctcgacg aagtgctgct ggtgctgggc gagctgggca
     3061 cgatcaacgt ccggcgcgcc tacggcaatt gggccaaggc gagcctcaag ggctgggaga
     3121 aattgaccgg cctgcactcg atcatcccga tgcagcagtt cgacgtcgcc aagggtaagg
     3181 gcgcgaccga catgcggctg
//
LOCUS       DASTZO010000156         3460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_718208,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000156 DASTZO010000000
VERSION     DASTZO010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_718208"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            477..683
                     /locus_tag="VFU20_04410"
     CDS             477..683
                     /locus_tag="VFU20_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285739.1"
                     /translation="MLPVVDAVPPPPVVEAPPPPAALPPVEPVAGPAFSPLLLALAGI
                     AAAVLAYYLLKDDDEDGDFDLSPG"
     gene            928..1338
                     /locus_tag="VFU20_04415"
     CDS             928..1338
                     /locus_tag="VFU20_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285740.1"
                     /translation="MALCTSSTGAIAASAQQHPVPQPSISPLVALSALGSDASRAALC
                     GASAAAAAGAAAAAQGAAQGCVLPIMDAAPPPPPPVAETMPPPPVPPPPVAPVGGGFA
                     ISPLLLALAGIAAAVLLATLLGKDKDDDDEESPA"
     gene            complement(1519..1908)
                     /locus_tag="VFU20_04420"
     CDS             complement(1519..1908)
                     /locus_tag="VFU20_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285741.1"
                     /translation="MRKGVVASLFYGAVLLGAPLTAAYSQWTPGSELVGQSVQVETNG
                     VVNTVYFDPGGAARIITPAGNVVQGNWTAAGGQLCLQTATGRECWPYNAAFQAGQQVT
                     LVSSCQVASRWTALSTNPVQATGGERG"
     gene            2066..2770
                     /locus_tag="VFU20_04425"
     CDS             2066..2770
                     /locus_tag="VFU20_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU5285742.1"
                     /translation="MILVRPQLGQNIGKAARAMLNFGLTELRLVSPRDGWPNPDAGPA
                     ASGADVILERAQLFATVAEAIADCSSVYASTVRRRDLVMPVLSPAEMADAIAASTGRS
                     AMLFGPERAGLETDEVALANAIVTVPINPEFGSLNLAQAVILLAYEWSKKSALAQPPV
                     RDLEPPAPHGELDALVAQLEDALEAKEYYHPPERTRTTKNTIRSIFTKTGWSSREVKA
                     VRGIIRALAQPRRLRG"
     gene            2864..3424
                     /locus_tag="VFU20_04430"
     CDS             2864..3424
                     /locus_tag="VFU20_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285743.1"
                     /translation="MFDMLLLALAAAAQDNGIILTTDPPHGPMPGENRTLSQSHPNAT
                     DRMFPNLAVNGLRIDGDTLYVLVANQGKARTRAPIRLFALAESNGARAEAAPVRIARL
                     SAGQSRWVPVRNFALKSAAAGRSPTVALEDASFVSASVRLSPAIPAAVDRTGQACLPG
                     RGCVLELDEANNSVRVDGRSIPRGRP"
BASE COUNT          584 a         1206 c         1071 g          599 t
ORIGIN      
        1 cgtccttccg aagcgctttg ccaaccctcg cgaatcaaag ttagctgatt ctgtggagcg
       61 ctatagcgag gcattcaaca ctgttgcgct gcggcctcgc gccatgtttt ttattgccgc
      121 aagccacatt ttcggaaacc tctgtcgctt tggggcattg tcggcccgaa ggttttccct
      181 taacaggcgg accggattga gctaagcggc caatcgatgt gaacaggagg cgttcaatgc
      241 aatcggcgag tatttccaag aagttcgcgg gggctattgc cgcgctggct ctctctacaa
      301 gttcaacagg cgcgattgcc gcctctactc catctgctgc gaaccactcg agcatcagcc
      361 cgctggtagc actgagcgca ttcggatccg acgcttcccg cgcggcgctt tgcggtgcct
      421 ctgcggttgc cgctgccggc gccgctgcag ccgctcaggg tgcagccccg gggtgcgtgc
      481 tgccggtcgt cgacgccgtg ccgcctccgc cggtggttga ggccccgcca ccacccgctg
      541 ccctgccgcc ggttgagccg gttgcaggac ctgcgttctc gccgttgctg ctggcactgg
      601 ccggaatcgc cgcggccgtg ctcgcttatt atctgctcaa agacgatgac gaggacggcg
      661 atttcgacct tagccccggc taagttcgac cctgcttcga gaataacaaa agggccgcca
      721 agtgcggccc tttttcatgt ccgaaaacgg cggattagcg ggcttatcgc aggaacagtc
      781 cgccaatttg aggcattgtc ggcgggcagg ttaaccgcta acaggccgtc cgctcgggtc
      841 aggcggccgc gatgccgtgt ggtagcagga ggcatttcaa tgcaatcggc aagtctttcc
      901 aggaatttcg caggcgctct tgccgcattg gcgttgtgca ccagctcgac cggcgcgatc
      961 gctgcctcgg cccagcagca tccggtcccg cagccgagca tcagcccgct ggtcgcgctg
     1021 agcgcgctgg gctccgacgc ttcgcgcgcc gcgctgtgcg gtgcatcggc ggccgcggcc
     1081 gccggcgccg ccgccgccgc ccagggtgct gcccagggct gcgttctacc aatcatggac
     1141 gcagcgcctc cgccaccccc gccggtcgcc gagacgatgc cgccgccgcc ggtcccgccg
     1201 ccgccggtcg cgccggtcgg cggaggcttt gcgatttcgc cgttgctgct cgcactggca
     1261 ggaatcgccg ctgccgtcct tctcgccacc ctgctgggca aggacaagga cgacgacgac
     1321 gaggagagtc ccgcctagcc agggtcggtt ttgatttcac gagcccaagt caatggccgc
     1381 attcgctgcg gccattttct tgtcgacggc cggttgagat cggcggcgcg atctaaaagg
     1441 cgcgagttaa ccgcccgccc ataaagaaat ggccgcagaa gctgcggcca tttcccgatg
     1501 acttgggtcg aaaagacgct agccgcgttc cccgccggtc gcctgaactg gattggtcga
     1561 cagcgcagtc caccgcgacg caacctggca gctgctcacc agcgtaacct gctggccagc
     1621 ctggaaggcc gcattgtagg gccagcactc gcgtccggtc gcggtctgca ggcacaattg
     1681 cccgcccgcg gccgtccaat tgccctgcac gacattgccg gcgggagtga tgattcgagc
     1741 ggctccgccc ggatcgaaat agacggtgtt cacaacgccg ttcgtctcga cctgcaccga
     1801 ttgcccgacg agctcggacc ccggagtcca ttgcgagtag gcggccgtaa gcggcgcacc
     1861 gaggagaaca gccccataaa acagacttgc aacaacgccc ttcctcataa cttcctcctc
     1921 atgcgagcaa ccggaacagt cgtgataatt agcactagcg cgagactcaa ggcataaccg
     1981 tttgaccggg ctaaggttgc aaccgccaag gatcaagctg aagctgtgtt ttaggggcaa
     2041 catgatatct aaccacgccc ccgtcgtgat tctggtccgg ccgcagcttg gccagaatat
     2101 cggcaaggcc gcccgggcga tgctcaattt cggattgacc gagcttcgcc tggtcagccc
     2161 ccgcgacggc tggcccaacc ccgacgccgg gccggccgcg agcggcgccg acgtcatcct
     2221 cgagcgggcg cagctgttcg ccaccgtcgc cgaagcgatc gccgactgct ctagcgtcta
     2281 cgcatcgacc gtccgccgcc gcgacctggt gatgccggtg ctgtcgcccg ccgaaatggc
     2341 cgatgcgatc gccgcctcga ccggccgctc ggcgatgctg ttcggccccg aacgggcggg
     2401 gctcgagacc gacgaggtcg cgctcgcgaa cgcaatcgtg accgttccga tcaatcccga
     2461 attcggctcg ctcaacctgg ctcaggccgt gatcctgctc gcctacgaat ggtcgaagaa
     2521 gagcgcgctc gcccagccgc cggttcggga cctcgagccg ccggcgccgc acggcgagct
     2581 cgatgcgctg gtcgcccagc tcgaggacgc gctggaggcc aaggaatatt accatccgcc
     2641 ggagcggacc cggacgacca agaacaccat ccgctcgatc ttcaccaaga ccggctggtc
     2701 gtcgcgcgag gtgaaggcgg tgcgcgggat catccgcgca ctcgcgcaac ccaggcggct
     2761 ccgcggctag cccgttccca agccgccggg cgtcttcgaa ccagttgaaa accggcggcg
     2821 aaaggcgcat tcatcaaccg cttggtctcg aaaggaaaag atcatgttcg acatgcttct
     2881 cctggccctt gccgccgccg cgcaggacaa cgggatcatc ctcacgaccg acccaccgca
     2941 tggtccgatg ccgggcgaaa accgcacctt gtcgcagtcc catcccaacg ccaccgaccg
     3001 gatgttcccg aacctcgccg tcaacggcct gcggatcgac ggcgacacgc tctacgtgct
     3061 ggtcgccaac cagggcaaag ctcgcacccg ggcgccgatc cggctgttcg cccttgccga
     3121 gtcgaatggc gccagggccg aggcggctcc agtccgaatc gcccgcctgt cggccgggca
     3181 aagccgctgg gtcccggttc gcaatttcgc gttgaaatcg gccgcggccg ggcgatcgcc
     3241 gaccgtcgcg ctcgaggatg cgagcttcgt ttcggccagt gtcaggctgt cgccggcgat
     3301 cccggccgcg gttgaccgca ccggccaggc ctgcctcccg ggccgcggct gcgtgctcga
     3361 gctcgacgag gcgaacaaca gcgtccgggt cgacggccgc tcgattcccc gcggccgccc
     3421 gtaacggccg caaaacttga cccttcgggc gccctcggct
//
LOCUS       DASTZO010000157         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_953556,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000157 DASTZO010000000
VERSION     DASTZO010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_953556"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..750
                     /locus_tag="VFU20_04435"
     CDS             <1..750
                     /locus_tag="VFU20_04435"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5285744.1"
                     /translation="LVIDLKAGGRVVIQLAPAFAPVHVANIKALARGRWWDGAAVYRV
                     QDNYVAQWGNNEGERPLPKGVVAKPPAEYTRPLKGLGVRPLGFADPYAPAAGYALGWP
                     VAYSPRAGWANLAHCYGSVGVGRGLSPDTGTGGELYAVIGHGPRHLDRNIAVVGRVVD
                     GIERLSSLPRGTGDLGFYKQRSEDASIARARLASEIDAGERPSYQYLDIRSASFADYL
                     RLRANRKDSFYIRPAGGVDLCNAPVPVRRTS"
     gene            complement(761..1018)
                     /locus_tag="VFU20_04440"
     CDS             complement(761..1018)
                     /locus_tag="VFU20_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285745.1"
                     /translation="MLTNPSTEMRRTYDPAGRGYHAVYHEHEVNHCPGCGRTHWLIGR
                     VSAECAFCATALPLAEASMRSHVAPVVIQHKNRPQTHAWAA"
     gene            complement(1160..1465)
                     /locus_tag="VFU20_04445"
     CDS             complement(1160..1465)
                     /locus_tag="VFU20_04445"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5285746.1"
                     /translation="MIKARIAEAGDPSERRRFSRHPVDLEARVRELGETGIDARILNI
                     SESGFMAESDGRFAVGSRVWLMLPGRERANAVVKWTAGDKLGAEFAEPISIEGIRAD"
     gene            complement(1491..1796)
                     /locus_tag="VFU20_04450"
     CDS             complement(1491..1796)
                     /locus_tag="VFU20_04450"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5285747.1"
                     /translation="MAAKFSLDGAMIPRTVKRMLDERSEERFPAGSGTAVMEFRGRRH
                     VVRLVNTSPSGAMVIFPLVPHIGEQVLLQLLDRGQVAARVRWVRDGRIGINFDGRQE"
     gene            1990..3327
                     /locus_tag="VFU20_04455"
     CDS             1990..3327
                     /locus_tag="VFU20_04455"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU5285748.1"
                     /translation="MGGRLKFWVTFSALAGLPAAWAPAQVAPGAPPAARPPVAQPVAQ
                     PAARPPAIQAPAIQPPAAAAQPPAAAVPAPPVVLPPLVWHPRDAFELLAFIQQIGSEG
                     LDPADYDPAGLALALRSGNPLLLAQTATDRFNRVSGDLAFGHVPRDEREGWHIPDKDL
                     DPTRQHMLLKAALEQHRVTDALRGLLPTHPQYRDLKNALMTTPRDQLAQINRLRLNLD
                     RWRWLPRDLGQKYIIVNVPAFYVALVENGVTRRRHKAIAGAIKTPTPMISAMAKGVIL
                     NPWWEVPKSIEPEVRGKRGFVPVKGKDGKVQRWRQPPGPTNALGEMKFVMPNPQAIYL
                     HDTNARSRFNSQVRSLSHGCIRTQNIHDFAAEILADDGGEWTREKIDATLASKKTVEA
                     KFVKPVPVYIVYFSSAASTEGKIINYEDIYKRDGKVLAALLDKRPSDRTIATR"
     gene            complement(3488..>3752)
                     /locus_tag="VFU20_04460"
     CDS             complement(3488..>3752)
                     /locus_tag="VFU20_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEU5285749.1"
                     /translation="NGAYTTGDYYHGKYGLSMKLHGHDWSNSNALSRAIVIHNAWYAE
                     PDVLAQHGKLGRSQGCFAFSRKDQWPVMQKLAGGRMIYADKLA"
BASE COUNT          588 a         1352 c         1233 g          579 t
ORIGIN      
        1 ctggtcatcg acctcaaggc cggcggccgg gtcgtgatcc agctcgcccc ggccttcgcc
       61 ccggtccacg tcgccaacat caaagcgctc gcccgcggcc gctggtggga cggcgccgcg
      121 gtctaccggg tgcaggacaa ttatgtcgcg caatggggca ataacgaggg cgagcggccg
      181 ctgcccaagg gcgtcgtcgc caagccgccg gccgagtaca cccggccgtt gaaggggctg
      241 ggggtccggc cgctcggctt tgccgacccc tatgcgccgg cggccggcta cgcgctcggc
      301 tggccggtcg cctacagccc cagggccggc tgggccaacc tcgcgcactg ctacggcagc
      361 gtcggggtcg gccgcggcct gtcgcccgac accggcaccg gcggcgagct ctacgcggtg
      421 atcgggcacg ggccgcgcca cctcgaccgc aacatcgctg tggtcggccg ggtggtcgac
      481 gggatcgagc gcctgagctc gcttccgcgc ggcaccggcg acctcggctt ctacaagcag
      541 cgcagcgagg acgcgtcgat cgcccgcgcg cggctggcga gcgagatcga cgccggcgag
      601 cggccgtcgt accagtatct ggacatcagg agcgcgagct ttgccgacta tctgcggctg
      661 cgcgccaacc gcaaggacag cttctacatc cgcccggccg gcggcgtcga cctgtgcaac
      721 gcgccggtcc cggtgcggcg aacctcctga tcggcgagcc tcaggcggcc caggcgtggg
      781 tctgcgggcg gttcttgtgc tggatgacga ccggcgcgac gtgcgagcgc atgctcgcct
      841 cggccagcgg cagagcggtc gcgcagaagg cgcattccgc cgagacccgg ccgatcagcc
      901 agtgggtgcg gccgcagccc gggcagtgat tgacctcatg ctcgtggtag accgcatggt
      961 agccacggcc ggcggggtcg taggtcctgc gcatctcggt cgacgggttg gtcagcatca
     1021 cacaaccttc ctttcgaccg ctgaacgagc gaaaatggac gcggttgcat gaatttgcct
     1081 cggcccggcg cgttgccggg gcggagtgat cgccgcgcat caggtgcaac cggcccgcgc
     1141 gggaagcgcg gcggcagccc taatcggcgc ggattccctc gatcgagatc ggctcggcga
     1201 actcggcgcc gagcttgtcg ccggcggtcc atttgaccac cgcattggcg cgctcgcggc
     1261 ccgggagcat cagccagacc cgcgacccga ccgcgaaccg gccgtccgat tcggccatga
     1321 agccgctctc cgagatgttg aggatccggg cgtcgatccc ggtctcgccg agctcgcgca
     1381 cccgcgcttc gagatcgacc gggtggcgcg agaagcgccg gcgctcgctc ggatctcccg
     1441 cttcggcgat acgcgccttg atcatcgaaa aactcatctc cacgaccagg ctactcctgc
     1501 cgcccatcga aattgatgcc gatgcgtccg tcacgcaccc accgcacccg ggcggcgacc
     1561 tggccccgat cgagcagctg aagcaaaacc tgctcgccga tatgcggcac cagcggaaag
     1621 attaccatag ctcccgacgg cgaggtgttg accagccgga cgacgtgccg gcggccgcgg
     1681 aactccatca ccgcggtgcc cgagcccgcc gggaagcgtt cttcggagcg ctcgtcgagc
     1741 atgcgtttga cggtccgcgg gatcatcgcg ccatcaaggc tgaacttggc cgccacccgt
     1801 ctttcctccc accaggacaa ggggatgcta gccatcgcgt cttaaagatt ggttttcggg
     1861 cggcttacca atgttggccg ctgtgtcccc ccagtcactg ttagccgccg ttcatcgcgt
     1921 gtatgggtcg gttgcggcat cagggcgtcg gccgagaggc tgttgtcatg ctaatataat
     1981 gggttggcta tgggcgggcg tctcaagttc tgggtaacat tctcggctct ggccgggctt
     2041 ccggccgcct gggcgccggc ccaggtcgcg cccggcgcgc cgcctgcggc ccggccgccg
     2101 gtcgcccagc cggtcgccca gccggccgcc aggccgccgg ccatccaggc accggccatc
     2161 cagccgcccg ccgccgccgc ccagccgccc gcagccgccg tcccggcgcc gcccgtcgtc
     2221 ctgccgccgc tggtctggca cccgcgcgat gcgttcgagc tgctcgcctt catccagcag
     2281 atcggcagcg aaggcctcga cccggccgat tacgaccccg ccggcctcgc tctggcgctc
     2341 cggtccggca atccgctgct gctcgcccag accgcgaccg atcgcttcaa ccgggtttcc
     2401 ggcgacctcg ccttcggcca cgttccccgc gacgagcgcg agggctggca catccccgac
     2461 aaggacctcg acccgacccg gcaacatatg ctgctcaagg ccgcgctcga gcagcaccgg
     2521 gtgacggacg cgcttcgcgg gctgcttccg acgcatccgc aataccgcga cctcaagaac
     2581 gcgctgatga ccaccccgcg cgaccagctt gcgcagatca accgcctgcg cctcaacctc
     2641 gaccgctggc gctggctgcc gcgcgacctg ggtcagaaat atatcatcgt caacgtgccg
     2701 gccttctacg tcgccttggt cgagaacggc gtcacgcgcc gccgccacaa ggcgatcgcc
     2761 ggcgcgatca agaccccgac gccgatgatc tcggcgatgg ccaagggcgt gatcctcaac
     2821 ccgtggtggg aagtgcccaa gagcatcgag cccgaggtgc gcggcaagcg cggcttcgtt
     2881 ccggtcaagg gcaaggacgg caaggtgcag cgctggcgcc agccgccggg gccgaccaac
     2941 gcgctcggcg agatgaagtt cgtgatgccc aacccgcagg cgatctacct gcacgacacc
     3001 aacgcccgga gccgcttcaa cagccaggtc cgctcgctga gccacggctg catccgcacc
     3061 cagaacatcc acgacttcgc ggccgagatc ctcgccgacg acggcggcga atggacccgc
     3121 gagaagatcg acgcgaccct cgccagcaag aagacggtcg aggcgaaatt cgtcaagccg
     3181 gtgccggtct acatcgtcta tttcagctcg gccgcctcga ccgagggcaa gatcatcaat
     3241 tacgaggaca tctacaagcg cgacggcaag gtgctcgccg cgctgctcga caagcggccg
     3301 tcggaccgga ccatcgccac ccggtagggc gcgaggcgag cgtcgccccg aatggcagag
     3361 ctcgtcacgg gagcgaatcc cgtgacgtcg gtcctgtcga ttttcgcggc ctgggccgct
     3421 cgatcgcagg ccgtccgtcg cttcgcatct ctcgctgaaa tcgtcggctg ccgctttccg
     3481 ctcggtgcta cgccaattta tccgcgtaga tcattcggcc gccggcgagc ttctgcatca
     3541 ccggccactg gtccttgcgc gagaaagcga agcagccctg gctgcggccg agcttgccgt
     3601 gctgggcgag gacgtcgggc tcggcatacc aggcgttgtg gatgacgatc gcccgcgaca
     3661 gcgcgttgct gttgctccag tcatggccgt gcagcttcat cgagaggccg tatttgccat
     3721 gataatagtc gccggtcgtg taggcgccgt tc
//
LOCUS       DASTZO010000158         4636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_350288,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000158 DASTZO010000000
VERSION     DASTZO010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_350288"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..489
                     /locus_tag="VFU20_04465"
     CDS             <1..489
                     /locus_tag="VFU20_04465"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU5285750.1"
                     /translation="DMIHAYFSPPRSMLRRLARVAARGRVRVITAGKSDIKATIGAAR
                     HSYSRMLRRGVEMYEYQPAMLHTKLAIIDDAVHIGSPNFDFRSLYINLEVSLRIESPK
                     FAAAMRGYFERERADSERITPELHRKRANAWRRFKWMVAHWLVTSMDYAVTRRLNLPL
                     ER"
     gene            complement(486..1127)
                     /locus_tag="VFU20_04470"
     CDS             complement(486..1127)
                     /locus_tag="VFU20_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5285751.1"
                     /translation="MPTTSARTHTRARRSGRRPGHRGVSPRLQFLRGFLKHPVMVGSI
                     IPSSKTVIERMLAPVDWDSTRLFVEYGPGVGTFTRPILEKLGPDATLLAIDTNPEFTE
                     FLTESLDDPRLVAVTGSAADVERLIAERGFDHADYVLSGIPFSTLPPGVGEAIAEATA
                     KVIRPGGAFLVYQFSPKVMEFIKPWFERIERGFEWLNVPPASLFWAWREPRRD"
     gene            1307..2602
                     /locus_tag="VFU20_04475"
     CDS             1307..2602
                     /locus_tag="VFU20_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5285752.1"
                     /translation="MPAPQPIRRVAATALSTPHFLLLYAAMLVAAAGNTALQSVMPAI
                     GREIGIADFWVAIAYTWSAMLWVLLAPYWAEKSDRHGRKALTLMGLAGFIVSMLLCGL
                     ALVAGLHGAIGGAVTFGLFAVFRAIYGGLGCATPSATQAYLASRTRRSGRVAALSALS
                     SSFGLGTIIGPALAPLFVLPLVGLPGPLFAFSAIGLMVYAAIWAWLPDDRLGRKTGRG
                     AAMSYPSLATTPTGASVVAATSPRLPRLKWNDARIRPWIVAGVTAGHAQAATLTCIGF
                     FVIDRLALEPRGSEGPISIVMMAGASATLAAQWGLIPRLNASPRGLILWGALIAAVGL
                     AGTMLATDLYGITIGFALASTGFGLTRPGFTGGASLAVPLAEQGAVAGVITAANGISF
                     VAAPAIGMALYAIDPHIPFAASALLLAALAIAGRRRLAA"
     gene            complement(2599..>4636)
                     /locus_tag="VFU20_04480"
     CDS             complement(2599..>4636)
                     /locus_tag="VFU20_04480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDC48 family AAA ATPase"
                     /protein_id="HEU5285753.1"
                     /translation="EIRKVQSKPATRVVFAPAQENLRLHGSAEALRRSFAARPLTEGD
                     VVATAGHLQVNVDMPEHVRQLLNAPAFALQEVRLLVVDTLPAGIVHIDSKTQIELLPE
                     YTRKDGERRADVTYDDLGGMRDTIEALREMVELPLRHPEIFQRLGVDPPKGVLLHGPP
                     GTGKTRLARAVANESEANFFHIAGPEIMGSAYGESEKRLRELFEQAAQAAPSIIFIDE
                     IDSIAPKRSQVSGEAEKRLVAQLLSLLDGLEPRQNLVVIAATNRPEALDEALRRPGRL
                     DREIVVGVPDEAGRREILAIHTRGMPLAPGVDLDELARRTYGFVGADLAALTREAALE
                     AVRRIMPRINLADEVIPTEVLDALSVELRDFDNALKRVQPSAMREVMVEAPTVGWDDI
                     GGLDDARDRLREGVELPLKHPEAFKRLGIRPARGFLLYGPPGTGKTLLAKAAARESEA
                     NFIATKSSDLLSKWYGESEQQIARLFARARQVAPTVVFFDELDSLVPARGGSFGEPQV
                     TERVVNTILAEMDGLEELHNVVVIGATNRPSLIDPALLRPGRFDELIYVGTPDEAGRA
                     RILAIHTSGMPLAKDVDLKSLAKRTERFTGADLEDLVRRAGLVALRRKLDSGDVTMAD
                     FEAALAETRASVTPEMLEEYARIQQTLKSDAVRPLGGIGFVLPGMLRPKTGEKG"
BASE COUNT          622 a         1654 c         1622 g          738 t
ORIGIN      
        1 gacatgatcc acgcctattt ctcgccgccg cgctcgatgc tgcgccggct cgcccgggtc
       61 gcggcgcgcg ggcgggtgcg ggtcatcacc gccggcaagt cggacatcaa ggcgaccatc
      121 ggcgccgccc gccacagcta cagccggatg cttcggcgcg gggtcgagat gtacgagtac
      181 cagccggcga tgctccacac caagctcgcg atcatcgacg atgcggtcca catcggctcg
      241 cccaatttcg atttccggag cctctacatc aacctcgagg tcagcctgcg gatcgagagc
      301 cccaagttcg cggccgccat gcgcggctat ttcgagcgcg agcgcgccga cagcgagcgg
      361 atcacccccg agctccaccg caagcgcgcc aacgcctggc ggcggttcaa gtggatggtc
      421 gcgcactggc tggtgacgtc gatggactat gccgtcaccc ggcgcctgaa cctgccgctg
      481 gagcgctagt cccgcctggg ctcgcgccac gcccagaaca ggctggcggg cggcacgttc
      541 agccattcga agccgcgctc gatgcgctcg aaccacggct tgatgaactc catcaccttg
      601 gggctgaact ggtagacgag gaacgccccg cccgggcgga tcaccttcgc ggtcgcctcg
      661 gcgatggcct cgccgacgcc cgggggcagg gtcgagaagg ggattcccga cagcacgtag
      721 tcggcatggt cgaagccgcg ctcggcaatg agccgctcga cgtcggcggc cgatccggtc
      781 accgccacca ggcgcgggtc gtcgagcgat tcggtgagga attcggtgaa ctcgggattg
      841 gtgtcgatcg ccagcagcgt cgcgtcgggc ccgagcttct cgaggatcgg gcgggtgaag
      901 gtgccaaccc ctgggccgta ttcgacgaac agccgggtgc tgtcccagtc gaccggcgcc
      961 agcatccgct cgatcaccgt cttgctcgac gggatgatcg atccgaccat caccggatgc
     1021 ttcaggaagc cgcgcaggaa ctgcagccgc gggctgaccc cgcgatgccc aggccggcgg
     1081 ccggagcggc gtgcacgcgt gtgggtccgg gcagacgtgg ttggcatgga agctccgact
     1141 ggggccagcg aagaggctgg cgttggcaaa tgcgccgccg cttggcaagt tccgaggccg
     1201 ccggccctag cgcggtcttc cccctgaaac gagcgctttt cgcgacgagc cctcgatagg
     1261 cggcgacggg ttccgctggc gcgggcgaga ctctaaggcg gcgcgagtgc ccgcccccca
     1321 accgatccga cgcgtcgccg cgaccgctct cagcacgccg cacttcctgc tgctctatgc
     1381 ggcgatgctg gtcgcggcgg ccggcaacac cgccctgcaa tcggtgatgc cggcgatcgg
     1441 ccgcgagatc ggcatcgccg acttctgggt cgcgatcgct tacacctggt cggccatgct
     1501 gtgggtcctg ctggcgcctt actgggccga gaagagcgac cgccacggcc gcaaggcgct
     1561 gaccctgatg gggctcgccg gcttcatcgt ctcgatgctg ctgtgcgggc tggcgctggt
     1621 cgccggactg cacggggcga tcggcggcgc ggtgaccttc ggcctgttcg cggtcttccg
     1681 cgcgatctac ggcgggctcg gctgcgcgac cccgtcggcc acccaggcct atctcgcctc
     1741 gcgcacccgg cgcagcgggc gggtcgccgc gctgtccgcc ctgtcgtcct cgttcggcct
     1801 cgggaccatc atcggcccgg cgctggcgcc tctgttcgtg cttccgctgg tcggcctgcc
     1861 gggacccttg ttcgctttct cggcgatcgg cctgatggtc tatgcggcga tctgggcgtg
     1921 gctgcccgac gaccgcctcg gccgcaagac cggccgcgga gcggcgatga gctatccctc
     1981 gctcgccacc accccgaccg gcgccagcgt cgtcgccgcg acctcgcccc ggctgccgcg
     2041 gctcaagtgg aacgatgccc ggatccggcc gtggatcgtc gccggggtga ccgccggcca
     2101 tgcccaggcg gcgaccctga cctgcatcgg ctttttcgtc atcgaccgac tggcgctcga
     2161 gccgcgggga tcggaggggc cgatctcgat cgtcatgatg gccggcgcct cggcgaccct
     2221 ggccgcgcaa tgggggctga tcccgcgcct caatgcttcg ccgcgcgggc tgatcctgtg
     2281 gggcgcgctg atcgccgcgg tcggactcgc cgggaccatg ctggcgaccg acctttacgg
     2341 aatcaccatc ggcttcgcgc tcgcctcgac cggcttcgga ctcacccgcc cgggcttcac
     2401 cggcggagcg tcgctcgcgg tcccgctcgc cgagcagggc gccgtcgccg gggtgatcac
     2461 cgccgccaac gggatcagct tcgtcgccgc cccggcgatc ggcatggcgc tctacgcgat
     2521 cgacccgcac atcccgttcg cggcctcggc gctgctgctg gccgcgctcg cgatcgccgg
     2581 ccgccgccgg ctcgcggcct agcctttctc gccggtcttc ggccggagca tcccgggcag
     2641 cacgaagccg atcccgccca gcggccgcac cgcgtcggac ttcagggtct gctggatgcg
     2701 cgcatattcc tcgagcatct cgggggtcac cgacgcccgc gtctcggcga gagcggcttc
     2761 gaaatcggcc atggtcacgt cgccggaatc gagctttcgg cgaagcgcga cgaggccggc
     2821 gcggcgcacc agatcctcga ggtcggcgcc ggtgaagcgc tcggtgcgct tggcgaggct
     2881 cttcaggtcg acgtccttgg ccagcggcat ccccgaggtg tggatggcga ggatccgcgc
     2941 ccggccggct tcgtccgggg tgccgacgta gatcagttcg tcgaaccggc cgggccggag
     3001 cagcgccggg tcgatcaggc tcggccggtt ggtcgcgccg atcaccacca cattgtggag
     3061 ctcctcgagc ccgtccatct cggcgaggat ggtgttgacc acccgctcgg tcacctgcgg
     3121 ctcgccgaag ctgccgccgc gcgccggcac caggctgtcg agctcgtcga agaacaccac
     3181 cgtcggcgcg acctgccggg cgcgggcgaa caggcgggcg atctgctgct cgctttcgcc
     3241 ataccatttg gacagcaggt cggacgattt ggtggcgatg aaattggcct cgctttcgcg
     3301 cgccgccgcc ttggccagga gcgtcttgcc ggtccccggc gggccgtaca gcaggaagcc
     3361 gcgcgccggg cggatgccga ggcgcttgaa cgcctcgggg tgcttcaacg gcagctcgac
     3421 gccctcgcgc agccggtcgc gggcgtcgtc cagtccgccg atgtcgtccc agccgacggt
     3481 cggagcctcg accatcacct cgcgcatcgc cgagggctgg acgcgcttca gcgcattgtc
     3541 gaagtcgcgc agctcgaccg acagcgcatc gagcacctcg gtcgggatga cctcgtcggc
     3601 aaggttgatc cgcggcatga tccggcggac cgcttccagc gccgcctcgc gggtcagggc
     3661 ggcgaggtcg gcgccgacga agccgtaggt tcggcgcgcg agctcgtcga ggtcgacgcc
     3721 cggcgccagc ggcatgccgc gggtgtggat cgcaaggatc tcgcgccggc ccgcctcatc
     3781 ggggaccccg acgacgatct cgcggtcgag ccggccgggg cggcgaagcg cctcgtcgag
     3841 cgcctcgggc cggttggtcg ccgcgatcac caccagattc tggcgcggct cgagcccgtc
     3901 gagcaggctc agcaattgcg cgaccagccg tttttccgcc tcgccgctga cctggctgcg
     3961 cttgggcgcg atcgagtcga tctcgtcgat gaagatgatc gacggcgccg cttgcgccgc
     4021 ctgctcgaac agctcgcgca gccgcttttc gctctcgccg taggccgagc ccatgatctc
     4081 gggcccggcg atgtggaaga aattcgcctc gctctcgttg gcgacggcgc gggcgagcct
     4141 ggtcttgccg gtgcccggcg gcccgtgcag caggacgccc ttgggcgggt cgaccccgag
     4201 gcgctggaag atctccgggt ggcgcagcgg tagctcgacc atctcgcgca acgcctcgat
     4261 ggtgtcgcgc atcccgccca agtcgtcgta ggtgacgtcg gcgcggcgct cgccgtcctt
     4321 gcgcgtatat tcggggagca gctcgatctg ggtcttggaa tcgatgtgga cgatgcccgc
     4381 gggcagagtg tcgacgacca gcagccggac ttcctggagc gcgaacgccg gcgcgttgag
     4441 caattggcgg acgtgctcgg gcatgtcgac gttgacctgc aggtggccgg cggtcgcgac
     4501 cacgtcgccc tcggtcagcg gacgggcggc gaagcttcgg cgaagcgcct cggccgagcc
     4561 gtggagccgc aaattctcct gcgccggggc gaacaccacc cgggtcgccg gcttcgactg
     4621 caccttgcgg atctcg
//
LOCUS       DASTZO010000159         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_846393,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000159 DASTZO010000000
VERSION     DASTZO010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846393"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1402
                     /locus_tag="VFU20_04485"
     CDS             146..1402
                     /locus_tag="VFU20_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285754.1"
                     /translation="MRKFRVLALAAPWVVLMIPGASGAAGHGPPAAAPRGSYLYVWAG
                     DADEKDSDFLAVIDARPGQPGYGKVVATLPVGASATMPHHVEYETPPGNVLVVNGWKA
                     GHTFLVDLANPLEPKLAGQFKSAAGYTYPHSFARLPNHNILAVFQSAGEKYAPPGGLV
                     ELDPAGRPLRSASSATADIPTDLNWPYSLAVQADLDRVVTTSTDMGMPPFEEWQTHDT
                     RHVQVWSLGDLKLLASVPLPESGKGRHHIWPAEPRVLADGTVYVNTFTCGLYRLTDLD
                     KAKPKAEFVHAFPGGTSFHDMCFVPVVMGKYWVQPVPALPGLIVLDVGDPARPVEVSR
                     FKLDARYHMPHWLAADRSSDRIVMTGDSGSWVLVLKLDPKTGTLAIDPTFRDPGAASA
                     GVNFDRAEWPHGKTGKAVVHGAVFSN"
     gene            complement(1683..2411)
                     /gene="mtgA"
                     /locus_tag="VFU20_04490"
                     /pseudo
     CDS             complement(1683..2411)
                     /gene="mtgA"
                     /locus_tag="VFU20_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457144.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
BASE COUNT          392 a          922 c          909 g          398 t
ORIGIN      
        1 aaggatatgc ttttgaaggc gttgaccatt gctgcgccca aatggttaac aaatcctttc
       61 gaacggcggt gttttgactc cgggcggcgg tgggggcagc atcccggccc tcgacggatg
      121 gccgatcggc agggggaagc gggcaatgcg caaatttcgg gttctggcgc tggcggcgcc
      181 gtgggttgta ctgatgattc cgggcgcgtc gggcgcggcc ggacacggcc cgccggccgc
      241 cgcgccgcgc ggctcgtacc tctacgtgtg ggcaggggac gccgacgaga aggacagcga
      301 cttcctcgcc gtgatcgacg cccgcccggg ccagccgggt tacggcaagg tcgtcgcgac
      361 cctgcccgtc ggcgccagcg cgaccatgcc gcatcacgtc gaatatgaaa cgccgcccgg
      421 caatgtgctg gtcgtcaatg gatggaaggc cgggcacact ttcctcgtcg acctcgccaa
      481 ccctctcgag cccaagctcg ccggtcagtt caagtcggcc gccggctaca cttacccgca
      541 cagcttcgcc cggctgccca accacaacat ccttgccgtg ttccagtcgg cgggcgagaa
      601 atatgcgcct ccgggcgggc tggtcgagct cgacccggcc ggccggccgc tgcgctcggc
      661 gtcctcggcc accgcggaca tcccgaccga tctcaactgg ccgtacagcc tcgcggttca
      721 ggcggacctc gaccgggtgg tcaccaccag caccgacatg ggcatgccgc cgttcgagga
      781 gtggcagacc catgacaccc ggcatgtgca ggtctggtcg ctcggcgacc tcaagctgct
      841 cgccagcgtt ccgcttcccg agagcggcaa gggccgccac cacatctggc ccgccgagcc
      901 gcgggtgctc gccgacggaa cggtctatgt gaacaccttc acctgcgggc tctaccggct
      961 gaccgacctc gacaaggcca agcccaaggc ggagttcgtc catgcgttcc cgggcggcac
     1021 ctcgttccac gacatgtgct tcgtgcccgt cgtgatgggc aaatactggg tccagccggt
     1081 gccggccctg ccgggcctga tcgtgctcga cgtcggcgac cccgcccggc ccgtcgaagt
     1141 ttcccggttc aagctcgacg ctcgctacca catgccgcat tggctcgccg cggaccgctc
     1201 ctcggaccgg atcgtcatga ccggcgacag cggctcctgg gtgctggttc tcaagctcga
     1261 cccgaaaacc gggacgctgg cgatcgaccc caccttccgg gacccgggcg cggcgagcgc
     1321 cggagtcaat ttcgaccggg ccgaatggcc gcacggaaag accggcaagg ccgttgtcca
     1381 cggggcggtg ttcagcaact aggaaggcga cgagctcact gccccggcgg cggtagcgga
     1441 ggctcctcgg ccggcggcgg cggttcggcg ggcggggtct cgggcggctg cgccggctcc
     1501 ccgacattgt ccgccggcgg ttctcccgga agcagcggct ggtccgatgc cggcggcagt
     1561 tcctcgacca cctcctcgag cggcggcagc ggcttggcgc tttcatattc ctcgcccggg
     1621 agcgtcctcg gcgcccggcc tggcggcggc gccttgtccc tgggcggaac gatgccacgg
     1681 tagacgcagg cgtcgagccc gtcgcgcgcg acgaccccga tgcgggccgc gatggtcccg
     1741 ccgtagcgcc gggtgaagcc cttgggggcg accgcgccgc gcttcttggg cagcggcaga
     1801 accgcggcga tgcgcgccgc ctcggtgcgg ctcagcgcgc tcgagtcatg gccgaaatag
     1861 cgctgcgccc cggcattgac gccgtaggtg ccgatgccgg tctcggcgag gttgagatag
     1921 acctccatga tccgctgctt gggccacatc gcctcggtga gcagcgtgaa ccaggcctcg
     1981 agcccttttc ggaaatagcc gccgccctgc cacaggaagg cattcttggc ggtctgctgg
     2041 ctgatcgtcg atccgccgcg gatgcgcccg cccgaggcat tgcgctgcat cgcctcctcg
     2101 atcgcgtcga agtcgaagcc attgtggctg cagaacttgc tgtcctcgcc ggcgatcgcg
     2161 gcccggacca tgtcgcggtc gatgcgctcg atcggcatcc agtcgcgctc cgcgccgcgc
     2221 ccggcgatga ggtcgccggc catggtcgcg gtcagcggcg ggttgatgaa ccggtaggcg
     2281 agcacccaca gcagcgagcc gaggatgaag atcagcacgg cccggacgac ccagcccacg
     2341 atccgccgcg gccacgagcg cgggcgccgg cgcttgtagc gcgggatgct ggactgcggg
     2401 cggcgggcca tcgccgcgcc ctagcgaagc gcggcgcaag cgtcgaggcg gcatcccggg
     2461 cgcgacattt ccaaacccgg cgctgctcgc ccgcggaaat gtaaccgccc gccgctgccg
     2521 cccgaataag agagcagtga cgtggctgag cggagaccgg cgatggcgag gaaaatcgac
     2581 gtgagggcgg ccctgtgggc ggcggccgcg gccggaatcg t
//
LOCUS       DASTZO010000160         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_457560,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000160 DASTZO010000000
VERSION     DASTZO010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457560"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1382
                     /locus_tag="VFU20_04495"
     CDS             <1..1382
                     /locus_tag="VFU20_04495"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU5285755.1"
                     /translation="KNNCTTGQIYETVIAKERRGDYLGGTVQVIPHITDEIKRRILAA
                     AEGYDIAIGEVGGTVGDIEGLPFLEAVRQIAWDWGRENVLYIHLTLVPYITGGGELKT
                     KPTQHSVRELTSLGIQPDVLLCRVDRPLPDSDRAKIALFCNVAKSAVIPALDARSIYD
                     VPLQYHAEGLDDEVLKAFGIDDAPRPDLARWHDIMDRLDNYEGEVTIGVVGKYVGLPD
                     AYKSLNEALVHGGLANRAKVNIRWLDAEMFEHEDANLAAELEPLHGILVPGGFGERGS
                     EGKIASVRFARERGVPFFGICLGMQMACIEGARNLAGISEASTTEFGDTPEPVVGLIT
                     EWMSEEGLQQRAAGGELGGTMRLGAYPARLAANSHVAAIYGATEISERHRHRYEVNVH
                     YRDALEKGGLLFSGMSPDGQLPEIVERPDHPWFLGVQFHPELKSRPFDPHPLFAGFIA
                     AALKQSRLV"
     gene            1467..1979
                     /locus_tag="VFU20_04500"
     CDS             1467..1979
                     /locus_tag="VFU20_04500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HEU5285756.1"
                     /translation="MKAFVAIPASLALVLSLAACDRAAGPAESNAAASAQGATADAQA
                     VKQAFAAFNADIAAKKLDAIRAHYASDAVMILPGQKPFEGIDSIMTDYEGYVADPASK
                     YVPGAETTEVSSDGDLAYGEVKYQTTFTNSTTKAVETSDRYNLTVYKKQQDGSWKVVR
                     DVNVALPAAG"
     gene            2072..3037
                     /gene="trxB"
                     /locus_tag="VFU20_04505"
     CDS             2072..3037
                     /gene="trxB"
                     /locus_tag="VFU20_04505"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU5285757.1"
                     /translation="MTNTVSTRVAILGSGPAGLTAAIYAARAGLAPILIQGIQPGGQL
                     TTTTDVENYPGFRDVIQGPWLMEEMQAQAEHVGTKVVWDHITAVDLSRRPFRLTGDGG
                     TAYSADTLVIATGAQAKWLGLPSEEHMKGRGASACATCDGFFYRGKKVAVIGGGNTAV
                     EEAMYLTNHSHDVTLIHRRDSLRAEKILQDRLFANPAIKVIWNHEVAEFIGGGDPEAL
                     IGLDIRDTRSGAVSRIDVEGAFVAIGHTPATELFAGQLDLDEDGYIRVQDGSTRTSVE
                     GVFACGDVMDKIYRQAVTAAGTGCMAALDAEKYLAAREFEAMAAE"
     gene            complement(3060..>3339)
                     /locus_tag="VFU20_04510"
     CDS             complement(3060..>3339)
                     /locus_tag="VFU20_04510"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF014332.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEU5285758.1"
                     /translation="ADVTNGARLETYVIPAPRGGGTITINGAAAHLVHAGDLVIIMSY
                     AVVDDSEAKRMKPLVVHVDAANRAIKLGNDPAEPAPGQAGQKRAVPLG"
BASE COUNT          578 a         1113 c         1126 g          522 t
ORIGIN      
        1 agaaaaataa ctgcactacc gggcagattt atgaaaccgt gatcgccaag gaaaggcgcg
       61 gcgattatct cggcggtacg gtgcaggtca ttccgcacat caccgatgag atcaagcggc
      121 gcattctcgc ggcggcggaa ggatacgata tcgccatcgg cgaagtcggc ggcacggtcg
      181 gcgacatcga ggggttgccg tttctcgagg cggtgcggca aatcgcctgg gattggggac
      241 gggagaatgt gctctatatc catctcactc tggtgcctta catcaccgga ggcggcgagc
      301 tcaagaccaa accgacccag cattcggtgc gcgagctgac cagcctcggg atccagcccg
      361 acgtcctgct gtgccgggtc gaccggccct tgcccgacag cgaccgggcc aagatcgctc
      421 tgttctgcaa cgtcgccaag tcggcggtga tcccggcatt ggatgcccgc tcgatctacg
      481 acgtgccgct gcaatatcat gccgagggcc tcgacgacga agtcctgaag gcgttcggga
      541 tcgacgacgc gccgcggccc gacctcgccc gctggcacga catcatggac cggctcgaca
      601 attacgaggg cgaggtgacg atcggcgtgg tcggcaaata tgtcgggctc ccggacgcat
      661 acaagagctt gaacgaagcg ctggtccacg gcgggctcgc caaccgggcc aaggtcaaca
      721 tccgctggct ggatgccgag atgttcgagc atgaggacgc caacctcgcg gccgaattgg
      781 agccgctgca cgggatcctc gtccccggcg gcttcggcga gcgcggcagc gagggcaaga
      841 tcgcctcggt ccggttcgcc cgcgagcggg gcgtgccgtt cttcggcatc tgcctcggca
      901 tgcagatggc gtgcatcgag ggcgcccgca atctcgccgg gatttccgag gcctcgacca
      961 ccgagttcgg cgacacgccc gagccggtgg tcgggctgat caccgagtgg atgagcgagg
     1021 agggccttca gcagcgcgcc gcgggcggcg agctcggcgg caccatgcgg ctgggcgcct
     1081 acccggccag gctggcggcc aacagccacg tcgccgcgat ctacggcgcg accgagatca
     1141 gcgagcgcca ccgccaccgc tacgaggtca acgtccacta tcgcgacgcg ctggagaagg
     1201 gcgggctgct gttctccgga atgtcgcccg acggccagct gcccgagatt gtcgagcggc
     1261 ccgaccaccc gtggttcctc ggcgtccagt tccacccgga gctcaagagc cgcccgttcg
     1321 acccgcatcc cctgttcgcc ggcttcatcg cggcggcgct gaagcagtcg cggctggtct
     1381 agcgtttaac atcccgtcag cgaaatgact tgccaagccg cgccaatggg tattgtgtcc
     1441 cccgcttcct gaggggggga gcaatcatga aagcttttgt cgccatcccg gccagcctgg
     1501 cgctggtcct gtcgcttgcc gcttgcgatc gcgcagcggg tcccgccgaa agcaatgccg
     1561 cggcgagcgc gcaaggggct acggccgatg cccaggcggt caagcaggcc tttgccgcct
     1621 tcaacgcgga catcgcggcc aagaagctcg acgcgatcag ggcgcactat gccagcgacg
     1681 cggtgatgat ccttcccggt caaaagccct tcgagggcat cgattcgatc atgacggact
     1741 atgagggata tgtcgccgat ccggcgagta aatatgttcc aggcgcagag acgaccgaag
     1801 tctcctcgga cggcgacctg gcttacggcg aggtgaagta ccagaccacc ttcaccaatt
     1861 cgacgaccaa ggcggtcgag acgtccgacc ggtacaatct cacggtgtac aagaaacagc
     1921 aggacggcag ctggaaggtc gttcgggacg tcaacgtggc cttacccgcg gccggctgaa
     1981 ccgcacccgg gacggagccg ccgagtgccg cggcggcccg tcctttttcg gccattgcgg
     2041 cgcctgaatg gcgcatctac atcggcccgg catgaccaac accgtctcca cccgcgtcgc
     2101 gatcctcggc tccgggcccg ccggcctcac cgccgccatc tatgcggcgc gggccgggct
     2161 cgccccgatc ctcatccagg gaatccagcc gggcggccag ctgaccacca ccaccgacgt
     2221 cgagaattac ccgggcttcc gcgacgtcat ccagggccca tggctgatgg aggagatgca
     2281 ggcccaggcc gagcatgtcg ggaccaaggt ggtgtgggac catatcaccg cggtcgacct
     2341 gtcgcgccgc ccgttccggc tgaccggcga cggcggcacc gcttattcgg ccgacacgct
     2401 ggtgatcgcg accggcgccc aggcgaagtg gctcggcctt ccgtccgagg agcatatgaa
     2461 ggggcgcggg gcgtcggcct gcgcgacctg cgacggcttc ttctaccgcg gcaagaaggt
     2521 cgcggtgatc ggcggcggca acaccgcggt cgaggaagcg atgtacctga ccaaccacag
     2581 ccatgacgtc acgctgatcc accgccgcga ttcgctgcgc gccgagaaga tcctgcagga
     2641 ccggctgttc gccaacccgg cgatcaaggt catctggaac cacgaggtcg ccgagttcat
     2701 cggcggcggc gaccccgagg ccttgatcgg cctcgacatc cgcgacacgc gctcgggcgc
     2761 ggtcagccgg atcgacgtcg agggcgcgtt cgtcgccatc ggccacaccc cggcgaccga
     2821 gctgttcgcc ggccagctcg acctcgacga ggacggctac atccgcgtcc aggacggctc
     2881 gacgcggacc agcgtcgagg gcgtgttcgc gtgcggcgac gtgatggaca agatctaccg
     2941 ccaggcggtg acggcggcgg gaaccggctg catggccgcg ctcgacgccg agaaatatct
     3001 cgccgcccgg gaattcgagg cgatggcggc ggaatgattc tttcccgtac cggggaggat
     3061 taaccgagcg gcacggccct tttctggccg gcctggcccg gggccggctc ggccgggtca
     3121 ttgccgagct tgatcgcccg gttggccgcg tcgacatgga cgacgagcgg cttcatccgc
     3181 ttcgcttcgg agtcgtcgac cacggcatag ctcatgatga tcaccaggtc gccggcgtgc
     3241 acgaggtggg cggcggcgcc gttgatggtg atcgtcccgc cgccgcgcgg ggcggggatc
     3301 acgtaggttt cgagccgggc gccgttggtg acgtcggcg
//
LOCUS       DASTZO010000161         2147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_665435,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000161 DASTZO010000000
VERSION     DASTZO010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665435"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFU20_04515"
     CDS             complement(<1..201)
                     /locus_tag="VFU20_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285759.1"
                     /translation="MKLRLVSLLVLSLLSACSRKGNVEEGGVFITRSACPLLGIPAAT
                     GDVTLFNPPGSRDASAIDVTATI"
     gene            292..1239
                     /gene="ispH"
                     /locus_tag="VFU20_04520"
                     /gene_synonym="lytB"
     CDS             292..1239
                     /gene="ispH"
                     /locus_tag="VFU20_04520"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEU5285760.1"
                     /translation="MAMALPPLHVLIAAPRGFCAGVDRAIQIVELALERFGAPVYVRH
                     EIVHNRFVVDRLRELGAVFVDELDEVPDDRPVVFSAHGVPKSVPAAAGERGLHYVDAT
                     CPLVSKVHRQAERLIADGRHILFIGHSGHPEVIGTFGQVPEGSMTLVETVEDAQNVAV
                     ADPATLAFLTQTTLSVDDTAAIVDALKARFPAIQAPRSEDICYATSNRQAAVKAIAPS
                     CDKMLVIGAPNSSNSLRLAEVAERIGVPARLIERASDIDWEWLGRPHTLGVTAGASAP
                     ELLVREVVDALRARFDVTEEEADHSPERLSFKLPRELVA"
     gene            1269..1688
                     /gene="rnhA"
                     /locus_tag="VFU20_04525"
     CDS             1269..1688
                     /gene="rnhA"
                     /locus_tag="VFU20_04525"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HEU5285761.1"
                     /translation="MFTDGACRGNPGPGGWAALIRAGARERELSGGEPLTTNNRMELK
                     AAIEGLNALKRPCRVKLTTDSNYVREGITRWIHGWQRNGWRTSDRKPVKNAELWQQLL
                     DAVARHRIEWHWVKGHSGHPENDRVDALACAEADARR"
BASE COUNT          369 a          731 c          731 g          316 t
ORIGIN      
        1 gatggtcgcg gtgacgtcga tcgccgaggc gtcgcggctg cccggcgggt tgaacaaagt
       61 gacgtcgccg gtcgcggcgg gaatcccgag cagcgggcag gcgcttcggg tgatgaacac
      121 gccgccttcc tcgacattgc cctttcgcga gcaggcggac agcagcgaca ggaccagcag
      181 cgagacgagg cggagcttca ctaggattcc ttcgaacggc gggacggcgg ttgggcccgc
      241 ttcataggaa gcgcctttca acgctgcaag cgatgcctta catggccggc gatggccatg
      301 gcccttcccc cgctgcatgt tctgatcgcc gctccgagag gcttttgcgc cggcgtcgac
      361 cgggcgatcc agatcgtcga gctggcgctc gagcggttcg gggcgccggt ctacgtgcgc
      421 cacgagatcg tccacaaccg cttcgtcgtc gaccggctgc gcgagctggg ggcggtgttc
      481 gtcgacgagc tggacgaagt ccccgacgac cggccggtgg tgttctccgc ccacggggtg
      541 cccaagtcgg tccccgccgc ggccggcgag cgcggcctcc attatgtcga cgccacctgc
      601 cccttggtct ccaaggtcca ccgccaggcc gagcggctga tcgccgacgg gcgccacata
      661 ttgttcatcg gccattccgg ccaccccgag gtgatcggca ccttcggcca ggtccccgag
      721 ggctcgatga ccctcgtcga gaccgtcgag gacgcgcaaa acgtcgccgt ggccgacccg
      781 gcgaccctcg ccttcctgac ccagacgacc ctgtcggtcg acgacacggc ggcgatcgtc
      841 gacgccttga aggcgcgctt ccccgcgatc caggcgccgc gcagcgagga catctgctac
      901 gcgacctcga accgccaggc ggcggtcaag gcgatcgcgc cgtcgtgcga caagatgctg
      961 gtgatcgggg ctcccaacag cagcaattcg ctgcgcctcg ccgaggtcgc cgagcggatc
     1021 ggggtcccgg cgcggctgat cgagcgcgcg agcgacatcg actgggagtg gctcggccgg
     1081 ccgcacacgc tcggggtgac cgccggcgct tcggcgcccg agctgctggt ccgcgaagtg
     1141 gtcgacgcgc tccgcgcccg cttcgacgtc accgaggagg aagccgacca ctcgcccgag
     1201 cggctgagct tcaagcttcc gcgggagctg gtcgcctgac catcccgggg gacctgcccg
     1261 aggtcgagat gttcaccgac ggggcgtgcc gcggcaatcc cgggcccggc ggctgggcgg
     1321 ccttgattcg tgcaggcgcg cgcgagcgcg agctgtcggg cggcgagccg ctgacgacca
     1381 acaaccggat ggagctcaag gcggcgatcg aggggctgaa cgccttgaag cggccatgcc
     1441 gggtgaagct caccaccgac agcaattacg tgcgcgaggg gatcacccgc tggatacacg
     1501 gctggcagcg caacggctgg cgcacctcgg acaggaaacc ggtcaagaac gcggagctgt
     1561 ggcagcagct gctcgacgcg gtcgcccggc accggatcga gtggcactgg gtcaagggcc
     1621 acagcggtca ccccgagaac gaccgcgtcg atgccctcgc ctgcgccgag gcggacgcac
     1681 ggcgctagcc tcgcgcttgc atagcgccaa tgtccgcttt cgacccattg cggacattca
     1741 gccaactgtt accgtagccg acatgacaga ccaaccagac caagccgctc gggacgagtg
     1801 gcagacacgg ctatacgatg cgagctatcg gttcggcata gcaatgcgag agttgaacga
     1861 cacgaaccct tggcccgata atcccgtgct tgcacaggca atcaacacgt tggcgacgga
     1921 actgtgggac aggtgcttta gcctgacgga aatcaagacg gcgttagaac aagctgccgc
     1981 cgacctgccg cgatatgcgg ctggccaaga ggttaggccc taacgtccgc tttccaccca
     2041 aagcggacat tggagccgcc gtcgacctgt cccgcatcgg cacgccctcg aagcggccgg
     2101 tttccacggc cgcccgggcg ggcaagatgg cggcatggac ttcgaca
//
LOCUS       DASTZO010000162         5843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_927742,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000162 DASTZO010000000
VERSION     DASTZO010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927742"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1152)
                     /locus_tag="VFU20_04530"
     CDS             complement(13..1152)
                     /locus_tag="VFU20_04530"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HEU5285762.1"
                     /translation="MRRPKPNQLPARPFFSSGPCAKPPGWSAARLDTGSLGRSHRSAP
                     GKARLSQAIERTHALLGLPADYRLGIVPASDTGAMELALWSLLGPRPVTMLAWESFGA
                     GWVTDVVKQLALEAEVRTAEYGQLPDLGGIDPASDVVFTWNGTTSGVRVPDAEWISAN
                     RSGLSICDATSAAFAQHIDWARIDVGTFSWQKALGGEGGHGMLVLSPRAVERLTSGPA
                     PRPLPKIFRLTKGGQLIDGIFKGETINTPSMLCVEDWLFALDWAEGVGGLSALIPRAD
                     SNAAALDRWVQGADWIEHLAVSPAIRSNTSVCLQFADRSDPQANAARQKAIVKLLEQE
                     GAAFDVGAYRDAPAGLRIWCGATVETADIEALGPWLDWAWEETGR"
     gene            1553..2599
                     /locus_tag="VFU20_04535"
     CDS             1553..2599
                     /locus_tag="VFU20_04535"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5285763.1"
                     /translation="MAQVLATNTAFFRDRDLFVHDGTKLRRFRLSAPVQAVFFLVLMA
                     LVAWSGYAAARMVTSGPQALSLSDATEARARKIEQRQALIEAIVTGQQIDEATLAAAA
                     AGGRATDNGPLARVEHAQLAQAALIARALDARYQTTAAELRKLGVAPSRVASVGGPFE
                     AVGKADPTFKQLFTNWKKLDTLQDGAIAVPSDKPVKTGAFTSGYGVRSDPFQGRAAMH
                     AGIDLAGPVGTAIYATADGVITDAGYNSGGYGNLVKIDHGRGIETRYGHLSKMSVYPG
                     QRVKRGELIGRMGSTGRSTGSHLHYEVRIDGRPVNPVPFMKSTDYLVAMQRKANTAPM
                     DTVALGGPSGGPRR"
     gene            2637..2969
                     /locus_tag="VFU20_04540"
     CDS             2637..2969
                     /locus_tag="VFU20_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEU5285764.1"
                     /translation="MSEADIILTENAARRVAAIARRQSKPAILRLAVDGGGCAGFTYK
                     FELAEAAEAGDTIAETGGVQLVVDPTSLDLVRGSAVDFVEDLGGAAFRVTNPNAASGC
                     GCGSSFSV"
     gene            2971..3747
                     /gene="xth"
                     /locus_tag="VFU20_04545"
     CDS             2971..3747
                     /gene="xth"
                     /locus_tag="VFU20_04545"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEU5285765.1"
                     /translation="MRLATFNLNGIRARLPRLLEWLERERPEIACLQELKCADESLPI
                     ADIEAAGYGAVWHGQKGFNGVAILARGDTPKLRRTGLPGDPDDSHSRYIEAEVGDFVV
                     ASLYLPNGNPVGTEKFDYKLRWMERLGSHAQELLADERPVVLAGDWNVVPEDRDVFSV
                     KATQHDALLQPETRAAYRRIVHQGWIDALRALHPDEERLYTFWDYTAGCWQRDAGFRI
                     DHLLCSPEAADRLRGAGVDKWARVQEKASDHAPTWVELAD"
     gene            complement(3744..4907)
                     /locus_tag="VFU20_04550"
     CDS             complement(3744..4907)
                     /locus_tag="VFU20_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEU5285766.1"
                     /translation="MPADVLVRPVACRADRRAFVDLPWAVYKDDPAWVPPLKSEVHAL
                     IDPGKNPWFGHARAQLWLATRGGRPVGRISAQVDELVEAHMGAGTGQWGMFEALDGEA
                     AAALIAIAEDWLSVAGMDRALGPISLSIWDEPGLEIHGFEQPPTAMMGHHRPEYRGWI
                     EAAGYAKARDLLTYEVGIAGWSDPLADRLVAAGEKNPRIRIRTVDKKRFDSEARIILN
                     LLNDAWSGNWGFVPLTEAEIAYAGKKLKPIIFEDLVRIAELATGPEPGAGFEAVAFMI
                     TIPDVNELIADLDGRLFPLGWARLLWRLRKPRTRRVRVPLMGVASKLHNSRLATQLAF
                     MLIEHTRRAAVAQFGATKGEFGWILEDNKGMVSIAQLPGARVNHIYRIFEKAL"
     gene            complement(4915..5667)
                     /locus_tag="VFU20_04555"
     CDS             complement(4915..5667)
                     /locus_tag="VFU20_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HEU5285767.1"
                     /translation="MAKGIWNRSHYLDRMTLKELWVAYFQYPAIIAYLALALVSAGVW
                     ALYPATPLQTLAAAGATVLAYPLVWYSLHRWVLHSTWMFKVPALARTWKRIHYDHHQD
                     PNHLEVLFGALHTTLPTIALVTLPIGYAIGGTGGAAAAFAAGLLTTCVYEFVHCIQHL
                     AYKPKWALVAEMKKRHMAHHFHDETGNFGITNYFWDRVMGTFYDRRERPEKSPNVFNL
                     GYTPEVAERYPWVARLSGGVATGHPRKRASRG"
BASE COUNT          952 a         2038 c         1984 g          869 t
ORIGIN      
        1 gcgtttggcc attcagcgac ccgtctcttc ccatgcccag tcgagccacg ggcccagcgc
       61 ctcgatgtcg gccgtttcga ccgtcgctcc gcaccagatg cgaaggcccg ccggggcgtc
      121 gcgataggcg ccgacgtcga acgccgcgcc ctcctgctcg agcaatttga cgatcgcctt
      181 ctggcgggcg gcgttcgcct gcgggtcgga gcggtcggcg aattgcaggc acaccgaggt
      241 gttcgagcgg atcgcgggcg atacggcgag gtgctcgatc cagtccgcgc cctgcaccca
      301 gcggtcgagc gccgcggcgt ttgagtcggc gcgcgggatc agcgcggaca ggccgccgac
      361 gccttccgcc cagtcgagcg cgaacagcca gtcctcgacg caaagcatcg agggcgtatt
      421 gatcgtctcg cccttgaaaa tgccgtcgat gagttgacca cctttggtca gccggaagat
      481 cttcggcagc ggccgcgggg ccggcccgct ggtcaatcgc tcgaccgcgc gcgggctgag
      541 caccagcatt ccgtggccgc cctcgccgcc gagcgccttt tgccagctga aggtgccgac
      601 atcgatcctg gcccagtcga tgtgctgggc gaaggcggcc gaggtcgcgt cgcagatcga
      661 gaggccgctg cggttcgccg agatccactc ggcgtcgggg acccgaaccc cgctggtggt
      721 gccgttccag gtgaagacca cgtcgctcgc cggatcgatc ccgccgaggt ccggaagctg
      781 cccgtattcg gcggtccgca cctcggcctc gagagccagt tgcttgacca cgtccgtgac
      841 ccaacccgcg ccgaagctct cccaggcgag catcgtcacc ggccgcgggc cgagcagcga
      901 ccacagggcc agctccatcg cgccggtgtc ggacgccggc acgatcccca gccggtagtc
      961 ggcgggcagg ccgagcagcg cgtgggtgcg ctcgatcgcc tggctcagcc gcgccttgcc
     1021 gggcgccgag cggtgcgagc ggccgagcga gcctgtatcc agccgcgccg ccgaccagcc
     1081 gggaggcttg gcgcagggcc ccgaggaaaa gaagggacgc gccggaagct ggttgggttt
     1141 gggccggcgc acggcggcgc cagcggcggg aaaattaacc aagacactct cctcacagag
     1201 agcacgcgcc gcgttgggac ggcgtggccc gccgccgctc tacggcacgg ggagcgaatg
     1261 acaagcgccc aggcgtgaaa gaatcacgct cccggccacc gcggaaaagc cgattttcgg
     1321 gtaagaatcc gctcattgtg gcccggtaac aacggttcat cgcagcgacc acgcggttca
     1381 tcccgctcac cggaacataa ggttgaaatc attaaccaag ccgggtcagg tttccctcgt
     1441 catcacgcgg tgggcaaatc cacgtggcgg cagcggggct ggggagctcc ttattcggtg
     1501 gacgaacgaa cgcgcatttg cgcggacgcg agggatttgg agcgaaaaaa ccatggcgca
     1561 ggttctggcc acgaacacag catttttccg cgatcgcgat ctcttcgtcc atgacggcac
     1621 caagctccgc cgcttccgcc tctccgcccc tgtccaggct gtcttcttcc tcgttctgat
     1681 ggcgctcgtc gcctggtccg gatatgcggc cgcgcgcatg gtcacgtcgg gcccgcaggc
     1741 gctgtcgctc agcgacgcga ccgaggcccg ggcccgcaag atcgagcagc gccaggcgct
     1801 gatcgaggcg atcgtcaccg gccagcagat cgacgaggcg acgctcgccg ccgcggccgc
     1861 cggcggacgc gcgaccgaca acgggccgct cgcccgcgtc gagcatgccc agctggcgca
     1921 ggccgcgctg atcgcccgcg cccttgacgc ccgctaccag actaccgccg cggaattgag
     1981 gaagctcggc gtcgccccgt cgcgcgtcgc ctccgtcggc ggaccgttcg aggcggtcgg
     2041 caaggccgac ccgacgttca agcaattgtt caccaactgg aagaagctcg acacgctcca
     2101 ggacggagcg atcgcggtcc cgtccgacaa gccggtcaag accggcgcct tcaccagcgg
     2161 ctatggggtc cgctccgacc cgttccaggg ccgcgccgcg atgcacgccg ggatcgacct
     2221 cgccggaccg gtcggaaccg cgatctacgc gaccgccgac ggggtcatca ccgacgccgg
     2281 ctacaacagc ggcggctacg gcaatctggt caagattgac cacggccgcg ggatcgagac
     2341 ccgctacggc catctgtcca agatgagcgt ctaccccggc cagcgcgtca agcgcggcga
     2401 gctgatcggc cgcatgggct cgaccggccg ctcgaccggc agccacctcc actacgaagt
     2461 gcggatcgac ggacgcccgg tgaacccggt cccgttcatg aagtcgaccg actatctggt
     2521 cgcgatgcag cgcaaggcga acacggcgcc gatggacacc gtcgccttgg gcggaccgag
     2581 cggcggcccg cgccgctagg gttgctgcgg acccaccccc accctatctc gcttgcgtga
     2641 gcgaggccga catcattctg accgagaacg ccgcgaggcg cgtcgcggcg atcgcccggc
     2701 gccagtcgaa gccggcgatc ctgcgactcg cggtcgacgg cggcggctgc gccggcttca
     2761 cctacaagtt cgagctcgcc gaggctgcgg aggccggcga cacgatcgcc gagaccggcg
     2821 gggtccagct ggtcgtcgac ccgaccagcc tcgacctggt gcgcggctcg gcggtcgact
     2881 tcgtcgagga tttgggcggc gccgcgttca gggtgaccaa cccgaacgcc gcctcgggct
     2941 gcggctgcgg ctcgagcttc tccgtgtgaa gtgaggctcg cgaccttcaa cttgaacggc
     3001 atccgagcgc gcctgccgcg gctgctcgag tggctcgagc gcgaaaggcc cgaaatcgcc
     3061 tgcctccagg aactgaagtg cgccgacgaa tcgctgccca tcgccgacat cgaggccgcc
     3121 ggctacgggg ccgtgtggca cgggcagaag gggttcaacg gggtcgcgat cctcgcccgc
     3181 ggcgacactc cgaagctgcg ccgaaccggc cttcccggcg acccggacga cagccacagc
     3241 cgctacatcg aggccgaggt cggcgacttc gtcgtcgcct cgctctacct gcccaacggc
     3301 aatccggtcg ggaccgagaa gttcgactat aagctccgct ggatggagcg gctcggaagc
     3361 cacgcacagg agctgctcgc cgacgagcgg ccggtggtcc tcgccggcga ctggaacgtg
     3421 gttcccgagg accgcgacgt cttctcggtc aaggcgacgc agcacgacgc gctgctgcag
     3481 cccgagactc gcgccgccta ccgccgaatc gtccaccagg gctggatcga cgcgctccgg
     3541 gcgctgcacc cggacgagga gcggctctac accttctggg actataccgc cggctgctgg
     3601 cagcgcgacg cgggcttccg gatcgaccat ctgctgtgct cgcccgaagc cgccgaccgg
     3661 ctgcgcggcg cgggcgtcga caaatgggcg cgggtgcagg agaaagcgag cgaccacgcg
     3721 ccgacctggg tcgagctggc cgactagagc gctttttcga agatccggta gatgtggttg
     3781 accctcgcgc cgggcagctg ggcgatggac accatcccct tattgtcctc gaggatccag
     3841 ccgaactcgc ccttcgtggc gccgaattgg gcgaccgcgg cgcggcgcgt gtgctcgatc
     3901 agcatgaagg cgagctgggt cgcgaggcgg ctgttgtgga gcttcgaggc gacccccatc
     3961 agcggcaccc gcacccgccg cgtccgcggc ttcctgagcc gccacagcag ccttgcccag
     4021 cccagcggga acagccggcc atcgagatcg gcgatcagct cgttgacgtc gggaatggtg
     4081 atcatgaacg ccacggcctc gaagcctgcg ccgggctccg gcccggtagc cagctcggcg
     4141 atgcggacca gatcctcgaa gatgatcggc ttcagcttct tgcccgcata ggcgatctcc
     4201 gcctcggtca gcgggacgaa gccccaattg cccgaccagg cgtcgttgag caggttgagg
     4261 atgatccgcg cctcgctgtc gaagcgtttc ttgtcgacgg tgcggatccg gatgcgcggg
     4321 ttcttctcgc ccgccgcgac cagccggtcg gcgagcgggt cggaccagcc ggcgatcccg
     4381 acctcgtagg tcaagaggtc cctggccttg gcgtagcccg ccgcctcgat ccagccgcga
     4441 tactcggggc ggtggtggcc catcatcgcc gtcggcggct gctcgaagcc gtggatctcg
     4501 agccccggct cgtcccagat cgacaggctg atcggcccga gcgcccggtc catgcccgcc
     4561 acgctcagcc agtcctcggc gatcgcgatc agcgccgccg cggcctcccc gtcgagcgcc
     4621 tcgaacatgc cccactggcc ggtgccggcg cccatgtggg cctcgaccag ctcgtcgacc
     4681 tgggcgctga tccggccgac gggccggccg ccgcgtgtcg ccagccacag ctgcgcgcgg
     4741 gcatggccga accacgggtt cttgccgggg tcgatcaaag cgtggacttc gcttttcagc
     4801 ggcggcaccc aggccggatc gtccttgtag accgcccagg gaaggtcgac gaacgccctc
     4861 cggtcggccc ggcaagcgac cggccggacg agcacgtcag ccggcatatt gggcttagcc
     4921 gcgcgaagcg cgcttgcgcg ggtggccggt cgcgactccg cccgacagcc gggcgaccca
     4981 cggatagcgc tccgccacct cgggcgtgta gccgaggttg aacacgttgg ggctcttctc
     5041 gggccgctcg cggcggtcgt agaaggtgcc catcacccgg tcccagaaat agttggtgat
     5101 gccgaaattg ccggtttcgt cgtggaaatg gtgggccatg tggcgcttct tcatctcggc
     5161 gaccagcgcc cacttgggct tgtaggcgag gtgctggatg cagtggacga actcgtagac
     5221 gcaggtcgtc agaagccccg ccgcgaacgc cgccgcggcg ccgccggtgc cgccgatcgc
     5281 atagccgatc ggcaaagtga cgagggcgat ggtcggcaag gtcgtgtgca gcgccccgaa
     5341 cagcacctcg aggtggttgg ggtcctggtg atggtcgtaa tggatccgct tccaggtccg
     5401 ggccagcgcc ggcaccttga acatccaggt cgagtgcagc acccagcggt gcagcgaata
     5461 ccacaccaaa ggataagcga gcaccgtcgc cccggccgcg gccagggtct gcagcggggt
     5521 cgccggatag agcgcccaca cgccggccga caccagcgcc agcgcgaggt aggcgatgat
     5581 cgccggatac tggaaataag cgacccacag ctccttgagc gtcatccggt caaggtagtg
     5641 cgatcggttc caaataccct tagccatggt ctctgcagtc acgaaatgtc cttgagcaat
     5701 gccgctgccc gggcggcatt accgatgtgg gactctaata aggcggtttt gcgacaggga
     5761 aagggtcgga aggcggctgg ggcgcctcct ccggccgcgc ggaaagtcgc aaaagtcgca
     5821 ctccaacgcg cgcgtgatcg cgt
//
LOCUS       DASTZO010000163         3217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_875596,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000163 DASTZO010000000
VERSION     DASTZO010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875596"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1024
                     /locus_tag="VFU20_04560"
     CDS             <1..1024
                     /locus_tag="VFU20_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5285768.1"
                     /translation="SRLTGNLGVQAVRTEQESTGAVITGNIFDRATLGDEYWDVLPNL
                     NLSLRTPADLVVRFALARQMQRPRLDQMRVAFGYGVDRTTDPTNPTIRGGGGNPELRP
                     YRANAVDLTVEKYFGNRGYLAAQLFYKQLKNFIYEQEVPYDYTGLPLDDVIAPGGTIP
                     SGIPGFITQPVNAKGGKIYGFEIAGTLPFGEFIPVLDGFGVTGGYGYTKSRVRPSPSE
                     SYGDIPGYSRHVANGTLFFEKWGFGARVSARYRSTFVGEFSGFGGNRVRRRAQGETLV
                     DAQIGYDLGAGIGAGSMFKGLSVFAQASNLTDEPFATINPGAPFEVIDYQTYGRRYQA
                     GITYKF"
     gene            1082..2596
                     /locus_tag="VFU20_04565"
     CDS             1082..2596
                     /locus_tag="VFU20_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan halogenase family protein"
                     /protein_id="HEU5285769.1"
                     /translation="MKIVIVGGGSAGWMAAAALGRIIGEQWPIELVESEQIRTVGVGE
                     ATIPQILLFNGVVGLDEAEFIRATKGSFKLGIEFVGWGAPDERYIHAFGLVGRDLGLI
                     PFHHYWLQARARGDRSGLSEYSLSAMAAASNVFMRKDPLPRTPIGAATYAYHFDASLY
                     GAHLRTHAEKWGVKRHEGRITSVGRDGGSGHVTHVELEDGRRIEGDLFIDCSGFRALL
                     IGETMGSAYVDWSQWLPCDRALAVPCESVEPLTPVTRATAREAGWQWRIPLQHRIGNG
                     YVYCSDFVSDEAAAKTLMANLDGKALGEPRPLRFTAGRRQDFWRGNVVALGLASGFLE
                     PLESTSIHLVQSGIKHLVDLLPRDGIDPADVAAYNSKLAFEYEKIRDFLILHYWANGR
                     SEPFWKRCREMEIPDTLVEKVETFRSHGRIFRFNEELFTEVAWLQVMVGQNILPRSYH
                     PLADAPGDDNVDQYLASIRELIAAKVARMPGHRDYLERLAGTQDAQAIATEVPA"
     gene            2632..>3217
                     /locus_tag="VFU20_04570"
     CDS             2632..>3217
                     /locus_tag="VFU20_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU5285770.1"
                     /translation="MLAGAAAPAAAQPDYRARLPQDEVIYFLLPDRFENGDPGNDLGG
                     FPGGRLQTGFDPTHKGFFHGGDLKGLTRRLGYIQRLGATAIWLAPIFKNKPVQGPPGN
                     ESAGYHGYWVTDFTQVDPHFGTNTDFKAFVDAAHARGMKVYMDIIANHTADVLYFAEC
                     EGTGQCPYRSVGDYPYQARGGIQGRRINRGFAGHE"
BASE COUNT          547 a         1089 c         1066 g          515 t
ORIGIN      
        1 gtcgcggctg accggaaacc tcggcgtcca ggccgtgcgg accgaacagg agtcgaccgg
       61 cgcggtcatc accggcaaca ttttcgaccg ggcgacgctg ggcgacgaat attgggacgt
      121 gcttcccaac ctcaacctgt cgctgcgcac gcccgccgat ctggtcgtcc gcttcgctct
      181 ggcgcggcag atgcagcggc cgcgcctcga ccagatgcgg gtggcgttcg gctatggcgt
      241 cgaccggacg accgacccga ccaacccgac catccgcggc ggcggcggca atcccgagct
      301 tcgcccgtac cgggccaatg ccgtcgacct cacggtcgag aaatatttcg gcaaccgcgg
      361 ctatctggcg gcgcagctgt tctacaagca gctgaagaac ttcatctacg agcaggaagt
      421 gccgtacgac tataccggcc tgccgctcga cgacgtgatc gcgcccggcg gcaccatccc
      481 atcaggcatt cccggcttca ttacccagcc ggtcaacgcc aagggcggga agatctacgg
      541 gttcgagatt gccggcacgc tgccgttcgg ggagttcatc ccggttctcg acggcttcgg
      601 agtgaccggc ggctatgggt acaccaagag ccgggtccgg ccgtcgccca gcgagagcta
      661 cggcgacatt ccgggctatt cgcgccatgt cgccaacggc accctgttct tcgagaaatg
      721 gggcttcggg gcacgggtca gcgcgcgcta ccgctcgacc ttcgtcggcg aattctccgg
      781 cttcggcggc aaccgggtac gccgccgggc gcaaggcgag actctggtcg atgcgcagat
      841 cggctacgat ctcggggccg gaattggcgc cggaagcatg ttcaaggggc tctcggtctt
      901 cgcgcaggca tcgaacctta ccgacgagcc gttcgcaacg atcaaccccg gagctccttt
      961 cgaggtgatc gactatcaga cctatgggcg ccgctatcag gccggcatca cctataagtt
     1021 ctgacacgat ggcggcggtt cggggctgcc cggccgccgt cgcgaatgaa gaagtaggcc
     1081 gatgaaaatc gtcatcgtcg ggggcggctc ggccggatgg atggccgccg ccgcgctcgg
     1141 ccggatcatc ggcgagcaat ggccgatcga gctcgtcgaa tccgagcaga tcagaaccgt
     1201 cggagtcggc gaggcgacga tcccgcagat cctgctgttc aacggcgtcg tcgggctcga
     1261 cgaggccgag ttcatccgcg ccaccaaggg cagcttcaag ctcggcatcg aattcgtcgg
     1321 ctggggagcg cccgacgagc gctacatcca cgccttcggg ctggtcgggc gggacctggg
     1381 gctgatcccg ttccaccatt attggctcca ggcgcgcgcg cgcggcgacc ggtccggcct
     1441 ttccgaatac agcctgtcgg cgatggccgc ggcgagcaac gtcttcatgc gcaaggaccc
     1501 gctgccgcgc accccgatcg gagccgcaac ctacgcctac catttcgacg ccagcctcta
     1561 tggggcgcac ctgcgcaccc atgccgagaa atggggcgtc aagcggcatg aggggcggat
     1621 cacgagcgtc ggcagggacg gcggcagcgg ccatgtcact cacgtcgagc tcgaggacgg
     1681 ccgccggatc gaaggcgatt tgttcatcga ttgctcgggc ttccgggcgc ttctgatcgg
     1741 cgagacgatg ggcagcgctt acgtcgactg gtcgcaatgg ctgccgtgcg accgggccct
     1801 ggcggtgccg tgcgaatcgg tcgagccgct gacgccggtc acgcgcgcga ccgcccgcga
     1861 agccggctgg cagtggcgga tcccgctcca gcaccggatc ggcaacggct acgtctattg
     1921 ctcggatttc gtcagcgacg aggcggcggc caagacgctg atggcgaatc tcgacggcaa
     1981 ggcgctcggc gagccgcgcc cgctcaggtt caccgccggg cggcggcagg atttctggcg
     2041 cggcaacgtc gtcgcgctgg gcctcgcgag cggcttcctc gagccgctgg agtcgaccag
     2101 catccatctc gtccagtcgg gcatcaagca cctggtcgac ctgcttccgc gtgacggcat
     2161 cgacccggcc gacgtcgccg cctacaattc gaagctggcg ttcgaatatg agaagatccg
     2221 cgacttcctg atcctgcatt attgggcgaa cgggcgcagc gagccgttct ggaagcgctg
     2281 ccgcgagatg gagatcccgg acacgctggt cgagaaggtc gagacgttcc gctcgcacgg
     2341 ccgcatcttc cgcttcaacg aggaactgtt caccgaggtc gcctggctgc aggtgatggt
     2401 cgggcagaac atcctgccgc gcagctacca cccgctcgcc gacgcgcccg gcgacgacaa
     2461 tgtcgaccaa tatctcgcgt cgatccgcga gctgatcgcg gccaaggtgg cgaggatgcc
     2521 cggccaccgc gactatctcg aacggttggc cgggacgcag gacgcgcagg cgattgcaac
     2581 ggaggtgccg gcatgactgg aagattgccg ttatggctgt tcggcgcggc catgctcgct
     2641 ggcgccgccg cgcccgccgc tgcccagccc gattaccgcg cccgcctgcc gcaggacgag
     2701 gtgatctatt tcctgcttcc cgaccggttc gagaacggcg acccgggcaa cgacctcggc
     2761 gggtttccgg gggggcggct gcagaccggc ttcgatccca cccacaaggg cttcttccac
     2821 ggcggcgacc tcaagggcct gacccggcgg ctcggctata tccagcggct cggggcgacg
     2881 gcgatctggc tggcgccgat cttcaagaac aagccggtgc agggcccgcc cggcaacgaa
     2941 agcgccggct accacggcta ttgggtgacc gacttcaccc aggtcgaccc gcatttcggc
     3001 accaacaccg acttcaaggc cttcgtcgac gcggcgcacg cgcgcggcat gaaggtctac
     3061 atggacatca tcgccaacca caccgccgac gtgctctatt tcgccgagtg cgagggcacc
     3121 ggccaatgcc cgtaccggag cgtcggcgat tatccgtacc aggcgcgcgg cggcatccag
     3181 ggcaggcgca tcaaccgcgg ctttgccggc cacgagg
//
LOCUS       DASTZO010000164         3798 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_510183,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000164 DASTZO010000000
VERSION     DASTZO010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510183"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VFU20_04575"
     CDS             <1..632
                     /locus_tag="VFU20_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEU5285771.1"
                     /translation="TKLDDGTSQAILPTPAIGGVGLLEDWEKSATIGFKAEGETILVI
                     GGTVGHLGQSLWLSEIHARRDGPPPPADLIEERANGEFVRALVSAGLVTAVHDVSDGG
                     LAVAVAEMALAGGLGARIAAAVDGCPTAAMAFGEDQGRYVVTTRDPDAILARAGRADR
                     FAAPIGVTGGESIIGRLDQGPEWRVALADLCAAHEGFFPNLMGAEGVLA"
     gene            683..877
                     /locus_tag="VFU20_04580"
     CDS             683..877
                     /locus_tag="VFU20_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5285772.1"
                     /translation="MIVCVCNAITEDEVRQAARAGATSPDKAYAHLGCEPHCGCCLDY
                     AQELIEQERGKRPRLRVVAA"
     gene            1004..1483
                     /gene="bfr"
                     /locus_tag="VFU20_04585"
     CDS             1004..1483
                     /gene="bfr"
                     /locus_tag="VFU20_04585"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HEU5285773.1"
                     /translation="MKGDPKVIELLNEALKNELTATNQYWLHYRLLDNAGVKRLAEFE
                     RSESIDEMKHADRLAERILFLDGLPNFQMLGRLRIGENVEEILRADLELELEALTQLR
                     GAVGHCEKVKDYVSRDLFSEILENEEEHVDTLEQQFELIAAMGLPNYIQLQSEPAKE"
     gene            1517..2197
                     /locus_tag="VFU20_04590"
     CDS             1517..2197
                     /locus_tag="VFU20_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEU5285774.1"
                     /translation="MTDAAYKPIAWPGPPALPPEEMIARARAFHERIASRRTCRFFSS
                     EPVPRAVIEQAILAAGTAPSGANHQPWHFAVVSSPDAKRAIRDAAEKEEQAFYAGKAG
                     AEWLEALARLGTDADKPYLETAPWSIVVFGQRRGGIEPGDDRQNYYVTESVGIACGFL
                     LLALHEAGLATLTHTPNPMRFLNRLCGRPPNEKPVMVIVTGRPAPDATVPAHALRKKP
                     LDRIASWI"
     gene            2217..3488
                     /locus_tag="VFU20_04595"
     CDS             2217..3488
                     /locus_tag="VFU20_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HEU5285775.1"
                     /translation="MTATTIAQRILTLDIVRGVAVMGILAMNIVAFAMPPQAYINPLA
                     YGTESAADLASWAFSFVFVDGKMRGLFSFLFGASMLLVIQLAEKCGDSARRVTFARQW
                     WLLLFGMIHYYFIWFGDILIGYALIGMAAWLFRRLEPRALVRWGIVLVTVQLMVMSSM
                     AAYAHSLSAAVAAGNAGPEIVEQWAEFSRDIAIPSAALLREEMALYLGPWSGIAEYQL
                     TEKAVMPLFFTFLFGWETLAYMLFGMAALKSGFLTGAWDNARYRRVALTALAAAIPVY
                     VVLAGLLFADDFSVPGIFTYSFAATVPFRPMMVVAYAALIILATRRGGRLVDRIAATG
                     RAAFSNYLGTSILMTGLFYGWGLGWFGSVSRVELWPVVIGMWLAMLAWSKPWLDRFQY
                     GPLEWLWRSLSRWQVQPFRKRLATAPAAAGA"
     gene            complement(3485..>3798)
                     /locus_tag="VFU20_04600"
     CDS             complement(3485..>3798)
                     /locus_tag="VFU20_04600"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HEU5285776.1"
                     /translation="QIELAMHTGNTAGLVIPAHTIKGEARQFGAEPLAAVAELIESTA
                     RLCIETQRFPDELVKDVVELKRLFQTTVEQFDKATNPLLTRAPQGAFGRKATNQNFGR
                     L"
BASE COUNT          583 a         1292 c         1260 g          663 t
ORIGIN      
        1 agaccaagct cgacgacggc accagccagg ccatcctccc gacccccgcg atcggcggcg
       61 tcggcctgct ggaggactgg gagaaaagcg cgacgatcgg gttcaaggcc gagggcgaga
      121 cgatcctcgt gatcggcggc accgtgggcc acctcggcca gtcgctctgg ttaagcgaaa
      181 tccacgcccg ccgcgacggt ccgccgccgc ccgccgattt gatcgaggag cgggccaacg
      241 gcgagttcgt gcgcgcgctc gtttccgctg ggctggtgac cgccgtccac gacgtgtcgg
      301 acggcgggct ggcggtcgcg gtcgccgaaa tggcgctggc gggcgggctc ggcgcccgga
      361 tcgcggcggc ggtcgacggc tgccccacgg cggccatggc gttcggcgaa gaccagggcc
      421 ggtatgtcgt aacgacccgc gacccggacg caatcctcgc ccgggccggg cgcgccgacc
      481 gctttgcggc ccccattgga gtgaccggcg gcgagtcgat catcgggcgg ctcgaccagg
      541 gccccgagtg gcgcgtggcc ctcgccgacc tctgcgccgc gcacgagggc ttctttccca
      601 atctgatggg cgccgagggc gtcctcgcat aagcagctcc tttctattgc gaatgggtcg
      661 cattagcagt atatcagcgc ccatgatcgt ctgcgtttgc aatgccatca ccgaggacga
      721 ggtccggcag gcggcgcgcg ccggcgcgac ctcgccggac aaggcttacg cgcacctcgg
      781 ctgcgagccg cattgcggct gctgcctcga ttatgcgcag gagctgatcg agcaggagcg
      841 cggcaagcgc ccgaggcttc gggtggtcgc cgcctgaacc cgcgattgcg actgactatc
      901 agtcgcagat tttccgcaga tttccgccga ttttaaagct tcccatgcgg cgccggcaag
      961 agtaagcccg cgcccacgag tgccgtacaa ggggaggcgt gcaatgaagg gcgaccccaa
     1021 ggtcatcgag ctcttgaacg aagcgctcaa gaacgagctg accgcgacca accagtattg
     1081 gctgcactac cggctgctcg acaatgccgg ggtcaagcgc ctcgccgagt tcgagcgctc
     1141 cgaatcgatc gacgagatga agcacgccga ccggctcgcc gagcgaatcc tgttcctgga
     1201 cggcctgccc aacttccaga tgctcggccg gctgcggatc ggcgagaatg tcgaggagat
     1261 cctgcgcgcc gacctcgagc tcgaactgga ggcgctgacc cagttgcgcg gcgccgtcgg
     1321 gcattgcgaa aaggtcaagg actatgtcag ccgcgacctg ttctccgaga tcctcgagaa
     1381 cgaggaggag catgtcgaca cgctcgagca gcagttcgag ctgatcgcgg cgatgggcct
     1441 tcccaattac atccagctgc agtcggagcc ggcgaaggaa tagcgccacg cccgggttgc
     1501 gtgcacaagc catgccatga ccgacgccgc ttacaagccg attgcgtggc cggggccccc
     1561 cgcactgccg cccgaggaaa tgatcgcccg cgcacgcgcc ttccatgaac ggatcgcgag
     1621 ccgccgcacc tgccgcttct tttcctccga gccggttccc cgcgcggtca tcgagcaggc
     1681 gatcctcgcc gccgggacgg cgccatcggg agccaatcac cagccttggc acttcgccgt
     1741 ggtttcatca cccgacgcca aacgcgcgat ccgcgatgcg gccgaaaagg aagagcaggc
     1801 cttctatgcc ggcaaggccg gtgccgagtg gctcgaagcg ctggcgcggc tcggcaccga
     1861 tgccgacaag ccgtatctgg agaccgcgcc gtggtcgatc gtcgttttcg gccagcgccg
     1921 tggcggtatc gaacccggcg acgaccgtca gaactattat gtgaccgaga gcgtcggcat
     1981 cgcctgcggc ttcctgttgc tggcgctgca cgaggccggc ctcgcgaccc tcacgcacac
     2041 tcccaacccg atgcgcttcc tcaatcgcct gtgcggccgg ccgccgaacg aaaagccggt
     2101 gatggtgatc gtcaccggcc gccccgcgcc cgacgccacg gtgcccgctc atgcgctcag
     2161 gaaaaagccg ctcgaccgga ttgcaagctg gatttaactg ggctccgggg gctgaattga
     2221 ccgcaaccac catcgcccag cgcatcctga ccctcgacat tgtccgcggg gtcgcggtga
     2281 tgggcatatt ggcgatgaac atcgtcgcct tcgccatgcc gccgcaggcc tatatcaacc
     2341 cgctcgccta cggcaccgag agcgccgccg atctcgcctc gtgggcgttc agcttcgtct
     2401 tcgtcgacgg caagatgcgc ggactgttct cgttcctgtt cggggcctcg atgctgctgg
     2461 tcatccagct cgccgagaaa tgcggcgatt cggccaggcg cgtgaccttc gcccgccagt
     2521 ggtggctgct gctgttcggg atgatccatt attacttcat ctggttcggc gacattctga
     2581 tcggttacgc gctgatcgga atggccgcct ggctgttccg ccggctggag ccgcgggcgc
     2641 tggtccgctg gggcatcgtc ctggtgaccg tccagctgat ggtgatgagc tccatggccg
     2701 cctacgccca ttcgctgagc gccgcggtcg ccgccggcaa cgccgggccc gaaattgtcg
     2761 agcaatgggc cgaattcagc cgcgacatcg ccatccccag cgcggctctg ctgcgcgagg
     2821 aaatggcgct ctacctcggc ccgtggtcgg ggatcgccga gtaccagctc accgaaaagg
     2881 ccgtgatgcc gctgttcttc accttcctgt tcggctggga gacgcttgcc tacatgctgt
     2941 tcgggatggc ggcgctgaag agcggcttcc tgaccggcgc gtgggacaat gcccgctacc
     3001 gccgtgtcgc gctgaccgcg ctcgcggccg cgatccccgt ctatgtcgtg cttgccgggc
     3061 tgctgtttgc cgacgatttc tcggttccag ggattttcac gtattcgttc gcggcgacgg
     3121 tgccgttccg gccgatgatg gtcgtcgcct acgcggcgct gatcatcctc gccacgcgcc
     3181 gcggcggccg gctggtcgac cgcatcgccg ccaccggccg ggccgcgttc agcaattatc
     3241 tcggcacgtc gatcctgatg accggcctgt tctacggctg ggggctcggc tggttcggca
     3301 gcgtcagccg ggtcgagctg tggccggtgg tgatcggcat gtggctggcg atgctcgcct
     3361 ggtcgaagcc gtggctcgac cgcttccaat acggcccgct cgaatggctg tggcggagcc
     3421 tctcccgctg gcaggtgcag ccgttccgca agcggcttgc gacagcgccg gctgccgccg
     3481 gggcctaaag ccgtccgaaa ttttggttgg tggccttgcg cccgaacgcg ccctgcggcg
     3541 cccgggtcag cagcgggttg gtcgccttgt cgaactgctc gacggtcgtc tggaacagcc
     3601 gcttcagttc gaccacgtcc ttgaccagct cgtcggggaa gcgctgggtc tcgatgcaca
     3661 gccgggcggt gctctcgatc aattcggcga ccgcggccag tggctcggcc ccgaactggc
     3721 gcgcctcgcc cttgatggtg tgcgccggga tcaccagccc ggcggtgttg ccggtgtgca
     3781 tcgccagctc gatctggg
//
LOCUS       DASTZO010000165         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_976316,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000165 DASTZO010000000
VERSION     DASTZO010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976316"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1436)
                     /locus_tag="VFU20_04605"
     CDS             complement(69..1436)
                     /locus_tag="VFU20_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU5285777.1"
                     /translation="MTTLRNLGAARRSTPGFGKAARIAALLPLAAATLSIAHSAPAAA
                     QRDPAPRAVPGIGNDIAAFYRSRGGAPLWFSPTAGPAPHQLLQLLSTAAADNMNPNRY
                     RVRQLAKAVQAAATRRNPAAVQRAEVMLSEAFVTYARDLRRDPNVGIIYVDPELRPVP
                     PSPRSLLDQAAASGSISKYIERMGWMSPVYAQLRRALAARMYRSELERQLLVVNLERA
                     RALPSGNHRYAVVNPAAQRLYMYEDGQVVDMMRVIAGKPAPPIAQTPMMSALIRFVVL
                     NPYWNAPPDITAQKLAPNVLKQGRSYLKAKGYQVMSDWTDGARVLDPGTVDWKAVVAG
                     RVPIRLRQLPGPANSMGRMKFMFPNEQGIWLHDTPQKELLDEAARLQSNGCVRLQDAP
                     RFARWLFGRPLKAQGTKPEQSVPLPVPVPVYITYLTAVPSGSSIVYFDDVYGLDRRDR
                     RLASR"
     gene            complement(1480..2772)
                     /locus_tag="VFU20_04610"
     CDS             complement(1480..2772)
                     /locus_tag="VFU20_04610"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU5285778.1"
                     /translation="MLAAAPLPSFAATAATAAGTAQPAGFSDSRGGVAEFYRARDGRP
                     LWLAPESGAAAQLMLGLLNSASIDGLDPDRFPVRRIADALRAARNGDRRAIGRAESML
                     SQAFVAYARELRQAPNIGIVYVDRELVPAAPSARYLLESAAAASSLENFVDTMGWMNP
                     VYGQLRRAVMSRSYANDLQRRTLLLNLERARALPAGNLRHVVVNAAAQRLYMYEDGHL
                     VDSMRVVVGKPKHATPMMNAFIRAAALNPYWNVPPDLAAERIAPNVVEQGVRYLNAHG
                     YQLLSDWSDNPAIVDPATIDWKAVAAGRIEVRIRQLPGPQNALGKMKFVFPNAQGIFL
                     HDTPERQLLSEASRLYSGGCIRLEDAPRFGRWLFGRDLRKQGDAPEQIVPIPTLVPLY
                     VTYLTAVPSGASIAYFDDVYGRDAARLAAVGAGHYASR"
     gene            complement(2866..3354)
                     /locus_tag="VFU20_04615"
     CDS             complement(2866..3354)
                     /locus_tag="VFU20_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HEU5285779.1"
                     /translation="MAGDVAEGAAYGAAGGAAIGAVVPGVSMIEGAAVGAAIGGLANA
                     VWADRNNDGYVDGYVQNGQYYAGTPQGYDPTLRRVATGALGGAALGAAAGAVIPGLSV
                     LEGAVIGAAVGGVAGAIWADRDNDGRVDGYVYNGQYYAGAPTTTAPAPTYSAPMRSGE
                     RG"
BASE COUNT          546 a         1140 c         1232 g          537 t
ORIGIN      
        1 gatcgcccag gacatgtcct acaccccgac ctggcggccc aattactgaa ggatcgcggg
       61 cctttcgact agcggctggc caggcggcgg tcgcgccggt cgagcccgta gacgtcgtcg
      121 aaatagacga tcgaagagcc gctcgggacg gccgtcagat aggtgatgta caccggcacc
      181 gggaccggca gcggcacgga ctgctcgggc ttggtgccct gcgccttgag cggccggccg
      241 aacagccagc gcgcgaaccg cggcgcgtcc tgcagcctca cgcagccgtt gctctgcaag
      301 cgggcggcct cgtcgagcaa ctccttttgg ggcgtgtcgt ggagccagat cccctgctcg
      361 ttggggaaca tgaacttcat ccggcccatc gaattggcgg ggccgggcag ctggcgcagg
      421 cgaatcggga cgcgcccggc gacgacggcc ttccagtcga cggtgcccgg atcgagcacc
      481 ctggcgccgt ccgtccagtc ggacatcacc tgatagccct tggccttcag atagctgcgt
      541 ccctgcttga ggacgttggg cgcgagcttc tgggcggtaa tgtccggcgg cgcgttccag
      601 taggggttga ggaccacgaa gcggatcagg gcgctcatca tcggggtctg agcgatcggc
      661 ggcgcgggct tgccggcgat gacccgcatc atgtccacca cctggccgtc ctcgtacatg
      721 tacagccgct gggcggccgg attgaccacc gcatagcgat ggttgcccga gggcagcgcg
      781 cgcgcccgct cgagattgac caccagcagc tggcgctcga gctcgctgcg gtacatgcgc
      841 gcggcgagcg cccggcgaag ctgggcatag accgggctca tccagcccat ccgctcgata
      901 tatttcgaaa tcgagccgga ggccgccgcc tggtcgagca gggaccgcgg cgacggcggg
      961 accggccgaa gctcggggtc gacgtagatg atgccgacgt tggggtcgcg ccttaggtcg
     1021 cgcgcatagg tcacgaacgc ctcgctcagc atcacttcgg cccgctgcac cgcggccgga
     1081 ttgcggcggg tggcggcggc ctggaccgcc ttggcgagct gcctcacccg gtagcggttg
     1141 gggttcatgt tgtcggccgc ggccgtgctt agcagctgca gcaactggtg gggcgcgggg
     1201 cccgcggtcg gcgagaacca caatggagcg ccgccgcgcg atcgatagaa tgcggcgatg
     1261 tcgttgccga tccccgggac ggcgcgcggc gccggatcgc gctgggccgc ggccggagcg
     1321 gaatgggcga tcgacagggt cgccgcggcc agcggcagca gagccgcgat tcgggcggct
     1381 ttcccgaaac cgggtgtcga tcgcctggcg gcgcctaagt tacgcaaagt ggtcactttt
     1441 tctccgatgc tcgtcatatg gagccggtgc ccgcgatggt caacggctcg cgtaatgccc
     1501 cgccccgacg gctgcaagcc gcgccgcgtc gcgcccgtag acgtcatcga aataagcgat
     1561 cgacgcgccg ctcggaacgg cggtcaggta ggtgacgtag agcgggacca gggtcgggat
     1621 gggaacgatc tgctcgggtg cgtcgccctg tttcctgagg tcgcggccga acagccagcg
     1681 gccgaagcgg ggcgcgtcct cgaggcggat gcagccgccg ctgtacaggc gcgacgcctc
     1741 gctcagaagc tgccgttccg gagtgtcgtg gaggaaaatg ccctgggcgt tggggaacac
     1801 gaacttcatc ttccccaggg cgttctgggg tcccggaagc tggcggatcc tgacctcgat
     1861 ccggccggcc gcgaccgctt tccagtcgat ggtggcggga tcgacgatgg ccggattgtc
     1921 gctccagtcc gacagcagct ggtatccgtg ggcgttgaga tatcggacgc cctgctcgac
     1981 gacattgggc gcgatccgct cggcggcgag gtcgggcggc acgttccaat aggggttgag
     2041 ggcggcggcg cggatgaacg cgttcatcat cggcgtcgcg tgcttgggct tgccgaccac
     2101 gacccgcatc gagtcgacca gatggccatc ctcgtacatg tacagccgct gggccgccgc
     2161 gttgaccacg acgtggcgca gattgcccgc cggaagcgcc cgcgcgcgct ccaggttgag
     2221 aagcagggtc cgcctctgca ggtcgttggc ataggagcgg ctcatcacgg ctcggcgaag
     2281 ctgcccgtaa accgggttca tccagcccat cgtgtcgacg aaattctcga gcgaggacgc
     2341 cgccgcggcg ctttcgagaa ggtagcgcgc ggaaggagca gccggaacca gctctcggtc
     2401 gacatagacg atgccgatgt tcggggcctg ccgaagctcg cgcgcgtagg cgacgaacgc
     2461 ctggctcagc atcgactcgg cgcggccgat cgcgcggcgg tcgccgttgc gggcggcgcg
     2521 caaggcgtcg gcgatcctcc gcacgggaaa acgatcgggg tcgagcccgt cgatggacgc
     2581 gctgttgagc aggccgagca tcagctgcgc cgccgctccg gattcgggcg ccagccacaa
     2641 cgggcgcccg tctcgagcgc ggtagaattc ggcgaccccg ccgcggctgt cggagaagcc
     2701 ggcgggctgc gccgtcccgg cggcggtcgc cgcggtcgcg gcgaaggaag gaagcggcgc
     2761 cgcggccagc atcacggccg ccgcgacacc gcaggtttgt gaaaaaatgc gcgccggacg
     2821 aggtccggcg cgcttgatac gcatactgat cacttcaacg accgattacc cgcgctcacc
     2881 gctgcgcatc ggagccgaat aggtcggcgc cggagcggtg gtggtcggtg cgccggcata
     2941 atattggccg ttgtagacat agccgtcgac ccggccgtca ttatcgcggt cggcccagat
     3001 cgctccggcg acaccgccga cggcggcgcc gatcaccgcg ccttcgagga cgctgaggcc
     3061 cgggatcacc gcacccgcgg ccgctccgag ggcagcgccg ccgagcgcgc cggtggcgac
     3121 gcggcgaagg gtcgggtcat agccctgcgg ggtgccggca taatattgcc cgttctggac
     3181 ataaccgtcg acatagccgt cgttgttgcg atcggcccag acggcattcg caaggccgcc
     3241 gatcgcggcg ccaaccgccg cgccctcgat catgctgacg ccggggacaa ccgcgccgat
     3301 cgcggctccg ccggcagcgc cgtacgccgc gccttccgcg acgtcacccg ccatgcggtc
     3361 gttggtggcg catgcgccga gcgagaccga actcgccagg acaatggcca gaattgattt
     3421 tttcatcatg tttctcccga ttattgagct tgcat
//
LOCUS       DASTZO010000166        11615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1084370,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000166 DASTZO010000000
VERSION     DASTZO010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 11615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11615
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084370"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VFU20_04620"
     CDS             complement(<1..717)
                     /locus_tag="VFU20_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU5285780.1"
                     /translation="MARTKSAASAPPAIAAWLVHAFTASGAVLALLALLAIEQQRWTL
                     ALAWLALSGVVDGVDGSLARWARVKERAPSIDGDTLDLVIDYLTYVFVPALFIWRAEL
                     VPPGLAPWLAAAILVSSLYVFARTDMKTDDGYFRGFPALWSVVAFYLFVLQPGAGIGA
                     AVVLLLVAMTFAPIHFVHPFRVRDYGKWLPAVAIAWIAATCALLWPGWSDTARTGIAT
                     ASVATAAALVGMGLLRSLRGG"
     gene            819..2273
                     /locus_tag="VFU20_04625"
     CDS             819..2273
                     /locus_tag="VFU20_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU5285781.1"
                     /translation="MLDPGRNCWRIERADRAALIVDACDYFHHARNAMLAAQEQILLI
                     GWDFDTRILLDHDCRDEAPATLGAFISWLARHRPQVKVHILRWDWGATKLLARGTTVL
                     RLVNWARTPQIEFKLDGAHPAGASHHQKIVVIDDRLAFCGGIDMTATRWDTREHRDGD
                     TRRERPTTGRHYKPWHDATMAVDGAAAQALGELARERWKTAGGKPIAAPSADGDPWPD
                     GLEPQFEGAEVAIARTRAEHEGVAEVREIEALYADMIARARRYIYFENQFFASRVVGE
                     AIAKRLQEAEGPEFVIVNPKSAEGWIEEEVMGPARARLLQELWKADRHNRLRVYTPVT
                     EGGCDIYVHSKIGIVDGELLRVGSANLNNRSMGLDSECDLLIDCGRAANAGVSSKIGA
                     ILCDLLAEHLGVPPDDVQDRLSASGSLIRTVEELCGSGRTLVPLKVDQPNALEAEIAG
                     REALDPERPGEYFEPIARRGLLGRLRFRLPFARR"
     gene            2384..3418
                     /locus_tag="VFU20_04630"
     CDS             2384..3418
                     /locus_tag="VFU20_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5285782.1"
                     /translation="MNQIKFTCAAALLAAVSMPGIAEARSMRAVGSSTVYPFSKAVAE
                     RVARANPKLGTPIIESTGTGAGFKLFCAGVGARFPDVSNASRRIKASEAKLCAANGVK
                     QVTEIQIGVDGLAAATAKSSALNSLTQRDIYLAIAKTPFGKPNKAKTWKDVNSRLPAI
                     PIRVYGPPTTSGTRDSLHELILTPPCEANPGMAALKKSDEARFKAVCTGIRSDGAYIE
                     AGENDNLIVQKLAANPNTVGFFGYSFLEENVSRLKGIAINGVQPNYQTISSFKYPGAR
                     PLYIYVKNAHAKAIPAIGAFVAEYTKESAMGPKGYLVRHGLIAAPPNVRARSQVAARN
                     LVPLNLASIK"
     gene            3494..4885
                     /locus_tag="VFU20_04635"
     CDS             3494..4885
                     /locus_tag="VFU20_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HEU5285783.1"
                     /translation="MIRKTFIAALLCGTAASLSAPAAAQELPAAQQQPEPTEPDPSDA
                     TADAAIAAATPTDDAQAKIELLEAQVEALQEALEGIKAQMAATTPSWKGAPQLIDKEA
                     GWSFKPRGRLMYDFATVGSPDGFDNAGLGFSNEVRRARLGVEGTVPGGFGYKFELDFA
                     AGDVEFADAFLSYSKGPLEFIVGHHNNFQSLDELTSSLHSSFIERAAFTDAFGFERRV
                     GASVQYAKGDFIAQAGAFTDSISDLNSVGDDNNSRGVDARLVFAPKMGSNQLHFGGSA
                     HYRDINDAATSVRYRQRPAVHTTDIRFVDTGNITGAETETGYGLEAAGIFGPFHLASE
                     AYWQKVGRDGFSDPGFFGAYVEGGYFLTGESRGYKGGKFDRVKVKKPFSDGGWGSLGI
                     NLRWDYLDLIDAGILGGKQNAYQASLNWKPTDYLLFGLNYAHLVYTDAAVAADGDRDY
                     GVDMAAVRAQVDF"
     gene            5005..6006
                     /locus_tag="VFU20_04640"
     CDS             5005..6006
                     /locus_tag="VFU20_04640"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5285784.1"
                     /translation="MANAGMRPFLEALDEPAMVLEGGVIRFANEAARRLLGGAIEGND
                     VRLAIRHPQALEHILPGEPGDVDITGIGQFGRPWTLSIRKIDGRSLLVRLIDRSAAIA
                     AEKMRVDFVANASHELRTPLATILGYSETLADQGDLEPEVRVTFGSTIRTEAERMLRI
                     IEDLMSLSRIEAGRFVTPSERVAIGEVAASAVENAGRIAGADQCQVALDVEDKLPPVL
                     GDRAQLLQLLDNLLSNAIRYGCERGGCAVRVAAAGDDGFVRLTVGDNGPGIPREHLRR
                     VTERFYRVDAARSRESGGTGLGLAIVKHIVERHRGTLDIRSTLGEGTEVMIRLPVAP"
     gene            6107..7141
                     /locus_tag="VFU20_04645"
     CDS             6107..7141
                     /locus_tag="VFU20_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5285785.1"
                     /translation="MTRTLPLIAAAALLAACGSNTSGGGSGQQLKIVGSSTVYPFTRA
                     VAEEFQRANRGTSVIVESTGTGAGLKLFCSGVGADFPDLTNASRRIKASELEDCQKNG
                     VKQVIEVPVGIDGLAVIESAQARPMKLTLRDLYAALAATPFGKPQTAQTWKDVNPALP
                     AVKIRVLGPPPTSGTRDSLAELFLEKGCNTDPAMKALKDSDSDKHKETCTKIREDGAF
                     VEAGENDNLLVQKVATDPTALGVLGYSFLEENADKVRAVELNGSLPTAETITSLSYPG
                     ARIVYIYAKGEHMQAKPAIRAFLAAYAKAWGKGGPLQRIGLVPLAGADADAANSQATA
                     LKPLDAAALK"
     gene            7161..8558
                     /gene="pstC"
                     /locus_tag="VFU20_04650"
     CDS             7161..8558
                     /gene="pstC"
                     /locus_tag="VFU20_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU5285786.1"
                     /translation="MSLAIALAAVLAVAAAAGWLGYSRATRLRTARRASGGGRLHSLP
                     VYHGAYAALWAALPALLLLALWAPVQSNLVDQAVLASPEGRALPQFDMQRDTILAEAR
                     EMARGDREAGFNPESSTLVPRFAEAQSRYSTVGGAVAILLALAAAAFALRRTSPQFRA
                     RTGVERWLMAVLVAASLIAILTTLGIVLSLMFESLRFFELVPPGEFLFGTQWSPQTAL
                     RADQAGSSGAFGSVPLFWGTIFIGAIIAMIVAIPLGLMSAIYLTQYAHPQVRAWMKPL
                     LEILAGVPTVVYGYFAALTVAPAVRDLGLSLGITSASSESALAAGLVMGIMIIPFISS
                     MSDDSIAAVPQAMRDGSLAMGATKSETIRKVVLPAALPGVVGGILLAVSRAIGETMIV
                     VMAAGLAANLTANPFASVTTVTTQIVQLLTGDQEFESAKTLAAFALGLALFLITLVLN
                     LIALGVVRRYREAYE"
     gene            8551..9801
                     /gene="pstA"
                     /locus_tag="VFU20_04655"
     CDS             8551..9801
                     /gene="pstA"
                     /locus_tag="VFU20_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU5285787.1"
                     /translation="MSSPVQTLPGRTADARLRKRYAAERRFRLAGLFAVALSAAFLAF
                     LLVTMAWKGLSGFTHFEAAVTIDFPRSDLILDPAALKGPDAQDTVAGADLQSVLMQSA
                     GAAYGDDAAELFGSAAARSLGRQLIADPDLLNRRATIWLPVGSAIDIAAKQDSDPASE
                     RLVARLEAKDSLRRTVNMDFLTQSDATDPSAVGVWGALKGSFLTIVVTILLAFPIGVL
                     AAVYLEEFATRNRWTDAIEVSINNLAAVPSIIFGLLGLAVFLNFMHLPRSAPLVGGLT
                     LALMTMPVIVIAGRNAIKSVPPSIRDAALGVGASKMQVVFHHVLPLALPGILTGTIIG
                     MARALGETAPLLMIGMRAFIAAPPTGITDPATVLPVQIFLWSDEVDRGFVEKTSAAII
                     VLLLFMLLMNGLAIYLRNRFERRW"
     gene            10008..10754
                     /gene="pstB"
                     /locus_tag="VFU20_04660"
     CDS             10008..10754
                     /gene="pstB"
                     /locus_tag="VFU20_04660"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEU5285788.1"
                     /translation="MRAREVSVSYGDKQALNKVSIDIHDDRVTAFIGPSGCGKSTFLR
                     CLNRMNDTIPTARVTGRIELDGEDIHSSAMDVVQLRARVGMVFQKPNPFPKSIFENVA
                     YGPRIHGLGSSKAELEQIVEKSLKRAGLWDEVKDRLNESGTALSGGQQQRLCIARAIA
                     VDPEVILMDEPCSALDPIATAKIEELIHELRGRYAIAIVTHNMQQAARVSQRTAFFHL
                     GELIEYGKTSEIFTNPRQQRTQDYITGRYG"
     gene            10769..11464
                     /gene="phoU"
                     /locus_tag="VFU20_04665"
     CDS             10769..11464
                     /gene="phoU"
                     /locus_tag="VFU20_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU5285789.1"
                     /translation="MQQQTSGHTLKAFDQDIDRLRALISQMGGLAEHAIGEAMRCLIQ
                     RDVEGALKVVENDKKLDALEVETERRAVQLIALRAPMAGDLRDVVAALKISGVVERIG
                     DYAKNIAKRVPLLENSTKIEPVSLLPEMARIATGMVHDVLDAFVQRDAQVAVQVCQRD
                     DAVDDFYDSIFRTLLTHMMENPHNIGHATHLLFVAKNLERVGDHATNIAEMVYYAATG
                     QHMAELARDESNG"
     gene            11467..>11615
                     /locus_tag="VFU20_04670"
     CDS             11467..>11615
                     /locus_tag="VFU20_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEU5285790.1"
                     /translation="MGRNGSKRLLLVEDDRALAELVTFHFDRAGYAVTRTGDGEEALI
                     LAEEI"
BASE COUNT         1928 a         4072 c         3894 g         1721 t
ORIGIN      
        1 gccgccccgc aggctgcgca gcaggcccat tccgaccaag gccgcggcgg tggcgacgga
       61 ggcggttgcg attccggttc gcgccgtgtc gctccagccg ggccacagca gggcgcaggt
      121 cgccgcgatc caggcgatcg ccaccgccgg cagccatttg ccatagtcgc ggacccggaa
      181 cgggtggacg aagtggatcg gggcgaaggt catggcgacc agcagcagca cgaccgcggc
      241 gccgatcccg gctccgggct ggaggacgaa caggtagaag gcgaccacgc tccacagcgc
      301 cgggaagccg cggaaatagc cgtcgtcggt cttcatgtcg gtgcgcgcga agacgtagag
      361 cgaggaaacc aggatcgcgg ccgcgagcca cggcgcaagg ccgggcggca ccagctccgc
      421 ccgccagatg aacagcgccg ggacgaacac ataggtcaaa taatcgatca ccaggtcgag
      481 cgtgtcgccg tcgatactgg gcgcccgctc cttgacccgc gcccagcgcg cgagcgagcc
      541 gtcgacgccg tcgacgaccc ccgacagcgc cagccacgcc agggccaggg tccagcgctg
      601 ctgctcgatc gccagcagcg cgagcagcgc cagcaccgcc ccgctggcgg tgaacgcgtg
      661 caccagccag gcggcgatcg ccggcggagc ggacgcggcg gatttggtgc gggccattga
      721 tcggcggacg gtaatgcaag tcggccggtt ccggaaattg gcgttcgcgg cggaccacgg
      781 tatggccgcc cgggtgcgcg taccgacgat gatccctggt gcttgatccg ggccgcaatt
      841 gctggcggat cgagcgggcg gaccgggccg cgctgatcgt cgacgcgtgc gattatttcc
      901 accacgcgcg caacgcgatg ctcgcggcgc aagagcagat cctgctgatc ggctgggact
      961 tcgacactcg aatcctgctc gaccatgatt gccgcgacga ggcgccggcg acgctcggcg
     1021 cgttcatctc ctggctcgcc aggcaccggc cgcaggtcaa ggtgcatatc ctgagatggg
     1081 actggggcgc gaccaaactg ctcgcccgcg gcacgaccgt gctccggctg gtcaactggg
     1141 cgcgcacccc gcagatcgag ttcaagctcg acggcgccca tccggccggc gccagccacc
     1201 accagaagat cgtcgtgatc gacgaccggc tcgccttttg cggcggcatc gacatgaccg
     1261 cgacccgttg ggacacgcgc gagcatcgcg acggcgacac gaggcgcgag cgcccgacca
     1321 ccggccggca ctacaagccc tggcacgacg cgacgatggc ggtcgacggg gctgcggcgc
     1381 aggccctcgg cgagctcgcc cgcgagcgct ggaagaccgc cggcggaaag ccgatcgccg
     1441 ccccgagcgc cgacggcgac ccgtggcccg acgggctcga gccgcagttc gagggcgccg
     1501 aggtggccat cgcgcgcacc cgcgccgagc atgagggcgt ggccgaggtc cgcgagatcg
     1561 aggcgctgta cgccgacatg atcgcgcggg cccgccgcta catctatttc gagaaccagt
     1621 tcttcgcctc gcgcgtcgtc ggcgaagcga tcgccaagcg gctgcaggaa gccgaggggc
     1681 ccgagttcgt catcgtcaac ccgaagagcg ccgaagggtg gatcgaggag gaggtaatgg
     1741 gcccagcccg ggccaggctc ctccaggagc tgtggaaagc cgaccgccac aaccggctgc
     1801 gggtctacac tccggtcacc gaaggcggct gcgacatcta cgtccactcg aagatcggga
     1861 tcgtcgacgg cgagctgctg cgcgtcggct cggccaatct caacaaccgc tcgatgggcc
     1921 tcgacagcga gtgcgacctg ctgatcgact gcggccgggc ggcgaatgcg ggtgtgtcgt
     1981 cgaagatcgg cgcgatcctg tgcgacctgc tcgccgagca tctgggggtg ccgccggacg
     2041 atgtgcaaga ccggctctct gcgtccgggt cgctaatccg aacggtcgag gagctgtgcg
     2101 gaagcggccg gacgctggtg ccgctcaagg tcgaccagcc gaacgcgctc gaggcggaga
     2161 tcgccgggcg cgaggcgctc gatcccgagc ggccgggcga atatttcgag ccgatcgccc
     2221 gccgcggcct gctcggccgg ctgcgcttcc gcctgccgtt cgcacggcgc tagccgacgg
     2281 ctgccgctgt cacgggaatg tcatagccga gtcattcgcg cgtagctttg gggtcctaat
     2341 tccgagtcgt ggacggcgat gggccggacc aaggggactc gtgatgaacc agatcaaatt
     2401 cacttgcgcg gcggcgcttc tggccgcggt ttcgatgccc ggaatcgccg aggcccgctc
     2461 gatgcgggcg gtcggctcct cgaccgtcta tcccttctcc aaggcggtcg ccgagcgagt
     2521 cgcccgggcc aacccgaagc tcggcacgcc gatcatcgaa tcgaccggca ccggggcggg
     2581 cttcaagctg ttctgcgccg gagtcggggc gcgtttcccc gacgtgtcga acgcgtcgcg
     2641 gcggatcaag gcgtccgagg ccaagctgtg cgcggccaac ggggtcaagc aggtcaccga
     2701 gatccagatc ggggtcgacg ggctcgccgc cgccaccgcc aagtcgagcg ccctcaactc
     2761 gctcacccag cgcgacatct acctcgccat cgccaagacc ccgttcggca agccgaacaa
     2821 ggccaagacc tggaaggacg tcaactccag gctgccggcg atcccgatcc gggtctacgg
     2881 cccgccgacc accagcggca cccgcgattc gctgcacgag ctgatcctga ccccgccgtg
     2941 cgaggccaat cccggcatgg cggcgctgaa gaagtcggac gaagccaggt tcaaggcggt
     3001 gtgcaccggg atccgctcgg acggcgccta tatcgaggcc ggcgagaacg acaatctcat
     3061 cgtccagaag ctcgccgcca accccaatac ggtcggcttc ttcggctaca gcttcctcga
     3121 ggagaacgtg agccgcctga aaggcatcgc gatcaacggc gtccagccca attaccagac
     3181 gatcagcagc ttcaaatatc cgggcgcccg gccgctctat atctacgtca agaacgcgca
     3241 cgccaaggcg atcccggcaa tcggggcgtt cgtcgccgaa tataccaagg aaagcgcgat
     3301 gggccccaag ggctatctgg tccggcacgg cctgatcgcc gctccgccca acgtgcgcgc
     3361 tcgaagccag gtcgcggccc gcaacctggt gccgctcaac ctcgccagca tcaaatagct
     3421 cgcgccgtcg caacgcattg aatcggggag cgccgcccgc cgcggcgggc gctcggggaa
     3481 aggaaagact ggaatgatcc gcaagacctt catcgccgcg ctgctgtgcg ggaccgcagc
     3541 gagtttgtcc gcgcccgcgg cagcgcagga gctgccggcc gctcagcaac agcccgagcc
     3601 gaccgagccc gatccgtcgg acgcgacggc cgacgcggcg atcgcggcgg cgaccccgac
     3661 cgacgacgcc caggccaaga tcgagctgct cgaggcgcag gtcgaggcgc tccaggaggc
     3721 gctcgaggga atcaaggcgc agatggcggc gaccacgccg tcgtggaaag gcgctccgca
     3781 gctgatcgac aaggaggccg gctggtcgtt caagccgcgc gggcggctga tgtacgactt
     3841 cgcgacggtc ggctccccgg acggcttcga caatgccggc ctgggcttct ccaatgaggt
     3901 gcgccgcgcc cgcctgggag ttgaaggcac cgtcccgggc ggcttcggct acaagttcga
     3961 gctcgatttc gcggccgggg acgtggagtt cgccgacgcc ttcctgtcgt acagcaaggg
     4021 cccgctcgag ttcatcgtcg gccatcacaa caatttccag tcgctcgacg agctgaccag
     4081 ctcgctccac agctcgttca tcgagcgcgc ggccttcacc gatgccttcg gcttcgagcg
     4141 gcgggtgggg gcctcggtcc aatacgccaa gggcgacttt atcgcccagg caggagcgtt
     4201 caccgacagc atcagcgacc tgaactcggt cggcgacgac aacaacagcc gcggcgtcga
     4261 cgcccgcctg gtcttcgctc ccaagatggg ctccaaccag ctgcacttcg gcggctcggc
     4321 gcattatcgc gacatcaacg acgccgcgac cagcgtccgc taccgccagc gccccgcggt
     4381 tcacacgacc gacatccgct tcgtcgacac cggcaacatc accggcgccg aaaccgagac
     4441 cggctacggg ctggaggcgg ccggcatctt cgggcccttc catttggcca gcgaagccta
     4501 ttggcaaaag gtcggccgcg acggcttttc cgaccccggc ttcttcggcg cctatgtcga
     4561 aggcggctat ttcctgaccg gcgaaagccg cggctacaag ggcggcaagt tcgaccgcgt
     4621 caaggtcaag aagccgttca gcgacggcgg ctggggctcg ctcggcatta acctgcgctg
     4681 ggattatctc gacttgatcg acgccgggat cctcggcggc aagcagaacg cctaccaggc
     4741 ttcgctcaac tggaagccca ccgactatct cctgttcggc ctcaattacg cgcatctggt
     4801 ttacaccgac gcggcggtcg ccgccgacgg cgaccgcgac tatggcgtcg atatggctgc
     4861 ggtgcgcgct caggtcgact tctaagcctt ttcgtccctc cccgacgcaa aaggcgcccc
     4921 ctcgttcgcc tggacgaggg ggttttgcgt cggccgcgcc ggcccggatg gccgcgggcg
     4981 atcgaccggc ctaaaggcgt gaccatggcg aacgcgggca tgcgaccctt cctcgaagcg
     5041 ctcgacgagc cggcgatggt gctcgagggc ggcgtcatcc ggttcgccaa cgaggccgcg
     5101 cgcaggcttc tcggcggcgc catcgaaggc aatgacgtcc gcctcgcgat ccgccatccc
     5161 caggcgctcg agcatatcct cccgggcgag ccgggcgacg tcgacattac cggcatcggc
     5221 cagttcggcc ggccgtggac cctgtccatc cgcaagatcg acggccgctc gctgctcgtc
     5281 cgcctgatcg accggtcggc ggcaatcgcg gccgagaaga tgcgcgtcga tttcgtcgcc
     5341 aacgccagcc acgagctgcg caccccgctg gcgacgatcc tgggctattc ggaaacgctc
     5401 gccgaccagg gcgacctcga gcccgaggtc cgggtgacgt tcggctcgac catccgcacc
     5461 gaagccgagc gcatgctccg gatcatcgag gatttgatga gcctgtcgcg gatcgaggcg
     5521 ggccgattcg tcaccccgtc cgagcgggtt gcgatcggcg aggtggcggc gagcgcggtc
     5581 gagaacgccg gccggatcgc cggcgccgac cagtgccagg ttgcgctcga cgtcgaggac
     5641 aagctgccgc cggtcctcgg cgaccgcgcc cagctgctgc aattgctcga caatctgctg
     5701 agcaacgcga ttcgctacgg gtgcgagcgc ggcggctgcg cggtccgagt cgcggcggcc
     5761 ggcgacgacg gcttcgtccg gctgacggtc ggcgacaacg gtccgggaat cccgcgcgag
     5821 catttgcgcc gggtgaccga gcgcttctac cgagtcgacg cggcgcgcag ccgcgaatcg
     5881 ggcggcaccg ggctcggcct ggcgatcgtc aagcacatcg tcgagcgcca tcgcggaacg
     5941 ctcgacatcc gcagcacgct cggcgaaggg accgaggtca tgatccgcct gccggtggcg
     6001 ccgtgaggag tcagcgctgt cacaaaagtg taacacgact gtcacgcagc cgtcagatgc
     6061 ggcggctaag gcggcgctcg acaagccttt cgaccatgga gcgaacatga ccaggaccct
     6121 gcctttgatc gccgctgcgg cgctgctcgc ggcatgcggc agcaacactt ccggcggcgg
     6181 ctccggccag cagctcaaga tcgtcggctc ctcgaccgtc tatccgttca cccgggcggt
     6241 cgccgaggaa ttccagcgcg ccaaccgcgg caccagcgtg atcgtcgagt cgaccggcac
     6301 cggcgcgggc ctgaagctgt tctgctcggg cgtcggcgcc gatttccccg acctcaccaa
     6361 cgcgtcgcgg cggatcaagg cgagcgagct cgaggactgc cagaagaacg gcgtcaagca
     6421 agtcatcgag gtgccggtcg ggatcgacgg cctggcggtg atcgaatcgg cccaggcgcg
     6481 accgatgaag ctgaccctgc gtgaccttta cgcggcgctc gccgcgaccc cgttcggcaa
     6541 gccgcagacc gcgcaaacct ggaaggatgt caacccggcg cttccggcgg tcaagattcg
     6601 cgtgctcggc ccgccgccga cctccggcac ccgcgacagc ctcgcggagc tgttcctcga
     6661 gaaaggctgc aacaccgatc cggcgatgaa ggcgctcaag gacagcgatt ccgacaagca
     6721 caaggaaacc tgcaccaaga tccgcgagga cggcgccttc gtcgaggccg gggagaacga
     6781 caatctgctc gtccagaagg tcgcgaccga cccgaccgcg ctcggagtgc tcggctacag
     6841 cttcctcgag gagaacgccg acaaggtgcg cgcggtggag ctcaacggct cgcttccgac
     6901 cgccgagaca atcaccagcc tgtcctaccc gggcgcgcgc atcgtctata tctatgccaa
     6961 gggcgagcac atgcaggcca agccggcgat ccgcgcgttc ctcgccgctt acgccaaggc
     7021 ctggggcaag ggcggcccgc tgcagcgcat cggcctggtg ccgctggccg gcgccgacgc
     7081 ggacgcggcc aacagccaag ccaccgcgct caagccgctc gacgccgcgg cgctgaagta
     7141 agcgaacggc ggccgccgca atgagcctcg ccattgcgct cgccgccgtg ctcgccgttg
     7201 ccgcggcggc gggatggctc ggctattcgc gggcgacccg cctgcgcacc gcccgccgag
     7261 cttccggcgg cggccggctg cactccttgc ccgtctatca cggcgcctat gcggccctgt
     7321 gggcggcgct gccggcgctg ttgctgctgg cgctgtgggc gccggtgcag tcgaacctgg
     7381 tcgaccaggc ggttctggcg agccccgagg gccgcgcgct gccccagttc gacatgcagc
     7441 gcgacaccat cctcgccgaa gcgcgcgaga tggcgcgcgg cgaccgcgag gccggcttca
     7501 accccgaatc ctcgaccctc gtgccgcgct ttgccgaggc ccagagccgc tattcgacgg
     7561 tcggcggagc ggtcgcgatc ctgctcgcgc tcgcagccgc cgcgttcgct ctgcgccgca
     7621 cctcgccgca gttccgggcc cggacggggg tcgagcgatg gctgatggcg gtgctggtcg
     7681 ccgcctcgct gatcgcgatc ctgacgactc tcggcatcgt cctgtcgctg atgttcgaaa
     7741 gcctgcgctt tttcgaactg gtgccgccgg gcgagttcct gttcggcacc cagtggagcc
     7801 cgcagaccgc gcttcgcgcc gaccaggcgg gctcgtcggg cgcgttcggc tcggtgccgc
     7861 tgttctgggg gacgatcttc atcggcgcga tcatcgccat gatcgtcgcc atcccgctgg
     7921 gcctgatgag cgccatctat ctgacccaat acgcccaccc gcaggtgcgc gcctggatga
     7981 agccgctgct cgagatcctc gccggcgtcc cgaccgtggt ctacggctat ttcgcagcgc
     8041 tgacggtggc gccggcggtg cgcgacctcg gcctgtcgct cggaatcacg tcggcgagca
     8101 gcgaaagcgc gctcgccgcg ggcctcgtga tgggcatcat gatcatcccg ttcatcagct
     8161 cgatgtccga cgattcgatc gccgcggtgc cccaagccat gcgcgacggg tcgctggcga
     8221 tgggcgcgac caagtccgag accatccgca aggtggtgct cccggccgcg ctcccggggg
     8281 tggttggcgg aatcctgctc gcggtcagcc gggcgatcgg cgagacgatg atcgtggtga
     8341 tggccgcggg cctggccgcc aatctgaccg ccaacccgtt cgccagcgtc accaccgtca
     8401 ccacccagat cgtccagctg ctgaccggcg accaggagtt cgagagcgcc aagacgctcg
     8461 ccgccttcgc gctcgggctg gcgctgttcc tgatcaccct ggtcctcaac ctgatcgctc
     8521 tcggagtcgt ccggcgctat cgggaggcct atgagtagcc cggtccagac gctgcccggg
     8581 cggaccgccg acgcgcgcct gcgcaagcgc tacgcggccg agcgccggtt ccggctggcc
     8641 ggcctgttcg cggtcgctct gtcggcggcg ttcctcgcct tcctgctggt caccatggcg
     8701 tggaagggcc tcagcggctt tacccatttc gaggccgcgg tgacgatcga tttcccgcgc
     8761 tcggacctga ttctcgatcc cgccgcgctg aagggcccgg acgcccagga cacggtcgcc
     8821 ggagccgacc tgcaaagcgt gcttatgcag tcggcgggcg ctgcctacgg cgacgatgcg
     8881 gccgagctgt tcggaagcgc cgcggcgcgc tcgctcggcc gccagctgat cgccgacccg
     8941 gacctgctca accgccgggc gacgatctgg ctgccggtcg ggagcgcgat cgacatcgcc
     9001 gccaagcagg acagcgatcc ggccagcgag cgcctggtcg cccggctcga ggcgaaggat
     9061 tcgctgcgcc gcaccgtcaa catggatttc ctcacccagt ccgacgcgac cgacccgtcc
     9121 gcggtcggag tctggggagc gctcaagggc agcttcctga ccatcgtcgt gacgatcctg
     9181 ctcgctttcc cgatcggagt gctcgccgcc gtctatctgg aggagttcgc gacccgcaac
     9241 cgctggaccg acgcgatcga ggtgtcgatc aacaacctcg ccgcggtgcc gtcgatcatc
     9301 ttcggcttgc tcggcctcgc cgtgtttttg aatttcatgc atttgccgcg ctcggcgccc
     9361 ttggtcggcg ggctgaccct ggcgctgatg accatgccgg tgatcgtcat cgccggccgc
     9421 aacgcgatca agtcggtgcc gccgtcgatc cgcgacgccg cgctgggggt cggagcgtcg
     9481 aagatgcagg tcgtgttcca ccacgtgctg ccgctggcgc tgccggggat cctgaccggg
     9541 acgatcatcg gcatggcccg agcgctgggc gaaaccgcgc cgctgctgat gatcggcatg
     9601 cgcgccttca tcgccgctcc gccgaccggg atcaccgacc cggcgaccgt tttgccggtt
     9661 cagattttcc tgtggtcgga cgaggtcgac cgcggcttcg tcgagaagac cagcgcggcg
     9721 atcatcgtgc tgctgctgtt catgctgttg atgaacggcc tggcgatcta tctacgcaac
     9781 cgcttcgagc gccgctggtg aggccgatga aagagaaaat gccactcatg tcgacgacac
     9841 cggtgccgag cccctcggcg gcgatcgccg cggacgccaa ccgcgaaggc ccttcggcga
     9901 cccgcgacga cggtcttggc ccggagcccg acgccctgca ggacgccaac gcagtcgctt
     9961 ccgccggggc cccggtcgcc gagcccgacg ccggcagcac gcccaagatg cgggcgcgcg
    10021 aggtcagcgt gtcctacggc gacaagcagg cgctcaacaa agtctcgatc gacatccacg
    10081 acgaccgggt gaccgccttc atcggcccgt cgggctgcgg caagtcgacc ttcctccgct
    10141 gcctcaaccg gatgaacgat accattccga ccgcgcgcgt gaccgggcgg atcgagctcg
    10201 acggcgagga catccacagc tcggcgatgg acgtggtgca gctgcgcgcc cgcgtcggaa
    10261 tggtgttcca gaagcccaac ccgttcccca agtcgatctt cgagaatgtc gcctacggcc
    10321 ctcgcatcca cggcctcggc agctcgaagg cggagctcga gcagatcgtc gagaaatcgc
    10381 tcaagcgcgc cgggctgtgg gacgaggtca aggaccgttt gaacgagagc ggcaccgccc
    10441 tgtccggcgg ccagcagcag cggctgtgca tcgcccgggc gatcgccgtc gaccccgaag
    10501 tgatcctgat ggacgagccg tgctcggcgc tcgacccgat cgccaccgcc aagatcgagg
    10561 agctgatcca cgagcttcgc ggccgctacg cgatcgccat cgtcacccac aacatgcagc
    10621 aggcggcgcg ggtgtcgcag cggaccgcct tcttccacct cggcgagctg atcgaatacg
    10681 gcaagaccag cgagattttc accaatccgc ggcagcagcg cacccaggat tacatcaccg
    10741 gccgctatgg ctgatcggag cgaagtcaat gcaacagcag accagcgggc acaccctcaa
    10801 ggcgttcgac caggacatcg accggctgcg cgcgctcatc agccagatgg ggggcctggc
    10861 cgagcatgcg atcggcgagg cgatgcgctg cctcatccag cgcgacgtcg agggcgccct
    10921 caaggtcgtc gagaacgaca agaagctcga cgcgctcgag gtcgagaccg agcgtcgggc
    10981 ggtgcagctg atcgcgctgc gcgcgccgat ggcgggcgac cttcgcgacg tcgtcgcggc
    11041 gttgaagatc tcgggcgtcg tcgagcgcat cggcgattat gccaagaaca tcgccaagcg
    11101 cgtgccgctg ctcgagaatt cgaccaagat cgagccggtg tcgctgcttc cggagatggc
    11161 gcggatcgcg accggaatgg tccacgacgt gctcgacgcg ttcgtccagc gcgacgccca
    11221 ggtcgcggtc caggtgtgcc agcgcgacga tgcggtcgac gatttctacg attcgatctt
    11281 ccgcaccctg ctcacccaca tgatggaaaa cccgcacaac atcggccacg cgacccacct
    11341 gctgttcgtc gccaagaacc tcgagcgggt cggcgaccat gcgaccaaca tcgccgagat
    11401 ggtctattat gcggcgaccg gccagcacat ggccgaactc gcccgcgacg agagcaacgg
    11461 ctgacgatgg gccggaacgg ctccaagcgc ctgctgctgg tcgaggacga ccgggcgctg
    11521 gccgagctgg tgacgttcca cttcgaccgc gccggctatg cggtcacgcg caccggcgat
    11581 ggcgaggaag cgctgatcct ggccgaggag atcaa
//
LOCUS       DASTZO010000167         5862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_588876,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000167 DASTZO010000000
VERSION     DASTZO010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5862
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_588876"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3688
                     /locus_tag="VFU20_04675"
     CDS             <1..3688
                     /locus_tag="VFU20_04675"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEU5285791.1"
                     /translation="LELAPAITGQARSGRVRGRADVRAGRAMVELGVAIDGGGDRIAL
                     SLDAEPDRDRFDVDLRAKSPANGLLPALAGTRRSIDLTVGGDGSWSRWRGRAALDLSG
                     RPAARLALGADSGRYRLAGEIAASQFLKGKLQRLTAPVVRVRGDGRLEDRVLDGQLAL
                     TSRALRAVSVGAIDLGGNRYRRLRVGIDLLRPSALFPNMTGRNVRMAWTLDGPFGRAD
                     YAYRLTSPGVKFDDTGFVDVRAEGRGRLTPWPMRVPLRLSARAITGVGDVAGRMLANA
                     RLEGILTINPKFVRGEGLRLTSNQLKGKVALLIDLVSGRFEILISGGLTRYAISGLGI
                     VDVVTDLKVVPGPGGKGSRVVGTGKAWVRRLDNSFFRDIAGGLPRIETNLERGNDGVV
                     HFTNLELFAPALRLSGAGRRNRDGTFHIVARGRQSKYGALRMTLDGRIERPRVELLLD
                     RPNETLGLTNMRLLLEPTAAGFDYRANGGSRLGPFTSNGRILLPSGGRATVAIAALDV
                     GGSTASGNLRSDPGGFTGTLAISGDLAGELAFAPAGDAQRIEAHLRASGARFPGIFAV
                     RSGRLDGTVILAEERTTLDGVIDARGFEASGISLGRLTANARLVNGSGQVRAAIAGRR
                     GAAFDFTTLANVTPDSIRLTGHGQIERRPLVLRQAAVLTRSGDGRSLAPTSFSFAGGS
                     GTLSGRSGSRPELHAQLRSMPLQILDVAWPGLGLEGSATGRVDYAWKGNRSGRADLRI
                     RGLSRAGLVLASQPIDVGIAAVVGGDQAALRAVAASGGKTIGRAQARFAPLGHGPLVA
                     ELLDARLFAQLRYAGPADTLWRLSGTEVFDLSGPIAIGADIGGRLVDPVIRGSLRTQN
                     ARLESAVTGMVIDQLASDARFSGPQLVFTRIAGRTSGGGAIDGSGRVTFSGGRSLLDL
                     NFTANQALLLNRDDIAARVTGPLRIRSTAEGGLISGQLRLDKGRFQLGRASAAASVPQ
                     LQVRHRGLDPEDVIEVRQLHPWRLDLDVAGRDLEVRGLGIDSRWRTELDIGGTADEPR
                     FTGRADLVRGDYEFAGRNFRLDRGVIRFRGESPPDPLLDIHAEAQVQGIDASVLVRGT
                     GLKPEITFASTPALPQDELLSRILFGTSITNLSAPEAVQLASAVAALQSGSGNLDPIN
                     AVRRAVGLDRLRIVPADVATGQKTALSAGKYITRKLFVEVVTDGQGYSATRVEYQMTR
                     WLSLLSSVSTIGRTSANVRVSKDY"
     gene            3753..5165
                     /locus_tag="VFU20_04680"
     CDS             3753..5165
                     /locus_tag="VFU20_04680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5285792.1"
                     /translation="MKNWNVRKAIVTREEMPEEQRLHQSLSWPHLIALGVGAIVGTGI
                     LTLTGVGAAKAGPAVILSFAIAGIICAFAALAYAEMATMYPASGSAYTYSYIVFGELI
                     AWIVGWSLILEYSLVVSAVAVGWSGYAAGFLASIGAGLPPELVQGPALGGVINLPAIF
                     IIAVVAGLLIYGTRESANVNAFLVAVKLAALALFIAVALPVFNADNFTPFMPYGFPRS
                     GPSGSEVGVMAAAAIIFFAFYGFDAIATAAEETKDPDRDLGIGIVGSMVICVIIYMAV
                     AAVAVGAISYTRIADSPEPLALILREIGQGWAAKILGVSAVVALPTVILAFFFGQSRI
                     FFTMARDGLLPINLARVSSRGTPVRITIFTGIVVAVIAGLFPLADIAALANAGTLTAF
                     IAVAAAMLAMRRRAPDMPRKFTTPWPWLVGVGAILGCAYLFWSLPVQTQTYFLIWNLL
                     GIVLYIVYGSPRAEKARAAA"
     gene            complement(5166..>5862)
                     /locus_tag="VFU20_04685"
     CDS             complement(5166..>5862)
                     /locus_tag="VFU20_04685"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5285793.1"
                     /translation="SHVVELINLSHGGAMVAGDFKAKLWDKVGLVLGDGGEAGEIECA
                     VRWIRDGRYGLEFAHETHVECDSETLDELLRQVIRNSFPGVELKPRPAPPEPAEEPEQ
                     KRGDVRHPLIWNGILHHDYDWEVVRLRNISRLGALIECPANLPAGVTVYLDLAEAGRL
                     AASVSWSRGNQAGLAFAEPFDVRSLARTAPDVAAQQGYRPPFDEVGQSDQSPWAPQWG
                     RLSVDELGKNLGG"
BASE COUNT          782 a         2110 c         2085 g          885 t
ORIGIN      
        1 gctcgagctc gcgcccgcga tcaccgggca ggcgcgcagc ggccgcgtgc gcgggcgtgc
       61 ggacgtacgt gcgggcaggg cgatggtcga gcttggagtc gccatcgacg gcggcggcga
      121 ccggatcgcg ctgagcctcg acgcggagcc ggaccgcgac cgcttcgacg tcgacctgcg
      181 cgccaaatcg ccagccaacg gcttgctccc ggcgctggcg ggcacccggc gctcgatcga
      241 cctgacggtc ggcggtgacg gcagctggag ccgctggcgc ggccgggcgg cgctcgacct
      301 ttccggccga ccggccgccc ggctggcgct gggcgccgac tccgggcgct accgcctcgc
      361 gggcgagatc gccgcgtcgc aattcctcaa gggcaagctg cagcggctga cggcgccggt
      421 ggtccgggtg cgcggcgacg gccggctcga ggaccgggtg ctcgacgggc agctggcgct
      481 gacctcgcga gcgctgcggg cggtgagcgt cggagcgatc gacctcggcg gcaaccgcta
      541 ccggcgcctt cgggtcggca tcgacctgct ccggcccagc gccctgttcc ccaacatgac
      601 cggccgcaac gtgcggatgg catggaccct cgacgggccg ttcggccgag ccgactatgc
      661 gtaccggctg acctcgcccg gggtgaagtt cgacgacacc ggcttcgtcg atgtccgtgc
      721 cgagggccgc ggccggctga ctccgtggcc gatgcgggtg ccgctgcggc tgtcggcgag
      781 ggcgatcacc ggcgtcggcg acgtcgccgg gcggatgctc gccaacgccc ggctcgaggg
      841 cattttgacg atcaacccga agttcgtccg cggcgagggg ctgcggctga ccagcaacca
      901 gctgaagggc aaggtcgcgc tgctgatcga tctggtcagc ggccgcttcg aaatcctcat
      961 ttccggcggc ctgacccgct acgcaatctc gggcctcggg atcgtcgacg tcgtcaccga
     1021 cctcaaggtg gtgccggggc cgggcggcaa gggatcgcga gtcgtcggca ccggcaaggc
     1081 ctgggttcgg cggctcgaca acagcttctt ccgcgatatc gccggcggcc tgccgcggat
     1141 cgagaccaat ctcgagcgcg gcaacgacgg cgtcgtccat ttcaccaacc tcgagctgtt
     1201 cgcgcccgcg ctccggctga gcggcgccgg ccgccgcaac cgcgacggca cctttcacat
     1261 cgtcgcgcgc ggccggcagt cgaaatatgg agcgctgcgc atgactctcg acgggcgcat
     1321 cgagcggccg cgggtcgaat tgctgctcga tcgccccaac gagacgctgg ggctcacgaa
     1381 catgcgcctg ctgctcgagc cgaccgcggc cgggttcgat taccgggcca acggcggctc
     1441 gcgcctgggc ccgttcacaa gcaacgggcg gatcctcctg cccagcggcg gccgagcgac
     1501 ggtcgccatc gccgcgctcg acgtcggcgg aagcacggcg agcggcaatc tgcgctccga
     1561 ccccggcggc ttcaccggca ccctggcgat ctccggcgac cttgccggcg agctcgcctt
     1621 cgccccggcc ggcgacgccc agcggatcga agcgcacctc cgggccagcg gagcccgttt
     1681 ccccggcata ttcgcggtcc gctcgggccg gctcgacggc accgtcatcc tcgccgaaga
     1741 gcggaccacc ctggacggcg tgatcgacgc ccgcggcttc gaggcgagcg ggatcagcct
     1801 cggccggctg accgccaatg cgcggctggt caacggcagc ggccaggtcc gggccgcgat
     1861 cgccggccgc cgcggcgccg cgttcgattt caccacgctc gccaacgtca ctcccgacag
     1921 catccggctg accggccacg gccagatcga gcggcggccg ctggtgctcc gccaggccgc
     1981 ggttctgacc cgctcgggcg acggcaggag cctggctccg accagcttca gcttcgccgg
     2041 cggcagcggc actctgtcgg ggcgcagcgg ctcgcggccc gagctgcacg cgcagctgcg
     2101 ctcgatgccg ctgcagatcc tcgacgtcgc ctggcccggg ctcggcctcg aaggctcggc
     2161 gaccggccgg gtcgactatg cgtggaaggg caaccgcagc ggccgggcgg acctgaggat
     2221 ccgcggcctc agccgggcgg ggctggtgct cgcatcgcag ccgatcgacg tcggcatcgc
     2281 cgcggtggtc ggcggcgacc aggcggcgct tcgagcggtg gcggcgagcg gcggcaagac
     2341 cattggccgg gcccaggcgc ggttcgcgcc gctcggccat ggcccgctgg tcgccgaatt
     2401 gctggacgcg cggctgttcg cccagcttcg ctacgccggc ccggccgaca ccttgtggcg
     2461 gctgtcgggg accgaggtgt tcgacctgtc ggggccgatc gcgatcggcg ccgacatcgg
     2521 cggccggctg gtcgacccgg tgatccgcgg ctcgctccgg acgcagaatg cgcggctcga
     2581 aagcgccgtc accgggatgg tcatcgacca gctcgcctcc gacgcgcgat tctccggccc
     2641 gcagctggtg ttcacccgga tcgccggccg gaccagcggc ggcggagcga tcgacgggtc
     2701 gggccgggtg accttctcgg gcgggcgcag cctgctcgac ctgaatttca ccgccaacca
     2761 ggcgctgctc ctcaaccgcg acgacatcgc cgcccgggtc accgggccgc tgcgcatccg
     2821 ctcgaccgcg gaaggcggac tgatctcggg ccagctgcgg ctcgacaagg gccgcttcca
     2881 gctcggccgc gcgagcgccg ccgcgtcggt cccccagctc caggtgcggc accgcgggct
     2941 cgaccccgag gacgtgatcg aggtccgcca gcttcacccg tggcggctgg acctggacgt
     3001 cgccggccgc gacctcgagg tgcgcgggct cggcatcgac agccgctggc ggaccgagct
     3061 cgacatcggc gggaccgccg acgagccgcg cttcaccggc cgcgcggatt tggtccgcgg
     3121 cgattacgag ttcgcgggcc gcaacttccg cctcgaccgc ggggtgatcc gcttccgcgg
     3181 cgagagcccg cccgacccct tgctcgacat ccacgccgag gcgcaggtgc aggggatcga
     3241 cgccagcgtg ctcgtcaggg gcaccggcct gaagccggag atcaccttcg ccagcacgcc
     3301 cgcgctgccg caggacgagc tattgtcgcg gatcctgttc gggacgtcga tcaccaacct
     3361 gtcggcgccc gaggcggtgc aattggcctc cgcggtcgcg gcgctgcaat cgggctcggg
     3421 caacctcgac ccgatcaacg cggtccggcg cgcggtcggg ctcgaccggc tgcggatcgt
     3481 cccggccgac gtcgcgaccg ggcagaagac ggcgctgtcg gcggggaaat acattactcg
     3541 caagctgttc gtcgaagtcg tcacggacgg tcagggctat tccgcgaccc gggtcgaata
     3601 tcagatgacc cgctggctct ccctgctgtc gtcggtgtcg accatcggcc ggaccagcgc
     3661 caacgtgcgc gtgtcgaaag attactaggg cagcgctgtt gcgccacgcg ggattgcttc
     3721 gctacgcttg gccaacgaac agggggagcg cgatgaagaa ttggaacgtc cgcaaggcga
     3781 tcgtcacccg cgaggaaatg cccgaggagc agcggctcca ccagtcgctg tcgtggccgc
     3841 acctgatcgc cctcggggtc ggggcgatcg tgggcaccgg gatcctgacc ctgaccgggg
     3901 tcggcgccgc caaggccggg ccggcggtga tcctgagctt cgcgattgcc ggcatcatct
     3961 gcgcgttcgc ggcgctcgcc tacgccgaga tggcgaccat gtacccggcc tcgggcagcg
     4021 cctacaccta cagctacatc gtgttcggcg agctgatcgc gtggatcgtc ggctggtcgc
     4081 tgatcctcga atacagcctg gtggtgagcg cggtcgcggt cggctggtcc ggctatgcgg
     4141 ccggcttcct cgccagcatc ggcgccggcc ttccgccgga gctggtccag gggccggccc
     4201 ttggcggagt gatcaacctg ccggcgatct tcatcatcgc cgtcgtcgcc gggctgctga
     4261 tctacgggac ccgcgagagc gccaacgtca acgccttcct ggtggccgtg aagctcgccg
     4321 cgctggcgct gttcatcgcc gtcgccctgc cggtgttcaa cgccgacaat ttcacgccgt
     4381 tcatgcccta cggcttcccg cgcagcgggc cgtccggctc cgaggtcggg gtgatggccg
     4441 cggcggcgat catcttcttc gccttctacg gcttcgacgc gatcgccacc gcggccgagg
     4501 aaaccaagga ccccgaccgc gacctcggca tcggcatcgt cggctcgatg gtcatctgcg
     4561 tgatcatcta catggccgtc gccgcggtcg cggtcggggc gatcagctac acgcgcatcg
     4621 ccgacagccc cgagccgctg gcgctgatcc tgcgcgagat cggccagggc tgggcggcga
     4681 agatcctcgg cgtctcggcg gtcgtggcgc tgccgacggt gatcctcgcc ttcttcttcg
     4741 ggcagagccg gatcttcttc accatggccc gcgacgggct cctgccgatc aatttggcgc
     4801 gggtgtcgag ccgcggaacc ccggtgcgga tcaccatctt caccggaatc gtcgtggcgg
     4861 tcatcgccgg cctgttcccg ctcgctgaca tcgccgcttt ggccaatgcc ggcacgctga
     4921 ccgcgttcat cgcggtggcg gcggcgatgc tggcgatgcg gcggcgagcg cccgacatgc
     4981 cgcgcaagtt cacgacgccg tggccgtggc tggtcggggt cggcgcgatc ctcggctgcg
     5041 cctatctgtt ctggagcctg ccggtgcaga cccagaccta tttcctgatc tggaacctgc
     5101 tcgggatcgt gctttacatc gtctacgggt cgccccgcgc cgaaaaggcc cgcgccgcgg
     5161 cctgatcagc cgccgaggtt cttgccgagc tcgtcgaccg acagccggcc ccattgcggc
     5221 gcccacggcg actggtcgct ctggccgact tcgtcgaacg gcggccggta gccctgctga
     5281 gcggcgacgt cgggcgcggt cctggccagg cttcggacgt cgaacggctc ggcgaaggcg
     5341 aggccggcct ggttgccgcg cgaccagctg acgctggcgg cgaggcgccc cgcctcggcg
     5401 aggtcgagat agacggtgac cccggcgggc agattggccg ggcattcgat cagtgctccg
     5461 agcctcgaga tattgcgcag gcgaacgacc tcccagtcgt agtcgtggtg gaggatgccg
     5521 ttccagatca gggggtggcg gacgtcgccg cgcttctgct cgggctcctc ggccggctcg
     5581 gggggcgccg gccgcggctt cagctcgacg cccgggaagc tgttgcggat tacctggcgg
     5641 agcaattcgt cgagcgtctc ggaatcgcac tcgacatggg tttcgtgggc gaactcgagg
     5701 ccgtagcggc cgtcgcggat ccagcgcacc gcgcactcga tctcgcccgc ctcgccgccg
     5761 tcgccgagca ccaggccgac cttgtcccac agcttcgcct tgaagtcgcc ggcgaccatc
     5821 gcgccgccgt gcgacaggtt gatcagctcg acgacatggc tc
//
LOCUS       DASTZO010000168         2142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_301427,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000168 DASTZO010000000
VERSION     DASTZO010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2142
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301427"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFU20_04690"
     CDS             complement(<1..693)
                     /locus_tag="VFU20_04690"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU5285794.1"
                     /translation="MAAIDTRIVPFSDAAVAEAAGLIRSGQPVAVATETVYGLAADAT
                     DAAAVARVYEAKGRPSFNPLIVHVPDLAAAQAIGEFGPQALELARRHWPGPLTLVVPL
                     RPGAGIAALVTAGLDTVALRVPAHRAMQALLRAAGRPLAAPSANSSGSISPTRAEHVL
                     GSLGGRIALILDDGPCERGIESTIVAATGGRLRLLRPGPIAVDAPAASGEAIEAPGQM
                     QSHYAPSKPLRLD"
     gene            complement(738..1094)
                     /locus_tag="VFU20_04695"
     CDS             complement(738..1094)
                     /locus_tag="VFU20_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5285795.1"
                     /translation="MTLGSQHEKPVYVRLRETIADAILAGRYRDGDPLPSVRAFAAEQ
                     SANPLTVAKAYQGFQDEGLITVRRGVGMFVAPGASARLRESERNRFVKEEWPAVRDRM
                     RRLGIDPASLIARETA"
     gene            complement(1125..1724)
                     /locus_tag="VFU20_04700"
     CDS             complement(1125..1724)
                     /locus_tag="VFU20_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HEU5285796.1"
                     /translation="MLNDRSSILSLLETRRSAKPRELVGPAPGPAEMERILTIAARIP
                     DHGKLSPWRFVTVADDQRDALAALLHRALDKEDPTATPAHHEKAEQFAHNAGALVVLV
                     SAPVEGHKIPVWEQTLSCGAAGMNLLLAAHALGYAGGWVTGWQAYSEHVRAALCEPGE
                     RIAGFIFIGHPGAELVERPRPPLDSVRRAWTPPQDPLEP"
     gene            1849..>2142
                     /locus_tag="VFU20_04705"
     CDS             1849..>2142
                     /locus_tag="VFU20_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285797.1"
                     /translation="MNGLKPVGSWNEGDWIVVITLGVFALFLLGGAWIASRREPEAIG
                     HPKGLYMLFFAEMWERFSYYGMRGLLKLYMVNYLFTTLREPLQGCKELGTCALV"
BASE COUNT          296 a          731 c          797 g          318 t
ORIGIN      
        1 gtcgagccga agcggtttcg acggcgcgta atggctttgc atctgccccg gcgcctcgat
       61 cgcctcgccg ctcgcggcgg gggcgtcgac ggcgatcggc ccgggccgca gcaggcgcag
      121 ccggcccccg gtggccgcga cgatggtcga ttcgatgccc cgctcgcacg ggccgtcgtc
      181 gagaatcagc gcgatccggc cgccgaggct gccgagcaca tgctcggcgc gcgtcgggct
      241 gatcgagccg ctggaattgg ccgacggggc cgcgagcggc cgcccggccg cccgaagcag
      301 cgcctgcatc gcccggtggg cgggcacgcg cagcgcgacg gtgtcgaggc cggcggtgac
      361 cagcgcggcg atgccggcgc cgggcctgag cggaaccacg agggtcagcg gccccggcca
      421 gtgccgccgg gccagctcga gcgcctgcgg gccgaactcg ccgatggcct gggcggcggc
      481 gaggtcggga acgtggacga tcagcgggtt gaagctcggc cgtcccttgg cctcgtagac
      541 ccgcgccacc gcggccgcgt cggtggcgtc ggcggcaagg ccgtagacgg tctcggtggc
      601 caccgcgacc ggctggccgc ttcggatcag ccccgccgcc tcggcgaccg ccgcgtcgga
      661 gaacgggaca atgcgcgtgt cgatggccgc catggcctgc cgctacaggc gcggcgcggg
      721 cccttcaacc gtgggggcta ggcggtttcc cgggcgatca ggctggccgg gtcgatgccg
      781 agccgccgca tgcgatcgcg cacggccggc cattcttcct tgacgaagcg gttgcgctcg
      841 gattcgcgca ggcgcgcgct ggccccgggg gcgacgaaca tgccgacccc gcgcctgacc
      901 gtgatcagcc cttcgtcctg gaaaccctgg taggccttgg cgacggtcag cgggttggcg
      961 gactgctcgg ccgcgaaggc ccggaccgag ggcagggggt cgccgtcgcg gtagcggccg
     1021 gcgaggatcg cgtcggcgat cgtctcgcgc aggcggacgt aaacgggctt ttcgtgctgc
     1081 gaaccgagcg tcatgctgtc ataatacagc agcgcgggaa aaggtcaagg ttcgagaggg
     1141 tcttgcggcg gcgtccacgc ccgccgcacc gagtccagcg gcggccgggg acgctcgacc
     1201 agctcggcgc cgggatggcc gatgaagatg aagcccgcga tgcgttcgcc gggctcgcac
     1261 aaggcggccc ggacatgctc ggaataggcc tgccagccgg tcacccaacc gccggcgtag
     1321 ccgagcgcat gggcggcgag cagcaggttc atccccgccg ctccgcacga caatgtctgt
     1381 tcccagaccg ggatcttgtg gccctcgacc ggagccgaga ccagcaccac cagcgccccg
     1441 gcgttatgcg cgaactgctc ggccttctcg tggtgcgcgg gagtcgccgt cgggtcctcc
     1501 ttgtcgagcg cccggtggag cagcgccgca agcgcgtcgc gctggtcgtc ggcgacggtg
     1561 acgaagcgcc acggcgacag cttgccatgg tcggggatcc gggcggctat ggtcaggatg
     1621 cgctccatct ccgcgggccc gggggccggg ccgaccagct cgcgcggctt ggcggagcgg
     1681 cgggtttcga gcagcgacag gatcgaggaa cggtcgttga gcatggtgcg cccgtctagg
     1741 cgggtcgccg gtttgcaaca atgcgcttca caacggcgtt tcgcccgcta gggtgcgccg
     1801 cctgacgcac gatttggcac cacgatttgg cactctgggg agaggcgcgt gaacggattg
     1861 aagcctgttg gttcgtggaa cgaaggcgac tggatcgtcg tcattaccct cggcgtattc
     1921 gccttgttcc tgctcggcgg agcctggatc gcttcccgcc gcgagcccga ggcgatcggc
     1981 catcccaagg gcctctacat gctgttcttc gccgagatgt gggagcgctt ctcctactac
     2041 ggcatgcgcg ggctgctgaa gctctacatg gtgaactacc tgttcacgac gctccgcgag
     2101 ccgctccagg gctgcaagga gctcggcacg tgcgccctcg tc
//
LOCUS       DASTZO010000169         8154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1189194,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000169 DASTZO010000000
VERSION     DASTZO010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 8154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8154
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189194"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..892
                     /locus_tag="VFU20_04710"
     CDS             <1..892
                     /locus_tag="VFU20_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEU5285798.1"
                     /translation="GLIGIAFAATAMLAMAGMVVALDAYGPVTDNAGGIAEMSGMEDE
                     VRTRTDALDAVGNTTKAVTKGYAIGSAALGALVLFGAYTTDLEHYATDLGVGAGGIDF
                     SLSNPYVVVGLLLGALLPYLFGALGMTAVGRAAGNVVLDVREQFRSNPGIMDGTSRPN
                     YARTVDLVTKAAIKEMIVPSMLPVLSPIVVFFAIAAIAGRDQGFAALGALLLGVIVSG
                     LFVAISMTSGGGAWDNAKKYIEDGNYGGKGSEAHKAAVTGDTVGDPYKDTAGPAVNPM
                     IKITNIVALLLLAALAGGGA"
     gene            915..1874
                     /locus_tag="VFU20_04715"
     CDS             915..1874
                     /locus_tag="VFU20_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEU5285799.1"
                     /translation="MRIHEAGGPEVLRWEPVEVGEPGPGQARLRQHAAALNFIDVYHR
                     TGLYPQPLPFTPGVEGAGVVDSVGPGVQHVKPGDRVAYAGPIGGYAEVRLIDADRLVR
                     LPDDFGFDLAAAMMLQGMTARMLLRSVYPVAAGDMVLVHAAAGGVGLILCQWAAALGA
                     IVIGTVSTDDKAELARAHGCRHPIVYTRQDFVAEVESITAGAGLPVVYDSVGRDTFLK
                     SLDCLRPRGLMVSFGQSSGPIEPFSPGLLQQKGSLFLTRPTLFHYIASRAELEASAAE
                     LFEAVGSGAVRIEVMQRFPLADAAEAHRALEARATTGSTILTV"
     gene            1958..2206
                     /gene="infA"
                     /locus_tag="VFU20_04720"
     CDS             1958..2206
                     /gene="infA"
                     /locus_tag="VFU20_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU5285800.1"
                     /translation="MAKEELLEMRGKVVELLPNAMFRVELENGHEILGHTAGKMRKNR
                     IRVLTGDEVLVELTPYDLTKGRITYRFMPGRAGPPGYP"
     gene            2222..2794
                     /locus_tag="VFU20_04725"
     CDS             2222..2794
                     /locus_tag="VFU20_04725"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018075580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEU5285801.1"
                     /translation="MRLVLASASPRRLDLLARIGIVPDAVVPADVDETVPRGERPRDH
                     ALRLAIEKAEAVAAIEPDALVLAADTVVAVGRRILPKAEDEATLRACMALLSGRRHRV
                     LTGVALAAPGRPTRTRLVETMIAMKRLSAEEIDHYASHGEWLGKAGGYALQGYGEVFV
                     RHIAGSYSNVVGLPLAETRLLLKAAGYPIA"
     gene            2787..3746
                     /locus_tag="VFU20_04730"
     CDS             2787..3746
                     /locus_tag="VFU20_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease"
                     /protein_id="HEU5285802.1"
                     /translation="MPEWWIERGIGETRAGQVEDGAIVEARIELEGVTRAGTILAARL
                     ADVGQAGRNAVARDDRGAEYLLPRAPAGVTEGAALAIEVLREAIPGSEPWKRPLARAT
                     EDEPSPAAPLTGRELAFPSPGRDELAGAGWDDLVEQARSGMVEFAGGTLRISPTPAMT
                     LIDVDGGLAPAELAVAGAAEAARAVRRLGIGGSIGIDLPTLVGKALRLAAAAAIDAHL
                     PQPFERTAVNGFGFVQVVRPRRRPSLLEIWSDGAAAEARTLLRRAALSGSGPRRLVAH
                     PAVVAVLERRPDWLDALARQLGGAVSLRADAARPMSGGDAEPA"
     gene            3730..3915
                     /gene="yacG"
                     /locus_tag="VFU20_04735"
     CDS             3730..3915
                     /gene="yacG"
                     /locus_tag="VFU20_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HEU5285803.1"
                     /translation="MPSPPKAKPCPLCGKPPSQAHAPFCSRGCKDRDLLKWLGEGYRI
                     PGPPADPAELDSEERDG"
     gene            3956..4031
                     /locus_tag="VFU20_04740"
     tRNA            3956..4031
                     /locus_tag="VFU20_04740"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3989..3991,aa:Phe,seq:gaa)
     gene            4051..4707
                     /locus_tag="VFU20_04745"
     CDS             4051..4707
                     /locus_tag="VFU20_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019052362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5285804.1"
                     /translation="MIDLYTAATPNGYKVSVALEELELPYELHWLKLGAGEQKQPWFL
                     AINPNGRIPAIVDRDAGDFAVFESGAILIYLAEKTGRLMPTDMKGRSLVIQWLMFQMG
                     GLGPMMGQANVFHRYLSERIPVAIDRYQRESRRLLEVLDGRLADHEYLAGDYSIADIA
                     NWCWARTHDWSGLDVTGLDNLQRWLAAIAARPAVEKGANIPPRSDEEKSVADARSILV
                     "
     gene            complement(4712..5314)
                     /gene="msrA"
                     /locus_tag="VFU20_04750"
     CDS             complement(4712..5314)
                     /gene="msrA"
                     /locus_tag="VFU20_04750"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU5285805.1"
                     /translation="MKRRIAAAAILCLLAGAAQAAPQRLERAVLAGGCFWGMEAVFEH
                     VKGVRDVVSGYAGGSARDANYAAVSSERTRHAEVVRITYDPRQVSYAQLLRIYATVAH
                     DPTQLNRQGPDVGPSYRSAIFPQSAAQRQLAERFLAQLRASGIYKKPIATRIESGAFY
                     PAEASHQDFVRRNPFHPYVLVHDRPKLAKLRRAYPQLWKA"
     gene            complement(5311..6327)
                     /locus_tag="VFU20_04755"
     CDS             complement(5311..6327)
                     /locus_tag="VFU20_04755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008600882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEU5285806.1"
                     /translation="MKCNGDQVALVAGGAGFIGSHLCDRLVADGATVVCVDNFVTGRR
                     RNIEHLERHPRFRLVEHDVIDPLPLALRGGRIAFTRIYNLACAASPPHYQADPEHTLL
                     SSVVGSRNLLRLAEETGARLLLASTSEVYGEPEVHPQTEEYRGSVSCTGPRACYDEGK
                     RAAETLTFDFIRAGRCEARVARLFNTYGPRMRADDGRVVSNVVCQALEDRDITMYGDG
                     SQTRSFCYVDDTVEGLVRLMGSERAADLPVNIGNPEELSVRELIERVLAMTGSGSSIV
                     RLPLPTDDPRRRKPDISRATELLRWAPRVDLEHGLEATIAWFADDRNRMSEAYEAPLV
                     EAAE"
     gene            6483..6674
                     /locus_tag="VFU20_04760"
     CDS             6483..6674
                     /locus_tag="VFU20_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HEU5285807.1"
                     /translation="MPAKSKAQQRAAGAALSAKRGDTPKSKLKGPARQMASSMSETEL
                     DKMASTKRKGKPERVAKKK"
     gene            complement(6687..7406)
                     /locus_tag="VFU20_04765"
     CDS             complement(6687..7406)
                     /locus_tag="VFU20_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HEU5285808.1"
                     /translation="MTIDETKFFAWLDGELDSSEAAEVEAEVAADARLARMAEQHRAL
                     GARVRGAFDTVAAAPVPERLIEAARPPRSAIVDFGAFRRAKSARGSWLPVPQWPAMAA
                     TLALGIGLGTTLDSNGGSSPVEIRGGRMVAAAALDHALERQLASAGAAGDVRVGLTFR
                     DQAGAICRSFSDQRSSGLACRERGDWRVRGLFAAPEGQGGDYRMAAGADPNLAALIDS
                     TIQGEPFDAAQEQAARERGWR"
     gene            complement(7409..7903)
                     /locus_tag="VFU20_04770"
     CDS             complement(7409..7903)
                     /locus_tag="VFU20_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5285809.1"
                     /translation="MSLAQSFEDELCALLPRLRRFAHGLSRSAADADDLTQMTIERAL
                     RAKAQWQPGTRLDSWLYKIMRNLWIDTVRSRNRQDRVEAPAEEAERLGHDPRDGVEAA
                     IDLHTMMGAMERLPDEQREVVALILIEGFGYREAAELLDLPIGTVSSRLVRGRNALLA
                     LVGE"
     gene            7975..>8154
                     /locus_tag="VFU20_04775"
     CDS             7975..>8154
                     /locus_tag="VFU20_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285810.1"
                     /translation="MSSFVRHALIAAAAAGLVAGAAAAQLLPSVNVSLVGPVVGPVAV
                     PGVPVAGPVLQQILQQ"
BASE COUNT         1177 a         2887 c         2904 g         1186 t
ORIGIN      
        1 gggcctgatc ggaatcgcct tcgccgccac ggcgatgctg gcgatggccg gcatggtcgt
       61 tgcgctcgac gcctatgggc cggtcaccga caatgccggc gggatcgccg aaatgtcggg
      121 gatggaggac gaagtccgca cccgcaccga cgccctcgac gcggtcggca acaccaccaa
      181 ggcggtgacc aagggctatg cgatcggctc ggccgccctt ggcgcgctgg tcctgttcgg
      241 cgcctacacg accgacctcg agcattatgc gaccgacctc ggggtcggcg ccggcgggat
      301 cgatttctcg ctgtccaacc cgtacgtcgt cgtcggcctg ctgctcggcg ccctgctgcc
      361 ctatctgttc ggggcgctgg gcatgaccgc ggtcggccgg gccgccggca acgtcgtgct
      421 cgacgtgcgc gagcagttcc gctccaaccc cggcatcatg gacggcacct cgcggcccaa
      481 ctacgcccgg accgtcgacc tcgtcaccaa ggccgcgatc aaggaaatga tcgtcccgtc
      541 gatgctgccc gtcctcagcc cgatcgtcgt cttcttcgcc attgccgcga tcgccggccg
      601 cgatcagggc ttcgcggcgc tgggcgccct gctgctcggg gtgatcgtct cgggcctgtt
      661 cgtcgccatc tcgatgacca gcggcggcgg cgcctgggac aatgccaaga aatatatcga
      721 ggacggcaat tacggcggca aggggtcgga ggcgcacaag gcggcggtca ccggcgacac
      781 cgtcggcgac ccgtacaagg acaccgccgg cccggccgtc aacccgatga tcaagatcac
      841 caacatcgtc gccttgctgc tgctcgcggc gctcgccggc ggcggcgcct agggcccgcc
      901 cgctggcgca cgcgatccgg atccacgagg cgggcgggcc cgaggtcctg cgctgggaac
      961 cggtcgaggt cggcgagccc gggcccgggc aggcccgcct gcgccagcat gccgcggcgc
     1021 tcaacttcat cgacgtctat caccgcaccg gcctctaccc gcagccgctg ccgttcacgc
     1081 ccggggtcga gggcgccgga gtggtcgaca gcgtcgggcc gggagtccag catgtgaagc
     1141 ccggcgaccg ggtcgcctac gccgggccga tcggcggcta tgccgaggtg cggctgatcg
     1201 acgccgaccg gctggtccgg ctgcccgacg acttcggctt cgacctcgcc gcggcgatga
     1261 tgctgcaggg gatgaccgct cggatgctgc tgcgctctgt ctatccggtc gcggccggcg
     1321 acatggtcct ggtccacgcg gcggccggcg gggtcgggct catcctgtgc cagtgggcgg
     1381 cggccctggg ggcgatcgtc atcggcacgg tgtcgaccga cgacaaggcc gagctcgccc
     1441 gcgcgcacgg ctgccggcac ccgatcgtct acacgcgcca ggacttcgtc gccgaggtcg
     1501 aaagcatcac cgccggcgcc gggctgccgg tggtctatga ttcggtcggc cgcgacacct
     1561 tcctcaagtc gctcgactgc cttcggccgc gcgggctgat ggtcagcttc ggccagtcgt
     1621 cggggccgat cgagcccttc tcgcccggcc ttctgcagca gaagggctcg ctgttcctga
     1681 cccggccgac cctgttccac tacatcgcca gccgcgccga gctcgaggcg tccgcggccg
     1741 agctgttcga ggcggtcggc tcgggcgcgg tgcggatcga ggtcatgcag cgttttccgc
     1801 tcgccgacgc ggccgaggcg caccgggcgc tcgaggcgcg cgcgacgacc ggttcgacca
     1861 tattgaccgt ttgagacgcg cttgcgccgc gcttttcttt tcgcacccgc aagggtaagg
     1921 ctccgcgact ttttcaagat cacgacagcg agggtgaatg gccaaggaag aacttctcga
     1981 gatgcgcggg aaggtggtcg agttgctgcc caacgcgatg ttccgcgtcg agctcgagaa
     2041 cggccatgaa atcctcggcc acaccgccgg caagatgcgc aagaaccgga tccgggtgct
     2101 gaccggcgac gaggtgctgg tcgagctgac gccctacgac ctgaccaagg gccggatcac
     2161 ctatcgcttc atgcccggcc gggcgggacc gccgggctac ccctaacatg cgccaacgag
     2221 gatgagactc gtcctcgcct cggcgagccc gcgccgcctc gacctgctcg cccgcatcgg
     2281 catcgtcccc gacgccgtcg tccccgccga tgtcgacgag accgtcccca ggggcgagcg
     2341 gccgcgcgac catgcgctgc gcctggcgat cgagaaggcc gaggcggtcg cggccatcga
     2401 gcccgatgcg ctggtgctcg ccgccgacac ggtggtcgcg gtcgggcgcc ggatcctgcc
     2461 caaggccgag gacgaagcga ccttgcgcgc gtgcatggcc ttgctgtccg ggcgccgcca
     2521 ccgggtgctg accggggtcg cgctggccgc gcccggccgc ccgacccgga cccggctggt
     2581 cgagacgatg atcgcgatga agcgcctgtc ggccgaggag atcgaccatt atgcaagcca
     2641 cggcgagtgg ctcggcaagg ccggcggcta tgcgctccag ggctatggcg aggtgttcgt
     2701 gcgccacatc gccggaagct attcgaacgt ggtcgggctg ccgctcgccg agacccgatt
     2761 gctgctcaag gccgcgggct accccattgc ctgaatggtg gatcgagcgc gggatcggcg
     2821 agacccgcgc gggccaagtc gaggacggcg cgatcgtcga ggcgcggatc gagctcgagg
     2881 gcgtcactcg cgccgggacg atcctcgctg cccggctggc cgacgtcggg caggccgggc
     2941 gcaacgcggt cgctcgcgac gacagggggg ccgaatatct gctgcccagg gcgccggccg
     3001 gggtgaccga gggcgccgcg ctcgccatcg aggtgctgcg cgaggcgatc cccggaagcg
     3061 aaccgtggaa gcgcccgctc gcccgggcga cggaggacga gccatcgccg gcggcgccgt
     3121 tgaccgggcg cgaactcgcc ttcccgtcgc ccgggcgcga cgagctcgcc ggggccggct
     3181 gggacgacct cgtcgagcag gcgcgcagcg gcatggtcga gttcgcgggc ggcacgctgc
     3241 gcatttcgcc gaccccggca atgaccctga tcgacgtcga tggagggctg gctcccgccg
     3301 agctcgcggt cgccggagcg gccgaggcgg cgcgggcggt gcgccggctg ggcatcggcg
     3361 gctcgatcgg gatcgacctg ccgaccctcg tcggcaaggc cctgcgcctg gccgcggcgg
     3421 cggcgatcga cgcgcatctg ccgcagccgt tcgagcgcac ggcggtcaac ggcttcggct
     3481 tcgtccaggt cgtccgcccg cgccggcgcc cgtcgctgct cgaaatctgg agcgacgggg
     3541 cggcggccga agcccgcacc ttgctgcgcc gggcggcgct gtccggatcg gggccgcggc
     3601 ggctggtcgc gcacccggcg gtggtcgccg tgctcgagcg ccgccccgac tggctcgacg
     3661 cgctcgcccg ccagctcggc ggcgcggtga gcttgcgcgc cgacgcggcg cgtcccatgt
     3721 ccggcggcga tgccgagccc gcctaaagcc aagccgtgcc cgctatgcgg caagccgccc
     3781 agccaagccc acgcgccctt ctgcagccgc ggctgcaagg accgcgacct gctcaaatgg
     3841 ctcggcgaag gctatcggat tccgggcccg ccggctgatc ccgcggaact ggacagcgag
     3901 gaacgcgacg gttagggacc gcctcgcttc agcgggttac agagcccgcc ggcaggccca
     3961 ggtagctcag ttggtagagc acgtgactga aaatcacggt gtcggtggtt cgattccgcc
     4021 cctgggcacc attcccccac ccgaggaccc atgatcgacc tctacaccgc cgcgactccc
     4081 aacgggtaca aggtgtcggt cgcgctcgag gaactggagc tgccctacga gctccactgg
     4141 ctcaagctcg gcgcgggcga gcagaagcag ccgtggttcc tggcgatcaa ccccaacggc
     4201 cggatcccgg cgatcgtcga ccgcgacgcc ggcgacttcg cggtgttcga gagcggcgcg
     4261 atcctcatct acctggccga gaagaccggc cggctgatgc cgaccgacat gaagggccgc
     4321 agcctggtca tccagtggct gatgttccag atgggcgggc tcgggccgat gatgggccag
     4381 gccaatgttt tccaccgcta tctgagcgag cggatcccgg tcgcgatcga ccgctaccag
     4441 cgcgagagcc gccggctcct ggaagtgctc gacggccgcc tcgcggatca cgagtatctt
     4501 gccggggatt attccatcgc cgacatcgcc aactggtgct gggcgcggac ccacgactgg
     4561 tcgggactcg acgtgaccgg gctcgataac ctccagcgct ggttggccgc gatcgccgcc
     4621 cgaccggcgg tcgagaaagg cgccaacatc ccgccgcgca gcgacgagga gaaatcggtc
     4681 gcggacgcca ggagcatcct ggtctagggg ctcaggcctt ccacagctgc gggtaggcgc
     4741 gcctgagctt cgccagcttg ggccggtcgt ggaccagcac gtacgggtgg aacgggttgc
     4801 ggcggacgaa atcctggtgg ctcgcctcgg cgggatagaa ggcgccgctt tcgatccggg
     4861 tcgcgatcgg cttcttgtag atgcctgacg cgcgcagctg ggcgaggaag cgctccgcaa
     4921 gctgccgctg cgccgcggat tgcgggaaga tcgccgagcg gtagctgggt ccgacgtcgg
     4981 gtccctggcg gttgagctgg gtcgggtcgt gggccaccgt cgcgtagatc cggagcagct
     5041 gcgcgtagct gacctgccgc gggtcgtagg tgatgcgcac gacctcggcg tggcgggtgc
     5101 gctcggagct gaccgcggcg taattcgcgt cgcgcgcgct gccgccggca tagcccgaga
     5161 cgacgtcgcg gacgcccttc acatgctcga acaccgcctc catgccccag aagcagccgc
     5221 cggcgagcac cgccctctcg aggcgttggg gcgcggcctg cgcggcgccc gcgagcaggc
     5281 acaggatggc tgcggcggcg atccgccgtt tcactcggcg gcctccacca gcggcgcttc
     5341 gtaggcttcg ctcatgcggt tccggtcgtc ggcgaaccag gcgatggtcg cttcgaggcc
     5401 gtgctcgagg tcgaccctcg gcgcccatcg caggagctcg gtcgcgcgcg agatgtcggg
     5461 cttgcggcgg cgcgggtcgt cggtcggcag cggcaggcgg acgatgctcg agccgctgcc
     5521 ggtcatcgcc agcaccctct cgatcagctc gcggacgctc agctcctcgg ggttgccgat
     5581 gttgaccggc aggtccgcgg cgcgctcgct gcccatcagc cggaccagcc cctcgacggt
     5641 gtcgtcgacg tagcagaagc tgcgggtctg cgacccgtcg ccgtacatgg tgatgtcccg
     5701 gtcctcgagc gcctggcaga cgacgttgga gaccacccgg ccgtcgtcgg cgcgcatccg
     5761 cggcccatag gtgttgaaca gccgggcgac ccgagcctcg cagcggccgg cgcggatgaa
     5821 atcgaacgtc agagtctcgg cggcgcgctt gccctcgtcg tagcaggcgc gggggccggt
     5881 gcagctgacc gagccgcggt attcctcggt ctgcggatgg acctcgggct cgccatagac
     5941 ctcgctggtc gaggccagaa gcaaccgagc gccggtctcc tcggcgaggc ggagcaggtt
     6001 gcggctgccg accacgctcg acagcaaggt gtgctcgggg tcggcctggt agtgcggcgg
     6061 cgaggcggcg caggcgagat tgtagatgcg ggtgaacgct atgcggccgc cgcgaagcgc
     6121 cagcggcagc gggtcgatga cgtcgtgctc gaccaaccgg aagcgcgggt ggcgctcgag
     6181 gtgctcgatg ttccgccgcc ggccggtgac gaaattgtcg acgcagacga cggtcgcgcc
     6241 gtcggcgacc agccggtcgc acaggtgcga gccgatgaac ccggcgccgc cggcaacgag
     6301 cgcaacctgg tcaccgttgc acttcacccg atactccttg cctgcgccgc ttcgagtcca
     6361 ccggctggag cggcttcttg aggatattgt tccaaaaccg caaccacggg cgtggccgga
     6421 aatccggaac cggggcgcga tgagcgcggt tgaggggccg tccgaaccag cggagcagcg
     6481 acatgccagc caagtccaag gcgcagcaac gggccgcggg cgccgccttg tcggccaagc
     6541 gcggcgacac tccgaaaagc aagctcaagg ggccggcccg gcaaatggcg agctcgatga
     6601 gcgaaacgga gctcgacaag atggcctcga ccaagcgcaa ggggaaaccg gagcgcgtcg
     6661 ccaagaagaa gtaggacgcc gtccgtctag cgccagccgc gctcccgggc cgcctgttcc
     6721 tgcgcggcgt cgaacggctc gccctgaatc gtcgaatcga tcagagcggc gaggttcggg
     6781 tcggcaccgg cggccatccg gtagtcgccg ccttggccct cgggcgccgc gaacaggccg
     6841 cgcacccgcc agtcgccgcg ctcgcggcag gcgagcccgc tcgatcgctg gtcgctgaag
     6901 cttcggcaga tcgccccggc ctggtcgcgg aaggtcagtc cgacgcgcac gtcgccggcg
     6961 gcgccggcgc tcgccagctg ccgctcgagc gcatggtcga gcgccgcggc cgcgaccatc
     7021 cggccgccgc ggatctcgac cggcgacgag ccgccattgc tgtcgagcgt ggtgccgagg
     7081 ccgatcccga gcgccagggt cgccgccatc gccggccatt gcggcaccgg cagccacgag
     7141 ccgcgtgcgc tcttcgcccg gcggaacgcg ccgaagtcga cgatcgccga gcgcggcggc
     7201 ctggccgcct cgatcagccg ctcgggaacc ggcgcggcgg cgacggtgtc gaatgcgccg
     7261 cgcacccggg cgccgagcgc gcgatgctgc tcggccatcc gggcgaggcg agcgtcggcc
     7321 gcgacctcgg cctcgacctc ggcggcctcg gaagaatcga gctcgccgtc cagccaggcg
     7381 aagaatttgg tctcgtcaat cgtcatcgtc actcacccac cagcgccagc agcgcgttgc
     7441 ggccgcgcac cagccggctc gacacggtgc cgatcggcag gtcgagcaat tccgcggcct
     7501 cgcgatagcc gaagccttcg atcaggatca gcgcgacgac ctcgcgctgc tcgtcgggca
     7561 gccgctccat cgcccccatc atggtgtgca ggtcgatcgc cgcctcgact ccgtcgcgcg
     7621 ggtcgtggcc caggcgctcg gcctcctcgg ccggcgcttc gacccggtcc tggcggttgc
     7681 gcgagcggac cgtgtcgatc cacaggttgc gcatgatctt gtagagccag ctgtcgagcc
     7741 gcgtccccgg ctgccactgc gccttggcgc gaagggcgcg ctcgatggtc atctgagtca
     7801 aatcgtcggc gtcggcggcg ctccggctga ggccgtgcgc aaagcgccgc agccgcggaa
     7861 gcagcgcgca aagctcgtcc tcgaacgatt gtgctaggct cattcgtcct catgcggatg
     7921 aaacgacgct tcatcaccgt ttcatcccca agaggcatag aaacgggttc aacgatgtcc
     7981 agctttgttc gccacgcgct gatcgcagcc gccgccgccg gcctcgtcgc cggcgctgcc
     8041 gccgcgcagc tgctgccgtc cgtgaacgtt tccctcgtcg gcccggtcgt cggcccggtc
     8101 gcggtgccgg gcgtaccggt cgccgggccg gtcctgcagc agatcctgca gcag
//
LOCUS       DASTZO010000170         3495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_458186,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000170 DASTZO010000000
VERSION     DASTZO010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458186"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..1059)
                     /gene="tilS"
                     /locus_tag="VFU20_04780"
     CDS             complement(163..1059)
                     /gene="tilS"
                     /locus_tag="VFU20_04780"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006954930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU5285811.1"
                     /translation="MAPGARVGVAVSGGPDSLALLLLAAAARPGLVEAASVDHGLRPG
                     SRAEAEMVAELCKRIGVPHSILTIDWPAPPDRNLQARAREARYELLGSWAREQGLTAI
                     ATAHHLDDQAETLLMRLARGAGIGGLGSVRARRPLAKGVQLVRPLLGWRKVELADLVA
                     EAGVKPVDDPSNRDPRHDRVRMREWLKRADWADPERLAASAAWLNEADEALDWALAPL
                     AKQRVTRDGAALVVDPSEIPRELQRRLLLAAFAELGAPRPRGPELARALDTLRKGGTT
                     TLAGLKLEGGKTWRLRAAPPRR"
     gene            complement(1165..2067)
                     /locus_tag="VFU20_04785"
     CDS             complement(1165..2067)
                     /locus_tag="VFU20_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5285812.1"
                     /translation="MTANRLRLSIAAAALFAFGPAAAQAQRVITPEQRIERLESQLRQ
                     LQRQVFPRGQPADTAGFSDEPAATQSSVRTLNDRLNGLERQIADLVRQSEENGHRLTV
                     IQAELAKMRTDQDQRLGAIERAAAAAALPPAAGSATAPASAPPASVATVSPTPRPVSA
                     APGPAVASDAPDAGEIAYDEGYQLWRAGKYDEAISSLRAFSSAFPRHRRKSWADNLAG
                     RALLDKGEPRAAAEALLANYRSNPKGERAADSLYYLGQALMQLNQPGQACKAYAELES
                     VYGASMRAELKRLLPAAKAEARCS"
     gene            complement(2133..3011)
                     /locus_tag="VFU20_04790"
     CDS             complement(2133..3011)
                     /locus_tag="VFU20_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HEU5285813.1"
                     /translation="MAKKKGQGMVEPIDEPVEDLAEPEVASVGERLRLAREEKGLTLE
                     DVAAQTRIPRRHLESLENGDWERLPAPTYTTGFAKSYASAVGLDRTEIYEQLRVEMGG
                     VRPASQASEVFEPADPARTMPKWLVLGAILAVLVLVLAMSWLNERSFQGPDETVDEPA
                     ATAAAPAQPAPAPAPVPAAQGPVVLTANEQVWLQVKDGQTVLKEGLLEPGQSFEVPAS
                     ATAPVLMTGKPEALRISVGTADAPPIGPAATTVRNVSLLGPDLMRGPTSATPAASRAA
                     SPAPSAAPPAAQNSAG"
     gene            complement(3069..>3495)
                     /locus_tag="VFU20_04795"
     CDS             complement(3069..>3495)
                     /locus_tag="VFU20_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HEU5285814.1"
                     /translation="AEMLDQLLPRVDFLSIGTNDLTQFLFAADRADPRLAERYDWLSP
                     AILRFLKRVLDAARAAGVPVRLCGEMGGRPLETMALIGIGAENFSITPAAVGPVKAMV
                     RSLDAAAVRQRLEQLLARPPRNLRKALTDWARKRSVAVS"
BASE COUNT          462 a         1264 c         1237 g          532 t
ORIGIN      
        1 acagcacgcc gacccagatg agcaggctcc tggtccaggg attgccgggc ttcttctcgt
       61 tcatcgtccc tctcgcgtcg gcccaagcgg ccgtctgttc ctaaaatagt ctttcgagcc
      121 ctaataacaa tgcaatgctt aggtcccggt gaacggtggg gctcagcgcc gcggcggagc
      181 ggcgcgaagg cgccacgtct taccgccctc gagcttcaat cccgcaagcg tcgtggtccc
      241 gcccttgcgg agcgtgtcga gcgcccgggc gagctcgggg ccgcggggcc tcggagcgcc
      301 cagctcggcg aaggcggcga ggagcagccg ccgctgaagc tcccggggaa tttcggacgg
      361 gtcgacgacc aaggccgccc cgtcgcgggt gacgcgctgt tttgcgagcg gggccagcgc
      421 ccagtcgagc gcctcgtcgg cctcgttgag ccaggcggcg ctggcggcaa gccgttccgg
      481 gtccgcccaa tcggcgcgct tcagccattc gcgcatccgc acccggtcgt ggcgcgggtc
      541 gcggttggac gggtcgtcga ccggcttgac cccggcctct gccaccaggt ccgcgagctc
      601 gaccttgcgc cagccgagca gcggccggac gagctggacg cccttggcga gcggccgccg
      661 ggcgcgcacc gagccgagcc cgccgatccc ggcgccgcgg gcgaggcgca tcagcagcgt
      721 ctcggcctgg tcgtcgagat gatgcgcggt cgcgatcgcc gtcaggccct gctcgcgggc
      781 ccatgatccc agcagttcgt agcgggcctc ccgcgcccgg gcctgcaggt tacggtccgg
      841 gggcgccggc caatcgatgg tcaggatcga gtgagggacg ccgatgcgct tgcaaagctc
      901 ggccaccatc tcggcctcgg cgcggctccc cggccgcagc ccgtgatcga cgctcgccgc
      961 ttcgaccagg ccggggcgtg cggcggcggc cagcaacagc aaggccaggc tgtcgggccc
     1021 cccggacacc gcgactccga cccgggcgcc cggcgccacc agcgcgtcga ggtcggccgc
     1081 aaagcgcgtg acaagctgcg ggtcggggct gggaatacaa tgtgctcctg cgaacgcacg
     1141 agcccataac cgcggccgcc cggtctagct acagcgcgct tcggccttcg ccgccggcag
     1201 caggcgcttc aattccgccc gcatcgatgc tccatagacg ctttcgagct cggcataggc
     1261 cttgcacgcc tgcccgggct ggttgagctg catcagcgcc tggccgagat aatagaggct
     1321 gtcggcggcg cgctcgccct tggggttgga gcggtagttg gcgagcagag cctcggccgc
     1381 cgcgcggggc tcgcccttgt cgagcagcgc ccgcccggcg aggttgtcgg cccagctctt
     1441 gcggcggtgc ctggggaacg ccgacgagaa agcgcgcagc gagctgatcg cctcgtcata
     1501 cttgcccgcc cgccacaatt ggtagccttc gtcataggca atttcgccgg catcgggcgc
     1561 gtcgctcgcg accgccggtc ccggcgccgc gctgaccggc cgaggcgtcg ggctgaccgt
     1621 ggcgacgctc gcgggcggag cgctggctgg ggccgtggcg ctccccgccg ccgggggcag
     1681 ggcggccgcc gccgcggcgc gctcgatcgc tccgagccgc tggtcctggt cggttcgcat
     1741 tttcgccagc tcggcctgga tgacggtcag ccggtggccg ttctcctcgg attggcggac
     1801 caggtcggcg atctgccgct cgaggccgtt cagccggtcg ttcagagtcc gaaccgacga
     1861 ttgcgtggcc gctggctcgt cggagaagcc ggccgtatcg gcgggctggc ccctggggaa
     1921 aacctggcgc tgcaactgcc gcagctggct ttcaagacgc tcgatacgct gctcgggcgt
     1981 gatgacgcgc tgcgcctgcg cggcggccgg accgaacgcg aacagcgcgg cggcggcgat
     2041 ggataagcga agccgattgg ctgtcatgat tcccccagac ctggtgatgt gcgcgagcct
     2101 gcccaaccga tgctttcgcg gcgatgaatc cgtcagccgg cgctgttctg tgcggccggc
     2161 ggggcggcgg atggagcggg acttgcggcc ctcgacgcag ccggcgttgc cgacgtcggg
     2221 ccgcgcatca ggtcgggacc gagcaggctg acgttgcgaa cggtcgtcgc cgccggcccg
     2281 atcggcggtg catcggcggt gccgaccgaa atccgcagcg cctcgggctt gccggtcata
     2341 agcaccggcg cggtcgcgct cgccggcact tcgaagctct gccccggctc gagcagccct
     2401 tccttcagga ccgtctgccc atccttgacc tggagccaca cctgctcgtt ggcggtcagc
     2461 accaccggcc cctgcgcggc cggtaccgga gcgggcgccg gagcgggctg ggcgggcgcc
     2521 gccgcggtcg cggcgggctc gtcgacggtc tcgtccgggc cttggaacga tcgttcgttg
     2581 agccagctca tcgccagcac cagcaccagc accgccagga tcgctccgag caccaaccat
     2641 ttgggcatcg tccgcgccgg gtcggcgggc tcgaacacct ccgacgcctg cgaagccggg
     2701 cggactccgc ccatctcgac ccgcagctgc tcgtagatct cggtccggtc gagcccgacc
     2761 gccgacgcat agctcttggc gaagccggtc gtgtaggtcg gcgccggcag ccgctcccag
     2821 tcgccgttct cgaggctttc gaggtggcgc cgcggaatcc gcgtctgggc ggcgacgtcc
     2881 tcgagcgtca gccccttctc ctcgcgcgcg aggcgcagcc gctcgccgac cgatgcgact
     2941 tccggctcgg ccagatcctc caccggctcg tcgatcggct cgaccatccc ctgccctttt
     3001 ttctttgcca tcgccaatcc actcttcgaa gcgtgcggtc ggagtgtcaa ccgctcgcgc
     3061 gcggcttttc agctgaccgc caccgagcgc ttccgggccc agtcggtcag cgccttgcgc
     3121 aggttgcgcg gcggccgggc gagcagctgc tcgagccgct gccggaccgc cgccgcgtcg
     3181 agtgagcgga ccatcgcctt gaccgggccg acggcggcgg gcgtgatcga gaaattctcc
     3241 gcgccgatgc cgatcagggc catcgtctcc aatggccggc cgcccatctc gccgcacagc
     3301 cggaccggca ctccggctgc ccgggcggcg tcgagcacgc gcttcaagaa ccgcaggatc
     3361 gccgggctca gccagtcgta gcgctcggcg agccgcgggt cggcgcggtc ggcggcgaac
     3421 aggaattggg tcagatcgtt ggtcccgatc gacaggaaat cgacccgcgg aagcagctgg
     3481 tcgagcatct cggcg
//
LOCUS       DASTZO010000171         3605 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_193813,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000171 DASTZO010000000
VERSION     DASTZO010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3605)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3605)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3605
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193813"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..559)
                     /locus_tag="VFU20_04800"
     CDS             complement(<1..559)
                     /locus_tag="VFU20_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285815.1"
                     /translation="MTGAAARRAGIRARAAALLGAGLIAAAGLAYVADRKQAPASAER
                     AAKPELMLLTSLPLVFGEQFGLEGGGSPALKALETRYTVVPVGVADAATLRQGRLLLM
                     AHPLAQPAEALVDLDRWVRRGGRLLLLADPALDWHSERPLGDVLRPPPAFADTGLLAH
                     WGLRLDSPDERGPGLRRLGGHEVLTA"
     gene            complement(556..792)
                     /locus_tag="VFU20_04805"
     CDS             complement(556..792)
                     /locus_tag="VFU20_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285816.1"
                     /translation="MVDNAGTSAQRIRIGLTGLAFAFLLVLLGSVISRSGSDDRPANA
                     VEQAAANTPNEPLAELGVAPGGADSQANNSSDSR"
     gene            878..1246
                     /locus_tag="VFU20_04810"
     CDS             878..1246
                     /locus_tag="VFU20_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285817.1"
                     /translation="MTRTPLAIALAAAAALAGCNQQEVTLNTGLEDNVATNDVVLPPS
                     IAASKIYRCKDNSVVYIDWLSDNRSANVRTDRNAPPTHLVAPEAGQPMVAEGYSLTGA
                     AADSSVTLTRPGKGSQSCKA"
     gene            complement(1281..1754)
                     /locus_tag="VFU20_04815"
     CDS             complement(1281..1754)
                     /locus_tag="VFU20_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5285818.1"
                     /translation="MVRPIPEGYHSVTPYLIVDDAKAAIDFYGRAFGAEEKFRLPMGD
                     RIGHAELQIGDSVVMLADEFPDMGHLGPKSRGGPTCSLMHYVPDVDFAFRKAIDAGAT
                     EKRPVEDQFWGDRMGTLSDPFGHQWSLATHVREVPPEEMKQRMDEFSKQKQSEPA"
     gene            1869..>3605
                     /gene="purL"
                     /locus_tag="VFU20_04820"
     CDS             1869..>3605
                     /gene="purL"
                     /locus_tag="VFU20_04820"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEU5285819.1"
                     /translation="MSSPITPQIVAEHGLSEPEYARILKALGREPNLVELGIFSVMWS
                     EHCSYKSSRVHLKKLPTQAPWVICGPGENAGVIDIGDGDAAIFKMESHNHPSYIEPYQ
                     GAATGVGGILRDVFTMGARPIANLNALRFGRPDHPKTRHLVAGVVQGIGGYGNCVGVP
                     TVGGEVDFDSAYDGNILVNAMTVGVARADKIFYSAAAGIGNPVVYVGSKTGRDGIHGA
                     TMASADFSEDSEEKRPTVQVGDPFTEKLLIEACIQLMASDAIVAIQDMGAAGLTSSSV
                     EMASKGGVGIRLDMNKVPCREEGMTPYEMMLSESQERMLMVLKPGREAEDEAIFTKWE
                     LDFAVIGEVTDTGRMELVWNGETVADVPLGPLADEAPLYERPYAIPKPPEPLSDIPES
                     SDIAADLLKLMASPNLASRRWIWEQYDQSVGADTVQRPGGDAAVVRVHGTRKALAMTT
                     DCTPRYCYADPVEGGKQAVAEAYRNLCAVGARPLAITNCLNFGNPQRPEIMGQLVGCI
                     EGMAEACLALDFPVISGNVSLYNETNGKGILPTPTIGGVGVLDDVARHATIALKREGD
                     VLVLIGDSEGWLG"
BASE COUNT          590 a         1293 c         1196 g          526 t
ORIGIN      
        1 aggcggtgag aacctcatgg ccgcccagcc tgcgcagccc cggcccgcgc tcgtcgggcg
       61 aatcgagccg cagcccccaa tgcgcgagca gcccggtgtc ggcgaacgcc ggcggcgggc
      121 gcagaacgtc gcccagcggc cgctcgctgt gccagtcgag cgccggatcg gcgagcagca
      181 gcagccggcc gccccggcgc acccagcggt ccagatcgac cagcgcctcg gccggctggg
      241 cgagagggtg cgccatcagc aacagcctac cctgcctcag ggtcgcggcg tcggcgactc
      301 cgaccggaac caccgtgtag cgggtctcga gcgctttcag cgccggcgag cccccgccct
      361 cgagcccgaa ctgctcgccg aacaccagcg gcaggctggt caggagcatc agctccggct
      421 tggcggcgcg ctccgccgaa gcaggcgctt gcttgcgatc ggcgacatag gccaagcccg
      481 ccgccgcgat cagcccagcg ccgagcaacg ccgcggcgcg ggcgcggatc cccgcgcgcc
      541 gcgcagccgc gccggtcacc ggctgtcgct gctgttgttc gcctggctgt cggcgccgcc
      601 cggcgcgacc ccgagctcgg ccagcggctc gttgggcgtg ttcgcggccg cctgctcgac
      661 cgcgttcgcg gggcggtcgt cgcttcccga gcggctgatc accgagccca ggaggacgag
      721 caggaaagcg aaggcgaggc cggtcagccc gatccggatt cgctgcgcgc tggtgccggc
      781 attgtcgacc attgccgggc gatagctatg gcccgcatcg agacaatgca agccgcccat
      841 cttgacgcgg gcccgcgccg acgcgcaatg cgcgcaaatg actcggaccc cactcgccat
      901 cgccctggcc gccgccgccg cgctagccgg ctgcaaccag caggaagtta ccctcaatac
      961 cggactcgaa gacaatgtcg cgaccaacga cgtcgtcctt ccaccgtcga tcgcggccag
     1021 caagatctac cgctgcaagg acaacagcgt cgtctacatt gactggctgt cggacaacag
     1081 atcggccaac gtgcggaccg accggaacgc cccgccgacg catctggtcg cgccagaagc
     1141 cggccagcct atggtcgcgg aaggctattc gctgaccggc gccgccgccg attccagcgt
     1201 caccctgacc cggccgggca agggcagcca aagctgtaaa gcctgaagcc ccgccccacg
     1261 gtttcgtggg gcggaccgga ttacgccggc tcgctctgct tctgcttcga gaactcgtcc
     1321 atccgctgct tcatctcctc gggcgggact tcgcggacgt gggtcgcgag gctccactgg
     1381 tggccgaacg ggtcgctcag ggtgcccatc cggtcgcccc agaactggtc ctcgaccggc
     1441 cgtttctcgg tcgcgccggc gtcgatcgcc ttgcggaacg cgaaatcgac gtcggggacg
     1501 tagtgcatca ggctgcaggt cggtccgccg cggctcttgg ggccgagatg ccccatgtcc
     1561 gggaactcgt cggcgagcat gacgacgctg tcgccgatct ggagctcggc atggccgatc
     1621 ctatcgccca tcgggagccg gaacttctcc tcggcgccga acgcgcggcc gtagaagtcg
     1681 atcgccgcct tggcatcgtc gacgatcaga tagggcgtga cgctgtgata gccttcggga
     1741 atgggcctca ccatcgctgg tctcctcgat tccccccggg cggccttgtt actccttggg
     1801 cgcgagtcgc tccaaaccgg ctggccgcgc cctagccgcg ccccgcctcc cccgttaagg
     1861 ggcgcgcgat gagttccccg atcaccccgc aaatcgtcgc cgagcacggg ctttccgagc
     1921 ccgaatatgc gcgcattttg aaagcgctcg gccgcgagcc gaacctggtc gagctcggca
     1981 tcttctcggt gatgtggtcc gagcattgca gctacaaatc ctcgcgagtc cacctcaaga
     2041 aattgcccac gcaagcgccg tgggtgatct gcgggccggg cgagaacgcg ggcgtgatcg
     2101 acatcggcga cggcgacgcg gcgatcttca agatggagag ccacaaccac ccctcgtaca
     2161 tcgagcccta ccagggtgcc gcgaccggcg tcggcggcat attgcgcgac gtgttcacca
     2221 tgggcgctcg accgatcgcc aatttgaacg cgctgcgctt cggccgcccc gaccacccga
     2281 agacccggca cctggtcgcc ggagtcgtcc agggcatcgg cggctacggc aattgcgttg
     2341 gcgtgccgac ggtcggcggc gaggtcgatt tcgactccgc atacgacggc aacatcctgg
     2401 tcaatgcgat gaccgtcgga gtcgcgaggg ccgacaagat tttctattcc gccgccgcgg
     2461 gcatcggcaa tccggtggtc tacgtcggct ccaagaccgg gcgcgacggg atccacggcg
     2521 cgaccatggc ctcggccgat ttctccgagg acagcgagga aaagcgcccg accgtccagg
     2581 tcggcgaccc gttcaccgag aaattgctga tcgaggcctg catccagctg atggccagtg
     2641 acgcgatcgt cgccatccag gacatgggcg cggccggcct cacctcgtcg agcgtcgaga
     2701 tggcgagcaa gggcggggtc gggatccgcc tcgacatgaa caaggtgccg tgccgcgagg
     2761 agggcatgac cccctacgag atgatgctgt cggagtcgca ggagcggatg ctgatggtgc
     2821 tgaagcccgg ccgcgaggcc gaggacgagg cgatcttcac caagtgggag ctggacttcg
     2881 cggtgatcgg cgaagtcacc gacaccggcc gcatggagct tgtctggaac ggcgagaccg
     2941 tcgccgacgt gcccctcggc ccgctcgccg acgaggcgcc gctctacgag cgcccgtacg
     3001 ccatccccaa gccgcccgag ccgctgtccg acatccccga gagcagcgac atcgccgccg
     3061 atctgctcaa gctgatggcc tctcccaacc tcgccagccg ccgctggatc tgggagcaat
     3121 acgaccagtc ggtcggcgcg gacaccgtcc agcgccccgg cggcgacgcc gcggtggtcc
     3181 gggtccacgg cacccgcaag gcgctggcga tgaccaccga ctgcacgccc cgctactgct
     3241 atgcggaccc ggtcgagggc ggcaagcagg cggtcgccga ggcctaccgc aacctctgcg
     3301 cggtcggcgc aaggccgctg gcgatcacca actgcctcaa cttcggcaac ccgcaacggc
     3361 ccgagatcat gggccagttg gtcggctgca tcgaaggcat ggccgaggcc tgtctggccc
     3421 tcgatttccc cgtcatctcg ggcaacgtct cgctctacaa cgagaccaac ggcaagggca
     3481 tcctgccgac cccgaccatc ggcggcgtcg gcgtgctgga cgacgtggcg cgccacgcga
     3541 ccatcgccct gaagcgcgaa ggcgacgtgc tggtgctgat cggggacagc gagggctggc
     3601 tcggt
//
LOCUS       DASTZO010000172         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_91617,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000172 DASTZO010000000
VERSION     DASTZO010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91617"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="VFU20_04825"
     CDS             <1..497
                     /locus_tag="VFU20_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HEU5285820.1"
                     /translation="DAIALLKQDHRTVEDLFAQFEKASGEGRKQKLAGQICLELSVHA
                     AIEDQIFYPACEGKVDEDLLKEAYVEHDGAKVLIAEIEAGSAQGDDFFDAKVKVLQEQ
                     IEHHVEEEEKRMEGLFSQARKAGLDMDALGRQLAVRKAQLVETYRSTGVPKPELKTMS
                     EAQV"
     gene            514..2028
                     /locus_tag="VFU20_04830"
     CDS             514..2028
                     /locus_tag="VFU20_04830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5285821.1"
                     /translation="MTTSSFWYEDSGLEKRPPLQSDAECDLVVVGSGIAGLSSAYEAA
                     RFGWRVIVIDRAETIGGVMTPRTTAHLATELDDYYAYLIKARGEDDARIYHESQVAAV
                     NRIEAICRDEKIDADFARLDGILVPAEPAHMGELEQEYDACRAIGVEVEWLDEAPVRL
                     PDGTRALRFPNQGRFHPLKYVRGLVGAIEGRGGKIHGGTVYVDHEEEDGWVTIETEAG
                     HRIRARAALFATNSPVNDKVAIHTKMVPDRTYVIAGPVPKGSVPDALVWDTLEAYHYV
                     RLQPLGEAEDLLIVGGEDHRSGTVNDMDQRFAHLEAWTRERFPSFKDVRHRWSGQVME
                     PVDFMPYSGRNPGNRIIYVHTGDSGQGITNGVAGAINFAALLFGQKAHFAQVFDPGRK
                     PVAGTSLGEFLSGRAEDVANLSEYLTAGDIASIEDLKPGEGAVIRRGASKIAAFRKDD
                     GTVVERSAVCTHVGCIVHWNGLEQCWDCPCHGSQFQPDGTVINGPAIRPLAEAG"
     gene            2067..2960
                     /locus_tag="VFU20_04835"
     CDS             2067..2960
                     /locus_tag="VFU20_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5285822.1"
                     /translation="MAAGPHISAFFDECTNSVSYLVADPATRQAAVIDPVLDFDPASG
                     EMATRSAERILAAAADLGHSIALVLETHPHADHLTAAPFFRERTGARVAIGSGVREVQ
                     RIFAPVFGADDVKRDGGDFDLLLEDGDTLPLGELTIEVIATPGHTPACVSYKIGDAVF
                     VGDTLFMPDYGTARADFPGGDARRLYRSIHKLLSLPPDTRLFLCHDYKAPGRGEYAWE
                     TTVAEELASNVHVGAGVTEDAYVAMREARDATLCAPRLLLPAIQVNIRAGRLPPAEAN
                     GVRYLKIPVKLNGLPANQIFN"
     gene            3021..3275
                     /locus_tag="VFU20_04840"
     CDS             3021..3275
                     /locus_tag="VFU20_04840"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5285823.1"
                     /translation="MPRRHRIEVAHDATLVLSDGSEQSVTITDVSTGGFRLQTAETVP
                     IGAHVFLRVPRYGDFPAQIRWALGRDAGGVFLEPVELPNS"
     gene            3317..>4007
                     /locus_tag="VFU20_04845"
     CDS             3317..>4007
                     /locus_tag="VFU20_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010160749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5285824.1"
                     /translation="MNEHSIRDLVVVGGGTAGWMAAAALSRFLGKAGTRVTVIESDEI
                     GTVGVGEATIPPLIAYNNMLGINEDEFLAATQGTFKLGIEFVDWGALGSRYFHPFGMH
                     GQDVRGVPFHQLYLRESKRRALPDIAEWSMSAVAAALGRYARPGPDARLPLSQLFYAF
                     HFDAGLYALFLRDYAERGGVRRIEGRIVDTALDGETGHVESVTLADGTAVAGELFVDC
                     SGFRGLLIEQAL"
BASE COUNT          668 a         1345 c         1372 g          622 t
ORIGIN      
        1 tggatgcgat cgcgctgctc aagcaggacc accgcaccgt cgaagacctg ttcgcgcagt
       61 tcgagaaagc cagcggcgag ggccgcaagc agaagctcgc cgggcagatc tgcctcgagc
      121 tgtcggtcca cgccgccatc gaggaccaga ttttctatcc ggcgtgcgag ggcaaggtcg
      181 acgaggacct cttgaaggaa gcctatgtcg agcatgacgg cgccaaggtg ctgatcgccg
      241 agatcgaggc cggaagcgcc cagggcgacg acttcttcga cgccaaggtg aaggtgctcc
      301 aggagcagat cgagcaccat gtcgaggaag aggaaaagag gatggagggc ctgttcagcc
      361 aggcgcgcaa ggccggtctg gacatggacg cgctcgggcg tcagctcgcc gtgcgcaagg
      421 cgcagctggt cgaaacctac cggtcgaccg gcgttcccaa gcccgaattg aagacgatga
      481 gcgaggccca ggtctgaggt cggacggtcg gcgatgacca ccagcagctt ctggtacgag
      541 gattcggggc tggagaagcg cccgccgctg cagtcggacg ccgagtgcga tctggtcgtc
      601 gtcggttcgg gcatcgccgg cctgtcgagc gcctacgagg ccgcgcgctt cggctggcgg
      661 gtgatcgtca tcgaccgcgc cgagaccatc ggcggggtga tgactccgcg caccaccgcc
      721 catctcgcga ccgagctcga cgattattat gcctatttga tcaaggcccg cggcgaggac
      781 gacgcgcgca tctatcacga aagccaggtc gcggcggtca accgcatcga ggcgatctgc
      841 cgcgacgaga agatcgacgc cgatttcgcg cggctcgacg ggatcctggt gccggccgag
      901 cccgcgcaca tgggcgagct cgagcaggaa tatgatgcgt gccgggcgat cggagtcgag
      961 gtcgagtggc tcgacgaggc gccggtcagg ctgccggacg ggactcgggc gctgcgcttc
     1021 cccaaccagg gccgcttcca cccgctcaaa tatgtccgcg ggctggtcgg agcgatcgag
     1081 ggccgcggcg gaaaaatcca cggcggcacc gtctatgtcg accatgaaga ggaggacggc
     1141 tgggtcacga tcgagaccga ggccggccac cggatccgcg ccagagcggc gctgttcgcg
     1201 accaactcgc cggtcaacga caaggtggcc atccacacca agatggtccc ggaccgcacc
     1261 tatgtgatcg ccgggccggt gcccaaaggc tcggtgccgg acgcgctcgt ctgggacacg
     1321 ctcgaggcct atcattacgt tcgcctgcag ccgctgggcg aggccgagga tttgctgatc
     1381 gtcggcggcg aggaccaccg cagcggaacc gtcaacgaca tggaccagcg gttcgcgcat
     1441 ctcgaggcct ggacccgcga gcgctttccg tccttcaagg acgtccgcca ccgctggtcg
     1501 gggcaggtga tggagccggt cgatttcatg ccttactcgg gccgcaaccc gggcaaccgc
     1561 atcatctacg tccacaccgg cgacagcggc cagggcatca ccaacggggt cgccggggcg
     1621 atcaatttcg ccgctttgct gttcgggcag aaagcgcatt tcgcccaagt gttcgatccc
     1681 ggccgaaagc cggtcgccgg caccagcctc ggcgagttcc tttccggccg ggccgaggat
     1741 gtcgccaacc tgtccgaata tctgaccgcc ggcgacatcg cctcgatcga ggacttgaag
     1801 ccgggcgagg gcgcggtcat ccgccgcggc gcatcgaaga tcgccgcgtt ccggaaggat
     1861 gacgggacgg tcgtcgaacg ctcggccgtc tgcacccacg tcggctgcat cgtccactgg
     1921 aacgggctcg agcaatgctg ggactgcccg tgccacggct cgcagttcca gcccgacggg
     1981 acggtgatca acgggccggc gatccggccg cttgccgagg cgggctgatc gagtcatcct
     2041 gcccgcctgc gctggggagc tgcaccatgg cggcagggcc gcacatctcg gcatttttcg
     2101 acgagtgcac caacagcgtc tcctacctgg tcgcggaccc ggcgacccgg caggcggcgg
     2161 taatcgaccc ggtgctcgat ttcgacccgg cgagcgggga aatggcgacc cgctccgccg
     2221 agcgcatcct cgccgccgcg gccgatctgg ggcactcgat cgcgctggtg ctcgaaaccc
     2281 acccccacgc cgaccatctg accgcggcgc cgttcttcag ggagcggacc ggcgccaggg
     2341 tcgcgatcgg ctcgggggtc cgcgaggtgc agaggatttt cgcgccggtg ttcggcgccg
     2401 acgacgtcaa gcgcgacggc ggcgacttcg acctgctgct cgaggacggc gacaccctgc
     2461 cgctcggcga gctgaccatc gaggtcatcg ccacgcccgg ccacacgccc gcctgcgtca
     2521 gctacaagat cggcgacgcg gtgttcgtcg gcgacacatt gttcatgccc gactatggaa
     2581 ccgcccgggc cgacttcccg ggcggtgacg cgcggcggct ctaccgctcg atccacaaat
     2641 tgctgtcgct tccgcccgac acgcggctgt tcctgtgcca cgactacaag gcgccgggcc
     2701 gaggcgaata tgcgtgggag acgacggtcg ccgaagagct ggcgagcaac gtgcatgtcg
     2761 gcgcgggggt caccgaggac gcctatgtcg cgatgcgcga ggcgcgggac gcgacgctgt
     2821 gcgccccgag gctcctgctg cccgcgatcc aggtcaacat ccgcgccggc cgccttccgc
     2881 cggcggaggc gaacggagtc cgctatttga agattccggt caagttgaac ggcttgcccg
     2941 ctaaccaaat cttcaactga tctggattag gatcgcccgg ccgaatctga aagtggaacg
     3001 tggaccgaat tcccgggcaa gtgccgcgga gacaccgcat cgaggtcgcg catgacgcga
     3061 cccttgtgct gtccgacggc tccgagcagt cggtgaccat caccgacgtg tcgacgggcg
     3121 gcttccggtt gcagaccgcc gagaccgtcc cgatcggcgc acatgtgttc ctgcgcgtgc
     3181 cgcgctacgg cgacttcccg gcgcagatcc gctgggcgct cggccgcgat gccggcggcg
     3241 tgttcctcga gccggtcgag ctgcccaaca gctgagcgcg ttgcagattt agaggcgctg
     3301 ccccaagctt cgcccgatga acgagcattc gatccgcgac ctggtcgtcg tcgggggagg
     3361 caccgccggc tggatggccg cggccgcgct gtcgcgcttc ctcggcaagg ccggcacgcg
     3421 ggtcaccgtc atcgaatccg acgagatcgg caccgtcggg gtcggcgagg cgacgatccc
     3481 gccgctgatc gcctacaaca atatgctcgg gatcaacgag gacgagttcc tcgccgcgac
     3541 ccaggggacg ttcaagctcg gcatcgagtt cgtcgactgg ggcgcgctcg ggtcacgcta
     3601 tttccatccg ttcgggatgc acgggcagga cgtgcgcgga gtcccgttcc accagctcta
     3661 tttgcgcgaa agcaagcgcc gcgcgcttcc cgacatcgcc gaatggtcga tgagcgcggt
     3721 cgccgccgct ctcggccgct acgcccgccc cggccccgac gcccgcctgc cgctgtcgca
     3781 actgttctac gcgttccatt tcgacgccgg gctctacgcc ctgttcctgc gcgattatgc
     3841 cgagcgcggc ggggtgcggc ggatcgaggg caggatcgtc gacaccgcgc tcgacgggga
     3901 gaccggccat gtggagtcgg tcactctggc cgacggcacc gcggtcgcgg gcgagctgtt
     3961 cgtcgactgc tcgggctttc gcggcctgct gatcgagcag gcgctgg
//
LOCUS       DASTZO010000173         9858 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_324044,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000173 DASTZO010000000
VERSION     DASTZO010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 9858)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9858)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9858
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_324044"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2434)
                     /locus_tag="VFU20_04850"
     CDS             complement(<1..2434)
                     /locus_tag="VFU20_04850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5285825.1"
                     /translation="MKRIEIALRAGAALAALAIATSASAQSTLPPPDVAETPATTAQE
                     PTAGGDIIVTGSRIRRDPLSQEQPIVFVDQEDIAKTGLNSVNDVLQRLPSSGGGLNSK
                     INNSGNLGNPPDGGGVGAGAAEVDLRYLGSRRVLVLVDGIRYVNGASASGVPGSTDLN
                     TIPESAVERIEVLQDGASAIYGSDAIAGVVNIITKKRQEGFQASAQLGGYDEGDGFTQ
                     NYQLSWGNGSQGPLELVVGGNYVKQGSVSTDDRDLSRFPNPYAESCFEGGCSGFIPMG
                     RYQFNGFFGGVTQTLIATTNTPTLTNFRPFVDPADRFNFGPFNFLQIPLERYGAFANL
                     KYELADNMNFSAKGVWNRRDSKNKAAPLPFGIGPTSGLTPVLSGLGVHSTNPFNPFGV
                     DLVGDNPLTPAANDGNLRAIFRRYVEGGPRRFNQTVDTWYGVATLDGRFDMGGNNWFW
                     DVNAAYGRNKAEQRMLGNIHSGRLGIALGPLATCTATPGCVPFNLFGGAGSITTQMID
                     YVTFIQNDSSEQKVLDLTGNLSGSLFQLPGGPLGVAVGVEYRDLRGRFDPDEVVAAGF
                     SSDIPALPTKGSYNVKEAYAELNAPLLADRPFFELLELTGAVRFSEYSRGLGSTTTFK
                     AGLNWKPTQDLRLRGSWAEGFRAPQIGELFGSQSRFDQTLSDPCSSHATNTQPRRFSN
                     DATVRANCIAMGVPADGSYIQANPQISVVVGGNENLAPETSKSWVIGGVYSPRFLPGL
                     SAEVNWYKITVKDAIQTVNANTTLVNCVVENDPAACALTTRVSGELLEISGLLQNIAA
                     IKTNGIDLNLAYR"
     gene            complement(2570..3502)
                     /locus_tag="VFU20_04855"
     CDS             complement(2570..3502)
                     /locus_tag="VFU20_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5285826.1"
                     /translation="MTDRPRIAVLLPCFNEEATIAATVAGFRAALPAAEIYVYDNNSR
                     DRTREIAAAGGAVVRTERQQGKGHVVRRMFADIDADVYVMADGDLTYDPGSAQAMVDR
                     LLADQLDMVVGTRRHDEKGAYRGGHVLGNRLFTGILGRLFGRSFSDIFSGYRVFSRRF
                     VKSFPVLSSGFEIETEMSVHALELRMPVGEMETAYASRPEGSQSKLSTFGDGWRILKT
                     IATLYRTERPVLFFGSIGALLLLAALVLAVPLIVTYLDTGLVPRLPTAILITGMTIVA
                     VLCFFAGLILDTVTRGRREVRRLAYLSLAPPGSA"
     gene            complement(3587..5209)
                     /gene="tig"
                     /locus_tag="VFU20_04860"
     CDS             complement(3587..5209)
                     /gene="tig"
                     /locus_tag="VFU20_04860"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU5285827.1"
                     /translation="MKTVEMENQGLKRAFMLTIAAKEIEARVDQEVRRIAPQVRMPGF
                     RPGKVPPNLIRKMHGDSLQRDALSGAVQDGVQKLLEEKKLRPALQPEVELDERYEPGK
                     DAEVRVRLEALPDVPAPQIDGLELERLTVEVDEAAVDEQLQNLARSNKSWSDAPKKHA
                     AQLGDLVVIDYAGTVGGEAFEGGSGEDMSVELGSGRLIPGFEEGLVGAKAGDQRDVKV
                     KFPADYPSDKLKGKAASFAVTVKAVKLAGEARVDEEFAKSLGLNSLEELRKLIRDQQQ
                     QELNGLTRTHMKRRLLDQLAAKHDFPVPVSMVEAEFQNIMQQLRHEASHEEDPKAALA
                     EIEKDADEYRGIAERRVRLGLLLSEIGSANGIQVSEQEMNRLIAQAASQYQGQDRERF
                     VQYVQQEPMAAAQLRAPLYEDKVVDFLFSKAAVSERKASRAELEADLESEEGHVHGPG
                     CGHEHLAAAKPAAKGKKAAKPAKAEAKPAKAEAKPEKPAKGPVEEKSTARPKPAKPLK
                     DGAAGKPADKKPAKAAAKAPAKSAAKKAPAKK"
     gene            complement(5261..5345)
                     /locus_tag="VFU20_04865"
     tRNA            complement(5261..5345)
                     /locus_tag="VFU20_04865"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5309..5311),aa:Leu,seq:tag)
     gene            complement(5668..6300)
                     /locus_tag="VFU20_04870"
     CDS             complement(5668..6300)
                     /locus_tag="VFU20_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285828.1"
                     /translation="MTRTPKLILAAAALALSGCSSVGDGGGFGSDYYSLVRVRSVQVG
                     DGSLAVTPPRPWNKTRRVFFFDDIRQVEDWTLNGPLLDGISFVTGLRSGRYLIRQSRR
                     EDRQVPKFRSDMTAPEITAMLESLYRVRGGSIDFRTLSLQPRRFLGANGFQFDFEHLD
                     TDELWRRGRAVGAVIDGKLYLILFDAARSHYYGNAIGDFETIVASARLRR"
     gene            complement(6317..7543)
                     /locus_tag="VFU20_04875"
     CDS             complement(6317..7543)
                     /locus_tag="VFU20_04875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEU5285829.1"
                     /translation="MCFRCYELNRRSLLVGGGVAAASLTTGVAQARIRPADMRPLIGP
                     GYRPTDRDEIGLWQQMARVEEEVAGSNLLIKDPELIRYLQNLIGTVGGPAAKDMRIYL
                     ARIPDFNAVMFPSGFTVIFSGLLLRMRNEAQLAGVIAHESGHFLRRHMIRMWRDMRRK
                     TDLFAIGAMVAGVAGGATGVNTGDLTQLAQLGTILSLLRYNRVMEAESDAMGARLLAE
                     NGYPAIEMSNTWEQLIGELNASARHRRKRRERRIGLFETHPSPESRLADLKASAAEVT
                     VPGRVYDSRRGRYLQALGPIRKMLLDDQVKLNDPGASQYLIETLARDGWNGLLRFYEA
                     EVWRLRNRQGDDRRAAQGYAIAVAYPDAPPEAWRWHGLSLMKAGRHSEARAALSRYLS
                     MQPNAADAPFIRQMIG"
     gene            complement(7543..8319)
                     /locus_tag="VFU20_04880"
     CDS             complement(7543..8319)
                     /locus_tag="VFU20_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285830.1"
                     /translation="MRHLGIALALALSVPVAGCVQTRQFADLQFTPPQGDYRLLVMRP
                     DVTVNSVTTGGLPEPRADWTEQARANITAALRAQQAERGGKVQILGRRNELAGVSEET
                     VADLERLHSAVGNSIALHKYLGAYLPTKPRRGLDYTLGAQAVEVGRKTGFDYALFMVA
                     EDNIVSGGRVALQVLGIAGCFVGFCAPNIGGGGQFAYASLVDLKTGEVVWFNVVQTGS
                     QIAGIKMGDIRTPQGAAQLVDRLVGRMKPGRDVRRAMERR"
     gene            complement(8421..>9858)
                     /locus_tag="VFU20_04885"
     CDS             complement(8421..>9858)
                     /locus_tag="VFU20_04885"
                     /inference="COORDINATES: protein motif:HMM:NF013799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5285831.1"
                     /translation="FRNGYQGHEATETCPGHSTMLTGLRPARTGIIANYWTDQSLTRS
                     DKSVYCAEDPAVPGSSSTAYTVSPVHLKGVTLGERLKASSPASRNVAVGGKDRAAVMM
                     GGRQVDQRWYWDGKRFASDLRAAPPQSVARANAAVGALIAAPQPPLEPPELCSAKARA
                     VPVEGGGKPVGDGRFARAAGDGSAFRASPAFDGAVLALAAGLIQDMKLGQDAAPDLLS
                     VGLSATDYVGHTYGVRGQEMCLQLLSLDRDLGDFFKLLDRSGLDYAVALTADHGFEDI
                     PERLRQQEAKAAIRVDPALEATAVGKRIAAKLGLSGPVLIGDYFGDVYLDRALKPADR
                     SRALREAVAAYAAHPQVETAFTADQLRRIALPTTSPERWSLAERARASFDLERSGDLV
                     VLLRQHVTPVPDTSRYIATHGSPWDYDRRVPILFWRPAMASADRNEAVETVDILPTLA
                     AMLGLRVDSASIDGRCLAGIQGVACPPR"
BASE COUNT         1553 a         3300 c         3391 g         1614 t
ORIGIN      
        1 tgcgataggc caggttgaga tcgatgccgt tggtcttgat ggcggcaata ttctgcagca
       61 ggcccgaaat ctcgagcaat tcaccactga cacgggtcgt caaagcgcac gccgccggat
      121 cattctcgac cacgcagttc acgagcgtcg tattggcgtt gactgtctgg atcgcatcct
      181 tgaccgtgat cttgtaccaa ttcacctcgg cggaaaggcc cggcaggaaa cgcgggctgt
      241 agacgccgcc gatcacccag cttttcgacg tttcgggcgc gagattttca ttgccaccga
      301 cgacgaccga gatctgcggg ttggcctgga tgtagctgcc gtcggcggga acgcccatgg
      361 cgatacagtt ggcgcgaacc gtcgcatcgt tgctgaatcg gcgcggctgg gtgttggtcg
      421 cgtgcgacga acagggatcg ctgagcgtct ggtcgaagcg cgactggctg ccgaacagct
      481 cgccgatctg aggcgcacgg aacccttccg cccacgagcc gcggagccgc aaatcctggg
      541 tcggcttcca gttgaggccg gccttgaacg tcgtggtcga gccgagtccg cgggaatatt
      601 cggagaatcg caccgcgccg gtcagttcga gcagctcgaa gaacggccgg tcggcgagca
      661 gcggagcgtt gagctcggcg tacgcttcct tgacgttgta gctgcctttg gtcggcaaag
      721 cgggaatgtc cgagctgaag ccggccgcga ccacctcatc ggggtcgaac cggcccctca
      781 gatcgcgata ttcaacgccg acggcgactc ccagcggccc tcccggaagc tggaacaggc
      841 tgccggacag gttgcccgtc agatcgagaa ccttctgctc gctgctgtcg ttctggatga
      901 acgtcacata gtcgatcatc tgcgtggtga tcgaaccagc gccgccgaac aggttgaagg
      961 gaacgcatcc cggcgtggcc gtgcaggtgg cgagcggccc gagcgctatg cccaatcggc
     1021 ccgaatggat gttgccgagc attctctgct cggccttgtt gcggccatag gcggcattga
     1081 cgtcccagaa ccagttgttg cctcccatgt cgaaccggcc gtcgagcgtc gcgacgccgt
     1141 accaggtgtc gaccgtctga ttgaaccggc gcggaccacc ctcgacgtag cggcggaaga
     1201 tggctcgcaa gttgccgtcg ttcgctgccg gcgtgagcgg attgtcacca acgagatcga
     1261 ccccgaatgg attgaacggg ttggtggagt ggacgccgag cccgctaagc accggcgtaa
     1321 ggccggaggt cggaccgatg ccgaacggca aaggcgccgc cttgttcttc gaatcacgcc
     1381 ggttccagac gcccttggcc gagaaattca tgttgtcggc cagctcatat ttgagattgg
     1441 cgaaggcgcc gtagcgctcc agcgggatct ggaggaagtt gaacgggccg aaattgaagc
     1501 ggtcggccgg gtcgacgaaa ggccggaaat tggtcagagt cggcgtgttg gtcgtggcga
     1561 tcagggtttg agtaacgccg ccgaaaaagc cgttgaactg gtaccggccc atcgggatga
     1621 agcctgagca gccgccttca aagcagcttt cggcataagg gttcgggaaa cgggacaggt
     1681 cgcggtcatc ggtcgaaacc gatccctgct tcacataatt tccgccgacg acaagctcga
     1741 gcgggccctg gctgccattg ccccagctca gctgatagtt ttgggtgaag ccatcacctt
     1801 cgtcatagcc gccgagttgc gcgctggcct gaaaaccctc ttgccgcttc ttggtgatga
     1861 tgttcaccac gccggcaatg gcgtccgagc cgtagatcgc cgaggcgccg tcctggagca
     1921 cctcgatgcg ctcgaccgcg ctctcgggaa tggtattgag gtcggtcgaa ccgggaacgc
     1981 cggaggcgga agcgccattg acgtaacgga tgccgtcgac gagcacgagc actcggcgcg
     2041 agcccagata gcgcaggtcg acctcggctg cgccggcacc gacgccaccg ccgtccggcg
     2101 ggttgccaag gttgccgctg ttgttgatct tgctgttgag gccgccgccc gagctgggca
     2161 ggcgctgcaa cacgtcgttg accgagttga ggccggtctt ggcgatgtcc tcctggtcga
     2221 cgaaaacgat cggctgttcc tgggacagtg ggtcgcggcg gatacgcgaa ccggtgacga
     2281 tgatgtcgcc tccggcggtc ggctcctggg cggtcgtcgc cggagtctcg gccacgtccg
     2341 gcggcggaag agtgctttgc gctgatgccg aagttgcgat ggcgagcgcg gccagcgcgg
     2401 cgccggcgcg aagagcgatt tcgatccgct tcatgaattt tcccctgttc caccgcagcg
     2461 aaagcagccg cgaagacgcg aagggcaata aatcagacgc gggagtggag gaagggcctc
     2521 agtcaccagg acgacacaat ccgtcccggg tgatgcaaat cgtctacact caagcgctcc
     2581 cgggcggggc gagcgacaga taggcgagcc ggcgcacctc gcggcgtccg cgggtgacgg
     2641 tgtcgaggat caggccggcg aaaaagcaca gcaccgcgac gatcgtcatg ccggtgatca
     2701 ggatcgcggt cggcagccgc ggcaccaggc cggtgtcgag ataggtgacg atcagcggca
     2761 cggcgaggac cagcgcggcc agcagcagca gcgctccgat gcttccgaaa aacagcaccg
     2821 gccgctcggt ccggtagagc gtggcgatgg tcttgaggat ccgccagccg tcgccgaacg
     2881 tcgacagctt ggactgcgag ccttcggggc gcgaggcata agcggtctcc atctcgccca
     2941 ccggcatcct gagttcgagc gcgtggacgc tcatctcggt ctcgatctcg aaccccgacg
     3001 acaggaccgg gaagctcttg acgaagcgcc tcgagaacac ccggtagccc gagaaaatgt
     3061 ccgagaagct gcggccgaac agccggccga ggatgccggt gaacagccgg ttgccgagca
     3121 cgtggccgcc ccggtacgcg cccttctcgt cgtggcggcg ggtgccgacg accatgtcga
     3181 gctggtcggc gagcagcctg tcgaccatcg cctgggccga tcccgggtcg taggtcaggt
     3241 cgccgtcggc catcacgtag acgtcggcgt cgatgtcggc gaacatccgc cggacgacat
     3301 ggcccttgcc ctgctggcgc tcggtgcgga ccactgcccc gccggcggcg gcgatctcgc
     3361 gggtgcggtc gcggctgttg ttgtcgtaga cgtagatttc cgccgccggc agcgcggccc
     3421 ggaagcccgc gaccgtcgcg gcgatggtcg cttcctcgtt gaagcacggc aacagcacgg
     3481 cgatgcgcgg tcggtcggtc attggccccc catcgtcatc cccgcgaaaa cggggaccca
     3541 gcgtcttgcg ctgttactgg gttcccgctt gcgcgggaat gacgattcat ttcttcgccg
     3601 gagctttctt cgcagcgctc ttggccggcg ccttggcggc agccttggca ggcttcttgt
     3661 cggcgggctt gccggcggcg ccgtccttga gaggcttggc gggtttcggc ctggcggtgg
     3721 atttctcctc gaccggtccc ttggccggct tctctggctt ggcctcggcc ttggccggct
     3781 tggcctcggc cttggccggc ttggccgctt tcttgccctt cgccgccggc ttggccgccg
     3841 cgaggtgctc gtggccgcag ccggggccgt ggacatggcc ttcctcgctc tcgaggtcgg
     3901 cctcgagctc ggcgcggctc gccttgcgct cgctgaccgc cgccttcgag aacaggaagt
     3961 cgaccacctt gtcctcgtac agcggggcac gcagctgggc cgccgccatc ggctcctgct
     4021 ggacgtactg gacgaagcgc tcgcggtcct ggccctgata ttgcgacgcg gcctgggcga
     4081 tcagccggtt catctcctgc tcgctgacct ggatcccgtt ggcgctgccg atctccgaca
     4141 gcagcagccc gaggcggacc cggcgctcgg cgatgccgcg atattcgtcg gcatccttct
     4201 cgatctcggc cagggcggcc ttcggatcct cctcgtggct cgcctcgtgg cgaagctgct
     4261 gcatgatgtt ctggaactcg gcctcgacca tcgacaccgg caccgggaaa tcatgcttgg
     4321 cggccagctg gtcgagcagc cggcgcttca tgtgggtgcg ggtcagcccg ttgagctcct
     4381 gctgctgctg gtcgcggatc agcttgcgca gttcctcgag gctgttgagc ccgagcgact
     4441 tggcgaactc ctcgtcgacc ctggcctcgc cggcaagctt caccgccttg acggtcaccg
     4501 cgaagctcgc cgccttgccc ttcagcttgt cggacggata gtcggccggg aacttgacct
     4561 tgacgtcgcg ctggtcgccg gccttggcgc cgaccaggcc ctcctcgaag cccgggatca
     4621 gccggccgga gccgagctcg accgacatgt cctcgcccga gccgccctcg aacgcctcgc
     4681 cgccgaccgt gccggcatag tcgatcacca ccaggtcgcc gagctgcgcg gcatgcttct
     4741 tgggcgcatc gctccagctc ttgttggatc gggcgaggtt ctgcagctgc tcgtcgaccg
     4801 cggcctcgtc gacctcgacc gtcagccgct cgagctcgag cccgtcgatc tgcggcgcgg
     4861 gcacgtcggg aagcgcttcc aggcggaccc ggacctcggc gtccttgccg ggctcatagc
     4921 gctcgtcgag ctcgacctcg ggctgcagcg ccgggcgcag cttcttctcc tcgaggagct
     4981 tctggacgcc gtcctggacg gccccgctca aggcgtcgcg ctggagcgag tcgccgtgca
     5041 tcttgcggat caggttcggc ggcaccttgc cgggacggaa gccgggcatg cgcacctgcg
     5101 gagcgatccg ccgcacttcc tggtcgacgc gcgcctcgat ttccttggcg gcgatcgtca
     5161 gcatgaaggc ccgcttgagg ccctggtttt ccatctcgac ggttttcatg gcttcgtgtg
     5221 caatcccaaa actagcgtgt cgaagcggcg gcgggcggcc tggtgcgcgc ggagggactc
     5281 gaacccccac atctttcgac aacaggacct aaacctgccg cgtctaccaa ttccgccacg
     5341 cgcgcgcggt tcgcttcgaa ggtgcgcggc ctatagcaag gatggcttgc ggggcaagcg
     5401 cggcgggcgg cgggcaaccg gcggcccagc ccgggcgttc ggatagcaag gagacgcgaa
     5461 gccatgcagc aactgccgcc attttccgag gagcccgtcc agccaccgca gccgggccag
     5521 cccccgcaac ccggccagcc atcgcccccg ggccgcccga gcgaagcccc gaacgagacc
     5581 ccgccgactg ctccggacat cgacgttccg tcgccgtcgc agccggggac cgacccctcg
     5641 ccgacgccga tctcgccggt cggctgatta gcgccgcagc cgcgccgagg cgacgatcgt
     5701 ctcgaagtcg ccgatcgcat tgccgtaata atgcgagcgc gcggcgtcga acaggatcag
     5761 gtagagcttg ccgtcgatca ccgccccgac cgctcggccg cggcgccaca gctcgtcggt
     5821 gtcgagatgc tcgaaatcga actggaagcc gttggccccg aggaaccggc gcggctggag
     5881 cgacagggtc cggaagtcga tcgagccgcc gcgcacccgg tacaggctct cgagcatcgc
     5941 ggtgatctcg ggcgccgtca tgtccgagcg gaacttgggc acctggcggt cctcgcgccg
     6001 gctctggcgg atgaggtagc ggccgctgcg caggccggtg acgaagctga tcccgtcgag
     6061 cagcggcccg ttgagggtcc agtcctcgac ctggcggata tcgtcgaaga agaacacccg
     6121 ccgggtcttg ttccacggcc gcggcggagt gaccgccagc gagccgtcgc cgacctggac
     6181 gctgcgcacc cggaccagcg agtaataatc gctcccgaac ccgccgccgt cgccgaccga
     6241 cgagcagccg gacagggcga gcgcggctgc ggcgagaatc agcttcggcg tgcgcgtcat
     6301 cggcttcaag ctccggttag ccaatcatct ggcggatgaa gggtgcgtca gcggcattgg
     6361 gctgcatcga aaggtagcgc gacaaggccg cgcgggcttc cgaatggcgg ccggccttca
     6421 tcagcgacag gccgtgccag cgccacgcct cgggcggcgc atcgggataa gcgaccgcga
     6481 tcgcatagcc ctgggcggcc cggcggtcgt cgccttggcg gttgcgcagg cgccagacct
     6541 cggcctcgta gaagcgcagc aggccgttcc agccgtcgcg cgccaatgtc tcgatcagat
     6601 actggctggc gcccgggtcg ttcaacttga cctggtcgtc gagcagcatc ttgcggatcg
     6661 gcccgagcgc ctggagatag cggccgcggc ggctgtcgta gacgcggccg ggcacggtca
     6721 cctcggccgc cgacgccttg aggtcggcga ggcgcgattc gggcgacggg tgggtctcga
     6781 acaggccgat ccggcgctcg cggcgcttgc gccgatgccg ggcgctggca ttgagctcgc
     6841 cgatcaattg ctcccaggtg ttcgacatct cgatcgccgg atagccgttc tcggcgagca
     6901 gccgggcgcc catcgcgtcg gattccgcct ccattacccg gttgtagcgg agcagcgaca
     6961 ggatcgtgcc gagctgggcg agctgggtga ggtcgccggt gttgacgccg gtcgctccgc
     7021 cggcgacgcc cgcgaccatc gccccgatcg cgaacaggtc ggtcttgcgc cgcatgtcgc
     7081 gccacatgcg gatcatgtgg cggcgcagga agtggccgga ctcgtgggca atcactccgg
     7141 ccagctgggc ctcgttgcgc atgcgcaaaa gcagcccgga gaagatcacc gtgaagccgc
     7201 tcgggaacat caccgcgttg aagtcgggga tccgcgccag atagatgcgc atgtccttgg
     7261 ccgccggccc gccgacggtg ccgatcaggt tctgcaggta gcggatcagc tccggatcct
     7321 tgatcagcaa gttcgagccc gcgacctcct cctcgacccg ggccatctgc tgccacaggc
     7381 cgatctcgtc gcggtcagtc ggccggtatc ccggcccgat cagcggccgc atgtcggctg
     7441 gccggatccg cgcctgggcg accccggtgg tcagcgaggc cgcggcgacc ccgccgccga
     7501 ccagcagcga gcggcgattg agctcatagc agcgaaagca catcagcgcc tctccatcgc
     7561 gcggcggacg tcccgccccg gcttcattcg gccgaccagg cgatcgacca gctgcgcggc
     7621 gccctgcggc gtgcggatgt cgcccatctt gatgccggcg atctggctgc cggtctggac
     7681 gacgttgaac cagaccactt cgccggtctt caggtcgacc agcgacgcgt aggcgaactg
     7741 cccgccgccg ccgatgttgg gcgcgcagaa gccgacgaag cagccggcga tcccgagcac
     7801 ctgcagcgcg acccggccgc cggagacgat attgtcctcg gcgaccatga acaaagcata
     7861 gtcgaagccg gtcttgcggc cgacctcgac cgcctgcgcg cccagcgtgt agtcgaggcc
     7921 gcggcgcggc ttggtcggca gataggcgcc gagatatttg tgcaaggcga tcgagttgcc
     7981 gaccgccgaa tgcagccgct cgaggtcggc gaccgtctcc tcgctgactc cggcaagctc
     8041 gttgcggcgg ccgaggatct gcaccttgcc gccgcgctcg gcctgctggg ccctgagggc
     8101 ggcggtgatg ttggcgcggg cctgctcggt ccagtcggcg cgcggctcgg gcaggccgcc
     8161 ggtggtgacc gaattgacgg tgacgtcggg ccgcatcacc agcagccggt aatcgccctg
     8221 cggcggagtg aactgcagat cggcgaactg gcgcgtctgg acgcacccgg cgaccggaac
     8281 gctgagcgcg agcgcgagcg cgattcccag atgacgcatg ttagacctcc gcgatccgca
     8341 aaccgcggcc gctccccaaa tgttccactg cggctccccg catgaacggc agatgaaaaa
     8401 acgccagtgt ttctagacga ttagcgcggc gggcaagcga cgccctgaat ccccgcgagg
     8461 catcggccgt cgatcgacgc ggaatcgacc cgaaggccga gcatcgccgc cagcgtcggc
     8521 aggatgtcga cggtctcgac cgcctcgttg cggtcggccg acgccatggc cgggcgccag
     8581 aacaggatcg gcacccgccg gtcgtagtcc cacgggctgc cgtgggtggc gatgtagcgg
     8641 ctggtgtcgg ggaccggggt cacgtgctgg cggagcagca cgacgaggtc gcccgagcgt
     8701 tcgaggtcga acgacgcccg cgctcgctcg gccaggctcc agcgctctgg gctggtcgtc
     8761 ggcaaggcga tccggcgaag ctggtcggcg gtgaacgccg tctccacctg cgggtgcgcg
     8821 gcataagcgg cgacggcttc gcgcaaggcc cgcgagcggt cggccggctt cagcgcccgg
     8881 tcgagataga cgtcgccgaa atagtcgccg atcagcaccg ggccggacag gccgagcttg
     8941 gcggcgatgc gcttgccgac cgctgtcgcc tcgagcgccg gatcgacccg aattgcagcc
     9001 ttcgcctcct gctggcgaag ccgctccggg atgtcctcga agccgtggtc ggcggtcagc
     9061 gcgaccgcat agtcgagccc gctgcggtcg agcagcttga agaaatcgcc gaggtcgcgg
     9121 tcgagcgaca gcagttgcag gcacatctcc tggccgcgga cgccgtacgt atggccgaca
     9181 taatcggtcg ccgacaggcc gaccgacaga agatcgggcg cggcgtcctg gccgagcttc
     9241 atgtcctgga tcagccccgc ggcaagggcg agcaccgcgc cgtcgaacgc gggcgaagcg
     9301 cgaaacgcgc tgccgtcgcc ggcggcccgc gcaaagcggc cgtcgccgac cggcttgccg
     9361 ccgccttcga ccggcaccgc ccgcgccttg gccgagcaca gctccggcgg ctcgagcggt
     9421 ggctgcgggg ccgcgatcag ggccccgacc gcggcgttgg cgcgggccac gctttgcgga
     9481 ggcgcggccc gaaggtcgct ggcgaagcgc ttgccgtccc aataccagcg ctggtcgacc
     9541 tggcggccgc ccatcatcac cgccgcccgg tccttgccgc cgacggcgac attgcggctg
     9601 gcgggcgatg aggccttgag ccgctcgccg agcgtcacgc ctttcaggtg caccggggac
     9661 accgtgtagg cggtggacga agaacccggg accgcggggt cctcggcgca atagaccgac
     9721 ttgtcggagc gggtcagcga ctggtcggtc cagtaattgg cgatgatccc ggtgcgcgcc
     9781 gggcgcaggc cggtgagcat ggtcgaatgg ccgggacagg tctcggtcgc ctcatgcccc
     9841 tggtagccgt tgcggaac
//
LOCUS       DASTZO010000174         4230 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_976662,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000174 DASTZO010000000
VERSION     DASTZO010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_976662"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VFU20_04890"
     CDS             <1..388
                     /locus_tag="VFU20_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285832.1"
                     /translation="GAFVPVEVTGDSTPELAVALGQLPCPAGAFRGPAGGTVQFWSTA
                     ARAPRLILEQQMHGFSPRGGLLVTLQHGGACGAASIARCLVTYRWDAGKRLMLAAARA
                     PVAEAEGLAAITFDQDLRRVAGATAQ"
     gene            complement(405..1070)
                     /locus_tag="VFU20_04895"
     CDS             complement(405..1070)
                     /locus_tag="VFU20_04895"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEU5285833.1"
                     /translation="MTEQHLALFRRHMVEIIDMHFDLAEDEIGKAAPDPRLRRALLDV
                     PRHLFVPQSLMVVAYQDTPLPIGFDKTISQPFIGALMLDLLGIEPGARILEVGTGLGY
                     QAAVMAEMGAQVWSVEVVEEFAEAASDRFAALGYNIEVRVGDGSRGWAEHAPFDAVLV
                     TAAAQAPPEALVEQLKPGGRMVIPVGGQDIQQLTVVTRQAGGGATERAIMPVRFTQLE
                     VTA"
     gene            1241..1513
                     /locus_tag="VFU20_04900"
     CDS             1241..1513
                     /locus_tag="VFU20_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285834.1"
                     /translation="MMPADFLTLIAATAIAQASGSEAALSVTATVVRPVQISAPAVDS
                     QGSVVTIRNTAGIEVRAEGATVRRIGPETVTVTGDGTGSMAIIIVY"
     gene            1614..2426
                     /locus_tag="VFU20_04905"
     CDS             1614..2426
                     /locus_tag="VFU20_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU5285835.1"
                     /translation="MTRYLLAATALATGLAAMATPAAARDRSAYFGLEVGALFVTDTD
                     VDLDDDEFFDDDFSIDHKMGVDGDMIAGYDFGMFRAEAELGYKRSEHDEYDGDSGAFD
                     ADGRSSVYSLMANAMVDLGPDEGFQFYAGAGIGYAQVIHVLEDGDDALKIKDRGFAWQ
                     LVAGVRKPVSQYFDLGLKYRYFDAGNVSDRDEGIELDSEYKSHSLLISLIHNFNVPLP
                     PPPPEPVVAPPPPPPAPPATQTCPDGSVILATDACPMPPPPPPPPPPEPERG"
     gene            complement(2493..3404)
                     /locus_tag="VFU20_04910"
     CDS             complement(2493..3404)
                     /locus_tag="VFU20_04910"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU5285836.1"
                     /translation="MRATVLINRGGGALAAGRRIDAEVETALASAGIAGDVEQLGGAE
                     LAGKARAAAQRGDPLLIVGGGDGSVSAAAGALAGTATRLGILPLGTLNHFARDLSIPS
                     DLGEAAAVIAAGSERSVDVAEVNGRTFINNAAIGVYPLMVVDRQSQQRRLGRSKWLAV
                     AVASLRTMWNFHAWRLRLSADGGEERVDTPLLFVGNNEYRLALPGAGRRESLEDGRLS
                     VLVMRSKSLPGFIAATARALLGIPRQDDMEHLEGITELRVDSARRRLPLGIDGETVTL
                     ETPLRFRIRPRALRVVAPAAAPGSSEA"
     gene            complement(3440..3961)
                     /locus_tag="VFU20_04915"
     CDS             complement(3440..3961)
                     /locus_tag="VFU20_04915"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEU5285837.1"
                     /translation="MRTTIILGLAAAGLLAACSGDTAQEPKKEAKAASLQPGEYEVTD
                     KVDELRSTDNSTPATATKAAAPGEAPPTARICVGADGALDPKIFAEAGDKCTPTGSYM
                     RGGRLSFQFNCTRPGKGGISQLVDGKFTADSFEASVTSSTYFSGPGDYSLTRTLTGRR
                     VGDCPAAPPPAAQ"
     gene            complement(3985..>4230)
                     /locus_tag="VFU20_04920"
     CDS             complement(3985..>4230)
                     /locus_tag="VFU20_04920"
                     /inference="COORDINATES: protein motif:HMM:NF023696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3617 family protein"
                     /protein_id="HEU5285838.1"
                     /translation="GRDKNCRYDHFTMGDGKIDAVMQCSGGGMAQTMAMQGSYGPDSY
                     RMRMSMKADAGTGPPGGMTMTMRVDARRVGECDSKKA"
BASE COUNT          586 a         1526 c         1464 g          654 t
ORIGIN      
        1 cggcgcattc gtgccggtcg aggtcaccgg cgattcaacg cccgagctcg ccgtcgcgct
       61 cggccaattg ccgtgcccgg ccggggcgtt ccgcgggccg gccggcggca ccgtccagtt
      121 ctggagcacg gcagcgcgtg cgccgcgcct gatcctcgag cagcagatgc acggcttttc
      181 gccgcgcggc gggctgctcg ttacgctcca gcacggcggc gcttgcgggg ccgcaagcat
      241 cgcccgctgc ctcgtcacct atcggtggga tgcgggcaag cggctcatgc tggccgcggc
      301 ccgcgccccg gtcgcggagg cggaggggct ggccgcgatc accttcgacc aggaccttcg
      361 tcgcgtggca ggcgccaccg cgcaatgact ggcgccgcga ccggctaggc cgtcacctcg
      421 agctgggtga agcggaccgg catgattgcg cgctccgtcg ctccgccgcc ggcctgcctg
      481 gtgaccacgg tcagctgctg gatgtcctgg ccgccgaccg ggatcaccat ccggccgccc
      541 ggcttcagct gctcgaccag cgcttcgggc ggcgcttgcg cggcggcggt gaccagcacc
      601 gcgtcgaacg gcgcatgctc ggcccagccg cgcgagccgt cgccgacccg aacctcgata
      661 ttgtagccca gagcggcgaa gcggtcgctc gcggcctcgg cgaattcctc gaccacctcg
      721 acgctccaca cctgggcgcc catctcggcc atcaccgccg cctggtagcc gagcccggtg
      781 ccgacctcga ggatccgggc gccgggctcg atgccgagca ggtcgagcat cagcgcgccg
      841 atgaacggct gcgagatcgt cttgtcgaag ccgatcggca gcggcgtgtc ctggtaggcg
      901 acgaccatca gcgattgcgg cacgaacaga tggcggggga cgtcgagcag cgcccggcgc
      961 aggcgcgggt cgggcgccgc cttgccgatc tcgtcctcgg cgaggtcgaa gtgcatgtcg
     1021 atgatctcga ccatgtgccg gcggaacagg gccaaatgct gttcggtcat cggcttcacg
     1081 ggggaaagcc ctcctccaac ccacgaacgg gcccggcaag ctcgcgttcc accatcgggc
     1141 gccggcggcg cccccgtgta gcggcgcacg acccgtccca atgctgaaca gcgccggaaa
     1201 acggtttggc ggaccggcgc ccagggccga tcagccggcg atgatgcctg ccgacttcct
     1261 gaccctgatc gcagcgacgg ccatcgcaca ggccagcggt agcgaagcgg ccctttcggt
     1321 caccgcgacg gtcgtccggc cggtccagat ctccgccccc gcggtcgact cgcaagggtc
     1381 ggtcgtcacc atccgcaaca ccgccggcat cgaggttcgg gcagagggcg cgaccgtccg
     1441 acggatcggg ccggagacgg tgaccgtcac cggcgacggc accgggtcga tggcgatcat
     1501 catcgtctat tgaagcaatg ggcaggcgcc tccagagtgc gtctcgacct ccgttaaact
     1561 ttgagttaat gggggtttgc ggctttgtcc gcccaaacag ggggtaagat aagatgacac
     1621 gctatttgct ggccgccacg gcgctggcca ccgggctggc ggcaatggcc acacccgcgg
     1681 ccgctcgcga ccgctcggcc tatttcgggc tcgaggtcgg ggcgctgttc gtcaccgaca
     1741 ccgatgtcga tctcgacgat gacgaattct tcgacgacga tttctcgatc gaccacaaga
     1801 tgggcgtcga tggcgacatg atcgccggtt acgatttcgg gatgttccgc gccgaggccg
     1861 agcttggcta caagcgcagc gagcatgacg aatatgacgg cgacagcggt gcgttcgacg
     1921 ccgacggccg gagcagcgtc tattcgctga tggccaatgc gatggtcgac ctgggcccgg
     1981 acgaaggctt ccagttctac gccggcgccg gcatcggcta cgcgcaggtg atccacgtcc
     2041 tcgaggacgg cgacgacgcc ctgaagatca aggaccgcgg cttcgcctgg cagctggttg
     2101 ccggggtgcg caagccggtg tcgcaatatt tcgacctcgg cctcaagtac cgctacttcg
     2161 acgccggcaa cgtcagcgac cgcgacgagg gcatcgagct cgacagcgag tacaagtcgc
     2221 actcgctgct catcagcctg atccacaatt tcaacgtgcc gctgccgccg ccgcctccgg
     2281 agccggtggt cgcgccgccg ccgccgccac ccgcgccgcc ggcgacccag acctgcccgg
     2341 acggctcggt gatcctggcg accgatgcgt gcccgatgcc gccgcctccg ccgccgccgc
     2401 cgccgccgga gcccgagcgc ggctgactcg aagccgcagc gaaggctgct tatccaagct
     2461 ccactcgccc ctcggggcgg gtggggcttt tttcatgctt ccgacgagcc gggcgcggcc
     2521 gccggggcga ccacccgcag cgcgcgcggg cggatccgga accggagcgg cgtttcgagg
     2581 gtcaccgttt cgccgtcgat cccgagcggc aggcgccgcc gcgcgctgtc gacccgaagc
     2641 tcggtgatcc cctcgagatg ctccatgtcg tcctggcgcg ggatcccgag caatgcgcgc
     2701 gcggtcgcgg cgatgaagcc cggcaggctc ttgctgcgca tgaccagcac cgacagccgc
     2761 ccgtcctcga gcgactcgcg gcggccggcg cccggcagtg cgagccggta ttcgttgttg
     2821 ccgacgaaca gcaacggcgt gtcgacccgc tcctcgccgc cgtcggcgct cagccgcagc
     2881 cgccaggcgt ggaagttcca catcgtccgc aggctcgcca ccgcgaccgc cagccacttc
     2941 gaccgcccga gccggcgctg ctgcgactgg cggtcgacga ccatcagcgg gtaaaccccg
     3001 atcgcggcat tgttgatgaa ggtccgcccg ttgacctcgg cgacgtcgac cgagcgctcg
     3061 ctgccggcgg cgatcacggc cgcggcctcg ccgagatcgg acggaatgga gaggtcgcgg
     3121 gcgaaatggt tgagggtccc caggggcaag atgccgagcc gggtcgcggt ccccgcgagc
     3181 gcgcccgcgg cggcgctgac gctgccgtcg ccgccgccga cgatcagcag cgggtcgccg
     3241 cgttgcgccg cggcgcgggc cttgcccgca agctccgcgc cgccgagctg ctcgacgtcg
     3301 ccggcgatgc ccgctgaggc gagcgcggtc tcgacctcgg cgtcgatccg gcggcccgcc
     3361 gcgagcgcgc cgccgccgcg gttgatcagg accgtcgccc gcacgccgct gctgctgcgt
     3421 caggcccgcg gcgaagcggt cactgcgcgg ccggcggcgg cgccgccgga cagtctccga
     3481 cccgcctgcc ggtcagggtg cgggtcagcg aatagtcgcc cggcccgctg aaataggtcg
     3541 agctggtgac gcttgcctcg aagctgtcgg cggtgaactt gccgtcgacc agctggctga
     3601 tcccgccctt gccgggtcgg gtgcagttga actggaagct caggcgcccg ccgcgcatgt
     3661 agctgccggt cggggtgcat ttgtcgccgg cctcggcgaa gatcttcggg tcgagcgcgc
     3721 cgtcggcgcc gacgcagatg cgggcggtgg gcggcgcttc gccgggcgcc gcggccttgg
     3781 tcgcggtcgc cggggtgctg ttgtcggtcg agcgcagctc gtcgaccttg tcggtgacct
     3841 catattcgcc cggctgcagc gaggcggcct tggcttcctt cttcggctcc tgggcggtgt
     3901 cgcccgagca ggcggcgagc agccccgcgg ccgccagtcc caagatgatc gtcgttcgca
     3961 tttgcgtccc ccctgttggt gccgtcacgc ctttttcgag tcgcactcgc cgacacgcct
     4021 ggcgtcgacc cgcatggtca tcgtcatccc gcccggtggg ccggtgccgg cgtcggcctt
     4081 catgctcatc cgcatccgat acgagtcggg gccgtagctg ccctgcatcg ccatcgtctg
     4141 ggccatgccg ccgccgctgc attgcatcac cgcgtcgatc ttgccgtcgc ccatggtgaa
     4201 atggtcgtag cggcaattct tgtccctgcc
//
LOCUS       DASTZO010000175         4672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1189506,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000175 DASTZO010000000
VERSION     DASTZO010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189506"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFU20_04925"
     CDS             complement(<1..647)
                     /locus_tag="VFU20_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HEU5285839.1"
                     /translation="MATGPRIAIDAMGGDSGPAAMLAGASRARRRDPSLQFLFFGDEK
                     PIRAELKKHRNLDSGVTVDHTPESIAPTEKPSQAIRRARTTSMGMAINAVKEGEADAA
                     LSAGNTGAMMAMAKLALRTMPGIDRPALAALLPTLGEHDCVMLDLGANTECDAQNLVQ
                     FAVMGSAYARTVLGIDKPRVKLLNIGTEELKGTDELKEAAGLLREADYLPLRFNG"
     gene            complement(677..856)
                     /gene="rpmF"
                     /locus_tag="VFU20_04930"
     CDS             complement(677..856)
                     /gene="rpmF"
                     /locus_tag="VFU20_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU5285840.1"
                     /translation="MAVPKRKTSPSKRNMRRSHHALKPASFQECPNCGELKLPHNLCA
                     ACGHYNGREVVSSEA"
     gene            complement(988..1650)
                     /locus_tag="VFU20_04935"
     CDS             complement(988..1650)
                     /locus_tag="VFU20_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5285841.1"
                     /translation="MSAPPLRAAIVPVTPLQQNCTLLWCTDTMLGAFVDPGGDLPRLK
                     AAADQAGVQIEKILLTHGHIDHCGSAGILAGELGVPIEGPHADDIFWIARLAEDGARY
                     GIAGKPFKPDRWLVDGDQASVGKLTFDVRHCPGHTPGHVVFHHPGSKLALVGDVLFKG
                     SVGRWDFPKGDQQQLIRSITTRLWPMGNETAFVPGHGPMSTFGHERATNPYVSDRALA
                     AA"
     gene            complement(1647..3566)
                     /locus_tag="VFU20_04940"
     CDS             complement(1647..3566)
                     /locus_tag="VFU20_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5285842.1"
                     /translation="MRPIRLALAAAAIALSAAAFAEVPKPAALTADGIPPVPGELAAT
                     SRPYMEFRSAGFAGWNPTDRSMLIATRFANTSQLHHVAVPLGMRRQISFEPEPVGGEW
                     SPKGDVLLVSKDVGGDEFYQLHTLANGRLRLLTDGKSRNEFDTWDKEGRLIGYSSTRR
                     NGTDTDLYVMDPRNPRTDRRVAQVKGGGWSIADFAPGGRRAAVLEYISITKSNPYLLD
                     IATGRLAPIGDHRKPIAYRDLQFAHDGTLWALSDEGSDVQRLGTLDPRTGRFRPVADA
                     GRWDIEGFEISEDGRTLAYVVNQAGISRLMLLDAATGAVRPVPGLPPGQISGLEFAPW
                     GPLGFTLSSAKSPADAFSLDPATLTITRWTESETGGLDPTVNVEPELVEVKSFDGEPV
                     SGFLYRPDARRFPGRRPLIVNIHGGPESQSRPGFQGRNNYLLNELGIAIFYPNVRGSS
                     GYGKRFVSLDNGPFQRENSVRDIGAFLDRLAGDRGIDSGRMAVTGGSYGGYMCYASAI
                     FYATRFRGALCNVAISNFVTFLENTQSYRRDLRRVEYGDERDPRQRAKLLEIAPLRRI
                     AEIRAPLFVVQGANDPRVPKSEADQIVRAVRARGNEVWYLVGENEGHGFGKKENQDYL
                     FWTTLMFWQKNLLAR"
     gene            complement(3597..>4672)
                     /locus_tag="VFU20_04945"
     CDS             complement(3597..>4672)
                     /locus_tag="VFU20_04945"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU5285843.1"
                     /translation="GLESGAAARDKPLMTDMARLRSGRVGGQFWSVYVTGTITGDAAL
                     RAVIEQVDVARRLIDAYPEHLELASSADDIVRIHRSGRIASLLGMEGGRGIAGSMPAL
                     RQLHNLGVRYMTLTHNQTTEWADSATDEPKHGGVSPFGAQVIAEMNRVGMLIDLSHVS
                     PEAMKDSLQMTRAPVIFSHSNAYALNPHPRNVPDEVLRLLPANGGVVMVNFFPLFLSP
                     EVREWALRRTAEEARLKSLLPFSAAAVEAAVKQWEAANPRPPVPVGLVADHIEHIARV
                     AGHAHVGIGGDLDGVPYTVVGLESVAGYPLLFAELIRRGWSDSNLARLAGGNVLRAMR
                     RAEAVAASMKDRPPSLAVLEPAK"
BASE COUNT          692 a         1568 c         1665 g          747 t
ORIGIN      
        1 aacccgttga agcgcagcgg caggtaatcg gcctcgcgca gcagcccggc ggcctccttg
       61 agctcgtcgg ttcccttcag ctcctcggtg ccgatgttga gcagcttgac ccgcggcttg
      121 tcgatcccga gcacggtccg ggcataggcc gagcccatga ccgcgaactg gaccaggttc
      181 tgggcgtcgc actcggtgtt ggcgcccagg tcgagcatca cgcaatcgtg ctcgcccaga
      241 gtcggcagaa gcgcggcgag cgccggccgg tcgatgcccg gcatggtgcg cagcgccagc
      301 ttggccatcg ccatcatcgc gccggtgttg cccgccgaca gcgccgcgtc ggcctcgcct
      361 tccttcaccg cattgatcgc catgcccatc gaggtggtcc gggcgcggcg gatcgcctgg
      421 ctcggcttct cggtcggggc gatggattcc ggcgtgtggt cgacggtgac gccggaatcg
      481 aggttgcggt gcttcttgag ctcggcgcgg atcggcttct cgtcgccgaa gaacaggaac
      541 tggagcgacg ggtcgcgccg ccgggcccgg gatgccccgg cgagcatcgc cgccgggccg
      601 ctgtcgccgc ccatggcgtc gatcgcgatt cgcggccccg ttgccatcgg cgagcgcccc
      661 ggctccggcc cgccgctcag gcctcgctcg aaaccacctc gcggccgtta taatggccgc
      721 aggcggcgca cagattgtgc ggcagcttca attcgccgca gttcgggcac tcctggaagc
      781 tcgcgggctt cagcgcatgg tggctgcgcc gcatgttgcg cttggagggc gaagtttttc
      841 tcttgggaac agccattgcg gcacctgctt cctgaacgaa taaaaattgc gacgatctgc
      901 gcctatctca acgggaaagc cccgcggcgg cggccggatc gtcgcgaacc gcggctccta
      961 taacgcaacg gcggtcgagc gcaagcttca ggcagccgcc agcgcccgat cgctcacata
     1021 aggattggtc gcgcgctcgt ggccgaaggt cgacatcggc ccatgccccg gcacgaaggc
     1081 agtttcatta cccatcggcc acagccgggt ggtgatcgac cggatcagct gctgctggtc
     1141 gccctttgga aagtcccagc ggccgaccga gcccttgaac aggacgtcgc cgaccagggc
     1201 caacttcgag cccggatggt ggaagacgac gtggcccggc gtgtggccgg ggcaatggcg
     1261 gacgtcgaag gtcagcttgc cgacgctcgc ctggtcgccg tccaccagcc agcggtcggg
     1321 cttgaacggc ttgccggcga tcccgtagcg ggcgccgtcc tcggcgagcc gggcgatcca
     1381 gaagatgtcg tccgcgtgcg gcccctcgat cgggaccccc agttccccgg cgaggatccc
     1441 tgccgagccg cagtggtcga tatggccgtg ggtcagcagg atcttctcga tctggacccc
     1501 ggcctggtcg gccgccgcct tcagccgcgg caggtcgccg ccgggatcga cgaacgcccc
     1561 gagcatcgta tcggtgcacc acagcaaggt gcagttctgc tgcagcgggg tcaccgggac
     1621 gatcgccgct cgaaggggcg gcgcgctcat cgggcgagca ggttcttctg ccagaacatc
     1681 agggtcgtcc agaacagata gtcctggttc tccttcttgc cgaagccgtg gccctcgttc
     1741 tcgccgacca ggtaccagac ctcgttgccg cgcgcccgga ccgccctgac gatctggtcg
     1801 gcctccgact tggggactcg cgggtcgttc gcaccctgga cgacgaacag cggggcgcgg
     1861 atctcggcga tccggcgcag cggcgcgatc tcgagcagct tggcgcgctg cctggggtcg
     1921 cgctcgtcgc catattcgac ccgccgcagg tcgcggcgat agctttgcgt gttctcgagg
     1981 aaggtgacga agttggagat ggcgacattg cacagcgcgc ccctgaatcg ggtcgcgtag
     2041 aagatcgccg aggcatagca catgtagccg ccgtagctgc cgccggtgac cgccatccgc
     2101 ccggaatcga tcccccgatc gccggccagc cggtcgagga aggcgccgat gtcgcggacc
     2161 gaattctcgc gctggaacgg cccgttgtcg aggctgacga agcgcttgcc gtagcccgac
     2221 gagccgcgga cgttcgggta gaagatggcg atcccgagct cgttgaggag atagttgttg
     2281 cgtccctgga agccgggccg cgactggctt tcggggccgc cgtggatgtt gacgatcagc
     2341 ggccgacggc cggggaaccg gcgcgcgtcg ggccgataga ggaagcccga caccggctcg
     2401 ccgtcgaagc tcttcacctc gaccagctcg ggctcgacgt tgacggtcgg gtcgaggcct
     2461 ccggtctcgc tttcggtcca gcgcgtgatg gtcagcgtgg ccggatcgag cgagaaggcg
     2521 tcggccgggc ttttcgccga gctgagcgtg aagccgagcg ggccccaggg cgcgaactct
     2581 agcccggaga tctgccccgg cgggagcccc ggcacgggcc ggacggcgcc ggtcgccgcg
     2641 tcgagcagca tcagccggct gatcccggcc tggttgacga cataagcgag tgtgcggccg
     2701 tcctcgctga tctcgaaccc ctcgatgtcc cagcggccgg cgtcggccac cggccggaaa
     2761 cggcccgtcc gcgggtcgag ggttccgagg cgctggacgt cgctgccctc gtcggacagc
     2821 gcccacaacg tcccgtcgtg tgcgaactgc aggtcacggt aagcgatcgg cttcctgtga
     2881 tcgccgatcg gcgcaaggcg gccggtggcg atgtcgagca aataggggtt gctcttggtg
     2941 atcgagatat attcgagcac ggcggcccgc cgcccgcccg gggcgaagtc ggcgatgctc
     3001 cagccgccgc ccttcacctg cgcgacccgg cggtcggtgc gcgggttgcg cgggtccatc
     3061 acatagaggt cggtgtcggt gccgttgcgc cgggtcgagc tgtagccgat cagccgccct
     3121 tccttgtccc aggtgtcgaa ctcgttgcgg ctcttgccgt cggtgagcag ccggaggcgg
     3181 ccgttggcga gcgtgtgcaa ctgatagaac tcgtcgccgc cgacgtcctt ggagaccagc
     3241 agcacatcgc ccttgggcga ccactcgccg ccgaccggct cgggctcgaa gctgatctgg
     3301 cggcgcatcc ccaagggcac cgcgacgtgg tgcagctggc tggtgttggc gaagcgggtc
     3361 gcgatcagca tcgaccggtc ggtcgggttc cagccggcga aaccggcgct tcggaactcc
     3421 atgtacggtc ggctggtcgc ggctagctcg ccgggaaccg gcgggatccc gtcggcggtg
     3481 agagcggcgg gcttcgggac ttcggcgaac gcggctgcgg acagggcgat ggcggcggcg
     3541 gcaagggcga gacggatcgg acgcaaggcg gaactccagc gtgaaacggg gatgggttac
     3601 tttgccggct cgaggaccgc cagcgacggc ggccggtcct tcatcgaagc ggcgacggcc
     3661 tcggcccggc gcatggcgcg cagcacgttg ccgccggcca gccgggcgag gttggaatcg
     3721 ctccagccgc ggcggatcag ctcggcgaac agcagcgggt atccggcgac gctttcgagc
     3781 ccgaccacgg tgtacggaac tccgtcgaga tcgccgccga tgccgacatg tgcatggccg
     3841 gcgacccggg cgatatgctc gatatggtcg gcgaccaggc cgaccgggac cggcggccgc
     3901 ggattggcgg cttcccattg cttcacggcg gcctcgaccg cggcggcgct gaacgggagc
     3961 agggacttca gccgcgcttc ctcggcggtc cggcgaagcg cccattcgcg cacctcgggc
     4021 gacaggaaca gcgggaagaa attgaccatc accaccccgc cgttggccgg cagcaggcgc
     4081 agcacctcgt cgggcacgtt gcgcgggtgc gggttcagcg cataagcgtt cgagtgcgag
     4141 aagatgaccg gagcgcgggt catctgcagc gaatccttca tcgcctcggg cgagacgtgg
     4201 ctgaggtcga tcagcatccc gacccggttc atctcggcga tgacctgggc cccgaacggg
     4261 ctgacgccgc cgtgcttggg ctcgtcggtc gcgctgtcgg cccactcggt cgtctggttg
     4321 tgggtcaggg tcatgtagcg cacgccgaga ttatggagct ggcgaagcgc cggcatcgac
     4381 ccggcgatcc cgcggccgcc ctccatcccg agcagcgagg cgatcctgcc ggagcggtgg
     4441 attcggacga tgtcgtcggc gctcgaggcg agctcgaggt gctccggata ggcgtcgatc
     4501 agccgtcggg cgacgtcgac ctgctcgatc accgcgcgca gcgcggcgtc gccggtgatc
     4561 gtgccggtga cgtagaccga ccagaactgg cccccgaccc gtccggagcg aagccgcgcc
     4621 atgtcggtca tcagcggctt gtcgcgcgcc gccgcgccgc tttcgaggcc gt
//
LOCUS       DASTZO010000176         2225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_615289,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000176 DASTZO010000000
VERSION     DASTZO010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2225
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_615289"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..647)
                     /locus_tag="VFU20_04950"
     CDS             complement(<1..647)
                     /locus_tag="VFU20_04950"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEU5285844.1"
                     /translation="MARVFLSYDREDEEKARLLAGAIEKAGHSVWWDRHIRSGAQYSK
                     EIEQALRTADALVVLWSKRSVDSPWVRDEAAAGRDSGRLIPVRLDSNEPPLGFRQFQT
                     IDLSRWKGRGKPAQLPTLLADIDALATGSSATPPAAPVPAERPARRRPTRRLVAVALL
                     IAALLVAGLVAWRLIGKPSPIPLVAVAATETSPASTALARDLFVQLGRLQATNADA"
     gene            788..1510
                     /gene="pyrH"
                     /locus_tag="VFU20_04955"
     CDS             788..1510
                     /gene="pyrH"
                     /locus_tag="VFU20_04955"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU5285845.1"
                     /translation="MTRPRFNRILLKLSGEALMGQGQLGIDPDATAGLAGEIAAAKAQ
                     GHELCVVVGGGNIFRGVAAAARGFDRASADYMGMLATVMNALALQNALEKAGVDTRVQ
                     SAIPMASVSEPYIRRRALRHIEKGRIVLFAAGTGNPYFTTDTAAALRAAEMGCDALFK
                     GTSVDGVYTADPKKEKGATRYDHLSYSKVLGDDLKVMDAAAVALCRDNNIPIVIFNIR
                     QPGNLAEVLAGRGTATIVQNEE"
     gene            1514..2074
                     /gene="frr"
                     /locus_tag="VFU20_04960"
     CDS             1514..2074
                     /gene="frr"
                     /locus_tag="VFU20_04960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU5285846.1"
                     /translation="MTTTVDKSDLQRRMHGALEVLKHDLGGLRTGRASTALLDPIQVE
                     VYGSQMPLNQVSTVSVPEPRMLTVQVWDRSNVQPVEKAIRSAGLGLNPITDGQLIRLP
                     IPDLTEERRKELAKLVGQYAEKARIAVRNVRRDGMDHLKHDERKHEISEDERKRLEHE
                     VQKLTDQTIAEIDAAAEAKEAEILGR"
BASE COUNT          393 a          776 c          740 g          316 t
ORIGIN      
        1 gcatcggcat tcgttgcctg cagcctgcca agctggacga acaggtctcg agccagcgca
       61 gtgctggccg gcgaggtttc cgtcgcagca accgcgacca gcggaatcgg ggacggcttc
      121 ccgatcaatc gccaggcgac caggcccgcg acaagcagcg ccgcgatcaa cagggccact
      181 gcgaccaggc gacgagtcgg ccgcctgcgg gccgggcgct cggccggcac cggtgcggcc
      241 ggcggcgtcg cgcttgatcc cgtcgccagc gcgtcaatgt ccgcgagcag ggtaggcaat
      301 tgcgcgggct tgccgcggcc tttccagcgc gacaggtcaa tcgtctggaa ctggcggaag
      361 ccaagcggcg gctcgttcga gtccaggcga accggaatca gccgcccgct gtcgcggcct
      421 gctgccgcct cgtcgcgaac ccaggggctg tccaccgatc gcttcgacca gagcacgact
      481 agtgcgtccg ccgtcctcag ggcttgctcg atttctttcg aatattgcgc tccgctgcgg
      541 atgtgccggt cccaccagac cgaatggccg gccttttcga ttgctcccgc cagcaaccgc
      601 gctttttcct cgtcctcgcg gtcataactc aggaacacgc gcgccatcgt ttgccccttt
      661 gcggccgatg ctgcttgcaa atggctgccg acgcaagcag cggggcaggt caaggcagtt
      721 ggcaacgcgc cggctgggcc ataagagcag ggccgccctt tccgcatccc aaggatgacc
      781 cgcccgaatg acccggccgc gcttcaatcg aatcctgctg aagctctccg gcgaggcgct
      841 gatggggcag ggccagctcg ggatcgatcc cgacgccacc gccggcctgg ccggcgagat
      901 cgccgccgcc aaggcgcagg gccacgaatt gtgcgtcgtc gtcggcggcg gcaacatctt
      961 ccgcggcgtc gcggccgccg cgcgcggctt cgaccgcgcc agcgccgatt acatgggcat
     1021 gctggcgacg gtgatgaacg cgctcgcgct gcagaacgcg cttgaaaaag ccggggtcga
     1081 cacgcgcgtc cagtcggcga tcccgatggc gtccgtcagc gaaccctaca ttcgccgccg
     1141 ggcgctccgg cacatcgaga agggacggat cgtgctgttc gcggccggca ccggcaaccc
     1201 ctatttcacc accgacaccg ccgcagccct gcgtgccgcg gaaatgggct gcgacgctct
     1261 gttcaagggc accagcgtcg acggcgtcta caccgccgac ccgaagaagg aaaaaggcgc
     1321 cacccgctac gatcatctga gctacagcaa ggtgctcggc gatgatttga aggtgatgga
     1381 cgccgccgcg gtggcgctgt gccgcgacaa caatatcccg atcgtcatct tcaacatccg
     1441 ccagccgggc aacctcgccg aggtgctggc cgggcgggga acggcgacga tcgtccagaa
     1501 cgaggagtga gcgatgacta cgaccgtcga caagtcggac ctgcagcgcc ggatgcacgg
     1561 cgctctcgag gttctcaagc acgatctcgg cgggcttcgc accggccgcg cctcgactgc
     1621 tctgctcgat cccatccagg tcgaggtcta cggctcgcaa atgcccttga accaggtgtc
     1681 cacggtgtcg gtgcccgagc cgcggatgct gaccgtgcag gtgtgggacc gatcgaacgt
     1741 ccagccggtc gaaaaagcca tccgctcggc cgggctcggc ctcaacccga tcaccgacgg
     1801 ccagctgatc cggctgccga tccccgattt gaccgaggag cggcgcaagg agctcgccaa
     1861 gctggtcggc caatatgccg aaaaggcgcg gatcgcggtg cgcaacgtgc gccgcgacgg
     1921 aatggaccat ctcaagcacg acgagcgcaa gcacgagatc agcgaggacg agcgcaagcg
     1981 gctcgagcat gaggtccaga agctgaccga ccagaccatc gccgagatcg acgccgccgc
     2041 cgaggccaag gaagcggaaa tcctcggcag gtgacccggg cgcgcaccgc ggcaccggac
     2101 gaggcggacg ggtccgcagg cgtcgcctcg cccggcggcg agggcgtccc gcgccacgtc
     2161 gcgatcatca tggacggcaa tggccgctgg gccgaccagc gcggcctgcc gcgggtcgcc
     2221 ggcca
//
LOCUS       DASTZO010000177         2790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1290331,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000177 DASTZO010000000
VERSION     DASTZO010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290331"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VFU20_04965"
     CDS             complement(<1..519)
                     /locus_tag="VFU20_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007703977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU5285847.1"
                     /translation="METRSNHVLVGAVVLAMLAGLLLFTVWLAGLSNKTTRCFDIYFA
                     QAVGGLNKGSTVSFSGVPVGQIEQISLLPDRPEFVWVRINVDEETPVLQGTTAEIKGV
                     GFTGVSEIQLTGAVKGGRPIRQPGPQGCPVIPSSSGGLGALLNSAPELIDRIQRLTER
                     LTELLSDENQNSI"
     gene            complement(519..1307)
                     /locus_tag="VFU20_04970"
     CDS             complement(519..1307)
                     /locus_tag="VFU20_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5285848.1"
                     /translation="MSGAPIITVRGLRNSFGEEVVHEGLDLDVERGEILGVVGGSGTG
                     KSVLMRSIIGLQTPDAGEIEVLGESMVGRDEDEAKNIRRRWGILFQNGALFSTLTVAE
                     NVEVPIHEYFPYLKPPLLDEIASYKIAMSGLPPDAGPKYPAELSGGMRKRAGLARALA
                     LDPELLFLDEPTAGLDPIGAAAFDELIQALKKRLELTVFLITHDLDTLHTICDRVAVI
                     ADQKVVAVGTIPELLSLEHPWIQEYFNGPRGRAAAGSYKEHVEA"
     gene            complement(1304..2422)
                     /locus_tag="VFU20_04975"
     CDS             complement(1304..2422)
                     /locus_tag="VFU20_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5285849.1"
                     /translation="MADEDQTAGESRATGHGAGQTYRIAGALTITRAASTAREIDALA
                     DPLTIDLSKVEKMDTVGAWIVYRTVRDRNAKVVGASAEEQSLLDQVAEADKPVQVHAE
                     KAGGFFHVLAELGAWINELGRTLIGLLAFFGATLAAFANLIAHPKRFRVNAVIQRFDV
                     VGVRALGIIGLMSFLIGVVVGQQGAVQLEQFGAEVFTINLIGRITVRELGTLMTAIMV
                     AGRSGSAFAAQIGTMKITEEIDAMRTIGVSPIEALVIPRLIAAVVMMPLLAFWAMLMS
                     LLGGGIFVWFELGIPPLTYIQRLQEVVPITDLWIGLIKAPVFGFIIALAGCFQGMLVQ
                     GNSEEVGLRTTTAVVQSIFLVIVLDAMFAVFFSSIGWK"
     gene            2551..>2790
                     /locus_tag="VFU20_04980"
     CDS             2551..>2790
                     /locus_tag="VFU20_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU5285850.1"
                     /translation="MSSDPPPLFVPVGSNPARLFGLDARARACRLAANAGFECAETPQ
                     AGRGAILASLDYAWDPAWLAEIAGRPGSLLTLGGRP"
BASE COUNT          430 a          953 c          945 g          462 t
ORIGIN      
        1 gatcgaattc tggttctcgt cgctcagcag ctcggtcagc cgctcggtca gccgctggat
       61 tcggtcgatc agctcggggg cgctgttgag aagcgcgcca agcccgcccg agctcgacgg
      121 gatcaccggg cagccctgcg gcccgggctg gcggatcggg cggccgccct tgaccgcccc
      181 ggtcagctgg atctcgctga ccccggtgaa gccgacgccc ttgatctcgg cggtggttcc
      241 ctggagcacc ggggtctcct cgtcgacgtt gatccgcacc cagacgaact cggggcggtc
      301 ggggagcagg gagatctgct cgatctggcc gaccgggacg ccagagaagc tgaccgtcga
      361 gcccttgttc agcccgccga ccgcctgcgc gaagtagatg tcgaagcagc gcgtcgtctt
      421 gttggacagg ccggccagcc agacggtgaa cagcagcagc cccgcgagca tcgcgaggac
      481 gactgcaccg accaggacgt gattggaccg cgtttccatc aggcttcgac gtgttccttg
      541 tagctgcccg ccgcggcgcg tccacgcggg ccgttaaaat attcttgaat ccaaggatgt
      601 tccaaagaca atagttccgg aatcgttccg accgccacta ctttttgatc ggcgatgacc
      661 gcgacccggt cgcagatggt gtgcagcgtg tcgaggtcgt gggtgatcag gaacacggtc
      721 agctcgagcc gtttcttcag cgcctggatc agctcgtcga aggcggcggc gccgatcggg
      781 tcgaggccgg cggtcggctc gtcgaggaac agcagctccg ggtcgagcgc cagcgcccgg
      841 gcgagcccgg cgcgcttgcg catcccgccc gacagctcgg ccgggtattt gggcccggcg
      901 tcgggcggca ggccgctcat cgcgatcttg taggacgcga tctcgtcgag cagcggcggc
      961 ttcagatagg ggaaatattc gtggatcggg acctcgacgt tctcggccac ggtcagggtc
     1021 gagaacaagg ctccgttctg gaacaatatc ccccagcgcc ggcggatgtt cttcgcctcg
     1081 tcctcgtcgc ggccgaccat gctctcgccg agcacctcga tctcgcccgc gtcgggcgtc
     1141 tgaaggccga tgatcgagcg catcagcacc gactttccgg tgcccgatcc gccgaccacg
     1201 ccgaggatct cgccgcgctc gacgtccagg tcgaggccct cgtggaccac ctcctcgccg
     1261 aagctgttgc gaagcccgcg gacggtgatg atcggcgccc cgctcatttc cagccgatcg
     1321 agctgaagaa caccgcgaac atcgcgtcga ggacgatgac caggaagatc gactgcacga
     1381 ccgccgtggt ggtgcgcagc ccgacctcct cgctgttgcc ctggacgagc atgccctgga
     1441 agcagccggc gagcgcgatg atgaagccga acacaggcgc cttgatgagg ccgatccaca
     1501 aatcggtgat cgggaccacc tcctgcagcc gttggatgta ggtcagcggc gggatgccga
     1561 gctcgaacca gacgaagatg ccgccgccga gcagcgacat cagcatcgcc cagaaggcga
     1621 gcagcggcat catgacgacc gccgcgatca gccgcgggat gaccagcgcc tcgatcggcg
     1681 acacgccgat ggtgcgcatc gcgtcgatct cctcggtgat cttcatcgtc ccgatctggg
     1741 cggcgaacgc cgatcccgag cggccggcga ccatgatcgc ggtcatcagc gtgccgagct
     1801 cgcggaccgt gatccggccg atcaggttga tggtgaagac ctccgccccg aactgctcga
     1861 gctgcaccgc cccctgctgg ccgacgacga ccccgatcag gaagctcatc aggccgatga
     1921 ttcccagcgc ccggaccccg acgacgtcga agcgctggat gaccgcgttg acccggaacc
     1981 gcttcgggtg ggcgatcagg ttggcgaagg cggcgagggt cgctccgaag aaggcgagca
     2041 ggccgatcag ggtccggccg agctcgttga tccacgcgcc cagctcggcg aggacgtgga
     2101 agaagccgcc cgctttctcg gcgtggacct ggaccggctt gtcggcctcg gccacctggt
     2161 cgagcaggct ctgctcctcg gcgctggcgc cgaccacctt ggcgttgcgg tcgcggacgg
     2221 tgcgatagac gatccacgcc ccgacggtgt ccattttctc gaccttggac aggtcgatgg
     2281 tcagcgggtc ggcgagcgcg tcgatctcgc gggcggtgct ggcggcgcgg gtgatggtga
     2341 gggcgccggc gatgcgatag gtctggccgg cgccgtgccc ggtcgcgcgg gattccccgg
     2401 cggtttgatc ctcgtcggcc atttgggttg gattagtgcg cgaattcctt cacctcggca
     2461 agtatcgccc ggtcgaggcc cggccttgcc cgccgccact ttcctgccgc ccctttcctg
     2521 ccgtcattgt cccctctaag cgctcgggcc atgtcgagcg acccgcctcc cttgttcgtg
     2581 cccgtcgggt cgaatccggc gcggctgttc gggctcgacg cgcgcgcccg cgcctgccgc
     2641 ctcgcggcca acgccggttt cgagtgcgcc gagacgccgc aggccggccg cggagcgatc
     2701 ctcgccagcc tcgactatgc gtgggacccg gcctggctgg cggagatcgc cgggcgcccc
     2761 ggaagcctgc tcacgctcgg cggccggccg
//
LOCUS       DASTZO010000178         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1290427,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000178 DASTZO010000000
VERSION     DASTZO010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1290427"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1041
                     /locus_tag="VFU20_04985"
     CDS             <1..1041
                     /locus_tag="VFU20_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5285851.1"
                     /translation="GVNTRTNSRGTMFTTTGPEDVDFSNEYTDWLPNANMTVRFSPEW
                     QLRLAATQTRTRPTFQQLNPALSLGSPPNCASLPPGVDCIRTGSGGNPFLKPLDSNNY
                     DASIEYYFSRNGFASAAVFHRDMRGFIANRAVEFPDPDPETGLPLVISGPANTRKGKI
                     RGFEAQVSSFFDWEFVPTWARAFGAQANVTYIDAKADFSLFCPPTAETCEPQPGAPNA
                     TIRRLRIPDVSKWTYNLVGMYEAGGLTLRLAYNRRSKFPEGGLAERDGFFTLQGRGQP
                     VSRLDWSSSYTVTDNVTVFFDWTNILKKPFKSDIVRVNYAAGEPVGDPEIFPMVARFE
                     ESIMTGGVRFRF"
     gene            complement(942..2312)
                     /locus_tag="VFU20_04990"
     CDS             complement(942..2312)
                     /locus_tag="VFU20_04990"
                     /inference="COORDINATES: protein motif:HMM:NF022623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 172 protein"
                     /protein_id="HEU5285852.1"
                     /translation="MRPLLALAALLLGSQALAQDRSDLYRYRADERTHWVSPENPTGA
                     KGAGALENKGAKGHAFETIPVGASHVLADIRGAGTIDRMWMTIEDRSPEALRGLKLEM
                     FWDGASAPAVSVPLGDFFLHGAGEMVAMETALFASPEGRSFVSYVPMPFRKRARLVVT
                     NESPRPVNLIFFDINYRAVPSQPGDALYFHAWWSRDRATKLGQDFRILPRIAGRGRFI
                     GAGVTVLTNPVYEKTWWGEGEVKIALDGDRRDAPSLVGTGAEDYIGTAWGQGAYVNRF
                     QGAPVATWDGEGRWTFYRFHIPDPVWFHRSIEVSLQQIGGARKAIVLGLLDKGAPLIP
                     VTIDPGSRTNFQKLLESGKALSDPSLPDGHVNFYRSDDVAAVAYFYLDRPGGVLPPIA
                     PAAERLAAIRHPPKKIRAAVAGRPRSVRVELGQKRKRTPPVMIDSSNRATIGKISGSP
                     TGSPAA"
BASE COUNT          397 a          828 c          810 g          388 t
ORIGIN      
        1 ggggtcaaca cccggaccaa cagccggggc accatgttca ccacaaccgg gcccgaggac
       61 gtcgacttca gcaacgaata taccgactgg ctgccgaacg cgaacatgac ggtccgcttc
      121 agtcccgagt ggcaattgcg gctcgcggcg acgcaaaccc ggacccggcc gacgttccag
      181 cagctcaacc cggcgctgtc gctcggaagc cctcccaatt gcgccagcct tccgcccgga
      241 gtcgactgca tccggaccgg cagtggcgga aacccgttcc tcaagccgct cgattccaac
      301 aattacgacg cgagcatcga atattatttc tcgcgcaacg gctttgcctc ggctgcggtg
      361 ttccaccgcg acatgcgggg attcatcgcc aaccgcgcgg tcgagttccc tgaccccgac
      421 cccgagaccg gtctgccgct ggtgatcagc ggcccggcca acacgcgcaa gggcaagatc
      481 cggggcttcg aggcgcaggt cagcagcttc ttcgactggg agttcgttcc gacctgggca
      541 cgcgccttcg gcgcccaggc gaacgtcacc tacatcgacg ccaaggccga cttctcgctg
      601 ttctgcccgc cgaccgccga gacctgcgag ccccagccgg gcgccccgaa cgcgaccatc
      661 cgccggctgc gcattccgga cgtctcgaag tggacctaca acctagttgg aatgtacgag
      721 gcgggcgggc tgacgcttcg gctcgcctac aaccgccgaa gcaagtttcc cgagggcggc
      781 ctggcggaac gcgacggctt cttcaccctc cagggccgcg gccagccggt ctctaggctc
      841 gactggtcga gcagctacac ggtcaccgac aatgtcacgg tcttcttcga ctggaccaac
      901 atcctcaaga agccgttcaa gtcggacatc gtgcgggtca attatgcggc cggcgagcct
      961 gtcggcgatc cggaaatctt cccgatggtc gcccggttcg aggaatcgat catgaccggc
     1021 ggggtccgct tccgcttctg accgagctcg acacgcacac ttctagggcg gcccgcaacg
     1081 gccgccctta tttttttggg cgggtggcgg atcgcggcca ggcgctcggc ggccggagcg
     1141 atcggcggca gcactccgcc cgggcggtcg aggtagaaat aagcgaccgc ggcgacgtcg
     1201 tcgctgcgat agaaattgac gtggccgtcg gggagcgacg ggtcggacag cgccttgccg
     1261 ctttcgagca atttctggaa attggtgcgg ctgcccgggt cgatggtcac cgggatcagc
     1321 ggcgcgccct tgtcgagcag gccgaggacg atggctttgc gcgcgccgcc gatctgctgg
     1381 agcgagacct cgatggagcg gtggaaccac accgggtcgg ggatatggaa gcggtagaag
     1441 gtccagcgcc cctcgccgtc ccaggtggcg accggagcgc cctggaagcg gttgacgtag
     1501 gcgccctgcc cccaggcggt gccgatataa tcctcggccc cggtgccgac cagggacgga
     1561 gcgtcgcggc ggtcgccgtc gagcgcgatc ttgacctcgc cctcgcccca ccaggtcttc
     1621 tcgtacaccg ggttggtgag cacggtgacg ccggcgccga tgaagcgccc gcggccggcg
     1681 atgcgcggca ggatgcggaa atcctggccc agtttcgtcg cccggtcgcg gctccaccag
     1741 gcgtggaagt aaagcgcgtc gccgggctgg ctcggcacag cgcggtagtt gatgtcgaag
     1801 aagatcaggt tgaccggcct gggcgattca ttggtgacca ccagccgggc tctcttgcgg
     1861 aacggcatcg gcacatagga cacgaacgag cggccctcgg ggcttgcgaa cagcgccgtc
     1921 tccatcgcca ccatctcgcc ggcgccgtgg aggaagaagt cgccgagcgg caccgacacg
     1981 gcgggcgccg acgctccgtc ccagaacatc tcgagcttca ggccgcgcag cgcctcgggc
     2041 gagcggtcct cgatggtcat ccacatccgg tcgatggttc cggcaccccg gatgtcggca
     2101 aggacgtggc ttgcgccgac gggaatggtc tcgaacgcgt ggcccttggc gcccttgttc
     2161 tcgagcgccc cggcgccctt ggcgccggtc gggttctcgg ggctgaccca gtgcgtgcgc
     2221 tcgtcggcgc ggtagcggta gaggtcggaa cgatcctgcg ccagcgcctg cgaccccagc
     2281 agcaaggcgg cgagcgcgag gagcggcctc acccccgctc gctcactttg cgcgcggccg
     2341 caggctcggc gcgagcttgg gcttgccgtc cttccagaac atcggcgcga tgtagaggta
     2401 gcgcttgtcg tcggccttgc ggc
//
LOCUS       DASTZO010000179         3731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_351344,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000179 DASTZO010000000
VERSION     DASTZO010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351344"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1339)
                     /gene="der"
                     /locus_tag="VFU20_04995"
     CDS             complement(<1..1339)
                     /gene="der"
                     /locus_tag="VFU20_04995"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEU5285853.1"
                     /translation="MSFTVAIVGRPNVGKSTLFNRLVGRRLALVDDQPGVTRDRREGR
                     AQLGDLSFTVIDTAGLDEAAPASLTGRMQAQTAAAVAGADAVMFVIDARAGLLPADEA
                     FADFVRRSGKPVIVVANKSEGRATQAGANEAYALGLGEPVAISAEHGEGLADLYEALR
                     AVAPAEAEDVAIEKEEGAVVAPAEPAPIRIAVVGRPNSGKSTLINRLLGEERLLTGPE
                     AGITRDAIAVDLAWHSTRFRVHDTAGMRKRARIDEKLEKLSVADALNAIRFAEVVILL
                     LDAERPFEEQDLRIADLIEREGRALVIGMNKWDRKESVPGAMTRLREETDHWLPQVKG
                     VPVVAVSGLTGYGLDRLMQAVLDAHAVWNKRVPTAALNRWLGEVISAHPPPAVSGRRV
                     RIDYLTQPKARPPSFVLFMSRTDALPDAYRRYLVNALREQTDHWLPQVKGVPVV"
     gene            complement(1398..1898)
                     /locus_tag="VFU20_05000"
     CDS             complement(1398..1898)
                     /locus_tag="VFU20_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285854.1"
                     /translation="MNRFARATLAASALSLGLMLGACGTMDTFDPTDLFSAEIFNTKK
                     KLPGERKPVFPEGTPGVPQGVPAELVKGHQPAAEPEVTQAIPEQKEAAAEPEKPKAKP
                     KPKPKVVAKPQPPEEKPTSVTVNRSESPWPDPPQASTQPQASAQPQSAWPGAQRGGSI
                     WPDPPR"
     gene            complement(1911..2426)
                     /locus_tag="VFU20_05005"
     CDS             complement(1911..2426)
                     /locus_tag="VFU20_05005"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5285855.1"
                     /translation="MWEAKKAAEAGAAFEAAIALSDGGKHAEAEAAFAKIAGDSTSGY
                     RQLARIREAAELVYQDPKAAVAAFEKIANDGAADPVLRDLAALRAGALSIDAGSYPDA
                     QRLLEPLAQPDRTFRHTARELLALSAWRQGDGPGTKRWFDMLATDPLTPAATRSRAEM
                     LMALVAAEAKS"
     gene            complement(2668..3516)
                     /gene="panB"
                     /locus_tag="VFU20_05010"
     CDS             complement(2668..3516)
                     /gene="panB"
                     /locus_tag="VFU20_05010"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU5285856.1"
                     /translation="MSVQEEIKRLTAPDIRARKGGEPIVSLTSYHAHTARLLDAHCDV
                     ILVGDSLGMVMHGLESTVPVTLDMMILQGHAVMRGSKRALVVIDMPFGSYEASKEQAF
                     MSAARVMKETGCGAIKLEGGRRMAETIRFLAERGVPVMGHIGLTPQSINTIGSFRAQG
                     RDEADWAPIEDDARAVSDAGAFSVVIEAVAEPLARKITADVPIPTIGIGGSVACDGQI
                     LVLEDMLGLTPRVPKFVRRYGELGPSIGQAVAAYAADVRARAFPGPEHVYPMRTKGKS
                     GGPKAG"
BASE COUNT          554 a         1290 c         1262 g          625 t
ORIGIN      
        1 cgaccaccgg cacgcccttg acctgcggca gccaatggtc ggtttgctcg cgcagcgcat
       61 tcaccagata gcgccggtag gcgtcgggca gcgcgtcggt gcggctcatg aacagcacga
      121 agctcggcgg acgcgccttc ggctgtgtga ggtagtcgat ccggacccgc cggcccgaca
      181 ccgcaggcgg cggatgcgcc gagatcacct cgccgagcca acggttcagc gcagcggtcg
      241 gcacccgctt gttccacacc gcgtgagcgt cgagcaccgc ctgcatcagc cggtccagtc
      301 cgtacccggt gaggccggac accgcgacca ccggcacgcc cttgacctgc ggcagccaat
      361 ggtcggtttc ttctcggagc ctcgtcatcg caccgggcac gctttccttg cggtcccact
      421 tgttcatgcc gatgaccagc gcgcggcctt cgcgctcgat caggtcggcg atgcgcaggt
      481 cctgctcctc gaacgggcgc tcggcatcga ggagcaggat caccacctcg gcaaagcgga
      541 tggcgttgag cgcgtcggca accgagagct tctcgagctt ctcgtcgatg cgcgcgcgct
      601 tgcgcatgcc ggcagtatcg tggacgcgaa agcgcgtgct gtgccaggcg agatcgaccg
      661 cgatcgcatc gcgggtgatg ccagcctccg gcccggtgag caggcgctcc tcgccgagca
      721 gccggttgat caacgtcgac ttgccggaat tcggccggcc caccaccgca atgcggatcg
      781 gcgccggttc ggcgggcgct acgacggcgc cctcctcctt ttcgatcgcc acatcttcgg
      841 cttccgccgg tgcgacggcc cgcagcgcct cgtagaggtc ggcgaggcct tcgccatgct
      901 cggccgagat tgcgaccggc tcaccgagac cgagcgcata ggcctcgttg gcgccggcct
      961 gcgtcgcccg gccttcgctc ttgttggcga ccacgatcac cggcttgccc gagcggcgca
     1021 cgaaatcggc gaacgcttca tccgccggca gcagcccggc gcgcgcgtcg atcacgaaca
     1081 tcacagcgtc ggcgcccgcg accgcggccg cagtctgcgc ctgcatgcgc ccggtaaggc
     1141 tcgccggcgc agcctcgtcg aggcctgcgg tatcgatcac cgtgaaggag agatcgccaa
     1201 gttgcgcgcg gccctcgcgg cggtcgcggg tgacgcccgg ctggtcgtcg accagcgcga
     1261 ggcgccggcc gaccagccgg ttgaacaggg tcgacttgcc gacgttcggc cgtccgacga
     1321 tggcgaccgt gaagctcatg cgggatgtat ggcgcgtatg cttcgcgcgc gcgagagcgt
     1381 tgcgatggaa agttcggtca gcgcggcgga tcgggccaga tcgacccgcc ccgctgcgcg
     1441 cccggccagg cggactgcgg ctgggcggag gcctgcggct gcgtcgacgc ctgcggcgga
     1501 tcgggccagg gcgattcgga ccggttcacg gtgacggagg tcggcttctc ttccggcggc
     1561 tgcggcttgg cgaccacctt cggtttcggc ttgggcttcg ccttgggctt ttcgggttcg
     1621 gccgcggcct ctttctgctc cggaatggcc tgggtgactt ccggctccgc ggccggctgg
     1681 tgacccttga ccagttcggc cggcacgcct tgcggcacgc cgggcgtgcc ctcggggaac
     1741 accggcttgc gctcgcccgg cagcttcttc ttcgtgttga agatttcggc gctgaagaga
     1801 tcggtcggat cgaaggtatc catcgttccg caggccccca gcatcaggcc gagcgagagc
     1861 gccgatgcgg cgagcgtggc acgagcgaag cggttcatgg tttcgccttc tcagctcttg
     1921 gcctcggccg cgacgagagc catcagcatc tcggcgcgcg agcgggttgc ggctggcgtc
     1981 aacggatcgg tcgcgagcat gtcaaaccag cgcttggtgc cgggaccgtc gccctggcgc
     2041 caggcggaaa gcgcgagcag ttcgcgcgcg gtgtggcgga aggtgcggtc cggctgggcg
     2101 agcggctcca gcagccgctg tgcgtcggga tagctgccgg catcgatgga gagtgcgccg
     2161 gcgcgcaacg cagcgagatc gcgcagcacc ggatcggcgg cgccatcgtt ggcgatcttc
     2221 tcgaacgccg ccaccgcggc cttgggatcc tggtagacga gttccgcggc ttcgcggatg
     2281 cgcgcgagct gccggtagcc ggacgtgctg tcgcccgcga tcttggcgaa tgccgcctcg
     2341 gcttcggcat gcttgccgcc gtcgctgagc gcaatcgccg cctcgaacgc agcgccggcc
     2401 tcagccgcct ttttggcttc ccacattggt agccgcgcca ggcgccgacg gccgcgacga
     2461 ccagaaccgc cagcacgacg acaaagatgc cgtagcgatc ccacagcttt ttgagctgct
     2521 cgcggcggac ttcctcgtcg acttcgcgga agacgtctac gtcggacacg taaattcccc
     2581 tgctccaggc cccccagacg gcctaggctg acatcgcggg taacctagcg atgtggcaga
     2641 gccaaggcaa agattaacgt agtgtgtcta acccgctttg ggtccgccgg atttcccttt
     2701 tgttctcatc ggatagacgt gctccggccc tgggaatgcc cgggcgcgca cgtcggccgc
     2761 gtaggcggcc accgcctggc cgatcgaggg cccgagctcc ccatagcggc ggacgaattt
     2821 cggcacccgc ggcgtcagcc cgagcatgtc ctccagcacc aggatttggc cgtcgcaggc
     2881 gacgctgccg ccgatgccga tggtcggaat cggcacatcg gcggtgatct tgcgggcgag
     2941 cggctcggcc accgcctcga tcaccaccga gaacgcgccg gcgtcgctga ccgcccgggc
     3001 atcgtcctcg atcggcgccc agtcggcctc gtcgcggccc tgcgcgcgaa agctgccgat
     3061 ggtgttgatt gactgcggcg tgagcccgat atggcccatc accgggacgc cacgctcggc
     3121 cagaaagcga atcgtctcgg ccatccgccg gccgccctcg agcttgatgg cgccgcaacc
     3181 ggtttccttc atcacccgcg ccgccgacat gaacgcctgc tccttcgacg cctcgtagga
     3241 gccgaacggc atgtcgatga cgaccagcgc ccgcttcgac ccgcgcatca ccgcgtggcc
     3301 ttgcaggatc atcatgtcga gggtgacggg gacggtcgat tcgagcccgt gcatcaccat
     3361 gccgagcgag tcgccgacca ggatgacgtc gcaatgtgcg tcgagcaggc gcgcggtgtg
     3421 ggcgtgatac gaggtcagcg agacgatcgg ctcgccgccc ttgcgggcgc ggatgtcagg
     3481 cgctgtcagc cgcttgattt cttcttgcac ggacatggtt gaacttggat ttgctgcggt
     3541 ctttcgattg tcatgcccgc gcttgtcgcg ggcacaactt acactgcctc acccaatctt
     3601 aatacgccgg gcggccgaat accggcacgc ccaccaccag cggatggaac acgaggccga
     3661 gcgccagata aagcagggtc ccgaccacga tggcgatgag gtcgttgcgc cggccccgca
     3721 ccgggatctc c
//
LOCUS       DASTZO010000180         4189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1137772,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000180 DASTZO010000000
VERSION     DASTZO010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137772"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(164..319)
                     /locus_tag="VFU20_05015"
     CDS             complement(164..319)
                     /locus_tag="VFU20_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285857.1"
                     /translation="MESDARYYRRRAIEERMAAQRAVTEQARTWHAKLARDFAERAAN
                     APNFVGA"
     gene            516..770
                     /locus_tag="VFU20_05020"
     CDS             516..770
                     /locus_tag="VFU20_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285858.1"
                     /translation="MLNSIDARRGQFDPAQRGYHAVYRDNEVNHCPGCGRTHWYLGRV
                     LAECAFCSTALPLQETFRHGPAPAPVIRRSRPDYAALDAA"
     gene            816..1160
                     /locus_tag="VFU20_05025"
     CDS             816..1160
                     /locus_tag="VFU20_05025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285859.1"
                     /translation="MPRFLFTLAAAFLMLAPAPAMAGAGSFIIVNSTGHDITALSIRR
                     FGTSNWQPLTGAPRAGGRGTIQFADADCAFDIQATLAGGITAVWSGVNLCEAKAVTLN
                     RIESGAVWVDYD"
     gene            complement(1504..2757)
                     /locus_tag="VFU20_05030"
     CDS             complement(1504..2757)
                     /locus_tag="VFU20_05030"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HEU5285860.1"
                     /translation="MALTEINFDGIIGPSHNYAGLSLGNLASTRNAGQVSRPRAAALE
                     GLAKMRSNLALGLAQGVLMPHPRPNRAWLAELGTTIDDAEAPLAANALSASAMWAANA
                     ATVSPAPDTADGRCHLTVANLRTMAHRSHEWPATLAQLKLAFADSAQFTVHPPVPPAF
                     GDEGAANHMRLAPTYGEPGVEIFVYGVSGGGFPARQHVEASKAIARRHRLDPGRTLFA
                     EQSEQAIAAGAFHNDVVAVANGPLLFAHEKAFAERARLLADCERLVPGFELVEVADAD
                     VPLADAVASYLFNAQLVSPAGGETTLIVPSEARETPSVWAWLQRHLAGNGPIRRVEVV
                     DVRQSMANGGGPACLRLRVVADPATVDPRFLADEARLDSIAETVERHWPEEIASDELA
                     EPSLIRDIEDARARLLQRLGLAGLL"
     gene            complement(2759..3775)
                     /locus_tag="VFU20_05035"
     CDS             complement(2759..3775)
                     /locus_tag="VFU20_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HEU5285861.1"
                     /translation="MTFRVRPARGSDFNAIYQMAKLTGGGFTNLPADRATLVEKLARS
                     DRSFARAGDEQAGDLYMFVLEDPKARQIRGTCQVFGKVGVVQPFYSYHLSTLTQSSPE
                     LGKTFHNQMLSLTTDLEGSSEVGGLFLHPQVRAGGWGSLLARSRYLFIRQHRSRFGDR
                     VLAELRGVMDEAGNSPFWDGLAGRFFDMSFPEADHFNAIHGTRFIADLMPRTPIYVAL
                     LAESAKAVMGQPHPSGRAALRMLEEEGFAYDRYIDIFDGGPTVTAATDRIRTVHESAC
                     EAVLEVGEGGKQKVILAAGKLSDFTACYASVRRVPRKGLCIDSEAARLLGVKPGDEVL
                     AVSS"
     gene            complement(3772..>4189)
                     /locus_tag="VFU20_05040"
     CDS             complement(3772..>4189)
                     /locus_tag="VFU20_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5285862.1"
                     /translation="PDLAVLRVNMRPRTPQIEAEAKRQIEEIVAVVAVERDVTIHAHG
                     DFGRPPKPMTAEMEKLFGLVKQAGADLGQQIGWQPSGGVCDGNNIAACGVPVVDTMGV
                     RGGKIHSPQEYLIVDSLAERAALSALTILRLAGGRA"
BASE COUNT          650 a         1381 c         1491 g          667 t
ORIGIN      
        1 cgaagcgctt gtcgctgccg gtgtcgacca attcacgagg catgattctc tcccgttttt
       61 caatgccctg cagaatcgac aaggctcggg agagttccaa cacctgtcct gtttcgcgcc
      121 gccggcgcgg caggcggaga attgcgggcg ttgaagcggg cgctcaggcg ccgacgaaat
      181 tgggggcgtt ggcggcgcgc tcggcgaaat cgcgggccag cttggcgtgc caggtccgcg
      241 cctgctcggt caccgcgcgc tgggcggcca tgcgctcctc gatggcccta cggcgataat
      301 agcgcgcgtc cgactccatc ttatgcccct cccgacaaaa gtcgctgggc cgacaatggc
      361 gcgtgattcg ctaagggagg gttaaaatgg gaagctgtgc cagcctgaca caaattgcgg
      421 cgattattgg caaattggcc ggttacatcg aagtccgccg caacttataa gaaataccgg
      481 acgttggagc cgaaatggtt tcaaggaagg tcactatgtt gaactcgatt gacgcccgcc
      541 gcggccagtt cgatccggcc cagcgcggct atcacgcggt ctaccgcgac aatgaggtca
      601 accactgccc cggctgcggc cggacgcact ggtacctcgg gcgagtcctt gcggaatgcg
      661 cgttctgctc gaccgcgctg ccgctccagg agacgttccg gcacggcccg gctccggcgc
      721 cggtgatccg gcgctcgcgc ccggactacg ccgcgctcga cgcagcctga cagtttcgct
      781 tggagcagca gtggcttgcg cggtataagc ggcgcatgcc ccggttcctg ttcacgcttg
      841 ccgcagcgtt cctgatgctg gcgcctgcac cggcaatggc cggggcgggc agcttcatca
      901 tcgtcaactc gaccggccat gacatcaccg ctctgtcgat ccggcggttc gggaccagca
      961 actggcaacc gctgaccggc gcgccgcgcg cgggcgggcg cgggacgatc cagttcgcgg
     1021 atgcggactg cgcgttcgat atccaggcga ccctggcggg cgggatcacc gcggtctggt
     1081 cgggcgtgaa cctgtgcgaa gcgaaagcgg taacgctgaa ccgaatcgag tcgggcgcag
     1141 tctgggtcga ctacgactga ccgacgggtg ccgctgcggc ggcgaataag ccccgacttt
     1201 cgcggccgcc ggggccggct tgacggagtc gagcaatttg gcgccggcca ggaacaccac
     1261 tccggtgcag gcgagcgcca gcatgatccc gccatagccc ggataggctt cgatatagtg
     1321 gcggccgcgc tcgacggcgc ccagagcgag cgcatagatt accagccaca cctcggtgcg
     1381 ggtcttgatc gtaaacagtc gggcgattcg catgagcatg gtgataagtt aatccaacca
     1441 cgtgccaacc gcgcagttcc gccagtcccg gactaagaca attggtcaat tgcccgttaa
     1501 cttttacagc agcccggcaa ggccgagccg ctgcagcagc cgggcgcgcg cgtcttcgat
     1561 gtcgcggatc agggacggct cggcgagctc gtcgctggcg atctcctccg gccagtggcg
     1621 ctcgacggtt tcggcgatcg aatcgagccg cgcctcgtcg gccaggaacc tcgggtcgac
     1681 ggtcgccgga tcggcgacca ccctgaggcg caggcaggcc gggccgccgc cgttggccat
     1741 cgactggcgg acgtcgacga cttcgacccg gcggatcggg ccgttgccgg cgagatgccg
     1801 ctgcagccac gcccacaccg acggagtctc gcgcgcttcg ctggggacga tcagggtcgt
     1861 ctcgccgccg gccgggctga ccagctgcgc gttgaacaga taggacgcga ccgcgtcggc
     1921 gagcgggacg tcggcgtcgg ccacctcgac cagctcgaag cccgggacca gccgctcgca
     1981 gtcggcaagg agccgggcgc gctcggcgaa cgctttttcg tgcgcgaaca ggagcggacc
     2041 gttggcgacc gcgacgacgt cgttgtggaa cgccccggcg gcgatcgcct gctccgactg
     2101 ctcggcgaac agggtccgcc cggggtccag ccggtgccgc cgggcgatcg ctttcgacgc
     2161 ctcgacgtgc tggcgcgcgg ggaagccgcc gcccgacacg ccgtagacga agatctccac
     2221 cccgggctcg ccgtaggtgg gggccaggcg catgtggttg gcggcgccct cgtcgccgaa
     2281 cgccggcggc accggtgggt ggacggtgaa ctgcgcggag tcggcgaagg cgagcttgag
     2341 ctgcgcgagg gtcgccggcc actcgtggct gcgatgggcc atggtgcgca aattggcgac
     2401 ggtcaggtgg cagcggccat cggcggtgtc gggcgccgga gagacggtcg ccgcgttggc
     2461 cgcccacatc gccgaggccg acagggcgtt ggcggcgagc ggggcttcgg cgtcgtcgat
     2521 ggtcgtcccg agctcggcga gccacgcccg gttgggccgc gggtggggca tcagcacgcc
     2581 ctgggcgagg ccgagggcga ggttggagcg catcttcgcc aacccttcga gcgcggcggc
     2641 gcgcggccgc gagacctgcc cggcgttgcg ggtcgaggca aggttgccga ggctgagccc
     2701 ggcgtaattg tggctcgggc cgatgatccc gtcgaaattg atttcggtga gcgccatctc
     2761 agctgctcac cgccagcacc tcgtcgcccg gcttgacccc gagcagccga gccgcctcgg
     2821 aatcgatgca caggcccttg cgcgggaccc gccggacgct cgcgtagcag gcggtgaagt
     2881 cgctcagctt gccagccgcc aggatcacct tctgcttgcc gccctcgccg acctcgagca
     2941 cggcctcgca ggcggattcg tgaacggtcc ggatgcggtc ggtggcggcg gtgacggtcg
     3001 ggccgccgtc gaagatgtcg atgtagcggt cgtaggcgaa gccttcttcc tcgagcatcc
     3061 gcaacgccgc ccgcccgctc gggtgcggtt ggcccatcac cgccttggcg ctctcggcga
     3121 gcagcgcgac gtagatcggg gtccgcggca tcaaatcggc gatgaagcgg gtgccgtgga
     3181 tggcgttgaa gtggtcggcc tcggggaagc tcatgtcgaa gaagcggccg gccagcccgt
     3241 cccagaacgg cgaattgccg gcctcgtcca tcactccgcg aagctcggcg agaacccggt
     3301 cgccgaagcg ggagcgatgc tgccggatga acagatagcg gctgcgggcg aggagcgagc
     3361 cccagccgcc ggcgcggacc tgggggtgga gaaacaggcc accgacctcg gacgaaccct
     3421 cgaggtcggt ggtcaggctg agcatctggt tgtggaaggt cttgccgagc tcggggctgc
     3481 tctgggtgag ggtgctgaga tgatagctgt agaacggctg gacgaccccg accttgccga
     3541 acacctggca ggtgccgcgg atctggcgcg ccttggggtc ctcgagcacg aacatgtaga
     3601 gatcgccggc ctgctcgtcg ccggccctgg cgaacgaccg gtcggaccgg gcgagctttt
     3661 cgaccagggt cgcgcggtcg gcgggcaggt tggtgaagcc gccgccggtc agcttggcca
     3721 tctggtagat cgcgttgaaa tcgctgccgc gagcgggccg gacccggaac gtcatgcccg
     3781 cccgcccgcc aggcgcagga tggtcagcgc cgacaaagcg gcgcgctcgg cgaggctgtc
     3841 gacgatgaga tattcttgcg ggctgtggat cttgccgccg cgaacgccca tcgtgtcgac
     3901 gaccggcacc ccgcaggcgg cgatgttgtt gccgtcgcac accccgcccg acggctgcca
     3961 gccgatttgc tggccgaggt cggcgccggc ctgcttgacc aggccgaaca gcttttccat
     4021 ctccgccgtc atcggcttgg ggggacggcc gaagtccccg tgggcgtgga tggtgacgtc
     4081 gcgttcgacc gccaccaccg cgacgatctc ctcgatctgg cgcttcgctt cggcctcgat
     4141 ttgcggcgtc cgcggcctca tgttcaccct caacaccgcg aggtcggga
//
LOCUS       DASTZO010000181         3523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1111927,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000181 DASTZO010000000
VERSION     DASTZO010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3523
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111927"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..504)
                     /locus_tag="VFU20_05045"
     CDS             complement(<1..504)
                     /locus_tag="VFU20_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5285863.1"
                     /translation="MARRLEQFPNLVTMFLTRAAEKGDQPFLSAKRDGEWRSLSWSEA
                     ARQVAALAESLTRIGLEPGDRVMLVSENRPEWLVADLAIMAAGCVTVPTYITNTTRDH
                     SHVLENSGAKAVIVSNQKLAKALVPAVLFSGLCRHVIGIEPIRAGQASDEFTYHHWDD
                     LVAGEPDM"
     gene            complement(512..2128)
                     /gene="ggt"
                     /locus_tag="VFU20_05050"
     CDS             complement(512..2128)
                     /gene="ggt"
                     /locus_tag="VFU20_05050"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU5285864.1"
                     /translation="MVSAADPRAAAAGAEMLRRGGSAVDAAIATLLALNVVEPQSSGI
                     GGGGFLVYSDRGGDPVTYDGRETAPAAAGPSWFLKDGRPMPFDEAVPGGKSVGVPGNV
                     RLMALAHGKHGKLPWAMVFEPAIRLARDGFAMSPRLNFALARGDDEGSSVAASTPAGR
                     ALYFDRDGRPLAVGTTIRNPAFAALLEQLAALGPDSFYVGPNAQAIVAAVNGSARNPS
                     PMTAGDVAAYEAKPRTPVCGHYRAHRVCGMGPVSSGGTTVFAILKQLERFDLAALGPN
                     HPTSWHLIAESMRLAFADRDKYLADPDFVRVPVAGLIDPGYLAGRSALIAADGTMAGV
                     AAGVPPGAPVAAVGEEAAEHGTSHFVAVDRWGNVASQTSTIESAFGSGLVVNGYYLNN
                     ELTDFSFVPAVAGVPVANRVEGGKRPRSSMSPTIVWGPDGRVRLALGAAGGPTIIAQV
                     AKAIIGVIDWKLSAQQAIALPTVFAPGDTVYLERGTFLEPMAPQLRALGHARIGYRPP
                     MTFKANAIEWAGGRWVGAADPRSEGAAVSE"
     gene            complement(2258..3289)
                     /locus_tag="VFU20_05055"
     CDS             complement(2258..3289)
                     /locus_tag="VFU20_05055"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HEU5285865.1"
                     /translation="MALYPLLRPLMFALDAERAHRATIAALKLWPKRRPPDFPPSLRS
                     TVAGLAFPSPVGLAAGFDKDAEVPAQMLSLGFGFVEVGTLTPEPQPGNSKPRLFRLRE
                     DRAVINRLGFNNRGQAEAFERLQTLGRRGVIGVNIGANRDSPDRIEDYVGGVRAMAQV
                     ARYLAINISSPNTPGLRGLQDEGALDELLAAVRDARPPGGPPLFLKVAPDLGPGDPER
                     IVRSAIDHGVDALIVANTTVSRPPLRSRHASESGGLSGEPLKPLALETLRRFRSAGGG
                     AIPLIGAGGLANADDAWERIRAGASLVQLYTAMVYEGPGIARRIARGLARKLERAGLA
                     NIAEAVGSG"
BASE COUNT          505 a         1207 c         1323 g          488 t
ORIGIN      
        1 catgtccggc tcgccggcga ccagatcgtc ccagtgatgg taggtgaatt cgtcgctcgc
       61 ctggccggcg cggatcggct cgatgccgat cacatggcgg cacaggcccg agaacagaac
      121 cgccgggacc agcgccttgg ccagcttctg gttcgacacg atcaccgctt tggcgcccga
      181 attctcgagc acgtggctgt gatcgcgggt ggtgttggtg atgtaggtcg gcaccgtgac
      241 gcagccggcg gccatgatgg cgaggtcggc gaccagccat tcggggcggt tctcgctgac
      301 cagcatcacc cggtcgccgg gctcaagccc gatgcgagtc aggctctcgg cgagcgcggc
      361 gacctggcgc gcggcctcgc tccagctcag cgagcgccac tcgccgtcgc gcttggccga
      421 caggaacggc tggtcgccct tctccgcggc gcgcgtgagg aacatcgtca ccagattggg
      481 gaattgctcg agccggcggg ccatcagcgc ctcattcgga aaccgccgcg ccttcgctgc
      541 gcgggtcggc ggccccgacc cagcggccgc cggcccattc gatggcgttg gccttgaagg
      601 tcatcggcgg ccggtagccg atcctggcat ggccgagcgc tcgaagctgc ggcgccatcg
      661 gctcgaggaa ggtcccgcgt tcgagataga cggtgtcccc gggcgcgaac acggtcggca
      721 aggcgatcgc ctgctgcgcc gatagcttcc agtcgatgac tccgatgatc gccttggcga
      781 cctgggcgat gatcgtcggc ccgccggcgg cgccaagcgc caggcggacc cggccgtccg
      841 gcccccagac gatggtcggg ctcatcgagc tgcgcgggcg cttgccgccc tcgactcggt
      901 tggcgacggg gactccggcg accgcgggaa cgaagctgaa atcggtcagc tcgttgttga
      961 gataatagcc attgaccacc aggcccgagc cgaacgcgct ttcgatcgtc gacgtttgcg
     1021 aggcgacgtt gccccagcgg tcgaccgcga caaagtgcga cgtcccgtgc tccgctgctt
     1081 cctcgccgac ggcggcgacc ggcgctcccg ggggcacgcc cgcggcgacg ccggccatag
     1141 tgccgtcggc ggcgatcagg gcggagcggc cggcgagata gccgggatcg atcagcccgg
     1201 cgaccggcac ccggacaaaa tcgggatcgg cgagatattt gtcgcggtcg gcgaaagcca
     1261 gccgcatcga ttcggcgatc agatgccagc tggtcggatg gttcgggccg agcgccgcca
     1321 ggtcgaagcg ctcgagctgc ttgaggatcg cgaacaccgt ggtgccgccc gacgacaccg
     1381 ggcccatgcc gcagacgcgg tgggcgcgat agtggccgca caccggggtg cggggcttgg
     1441 cttcatatgc ggcgacatcg cccgctgtca tcggcgaggg attgcgcgcg ctgccgttga
     1501 ccgccgcgac gatcgcttgc gcgttcgggc cgacgtagaa gctgtccggc ccgagcgcgg
     1561 ccaactgctc gagcaaagcg gcgaaggcgg ggttgcggat ggtcgtgccg acggccagcg
     1621 ggcggccgtc ccggtcgaaa tagagggctc gcccggcggg cgtcgaggcc gccacgctgc
     1681 tgccttcgtc atcgccgcgg gcgagcgcga aattgagccg cgggctcatc gcgaacccgt
     1741 cgcgggcaag acggatcgcg ggctcgaaca ccatcgccca gggaagcttg ccgtgcttgc
     1801 cgtgggccag agccatcagc ctcacattgc cgggcacgcc gacgcttttg ccgccgggga
     1861 ccgcctcgtc gaacggcatc ggccggccgt ccttcaggaa ccacgacggc cccgccgcgg
     1921 ccggcgccgt ttcgcggccg tcgtaggtga ccgggtcgcc gccgcgatcc gaatagacga
     1981 ggaacccgcc gccgccgatg cccgagcttt gcggctcgac gacattgagc gccagcagcg
     2041 tcgcgatcgc cgcatcgacg gcgctgccgc cccgccggag catttcggcc ccggccgcgg
     2101 cggcgcgcgg gtcggcggcg ctgaccattc ctgtcccgat cgaagccgag cgggcaggcg
     2161 ggccagccga agggggggga agggccgcgc agccggtgag ggcgagggcg gcgaggacgg
     2221 cgaagacggg cgtgacgagc gttggcatcg gcctcggcta gccgctcccc accgcctcgg
     2281 caatgttcgc gaggccggcg cgctcgagct tgcgggcgag gccgcgggcg atccgccggg
     2341 cgatgcccgg gccctcgtag accatcgccg tgtagagctg gacaaggctg gcgccggcgc
     2401 ggatccgctc ccaggcgtcg tcggcgttgg cgaggccgcc ggcgccgatc agggggatgg
     2461 cgccgccgcc cgcgctccgg aaccggcgca gggtttcgag cgccagcggc ttgagcggct
     2521 cgccggacag gccgccggat tcgcttgcgt ggcgcgagcg caagggcggg cgcgagacgg
     2581 tggtgttggc gacgatcagg gcgtcgactc cgtggtcgat cgccgagcgg acgatgcgct
     2641 cggggtcacc ggggccgagg tcgggcgcga ccttgaggaa cagaggcggg ccgcccggcg
     2701 ggcgggcgtc gcggacggcg gcgagcaatt cgtcgagcgc gccttcgtcc tgcaggccgc
     2761 gcaggccggg cgtgttgggc gaggagatgt tgatcgccag gtagcgcgcg acctgggcca
     2821 tcgcccgaac gccgccgaca tagtcttcga ttcggtcggg actgtcgcgg ttggcgccga
     2881 tgttgacccc gatgacgccg cggcggccca gggtttggag ccgttcgaac gcttccgcct
     2941 ggccgcggtt gttgaagccc aggcggttga tcaccgcgcg gtcctcgcgc agccggaaca
     3001 ggcggggctt cgaattgccg ggctgcggct ccggggtcag ggtcccgacc tcgacgaagc
     3061 cgaagccgag gctgagcatc tgcgcgggca cctcggcgtc cttgtcgaag ccggcggcga
     3121 gcccgaccgg cgaggggaac gccagcccgg cgacggtcga gcggagcgac ggcgggaaat
     3181 cgggcggccg gcgcttcggc cacagcttca gggcggcgat cgtcgcccgg tgcgcgcgct
     3241 ccgcgtcgag cgcgaacatc agcggccgga gaagcgggta gagcgccatc aagtcccgtc
     3301 gttggtcgaa cagtcgtcac cgcggccttg agccggggcc ggcctttgtt caaggagaag
     3361 cgcatgacga aaagaggaaa tcccgtgaag cgcttactcc tgctcgcctc gacggccctc
     3421 attgccgcct gcgccgcagc tcccccgccg ctcgccgtgc cgccggccga gcaagcggcc
     3481 gccgcggtgc cggcggtccc ggccaagaac gccgagctcg ccg
//
LOCUS       DASTZO010000182         2662 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_119416,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000182 DASTZO010000000
VERSION     DASTZO010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2662)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2662)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2662
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119416"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            203..>2662
                     /locus_tag="VFU20_05060"
     CDS             203..>2662
                     /locus_tag="VFU20_05060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010238989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5285866.1"
                     /translation="MIRSRLIVLLASASALAACATAPRQVVLAPQPAPVAELTVAPAP
                     APVPALVEQVAIPHQMFRLANGLTVLVHEDRKAPVVAVSTWYNVGSKDEPEGKTGFAH
                     LFEHLMFNGSENLPGDYFEYLQQIGATDYNGTTHFDRTNYFQTVPTGALERALFMESD
                     RMGYLLGAVTQAKLDNQRGVVQNEKRQNDNQPGGLVFYEILNNLFPEGHPYRHSPIGS
                     MADLDAASLADVKSWFRDNYGPNNAVLVLAGDVDADSARPLVEKYFGEIGQGPVNVPA
                     DAGVPVLAAPKSIVMKDRVAAVSVQRYWPMPGILDRQLVALDIAGSVLGGLASSRLDN
                     ILVRDEKVAVGVTAGNIPFQRVGIFTATATVKPGVDPALVERRLDELITQFIAQGPSD
                     DEVRRAATSEVAQRIRGLEQVGGFGGKAVALAEGQIYAGDSEFYRKTLATYASITPDE
                     VRAAMNEWLTRPSLTIRLEPGDRPAYQEAKAVALAGKKGDGKVTVSRKREVPALGPMA
                     PLDFPEVSHVTLANGVKVHYAQRTAVPVTGLALSFNAGYSADAPDSRGLQNLTVGLLD
                     EGTTTRSSQQIAEEQERLGAQIGAGGSADRSTVGMSALSANLGPSLDLLADIVRNPAF
                     AEPELNRVRAQTLTAIAQQLKDPNGMASRALPALLYGPAHPYATTAAGDPAAVARFTR
                     ADLVGFQQRWLRPDNLQIFVVSDRPLAEIQAELDRRFGQWAAPDVPRGTKQFGAAPAR
                     PESPKIFLIDRPQSPQSVILGGQVTPVDPRSDVTGLTSANDVIGGNFLARINMNLRET
                     KGWSYGVRGTVQLNAKVVPYVV"
BASE COUNT          438 a          974 c          884 g          366 t
ORIGIN      
        1 atcgagaaat tgcggatctt gtcgagggga gtcatgcgcg ggcgggctag cagagcggcc
       61 cgggcctgtc agcacgcgcc gccggaaccg tcgtcgcgac cgccgttggt cgaaccggcg
      121 ggcactctgg cagaaccggc ttgcgctgga tggaaccaac cctacacatg gcgcgcaccc
      181 ccgcatcccg gagcaaaagt cgatgattcg cagccgcttg attgtcctgc ttgcaagcgc
      241 gtcggcgctc gccgcctgcg cgaccgcacc gcgccaggtc gtgctcgcgc cgcagccggc
      301 gccggtcgcc gagctcacgg ttgcgcccgc cccggctccg gtcccggcgc tggtcgagca
      361 ggtcgccatc ccgcaccaga tgttccgcct cgccaacggc ctcaccgtgc tggtccacga
      421 ggaccgcaag gcgccggtgg tcgcggtcag cacctggtac aatgtcggct cgaaggacga
      481 gcccgagggc aagaccggct ttgcccatct gttcgagcat ctgatgttca acggctcgga
      541 gaatctgccc ggcgattatt tcgaatatct gcagcagatc ggcgccaccg actacaacgg
      601 caccactcac ttcgaccgca ccaattattt ccagaccgtg ccgaccggag cgctcgagcg
      661 ggcgctgttc atggagtccg accgcatggg gtacctgctc ggcgcggtca cccaagccaa
      721 gctcgataac cagcgcggag tcgtccagaa cgaaaagcgc cagaacgaca accagccggg
      781 cggcctggtg ttctacgaga tcctcaacaa cctgttcccc gaaggccacc cgtaccggca
      841 cagcccgatc gggtcgatgg ccgacctcga cgcggccagc ctcgccgacg tgaaaagctg
      901 gttccgcgac aattacgggc ccaacaatgc ggtgctggtg ctggccggcg acgtggacgc
      961 cgactccgcc cggccgctgg tcgagaaata tttcggcgag atcggccagg gcccggtcaa
     1021 cgtccccgcg gacgccggcg tcccggtgct cgccgcgccc aagtcgatcg tcatgaagga
     1081 ccgggtcgcc gcggtcagcg tccagcgcta ctggccgatg cccggaatcc tcgaccggca
     1141 gctggtcgcg ctcgacatcg ccggctcggt gctcggcggc ctcgccagct cgcgcctcga
     1201 caatatcctg gtccgcgacg agaaggtcgc ggtcggggtc accgcgggca acatcccgtt
     1261 ccagcgcgtc ggcatcttca ccgccaccgc gacggtcaag ccgggggtcg acccggcgct
     1321 ggtcgaacgg cgcctcgacg agctgatcac ccagttcatc gcgcagggcc ccagcgacga
     1381 cgaggtccgg cgggccgcga ccagcgaggt cgcgcagcgc atccgcggcc tcgagcaggt
     1441 cggcggcttc ggcggcaagg cggtcgcgct cgccgaaggc cagatctacg ccggcgattc
     1501 cgaattctac cgcaagacgc tcgcgaccta cgcgagcatc acgcccgacg aggtgcgcgc
     1561 ggcgatgaac gagtggctga ccagaccgtc gctgaccatc cggctcgagc ccggcgaccg
     1621 gccggcctac caggaggcga aggcggtcgc tttggccggc aagaagggcg acggcaaggt
     1681 cacggtctcc aggaagcgcg aggtcccggc gctcgggccg atggcgccgc tcgacttccc
     1741 cgaggtcagc cacgtgaccc tggcgaacgg ggtcaaggtc cactacgcgc agcgcaccgc
     1801 ggtcccggtg accggcctcg ctctgtcgtt caacgccggc tattccgccg acgcgcccga
     1861 cagccgcggc ctgcagaatt tgaccgtcgg cctgctcgac gaaggcacga caacgcgaag
     1921 ctcgcagcag atcgcggaag agcaggagcg actgggcgcc cagatcggcg ccggcggatc
     1981 ggccgaccgg agcacggtcg ggatgtcggc gctgagcgcc aacctcggcc cgtcgctcga
     2041 cctgctcgcc gacatcgtcc gcaacccggc cttcgccgag cccgagctca accgggtgcg
     2101 ggcgcagacc ctgaccgcga tcgcccagca gctcaaggac cccaacggga tggcgagccg
     2161 ggccttgccg gcgctgctct acggtccggc gcatccctat gcgacgaccg ccgcgggcga
     2221 cccggcggcg gtggcgcgct tcacccgcgc cgacctggtc ggcttccagc agcgctggct
     2281 gcggcccgac aatctgcaga tcttcgtcgt ctccgaccgg ccgctggccg agatccaggc
     2341 ggagctcgac cggcgcttcg gccagtgggc ggcgcccgac gtcccgcgcg gcaccaagca
     2401 gttcggcgcc gccccggcgc ggccggagag cccgaagatc tttctcatcg accgcccgca
     2461 atcgccgcag tcggtgatcc tcggcggcca ggtgaccccg gtcgacccgc gcagcgacgt
     2521 caccggcctg accagcgcca acgacgtcat cggcggcaac ttcctcgccc gcatcaacat
     2581 gaacctgcgc gagaccaagg gctggtccta cggcgtgcgc gggacggtgc agctcaatgc
     2641 caaggtcgtg ccctatgtgg tg
//
LOCUS       DASTZO010000183         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_616040,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000183 DASTZO010000000
VERSION     DASTZO010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616040"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..1834
                     /locus_tag="VFU20_05065"
     CDS             200..1834
                     /locus_tag="VFU20_05065"
                     /inference="COORDINATES: protein motif:HMM:NF018875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HEU5285867.1"
                     /translation="MIRVIIYLVLIGLLAFVAVWFADRPGDVSVTWQGWRLETSVMML
                     LVAMAAVAVVAVLLWSLALTVWRSPDTLARYLGARRGIRGYRAVSQGLIAIGSGDVRA
                     ARKYVDEANRHAPGEPLTLLLGAQTAQLAGDGAQAERAFHQMANRDDTRLLGLHGLYI
                     EAQRRADVATAQLYAEEAAKHAQVPAWAGQAVLEFRCVTGDWAGALERLERNMKSGLI
                     DKPAYQRQRAVLLTARALANEEMERDRAKADVLEAVKLAPTLVPAAVLAAKLLGEGGE
                     HRKAGRIIEAAWRTNPHPDLAEGYAHLRPGDSARERLTRIQSLAEKSPGNVEAALAVG
                     RAALDAQEFAIARQALAPLAVVPTQRVAALMAELEMKEHNDEGRAREWMARALHGRRD
                     PAWTADGFVSQRWLPISPVTGRLDAFEWKDPLSGLDSDRPVIEAGRDADATVNVPAKV
                     ERRPAHETPLDLTAEKPAPLRAEEPRPEPIIEPPPVTSTRAARSEPPVSRPPAVIPLV
                     HAPDDPGPDLEATVEPVPASEAKPDVWGRLRQYFKT"
     gene            1954..2029
                     /locus_tag="VFU20_05070"
     tRNA            1954..2029
                     /locus_tag="VFU20_05070"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1987..1989,aa:Thr,seq:cgt)
BASE COUNT          310 a          737 c          714 g          286 t
ORIGIN      
        1 cgcacattcc gatctcgccg gcgcactcgc ggagctcgcc aagctgcccg acaatgtgcg
       61 tgccccggcg caggactgga tcaagcgcgc cgaagcgcgt acggctgcga tccggtcgag
      121 caatcaaatc gcggccgagg ccctggccgc gcttgggaag tgacttgatt gggcatccgc
      181 caatggcagt aaggtcgcga tgatccgcgt catcatctac ctcgtcctca tcggcctcct
      241 ggccttcgtc gcggtctggt tcgccgaccg gccgggcgac gtctcggtga cgtggcaggg
      301 ctggcggctc gaaacctcgg tgatgatgct gctggtcgcg atggcggccg tggcggtggt
      361 ggcggtgctg ctgtggtcgc tggcgctcac cgtctggcgg tcgcccgaca cgctcgcccg
      421 ctacctcggt gcgcggcgcg gcattcgcgg ataccgggcg gtgtcccagg ggctgatcgc
      481 gatcggctcg ggtgacgttc gcgccgcccg caagtacgtc gacgaggcca accgccatgc
      541 gccgggcgag ccgctgacgc tgctgctcgg cgcgcagacc gcgcagctcg cgggcgacgg
      601 cgcccaggcc gagcgcgctt tccaccagat ggcgaaccgc gacgacacca ggctgctcgg
      661 cctgcacggc ctctacatcg aggcgcagcg ccgcgccgac gtcgcgaccg cgcagctcta
      721 cgccgaagaa gccgccaagc acgcgcaggt tccggcctgg gccggccagg ccgtgctgga
      781 attccgatgc gtgaccggcg actgggcggg cgcgctcgag cggctcgagc gcaacatgaa
      841 gagcggcttg atcgacaagc cggcctatca gcgccagcgc gccgtgctcc tgaccgcgcg
      901 ggcgctcgcc aatgaggaga tggagcgcga ccgcgccaag gccgacgtcc tggaggcggt
      961 aaagctcgcc ccgaccctgg tcccggcggc cgtgctcgcc gcaaagctgc tcggcgaggg
     1021 cggcgaacat cgcaaggccg gccgcatcat cgaggccgct tggcggacca atccgcatcc
     1081 cgatctggcg gagggctacg cccatctgcg gcccggcgat tcggcccgcg aacggctcac
     1141 gcgcatccag tcgctggccg aaaaatcgcc cggcaatgtc gaggccgcgc tggccgtcgg
     1201 ccgcgccgct ctcgacgcgc aggagttcgc catcgcccgc caggcgctgg cgccgctcgc
     1261 cgtggtgccg acgcagcgcg tcgcagccct gatggccgaa ctcgaaatga aggagcataa
     1321 cgacgagggc cgcgcccgcg agtggatggc ccgcgcgctg catggccggc gcgatccggc
     1381 ctggacggcg gacgggttcg tctcgcagcg ctggctgccg atctcgccgg tcaccggccg
     1441 gctcgacgcg ttcgagtgga aggatccgct gtcgggtctc gacagcgacc gcccggtgat
     1501 cgaggccggc cgcgatgccg acgccaccgt caatgtcccg gccaaggtcg agcgccgccc
     1561 ggcgcatgag acgccgctcg atctcacggc cgagaagccg gcgccgctgc gcgccgagga
     1621 gccccggccg gagccgatca tcgaaccgcc gcccgtcacc agcacgcgcg ccgcacgatc
     1681 tgaaccaccg gtttcgcgcc cgccggccgt gatcccgctg gtgcacgcgc cggacgatcc
     1741 cggccccgat ctcgaagcga cggtcgagcc ggtaccggcg agcgaggcca agcccgacgt
     1801 ctggggccgc ctgcggcagt atttcaagac ttgaagcgga cgcctatttc ggggccggtc
     1861 caggccactc ggctgacccc cgcgtgttcg accgcgcctt gccaagccct gccacagccg
     1921 atatcagggc gcattcgcga tcaggcagat tgggccgcaa tagctcagcc ggtagagcac
     1981 gtcattcgta atgacggggt caggggttcg aatccctttt gcggcaccaa ccataaggcc
     2041 cggatat
//
LOCUS       DASTZO010000184         5391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1112555,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000184 DASTZO010000000
VERSION     DASTZO010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112555"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..957)
                     /locus_tag="VFU20_05075"
     CDS             complement(139..957)
                     /locus_tag="VFU20_05075"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEU5285868.1"
                     /translation="MVSHSGLITVMQRAARKAAPRLRRDFGEVEQLQVSRKGPADFVS
                     MADKRAEATIVEELRSARPDWGMLLEEGGEIEGNPDKPRWIVDPLDGTTNFLHGVPHF
                     AISIAVEERRPGSRPEITQGLVYQPLTDESFWAEKGRGAWLNERRLRVSARRDLSETL
                     IGTGIPHCGRGEVGSWGRIYAAVAPHVSGVRRFGSAALDLAWVAAARMDGFWEDDLDI
                     WDTAAGVLLIREAGGFVTDYRGSDQMFERRQYLAANGDIHSRLHKLVSGALRAI"
     gene            complement(961..1524)
                     /gene="efp"
                     /locus_tag="VFU20_05080"
     CDS             complement(961..1524)
                     /gene="efp"
                     /locus_tag="VFU20_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU5285869.1"
                     /translation="MKISGVDIRPGNIIEYDGGIWRAVKIQHTQPGKGGAYMQVEMKN
                     LIDGRKTNVRFRSAETVERVRLDTKDFQFLYHDGDMLVFMDKDTYEQVSLPEDLIGEA
                     AAFLQDGMDVVMELYNERPISVQLPDQIEATVVEADAVVKGQTASSSYKPAMLDNGVR
                     VMVPPHITAGTRIVVDVYEQTYVRRAD"
     gene            complement(1646..2281)
                     /locus_tag="VFU20_05085"
     CDS             complement(1646..2281)
                     /locus_tag="VFU20_05085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5285870.1"
                     /translation="MLKVVGTAVVTAILTSAFWIFYFGIAGAPRVSEPKVTAAGEKIT
                     IDPEAQPPVTIAEGVTVGPAGLAIPVAGIKPGQLSDTYTQARAGGARLHDAIDIMAPE
                     GTPVLAAADGTVEKLFFSQGGGGITAYVRSPDQRWIYYYAHLQGYAPGLAEGQRVKRG
                     QIIGRVGSTGNANPAGPHLHFAIHRMQPGEKWHQGSAVNPFPLLAGRKASS"
     gene            complement(2281..3354)
                     /locus_tag="VFU20_05090"
     CDS             complement(2281..3354)
                     /locus_tag="VFU20_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU5285871.1"
                     /translation="MAIGLFAAATAAVAMAQAAQAPAQQPASTTARGAKPWVPPGTPG
                     SPIDGELFHAQVLMSAHAFSPGVIDGKKGESFRLALSSFQRSRGLDATGELDRETRRA
                     LLQANRHSTVMVRLGPDDVRSQYVYPFPANPEKQAELKFLGYRNMLEKVAERYHTTPD
                     TIVALNGPDKLIGLGQVLRLPNVIPSARDYSGVQGNGASVMNVLNVDARQPQGDYVVV
                     DKSEGVLKVYRGSAPGEGADGDGAQLVAAFPVTMGSKQYPLPIGRWKATTYAFLPPFK
                     YQPELLVKPKTDKELNLPPGPNGPVGVAWLDLTKEHYGIHGTNEPQTIGRAESSGCIR
                     MTNWDVLRLARMMKPGFTAIFQA"
     gene            complement(3468..4154)
                     /gene="thiE"
                     /locus_tag="VFU20_05095"
     CDS             complement(3468..4154)
                     /gene="thiE"
                     /locus_tag="VFU20_05095"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU5285872.1"
                     /translation="MNEDPEDLAILPPPVRTEPCKLYLISVQDVGGAFPDRLRAALDA
                     GPVAAFQLRVKDRDEHELARLAEPLQRICADRGTAFIVNDSMSLAKRIGADGVHLGQS
                     DGDVREARALLGPAAQIGRTCHDSRHLAMEAGEAGADYVAFGAFYPTTTKPSNYRPHP
                     SILSWWSALFEIPCVAIGGITPDNARPLIDAGADFVAVCQAVWGASDPGEPVRRFTEL
                     FAATLADQLP"
     gene            4190..4558
                     /locus_tag="VFU20_05100"
     CDS             4190..4558
                     /locus_tag="VFU20_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285873.1"
                     /translation="MIRAAPFLALLALASCATVPHRDYGNGPTAPLNEVANLDGITVR
                     PLAVMEDSRCPASVQCVWAGRVRIRAEVSGEGIRELTLGEAVAVSGGTLTLVDVRPGK
                     RTPETIALRAYQFTFRFQQP"
     gene            complement(4555..>5391)
                     /locus_tag="VFU20_05105"
     CDS             complement(4555..>5391)
                     /locus_tag="VFU20_05105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HEU5285874.1"
                     /translation="QSGGSTPKALRLYGIEESEYSGEAEMFDLIHAMRVRIITSPAFG
                     GDKVIGAILFERTMDGDAKGRPVPDALWERGVVPFLKVDKGLEDVADGVRLMKPNPGL
                     DELLDRAKAKRVFGTKMRSVIELANRKGIADIVLQQFDTAERIARKDLMPIIEPEVSI
                     RSPERDAADRILLDELRQALDSLPDGRQVMLKLSIPAEPGLFAPLVRHPRVLRVVALS
                     GGFSRDEACRELARNEGMVASFSRALLEGLRHDLSDEEFDRTLARSIDQIHRASTAKV
                     PA"
BASE COUNT          815 a         1820 c         1864 g          892 t
ORIGIN      
        1 acggcgagga acagcaggat cggcagatat ccggcggcga cgctggccaa gggaaactcc
       61 actacgcgag gctggcgggg ctttaggaca gggggcgcgg gcgggcaaca ccgcgccgaa
      121 ggcaaatggc ggggaaaatc agatcgcccg cagggccccg gagaccagct tgtgcagccg
      181 tgagtggatg tcgccgttgg cggcgaggta ctggcgccgc tcgaacatct ggtcggagcc
      241 gcggtagtcg gtgacgaagc cgcccgcctc gcggatcagg agcacgcccg cggcggtgtc
      301 ccagatgtcg agatcgtcct cccagaagcc gtccatccgc gccgccgcga cccaggcgag
      361 gtcgagcgcc gccgagccga agcggcggac ccccgagacg tgcggagcga ccgccgcgta
      421 gatccgtccc cacgatccga cctcgccgcg cccgcaatgc gggatcccgg tgccgatcag
      481 ggtctcggac aggtcgcgtc gcgccgagac ccgcagccgc cgctcattga gccaggcgcc
      541 gcggcccttc tcggcccaga agctctcgtc ggtcagcggc tggtagacca ggccctgggt
      601 gatttcgggg cgcgagccgg gccgccgctc ctcgaccgcg atgctgatcg cgaaatgcgg
      661 caccccgtgg aggaagttgg tggtgccgtc gagcgggtcg acgatccagc gcggcttgtc
      721 gggattgccc tcgatctccc cgccctcctc gagcagcatt ccccaatcag gccgggcgga
      781 gcgcagctcc tcgacgatgg tcgcttccgc gcgcttgtcg gccatcgaga cgaagtccgc
      841 cggccccttg cggctgacct gcaattgctc gacctcgccg aagtcgcggc gcagccgcgg
      901 cgccgccttg cgcgcggccc gctgcatcac cgtgatcagg cccgagtggg agaccatcga
      961 tcagtccgcg cggcggacgt aggtttgttc atagacatcg accacgatcc gggtgccggc
     1021 ggtgatgtgc ggcggcacca tcaccctcac gccattgtcg agcatcgccg gcttgtagga
     1081 ggacgaagcg gtctggccct tcaccaccgc gtcggcctcg accaccgtcg cctcgatctg
     1141 gtcgggcagc tgaacgctga tcggccgctc gttgtagagc tccatcacca cgtccatccc
     1201 gtcctgcaaa aacgcagcgg cctcgccgat caagtcctcg ggcagcgaca cctgctcgta
     1261 ggtgtccttg tccatgaaca ccagcatgtc gccgtcgtgg tagaggaact gaaagtcctt
     1321 ggtgtcgagg cggacccgct cgaccgtctc ggcgctgcgg aagcggacgt tggtcttgcg
     1381 gccgtcgatg aggttcttca tctcgacctg catgtaggcg ccgcccttgc ccggctgggt
     1441 gtgctggatc ttcaccgcgc gccagatgcc gccgtcatat tcgatgatgt tgccgggacg
     1501 gatgtccacg ccgctgatct tcatgttctt ggcatcccat cagccgttcg ccctgagctt
     1561 gtcgaagggc tgtcttgttc ttggcgaaag gacagggctt cgacaggctc agcccgaacg
     1621 ggtgtttgtc gctatcgctg cgcccttaac tgctggcctt gcgaccggca agcagcggga
     1681 acgggttgac cgcgctgccc tggtgccatt tctccccggg ctgcatgcgg tggatcgcga
     1741 agtgcaggtg cgggccggcc gggttggcgt tgccggtcga cccgacgcgg ccgatgatct
     1801 gaccgcgctt gacccgctga ccctcggcga ggccgggcgc atagccctgc aggtgcgcgt
     1861 aataatagat ccagcgctgg tcgggcgagc ggacgtaggc ggtgatcccg ccgccgccct
     1921 gagagaagaa cagcttctcg accgttccgt cggccgcggc gagcacggga gtcccttccg
     1981 gggccatgat gtcgatcgcg tcgtgcagcc gggcgccgcc ggctcgcgcc tgggtgtagg
     2041 tgtcgctcaa ttgcccgggc ttgatccccg caaccgggat ggcgaggccc gccggcccga
     2101 ccgtcacccc ctcggcgatg gtcaccggcg gctgcgcctc ggggtcgatc gttatcttct
     2161 cgccagctgc cgtcaccttg ggctcgctta ccctcggagc gccggcaatt ccgaaataga
     2221 agatccagaa ggcgctggtc aggatcgccg tgacgaccgc ggtgccgacg accttgagca
     2281 tcaggcctgg aagatcgccg tgaagcccgg cttcatcatc cgcgcaagcc gcagcacgtc
     2341 ccaattggtc atccggatgc agccgctcga ttcggcccgg ccgatggtct gcggctcgtt
     2401 ggtgccgtgg atcccgtaat gctccttggt gaggtcgagc caggcgactc cgaccggccc
     2461 gttgggcccc ggaggcaagt tcagctcctt gtcggtcttg ggcttcacga gcagttcggg
     2521 ctggtacttg aacggcggca ggaacgcgta ggtagtcgcc ttccagcggc cgatcggcag
     2581 cggatattgc ttcgagccca tggtcaccgg aaaggcggcg accagctgcg cgccgtcccc
     2641 gtccgcgcct tcgcccggcg cgcttccgcg atagaccttg agcacgccct cgctcttgtc
     2701 gacgacgaca tagtcgccct gcggctggcg ggcgtcgacg ttgagcacgt tcatcacaga
     2761 ggcgccattg ccctggacgc ccgaatagtc acgcgccgag ggtatgacat tgggcagccg
     2821 gagcacctgg ccgaggccga tcagcttgtc cggcccgttg agcgcgacga tcgtgtcggg
     2881 cgtcgtgtga tagcgctcgg cgaccttctc gagcatgttg cggtagccga ggaacttcag
     2941 ctcggcctgc ttctccgggt tcgctgggaa cggatagaca tattggctgc gcacgtcgtc
     3001 cggcccgagg cggaccatga ccgtcgagtg ccggttggcc tgcagcaagg cgcgccgcgt
     3061 ctcgcggtcg agctcaccgg tggcgtcgag cccgcgcgag cgctggaagc tgctcagcgc
     3121 cagccgaaac gattccccct tcttgccgtc gatcacgccg ggcgaaaagg catgcgccga
     3181 catcagcacc tgagcgtgga acagctcgcc gtcgatcggc gagcccggag tgcccggcgg
     3241 aacccacggc ttggcgccgc gagcggtcgt tgatgcaggc tgctgggcgg gcgcttgggc
     3301 ggcctgggcc atggcgaccg cggcggtggc ggccgcgaac aggccgatgg ccaaatggga
     3361 tgtccttgga aacacgcaac tctcctgacg caatggggtt tgcgactgaa cgatggagcc
     3421 gggactagtt tccatgaccg ccgcgcaagg cgcgttccgc aggggcccta aggcagctgg
     3481 tcggccagag tggcggcgaa caattcggtg aatcggcgga ccggctcgcc cgggtctgag
     3541 gcgccccaca ccgcctggca gacggcgacg aagtcggcgc cggcgtcgat cagcggccgg
     3601 gcattgtcgg gggtgatgcc gccgatcgcg acgcacggga tttcgaacag cgccgaccac
     3661 cagctgagga tcgacgggtg cgggcggtag ttcgagggct tggtggtggt cgggtagaag
     3721 gcgccgaacg cgacatagtc ggcgcccgcc tcgcccgctt ccatcgccag gtgccggctg
     3781 tcgtggcagg ttcggccgat ctgggcggcc ggcccgagca gcgcgcgcgc ctcgcgcacg
     3841 tcgccgtccg actggccgag gtggaccccg tcggcgccga tcctcttggc gaggctcatg
     3901 ctgtcgttga cgatgaacgc ggtgccgcgg tcggcgcaga tgcgctgcag cggctcggcg
     3961 agccgcgcga gctcgtgctc gtccctgtcc ttgacgcgca gctggaacgc ggcgaccggg
     4021 ccggcatcga gcgccgccct gagccgatcc gggaacgcgc cgccgacgtc ctggacgctg
     4081 atcagataga gcttgcacgg ctcggttcga acgggcggcg gcaggatcgc caggtcctcg
     4141 gggtcttcgt tcatggcgcg ctcattagcg aatgagctag gacgggccaa tgatccgagc
     4201 agcccctttc ctggcccttc tggcgctcgc gtcctgtgcg acggtgccgc acagagatta
     4261 tggcaacggc ccgaccgccc cgctgaatga agtcgccaat ctcgacggca tcaccgtccg
     4321 gccgctggcc gtgatggagg acagccgctg cccggcctcg gtccaatgcg tgtgggcggg
     4381 ccgcgtgcgg attcgcgcgg aagtcagcgg cgagggaatt cgcgaactga cgctcggaga
     4441 agcggtggcg gtgagtggcg gcacactgac cctggtcgac gttcggccag gcaagcgcac
     4501 gccggaaacg atcgcgctgc gggcgtacca gttcaccttc cggtttcagc agccctaggc
     4561 cggaaccttc gccgttgagg cccggtggat ctggtcgatc gagcgggcga gcgtccggtc
     4621 gaactcctcg tcgctcaagt cgtgccgcag gccttcgagc agcgccctcg agaagctcgc
     4681 gaccatgccc tcgttgcggg cgagttcgcg gcacgcctcg tcgcgcgaga acccgcccga
     4741 caaagcgacg actcgcagca cccgcgggtg gcggaccaag ggcgcgaaca gcccgggctc
     4801 ggcggggatg gacagcttga gcatcacctg gcggccgtcg ggcaaagaat cgagcgcctg
     4861 gcggagctcg tcgagcagga tccggtcggc ggcatcgcgc tcggggctcc tgatcgacac
     4921 ctcgggctcg atgatcggca tcagatcctt gcgcgcgatc cgctcggcgg tgtcgaactg
     4981 ctgcaggacg atgtcggcga tgcccttccg gttcgcgagc tcgatcaccg agcgcatctt
     5041 ggtgccgaac acgcgcttgg ctttggcgcg gtcgagcaat tcgtcgagcc ccgggttggg
     5101 cttcatcagc cgcaccccgt cggccacgtc ctcgagcccc ttgtccacct tcaggaacgg
     5161 caccacgccg cgctcccaca gcgcgtccgg gaccggcctg cccttcgcgt cgccgtccat
     5221 ggtccgctcg aacaggatcg ccccgatcac cttgtcgccg ccgaacgccg gcgaggtgat
     5281 gatccgcacc cgcatcgcgt ggatgaggtc gaacatctcc gcctcgccgg agtactcgga
     5341 ctcctcgatc ccgtagaggc gcagggcctt gggcgtgctg ccgccgctct g
//
LOCUS       DASTZO010000185         3825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_194894,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000185 DASTZO010000000
VERSION     DASTZO010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_194894"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..670
                     /locus_tag="VFU20_05110"
     CDS             <1..670
                     /locus_tag="VFU20_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5285875.1"
                     /translation="FVNMLDAARLAGVRRFVYAASSSTYGDEPNLPKREERIGNPLSP
                     YAATKLADEIYASVYARSYDYPSIGLRYFNVFGPKQDPEGPYAAVIPKWIAAMLAGTA
                     IEIYGDGSTSRDFCYVANAVQANLRAALAPDEAQNQVYNVAVGERTSLNELFGLLRDG
                     LARHQVHYDQQPVHADFRRGDVRHSEADIAKARRLLGYAPTHRIADGIAEALPWYLRT
                     ARRA"
     gene            667..1008
                     /locus_tag="VFU20_05115"
     CDS             667..1008
                     /locus_tag="VFU20_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285876.1"
                     /translation="MNFLRGIDWYQVKLFAQHSTGISMDALHVIVGVVLQLLVAFLFR
                     SSVARPMPLLAVLAFALLNEANDLRIEIWPDPGMQFGEAVKDIVLTILLPTLVFLVAR
                     RRPRLLVQGSN"
     gene            complement(993..1760)
                     /gene="surE"
                     /locus_tag="VFU20_05120"
     CDS             complement(993..1760)
                     /gene="surE"
                     /locus_tag="VFU20_05120"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEU5285877.1"
                     /translation="MRILLTNDDGVNARGMALLEAVARKLADDIWIVAPSEEQSGAGH
                     SLTLTQPVRLRHHGEKRFSVTGTPTDAVMLALAHVMKDSPPDVILSGINRGANLGEDV
                     TYSGTVSAAMEGALAGVRSIALSQGYSREGMGDTVPFAAAEGWAERVLAPLLSFESAP
                     GTLINVNFPAVPAEEVKGIRVCRQGIRDYGRLRIVQRTDPRGYDYYWFGLGPMVETPG
                     HLTDLEAVADGFVSVTPLHLDLTHDESLLPLGRQFEP"
     gene            2155..2520
                     /gene="dksA"
                     /locus_tag="VFU20_05125"
     CDS             2155..2520
                     /gene="dksA"
                     /locus_tag="VFU20_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013834409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HEU5285878.1"
                     /translation="MCDKHRAYFLRKLKDWKDAIIEESRATMAQLQVDSLREPDLADR
                     ASSETDWAIELRTRDRQRKLIAKIDCAIRRLYEGEYGYCEVTGEPISLARLEARPVAT
                     MTLEAQERHERIEKVSRDD"
     gene            complement(2610..2765)
                     /locus_tag="VFU20_05130"
     CDS             complement(2610..2765)
                     /locus_tag="VFU20_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HEU5285879.1"
                     /translation="MDKAPVEDLASKHAALHALIDEEENRPHPDEDLLHRLKKEKLRL
                     KDAMLGH"
     gene            2931..3131
                     /locus_tag="VFU20_05135"
     CDS             2931..3131
                     /locus_tag="VFU20_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HEU5285880.1"
                     /translation="MNDDETATQQLAALKLEHRALDEQIAALVAEGATDQLELARLKK
                     RKLRLKDEIERMRDRSVPDIIA"
     gene            3227..3715
                     /locus_tag="VFU20_05140"
     CDS             3227..3715
                     /locus_tag="VFU20_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HEU5285881.1"
                     /translation="MDDSTDLFQPEARITPRLVDSFYTEAMLLADEARSYFDQAGREA
                     RLELDPFARVGFACESLKVTTRIMHIVAWLLTQRAIESGDIPARHGRRPERRLGHAQD
                     SDPAIVAQLPEAAQRLINASADLYSRVRRLDEGTMFEEPAQSPARALMGRLERGLGER
                     LH"
BASE COUNT          628 a         1298 c         1266 g          633 t
ORIGIN      
        1 ctttgtcaac atgctcgacg cggcgcggct cgccggagtc cggcgcttcg tctacgcggc
       61 ctcgagctcg acctacggcg acgagcccaa tttgcccaag cgcgaggagc ggatcggcaa
      121 cccgctgtcg ccctatgctg cgaccaagct cgccgacgag atctacgcgt cggtctatgc
      181 gcggagctac gattacccgt cgatcgggct gcgctacttc aacgtcttcg gccccaagca
      241 ggacccggaa ggcccctatg ccgcggtgat cccgaaatgg atcgcggcga tgctggccgg
      301 caccgcgatc gagatttacg gcgatggctc gaccagccgc gacttctgct acgtcgccaa
      361 tgcggtccag gccaatttgc gcgccgcgct cgcgcccgac gaggcgcaga accaggtcta
      421 caatgtcgcg gtcggcgagc ggacgtcgct gaacgagctg ttcggcctgc ttcgcgacgg
      481 gctggcgcgg caccaggtgc attacgacca gcagccggtc cacgccgact tccgccgcgg
      541 cgacgtgcgc cattcggaag ccgacatcgc caaggcgcgc cggttgctcg gctacgcgcc
      601 gacccaccgc atagccgatg gcatcgccga ggcgctcccc tggtacctgc ggacggcccg
      661 gcgcgcgtga acttcctgcg tggaatcgac tggtaccagg tcaagctgtt cgcccagcac
      721 tcgacgggca tcagcatgga cgctctgcac gtgatcgtcg gggtcgtgct gcaattgctg
      781 gtcgcgttcc tgttccggag ctcggtcgcg cggccgatgc cgctgctggc ggtgctggcc
      841 ttcgccctcc tcaacgaggc gaacgacctg cggatcgaga tctggcccga tccggggatg
      901 cagttcggcg aggcggtcaa ggacatcgtc ctgaccatac tgctcccgac cctggtcttc
      961 ctcgtcgccc ggcgccgccc gagactgctc gttcagggct cgaactgacg gccgagcggc
     1021 agcagcgatt cgtcgtgggt caggtcgagg tgcagcgggg tcaccgagac gaagccgtcg
     1081 gcgaccgcct cgaggtcggt cagatgcccg ggggtctcga ccatcgggcc cagaccgaac
     1141 cagtaataat cgtagccgcg cgggtcggtg cgctggacga tcctcaagcg gccgtaatcg
     1201 cggatcccct gccggcagac ccggatgccc ttgacctcct cggccgggac cgcggggaag
     1261 ttgacgttga tcagcgttcc gggcgccgac tcgaagctca gcagcggcgc cagaacgcgc
     1321 tccgcccagc cttccgcggc ggcgaacggg accgtgtcgc ccatgccctc gcgcgaatag
     1381 ccctggctca aggcgatcga gcggaccccg gcgagcgcgc cctccatcgc cgccgacacc
     1441 gtgcccgaat aggtcacgtc ctcgccgagg ttggcgccgc ggttgattcc cgacagaatc
     1501 acgtcgggcg gcgaatcctt catcacgtgc gcgagcgcca gcatcaccgc gtcggtcggg
     1561 gtgccggtga ccgaaaagcg cttctcgccg tggtggcgaa gccgcaccgg ctgggtcagg
     1621 gtcagcgaat ggccggcgcc cgactgctcc tcggacgggg cgacgatcca gatgtcgtcg
     1681 gcgagcttgc gcgcgaccgc ctcgagcagc gccatgccgc gcgcgttgac gccgtcgtcg
     1741 ttggtcagca ggatccgcat cgaaacggcg ctatgggcga gccggccgaa agccgcaacc
     1801 ggcgctcgca aagagcgaaa ttgcgccata ttggcaacat cttgcgcttt tcttgccgtg
     1861 ctcttcgtca gttcttgtta aggcgccggg gcttaactat aggcgcgttg gggatgaccg
     1921 gctttcccgg tcttaagtgg cgggaaagtc tagcgtaatc gcctccggct gcaccggtgg
     1981 ggcccggccg cagcggcggc ggcacgcgcg gccttcatcg aagcgtcttc gatgggccgc
     2041 gactggggag ggattatggc gaccgcactt gtcgacctgt atggcgaaca cccgctaccg
     2101 ttcgaccggc tgatcctggc cgagggctat tgcccgaccg agcgcgaaga attcatgtgc
     2161 gacaagcacc gcgcttattt ccttcgcaag ctgaaggact ggaaggacgc gatcatcgag
     2221 gaatcgcggg cgaccatggc ccagctccag gtcgattcgc tgcgcgagcc ggacctcgcc
     2281 gaccgggcgt cgagcgagac cgactgggcg atcgagctcc gcacccgcga ccgccagcgc
     2341 aagctgatcg ccaagatcga ttgcgccatc cgccgcctct acgagggcga atacggctat
     2401 tgcgaagtca ccggcgagcc gatctcgctg gcccggctcg aggcgcggcc ggtggcgacc
     2461 atgactctcg aggcgcagga gcgccacgag cggatcgaaa aggtgtcgcg cgacgactga
     2521 agcgcagccg aggcgggaca tcggcgcgct ggaagcagcc gccgcgcttt caggatcggt
     2581 cgtcttttaa gtcggacttc gggccggccc tagtggccga gcatcgcgtc cttcagcctc
     2641 agcttttcct tcttcagtcg gtggagcaaa tcctcgtcgg ggtgcgggcg attctcttcc
     2701 tcgtcgatga gcgcgtggag cgccgcatgc ttggatgcca gatcctcgac aggtgccttg
     2761 tccatcggcg ggtatccttt cctggttgaa agaccgccgg ccaataaatc acgattttgg
     2821 ccatttgtct cgggcgttca ttacggtttg gcgacaatcg ggcgcggact ggctcgcccg
     2881 gcggccgcga aattttccaa ttccacgcgc gaatcgatta ggctgccgga atgaacgacg
     2941 acgagactgc cacccagcag cttgccgcgt tgaagctcga gcaccgcgcc ctcgacgagc
     3001 agatcgcggc gctggtcgcg gaaggggcga ccgaccagct cgagcttgcc cggctgaaga
     3061 agcgcaagct tcgcctgaag gatgaaatcg agcgtatgcg cgaccgcagc gtgcccgaca
     3121 tcatcgcctg accgagtccc gcggcgggac gaattttccc cgaatcgcag ccgccgccat
     3181 tctggatcgt tttcagcggc ggtcatcggt ggcagttagg cgagcgatgg acgactcgac
     3241 cgaccttttt cagcccgagg cgcggatcac tccgcggctc gtggattcct tctacaccga
     3301 ggcgatgctg ctggccgacg aggcgcggtc ctatttcgac caagcggggc gcgaggcgcg
     3361 gctcgagctc gacccgttcg cgcgcgtcgg attcgcctgc gaatcgctca aggtcaccac
     3421 ccggatcatg cacatcgtcg cctggctgct gacccagcgg gcgatcgaga gcggcgacat
     3481 cccggcccgc cacggccgcc ggcccgagcg ccggctgggc cacgcgcagg acagcgaccc
     3541 ggcaatcgtc gcccagctgc ccgaagccgc gcagcggctg atcaatgcca gcgccgacct
     3601 ctattctcgt gtccgtcggc tcgacgaggg gacgatgttc gaggagccgg cgcaaagccc
     3661 ggcccgggcg ctgatggggc ggctcgagcg cggcctcggc gaacgattgc actaaggaca
     3721 gactagccgc agaccctggc cttcgcggcc gcatattctt ccgcaagctt cgcgaccagc
     3781 gccgcggccg gcatgatccg gtcgaccgcc ccgacgccct ggccc
//
LOCUS       DASTZO010000186         6358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_616359,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000186 DASTZO010000000
VERSION     DASTZO010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_616359"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..406)
                     /locus_tag="VFU20_05145"
                     /pseudo
     CDS             complement(53..406)
                     /locus_tag="VFU20_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009823317.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
     gene            701..1282
                     /locus_tag="VFU20_05150"
     CDS             701..1282
                     /locus_tag="VFU20_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010409041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEU5285882.1"
                     /translation="MPLVLASSSAIRRAMLEAAGVEHEAVPAGIDESAAKASNDDPEA
                     LALELAGAKAAAVSATRPADWVIGSDSLVSVDGRLFDKPADRAQAAEHLRLFSGRAMR
                     LTSAVALARGGAVEWSQCDGATLHVRELSDAFIAGYLTSEWPAVSHCVGVFRIEGPGV
                     QLFERIEGDHFTIQGMPLLPLLGALRERGVIPS"
     gene            1296..1889
                     /gene="coaE"
                     /locus_tag="VFU20_05155"
     CDS             1296..1889
                     /gene="coaE"
                     /locus_tag="VFU20_05155"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967536.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU5285883.1"
                     /translation="MIKIALTGSIGMGKSTVARMFEQRGVPLFDADAEVRRLQGPGGA
                     LVEPIGRRFPGSVQGGAVDREALSALAIGIPDELAALEAIVHPAVQQARSQFIADHAG
                     APALLFDIPLLFETGGEAAFDTVVVVSAPPEVQRQRVLSRPGMTSAKLDTILVRQMPD
                     AEKRRRADFVVDTGGPIEQTERQVEHILACLGLAAGR"
     gene            1897..2586
                     /gene="dnaQ"
                     /locus_tag="VFU20_05160"
     CDS             1897..2586
                     /gene="dnaQ"
                     /locus_tag="VFU20_05160"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HEU5285884.1"
                     /translation="MREIVFDTETTGLNPAGGDRMVEIGCIEIFNRVETGRHFHAYFN
                     PERPMPSEAEAVHGLTTVFLSDKPRFGERAGELLDFLEDSPLVAHNASFDFGFLNHEL
                     ERCGRPSVCTSRMVDTLVLARSRHPGAKHSLDALCARFGVDRSQRVRHGALLDAQLLA
                     QVYVELTGGRQIGLGLVADTDGADVAVGGAAVTLREPRAARPHGAAAEELERHRAFIA
                     QLVNPLWARFA"
     gene            2668..3228
                     /gene="raiA"
                     /locus_tag="VFU20_05165"
     CDS             2668..3228
                     /gene="raiA"
                     /locus_tag="VFU20_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEU5285885.1"
                     /translation="MDVRVAGHQVDTGESLREHATRRIEEITEKYFARAIAANVTFGR
                     GPNNDFTCDIVAPVAQGVVLKASHRAQEAQLAFNGAADRIEKQLRRYVKRLREHKADG
                     EPYIENAGYTVFSAPQESDEAGAHPAIIAETRVDIPEASVSDAVMLMDLRNTNALLFK
                     NSASGALNMIYRREDGNIGWVEPQGS"
     gene            3295..3759
                     /locus_tag="VFU20_05170"
     CDS             3295..3759
                     /locus_tag="VFU20_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5285886.1"
                     /translation="MQLAEFLDFDAIKTSLSGGSKRSLLQQLATLAGQRLGIDSAAIL
                     ASLSERERLGSTGFGQGVAIPHGKIEGLTRIYGLFVRLAEPVDYKALDKAPVDLVFLL
                     LSPPEAGAEHLKALAAISRVTRHAATLEKMRGARSRDALAAVLMGADERDAA"
     gene            3799..4128
                     /locus_tag="VFU20_05175"
     CDS             3799..4128
                     /locus_tag="VFU20_05175"
                     /inference="COORDINATES: protein motif:HMM:NF019019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1491 family protein"
                     /protein_id="HEU5285887.1"
                     /translation="MPASVEAAGLVRRAQGSGDFATVIKKGDPDRGAILLVVSSRGRH
                     VACLERMLTLDGGYRWHAVGPSESAGSDEIRDFLAKRTRFDADLWAIELDIAEPERFI
                     AETSLTG"
     gene            4307..4966
                     /locus_tag="VFU20_05180"
     CDS             4307..4966
                     /locus_tag="VFU20_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEU5285888.1"
                     /translation="MLPSLRSALLVFAALSASSVAPAQVGPAAEPLIPIPTVAIPDTV
                     VQSAPAPQPKEVLAPSEAVEAPEADELAPLPATPAKPSGDLASLVAQLRGSDPGSREL
                     ECLAVGIYFESKSEPLAGQLAVGEVIANRANSKGRFPSSYCGVLFQRSQFSFIRGRAL
                     PSVPRSSRQWQTAVAIAKIVDQDLKDSAANNALFFHARRVSPRWKLKRVAAIGNHIFY
                     R"
     gene            5092..5586
                     /locus_tag="VFU20_05185"
     CDS             5092..5586
                     /locus_tag="VFU20_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HEU5285889.1"
                     /translation="MSTAPDSLFQPCFADAPPIAGEVARGVTRLFCRRDLFAICEVPL
                     PNGRRADMMAIDPKGALTIVEIKVARADLVNDCKWTDYLDYCDHFYWAVPPFLAAILD
                     HERYLPGAAGLIVADRYDAAIMRDAVHRPLAPARRKAELLRFARRAARRLSAQIDPTL
                     GEGN"
     gene            complement(5583..6191)
                     /locus_tag="VFU20_05190"
     CDS             complement(5583..6191)
                     /locus_tag="VFU20_05190"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU5285890.1"
                     /translation="MNRARMAAAALAMVLIAQPAGAQGGGFDGQKFVQAVRDRDGNEA
                     MELLRARGTTVLNARDERGDTGLIVAISRRDENWAMFLLAEGADPNFPAKSGDTPLIA
                     AARVGYTGAAEQLLELDVKVDAANRMGETALIVAVQQRHAPIVRLLLAAGADPDRTDS
                     AAGLSARDYARRDTRARDILKLIESAKSKQKADPGPKASSIR"
BASE COUNT          985 a         2235 c         2136 g         1002 t
ORIGIN      
        1 tggccaccgt ctcgtagctc ggctcgagcc gtgtcgggta cgggctcagc tagtcgagct
       61 cggccccagc ccgcggaggc gtcagccgcg gcccatcgcc ggcgacgaac gacagattct
      121 ggccgagcgc gaatgggacg atgagaatgt ccgagaacag gatcgccgcg tcgagcggga
      181 agcgcttgag gggctggaga gtcacctccg ccgcggcgtc gctgtcgtag accaggtcga
      241 ggaacccgcc cttggactcg cgcagctcgc ggtactcggg cagatagcgc cctgcctggc
      301 gcatcatcca gatcggcgga ggatcgcgcc gctcaccctt caggacggta agcaagggct
      361 tggccgccga ggggtcgtcc ggagcttcac tcgggctcga ggccaccccc ttattctccc
      421 ttattatata tatagaagaa agattgttga tgatgatggc ggccgggacg cggggtttag
      481 gcgcagcttc cggaattgcc aacaagcggg gttccggggc cgtctcggga atccgccgag
      541 tcgcccgcgg ccgtgcccgc gattcacagg ctggggagca aatatcgcgc ccgtggatga
      601 atcgtcggac aaggcgcgac ggcctgggca caagcgcagc gttgagcccg cccccatcgc
      661 cgcgctagga cacaggccca tctgtccaca aggttatgcc gtgccgctcg tgctcgcctc
      721 atcaagcgcc atccgccggg cgatgctcga ggcggccggg gtcgagcatg aggcggtccc
      781 cgccggaatc gacgaatccg ccgcgaaggc aagcaacgac gacccggaag ctctggcgct
      841 cgagctcgcc ggcgccaagg cggcggcggt cagcgccacg cggcccgcgg attgggtgat
      901 cggcagcgat tcgctggtca gcgtcgatgg ccggctgttc gacaagcccg cggaccgcgc
      961 ccaggcggcc gagcatctgc gcctgttctc gggccgggcg atgcggctga ccagcgcggt
     1021 cgccctggcg cgcggcgggg cggtcgagtg gagccagtgc gacggcgcga ccctccacgt
     1081 ccgcgagctc tccgacgcgt tcatcgccgg ctacctgacg agcgaatggc cggcggtgtc
     1141 gcactgcgtc ggagtgttcc ggatcgaggg tcccggcgtg caattgttcg agcgcatcga
     1201 gggcgaccat ttcaccatcc agggcatgcc gctgctgccg ctgctcggcg cgctgcgcga
     1261 gcgcggggtg atcccttcat gactggaatc ggcgcatgat caagatcgcg ctcaccgggt
     1321 cgatcggcat gggcaagtcg accgtcgccc ggatgttcga gcagcgcgga gtgccgctgt
     1381 tcgacgccga tgccgaggtg cgccggctgc aggggcccgg cggcgccctg gtcgagccga
     1441 tcgggcggcg cttcccgggc agcgtccagg gcggggccgt cgaccgcgag gcgctgtcgg
     1501 cgctggccat cgggatcccg gacgagctcg cggcgctgga ggcgatcgtc cacccggcgg
     1561 tgcagcaggc gcgctcgcaa ttcatcgccg accacgccgg cgcgcccgcc ttgctgttcg
     1621 acattccctt gctgttcgaa accggcggcg aggccgcgtt cgacacggtg gtcgtcgtct
     1681 ccgcgccgcc cgaggtgcag cgccagcgcg tgctgtcgcg gccggggatg acgagcgcca
     1741 aactggacac gatcctcgtc cgccagatgc ccgacgccga aaagcgccgg cgcgccgatt
     1801 tcgtcgtcga caccggcggt ccgatcgagc agaccgagcg ccaggtggag catattctcg
     1861 cttgtctcgg actcgccgcg ggtcgataac ggggccgtgc gggaaatcgt attcgacacc
     1921 gagacgaccg ggctcaaccc cgccggcggc gaccggatgg tcgagatcgg ctgcatcgag
     1981 atcttcaacc gggtcgagac cggccgccat ttccacgctt atttcaaccc cgagcggccg
     2041 atgccgagcg aggccgaggc ggtccacggg ctgaccaccg tcttcctgtc ggacaagccg
     2101 cggttcggcg agcgcgccgg cgaactgctc gacttcctcg aggattcgcc gctggtcgcg
     2161 cacaatgcga gcttcgattt cggcttcctg aaccatgagc tcgagcgctg cggccggcca
     2221 agcgtctgca ccagccggat ggtcgacaca ttggtgctcg cgcgcagccg ccacccgggc
     2281 gccaagcaca gcctcgacgc cttgtgcgcg cgcttcggcg tcgaccgcag ccagcgcgtc
     2341 cggcacggcg ccctgctcga cgcgcaattg ctcgcccagg tctatgtcga gctgaccggc
     2401 ggccgccaga tcggcctcgg cctggtcgcc gacaccgacg gcgccgatgt cgccgtcggc
     2461 ggcgcggcgg tcacccttcg cgagccccgc gcggctcgtc cccatggcgc cgccgccgag
     2521 gaactggagc gccaccgcgc gtttatcgcc cagctggtca acccgctgtg ggcccgcttc
     2581 gcctaggaac aggcgggccg agttgacggg gggttccggc cagccctaag cggctgcgcc
     2641 gccaacatgg aagaggagcc tggccaagtg gacgttcggg tcgcgggtca tcaggtcgac
     2701 accggggaat cgctgcgcga gcatgcgacc cggcggatcg aggagatcac cgagaaatat
     2761 ttcgcccggg cgatcgccgc caacgtcacc ttcggccgcg gccccaacaa cgacttcacc
     2821 tgcgacatcg tcgctccggt cgcgcagggc gtggtcctga aggcgtcgca ccgggcgcag
     2881 gaggcgcagc tggccttcaa cggcgccgcc gaccgcatcg aaaagcagct gcgccgctac
     2941 gtgaagcggc ttcgcgagca caaggccgac ggcgagccct atatcgagaa tgccggctac
     3001 acggtgtttt ctgcgcccca ggaaagcgac gaggcgggcg cgcaccccgc gatcattgcc
     3061 gaaacccggg tcgacattcc cgaggccagc gtatccgacg cggtgatgct gatggacctt
     3121 cgcaacacca atgcgctgtt gttcaagaat tcggcgtcgg gcgccctcaa catgatttac
     3181 cgacgagagg acggaaacat cggctgggtc gagccgcagg gctcataaag ccccatttcc
     3241 aatcgcggcc ggcgtgcgat gcaccggtcc gcccgttcct gaaagtgcgt accgatgcaa
     3301 cttgccgagt tcctcgattt cgacgcgatc aagaccagcc tgtcgggcgg gagcaagcga
     3361 tcgctgctgc agcagctcgc gaccctcgcc gggcagcggc tcggaatcga ttccgccgcg
     3421 atcctcgcca gcctgtccga gcgcgagcgc ctgggctcga ccggcttcgg ccagggagtc
     3481 gccatcccgc acgggaagat cgagggtttg acgcgcattt acggcctgtt cgtgcggctc
     3541 gccgagccgg tcgactacaa ggcgctcgac aaggcgcccg tcgacctcgt cttcctgctc
     3601 ctgtcgccgc ccgaggccgg ggccgagcat ttgaaggcgc tcgccgcgat cagccgggtg
     3661 acccgccacg cggcgaccct cgagaaaatg cgcggggcgc gcagccgcga cgctctggcg
     3721 gcggtgctga tgggggccga tgagcgcgac gccgcctgaa ccttcggcgc cgcccgaatc
     3781 cgcatgaccg ccgatcgcat gccggcgtcg gtcgaggccg caggactggt ccggcgcgcc
     3841 cagggcagcg gcgatttcgc gaccgtcatc aagaaaggcg accccgaccg cggcgcgatc
     3901 ctgctggtcg tcagcagccg cggccgccac gtcgcctgcc tcgagcgcat gctgaccctc
     3961 gatggcggct atcgttggca cgccgtcggt ccatccgaat cggcgggttc cgatgaaatc
     4021 cgcgatttcc tcgcgaagcg cacccggttc gacgcggatt tgtgggcgat cgaactggat
     4081 atcgcggagc cggaacgatt catcgctgaa acatccttaa cgggttgacc acctcgttcc
     4141 ggacccggac aagcgagccc aacaccaaga gcggggggca tttcccgacc gaaaattcgg
     4201 cggaaaatgc cacgcgagac ggggaacaac ccaaggggcc aaagcgggat tttccgccgg
     4261 tacggcccgc gagttcgggc ggttcaccgc tgatttcacg gtatccatgt tgccttcgct
     4321 tcgttcagca ctgctggtct tcgccgcgct cagcgcgagc tcggtcgccc ccgcccaggt
     4381 cgggccggcc gccgaaccgc tgatcccgat tcccaccgtc gcgatccccg acacggtggt
     4441 ccagtcggcg ccggcgccgc aacccaagga agtgctcgct cccagcgaag ccgtcgaagc
     4501 gcccgaagcc gacgaattgg cgccgcttcc cgcgaccccc gccaagccga gcggcgacct
     4561 cgcctcgctg gtcgctcagc tgcgcggctc cgacccgggc agccgcgagc tcgagtgcct
     4621 cgcggtcggc atctatttcg aatcgaagag cgagccgctg gccggccagc tcgcggtcgg
     4681 cgaagtcatc gccaaccgcg ccaattccaa ggggcgcttc ccgtcgtcct attgcggggt
     4741 gctgttccag cgcagccaat tctcgttcat ccgcggccgc gctctaccga gcgtgccgcg
     4801 ctcgagccgc cagtggcaga ccgccgtcgc gatcgccaag atcgtcgacc aggacctgaa
     4861 ggattcggcc gccaacaacg ctctgttctt ccacgccagg cgggtgtcgc cgcgctggaa
     4921 gctcaagcgc gtcgccgcga tcggcaatca catcttctac cgctgacgcg gccatccccg
     4981 tcatcccagc ccaggctggg atcccagcga gttctcgccc tgcggccccc ggcgttcgcc
     5041 ggggcgacgg cggcggtttc cctaattctt gttttgttct aatcgtcgct cgtgagcacc
     5101 gcgcccgatt ctcttttcca gccctgcttc gccgacgctc cgccgatcgc cggggaggtc
     5161 gcgcgcggag tcacccgatt gttctgccgt cgcgacctgt tcgcgatctg cgaggtgccg
     5221 ctgcccaacg gccgccgcgc cgacatgatg gcgatcgacc ccaagggcgc cctgacgatc
     5281 gtcgagatca aggtcgcccg ggccgacctg gtcaacgact gcaagtggac cgactatctc
     5341 gactattgcg accatttcta ctgggcggtg ccgcccttcc tcgccgcgat cctcgaccat
     5401 gagcgctacc tgccgggcgc cgccgggctg atcgtcgccg accgctacga cgccgcgatc
     5461 atgcgcgacg ccgtgcaccg gccgctggcg ccggcgcgcc gcaaggccga gttgctgcgc
     5521 tttgcccggc gcgccgcccg gcgcctgtcg gcgcagatcg acccgacgct cggcgaaggc
     5581 aattaacgga tgctgctggc cttggggccg ggatcggcct tctgcttcga tttcgccgat
     5641 tcgatcagct tcaggatgtc gcgcgctcgg gtgtcgcgtc tggcataatc gcgcgccgac
     5701 agcccagccg cggaatcggt ccggtcgggg tcggccccgg cggcgagcag gagccggacg
     5761 atcggcgcat ggcgctgctg gacggcgacg atcagcgccg tctcgcccat ccggttggcg
     5821 gcgtcgacct tgacgtcgag ctccagcagt tgctcggcgg cgccggtata gccgacccga
     5881 gcggcggcga tcagcggggt gtcgccgctt ttcgccggga agttggggtc ggcgccctcg
     5941 gcgagcagga acatcgccca attctcgtcg cgcctcgaaa tggcgacgat caggccggtg
     6001 tcgccgcgct cgtcgcgggc gttgagaacc gtggtgccgc gtgcgcgcaa cagctccatc
     6061 gcttcgttgc cgtcgcggtc gcggaccgcc tggacgaact tctgcccgtc gaagccgccg
     6121 ccctgcgctc ccgccggctg ggcaatcagc accatcgcca gcgccgccgc ggccattctc
     6181 gcccggttca taagcctttg tttcccaatc aaccccgctt gatcggcggc tgatagcaga
     6241 ccatggataa gctggccatg aacaagctcc cgtcatccaa cgagaaaata ccgttctggg
     6301 agcggcctct ggcgagcctc gaccgcggcc agtgggaagc gctgtgcgac ggctgcgg
//
LOCUS       DASTZO010000187         5177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_747023,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000187 DASTZO010000000
VERSION     DASTZO010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5177
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747023"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFU20_05195"
     CDS             <1..335
                     /locus_tag="VFU20_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU5285891.1"
                     /translation="MYNFTLPSQLKAWIDRILIAGKTFRYTADGPEGLAGGKRVIVAV
                     SRGGFYDDSSALEHVETYLKGVFGFIGITPEFVHADGVNFGPEQREAGIANGLAEVER
                     LEFERLAA"
     gene            complement(337..1659)
                     /locus_tag="VFU20_05200"
     CDS             complement(337..1659)
                     /locus_tag="VFU20_05200"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HEU5285892.1"
                     /translation="MARYEFKLPDIGEGIAEAEIVAWHVKVGDRVEEDQQLADMMTDK
                     ATVEMETPVAGKVVELAGEVGDQIPIGSVLAVIETDGAAAAEESQKEVPIADGAERPT
                     AAQAEAMPSVDVEVAPRPPAGGEKPRGSEGAGLSKKPAARSEASPTPAPPAKERGGHV
                     LASPAVRARARDLGIDLAQVKTAGDRVRHSDLDAFLLYNGGSVSGRGAATRGDETIKV
                     VGLRRKIAENMAEAKRRIPHFTLVEEFDVTALEETRAMMNRDRGDHPKLTLLPFLITA
                     MGKAVADWPMLNATFDDEAMALTRHGAMHLGMATQTEGGLMVPVIRNAQAMSVWQLAA
                     EILRLAEAARSGKASRDELTGSTITLSSLGPMGGITSTPVINRPEVAIIAVNKVEEKV
                     VVIDGEIEVAKRMNLSLSCDHRVVDGWDAASFLQALKALIENPLRLLS"
     gene            complement(1659..2663)
                     /locus_tag="VFU20_05205"
     CDS             complement(1659..2663)
                     /locus_tag="VFU20_05205"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU5285893.1"
                     /translation="MNMIQAINSAHKVMMARDPDVVVFGEDIGYFGGVFRATEGLQRE
                     FGSTRVFDAPISEGGIVAMAVGMAAYGLRPVVEIQFADYIYPGYDQIVSEVAKIRYRT
                     AGEWAMPMVIRSPYGGGIFGGQTHSQSPESLFTHIAGLKTVIPSTPHDAKGLLIAAIE
                     DPDPVIFFEPKRIYNGPFDGHHDRPVKPWAKHPASDVPDGHYSVPLGKAAVVRKGEAL
                     TLLAYGTMVHVALAAVEEQGVDAEVIDLRTLVPLDIATIEASVEKTGRCLVVQEAPRT
                     SGFGAELAALVQERCFYHLESPVERVTGWDTPYPHSYEWAYFPGPKRMKRALEKVMAD
                     "
     gene            complement(2678..3952)
                     /locus_tag="VFU20_05210"
     CDS             complement(2678..3952)
                     /locus_tag="VFU20_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase
                     (2-methylpropanoyl-transferring) subunit alpha"
                     /protein_id="HEU5285894.1"
                     /translation="MSDRDPSKLKPNRPSLALHVPEPPFRPGDKPDFSSLRIPAAGEA
                     PRPDVAAPPADTHPLTTHLVRVLDDQNRAVGPWDPKLDADTMRKMLRDMVTVRIFDDR
                     MYRAQRQGKTSFYMKCTGEEAIAVAAAAALDRDDMHFPTYRQQGLLVARGYPLTEMMC
                     QIYSNKGDKLRGRQLPIMYSDKAHGFFSISGNLGTQFPQAVGWAMASAIKGDSRIAMG
                     WIGDGATAEGDFHAGMTFAAVYNAPVVLAVVNNQWAISSFSGIAGAERATFAQRAVGY
                     GIAGMRVDGNDALAVYAAVRWAADRARSNLGPSLIEFFTYRAEGHSTSDDPSGYRPVN
                     EAKAWPLGDPVERLKAHLVGLGEWDEDRHSAMAAECDAMVRAAQKEAETLGILPQQGK
                     DDIGSMFEDVYADMPWHIAEQRDAALAESKSE"
     gene            4062..4349
                     /locus_tag="VFU20_05215"
     CDS             4062..4349
                     /locus_tag="VFU20_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3297 family protein"
                     /protein_id="HEU5285895.1"
                     /translation="MADQPKDLPDRLSLDPRSPFFDEDVLSRGVGIRFNGQEKTNVEE
                     YCISEGWIRVAAGRSRDRYGNPMTIKLNGKVEPYVQDPAQPEGGEGVASGG"
     gene            complement(4310..4540)
                     /locus_tag="VFU20_05220"
     CDS             complement(4310..4540)
                     /locus_tag="VFU20_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285896.1"
                     /translation="MLGKIAGALIGRRLAGRHSGAKGAILGYGAAALARRGLGPLAAV
                     AALGWGAKKLYERRRGRSPAYPPDATPSPPSG"
     gene            4787..4924
                     /locus_tag="VFU20_05225"
     CDS             4787..4924
                     /locus_tag="VFU20_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285897.1"
                     /translation="MSGQSKISRTLVAAIGALLMSSVTVGAAVGPAQTVANPVGVTLH
                     A"
     gene            4917..>5177
                     /locus_tag="VFU20_05230"
     CDS             4917..>5177
                     /locus_tag="VFU20_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEU5285898.1"
                     /translation="MLELGVIEARVPNRTPRMPERQPERPTARFAPLAALFRGPIFSR
                     TRDIFAANMTELLDRSEDPARMIRMIILEMEETLVEVRASAAR"
BASE COUNT          754 a         1796 c         1739 g          888 t
ORIGIN      
        1 cgatgtacaa tttcaccttg ccgagccagc tcaaggcgtg gatcgatcgc atcctgatcg
       61 ccggcaagac cttccgctac accgccgacg gccccgaagg cttggccggc ggcaagcgcg
      121 tcatcgtcgc ggtgtcgcgc ggcggttttt acgacgactc gagcgcgctc gagcatgtcg
      181 agacctattt gaagggcgtg ttcggcttta tcggcatcac gcccgagttc gtccatgctg
      241 acggagtcaa ttttggcccc gaacagcgcg aagcgggaat cgccaacggg ctggcggaag
      301 tcgagcggct ggaattcgag cggctggcgg cctgagctac gacagcaggc gcagcgggtt
      361 ttcgatcagc gccttcagcg cctgaagaaa gctcgccgcg tcccagccgt cgaccacgcg
      421 gtgatcgcag gacaaagaca ggttcatccg cttggcgact tcgatctcgc cgtcgatcac
      481 gacgaccttc tcctcgacct tgttgacggc gatgatcgcg acctcgggcc ggttgatcac
      541 cggggtcgag gtgatcccgc ccatcgggcc gaggctcgac aaggtgatgg tcgatcccgt
      601 cagctcgtcg cggctcgcct tgccggagcg cgcggcctcg gcgaggcgca ggatctccgc
      661 ggccagctgc cagacgctca tcgcctgcgc gttgcggatc accggcacca tcagcccacc
      721 ctcggtctgg gtcgccatgc ccagatgcat cgcgccgtga cgggtgagcg ccatcgcctc
      781 atcgtcgaag gtcgcgttga gcatcggcca gtcggcgacc gccttgccca tcgcggtgat
      841 caggaacggc agcagggtca gcttgggatg gtcgccgcgg tcgcggttca tcatcgcccg
      901 ggtctcctcg agcgcggtga cgtcgaactc ctcgaccagg gtgaaatgcg ggatccggcg
      961 cttcgcctcg gccatgttct cggcgatctt gcggcggagc ccgacgacct tgatcgtctc
     1021 gtcgccccgg gtcgccgcgc cgcggcccga caccgagccg ccgttgtaga gcaggaacgc
     1081 gtcgaggtcg gagtgacgca cccggtcgcc ggcggtcttc acctgcgcaa ggtcgatgcc
     1141 gaggtcgcgc gcccgcgcgc gcacggcggg tgaagcgagg acgtgtcctc ccctctcttt
     1201 agcgggaggg gccggggttg ggcttgcctc gctcctggcc gcaggctttt tcgacaggcc
     1261 cgccccttcg ctgccgcgcg gcttttcccc tcccgcgggc gggcggggag caacttccac
     1321 atcgacgctc ggcatcgctt cggcctgggc cgcggtcggc cgttccgcgc cgtcggcgat
     1381 gggcacttcc ttttgcgatt cctcggcggc cgccgcgcca tcggtctcga tcaccgcgag
     1441 caccgagccg atcgggatct ggtcgccgac ctccccggcg agctcgacca ccttgcccgc
     1501 gaccggggtc tccatctcga ccgtcgcctt gtcggtcatc atgtcggcga gctgctggtc
     1561 ttcctcgacc cggtcgccga ccttgacgtg ccaggcgacg atctcggcct cggcgatgcc
     1621 ctcgccgatg tcgggcagct tgaactcgta tcgcgccatc agtccgccat cactttctca
     1681 agcgctcgct tcatccgctt cggccccggg aaataggccc actcatagga gtgcggatag
     1741 ggggtgtccc agccggtgac ccgctcgacc gggctttcga gatgatagaa gcagcgctcc
     1801 tggaccaggg ctgcgagctc ggcgccgaag ccgctggtgc gcggcgcttc ctggacgacc
     1861 aggcagcggc cggtcttctc gaccgaagcc tcgatcgtcg cgatgtccag cgggaccagc
     1921 gtcctcaaat cgatcacctc ggcgtcgacg ccctgctcct cgaccgcggc gagggcgaca
     1981 tggaccatcg tcccgtaggc gagcagggtc agcgcctcgc ccttgcggac caccgccgcc
     2041 ttgccgagcg gcacgctata gtggccgtcg gggacgtcgc tcgccgggtg cttggcccac
     2101 ggcttgaccg gtcggtcgtg gtggccgtcg aacgggccgt tgtagatgcg cttgggctcg
     2161 aagaagatca ccgggtcggg atcctcgatc gccgcgatca gcaggccctt ggcgtcgtgg
     2221 ggcgtcgacg ggatcaccgt cttcaggccg gcgatgtggg tgaacaggct ttcggggctc
     2281 tggctgtggg tctggccgcc gaagattcct ccgccgtagg gcgagcggat gaccatcggc
     2341 atcgcccatt cgcccgcggt ccggtagcgg atcttggcga cctcgctgac gatctggtcg
     2401 tagccgggat agatatagtc ggcgaactgg atctcgacga cgggcctcaa gccataagcg
     2461 gccatcccga ccgccatcgc gacgatcccg ccttcggaaa tcggagcgtc gaacacgcgg
     2521 gtcgagccga actccctctg cagcccctcg gtggcccgga acacgccgcc gaaatagccg
     2581 atgtcctcac cgaacacgac gacgtcggga tcgcgcgcca tcatcacctt gtgggcgctg
     2641 ttgatcgcct ggatcatgtt catctgcggc actcggctta ctcgcttttg ctctcggcga
     2701 gcgccgcgtc gcgctgctcg gcgatatgcc acggcatgtc ggcatagacg tcctcgaaca
     2761 tgctgccgat gtcgtccttg ccctgctgcg gcaggatgcc gagcgtctcg gcctccttct
     2821 gcgcggcgcg gaccatcgcg tcgcactcgg ccgccatcgc cgaatggcgg tcctcgtccc
     2881 attcgcccag gccgaccaga tgcgccttca ggcgctcgac cgggtcgccg agcggccacg
     2941 ccttggcctc gttgaccggc cggtagccgg acgggtcgtc ggaggtcgaa tggccctcgg
     3001 cgcgataggt gaagaactcg atcaggctcg gcccgaggtt ggagcgggcg cggtcggccg
     3061 cccagcgcac cgcggcataa acggcgagcg cgtcgttgcc gtcgacgcgc atcccggcga
     3121 tcccgtagcc gaccgcgcgc tgggcaaagg tcgcgcgctc ggcgccggcg atcccggaaa
     3181 agctcgagat cgcccactgg ttgttcacca ccgcgagcac gacgggcgcg ttgtagaccg
     3241 cggcgaaggt catcccggcg tggaagtcgc cctcggccgt cgccccgtcg ccgatccagc
     3301 ccatggcgat gcggctgtcg cccttgatcg ccgaggccat cgcccagccg accgcctgcg
     3361 ggaattgcgt gccgagattg cctgagatcg agaagaagcc gtgcgccttg tccgaataca
     3421 tgatcggcag ctggcggccc cgcagcttgt ctcccttgtt cgagtagatc tggcacatca
     3481 tttcggtcag cggatagccg cgggcgacca gcagcccctg ctggcggtag gtcgggaagt
     3541 gcatgtcgtc gcggtcgagc gccgccgcgg cggcgaccgc gatcgcctcc tcgccggtgc
     3601 acttcatgta gaagctggtc ttgccctgcc gctgggcgcg gtacatgcgg tcgtcgaaga
     3661 tgcgcacggt gaccatgtcg cgcagcatct tgcgcatcgt gtcggcgtcg agcttcgggt
     3721 cccacgggcc gaccgcccgg ttctgatcgt cgagcacgcg caccaggtgc gtggtcagcg
     3781 gatgcgtgtc ggccggcggc gccgcgacat cgggccgcgg ggcttcgccc gcggcgggga
     3841 tccggagcga cgagaagtcc ggcttgtcgc ccggccggaa cggcggctcg ggcacgtgca
     3901 gcgcaaggct cggccggttc ggcttcagct tcgaggggtc cctgtcgctc atcggatttc
     3961 agcggcgctc atctgcggca agcgcttaat cgccccgcgc ggggtcggca actgcgcgcc
     4021 cgcgttgacg tttctcggcc cgcgcggcta ggggcgcggc gatggccgac caacccaaag
     4081 atctgcccga ccggctgtcg ctcgacccgc gcagcccgtt cttcgacgag gacgtgctga
     4141 gccgcggcgt cggcatccgc ttcaacggcc aggaaaagac caacgtcgag gaatattgca
     4201 tttccgaagg ctggatccgc gtcgccgcgg gccgcagccg cgaccgctac ggcaacccga
     4261 tgaccatcaa gctcaacggc aaggtcgagc cctatgtcca ggacccggct cagccggagg
     4321 gcggcgaggg agtcgcgtcc ggaggatagg ccggcgaccg gccgcggcgc cgctcgtaca
     4381 gcttcttcgc tccccagccg agcgcggcca ccgcggccag cggcccgagc ccgcgccgag
     4441 cgagcgccgc ggctccgtac cccaggatcg cgcccttggc gccgctgtgg cggccggcga
     4501 gccgcctgcc gatgagtgcc ccggcgatct tgcccagcat gccgatgtcc tttcgaaaaa
     4561 ctgctcgggc gttcaagcgc cggcgggccg agtgggttcc cgccatccag cgggcccggg
     4621 ggttggtgcg tttcgccaag tcttggttgg tgaaacctcc caacccgccc tgaaggcgcc
     4681 gctttccaag aatttttcgc agttttgcgc gattggcacg cgccttgaat tgtaccgggc
     4741 atcgaccccg agatgatcag cggtcgaaaa aaagggagtt tgaaaaatgt ccggtcagtc
     4801 gaagatttcg cggaccctgg ttgcagccat cggcgccttg ctcatgagct cggtcaccgt
     4861 cggcgccgcc gtcggccccg cgcagaccgt tgccaacccg gtcggcgtca ccctccatgc
     4921 ttgagcttgg agtgatcgag gcccgggtgc cgaaccgcac cccgcgcatg cccgagcgcc
     4981 agcccgagcg gccgaccgcc cgcttcgcgc cgctcgcagc gctgttcagg ggcccgatct
     5041 tctcccgcac gcgggacatt ttcgcggcga acatgaccga gcttctcgac cgttcggagg
     5101 atccggcgcg gatgatccgg atgatcatcc tcgagatgga ggagactctg gtcgaggtgc
     5161 gcgcctcggc ggcgcgc
//
LOCUS       DASTZO010000188         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1112749,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000188 DASTZO010000000
VERSION     DASTZO010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112749"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VFU20_05235"
     CDS             <1..251
                     /locus_tag="VFU20_05235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285899.1"
                     /translation="LSVTMTQLLTIYADFQNADREGYVRLNTEGTQADLCRVGLALSE
                     GLRLCLSDGELRTTAVVVQPGTEGVWRAKIDWSGLSQE"
     gene            complement(314..1423)
                     /locus_tag="VFU20_05240"
     CDS             complement(314..1423)
                     /locus_tag="VFU20_05240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5285900.1"
                     /translation="MPDNVCDILIVGGGIAGASLGARLAGRARIMLIESEDLCGRHST
                     GRSAAFWVASLGLSPARRLSIASKPMFDERWPGTPTPLLRRRGTLHLTGAHGDTVEGA
                     EDLVGEHALVWLDRAELDAMVPGLREQWTGAWYEPSCADIEVAAFHSACLAAVRRQGG
                     EVRTDAALLSAKRSGDGWQVETAAGPVRAGLLVNAAGAWADDVAARCGVAPIGLQPMR
                     RTVVQLRIGRCGLRDMPLVTDLHASFYFKGEGERSVWVSPLDETPVDPCDAAPEEIDV
                     ATAIDRFERAVDWPVEAVERKWAGLRTFAPDRLLKLGFDPQEPGFFWCAGQGGMGIQT
                     APAASLLCAALIRGEELPKELAGIDPADFAPRGQP"
     gene            1494..1967
                     /locus_tag="VFU20_05245"
     CDS             1494..1967
                     /locus_tag="VFU20_05245"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU5285901.1"
                     /translation="MINAGFTELLLGVLAILLLVAAVVDVRTFTISNSLNLAIALLAP
                     LFWLSIALPLWPNVAIQLAVGAGVFALLAGAFYAGMMGGGDVKLAAALALWFSPASTV
                     KFLVLMSLAGGVLTLAMVAHHRMRGKAGRPEIPYGVAIAFGGLAILTQRFLNQFA"
BASE COUNT          325 a          732 c          710 g          348 t
ORIGIN      
        1 tattgtccgt gacgatgact cagctgctga cgatctacgc ggacttccag aatgccgata
       61 gagagggcta tgtccggctg aataccgaag gcacacaggc agacctctgc cgtgtgggtc
      121 tagcgctgtc ggaaggacta cgactttgcc tctcagatgg agagttgcgg accaccgcag
      181 ttgttgtgca acccggcaca gagggcgtct ggcgcgctaa gatcgactgg agcggcctct
      241 cccaggaata acctgatgtc cgctttgcac ccaaaggcgg acattgcaca ttgctggaga
      301 gccggcgcga atcctagggt tgcccgcgcg gcgcgaagtc ggcggggtcg atccccgcca
      361 gctctttcgg cagctcctct cccctgatca gcgccgcgca cagcaatgag gccgccggcg
      421 cggtctggat gcccatcccg ccctgccccg cgcaccagaa gaagccgggc tcctgcgggt
      481 cgaagccgag cttcagaagc cggtcggggg caaaggtccg gaggcccgcc cacttgcgct
      541 cgaccgcctc gaccggccag tcgaccgcgc gctcgaagcg gtcgatcgcg gtcgcgacgt
      601 cgatctcctc gggcgccgcg tcgcacgggt cgaccggcgt ctcgtcgagc gggctgaccc
      661 agacgctgcg ctccccctcg cccttgaaat agaagctggc gtggaggtcg gtgaccaggg
      721 gcatgtccct gaggccgcag cgcccgatgc gcagctggac gaccgtccgg cgcatcggct
      781 ggagcccgat cggagccacc ccgcagcgcg ccgcgacgtc gtcggcccag gcgcccgcgg
      841 cattgaccag caggcccgcc cgaaccggcc ccgccgcggt ctcgacctgc cacccgtcgc
      901 ccgagcgctt cgccgaaagc agagcggcgt cggtccgcac ctcgccgccc tggcggcgca
      961 ccgcggcgag gcaggcggag tggaacgcgg cgacctcgat gtcggcgcag ctcggctcgt
     1021 accaggcgcc ggtccattgc tcgcgaagcc cgggcaccat cgcgtcgagc tcggcccggt
     1081 cgagccacac cagggcatgc tcgccgacca gatcctcggc gccctcgacc gtgtcgccgt
     1141 gggcgccggt caggtgcagc gtgccgcggc ggcggagcag gggcgtcgga gtccccggcc
     1201 agcgctcgtc gaacatcggc ttggaggcga tgctcagccg ccgcgccggc gacaagccga
     1261 ggctcgcgac ccagaaggcg gccgagcgcc cggtcgaatg gcggccgcac aaatcctccg
     1321 attcgatcag catgattcgc gcgcgtccgg ccaggcgggc gccgaggctg gccccggcga
     1381 tcccgccgcc gacgatcaga atgtcgcaaa cattgtccgg cacgcggttc gaatccccga
     1441 cgaagttttg ccacggttac gaattggaaa gccgcaccgt ctaggggtcg ggcatgatca
     1501 acgccggatt caccgaattg ctgcttgggg tcctggcgat cctgctgctg gtcgcggccg
     1561 tcgtcgacgt acgtaccttc accatttcca actcgctcaa cctcgccatc gcgctgctcg
     1621 cgccgctctt ctggctgtcg atcgctctgc cgctgtggcc gaacgtggcg atccagctgg
     1681 cggtcggcgc cggggtcttc gcactgctcg ccggcgcctt ttacgccggc atgatgggcg
     1741 gcggcgacgt caagctggcg gcggccctgg ccttgtggtt ctcgcccgcc tcgacggtga
     1801 aattcctggt cctgatgtcg ctggccgggg gcgtcctgac gcttgcgatg gtcgctcacc
     1861 accggatgcg cggcaaggcc gggcggcccg aaattccgta tggcgtggcc atcgcttttg
     1921 gcggcctcgc cattctcacc caacggtttc ttaaccaatt tgcctgatgt gtcacaacat
     1981 cgacggggcc ctgtagggga ggcgatcgcg ccatgaacgt gaagaaaatc gcgctgctgt
     2041 ttggagcgct ggtcattgcg gttcttaccg cggtcatggc caagaacatg tttgccgggg
     2101 ctggagcgca gcagg
//
LOCUS       DASTZO010000189         3981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_667623,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000189 DASTZO010000000
VERSION     DASTZO010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3981
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_667623"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VFU20_05250"
     CDS             complement(<1..518)
                     /locus_tag="VFU20_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HEU5285902.1"
                     /translation="MRHHRQRKPRPPLDRARLDELALAYVGRFATTRARLRAYLARKV
                     RERGWDDGPPPDVEAIAERFAAQGYVDDAAFALAKSRSLTGRGYGARRVEQSLRVAGV
                     EEEDSAPARDHARADCVQAAVRFAERRRLGPFASEPADRKGRERALAAMIRAGHGFGL
                     ARAILDLPPGAD"
     gene            656..2413
                     /locus_tag="VFU20_05255"
     CDS             656..2413
                     /locus_tag="VFU20_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HEU5285903.1"
                     /translation="MEPGHARQGLAPPGATPTTDSQKRRIADFATLGEALDYAALGRR
                     GLNFHDARGALERSYFYAQLRDDALANAGRFLALGLRPGDRLALIAETGPEFAAAFFG
                     AVYAGLWPVPLPLPTSFGGREAYVDQLKVQLQSCDPALFLYPAEIALFAGDAAAQLGL
                     EAREWETLGDIDAAAAELPQPAADDIAYLQYSSGSTRFPHGVAVTHRALLDNLRAHGL
                     GLQVADTDRVISWLPWYHDMGLVGCLLSPIANQLSVDYLKTEDFARRPLAWLDLITRN
                     PGTTLSYSPTFGYDICARRMSSQTRAADRFDLSRWRIAGNGADMIRPDVMQAFVDCFA
                     EAGFAASAFCPSYGLAEATLAVSLMPPGEGIRLELVEETELSGCSADRERPRRFRAIV
                     NCGKPVTGMEVEIRGPDGTPLPDRGIGKLHVRGTSVMAGYFRDPESTAACLGPDGWLD
                     TGDMGYLSGGYIFIVGRAKDMIIINGRNHWPQDIEWAVEQLPGFKAGDIAAFAITGSS
                     GEETPAVLVHCRVSDPGERGRLRDDIRERVRAITGITPVVELVPPRTLPRTSSGKLSR
                     TKARNLYLSGEIQPYDIAA"
     gene            complement(2555..3481)
                     /locus_tag="VFU20_05260"
     CDS             complement(2555..3481)
                     /locus_tag="VFU20_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285904.1"
                     /translation="MLHDVDYVLGKLEWMRAQRIWPNGLRYLWTDAFGLVLYLSLHAE
                     TGDRRYLDEAEHLAAEVDRVLGRPRGLRIGEAADRDGQYFHYLAMWLFALARLGEHKP
                     EYRARGIAIARDIHSAFVVPGVGVLWKMTEDLSGPYPGYGLGALDAFDGYVSYRLLGE
                     DALVPEIAEMKALIERQYRSLDVDQDLGLGMMLWLAHFFPDEAWAKEQSARALAALDR
                     LWVDPPGFFGRASYAPEVRIAFANYGVSVGLQAAGRWPERVDKLNAYFETYRSGDEYD
                     REAITHVMACNSHLPGLLIKDRSLVGEVHTES"
     gene            3578..>3981
                     /locus_tag="VFU20_05265"
     CDS             3578..>3981
                     /locus_tag="VFU20_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5285905.1"
                     /translation="MQVFDTVLEAIGDTPLIRLRRASEETGCTILGKCEFLNPGQSVK
                     DRAALFIIQDALRRGAVRPGGVVVDGTAGNTGIGLALVGNALGLRTVIVIPDTQSREK
                     KDTLRALGVDLVEVPAVPYKNPNNYVKVSGRMA"
BASE COUNT          610 a         1446 c         1343 g          582 t
ORIGIN      
        1 atgtcggccc cgggcggcag gtcgaggatc gcccgggcga ggccaaagcc gtgcccggcg
       61 cggatcatcg ccgccagcgc gcgctcgcgc cccttgcggt ccgccggctc gctcgcgaac
      121 gggccgagcc ggcggcgctc ggcgaagcgc accgcggcct ggacgcaatc ggcccgggca
      181 tggtcgcggg cgggcgccga atcctcctcc tcgactccgg cgacccgcag cgactgctcg
      241 acccgccggg cgccgtagcc gcggccggtc agcgaccgcg acttggccag cgcgaaagcg
      301 gcgtcgtcga catagccctg ggccgcgaag cgctcggcga tcgcctcgac gtcgggcggc
      361 gggccgtcgt cccaaccgcg ctcgcggacc ttgcgggcga gataggccct cagccgcgcc
      421 cgggtggtcg cgaaccggcc gacataggcg agcgccagct cgtcgagcct ggcccggtcg
      481 agcggcgggc gcggcttgcg ttggcggtgg tgacgcatgg gccttttttg tgccacactc
      541 ccgaaccatc acaaagcgtg gccggattag cgccacccgt tttgaaaggc cgctacaggc
      601 agccagacga ccgtctcgat gaggaaggaa ccgagtgctc gaccgtaagg ccacgatcga
      661 acccggccat gcccgccagg gccttgctcc tccgggcgcc accccgacga ccgacagcca
      721 gaagcggcgc atcgccgact tcgcgacgct cggcgaggcg ctcgactatg cggcgctcgg
      781 ccgccgcggg ctgaacttcc acgacgcgcg cggggcgctc gagcgcagct atttctatgc
      841 gcagctgcgg gacgatgcgc tggccaacgc cggccgtttc ctcgccctcg ggctcaggcc
      901 cggcgaccgg ctcgcgctga tcgccgagac cgggccggaa ttcgccgccg ccttcttcgg
      961 cgcggtctat gccggcctgt ggccggtgcc gctgccgctg ccgaccagct tcggcggccg
     1021 cgaagcctat gtcgaccagt tgaaggtcca gctgcaaagc tgcgacccgg ctttgttcct
     1081 ttacccggcc gaaatcgctt tgttcgccgg cgacgccgcc gcccagctcg gcctcgaggc
     1141 gcgcgaatgg gagacgctcg gcgacatcga tgccgccgcc gccgagcttc cccagcccgc
     1201 cgccgacgac atcgcctatc tgcaatattc gagcggctcg acccgctttc cgcatggggt
     1261 cgccgtcact caccgggcgc tgctcgacaa tctgcgcgcg cacggcctcg gcctccaggt
     1321 cgccgacacc gaccgggtga tctcctggct gccctggtac cacgacatgg gcctggtcgg
     1381 ctgcctgctg tcgccgatcg ccaaccaatt gtcggtcgac tatctcaaga ccgaggattt
     1441 cgcgcgccgg cccctggcct ggctcgacct catcacccgc aacccgggca ccacgctcag
     1501 ttactcgccg accttcggct acgacatctg cgcccggcgg atgtcgtcgc agacccgcgc
     1561 cgccgaccgc ttcgacctgt cgcgctggcg gattgccggc aacggcgccg acatgatccg
     1621 ccccgatgtg atgcaggcgt tcgtcgactg cttcgccgaa gcgggcttcg ccgccagcgc
     1681 cttctgcccg agctacggcc tcgccgaagc gactctcgcc gtgtcgctga tgccgccggg
     1741 cgagggcatc cggctcgagc ttgtcgagga gaccgagctg tcgggctgca gcgccgaccg
     1801 cgagcggccg cgccggttcc gggcgatcgt caattgcggc aagccggtga ccgggatgga
     1861 ggtcgagatc cgcgggcccg acggaacgcc gctgcccgac cgcggcatcg gcaagctcca
     1921 cgtgcgaggc accagcgtca tggccggcta tttccgcgac ccggaatcga ccgccgcctg
     1981 cctcggtccc gacgggtggc tcgacaccgg cgacatgggc tatctgtcgg gcggctacat
     2041 cttcatcgtc ggccgcgcca aggacatgat catcatcaac ggccgcaacc actggccgca
     2101 ggacatcgaa tgggcggtcg agcaattgcc cgggttcaag gccggcgaca tcgccgcctt
     2161 cgccatcacc ggctcgtcgg gcgaggaaac cccggcggtg ctggtccatt gccgggtgtc
     2221 cgaccccggc gagcgcggcc gcctgcgcga cgacatccgc gagcgggtcc gggcgatcac
     2281 cggcatcacc ccggtcgtcg agctcgtgcc gccgcgcact ttgccgcgca ccagctcggg
     2341 caagctgtcg cgcaccaagg cccgcaacct ctatctgtcc ggcgaaatcc agccctacga
     2401 tattgccgcc tgagccgccg agcgcagcga agcgattgcg cagcgatcgt tagcagcgcg
     2461 tagagccgac gaaggccgga cggcctgcgc cgcgaagacg gcgacaggac cgacgtcacg
     2521 ggattcattc ccgtgacggc ccgtgtgttg acgatcagct ctccgtatga acctcgccga
     2581 ccaggctgcg gtccttgatc agcagcccgg gcaggtgcga attgcacgcc atcacatggg
     2641 tgatcgcctc gcggtcatat tcgtcgccgg accgataggt ttcgaaatag gcgttgagct
     2701 tgtcgacccg ctccggccat cggccggcgg cctgcaggcc gaccgagact ccgtaattgg
     2761 cgaaggcgat ccgcacctcc ggcgcgtagc tggcgcggcc gaagaagccg ggcgggtcga
     2821 cccacaagcg gtcgagcgct gccagcgccc gcgcgctttg ctccttcgcc cacgcctcgt
     2881 ccgggaagaa atgggcgagc cacagcatca ttccgaggcc gaggtcctgg tcgacgtcca
     2941 atgaccggta ctggcgctcg atcagcgcct tcatctcggc gatctcgggc acgagggcgt
     3001 cctcgccgag cagccggtac gacacatagc cgtcgaacgc gtcgagcgcg ccgaggccgt
     3061 agccggggta cgggccgctc aaatcctccg tcattttcca cagcacgcca actccgggga
     3121 cgacgaacgc cgaatggatg tcgcgggcaa tggcgatgcc gcgggcgcgg tactccggct
     3181 tgtgctcgcc gagcctggcg agtgcgaaca accacatcgc gagatagtgg aaatactgcc
     3241 cgtcgcggtc cgccgcctcg ccgatccgga gcccgcgcgg ccggccgagc acgcggtcga
     3301 cctcggcggc gagatgctcg gcctcgtcga ggtagcgccg atcgccggtt tccgcgtgga
     3361 gcgacaggta aaggacgaga ccgaacgcgt cggtccacag gtagcgcagc ccgttcggcc
     3421 agatgcgctg cgcgcgcatc cactcgagct tgcccagcac ataatcgacg tcgtgaagca
     3481 tcggccggcc catcccccaa cgaaccggcc gattgtcggc tccatggtcg cgcgcgccgc
     3541 cgtgcggact cgcgccgccg gtgctaaggg gcggcccatg caggtcttcg acacggtgct
     3601 cgaggcgatc ggcgacacgc cgctgatcag gctccggcgc gcgtcggagg agaccggctg
     3661 cacgatcctc ggcaagtgcg agttcctgaa ccccggccag tcggtcaagg accgggcggc
     3721 cttgttcatc atccaggacg cgcttcgccg cggcgcggtg cggcccggcg gggtggtggt
     3781 cgacggcacc gcgggcaaca ccggcatcgg actcgccctg gtcggcaacg cgctcggcct
     3841 caggaccgtg atcgtcattc ccgacaccca gagccgggag aagaaggaca cgctgcgggc
     3901 gctcggggtc gacctcgtcg aggtgccggc ggtgccgtac aagaacccga acaattacgt
     3961 gaaggtctcg gggcggatgg c
//
LOCUS       DASTZO010000190         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_93227,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000190 DASTZO010000000
VERSION     DASTZO010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93227"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1781
                     /locus_tag="VFU20_05270"
     CDS             <1..1781
                     /locus_tag="VFU20_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEU5285906.1"
                     /translation="AEQAAAAPAAAVPAVPAKNSELAAFFEEVDLAELALSPIAKSYR
                     AIKDADYGKWDQFTDEHALARRELGRRHVAELQRRFDRAALSPDDRLSYDLFLYRDAR
                     TESIFPYRDYGYVFDQMNGAQSDGPAFLINIHKVASVADAEAYVSRLREMARYLDQSI
                     EEARERQTLGVLPPKWVFPYVIADSKNVIRGAPFGTGADSALWADLKSKVGKLGADEA
                     TKQRLLDSARAALLEDVRPAYRRVIALMQEQQKVAGTDDGIWRFPTGAAQYQALLKYY
                     TTVDLTPDQVHELGLAQVARIHGEMEAIKQTVGFNGTLQQFFAHVRDDKQFYYPNNAA
                     GKQRYLDETEKALQQTMAAVPRFFGRIPKSPLLVKPVEPFREKSAGKAFYQDPPPDGS
                     RPGTYYVNLYDMNDMPATEVEALFCHEGIPGHHLQGALLSELGKGEVPPFRQFSGYTA
                     TDEGWGLYSEKLCKEMGLYQDPYRDFGRLQLELHRAIRLVVDSGIHHKRWSREQAIRY
                     VEDNSADAKGGIVKAIERYIVYPGQATAYMVGKLKIEELRRKAEAALGPKFDIRGFHD
                     TILLAGSMPLGMLESRVDEWIARQRS"
     gene            1833..2375
                     /locus_tag="VFU20_05275"
     CDS             1833..2375
                     /locus_tag="VFU20_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU5285907.1"
                     /translation="MRLTHLADYAVVMMTAAARRGALDSGKRPARVSAAELAQDTGVP
                     LPTAQKLMSQLAGTGLLTSVRGAGGGFALARPATDISLADIIEAVEGPIAMTMCSGSD
                     ELSECALDAHCRVKPHMGIVSNAVRGALGAVSLESLVQQPPAVRPEPVEGLSFSSSHT
                     KERQGFDKLSPNGVGVSSAS"
     gene            2372..>2785
                     /locus_tag="VFU20_05280"
     CDS             2372..>2785
                     /locus_tag="VFU20_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU5285908.1"
                     /translation="MTDTLRNREVKEAVARDYEWGFVSDVEQEFAPKGLSEDTVRFIS
                     GKKNEPEWMLEWRLKAYRAWLTMEEVSWAKLDLPVIDYQDAYYYAEPKKRPKLASLDE
                     LDPEIRRTYEKLGIPIEEQKVLAGVEGARKVAVDAV"
BASE COUNT          500 a          926 c          945 g          414 t
ORIGIN      
        1 cggccgagca agcggccgcc gctccggccg ccgcggtccc ggcggtcccg gccaagaact
       61 ccgagctcgc cgctttcttc gaggaggtcg acctggccga gctggcgctg tcgccgatcg
      121 ccaagtcgta ccgggcgatc aaggatgccg attacggcaa gtgggaccag ttcaccgacg
      181 agcatgcgct cgctcggcgc gagctcggcc ggcgccatgt cgccgagctg cagcggcgct
      241 tcgaccgggc ggcgctgtcg cccgacgacc ggctcagcta cgacctgttc ctgtatcgcg
      301 acgcccggac cgaatcgatc ttcccgtacc gcgactacgg ctacgtgttc gaccagatga
      361 acggcgccca gagcgacggg ccggccttcc tgatcaatat ccacaaggtg gccagcgtcg
      421 ccgacgccga ggcctatgtg tcgcggctgc gcgagatggc ccgctatctc gaccagtcga
      481 tcgaggaggc gcgcgagcgc cagacgctgg gcgtgctgcc gcccaagtgg gtgttcccct
      541 acgtgatcgc cgattcgaaa aacgtgatcc gcggcgcccc gttcgggacc ggcgccgaca
      601 gcgcgctgtg ggcggatttg aagtccaagg tcggcaagct cggcgccgac gaggcgacca
      661 agcagcggct gctcgattcg gccagggcgg cgctgctcga ggacgtccgc ccggcctacc
      721 gccgggtgat cgcgctgatg caggagcagc agaaagttgc cggcaccgac gacggcatct
      781 ggcgattccc gaccggcgcg gcgcaatatc aggcgctgct caaatattac acgacggtcg
      841 atttgacgcc cgaccaggtg cacgagctcg ggctcgcgca ggtcgcccga atccacggcg
      901 agatggaggc gatcaagcag acggtcggct tcaatgggac gctgcagcaa ttcttcgcgc
      961 acgtgcgcga cgacaagcaa ttctattacc cgaacaatgc cgccggaaag cagcgctacc
     1021 tcgacgagac cgagaaggcg ctgcagcaga cgatggccgc ggtgccgcgc tttttcggcc
     1081 ggattcccaa gtcgccgctg ctggtgaagc cggtcgagcc gttccgagag aagagcgccg
     1141 gcaaggcctt ttaccaggac ccgccgcccg acggctcgcg gcccggaacc tattacgtca
     1201 acctctacga catgaacgac atgccggcga ccgaggtcga ggccctgttc tgccacgagg
     1261 gcatccccgg ccaccatctg cagggcgcgc tgctgtccga gctcggcaag ggcgaggtgc
     1321 cgccgttccg ccaattctcc ggctacaccg cgaccgacga gggctggggc ctctattcgg
     1381 agaaattgtg caaggagatg gggctctacc aggatccgta ccgcgacttc ggccggctgc
     1441 agctcgaatt gcaccgcgcg atccggctgg tggtcgacag cggcatccac cacaagcgct
     1501 ggagccgcga gcaggcgatt cgctacgtcg aggacaacag cgccgacgcg aagggcggaa
     1561 tcgtcaaggc gatcgagcgc tacatcgtct atccgggcca ggcgaccgcc tacatggtcg
     1621 gcaagctgaa gatcgaggaa ttgcgccgca aggcggaggc cgcgctgggg ccgaagttcg
     1681 acatccgcgg cttccacgac acgatcctgc tcgccggatc gatgccgctc ggaatgctcg
     1741 agagccgtgt cgacgagtgg atcgcgcgcc agcggagttg aagtttcggg cgtctcggcc
     1801 taaatcggac tagaaagctc cgaattaacg ccatgcgcct gacccacctt gccgactatg
     1861 cggtcgtgat gatgaccgcg gccgcccgcc gcggggccct tgattcgggc aagaggccgg
     1921 cgcgcgtgtc cgcggccgag ctcgcgcagg acaccggggt gccgctgccg accgcgcaga
     1981 agttgatgag ccagctcgcc gggaccggcc tgctgaccag cgtccgcggc gccggcggcg
     2041 gcttcgcgct cgcccggccg gcgaccgaca tcagcctcgc cgacatcatc gaggcggtcg
     2101 aggggccgat cgccatgacc atgtgcagcg gcagcgacga gctgtcggaa tgcgcgctgg
     2161 acgcgcattg ccgggtcaag ccgcacatgg gcatcgtcag caacgcggtg cgcggcgcgc
     2221 tgggcgcggt cagcctggaa agcctggtcc agcaaccccc agccgttcgc cctgagcctg
     2281 tcgaagggct gtccttttct tcctcgcata ccaaagaacg gcagggcttc gacaagctca
     2341 gcccgaacgg cgttggcgtg agttcggcgt catgaccgac acgttgcgca accgcgaggt
     2401 caaggaggcc gtcgcccgcg attacgagtg gggcttcgtc tccgatgtcg agcaggagtt
     2461 cgcccccaag ggcctcagcg aggacaccgt ccgcttcatc tcgggcaaga aaaatgagcc
     2521 cgagtggatg ctcgagtggc ggttgaaggc ctaccgcgcc tggctgacga tggaggaggt
     2581 gagctgggcc aagctcgacc tgccggtgat cgactaccag gacgcatatt attacgccga
     2641 gcccaagaag cggcccaagc tcgcgtcgct ggacgagctc gaccccgaga tccgccgcac
     2701 ctacgagaag ctcgggatcc cgatcgagga gcagaaggtg ctcgccgggg tcgagggcgc
     2761 gcgcaaggtc gcggtcgacg cggtg
//
LOCUS       DASTZO010000191         4372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_38100,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000191 DASTZO010000000
VERSION     DASTZO010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38100"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="VFU20_05285"
     CDS             <1..636
                     /locus_tag="VFU20_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5285909.1"
                     /translation="LEQHHYIVRERDRPTYSCLMLSGFSVRHKIVGGGYRQIVAIHMK
                     GEMVDLQNSLLGVADHSVQMLTSGQVALIPRKEITRLAFERPAVGKALWFDTLVDASI
                     FREWIANVGRRDARTRIAHVLCEFALRLKLAGLGEQNGYQLPMTQEQLGDATGLTSVH
                     VNRTLKSLEADGLIERLSPRSITIGDWKKLSEVGDFDSNYLHLREGEPALA"
     gene            686..770
                     /locus_tag="VFU20_05290"
     tRNA            686..770
                     /locus_tag="VFU20_05290"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:719..721,aa:Leu,seq:caa)
     gene            822..2063
                     /locus_tag="VFU20_05295"
     CDS             822..2063
                     /locus_tag="VFU20_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5285910.1"
                     /translation="MDARTSKKTAPETGDEARALAEGITIKRDRLLASLTLIGGAGLL
                     IALPFALRAGAEFFLPVTAALVIAIALVPMLEWFERRGVPSRIAAFLCVLIFLVMAIF
                     AIGSIVLPAIDWVALLPERLPKVRAALDPLLDLYKAFDRFIERILSQIVIAPEAGRTV
                     RIETPNSLSGLLTTSAPHILIQLFFALLVIFFFLAGWTAMRKKTIVSRGSFEGALTTA
                     RVIQQVVDATSTYIGTITVINVTLGALTALILWQLGMDSPIMWGGIVAVLNYIPYLGP
                     IASAGLLFIGGLMTFPDVWSAMLPPAIFVGLHLVEANIITPMVVGKKLTINPLAILIA
                     LSFWAWVWGTTGALLAVPLLIIMKTVFAAAGTPDIAGFLFEEGTLTHIGEAEEQDEDE
                     EPALGDRPAGSGSVDRVGGVT"
     gene            2084..2160
                     /locus_tag="VFU20_05300"
     tRNA            2084..2160
                     /locus_tag="VFU20_05300"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2118..2120,aa:Asp,seq:gtc)
     gene            complement(2204..3295)
                     /locus_tag="VFU20_05305"
     CDS             complement(2204..3295)
                     /locus_tag="VFU20_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEU5285911.1"
                     /translation="MTQALILSAARLWRAHPRETAVVGTLGLAAAFALAGAAWSNASL
                     SGLGSDSARLEKAPPAPPPLLVRELAPEDALAINRAIPIAASPNPAARPFALRGADEA
                     TRARALECLTSAVYYEAGNESAEGQKAVAQVVLNRVRHPAFPASVCDVVYQGSTRPTG
                     CQFTFTCDGSLARRPNADGWARARKVAAAALAGAVFAPAGYATHYHANYVVPYWASSL
                     AKNAIVGAHIFYRWAGGWGRPAAFGKRYSGREPSTQALKAAAVAAEAAYAAQEAAAAT
                     AAEVVAAIPGAELKTPEEGRLAIRFNLAARKAADEAPHRDYVEKFEASDNLRWSLSGG
                     AVAADEKPLGKASPQVGTAGGGGAGAPQL"
     gene            complement(3333..3572)
                     /locus_tag="VFU20_05310"
     CDS             complement(3333..3572)
                     /locus_tag="VFU20_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HEU5285912.1"
                     /translation="MTPATGPSWRKGAGIAAIVLLILIWAALVASLAPMIGNWPILVQ
                     GAFYLVTGIVWIIPLKPLLRWAETGRWRRPTKSGD"
     gene            complement(3569..4159)
                     /locus_tag="VFU20_05315"
     CDS             complement(3569..4159)
                     /locus_tag="VFU20_05315"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEU5285913.1"
                     /translation="MVPSPPFPALAKAALRSAALDARKAYVAALSDADRTLLERRLAE
                     QLTAVCANATVVGGYSPLGSEISPLLAMEEARAVGAIVAFPAFDDPARPFRFLAGDPS
                     AGGPFGILQPRLTAPPVEPDVILVPLIAVDRRGTRLGRGKGHYDRVLAPYRRKGAQLI
                     GVGWTLQRIAEEIPRDPWDVPLDSFASPEGLERLDP"
BASE COUNT          653 a         1505 c         1524 g          690 t
ORIGIN      
        1 ctcgagcagc accattacat cgtccgcgag cgcgaccggc cgacatactc ctgcctgatg
       61 ctttcgggtt tctcggtccg gcacaagatc gtcggcggag gctaccggca gatcgtcgcc
      121 atccacatga agggcgagat ggtcgacctc cagaattcgc tgctcggggt tgccgaccac
      181 agcgtccaga tgcttacctc cggccaggtc gcgctgatcc cgcgcaagga aatcacccgc
      241 ctcgcgttcg agcggccggc ggtcggcaag gcgctgtggt tcgacacgct ggtcgacgcc
      301 tcgatcttcc gcgagtggat cgccaacgtc gggcggcgcg acgcgcggac ccgcatcgcc
      361 cacgtgctgt gcgagtttgc gctgcggctc aaactggccg ggctcggcga gcagaacggc
      421 taccagctgc cgatgaccca ggagcagctc ggcgacgcga ccgggcttac gtcggtccac
      481 gtcaatcgga ccctgaaatc gctcgaggcg gacgggctga tcgaacggct gagcccgcgc
      541 tcgatcacca tcggcgactg gaagaagctg tccgaggtcg gcgatttcga cagcaattac
      601 ctgcacctgc gcgaaggcga gccggcgctg gcctgaccac cgccgccatt gccgcgcgcg
      661 gcgctcatcg ctagctaaag cgcgggcccc tgtggcggaa tggtagacgc gctcgactca
      721 aaatcgagtt cctaaccgga gtgtccgttc gagtcggacc aggggcacca tcgcttcaaa
      781 agcacatggc gaagccgcgc ttgcgccgat atggtgcgcc catggacgcg cggaccagca
      841 agaagacggc gccggagacg ggcgacgagg cgcgcgcgct ggccgagggc atcaccatca
      901 agcgcgaccg gctgctcgcc tcgctgaccc tgatcggcgg cgcggggctg ctgatcgctc
      961 tgccgttcgc gcttcgcgcc ggcgccgaat tcttccttcc ggtcaccgcc gcgctggtga
     1021 tcgcgatcgc cctggtgccg atgctcgaat ggttcgagcg gcgcggggtc ccctcgcgca
     1081 tcgccgcctt cctgtgcgtg ctgatcttcc tggtcatggc gatcttcgcg atcggctcga
     1141 tcgtccttcc ggcgatcgac tgggtggcgc tgttgcccga acggctgccc aaggtgcgcg
     1201 cggcgctcga tcccttgctc gacctctaca aggcgttcga ccggttcatc gagcggatcc
     1261 tgtcgcagat cgtcatcgct cccgaggccg ggcggacggt gcgcatcgaa acccccaatt
     1321 cgctgtccgg gctactgacc acctcggcgc cgcacatcct gatccagttg ttcttcgcgc
     1381 tgctggtgat cttcttcttc ctcgccgggt ggacggcgat gcgcaagaag acgatcgtca
     1441 gccgcggcag cttcgaaggc gccctgacca ccgcgcgcgt gatccagcag gtggtcgacg
     1501 cgacctcgac ctacatcggc acgatcaccg tcatcaacgt gacgctgggg gccctgacag
     1561 cgctgatcct gtggcaattg ggtatggact cgccgatcat gtggggcggc atcgtcgcgg
     1621 tgctcaacta catcccgtat ctggggccga tcgcttcggc cgggctgttg ttcatcggcg
     1681 ggctgatgac cttccccgac gtctggtcgg cgatgcttcc gccggcgatc ttcgtcgggc
     1741 tccatctcgt cgaggcgaac atcatcacgc cgatggtggt cggcaagaag ctgaccatca
     1801 acccgctggc gatcctgatc gcgctctcct tctgggcgtg ggtgtggggg accaccgggg
     1861 cgctgctggc ggtgccgctg ctgatcatca tgaagaccgt gttcgccgcc gccggcacac
     1921 cggacatcgc gggattcctg ttcgaggaag gcactctcac ccacatcggc gaggccgagg
     1981 aacaggacga ggacgaggag ccggcgctcg gcgaccgccc ggccggcagc gggtcggttg
     2041 acagggttgg gggggtcacc taactgcccg cgacgcaaac cgcgcgggtg tagctcagtt
     2101 ggttagagcg ccggcctgtc acgccggagg tcgcgggttc aagtcccgtc actcgcgcca
     2161 tttccgcttc gatgtcggct ggccgcccga cccgtatccg cgcctagagc tgcggcgcgc
     2221 ccgctccgcc gccgcccgcc gttccgacct ggggcgacgc cttgccgagc ggcttctcgt
     2281 cggcggcgac cgccccgccg ctcagcgacc agcgcagatt gtccgaggct tcgaacttct
     2341 cgacataatc gcggtgcggg gcctcgtcgg ccgcctttcg cgcggcgagg ttgaagcgga
     2401 tcgcgaggcg cccttcctcg ggcgtcttca attcggcgcc ggggatcgcg gcgacgacct
     2461 cggcggccgt ggccgcggcg gcctcctgcg ccgcatacgc cgcctcggcg gcgaccgccg
     2521 cggccttcag cgcctgggtg ctcggctcgc ggccggaata acgcttgccg aacgcggccg
     2581 gacggcccca gccgcccgcc cagcgataga agatgtgcgc cccgacgatc gcgttcttgg
     2641 ccaggctcga cgcccaatag ggcaccacgt aattggcatg gtaatgggtg gcgtagccgg
     2701 ccggagcgaa caccgctccg gcgagcgccg ccgcggcgac cttgcgcgcc cgggcccagc
     2761 cgtcggcgtt cggccggcgc gccagcgagc cgtcgcaggt gaaggtgaac tggcagccgg
     2821 tcgggcgtgt cgagccctgg tagacgacgt cgcacacgct tgccgggaag gccgggtggc
     2881 ggacccggtt gaggacgacc tgcgcgaccg ccttctggcc ctcggcgctc tcgttgccgg
     2941 cctcgtaata gacggcgctg gtcaggcact cgagcgcccg cgcccgggtt gcctcgtcgg
     3001 cgccgcgcaa ggcgaacggc cgggcggccg ggttgggcga cgcggcgatc gggatcgccc
     3061 ggttgatcgc cagcgcgtcc tcgggcgcga gctcgcggac caggagcggc ggcggcgccg
     3121 gcggcgcttt ttcgagccgc gccgaatcgc tgccgaggcc ggacaggctc gcgttcgacc
     3181 aggccgcccc cgcgagcgcg aaggcggcgg caaggccaag cgtgccgacg accgcggtct
     3241 cgcgcgggtg agcccgccac agccgggcgg cggaaaggat cagggcctgg gtcatgacgg
     3301 cgtcggtgcc atagtcgaat aatacacttg gttcaatcgc ccgatttggt cggcctgcgc
     3361 cagcggccgg tctccgccca ccgcagcaac ggtttcagcg ggataatcca gactattccc
     3421 gtcacgaggt agaacgcgcc ctgaacgaga atgggccaat tgccaatcat cggcgccagg
     3481 cttgcgacga gtgctgccca aatgaggatc agcagcacga tggccgcgat gcccgcgccc
     3541 ttgcgccagg atggaccggt cgcgggcgtc atgggtcgag tcgctcgagg ccctcgggcg
     3601 aggcgaacga gtcgagcggc acgtcccagg ggtcgcgcgg gatctcctcg gcgatccgct
     3661 gcagcgtcca gccgaccccg atcagctgtg cgcccttgcg ccggtagggc gcgagcaccc
     3721 ggtcgtaatg gcccttgccg cggccgagcc gggtgccgcg ccggtcgacc gcgatcagcg
     3781 gcaccaggat gacgtcgggc tcgaccggcg gggcggtcag ccgcggctgc aggatcccga
     3841 acgggccgcc ggccgacggg tcgccggcga ggaagcggaa cggcctggcc gggtcgtcga
     3901 acgccgggaa ggcgacgatc gcgccgaccg cccgggcttc ttccatcgcc agcagcgggc
     3961 tgatttcgct gcccagcggc gaatagccgc cgaccacggt ggcgttcgcg cagaccgcgg
     4021 tcagctgctc ggcgagccgc cgttcgagca aggtgcggtc ggcgtcgctc aacgctgcga
     4081 cataggcctt gcgcgcatcg agcgcggcgc ttcgaagcgc cgccttggcc agtgcgggga
     4141 agggaggtga cgggaccacc gtggccgttt gccggaaatc ctctgacgcc acaaacgtca
     4201 ggtgggtgcc gtgtgcaccg gaccccaatg gaaacagagt ggcccgggca gggacaactc
     4261 ccgtggatgg taatagcctc agggatatcg aaggctcgta ccgggcagtt cccgtcggcc
     4321 cctatctagg cggtccgggc ctcgctctca agggagtcgg ccagcgcttc ga
//
LOCUS       DASTZO010000192         3857 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_747256,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000192 DASTZO010000000
VERSION     DASTZO010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747256"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="VFU20_05320"
     CDS             <1..456
                     /locus_tag="VFU20_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5285914.1"
                     /translation="VLGYVGAAATKAMTVALVILATANLFVDVRIEHPLFMLGFLLLV
                     AVTFCLFGFIVGIWAKGFEQLQVIPLLVVTPLTFLGGAFYSIDMLAEPWRSITLFNPV
                     VYLINGFRWTFFGQADVPVLASLTATLAFLLACLAAIWWIFRTGYRLKQ"
     gene            complement(446..1111)
                     /locus_tag="VFU20_05325"
     CDS             complement(446..1111)
                     /locus_tag="VFU20_05325"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEU5285915.1"
                     /translation="MAFQLPDLPFAKDALAPHMSAETLEFHHGKHHRAYVEKTNELIG
                     DKALSGASLVEVVRAAKRDGDAKLFNNSAQLWNHSFFWQCLAPAQGQRPQGKLRQMID
                     EGFGGLDGLLDKLADEAAEHFSNGWAWLLLDRGALKIASLHDADTPLVHDGMVPLLTL
                     DVWEHAYYIDYRNDRPKFAGSVLANIVNWEFVAENLDGRGADRADRADQQPVAARESE
                     LTA"
     gene            1256..1825
                     /locus_tag="VFU20_05330"
     CDS             1256..1825
                     /locus_tag="VFU20_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HEU5285916.1"
                     /translation="MGKLFDNLNLVLAIGVGLAIALMAGVHAEILTAEAVLRWLHVFF
                     GITWIGLLYYFNFVQIPVMPTVPSELKPGVSKYIAPKALFYFRWGAAFTVLTGAVLAW
                     VKNYIGEALMLRDGFELIGIGMWLALIMAANVWFVIWPNQKRALGLVEADDARKAASA
                     RTAMVASRTNVILSLPMLYAMTTYQTLGS"
     gene            complement(1842..2399)
                     /locus_tag="VFU20_05335"
     CDS             complement(1842..2399)
                     /locus_tag="VFU20_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5285917.1"
                     /translation="MKASLLAATTAIAILAAAAPVQGARITIGSTMARACYEAAEALR
                     SDPQSRRICDMALSEEGLSHHDLTGTHVNRGILFMLSGDLDLADRDYDRALELDPNEP
                     EAWLNKGINALKRGDSQVALGFVEKAIGLRTRKPAIAYYMRGIAHEEAGNVRAAYADL
                     QRARMLDPTWPLPAVELQRYRVRTR"
     gene            complement(2483..3532)
                     /gene="pspF"
                     /locus_tag="VFU20_05340"
     CDS             complement(2483..3532)
                     /gene="pspF"
                     /locus_tag="VFU20_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HEU5285918.1"
                     /translation="MERVTQLVGQSLAFLDAVERASRAAPLNRPVLVIGERGTGKELI
                     AERLHHLSPRWSGPLVTMNCAALPENLIEAELFGHEAGSFTGASKTRHGRFEEADGGT
                     LFLDELATLSSPAQDRLLRAVEYGEVTRIGASKPISVDVRIVAATNEHLPRLVEENRF
                     RADLLDRLSFEVVTLPPLRARSGDIPLLADHFGRRMAVELDWPNWPGYSPQVMAQLEA
                     YPWPGNVRELRNAVERAVYRAEDPERPIAEVQFDPFQSPWAASSAPAAVPTGAPAAAG
                     EPAEGAQPQPAAVSPNSAVNDFRSAVAAYERTLLEEALARNRFNQRATAAALGLSYDQ
                     LRHALKRHRLQDSAA"
     gene            3711..>3857
                     /locus_tag="VFU20_05345"
     CDS             3711..>3857
                     /locus_tag="VFU20_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEU5285919.1"
                     /translation="MGIFSRTRDIFAANMTELLDRSEDPARMIRMIILEMEETLVEVR
                     ASAAR"
BASE COUNT          553 a         1267 c         1336 g          701 t
ORIGIN      
        1 gtgctcggct atgtcggggc ggcggcgacc aaggcgatga ccgtcgccct ggtcatcctc
       61 gcgaccgcga acctgttcgt cgacgtccgc atcgagcacc cgctgttcat gctcggcttc
      121 ctgctgctgg tcgcggtgac cttctgcctg ttcggcttca tcgtcggcat ctgggccaag
      181 ggcttcgagc aattgcaggt gatcccgctg ctggtcgtca ccccgctgac cttcctcggc
      241 ggcgccttct actcgatcga catgctggcc gagccgtggc gcagcatcac tctgttcaac
      301 ccggtcgtct atctgatcaa cggcttccgc tggaccttct tcggccaggc cgacgtgccg
      361 gtcctggcca gcctgaccgc gaccctggcc ttcctgctcg cctgcctcgc ggcgatctgg
      421 tggatcttcc ggaccggcta ccggctcaag cagtaagctc gctctcccgg gccgccaccg
      481 gctgctggtc ggcgcggtcg gcgcggtcgg cgccgcgccc gtcgaggttc tccgccacga
      541 actcccagtt gacgatgttg gcgagcacgc tgccggcgaa tttcggccgg tcgttgcgat
      601 agtcgatgta ataggcgtgt tcccacacat cgagcgtgag caagggcacc atgccgtcgt
      661 ggaccagcgg cgtgtcggcg tcgtgcaggg acgcgatctt cagggcgccg cggtcgagca
      721 gcagccacgc ccagccgttg gagaaatgct cggcggcttc gtcggcgagc ttgtcgagca
      781 ggccgtcgag cccgccgaag ccctcgtcga tcatctgccg gagcttgccc tgggggcgct
      841 ggccctgcgc cggcgccagg cattgccaga agaagctgtg gttccacagc tgggcgctgt
      901 tgttgaaaag ctttgcatcg ccgtcgcgct tcgccgcccg gaccacctcg accagcgagg
      961 cgccggacag cgccttgtcg ccgatcagct cgtttgtctt ctcgacgtag gcgcggtggt
     1021 gcttgccgtg gtggaactcg agcgtctcgg ccgacatgtg cggcgccagc gcgtccttgg
     1081 cgaacggcag gtcgggcagc tgaaaggcca tcgcctcctc ctttggttct tggatgctta
     1141 gggggaacga gcggttcccg cggaagctcc gcaagtcgcc gcataagctt gcggttgggc
     1201 gctctctggc cgtagaagcg cggcgctcgc gaccgacgga aaatagggga ccgaaatggg
     1261 caagctgttc gacaatctga acctggtgct ggcgatcggg gtcggcctgg cgatcgccct
     1321 gatggccggc gtccacgccg agatcctgac cgccgaggcg gtgctgcgct ggttgcacgt
     1381 cttcttcggg atcacgtgga tcgggctgct gtactatttc aacttcgtcc agatcccggt
     1441 gatgccgacg gtgccgtccg agctgaagcc cggggtgtcg aaatatatcg ctcccaaggc
     1501 cttgttctat ttccgctggg gcgcggcgtt caccgttctg accggcgcgg tcctcgcctg
     1561 ggtcaagaac tacatcggcg aggcgctgat gctccgcgac ggcttcgagc tgatcgggat
     1621 cggcatgtgg ctggcgctga tcatggcggc caacgtctgg ttcgtgatct ggccgaacca
     1681 gaaacgcgcg ctcggcctgg tcgaggcgga cgatgcccgc aaggcggcct cggcgcggac
     1741 cgcgatggtc gcgtcgcgaa ccaacgtgat tctatcgctg ccgatgctct acgcgatgac
     1801 cacctaccag acgctcggca gctgaccggc gcctggccgg gctagcgggt ccgcacccgg
     1861 tagcgctgca gctccacggc cggcagcggc cacgtcgggt cgagcatccg cgcccgctgc
     1921 aggtccgcat aggcggcgcg gacgttgccg gcttcctcat gggcgatgcc gcgcatgtag
     1981 taggcgatcg cgggcttgcg ggtgcgcagg ccgatcgcct tctcgacgaa cccgagggcg
     2041 acctggctgt cgccgcgctt caacgcgttg atgcccttgt tgagccaggc ctccggctcg
     2101 ttcgggtcga gctcgagcgc tcggtcatag tcccggtcgg ccaggtcgag gtcgcccgac
     2161 agcatgaaca ggatgccgcg gttgacgtgg gtgccggtga ggtcgtggtg ggacagcccc
     2221 tcttcgctca gcgccatgtc gcagatccgc ctggactgcg ggtcggaccg aagagcctcg
     2281 gccgcctcgt aacaggcccg cgccatggtc gagccgatgg tgatccgcgc accctggacg
     2341 ggcgcggcag cggccaggat cgcaatggcg gtggtggccg caaggagcga tgctttcatc
     2401 gaagcctcct tgtcttggac ccgcgcctta taccgcagct ggagcgcggc ttcactcgct
     2461 attcgaccaa cgcactcgga tttcaggcgg cgctgtcctg gagccggtgg cgcttgagcg
     2521 cgtggcgcag ctggtcgtag ctcaggccca gcgccgcggc ggtggcgcgc tggttgaagc
     2581 ggttgcgggc cagcgcttcc tcgagcagcg tccgctcgta ggcggcgacc gccgagcgga
     2641 agtcgttgac cgcactgttg ggggaaacgg ccgcgggctg cggctgcgcc ccttcggccg
     2701 gctcgccggc cgcggccgga gcgcccgtcg gcacggcggc cggggcgctg gatgcggccc
     2761 acggcgactg gaacgggtcg aactggacct cggcgatcgg ccgctcgggg tcctcggcgc
     2821 ggtagacggc gcgctcgacc gcgttgcgca gctcgcggac attgccgggc cacggatagg
     2881 cctcgagctg ggccatcacc tgcggcgaat agccgggcca gttcggccaa tcgagctcga
     2941 ccgccatccg ccgcccgaaa tggtcggcga gcagcggaat gtcgccgctc cgggcgcgca
     3001 gcggcggcaa ggtgacgacc tcgaacgaca ggcggtcgag caggtcggcc cggaagcggt
     3061 tttcctcgac cagccgcggc aggtgctcgt tggtcgcggc gacgatgcgg acgtcgacgc
     3121 tgatcggctt ggaagcgccg atccgggtga cctcgccata ttcgaccgcc cgcagcagcc
     3181 ggtcctgcgc cggcgacgac agggtcgcca gctcgtcgag gaacagggtg ccgccgtcgg
     3241 cctcctcgaa gcggccgtgg cgggtcttgg aggcgccggt gaagctgccg gcctcgtggc
     3301 cgaacagctc ggcctcgatc agattctccg gcagcgccgc gcaattcatc gtcaccaggg
     3361 gtcccgacca gcgcggcgag agatggtgca gccgctcggc gatcagctcc ttgccggtgc
     3421 cgcgctcgcc gatcaccagc accggccggt tgagcggcgc ggcgcggctc gcgcgctcga
     3481 ccgcgtcgag aaacgcaagg ctctgcccga ccagttgagt gacccgctcc atggattcct
     3541 tcaaaaaatc tggccgactg ttggtggtaa agcccatctc ttggcaagat atttcattgg
     3601 tttttcggca gaaaatcgcg gctttttacg gcttttcgct attttctgga tgctggcacg
     3661 cctcttgaat aaggaaaagc gcacagggcc ctgatttgcg gagtgttgga atgggtatct
     3721 tctcccgcac gcgggacatt ttcgcggcga acatgaccga gcttctcgac cgttcggagg
     3781 atccggcgcg gatgatccgg atgatcatcc tcgagatgga ggagactctg gtcgaggtgc
     3841 gcgcctcggc ggcgcgc
//
LOCUS       DASTZO010000193         3495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_848328,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000193 DASTZO010000000
VERSION     DASTZO010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848328"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..847)
                     /gene="dinB"
                     /locus_tag="VFU20_05350"
     CDS             complement(<1..847)
                     /gene="dinB"
                     /locus_tag="VFU20_05350"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU5285920.1"
                     /translation="MQSAEQPLATSPERPVRKIIHVDMDAFYASVEQRDDPSLKGKPV
                     AVGGGHRGVVAAASYEARRYGVRSAMPSVSAKRRCPELIFVKPRFDVYRAVSGQVRDI
                     FADYTDLVEPLSLDEAYLDVTQDKLGFGSARAIAEDIRRRIRAETGLTASAGVSYCKF
                     IAKLASDYRKPDGLCVIPPGRGPDFVATMPVARFHGVGPVTARKMGRLGIRTGADLRT
                     WSLARLEAHFGSSGSWYWRICRGIDEREVRPDRPYKSVSAERTFDDDLRDPERLAAEL
                     TRIAGY"
     gene            879..2084
                     /gene="sucC"
                     /locus_tag="VFU20_05355"
     CDS             879..2084
                     /gene="sucC"
                     /locus_tag="VFU20_05355"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HEU5285921.1"
                     /translation="MNIHEYQAKELLAKFGVPVPAGYPAMSVAEAVAAAGKLPGPLWV
                     VKSQIHAGGRGKGRFKELGPDAKGGVRLARSVDEVRAHAEEMLGKTLVTVQTGPAGKT
                     VQRLYITDGVDIAREFYLALLVDRGSGRIAIVASTEGGVDIEGVAHDTPEKIETLTID
                     PATGLMPHHGRAVAAALGLKGEHAKQAQAVLARLYDAFLGTDASQIEINPLAVTEDGQ
                     LLVLDAKVAFDGNAEFRHPDLEELRDLAEEERQEIEASKYDLAYIKLDGDIGCMVNGA
                     GLAMATMDIIKLNGGVPANFLDVGGGASKEKVTAAFKIILSDPAVKGILVNIFGGIMR
                     CDIIAEGIVAAAREVHLTVPLVVRLEGTNVAQGKDILATSGLPIIPADDLGDAAKKIV
                     AEVRQTVPA"
     gene            2104..2865
                     /locus_tag="VFU20_05360"
     CDS             2104..2865
                     /locus_tag="VFU20_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HEU5285922.1"
                     /translation="MKLLVAVKRVIDFNVKPRVKMDGSGIDLANVKMSMNPFDEIAVE
                     EATRLKEKGVATEVVAVSIGPMKAQETLRTALAMGADRAILVQTPDEIGSEVEPLAVA
                     KILARIAEEEQPGMVILGKQAIDDDSNQTGQMLAALLGWPQGTFASKVEVGDGEVTVA
                     REVDGGAETVKLKLPAVVTTDLRLNEPRYASLPNIMKAKSKPLATRTPDELGVDIARR
                     LETLKVVEPPRRQAGVKVASVDELVDKLKVLGVVK"
     gene            2862..>3495
                     /locus_tag="VFU20_05365"
     CDS             2862..>3495
                     /locus_tag="VFU20_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004206978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HEU5285923.1"
                     /translation="MKVLVLIEHEGGEVRDATLSAVTAAAKLGEVHGLVVGTHVAPVA
                     EATARIAGMTKVHVAEAPHLEHALAETTAPIAARLMADHDAFVAPSTTTGRNIAPRVA
                     ALLDVMQVSDIISVEDEHTFTRPIYAGNAIATVRSTDPKKVITVRGTAFEKAARDGGS
                     AAIEHVDAGADPAISSFVSAETSESERPELTSAKIIVSGGRGFGSSEQFHG"
BASE COUNT          604 a         1184 c         1237 g          470 t
ORIGIN      
        1 cataaccggc aatgcgcgtc agctctgcgg cgaggcgttc gggatcgcgc aaatcgtcat
       61 cgaacgtgcg ctcggcgctg accgacttgt acggccggtc gggccggacc tcgcgctcgt
      121 cgatgccgcg gcagatccgc cagtaccagc tgcccgagct tccgaaatgc gcctcgagcc
      181 gcgccaggct ccaggtccgc aggtcggcgc cggtccggat tccgagccgc cccatcttgc
      241 gcgcggtcac cggcccgact ccgtggaagc gcgccaccgg catggtcgcg acgaagtcgg
      301 ggccgcgccc gggcgggatc acgcagaggc cgtcgggctt gcggtaatcg gaggcgagct
      361 tggcgatgaa cttgcagtag ctgaccccgg ccgaggcggt gagcccggtc tcggcgcgga
      421 tccggcggcg gatgtcctcg gcgatcgccc gagcgctgcc gaagccgagc ttgtcctgcg
      481 tgacgtcgag ataggcctcg tcgagcgaca gcggctcgac caggtcggtg tagtcggcga
      541 agatgtcgcg cacctggccg gagaccgccc ggtagacgtc gaagcgcggc ttgacgaaga
      601 tgagctcggg gcagcggcgc ttggcgctga ccgacggcat cgccgagcgc acgccgtatc
      661 tgcgcgcctc gtaggatgcc gcggcgacga cgccgcgatg gccgccgccg accgccaccg
      721 gtttgccctt gagcgacgga tcgtcgcgct gctcgaccga cgcgtagaag gcgtccatgt
      781 cgacgtggat gatcttgcgg accggccgct ccggcgaggt ggcgaggggc tgctcggcgc
      841 tttgcatggt ggcgccaatc tgattaaggc cgcgcggcat gaacatccac gaatatcagg
      901 ccaaggagct gctcgcgaag ttcggggtgc cggtgcccgc gggctatccg gcgatgagcg
      961 tcgccgaagc ggtcgcggcg gccgggaagc tgcccggccc gctgtgggtg gtgaagtcgc
     1021 agatccacgc cggcggccgc ggcaagggcc gcttcaagga gctcgggccc gacgccaagg
     1081 gcggagtgcg gcttgcccgc tcggtcgacg aggttcgcgc ccacgccgaa gagatgctgg
     1141 gcaagaccct ggtcaccgtc cagaccggcc cggccggcaa gacggtgcag cggctctaca
     1201 tcaccgacgg agtcgacatc gcccgggaat tctatctggc gctgctggtc gaccgcggca
     1261 gcggccggat cgccatcgtc gcctcgaccg agggcggggt cgacatcgag ggcgtcgccc
     1321 acgacacgcc cgagaagatc gagaccctga ccatcgaccc ggcgaccggt ctgatgccgc
     1381 accacggccg cgccgtcgcc gccgcgctgg gcctcaaggg tgagcatgcc aagcaggctc
     1441 aggcggtgct cgccaggctc tacgacgcct tcctcggcac cgacgccagc cagatcgaga
     1501 tcaacccgct ggcggtgacc gaagacggcc aattgctggt gctcgacgcc aaggtcgcgt
     1561 tcgacggcaa cgccgagttc cgccacccgg acctggaaga gctgcgcgac cttgccgaag
     1621 aggagcgaca agagatcgag gcgtcgaaat acgacctcgc ctacatcaag ctcgacggcg
     1681 acatcggctg catggtcaac ggcgccggcc tggcgatggc gaccatggac atcatcaagc
     1741 tgaacggcgg agtcccggcc aatttcctcg acgtcggcgg cggcgcgtcg aaggagaagg
     1801 tcaccgcggc gttcaagatc atcctcagcg atcccgcggt gaagggcatc ctggtcaaca
     1861 tcttcggcgg catcatgcgc tgcgacatca tcgccgaggg catcgtcgcc gcggcccgcg
     1921 aggtgcatct gaccgtgccg ctggtcgtcc ggctcgaggg caccaacgtc gcccagggca
     1981 aggacatcct cgccaccagc ggcctgccga tcatccccgc cgacgacctc ggcgacgcgg
     2041 cgaagaagat cgtcgccgag gtccggcaaa ccgtccccgc ctgattagtc tggagaggca
     2101 agaatgaagc tgctggtcgc ggtcaagcgg gtgatcgatt tcaacgtcaa gccgcgggtc
     2161 aagatggacg gctcgggcat cgacctcgcc aacgtcaaga tgagcatgaa cccgttcgat
     2221 gagatcgccg tcgaggaagc cacccgcctg aaggagaaag gcgtcgcgac cgaggtcgtc
     2281 gcggtgtcga tcgggcccat gaaggcgcag gagaccttgc gcaccgcgct ggcgatgggc
     2341 gccgaccgcg cgatcctcgt ccagaccccg gatgagatcg gctcggaggt cgagccgctc
     2401 gcggtcgcca agatcctcgc caggatcgcc gaggaggagc agccggggat ggtgatcctc
     2461 ggcaagcagg cgatcgacga cgactcgaac cagaccgggc agatgctcgc ggcgctgctc
     2521 ggctggccgc aaggcacgtt cgcctcgaag gtcgaggtcg gcgacggcga ggtcacggtc
     2581 gcgcgcgagg tcgacggcgg ggcggagacg gtgaagctca agctcccggc ggtggtcacc
     2641 accgacctca ggctcaacga gccgcgctac gcgagcctgc ccaacatcat gaaggccaag
     2701 tcgaagccgc tggcgacccg gaccccggac gagctcgggg tcgacatcgc gcggcgcctg
     2761 gaaacgctga aggtggtcga gccgcccagg cgccaggccg gcgtcaaggt ggcgtccgtc
     2821 gacgagctgg tcgacaaatt gaaagtgctg ggggtggtga aatgaaggtg ctggtgctga
     2881 tcgagcacga aggcggcgag gtccgcgacg cgaccctgtc ggcggtcacc gcggcggcca
     2941 agctcggcga ggtccacggg ctggtcgtcg gcacccatgt cgccccggtc gccgaggcca
     3001 cggcgaggat cgccggaatg accaaggtcc atgtcgccga ggcgccgcac ctcgagcatg
     3061 cgctggccga gaccaccgcc ccgatcgcgg ccaggctgat ggcggatcac gacgcgttcg
     3121 tcgccccgtc gaccacgacg ggccgcaaca tcgcgccgcg ggtcgccgcc ctgctcgacg
     3181 tgatgcaggt cagcgacatc atctcggtcg aggacgagca caccttcacc cgcccgatct
     3241 acgccggcaa cgcgatcgcg accgtgcgct cgaccgaccc caagaaggtc atcacggtgc
     3301 gcggcacggc gttcgagaag gccgcgcgcg acggcggcag cgcggcgatc gagcacgtcg
     3361 acgccggcgc cgacccggcc atcagcagct tcgtttccgc cgagaccagc gagagcgagc
     3421 gccccgagct gaccagcgcg aaaatcatcg tctcgggcgg ccgcgggttc ggcagttccg
     3481 agcagttcca cggcc
//
LOCUS       DASTZO010000194         5372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_16451,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000194 DASTZO010000000
VERSION     DASTZO010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16451"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFU20_05370"
     CDS             complement(<1..296)
                     /locus_tag="VFU20_05370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU5285924.1"
                     /translation="MASRNQRMHGWVEGARDRCAVPGCGEPGEYKAPLEPANFNGPGS
                     WRFLCLDHVREHNSAYNYFDGMSPEEISAAQTPYGGWERSVRAFSPTAGATPVW"
     gene            356..643
                     /locus_tag="VFU20_05375"
     CDS             356..643
                     /locus_tag="VFU20_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HEU5285925.1"
                     /translation="MRDASTGPVATEMLRRLNSSLSPSRIELIDDSEQHRGHGGYHPA
                     GESHFTLRIESPAFAGKSRIERQRMVHKALGDLLDERVHALSIRATAPGEA"
     gene            640..1539
                     /locus_tag="VFU20_05380"
     CDS             640..1539
                     /locus_tag="VFU20_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU5285926.1"
                     /translation="MTDNPIAQTIAPVTHDLGAFKVRRALPTRERTMVGPFIFVDEFG
                     PARLPPGQGMDVRPHPHINLATVTYLFDGAIEHRDSLGSRQVIEPGAINLMTAGRGIV
                     HSERSPADSRAAGPSFYGMQTWLALPDGGEEVDPAFDHVPAGGLPLVESGGASARVLM
                     GTLWGASAATPCHSPTIYADIALTVGGSVPIDSEADERALMLVGGEAELDGVPLEPFA
                     LYVLAPGHSARLSSASEARAMLMGGGAFSTPRHVYWNFVSSSRERINQAKDDWQAMRF
                     PLIPGDDQEFIPLPAVPTTVSYP"
     gene            1536..2450
                     /locus_tag="VFU20_05385"
     CDS             1536..2450
                     /locus_tag="VFU20_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5285927.1"
                     /translation="MSEFRRIALATGVTLNVAVAGDPAAPPVVLLHGFPESHRTWRGL
                     APLLQDRFRLVMPDQRGFAGSDRPQGVDAYRTDKVVADLFALADALGVDRFALVGHDW
                     GGAVAWAAATRGDPRLTRLAIVNAPHPVVFQKSLVEDAGQRAASQYINAFRAPGFERL
                     VEAMGYEAFFEKSFGPHVDLARIPEAEKRQYIAEWSQPGALTAMLNWYRASPLVVPPP
                     GAAVPVPDWVLGVFPKVRIPTLVIWGMRDPALLPVQLDGLGRLVEELAVVRLPEAGHF
                     APWEAPDTVAEALGPFLAREPAATAPVR"
     gene            2447..2911
                     /gene="nusB"
                     /locus_tag="VFU20_05390"
     CDS             2447..2911
                     /gene="nusB"
                     /locus_tag="VFU20_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU5285928.1"
                     /translation="MSSSARSRSRSAARLAAVQALYQQEMEGTPVAQLLKEFHDHRLG
                     AMIEDAQYVDAEREFFDDLVTGVGSRCAEIDAAISARLADGWSLARLDRPMRAILRAG
                     AYELLARRDVPVGSVISEYVDVAHAFYDKRESGFVNGLLDAIAKEARAAPAS"
     gene            2911..3873
                     /gene="thiL"
                     /locus_tag="VFU20_05395"
     CDS             2911..3873
                     /gene="thiL"
                     /locus_tag="VFU20_05395"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEU5285929.1"
                     /translation="MDETEAIARVRRFATDPAARGLLDDAALFEGLVLTHDSIAEGVH
                     FLPEDPPASVGWKLVAVNLSDLAAKGAEPAAALLSLTVSGDSDWDEAFLGGIEAACAS
                     YGLPLIGGDTIALPQGAPRVFGLTAIGRAGPRTPARSGAKPGDRLWLAGTVGDSAAGL
                     ALLAKDRKAAGLLVDSYRRPVPQLAVGRALAHHATAMIDVSDGLLLDARRLAEASGCG
                     AAIDLDALPLSEAFVAERGDGLDAQLFAATGGDDYALLAALPAGVDPFTLSLPSGTSI
                     ACIGSLEAGGETLRLASKGEPVALPEVLGHEHRGNPASPVAGRS"
     gene            4045..>5372
                     /locus_tag="VFU20_05400"
     CDS             4045..>5372
                     /locus_tag="VFU20_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010336932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HEU5285930.1"
                     /translation="MNLLLIAIACGVVALLYGILTSRQVLASSAGNQRMIEVAGAIQE
                     GASAYLRRQYTTITGVGVAVAAVVFIFLGGLSALAFLIGAILSGVTGFIGMNISVRAN
                     VRTAEAARTSLQRGLTVAFRAGAVTGLLVAGLALLAISLLFWYLTGPAGHAANDRSVV
                     TALTALALGASLVSIFARLGGGIFTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVG
                     DNVGDCAGMAADLFETYVVTVGATMVLIALLVSTGAEQMLALMSLPLVIGGVCILTSI
                     VGTYFVRLGRKESIMGALYKGFWVTAVLSVGAIWAVMSLTLGDLNAVIGSAREAAGAD
                     VDPAVAAGAVADGFTGWDLFWCSMVGLAVTGLIVWITEYYTGTNYRPVQSIARASQTG
                     HGTNVIQGLAVSLESTALPTLVICVGIILSYQLAGLIGIAFAATAMLAMAG"
BASE COUNT          718 a         1934 c         1866 g          854 t
ORIGIN      
        1 ctccagacag gggtcgctcc ggcggtcggc gagaaggccc ggaccgagcg ctcccagccg
       61 ccgtagggcg tctgggcggc cgaaatctcc tcggggctca tgccgtcgaa ataattgtag
      121 gcggaattgt gctcgcggac atggtcgagg cacaggaagc gccagctgcc cgggccgttg
      181 aaattggccg gctcgagcgg cgccttatac tcgccgggct cgccgcagcc ggggaccgcg
      241 cagcgatccc gcgctccctc gacccagccg tgcattctct ggttacgcga cgccacttga
      301 cttgctcact gctccgccgg cttcctcgcc tgttgaaccg gcctatatag acgccatgcg
      361 cgatgcttcc accggcccgg tcgcaaccga gatgcttcgg cggctcaact cgtcgctatc
      421 cccgagccgg atcgagctca tcgacgacag cgagcagcac cgcggccacg gcggctacca
      481 ccccgccggg gagagccatt tcacgctccg gatcgagagc ccggcgttcg ccggcaagtc
      541 gcggatcgag cgccagcgga tggttcacaa ggccttgggc gacctgctcg acgagcgcgt
      601 ccacgcgctg tcgatccggg cgaccgcacc gggtgaggca tgaccgacaa tccgatcgcc
      661 cagaccatcg ctccggtcac ccacgacctc ggcgcgttca aggtccgccg ggcgcttccg
      721 acccgcgaac ggacgatggt cgggccgttc atcttcgtcg acgagttcgg cccggcgcgg
      781 ctgccgcccg gccaggggat ggacgtccgc ccgcacccgc acatcaacct cgccaccgtc
      841 acctatctgt tcgacggcgc gatcgagcac cgcgattcgc tgggctcgcg ccaggtgatc
      901 gagcccgggg cgatcaacct gatgaccgcc ggccggggca tcgtccattc ggagcgctcg
      961 ccggccgaca gccgcgccgc gggcccgagc ttctacggga tgcagacctg gctcgcgctg
     1021 cccgacggcg gcgaggaggt cgacccggcg ttcgaccatg tgccggccgg cgggctgccg
     1081 ttggtcgagt ccggcggcgc gtcggcgcgg gtgctgatgg gcacgctgtg gggggcgagc
     1141 gcggcgaccc cgtgccattc gccgaccatc tatgccgaca tcgccctgac cgtcggcggc
     1201 agcgtgccga tcgactccga ggccgacgag cgcgcgctga tgctggtcgg cggcgaggcc
     1261 gagctcgacg gagtgccgct cgagccgttc gcgctctatg tgctcgcccc gggccattcg
     1321 gcgcggctgt cgagcgcgag cgaggcgcgg gcgatgctga tgggcggcgg ggcgttttcc
     1381 accccgcgcc acgtctactg gaacttcgtc tcctcgtcgc gcgagcggat caaccaggcc
     1441 aaggacgact ggcaggcgat gcgcttcccg ctgatcccgg gcgacgacca agagttcatc
     1501 ccgctgcccg cggtgccgac caccgtcagc tacccatgag cgagtttcgc cggattgcgc
     1561 tcgcgaccgg cgtcaccttg aatgtcgcgg tggccggcga cccggccgcg ccaccggtgg
     1621 tcctgctcca cggcttcccc gaatcgcacc gcacgtggcg cgggctcgcg ccgctgctcc
     1681 aggaccgctt ccgcctggtc atgcccgacc agcgcgggtt cgcggggtcg gaccgcccgc
     1741 agggcgtcga tgcgtatcgg acggacaagg tcgtcgcgga cctgttcgcg ctggccgacg
     1801 cgctcggggt cgaccgcttt gcactggtcg gccacgactg gggcggagcg gtcgcctggg
     1861 cggcggcgac ccgcggcgac ccgcggctga cccggctggc tatcgtcaac gcgccgcatc
     1921 cggtcgtctt ccagaagagc ctggtcgagg acgccgggca gcgcgccgcc tcgcaatata
     1981 tcaatgcgtt ccgggcgccg ggcttcgaga ggctggtcga ggcgatgggt tacgaagcct
     2041 ttttcgagaa aagcttcggg ccgcacgtcg acctggcgcg gatccccgag gccgagaagc
     2101 gccagtatat cgccgaatgg tcgcagcccg gcgcgttgac ggcgatgctc aactggtacc
     2161 gcgcctcgcc gctggtcgtc ccgccgccgg gcgccgccgt gccggtcccc gactgggtgc
     2221 tcggcgtgtt ccccaaggtt cggatcccga cgctcgtcat ctgggggatg cgcgatccgg
     2281 cgctgctccc ggtccagctc gacgggctcg gccggctggt cgaggagctg gccgtcgtgc
     2341 gcctgcccga ggcaggccat ttcgcgccgt gggaagcgcc ggacaccgtg gccgaggcgc
     2401 ttgggccctt cctcgcgcgc gaacccgccg ctacagcgcc cgtccgatga gcagctccgc
     2461 ccgttcccgc tcgcgctccg ccgcccgcct cgccgcggtg caggcgctgt accagcagga
     2521 gatggagggg accccggtcg cgcaactgct caaggagttc cacgaccacc gcctcggcgc
     2581 gatgatcgag gacgcgcaat atgtcgacgc cgagcgcgag ttcttcgacg atctggtgac
     2641 cggcgtcggg tcgcgctgcg ccgagatcga cgccgccatc tcggcgcggc tggccgacgg
     2701 ctggagcctc gcccggctcg accggccgat gcgcgcgatc ctgcgcgccg gcgcctacga
     2761 gctgctcgcc cgccgcgacg tgcccgtggg ttcggtgatc tccgaatatg tcgacgtcgc
     2821 ccacgccttc tacgacaagc gcgagagcgg cttcgtcaac ggcctgctcg acgcgatcgc
     2881 caaagaagcg cgggccgctc cggcgagctg atggacgaga ccgaggcgat tgcgcgggtc
     2941 cgcaggttcg cgacggaccc ggcggcgcgc gggctgctcg acgacgcggc tctgttcgag
     3001 ggtttggtcc tcacccacga cagtatcgcc gagggcgtgc atttcctgcc cgaagacccg
     3061 ccggcgagcg tcggctggaa gctggtcgcg gtcaatctgt ccgacctcgc cgccaagggc
     3121 gccgaacccg ccgccgccct gctgtcgctg acggtcagcg gcgacagcga ctgggacgag
     3181 gcgttcctcg gcgggatcga ggccgcgtgc gccagctacg ggctgccgct gatcggcggc
     3241 gacaccatcg cgcttccgca aggagcgccg cgggtgttcg ggctgaccgc gatcggccga
     3301 gcggggccgc gcaccccggc gcgcagcggc gcgaagccgg gcgaccggct gtggctggcc
     3361 gggacggtgg gggattcggc ggccgggctg gccttgctgg caaaggaccg caaggccgcg
     3421 gggctgctgg tcgacagcta ccggcggccg gtaccgcagc tcgccgtggg ccgggcgctg
     3481 gcgcaccatg cgaccgcgat gatcgacgtg tcagacgggc tgctgctcga cgcccggcgg
     3541 ctggccgagg cgagcggctg cggagcggcg atcgacctgg acgcgctgcc gctgtccgag
     3601 gcctttgtcg ccgagcgcgg cgacgggctc gacgcgcagc tgttcgcggc gaccggcggc
     3661 gacgattacg cgctgctcgc ggcgctgccc gccggggtcg atccctttac cctttcttta
     3721 ccttccggga ccagcatcgc gtgcatcggg tctctcgaag ccggcggcga aacgcttcgc
     3781 ctggccagca agggagagcc ggtcgcgctg ccggaggtcc tgggtcatga acatcgcggc
     3841 aatcccgctt cgccagtggc tggtcgctct tagcggcctg gcttcgggca tggccgtcgc
     3901 gctcctgatc aactgaccgg ccgactcctt ccttcatccc tcgggcaggt tgcaacccgc
     3961 catttcccca cataaagggg cgcgaatccg ccggctgcgt cagcatcgcc ggcttttcca
     4021 atttgccgcc agacggggat aagaatgaac ctgcttttga tcgcgatcgc ctgcggcgtc
     4081 gtcgctttgc tctatggcat cctcaccagc cgccaggtgc tcgcctcgtc ggccggcaac
     4141 cagcgcatga tcgaggtcgc gggggcgatc caggaaggcg catcggccta ccttcgccgc
     4201 cagtacacga ccatcaccgg ggtcggagtc gcggtcgccg cggtggtgtt catcttcctc
     4261 ggcgggttga gcgcgcttgc cttcctgatc ggcgcgatcc tgtccggcgt caccggcttc
     4321 atcggcatga acatttcggt ccgcgccaac gtccgcaccg cggaggccgc gcgcaccagc
     4381 ctgcagaggg gcctgacggt cgccttccgc gccggcgcgg tcaccgggct gctggtcgcc
     4441 gggctcgcgc tgctcgcgat ctcgctgctg ttctggtacc tgaccggccc cgccggccac
     4501 gccgccaacg accgctcggt ggtcaccgcg ctgaccgctt tggcattggg cgccagcctg
     4561 gtctcgatct tcgcgcgtct cggcggcggc atcttcacca aggccgctga cgtcggcgcc
     4621 gacctggtcg gcaaggtcga ggccgggatt cccgaagacg acccgcgcaa cccggccgtg
     4681 atcgccgata acgtcggcga caatgtcggc gactgcgccg ggatggccgc cgacctgttc
     4741 gagacctatg tcgtcaccgt cggcgccacc atggtgctga tcgcgctgct cgtcagcacc
     4801 ggcgccgagc agatgctggc tctgatgagc ctgccgctgg tcatcggcgg agtgtgcatc
     4861 ctgacctcga tcgtcggcac ctatttcgtg cgcctgggcc gcaaggaatc gatcatgggc
     4921 gcgctctaca agggcttctg ggtcaccgcc gtcctgtcgg tcggcgcgat ctgggccgtc
     4981 atgtcgctga ctctcggcga cctcaacgcg gtcatcggca gcgcccgcga ggccgccggg
     5041 gccgacgtcg acccggcggt cgccgccggc gcggtcgccg acggcttcac cggctgggac
     5101 ctgttctggt gctcgatggt cggcctcgcg gtgaccggcc tcatcgtctg gatcaccgaa
     5161 tattacaccg gcaccaacta tcggccggtc cagtcgatcg cccgggcatc gcagaccggg
     5221 cacggcacca acgtcatcca ggggctcgcg gtcagcctcg aatcgaccgc cttgccgacc
     5281 ctggtgatct gcgtcgggat catcctttcc taccagctgg cgggcctgat cggaatcgcc
     5341 ttcgccgcca cggcgatgct ggcgatggcc gg
//
LOCUS       DASTZO010000195         2622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_172702,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000195 DASTZO010000000
VERSION     DASTZO010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2622
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_172702"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="VFU20_05405"
     CDS             <1..506
                     /locus_tag="VFU20_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5285931.1"
                     /translation="GQGTVGLEILDQLARAGEPPPARIVAPAGGGGLASGIALACPDA
                     EIVCVEPERWDDLRRSLELGEIVSVPADAPPTLCDALQTPRVSPITFGILRQRGASAV
                     AVSDEEVVAAVRFAWSEHRLVVEPGGAAALAALLAGKAEAVGGTVAVVSGGNVDPALH
                     ARIVSGD"
     gene            complement(503..754)
                     /locus_tag="VFU20_05410"
     CDS             complement(503..754)
                     /locus_tag="VFU20_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285932.1"
                     /translation="MATADQQARLEEAFERIEETILPCIAMMLETLLDVSAGVGETNP
                     KALAAELRTLAAELEALTRAVERSSPVHVEPGEYKTASA"
     gene            806..973
                     /locus_tag="VFU20_05415"
     CDS             806..973
                     /locus_tag="VFU20_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285933.1"
                     /translation="MNDFASAAMAVAMIAAFLLAAGGVRLALQRENRSRGLLMIAAAA
                     VLVGNVLVWTL"
     gene            complement(970..1623)
                     /locus_tag="VFU20_05420"
     CDS             complement(970..1623)
                     /locus_tag="VFU20_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014848356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5285934.1"
                     /translation="MPEVIFPGPEGRLEGRFHPGTRPRAPVALILHSHPQAGGTMNNK
                     MVQLLYQTFVRRGFATLRFNFRGVGKSQGTFDNGIGELSDAASALDWVQQIHPEAETT
                     WVAGVSFGAWIGMQLLMRRPEIKGFISIAPPANMYDFSFLAPCPSSGIIIQGEADEVV
                     TPGAVQKLVDKLRTQRHITIHHDVIPGANHFFANELDLLMKSVDNYLDMRLAKDLKR"
     gene            1741..>2622
                     /locus_tag="VFU20_05425"
     CDS             1741..>2622
                     /locus_tag="VFU20_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5285935.1"
                     /translation="MARTRLYLDHAATTPVLAEAQSAMAEALGHWANPSSPHADGRAA
                     RAALEEARAGIAEALGWRHDVILTSGASEAIAIAGSRAKAGRRQIGATEHDAVVAAMG
                     PDAAVVPVDEQGRIDLDALDRALADDPALVAIQQVNNETGVIQPLDEIAARVRAAGSL
                     LLADCAQGAGKVRLPDADFIAVSAHKLGGPPGMGALLVRDIATLAPSGGQEKGYRRGT
                     ENLPAAVGFAAALRSRAFTEAMPRLADLRSKLEQAIASAGGTIVAAGSKRTPAIGAYT
                     MPGVSSAAQLVQFDLAGI"
BASE COUNT          359 a          878 c          986 g          399 t
ORIGIN      
        1 ccggccaggg caccgtcggg ctcgagatcc tcgaccagct ggcgcgggcc ggcgagccgc
       61 cgcccgcgcg catcgtcgcc ccggccggcg gcggcgggct ggcgtcgggc atcgccctgg
      121 cgtgccccga tgcggagatc gtctgcgtcg agcccgagcg ctgggacgac ctgcgccgct
      181 cgctcgagct cggcgaaatc gtgtcggttc cggccgacgc gccgccgacg ctgtgcgacg
      241 cgctccagac cccgcgcgtg tcgccgatca ccttcggcat cctccgccag cgcggggcga
      301 gcgcggtcgc ggtcagcgac gaggaggtcg tcgcggcggt ccgcttcgcc tggagcgagc
      361 accggctggt cgtcgagccc ggcggcgcgg ccgcgctcgc cgcgcttctg gcggggaagg
      421 ccgaggccgt gggcgggacg gtggcggtgg tgtcgggcgg gaacgtcgat ccggcgctgc
      481 acgcgcggat tgtgagcggc gattaagccg atgcggtctt atactcgccc ggctcgacgt
      541 ggaccgggct cgagcgctcg accgcgcggg tcagcgcctc gagctcggcg gccagcgtgc
      601 ggagctcggc ggccagcgcc ttgggattgg tctcgccgac cccggcggag acatcgagca
      661 gggtttcgag catcatcgcg atgcacggca ggatcgtctc ctcgatccgc tcgaacgcct
      721 cctcgaggcg cgcctgctgg tcggcagtgg ccatgccccg cgttttggcc cagcatagtt
      781 aaaccttgat gaagggtgtt ggggtttgaa cgatttcgcc tccgcagcga tggccgtcgc
      841 gatgatcgcc gcgttcctgc tcgccgcggg cggggtgagg ctggcgctgc aacgcgaaaa
      901 ccgctcgcgc ggcctgctga tgatcgccgc ggccgcggtg ctggtcggca acgtgctggt
      961 gtggacgctt tagcgcttca gatccttggc caggcgcatg tcgagataat tgtcgacgct
     1021 cttcattagc aggtcgagct cgttggcgaa gaagtggttg gcgcccggga tcacgtcgtg
     1081 gtggatggtg atgtggcgct gggtgcggag cttgtcgacc agcttctgga ccgcgcccgg
     1141 ggtgacgacc tcgtcggcct cgccctggat gatgatcccc gaggacgggc acggcgcgag
     1201 gaagctgaaa tcgtacatgt tggcgggcgg ggcgatcgag atgaagccct tgatctccgg
     1261 ccggcgcatc aggagctgca tcccgatcca cgcgccgaag ctgaccccgg ccacccaagt
     1321 ggtctcggcc tcggggtgga tctgctgcac ccagtcgagc gccgaggccg cgtcgctcaa
     1381 ctcgccgatg ccgttgtcga acgtgccctg gctcttgccg actccgcgga agttgaagcg
     1441 cagcgtcgcg aagccccgcc ggacgaacgt ctggtagagc agttgcacca tcttgttgtt
     1501 catcgtcccg ccggcctgcg ggtgcgagtg caggatcaga gcgaccgggg cgcggggccg
     1561 ggtgcctgga tggaaacggc cttccaggcg gccttccggg ccagggaaaa tcacttcggg
     1621 catggatttg gcgtgctcgc gtaaaaaagt cggtgcttcg gccgacgggt gcgccgcggc
     1681 ggcggctcct atataggctc ggcgcttgaa ctggcaatcg gcaggaatcc aggcattctg
     1741 atggctcgga cgcgtctcta cctcgaccat gcggcgacca ccccggtgct cgccgaagcg
     1801 cagtcggcga tggctgaggc gctcgggcat tgggccaacc cgagctcgcc gcacgccgac
     1861 gggcgggcgg ccagggccgc gctcgaggaa gcgagagcgg ggatcgccga agcgctcggc
     1921 tggcgtcatg acgtcatcct gacgtccggg gcgagcgagg cgatcgcgat cgccgggtcg
     1981 cgggcgaagg ccggccggcg gcagatcgga gcgacggagc acgatgcggt ggtcgcggcg
     2041 atgggccccg acgcagcggt cgtcccggtc gacgagcagg ggcggatcga cctcgacgcg
     2101 ctcgaccggg cgctcgccga cgatccggcc ctggtggcga tccagcaggt caacaacgag
     2161 accggcgtca tccagccgct cgacgagatc gcggcgcggg tccgggcggc cggctcgctg
     2221 ctgctcgccg attgcgcgca gggggcgggc aaggtgcggc ttcccgacgc ggacttcatc
     2281 gcggtttcgg cgcacaagct cggcggcccg ccggggatgg gcgcgctgct ggtccgggac
     2341 atcgccacgc tcgccccgag cggcgggcag gaaaagggct accgccgcgg caccgagaac
     2401 ctgcccgccg cggtcggctt cgccgcggcg ctgcgctcgc gggccttcac cgaggcgatg
     2461 ccgcggctgg cggaccttcg cagcaagctc gagcaagcca ttgcttcggc tggcggaaca
     2521 atcgtcgccg ccggcagcaa gcggaccccg gccatcggcg cctacacgat gccgggcgtc
     2581 tcgagcgccg cccagctggt ccagttcgac ctcgccggga tc
//
LOCUS       DASTZO010000196         2241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1035011,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000196 DASTZO010000000
VERSION     DASTZO010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035011"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VFU20_05430"
     CDS             complement(<1..1242)
                     /locus_tag="VFU20_05430"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123853.1"
                     /note="catalyzes the uridylylation or deuridylylation of
                     the PII nitrogen regulatory protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein"
                     /protein_id="HEU5285936.1"
                     /translation="MIALEPVDNRRAIVDRRALAERIAAVRTGKRRADQVTAILREAL
                     ASGREEIARRLSEEPGRGRAAARATAFLHDQIVRLAYDSATPGTQGVALVGLGGTGRG
                     EMAPYSDLDLMFLTAAKPTRADGEAAEAILHILWDLKLKVGHSLRSVSELVALAKKDM
                     TVRSALLEARLLWGEEQLFDLAMRRFRKEVVAGTAAEFVAAKLAERDARHVRMGDSRY
                     VVEPNVKDGKGGLRDLHTLYWIGKYVHDVERPSDLVGAGLLTAAEFQSFERAERFLWA
                     VRCHLHLAAGRAEERLGFDQQKHIAQVMHYADRPGKSAVERFMQFYFLNAKTVGDLTG
                     LFLAQLDEQLGKKGFRFALPTLRRRPKRLNGFTLDRGRLSIPSDTFLREQPVRLLELF
                     ALAARERLEIHPNAMRAATRDA"
     gene            complement(1239..>2241)
                     /locus_tag="VFU20_05435"
     CDS             complement(1239..>2241)
                     /locus_tag="VFU20_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU5285937.1"
                     /translation="LALAAAPTITATAEAIARIDVAASHAARAAEGGWTRPQLTDEPC
                     LEIEAGRHPVVESALGAAGERFVANDLAMSASDRLWLITGPNMGGKSTFLRQAALIVV
                     LAQAGGFVPAARARIGLVDRLFSRVGASDNLARGRSTFMVEMVETAAILAQATPRSLV
                     ILDEIGRGTSTYDGLAIAWAVVEAVHDQLQCRTLFATHYHELTRLAGRLDCLSLHHVR
                     AREWKGDLVLLHEVADGAADRSYGIAVAKLAGLPPPVVARARSVLAKLEAGRDATGGI
                     AAGLDDLPLFAAAHEPEPVPDALADALAGIEPDSLSPREALEQLYRLKRLAAERGSA"
BASE COUNT          325 a          789 c          812 g          315 t
ORIGIN      
        1 ggcgtcgcgg gtcgcggcgc gcatcgcatt gggatggatc tcgagccgct cgcgggcggc
       61 gagcgcgaac aattcgagca ggcgcaccgg ctgctcgcgc aggaatgtgt ccgacgggat
      121 cgacagccgg ccgcggtcga gggtgaagcc gttcaaccgc ttgggccgcc ggcgcagggt
      181 cggcagcgcg aagcggaacc ccttcttgcc caattgctcg tccaattgcg cgagaaacag
      241 cccggtcaag tcgccgaccg tcttggcgtt cagaaaatag aattgcatga agcgctcgac
      301 cgccgatttc cccggccggt cggcgtagtg catcacctgg gcgatatgct tctgctggtc
      361 gaagccgagc cgttcctcgg cgcggccggc ggcgaggtgc aggtggcagc gcaccgccca
      421 caggaagcgc tcggcgcgct cgaagctctg gaactcggct gcggtcagca gcccggcgcc
      481 gaccaagtcg gacggccgct cgacgtcgtg gacatatttg ccgatccagt agagcgtgtg
      541 gaggtcgcgc agccctccct tgccgtcctt gacgttgggc tcgacgacgt agcggctgtc
      601 gcccatccgg acgtggcggg cgtcgcgctc ggcaagcttg gcggcgacga actcggccgc
      661 ggtgccggcg acgacttcct tgcggaagcg gcgcatggcg aggtcgaaca gctgctcctc
      721 gccccacagc aagcgcgcct cgagcaaagc gctgcgcacc gtcatgtcct tcttggcgag
      781 cgcgaccagc tcggacaccg agcgcagcga atggccgacc ttcaatttca gatcccacaa
      841 gatgtggagg atcgcctcgg ccgcctcgcc gtcggcgcgg gtcggcttgg cggcggtcag
      901 aaacatcagg tcgaggtcgc tgtacggcgc catttcgccg cgcccggtgc cgccgaggcc
      961 gaccagtgcg acgccctgcg tcccgggcgt cgcggaatcg taggcgaggc ggacgatctg
     1021 gtcgtggagg aaggcggtgg cgcgcgccgc ggcgcggcct cgaccgggct cttcggacag
     1081 ccgccgcgca atctcctcgc gtccggaagc gagtgcctcg cgaaggatcg cggtgacctg
     1141 gtcggccctg cgcttgcccg tacggacagc cgcgatccgc tcggcgagcg cgcgccggtc
     1201 gacgatcgcc cggcgattgt cgacgggctc gagcgcgatc atgccgagcc ccgctcggcg
     1261 gcgaggcgct tcaatcggta gagctgctcg agcgcctcgc gcggggacag cgaatcgggc
     1321 tcgattcccg ccagcgcgtc ggcgagcgcg tccggcaccg gctcgggctc gtgcgcggcg
     1381 gcgaacagcg gcaaatcgtc gaggcccgcg gcgatcccgc cggtcgcgtc gcgcccggcc
     1441 tcgagcttgg cgagcaccga cctggcccga gcgaccaccg gcggcggcag cccggcaagc
     1501 ttggcgacgg cgatcccgta gctgcggtcg gcggcgccgt cggcgacttc gtggagaagc
     1561 accagatcgc ccttccattc gcgcgcccgg acatggtgca gcgacaggca gtcgagccgc
     1621 ccggcaagcc gcgtcagctc gtgataatgg gtcgcgaaca gggtccggca ctgcagctgg
     1681 tcgtgcaccg cctcgaccac cgcccaggcg atcgccagcc cgtcgtaggt cgaggtgccg
     1741 cggccgatct cgtcgaggat caccaggctg cgcggcgttg cctgcgccag gatcgccgcg
     1801 gtctcgacca tttcgaccat gaaggtcgag cggccgcggg cgagattgtc cgacgccccg
     1861 acccggctga acagccggtc gacgaggccg atccgggcgc gcgcggcggg gacgaaaccc
     1921 ccggcctggg cgagcacgac gatcaaggcc gcctggcgga ggaaggtcga cttgccgccc
     1981 atgttgggcc cggtgatcag ccacagccgg tcgctcgcgc tcatcgccag gtcgttggcg
     2041 acgaaccgct cgcccgcggc gccaagcgcg ctttcgacca ccgggtgccg gcccgcctcg
     2101 atctccaggc acggctcgtc ggtcagctgc gggcgggtcc agccaccctc ggccgcccgc
     2161 gccgcgtggg atgcggcgac gtcgatccgg gcgatcgcct cggcggtcgc ggtgatcgtc
     2221 ggcgcggccg ccaaagcgag c
//
LOCUS       DASTZO010000197         2929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_434390,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000197 DASTZO010000000
VERSION     DASTZO010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_434390"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="VFU20_05440"
     CDS             complement(<1..432)
                     /locus_tag="VFU20_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEU5285938.1"
                     /translation="MAGKLWKAIDSHGARRLDELFKAEPDRLSRLAFEVAGIYFDWSK
                     THLDAGLVEAFVALSEKRGFAAAREALFSGGIVNPSEGQPAEHVAERGSGAPDAVDLA
                     TARRQRMRALIDAIEGGAFGDVTALLHIGIGGSVLGPALLVD"
     gene            516..1358
                     /gene="lepB"
                     /locus_tag="VFU20_05445"
     CDS             516..1358
                     /gene="lepB"
                     /locus_tag="VFU20_05445"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5285939.1"
                     /translation="MRSPRSTSRTQGAQGRDGGLIRFLLLLALAAWALRSFVVAPFSI
                     PTGSMLPTLYVGDYLFVAKWPYGYSRYSFPFGFPPFDGRILSNLPRRGDVAVFRPPGA
                     KIDFIKRVIGLPGDTVEVKGGMLVLNGRPLPRRSMRPFAMPISPNSPCKVVPPASPFV
                     ASLGQGRSYCLYPAYRETLPGGPGYTVLDQVENPRADDFPAIKVPQGHVFLMGDNRDD
                     SLDSRFALAEGGIGLVPVENLVGRALVTFWSTDGSASYVKPWTWFTALRGGRIGNGYT
                     GEPE"
     gene            1355..2017
                     /gene="rnc"
                     /locus_tag="VFU20_05450"
     CDS             1355..2017
                     /gene="rnc"
                     /locus_tag="VFU20_05450"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEU5285940.1"
                     /translation="MSDLAAFVRDTLGHEPGDVALFERALTHSSLNRDSYERLEFLGD
                     RVLGLIVGRWLYERFGAEPEGQLSRRYNALVAREVCAEIGRELGVPALIRLGKQARGD
                     GASDSDNVVGDVVEALIGALMLDGGIDAAEAFVRRAWGPRVDRQGRAPVHSKSALQEL
                     AAARGLATPAYQVVARTGAQHAPRFTVRVAIRNAGEAEGHGSSKQEAETAAATALLEK
                     LA"
     gene            2014..2907
                     /gene="era"
                     /locus_tag="VFU20_05455"
     CDS             2014..2907
                     /gene="era"
                     /locus_tag="VFU20_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU5285941.1"
                     /translation="MSSRCGFIAVIGAPNAGKSTLVNALVGQKVAIVSAKAQTTRARL
                     MGIAIEGDAQILLVDTPGIFAPRRRLDRAMVAAAWAGAEDADLVLLVIDAAARLGERV
                     EAMLAGVEGSHRPLMIVLNKVDIARKEDLLTLAAQLSERLGPKALFMVSATTGDGVPD
                     LKRALAEAMPEAPWHFPADQLSDATERMVAAELTREQIYEQLHAELPYSSVVATEKWE
                     DRKDGSTVIHQQILVDRDSQKAIVLGRGGARLKSIGQAAREAIAEHLGRKVHLFLHVK
                     VNPRWDEDRSLYRDIGLEWAE"
BASE COUNT          436 a         1072 c          993 g          428 t
ORIGIN      
        1 atcgaccagc agcgccggcc cgagcaccga gccgccgatg ccgatgtgca ggagcgcggt
       61 gacgtcgccg aacgcaccgc cctcgatcgc gtcgatcagc gcccgcatcc gctggcggcg
      121 ggcagtggcg aggtcgaccg cgtcgggcgc tccgctgccg cgctcggcga catgttcggc
      181 cggctggccc tcgctcggat tgacgattcc gccggagaac agcgcctcgc gcgccgccgc
      241 gaacccgcgc ttttcgctca gcgcgacgaa cgcctcgacc agccccgcgt cgaggtgcgt
      301 cttcgaccag tcgaaataga tgccggcgac ctcgaaggcg agccggctca ggcggtcggg
      361 ctcggccttg aacagctcgt cgaggcgccg ggcgccatga gagtcgatcg ccttccagag
      421 cttgcccgcc atgttaagcg cctcatagtc ggcggccctc caaatgccac ccccgccttg
      481 acgggctcgc gccggcccga aagaagcacc ggctgatgcg ctccccccgc tccacatcgc
      541 gaacccaagg cgcccagggc cgcgacggcg gcctgatccg cttcctgctg ctgctcgcgc
      601 tcgccgcctg ggcgcttcgg agcttcgtcg tcgctccgtt cagcatcccg accggatcga
      661 tgctcccgac cctctacgtc ggcgattatc tgttcgtcgc caaatggccg tacggctatt
      721 cgcgctacag cttcccgttc ggcttcccgc cgttcgacgg gcggatcctc tccaacctgc
      781 cccggcgcgg cgacgtcgcc gtgttccggc cgcccggcgc gaagatcgac ttcatcaagc
      841 gcgtgatcgg gcttcccggc gacacggtcg aggtcaaggg cgggatgctg gtcctcaacg
      901 gccggccgct gccgcgccgg tcgatgcggc cattcgcgat gccgatcagc cccaacagcc
      961 cgtgcaaggt ggtgccgccg gcgagcccgt tcgtcgccag cctcggccag ggccgcagct
     1021 actgcctcta tcccgcctat cgcgagacgc ttccgggcgg gccgggctac accgtgctcg
     1081 accaggtcga gaacccgcgc gccgacgact tcccggcgat caaggtgccg cagggccatg
     1141 tgttcctgat gggcgacaac cgcgacgaca gcctcgacag ccgctttgca ctggccgagg
     1201 gcggcatcgg cctggtcccg gtcgagaacc tcgtcggccg cgccctcgtg accttctggt
     1261 cgaccgacgg cagcgcgtcc tacgtcaagc cgtggacctg gttcacggcg ctgcgcggcg
     1321 gccgcatcgg caacggctac accggggagc ccgaatgagc gacctcgccg cgttcgtccg
     1381 cgacacgctc ggccacgagc ccggggatgt cgccttgttc gagcgagcgc tgacccattc
     1441 gagcctcaac cgcgacagct atgagcgcct ggagttcctc ggcgaccggg tgctcggcct
     1501 gatcgtcggc cgctggctct acgagcgctt cggcgccgag cctgaaggcc aattgtcgcg
     1561 gcgctacaat gccctggtcg cgcgcgaagt ctgcgccgag atcggccgcg aactcggcgt
     1621 cccggccctg atccgcctgg gcaagcaggc ccgcggcgac ggcgccagcg acagcgacaa
     1681 tgtcgtcggc gacgtcgtcg aggccttgat cggggcgctg atgctcgacg gcgggatcga
     1741 cgccgccgag gcgttcgtgc gccgcgcctg gggcccgcgc gtcgaccgcc agggccgggc
     1801 accggtccat tcgaaatccg cgctccagga gctcgccgcc gcgcgcggcc tcgcgacccc
     1861 ggcctaccag gtcgtcgccc ggaccggcgc ccagcacgcg ccgcgcttca ccgtccgggt
     1921 cgcgatccgc aacgccggcg aggccgaggg ccacggctcg agcaagcagg aagccgaaac
     1981 cgccgccgcg acggcgcttc tggagaagct cgcatgagca gccgctgcgg gttcatcgcg
     2041 gtgatcggcg ctcccaacgc cggcaagtcg acgctggtca atgcgctggt cggccagaag
     2101 gtcgcgatcg tcagcgccaa ggcgcagacc acccgcgcgc ggctgatggg catcgcgatc
     2161 gagggcgatg cgcagatcct gctggtcgac acgccgggca tcttcgctcc gcgccgccgg
     2221 ctcgaccggg cgatggtcgc cgctgcctgg gccggcgccg aggacgccga cctggtgctg
     2281 ctggtgatcg acgccgcggc caggctcggc gagcgggtcg aggcgatgct cgccggagtc
     2341 gagggcagtc accggccgct gatgatcgtc ctcaacaagg tcgacatcgc ccgcaaggaa
     2401 gacttgctca ccctcgccgc gcagctgtcg gagcgcctgg gccccaaagc cctgttcatg
     2461 gtctcggcga ccacgggcga cggcgttccc gacctcaagc gcgcgctcgc cgaggcaatg
     2521 cccgaagcgc cgtggcactt ccccgccgac cagctcagcg acgccaccga gcggatggtc
     2581 gccgccgagc tgacccgcga gcagatctac gagcagctgc acgccgagct gccttattcg
     2641 tcggtggtcg cgaccgagaa atgggaggac cgcaaggacg gctcgaccgt gatccaccaa
     2701 cagatcctgg tcgaccgcga cagccagaag gcgattgtgc ttgggcgtgg cggtgcgcgg
     2761 ttgaagagca tcggccaggc ggcgcgcgaa gcgatcgccg agcatctcgg ccgcaaggtg
     2821 catttgttcc tgcacgtgaa ggtgaatccc cgctgggacg aggaccgctc gctttaccgc
     2881 gacatcgggc tggagtgggc ggagtgatac ctaatcgagt cgactatgt
//
LOCUS       DASTZO010000198        10345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1139349,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000198 DASTZO010000000
VERSION     DASTZO010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 10345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10345
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1139349"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..251)
                     /locus_tag="VFU20_05460"
     CDS             complement(<1..251)
                     /locus_tag="VFU20_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HEU5285942.1"
                     /translation="MTRGLAPVDRRTLLIGGGAGIGLVVAILAWPRRPSGDLIERKGE
                     RAFGPFLKVGRDGRVTVAVPQVETGQGAWTALPQIVADEL"
     gene            complement(248..1918)
                     /locus_tag="VFU20_05465"
     CDS             complement(248..1918)
                     /locus_tag="VFU20_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HEU5285943.1"
                     /translation="MPSDLDRRFDDMLARVTGPGGRLVIERDSQGRAIVGNFPATVPG
                     LLRTFCALYGDEREALVAGDERLTFADLDRVSESLALALAGRGISKGDRVGIAMRNCP
                     SWIVAYMAILKAGGVATLLNGWWQSHELAHALALAEPGLVIADEPRSKRIESVGGNHD
                     VVTLAVEQPLETALATLLAGSGAAAALPEIAPEDDATLLFTSGSTGEAKGALSTHRQV
                     TTGTYTYSSGLMTLLGLLIEDGREPAGPPRTLLSVPLFHVTGEVPVMLNSFVVGRCMV
                     VMPKWDATEALRLIEKEKITYFVGVPTMSLELLNHPDRDRYDLSSLKDITAGGAPRPV
                     SHVERLKDAFPDAQPALGYGLTETNAVGCINFWGSYAAKPASTGRPARPFVEVAILGA
                     NDTHLPPGQRGEIAIRSAANIKGYWRNPAATQALFTADGYVRTGDIGYFDEDDYLFIV
                     DRKKDIIIRGGENISCQEVEAAIYEHAEVNECAVFGVPDERLGEVPVAIIHPADGSDL
                     DEAGLRAFLESRLAAFKVPARMIFSKEPLPRLGTGKIDRVALKAQYAR"
     gene            1991..2872
                     /gene="lgt"
                     /locus_tag="VFU20_05470"
     CDS             1991..2872
                     /gene="lgt"
                     /locus_tag="VFU20_05470"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009820541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEU5285944.1"
                     /translation="MQPVNAEPDAIAWTDLHLSPVALDLGLFELRWYSLAYLAGIFIG
                     YWYLLRLLKEPGAPMARRHADDLVFYAALGIILGGRLGYVLFYNLGYYLQNPVDILKL
                     WDGGMSFHGGVIGTTVGILYLARKEKLPWLRIHDYVACCVPFGLFFGRLANFVNHELW
                     GAEASVPWAVRFPEVVNGVTILGPPRHPTQLYEALLEGVVLFAILWWMFRKTEARYQP
                     GKLVGAFLFFYGLFRFAVEFVREPDSHLVEFAQASGLHMGQWLSLPMIAGGLYLMMTA
                     ARRRVRVEPTLGPASVA"
     gene            2899..3840
                     /locus_tag="VFU20_05475"
     CDS             2899..3840
                     /locus_tag="VFU20_05475"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU5285945.1"
                     /translation="MIRAGGPISVERYMGLANAHYYATRDPLGVAGDFTTAPEVSQMF
                     GELVGAALADCWRRAGAPADAAYAELGPGRGTLAADALRVLGKAGFAGEVHLVETSPP
                     LRAEQAKRVPGARWHDSVEELPPRPVLLVANEFFDALPVRQWVGGEERRVAVAGDALV
                     FTVDGPIREDSPAREEAVRAVARHLAEHGGVALVIDYGHARSGQGDTLQAVRGHAFAD
                     VLADPGEQDLTAHVDFEALGRTASAAKVTRVVSQGEWLERLGIAARAESLAEVHPERA
                     DEFAAARKRLCDPDEMGALFKVMAIHSRSWPSPAVFE"
     gene            3837..4346
                     /locus_tag="VFU20_05480"
     CDS             3837..4346
                     /locus_tag="VFU20_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5285946.1"
                     /translation="MSIAHRMASEADLPAIDRVFRTGFCDTFGHLYRSEDLDAFLSQF
                     SPQAWLAEHRDPSYAFRIAEARGDAVAFAKLGSLSVPVEPAGPALELRQLYVLRPWQG
                     TGIAQQLMEWALAEARARGIAELYLTVYTDNHRARAFYRRYGFGDVGPYAFMVGSHAD
                     EDIIMRLAL"
     gene            4355..5119
                     /gene="pgeF"
                     /locus_tag="VFU20_05485"
     CDS             4355..5119
                     /gene="pgeF"
                     /locus_tag="VFU20_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HEU5285947.1"
                     /translation="MSVEVLRAANLAGAPHGFLGRRGGVSTGELEGLNVGYGSGDDRD
                     AIAQNRARAIAAVLPGAGLATVHQVHSPTAVVAERPWPHDERPRADGLATDRPGLLLG
                     ILTADCAPVLLADPAAGVVGAAHAGWRGALAGVTDETIAAMERLGARRERIVAAVGPC
                     IGRDSYQVDETFRGRFLGLDPANDRFFSGGPGNRPHFDLAAYVAARLEAAGIAGVEAL
                     ALDTYAEPERFFSYRRSTHRKEADYGRQIAMIGLAP"
     gene            5116..6549
                     /locus_tag="VFU20_05490"
     CDS             5116..6549
                     /locus_tag="VFU20_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HEU5285948.1"
                     /translation="MSAGNSTARWGLWGGLLAFVVLLALPAPAEMPLPAWRTTALVVL
                     MATWWMTQALPLTATALLPFVTFPLFGIMDANAAAASYYSPILFLVLGGAIIALAIER
                     TQLHRRLALAIVQRGGSTPAAMLLAFMTATAIISMIVSNTATTLIMMPIALAVLAAAQ
                     VKPGHSDGFAGALAMGIAFAASIGGLGTLVGSPTNAIAAGIIERATGLHIGFLTWAMY
                     GVPLVLAAIPAAWWILMKVQRVRPTDFDPAQALAGIGTAGQWSAAEKRLVPLIAAVVA
                     GWVAIPFVTPFLPKDSLTDGTIAILGALLLFVIPDGSAEGGKRRAILTWKEANRAPWD
                     VIMLFGGGLALAAGMGQSGLGDWLGVALQPLREVHPLVVAVVLVAIVIVITEFASNVA
                     AASGIIPVVAGIIAATGIDPILLALPAAWAASWGFMLPSGTGPNAIAWATGHIALPRM
                     LKAGLLIDLAGVPLMVGIVWLIAAAMG"
     gene            6559..7884
                     /locus_tag="VFU20_05495"
     CDS             6559..7884
                     /locus_tag="VFU20_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HEU5285949.1"
                     /translation="MAHEEPDTDESELSGATPRRKPKPSATRIGDHKLSASTLMMGYG
                     FDPALSEGSLKPPVFLTSTFVFENAAAGKRFFEGVTGKRPGGAEGLIYSRFNGPNQEI
                     LEDRLSIWEEADDALSFSSGMAAIATLLLTFVQPGDVVVHSGPLYAATETLIAKILGR
                     FGVSWLDFPAGATREEIDSVIAEAKAKGRVSLIYLESPANPTNALVDVEAVAAARNAA
                     FGPNERPPIAIDNTFLGPLWAHPLKQGADLTIYSLTKYAGGHSDLVAGGLVGRQALID
                     PVRMMRNTIGTICDPNTAWMLLRSLETLELRMERAGDNAAKVCAFLRDHPKVEQVGYL
                     GFLEPGSRQADIYARHCTGAGSTFSLYLKGGEKEAFAFLDALTIAHLAVSLGGTETLA
                     SAPAAMTHLSVPDDRKQALAITDNLVRISIGVEDADDLIADFENALKAV"
     gene            complement(7924..8985)
                     /gene="nirK"
                     /locus_tag="VFU20_05500"
     CDS             complement(7924..8985)
                     /gene="nirK"
                     /locus_tag="VFU20_05500"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HEU5285950.1"
                     /translation="MKPAGNRLRIAAAALGALLASAPALAADNEVAAAPAAAPAADIV
                     RDPADLPGPLAVRGPQLVKVHLDTVEVTGRLDDGSTYRYWTYNGKVPGPFVRVRVGDT
                     VEVTMKNAGDSVMAHNVDFHAVTGPGGGGIATMAAPGETKGFTFKALNPGLYVYHCAT
                     PIVAQHIANGMYGLILVEPQEGLSKVDREFYVMQGEIYTEQPNGTKGELTESIDKLLA
                     ETPEYLVFNGAADGLSRKPLKAKVGESVRVFFGVGGPNKTSSFHVIGEIFDKAYPLAS
                     VTSAPMSDVQTITVPPGGAAVTDLRVEVPGRFILVDHALSRLEKGLKGLLEVSGEQRA
                     EIFRAHEEADTAAAQAAGH"
     gene            complement(9037..9492)
                     /locus_tag="VFU20_05505"
     CDS             complement(9037..9492)
                     /locus_tag="VFU20_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017667541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HEU5285951.1"
                     /translation="MRIPFLFLGAAVALAATSPAAAKDHVVKMLNKGQSGPMVFEPAL
                     IKVAPGDTVTFVATDKTHNAESIAGMLPAGAAPFKGKMNQNVSVKFTKPGVYGYKCLP
                     HYGVGMVGVVVVGNASSNLEAAKKVSHPGKAKQAFATLFASSGFRIAAR"
     gene            complement(9648..10319)
                     /locus_tag="VFU20_05510"
     CDS             complement(9648..10319)
                     /locus_tag="VFU20_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family hydrolase"
                     /protein_id="HEU5285952.1"
                     /translation="MADLAVYDLDRTITRRPTYTLFLTHCAMRRQPWRLLFVPFVLLA
                     MLAYAMGLFDRGRLKEICQALLVGRRVRAAELKPLADSFAEATVQHNIRPGARRAIER
                     DREEGRRLVLATASYRLYADSIAERLGFDDVIATGSVIGLDERVHARIAGKNCYGAEK
                     MRMIAEWVEKSGLTGRHGHVRFYSDHASDAPTFEWVDEPVAVNPHARLARMAIRRGWA
                     IEDWG"
BASE COUNT         1493 a         3524 c         3648 g         1680 t
ORIGIN      
        1 agctcgtcgg cgacgatctg cggaagcgcg gtccacgcgc cctgcccggt ctccacctgc
       61 gggacggcga ccgtcacccg cccgtcgcgg ccgaccttga ggaaggggcc gaacgcccgc
      121 tcgcccttgc gctcgatcaa atcgcccgaa ggcctgcgcg gccaggcgag gatggcgacg
      181 acgaggccga tcccggcgcc cccgccgatc agcagcgtgc ggcggtcgac gggtgcgagc
      241 ccccgggtca acgcgcgtat tgggccttca gcgcgacccg gtcgatcttg ccggtgccga
      301 ggcgcggcag cggctctttg gaaaaaatca tccgcgccgg caccttgaac gcggccagcc
      361 gcgattcgag gaaagcgcgc aggccggctt cgtccaaatc gctgccgtcc gccggatgga
      421 tgatggcgac cggcacctcg ccgagccgct cgtcggggac tccgaacacc gcgcattcat
      481 tgacctcggc atgctcgtag atcgccgcct cgacttcctg gcagctgata ttctcgccgc
      541 cgcggatgat gatgtccttc ttgcggtcga cgatgaacag ataatcgtcc tcgtcgaaat
      601 agccgatgtc gccggtgcgc acgtagccgt cggcggtgaa cagcgcctgc gtcgcggccg
      661 ggttgcgcca atagcccttg atgttggcgg ccgagcggat ggcgatctcg ccgcgctggc
      721 cgggcggcag gtgcgtgtcg ttcgcgccga ggatcgcgac ctcgacgaac ggcctggccg
      781 gccgcccggt cgaggccggc ttggcggcgt agctgcccca gaaattgatg cagccgacgg
      841 cgttggtctc ggtcagtccg tagccgagcg ccggctgggc gtcggggaag gcgtccttca
      901 accgctcgac atggctgacc ggccgcggcg caccgccggc ggtgatgtcc ttcaaggacg
      961 acagatcgta gcggtcgcgg tcgggatggt tgagcagctc gaggctcatg gtcggcacgc
     1021 cgacgaaata ggtgatcttt tccttttcga tcagcctgag cgcctcggtc gcgtcccatt
     1081 tgggcatcac caccatgcag cggccgacga cgaagctgtt gagcatcacc ggcacctcgc
     1141 cggtgacgtg gaacaagggc acgctcaaga gggtgcgcgg cgggcccgcg ggctcgcggc
     1201 cgtcctcgat cagaagcccg agcagggtca tcagcccgct cgaataagtg taggtgccgg
     1261 tggtcacctg gcggtgggtc gacaaggcgc ccttggcctc gccggtcgag cccgaggtga
     1321 acagcagggt cgcgtcgtcc tcgggcgcga tctcgggcag cgccgcggcg gcgcccgaac
     1381 cggccagcag cgtcgcaagc gcggtttcga gcggctgctc gaccgccagg gtgacgacgt
     1441 cgtggttccc gccgaccgac tcgatccgtt tcgagcgcgg ctcgtcggcg atcaccagcc
     1501 cgggctcggc cagggccagc gcgtgggcga gctcgtggct ttgccaccag ccgttgagca
     1561 gcgtcgcaac cccgccggcc ttgaggatcg ccatgtaggc gacgatccag ctcgggcagt
     1621 tgcgcatcgc gatcccgacg cggtcgccct tggatatgcc ccggccggcg agcgccaagg
     1681 cgaggctttc cgacacccgg tcgaggtcgg cgaaggtcag ccgctcgtcg ccggcgacca
     1741 gcgcctcgcg ctcgtcgccg tagagcgcgc agaaggtgcg cagcagcccg ggcacggtcg
     1801 ccgggaagtt gcccacgatc gcccgcccct gcgaatcgcg ctcgatcacc agccgcccgc
     1861 cggggccggt gacccgggcg agcatgtcgt cgaacctgcg gtcgagatcg ctcggcatat
     1921 gcggctgaac gctcctttgc cctttgcggc agcccatctt atgaggcgct gaacctcaag
     1981 ggggaagtcc ttgcaacccg tcaatgccga gcccgacgca atcgcctgga ccgaccttca
     2041 cctgtcgccg gtcgcgctcg acctcggcct gttcgagctc cgatggtaca gcctcgccta
     2101 cctcgccggg attttcatcg gctactggta tttgctcagg ctccttaagg agccgggcgc
     2161 gccgatggcc cggaggcatg ccgacgacct ggtgttctac gcggccttgg ggatcatcct
     2221 cggcggccgg ctcggctacg tcctgttcta caaccttggc tattaccttc agaacccggt
     2281 cgacatcctg aagctgtggg atggcggcat gtcgttccac ggcggcgtga tcgggacgac
     2341 ggtcgggatc ctctatctgg cgcgcaagga gaaactgccg tggctcagga tccacgacta
     2401 tgtcgcctgt tgcgtcccgt tcgggctgtt cttcggccgg ctcgccaatt tcgtgaacca
     2461 tgaattgtgg ggcgccgagg cgagcgtgcc gtgggcggtg cggttccccg aggtcgtcaa
     2521 cggtgtcacc atcctcggcc cgccgcgcca cccgacccag ctctacgagg cgctgctcga
     2581 gggcgtcgtc ctgttcgcga tcctgtggtg gatgttccgg aaaaccgagg cccgctacca
     2641 gccgggcaag ctcgtcggag cgttcctgtt cttctacggg ctgttccgct tcgcggtcga
     2701 gttcgtccgc gagcccgatt cgcatttggt cgaattcgcc caggcgagcg ggctgcacat
     2761 gggccagtgg ctgtcgctgc cgatgatcgc cggcgggctc tacctgatga tgaccgcggc
     2821 ccggcggcgg gtccgggtcg agccgaccct gggtccggcg agcgtcgcct gagcgggttc
     2881 cgcgacgagc ttttcgcgat gatccgcgcc ggcggcccga tcagcgtcga gcgctacatg
     2941 ggcctcgcca acgcgcatta ttacgcgacc cgcgacccgc ttggcgtggc cggggatttc
     3001 accaccgcgc ccgaggtcag ccagatgttc ggcgaattgg tcggggcggc gctcgccgat
     3061 tgctggcggc gcgcgggggc gccggccgat gcagcctatg ccgagctcgg cccggggcgc
     3121 gggacgctcg cggccgacgc gctgcgggtg ttgggcaagg cggggttcgc gggcgaggtg
     3181 catctcgtcg agaccagccc gccgctgcgt gccgagcagg cgaagcgggt gccgggcgcg
     3241 cgctggcacg acagtgtgga ggaattgccg ccgcgcccgg tcctgctggt cgccaacgag
     3301 ttcttcgacg ccttgccggt gcggcaatgg gtcggcggcg aggagcgtcg ggtagcggtg
     3361 gccggcgatg cgctggtttt cactgtcgat gggcccatcc gcgaggattc gccggcgcgc
     3421 gaggaagcgg tccgagcggt tgcccggcat ctcgccgagc acggcggagt ggcgctggtc
     3481 atcgactacg gccacgctcg gtcgggccaa ggcgacacct tgcaggcggt gcgcggccac
     3541 gcctttgccg acgtgctggc cgacccgggc gagcaggatt tgaccgcgca cgtcgatttc
     3601 gaggcgcttg ggaggacggc gtcggcggcg aaagtgaccc gggtcgtgag ccagggcgaa
     3661 tggctcgagc ggctcggaat tgccgcgcgc gccgagtccc tcgccgaggt gcacccggag
     3721 cgcgcggacg agttcgcagc ggcgcgcaag cggctgtgcg acccggacga gatgggcgcg
     3781 ctgttcaagg tgatggcgat ccattcgcgc tcgtggccgt ccccggcggt gttcgaatga
     3841 gcatcgccca ccgaatggcg agcgaagccg acctgccggc gatcgaccgg gtcttccgaa
     3901 ccggcttctg cgacacgttc ggccatttgt atcgcagcga ggatctcgac gcatttctca
     3961 gccagttttc gccgcaggcc tggctcgccg agcatcgcga cccgagctat gcgttccgga
     4021 tcgccgaagc acgcggggac gcggtggcct tcgccaagct cgggtcgctg agcgtgccgg
     4081 tcgagccggc cgggccggcg ctcgagctgc gccagctcta cgtgctgcgg ccatggcaag
     4141 ggaccggcat cgcgcaacag ctgatggaat gggcgctggc cgaggcgcgc gcccgcggca
     4201 tcgccgagct gtacctgacc gtctacaccg acaatcaccg cgcccgcgcc ttttaccggc
     4261 gttacggatt cggggacgtc ggcccctacg cattcatggt cggcagccac gccgacgagg
     4321 acatcatcat gcggctggcg ctttgagccc ggaggtgagc gtcgaagtcc tgcgcgccgc
     4381 gaacctcgcc ggagccccgc acggcttcct cggccggcgc ggcggagtgt cgaccggcga
     4441 gctcgagggc ctcaatgtcg gctacggcag cggcgacgac cgcgacgcga ttgcgcagaa
     4501 ccgggcgcgg gcgatcgccg cggtgcttcc gggcgccggg ctggcgacag tccaccaggt
     4561 gcattcgccg accgcggtcg tggccgagcg gccgtggccg cacgacgagc ggccgcgcgc
     4621 cgacggactg gccaccgacc ggccggggct gctgctcggg atcctgaccg ccgactgcgc
     4681 gccggtgctg ctcgccgatc cggccgccgg agtggtcggc gccgcccatg ccggctggcg
     4741 cggggcgctg gccggggtga ccgacgagac gatcgcggcg atggagcggc tgggcgcgcg
     4801 gcgcgagcgg atcgtggcgg cggtcgggcc gtgcatcggg cgcgattcct accaggtcga
     4861 cgagacgttc cgcggccgct tcctcgggct cgacccggcc aatgaccgct ttttcagcgg
     4921 cggccccggc aaccggccgc acttcgacct cgccgcctac gtcgccgccc ggctggaagc
     4981 ggccggcatc gccggggtcg aggcgctcgc gctcgacacc tatgccgagc ccgagcgctt
     5041 cttcagctac cgccgatcga cccaccgcaa ggaggcggac tacgggcggc agatcgcgat
     5101 gatcgggctc gctccatgag cgcgggcaat tcgaccgcgc gctggggtct gtggggcgga
     5161 ttgctggcct tcgtcgtgct gctggcgctg ccggcgcccg cggagatgcc gctgccggcg
     5221 tggcggacga ccgctctggt ggtgctgatg gcgacctggt ggatgaccca agcgctgccg
     5281 ctgacggcga ccgctttgct gccgttcgtc accttcccct tgttcgggat catggacgcc
     5341 aacgcggccg ccgcgtctta ttattcgccg atcctgttcc tggtgctggg cggggcgatc
     5401 atcgccctgg cgatcgagcg cacccagctc caccgccggc tggcgctggc gatcgtccag
     5461 cgcggcggga gcaccccggc ggcgatgctg ctggcgttca tgaccgcgac cgcgatcatc
     5521 tcgatgatcg tctccaacac cgcgacgacg ctgatcatga tgccgatcgc gctcgccgtg
     5581 ctagccgcgg cgcaggtcaa gcccgggcac agcgacgggt tcgccggcgc gttggcgatg
     5641 ggcatcgcct tcgccgcgtc gatcggcggg ctcgggaccc tggtcgggtc gccgaccaac
     5701 gccatcgccg ccggcatcat cgagcgcgcg acgggtctcc acatcggctt cctgacctgg
     5761 gcgatgtacg gggtcccgct ggtgctggcg gcgatcccgg ccgcctggtg gatattgatg
     5821 aaggtgcagc gggtgcggcc gaccgacttc gacccggccc aggcgctcgc cgggatcggc
     5881 accgcgggcc aatggagcgc cgccgagaaa aggctggtgc cgctgatcgc cgcggtggtc
     5941 gcgggctggg tggcgatccc gttcgtgacc ccgttcctgc cgaaggattc gctgaccgac
     6001 gggacgatcg cgatcttggg cgcgctgctc ttgttcgtga tccccgacgg cagcgccgag
     6061 ggtggcaaga ggcgggcgat cctcacctgg aaggaagcca accgggcgcc gtgggacgtg
     6121 atcatgctgt tcggcggcgg cctcgccctg gccgcgggga tgggccagtc ggggctcggc
     6181 gactggctcg gcgtcgcctt gcagccgctt cgcgaggtcc atcccctggt ggtcgcggtg
     6241 gtgctggtgg cgatcgtcat cgtcatcacc gagttcgcat cgaacgtcgc cgcggcgagc
     6301 ggcatcatcc cggtggtcgc cgggatcatc gccgcgaccg ggatcgaccc gatcctgctg
     6361 gcgctgccgg ccgcgtgggc ggcgagctgg ggcttcatgc tgccgtcggg gaccgggccc
     6421 aacgcgatcg cctgggcgac cggccacatc gccttgccgc ggatgctcaa ggccgggctc
     6481 ctgatcgacc tcgccggggt tccgctgatg gtcggaatcg tgtggctgat cgcggccgcg
     6541 atgggctaga gccggccaat ggcgcacgaa gaacccgaca cggacgaatc cgaactgagc
     6601 ggcgcgaccc cgcggcgcaa gcccaagccg tcggcgactc gcatcggcga ccataaattg
     6661 agcgcctcga cgctgatgat gggctacggc ttcgatcccg cgctgagcga gggctcgctg
     6721 aagccgccgg tgttcctgac ctcgaccttc gtgttcgaga acgccgcggc cgggaagcgc
     6781 tttttcgagg gcgtcaccgg caagcgcccg ggcggcgccg aagggctgat ctactcgcgc
     6841 ttcaacggcc ccaaccagga aatcctcgaa gaccggctga gcatctggga ggaagccgac
     6901 gacgctctgt ccttctcgag cgggatggcg gcgatcgcga ccctgctgct gaccttcgtc
     6961 cagcccggcg acgtggtcgt gcactccggc ccgctctacg cggcgaccga gaccctgatc
     7021 gccaagatcc tcggccgctt cggggtgtcg tggctcgact tcccggccgg agcgacgcgc
     7081 gaggagatcg actcggtgat cgccgaggcg aaggccaagg ggcgggtgtc gctgatctac
     7141 ctcgaaagcc cggccaaccc gaccaacgct ttggtcgatg tcgaggcggt cgcggcggct
     7201 cggaatgccg cattcggccc gaacgagcgg ccgccgatcg cgatcgacaa caccttcctg
     7261 gggccgctgt gggcgcaccc gctcaagcag ggcgccgacc tcaccatcta cagcctgacc
     7321 aaatatgccg gcgggcacag cgacctcgtc gccggcgggc tggtcgggcg ccaggcgctc
     7381 atcgacccgg tgcggatgat gcgcaacacc atcggcacca tctgcgaccc gaacaccgcc
     7441 tggatgctct tgcgcagcct cgagacgctc gaattgcgaa tggagcgcgc cggcgacaat
     7501 gcggccaagg tgtgcgcgtt cctgcgcgac cacccgaagg tcgagcaggt cggctacctc
     7561 ggcttcctcg agccgggctc gcgccaggcg gacatctacg cccgccactg caccggcgcc
     7621 ggctcgacct tctcgctcta tttgaagggc ggcgagaagg aagcgttcgc gtttctggac
     7681 gcgctgacca tcgcccacct cgcggtcagc ctcggcggca ccgagacgct ggccagcgcc
     7741 cccgcggcga tgacccattt gtcggtcccc gacgatcgca agcaggcgct ggcgatcacc
     7801 gacaacctgg tgcgaatctc gatcggggtc gaggacgcgg acgatttgat cgccgacttc
     7861 gagaacgcgc tgaaggcggt ctagacgggc gccagtcgcg gccgacggct agtcgccgcc
     7921 aggctaatgc ccggcggcct gggccgcggc ggtgtccgcc tcctcgtggg cgcggaagat
     7981 ctcggcccgc tgctcgcccg agacctcgag cagtcccttg aggcctttct ccagccgcga
     8041 cagcgcgtgg tcgacgagga tgaaacggcc cggcacctcc accctcaggt cggtgacggc
     8101 cgcgccgccc ggcggcacgg tgatggtctg aacatcgctc atcggcgccg acgtgaccga
     8161 ggccagcggg taggccttgt cgaaaatctc gccgatgacg tggaagctcg acgtcttgtt
     8221 gggcccgccg acgccgaaga agacccggac ggattcgccg acctttgcct tgagcggctt
     8281 cctcgagagc ccgtccgcgg cgccgttgaa gaccagatat tcgggcgttt cggcgagcaa
     8341 tttgtcgatg ctctcggtga gctcgccctt cgttccgttc ggctgctcgg tgtagatctc
     8401 gccctgcatg acatagaatt cgcgatcgac cttggagagg ccctcctggg gctcgacgag
     8461 gatcaggccg tacatgccgt tggcgatgtg ctgggcgacg atcggggtcg cgcaatggta
     8521 gacgtacagg ccggggttca gcgccttgaa ggtgaagccc ttggtctcgc ccggggccgc
     8581 catagtggcg attccgccgc cgcccgggcc ggtgaccgca tgaaagtcga cattgtgcgc
     8641 catcacgctg tcgccggcgt tcttcatcgt cacttcgacg gtgtcgccga cccgcacccg
     8701 gacgaacggg ccaggcacct tgccgttgta ggtccagtag cgataggtcg agccgtcatc
     8761 gagccggccg gtcacctcga cggtgtcgag gtggaccttg accagctgcg ggccgcggac
     8821 cgcgagcggg cccggcaggt cggcggggtc gcggacgatg tcggccgccg gcgccgcggc
     8881 cggagcggcg gcgacttcat tgtccgctgc cagagcgggg gccgacgcca ggagcgctcc
     8941 gagcgccgcc gctgcaatcc gcagccggtt tccagctggt ttcacaaatc acctccgtcc
     9001 gacatcaggg tggccggcaa gctccggccg ggcacgtcac ctcgcggcga ttcgaaagcc
     9061 gctgcttgcg aataatgttg cgaaggcctg cttcgccttg ccggggtggc tgaccttctt
     9121 ggccgcctcg agattggagc tcgcattgcc gaccacgacg acgccgacca tcccgacgcc
     9181 gtaatgcggc aggcacttgt agccgtacac gccgggcttg gtgaatttga cgctgacgtt
     9241 ctggttcatc ttgcccttga acggcgccgc cccggccggg agcatgccgg cgatgctttc
     9301 ggcattgtgg gtcttgtcgg tggccacgaa cgtgacggtg tcgcccggcg cgaccttgat
     9361 cagcgccggc tcgaagacca tcggcccgga ctggcccttg ttgagcatct tgacgacatg
     9421 atccttggcc gccgcggggc ttgtcgcggc gagggcgaca gcagccccca ggaacaggaa
     9481 tggaattcgc atgaccgtct ccgtgattca ctattttcga tgactgatgg tcacggagta
     9541 catttcggtc cgccggcgcg tgaatacgga aacctaccta ggctggcggc ggcttcagcc
     9601 ttgcgaaccg cgcccgcgac cgggcgcgga atcgggcggg gaacgcccta gccccagtcc
     9661 tcgatcgccc agccgcgcct gatcgccatg cgggccaacc gggcgtgcgg gttgaccgcg
     9721 accggctcgt cgacccattc gaaagtcggc gcgtcggacg cgtggtcgga gtagaagcgg
     9781 acgtggccgt ggcggccggt gaggccggac ttctcgaccc attcggcgat catccgcatc
     9841 ttttccgcgc catagcaatt cttgccggcg atccgcgcat ggacgcgctc gtcgaggccg
     9901 atcaccgagc cggtggcgat gacgtcgtcg aagccaagcc gctcggcgat cgagtccgcg
     9961 tagagccggt aagacgcggt cgccagcacc agccggcgcc cctcttcgcg gtcgcgttcg
    10021 atcgcccgcc tcgcgccggg ccggatattg tgctgcaccg tggcttccgc gaagctgtcg
    10081 gccagcggct tcagctcggc ggcgcggacc ctgcggccga ccagcagcgc ctggcagatt
    10141 tccttcagcc gcccgcggtc gaacaggccc atcgcatagg caagcatcgc cagcaggacg
    10201 aagggcacga acaagagtcg ccacggctgc cgccgcatcg cgcaatgggt gaggaacagc
    10261 gtgtaggtcg gtcgccgggt gatcgtccgg tcgagatcgt agacggcgag gtcagccatg
    10321 ggtgcgctcc gcaagcagcg cttcc
//
LOCUS       DASTZO010000199         4811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_251928,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000199 DASTZO010000000
VERSION     DASTZO010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251928"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..1202
                     /gene="mraY"
                     /locus_tag="VFU20_05515"
     CDS             132..1202
                     /gene="mraY"
                     /locus_tag="VFU20_05515"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU5285953.1"
                     /translation="MLYLIAEELGFPGLLNLIRYISFRAGAASATALAIGLLLGPWFI
                     SYLRTRQGKGQPIRSDGPQSHLAKSGTPTMGGLLILISVSVSVLLWMDLGNPYVWACL
                     LVTAGFGLIGFLDDYDKLKKAHHAGLSGRMRLALEFLIAGFATWLIVRAGTSSLYVPF
                     VQGPVMDLGWFYIAFGAFVIVAFGNAVNLTDGLDGLATMPVIIASVAFLLIAYLVGNA
                     RFAEYLGIPHLLGVGDLTVLCLAIVGAGLAFLWFNAPPAAVFMGDTGSLALGGAIGAI
                     AVATHHEFVLVIIGGLFVLEAASVVIQVMVYKRTGKRIFRMAPIHHHFEHIGWSEPTI
                     VIRFWIVSFVLALAGLSTLKLR"
     gene            1199..2506
                     /gene="murD"
                     /locus_tag="VFU20_05520"
     CDS             1199..2506
                     /gene="murD"
                     /locus_tag="VFU20_05520"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019051520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU5285954.1"
                     /translation="MIAARAFADRHYALYGLARSGLATVEALLASGAKVTAWDDSEEA
                     RAKAPEGAELEDFDTADLARFDSLVVSPGVPLNRHPIAERARDAGVEIIGDIELFARA
                     RPQLPAHRVVGITGTNGKSTTTALIHHILKTAGVPSAMGGNIGLPILAQDPLAPNDKG
                     VGVYVLELSSYQLDLTQSLDCDVAVLLNITPDHLDRYEGFEAYAGSKARLFAMQTRGR
                     VGIIAEDDEPTREIGAKLGRDDYWRRNKLVCVSRIDVGLQANLPTLQGPHNAQNIACA
                     VATCRHLGVSYATIVAGLETYPGLPHRMERVRDKDGVVFVNDSKATNPTAAAPALAAF
                     TGIRWICGGQAKSDSLDECEPHFGHVAKAYTIGEAAELFERLLKPHMPVKNCRELEAA
                     VKAAAQDAQSGDTVLLSPACASFDQFRDFEERGDRFRECVGAL"
     gene            2503..3723
                     /locus_tag="VFU20_05525"
     CDS             2503..3723
                     /locus_tag="VFU20_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEU5285955.1"
                     /translation="MSTSFSSRIKAKAAWADPSRYGRSDRSAAGRWFWEIDRVLLLLV
                     SVLIGIGLIAVAAASPAAATRYSGGSVQFGELYYFYRQLAWIAVGVPVMIAISMLTRE
                     RARRLSLFGAAFFFVLLIFVPILGPEINGAKRWLEIGVGQLQPSEFLKPFYVVAMAWL
                     LSLREGDKSLPVFALSALITGAVAFLLMRQPDFGSTIIFGCVWIAMLALAGVSLRALG
                     VLGLTVVAGVVLAYFTYDVATARIDGFLFGEGDTFQTDNAMRTLTAGGLFGMGPGGGT
                     RKFGLPEPHTDYIFSVIGEEFGLIACLAIALLYLTIVVRVLVKLLDEDSTFAILAAAG
                     LVITFGLQAMINMAVNVQIAPSKGMTLPFISYGGSSMLALSIGMGLLLAFTRRNPYLT
                     RSPYVVKWSGDCAQ"
     gene            3776..>4811
                     /gene="murG"
                     /locus_tag="VFU20_05530"
     CDS             3776..>4811
                     /gene="murG"
                     /locus_tag="VFU20_05530"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEU5285956.1"
                     /translation="MIPAHALAAELKSRGHGVMLVTDERGARFPGLFDKVPVHILPAG
                     RLGGGPIGWLKALGSVIAGRGEARGLYRNHRPDAVVGFGGYPAFPSLLAASSMGIPSL
                     LHEQNAVLGRVNRLLAGDAATIATAFEKVERLKPRHRNKIALVGNPVREEIARLGEMP
                     FPAFDEVAPLKILITGGSQGASVLGKVVPEGLGLLKPSLRRRLQIIQQCRADDIDRVR
                     ARYAELGIPAELMTYIEDMPDKLAEAHLVIGRAGASTIAELTAAGRPAILIPFPSATD
                     DHQTANAREMTRAGGARTIPQTDFTPQVLARQIEAMAADPQALANAAARSLSVGRPHA
                     ARDLADLVERV"
BASE COUNT          695 a         1665 c         1633 g          818 t
ORIGIN      
        1 cgcgccgcca gcgtcgacga ggcgaccgag ctgctgctgc ggcttgcccg gggcggtgac
       61 gcggtgctgg tcaaggcgtc gaactcggtc gggcttgcga ggctggtcga acgggtgtca
      121 gggcttcagc catgctctac ctgatcgccg aggagctcgg cttcccgggc ctcctcaacc
      181 tgatccgcta catcagcttc cgcgccggtg cggcgagcgc gacggcgctg gcgatcgggc
      241 tcctgctcgg cccctggttc atctcctacc tgcgcacccg ccagggcaag ggccagccga
      301 tccgctccga cggcccgcaa agccacctcg ccaagagcgg caccccgacc atgggcggcc
      361 tcctgatcct gatctcggtg tcggtctcgg tgctgctgtg gatggacctg ggcaacccct
      421 atgtgtgggc gtgcctgctg gtcaccgcgg ggttcggtct gatcggcttc ctcgacgatt
      481 acgacaagct caagaaagcg caccatgccg ggctttcggg ccggatgcgc ctcgcgctcg
      541 agttcctgat cgccggcttc gcgacctggc tgatcgtccg agccggcacg tcgagcctct
      601 acgtgccgtt cgtccagggg ccggtgatgg acctcggctg gttctacatc gccttcgggg
      661 cgttcgtgat cgtcgccttc ggcaatgcgg tcaatctcac cgacgggctc gacggcctcg
      721 cgaccatgcc ggtgatcatc gcctcggtcg ccttcctgct gatcgcctac ctggtcggca
      781 acgcgcgctt cgccgaatat ctcggcatcc cgcatttgct cggggtcggc gatctgaccg
      841 tgctgtgcct ggcgatcgtc ggcgcgggcc tcgcgttcct gtggttcaac gcaccgccgg
      901 ccgcggtctt catgggcgac accggcagcc tcgccctggg cggggcgatc ggagcgatcg
      961 cggtcgccac ccatcacgag ttcgtgctgg tgatcatcgg cgggctgttt gtgctcgagg
     1021 cggcgtcggt tgtgatccag gtgatggtct acaagcgaac cggcaagcgc atcttccgga
     1081 tggccccgat ccaccaccat ttcgagcata tcggctggtc cgagccaacc atcgtcatcc
     1141 gcttctggat cgtcagcttc gtgctggcgc ttgccggcct cagcacgctg aagctcaggt
     1201 gatcgccgcg cgcgccttcg cggacaggca ttacgcgctg tacgggctcg cgcgctcggg
     1261 gctggcgacg gtggaggcgc tgcttgcgag cggggcgaaa gtgaccgcgt gggacgacag
     1321 cgaggaggct cgggcgaagg cgccggaagg cgctgagctc gaggacttcg acaccgcgga
     1381 cctggcccgg ttcgacagcc tggtcgtgtc gcccggcgtg ccgctgaacc gccatccgat
     1441 cgccgagcgc gcgcgcgatg ccggcgtcga gatcatcggc gacatcgagc tgttcgcccg
     1501 cgcccggccc cagctgccgg cgcaccgggt ggtcggcatc accggcacca acggcaagtc
     1561 gacgaccacc gcgctcatcc accacatcct caagaccgcc ggagtcccga gcgcgatggg
     1621 cggcaacatc gggctgccga tcctcgccca ggatccgctc gcccccaatg acaaaggcgt
     1681 cggcgtctat gtgctcgagc tgtcgagcta ccagctcgat ctgacccaga gcctcgactg
     1741 tgacgtcgcg gtgctgctca acatcacgcc ggatcacctc gaccggtacg agggcttcga
     1801 ggcctatgcg ggctcgaagg cccgcctgtt cgcgatgcag acccgcggcc gggtcggcat
     1861 catcgccgaa gacgacgagc cgacccggga gatcggcgcg aagctcggcc gcgacgacta
     1921 ttggcggagg aacaagctag tctgcgtcag caggatcgac gtcggcctgc aggccaatct
     1981 cccgaccctg cagggcccgc acaatgccca gaacatcgcc tgcgcggtcg cgacgtgtcg
     2041 gcatctgggc gtgtcctacg cgacgatcgt cgcggggctc gaaacctatc ccgggctgcc
     2101 gcaccggatg gagcgcgtgc gcgacaagga cggggtcgtc ttcgtcaacg acagcaaggc
     2161 gaccaacccg accgcggcgg cgcccgcgct ggcggcgttc acgggcattc gctggatctg
     2221 cggcgggcag gcgaagagcg actctctcga cgagtgcgag ccgcacttcg gccatgtcgc
     2281 caaggcctac accatcggcg aggcggccga gctgttcgag cggctgctga aaccgcacat
     2341 gccagtgaaa aattgtcgcg agcttgaggc ggcggtgaaa gccgccgcac aagatgcgca
     2401 aagcggggac acggtgctgc tttcgccggc gtgcgcgagc ttcgaccagt tccgcgattt
     2461 cgaggagcgc ggcgacaggt tccgcgaatg tgtgggggcg ttatgagcac cagcttttcc
     2521 agccggatca aggccaaggc cgcgtgggcc gacccgagcc gctacggccg ctcggaccgc
     2581 tcggccgcgg ggcgctggtt ctgggagatc gaccgcgtcc tgctgctgct ggtcagcgtc
     2641 ctgatcggga tcggcctgat cgccgtcgcg gccgcctcgc cggcggcggc gacacgctat
     2701 tcgggcgggt cggtgcagtt cggcgagctc tattatttct accggcagct cgcctggatc
     2761 gcggtcggtg tcccggtgat gatcgccatc tcgatgctga cccgcgagcg ggcgcggcgg
     2821 ttgtcgctgt tcggagcggc attctttttc gtcctgctga tcttcgtccc gatcctcggc
     2881 cccgaaatca acggcgccaa gcgctggctc gagatcggcg tcggccagct gcagccgtcg
     2941 gaattcctga agccgttcta cgtcgtcgcc atggcctggc tgctatcctt gcgcgaaggc
     3001 gacaaatcgc tcccggtgtt cgccctgtcg gcgctgatca ccggcgcggt cgctttcctg
     3061 ctgatgcgcc agcccgattt cgggagcacg atcatcttcg gctgcgtgtg gatcgcgatg
     3121 ctggctctgg ccggggtcag cctgcgcgcc ctgggagtgc tcgggctcac ggtggtcgcg
     3181 ggggtggtgc tcgcttactt cacctacgac gtcgcgaccg cgcggatcga cggattcctg
     3241 ttcggggaag gcgacacctt ccagaccgat aatgcgatgc gcacgctgac cgccggcggg
     3301 ctgttcggga tggggccggg cggcggcacc cgcaagttcg gcttgcccga gccgcacacc
     3361 gactacatct tctcggtgat cggcgaggag ttcgggctga tcgcctgcct ggcgatcgcg
     3421 ctcctctacc tgaccatcgt cgtcagagtg ctggtcaagc tgctcgacga ggattcgacc
     3481 ttcgccatcc tcgccgccgc cgggctggtg atcaccttcg ggctccaggc gatgatcaac
     3541 atggcggtca acgtccagat cgccccgtcc aagggcatga cgctgccgtt catcagctat
     3601 ggcggaagct cgatgctggc gctgtcgatc ggcatgggtt tgctgctcgc tttcacccgc
     3661 cgaaacccgt atctcactcg ctcgccctac gtcgtcaaat ggagcgggga ttgcgctcaa
     3721 taaagctatg gccgggatga atttcgttct cgctgccggg gggaccggcg gacacatgat
     3781 cccggcccat gcgcttgccg ccgagctcaa gtcccgcggc cacggcgtca tgctggtcac
     3841 cgacgagcgc ggcgcccgct tcccggggct gttcgacaag gtgccggtcc acatcctgcc
     3901 cgccgggcgg ctgggcggcg ggccgatcgg ctggctgaag gcgctcgggt cggtgatcgc
     3961 cgggcgcggc gaggcgcggg gcctctaccg caaccaccgc cccgacgcgg tggtcggctt
     4021 cggcggctat ccggcgttcc cgtcgctgct cgccgcctcg tcgatgggca ttccgagcct
     4081 gctccacgag cagaatgcgg tgctcggccg ggtcaaccgg ctgctcgccg gcgacgcggc
     4141 cacgatcgcc accgccttcg agaaggtcga gcggctcaag ccgcgccacc ggaacaagat
     4201 cgcgctggtc ggcaacccgg tccgcgagga gatcgcccgg ctcggcgaaa tgcccttccc
     4261 ggcattcgac gaggtcgctc cgctcaagat tctgatcacc ggcggcagcc agggcgcgag
     4321 cgtgctcggc aaggtggtcc cggaagggct ggggctgttg aagccgtcgc tccgccggcg
     4381 cctgcagatc atccagcagt gccgcgccga cgatatcgac agggtccgcg cccgctatgc
     4441 cgagctcggc atcccggccg agctgatgac ctatatcgag gacatgcccg acaagctcgc
     4501 cgaggcgcac ctggtgatcg gccgcgccgg cgcctcgacc atcgccgagc tgaccgcggc
     4561 gggccgcccg gcgatcctga tcccgttccc ttcggcgacc gacgaccacc agaccgccaa
     4621 cgcgcgcgaa atgaccaggg cgggcggcgc ccggaccatc ccgcagaccg atttcacgcc
     4681 gcaggtgctg gcccgccaga tcgaggcgat ggcggccgac ccgcaggcgc tggccaacgc
     4741 cgcggctcgg tcgctgtcgg tcggccgccc gcacgccgcg cgcgacctcg ccgacctggt
     4801 cgagcgggtc g
//
LOCUS       DASTZO010000200         7682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_668415,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000200 DASTZO010000000
VERSION     DASTZO010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7682
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_668415"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VFU20_05535"
     CDS             <1..1220
                     /locus_tag="VFU20_05535"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5285957.1"
                     /translation="GALSVLGKIPSSALLGPFRDENIAFILLKFRRSAEAEPYAQKAI
                     ATAGAREARLRLALADGFLAAGDRKRALAMVGELGTETGAARRRILAGKRTGQAIDEP
                     AKAYAELLLGMAVDLNRLDNRSLPVAMVQVARYADPRNAAGAVLLALLLDGRGRVAEA
                     LEVLRSVPAADPLAPQARDTEARILVDEKRFDEALRLARSAASAPGASVSDLARLGDV
                     FAGMKRYNEAAGAYARAIGMARAQGLKDELWPLYLLQASALEDADRWPETKQALHAAL
                     ALAPEQPLVLNFLGYAKLERGEDLDAAEAMIRKASALAPDDASITDSLGWALYKRGRY
                     ADAIEALQRAAARDPGQPEIHEHLGDALYASGRRYEARFAWNAALVTAEEEIAARVRL
                     KLESGLTPATAAP"
     gene            1217..2032
                     /locus_tag="VFU20_05540"
     CDS             1217..2032
                     /locus_tag="VFU20_05540"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEU5285958.1"
                     /translation="MSGRKDSILAETACAKLNLALHVRGRRADGYHDIETVFAFCEDG
                     DELSGEAAGELTLAISGPFATDVPEDNLVIRAASTLRKAAGSAAGARLHLTKNLPVAS
                     GIGGGSADAAAALRLLARLWDLDPALAEGVAPALGADVPACLRSQSCRGTGAGDELEP
                     VDLGLAGTPVLLVNPRIPLSTADVFGRWDGVDHGPLGDWREGRNDLEPAAIALVPEIA
                     VVLDWLRKQPGAEFVRMSGSGATCFALFGGEAERGEAAAAVPGQWWRLATRLR"
     gene            2043..3401
                     /locus_tag="VFU20_05545"
     CDS             2043..3401
                     /locus_tag="VFU20_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU5285959.1"
                     /translation="MTDGRPILTAADMAAAEQRAIGAGTSVEQLMERAGTALAEAAFR
                     FAGPRPALVLAGPGNNGGDGYVAAQHLAARGVPVRIAALAEPRTEAARAARHSHWQGR
                     VEALRPDTEPAPLLIDALFGTGLKRGLEAAASEQLARLCAAAVASVACDLPSGVDSDS
                     GAELSPVPDFDLTVTFGALKPAHRLMPAMRRCGRVVLADIGIAATGGWHEIARPDLPP
                     LDPMGHKYGRGMVHCVAGAMPGAIALAAAAAARAGAGYVRIEARQIIANVPAAVVQGQ
                     PGGCDDERIGAVLIGPGLGRDGGGLLDRALASGRPLVLDGDALRLVGDPGRLRGLDAI
                     LTPHEGEFGQLFAGLAGTKAERALAAARASGATIVFKGPDTLVASPDGRLGFAPPAPA
                     WLATAGTGDVLAGIAAAMQARGLTAFEAASAAVWVHGRAGEIAGPRMIADDLVAAIPA
                     VP"
     gene            3398..4570
                     /locus_tag="VFU20_05550"
     CDS             3398..4570
                     /locus_tag="VFU20_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEU5285960.1"
                     /translation="MSDPIVRIAARGDGVSASGRHVPFAVPGDTVLDDGTLEHGPHHQ
                     APPCRHFPECGGCQLQHSDDHAYRAYLVSRVAGALDQHGLSPDIREPYLSPPDSRRRA
                     SLRALTAGQGAVLGFNAERSHRIVDMRECHILRPELFALVAPLRALLAGLLKPRRPGE
                     ARLTLTDQGVDVVLGGIEAAGLEAAERLTEFCTRHRLARMSLDQGLGPETLYEPVPAT
                     VTLSGTPVPFPVGAFVQATADGEAALVAAVREAVGEAKRTADLFAGIGTFALALPGPV
                     YAAEASRDAVLALKRASPAIATEHRDLYRRPLDSSELANFEAVVLDPPRAGAAEQVAA
                     LASSSVLRIAYVSCNPATFARDARTLVDGGYALSWVRPVGQFRWSTHVELAAAFER"
     gene            complement(4567..5016)
                     /locus_tag="VFU20_05555"
     CDS             complement(4567..5016)
                     /locus_tag="VFU20_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HEU5285961.1"
                     /translation="MSYSPILAPVVALVAWSLVMMLWMVAVRLPAMNKAGISLSSAPP
                     GARGAQLDGVVDDRVQWKSHNYNHLMEQPTLFYAIAVTLALMDMGGGLNLWLAWGYVV
                     FRVLHSIVQATVNRLVYRTPLFFLASLCLIAMTIHAAARALHDNGLI"
     gene            complement(5072..6142)
                     /locus_tag="VFU20_05560"
     CDS             complement(5072..6142)
                     /locus_tag="VFU20_05560"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEU5285962.1"
                     /translation="MTTESLIDTAKVNDTRKAELLSSPVEHIDITSFDARPIVEAMGK
                     MSFTSRDLARATGIYNAMLDDPDCSIFLVIAGSTSAGGCMDLYAELVRSNMVDAIVAT
                     GAAIVDMDFFEALGHKHYQALEIPDDKTLRSLYIDRIYDTYIDEEALQQTDFTIGKIA
                     DGLEPRPYSSREFIREMGKWLTKNGKKDNSLVKLAFEHDVPIFCPAFTDSSAGFGLVK
                     HQADAMKRGGHYMTLDSIADFRELTDIKIKAGTTGLLMIGGGVPKNFAQDTVVCAEIL
                     GHEDVEMHKYAVQITVADVRDGACSSSTLQEACSWGKVDTAQEQMVFAEATSVLPLLA
                     SDAWHRGAWRNRAKRRFAKLFD"
     gene            complement(6258..7442)
                     /locus_tag="VFU20_05565"
     CDS             complement(6258..7442)
                     /locus_tag="VFU20_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HEU5285963.1"
                     /translation="MACAADLAKRRPVQPVTLLRPHAARRAARYFVEKFPGRSLYAVK
                     ANPSPDLLKILWDEGVTHYDVASIGEVRLVARTLPQAVICFMHPVKAEEAIAEAYFEH
                     NVRAFSLDSFDELDKILRSTATNGVAATDLYLLVRLQVSSEHARLSLASKFGAEPQDV
                     KALLFAARQAADALGICFHVGSQAMTPAAFSDAMDRVRDAIVEAAVTVDVVDVGGGFP
                     STYPGMEPPPLEAYFNVIHAGFEALPISYSAELWCEPGRALCAEYASVLVRVEKRRDA
                     ELYINDGAYGALFDAAHIGWRYPVRLLRESASKVRPMEFSFYGPTCDDLDRMAGPFEL
                     PGDVAAGDYIEIGMLGAYGCAMRTGFNGFGVEGVEIVDDEPMATLYGAVEERKRSKAV
                     KL"
BASE COUNT         1109 a         2713 c         2731 g         1129 t
ORIGIN      
        1 ccggcgcgct gtcggtgctg ggcaagattc cgtcgtcggc gctgctcggc ccgttccgcg
       61 acgagaacat cgccttcatc ctgctgaagt tccgccgctc ggcggaggcc gagccctatg
      121 cgcaaaaggc gatcgccacc gccggcgccc gcgaggcccg gctgcggctg gccctggccg
      181 acggcttcct cgccgccggc gaccgcaagc gcgcgctggc gatggtcggg gaactgggca
      241 ccgagaccgg cgccgcgcgg cggcgaatcc tcgccggcaa gcggaccggc caggcaatcg
      301 acgagccggc caaggcctat gccgagctgc tgctcgggat ggccgtcgac ctcaaccggc
      361 tcgacaaccg ctcgctgccg gtcgcgatgg tccaggtggc gcgctacgcc gatccgcgca
      421 acgccgccgg ggcggtgctg ctcgctttgc tgctcgacgg ccgcgggcgg gtcgcggaag
      481 cgctcgaggt gcttcggtcg gtcccggccg ccgacccgct cgctccccag gcgcgggaca
      541 cggaagcgcg gatcctggtc gacgagaagc gcttcgacga agcgttgcga ttggcccgct
      601 cggccgcctc ggcgcccggt gcgagcgtca gcgacctcgc tcgcctcggc gacgtgttcg
      661 ccggaatgaa gcgctacaac gaagccgccg gcgcttacgc ccgggcgatc gggatggcgc
      721 gcgcccaggg gctcaaggac gaactgtggc cgctctatct gctgcaggcg agcgcgctcg
      781 aggacgccga ccgctggccc gagaccaagc aggcgctcca tgcggcattg gcgctcgccc
      841 ccgagcagcc gctggtgctc aatttcctcg gctatgcgaa gctcgagcgc ggcgaggacc
      901 tcgacgccgc cgaggcgatg atccgcaagg cgagcgcgct cgcacccgac gacgcgtcga
      961 tcaccgattc gctgggctgg gcgctgtaca agcgcggccg ctacgccgac gccatcgagg
     1021 ccctccagcg cgcggccgcc agggaccccg ggcagcccga gatccacgag catctcggcg
     1081 acgcgctcta cgcgagcggc cggcgctacg aagcgcgctt cgcctggaac gccgccctgg
     1141 tcaccgccga ggaggagatc gccgcccggg tccggctgaa gctcgagagc ggcctgacgc
     1201 cggcgaccgc cgcgccttga gcgggcgcaa agacagcatt ctggccgaga ccgcctgcgc
     1261 gaagctcaac cttgcgctcc acgtccgcgg ccggcgcgcc gacggttatc acgacatcga
     1321 gacggtgttc gccttttgcg aagatgggga tgagctgagc ggcgaagccg ccggggagct
     1381 gacgctggcc atcagcgggc cgttcgcaac cgatgtgccc gaggacaatc tggtgatccg
     1441 ggcggcgagt acgctccgca aggcggccgg cagcgccgcc ggagctcggt tgcacctgac
     1501 caagaatctc ccggtcgctt cgggaatcgg cggcggctcg gccgatgcag cagccgcatt
     1561 gcgcctgctg gcccgcctct gggacctgga cccggcgctg gcggaggggg tcgcgcctgc
     1621 tctcggggcg gacgtgccgg cgtgcctgcg gtcgcaaagc tgccgcggca ccggcgccgg
     1681 ggatgaactc gagccggtcg acctcggcct cgccggaacg ccggtgctgc tcgtcaaccc
     1741 gcgcatcccc ttatcgaccg ccgacgtgtt cgggcgctgg gacggcgtcg accacgggcc
     1801 gcttggcgac tggcgggagg gccgcaacga cctcgagccg gccgccatcg cgctggtccc
     1861 cgagatcgcc gtggtgctcg actggctccg caagcagccc ggggccgaat tcgtccggat
     1921 gtcgggcagc ggcgcgacct gcttcgccct gttcggcggc gaggccgaac gcggcgaggc
     1981 ggccgcggcc gtgcccgggc aatggtggcg cctcgcgacc cgcctgcgct aagaggcgcg
     2041 ccgtgaccga cgggcgcccg atcctgaccg ctgccgacat ggctgccgcc gagcagcgag
     2101 cgatcggcgc cggcacctcg gtcgagcagt tgatggaacg agcggggacc gcgctcgccg
     2161 aagccgcttt ccgcttcgcc gggccgcgcc cggcgctggt gctggccggc ccgggcaaca
     2221 atggcggcga cggctatgtc gccgcccagc acctcgccgc gaggggcgtc ccggtgcgca
     2281 tcgccgcgct cgccgagccg cggaccgagg cggcccgggc ggcgcgccat tcgcactggc
     2341 agggcagggt cgaggcgctc aggcccgaca ccgagccggc gccgctgctg atcgacgcct
     2401 tgttcgggac cgggctcaag cgcggcctcg aggcagcggc gtccgaacag ctcgcgcgcc
     2461 tgtgcgcggc ggccgtcgcc agcgtcgcct gcgacctgcc gagcggtgtc gacagcgatt
     2521 cgggagccga gctcagcccc gtcccggact tcgacctgac ggtcaccttc ggcgccctca
     2581 agcccgccca ccggctgatg ccggcgatgc ggcgctgcgg ccgagtggtg ctggcggaca
     2641 tcggcatcgc ggcaaccggc ggctggcacg agattgcccg tcccgacctg ccgccgctcg
     2701 accccatggg ccacaaatac ggccgcggca tggtccattg cgtcgccggc gccatgcccg
     2761 gggcgatcgc gctcgccgcc gccgcggccg cccgcgccgg cgccggctat gtgcggatcg
     2821 aggcccggca gatcatcgcc aacgtccccg cggcggtggt ccagggccag cccggcggct
     2881 gcgacgacga gcgcatcggc gcggtgctga tcggcccggg cctcggccgc gacggcggcg
     2941 ggcttcttga ccgggcgctc gcgagcggcc gcccgctggt gctcgacggc gacgctctgc
     3001 ggctggtcgg cgaccccggg cgcctgcgcg ggctcgatgc gatcctgacc ccccacgagg
     3061 gcgagttcgg gcaattgttc gccggcctcg ccggaaccaa ggcggagcgt gcgctcgccg
     3121 ccgcccgggc atcgggcgca acgatcgtct tcaaggggcc cgatactctg gtcgcatcgc
     3181 ccgacggccg actcgggttc gcgccgccgg cgcccgcctg gctggccacc gccggcaccg
     3241 gcgacgtgct tgccgggatc gccgcggcga tgcaggcgcg cgggctgact gcattcgaag
     3301 ccgcctcggc cgcggtctgg gtccacggcc gggccggcga gatcgccggg cccaggatga
     3361 tcgccgacga cctggttgcc gcaatcccgg cggttccgtg agcgacccca tcgtgcgcat
     3421 cgccgcgcgt ggcgatggcg tctcggcaag cggccgccac gtgccgttcg cggtgcccgg
     3481 cgacacggtg ctcgacgacg gcactctcga gcatgggccg caccaccagg cgccgccgtg
     3541 ccgccacttc ccggagtgcg gcggctgcca gctgcagcat tccgacgacc atgcgtatcg
     3601 agcctatctg gtgtcgcgag tcgccggcgc gctcgaccag cacggtcttt cgccggacat
     3661 ccgcgagccg tatctgtcgc cgcccgacag ccgccgccgc gcgagcctgc gcgcgctcac
     3721 ggccgggcag ggggcggtgc tgggcttcaa cgccgagcgc tcgcaccgga tcgtcgacat
     3781 gcgcgaatgc cacatcctga ggcccgagct gttcgccctg gtcgcgccgc tccgggcgct
     3841 gctcgccggc ctgctgaagc cgcggcggcc cggcgaagcc cggctgacgc tcaccgacca
     3901 gggcgtcgac gtcgtccttg gcggaatcga agcggcgggg ctcgaggcgg ccgagcggct
     3961 gaccgaattc tgcacccggc accggctcgc ccgaatgagc ctcgaccagg gcctcgggcc
     4021 ggaaacactc tacgagccgg tgccggcgac cgtcaccctg tccggcacgc cggtcccgtt
     4081 cccggtcggc gctttcgtcc aggcgaccgc ggacggcgag gccgcgctgg tcgccgccgt
     4141 ccgcgaggcg gtcggcgagg cgaagcgcac cgccgatttg ttcgccggga ttggaacatt
     4201 cgcattggcg ctgccgggac cggtctacgc cgccgaagcc tcgcgcgatg cggtgctggc
     4261 gctcaagcgc gcctccccgg ccatcgcgac cgagcaccgc gacctctacc gccgtccgct
     4321 cgactcctcg gagctcgcaa acttcgaggc ggtcgtgctc gacccgccgc gggccggggc
     4381 cgccgagcag gtcgcggcgc tggcgtcgtc gagcgtgctg cggatcgctt atgtcagctg
     4441 caacccggcg accttcgcgc gcgacgcaag gacgctggtc gacggcggct acgcgctttc
     4501 ctgggtgcgt ccggtcggcc agttccgctg gtcgacccat gtcgagctgg ccgccgcctt
     4561 cgagcgctag atcaggccgt tatcgtgcag cgcccgggcg gcggcgtgga tcgtcatcgc
     4621 gatcaggcac agcgaagcga ggaagaacag cggcgtccgg tagacgagtc ggttgacggt
     4681 cgcctggacg atgctgtgga gcacccggaa gacgacatag ccccacgcca gccacaggtt
     4741 gaggccgccg cccatgtcca tcagcgcgag cgtgacggcg atcgcgtaga acagggtcgg
     4801 ctgctccatc agatggttgt aattgtgcga cttccactgg acccggtcgt cgaccacccc
     4861 gtcgagctgg gcgcccctcg ctccgggcgg cgcgctcgac agcgagatgc cggccttgtt
     4921 catcgccgga agacgaaccg cgaccatcca cagcatcatc accagcgacc aggcgaccag
     4981 cgccaccacc ggcgccagga tcggactata gctcattgct catccccctc gttcaggggg
     5041 cgactgtgcg gcagaatgcg gagctgcgca atcagtcgaa cagtttggcg aagcgccgct
     5101 tggcgcggtt gcgccaagcg ccgcggtgcc aggcgtcgga cgccagcagc ggcagaacgc
     5161 tggtggcttc cgcgaacacc atctgctcct gcgcggtgtc gaccttgccc cagctgcacg
     5221 cttcctgcag cgtcgaggac gagcaggcgc cgtcgcggac gtcggcgacc gtgatctgga
     5281 ccgcatattt gtgcatctcg acgtcctcgt ggccgaggat ttccgcgcac accaccgtgt
     5341 cctgggcgaa attcttcggg accccgccgc cgatcatcag caggccggtg gtgccggcct
     5401 tgatcttgat gtcggtcagc tcgcggaagt cggcgatgct gtcgagggtc atgtaatggc
     5461 cgccgcgctt catcgcgtcg gcctggtgct tgaccaggcc gaagccggcc gacgagtcgg
     5521 tgaacgccgg gcagaagatc ggcacgtcat gctcgaacgc gagcttgacc aggctgttgt
     5581 ccttcttgcc gttcttcgtg agccacttgc ccatctcgcg gatgaactcg cgcgacgagt
     5641 aaggccgcgg ctcgagcccg tccgcgatct tgccgatggt gaagtcggtc tgctgcagcg
     5701 cctcctcgtc gatataagtg tcgtagatgc ggtcgatgta cagcgagcgc agcgtcttat
     5761 cgtcagggat ttcaagggcc tggtagtgct tgtggccaag cgcttcgaag aagtccatgt
     5821 cgacgatggc cgccccggtg gcgacgatcg cgtcgaccat gttggagcgc accagctcgg
     5881 cgtagaggtc catgcagccg ccggccgagg tcgagccggc gatcaccagg aagatcgagc
     5941 agtcggggtc gtcgagcatc gcattgtaga tgcccgtggc gcgggcgagg tcgcggctgg
     6001 tgaagctcat cttgcccatc gcctcgacga tcggccgcgc atcgaagctg gtgatgtcga
     6061 tatgctcgac cgggctcgac agcagctcgg ccttgcgggt gtcgttgacc ttggcggtgt
     6121 cgatcaggga ttcggtggtc atgggattcc tcacaaaaag acgccgttcg ggctgagctt
     6181 gtcgaagccc tgcacttctc gaacaggaag tcactacagc ccttcgacaa gctcagggcg
     6241 aacggagcta aacgttccta aagcttcacg gccttggaac gcttgcgctc ctcgacggct
     6301 ccgtacagcg tcgccatcgg ctcgtcgtcg acgatctcga cgccctcgac cccgaagccg
     6361 ttgaagccgg tgcgcatcgc gcagccgtag gcgccgagca tgccgatctc gatatagtcg
     6421 ccggccgcga cgtcgccggg aagctcgaac ggccccgcca tgcggtcgag gtcgtcgcag
     6481 gtcggcccgt agaagctgaa ctccatcggc cgcaccttcg atgcggactc gcgcagcagc
     6541 cgcaccgggt agcgccagcc gatgtgcgcg gcgtcgaaca gcgcgccata agcgccgtcg
     6601 ttgatgtaca gctcggcatc gcggcgcttc tcgacgcgga caaggacgct ggcatattcg
     6661 gcgcacagcg cacggcccgg ctcgcaccac aattcggccg agtagctgat cggaagcgcc
     6721 tcgaagccgg cgtggatgac gttgaaatag gcctcgagcg ggggcggctc catgcccggg
     6781 taggtcgacg ggaagccgcc gccgacatcg accacgtcga ccgtcaccgc ggcctcgacg
     6841 atcgcgtcgc ggacgcggtc catcgcgtcg gaaaaagccg ccggggtcat cgcctggctg
     6901 ccgacgtgga agcagatgcc gagcgcgtcc gccgcctggc gggcggcgaa cagcagcgcc
     6961 ttgacgtcct gcggctcggc gccgaacttc gaagcgaggc tgagccgcgc gtgctccgag
     7021 ctgacctgca ggcgcaccag caggtacagg tcggtggccg cgacgccgtt ggtggcggtc
     7081 gagcgcagga tcttgtcgag ctcgtcgaac gagtcgaggc tgaaggcgcg gacattatgc
     7141 tcgaaatagg cctcggcgat cgcttcctcg gccttgaccg ggtgcatgaa gcagatgacc
     7201 gcttgcggca gcgtcctggc gaccaggcgc acctcgccga tcgaggcgac gtcgtaatgg
     7261 gtgacgccct cgtcccacag gatcttgagc aggtccggcg acgggttggc cttgaccgca
     7321 tagagcgacc ggcccggaaa cttctcgacg aagtagcggg cggcgcgccg ggcggcgtgc
     7381 gggcgcagga gcgtgaccgg ctggaccggg cgtcgtttcg ccaggtcggc agcgcaagcg
     7441 atgccgctgg gcttggcgag atcggcacct cggcgggtgc cggtcggggc ggtcgctagc
     7501 cccagcgcgc gatgatgctt gtgcaactca agggacctcc aacgggtacg ttcatccaag
     7561 ctgccttgcg gttggaagtc ccatggggca gcggggcgcg atatatggac ggggggtcgc
     7621 caagtaaagg tttttccggg tttcccgggc gcgtcagcat caccctgtcc ccctggcgcc
     7681 ac
//
LOCUS       DASTZO010000201         3156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_848891,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000201 DASTZO010000000
VERSION     DASTZO010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3156
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_848891"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..1157)
                     /locus_tag="VFU20_05570"
     CDS             complement(135..1157)
                     /locus_tag="VFU20_05570"
                     /inference="COORDINATES: protein motif:HMM:NF025034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HEU5285964.1"
                     /translation="MRAIASSKVLGALALAVSVAAVAAAQPATTRVDAVPGPPEIDGT
                     TVTEDIAFRNDRHDRMTVPVRVGGRGPYRFLVDTGADRTAVSTAVAAELGLSSGPMAT
                     LHSVTGMTPVRTANVPQLELTHNRVRSVEAPLLERVHMGADGILGVDSLRSQRVLFDF
                     RTRMISIVPSARRIEREDKDAIIVRGRLKRGHLIVTSARANGVPITVVLDTGSELTMG
                     NSALRARLEARRRLGEGERIEMISVTGQLLVGDAFKLKRVEIGDVVLKDLVILFSEAP
                     IFRSLHLEDRPAVLFGMNAMRAFDRVSIDFAKKQLRMVVPQQSSLDWAVMADRRRALG
                     MASAQH"
     gene            1308..1946
                     /locus_tag="VFU20_05575"
     CDS             1308..1946
                     /locus_tag="VFU20_05575"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5285965.1"
                     /translation="MEEKPVETTVYSLTDAPPAPSERRESERQLTLFRVGSLVIGDRR
                     ELCLIKNVSAGGMMIRAYCAIEPGTRVSVELKCGEAVAGSALWVEGETIGLAFDEPVD
                     VVELLATSMQGPRPRMPRIEVDCVASIREGATVHRVMVRDVSQGGIKVATEKALGVGA
                     DVMVTLAGLAPVAGVVRWSESGCYGVTFNRVLPLPLLVDWLHEQRERGRAAS"
     gene            complement(1950..2363)
                     /locus_tag="VFU20_05580"
     CDS             complement(1950..2363)
                     /locus_tag="VFU20_05580"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HEU5285966.1"
                     /translation="MDQSSISQNRRSRRLNVLLAASIEVSGATVPVKLRNLSTEGALI
                     EGDGLPVEGSEVVFRRNDLCVRSRVAWIYGRQAGVAFRRAIAQKDVLRNIPMPKYRAP
                     PDFRRPSLACRELTPEERRLVQSWAWTPWGDSFGE"
     gene            complement(2398..2988)
                     /locus_tag="VFU20_05585"
                     /pseudo
     CDS             complement(2398..2988)
                     /locus_tag="VFU20_05585"
                     /inference="COORDINATES: protein motif:HMM:NF017855.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF938 domain-containing protein"
BASE COUNT          496 a         1085 c         1083 g          492 t
ORIGIN      
        1 tgcgctcggg gctgtggatc gaccgcgctc gcggcaccgg ggtcgcttat ttcgtcaccg
       61 gcctggccga agacccgccg cgcggccgga gcgcctaccg ggccgccgag gaagcggcgt
      121 tccggcgcac gctcctaatg ctgcgctgaa gccatcccga gcgcccggcg gcgatccgcc
      181 atcaccgccc aatcgaggct gctctgctgc gggaccacca tccgcagctg ctttttcgca
      241 aagtcgatcg agacccggtc gaacgcccgc atcgcattca tcccgaacag caccgcgggc
      301 cggtcttcga ggtggaggct gcggaagatc ggggcctcgg agaacaggat gacgaggtcc
      361 ttgaggacga cgtcgccgat ctcgacccgt ttcagcttga aggcgtcccc aaccagcaac
      421 tggccggtca ccgatatcat ctcgatccgc tcgccctcgc ccagcctgcg ccgcgcctcg
      481 agcctggccc ggagcgccga attgcccatc gtcagctccg agccggtgtc gaggacgacc
      541 gtgatcggca ctccgttggc cctcgcgctg gtgacgatca gatggccgcg cttcagcctg
      601 ccgcgaacga taatcgcgtc cttgtcctcg cgctcgatcc gccgcgccga cgggacgatc
      661 gagatcatcc gggtcctgaa atcgaacagc acacgctgcg agcgcagcga atcgacgccg
      721 aggattccgt cggcgcccat gtgcacgcgc tcgagcagcg gcgcctcgac gcttcggacc
      781 cggttgtggg tcagctcgag ctgcggcacg ttggcggtcc gcaccggagt cattccggtg
      841 acgctgtgca gggtcgccat cggaccactc gacaggccga gctcggcggc gacggcggtc
      901 gagaccgcgg tccggtcggc gccagtgtcg accaggaatc ggtacggccc cctgccgccg
      961 acccgcaccg ggaccgtcat ccggtcgtgg cggtcgttcc ggaaggcaat gtcctcggtg
     1021 accgtcgttc cgtcgatctc cggcgggccg gggaccgcgt cgacccgggt cgtcgccggc
     1081 tgcgcggcgg ccacggccgc gacggacacc gcaagcgcca gggcgcccag gaccttcgag
     1141 ctcgcaattg cccgcatcgg catgtccctt cgccggcatg ctacgcctgc gaccgtcggc
     1201 gcgcaacgaa gtggtaacgc tggcgcgcta gcaatggtcg cgagtggggc cggtcgacgt
     1261 gtgtcggcac gggtttgggc agcgcgccga agcactgagg gtggaccttg gaagaaaagc
     1321 cggtcgaaac gacagtctat tccctcaccg acgctccgcc ggcgccttcc gaacgccgcg
     1381 aatccgagcg ccagctgacc ctgttccggg tcggctcgct ggtcatcggc gaccggcgcg
     1441 agctgtgcct gatcaagaac gtctccgccg gcggaatgat gatccgcgcc tattgcgcga
     1501 tcgagcccgg aacccgcgtc tcggtcgagc tcaaatgcgg cgaggcggtc gcgggctccg
     1561 ccctgtgggt ggagggcgag acgatcgggc tggccttcga cgagccggtc gacgtggtcg
     1621 agctgctcgc cacctcgatg cagggcccgc gcccgcggat gccgcgaatc gaggtcgatt
     1681 gcgtggcctc gatccgcgag ggcgcgaccg tccatcgagt gatggtgcgc gacgtgtcgc
     1741 agggcggcat caaggtggcg accgaaaagg cgcttggcgt cggcgccgac gtgatggtga
     1801 ctctcgccgg gttggcgccg gttgcagggg tcgtccgctg gtccgagtcg ggctgctacg
     1861 gagtgacctt caaccgggtg cttccgctgc cgctgctggt cgattggctg cacgagcagc
     1921 gggagcgcgg gcgcgccgcc agctaggaac tattctccga aagagtcgcc ccacggagtc
     1981 caggcccagc tttggaccaa tcgccgctct tcaggtgtca gctcccggca agccaaggag
     2041 gggcgacgaa aatccggcgg cgcgcgatac ttgggcatag gaatgttgcg gagcacatcc
     2101 ttctgagcaa tcgccctccg gaaggccacg cctgcctgcc ttccataaat ccaagcgaca
     2161 cggctgcgga cgcagaggtc gttgcggcgg aacactactt cggaaccttc aaccggcagg
     2221 ccgtcgcctt caatcagggc cccttccgtg gaaaggttgc gtagcttgac cggcactgtc
     2281 gcccccgaaa cctcgattga ggccgctagg agcacgttca agcgccgcga gcgtcgattc
     2341 tggctgatgc tgctctggtc catgatgcga ccataatgga ccttagtaaa actaggttta
     2401 acaggaacct ccgacctggc gcagcaacag catcatattg ttggcgggca ttggccgggt
     2461 ctcgaccagc gccaggccgc gctcgccggc ggcggaggcg aactcctcga cccggcgaag
     2521 gccccagtcc gggtcgcggc gcctgaggtc cgcgtcgaac tcgaggttgc tcggcgcggt
     2581 ggggatcgca tcggtcagcc acgggccgta gaggatcaac ggcccgcctt cgggcagcag
     2641 ccacgccgct ccgtcgagca ggccgagcgc cgccggccac gcgctgatgt gcaccatgtt
     2701 gatgttgagg acggcatcgg cccgctcgat cggccagtcc ggctcggcgg catcgatgac
     2761 gatggcgggc ttgagattgg gcaggcgcgc ttcggcccgc caggcggcaa tggaagcaag
     2821 cgcatcagga tggacgtcgc tcggctgcca gtcgagattc gggaaacgct cggcgaaata
     2881 gaccgcatgc tcgccggtgc cgctggcgat ttccagcacc agtccgcccg gcggcagcca
     2941 gtcacgcagc acctcggcga tcggctcgcg gttgcgcagc gccgccggcg ccgagcggcg
     3001 cgaagtatcg cccgccggcg cctcttagaa tcggcggtcg cccaccctat accgccgccc
     3061 ccgtatgctc cggcgccggc tccgggtcgc ggtaggcgac caccggccgg cgcgccgcca
     3121 gcgtctcgtc gagccggcgg cgcggcgcgt ggaacg
//
LOCUS       DASTZO010000202         5252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_65060,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000202 DASTZO010000000
VERSION     DASTZO010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65060"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..131
                     /locus_tag="VFU20_05590"
     CDS             <1..131
                     /locus_tag="VFU20_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEU5285967.1"
                     /translation="AEEVEHRDSAREAGAERAFGYPLLTAAVRAGCRLAIGLSKRV"
     gene            196..603
                     /locus_tag="VFU20_05595"
     CDS             196..603
                     /locus_tag="VFU20_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285968.1"
                     /translation="MTKLILQLAASAAFAAGLAASSAPAVAQDSDRISEIIVYGNDPC
                     PRSTDDDIVVCARKPESERYRIPERFRTGGPRQTREAWANKARALETVGATGINSCSP
                     VGPGGFTGCLVQVIKQARSESNEAVEQGTAPEE"
     gene            complement(613..2373)
                     /locus_tag="VFU20_05600"
     CDS             complement(613..2373)
                     /locus_tag="VFU20_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEU5285969.1"
                     /translation="MHYRSLALAAAAALALATPALAGPAEDFKALTDEYWAFVLRENP
                     TWASSLGHREYDALLPDISLAAEDRRAAQAERFLARLNAIPDSGLSTADRANKAILKR
                     GLEEWIEGNRFGQRMMLFTNREGWHQSIAGLSDSLTFRTRADYENYLKRLASYPAYND
                     EALAISTRALNEGYTLPCAAMGGFENTISGVIPADPAKSRLYAPFAAARPSTVSESEW
                     ASLQARAKALIGGDLRAAYAKHLAWYQTQYAPKCNQSVGVSAQPNGAAYYNYRIRQMT
                     TTGKSADEIHKLGLSEVKRIRAEMEEVARKAGFPNREAFIADLRTNPKYYAKTPEELM
                     QAVAREAKKIDGKMPTLFTLLPRLPYGIKEIPPELAPGTTTAYYNSGSPEQGIAGFYF
                     VNTSKLDQRPLWEVPALTVHEAVPGHHQQIALQQELPMPDWRRHGTFFTAFVEGWGLY
                     SERLGIEMGLYDTPAKDMGRLSYEMWRAARLVVDTGLHSKGWSKERAVRFMTDNTALS
                     AANIDAEVNRYISWPAQALAYKLGELKIRELRSRAEKQLGHKFDLRRFHDAVLGQGAV
                     PLDTLDAQINAWIAAEKVKA"
     gene            2527..3339
                     /locus_tag="VFU20_05605"
     CDS             2527..3339
                     /locus_tag="VFU20_05605"
                     /inference="COORDINATES: protein motif:HMM:NF018436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1206 domain-containing protein"
                     /protein_id="HEU5285970.1"
                     /translation="MSTVPDDLAGESRFQHWTRVGFAARGLLYIVIALLVLGTGRTED
                     LTGALEYLGRGVGRLLLMVLAAGLATYGLWRLADAAFGMENPGPDGKALRKRGAAGVI
                     GIIYLYLAYKAARILLDGDAGGSSTQQQADTVLDLPGGQWVLAFAALVLAVAGAYQLY
                     KAYKCSFLRRLDVAAQAPAVKWLGRIGYASRGVIFLAVALLIARAAIDEQSTEAGGME
                     QALDLLSGPWLYAVAAGLMLFGAFSIIEARFRRIHEPPVEEIKQEVRDGIGR"
     gene            3482..4087
                     /locus_tag="VFU20_05610"
                     /pseudo
     CDS             3482..4087
                     /locus_tag="VFU20_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008602930.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision.; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
     gene            complement(4084..>5252)
                     /locus_tag="VFU20_05615"
     CDS             complement(4084..>5252)
                     /locus_tag="VFU20_05615"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5285971.1"
                     /translation="NIYPNGAVDSARRVRISAYNDTTKRRNLIFQTDLVWDNRLGGVD
                     QTLLFGVELGSQKGRNQRRNGVFSSRTGSPTSILVPVDDPTIDEPVTFLANGANNGIK
                     ASIAAAYVQDQIRFTEWLELVAGLRFDRFSIDVENRNNGQRFRRTDKLWSPRLGLIVK
                     PRPNLSLYGSYSRSYLPSAGDQFNSLDLTSEALKPEKFDNYEVGAKWEPIAGLLATAA
                     IYQLDRTNTRATDPANPQRVVLTGAQRSRGLELGLERSVTHRWQISAGYALQKAKVTE
                     PTQACPPGDCEVPLVPRHSLSLWNRYDVTDKFGVGLGVIARSKSYASIGNAVKLPGYA
                     RVDTAAFYRLARGVEVQVNVENLLGADYFPTAHNDNNIAPGAPRTVKATLRFGL"
BASE COUNT          789 a         1718 c         1846 g          899 t
ORIGIN      
        1 gggccgagga ggtcgagcat cgcgacagcg cccgggaggc tggcgccgag cgcgctttcg
       61 gctatccgct gttgactgcg gcggtccgtg ccggatgccg gctggccatc ggcctgtcga
      121 agcgcgtttg atgattttga aggaacttct cttcgcactc gcccgcttta agggcttgaa
      181 aggatgtcat tcccaatgac gaaattgatc ctccagctcg ccgcgtccgc cgctttcgcc
      241 gccggcctcg ccgcgagttc cgcgcccgcg gtggcccagg acagcgaccg catcagcgag
      301 atcatcgtct acggcaacga cccctgcccg cgctcgaccg acgacgatat cgtggtttgc
      361 gcgcgcaagc cggagagcga gcgctaccgg atcccggaga ggttccgaac cggcgggccg
      421 cgccagaccc gcgaggcgtg ggccaacaag gcccgcgccc tggaaacggt cggagcgacc
      481 ggtatcaaca gctgctcgcc ggtcggcccg ggcggcttca ccggctgcct ggtccaggtc
      541 atcaagcagg cgcgcagcga gtccaacgaa gccgtcgaac agggcaccgc tccggaagaa
      601 taagccgggc ggctaggctt tcaccttttc cgccgcgatc caggcgttga tctgcgcgtc
      661 cagggtatcg agcggcaccg cgccctggcc gagcacggcg tcgtggaagc ggcgcaggtc
      721 gaacttgtgg ccgagctgtt tttccgcccg cgacctcagc tctcggatct tcaattcgcc
      781 gagcttgtag gcgagcgcct gcgccggcca gctgatgtag cggttgacct cggcatcgat
      841 gttggccgcg gacagcgccg tgttgtcggt catgaaccgg accgcccgct ccttcgacca
      901 gcccttcgag tgcagcccgg tgtcgaccac cagccgcgcc gcgcgccaca tctcgtagct
      961 cagccggccc atgtccttgg cgggggtgtc gtacaggccc atctcgatcc cgagccgctc
     1021 cgaatatagc ccccagccct cgacgaaggc ggtgaagaag gttccgtgcc ggcgccagtc
     1081 cggcatcggc agctcctgct gcaaggcgat ctgctggtgg tggccgggca ccgcctcgtg
     1141 cacggtcagc gccggcactt cccacagcgg ccgctggtcg agcttggacg tgttgacgaa
     1201 gtagaagccc gcgatgccct gctcgggcga gccgctgttg tagtaggccg tcgtcgtccc
     1261 cggagcgagc tcgggcggga tctccttgat cccgtagggc agccgcggaa gcaaagtgaa
     1321 cagggtcggc atcttgccgt cgatcttctt cgcctcgcgg gcgaccgcct gcatcagctc
     1381 ctcgggcgtc ttggcataat atttggggtt ggtgcgcagg tcggcgatga aggcctcgcg
     1441 gttcgggaag ccggctttcc tcgccacctc ctccatctcc gcccggatgc gcttgacctc
     1501 ggagaggccg agcttgtgga tctcgtcggc gctcttcccg gtcgtcgtca tctggcggat
     1561 ccggtaattg taataggccg cgccattggg ctgggccgac actccgaccg attggttgca
     1621 cttgggcgca tattgcgtct ggtaccaggc gaggtgcttc gcataggcgg cgcgaaggtc
     1681 gccgccgatc agcgccttgg cgcgcgcctg aagggatgcc cattcggact cgctgaccgt
     1741 cgacggccgg gcggctgcaa acggcgcgta gagccgcgac ttggccggat cggcgggaat
     1801 gaccccgctg atcgtgttct cgaacccgcc catcgccgcg cacggcaatg tgtagccctc
     1861 gttgagggcg cgcgtgctga tcgccagcgc ttcgtcgttg taggcgggat atgaagcgag
     1921 ccgcttcaaa taattctcgt aatccgcccg ggtgcggaac gtcaggctgt cgctcaggcc
     1981 ggcgatgctc tggtgccagc cctcgcggtt ggtgaacagc atcatccgct ggccgaagcg
     2041 gttgccctcg atccattcct cgagcccgcg cttgaggatc gccttgttgg cccggtcggc
     2101 ggtcgacagg ccggagtcgg ggatcgcgtt cagccgggcg aggaagcgct cggcctgcgc
     2161 ggcccggcgg tcctcggccg cgaggctgat gtcgggcagc agcgcgtcat attcgcggtg
     2221 gccgagggac gacgcccagg tcgggttctc gcgcaggacg aacgcccaat attcgtcggt
     2281 cagggccttg aagtcctcgg ccggcccggc gagcgcgggt gtggccaggg cgagcgcggc
     2341 agcggcggcg agtgcaagcg aacggtagtg catggattcc agtccctccc cgaaaacgcg
     2401 gacaggctta gccgcgcatc gcggctgcgc aaactcactt tcgaccggcg gatcaacggc
     2461 accgtttcgg ccccgcctgc gtttgggctg ccagcgcatc ttgccgggac gaaaggaaat
     2521 tccgccatga gcaccgtccc ggacgacctt gccggcgaga gccgcttcca gcactggacc
     2581 cgggtcgggt tcgccgctcg cgggcttctg tacatcgtca ttgcgctgct ggtgctcggc
     2641 accggccgga ccgaggatct gaccggtgcg ctcgaatatc tcgggcgcgg ggtcggccgc
     2701 ctgctgctga tggtgctggc cgcggggctg gcgacctatg ggctgtggcg gctcgccgac
     2761 gccgcgttcg gaatggaaaa tccgggcccc gacggcaagg ccctgcgcaa gcgcggcgcg
     2821 gcgggggtca tcgggatcat ctacctctac ctcgcctaca aggcggcgcg gatcctgctc
     2881 gacggcgacg ccggcgggtc cagcacccag cagcaggctg acaccgtgct cgacctgccc
     2941 ggcggccagt gggtgctcgc cttcgccgcc ctggtgctcg cggtggccgg cgcctaccaa
     3001 ctctacaaag cgtacaaatg ctcgttcctg aggcggctcg atgtcgccgc ccaagcgccg
     3061 gcggtcaaat ggctcgggcg gatcggctac gcatcgcgcg gagtgatctt cctcgccgtc
     3121 gcgctgctga tcgcccgagc ggcgatcgac gagcaatcga ccgaggcggg cggcatggag
     3181 caggcgctcg acctgctgtc cgggccttgg ctctacgcgg tcgcggcggg gctgatgctg
     3241 ttcggggcgt tcagcatcat cgaggcgcgc ttccgccgga tccacgagcc cccggtcgag
     3301 gagatcaagc aggaagtgcg cgacggcatc ggccgctgag acgcaacatc gccgagcctt
     3361 cgctcgtttc ctgccggtgg aggcggccat ggccggagat tcgagggaac tgtcgttcga
     3421 tttcgccgaa gagggtctcg agtcggcgct caaggccgag cggcgcatcg agccgggaag
     3481 caatctgcga tcgtcgtggg aagcgctctt gaaggaagcc cgcgcctgca cccgctgcga
     3541 gctctacaaa tgcgccaccc agacggtgtt cggcgaaggc ccgctcgacg cgtcgatcgt
     3601 cttcgtcggc gagcagccgg gcgaccagga ggatcttgcg ggacgtccgt tcgtcgggcc
     3661 ggccggcctg ctgttcgact cggcgctggc cgaggcgggc atcgaccgct cgaccaccta
     3721 cgtgaccaac gcggtcaagc atttcaaatt cgtggctcgc ggcaagcggc ggatccacaa
     3781 caagcccgac gccggcgaga tcgaggcgtg ccgctggtgg ctcgaccagg agcgggcgct
     3841 gatccggccg ccggtgacgg tcgcgctggg agcgaccgcc gcgcgctcgc tgaccggcaa
     3901 gatcgtgacc atcagccgcg cccgtgtggc gccgctgacc ctggccgacg gctcggaatg
     3961 ctgggtgacc gtgcacccga gcttcctgct gcgaatcccc gagcccgacc gccgccgcga
     4021 ggagcgcgcg aagttcgtga ccgacctcaa gcgcatcaag gcgcgggcgg ataagctggc
     4081 ggcttaaagc ccgaagcgaa gggtcgcctt taccgtgcgt ggggcgcccg gagcgatatt
     4141 gttgtcgttg tgcgcggtcg ggaaatagtc ggcgccgagc agattctcga cgttgacctg
     4201 cacctcgacg ccgcgtgcca gccggtagaa agcggcggtg tcgacgcgcg catagccggg
     4261 cagcttcacc gcattgccga tcgacgcgta ggacttcgag cgggcgatga ctcccagacc
     4321 gacaccgaac ttgtcggtca cgtcgtagcg gttccacagc gagaggctgt ggcgcggcac
     4381 cagcggcact tcgcagtctc ccggcgggca cgcctgggtc ggctccgtga ccttcgcctt
     4441 ttgcagcgca tagccggcgg aaatctgcca gcggtgggtg acgctgcgct cgagcccgag
     4501 ctcgaggccg cggctgcgct gcgcgccggt gaggaccacc cgctgcgggt tggcggggtc
     4561 ggtagcgcgg gtattggtgc ggtcgagctg gtagatggcg gcggtcgcca gaagcccggc
     4621 gatcggctcc cacttggcgc cgacttcgta attgtcgaac ttctccggct tcagggcctc
     4681 ggacgtcagg tcgagcgaat tgaactggtc gccggcgctc ggcagatagg agcggctgta
     4741 gctgccgtag agcgacaggt tcggccgcgg cttgacgatc aggccgaggc gcggcgacca
     4801 gagcttgtcg gtgcggcgga agcgctggcc gttgttgcgg ttctcgacgt cgatcgagaa
     4861 gcggtcgaag cgcagcccgg cgaccagctc gagccactcg gtgaagcgga tttgatcctg
     4921 gacgtaggcg gccgcgatcg acgccttgat cccattgttg gcgccgttgg cgaggaaggt
     4981 caccggctcg tcgatggtcg ggtcgtcgac cggcaccagg atcgaagtcg ggcttccggt
     5041 tcgcgaggag aagaccccgt tgcggcgctg gttgcggcct ttctggctgc cgagctcgac
     5101 tccgaacaga agggtctggt cgaccccgcc gaggcggttg tcccagacga gatcggtctg
     5161 gaagatgagg ttccgccgct tggtggtgtc gttgtaggcc gagatccgca ctcgccgcgc
     5221 gctgtcgacg gcgccgttcg gatagatgtt ct
//
LOCUS       DASTZO010000203         8110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_16950,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000203 DASTZO010000000
VERSION     DASTZO010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16950"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="VFU20_05620"
     CDS             complement(<1..305)
                     /locus_tag="VFU20_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285972.1"
                     /translation="MNEDKNPRSRRAGRWRRTSALCAVLSIAASAAVAKTFTGWSAPV
                     SAESLPGSSSQVNTASNDGCPILSPYDGSLYMASSRPGGHGGLDIWIAERSGDGWGAP
                     "
     gene            complement(439..939)
                     /locus_tag="VFU20_05625"
     CDS             complement(439..939)
                     /locus_tag="VFU20_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285973.1"
                     /translation="MFNFKRSMATRAILAIALCGTVTTMAAASPPTNVSPTNYVTEIM
                     EEPIKVHNDRIDLKARDASIVRMQLLTFGTNSTTGWHHHPGMVIVGVESGSIDLWDTN
                     CNRTTYGPGSPNGTVFIEALPHAHQATSTAGALVRVTYIVPSTGAVVPANFRVEEPVP
                     FCASSF"
     gene            complement(1057..2097)
                     /gene="nadA"
                     /locus_tag="VFU20_05630"
     CDS             complement(1057..2097)
                     /gene="nadA"
                     /locus_tag="VFU20_05630"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEU5285974.1"
                     /translation="MTAQPQSLRGLDLLAEIERLKRERNAVILAHYYQKPEIQDLADF
                     VGDSLDLSRKAAATDAEVIAFCGVRFMAETAKILSPEKTVILPDMDAGCSLEDSCPPD
                     QFKAFREAHPDHIALTYINCSAAVKALSDIIVTSSSADIILKQIPLDQKIIFGPDRHL
                     GGYLARKTGRDMLLWPGVCIVHQAFSETELLKLKAEHPGAPVLAHPECPPHIIDHADH
                     VGSTRSILEFALSSPAQTILVATEPHIIHQMEKAAPGKTFIGVPGGDGNCNCNMCPYM
                     ALNTLEKLYVALRDLKPRIELSPEIMDRARVPLERMLEMAGRTVGQGDVGRPQIDGHI
                     AAQDIDPGISGD"
     gene            complement(2094..2816)
                     /locus_tag="VFU20_05635"
     CDS             complement(2094..2816)
                     /locus_tag="VFU20_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HEU5285975.1"
                     /translation="MDARRLNRPMLVAGILAAMVLGLLIGVAAVADRMFGAPDPTTIA
                     TSSLESMRAQNRLIVFAARYVSVTSSRQSRLGFSAERTLILPGDVRYEVDLSKLRPED
                     VRWDSGSGTLSVRVPDIEIAGPQVDLASAREYGEGKLLTTIFGGEEQLDQANRAAAVA
                     DLRKQAMAELPMRLARGAARQAIERSFAMPLKAAGFDDAKVVARFASEENSADPSYID
                     QSISYNEAIEEARRRRAAEGTR"
     gene            complement(2798..3652)
                     /locus_tag="VFU20_05640"
     CDS             complement(2798..3652)
                     /locus_tag="VFU20_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5285976.1"
                     /translation="MDAPYAKLEPLEPGIARVLAANPSAFTYTGTQTYLVGEREVAVI
                     DPGPDLPEHLDALVEVIGGRPVAAILCTHTHRDHSPAARPLTQATGAPVIGCAPLALE
                     TVGPRADAAFDGDYRADRVLADGEGIAVDGRRLTAVATPGHTSNHLCFADEESGALFT
                     GDHVMGWSTTVVVPPDGDMAAYMASLDKLQQRDDRIYYPAHGPPVTNPRQHVRGFIGH
                     RKQRERQILALLAEHARTIPEIVAAAYPGLDPRLVTAAGGSVNAHLLDLEARGLVVRI
                     GETWTRAD"
     gene            complement(3699..4532)
                     /locus_tag="VFU20_05645"
     CDS             complement(3699..4532)
                     /locus_tag="VFU20_05645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285977.1"
                     /translation="MIAGHWLLGLAPLLAASGAIAQQPAPAGAPPARETSAAEAAAAQ
                     KIAACEGEKFEFTAGDQRPTRITLCSDKGASKDDLVRMFESAADKIRQIDKLPADRKA
                     ALVAQLEAKIIEVRSDNVLAIPLPAPRPVPEIGPKPEYAQLPPMPPPVRTPVVAQSSL
                     GPAVALSKPRLTLQCSTPGEIGEGGPCLNLAKDTVLTVRAGETLPAGTSLRFVRRGDV
                     RGEFALAQMRKGQARRIRLPLDVCSGVVSSEVEIQVLRRATATGLGQVVDTRGPYQLR
                     C"
     gene            complement(4600..5856)
                     /locus_tag="VFU20_05650"
     CDS             complement(4600..5856)
                     /locus_tag="VFU20_05650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5285978.1"
                     /translation="MSRDDPNLLGPLSAPRLFCDLTQSWSEVGGGVRTYLLHKRRHIL
                     DSTPHRHLMVIPGPRDEVIDEGRAVTVTIASPHVPGSPHYRLLLRNGAVCAALERHRP
                     DLIECQDAYNLPWAAIAHRNRHPGTALVAAYMTDFPTVYVERPFRKVIGNTLAGAASR
                     LCYRYCGHLYRRFDSVFALSENGGAAKLRAYGVPNVDIVGLGVELGDFGPSRRDPRLR
                     RKLGLGGEQPLLIYVGRLDGEKKPDVVADAFRRLPAGLGARLALIGEGPLREQIAAIG
                     DPRIITPGYLKDRAELAKWLASADIYVSGMADETFGVSIVEAQASGLPVVGVAAGAMI
                     DRVTERIGRLGPVNDSEAMAKNILAVWAGDRESMREAACKEARLYSWDRSMDALFGRV
                     YPDALARAVGRSLAPAATVAGSLAEA"
     gene            complement(6114..6428)
                     /gene="grxD"
                     /locus_tag="VFU20_05655"
     CDS             complement(6114..6428)
                     /gene="grxD"
                     /locus_tag="VFU20_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007686967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HEU5285979.1"
                     /translation="MSDAKERIAEIVGKNDVVLFMKGTPLFPQCGFSSRAVAILDHLG
                     VPFETVDVLQDPAIRQGIKDYSDWPTVPQLYVRGQFVGGSDIMMEMFESGELKQLVAQ
                     EA"
     gene            complement(6425..6670)
                     /locus_tag="VFU20_05660"
     CDS             complement(6425..6670)
                     /locus_tag="VFU20_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family transcriptional regulator"
                     /protein_id="HEU5285980.1"
                     /translation="MPMAAEEIERLIRDAIPDAQVTITDLAGDGDHYSARVVSATFAG
                     LDRVRQHQRVYAALGGRMGGELHALQLTTAAPSGDTK"
     gene            complement(6670..6999)
                     /locus_tag="VFU20_05665"
     CDS             complement(6670..6999)
                     /locus_tag="VFU20_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HEU5285981.1"
                     /translation="MTTFDDRERAFETKYAHDEELRFRVIARRNRLLGAWAARLMGLS
                     DAEADAYAKDVIRADFEEAGDEDVIRKLLGDLTAAGVDTDDSRIREALEHKEAEARRQ
                     IMEQLNL"
     gene            7370..7609
                     /locus_tag="VFU20_05670"
     CDS             7370..7609
                     /locus_tag="VFU20_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285982.1"
                     /translation="MLIFKQIGENDTQRVVRVPLTPQPYISSGTHHIKVPPQLCTQKG
                     ISRWQIQIATNDFNQPETSKVQDADSVGYFQMRCN"
     gene            complement(7606..>8110)
                     /locus_tag="VFU20_05675"
     CDS             complement(7606..>8110)
                     /locus_tag="VFU20_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5285983.1"
                     /translation="RVVAAVSSDEKAKAAREAGADESVVYPPGPFDREGQKALAELFK
                     SAVGPGGADVIFDPVGGDYAEPALRAIGWGGRYLVVGFPAGIPRLPLNLTLLKSCDVC
                     GVFWGAFAMRDPEANAAHVEHLFRLWEEGRIAPRISATFPLARAGEAIAAVAERRAIG
                     KLVVQVS"
BASE COUNT         1238 a         2794 c         2699 g         1379 t
ORIGIN      
        1 ggcgcgcccc agccgtcgcc gctgcgctcg gcgatccaga tgtccagccc cccatgtccg
       61 cccggccggc tcgacgccat gtagaggctg ccgtcgtagg gggacaggat cggacagccg
      121 tcattcgacg ccgtattcac ctgggagcta gaccccggga gcgattcggc gctcaccgga
      181 gcgctccatc cggtgaaggt cttggcgacc gctgccgatg cggcaatcga gagcaccgca
      241 cacaaagcac tcgtccggcg ccaacggcca gcacggcgcg accgcggatt tttgtcctcg
      301 ttcatgactc atcctctcca tttcgttcga agccatggca gcggattttc atgaggatgc
      361 gttcgctcga cgttatcgca gcgtttgcgc ggcgttaaat cgaccgctga cgcgccgaat
      421 ttggatggtt tgcgctccct aaaaggagga cgcgcaaaac ggcaccggtt cctcgacccg
      481 gaagttggcg ggcacgacag ccccggtgct cggcacgatg taggtcaccc ggacgagcgc
      541 gccggccgtg ctggtggcct ggtgcgcgtg gggaagggct tcgatgaaaa ccgtgccgtt
      601 gggcgagccc gggccgtagg tcgtcctatt gcaattcgtg tcccagagat cgatcgaacc
      661 cgattcgacc ccgacgatga ccatgcccgg atgatggtgc cagccagtcg tgctgttggt
      721 gccaaatgtg agcaactgca tcctgacgat cgaggcgtcc cttgccttga gatcgattcg
      781 gtcattgtga accttgatcg gctcttccat gatttcggtc acatagttgg tcggcgatac
      841 gttcgtcggc ggcgacgccg ccgccatcgt cgtgaccgtg ccgcaaaggg caatcgcaag
      901 aattgctctg gtagccatcg accgtttgaa attgaacatg cccaagccct ctcgtcgctg
      961 atttgttgag ggctgccgta tcggcgtcgg cgttcgctcg acgttatcgc agcgtttgcg
     1021 cggcgttaaa tcaaccggtg acgcgccgga atccgcttaa tcgccgctga ttcccgggtc
     1081 gatgtcctgc gcggcgatgt ggccgtcgat ctggggccgc ccgacgtcgc cctggccgac
     1141 ggtccgcccg gccatctcga gcatccgttc gagcggcact cgggcgcggt ccatgatctc
     1201 gggggaaagc tcgatcctcg gcttcaagtc ccgcagcgcg acgtacagct tctcgagcgt
     1261 gttcagcgcc atgtacgggc acatgttgca gttgcaattg ccgtcgccgc ccgggacccc
     1321 gatgaaggtc ttgccgggcg cggccttctc catttggtgg atgatgtgcg gctcggtcgc
     1381 gaccaaaatg gtctgcgccg ggctcgacag cgcgaactcg aggatcgagc gggtcgagcc
     1441 gacgtggtcg gcatggtcga tgatgtgcgg cgggcactcg gggtgcgcga gaaccggagc
     1501 gccgggatgc tcggccttca gctttaagag ctcggtctcg gaaaacgcct ggtggacgat
     1561 gcacacgccc ggccacagca gcatgtcgcg cccggtcttg cgcgccagat agccgcccag
     1621 gtgccggtcg ggtccgaaga tgatcttctg gtcgagcggg atctgcttca ggatgatgtc
     1681 ggcgctgctc gacgtgacga tgatgtccga cagcgccttc accgccgccg agcagttgat
     1741 gtaggtcagc gcgatatggt cgggatgggc ctcgcggaac gccttgaact ggtcgggcgg
     1801 gcacgaatcc tcgaggctgc agccggcgtc catgtcgggc aggatgacgg tcttttcggg
     1861 cgagaggatc ttcgccgtct cggccatgaa tcgaactccg caaaaggcga tcacctcggc
     1921 atcggtcgcc gccgccttgc gcgacagatc gaggctgtcg ccgacgaaat ccgcgaggtc
     1981 ctggatctcc ggcttctggt aataatgggc gaggatcacc gcgttgcgct cgcgcttcag
     2041 ccgctcgatc tcggcgagga gatcgagccc cctcaggctc tgcggctgtg cggtcatcgc
     2101 gttccttcgg cggcgcgccg tctgcgcgcc tcttcgatcg cctcattata tgagatcgac
     2161 tggtcgatat aggaagggtc ggcggagttc tcctcgctcg cgaaccgcgc gaccaccttg
     2221 gcatcgtcga agcccgcggc tttcagcggc atcgcgaagc tgcgctcgat cgcctgccgc
     2281 gccgctcctc gcgccaggcg catcggcagc tcggccatcg cctgcttgcg caggtcggcg
     2341 accgcggccg cgcggttggc ctggtcgagc tgctcctcgc cgccgaagat ggtcgtcagc
     2401 agcttgccct cgccatattc gcgggcgctg gcgagatcga cctgcggacc cgcgatctcg
     2461 atgtcgggga ctcggacgct tagcgttccg ctgccggagt cccaccgcac gtcctcgggc
     2521 cggagcttcg acaggtcgac ctcgtagcgc acgtcgccgg gcaggatcag cgtgcgctcg
     2581 gccgaaaagc cgagccggct ctgccgcgag ctggtcaccg acacgtagcg cgcggcaaag
     2641 acgatcagcc ggttctgggc gcgcatcgat tccaggctcg aggtggcgat ggtcgtcggg
     2701 tccggggcgc cgaacatgcg gtcggcgacc gcggcgacgc cgatcagcag gccgagcacc
     2761 atcgcggcga ggattcccgc gaccagcatc ggtcggttca gtcggcgcgc gtccatgtct
     2821 ctcctatccg aacaacgagc ccgcgcgcct cgaggtccag aagatgcgcg ttgaccgagc
     2881 cgccggccgc ggtcaccagc cgcgggtcga ggccggggta agcggcggcg acgatctcgg
     2941 ggatcgtccg cgcatgctcg gcgagcagcg ccaggatctg ccgctcgcgc tgctttctat
     3001 ggccgatgaa gccgcgcaca tgctgcctcg ggttggtgac cggcggcccg tgcgcggggt
     3061 aatagattcg gtcgtcgcgc tgctggagct tgtcgaggct cgccatatag gccgccatgt
     3121 cgccgtcggg cgggacgacg acggtggtcg accagcccat cacatggtcg ccggtgaaca
     3181 aggcccccga ctcctcgtcg gcgaagcaca gatggttcga cgtgtgccca ggggtcgcga
     3241 ccgcggtcag gcggcggccg tcgaccgcga tcccctcgcc gtccgcgagc acccggtcgg
     3301 cgcggtagtc gccgtcgaac gccgcgtcgg cccgcggccc gaccgtctcc agcgccagcg
     3361 gcgcgcagcc gatgaccggg gcgcccgtcg cttgcgtgag cggccgcgcc gccgggctgt
     3421 ggtcgcggtg ggtgtgggtg cacaggatcg ccgcgaccgg ccgcccgccg atcacttcga
     3481 ccagcgcgtc gagatgctcg ggcaagtcgg ggcccgggtc gatcaccgcg acctcgcgct
     3541 ccccgaccag ataggtctgg gtgccggtat aggtgaacgc cgacgggttg gccgcgagca
     3601 cgcgcgcgat ccccggctcg agcggctcaa gcttggcgta cggcgcgtcc acgagcgtgc
     3661 ctgtgccacg cgcgaccgat ggttggcaaa aagagcggtc agcagcgcag ctggtacggg
     3721 ccgcgcgtat cgaccacctg gcccagcccg gtcgcggtcg cgcgcctcag cacctggatc
     3781 tcgacctcgc tgctgacgac cccggaacag acgtccagcg ggagcctgat ccggcgcgct
     3841 tgtcctttgc gcatctgcgc cagggcgaat tcaccccgga catcgcccct gcggacgaag
     3901 cgcaggctgg tcccggccgg caaggtttcg ccggcgcgga cggtcagcac ggtgtccttg
     3961 gcgaggttga ggcacgggcc gccctcgccg atttcgcccg gtgtcgagca ttgcagcgtc
     4021 aggcgcggct tgctcagagc gaccgccggc ccgagcgacg attgcgcgac gacaggcgtc
     4081 cgcaccggcg gcggcatcgg cggcagctgc gcgtactcgg gtttcggacc gatctcgggc
     4141 accggcctgg gcgccggcaa cgggatggcc agaacattgt ccgaccggac ctcgatgatc
     4201 ttcgcctcga gctgggcgac cagcgccgcc ttccggtcgg ccggcaattt gtcgatctgc
     4261 cggatcttgt cggcggcgct ttcgaacatc cgaaccagat cgtccttgct ggcgcccttg
     4321 tcgctgcaca gggtgatcct ggtcgggcgt tggtcgccgg ccgtgaactc gaatttctcg
     4381 ccctcgcagg cggcgatctt ctgcgccgcc gcggcctcgg ccgccgacgt ctcccgggcc
     4441 gggggcgcac ccgcgggagc tggttgctgg gcgatggcgc cgctcgccgc cagaagcggc
     4501 gcgagcccga gcagccaatg tcctgcaatc atgattcgcc ctccccggaa cgggcatatg
     4561 tgttcgggcg cggcacgtga acccgccttt aaccgccggt caggcctcgg ccagcgatcc
     4621 cgccaccgtt gcggccggag cgaggctgcg gccgacggcg cgcgcgagcg cgtcgggata
     4681 gacccggccg aacagcgcat ccatgctgcg gtcccagctg tagagccggg cctccttgca
     4741 ggctgcctcg cgcatcgatt cgcggtcgcc ggcccagacc gctaggatgt tcttggccat
     4801 cgcctcgctg tcgttgaccg ggcccagccg cccgatgcgc tcggtgaccc ggtcgatcat
     4861 ggctccggcg gcgactccga ccaccggcag gcccgaggcc tgcgcctcga cgatcgacac
     4921 cccgaacgtc tcgtcggcca tccccgagac gtagatgtcg gcgctggcca gccatttggc
     4981 cagctcggcg cggtccttga gatagccggg cgtgatgatc cgcgggtcgc cgatggcggc
     5041 gatctgctcg cgcagcggcc cttcgccgat cagggccagc ctggcgccga gcccggcggg
     5101 cagccggcgg aacgcgtcgg ccaccacgtc cggctttttc tcgccgtcga ggcggccgac
     5161 gtagatcagc agcggctgct cgccgccgag ccccagcttg cggcgcaggc gcgggtcgcg
     5221 gcgcgacggg ccgaaatcgc cgagctcgac gccgaggccg acgatgtcga cattcgggac
     5281 gccgtaggcg cgcagcttgg ccgcgccgcc gttctcgctc agcgcgaaga ccgaatcgaa
     5341 acgccggtaa agatgcccgc aatagcgata gcagagccgc gacgccgctc cggcgagcgt
     5401 gttgccgatc accttgcgga acggccgctc gacatagacg gtcgggaaat cggtcatgta
     5461 ggccgcgacc agcgcggtcc ccgggtgccg attgcggtgc gcgatcgccg cccacggcag
     5521 attgtaggcg tcctggcact cgatcaggtc ggggcgatgg cgctcgagcg ccgcgcacac
     5581 cgcgccgttg cgcagcagca gccggtaatg ggggcttccg ggaacgtgcg gcgaggcgat
     5641 ggtcaccgtg accgcccggc cctcgtcgat cacctcgtcg cgcgggcccg ggatcaccat
     5701 caggtggcgg tgcggggtcg aatcgaggat gtgccgccgt ttgtggagga gataggtgcg
     5761 cactccgccg ccgacctcgg accagctctg ggtcaggtcg caaaacagcc tcggagcgct
     5821 caacggaccg agcaggttgg ggtcgtcgcg gctcatgcgc ctgtccaacc cgagacggcg
     5881 gacaaagtgc catccgcggc cgccggcgcg ccagactgtc ggctggtttt acactttgtc
     5941 acctgggcct gttttccatc gcgttggaaa tcccgaaaaa cagccgtttt ctgcgattgg
     6001 cacggccgtt gctatattaa tcccgtccac ttggttccac gccaatgaga gggacggtgc
     6061 agcggtcttt ctggtcccgc tccaccgtcc ctctttcatt gtggcttcgc gatttacgcc
     6121 tcctgcgcga ccagctgctt cagttcgccg ctctcgaaca tctccatcat gatgtccgac
     6181 ccgccgacga actggccccg gacgtagagc tggggcaccg tcggccagtc gctatagtcc
     6241 ttgatcccct ggcggatcgc cgggtcctgg agcacgtcga cggtctcgaa cgggaccccg
     6301 agatggtcga ggatcgccac cgcccggctc gaaaatccgc attgcgggaa caagggggtg
     6361 cccttcatga acagcaccac gtcgttcttg ccgacgattt cggcgatccg ctccttggca
     6421 tcgctcactt cgtgtctccc gatggcgccg cagtggtcag ctgcagcgca tgcagttccc
     6481 cgcccattcg cccgcccagc gccgcataga cgcgctggtg ctggcggacc cggtcgagcc
     6541 cggcgaaggt cgcgctgacc acccgcgcgg aataatggtc gccgtcgccg gcgaggtcgg
     6601 tgatcgtcac ctgagcgtcc gggatcgcgt cccggatcag ccgctcgatc tcttccgccg
     6661 ccatcggcat cacaggttca gctgttccat gatctggcgc cgggcctcgg cctccttgtg
     6721 ctcgagcgcc tcgcggatcc tggaatcgtc ggtgtcgacc cccgccgcgg tcaggtcgcc
     6781 gagcaatttg cggatcacgt cctcgtcgcc cgcttcctcg aagtcggcgc ggatcacgtc
     6841 cttggcatag gcgtcggcct cggcgtccga gagccccatc agccgcgccg cccacgcgcc
     6901 gagcagccgg ttacggcggg cgatgacccg gaaccgcagc tcctcgtcat gcgcatattt
     6961 ggtctcgaaa gcgcgctcgc ggtcgtcgaa agtcgtcatg gaatcctttc cctcgtctcg
     7021 cgcccgcaga taggagccgc cgcccagtcc gtccagcgct ccggcaagcg ccttcgattg
     7081 ccgctcttga gcgggaggag taatggaacg gccggaggtc gccatgtgcg gaattccatg
     7141 cgtcgcccgc gtgatgacgg ccgcagtgct cctgatcctg gctgcccctg gcctcgccca
     7201 gctctggagc cctctcgaca caaatcctcg ccccgccagg gccacgccta agcttagcct
     7261 gctctgcctg cccccgggcg gctcgggcgg aggcgttcct tgcagcaccg ggacgcgtga
     7321 gacagttctg atcgtgcgca ccgaaagggc aaccctcgat tcgcctcgct tgctgatctt
     7381 caagcaaatc ggggaaaacg acacgcagcg cgttgtgcgc gtcccgctca cgccgcaacc
     7441 gtacatcagc agcggaaccc accacatcaa ggtcccgccg caattgtgca cacaaaaagg
     7501 gatcagccgg tggcagatcc agattgccac gaacgacttc aatcaacccg aaaccagcaa
     7561 ggtgcaggac gcggattcgg tcggctactt ccaaatgcgc tgcaattagg agacttggac
     7621 caccagcttg ccgatcgccc ggcgctccgc gacggccgcg atcgcctcgc cggcgcgtgc
     7681 cagcggaaag gtcgcgctga tcctcggcgc gatccggccc tcctcccaca gccggaacag
     7741 atgctcgaca tgggcggcgt tggcttcggg gtcgcgcatc gcaaaggcgc cccagaacac
     7801 gccgcagacg tcgcagctct tgagcagcgt caggttgagg gggagcctcg ggatgccggc
     7861 cgggaagccg acgacgaggt agcgcccgcc ccagccgatc gcgcgcagcg cgggctcggc
     7921 ataatcgccc ccgaccggat cgaagatcac gtccgcgccg cccggtccga ccgcactctt
     7981 gaacagctcg gccagcgcct tctgcccctc gcggtcgaac ggccctggag gatagaccac
     8041 ggactcatcc gcccccgctt cgcgagcggc cttcgctttc tcgtccgacg acaccgccgc
     8101 cacgacccgg
//
LOCUS       DASTZO010000204         3000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_748122,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000204 DASTZO010000000
VERSION     DASTZO010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748122"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /locus_tag="VFU20_05680"
     CDS             <1..503
                     /locus_tag="VFU20_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEU5285984.1"
                     /translation="GSSYRGTCRGLPDDPARRAASPHSWRLDCGKALEIAGLPAWTED
                     KRRFTARPGDIGDAVLARKDAPASYHLACVVDDSASGVNLVVRGADLRPSTPVQRLLQ
                     ELLDLPEPAYLHHPLVTHADGRRLAKRDLAPTLAAIREAGADGPALAAELLAGKLPLG
                     FAFSGA"
     gene            514..720
                     /locus_tag="VFU20_05685"
     CDS             514..720
                     /locus_tag="VFU20_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIG1 domain-containing protein"
                     /protein_id="HEU5285985.1"
                     /translation="MSTVMIILLVAAMGATAYVLVRGVIAMASGKDVTGEQQQAWMRK
                     RVLFQAVAIVIVALILILAGASTG"
     gene            730..1299
                     /locus_tag="VFU20_05690"
     CDS             730..1299
                     /locus_tag="VFU20_05690"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU5285986.1"
                     /translation="MVRLNRIYTRTGDAGETGLVDGSRIAKDHPRAQAIGDVDELNSA
                     IGLAIVHVGSGKARAMLGCIQNELFDLGADLATPGDEFAPSETALRIVQGQVDRLETE
                     IDAMNEELEPLKSFILPGGGKGAAHVHLARAIARRAERSVITAARQVSLNPMARVYLN
                     RLSDHLFVLGRWLARDEGGDILWRPGATR"
     gene            1296..2516
                     /gene="egtB"
                     /locus_tag="VFU20_05695"
     CDS             1296..2516
                     /gene="egtB"
                     /locus_tag="VFU20_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0052699 - ergothioneine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ergothioneine biosynthesis protein EgtB"
                     /protein_id="HEU5285987.1"
                     /translation="MTARVGSLGERLAATRRLTLDLAGPLSDADATVQPFPDASPAKW
                     HLAHTTWFFETFVLRDHAPGYRRFDERYHYLFNSYYEGEGERHPRPRRGMLSRPSLDE
                     VREWRAHVDEALERALPGLPAGALDLVELGIHHEQQHQELLLTDILATFAENPLDPAY
                     GELEAATCHSPEPLSWLSGRTGIVEIGADEGGFAFDCERPRHPELLHPHAIASRPVTN
                     GEWAEFIADGGYSTATLWLSDGWDWVQREAITAPLYWRGDGTQFTLAGRRSIDPAAPV
                     AHVSLFEADAFARWAGARLPTEAEWESFAATADPNLGNQLDRAGAVVPAPPGDLFGDV
                     WEWTQSSFAPYPGFAPAPGAVGEYNAKFMSGQLVLKGASCATPRGHSRASYRNFFPPG
                     ARWQFTGLRLARDG"
     gene            2521..>3000
                     /locus_tag="VFU20_05700"
     CDS             2521..>3000
                     /locus_tag="VFU20_05700"
                     /EC_number="2.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HEU5285988.1"
                     /translation="MADGKAGDDQLGAFRDDVLAGLAAPIPAVPARWLYDHTGSELFD
                     QITRLPSYYPTRTETALLQAIMPEVAARIPEGAAVVEFGAGSAAKTPMLLRAIAPAAY
                     IPVDISGDYLKESAAHLSGRFPALPVLPVVADFVRPFDLPAAIAGLPKLGFFPGSTIG
                     "
BASE COUNT          410 a         1100 c         1052 g          438 t
ORIGIN      
        1 ccggctcctc ctatcgcggc acctgccgcg gcctgcccga cgaccccgcg cggcgggcgg
       61 cgagcccgca cagctggcgg ctcgattgcg gcaaagcgct cgagatcgcc gggctgccgg
      121 cctggacgga ggataagcgg cgcttcaccg cccggcccgg cgacatcggc gacgcggtcc
      181 tcgcccgcaa ggacgccccg gcgagctacc atctggcctg cgtggtcgac gattcggcga
      241 gcggggtgaa cctggtcgtg cgcggcgccg acctccgtcc ctcgaccccg gtccagcggc
      301 tgcttcagga gctgctcgat ctgcccgagc cggcctatct ccaccatccg ctggtcaccc
      361 acgccgacgg ccggcggctc gccaagcgcg atctcgctcc gaccctggcg gcgattcgcg
      421 aggcgggcgc ggacggtccg gcgctcgccg cggagctgct ggccggcaag ctgccccttg
      481 gtttcgcatt ctccggggcg tagaggccgc gccatgtcga cggtgatgat catcctgctg
      541 gtcgcggcga tgggcgcgac cgcctacgtg ctggtccgcg gggtgatcgc catggcgtcg
      601 ggcaaggacg tcaccggcga gcagcagcag gcctggatgc gcaagcgggt gctgttccag
      661 gcggtggcga tcgtcattgt cgcgctgatc ctgatcctcg ccggcgcctc gaccggctag
      721 gcggccgtca tggtccgact caaccggatc tacacgcgca ccggcgacgc cggcgaaacc
      781 gggctggtcg acggctcgcg gatcgccaag gaccatccgc gcgcgcaggc gatcggcgac
      841 gtggacgaat tgaacagcgc gatcggcctc gccatcgtcc acgtcgggtc cggcaaggcg
      901 cgggcgatgc tcggctgcat ccagaacgaa ttgttcgacc tcggcgccga cctcgccact
      961 ccgggcgacg aattcgcccc gtcggagacg gcgctgcgga tcgtccaggg ccaggtcgac
     1021 cggctcgaga ccgagatcga cgcgatgaac gaggaactgg agccgctcaa gagcttcatc
     1081 ctgccgggcg gcggcaaggg ggccgcgcac gttcaccttg cccgggccat cgcccgccgc
     1141 gccgagcggt cggtgatcac ggcggcccgg caggtgtcgc tcaacccgat ggcgcgggtc
     1201 tatctgaacc gcctgtccga ccatctgttc gtgctcgggc gatggctggc gagggacgaa
     1261 ggcggcgaca tcttgtggcg acccggcgcc acccgctgac cgcgcgcgtc ggctcgctcg
     1321 gcgagcggct cgcggcgacg cgcaggctga cgctcgacct cgccgggccc ttgtccgacg
     1381 ccgacgcgac cgtccagccg ttccccgacg cgtcgccggc caagtggcac ctcgcccaca
     1441 cgacctggtt cttcgagacg ttcgtgctgc gcgaccatgc gccaggctac cggcggttcg
     1501 acgagcgcta ccattatctg ttcaacagct attacgaagg cgagggcgag cgccacccgc
     1561 ggcccaggcg cgggatgctg agccggccgt cgctcgacga ggtccgcgag tggcgggctc
     1621 atgtcgacga ggcgctcgag cgggcgctgc cggggctgcc ggccggcgcg ctcgacctgg
     1681 tcgagctggg catccaccac gagcagcagc accaggaatt gctgctgacc gacatcctgg
     1741 cgaccttcgc cgagaacccg ctcgatccgg cctatggcga gttggaagcg gccacatgcc
     1801 attcgcccga gccgctgagc tggctttcgg gccgcaccgg catcgtcgag atcggcgcgg
     1861 acgagggcgg cttcgccttc gattgcgagc ggccgcggca cccggagctg cttcacccgc
     1921 acgcgatcgc cagccggccg gtgaccaatg gcgaatgggc cgagttcatc gccgatggcg
     1981 gctattccac tgcgaccctg tggctgtcgg acggctggga ctgggtgcag cgcgaggcaa
     2041 ttacggcgcc cctttactgg cgcggcgacg ggacccagtt cacgctcgcc gggcggcgct
     2101 ccatcgaccc ggcggcgccg gtcgcccacg tcagcctgtt cgaggccgac gcgttcgcgc
     2161 gctgggcggg cgcgcggctg ccgaccgagg ccgagtggga gagcttcgcc gccacggccg
     2221 accccaacct cggcaaccag ctcgaccgcg ccggagcggt cgtcccggcg ccgcccggcg
     2281 acctgttcgg cgacgtctgg gaatggacgc aaagcagctt cgcgccctac ccgggcttcg
     2341 ctccggcgcc gggcgcggtc ggcgaatata acgccaagtt catgagcggg cagctggtcc
     2401 tcaagggcgc cagctgcgcg actccgcggg gccacagccg ggcgtcctac cgcaacttct
     2461 tcccgcccgg cgcgcgctgg cagttcaccg ggctgcgcct cgcccgcgat ggctgatgcg
     2521 gtggcggatg gaaaggccgg cgacgaccaa ctcggcgcgt ttcgcgacga cgtgcttgcc
     2581 ggcctcgcgg cgccgatccc cgccgtcccg gcgcgctggc tgtacgacca cacaggctcc
     2641 gagctgttcg accagatcac ccggctgccg tcctattacc cgacgcggac cgagaccgcg
     2701 ctgttgcagg cgatcatgcc cgaggttgcg gcgcggattc ccgaaggcgc ggcggtggtc
     2761 gagttcggcg ccggatcggc ggccaagacg cccatgctgc tccgggcaat cgcaccggcc
     2821 gcctacatcc cggtcgacat ctcgggcgac tatctcaagg aaagcgcggc gcatttgagc
     2881 ggccgcttcc cggcgctgcc ggtgctcccg gtggtcgccg acttcgtccg cccgttcgat
     2941 cttccggccg cgatcgccgg cctgcccaag ctcggcttct tccccggctc gacgatcggc
//
LOCUS       DASTZO010000205         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_304068,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000205 DASTZO010000000
VERSION     DASTZO010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_304068"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /locus_tag="VFU20_05705"
     CDS             <1..562
                     /locus_tag="VFU20_05705"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5285989.1"
                     /translation="ALYCSRADRANEARTAIRRALVLDPLNARTHRAAGSIEYAARRY
                     RNALAPLRQALKLNPHMTFAHALIGYCLLQLGQLAQAREEFAAEPQATFRLSGLAIVD
                     RRLGNPEAAEKSLAQLVAEVGDSALYQQAQVHAQWGDQSAALTALERARVVGDSGLTY
                     LSTDPLLDPVRRQPRFLALLNDLGLR"
     gene            628..801
                     /locus_tag="VFU20_05710"
     CDS             628..801
                     /locus_tag="VFU20_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285990.1"
                     /translation="MIIGGKPVTDASEEALEQDKPNVAQARASGDPRASGDDRNSGDD
                     RNSGDDRNSGDDR"
     gene            complement(798..1658)
                     /locus_tag="VFU20_05715"
     CDS             complement(798..1658)
                     /locus_tag="VFU20_05715"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEU5285991.1"
                     /translation="MRITSWIIAASLAFHAGAAAAHDYNSGKRPALTFGAPWQAEIYS
                     NFAGYSEKERAEKELWELAHRCGGSLIAPQWVLTAAHCINQRQVDDGFRVRLGSRDLK
                     DRPGATYRIARIVRHAAYDKDKKLNDIALIHIVADDKTELGKAGRIRAIRLNGTSDYD
                     AAIEPGVSVSATGWGKTTPGKGGRASPQLLQVELETVDCDSAPDYRGRTTEDMLCAGA
                     PGKDSCQGDSGGPLILTHGEPVLVGIVSWGDDCGEAAHPGVYVRIDRQHYLDWIGRAM
                     AADPSVNSLD"
     gene            complement(1709..>2200)
                     /locus_tag="VFU20_05720"
     CDS             complement(1709..>2200)
                     /locus_tag="VFU20_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285992.1"
                     /translation="CPGGVIAEERARELARAVAAFEIEPQVAYHQKSARQIDALDHRL
                     ERVANWLFKATLVVSAAIVGGHLYAPDWVAEYSDWFTLVSAGFPALGTAVFGIRFQGD
                     FGGNAVRSQATANELRDIQLEMERGVELLRAADLAEQAARAMLEHLDEWRLVNQQMDL
                     GIA"
BASE COUNT          348 a          759 c          725 g          368 t
ORIGIN      
        1 cgcgctctac tgctcgcgcg cggatcgcgc caatgaggcg cggacggcga tccggcgggc
       61 cctggtcctg gacccgctca acgcacgaac ccaccgcgcc gcgggctcga tcgaatatgc
      121 cgcgcggcgc tatcgcaatg cgctcgcccc gctccggcag gcgctgaagc tcaatccgca
      181 catgacgttc gcccacgctc tgatcggcta ttgcctgctc cagctcgggc agctggcgca
      241 ggcgcgcgag gaattcgccg ccgagccgca agccacgttc aggttgtcgg gcctggcgat
      301 cgtcgaccgc aggctcggca accccgaagc ggccgagaag tcgctcgcgc agctggtcgc
      361 ggaagtcggc gacagcgctt tgtaccagca ggcccaggtc catgcgcaat ggggggatca
      421 gtcggccgcg ctgacggcgc tcgagcgcgc gcgagtcgtc ggcgactcgg gattgaccta
      481 tctttcgacc gacccgttgc tcgatccggt ccgccggcag ccaagattcc tggcgcttct
      541 caatgactta ggcttgagat gaaccgccgc cctatcaccg ggagcgcatc cgtgcgacca
      601 tgcgcgctcc tgccagggga ggaagcaatg atcatcggtg gaaaacccgt aacggacgcg
      661 tccgaagagg cgctcgaaca agacaagccg aatgtcgctc aggcccgcgc cagcggcgat
      721 ccccgcgcca gcggcgacga ccgcaacagc ggcgatgacc gcaacagcgg cgacgaccgc
      781 aacagcggcg acgaccgcta gtcgaggctg ttgaccgacg ggtcggcggc catcgcgcgg
      841 ccgatccagt cgagataatg ctggcgatcg atacggacat agacgccggg atgagcggct
      901 tcgccgcaat cgtcgcccca gctgacgatt ccgacgagca ccggctcgcc gtgggtcagg
      961 atcaacggtc cgccgctgtc gccctggcaa ctgtccttgc cgggcgcgcc ggcgcacagc
     1021 atgtcctcgg tcgtccggcc gcgatagtcg ggcgcggagt cgcagtcgac cgtctcgagc
     1081 tcgacctgca atagctgcgg cgaggcgcgg cccccttttc cgggcgtggt ctttccccat
     1141 cccgtcgcgc tgaccgaaac cccgggctca atggccgcat cgtagtcgct ggtgccgttg
     1201 agccggatcg cccggatccg cccggccttg ccgagctcgg tcttgtcgtc ggcgacgatg
     1261 tggatcagcg cgatgtcgtt gagcttcttg tccttgtcat aggcggcatg gcgcacgatc
     1321 cgggcgatcc ggtacgtcgc gcccggccga tccttgagat cgcgcgaccc gagccggacc
     1381 cggaatccgt cgtcgacctg cctctggttg atgcagtgcg cggcggtcaa aacccattgc
     1441 ggcgcgatca gcgacccgcc gcagcgatgc gcgagttccc acagctcctt ttccgcccgt
     1501 tccttttccg aatagccggc gaagttggaa tagatctccg cctgccaggg cgctccgaag
     1561 gtcagcgcgg ggcgcttgcc gctgttatag tcgtgcgcgg ccgcggcgcc ggcgtgaaat
     1621 gcgagcgagg cggcgatgat ccagctggtg atgcgcattc ggcttcccct tgggttccat
     1681 atatttacgg acgcgggtgg gtcggagtct aggcgattcc gaggtccatc tgctggttga
     1741 ccagccgcca ttcatcgaga tgctcgagca tcgctcgagc cgcttgttcg gctaggtcgg
     1801 cggcgcgcag cagctcgact ccgcgctcca tctccagctg gatgtccctg agctcgttgg
     1861 cggtcgcctg cgagcgcacc gcattgcccc cgaaatcgcc ctggaagcgg atgccgaaca
     1921 ccgccgtgcc gagcgccggg aagcccgctg acaccagggt gaaccagtcg ctgtactccg
     1981 cgacccagtc gggcgcgtac aggtgcccgc cgacgatcgc ggcggagacg accaaagtcg
     2041 ccttgaacag ccaattggcg acccgctcga gccggtggtc gagcgcgtcg atctggcgcg
     2101 cactcttttg gtgataggcg acctgcggct cgatttcgaa tgccgcgacc gctcttgcca
     2161 gttcgcgcgc ccgctcttcc gcgatgacgc cgccggggca
//
LOCUS       DASTZO010000206         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_957328,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000206 DASTZO010000000
VERSION     DASTZO010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_957328"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VFU20_05725"
     CDS             complement(<1..974)
                     /locus_tag="VFU20_05725"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEU5285993.1"
                     /translation="MTMLRTLPVAAALLATTAPAYAETETADAAATAEAGDVIVVTGQ
                     RREYGVRSTITATRTNTDVKDIPQALTVVSESQIEDQSLRSIADLLMFVPGATPGTGE
                     GNRDQITLRGNTTTADFFVDGIRDDVQYFRDFYNLDRVEVLRGSNAMIFGRGGGGGIV
                     NRVTKRSGLAQYREFLAAGDSEGGVRLSGDLDQPLGSSAGLRINGVYENGDSFRRHVD
                     LERLAVNPTVGILAGPATRIDLSYEYLRDRRTADRGVPSIAGEPLEGFDRTFFGDPGS
                     SFARADVHLATFGLEHKFGDALTLRNRTLYGDYDKFYQNIYPNGAVDSA"
     gene            complement(1036..2385)
                     /locus_tag="VFU20_05730"
     CDS             complement(1036..2385)
                     /locus_tag="VFU20_05730"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEU5285994.1"
                     /translation="MTGRLVPPYPPRRPGPVAVWRGLIGERARTLVYGWSERAFREPY
                     MRRNVLGYRVHIPLDPESVQRVLLDNAANYQKPRLVKRLLAPVIGRGLLSSDGTLWRE
                     QRRIVAASFTPPAVDALVPVFAKVAEAAAAGWGEGVRDVAREATATTMRVISNALFSG
                     DRRLTSAEAMAHIAAALDGVSEARMQVLLGLPMIPVSLRGYRGRRGQVYLRKTLSALV
                     RERLAGDAPDDFVTHMIHALRERFPADEAIELAVDNAATFYLAGHETTANTVTWTLYL
                     LAEQPELQEKAAAEAREALAAGVDSALPDRLPLLRMIVEESLRLYPPAPRFDREAVAA
                     DRLGEHDIRPGDLVSIWPWLLHRHQRLWDDPDTFDHSRFAPEARKGRHRFQYIPFGGG
                     PRMCVGARFAMAEGLAILAVWLARYRFAPVPGREVRLSGAVTLRPAGGMPLTLWRRS"
     gene            complement(2397..>3141)
                     /locus_tag="VFU20_05735"
     CDS             complement(2397..>3141)
                     /locus_tag="VFU20_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chromosome segregation protein"
                     /protein_id="HEU5285995.1"
                     /translation="ERFECVPQRLPEKLGFDAQEVLDPEAESAQLERLTADRERLGPV
                     NLVAEQELAEIDAARTSGAAEAEELTQAIHRLRGSIGSLNREGRVRLLAAYEKVDAHF
                     RSLFRTLFDGGQAHLELVESDDPLEAGLEIMAQPPGKRLSTLTLLSGGEQALTAIALI
                     FALFLTNPAPICVLDEVDAPLDDANVERFCDLLERMTQETDTRYLIVTHNAVTMSRMH
                     RLYGVTMIEKGISRLVSVDLSAATLLAAE"
BASE COUNT          458 a         1040 c         1166 g          477 t
ORIGIN      
        1 gcgctgtcga cggcgccgtt cggatagatg ttctggtaga atttgtcata gtcgccgtag
       61 agtgtgcggt tgcgcagcgt cagggcgtcg ccgaacttgt gctcgaggcc gaaggtggcg
      121 aggtggacgt cggcccgggc gaagctcgag cccgggtcgc cgaagaaggt acggtcgaag
      181 ccctcgagcg gctcgccggc gatcgacgga acgccgcggt cggcggtgcg gcggtcgcgc
      241 agatattcgt aggacaggtc gatgcgggtc gctggcccgg cgaggatgcc taccgtcggg
      301 ttgaccgcca gccgctcgag gtcgacgtgg cggcggaacg agtcgccgtt ctcgtagacg
      361 ccgttgatgc gcaacccggc gctgctgccg agcggttggt cgaggtcgcc gctgaggcgg
      421 acgccgccct cgctgtcgcc ggcggctagg aactcgcggt actgggcgag gcccgagcgc
      481 ttggtcaccc ggttgacgat tccgccgccg ccgccgcggc cgaagatcat cgcgttggag
      541 ccgcgcagca cctcgacccg gtcgaggttg tagaagtcgc ggaaatattg gacgtcgtca
      601 cggatcccgt cgacgaagaa atcggcggtg gtggtgttgc cgcgcagcgt gatctggtcg
      661 cggttgccct ccccggtgcc cggagtcgct ccggggacga acatcaaaag gtcggcgatc
      721 gagcgcagcg actggtcctc gatctggctt tcggaaacca ccgtcagcgc ctgcgggatg
      781 tccttgacgt cggtgttggt gcgggtcgcg gtgatggtcg agcggacgcc atattcgcgg
      841 cgctggccgg tgacgacgat gacgtcgccg gcttcggccg tggccgcggc gtcggccgtc
      901 tcggtctcgg cgtaggctgg agcggtggtt gcgaggaggg cggcggcgac tggaagggtg
      961 cggagcatgg tcatgacgag cgccactatc gttattgata gtcattcgca atagcatttc
     1021 agcggctcga gatatttagg agcggcgcca gagcgtcagc ggcatccctc cggccgggcg
     1081 gagcgtgacc gcgcccgaca atcgcacctc gcggcccggc accggcgcga agcggtagcg
     1141 ggcgagccac acggcgagga tggccagccc ctcggccatg gcgaagcggg cgccgacgca
     1201 catccgcggc ccgccgccga acgggatata ttggaagcgg tgccggccct tccgggcctc
     1261 gggcgcgaag cggctgtggt cgaacgtgtc gggatcgtcc cacagccgct ggtggcggtg
     1321 gagcagccac ggccagatgg acacgaggtc gcccggccgg atgtcatgct cgccgagccg
     1381 gtcggcggcg accgcctcgc ggtcgaagcg cggcgccggc gggtagaggc gcagcgattc
     1441 ctcgacgatc atccgcagca agggcagccg gtcgggaagc gccgaatcga cgcccgcggc
     1501 caaggcctcg cgcgcctcgg cggccgcctt ctcctgcagc tccggctgct cggcgagcag
     1561 gtacagcgtc caggtcaccg tgttggcggt ggtctcgtgc ccggcaagat aaaaggtcgc
     1621 ggcgttgtcg accgcaagct cgatcgcctc gtcggccggg aagcgctcgc gaagcgcgtg
     1681 gatcatgtgg gtgacgaaat cgtcgggagc atcgccggcg aggcgctcgc ggaccagcgc
     1741 gctcagcgtc ttgcggagat agacctggcc gcggcggccg cggtagccgc gaaggctgac
     1801 cgggatcatc ggcagcccga gcagaacctg catccgcgcc tcgctgaccc cgtccagcgc
     1861 cgcggcgata tgggccatcg cctcggcgct ggtcagccgc ctgtcgccgc tgaacaaggc
     1921 gttggagatc acccgcatgg tcgtcgcggt ggcctcgcgg gccacgtcgc gaacgccctc
     1981 gccccagccg gccgccgccg cctcggcgac ctttgcgaac accggcacca gcgcgtcgac
     2041 cgccggcggg gtgaagctgg cggcgacgat ccgccgctgc tcgcgccaca aggttccgtc
     2101 ggagctcagc aggccgcgcc cgatgaccgg cgccagcagc cgcttgacca gccgcggctt
     2161 ctggtaattg gcggcgttgt cgagcagcac gcgctggacg gactcggggt cgagcgggat
     2221 gtgcacccgg tagccgagca cgttcctccg catgtacggc tcgcggaacg cccgctccga
     2281 ccagccgtag accaaagtcc gggcgcgctc tccgatcagg ccgcgccaca ccgccaccgg
     2341 gccggggcgg cggggcggat acggcgggac gagtcgcccg gtcatcgtgc gctcggtcac
     2401 tcggcggcga gcagggtcgc ggcgctcaga tcgaccgaca ccagccggct gatgcccttc
     2461 tcgatcatgg tcaccccgta gagccggtgc attcggctca tggtcaccgc attgtgggtg
     2521 acgatcagat agcgggtgtc ggtctcctgg gtcatgcgct cgagcaggtc gcagaagcgc
     2581 tcgacattgg cgtcgtccag cggcgcgtcg acctcgtcga gcacgcagat cggcgccgga
     2641 ttggtcagga acagagcgaa aatgagcgcg atcgcggtca gcgcctgctc gccgcccgac
     2701 agcagggtca gggtcgacag gcgcttgccc ggcggctgcg ccatgatctc cagcccggcc
     2761 tcgagcggat cgtcgctctc gaccagctcg aggtgcgcct ggccgccgtc gaacagggtc
     2821 cggaacaggc ttcggaaatg cgcgtcgacc ttctcgtagg cggcgagcag ccgcacccgg
     2881 ccctcgcggt tgaggctgcc gatcgagccg cgcagccggt ggatcgcctg ggtcagttcc
     2941 tcggcctcgg cggcgccgct ggtccgcgcc gcgtcgatct cggcgagctc ctgctcggcg
     3001 accagattga ccggccccaa gcgctcgcgg tcggcggtca ggcgctcgag ctgggccgat
     3061 tcggcctcgg ggtcgaggac ctcctgggcg tcgaagccga gcttttcggg caggcgctgg
     3121 gggacgcatt cgaagcgctc g
//
LOCUS       DASTZO010000207        11395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1009415,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000207 DASTZO010000000
VERSION     DASTZO010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11395
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009415"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="VFU20_05740"
     CDS             <1..802
                     /locus_tag="VFU20_05740"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEU5285996.1"
                     /translation="GLYLLVSRGVAPLAYRLAHPQALANALIGQNGFLTAALFIAGMR
                     ALERRPLLGGAILGLLVIKPQLAVLLPIALLAGREWRAIAGAAASSIALLLLALLTFG
                     METYRGFFDIVPVYAHAMATSRWPWNELASVFAFARHLGVPQQPALIIHAMVALGATA
                     LVWRAWSRRSELRVPVLAAAILLVPPYLFTYDALLLIVPMGWLVVRERGPVVVAVAWL
                     CCLLPVVTYIGFYPGPNPIPIAALLCLWALHAEESRQAGPAGSVHAPA"
     gene            789..2192
                     /locus_tag="VFU20_05745"
     CDS             789..2192
                     /locus_tag="VFU20_05745"
                     /inference="COORDINATES: protein motif:HMM:NF021436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HEU5285997.1"
                     /translation="MRPPEEGAEIGPRRRSPEWLFAILALVGGIVLIGLIPPVGGGNE
                     RYNFHRAASAASAHLLVGPAPLPGGIAEFLEASREKFREGRNPPYSYSRADFDRMASI
                     PLRADEPRVVRPNPIAVMHPISYLPQSAAIALGRLLGLSPLALFYSGRISGLLAGVAL
                     TFLAIRTIPDHRHALAAVALLPPVLFTRSTLDADQFTVGLAFLFLALVIREIAASGRI
                     ARRTLAVLAAAAFILAQCKSAYLLLPLLSLAIPASRFGSVRAKALACLLVCAPGIVAS
                     AAWMIALKLSYFDNVAQYRTWSGIVRPGDQLQFVISDPLAYLRIVLATVFATPLVPKA
                     ILEFFGVFGPPVTLPLPFYPVAGALLAGVTFSGRPIPAGPLSNWRTRGFALALAAATI
                     LVILALLYLQWTRYRAPVIDGFQGRYLYPLIPLFLLSIPAAGRAFLRIPARHWLVALG
                     LFSIAATWWKTWETYLA"
     gene            complement(2282..2464)
                     /locus_tag="VFU20_05750"
     CDS             complement(2282..2464)
                     /locus_tag="VFU20_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEU5285998.1"
                     /translation="MVEGLRALLRDGSGASAAEYAVILAIVGTGIILAAIFLGEAVTG
                     ALSDASGCIGSDGSNC"
     gene            complement(2691..4406)
                     /locus_tag="VFU20_05755"
     CDS             complement(2691..4406)
                     /locus_tag="VFU20_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5285999.1"
                     /translation="MSVSIRPVRPGYDHARYVAAAAILLLVLVTGSIAFSHERFNLYT
                     RYIFLTQDAWLAPFFAAACLFHARPASRGRLFTAQRERRSMWLLLALLLLICWVGRFL
                     VFGDFDATPDERMAAFDAAVFRGGALVWPIPPEWRSLANALNRNFMLPIGSAEAWISA
                     YLPVNAAFHAVVGKLMDEDLTAPLLVAAGAWCLWRISLRLWPDSVSARIATVAFYAGS
                     SQVLITGMTKFAMSAHLALNLCWLMLFLRGDRRSHALAMLVGFLATGVHQPLFHPLFA
                     APFLALLGGRRQWSLLGAYLFAYACIGAFWVAWPTLLSSMGTGAVVPIESSSGIGYLE
                     RLLATVKGVDLEGAWLTGANLIRFITWQHPLLAPVALFGAWAAWRSEPFVRALVVSFA
                     LPILIFWILLPWQGSGWGYRYLHPVLGNAFLLGGWGWHMLERSHKDLWRPLLWTSAAS
                     LLLLLPVHGWLAHHIIRPIAQADARIDAIAADVVIVDTQFHEVFVHNRHDLSNRPIRL
                     MSAHVQPRQIARLCRRRSIAFVDPPAPGEPSGRQRALESAAGRAGCAASTVRVPIADH
                     LLRGR"
     gene            4645..5499
                     /locus_tag="VFU20_05760"
     CDS             4645..5499
                     /locus_tag="VFU20_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286000.1"
                     /translation="MKINIRVAALAAVAAAAIAAPAIASHSWSTYHWQRTSAEISPPV
                     ITAIDQTKWGTYVVNAVGDWNGQDPDQTPYISYIESPGPTASGLNPKTCKAQSGKILV
                     CNARYGFTGWLGIAQIWLSGGHIVQGITKLNDSYFDTATYNKPEWRALVACQEIGHDY
                     GLGHQDEDFNNADVEQPAGSQSCMDYTNQPLGNETPNGHDYEQLAIMYSHSHSNFTAV
                     GPGRSNSGTGLNNASGGDSPSEWGRAVHFDAQGRPDYFMMDLGGGKRKLTHVFWTLEA
                     KAGGHHGH"
     gene            5659..6390
                     /locus_tag="VFU20_05765"
     CDS             5659..6390
                     /locus_tag="VFU20_05765"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU5286001.1"
                     /translation="MYRNDLTSRDKSGAMAAVLAIHAVLAFALLHMSGTIEIDDPQRA
                     LRVFDIDEIVPPPPPPPPPQQQAERPKPKEEEGGSSPNLKSEATPVVAPKPRIELPVK
                     PPIAVTETPREGAAPTQGAADVRGPGTGTGGSGTGTGSGSGAGSGGGGGIATRAYKLS
                     PSLTMRDYPPHLRRRFPRGAGVLVTFTVLTNGRVTDCKVYQSSGDAEVDNMTCALVTS
                     RFVYRPAYNRRGEPVVSQAGYRQGD"
     gene            6398..7099
                     /locus_tag="VFU20_05770"
     CDS             6398..7099
                     /locus_tag="VFU20_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU5286002.1"
                     /translation="MIEAEWWDHDSAEEMADAVAGDVGFIVESALDARDSALIALPGG
                     TTPKPIYPKIAGRQLAWKRVTIIPTDDRLAPLDSELSNIRAIAQEFLPTGARVFPIAT
                     TIDDHKLAGNAADARLQDLSWPPDLVWLGMGADGHTASIFAGPDLQDALDAPKARRAV
                     GVMPDPMPADAPVPRVTLTRAAILSARTLLITITGPEKRSLLEQAIADGHSSKLPIGR
                     VLAEAEQPIDIHWCP"
     gene            7113..8492
                     /locus_tag="VFU20_05775"
     CDS             7113..8492
                     /locus_tag="VFU20_05775"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HEU5286003.1"
                     /translation="MKALAALALALAPLTSLHAMAPDPYAPEGFEGVRHPAWSRKAVI
                     YQVNTRQFTPQGTLKAAALEIPRLKALGVDILWLMPIHPIGEKNRKGTLGSPYSVRDY
                     RAVNPELGSFADLKAFVDAAHRNGVKVILDWVANHTAWDHPWATRHPDYYERDWKGSF
                     RSTPWWDWSDIIDLDYRNAAMRREMAQAMAFWVREAGVDGFRADVAGYVPPDFWTDVR
                     RDLEAIKPVWMLGEFNHRDLHMNAFDASYGWAWAEALMKIARGEADTGALYGFYSENE
                     SAWPTGAQRMIFTSNHDENAWSGTEFERFGPALANAFALLFTSEGIPLVYNGQEAGND
                     RRLKFFERDPIVWRKHPHGALIREWIRFRDAHPALHNAPWGARMVHVKNSESQKIFSF
                     VRARDGDAVFVAQNYSAEPRTATLEDIPHPGRWDERGGPAATLQKGARLTLAPWSTRV
                     FTRRFKPGG"
     gene            8544..8783
                     /locus_tag="VFU20_05780"
     CDS             8544..8783
                     /locus_tag="VFU20_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286004.1"
                     /translation="MAELFTTLTGLLVKVGIVALVANEVRGFILAAPVLYGMYLSGGT
                     VMALWLGFCSLAGIALSVAAPVFVAKKLKLIPVRA"
     gene            complement(8829..10166)
                     /locus_tag="VFU20_05785"
     CDS             complement(8829..10166)
                     /locus_tag="VFU20_05785"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5286005.1"
                     /translation="MTGPPASLAIDPPAGFPVLADSRRLRLFSVFVLYVAQGVPFGLF
                     WYAIPAWMAARGADAGDVGYVLGFTALPWSLKFVNGFVMDRYTFLPMGRRRAWLIGAQ
                     LVMVACLIACVFISPEPTDVALLAATGFIVNAATTVQDVAIDGLAVDVMHEDERARAS
                     GMMFGGQSVGIAASAAATGAITAAYGVSAAYAAAALAIALVTVAVLLVRERAGERLLP
                     WSEGRARAENLAVHAGAWWPIFRTTIVSLVRPMSLVLIPVLVLKGATYGAVTGITPLI
                     ATQTAGWAEDRVTSIAGTGQLVAGILGLTLFGYLGDRIGAKRASATALTAFVALFLAM
                     RLLVPQWGDSTVLTAFIIASIGFDVMLMISLVPIAMRLSHPAVAATQFTLYMALANFG
                     ISIGAWLVALSRDHGGAPALFVWLVIAQLAALAILLAARVPEARVDVVAPPRD"
     gene            complement(10163..>11395)
                     /locus_tag="VFU20_05790"
     CDS             complement(10163..>11395)
                     /locus_tag="VFU20_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5286006.1"
                     /translation="IYVAEHAPNDRRGASTGWIQSSAAFGLLAALLVILATRTALGED
                     AFLAWAWRIPFLASLVLLIVSVWLRFKLSESPAFAKLKEEGAVCKTPLREAFGRRENV
                     KRVLIAFFGIMCAQGAVWYCTFFYMQVFLGRSLGVPEPTVNLLLIIMTLVSAPLYVFF
                     GWLSDRVGRKPVLVGGMLLALPLYLPGFHWIAAAANPALVAAQRATPVAVATNPATCS
                     VQFDPVGTARFATACDIAKSLLVTRGISYSTVPSADGSTSVAVGDQRLPVPGGNALSD
                     GALKALKGKVGEQLGAALEAGGYPKAAAPDRLNLPVLIAVLLGFVVAATALYGPQAAA
                     LVEMFPTRVRYTAMSLPYHVGTGWVGGFLPVTSFALVAITGDIYASLWYPIAFTALSV
                     VVALLFLRETRGKPLEDV"
BASE COUNT         1934 a         3945 c         3777 g         1739 t
ORIGIN      
        1 cggcctgtac ctgctggtct cgcggggcgt cgcgcccctg gcctaccggc tggcccaccc
       61 gcaggcgctg gccaatgccc tgatcggaca aaacggcttc ctgaccgccg ccctgttcat
      121 cgccggaatg cgggcgctcg agcggcggcc gttgcttgga ggcgcaatcc tgggcctgct
      181 ggtcatcaag ccgcagttgg cggtgcttct gccaatagcc ttgctggccg gccgcgagtg
      241 gcgcgcaatc gcgggagcag ccgcgtcgtc gattgcactg ctgttgctcg cattgctgac
      301 tttcggaatg gaaacgtacc gcggcttctt cgacatcgtg ccggtctatg cgcacgcgat
      361 ggccaccagc cgctggccat ggaacgagct ggccagcgtc ttcgcgttcg cgcgccacct
      421 cggcgttccg cagcagcccg ccttgatcat ccacgcgatg gtggcgctgg gcgccacggc
      481 cctcgtgtgg agggcgtgga gcaggagaag cgagctgcgc gtgccggtgc tcgccgccgc
      541 catcctgctc gtcccgccct acctgttcac ttacgatgcc ttgctgttga tcgtccccat
      601 ggggtggctg gtcgtgcggg agcgagggcc ggtggtcgtt gccgtcgcct ggctctgctg
      661 cctgcttccg gtcgtcacct atatcggttt ctacccgggt ccgaacccga tcccgatcgc
      721 cgccttgctg tgcctgtggg cgcttcatgc cgaagagagc cggcaggcgg gcccggccgg
      781 ttccgttcat gcgcccgcct gaggagggcg cggagatcgg gccgcgccgg cgctcgccgg
      841 agtggctgtt cgcgatcctc gcgctggttg gcggaatcgt cctgatcggc ctgatcccgc
      901 cggtcggcgg cggcaatgaa cggtacaatt tccacagagc cgcaagcgcg gcgtccgcgc
      961 atctgctggt cggcccggcc ccgttgcccg gcggcattgc ggagttcctg gaggccagtc
     1021 gcgaaaaatt ccgagaaggc aggaacccgc cttattccta ctcgcgggcc gatttcgaca
     1081 ggatggcgtc gataccgtta agggcggacg agcccagggt cgtgcggccg aatccgatcg
     1141 cggtgatgca cccgatttcc taccttccgc agagcgccgc gatcgcgctc ggccggttgc
     1201 tcggcctttc ccccctcgcc ctgttctaca gtggtcgaat ctcgggtctt ctggcggggg
     1261 tcgcgctgac cttcctcgcg atccgaacga tcccggatca caggcacgcg cttgcggcgg
     1321 tcgcgctcct gccgccggtc ctgttcacgc gcagcaccct cgatgccgac cagttcacgg
     1381 tcgggctcgc cttcctgttt ctcgctctcg tgatcaggga aatcgcggct tccggacgca
     1441 ttgcgcgccg gactctggcg gtgctggcgg ccgctgcgtt catcctcgcc caatgcaaga
     1501 gcgcctacct gcttcttccg ctgctctcgc ttgccattcc cgcaagccgc ttcggctcgg
     1561 tccgagccaa ggccctcgcc tgcctgctcg tctgtgcgcc cggcatcgtg gccagcgccg
     1621 cgtggatgat cgccctgaag ttgagctact tcgacaatgt cgcgcaatat cgcacctggt
     1681 ccgggatcgt gcgccccggc gaccagctgc agttcgtcat ctccgatccg ctggcctatt
     1741 tgcggatcgt gctggcgacg gttttcgcga ctccgctcgt ccccaaggcc attctggagt
     1801 ttttcggggt gttcggccca ccggtgacgc tgccgctgcc tttttacccg gtcgcggggg
     1861 ctctccttgc gggcgtgacc ttctcgggcc gcccgatccc ggctggcccg ctgtccaatt
     1921 ggcggacgcg ggggttcgca ctggccctcg cggcggcgac catcctcgtc attctggcgc
     1981 tgctctacct ccagtggacg cggtatcgag cgccggtaat cgacgggttc caggggcgct
     2041 atctttaccc gttgataccg ttgttcctgc tgtcgattcc cgccgccggc cgggcgttcc
     2101 ttcgaattcc ggcgcgacat tggctggtgg cgctcggcct gttcagcatt gccgcgacgt
     2161 ggtggaaaac ctgggaaacc tacctcgcct gacctcgacc ggctcacgag cgggcgtcct
     2221 gcagcggcgc agggccttga agccgcggat ttcggttatg ccgggccggg tcctcgaggc
     2281 gctaacagtt cgaaccgtcc gagccgatgc agccgcttgc gtcgctcagc gccccggtga
     2341 cggcctcgcc gaggaagatc gcagccagga tgattccggt tccgacaatt gcgagaatga
     2401 ccgcatattc ggctgctgac gctccagatc catccctgag cagcgcccga agtccttcca
     2461 ccactgctcc aatcgtagat tcgttgacaa aatcgcccag cggccatcgc aggccctgcg
     2521 ctgccacggc atgcctgtca atgaccccgc ctgcacttcg ccgactgccg cttcgtattg
     2581 aaaacaatgg aagattcagg tctgcgagct tgcactgcgg atccccccgg gcggtgggcg
     2641 gaccggcggc ctcgcctggc cgagcagccg tcaagcggcg ccgtgaccgg ctaccggccg
     2701 cgcaacaaat ggtcggcgat cggcacccga accgtggacg cagcgcagcc ggcacgcccc
     2761 gcggccgact cgagcgcacg ctgtcgcccg gacggctctc ccggggcagg cgggtcgacg
     2821 aatgcgatcg agcgtcggcg gcagagtcgg gcaatctggc gcggctggac gtgagcgctc
     2881 atcaggcgga tcggacggtt ggaaaggtcg tgccgattgt gcacgaaaac ctcgtggaat
     2941 tgcgtgtcca cgatcaccac gtccgcggca atcgcgtcga tcctggcatc cgcttgcgca
     3001 attgggcgaa tgatatggtg cgccagccac ccatgcaccg gaagcagcaa cagcaatgag
     3061 gcggcgctcg tccacagcag cggccgccac aggtccttgt ggctgcgctc gagcatgtgc
     3121 cagccccatc cgccgagcag gaaagcattg ccgagcaccg ggtgaagata gcggtagccc
     3181 cagccgctac cctgccaggg cagcaggatc cagaatatca ggatcggcag cgcaaagctg
     3241 acgacaagcg cccgcacgaa gggctcgctt cgccacgcgg cccatgcgcc gaacagggca
     3301 accggggcga gaagcggatg ttgccaggtg atgaaccgaa tcaggttggc gccggtcaac
     3361 cacgcgcctt cgaggtcgac gcctttcacg gttgcgagca gtcgctcgag atagccgatt
     3421 cccgaactgg actcgatcgg gaccacggct ccagtgccca tcgagctcag caaggttggc
     3481 caggcgaccc agaatgcccc gatgcaggca taggcaaata ggtacgcgcc cagcaggctc
     3541 cattgccgcc tgccgccgag aagcgcgaga aacggagccg cgaacaatgg gtggaacagc
     3601 ggctgatgga cgcccgtggc gagaaacccg acgagcatcg ccagcgcgtg cgagcggcgg
     3661 tcgccgcgga ggaacagcat cagccagcag agattgagcg ccaggtgcgc gctcatcgcg
     3721 aacttggtca tgccggtgat caggacctgc gatgagccgg cgtagaaagc gacggtggcg
     3781 atgcgcgcgg aaaccgaatc cggccacagc cggagcgata tgcgccacag gcaccaggct
     3841 ccggcggcga ccagcaatgg agcggtaagg tcctcgtcca tcagtttgcc gacgaccgcg
     3901 tgaaaggccg cattcaccgg caaatatgcg gagatccagg cttcggcgct tccaatcgga
     3961 agcatgaaat tgcggttgag cgcattcgcc agcgatcgcc attcgggcgg gatcggccag
     4021 accagggcgc cgccgcggaa gacggcggca tcgaacgcgg ccatgcgctc gtccggggtc
     4081 gcgtcgaaat cgccgaacac caggaagcgg ccgacccagc agatcaacag cagcagcgcc
     4141 agcaagagcc acatggagcg ccgctctcgc tgcgctgtaa acagccgtcc ccggctggcc
     4201 ggacgagcat ggaaaaggca ggcggcggca aaaaatggcg cgagccaagc atcctgggtc
     4261 aggaagatgt agcgcgtgta gaggttgaat cgttcgtggg agaacgcaat cgaaccggtg
     4321 acaaggacaa ggagaaggat cgccgccgcc gcgacatatc tggcgtggtc atagccgggc
     4381 cgaacggggc gaatagacac cgacactcgc ttgctccaaa gctctcgggg gacgggactc
     4441 gtccttcaaa ggtagcggcc ttaagccgcc ggggaactct tcgtcagact ggccgcggtt
     4501 gtgcatccgc cgggccgctt ctggccagcg gccaatccca tgatgtcccg cttgtggatt
     4561 aatctggtta atccgaagct tgcgcggcga ccgcagttgt gacagcttgg cgctgcttgg
     4621 ggctcccaga ggggaattta catcatgaag atcaatattc gagtcgcggc tctggccgct
     4681 gtcgctgccg ccgcgatcgc cgcgccggcc atcgcttcgc acagctggag cacctatcac
     4741 tggcaacgga ccagcgctga gatttcgccg ccggtcatca ccgccatcga ccagaccaag
     4801 tgggggacct atgtcgtcaa cgcggtcggc gactggaacg gccaggatcc cgaccagact
     4861 ccatatatta gctatatcga gtcgccgggg ccgacggctt ccgggctcaa cccgaagact
     4921 tgcaaggccc agtccggcaa gatcctggtt tgcaatgccc gctacggctt caccggctgg
     4981 ctcggaatcg cgcagatctg gctgtcgggc ggccacatcg tccagggcat caccaagctc
     5041 aacgacagct atttcgacac cgccacctac aacaagcccg aatggcgggc gctggtcgcc
     5101 tgccaggaaa tcggccacga ctacggcctc ggccaccagg acgaggattt caacaatgcg
     5161 gacgtcgagc agccggccgg ttcgcagagc tgcatggact ataccaatca gccgctcggc
     5221 aacgaaacgc ccaacggcca cgactatgag cagctggcga tcatgtacag ccattcgcac
     5281 agcaacttca ccgcggttgg tcccgggaga tcgaactccg gaaccggcct caacaacgcc
     5341 agcggcggcg actcgccgtc cgagtggggc cgagcggtgc atttcgatgc ccagggccgc
     5401 cccgattatt tcatgatgga cctcggcggc ggcaagcgga agctgaccca cgtgttctgg
     5461 accctggagg ccaaggccgg cggccaccac ggtcactgat cgcctagtct ccgcatagca
     5521 gtccttaatt cggccggtgc cttcgggcgc cggccgtttg cttgggcggc actccgttcg
     5581 tcgtcttccg gaccggcatt tccgcgcggt ctcccgcccg cgcaaccgcc cgctctgcgc
     5641 cggcgttgac ctgctgcgat gtaccggaac gatctcacca gcagggacaa gagcggcgcg
     5701 atggcggccg tgctggcgat ccacgccgtg ctcgccttcg cactgctcca catgtcgggc
     5761 acgatcgaaa tcgacgaccc gcagcgcgcg ctgcgggtgt tcgacatcga cgagatcgtc
     5821 cccccgccgc caccgccgcc gccgccgcag cagcaggccg agcggccgaa gcccaaggag
     5881 gaggagggcg ggtcgtcgcc caacctcaag agcgaagcga cccccgtcgt cgctcccaag
     5941 cccaggatcg agcttccggt gaagccgccg atcgcggtga ccgaaactcc gcgggagggc
     6001 gcggctccga cccagggcgc ggccgacgtg cgtggccccg ggacgggcac cggcggctcc
     6061 ggcaccggca ccggcagcgg ctcgggggcg gggtcgggcg gcggcggagg aatcgccacc
     6121 cgcgcctata agctcagtcc gagcctcacg atgcgcgact acccgccgca cctgcgccgg
     6181 cgcttcccgc gcggagccgg cgtcctcgtg acgttcacgg tgctgaccaa cggccgggtg
     6241 accgattgca aggtctatca atcgagcggt gatgcagagg ttgacaatat gacctgcgct
     6301 ctggtcacga gccgcttcgt ctatcgcccc gcctataacc ggcgcggcga gcccgtcgtt
     6361 tcgcaagccg gctaccgcca gggggattga gagcagcatg atcgaagccg aatggtggga
     6421 ccatgacagc gccgaggaaa tggccgacgc ggtcgccggc gacgtcggct tcattgtcga
     6481 aagcgccctg gacgcgcgcg actcggcgct gatcgcgctt cccggcggga ccacgccgaa
     6541 gccgatctac ccgaagatcg ccgggcgcca gctggcttgg aagcgggtga cgatcatccc
     6601 gaccgacgac cggctcgcgc cgctcgacag tgagctgagc aacatccggg cgatcgccca
     6661 ggaattcctt ccgaccggcg cgcgggtgtt cccgatcgcg accaccatcg acgaccacaa
     6721 gctcgccggc aacgccgccg acgcgcggct ccaggacctg tcgtggccgc ccgacctggt
     6781 gtggctcgga atgggcgccg acgggcacac cgcgtcgatc ttcgccggcc ccgacctgca
     6841 ggacgcgctg gacgccccca aggcgcgccg cgcggtcggg gtgatgcccg acccgatgcc
     6901 cgccgacgcg ccggtgccgc gggtgaccct gacccgggcc gcaatcctgt cggcgcggac
     6961 cctgctgatc accatcaccg gcccggaaaa gcgctcgctg ctcgagcagg cgatcgccga
     7021 cggccacagc tcgaagctgc cgatcggccg cgtgctcgcc gaagccgagc agccgatcga
     7081 catccactgg tgcccatgac cgcgaggacc gcatgaaagc cctggccgcg ctcgcgcttg
     7141 ctctcgcccc gctcacttcg cttcatgcca tggcgcccga cccttatgcg cccgaaggct
     7201 tcgagggcgt ccgccacccc gcctggtcgc gcaaggcggt catctaccag gtcaacaccc
     7261 gccaattcac tccccagggg actctgaagg cggcggcgct cgagatcccg cggctgaagg
     7321 cgctcggagt cgatatcctg tggctgatgc cgatccaccc gatcggcgag aagaaccgca
     7381 agggcacgct gggcagcccg tacagcgtgc gcgattatcg cgcggtcaat ccggagctcg
     7441 gcagcttcgc cgacctcaag gcgttcgtcg acgccgcgca ccgcaacggc gtgaaggtga
     7501 tcctcgactg ggtcgccaac cacaccgcct gggaccatcc gtgggcgacc cggcacccgg
     7561 actattacga acgcgactgg aagggcagct tccgctcgac cccgtggtgg gactggtcgg
     7621 acatcatcga cctcgactac cgcaatgcgg cgatgcgccg cgagatggcg caggcgatgg
     7681 ccttctgggt gcgcgaggcg ggagtcgatg gcttccgggc cgacgtcgcc ggctatgtcc
     7741 cgcccgattt ctggaccgac gtccgccgcg acctcgaggc gatcaagccg gtgtggatgc
     7801 tcggcgagtt caaccaccgc gacctccaca tgaacgcgtt cgacgcgagc tacggctggg
     7861 cgtgggccga ggcgctgatg aagatcgcca ggggcgaggc cgacaccggc gcgctctacg
     7921 gcttctacag cgagaacgag agcgcctggc cgaccggcgc tcagcggatg atcttcacct
     7981 ccaaccacga cgagaatgcc tggtcgggca ccgagttcga gcggttcggc ccggcgctcg
     8041 ccaacgcctt cgcgctgctg ttcacgtcgg aaggaatccc gctcgtctac aacggccagg
     8101 aggccggcaa cgaccgccgc ctcaagttct tcgagcgcga cccgatcgtc tggcgcaagc
     8161 acccccacgg cgcgctgatc cgcgagtgga tccggttccg cgacgcccac ccggcgctgc
     8221 acaacgcgcc gtggggagcg cgcatggtcc atgtgaagaa cagcgagtcg cagaagatat
     8281 tcagcttcgt ccgggccagg gacggcgacg cggtgttcgt tgctcagaat tactcggccg
     8341 agccgcgcac ggcgaccctc gaggacatcc cgcacccggg gaggtgggac gagcggggcg
     8401 gtcccgccgc gactcttcag aagggcgccc ggctgactct cgcgccatgg tcgacgcggg
     8461 tgttcacgcg ccgcttcaag cccggcggct aggagcagac tccgtcaacc attttctaac
     8521 cctcgcctgc gacaaggcgg ggcatggccg agcttttcac cacgctcaca gggttgcttg
     8581 tgaaagtcgg gatcgtcgcg ctcgtcgcga acgaggtccg cggcttcatc ctcgccgcgc
     8641 cggtgctcta cggcatgtac ctgtccggcg gcacggtgat ggccctgtgg ctcggcttct
     8701 gctcgctcgc ggggatcgcc ttgtccgtcg ccgcgccggt gttcgtcgcc aaaaagctca
     8761 agctgatccc ggtccgcgcc tgaaggagcc gaatcacccg cgcgtgatcc gcaactaacg
     8821 gcccgggctc agtcgcgcgg tggggcgacg acatccacgc gcgcctccgg gacgcgtgcg
     8881 gcgagcagga tggcgagcgc cgcaagctgc gcgatcacca gccagacgaa cagcgccggc
     8941 gcgccgccat ggtcccgcga cagcgcgacc agccaggcgc cgatgctgat gccgaaattg
     9001 gcgagcgcca tgtagagcgt gaactgggtc gcggcgaccg ccgggtggct gagccgcatg
     9061 gcgatgggca cgagcgagat catcagcatc acgtcgaatc cgatcgaggc gatgatgaat
     9121 gccgtgagga cggtcgagtc gccccattgc ggcaccagca gccgcatcgc caggaacaag
     9181 gccacgaaag ccgtcagtgc ggtcgcgctc gcccgcttcg ccccgatccg gtcgccgaga
     9241 tagccgaaca gggtcaggcc gaggatcccg gccaccagct gcccggtccc ggcgatgctc
     9301 gtgacccggt cctccgccca gcccgcggtc tgggtcgcga tcagcggggt gatccccgtg
     9361 acggcgccgt aggtcgcgcc cttgagcacc aggaccggaa tcaggaccag gctcatcggc
     9421 cggaccagcg acacgatcgt ggttcggaag atcggccacc aggcacccgc atggacggcg
     9481 aggttttcgg ccctcgcccg tccctcgctc cacgggagca gccgctcgcc ggcgcgctcg
     9541 cgcaccagga ggaccgcaac ggtgacgagc gcaatggcca gcgccgcggc cgcgtaggcc
     9601 gcgctcactc cgtatgccgc ggtaatcgcg ccggtcgctg ccgccgatgc cgcgatcccg
     9661 accgactggc cgccgaacat catgccgctg gcccgcgctc gttcgtcctc gtgcatcacg
     9721 tccaccgcca ggccgtcgat cgccacgtcc tggaccgtgg tcgcggcgtt gacgatgaag
     9781 ccggtcgcgg cgagcagcgc gacgtccgtc ggctccgggc tgataaagac gcaggcgatc
     9841 aggcaggcga ccatcaccag ctgcgcgccg atcagccacg cccgccgccg ccccatcggc
     9901 aggaaggtgt agcggtccat gacgaagccg ttgacgaact tgaggctcca cggcagcgcg
     9961 gtgaagccga gcacatagcc gacatcgccg gcgtcggcgc cccgggccgc catccaggcg
    10021 gggatcgcgt accagaacag gccgaacggt accccctggg cgacatacaa gacgaagacg
    10081 gagaacagac gcagccgccg gctgtcggca agcaccggga agccggcggg cggatcgatg
    10141 gcgaggcttg ccggcggacc ggtcacacgt cttccagcgg cttgccgcgg gtctcccgga
    10201 ggaacagcaa ggcgaccacc accgacagcg cggtgaaggc gatcgggtac cacaggctcg
    10261 cgtagatatc gccggtgatc gcgaccaagg cgaagctggt gaccggcagg aagccgccga
    10321 cccagccggt cccgacgtgg tacggcagcg acatcgccgt gtagcgcacg cgcgtcggga
    10381 acatctcgac cagcgccgcc gcctgcggtc cgtagagcgc ggtcgcggcg accacgaagc
    10441 ccagcagcac cgcgatcagc accggcaggt taagccggtc gggcgcggcc gccttcgggt
    10501 agcctccggc ctcgagcgcg gccccgagct gctcgccgac cttgcccttg agcgctttca
    10561 gagcgccatc ggacaaggcg ttgccgcccg ggaccggcag cctctggtcg ccgaccgcga
    10621 cgctcgtcga gccgtcggcc gaaggcacgg tcgagtaaga gatgccgcgc gtcaccagca
    10681 ggctcttggc gatgtcgcag gcggtggcga agcgggcggt cccgaccggg tcgaactgca
    10741 ccgagcaggt cgcgggatta gtcgcgacgg ccaccggcgt cgcccgctgc gcggcgacca
    10801 gcgccgggtt ggcggcggcg gcgatccagt ggaaccccgg caggtagagc ggcagcgcga
    10861 gcagcatccc gccgaccagc accggcttgc gcccgacgcg gtcgctcagc cagccgaaga
    10921 acacgtagag cggcgcgctg accagggtca tgatgatcag cagcaaattg acggtcggct
    10981 cgggcacgcc cagcgagcgg ccgaggaaca cctgcatgta gaagaaggtg cagtaccaga
    11041 ccgcgccctg cgcgcacatg attccgaaga aggcgatcag cacgcgcttc acattctcgc
    11101 gccgcccgaa cgcctcgcgc agcggcgtct tgcacaccgc gccttcttcc ttgagcttgg
    11161 cgaaggccgg gctctccgac agcttgaagc gcagccacac tgaaacgatc agcaggacca
    11221 gcgaggcgag gaacgggatc cgccacgccc aggcgaggaa cgcgtcctcg ccgagcgcgg
    11281 tgcgcgtcgc gaggatcacc agcagcgcgg cgagcaggcc gaacgccgcc gacgactgga
    11341 tccagccggt cgacgccccg cggcggtcgt tgggcgcatg ctcggcgacg tagat
//
LOCUS       DASTZO010000208         4247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_379185,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000208 DASTZO010000000
VERSION     DASTZO010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4247
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379185"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1472
                     /locus_tag="VFU20_05795"
     CDS             <1..1472
                     /locus_tag="VFU20_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5286007.1"
                     /translation="MMGFVELLLESELDDAQRRHLGLIQSSANALLKLLNDILDISKI
                     EAGRLEIAEAPYNVRHGIKQCVRLMNLMAEQKGLSLTLAIADDFPTHLLVDGLRLRQI
                     LLNLIGNAIKFTHRGSIAVTIARGPSGDGRETLRATVADSGIGIHPDRTATIFDPFVQ
                     AELTTTRRFGGSGLGLSISRQLAEMMDGTIEVQSVLGKGTSMTLVLPLVESAAPAMPD
                     ATETAGFPNEAVPPEMRSRHASVLLVEDVDINQELFSEMLARLGHRFEIASDGAEAVD
                     LARRLQTEPDAWDLILMDLQMPVMDGLTATRAIRAFGGRAATIPIIALTASAFEEERR
                     QCEAVGMNDHLAKPVGIEALRRMINRWQGAMDTSSTVEPAGARSPVLERRIAARLRLS
                     AERLAAILEEVPTAGPDELRELLTEARTIAHVLAGTAGMVGEAALGDIAFTVEARIAA
                     GIDGETPAARDAATSAIKELIAALAGERASDGASQARTA"
     gene            complement(1500..2159)
                     /locus_tag="VFU20_05800"
     CDS             complement(1500..2159)
                     /locus_tag="VFU20_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU5286008.1"
                     /translation="MGRRLAAEAVGSFFLFATVIGSGIMAENLSAGNDAVALIGNTLA
                     TGAMLFVLITMLGPVSGAHMNPAVSLVAASRAELGRRDAAAYIAAQLAFGIFGAWAAH
                     WMFDLPALQVSVKARTGAGQWLGEGIATFGLILTILGTVRYRPQAVAASVALYITAAY
                     WFTSSTSFANPAITVARSLSDTFAGIAPANVPLFVLAQLAGAACAAVLARLLFDSDPR
                     N"
     gene            complement(2170..2682)
                     /locus_tag="VFU20_05805"
     CDS             complement(2170..2682)
                     /locus_tag="VFU20_05805"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HEU5286009.1"
                     /translation="MTGRIYNVLFLCTGNSARSILAEAAMNRAGAGRFRAFSAGSFPR
                     GEVHPMTLEVLDELGFPIEGLRSKSWDEFAAPGAPQMDFIFTDCDSAAGETCPIWPGH
                     PMTAHWGIEDPAKVEGPQRKQAFQTALTYLQARIALFMALPLGSIDEMTLANRLRAIG
                     RAQDAESAIA"
     gene            complement(2679..3134)
                     /locus_tag="VFU20_05810"
     CDS             complement(2679..3134)
                     /locus_tag="VFU20_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsI/CadI family heavy metal resistance
                     metalloenzyme"
                     /protein_id="HEU5286010.1"
                     /translation="MKRLHVHVGVSDLDRSINFYSTMFGSPPTVAKDEYAKWMLDDPR
                     VNFAISSGRHAAPGIQHLGVQAESLEELGDVYGRLRAADRPVLEEGRTTCCYAKSEKS
                     WIADPDGLVWEAFFTNGEATVYGDAPALGTLSDSLPNDKCCVPAGAVAA"
     gene            complement(3131..3472)
                     /locus_tag="VFU20_05815"
     CDS             complement(3131..3472)
                     /locus_tag="VFU20_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007014570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5286011.1"
                     /translation="MDSAMAISALGALAQEHRLALFRLLVQAGEQGMPAGRIAERLGV
                     PNSSLSFHLAQLHRAGLIKQERRHRSLIYRANYPAMNDLVAYLLENCCSGADCGADAA
                     CATPALERKTA"
     gene            complement(3592..>4247)
                     /gene="phoB"
                     /locus_tag="VFU20_05820"
     CDS             complement(3592..>4247)
                     /gene="phoB"
                     /locus_tag="VFU20_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate regulon transcriptional regulator
                     PhoB"
                     /protein_id="HEU5286012.1"
                     /translation="AELVKYHFARDGFTVTSTAVGDEALVLIDEVKPDLVILDWMIEG
                     ISGIEVCRRLRRSPATQNLPIMMLTARGEEDDRVRGLETGADDFITKPFSPKELIARA
                     NALLRRARPNLSARKLSYHGIEMDLVAHRVRRDGKPVALGPTEYRLLRHLLENPQRVF
                     SRQQLLEAVWPHSEEIELRTVDVHVRRLRLALGNESQSDLIRTVRSEGYSLDAEGAG"
BASE COUNT          719 a         1420 c         1454 g          654 t
ORIGIN      
        1 gcatgatggg cttcgtcgag ctgttgctcg aaagcgagct ggacgacgcc cagcggcgcc
       61 acctcgggct gatccagagt tccgccaacg cgctcctgaa gctgctcaac gatatcctcg
      121 acatctcgaa gatcgaggcc gggcggctcg aaatcgccga ggcgccctac aatgtccgcc
      181 acgggatcaa gcaatgcgtc cggctgatga atctgatggc cgagcaaaag gggctgtcgc
      241 tgaccctggc gatcgccgac gacttcccga cgcacctgct ggtcgacggg ctgcggctgc
      301 gccagatcct gctcaacctg atcggcaatg cgatcaagtt cacgcaccgc gggtcgatcg
      361 cggtgacgat cgcgcgcgga ccctccggcg acgggcggga aaccctgcgg gcaaccgtcg
      421 cggacagcgg catcggcatc catcccgatc ggacggcgac gatcttcgat ccattcgtgc
      481 aggcggagct gaccaccacg cgccgcttcg gcggatcggg actcgggctc agcatcagcc
      541 gccagctcgc cgagatgatg gacgggacca tcgaggtcca gagcgtgctc ggcaagggca
      601 ccagcatgac cctggtgctg ccgctggtcg aatcggcggc gcccgcaatg cccgacgcga
      661 ccgagaccgc gggcttcccc aatgaagcgg tcccgcccga gatgaggtct cggcacgcct
      721 cggtgctgct ggtcgaggat gtcgatatca accaggaatt gttcagcgaa atgctggccc
      781 ggctcgggca tcggttcgag atcgcctcgg acggagccga agccgtcgat ctggccaggc
      841 ggctccagac cgagcccgac gcctgggacc tgatcctgat ggacctgcag atgccggtga
      901 tggacgggct caccgcgacc cgggccatcc gagcgttcgg cggccgggcc gcgaccatcc
      961 cgatcatcgc cctcaccgcc agcgccttcg aggaggagcg gcggcaatgc gaggcggtcg
     1021 ggatgaacga ccatctcgcc aagccggtcg ggatcgaggc tctgcggcgg atgatcaacc
     1081 gctggcaggg cgccatggac acatcctcca ccgtcgagcc ggcgggcgcg cgaagcccgg
     1141 tcctcgagcg ccggattgcg gccaggctgc ggctgtcggc cgagcgcctg gccgctatcc
     1201 tggaagaggt tccaacggcc ggtccggacg agctcaggga gctgctgacc gaggcccgga
     1261 ccatcgccca tgtccttgcc gggaccgcgg gcatggtcgg cgaggcggcg ctgggcgaca
     1321 tcgcgttcac cgtggaagcc cggatcgcgg ccggcatcga tggcgaaacg cccgccgccc
     1381 gggacgccgc gaccagcgcg atcaaggaac tgatcgccgc cctggccggt gaacgggcca
     1441 gcgacggcgc ttctcaggcg cgcacggcct gacaaagagg cctgccagcc agcctcggct
     1501 caattgcgcg ggtcggaatc gaacagcagt ctcgccagta ccgccgcgca cgccgcgccg
     1561 gccagttggg cgagaacgaa caatggcacg ttggcggggg caatccccgc gaaggtgtcg
     1621 gacaggcttc gggcgacggt gatcgccggg ttggcgaagc tggtcgagga cgtgaaccag
     1681 taagcggcgg taatgtacag cgcgacgctc gcggctacgg cttggggccg atagcggact
     1741 gttccgagga tcgtcaggat cagcccgaag gtcgcgattc cctcgccgag ccactggccg
     1801 gcgccggtcc gcgccttcac cgacacctgc agcgccggca gatcaaacat ccagtgcgcg
     1861 gcccatgcgc cgaagatccc gaacgcgagc tgggcggcga tgtaggcggc cgcgtctcgc
     1921 cggccgagct cggcccgcga cgcggcgacc agcgataccg ccgggttcat gtgcgcgccc
     1981 gagaccggcc cgagcatcgt gatcagcacg aacagcatcg ccccggtcgc gagcgtgttg
     2041 ccgatgagcg cgaccgcgtc gttcccggcc gacaaattct cggccatgat ccccgagccg
     2101 atcacggtcg cgaacaggaa gaaggagccg accgcctccg ccgccagcct ccggcccagc
     2161 gcgggctttt caggcaattg cggattccgc gtcctgggcc cggccgatcg cgcgcaggcg
     2221 gttggccagc gtcatctcgt cgatgcttcc cagcggcagc gccatgaaca gggcgatgcg
     2281 cgcctggaga taggtgagag cggtctggaa cgcctgcttc cgctgcggcc cttcgacctt
     2341 cgccggatcc tcgatccccc agtgggcggt catcggatgg cccggccaga tcgggcaggt
     2401 ctcgccggcc gctgaatcgc agtcggtgaa gatgaaatcc atttgcggag cgcccggggc
     2461 ggcgaactcg tcccagctct tggagcgcag gccctcgatt ggaaagccga gctcgtccag
     2521 gacctccagg gtcatggggt ggacctcgcc gcgcgggaag ctgccggccg aaaacgcccg
     2581 gaaacgccct gccccggcgc ggttcatcgc cgcttcggcg aggatcgagc gcgccgaatt
     2641 gccggtgcac aggaacagca cattgtagat ccggccggtc atgccgcgac ggctcccgcc
     2701 gggacgcagc acttgtcgtt gggaaggctg tcggagagcg tgcccaatgc aggtgcgtcg
     2761 ccgtagaccg tcgcttcgcc gttggtgaag aaggcctccc acaccaggcc gtccgggtcg
     2821 gcgatccagc tcttctcgct cttcgcgtag cagcaggtcg tgcggccttc ctcgagcacg
     2881 gggcggtccg cggcgcgaag ccggccatag acgtcgccca gctcttcgag gctttccgcc
     2941 tggacaccca gatgctggat gcccggagcc gcatgccggc cgctcgagat ggcgaaattg
     3001 acgcgcgggt cgtcgagcat ccacttggcg tattcatcct tcgccacggt cggcgggctg
     3061 ccgaacatcg ttgagtagaa attgatcgag cgatcgaggt cgctgacccc gacgtggacg
     3121 tgcaggcgct tcatgcggtt ttcctttcga gagccggggt tgcgcaggcc gcatcggcgc
     3181 cgcagtcggc cccggagcag caattctcca ggaggtaagc gaccaggtcg ttcatcgcgg
     3241 gatagttggc gcggtagatc agcgaacggt ggcgccgctc ctgcttgatg aggcccgcgc
     3301 gatgcagctg cgcgagatgg aaggacagcg aggagttggg gactccgagc cgctccgcga
     3361 tccgtccggc cggcatgcct tgctccccag cctggacgag cagccggaac agggcgaggc
     3421 gatgctcctg ggcaagtgcg ccgagcgccg aaatcgccat ggccgaatcc aagttgaacc
     3481 tcctcgtttc gatgaaactc gaaatgtcac gcgagtgacc gcgagtcaag cacctttcga
     3541 tagatttgga attgaggaac cttgaacggc caagattcgt gcgcgaagcg cctagccggc
     3601 gccctcggca tcgagcgaat aaccctcgga gcgcaccgtc cggatcaggt cggactgcga
     3661 ttcatttccc agcgccaggc gcagccggcg gacgtggacg tcgaccgtcc gaagctcgat
     3721 ttcctcgctg tgcggccaga ccgcttccag cagctgctgg cgcgagaaca cccgctgcgg
     3781 attctcgagc aagtggcgga gcagccggta ttcggtcggc ccgagcgcga ccggcttgcc
     3841 gtcgcggcgg acgcggtggg cgaccaggtc catttcgatc ccgtgatagc tcagtttcct
     3901 ggccgacaaa ttggggcgcg cccggcgcag aagcgcgttg gcccgggcga tcagctcctt
     3961 ggggctgaac ggcttggtga tgaaatcgtc ggcgccggtc tccagcccgc gcacccggtc
     4021 gtcctcttcg ccgcgcgcgg tcagcatcat gatcggcaga ttctgggtcg ccgggcttcg
     4081 ccgcagccgg cggcagacct cgatgccgct gatcccctcg atcatccagt cgaggatcac
     4141 caggtcgggc ttgacctcgt cgatcaggac cagggcctcg tcgccgaccg cggtgctggt
     4201 gacggtgaac ccgtcgcggg cgaaatggta tttgaccagc tcggcga
//
LOCUS       DASTZO010000209         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_196609,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000209 DASTZO010000000
VERSION     DASTZO010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196609"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="VFU20_05825"
     CDS             <1..1223
                     /locus_tag="VFU20_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HEU5286013.1"
                     /translation="SLVLGLGIGALAAKLGDGIREPALQASNVVGGLWLNALKMTVIP
                     LVVALLVVGIARGAEAARAGRIAGRSVLWIVIVCTASAIFGMVMIQLLTGLFPLPPAA
                     AEALQAGLAGLDQAATAQMPGIADFFRGVVPENVIAAASNGDVLPLVVFALLFALATT
                     RIGQQRRRSLVGLFEAISDTLLVIIGWVLWIAPIGVFALSFTVGASAGGAAFAGVLHY
                     IVLVSAIGVAVTLAGYPIATLAGRVGLGSFARAMIAPQSVAVSTRSSLASLPAMLAAA
                     RLMGIREQVADVTLPIAVALFRGTGPAMNVAVAFYVAHWLGYEPSLGQVIAATAVGAV
                     MSYAAISLPGEITFISSIAPIAMALGVPIAPLALLVAVEMVPDIFRTLGNVTLDVAVT
                     AAVDRSAGGEAPEA"
     gene            complement(1576..2322)
                     /locus_tag="VFU20_05830"
     CDS             complement(1576..2322)
                     /locus_tag="VFU20_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU5286014.1"
                     /translation="MDDELKVLIADDEPLAAERLQLLLAKCSGIDLVGTASDGESAVR
                     MARALEPDVLLLDIAMPGLDGIDVARAIAGPGGSPAVVFVTAFDQFAVAAFEVAAVDY
                     LMKPVDPERLERALGRARAYVRQRREEPGRAPPKASQWLDEFWASDLTGLVRIAARDV
                     DRVSAERDYMRLHVGRRSWLIHHSMTALEEGLDPELFVRLHRSAIVRRDFITGFTRNQ
                     SGRWIARLADGGEQPVGRLYSDRVRTIAGR"
     gene            complement(2315..>2974)
                     /locus_tag="VFU20_05835"
     CDS             complement(2315..>2974)
                     /locus_tag="VFU20_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5286015.1"
                     /translation="EALGARRRVAEAESAARAAQVRALRYQVNPHFLFNTLNSLSSLV
                     MSGRPQEAEEMILKLSTFFRSSLSLDPSADVTLAEEIALQRLYLDIERVRFPKRLKVE
                     IDVPAELETALLPALVLQPVVENAIKYGVSPTRDKVVLRIAAREAGPGRFTVEISNNG
                     KPGSARERDDTPGGTGVGLGNVRERLRARFGNAAQCEFGPIEGGGYRVVMTLPLDRAD
                     G"
BASE COUNT          389 a         1064 c         1053 g          468 t
ORIGIN      
        1 tctcgctggt cctcgggctc ggcatcggcg cccttgccgc gaagctcggc gacggcatcc
       61 gcgagccggc gctgcaggca tccaacgtcg tcggcgggct gtggctcaac gcgctcaaga
      121 tgacggtcat cccgctcgtc gtcgcgcttc tggtggtcgg catcgcgcgg ggcgcggagg
      181 ccgcccgggc gggccggatc gccggccgct cggtgctgtg gatcgtcatc gtctgcaccg
      241 cctcggcgat cttcgggatg gtgatgatcc agctgctcac cggcctgttc ccgctgccgc
      301 cggcggccgc cgaggcgctg caggcggggc tcgccgggct cgaccaggcg gcgacggcgc
      361 aaatgccggg gatcgccgat ttcttccgcg gcgtcgtgcc cgagaacgtg atcgccgccg
      421 cctccaacgg cgacgtcctg ccgctggtcg tcttcgcctt gctgttcgcg ctcgccacga
      481 cccgcatcgg gcagcagcgc cgacgctcgc tggtcggcct gttcgaggcg atctcggaca
      541 ccttgctggt gattatcggc tgggtgctgt ggatcgcacc gatcggggtc ttcgccctgt
      601 cgttcaccgt cggcgcgagc gccgggggag cggcctttgc cggagtgctg cattatatcg
      661 tgctggtgtc ggcgatcggc gtcgcggtga cgcttgccgg ctatccgatc gcaaccttgg
      721 ccggccgtgt cggcctgggc agcttcgccc gggcgatgat cgcgccgcag agcgtcgccg
      781 tctcgacccg ctcgtcgctg gcgtcgcttc cggcgatgct cgccgcggcg cggctgatgg
      841 ggatccgcga gcaggttgcc gacgtcacct tgccgatcgc ggtcgccttg ttccgcggga
      901 ccgggccggc gatgaacgtc gcggtcgcct tctacgtcgc ccactggctc gggtacgagc
      961 cgagcctcgg ccaggtgatc gccgcgaccg cggtcggagc ggtgatgagc tacgccgcga
     1021 ttagcctgcc cggcgagatc accttcatca gctcgatcgc cccgatcgcc atggcgctgg
     1081 gcgtgccgat cgcaccgctc gccctgctcg tcgcggtcga gatggtcccc gacattttcc
     1141 ggacgctcgg caacgtcacc ctcgacgtcg cggtgaccgc ggcggtcgac cgatcggccg
     1201 gcggcgaggc gcccgaagcc tgaacgccag ctgaccgccg ttcgtcgatt cggccacgca
     1261 ccaacggcgt tcgtcgctgg ggcgcaacga tcgccgaacc tcgccccgcg ctggtcgagc
     1321 ggcggcggtt ttccgcgccg cccgcggcta gcagggaatg gcggcgcggc cctttatccc
     1381 caggagggcc ggcgccgcgg ttcgaaggag cgtgacgatg aagatcttga cgatgctggc
     1441 ggcgacgatc gtttcggcga tcctggtcct gccgacggtc gcccaggccg agttgtcggg
     1501 cctcgttgcc tgaacggcag tgcagcccga cccgaagcgc ccggctgccc gccccaggcg
     1561 gccgggcgcg tttgcttatc gcccggcgat ggtcctgacc cggtccgaat agagccggcc
     1621 gaccggctgc tcgccgccgt cggcgaggcg cgcgatccag cggccggact ggttgcgggt
     1681 gaagccggtg atgaaatccc gccgaacgat cgccgagcgg tgcaggcgca cgaacagctc
     1741 ggggtcgagg ccctcctcga gcgcggtcat cgaatgatgg atcagccagc ttcggcgccc
     1801 gacgtgaagc cgcatgtagt cgcgctcggc cgacacccgg tcgacgtcgc gggcggcgat
     1861 ccggaccagg ccggtcaggt ccgaggccca gaactcgtcg agccattgcg aggccttggg
     1921 cggcgcccgc ccgggctcct cgcggcgttg ccggacatag gcccgggccc ggccgagcgc
     1981 ccgttcgagg cgctccgggt cgaccggctt catcagataa tcgaccgccg cgacctcgaa
     2041 cgcggcgacc gcgaactggt cgaacgcggt cacgaacacg accgccggcg agccgcccgg
     2101 accggcgatc gcccgggcga cgtcgatccc gtcgaggccc ggcatggcga tgtcgagcag
     2161 gagcacgtcg ggctcgagag cgcgggccat ccggaccgcg ctctcgccgt cgctcgccgt
     2221 cccgaccagg tcgatccccg agcatttggc gagcagcagc tgcagccgct cggcggccag
     2281 cggctcgtcg tcggcgatca ggacctttag ctcatcatcc atcggcccga tccagcggca
     2341 gggtcatcac gacacgatag ccgccgccct cgatcggccc aaactcgcat tgcgcggcat
     2401 tgccgaaccg ggcccgcagc cgttcgcgga cgttgccgag gccgaccccg gtgccgccgg
     2461 gggtgtcgtc gcgctcccgc gccgagcccg gcttgccatt gttgctgatc tcgaccgtga
     2521 atcggccggg cccggcctcc cgcgcggcga tccgaagcac gaccttgtcg cgcgtcggcg
     2581 agaccccgta cttgatcgca ttctcgacca ccggctgcag gaccagcgcc ggcaggagcg
     2641 cggtctcgag ctccgccggg acgtcgatct cgaccttgag gcgcttgggg aagcgcaccc
     2701 gctcgatgtc caggtacagg cgctggagcg cgatctcctc ggccagagtg acgtcggccg
     2761 acgggtcgag cgacaggctc gaacggaaga aggtcgacag cttgaggatc atctcctcgg
     2821 cttcctgcgg ccgcccggac atgaccagcg acgagagcga attcagagtg ttgaacagga
     2881 aatgcgggtt gacctggtac cgcagcgccc ggacctgcgc ggcgcgggcg gcgctttcgg
     2941 cttcggcgac gcgccgccgt gcgccgagcg cttc
//
LOCUS       DASTZO010000210         3385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_65999,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000210 DASTZO010000000
VERSION     DASTZO010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3385
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65999"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..995
                     /locus_tag="VFU20_05840"
     CDS             234..995
                     /locus_tag="VFU20_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5286016.1"
                     /translation="MGKGRLSGKVAIVTGAASGIGLAAAELFRSEGAEVVGSDIAAAD
                     GIVQADAGSEDDVRSLVEHAVWKHGGLDVMFANAGISGGLASIFDQSAEDWAEILRVD
                     LIGPFLAIKHSAPALKARGGGSIICTASVAGLRSGAGGAAYSAAKAGVISLVQTAANQ
                     LAGTNIRVNAICPGLIETGMTELIYRTAREAGQDHRIGELNPLRRGGEASEIAGAALF
                     LASDESSYVNGQALVVDGGLTSSMPTARRFDIRAE"
     gene            1085..1708
                     /locus_tag="VFU20_05845"
     CDS             1085..1708
                     /locus_tag="VFU20_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286017.1"
                     /translation="MQLDLSQLVKLVGELPDALAKTVRYGVEAWDGTSARTAQRRLRN
                     MSVSLRNLIISNVGAGNELEDYTRIWKEVTKNGHRVPSAAGKSHLESAWIGVFGPLEA
                     ALEDGYSVLASVQKERSGFVITEGYHNLLTTLRTRAKLVQQLRGLPAPVTVDEMKEFR
                     NFLVNYSRLRSSVEQCLQFLNEYAQTAELRSKSASGAQGLPLNKPAS"
     gene            1952..3166
                     /locus_tag="VFU20_05850"
     CDS             1952..3166
                     /locus_tag="VFU20_05850"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU5286018.1"
                     /translation="MNRMMIVAAMAAGLAACDRQAPAPAGDEGAETADSTDVDFDAIA
                     RRVVTESAGVKPGEVVVINGSIAEPRLLEALQAAVLLAGGHPIVAISFPRANKRALTQ
                     APMEYLRQPPRADLAMLEAGDVFISAGAVEDPELFSDVDEARLNAVREANQAAGEAAA
                     RKRARSVDIGQAGGIPTPAYAKSRNADYKAMRAMFFRALAVPSNVIAQRGAVVSGKMQ
                     PGQQVRLRSSSGTDLTFKLAAGRSRVSTGRAADNDTGQGMASAFLPAGDFYACVDPAS
                     ANGVLVSPADRFRGKPVRNLRVTFQNGAITSITADEEADALQRYFAELDEGGKRMSLI
                     NIGLNPESRPLKGSDYMSWEMSGVPTVVVGNAMWAGCAHGGEASQQFHQLGATLAAGS
                     AEVIKDGNLVVN"
BASE COUNT          596 a         1117 c         1120 g          552 t
ORIGIN      
        1 ccccgccgag cgcgctttcg ccttcaattc ctcgatcacc ggcagcactt tccaccggtc
       61 gccctcgcgc tgctgcgagt cgtaatcggc gcagcgcagc cgcacctcgc aatccatgaa
      121 tgccttgacc cggtcgcgcc actcgacgtc ggctggcgat agttcgaaat ccatggcgcg
      181 gctccggatg gcgtggcagc tataacagcg gcaattggct atagggagcg gccatgggca
      241 aagggcgcct cagcggcaag gtcgcgatcg tcaccggcgc cgcgtcgggc atcggcctcg
      301 cggcggccga gctgttccgg agcgagggcg ccgaggtcgt cggctcggac atcgccgccg
      361 ccgacggcat cgttcaggcc gacgccggca gcgaggacga cgtccggagc ctcgtcgagc
      421 atgcggtctg gaagcacggc ggcctcgacg tgatgttcgc caacgccggc atttcgggcg
      481 gactcgcgtc gatcttcgac cagagcgccg aggactgggc cgagatattg cgggtcgacc
      541 tgatcggccc gttcctcgcc atcaagcatt cggcgccggc attgaaagcg cgcggcggcg
      601 gctcgatcat ctgcacggcc agcgtcgccg ggcttcgctc gggcgccggc ggggcggcct
      661 attcggcggc caaggcgggg gtgatcagcc tcgtccagac cgccgccaac cagctcgccg
      721 gaaccaacat aagggtaaac gcgatctgcc cggggctgat cgaaaccggc atgaccgagc
      781 tcatctaccg aacggcgcgc gaggccggcc aggaccaccg gatcggcgaa ttgaacccgc
      841 tgcggcgcgg cggcgaggcc agcgagatcg ccggcgcggc gctgttcctc gcatcggacg
      901 agagctccta cgtcaacggc caggcgctgg tcgtcgacgg cggcctcacc agcagcatgc
      961 cgaccgcccg ccgcttcgac atccgcgcgg agtgacgggc gcagcctgct ccgggggagc
     1021 gggcgagcac gttaccgtca actgcgcgcg atcggacgct cgcatgttcg cgaaaggacg
     1081 cggtatgcaa cttgacctaa gccaactcgt caaactggtg ggtgaactgc cagacgcgct
     1141 tgcgaagaca gtacgatacg gagtcgaagc ctgggacggc accagcgcac ggacggctca
     1201 acgacgcctt agaaatatgt ctgtaagcct tcgcaatctc atcatttcaa acgtcggcgc
     1261 tggtaatgag ctggaagact acacgcgtat ttggaaagag gtaacgaaga atggccatcg
     1321 cgttcccagc gccgctggca aaagtcatct tgagagtgcg tggattggtg tgtttgggcc
     1381 gttggaagcc gcccttgagg acggctacag cgtgcttgcc agtgtccaga aagagcgcag
     1441 cgggttcgtg ataacggaag ggtaccacaa cctcttaacc actcttcgaa ctcgtgcaaa
     1501 actcgtgcaa caacttaggg gcttgcctgc gccggtcact gtcgacgaga tgaaggagtt
     1561 tagaaacttc cttgtcaatt acagtcggct gcgtagcagt gttgaacagt gcctacagtt
     1621 cctgaatgag tatgcccaga cggcggaact ccggagtaaa tccgcgtccg gcgcgcaggg
     1681 cttgccactg aacaaaccag catcctaggg cgggtaggat gccgcgcgtt tagcattgca
     1741 gctcgcaatg gctcgacgcc gacattcgtt gccaatccaa ctgttgcacc gcctcgacat
     1801 cgcgccgaac gacgtcaact ttcgtcaatt ttgccgcggt ctcgactttg tgattgtctc
     1861 gccgctcgga cgactccgcg ccaagttgca tttcggggcg aggctttcct gcggcttcca
     1921 caggggcggc cgatctcaac gaggggtctt tatgaacagg atgatgatcg tcgccgcgat
     1981 ggcggccggg cttgccgcgt gtgaccgcca ggcaccagct ccggcgggcg acgagggtgc
     2041 cgaaaccgcc gattcgacgg acgtcgattt cgacgcgatc gcaaggcgcg tcgtcaccga
     2101 gtccgccggc gtcaagccgg gcgaggtcgt ggtcatcaac ggctccatcg ccgagcccag
     2161 gctgctcgag gcgctgcagg ccgcggtgct cctggccggc ggccatccga tcgtcgcgat
     2221 cagtttcccg cgggcgaaca agcgcgcgct cacccaggcg ccgatggaat atctccgcca
     2281 gccgccgcgg gccgaccttg ccatgctcga ggccggcgac gtgttcattt ccgccggcgc
     2341 ggtcgaggat ccggagctgt tctccgacgt cgacgaagcg cgcctcaacg cggttcgcga
     2401 agccaaccag gcggccggcg aagccgcggc ccgcaagcgc gcgcgctcgg tcgacatcgg
     2461 ccaggccggc ggcatcccga ccccggccta cgccaagagc cgcaacgccg attacaaggc
     2521 gatgcgggcg atgttcttcc gagcgctcgc ggttccctcg aacgtcatcg cccagcgcgg
     2581 cgcggtcgtc tcgggcaaga tgcagccggg ccagcaggtc cgcctccgct cgtcgtcggg
     2641 aaccgacctg accttcaagc tcgccgcagg ccgctcgcgg gtcagcaccg gccgggcggc
     2701 ggacaacgac acgggacagg gcatggcgtc ggccttcctg cccgccggcg atttctacgc
     2761 ctgcgtcgat ccggcgtcgg ccaacggagt cctggtgtcg cccgccgacc gcttccgcgg
     2821 caagccggtc cgcaacctcc gagtcacatt ccagaatggc gccatcacct cgatcaccgc
     2881 cgatgaggag gcagacgcgc tccagcgcta tttcgccgag ctcgacgagg gcggcaagag
     2941 gatgtcgctg atcaacatcg gcctcaaccc ggaaagccgc ccgctcaagg gctccgacta
     3001 tatgtcgtgg gagatgagcg gggtgccgac ggtcgtcgtc ggcaacgcca tgtgggccgg
     3061 atgcgcgcac ggcggcgaag ccagccagca gttccaccag cttggagcga cgctcgccgc
     3121 cggcagtgcc gaagtcatca aggacgggaa cctggtcgtc aattgaggag cgcgggcggt
     3181 gcctgggccg cttcccgggc atccctcgtg aatacgtcta tgcctagcgc cgccgcttcc
     3241 tcgccggcgg cggcggcggc ggcgggcgca ggtcggggtt gcggatgcaa ttgttgaacc
     3301 cgacccagtc cttgcgggtg ccgttgcgcg acgagttgcg gttgcacagc tggatcttcc
     3361 acgccggcaa ggaataggtc ttcac
//
LOCUS       DASTZO010000211        14831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_932195,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000211 DASTZO010000000
VERSION     DASTZO010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 14831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14831
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_932195"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..562
                     /gene="ruvA"
                     /locus_tag="VFU20_05855"
     CDS             <1..562
                     /gene="ruvA"
                     /locus_tag="VFU20_05855"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU5286019.1"
                     /translation="GADWAVIDVAGVGYAVLASARTLDALGPVGGEVMLLTEMQVRED
                     SLTLFGFATAGEREAFRALTSVQGVGGRVALAILSALGPDELSSAVASGDKAMIARAQ
                     GVGPKLAARIANELQGKLGAPGLAGTAGAALPRTGTAADAVSALGNLGFKPAEASSAV
                     AAAQDELGPDAELDALVRLALRKAAK"
     gene            601..936
                     /locus_tag="VFU20_05860"
     CDS             601..936
                     /locus_tag="VFU20_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286020.1"
                     /translation="MIVIAYGLAVLTTMLQAGEPNAFWWWLLFFPFYAWWLAPVGLPL
                     FYPWPSWFITLPIGAMAAYSSHIYERDMFGPGARSTSALIFIFLPIYQWMAVGVLIGV
                     AWLTRRLTK"
     gene            933..1334
                     /locus_tag="VFU20_05865"
     CDS             933..1334
                     /locus_tag="VFU20_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU5286021.1"
                     /translation="MTTRTASCRCGQLRVTASSEPVRVSACHCLDCKKRSGSAFAVQA
                     RWPADRVTIQGRSKSWVNVADSGNQITFHFCPDCGSDVHYEINGKFDGLIAIPLGAFE
                     DPYFASPAFSVWEERKHEWVEILGDQVEHLD"
     gene            1334..2377
                     /gene="ruvB"
                     /locus_tag="VFU20_05870"
     CDS             1334..2377
                     /gene="ruvB"
                     /locus_tag="VFU20_05870"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU5286022.1"
                     /translation="MATDPARITSPERTPEDADAALRPKSLDEFIGQQGARENLRVFV
                     NAAKSRGEPLDHVLLHGPPGLGKTTLAGILAREMGVGFRATSGPVIAKSGDLAALLTN
                     LEDGDVLFIDEIHRLQPAIEEVLYPAMEDRALDLMIGEGPSARSVRIDLPRFTLIGAT
                     TRQGLLTTPLRDRFGIPIRLNFYSVPELEQVVRRAAGLLGVDLKDDGAHEIARRSRGT
                     PRVAGRLLRRVRDFAHAVGAQAIDAKVADQALDRLEIDGLGLDAMDRRYLTMIADIYG
                     GGPVGIETLAAGLSEPRDTIEDVIEPFLIQLGIIARTARGRCLNGRGWKHLGLTPPAG
                     SQSGLFDQGAAPK"
     gene            2374..3519
                     /locus_tag="VFU20_05875"
     CDS             2374..3519
                     /locus_tag="VFU20_05875"
                     /inference="COORDINATES: protein motif:HMM:NF022732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3089 domain-containing protein"
                     /protein_id="HEU5286023.1"
                     /translation="MIRVAMLVSLAAFAAAPAAAQPPAPAPDYSKPASWLCLPGRTDV
                     CSQPLPTTALNPNGYGPVGNSRPPPTPPPVDCFYVYPTVSRDVGMNSDLVPDASERSA
                     AQSQFARFAGVCRTFAPLYRQLTVSAIAASAVGTDLTAPSALAYRDVAAAWRRYLAAH
                     NKGRPFVLVGHSQGSAMLQELIRREIEGRPIARQMLRAIIPGFNVLVPQGRRVGGSFK
                     STPLCASPSETGCVMSWVSYRENNVPPEGAMFGWAPQPGMTVGCTNPARPGSTRWERL
                     DSYWNTRLSLPVPGGPIVWSSQGSPPTQFLRTEGLVSARCVNAGRLGYLSVRTNADPS
                     DKRTDRIGGEVGMLGMFIPGWGMHLADLAIAQGDLVRTVEELGRAKR"
     gene            3566..4021
                     /locus_tag="VFU20_05880"
     CDS             3566..4021
                     /locus_tag="VFU20_05880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgC/FadM family acyl-CoA thioesterase"
                     /protein_id="HEU5286024.1"
                     /translation="MTPSPFDQPYRGGFVGPEHHFALTVYFEDTDTAGIVYYANYLKF
                     MERARSDMIRAAGVDQNSAHHSGGGAYAVAEVDIRYLKPARLGDDLVVVSTVDQVRAV
                     SVLIHQRVMRGPELLTDARVTAAFLTADGRPQRQPKEWVERFKAIGEGQ"
     gene            4042..5319
                     /gene="tolB"
                     /locus_tag="VFU20_05885"
     CDS             4042..5319
                     /gene="tolB"
                     /locus_tag="VFU20_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HEU5286025.1"
                     /translation="MAVLLAALGLPAMAAAQDKPPAVIAVPQLATPENVSTPAGDTGL
                     IARYVADVIASDLRSTRELVPIGPENSRVYSYPESTAPSFSLWRKTGAKTLVTGFVQS
                     RPDGRMTVACYLHDLAAGREIARKGFVVAPAEWRRAAHRCSDAVYERLAGRPGWFDTR
                     IAYVAESGGGAGRVKRIAVMDSDGTAHRYVTAGDSTVLTPRLAPGGETIAYVSFAGGQ
                     PHIRLADAEGGQDRPLLPGTAMSFAPSFSADGKRILFSMAMDGNTDVYVADADGGALQ
                     RLTSTPGVDTAPSFSPDGSRIVFESDRSGSQQLYVMNADGSEPRRISFGGSAYASPKW
                     SPDGEWVAFTRIAGGTLGIGVMSPAGTGERVVTTGAHDERPSWSAGGRDLLFQRSEGG
                     RTGLYAVSVDGGAPRRIVTPQDGSDPDWSTPGS"
     gene            5322..5801
                     /locus_tag="VFU20_05890"
     CDS             5322..5801
                     /locus_tag="VFU20_05890"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU5286026.1"
                     /translation="MRRTILMVLALLALGGAPLAAAQVMRGPPPGLLPPPVTIDMLQS
                     DLVAKTGSDTVYFAGNGHGINAAGQATLSAQARWLLANPAVRARIEGHADDRTPRDYA
                     LAVGERRADAVRDFLVLQGVPAAQLTIISWGKERPAVGGSSEMALALNRRARTVLMP"
     gene            complement(5798..6100)
                     /locus_tag="VFU20_05895"
     CDS             complement(5798..6100)
                     /locus_tag="VFU20_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HEU5286027.1"
                     /translation="MGAISQKGAEFLIRSRLADAERGDVEALFELGVTYSTGRGGAPV
                     DLIEAHKWFNLAALSGCTRGQQCRAEIAIEMTAREIAEAQRQARAWLGAGQQRHAA"
     gene            complement(6171..7046)
                     /gene="panC"
                     /locus_tag="VFU20_05900"
     CDS             complement(6171..7046)
                     /gene="panC"
                     /locus_tag="VFU20_05900"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEU5286028.1"
                     /translation="MQIIREIGALPGEVFALRSAPRDNRTASIALVPTMGALHAGHMA
                     LVAEAKRLADRVVATIFVNPMQFGANEDLDRYPRREAADAEMLEQAGCDLLWIPSVGD
                     IYPEGFATKVSVSGLGDRWEGEARPGHFDGVATVVARLLLAVRPDVALFGEKDFQQLA
                     VIRRMVADLGLGVEIAGVPTVRDPDGLALSSRNAYLSADERKRALALPQALAAARDAI
                     LSGETAEAALSAARQALRNAGFSTVDYVALVDAATLEPIDEPRGAMRLIAAATIGGTR
                     LIDNLAVEPASPQQP"
     gene            7118..7918
                     /locus_tag="VFU20_05905"
     CDS             7118..7918
                     /locus_tag="VFU20_05905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HEU5286029.1"
                     /translation="MSDPHFIIFANEKGGTGKSTTAVHTAIALAASGHRVAALDLDAR
                     QRTMARYLENRDATMRRLDKQLPHARFEVLDDHTETGLAAAVARLSEDVDVIVVDTPG
                     RDDPVSRAAILEADTLVTPMNDSFVDLDLIGQVHPENYKITKPSFYAELIWNSRTQRA
                     KATGRSVDWVVLRNRLQHVDSHNLRRVGAALDELARRVGFRVIPGLGERVIYRELFPK
                     GLTLLDFEHLGEVGLGHITARQELREMIAGLGIPEPGASVPEPVAAAG"
     gene            7951..9330
                     /locus_tag="VFU20_05910"
     CDS             7951..9330
                     /locus_tag="VFU20_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEU5286030.1"
                     /translation="MSHRFHASVLREYDVRGIVGDTLTEADALALGRSYAALAKEEGA
                     ARVAVGRDGRTHSPGLEAALVDGLTQSGLDVVRIGMGPSPMLYFATATLGVDGGIQVT
                     GSHNPADYNGFKLLLKGRSVFGEEIQALGRRAAAGDWNEGSGTVDDADVLDSYADRLV
                     ADFAGGPFRVGWDAGNGAAGPVLEKLIERLPGEHHTLFTAVDGTFPNHHPDPTVEANL
                     ADLKALVAAKGLDFGIAFDGDGDRIGAVDGKGRVIWGDQLLAILAGPVLAECPGATII
                     ADVKASQLLFDRIAELGGRPLMWKTGHSLIKAKMKETGAPLAGEMSGHIFFAHRWYGF
                     DDALYAGVRLIEAVAESGKSLTELMDAMPRSVATPELRFPVEASRKFAVIDEVLERLS
                     AEGAIVDGTDGARVSTEDGWWLLRASNTQDVLVARAEAKDEAGLARLVAQIDKQLAKS
                     GVMRTEVAH"
     gene            complement(9340..10338)
                     /locus_tag="VFU20_05915"
     CDS             complement(9340..10338)
                     /locus_tag="VFU20_05915"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     exonuclease"
                     /protein_id="HEU5286031.1"
                     /translation="MARLGSWIEPSPEGIYVRPADAWVDPSEPKARALITHGHGDHAR
                     GGHDEVWATPETLAIMAVRYGPQPGQGIAYDETVRLGEVDIRFVPAGHVLGSAQIVLE
                     HAGERVVVSGDYKRRDDPTCAPFVPVPCDIFITEATFGLPVFRHPDTASEIDRLLHRL
                     HSDPSRCVLVGAYALGKAQRVIAELRARGHREPIYYHGALERLCRLYEQFGVELGELR
                     HTAGATKQEIAGHIVLCPPGALNDRWSRRLPDPVTAMASGWMRIRQRARQRNIELPLV
                     ISDHADWDELTQTIRDVAPREVWITHGREDALMHWCMTHQIKARELNLVGYEDEDD"
     gene            10419..10613
                     /locus_tag="VFU20_05920"
     CDS             10419..10613
                     /locus_tag="VFU20_05920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286032.1"
                     /translation="MDETLGSLMTIIGPIILLVLLVWLVMRSRRRSGRPTDTTAQTEQ
                     ATDRLYDDEERRRREGTDDL"
     gene            10610..13024
                     /locus_tag="VFU20_05925"
     CDS             10610..13024
                     /locus_tag="VFU20_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response DEXH box
                     helicase"
                     /protein_id="HEU5286033.1"
                     /translation="MSASALPEIVRRWFEGRGWEPRRHQLAMLDLARARRSGLLVAAT
                     GAGKTLAGFLPTICDLIEHPAEGLHTLYVSPLKALGVDVQRNLLGPIGEMGLAIRVET
                     RSGDTPSDRKARQRVRPPHILLTTPESLSLLLSYPESATLFAGLKTIVVDELHAFAKE
                     KRGDLLALAMARLQTFALGLRRVGLSATISDPDAYRGWLAPDADETQVDLVTGDPGAE
                     PDLSILIPDGKIPWSGHSGRHAAREVMELIERHKMTLIFCNTRSLAELIFQDLWAVND
                     SALPIGIHHGSLALEARRKVEAAMSSGKLRGLVATASLDLGIDWGDIDLVVQMGAPKG
                     SSRLLQRIGRANHRLDEPSKGIIVPGNRFEYLEARAALDAIDDGELDPENFRAGALDV
                     LAQHILALACSAPFEEAELLAELRSAAPHAGLRDETFTEVLNFIATGGYALKAYDRFR
                     RLVREPEGRWRIARPGIAAQHRLNAGVIVEQPLLTVRFRGGRKLGTIEEGYASTLAPG
                     DHFYFAGLSLEVEQFKDTDIIVHASAKTARIVTYGGQRMSMSTHLANRVRSMLADRND
                     WHRFPDDVREWLEVQDRRSTLPEPEQLLVETFEHHGQHYLVAYSFEGWNAHQSLGMLI
                     TKRMENMGLKPLGFVANDYGLACYGLEPIRDPKALFSPDILEQEFVDWVEQSYLLKSA
                     FREVAVIGGLVERHHPGKRKSGRQVSFSTDLIYDVLRRYEPQHLLLRAAWDDARARMT
                     ELGRLVRLVDRASATMVHVEADRITPMAVPLMVIVGREAMPPGGEADETLLIQAESLA
                     REAMAV"
     gene            13071..13322
                     /locus_tag="VFU20_05930"
     CDS             13071..13322
                     /locus_tag="VFU20_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016747863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286034.1"
                     /translation="MKALVLTAAATALSGCVVADVAETAVGVAKIPVKVASAGVDAAT
                     TSQSEADEKRGRELRKADEKRAREEREAAKKAAERPQSE"
     gene            13334..13786
                     /locus_tag="VFU20_05935"
     CDS             13334..13786
                     /locus_tag="VFU20_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286035.1"
                     /translation="MRYFLDTEFNGFGGALLSLALVPEDGEEFYVTLECADPLFPWVE
                     RNVVPYLDHVPVGLVSPRMSRREAAEALAAYLGSDPEPELVADWPEDVTQFCSLLMIG
                     PGLMVPVPPLTFRLVPLQNFSPAANSAVPHNALHDARSLRDHFCSLLD"
     gene            13814..14476
                     /gene="pdeM"
                     /locus_tag="VFU20_05940"
     CDS             13814..14476
                     /gene="pdeM"
                     /locus_tag="VFU20_05940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ligase-associated DNA damage response
                     endonuclease PdeM"
                     /protein_id="HEU5286036.1"
                     /translation="MVPFSFAGETFVAGPGGALYWPAQKALLVADLHLEKASWFARLG
                     QFLPPYDSHATLTALAREVERSGATRLYCLGDSFHDRFGCDRLPAAARELLLRMTAAL
                     DWVWIVGNHDPGFSDHCGGRLAENLEISGIVLRHEAVRGDPRPEMSGHFHPKLRLNLH
                     GRHVSRRCCVVSATKLILPAFGSLAGGLDATHPAILDNVGSDAAALVPVSDRLLRFPL
                     AA"
     gene            complement(14483..14734)
                     /locus_tag="VFU20_05945"
     CDS             complement(14483..14734)
                     /locus_tag="VFU20_05945"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HEU5286037.1"
                     /translation="MTERSNTADLMETMRFDPDDGILDLDEHLDRLRRSAEALGFEFD
                     RHAARNELQAATFGRKRPAIARLLLSPTGTMAIEVRAAD"
BASE COUNT         2224 a         5252 c         5116 g         2239 t
ORIGIN      
        1 cggcgccgac tgggcggtga tcgacgtcgc gggcgtcggc tatgccgtgc tcgccagcgc
       61 ccgcaccctc gacgcattgg ggccggtcgg cggcgaggta atgttgctga ccgaaatgca
      121 ggtgcgcgag gattcgctaa ccctgttcgg cttcgccacc gccggcgagc gcgaggcgtt
      181 ccgggcgctg acctcggtcc agggcgtcgg cggccgcgtc gctttggcga tcctctcggc
      241 gctcggcccc gacgagctgt cgagcgcggt ggcgtcgggc gacaaggcga tgatcgcccg
      301 agcgcagggc gtcggcccca agctcgccgc acgcatcgcc aacgagctcc agggcaagct
      361 cggagcgccg ggcctggccg gcaccgccgg cgctgccctt ccccgcaccg gcaccgcggc
      421 cgacgcggtc tcggcgctcg gcaacctcgg cttcaagccc gccgaggcca gctcggccgt
      481 cgccgccgcg caggacgagc tcggacccga cgccgagctc gacgcgctgg tccggctggc
      541 gctgaggaaa gcggcgaagt gaggctgccg aaaatagatg cacacacagc ggtgcgagtc
      601 ttgatcgtca ttgcctacgg tctcgctgtc ctcaccacca tgctgcaagc aggggagccg
      661 aacgcgttct ggtggtggct gctgttcttc ccgttctacg cgtggtggtt ggcgccggtc
      721 ggactgccgc tgttctaccc gtggccgtca tggttcatca ctttgccgat tggcgcgatg
      781 gcggcctaca gctcccacat ctacgagagg gacatgttcg gacctggcgc gcgttccacg
      841 tcagccctca ttttcatttt ccttccgatt tatcagtgga tggccgtcgg tgtgctcatt
      901 ggggttgcgt ggctgacccg gcgtctgact aaatgaccac ccgaaccgcc tcctgccgct
      961 gcgggcagct tcgtgtcacc gcaagcagcg agccggtgcg cgtctcagcc tgccattgcc
     1021 ttgactgcaa gaagcgttcg ggcagcgcgt tcgcggtcca ggcgcgctgg ccggcggatc
     1081 gggtcacgat ccagggccgg tcgaaatcct gggtgaatgt cgccgacagc ggcaaccaga
     1141 tcacattcca cttctgtccc gactgcggct ccgacgtcca ttacgagatc aacggcaagt
     1201 tcgatggact gatcgcgatc cccctgggcg cgttcgagga cccctatttt gcctcgcccg
     1261 ctttttccgt ctgggaagag cgcaagcacg agtgggttga gattctcggc gatcaggtcg
     1321 agcatctgga ttgatggcca ccgaccccgc ccgaatcacg tcgcctgagc gcacccccga
     1381 ggacgccgac gcggcgttgc ggccgaagag cctcgacgag ttcatcggcc agcagggcgc
     1441 gcgcgagaat ctccgcgtat tcgtcaacgc ggcgaaaagc cgcggcgagc cgctcgacca
     1501 tgtgctgctg cacgggcccc cggggctcgg caagacgacc ctggccggca tcctcgcccg
     1561 cgaaatgggg gtcggcttcc gcgccacctc cgggccggtg atcgccaagt cgggcgacct
     1621 cgccgcgctg ctgaccaacc tcgaggacgg cgacgttttg ttcatcgacg agatccaccg
     1681 gctccagccg gcgatcgagg aggtgctcta cccggcgatg gaggaccggg cgctcgacct
     1741 gatgatcggc gaggggccgt cggcgcgctc ggtgcggatc gacctgccga gattcaccct
     1801 gatcggcgcg accacccgcc aggggctgct gacgaccccg ctgcgcgatc gcttcgggat
     1861 ccccatccgc ctcaatttct acagcgtgcc cgagctcgag caggtggtgc gccgcgccgc
     1921 ggggctgctc ggggtcgatc tgaaggacga cggcgcgcac gagatcgcgc ggcgctcgcg
     1981 cggcactcca cgggtcgccg ggcggctgct ccgccgggtg cgcgatttcg cccatgcagt
     2041 cggggcgcag gcgatcgatg cgaaggtcgc cgaccaggcc ctggaccggc tcgaaatcga
     2101 cgggctcggc ttggacgcga tggaccgacg ctacctgacc atgatcgccg acatctatgg
     2161 cggcgggccg gtcgggatcg aaaccctcgc cgcgggcctc agcgagccgc gcgacaccat
     2221 cgaggacgtg atcgagccgt tcctcattca gcttggaatc atcgcccgca cggcacgggg
     2281 gcgctgcctc aacggccgcg gctggaagca cctcgggctg accccgcccg cggggagcca
     2341 gagcggcctg ttcgaccagg gagccgcgcc caagtgattc gcgttgcaat gctggtttcg
     2401 ctggccgcct tcgccgcggc ccccgccgcc gcccagccgc cggcgcccgc gcccgattat
     2461 tcgaaacccg cgagctggct gtgcctgccg ggccggacgg atgtctgctc gcagccgctg
     2521 ccgacgacag cgctcaaccc caacggctac ggtcctgtcg ggaacagccg gccgccgccc
     2581 acgccgccgc cggtcgactg cttctacgtc tacccgaccg tgtcgcgcga tgtcggcatg
     2641 aacagcgacc tcgtgcccga cgccagcgag cggtcggcgg cgcaaagcca gttcgcacgc
     2701 ttcgccggcg tttgccggac cttcgccccg ctctaccgcc agctgacggt gagcgcgatc
     2761 gccgcctcgg cggtcggaac cgacctcacc gcgccgtcgg cgctcgccta tcgcgacgtc
     2821 gccgccgcgt ggcgccgcta cctcgccgcg cacaacaagg gccgcccgtt cgtgctcgtc
     2881 ggccacagcc agggctcggc gatgctccag gagctgatcc gccgcgagat cgagggcagg
     2941 ccaatcgcca ggcagatgct gcgggcgatc atccccggct tcaacgtcct cgtgccgcaa
     3001 ggcaggcgcg tcggcggcag cttcaagtcg accccgctgt gcgcatcgcc cagtgagacc
     3061 ggctgcgtga tgagctgggt cagctaccgc gagaacaacg tcccgcccga gggggcgatg
     3121 ttcggctggg cgccgcagcc cgggatgacg gtcggctgca ccaacccggc gcgccccggc
     3181 tcgacgcgct gggagcggct cgacagctat tggaacacgc gcctcagcct gccggtgccc
     3241 ggcgggccga tcgtctggtc gagccagggg tcgccgccga cccagttcct gcgcaccgag
     3301 gggctggtct cggcgcgctg cgtcaatgcc ggccggctcg gctatctgtc ggtccggacc
     3361 aacgccgacc caagcgacaa gcgcaccgac cggatcggcg gcgaggtcgg gatgctcggc
     3421 atgttcatcc ccggctgggg gatgcacctc gcggacttgg cgatcgccca gggcgacctc
     3481 gtccgaaccg tcgaagagct cggccgggcg aagcgctgat cgcccgggtt tgccgcttga
     3541 atccagcccc gctaggggag gcgcgatgac cccctcgccg ttcgaccagc cctaccgcgg
     3601 cggcttcgtc ggccccgagc accatttcgc cctgaccgtc tatttcgagg acaccgacac
     3661 cgccggcatc gtctattacg ccaattatct gaagttcatg gagcgggcgc gatcggacat
     3721 gatccgcgcc gccggggtcg accagaactc ggcccaccat tcgggcggcg gcgcttacgc
     3781 ggtcgccgag gtggacatcc gctatctgaa gcccgcccgg ctcggcgacg acctcgtcgt
     3841 ggtcagcacc gtcgaccagg tccgcgcggt ctcggtgctc attcaccagc gagtcatgcg
     3901 cgggcccgaa ctattgaccg atgcgcgggt caccgcggct ttcctcaccg ccgacggccg
     3961 gccgcagcgc cagcccaagg agtgggtcga gcggttcaag gcgatcggcg aagggcagtg
     4021 atgcggcgag gcggttcgag attggcggtt ctgctcgcgg cgctcggcct tccggccatg
     4081 gcggccgcgc aggacaagcc gccggcggtg atcgccgttc cccagctggc gacgcccgag
     4141 aacgtctcca cgcccgctgg cgataccggg ctgatcgcgc gctatgtcgc cgacgtcatc
     4201 gcgtccgacc tgcgctcgac ccgcgagctg gtcccgatcg gccccgagaa ttcgcgggtc
     4261 tattcctacc cggaatccac ggcgccgtct ttctcgctgt ggcgcaagac cggcgccaag
     4321 acgctggtga ccggcttcgt ccagtcccgc ccggatggcc gaatgaccgt cgcctgctac
     4381 ctccacgacc tggccgcagg gcgcgaaatc gcccgcaagg gcttcgtcgt cgctcccgcc
     4441 gaatggcggc gcgctgcgca tcgctgctcg gacgcggtct atgagcggct cgccggtcgg
     4501 cccggctggt tcgacacccg gatcgcctat gtcgccgaaa gcggcggcgg cgccggccgg
     4561 gtcaagcgga tcgcggtgat ggacagcgac ggcaccgccc accgctacgt gaccgccggc
     4621 gacagcaccg tgctgacccc gcgcctggcg ccgggcggcg agactatcgc ctacgtcagc
     4681 ttcgccgggg gccagccgca catccgcctc gccgacgccg aaggcggcca ggaccggccg
     4741 ctgctgcccg gcactgcgat gagcttcgcg ccgtccttct cggcggacgg caagcggatc
     4801 ctcttttcga tggcgatgga cggcaacacc gacgtctacg tcgccgatgc cgacggcgga
     4861 gcgctacagc ggctgaccag cacgccgggc gtcgacaccg ctccgagctt ctcgcccgac
     4921 ggcagccgga tcgtgttcga aagcgaccgt tcggggagcc agcaattgta tgtgatgaac
     4981 gccgacggct ccgagccgcg ccggatcagc ttcggcggtt cggcctatgc ctcgcccaaa
     5041 tggagcccgg acggcgaatg ggtcgccttc acccggatcg ccggcggcac tctcggcatc
     5101 ggcgtgatga gcccggccgg gaccggcgag cgggtcgtga ccaccggcgc tcacgacgag
     5161 cggccgagct ggtcggccgg cggccgcgac ctgctgttcc agcgcagcga gggcggccgg
     5221 acggggctgt acgcggtctc ggtcgacggc ggagcgccgc ggcggatcgt cactccgcag
     5281 gacggctccg accccgactg gtcgactccg gggagctagc gatgcgccga acgatcctca
     5341 tggtcctggc cctgctcgcc ctgggcggcg cgccgctggc cgcggcgcag gtgatgcgcg
     5401 gcccgcctcc cggcctgctc ccgccgccgg tgaccatcga catgctgcag tcggacctgg
     5461 tcgccaagac cggcagcgac acggtctatt tcgccgggaa cggccacggg atcaacgcgg
     5521 cggggcaggc gacgctctcc gcgcaggccc gctggctgct cgccaacccg gccgtccggg
     5581 cgcggatcga gggccatgcc gacgaccgca cgccgcgcga ctatgcgctg gcggtcggcg
     5641 agcggcgggc cgacgcggtc cgcgatttcc tggtgctgca gggcgttccg gcggcgcagc
     5701 tgacgatcat cagctggggc aaggagcgcc cggcggtcgg cggctcgagc gagatggcgc
     5761 tggccctgaa ccgccgcgcg aggaccgtgc tgatgcccta ggcggcgtgg cgctgctggc
     5821 cggcgccgag ccaggcgcgc gcctggcgct gggcctcggc gatctcgcgg gcggtcatct
     5881 cgatggcgat ctcggcccgg cactgctggc cgcgggtgca gcccgacagg gccgcgagat
     5941 tgaaccattt gtgggcttcg atcaggtcga ccggcgctcc gccgcgtccc gtcgaatagg
     6001 tcaccccgag ctcgaacaat gcctcgacgt cgccgcgctc ggcgtcggca aggcggctgc
     6061 ggatcaggaa ctccgccccc ttctggctga tggcacccat ggcatttccc cctctttgcc
     6121 gttgagtggg ttttcaccgc cgcaatccaa agaaatggtt aacgcggcaa tcacggctgt
     6181 tgcggcgagg caggttcaac cgcgagattg tcgatgaggc gggtgccgcc gatggtcgcg
     6241 gcggcgatca ggcgcatcgc cccgcgcggc tcgtcgatgg gctcgagcgt ggcggcgtcg
     6301 accagcgcga cataatcgac ggtcgagaaa ccggcgttac ggagcgcctg cctggccgcc
     6361 gacagcgccg cctcggccgt ctcgccggac aggatcgcgt cccgggcggc cgcgagggcc
     6421 tgcggcaagg cgagcgcccg cttgcgctcg tcggcggaga gataggcgtt gcgcgacgag
     6481 agggcgaggc cgtccgggtc gcggaccgtc gggaccccgg cgatctcaac cccgaggccg
     6541 aggtcggcga ccatgcgccg gatcaccgcg agctgctgaa aatccttttc gccgaacagg
     6601 gcgacatcgg gccgaaccgc aagcagcagc ctggcgacga ccgtggcgac gccgtcgaaa
     6661 tggccggggc gggcctcgcc ctcccagcgg tcgccgagcc cgctcacgct caccttggtc
     6721 gcgaacccct ccgggtagat gtcgccgacc gagggaatcc acagcaggtc gcagccggcc
     6781 tgctcgagca tctccgcgtc cgcggcttcc cggcgcggat agcggtcgag gtcctcgttg
     6841 gcgccgaact gcatcgggtt gacgaagatc gtcgccacca cccggtccgc cagccgcttc
     6901 gcctcggcca ccagggccat gtggccggcg tgcagcgcgc ccatggtcgg aaccagcgcg
     6961 atgctcgcgg tccgattatc gcgcggcgcg ctccggagcg cgaaaacttc accgggcaag
     7021 gctcctatct cacggattat ttgcaaggat tccgcccctc cgatacactt tgcgccgacg
     7081 gcgctttgcg gcgcgccggg gggaaagatc aagcgacatg agcgacccgc atttcatcat
     7141 cttcgccaac gagaagggcg gcaccggcaa gtcgaccacc gccgtccaca cggcgatcgc
     7201 tctcgccgcc tcgggccacc gcgtcgcggc gctcgacctc gacgcgcgcc agcggaccat
     7261 ggcccgctat ctcgagaacc gcgacgcgac catgcgccgg ctcgacaagc agctgcccca
     7321 cgcgcgcttc gaagtgctgg acgatcacac cgaaacaggc ctggccgccg cggtcgcgcg
     7381 cctgtccgag gacgtcgacg tgatcgtcgt cgacacgccc ggccgcgatg acccggtcag
     7441 ccgcgccgcg atcctcgagg cggacactct ggtgacgccg atgaacgaca gcttcgtcga
     7501 cctcgacctg atcggccagg tccacccgga aaattacaag atcaccaagc ccagcttcta
     7561 cgccgagctg atctggaaca gccgcaccca gcgcgccaag gcgaccgggc ggagcgtcga
     7621 ctgggtggtg ttgcgcaacc gcctgcagca cgtcgacagc cacaatttgc gccgcgtcgg
     7681 ggcggcgctc gacgagctgg cgcgccgcgt cggcttccga gtcatcccgg gcctcggcga
     7741 gcgcgtcatc taccgcgaat tgttccccaa gggcctgacc ctgctcgact tcgagcatct
     7801 cggcgaagtc ggcctcgggc acatcaccgc ccgccaggag ctgcgcgaga tgatcgccgg
     7861 gctcggcatc cccgagccgg gcgccagcgt gcccgagccg gtcgccgccg ccggctgagc
     7921 ctttccaggc ctttttgtag ttatgacgac atgagccacc gattccacgc gtccgtcctg
     7981 cgcgaatatg acgtccgcgg gatcgtcggc gacactttga ccgaggccga cgctcttgcg
     8041 ctcgggcgca gctatgcggc gctggcgaag gaggaaggcg cggcccgggt cgccgtcggg
     8101 cgcgatgggc gcacccattc gcccgggctg gaggcggcac tcgtcgacgg cctcacccaa
     8161 agcggtctcg acgtcgtccg catcggcatg gggccgagcc cgatgctcta cttcgccacc
     8221 gcgacattgg gcgtcgacgg cggcatccag gtcaccggca gccacaaccc ggccgactac
     8281 aacggcttca agctgctcct gaagggccgc tcggtgttcg gcgaggagat ccaggcgctc
     8341 ggccgccgcg ccgcggccgg cgactggaac gagggctcgg gcacagtcga tgacgccgac
     8401 gtgctcgact cctatgccga ccggctggtc gcggacttcg ccggcggccc gttccgagtc
     8461 ggctgggacg ccggcaacgg cgcggcgggt ccggtgctgg agaaattgat cgagcggctt
     8521 ccgggcgagc accacacgct cttcaccgcg gtcgacggca ccttccccaa ccaccacccc
     8581 gacccgaccg tcgaggccaa cctcgccgac ctgaaggcgc tggtcgcggc caagggcctc
     8641 gacttcggga tcgccttcga tggcgacggc gaccgcatcg gcgcggtcga cggcaagggc
     8701 cgggtgatct ggggcgacca attgctggcg atcctcgccg ggccggtgct cgccgaatgc
     8761 ccgggcgcga ccatcatcgc cgacgtcaag gcgagccagc tgctgttcga ccggatcgcc
     8821 gagctcggcg gacggccctt gatgtggaag accggccaca gcctgatcaa ggcgaagatg
     8881 aaggagaccg gcgcgccctt ggccggcgag atgagcggcc acatcttctt tgcccaccgc
     8941 tggtacggct tcgacgacgc gctctacgcc ggcgtgcggc tgatcgaggc ggtcgcggaa
     9001 tcgggcaaga gcctaaccga gctgatggat gcgatgccga ggtcggtggc gacccctgag
     9061 ctccgcttcc cggtcgaggc gagccgcaag ttcgcggtga tcgacgaggt gctcgagcgc
     9121 ctctcagccg aaggtgcaat agtcgacggc accgatggcg cgcgcgtgag caccgaggac
     9181 ggctggtggc tgctccgcgc atccaacacc caggacgtcc tcgtcgcccg cgccgaagcg
     9241 aaggacgaag cggggctagc gcgcctcgtc gcgcagatcg acaagcaatt ggcgaagtcg
     9301 ggagtcatgc ggaccgaagt cgcgcactga cgaattcgct caatcatcct cgtcctcata
     9361 gccgactaaa ttcagctccc gcgccttgat ctggtgggtc atgcaccagt gcatcagcgc
     9421 atcctcgcgg ccatgcgtga tccacacttc cctgggcgcg acgtcgcgga tggtctgggt
     9481 gagctcgtcc cagtcggcgt ggtcggagat caccagcggc agctcgatgt tgcgctggcg
     9541 ggcgcgctgc cggatccgca tccagcccga cgccatcgcc gtcaccgggt cgggcagccg
     9601 ccgcgaccag cggtcgttga gcgcgcccgg cgggcagagg acgatgtgcc cggcgatctc
     9661 ctgcttggtc gcgccggccg tatggcgcag ctcgccgagc tcgaccccga actgctcgta
     9721 gaggcggcag agccgctcga gagcgccgtg gtagtagatc ggctcgcggt ggccgcgggc
     9781 gcgaagctcg gcgatcaccc gttgcgcctt gccgagcgca taagcgccga ccagcacgca
     9841 gcgcgacggg tccgagtgca gccggtggag cagccggtcg atctcgctgg ccgtgtcggg
     9901 atggcggaac accggcagtc cgaaggtcgc ctcggtgatg aagatgtcgc acggcaccgg
     9961 cacgaacggg gcgcaggtcg ggtcgtcgcg gcgcttgtag tcgcccgaca cgacgacgcg
    10021 ctcgccggca tgctcgagga cgatctgggc cgagccgagc acgtggccgg cggggacgaa
    10081 acggatgtcg acctcgccga ggcggaccgt ttcgtcatag gcaatgccct gccccggctg
    10141 cggtccgtag cgcacggcca tgatcgccag cgtttcgggc gtcgcccaca cctcgtcgtg
    10201 gccgccgcgc gcatggtcgc cgtggccgtg ggtgatcagc gcccgcgcct tgggctcgga
    10261 ggggtcgacc caggcgtcgg ccgggcgcac atagatgccc tcgggagaag gctcgatcca
    10321 ggaacccaag cgggccatcg cgagctatag tgagcgaggc agccgggcgg ttcccggtgg
    10381 aaagcgaacg gccgggggac ccggcatgga gacgatgaat ggacgaaacc ctgggcagcc
    10441 tgatgaccat catcggcccg atcatcctgc tggtcctgct ggtctggctg gtgatgcgat
    10501 cgcggcgccg gagcggccgg ccgaccgaca ccaccgccca gaccgagcaa gcgaccgacc
    10561 ggctgtacga cgacgaggag cggcgccgcc gcgaagggac cgacgacctc tgagcgcgag
    10621 cgcgctgccg gagattgtcc gccgctggtt cgagggccgc ggctgggaac cccggcgcca
    10681 ccagctcgca atgctcgacc tcgcgcgggc caggcgaagc gggctgctgg tcgccgcgac
    10741 cggggccggc aagaccctag ccgggttcct tccgaccatc tgcgacctga tcgagcatcc
    10801 ggccgagggc ctgcacacgc tctacgtgtc gccgctgaag gcgctgggcg tcgacgtgca
    10861 gcgcaacctg ctcggaccga tcggcgagat ggggctggcg atccgggtcg agacccgcag
    10921 cggcgacact ccgtccgacc gcaaggcgcg gcagcgggtg cggccgccgc acatcctgct
    10981 gacgaccccc gaatcgctga gcctgctgct gagctatccc gagagcgcga ccctgttcgc
    11041 gggcttgaag accatcgtcg tcgacgagct gcacgcgttc gccaaggaaa agcgcggcga
    11101 cctgctggcg ctggcgatgg cgcggctgca gaccttcgcg ttggggctta ggcgggtcgg
    11161 gctgtcggcg acgatcagcg atcccgacgc gtaccgcggc tggctggcgc ccgacgcgga
    11221 cgagacccag gtcgacctgg tcaccggcga ccccggcgcc gagcccgacc tgtcgatcct
    11281 gatccccgac gggaagatcc cgtggtcggg gcactcgggc cgccacgcgg cgcgcgaggt
    11341 gatggagctg atcgagcggc acaagatgac gctgatcttc tgcaacacgc gcagccttgc
    11401 cgagctgatc ttccaggatt tgtgggcggt caacgattcg gccctgccga tcggcatcca
    11461 ccacggcagc ctggccctgg aggcgcggcg caaggtcgag gcggcgatga gctcggggaa
    11521 attgcgcggg ctggtcgcga ccgccagcct cgacctcggg atcgactggg gcgacatcga
    11581 cctcgtcgtc cagatgggcg cgcccaaggg gagctcgcgg ctgctgcagc ggatcggccg
    11641 cgccaaccac cggctcgacg agccgagcaa gggcatcatc gtgcccggca accgcttcga
    11701 atatctcgag gcgcgcgcgg cgctcgacgc gatcgatgac ggcgagctcg accccgagaa
    11761 tttccgtgcc ggagccctcg acgtgctcgc ccagcacatc ttggcgctcg cctgctcggc
    11821 gccgttcgag gaggccgagc tgctcgccga gttgcgctcg gccgcgcccc acgcaggcct
    11881 tcgcgacgag accttcacgg aagtcctcaa tttcatcgcc accggcggct atgcgctcaa
    11941 ggcctatgac cgcttccgcc gcctggtgcg cgagcccgag ggccgctggc ggatcgcgcg
    12001 gcctgggatc gccgcccagc accggctcaa cgcgggcgtg atcgtcgagc agccgctgct
    12061 gacggtccgc ttccgcggcg gccgcaagct cggcaccatc gaggaaggct acgcctcgac
    12121 cctggcgccc ggcgaccatt tctattttgc cggcctcagc ctcgaggtcg agcagttcaa
    12181 ggacaccgac atcattgtcc atgcgtcggc caagacggcg cggatcgtca cctacggcgg
    12241 ccagcgcatg tcgatgtcga cccatctcgc caaccgcgtc cggagcatgc tcgccgaccg
    12301 caacgactgg caccgcttcc ccgacgacgt ccgcgaatgg ctcgaggtcc aggaccgccg
    12361 ctcgacgctg cccgagcccg agcaattgct ggtcgagacc ttcgagcacc acggccagca
    12421 ttacctggtc gcctacagct tcgagggctg gaacgcgcac cagtcgctgg gcatgctgat
    12481 caccaagcgg atggagaata tgggtttgaa gcccttgggc ttcgtcgcca acgactacgg
    12541 cctcgcctgc tacgggctcg agccgatccg cgaccccaag gccctgttct cgcccgacat
    12601 cctcgagcag gagttcgtcg actgggtcga gcaatcctat ctcctgaaga gcgcattccg
    12661 cgaggtggcg gtgatcggcg ggctggtcga gcgccaccac ccgggcaagc gcaagtcggg
    12721 ccggcaggtg agcttctcga ccgacctcat ctacgacgtc ctgcgccgct acgagcccca
    12781 gcatctgctg ctgcgcgcgg cgtgggacga tgcccgcgcg cggatgaccg agctcggccg
    12841 gctggtccgc ctggtcgacc gcgcgtcggc gaccatggtc cacgtcgagg ccgaccggat
    12901 cacgccgatg gcggtgccgc tgatggtgat cgtcgggcgc gaggcgatgc cgcccggcgg
    12961 cgaggccgac gagacgcttc tgatccaggc cgaatcgctc gcccgggaag caatggcggt
    13021 ctgacgctcg ttcattgctt gtgaagcaag catctgcgag tctggaacct atgaaagccc
    13081 tcgtcctcac cgccgcggca accgcgctga gcggttgcgt cgtcgcggac gttgccgaga
    13141 cggcggtcgg tgtcgccaag atacccgtga aggtcgcctc ggccggggtc gatgcggcga
    13201 ccaccagcca gtcagaggcc gacgagaagc gcggccgcga gctgcgcaaa gccgacgaga
    13261 agcgcgcccg cgaggagcgc gaggcggcaa aaaaggctgc ggagcgcccg caaagcgagt
    13321 agcgcgccta cccgtgcgct atttcctcga caccgagttc aacggcttcg gcggagcgct
    13381 gctgagcctc gccctggtgc ccgaggacgg cgaggaattc tatgtgaccc tggaatgcgc
    13441 ggacccgctg ttcccgtggg tcgagcgcaa cgtcgtccct tatctcgacc atgtgccggt
    13501 cgggctggtg tcgccgcgga tgagccgccg cgaggccgcc gaggcgctcg ccgcctacct
    13561 cggttccgat ccggagcccg agctggtcgc cgactggccc gaggatgtga cccagttctg
    13621 ctcgctgctg atgatcgggc ccgggctgat ggttccggtg ccgccgctga ccttccggct
    13681 ggtgccgctg cagaatttca gcccggccgc caacagcgcc gtgccccaca atgcgctgca
    13741 cgacgcgcga tcgcttcgcg accatttctg cagcctcttg gattaaccga ccagatcggg
    13801 tttaagcggg gcgatggttc ccttttcgtt cgcaggtgaa acgttcgtcg ccgggcccgg
    13861 cggcgcgctc tactggccgg cccagaaggc gcttctggtc gccgacctgc acctcgagaa
    13921 agcgagctgg ttcgcgcggc tcgggcagtt cctgccgccc tacgattcgc acgcgacctt
    13981 gaccgctctg gcgcgcgagg tcgagcgcag cggagcgacg cggctctact gcctcggcga
    14041 cagcttccac gaccgcttcg gctgcgaccg cctgccggcc gccgcgcgcg agctgctgct
    14101 gcgaatgacc gcggcgctcg actgggtgtg gatcgtcggc aaccacgatc cgggcttttc
    14161 cgaccattgc ggcggccgcc tcgccgagaa tctcgagatt tccggcatcg tcctccggca
    14221 cgaggcggtt cgcggcgatc cgcgccccga gatgtcgggc catttccacc cgaagttgcg
    14281 gctgaatctc cacgggcggc acgtgtcgcg gcgctgctgc gtggtttcgg cgaccaagct
    14341 gatcctgccg gcgttcggtt cgctggccgg cggcctcgac gcgacccatc cggcgatcct
    14401 agacaatgtc ggcagcgatg cggcggcgct ggtgccggtg tccgaccggc tgctgcgctt
    14461 ccccctcgcc gcctgagcgc ggtcagtccg cggcccggac ctcgatcgcc atggtgccgg
    14521 tcggcgagag cagcagccgg gcgatcgccg gccgcttgcg cccgaaggtc gccgcctgga
    14581 gctcgttgcg cgccgcgtgg cggtcgaact cgaagccgag cgcctcggcc gagcgccgca
    14641 gccggtcgag atgctcgtcc agatcgagga ttccgtcgtc ggggtcgaag cgcatggtct
    14701 ccatgaggtc ggcggtgttg ctgcgttcgg tcatcgggcc cttaatcggc gtcggaaccg
    14761 gcgagggcaa gcctgcggcg attgcgcggc cgcgtccctc ctcttagacg gtcgcgcatg
    14821 aaaactttct c
//
LOCUS       DASTZO010000212         4154 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1140936,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000212 DASTZO010000000
VERSION     DASTZO010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140936"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(251..991)
                     /gene="truA"
                     /locus_tag="VFU20_05950"
     CDS             complement(251..991)
                     /gene="truA"
                     /locus_tag="VFU20_05950"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU5286038.1"
                     /translation="MTRWRLTIEYDGRPFMGWQRQDNGPSVQQQVEAAIFRMTGELAS
                     VHAAGRTDSGVHALAMSAHADITKTLTAHRLRDGLNALVRPDPIAILAASEVADDWHA
                     RFSCIGRRYRYRILNRRAPPAIERGRVWHVAVPLDGGAMAEGAAMLVGRHDFTTFRSA
                     QCQSQSPVKTLDRLDVTRDGDEIRVEAAARSFLHHQVRSMVGCLALVGRGQWTPADIR
                     AALDARDRAALGLNAPPEGLYFVEALYP"
     gene            complement(988..1887)
                     /gene="fmt"
                     /locus_tag="VFU20_05955"
     CDS             complement(988..1887)
                     /gene="fmt"
                     /locus_tag="VFU20_05955"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU5286039.1"
                     /translation="MRVIFMGSPEFAVPSLDTLVGAGHEIAAAYCRPPRPAGRGKAER
                     KTAVQRRAEALGIEVRTPASLRSAQEQARFRSLDADLAVVAAYGLILPAPILAAPRAG
                     CINVHASLLPRWRGAAPIQRAILAGDAVSGVTIMRMDEGLDTGPMLLRRELPIAGKNA
                     GEVTQELAQLGAEALLEWLAKPTPAVPQPEDGVTYAAKVAKAEARIDWSRGAEEVVRQ
                     VLAFAPAPGAWFEAGGERVKLLDAELGNGSGKPGEVLDERLAVACGQGAVRPLVIQRA
                     GRAPMTPQELLRGFAIPAGTVLE"
     gene            1996..2592
                     /gene="recR"
                     /locus_tag="VFU20_05960"
     CDS             1996..2592
                     /gene="recR"
                     /locus_tag="VFU20_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU5286040.1"
                     /translation="MASQEIEALSQALARLPGLGPRSARRAVLHLMKRRETALEPLLA
                     ALRDVATRLSTCSTCGNLDTSDPCGICSDPRRDDKLLCVVEEVADLWALDRSRLFPGR
                     YHVLGGRLSALEGVRPEDLGIDKLVARVAAGGIDEVVLAMNATLEGQTTAHYLAERLE
                     KYPVRLTQLAHGLPVGGELDYLDEGTLAQALRARRPIA"
     gene            2624..3163
                     /gene="def"
                     /locus_tag="VFU20_05965"
     CDS             2624..3163
                     /gene="def"
                     /locus_tag="VFU20_05965"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU5286041.1"
                     /translation="MAILPIVEVPDPRLRQISSPVETVDGEVRKLIADMFETMYAAPG
                     IGLAAIQVGVPKRVLVMDLQEPEEEGGEPVRDPRVFINPEILTRSDQEVPYLEGCLSV
                     PDQYADVERPDRIRARWLDEHGKPHEADLEGLIAVCLQHEMDHLEGILFIDHLSRLKR
                     EMVLKKLAKMRKERALRAA"
     gene            complement(3293..3685)
                     /locus_tag="VFU20_05970"
     CDS             complement(3293..3685)
                     /locus_tag="VFU20_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006950732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-helix bundle copper-binding protein"
                     /protein_id="HEU5286042.1"
                     /translation="MSIRKMIALHPAAKEHVNQPLGDAVHHAMYCAKMCASCADACLA
                     EAMDMTQCIRSCLDCADVCEAAAKLGLRRSASNEAVLKEMLELCARTCDACAAECDRL
                     DHEHCKLCAQICRECADDCRAAALTISS"
     gene            3812..>4154
                     /locus_tag="VFU20_05975"
     CDS             3812..>4154
                     /locus_tag="VFU20_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU5286043.1"
                     /translation="MDLSVILIALIALALGALIGWLVGSRASASSREVIDNLRLQLDA
                     VAQERDSNRSAASELAVLQAAQEERERSFQQQLEAVREAKESLSAQFHEIGGKLLGEA
                     RKAFLEQAGERF"
BASE COUNT          613 a         1445 c         1450 g          646 t
ORIGIN      
        1 cgttccagat gtcgacacgc tcgtacggca ggatgtccgc cgcttcgagc agattggcgt
       61 cgatggtcac cgagccctcg tagtcgaggt cggcctgggt caccgtcgcc cggtggatct
      121 tggatttcat catcacccgc atcacggggt gggttccttt tcgaacggcg gccgcgccac
      181 tagcagattg gccgcgccac tagcagattt ggtctcgctc gccatccttc aagtgctgca
      241 gtgcacaatc ctaggggtag agcgcttcca cgaaatacag cccttccggc ggcgcgttga
      301 gccccagcgc ggcgcggtcg cgggcgtcga gcgccgccct tatgtcggcc ggcgtccatt
      361 gtccgcgccc gaccagcgcc aggcagccga ccatcgagcg cacctggtgg tggaggaagc
      421 tgcgcgccgc cgcctcgacc cggatctcgt cgccgtcgcg ggtgacgtcg agccggtcga
      481 gcgtcttgac cgggctttgc gactggcact gggccgagcg gaaggtggtg aagtcgtggc
      541 ggccgaccag catcgccgcg ccctcggcca tcgccccgcc gtcgagcggc acggcgacgt
      601 gccacacccg gccgcgctcg atcgccggcg gagcgcgccg attgaggatc cggtagcggt
      661 agcgccgccc gatgcacgag aagcgcgcgt gccagtcgtc ggcgacttcg cttgcggcga
      721 ggattgcgat cgggtcgggc cggaccagcg cgttcagccc gtcgcgaagc cggtgcgcgg
      781 tcagcgtctt ggtgatgtcg gcatgcgcgc tcatcgccag cgcatggact ccggaatcgg
      841 tgcggccggc ggcgtggacg ctcgccagct cgccggtcat ccggaagatc gccgcctcga
      901 cctgctgctg gaccgagggc ccgttgtcct gccgctgcca gcccatgaac gggcggccgt
      961 catattcgat ggtcagccgc cagcgcgtca ttcgaggacc gttccggcgg gaatggcgaa
     1021 gccgcgcagc aactcctgcg gcgtcatcgg cgcccgcccg gcgcgctgga tcaccagtgg
     1081 ccggaccgcg ccctgcccgc aggcgaccgc cagccgctcg tcgagcactt cgccgggctt
     1141 gccggagcca ttgccgagtt ccgcatcgag cagcttgacc cgctcgccgc cggcctcgaa
     1201 ccaggcgccc ggggccgggg cgaaggccag cacctgacgg acgacctctt cggcgccgcg
     1261 gctccagtcg atccgggcct cggccttggc gaccttggcc gcgtaggtga cgccgtcctc
     1321 gggctggggc accgccgggg tcggcttggc gagccattcc agcaaggctt ccgcgcccaa
     1381 ttgtgcaagc tcttgcgtga cttccccggc gttctttccg gcgatcggaa gctcgcgcct
     1441 tagcagcatc gggccggtgt cgagcccttc gtccatgcgc atgatggtca ccccgctgac
     1501 cgcatcgccg gcgaggatcg cgcgctggat cggcgccgcg ccgcgccacc gcggcagcag
     1561 cgatgcgtgg acgttgatgc agccggcgcg cggcgccgcg aggatcggcg cgggcaggat
     1621 cagcccgtag gccgcgacca ccgccagatc ggcgtcgagc gaccggaagc gcgcctgctc
     1681 ctgggcggac ctgaggctgg ccggcgtgcg gacctcgatg ccgagcgcct ccgcgcgccg
     1741 ctggacggcg gtcttgcgct ccgccttgcc gcgcccggcg ggccggggcg gccggcagta
     1801 agcggcggca atctcgtgcc cggccccaac caaggtgtcg aggctcggca ccgcgaactc
     1861 ggggctgccc atgaagatta cacgcaaaga aaatcccgtt cgcccagagc ctgtcgaagg
     1921 gctgtcctta ccatctcagg cggaaagtga agaacagggc ttcgacacgc tcagcccgaa
     1981 cggtatgggg tcaatatggc atcgcaggag atcgaggcgc tttcgcaggc gctggcgcgg
     2041 cttccggggc tcgggccgcg ctcggcgcgc cgcgcggtgc tgcacctgat gaagcgccgc
     2101 gagaccgcgc tcgagccgct gctggccgcg ctccgggacg tcgcgacccg gctgtcgact
     2161 tgctcgacct gcggcaacct cgacacgtcg gacccgtgcg gcatttgctc ggacccgcgc
     2221 cgcgacgaca aattgctgtg cgtggtcgag gaggtcgccg acctgtgggc gctcgaccgc
     2281 tcgcggctgt tcccgggccg ctatcacgtg ctcggcgggc gcctgtcggc gctcgagggc
     2341 gtgcggcccg aggaccttgg catcgacaag ctggtcgcac gcgtcgccgc gggcggcatc
     2401 gacgaggtgg tgctggcgat gaacgcgacc ctcgagggtc agaccaccgc ccattatctc
     2461 gcggaaaggc ttgagaaata tccggtccgg ctgacccagc tcgcgcacgg cctgccggtc
     2521 ggcggcgagc ttgattatct cgacgagggg acgcttgccc aggcgttgcg cgcaaggcgg
     2581 ccgattgctt gaagcaagcg cctccagcga ctaaataacc gccatggcca tcctccccat
     2641 cgtcgaggtc cccgacccgc gcctgcgtca gatctcctcg cccgtcgaga cggtcgacgg
     2701 tgaggtgcgc aagctgatcg ccgacatgtt cgagacgatg tatgcggcgc ccggcatcgg
     2761 cctcgccgcg atccaggtcg gagtccccaa gcgggtgctg gtgatggacc tccaggagcc
     2821 cgaggaggag ggcggcgagc cggtcaggga cccgcgggtg ttcatcaacc cggagatcct
     2881 cacccgctcc gaccaggaag tgccctatct cgagggctgc ctgtcggtgc ccgaccaata
     2941 tgccgacgtc gagcggcccg atcgaatccg cgcccgctgg ctcgacgagc acggcaagcc
     3001 ccacgaggcc gacctcgagg ggctgatcgc ggtctgcctg cagcacgaga tggaccatct
     3061 cgagggcatt ttgttcatcg accatctgtc gcggctcaag cgcgagatgg tgctcaagaa
     3121 gctcgccaag atgcgcaagg agcgcgcgct cagggccgcc tgatctcatt cctccccgga
     3181 acggggaggt ggcaggcgcg gagcgactga cggagggggc ccagcgaccc gcaccacgcc
     3241 ccccaccgtc gaatcttcgt tcgctggtcc cactccccgt tccggggagg acctaggagc
     3301 tgatcgtcag cgccgcggcc cggcagtcgt cggcgcactc gcggcagatc tgggcgcaca
     3361 gcttgcaatg ctcgtggtcg agccggtcgc actcggcggc gcaggcgtcg caggtccgcg
     3421 cgcacagctc gagcatctcc ttcagcaccg cctcgttgga ggcgctccgg cgcaggccca
     3481 gcttcgccgc cgcctcgcac acgtcggcgc agtcgaggca cgagcggatg cactgggtca
     3541 tgtccatggc ttccgcgagg caggcgtcgg cgcacgacgc gcacatcttc gcgcaataca
     3601 tcgcgtggtg gacggcatcg ccgagcggct ggttgacgtg ctccttcgcg gccggatgga
     3661 gcgcgatcat cttgcggatg gacatggact tccccttcgt gccggcaagc actgaaccgg
     3721 tgcggtcggt caaggttccg cttgagcggc ccgcccgccg gccctagagt tcacactctg
     3781 ttcccgccca agggacgagg ggagtcggtc gatggacctg agcgtcattc tcattgccct
     3841 gatcgcgctg gcgctgggcg ctctgatcgg ctggctggtc ggcagccgcg cctccgcgtc
     3901 gagcagggaa gtgatcgaca atttgcgcct gcagctcgac gcggtcgccc aggagcgcga
     3961 ttcgaaccgc tcggcggcca gcgagctggc cgtgctccag gccgcccagg aggagcgcga
     4021 gcgttcgttc cagcagcagc tggaggcggt gcgcgaggcc aaggagagcc tgtcggcgca
     4081 attccacgaa atcggcggca aattattggg cgaggcgcgc aaggcgttcc tcgagcaggc
     4141 cggcgagcgc ttca
//
LOCUS       DASTZO010000213         6419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_850026,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000213 DASTZO010000000
VERSION     DASTZO010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6419
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850026"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..778
                     /locus_tag="VFU20_05980"
     CDS             356..778
                     /locus_tag="VFU20_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010411205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5286044.1"
                     /translation="MTLPPFHFAFPVDDLAAARDFYGRVLGCPEGRSADHWVDFDLRG
                     HQIVAHLAPGAARNRASNPVDGEAVPVPHFGLVLAMDEWRALAERLEAAGVEFIIPPT
                     VRFAGEPGEQATMFLADPAGNALEFKAMADPGKLFAAK"
     gene            complement(794..1669)
                     /locus_tag="VFU20_05985"
     CDS             complement(794..1669)
                     /locus_tag="VFU20_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU5286045.1"
                     /translation="MATQQDEVGSRLWRGMRALIFGDDGETSLRDQIEEAIDEAETAR
                     PVTGDLSPHERQMLRNLLQMGDRTAGDICVTRGDIIAVPATTGFEDLIRAFADAGHSR
                     LPVYGDSLDEVVGMYHIKDVFIADVDETRDRSPGSLMRTPLFVPESMGVIDLLARMRA
                     DHIHLAIVVDEFGGTEGLVTIEDVVEEIVGEIEDEHDEETGRMLILLEEGLWEADARL
                     ELEELTATVDARLTAEEDEIDTLGGLVFLIAGRILKPGESVVHPSGWRLECVDADGRR
                     IRRVRLHAPEVPGAA"
     gene            complement(1669..2175)
                     /gene="ybeY"
                     /locus_tag="VFU20_05990"
     CDS             complement(1669..2175)
                     /gene="ybeY"
                     /locus_tag="VFU20_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU5286046.1"
                     /translation="MMLEVAIETDAEWDSSTDWQALARAAAESAIAESAFPHLATGPR
                     PVELSVRLTGDGQVQALNEQWRGKDRPTNVLSFTMAEPGELEAASGDGPELMLGDVVL
                     AHGVCAAEAEAKAVPIADHAAHLLVHGTLHLLGCDHQDDRSAAAMEQREVRALARLGI
                     ADPYEGTR"
     gene            complement(2183..3175)
                     /locus_tag="VFU20_05995"
     CDS             complement(2183..3175)
                     /locus_tag="VFU20_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU5286047.1"
                     /translation="MARRDLAAVPDRARLELEFDQPYLLGPLFGDYDRHLIAIEQRLG
                     VHIAARGNRVQIEGEADAAARARDVLVGLYNRLDQGHDVDAAAVESVIAMAAQPSLDG
                     IIEDEVTSPPRVMIRTRKKTVVPRSTVQTAYMEALARDDMIFALGPAGTGKTYLAVAQ
                     AVSQLISGSVDRLILSRPAVEAGERLGFRPGDMKDKVDPYLRPLYDALYDMLPTEQVE
                     RRIASGEIEIAPLAFMRGRTLNDAFIILDEAQNTTPAQMKMFLTRFGMRSRMVICGDP
                     TQVDLPDPGKSGLADAVSRLSGIKSLAVIRFTAADVVRHPLVGRIVEAYEGPGA"
     gene            complement(3175..4500)
                     /gene="miaB"
                     /locus_tag="VFU20_06000"
     CDS             complement(3175..4500)
                     /gene="miaB"
                     /locus_tag="VFU20_06000"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEU5286048.1"
                     /translation="MTSPLKSFRIKSFGCQMNVYDGERMAELLGTTGLAPAADGEEPD
                     LVVLNTCHIREKAAEKAYSDIGRLRREDGSRPLIALAGCVAQAEGAEAAARSKMIDIV
                     VGPQAYHRLPEMVAQAARGDRPVDTAMPALSKFDALPRRRRAAPSAFLTVQEGCDKFC
                     TYCVVPYTRGAEISRPWGDIVAEARALVEGGAREIVLLGQNVNAWNDDGRGLDALIRA
                     LSGIDGLERIRYTTSHPADMSDGLIAAHGEVGKLMPYLHLPVQSGSDRILKAMNRSHS
                     TDSYLRTIDKVRAARPDIAFSGDFIVGFPGETDADFEATLSIVDAVGYAAAYSFKYSA
                     RPGTPAAAMDGQVALQVMDERLQRLQARIAEHQLAFNRASVGRRTSVLVERRGRKGGQ
                     MIGRSPWLQSVHFESAAEPGDMVDVTLVAAGPNSMAGAAPAAQNARAAA"
     gene            complement(4513..5229)
                     /locus_tag="VFU20_06005"
     CDS             complement(4513..5229)
                     /locus_tag="VFU20_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU5286049.1"
                     /translation="MRIAALGGWFLLCVVPHLVSKLFGRSRWPRRFLSGSAAICGVDV
                     QVEGKLANSQSMLLANHTSWLDVPVLAAATGCAFVSKAELSRHPVMKWLADQNRTLYI
                     DRSDRRGLHDQTRAVRRALTGDQPLALFPEATVANGGRLLPFRAALLTAVAPPPPGCA
                     IQPAAIDYGPATPALGWARGEHGLANAVRILGRRGRMTATVRLLEPLDPSLDRKAMAK
                     AAHDAIAAALAPSGIAPAGL"
     gene            complement(5258..5680)
                     /locus_tag="VFU20_06010"
     CDS             complement(5258..5680)
                     /locus_tag="VFU20_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HEU5286050.1"
                     /translation="MHRDIDIEALCADKGLRITEQRRTIARILSEAEDHPDVETLHER
                     AAAIDPKISIATVYRTVRLFEEAGILERHEFGDGRSRYEAASDAHHDHLIDVETGNVI
                     EFVDEDLEELQKRIAEKLGFRLVDHRMELYGVALDRGR"
     gene            complement(5747..6172)
                     /locus_tag="VFU20_06015"
     CDS             complement(5747..6172)
                     /locus_tag="VFU20_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HEU5286051.1"
                     /translation="MVDNEGAEDTLLALTADIVAAHVSNNSVAVNDLPNLIQNVHAAL
                     STISDTRSAPEARPEPKVPIRSSVKPDYIVCLEDGKRLKMLKRHLMTHYQMTPDQYRQ
                     KWGLNADYPMVAPNYAEQRRALAKSIGLGTKRRKTRGAK"
BASE COUNT          937 a         2190 c         2255 g         1037 t
ORIGIN      
        1 agtccggcaa cgccttcatc agcatcaacg atcgcgaccg cgaagtgctg ggctatgcgc
       61 gggttaccct tccgctcggc ggcaagccga agcggatcga ctgctcgaag gtctacgagc
      121 tcgagatcga acggctgaag caggaaatcg agctaatgaa gatgaacgcg gaatagtcgg
      181 cgcacctggc gacaggggcg acgcccgtcc tgaagccctc tgccatcgaa aatcaaaagt
      241 ccgcttcggg tcggaagcag gcgccgggcg ggattgggac cgtcagccga tgcgggctgc
      301 acgccttgat cgcttcccct tgctttcgcg ctgctcgcga tgacaaagcg accttatgac
      361 cctgccgccg ttccacttcg ccttcccggt cgacgacctc gccgcggcgc gcgacttcta
      421 cgggcgcgtg ctcggctgcc cggaggggcg cagcgccgac cattgggtcg atttcgacct
      481 tcgcggccac cagatcgtcg cccatctcgc gccgggcgcg gcgcgcaacc gggcgagcaa
      541 cccggtcgac ggcgaggccg tgccggtgcc gcatttcggg ctggtgctgg cgatggacga
      601 gtggcgcgcg ctcgccgagc gtctggaggc ggcgggggtc gagttcatca tcccgccgac
      661 cgtccgcttc gccggcgagc cgggcgagca ggcgaccatg ttcctcgccg acccggccgg
      721 caatgcgctc gagttcaagg caatggccga tcccggcaaa ttgttcgccg cgaagtaacc
      781 aggaaaacgc agatcaggcc gcgcccggga cctcgggggc gtggagccgg actcggcgga
      841 tgcgccggcc gtcggcgtcg acgcactcga gccgccagcc cgacgggtgg acgaccgatt
      901 cgccgggctt gaggatccgc cccgcgatca ggaacaccag gcccccgagc gtgtcgatct
      961 cgtcttcctc cgcggtcagg cgggcgtcga ccgtcgccgt cagttcctcg agctcgagcc
     1021 gggcgtccgc ctcccacagg ccctcctcga gcaggatcag catccggccg gtttcctcgt
     1081 cgtgctcgtc ctcgatctcg ccgacgatct cctcgacgac gtcctcgatg gtcaccagcc
     1141 cttccgtgcc gccgaactcg tcgacgacga tggcgaggtg gatgtggtcg gcgcgcatcc
     1201 gcgccagaag gtcgatcacc cccatcgatt cggggacgaa caagggcgtg cgcatcagcg
     1261 agcccggcga gcggtcgcgg gtctcgtcga cgtcggcgat gaagacgtcc ttgatgtggt
     1321 acatgccgac gacctcgtcg aggctgtcgc cgtagaccgg gagccggctg tggccggcgt
     1381 cggcgaaggc gcggatcaaa tcctcgaagc cggtcgtcgc cggcaccgcg atgatgtcgc
     1441 cgcgggtcac gcagatgtcg ccggcggtgc ggtcgcccat ctgcagcagg ttgcggagca
     1501 tctgccgctc gtgcgggctg aggtcgccgg tgaccgggcg ggcggtctcg gcctcgtcga
     1561 tcgcttcctc gatctggtcg cgaaggctgg tctcgccgtc gtcgccgaaa atcagcgcgc
     1621 gcatcccgcg ccacagccgc gagccgacct cgtcctgctg ggtcgccatc agcgcgtccc
     1681 ctcatagggg tcggcgatgc cgagccgggc aagcgcccgg acctcgcgct gctccatcgc
     1741 cgcggccgac cgatcgtcct ggtggtcgca gccgagcagg tgcagcgtcc cgtggaccag
     1801 caggtgcgcc gcatggtcgg cgatcgggac cgccttggcc tcggcctcgg cggcgcacac
     1861 gccgtgcgcg aggacgacgt cgccgagcat cagctccggc ccgtcgcccg aagcggcctc
     1921 gagctcgccc ggctcggcca tcgtgaacga cagcacgttg gtcggccggt ccttgccgcg
     1981 ccactgctcg ttgagcgcct ggacttgccc gtcgccggtc agccggaccg aaagctcgac
     2041 cgggcgcggg ccggtcgcga ggtgcgggaa ggcgctttcg gcgatcgccg attccgcggc
     2101 ggcccgggcc agcgcctgcc agtcggtgct actgtcccat tccgcgtcgg tctcgatggc
     2161 gacctcgagc atcatgcgcg cctcacgctc cgggcccctc ataggcctcg acgatccggc
     2221 cgacgagcgg atggcggacg acgtcggcgg cggtgaagcg gatgacggcg aggctcttga
     2281 tgcccgacag gcgcgacacc gcgtcggcga gccccgactt gccggggtcg ggcaggtcga
     2341 cctgggttgg gtcgccgcag atcaccatcc ggctgcgcat cccgaagcgg gtcaggaaca
     2401 tcttcatctg cgccggcgtg gtgttttgcg cctcgtcgag gatgatgaag gcgtcgttca
     2461 aggtccgccc gcgcatgaag gcgagcggcg cgatctcgat ctcgcccgag gcgatccgcc
     2521 gctcgacctg ctcggtcggg agcatgtcgt agagcgcgtc gtagagcggc ctcagataag
     2581 ggtcgacctt gtccttcatg tcgccgggac ggaaaccgag gcgctctccg gcctcgaccg
     2641 cgggccgcga caggatcagc cggtcgacgc tgccgctgat cagctgcgac accgcctgtg
     2701 cgaccgcgag ataggtcttg ccggtgccgg ccgggccgag cgcgaagatc atgtcgtcgc
     2761 gggccagcgc ctccatgtag gcggtctgca cggtcgagcg cgggacgacg gtcttcttgc
     2821 gggtgcggat catcacccgc ggcggcgagg tgacctcgtc ctcgatgatc ccgtcgaggc
     2881 tcggctgggc ggccatggcg atcaccgatt cgaccgccgc ggcgtcgacg tcgtggccct
     2941 ggtcgagccg gttgtagagc ccgaccagca cgtcgcgcgc ccgcgccgcg gcgtcggcct
     3001 cgccctcgat ctggacccga ttgccgcgcg ccgcgatgtg caccccgagg cgctgctcga
     3061 tggcgatcag gtggcggtca tagtcgccga acagcggccc gagcaggtac ggctggtcga
     3121 actcgagctc gaggcgggcg cggtccggga ccgctgcaag gtcgcggcgg gccatcaggc
     3181 ggcagcccgg gcgttttggg cggcgggggc cgctccggcc atgctgttgg gaccagcggc
     3241 gaccagggtg acgtcgacca tgtcgccggg ctccgcggcg ctctcgaaat gcaccgactg
     3301 cagccatggg ctgcggccga tcatctggcc gcccttgcgg cccctgcgct cgaccagcac
     3361 cgacgtgcgc cggccgaccg aggctcggtt gaaggcgagt tggtgctcgg cgatccgcgc
     3421 ctgcaggcgc tgcagccgct cgtccatcac ttgcagagcg acctggccgt ccatcgccgc
     3481 cgccggggtg cccggccggg cgctgtactt gaacgaatag gccgccgcgt agccgaccgc
     3541 gtcgacgatg ctcagggtcg cctcgaagtc cgcatcggtc tcgcccggga agccgacgat
     3601 gaaatcgccc gaaaaggcga tgtcgggccg cgcggcacgg accttgtcga tcgtccgcaa
     3661 atagctgtcg gtcgagtggc ttcggttcat cgccttgagg atcctgtcgc tgcccgactg
     3721 caccggcagg tgcaggtacg gcatcagctt gccgacctcg ccgtgcgcgg cgatcaatcc
     3781 gtcgctcatg tcggccgggt ggctggtggt gtagcggatg cgctcgagcc catcgatccc
     3841 cgacaacgcg cggatcagcg catcgagccc gcggccgtca tcgttccagg cattcacgtt
     3901 ctggccgagc agcacgatct cgcgcgcgcc gccctcgacc agcgcccgcg cctcggcgac
     3961 gatatcgccc cacggccgcg agatctcggc gccgcgcgtg tagggcacga cacaataggt
     4021 gcagaacttg tcgcagcctt cctggacggt gaggaaggcg ctcggcgccg cccgccgccg
     4081 ccgcggcagc gcatcgaact tggacagggc cggcatggcg gtgtcgaccg gccggtcgcc
     4141 gcgggcagcc tgcgcgacca tctccggaag ccggtggtag gcctgcggcc cgacgactat
     4201 gtcgatcatc ttcgaccgcg ccgcggcctc cgccccctcg gcctgggcga cgcagccggc
     4261 gagcgcgatc agcggccgcg acccgtcctc gcgccgcagc cggccgatgt cgctataggc
     4321 cttttccgcc gctttctcgc ggatgtggca ggtgttgagg acgaccaggt cgggctcctc
     4381 gccgtcggcg gccggagcga ggccggtcgt tcccagcagc tcggccatgc gctcgccgtc
     4441 gtagacgttc atctggcagc cgaacgactt gatgcggaag cttttcaggg gcgaggtcat
     4501 gggtcggacg ggctataagc cagccggcgc gataccggaa ggcgcaagcg ccgcggcgat
     4561 cgcatcgtgc gcggccttgg ccatcgcctt gcggtcgagc gacgggtcga gcggctcgag
     4621 cagccgcacg gtcgcggtca tccgccccct tcgcccgagg atccggaccg cgttggcaag
     4681 cccgtgctcg cccctcgccc agcccagcgc cggcgtggcc gggccatagt cgatcgccgc
     4741 cggctggatc gcgcagcccg gcggcggcgg ggcgaccgcg gtcagcagtg cggcgcggaa
     4801 cgggagcagc cggccgccgt tcgcgaccgt tgcttcgggg aacagagcca gcggctggtc
     4861 gccggtcagc gccctgcgca ccgccctggt ctggtcgtgc agcccgcgcc ggtccgagcg
     4921 gtcgatgtag agcgtgcggt tctggtcggc gagccacttc atcaccgggt gccggctgag
     4981 ctcggccttg gacacgaacg cgcagccggt cgccgccgcg agcaccggca cgtcgagcca
     5041 gctggtgtgg ttggcgagca gcatcgactg ggaattggcg agcttgccct cgacctggac
     5101 atcgactccg cagatcgcgg ccgagccgga cagaaagcgg cgcggccagc gcgagcggcc
     5161 gaacagcttc gagaccagat gcgggacgac gcacagcagg aaccagccgc cgagcgcggc
     5221 gattcgcaat ccggcccgcc acttggccga gggcggctca gcggccgcgg tcgagggcga
     5281 cgccgtacag ctccatgcga tggtcgacga ggcggaagcc gagcttctcg gcgatccgct
     5341 tctgaagctc ctcgaggtct tcgtcgacga actcgatgac gttgccggtt tcgacgtcga
     5401 tcagatggtc gtggtgggcg tccgacgcgg cttcgtagcg cgagcggccg tcgccgaact
     5461 cgtgccgctc gaggattccc gcctcctcga acagccgcac cgtccggtag acggtggcga
     5521 tcgagatctt cgggtcgatc gcggcggcgc gctcgtggag cgtttcgacg tcggggtggt
     5581 cctccgcctc ggacaggatg cgcgcgatgg tccggcgctg ctcggtgatc cgcagcccct
     5641 tgtcggcgca cagggcctcg atgtcgatgt cgcggtgcat ggcccttagt taggcagtga
     5701 gcgcggcccg gtcaaaggta tcctccccgg cccggggagg aaccttctac ttggcgccgc
     5761 gcgtcttgcg ccgcttggtg cccagcccga tcgacttggc gagcgcccgg cgctgctcgg
     5821 cataattggg agcgaccatc ggatagtcgg cgttgaggcc ccacttctgc cggtactggt
     5881 cgggggtcat ctgataatgg gtcatcagat gccgcttgag catcttcagt cgttttccgt
     5941 cctcgaggca gacgatatag tcgggcttga ccgaagagcg gatcgggacc ttgggctccg
     6001 gcctcgcttc gggcgccgag cgcgtatcgg agatggtgct cagggcggcg tgaacgttct
     6061 ggataaggtt cggcaaatca ttgactgcaa cgctattgtt gctgacgtgc gccgcgacga
     6121 tgtctgcggt cagcgcgagt aaagtgtctt cggcaccttc attgtcgacc atcggatatt
     6181 tcccaagttg agcagagttc tgagcggctt cgccacctca gcgacaacag caataagagg
     6241 ctgctgttct tgacgcaata gatgcggaca attgacggcc gttagaatta tatcgaaaat
     6301 gcgagtgtaa tcgcgtcgta ttggcgccca tcgctgccgc gataatagtt gggccgccgg
     6361 ccggcgggct tgaatccggc caggcgatac atccggaccg ccggattgcc gtcgcggac
//
LOCUS       DASTZO010000214         8136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_850237,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000214 DASTZO010000000
VERSION     DASTZO010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 8136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8136
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850237"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1485
                     /locus_tag="VFU20_06020"
     CDS             166..1485
                     /locus_tag="VFU20_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008599558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5286052.1"
                     /translation="MNLRPFAAAAAAFALSFAAPAPAQLSRPEQAMIRTVDAEQERTV
                     AMLGRWVDQNSGTMNFEGVKAVGDMLRAELEPLGFKVEWIDMKAANRAGHLVARHKGN
                     GRGKRLLLIGHLDTVFEKDSPFQRWVRRGDVGEGPGAEDDKGGMAVIVAALRAMKAAG
                     TLKNADITVFLTGDEEDSGPVEISRRDLVAAGKWADVALDFEGLVQENGRDMGSTARR
                     SSDEWTVTVTARSGHSSGIFTPSAGSGAVYELARIIEAFRRELPEDKLTYNVGLVGGG
                     ATAQLDPDRIRISATGKTNIIAGTAIARGDLRAIDPGQIARTQAKMRAIVARPLPGAR
                     SEIDFPPDGYPPMAPTEGNRALLARLNAVNRDMALPDMGELDPVKRGAADISFVAKDV
                     DGLAGLGAAGGGAHAPGETVDIPSIFKQAKRAAILMSRLARERRGRH"
     gene            complement(1498..1833)
                     /locus_tag="VFU20_06025"
     CDS             complement(1498..1833)
                     /locus_tag="VFU20_06025"
                     /inference="COORDINATES: protein motif:HMM:NF016149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance CopC family protein"
                     /protein_id="HEU5286053.1"
                     /translation="MALLGSCAAAPPAEPAATAQSILQSSTPADGSTVQGPVDSLELR
                     FSPPARLHELTVTGSDGLTMPTMVTTVGEVPHYSIPLPGLGAGAYTVNWRATAAGASH
                     RGSFRFTVR"
     gene            complement(1923..2423)
                     /locus_tag="VFU20_06030"
     CDS             complement(1923..2423)
                     /locus_tag="VFU20_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5286054.1"
                     /translation="MELKFRVAARIARPVHEVFEAVADPNQLSQYFTTGGAKGRLVAG
                     ATVTWDFHDFPGEFPVEVIEVVPDEKIVLRWDAAEGEQAVADFDDGKQPRITTADYKT
                     TVTMSFTPIDGGSRTLVEIREVGWRETQGGLDASYGNCMGWSQMLCALKAWLEHGLNL
                     REGMYQ"
     gene            2480..2806
                     /locus_tag="VFU20_06035"
     CDS             2480..2806
                     /locus_tag="VFU20_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5286055.1"
                     /translation="MSISDRFDRTFKALAAPIRRQILDQLRDQPLTTGMLCALFPELD
                     RCTVMQHLKVLEESELVIAVRKGRERWNHLNPLPIHEIHERWIGPHAARATVRLAQLK
                     RELERR"
     gene            2934..3419
                     /locus_tag="VFU20_06040"
     CDS             2934..3419
                     /locus_tag="VFU20_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286056.1"
                     /translation="MDDDVQQPTVMEDSAPARPPMHLWIVGALALLWNGFGCYDYLMT
                     RMRNTDYLAEMMPTVDPEAALAWVDAFPVWAQFGWGLGVWGGLAGAVLLLLRHRWALW
                     AFGLSFVGAILGLGYQIVAAPPLPGADDPLFKVMPYVIILVAAALALYASAMRKKGVL
                     R"
     gene            3612..4052
                     /locus_tag="VFU20_06045"
     CDS             3612..4052
                     /locus_tag="VFU20_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HEU5286057.1"
                     /translation="MASIWPAADPALVEPPVFLASEPEKVDQHFSRCGPGRGHACVID
                     GDTFKLGKRKVRIIGIDAPEVKARCPEEAAKAEASTAELQRLLNQGPFEMVGRIDDRQ
                     DRYGRDLRAIRRIRPNGTKQSIAEDMRASGLARRYWGGLKSSWC"
     gene            4114..4431
                     /locus_tag="VFU20_06050"
     CDS             4114..4431
                     /locus_tag="VFU20_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286058.1"
                     /translation="MTRPAEAKPEPARPERDWTRVQDYLDLARLWGRAGERHRRRMRP
                     RTEASAPRLLSLGMLPFVLLFAAMAVLAFLIILAAVPGKRYPERPPPPPEAGTAPPGW
                     IDG"
     gene            complement(4566..4949)
                     /locus_tag="VFU20_06055"
     CDS             complement(4566..4949)
                     /locus_tag="VFU20_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286059.1"
                     /translation="MRIMVLGFAAPLAAVALPAAPAVALDPASPQFTVGAAAGVTVHR
                     GQPGRFDRGFRRDGDRDDRRRHRGGDDFVFIYDRDWQGDTAWRANSYNDWWHERPWRA
                     YPRWVRSNQDCERVWQGGGTWRCEW"
     gene            5088..5609
                     /locus_tag="VFU20_06060"
     CDS             5088..5609
                     /locus_tag="VFU20_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286060.1"
                     /translation="MPAGLSLAIALLAAAAPPASGQPAPPSPPESAPGKGANGCSPAP
                     EAIEPGEIFVCAPRPQGYRIDPDLLEAQRAKRSGRPKRPERMADTSCASVGPFGCTYG
                     AGIDLLGAAMVLGTMATKAVRGENVGEMFVTDPQRSEYDHYLEAKRLREAKEAEAAAA
                     AAKAKDPKPSGSK"
     gene            5705..6529
                     /locus_tag="VFU20_06065"
     CDS             5705..6529
                     /locus_tag="VFU20_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286061.1"
                     /translation="MPYRHAPYYVLFVIAVIALGFWPSYFAKWGSVPWQFHAHGVAAS
                     LWVGMVLAQSIAVHKAQLPLHRAVGKSSLLLFPFLIGGLAAIIDLTGKTVVAGPDPVR
                     TMFGSSFVVGLALAIAAYVLLYYRALKYRRKVWLHSGYMLATPLILFESPFGRILNGY
                     LPGLAIRGPDDLHLIMPAILSSMALELAVIAAIWLKYRAQASPFLVAGGFIVAQMLTM
                     GLLANSTLLQPVLVAIGQTPSAAVVLAGFAIGAATSWAGWQAGKRPAVPVAAAAAA"
     gene            6723..6980
                     /locus_tag="VFU20_06070"
     CDS             6723..6980
                     /locus_tag="VFU20_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286062.1"
                     /translation="MASPLQTAKQPVDLAAGGARPSRIRRDPPARTDKKTVVPDRDER
                     DQWTVTLGVLAFTLAIVAIIAGFSAVTGWSPSDRVVHIRLE"
     gene            complement(7192..7950)
                     /locus_tag="VFU20_06075"
     CDS             complement(7192..7950)
                     /locus_tag="VFU20_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286063.1"
                     /translation="MGKPSKQQIADAIIHSAKAGESMADFDARIAGLSRISDEELSAI
                     GVAPPTCPEPADGPANIVPAPIAPVDPDPPAFAPAPLRPRRDGWTAERQRAFIAALAE
                     TGCVSEACAEVGITPRSAYRLREHAAAAGFRVAWEHAESLATARLTALAFERAVHGSV
                     ERIYRDGVLVEERRKPSDKLLMWLLSHRDPVGYGWLTRPPRETPHSSFYPLQSARSEL
                     PHLLGGLADIGPEACAAEPIPGEALDYDAEPPRA"
BASE COUNT         1223 a         2805 c         2863 g         1245 t
ORIGIN      
        1 gattcgtcca ctggtcattc tccaccccgg aattcttccc agtcgcaggt aagtaaccag
       61 ataggttggc tgtaggacag caaattcgcc aaaaacattg gttgcgcaga acgcccttac
      121 ccaaaacggc cttgcctcag ctcggcggca ggctaggctg cgcccatgaa cctccgccca
      181 ttcgcggccg ccgccgccgc tttcgccttg tcgttcgccg cccccgcccc ggcccagctg
      241 agccggccgg agcaggcaat gatccggacc gtcgacgccg agcaggagcg gaccgtcgcg
      301 atgctcgggc ggtgggtcga ccagaacagc ggcacgatga acttcgaagg ggtgaaggcg
      361 gtcggcgaca tgctccgggc cgagctcgag ccgctcggct tcaaggtcga gtggatcgac
      421 atgaaggcgg ccaaccgcgc cggccatctc gtcgcccgcc acaaggggaa cgggcgcggc
      481 aagcggctgc tcctgatcgg ccatctcgac acggtgttcg agaaggattc gccgttccag
      541 cgctgggtcc gccgaggcga cgtcggcgaa ggcccgggag ccgaggacga caagggcggg
      601 atggcggtga tcgtcgccgc gttgcgggcg atgaaggcgg cggggactct caaaaatgcc
      661 gacatcaccg tgttcctgac cggcgacgag gaggattcgg gcccggtcga aatcagccgg
      721 cgcgacctgg tcgcggccgg caaatgggcc gacgtggcgc tcgatttcga agggctggtg
      781 caggagaacg ggcgcgacat gggctcgacc gcgcggcgca gctccgacga gtggacggtg
      841 acggtcaccg cgcgcagcgg ccattcgagc ggcatcttca ccccgagcgc gggctcggga
      901 gcggtctacg agctggcgcg gatcatcgaa gcgttccggc gcgagcttcc cgaggacaaa
      961 ttgacctaca acgtcggcct ggtcggcggc ggagcgaccg cccagctcga cccggaccgg
     1021 atccggatct cggcgaccgg caagaccaac atcatcgccg ggaccgcgat cgcccgcggc
     1081 gacctgcgcg ccatcgaccc cgggcagatc gcccgcaccc aggcgaagat gcgcgcgatc
     1141 gtcgcccggc cgctgcccgg cgcccgctcg gagatcgact tcccgcccga cggctatccg
     1201 ccgatggcgc cgaccgaggg caaccgcgcg ctgctggccc ggctcaacgc ggtcaaccgc
     1261 gacatggcgc tgcccgacat gggcgagctc gacccggtca agcgcggcgc cgccgacatc
     1321 agcttcgtcg ccaaggacgt cgacgggctc gccggcctcg gagcggccgg cggcggcgcc
     1381 cacgctccgg gcgagacggt cgacatcccg tcgatcttca agcaggccaa gcgcgcggcg
     1441 atcctgatgt cgcggctggc gagggagcgg cgcgggcgcc actgatttcg gccgcgctca
     1501 gcggacggtg aagcggaagc tgccccggtg tgacgcgccg gcggcggtcg cccgccaatt
     1561 gacggtgtag gcgcccgcgc caaggccggg cagcgggatc gaataatgcg gcacttcgcc
     1621 gacggtcgtg accatcgtcg gcatggtgag gccatcgctg ccggtgacgg tcagctcatg
     1681 gagccgcgcc ggcggcgaga agcgcagctc cagtgaatcg accgggccct ggaccgtcga
     1741 accatcggcc ggcgtcgagc tttgcaggat cgattgggcg gtggcagcgg gctcggcggg
     1801 gggcgcggcc gcacaggaac cgagcaaggc catcgccgca aacgccccca gggctttcga
     1861 gcttgcatgc gccatcggtc agtgaaccgg ctcgacacac acaggttccg tcgacccggc
     1921 ggctattggt acatgccctc gcgcaggttg aggccgtgct cgagccacgc cttcaatgcg
     1981 cacagcatct gcgaccagcc catgcaattg ccgtagctcg cgtcgaggcc tccctgcgtt
     2041 tcgcgccagc ccacctcgcg gatctcgacg agggtgcggc tgccgccgtc gatcggcgtg
     2101 aaactcatcg tcaccgtcgt cttgtagtcg gcggtcgtga tccgcggctg cttgccgtcg
     2161 tcgaagtcgg cgaccgcctg ctcgccctcg gccgcgtccc agcgcagcac gatcttctcg
     2221 tcgggcacga cctcgatcac ctcgaccggg aactcgccgg ggaaatcgtg gaagtcccag
     2281 gtcacggtcg cgcccgccac cagccggccc ttggcgccgc cggtggtgaa atattgcgac
     2341 agctgattgg ggtcggcgac cgcctcgaac acctcgtgga cgggcctcgc gatccgagcc
     2401 gcgaccctga acttgagttc catgacaagc ctccgctcgc gtgcttgagt cgtgcgcaaa
     2461 tacgttatag gattataaca tgtcaatcag cgaccgcttc gaccgcacct tcaaggcgct
     2521 cgcggcgccg atccggcgcc agatcctcga ccagctgcgc gaccagccgc tgaccacggg
     2581 catgctgtgc gcattgttcc ccgagctcga ccggtgcacg gtgatgcagc atttgaaggt
     2641 gctcgaggag tctgagctgg tgatcgcggt gcgcaagggg cgcgaacgct ggaaccacct
     2701 caacccactg ccaatccacg agatccacga gcggtggatc ggcccgcacg cggcgcgcgc
     2761 gacggtccgt ctggcgcagc tgaagcgcga gctcgagcgg cgatagacgc cgcgcttgac
     2821 ttcgattccg cgatggagcc tgatccgccg cgccggggcc ttcgccgctt ccggccaggt
     2881 gccgcgtcgg ccggcagcgc cgcccgctgc ctcgagcttg agggggacca gcgatggacg
     2941 acgacgtgca gcaaccgacc gtcatggaag attccgcgcc ggcgcgcccg ccgatgcatt
     3001 tgtggatcgt cggcgccctg gcgctgctgt ggaacggctt cggctgctac gactatctga
     3061 tgacccggat gcgcaacacg gattatctcg cggagatgat gccgaccgtc gatcccgagg
     3121 cggccctcgc ctgggtcgac gccttcccgg tctgggccca gttcggctgg ggcctagggg
     3181 tctggggagg cctcgccggg gcggttctgc tgctgcttcg ccaccgctgg gcgttgtggg
     3241 cgttcggtct gtcctttgtc ggggcgatcc tcgggctcgg ctaccagatc gtcgccgccc
     3301 cgccgctccc cggcgcggac gacccgctgt tcaaggtcat gccttacgtg atcatcctgg
     3361 tcgcggccgc gctggccctt tacgcgagcg cgatgcgcaa gaagggcgtg cttcgctagg
     3421 cggccggctc gagtgagcgg tgcggcgcgc tcggtccgat gggcagaatc atccgcttcc
     3481 gtagccgcag atggacacgt tcgagcggtt acgggcatcg cgacctccgc cgcaaagtca
     3541 ctctgcgcgg cctcgcccgg gagaccgccg gctggcttgg caagcttcga ccgttcatcc
     3601 tccttggcat cgtggcttcc atctggcccg ccgcggaccc ggcgctggtc gagccaccgg
     3661 tgttcctggc gagcgagccc gagaaagtgg accagcactt cagccgctgc gggccggggc
     3721 gcggacatgc ctgcgtgatc gacggcgaca ccttcaagct tgggaagcgc aaggtgcgga
     3781 tcatcggcat cgacgcgccg gaggtgaagg cgcgctgccc ggaggaagcc gcgaaagccg
     3841 aagcgtcgac cgccgagctg cagcggctgc tcaaccaggg gccgttcgag atggtcggcc
     3901 ggatcgacga ccgacaagac cgctacggcc gcgacctgcg cgccatccgc cggattcggc
     3961 cgaatgggac caaacagtcg atcgccgagg acatgcgcgc cagcgggttg gcgcggcggt
     4021 attggggcgg gctcaagagc agttggtgct gacgggcggc tgtccggcac ccatccgttg
     4081 catcgcggcg cggcgctccc atcttgtcag gtgatgacca ggccggcgga agcgaagccc
     4141 gagcccgcgc gtccggagcg ggactggacg cgagtccagg attatctcga cctggcgcgg
     4201 ctgtggggcc gcgccggcga gcgccaccgg cgccggatga ggccgcgcac cgaagcctcg
     4261 gcgccgcgct tgctgtcgct cggaatgctg ccgttcgtgt tgctgttcgc ggcgatggcc
     4321 gtgctcgcct tcctgatcat cctcgccgcg gttcccggca agcgctaccc cgagcgcccg
     4381 cccccgccgc cggaagccgg caccgcaccg cccggctgga tcgacggctg acagcggggg
     4441 ccgtcacgaa ctgaattcgt gacgtcgatc gacacgcctg aagcggtcgt cggccggttc
     4501 tcgcagtctc ttcgcgccat tcgaggattg cgccgcaatc cgctcactgc gctcggcagc
     4561 tcgaactacc actcgcagcg ccaggtgccg ccgccctgcc agacgcgctc gcaatcctgg
     4621 ttgctgcgga cccagcgcgg gtaggcgcgc cacgggcgct cgtgccacca gtcgttgtag
     4681 ctgttggccc gccacgccgt gtcgccctgc cagtcgcgat cgtagatgaa gacgaaatcg
     4741 tcgccgccgc ggtgccggcg ccggtcgtcg cggtcgccgt cgcggcggaa gccacgatcg
     4801 aagcgccccg gctggccacg gtgaaccgtg accccggcgg cggcgccgac ggtgaattgc
     4861 gggctcgccg ggtcgagcgc gacggcgggg gcggcgggga gggcgacggc ggccagcggc
     4921 gccgcgaagc caagcaccat tatacgcatg ccgcttactc cttcgggttc ccctccggac
     4981 gggaagcaaa cgccgcagcg ggcgcggcgt tgcatggcaa gaagcccggc agcggcggcg
     5041 gcggacattc attgcgccgc aagctcgcag cgcacatgga tagcgccatg ccggccggcc
     5101 tatccctcgc gattgccttg ctcgccgcgg cagcgccgcc cgcttccggc cagccggcac
     5161 cgcccagccc gcccgaatcc gcgccgggca agggggccaa cggctgctcg cccgccccgg
     5221 aagccatcga gccgggcgag atcttcgtct gcgccccgcg gccgcagggc taccggatcg
     5281 accccgacct gctcgaggcg cagcgggcca agcgcagcgg ccggccgaag cggcccgagc
     5341 ggatggccga caccagctgc gcgagcgtcg ggccgttcgg ctgcacgtac ggcgccggga
     5401 tcgacctgct cggggcggcg atggtgctgg ggaccatggc caccaaggcg gtccgcggcg
     5461 agaatgtcgg cgagatgttc gtcaccgacc cgcagcggtc cgaatatgat cattacctcg
     5521 aggccaagcg gcttcgcgag gcgaaggagg ccgaagcggc cgcggcggcg gcgaaggcaa
     5581 aggatccaaa gccaagcggc tccaaataag cgcgattttc cggctttcgg cgggcgccgg
     5641 ggcggggtat tggcggtttc cgcggcccgc aaaagccgca gcggcacgga cacaaggggg
     5701 ctccatgcca taccgccacg cgccttatta cgtgctcttc gtcatcgccg tgatcgcgct
     5761 cggcttctgg ccgagctatt tcgccaaatg gggcagcgtc ccgtggcaat tccacgccca
     5821 cggggtcgcg gccagcctgt gggtggggat ggtgctcgcc cagagcatcg cggtgcacaa
     5881 ggcgcagctg ccgctgcacc gcgcggtcgg caagtcgagc ctgctgctgt tcccgttcct
     5941 gatcggcggg ctggcggcga tcatcgattt gaccggcaag acggtcgtcg cgggcccgga
     6001 cccggtgcgg acgatgttcg gaagctcctt cgtggtcggg ctggcgctgg cgatcgccgc
     6061 ttatgtcctg ctctactacc gggccctcaa ataccggcgg aaggtgtggc tgcactccgg
     6121 ctacatgctg gcgaccccgc tgatcctgtt cgaatcgccg ttcggccgga tcctcaacgg
     6181 gtacctgccc ggcctcgcga tccgcgggcc ggacgatctg cacctgatca tgccggcgat
     6241 cctctcatcg atggcgctcg agctcgcggt catcgccgcc atctggctca agtaccgggc
     6301 ccaggcgagc ccgttcctgg tcgcgggcgg cttcatcgtc gcgcagatgc tcaccatggg
     6361 cctgctggcg aactcgaccc tgctgcagcc ggtcctggtg gcgatcggcc agacgccgag
     6421 cgccgcggtg gtgctggccg gcttcgcgat cggcgcggcg accagctggg ccggctggca
     6481 ggcgggcaag cggcccgcgg tgccggtcgc ggcggcggcg gcggcgtagg ggcgagcggg
     6541 cggcgacgcg atgggccgtc acggagtgaa tccgtgacgt cgatcgacac ggcgtcgccg
     6601 gtcgtcggcc ggatctcgcc gtctgtgcgc gctgcctcgt gatcgcttcg caatctctcc
     6661 gctgcgcgcg gcggctcgat cactaacgca atcataaccc attgcggctt agcaaggggg
     6721 ccatggccag cccgctccag accgcaaagc agcccgtcga cctggcggcg ggcggcgcgc
     6781 gcccctcgcg catccgccgc gacccgccgg cgcggaccga caagaagacc gtggtccccg
     6841 accgcgacga gcgcgaccag tggaccgtga cgctcggagt cctggccttc acgctggcga
     6901 tcgtcgcgat catcgccggg ttcagcgccg tcaccggctg gtcgccgagc gatcgcgtcg
     6961 tccatatccg gctggagtag gggaccgaag caaagcgtca cgggcggccg tgaggtgacc
     7021 ttcggtcagc ttcgcttccg atcgggttcg gcgttgccga cccgtcggcc ggctttcgcg
     7081 cactcttcga cgccgctcca cgattgctgc gcaatcctgt cgctgcgctc gggcgctcga
     7141 gctggaaagt cgcaaaagtc gcactgcaac gtgcgcgcgg gcgcgggcgc gtcacgcgcg
     7201 cgggggctcg gcatcgtaat ccagcgcctc gccggggatc ggctcggccg cgcaggcttc
     7261 gggaccgatg tccgccagcc cgccgagcaa gtgcggcagc tcgctgcgcg ccgactgcaa
     7321 cgggtagaag gacgaatgcg gcgtttcccg gggcgggcgg gtcagccagc cgtagccgac
     7381 cgggtcgcgg tgcgagagca gccacatcag caatttgtcg ctgggcttgc gccgctcctc
     7441 gaccagcacc ccgtcgcggt agatgcgctc gacgctgccg tggaccgcgc gctcgaacgc
     7501 cagagcggtg agccgcgcgg tagccaggga ttcggcgtgc tcccaggcga ctcggaagcc
     7561 ggccgcggcg gcatgctcgc gcaggcgata ggcgctgcgc ggggtgatcc cgacctcggc
     7621 gcaggcctcg ctgacgcagc cggtttcggc cagagcggcg atgaacgcgc gctgccgctc
     7681 ggcggtccag ccgtcgcggc gcgggcgaag gggcgcgggc gcgaacgccg gcgggtccgg
     7741 gtcgaccggc gctatcggcg cggggacgat gttggccggc ccgtcggcgg gctcaggaca
     7801 ggtaggcggg gcgacgccga tcgcgctgag ctcttcgtcg gatatccggc tcagcccggc
     7861 gattcgcgcg tcgaagtcgg ccatgctttc gcccgctttg gccgaatgga tgatcgcgtc
     7921 ggcgatctgc tgctttgatg gcttgcccaa attcctgatc cttctggttc ctctaatacg
     7981 cgtaccatga accaaatagg atgctgtagg aaagtttctt ttgttgtgcc gacgtttgcc
     8041 accaagtgaa ggccgcccgg agagcactgt tgatgtggca gtcgccgagt agttatggcg
     8101 acacgttacc gactctcagt tttggagttc ctgcaa
//
LOCUS       DASTZO010000215         2113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1115477,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000215 DASTZO010000000
VERSION     DASTZO010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1115477"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..238)
                     /locus_tag="VFU20_06080"
     tRNA            complement(152..238)
                     /locus_tag="VFU20_06080"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(202..204),aa:Leu,seq:cag)
     gene            330..1286
                     /locus_tag="VFU20_06085"
     CDS             330..1286
                     /locus_tag="VFU20_06085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HEU5286064.1"
                     /translation="MIERGDELVTVFGGGGFIGRYVCELLLKSGVRVRVASRRPSRAH
                     FIQPLGQVGQFGFVRADVTDRDSVARALDGASAAINLCGILKGRFRAVHVTGARNVAE
                     AARDAGAGALVHISAIGSDPGSEADYGKTKGEGETAVRAAFPGATIIRPSLVFGPEDQ
                     LTNRLAGLARLPFLPVIAARRRFQPVYVRDLALAIARAALDPEQHGGKTYEIGGPQVL
                     SMRELHSAILESTGQKPELVDMPNFMASLLAWFGWLPGAPLTRDQWLMLQRDNVAAKG
                     APGLEAFGIKPTPLGAVAHEWLGRFHKGGRFAGRRINLTATS"
     gene            1309..2109
                     /locus_tag="VFU20_06090"
     CDS             1309..2109
                     /locus_tag="VFU20_06090"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU5286065.1"
                     /translation="MPVLILVILLGIVEGVTEYLPVSSTGHLILATELLGFDADAWAV
                     FNIAIQPGAILAVVVLYWRTFRDVVVGLFQRSPSAFAFVRNILLAFLPAAVLGVVLGN
                     YIEMLLGNAVVVGWALIVGGIAILVVERFARPQDCGGVAAVSMRQSAMIGLVQCLAMI
                     PGVSRSGATILGAMSFGVDRKTAAEFSFFLAVPTLTGATALQLFKHWDDIPTGEFAWI
                     GLGSLVSFVVAVIVVKAFMAIVTRYGFAPFAWYRIVAGSAALIWLGLR"
BASE COUNT          301 a          727 c          719 g          366 t
ORIGIN      
        1 ttccgcgccg aaaaggactt cggcgaaggc ctggccgagc ttgccgaatg ggtggcccgg
       61 cagaccgccc acgaccgcat ccaccaggcg cgcgccgagc tggaagcgaa aggcctggtc
      121 gcctgaggcg acccgatcaa ctttaagaga atggtgccca ggagaggact cgaacctcca
      181 cgcccttgcg agcgccagca cctgaagctg gtgcgtctac caattccgcc acctgggcac
      241 gatgtcatgg atgtagtcgg taggcgcggg cgcttaaagg cgcggctccc gccttgtcaa
      301 tggcgaggcc gagccgctag tgcggcgcga tgatcgagcg cggcgacgag cttgtaacgg
      361 tgttcggagg cggcggattc atcggccgct acgtctgcga gctgctgctg aaaagcggcg
      421 tgagggtgcg ggtcgcgagc cgccggccga gccgcgccca cttcatccag ccgttgggcc
      481 aggtcggcca gttcggcttc gtccgggccg acgtcaccga ccgcgacagc gtcgctagag
      541 cgctcgacgg cgcctcggcg gccatcaacc tgtgcggaat cctcaaaggc cgtttccggg
      601 cggtgcacgt gaccggggcg aggaatgtcg ccgaagcggc ccgcgacgcc ggcgccgggg
      661 cgctggtcca catctcggcg atcggctccg atcccggttc cgaagccgac tatggcaaga
      721 ccaaaggcga gggcgagacc gcggtccgag ccgccttccc gggcgcgacg atcatccgcc
      781 cgtcgctggt gttcgggccc gaggaccagc tcaccaaccg cctcgccgga ctcgcccggc
      841 tgccgttcct gccggtgatc gccgcacggc gccgcttcca gccggtctat gtccgcgacc
      901 ttgctttggc gatcgccagg gcggccctcg acccggagca gcatggcggc aagacctatg
      961 agatcggcgg gccgcaagtg ctgagcatgc gcgagcttca ctcggcgatc ttggaatcga
     1021 ccggccagaa gcccgagctg gtcgacatgc ccaatttcat ggcttcgctg ctggcctggt
     1081 tcggctggct cccgggagcg ccgctgactc gcgaccagtg gctgatgctg cagcgcgaca
     1141 atgtggcggc caagggcgct ccgggcctcg aggcgttcgg gatcaagccg actccgctgg
     1201 gagcggtcgc ccacgagtgg ctcggccgat tccacaaggg cgggcgcttc gcgggccgcc
     1261 gaatcaacct gaccgccacg tcctaggacc ggcagcgagc ctcgcccgat gccggtcctg
     1321 atcctcgtca tcctgctcgg catcgtcgag ggcgtgaccg aatatctccc ggtctcgtcg
     1381 accggccact tgatcctcgc gaccgagctg ttgggcttcg acgccgacgc ctgggcggtc
     1441 ttcaacatcg ccatccagcc gggcgcgatc ctcgcggtgg tcgtgctcta ctggcgcacg
     1501 ttccgcgacg tggtcgtcgg cctgttccag cgctcgccgt cggcattcgc cttcgtccgc
     1561 aacatcctcc tcgccttcct tcccgcagcg gtgctcgggg tcgtgctcgg caattacatc
     1621 gagatgctgc tcggcaatgc ggtcgtggtc ggctgggcgc tgatcgtcgg cgggatcgcg
     1681 atcctggtcg tcgagcgctt cgcgcggccg caggactgcg gcggagtcgc cgcggtctcg
     1741 atgcggcaat cggcgatgat cggcctcgtc cagtgcctgg cgatgattcc cggggtcagc
     1801 cgctcgggcg ctaccatcct cggggcgatg agcttcgggg tcgaccgcaa gaccgccgcc
     1861 gagttcagct tcttcctcgc cgtcccgacc ctgaccggag ccaccgcgct gcagctgttc
     1921 aagcattggg acgacatccc gaccggcgag ttcgcgtgga tcgggctcgg cagcctggtc
     1981 tcgttcgtcg tcgcggtgat cgtcgtcaag gcgttcatgg caatcgtcac ccgctacggc
     2041 ttcgcgccgt tcgcctggta ccggatcgtc gccggctcgg cggcgctgat ctggcttggc
     2101 ctgcggtgat tta
//
LOCUS       DASTZO010000216         3685 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_697097,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000216 DASTZO010000000
VERSION     DASTZO010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3685)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3685)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3685
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_697097"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            586..1620
                     /gene="recA"
                     /locus_tag="VFU20_06095"
     CDS             586..1620
                     /gene="recA"
                     /locus_tag="VFU20_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU5286066.1"
                     /translation="MDRQKALEAALAQIDRAFGKGSAMKLGSREKIEIDTVSTGSLGL
                     DIALGVGGFPRGRVVEIYGPESSGKTTLALHAIAEAQKVGGSAAFVDAEHALDPAYAK
                     KLGVDIDELIVSQPDTGEQALEIVDTLVRSNAIDVLVIDSVAALVPRAEIEGEMGDSH
                     VGLQARLMSQALRKLTGSISRSRCTVIFINQVRMKIGVMYGNPETTTGGNALKFYASV
                     RLDIRRIGQIKDRDEIVGNATRVKVVKNKVAPPFKQVEFDIMYGEGVSKVGEILDLGV
                     KAGLIEKSGAWFSYDSVRIGQGRENAKLYLKENPDVAQRIENAIRGKTDEVAEALMIG
                     ADAIEDDVEA"
     gene            complement(1716..3017)
                     /locus_tag="VFU20_06100"
     CDS             complement(1716..3017)
                     /locus_tag="VFU20_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HEU5286067.1"
                     /translation="MNRPRASTSGRSTRAAAVQRLPGGREMTALLAGLMALNAFAIDA
                     MIPALPAIGESLSVEQENHRQLVVVAYMIGFGSTQLVWGPLADRFGRKPVLAAGVALY
                     AAFALLCGIAGSFTVLIAARALQGASAAVTRVLVVAMVRDLFRGEAMARMMSLVFMVF
                     MVVPVLAPSIGQAILLVAPWRWIFLVLAAYALIMLAWSWVRLPETLHPEYRRSLAWRD
                     MASAAWATIREPQSRGYTLALTITFGSLTAYIASVQQIVFDSFDAPNAIGLAFAAVAA
                     PMALASWTNSKVVGRYGLRRVGHGGAVAFAAVTLVHALIGAAGGETLAVFIVLQGLAM
                     ACFALTSSNMGTLAMEHMGPIAGTASSVQGVVGTIGGALIGLMIGQAFDGTAQPFLWG
                     TAACAMLSLIIVLLTEPKRLFAGVDRGAQAEAMPLTPPPEI"
     gene            3086..>3685
                     /locus_tag="VFU20_06105"
     CDS             3086..>3685
                     /locus_tag="VFU20_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008602473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HEU5286068.1"
                     /translation="MTHLGRRTFLTGSAAASAATLLGGASFHSARAAVADPAPIVLPP
                     PIGRAERLERLARARTLMKRHGIGAILIEPGASLDYFTGVQWWRSERLTAAVIPAEGE
                     PIVVTPFFEKPSVEESLAIPAEVRVWQEHEEPLKLVADFLRERGVARQPVGFEETNRF
                     FILDRLKEQLPRAQIVSANPIVRSLRMIKSPAELALMQAA"
BASE COUNT          627 a         1258 c         1267 g          533 t
ORIGIN      
        1 gaggccaagg ccttcgggat catcgacgag gtcttcgaca agaggcccga aagcggcgag
       61 ggcgacaaga ccggggcggc cgacatcact ccggtctgat cggcgaggcg agtcgccctt
      121 cgggagcctg gcccgtcgaa agttatccac agctttttgg cgccgctcgc cgctcggccc
      181 aaaaaaaggg cttgcgcgat ccggttcgtc ggctagggtg gcttcgtgct ttgggccctc
      241 gggtccgaag catgggaacc ccgccgggtc aaaccggtgg ggtttaccat ttctgggccc
      301 ccgcgaccgt tcgcgcccgc gcccagcacg ccagccacat cgccgatctc gtcccgccgg
      361 agccgccttc ggcgaccgag gcgctgcgcc ttttgcagcg gcttttcctt ggtcaatgtt
      421 cccctcttgt tctcgcggaa caaagcgtat acacagctct tctcgcgggg cggggctcga
      481 ccgccgcatg gaagaggcca gatcggcatg gcgccgattg acgaaaggac taaggtcatg
      541 tcagcgcagc ttaaagtggt cgcagcaggg ttggattcgg gcaatatgga caggcagaag
      601 gcgctcgagg cggcgctcgc gcagatcgac cgggcattcg gcaagggctc ggcgatgaag
      661 ctcggcagcc gcgagaagat cgagatcgac acggtctcga ccggcagcct cggcctcgac
      721 atcgccttgg gcgtcggcgg cttcccgcgc ggccgcgtgg tcgagatcta cgggcccgag
      781 agctcgggca agacgaccct tgcgctgcac gcgatcgccg aggcgcagaa ggtcggcggc
      841 agcgccgcct tcgtcgatgc cgagcatgcg ctcgacccgg cctacgccaa gaagctcggg
      901 gtcgacatcg acgagctgat cgtctcgcag cccgacaccg gcgagcaggc gctcgagatc
      961 gtcgacacgc tcgtccgctc gaacgcgatc gacgtgctgg tgatcgactc ggtcgccgcg
     1021 ctggtgccgc gggccgagat cgagggcgag atgggcgaca gccacgtcgg cctccaggcg
     1081 cggctgatga gccaggcgct gaggaagctg accggctcga tcagccgctc gcgctgcacg
     1141 gtcatcttca tcaaccaggt gcggatgaag atcggcgtca tgtacggcaa ccccgaaacg
     1201 acgaccggcg gcaacgcgct caaattctac gcgtcggtgc ggctcgacat ccgccgcatc
     1261 ggccagatca aggaccgcga cgagatcgtc ggcaatgcga cccgggtgaa ggtggtcaag
     1321 aacaaggtcg cgccgccgtt caagcaggtc gagttcgaca tcatgtacgg cgagggcgtg
     1381 tcgaaggtcg gcgagatcct cgacctcggc gtcaaggccg gcctgatcga gaagtcgggc
     1441 gcctggttca gctacgattc ggtgcgcatc ggccagggcc gcgagaacgc caagctgtac
     1501 ctcaaggaaa accccgacgt cgcgcagcgg atcgagaatg cgattcgcgg caagaccgac
     1561 gaggtcgccg aggcgctgat gatcggcgcc gacgcgatcg aggatgacgt cgaggcgtaa
     1621 ggctctcacc ctaacgtcgc tgcgctcctc ctccctctcc cgcgtgcggg gagggttggg
     1681 gtgagggtct ttgcccttgg attaggtcct ctgccctaaa tctcgggcgg cggagtcaac
     1741 ggcatcgctt cggcctgggc tccccggtcg acgccggcga acagccgctt cggctcggtc
     1801 aggagcacga tgatcagcga aagcatggcg cacgcggccg ttccccacag gaacggctgc
     1861 gcggtgccgt cgaacgcctg gccgatcatc agcccgatca gcgctccgcc gatggttccg
     1921 acgactccct ggaccgagga ggcggtgccg gcgatcggcc ccatatgctc catcgccagc
     1981 gttcccatgt tcgacgaggt cagggcgaag caggccatcg ccagcccctg caggacgatg
     2041 aacaccgcca gggtctcgcc gcccgccgcg ccgatcaggg cgtggaccag ggtcacggcg
     2101 gcgaacgcga ccgcgccgcc gtggccgacc cgccgcagcc cgtagcggcc gaccactttc
     2161 gaattggtcc aggacgcgag cgccatcggc gccgcgaccg ccgcgaacgc gaggccgatc
     2221 gcattgggcg catcgaacga gtcgaacacg atctgctgga ccgaggcgat gtaggcggtg
     2281 aggctgccaa aggtgatggt cagcgccagc gtgtagccgc gcgactgcgg ctcccggatc
     2341 gtcgcccagg cggcgcttgc catgtcgcgc caggcaagcg agcggcggta ctcgggatgc
     2401 agcgtttcgg gcagccgcac ccacgaccag gcgagcatga tcagcgcgta ggccgccagc
     2461 accaggaaga tccaccgcca cggcgccacc aaaaggatcg cctggccgat gctcggcgcc
     2521 agcaccggca cgaccatgaa caccatgaaa accaggctca tcatccgcgc catcgcctcg
     2581 ccacggaaca ggtcgcggac catcgccacg accagcaccc gggtgaccgc cgccgacgcg
     2641 ccctgcagcg cccgcgcggc gatcaacacg gtgaagctgc cggcgatccc gcacagcagg
     2701 gcgaacgccg cgtagagcgc gactccggcg gcgagcaccg gcttgcggcc gaagcggtcg
     2761 gcgaggggcc cccacaccag ctgggtcgag ccgaagccga tcatgtacgc gaccaccacc
     2821 agctggcggt ggttttcctg ctcgaccgac agcgattcgc cgatcgccgg aagcgccggg
     2881 atcatcgcgt cgatcgcgaa cgcgttgagc gccatcagcc cggcgagcaa agcggtcatc
     2941 tcgcggccgc cggggaggcg ctgaacggcc gccgcccgtg tcgatcggcc actggtcgag
     3001 gcgcggggcc tgttcaccgg gcgctgctgg ccgatcggcc gcgatcgtgc aacatgtctg
     3061 cgaacgggct tcgtggggct tgccaatgac gcatctgggt cgccggactt tcctgacggg
     3121 cagcgccgcc gcgagtgccg ctacgctgct gggcggcgcg agcttccatt cggcgcgggc
     3181 cgcggtcgcc gacccggcgc cgatcgtctt gccgccgccg atcggccgcg ccgagcggct
     3241 cgagcgcctc gccagggcgc gcacgctgat gaagcggcac ggcatcggcg cgatcctcat
     3301 cgagccgggc gcgagcctcg attatttcac cggggtccag tggtggcgct cggagcggct
     3361 gaccgccgcg gtgatcccgg ccgagggcga gccgatcgtc gtcacgccct tcttcgaaaa
     3421 gccgtcggtc gaggagagcc tggcgatccc cgccgaggtc cgggtctggc aggagcatga
     3481 ggaaccgctc aagctggtcg ccgatttcct gcgcgagcgc ggcgtcgccc gccagccggt
     3541 cggcttcgag gagaccaacc gcttcttcat cctcgaccgg ttgaaggagc agctgccgag
     3601 ggcgcagatc gtcagcgcca acccgatcgt ccgctccctg cgcatgatca agtcgccggc
     3661 cgagctcgcc ttgatgcagg cggcc
//
LOCUS       DASTZO010000217         6977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1167551,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000217 DASTZO010000000
VERSION     DASTZO010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1167551"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="VFU20_06110"
     CDS             <1..371
                     /locus_tag="VFU20_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019367616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5286069.1"
                     /translation="PALAGATDGLDRERRALAAFLRFAATNKQVYRIIDEAEFVDPQG
                     FRAHYETTAARIAARLSEAAGRGELRADESPLAHEVVAWALMGANVFLGLRFAVWGDS
                     DPDEVAAITNRLLRHGLAAR"
     gene            371..1258
                     /locus_tag="VFU20_06115"
     CDS             371..1258
                     /locus_tag="VFU20_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5286070.1"
                     /translation="MPNDSPPAQTAAPWQARTWTSRDGLELYFRDYPGSADRPPLLCL
                     HGLTRNSRDFEAFADRYTGRFRVIAVDFRGRGMSERDPQPARYLPVTYAADVLQLLDE
                     LGIERAVFVGTSLGGLVTMLVAGIQPQRIAGAILNDVGPELDERGLERIRSYVGKPMR
                     FANWDEAADYVAAINRNLPAAFTHDDWLRAARRLCREEGGAIVFDYDMAIAEPFNQPD
                     GAVAFDMWPLYRRLGQAPLLIVRGESSDLLSRATAKAMLDAAPGAQLVTVPGVGHAPE
                     LTEPAAVAAIDDFLGRFAG"
     gene            1336..1409
                     /locus_tag="VFU20_06120"
     tRNA            1336..1409
                     /locus_tag="VFU20_06120"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1368..1370,aa:Gly,seq:ccc)
     gene            1545..2093
                     /locus_tag="VFU20_06125"
     CDS             1545..2093
                     /locus_tag="VFU20_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286071.1"
                     /translation="MTRILALALAAAVALPLPIASTALAQPAFYAPARVDQMRAGPFW
                     VTVFEGTPPNSSNARNSLSRGIRVFEKPHRSTFAAVAAQEIFEFDFKARGGMAAQLNK
                     AQMEIISHEIESLVASRYGGYDLAAFEAKEAETLTRYARLRGMPAGEILARMRAARPI
                     AERWLAQNRDVIRALVAYRFPR"
     gene            complement(2107..2988)
                     /locus_tag="VFU20_06130"
     CDS             complement(2107..2988)
                     /locus_tag="VFU20_06130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286072.1"
                     /translation="MLLRLLPLAPLALASPVLAQSPLPAAEPSEIVVQGHRYRDEDVR
                     EFIDALTEAGGGDQISKFDWAVCPAAVGLSEPHRRAIAARMRQVAAAAGIPVAGGNCR
                     PNALVIATRDKQQLIEAMSRKYPAYFRDAHGQRTRPPRQPGPVTVWHVEGLLDANGIP
                     AGRDFDSKHFIVESMDSSRLIAASRPHFVAGILVVELNALAGLTNTQLADYAAMRLFA
                     RTDPSRLKKGAAPTILHAIDAPMGSAVPITLTNWDLAYLKALYSTGRNQIAHRQRHEM
                     QKKLRKELEGVRGRPER"
     gene            complement(3082..3840)
                     /locus_tag="VFU20_06135"
     CDS             complement(3082..3840)
                     /locus_tag="VFU20_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010234094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU5286073.1"
                     /translation="MAENKVWTAALVVIGDEILSGRTQDKNVAQVAAWLNEQGIRLAE
                     VRIVPDDAERIAETVNALRGAHDYLFTTGGIGPTHDDITVDSIAAALGVPVVVHPEAR
                     RILEDYYRSRGGLTEGRLRMARVPEGAELIPNPTSGAPGVRIGNIYLLAGVPHIAASM
                     LEALDGTLEGGRPMVSVTLGAFAAESDVAGLLREAEDRHPGVTVGSYPFLEEGRYGAN
                     FVMRSEDGDAARRCAGELGDALAAAGYEVVDGGI"
     gene            3891..4721
                     /gene="map"
                     /locus_tag="VFU20_06140"
     CDS             3891..4721
                     /gene="map"
                     /locus_tag="VFU20_06140"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006953022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU5286074.1"
                     /translation="MTEYITVSGDDRVEARSGVIKLHGPEGFEGMRAAGRLAASILDA
                     LVPHVVPGATTGEIDAIVHRLTLEGGGVPATLGYRGYTKSCCISINHVVCHGIPGDKA
                     LKDGDIVNVDVTPILDGWHGDTSRMYLVGDVALKARRLVDVTYECLMLGIEAARPGNR
                     LGDISSAIQRHAEAHRYSVVRDFCGHGLGRLFHDSPEVVHAGRPGTGPELKPGMFFTV
                     EPMINIGRADVKLLDDGWTAVTRDRSLSAQFEHSIGITETGCEIFTKSPKGLDKPPYA
                     "
     gene            complement(4861..5613)
                     /locus_tag="VFU20_06145"
     CDS             complement(4861..5613)
                     /locus_tag="VFU20_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEU5286075.1"
                     /translation="MKAIILSAGQGSRLGQLTAEKPKCLIDFGGRSLLDRQLDTLAAN
                     GVDEAVVVTGFRDDQVEAALARRAGGPRVRTIFNPFFKVADNLGSLFIARSELAGDCL
                     VWNGDTLVSNALMGRVVANRRPGICVTIDRKPSYDEDDMKVVTDGEGRLHAIGKRLPK
                     GNVNAESIGLLAFRDGGAERFSQAIDRAIRSAEGTTIWYLRVIHQIAQKDDVWTLDIQ
                     GEEWGEVDFPPDVAAARALVERWDRNQDVAAA"
     gene            5707..6045
                     /locus_tag="VFU20_06150"
     CDS             5707..6045
                     /locus_tag="VFU20_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEU5286076.1"
                     /translation="MKKIEAIIKPFKLDDVKDALHEVGVSGITVTEVKGFGRQKGHTE
                     LYRGAEYVIDFLPKVKVEVVVEDSLVDNTVEAIASAARTGRIGDGKIFVSDVEQAVRI
                     RTGDTGADAI"
     gene            6187..6453
                     /locus_tag="VFU20_06155"
     CDS             6187..6453
                     /locus_tag="VFU20_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286077.1"
                     /translation="MDDADTRTDSPTVDRATQTPRDERLASMSPDELRTAMRTLGYRT
                     QADLAGAIGVSRSAVSLWLEGKVGVPRPVAMLLRMLVAAQRRAF"
     gene            6575..>6977
                     /locus_tag="VFU20_06160"
     CDS             6575..>6977
                     /locus_tag="VFU20_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286078.1"
                     /translation="MNMLAKLAAVAALSTAAAIPSAAFAQAAPGRAIVSLYQAPPGHQ
                     EALMRWLAEQDRVAASVGVAPAQVYVHTDGAAWDFMVVQPLTTEAQDDAIEAAAKKMG
                     VASGPRAALEFRKHIQTHTDTFVRGPMTAAQF"
BASE COUNT         1037 a         2534 c         2333 g         1073 t
ORIGIN      
        1 caccggcgct cgccggagcc accgacgggc tcgaccgcga gcgccgggcg ctggccgcgt
       61 tcctgcgctt cgccgcgaca aacaagcagg tctaccggat catcgacgag gccgagttcg
      121 tcgacccgca gggcttccgc gcccattacg agacgaccgc ggcgcggatc gccgcgcgcc
      181 tgtcggaagc ggccgggcgc ggcgagctgc gcgccgacga atcgccgctc gcgcacgagg
      241 tcgtcgcctg ggcgctgatg ggcgccaacg tgttcctcgg cctgcgcttc gcggtgtggg
      301 gcgacagcga cccggacgag gtcgcggcga tcaccaaccg cctgctccgg cacgggctcg
      361 cggcccgctg atgccgaacg actctccccc cgcccagaca gcagcgccgt ggcaggcgcg
      421 cacctggacc agccgcgacg ggcttgagct ctattttcgc gactatccgg gcagcgccga
      481 ccggccgccg ttgctgtgcc tccacgggct gacccgcaac tcgcgcgatt tcgaggcctt
      541 cgccgaccgt tacaccggcc gcttccgggt gatcgccgtc gactttcgcg gccgtggcat
      601 gagcgagcgc gacccgcagc ccgcgcgcta cctcccggtc acctacgccg cggacgtcct
      661 gcagctgctc gacgagctcg gaatcgagcg cgcggtgttc gtcgggactt cgctcggcgg
      721 gctcgtcacc atgctggtcg ccggcatcca gccgcagcga atcgccgggg cgatcctcaa
      781 cgacgtcggc cccgagctcg acgagcgcgg cctcgagcgg atccgaagct atgtcggcaa
      841 gccgatgcgc tttgcgaatt gggacgaggc ggccgattat gtcgcggcga tcaaccgcaa
      901 cctgccggcg gcgttcaccc acgacgactg gctgcgcgcc gcgcggcgcc tgtgccgcga
      961 ggagggcggc gcgatcgtct tcgactatga catggcgatc gccgagccgt tcaaccagcc
     1021 ggacggcgcg gtcgccttcg acatgtggcc gctctaccgg cggctcggcc aggccccgct
     1081 gctgatcgtc cgcggcgaga gcagcgacct gctgtcgcgc gcgacggcca aggcgatgct
     1141 cgacgccgct cccggcgccc agctcgtcac cgttccgggc gtcggccacg cgccggagct
     1201 caccgagccg gcggcggtcg cggcgatcga cgatttcctc ggccggttcg ccggctgacc
     1261 cgtcgaggcc cggttgacgc tcgaagcccg gttgacgcca gaagcatcgt cgtggcaatc
     1321 ggcggctctc gccgggcggg tgtagctcaa tggcagagca gaagcttccc aagcttacga
     1381 cgagggttcg attcccttca cccgctccaa gattcgacat gccggggaca tgtcgaagct
     1441 ttggagcgcc cgcagcgcag cgaagcggcg acgccctgcc cttctgaacc cgagacaacg
     1501 agcgattcag acggccctca cccgccgggc gctatggccc tcgaatgacc aggattctcg
     1561 cacttgcact ggcggccgcc gtggcgctgc ccttgccgat cgcctcgacc gccctcgccc
     1621 aaccggcctt ttacgccccg gctcgcgtcg accagatgcg ggccggccct ttttgggtga
     1681 ccgtgttcga gggcactccg cccaactcgt cgaacgcccg caacagcctg tcgcggggca
     1741 tccgggtgtt cgagaagccg caccgctcga ccttcgccgc cgtcgccgcg caggagatct
     1801 tcgagttcga cttcaaggcc cgcggcggca tggccgccca gctcaacaag gcccagatgg
     1861 agatcatcag ccacgagatc gaaagcctcg tcgcgagccg ctacggcggc tacgacctcg
     1921 ccgcgttcga ggccaaggaa gccgagaccc tgacccgcta cgcgcggctt cgcggcatgc
     1981 cggcgggcga gatcctcgcg cgcatgcgcg ccgcccggcc gatcgccgag cgctggctgg
     2041 cgcagaaccg cgacgtcatc cgcgccctgg tcgcctaccg cttcccgcgc tagctcggcg
     2101 cttggcctag cgctcgggcc ggccgcggac cccttccagt tccttgcgca gcttcttctg
     2161 catctcgtgt cgttgccgat gggcgatttg attgcgcccg gtcgagtaca gcgccttcag
     2221 gtaagccagg tcccagttcg tcagggtgat cggcacggcg cttcccatcg gcgcatcgat
     2281 cgcgtgcagg atggttggcg cggccccctt tttcagccgc gacgggtcgg tccgggcgaa
     2341 cagccgcatg gccgcatagt ccgccagctg cgtatttgtc aggccggcga gcgcgttgag
     2401 ctcgacgacc aggattcccg ccacgaaatg cgggcgcgag gccgcgatca gccgcgagga
     2461 atccatgctt tcgacgatga aatgctttga atcgaagtcc ctcccggccg gaatcccgtt
     2521 ggcatcgagc aggccttcga catgccagac cgtcaccggc cccggctgcc tgggaggcct
     2581 ggtccgctgg ccgtgcgcgt ccctgaaata ggccggatac ttccggctca tcgcctcgat
     2641 cagctgctgc ttgtccctgg tcgcgatgac cagggcgttc ggccggcagt tcccgcccgc
     2701 gaccgggatt ccggccgccg cggcgacctg gcgcatgcgc gccgcgatcg cccgccggtg
     2761 cggctcgctg aggccgaccg cggccgggca gaccgcccag tcgaacttgc tgatctggtc
     2821 tcccccgccc gcctcggtga gagcgtcgat gaattcgcga acgtcctcgt cgcggtagcg
     2881 gtggccctgg acgacgatct ccgaaggctc cgcggcgggc agcggcgact gggcgaggac
     2941 cgggctcgcc aacgccaatg gtgcgagtgg cagcaagcga aggagcatgg ctcggctcct
     3001 ttgtaaccga ctgatgtggc ccggaatcta ccaccgccgg tgacgctcgg caacgaacgc
     3061 cccggcgagg gcgcgttccg ctcaaatgcc gccgtcgacc acctcgtagc cggctgccgc
     3121 cagcgcatcg ccgagctcgc cggcgcagcg gcgcgccgcg tccccgtctt cggaccgcat
     3181 gacgaaattg gccccgtagc ggccttcctc gaggaacggg tagctgccga cggtgactcc
     3241 cgggtggcgg tcctcggcct cgcgcagcag cccggcgacg tcgctttcgg cggcaaaggc
     3301 gccgagggtc accgagacca tcggccggcc gccctcgagc gtcccgtcga gcgcttccag
     3361 catgctcgcg gcgatgtgcg gcaccccggc gagcaggtag atgttgccga tccggactcc
     3421 cggcgcgccc gacgtggggt tggggatcag ctcggcgccc tccggcaccc gcgccatcct
     3481 gagccgcccc tcggtcaggc cgccgcggct gcggtaatag tcctcgagga tccggcgcgc
     3541 ctcggggtgg acgaccaccg gcacgcccag tgccgcggcg atcgaatcga ccgtgatgtc
     3601 gtcgtgcgtc gggccgatgc cgccggtcgt gaacagataa tcgtgggcgc cgcgaagcgc
     3661 gttgaccgtc tcggcgatcc gctcggcatc gtcgggcacg atccgcacct cggcgaggcg
     3721 gatgccctgc tcgttgagcc aggcagcgac ctgcgccacg ttcttgtcct gggtgcggcc
     3781 cgacaggatc tcgtcgccga tcaccaccag cgccgcggtc cagaccttgt tctcagccat
     3841 ccccgctccg aacggttggg tttgctttgc gctccgccta tatcgggccg atgaccgagt
     3901 atatcaccgt ctccggcgac gaccgggtcg aggcccgcag cggagtcatc aagctccacg
     3961 gccccgaggg cttcgaaggc atgcgcgccg ccggccgcct cgccgcctcg atcctcgacg
     4021 cgctggtccc gcacgtcgtt cccggggcga ccaccggcga gatcgatgcg atcgtccaca
     4081 gactgacgct cgagggcggc ggcgtcccgg cgacgctcgg ctaccgcggc tacacgaaga
     4141 gctgctgcat ctcgatcaac catgtcgtct gccacggcat ccccggcgac aaggcgctca
     4201 aggacggcga tatcgtcaac gtggacgtca ccccgatcct cgatggctgg cacggcgaca
     4261 ccagccgcat gtatctggtc ggcgacgtgg ccttgaaggc gcgccggctt gtcgacgtca
     4321 cctacgagtg cctgatgctc gggatcgagg ccgccaggcc cggcaaccgc ctcggcgaca
     4381 tttccagcgc catccagcgc catgccgagg cccaccgcta ctccgtcgtc cgcgatttct
     4441 gcggccacgg gctcggccgc ctgttccacg acagccccga ggtggtccac gccggccgcc
     4501 ccggcaccgg acccgagctc aagcccggca tgttcttcac ggtcgagccg atgatcaata
     4561 tcggccgcgc cgacgtaaag ctgctcgacg acggctggac cgccgtcacc cgcgaccgct
     4621 cattgagcgc ccagttcgag cattcgatcg gcatcaccga aaccggctgc gagattttca
     4681 cgaaaagccc gaaggggctc gacaagccgc cctacgctta agttcctccc ctgcatttgc
     4741 aggggagggg gaccatgcag cgcagactgc atggtggagg ggctggctgc ggtggtgaag
     4801 ggcccctcca ccagcttcgc tggttcccct cccctcgaaa tcgaggggag gaattagtcc
     4861 ttaagccgcg gcgacgtcct ggttgcggtc ccagcgctcg accagcgccc gcgccgccgc
     4921 gacgtcgggc gggaagtcga cctcgcccca ctcctcgccc tggatgtcga gcgtccagac
     4981 atcgtccttc tgggcgatct ggtggatcac ccgcagatac cagatggtcg tcccctcggc
     5041 ggagcggatg gcgcggtcga tggcctggct gaagcgctcg gcgccgccgt cgcggaacgc
     5101 cagcaggccg atcgattcgg cgttgacgtt gcccttgggc agccttttcc cgatcgcgtg
     5161 cagccggccc tcgccgtcgg tgacgacctt catgtcgtcc tcgtcgtaag atggcttgcg
     5221 gtcgatggtg acgcagatgc cgggccggcg gttggcgacc acccggccca tcagcgcgtt
     5281 ggagaccagg gtgtcgccgt tccacaccag gcagtcgccc gcgagctcgg agcgggcgat
     5341 gaacagcgag cccagattgt cggcgacctt gaagaacggg ttgaagatcg tccgcaccct
     5401 cgggcccccg gcgcgccgcg cgagcgccgc ctcgacctgg tcgtcgcgaa agccggtcac
     5461 caccaccgcc tcgtcgacgc cgttggcggc gagcgtgtcg agctggcggt cgagcaggct
     5521 gcggccgccg aagtcgatca ggcacttggg cttctcggcg gtcagctggc cgagccgcga
     5581 gccctggccg gcggagagga tgatcgcttt catgtcgctt gcgccaatgc ccgccaattg
     5641 cggcgcggat tgggcatcac taaggacgat tgcgccaatc cgggtggagc gcccaaggga
     5701 gtggacgtga aaaagatcga agcgatcatc aagccgttca agctcgacga cgtcaaggac
     5761 gcgctccacg aggtcggcgt cagcggaatc accgtcaccg aggtcaaggg cttcggccgc
     5821 cagaaaggcc ataccgagct ctaccgcggc gccgaatatg tgatcgactt cctgcccaag
     5881 gtgaaggtcg aggtggtggt cgaggacagc ctcgtcgaca acacggtcga ggcgatcgcc
     5941 agcgccgcgc gcaccggccg gatcggcgac ggcaagattt tcgtctccga tgtggagcag
     6001 gccgtccgga tccgcaccgg cgacaccggc gcggacgcga tctgacggct cccccggccc
     6061 gtcgttgcga ggagcgctag cgacgcggca atccagccgg cgccggactg gattgcttcg
     6121 cttcgctcgc aatgacgcag agccccttcc ccgtacgcgc gcgctccgtc taaagacctc
     6181 tttgcaatgg acgacgccga caccaggacc gactccccga ccgtcgaccg cgccacgcaa
     6241 acgccgcggg acgagcggct cgcctcgatg tcgcccgacg agctgcgcac cgcgatgcgg
     6301 accctgggct accggaccca ggccgacctc gccggagcga tcggggtcag ccgctcggcg
     6361 gtcagcctgt ggctcgaggg caaggtcggg gtgccgcgcc ccgtcgccat gctgctgcga
     6421 atgctcgtcg ccgcccagcg cagggcgttc tgacggccgc tcgccgcgtt tgactctcgc
     6481 cgcggccggg cgcaggccgg ggcccggcgc ggaagggggc tcgccgcgcc ggctgtaggc
     6541 ccccttagtc catgcaatct ttggggaact caccatgaac atgcttgcca agctggccgc
     6601 cgtcgcggcc ttgtccacgg ctgcggcgat tccgtcggcg gccttcgccc aggcggcgcc
     6661 gggccgcgcc atcgtctcgc tctaccaggc gcctccgggc caccaggagg cgctgatgcg
     6721 ctggctcgcc gagcaggacc gggtcgccgc ctcggtcggg gtcgctccgg cccaggtcta
     6781 tgtccacacc gacggcgctg cgtgggactt catggtcgtc cagccgctga cgaccgaggc
     6841 ccaggacgac gcgatcgaag ccgccgcgaa gaagatggga gtcgcgtcgg gcccgcgcgc
     6901 ggcgctcgag ttccgcaagc acatccagac ccacaccgac acctttgtcc gcggcccgat
     6961 gaccgcggcg cagttcc
//
LOCUS       DASTZO010000218         2694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_175254,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000218 DASTZO010000000
VERSION     DASTZO010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2694
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175254"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..999
                     /gene="ychF"
                     /locus_tag="VFU20_06165"
     CDS             <1..999
                     /gene="ychF"
                     /locus_tag="VFU20_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU5286079.1"
                     /translation="FCTIEPNVGRVAVPDERLDKIRAIAGSAQEIETQIEFVDIAGLV
                     RGASQGEGLGNKFLANIREVDAVVHVLRCFTGGDVTHVEGRVDPIADAEVVETELMLA
                     DLDSLDRRVPNLVKKAQQGDKEAKIEASVLGQALELLRDSKPARLVKPRDAEEQKALD
                     RAQLLTAKPVLYVCNVDEADAAEGNALSAQVFEKAAAEGAGAVVISAAIEAEIATLDE
                     TDRAAFLADLGLEETGLTRVIRAGYRLLGLITFFTAGPKEARAWTVPKGAKAPEAAGV
                     IHTDFERGFIRAETIAYDDFIACGGEAGAKDAGRMRAEGKDYVVQDGDVLLFRFNV"
     gene            complement(1006..1200)
                     /locus_tag="VFU20_06170"
     CDS             complement(1006..1200)
                     /locus_tag="VFU20_06170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286080.1"
                     /translation="MSTRKPFTLFAAAIFALMMLVHVYRLATDFQVIFGSHSVPMSVS
                     WIAIFVTGLLAVMLYRESQA"
     gene            complement(1197..1841)
                     /locus_tag="VFU20_06175"
     CDS             complement(1197..1841)
                     /locus_tag="VFU20_06175"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5286081.1"
                     /translation="MERTPSLLAAITTRERLVPLLAMATLAALWLAMLLLGAGEGDRS
                     VLLALYAGDEPWLALAALGFTYLGNWPTVVVVTILGAVWLLYRGKRRAALVLLVASFS
                     GRLLVILEKAYFARLRPEEDLRLAEVHYQSFPSGHAANSMIVYLAIALLAFDDPKHRR
                     RAAAGAVLLSVLIGLSRPMLGVHWPSDVVAGWAFGLFWTLLVLGLSWRWERRGQ"
     gene            1840..>2694
                     /locus_tag="VFU20_06180"
     CDS             1840..>2694
                     /locus_tag="VFU20_06180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5286082.1"
                     /translation="MDRAFNSRTAPNVAAALAHGRKLLADRPRLAAEQALEILASVPG
                     SADAYRLLGEALRRTGEDEAASDAELAAIAASVRDPELIRAGAALVDNDLPAAEAVLR
                     PLLRRRPTDVAAIRMMAELAARLGRRVDAENLLRRAIELAPGWAAARANLATILYRQH
                     RAEEALAELDHIERMGAEDDAQRNLRAAALGRLGGYDEAIALYRDVLSRHPGQPKVWM
                     SLGHLLKTVGEQAEAIAAYRRAIELVPSLGEVWWSLANLKTVRFSDEDVAAMQAALAE
                     PGLGDDDRL"
BASE COUNT          454 a          969 c          927 g          344 t
ORIGIN      
        1 ttctgcacca tcgagcccaa cgtcggccgc gtcgcggtgc ccgacgagcg gctcgacaag
       61 atccgggcga tcgccggctc cgcgcaggag atcgagaccc agatcgagtt cgtcgacatc
      121 gccggcctgg tccgcggcgc cagccagggc gagggcctcg gcaacaagtt cctcgccaac
      181 atccgcgagg tcgacgcggt cgtccacgtc ctgcgctgct tcaccggcgg cgacgttacc
      241 catgtcgaag ggcgggtcga cccgatcgcc gacgccgagg tcgtcgagac cgagctgatg
      301 ctcgccgacc tcgacagcct cgaccggcgc gtccccaacc tcgtcaagaa agcgcagcag
      361 ggcgacaagg aggcgaaaat cgaggcctcg gtgctcggcc aggccctcga gctattgcgg
      421 gactccaagc cggcgcggct ggtgaagccc agggatgccg aggagcagaa ggcgctcgat
      481 cgcgcgcagc tgctgaccgc caagccggtg ctctacgtgt gcaacgtcga cgaggccgat
      541 gccgccgagg gcaacgctct gtcggcgcag gtgttcgaga aggccgcggc ggaaggcgcg
      601 ggcgcggtgg tgatctcggc cgcgatcgag gcggagatcg cgaccctcga cgaaaccgac
      661 cgggcggcgt tcctcgccga tctcggcctg gaggaaaccg gcctcacccg ggtgatccgc
      721 gccggctacc gattgctcgg cctgatcacc ttcttcaccg ccgggcccaa ggaggcgcgc
      781 gcctggacgg tgcccaaagg agcgaaagcg ccggaggcgg ccggagtcat ccacaccgac
      841 ttcgagcgcg gcttcatccg cgccgagacc atcgcctacg acgacttcat cgcctgcggc
      901 ggcgaggcgg gcgccaagga cgccggcagg atgcgcgccg agggcaagga ctatgtcgtc
      961 caggacggcg acgtcctgct gttccgcttc aacgtctagg agcgcttacg cctggctctc
     1021 ccggtacagc atcaccgcca gcaagccggt gacgaagatg gcgatccagc tcaccgacat
     1081 cgggaccgaa tggcttccga agatgacctg gaagtcggtc gcgagccgat agacgtggac
     1141 cagcatcatc agcgcgaaga tcgccgccgc gaacagagtg aagggtttgc gagtgctcat
     1201 tggcccctcc tttcccatcg ccagcttaga cccaggacca gcagcgtcca gaacaatccg
     1261 aacgcccagc cggcgaccac gtcgctcggc cagtggacgc cgagcatcgg ccggctcaac
     1321 ccgatcagga cgctgagcag cacggccccc gcggccgcgc ggcggcggtg cttcgggtca
     1381 tcgaaggcga gcaaggcgat cgccaggtag acgatcatcg aattggccgc gtggccgctc
     1441 gggaacgact ggtaatggac ctcggcgagg cgcaaatcct cctcgggccg caggcgggcg
     1501 aaataggcct tttcgaggat caccagcagc cgcccggaga aactcgcgac cagcagcacc
     1561 agcgccgccc ggcgcttgcc gcggtacagc agccagaccg cgccgaggat cgtcaccacg
     1621 accaccgtcg gccaattgcc gagataggtg aaaccgagcg ccgcgagcgc cagccacggc
     1681 tcgtcgcccg cgtaaagcgc gagcagcacc gaccggtcgc cctcgccggc gccgagcagc
     1741 agcatcgcca gccacagcgc ggcgagcgtg gccatcgcca gcagcggcac cagccgctcc
     1801 cttgttgtga tcgccgctaa gaggctaggc gttcgttcca tggacagggc gttcaattcc
     1861 cggaccgctc caaatgttgc cgcggcgctc gcccacggcc gcaaattgct cgccgaccgg
     1921 ccgcggctcg cggccgagca ggcgctcgag atcctcgctt cggtccccgg cagcgccgac
     1981 gcctaccggc tgctcggtga ggcgcttcgc cgcaccggcg aggacgaggc cgccagcgac
     2041 gccgagctcg cggcgatcgc cgcgtcggtc cgcgaccccg agctgatccg cgccggcgcg
     2101 gcgctggtcg acaacgacct cccggccgcc gaggcggtgc ttcggccgct gctcaggcgc
     2161 cggccgaccg acgtcgcggc gatccgcatg atggccgagc tcgccgcacg gctcggccgc
     2221 cgggtcgacg ccgagaattt gctccgccgg gcgatcgagc tcgcgcctgg ctgggcggcg
     2281 gcgcgcgcca acctcgccac gatcctctac cgccagcacc gggccgagga agcgctggcc
     2341 gagctcgacc atatcgagcg catgggggcg gaggacgacg cccagcgcaa tttgcgcgcc
     2401 gcggcgctcg ggcggctcgg cggctacgac gaggcgatcg ccctctaccg cgacgtcctg
     2461 tcgcgccacc cgggccagcc gaaggtgtgg atgagcctcg gccatctgct caagacggtc
     2521 ggcgagcagg ccgaggcgat cgccgcctac cgccgggcga tcgagctcgt tccgagcctc
     2581 ggcgaggtct ggtggagcct cgccaacctg aagaccgtgc gcttctcgga cgaggacgtc
     2641 gcggcgatgc aggctgcgct ggccgagccc gggctcggcg acgacgaccg gctg
//
LOCUS       DASTZO010000219         2084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1037160,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000219 DASTZO010000000
VERSION     DASTZO010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037160"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFU20_06185"
     CDS             <1..508
                     /locus_tag="VFU20_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5286083.1"
                     /translation="AEYLEFITVVNRNESGATIPAIEVDMADFREVQNTPKWTMSGSL
                     DYDTPVGDGRLNANATLSYRSKSQQFELAIPQLDQKGFALLDASIVWRSAGNRYTIGL
                     HGKNLTDKRYVTAGYNFLRQNPYTGEFILNNAAGTPGLNSTLGTEGILTAYYGAPRQV
                     FLSFGVKF"
     gene            556..1788
                     /locus_tag="VFU20_06190"
     CDS             556..1788
                     /locus_tag="VFU20_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5286084.1"
                     /translation="MLLLLLLAYIFNFVDRQIIGVLAVPIKAELELSDRQLGLMGGVA
                     FAIFYSTLAIPIAWLADRKSRIAIVSASVAFWSLFTALCGFAQNFWQLFVARMGVGIG
                     EAGGVAPSYSLIADYFPPASRARALAVFSFGIPIGSALGIYFGGWIASSVDWRTAFIV
                     VGLAGIPAAVLLKLFVPEPKRGATDAILSDDAPPFLDVARLLARNPSFWLLSLGAASG
                     SIMGYGLIFWLPSFFARSFGLGLNDIGWFYGSIVLVGGMLGIWLGGVFGDRLGAARPG
                     AYALIPALCFLAAAPLFAAGLFAPSLGVAWLLFVVPQMLALAWLGPVLAAIQRLVPSN
                     MRSTASASFLLINNLIGIGLGVWIIGEMSTRMEAAYGDESLRWSVLYGLGFYLLSALF
                     YLIAARRLSGDVYEAPAR"
     gene            1829..>2084
                     /locus_tag="VFU20_06195"
     CDS             1829..>2084
                     /locus_tag="VFU20_06195"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF021908.4"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HEU5286085.1"
                     /translation="MTRSDDIPAILDQLCEIYDSSAAALRAALAAYVSSGTRPDAAAR
                     ADGCFAYPELRIDYEPAAEPPAPARAFARLNQPGIYASSIA"
BASE COUNT          281 a          733 c          674 g          396 t
ORIGIN      
        1 cgccgaatat ctcgagttca tcacggtcgt gaatcgcaac gaatcgggcg cgacgatccc
       61 ggcgatcgaa gtcgacatgg ccgacttccg cgaggtccag aacacgccca aatggacgat
      121 gagcggcagc ctcgactatg acactccggt cggcgacggg cgattgaacg ccaacgcgac
      181 cctgtcctac cggagcaaga gccagcagtt cgagctcgcc atcccgcagc tcgaccagaa
      241 gggcttcgcc ctgctcgacg cgagcatcgt ctggcgctcg gccgggaacc gctacacgat
      301 cggcctgcac ggcaagaatt tgaccgacaa gcgctacgtc acggccggct acaatttcct
      361 tcggcagaac ccctacaccg gcgagttcat cctcaataac gccgccggca ctccgggact
      421 gaactcgacg ctcgggaccg agggcatcct gaccgcttat tacggcgcgc cgaggcaggt
      481 gttcctgtcg ttcggcgtga aattctgagc tctccggcgg gggccgaccc gggagcacgc
      541 gcgcttcgaa cgcgcgtgct cctgcttttg ctgctggcct acatattcaa cttcgtcgac
      601 cgccagatca tcggcgtgct ggcggtgccg atcaaggccg agctcgagct gtccgaccgc
      661 caattggggc tgatgggcgg ggtcgccttc gccatcttct attcgaccct ggccatcccg
      721 atcgcctggc tcgccgaccg caagagccgg attgcgattg tctccgcgtc ggtcgccttc
      781 tggagcctgt tcaccgcttt gtgcggcttc gcccagaatt tctggcaatt gtttgtcgcc
      841 cggatggggg tcggcatcgg cgaggccggc ggggtggccc ccagctattc gttgatcgcc
      901 gattatttcc cgccggccag ccgcgcccgg gcgctggccg tattctcgtt cgggatcccg
      961 atcggatcgg cgctcggcat ctatttcggc ggctggatcg cgagcagcgt cgactggcgg
     1021 accgccttca tcgtcgtcgg cctcgccggg atcccggcgg cggtcctgtt gaagctgttc
     1081 gtccccgagc cgaagcgcgg agcgaccgac gcgatcctgt ccgacgacgc gccgccgttc
     1141 ctcgacgtcg ccaggctgct cgcccgcaac ccgagcttct ggctcttgtc gctcggcgcc
     1201 gcgtcgggat cgatcatggg ctacggcctg atcttctggc tgccgagctt cttcgcccgc
     1261 agcttcgggc tcggcctcaa cgacatcggc tggttctacg gctcgatcgt gctggtcggc
     1321 gggatgctcg gaatctggct gggcggggtg ttcggcgacc gcctcggcgc ggcccggccc
     1381 ggcgcctacg cgctgatccc ggcgctgtgc ttcctcgccg ccgcgccctt gttcgcggcc
     1441 ggcctgttcg ctccgtcgct cggggtcgcc tggctgctgt tcgtggtccc gcagatgctc
     1501 gccctggcgt ggctcgggcc ggtgctggcg gcgatccagc ggctggtgcc ctcgaacatg
     1561 cgctcgaccg cctcggcctc gttcctgctg atcaacaatc tgatcggcat cgggctgggc
     1621 gtgtggatca tcggcgagat gtcgacccgg atggaggccg cctacggcga cgaatcgctg
     1681 cgctggtcgg tgctctacgg cctcggcttc tacctgttgt cggcattgtt ctacctgatc
     1741 gcggcgcgcc ggctttcggg cgacgtctac gaggctccgg cgcgctgaac attgccagcg
     1801 tcggcgtggc cgcctagcga gagtcggaat gacgcgatcc gacgacattc ccgcgatcct
     1861 cgaccagctt tgcgagatct atgattcctc cgccgcggcg ctgcgcgcgg cgctcgccgc
     1921 ttatgtcagc agcggcaccc gccccgacgc ggcggcgcgc gccgacggct gcttcgccta
     1981 ccctgagctg cgcatcgact atgagcccgc cgccgagccg ccggccccgg cgcgggcgtt
     2041 cgcgcgcctg aaccagccgg gaatctacgc gtcgagcatc gccc
//
LOCUS       DASTZO010000220         6381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_800334,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000220 DASTZO010000000
VERSION     DASTZO010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800334"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..1534
                     /locus_tag="VFU20_06200"
     CDS             224..1534
                     /locus_tag="VFU20_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286086.1"
                     /translation="MDHSTMGHGAVPAPAEPAPAAPVDHSQMDHATADPKAMAADHGG
                     HADLTGALGPYPMTRESSGTAWQPDNSGHMGLMAASGDWSFMAHGILNLVYDHQSGRR
                     GDDMVFPSGMLMAMARRPLGDGTLQVRASLSPDPLMGKRGYPLLLASGETANGTDRLI
                     DRQHPHDFFMELSASASQAIGPKSSVFLYAALPGEPAFGPPALMHREAILDSPEAPIS
                     HHWLDSTHISFGVLTGGLVLDRVKVEVSRFNGREPDQHRWNIETAPLDSTAARLSWNP
                     TRSLSLQGSWGYFEEPEQLEPGVDQRRWSASVLYAREIAPDWKLAATLAWGRKSIQDD
                     HGDRLNDDAFVAEASLKHRSWTLFGRAELTENRELVDELEHGPAFEVGKASLGALRDF
                     RVADHLSLGVGGLLAVNFVPDGLAPLYGGRHPMGAMGFIRLKLD"
     gene            1537..2184
                     /locus_tag="VFU20_06205"
     CDS             1537..2184
                     /locus_tag="VFU20_06205"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEU5286087.1"
                     /translation="MNGRFFTMLVVAVALAAAPAGAHKKKHDEHAAPSNVATQPASGA
                     TLDSATSSPMPAPSHSSRSEPMGEMGSDRASMSFVARMLDWLGRLHPVIVHFPIAFFP
                     AALFTAVAGRRRPAFGKPVQFLVVAGGILAPVAAILGWLDAANADPGPLLTVHRWLGT
                     AVGVGGSGLAIWAARKPDQSRSSGMIIALAVITGAIVVQGWYGGALVHGIDHMDW"
     gene            2204..2695
                     /locus_tag="VFU20_06210"
     CDS             2204..2695
                     /locus_tag="VFU20_06210"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5286088.1"
                     /translation="MPTIAIRSAVLAALLASASPTSAREPVAKAIQASSLSSSARSAA
                     SAVDAFHAALRRGDTRSAAALLATDALIFEGGGVERSRAEYASHHLAADAAYSQAVPA
                     VVTRRSGDSAGSVAWIASEGRTTGTFKGKAVNRVTAETMVLRRIGRSWKIVHIHWSSA
                     AKP"
     gene            2879..2954
                     /locus_tag="VFU20_06215"
     tRNA            2879..2954
                     /locus_tag="VFU20_06215"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2913..2915,aa:Glu,seq:ctc)
     gene            3583..>6381
                     /locus_tag="VFU20_06220"
     CDS             3583..>6381
                     /locus_tag="VFU20_06220"
                     /inference="COORDINATES: protein motif:HMM:NF039867.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5801 repeats-in-toxin domain-containing
                     protein"
                     /protein_id="HEU5286089.1"
                     /translation="MRYEDASGSFSSQGTEAPDARDDADALPAGSRGPATGNAITGEG
                     TITGAAGADVVTDGPGKIVALEGAGGGDSADGGSLHVEGRFGELTMDSRGGYSYSPHA
                     AKPEGGSDVFRYTLADADGDRDTATLTIDIGREIEKIEANAQQIIPGPDGVVTLPPGV
                     ELSDILVVGRNLVINLPDGTQMVIIDGAVFVPQLVLGGVEVPASNLAALLIDAEPKPA
                     AGTPLSSGGNFAVDVPPLDPGVPLGDLIPPTELVFTPPIFEEAGQIADDEPEILIQPD
                     GQPAVVNAIDSVNEAGLPTRNSGEPAGSGESADGNGADNDDPSEATTGVILIDAKDDP
                     AVVTINGVAVTGVGQVIAGAHGNLTITSISDEAIGYSYVLRDNTSGDATHDDFSVTVT
                     DRDGDQATATLTINIVDDVPTARPDTDSIAAGTFGPATGNLITDAAAGDTGDSDSGAD
                     TVGADNATLTSISGAGGSDSTFSSGVLTVNGQYGVLTVEADGDYSYVRNPNTPGGVTD
                     TFNYTITDGDGDTSSSTLTITIADRLVTVGTNPLVRTDDDAVPLAGGNPGGTGDDVDA
                     ENLTGTLSGSGGDGTLTFDLLTTGAPAGFTYVDGPGGSVLVQQGGVTVLTITVNATTG
                     AYTVTQNAPIDHPALGDENNLEFTINYTVTDLDGDSAAGTLTINVDDDTPTITRQDIS
                     APTLTVDETDLATNATGNFSTLFAVSYGADGAGTTSYSVSVVNGTDSGLDDVATGQDI
                     LLFNNGGVVEGRVGSATGALAFTVSVDSATGVVKLDQIRALEHPDATNPDDSVSPTAT
                     SIQFTVNAVDGDGDPVSQTINIGSNLVFDDDGPSLTNVAAGAGVLLDETTAGTPAGFP
                     ISGTSASAAITATQAFGADGAGSVVYGLSLAGGGSSLASGLQTAIGDHAITLVQVDAD
                     TIEGQYNGTNVAFTIQMNA"
BASE COUNT         1122 a         2235 c         2059 g          965 t
ORIGIN      
        1 gcccgcccag cacgccggcc accacggagc gccgccgccc gccgcgaaaa aggcgccggc
       61 aaaaaagaag ccggccgcca agaagcccgc ggcgaagaag gcgcccgcca ggaagcccgc
      121 ggccacgaaa gcgccggcca agaaggcccc cgccacgaaa accccggccg tggagcccgc
      181 agcgcccgcc gcgccgggcg cgccggttcc agaccattcg cagatggacc attcgactat
      241 gggccacgga gcggtgccgg ctccggccga gcccgcgccg gccgcgccgg tcgaccattc
      301 gcaaatggat catgcgaccg cggatcccaa ggcaatggct gccgatcatg gcggccacgc
      361 cgacctgacc ggggcgctcg ggccgtaccc gatgacccgc gaatcgtccg gcacggcctg
      421 gcagcccgac aattccgggc acatgggcct gatggccgcc agcggggact ggagcttcat
      481 ggcccacggt atcctcaacc tcgtctacga tcaccagtcg ggccgccgcg gcgacgacat
      541 ggttttcccg tcgggcatgc tgatggccat ggcccggcgg ccgctcggcg acgggacgct
      601 gcaggtccgg gcctcgctca gccccgaccc gctgatgggc aagcgcggct acccgctgtt
      661 gctcgccagc ggcgaaaccg ccaacggcac cgaccggttg atcgaccgcc agcacccgca
      721 cgatttcttc atggagctgt ccgcctcggc gtcgcaggcc atcgggccga agagcagcgt
      781 cttcctgtac gcggcgctgc cgggcgagcc ggcgttcggg ccgccggctc tcatgcatcg
      841 cgaggcgatc ctcgattcgc ccgaagcgcc gatcagccac cactggctcg attcgaccca
      901 catcagcttc ggggtgctga ccggcgggct ggtgctcgac cgggtgaagg tggaggtcag
      961 ccgcttcaac ggccgcgagc ccgaccagca ccgctggaac atcgagacgg cgccgctcga
     1021 ctcgaccgcg gcgcggctgt cgtggaatcc cactcgaagc ctctcgctcc aagggagctg
     1081 ggggtatttc gaggagcccg agcagctcga gcccggggtc gaccagaggc gctggtcggc
     1141 gagcgtgctt tatgcgcgcg agatcgctcc ggactggaag ctcgcggcga cgctcgcctg
     1201 ggggcgcaag tcgatccagg acgaccacgg cgaccggctg aacgacgacg ccttcgttgc
     1261 cgaagcgtcg ctcaagcacc gctcctggac gctgttcggg cgcgccgagc tgaccgagaa
     1321 tcgcgagctt gtcgacgagc tcgagcacgg cccagccttc gaggtgggca aggcgtcgct
     1381 cggtgcgctg cgcgacttca gggtcgccga ccatctgtcg ctgggcgtcg gcggcctgct
     1441 cgccgtgaat ttcgtgcccg atggcctcgc tccgctgtat ggcggccgtc acccgatggg
     1501 cgcgatgggt ttcattcgcc tgaagctcga ttagccatga atggtcgctt ctttacaatg
     1561 ctcgtggtcg cggtggcctt ggctgccgct cccgccggtg cgcacaagaa aaagcatgac
     1621 gagcacgccg cgccatcgaa tgtggcaacg cagccggcaa gcggtgccac cctcgattcc
     1681 gcgaccagca gcccgatgcc tgcgcccagc cattcatcga ggtcggaacc gatgggcgaa
     1741 atgggctcgg acagggcatc gatgtcattc gtcgcgcgca tgctcgactg gctcgggcgg
     1801 cttcacccgg tcatcgtgca tttccccatc gccttcttcc cggcggcgct tttcaccgcg
     1861 gtcgccgggc gccggcggcc ggcctttggc aagcccgttc agttcctggt ggtcgccggc
     1921 ggcatcctcg cgcccgtcgc cgcgatcctt ggctggctgg acgccgccaa cgccgacccc
     1981 ggtccgctcc tcacagtcca ccgctggctc ggaacggcgg tgggcgtcgg gggatcggga
     2041 cttgcgatct gggccgcccg gaagccggac caaagccgca gctctggaat gatcatcgcg
     2101 ctggccgtga tcactggcgc gatcgtcgtc cagggctggt acggaggcgc gctcgttcac
     2161 ggtatcgatc acatggactg gtaacgggat ccgggagact gacatgccga ccattgcaat
     2221 tcgtagtgca gtgcttgccg cccttttggc gtcggcgtcg ccaacatccg cccgcgagcc
     2281 agtcgcgaag gcgatccagg caagctcgct gtcgtcatcc gcacggagcg cggcatcggc
     2341 agtggatgcg tttcacgcag ccttgcgccg gggcgacaca cgatccgcgg cagcattgct
     2401 cgccaccgac gcgctgatat tcgagggcgg cggggtcgag cgcagcaggg cggaatatgc
     2461 ctcgcatcat cttgccgcgg atgcggccta ctcccaggca gtcccagccg tggtgacgcg
     2521 ccggtccggc gattctgccg ggtcggtcgc ctggatcgca agcgagggcc gcaccaccgg
     2581 tacattcaag ggcaaggcgg tgaatcgggt tacagccgaa acgatggtgc tgcgccgaat
     2641 cggccgctcg tggaaaatcg tccacatcca ttggtcgtcg gctgcaaagc cctgatcggc
     2701 cctccgccgc ctctgaacag cggcctgagt tttccgcgat ggatacttcc gcgccgtccg
     2761 gcaggcgcgg ttcggcaagt gcatgggctg ccgcgacggc aaagcgcgcc acgaccgcca
     2821 gtggcgactg attgactagg ctagcccgag cccacatatg gccggctctc aagccgacgg
     2881 ccccttcgtc tagcggtcca ggacgtcgcc ctctcacggc gaaaacacgg gttcgagtcc
     2941 cgtaggggtc accaatcgcg gccggctttc gaggctcgaa gaacggttga agtgccgcag
     3001 cttgcgactc ggtcgttcga acggctcgca tgcggccttg cgactccgag tcgcggcgcc
     3061 cccgaccggt ccgaaggtcg aaaagtccca ttccgggacg catccattca gatttttcgt
     3121 gagcggccaa cactttggcg gttcgcccga gctcgcccaa caacgccgct attaaacccg
     3181 cgttaaacgg aagctaacca aatctgctaa agggctatgc tgcgaaagta gagcaaataa
     3241 gaatagagct gttggatgaa tactggcagg tcgctaggaa acgcccgaat aaactataaa
     3301 gatgttactc gctttgataa gttatgcttg gccatggatg tgtattactg aacacgttaa
     3361 ccaatatata gcctcgtcac gattctgtaa tgttgtccag gccgcgcgtg gtttatgccc
     3421 gggagcttgc caaatgcgta agtcggtgtc ttgtgttgag ctacgcgggg tgccgactct
     3481 cgaccgttaa ccatgcgcgt tacgctggtc ggtcgagccg agggggcctc ggcaacagct
     3541 caagcggcgg gccgcggcgg cgggactaaa caggggtggt ttatgcgata cgaagatgct
     3601 tcgggctcct tcagcagcca ggggacggaa gctcccgacg ctcgtgacga tgccgatgca
     3661 ctgccggccg gcagccgcgg tccggcgacc ggcaacgcca tcaccggcga aggcaccatc
     3721 accggagccg ccggcgccga cgtcgtcacc gacgggccgg gcaagatcgt cgccctcgag
     3781 ggcgccggcg gaggcgactc cgcggacggc ggcagccttc acgtcgaggg ccgcttcggc
     3841 gagctcacca tggatagccg cggcggctac agctactcgc ctcacgccgc caagcccgaa
     3901 ggcggcagcg acgtgtttcg ctacacgctc gcggatgccg acggcgaccg cgacaccgcg
     3961 accctgacca tcgacatcgg ccgcgagatc gagaagatcg aggccaacgc gcagcagatc
     4021 atcccgggac ccgacggagt cgtcacgctt ccccccggcg tcgagctctc cgacatcctg
     4081 gtcgtcggcc gcaacctggt catcaacctg cccgacggca cccagatggt catcatcgac
     4141 ggcgccgtgt tcgtgcccca gctggtgctc ggcggagtcg aggtcccggc aagcaacctg
     4201 gcggcgcttc tgatcgacgc ggagcccaag cccgcggccg gcaccccgtt gagcagcggc
     4261 ggcaatttcg cggtcgacgt gccgccgctg gatcccggag ttcccctcgg cgacctgatt
     4321 ccgccgaccg agctcgtctt cactccgccg atcttcgagg aagccggcca gatcgccgac
     4381 gacgagcccg aaatcctgat tcagcccgat ggccagccgg ccgtggtcaa tgccatcgac
     4441 agcgtcaacg aagctgggct tccgacgcgc aattccggcg agcctgccgg tagcggcgag
     4501 tcggccgatg gcaacggcgc cgacaacgac gatcccagcg aggcgacgac cggggtcatc
     4561 ctgatcgatg cgaaagacga ccccgcggtc gtgacgatca atggagtcgc ggtgaccggc
     4621 gtcggccagg tgatcgcggg agcccacggc aatctgacca tcaccagcat cagcgatgaa
     4681 gcgatcggct acagttatgt gctgcgcgac aatacgtccg gcgatgcgac ccacgacgat
     4741 ttctcggtca cggtcaccga ccgcgacggc gaccaggcga cggcgaccct gacgatcaac
     4801 atcgtcgacg acgtcccgac cgctcgtccc gacaccgaca gcatcgcggc gggcacgttc
     4861 ggcccggcga ccggcaacct catcaccgac gcggcggccg gcgacaccgg cgacagcgac
     4921 agcggcgcgg acacggtcgg cgccgacaac gcgaccctga cctcgatcag cggcgcgggc
     4981 ggcagcgatt cgacgttcag ctccggggtg ctgacggtca acggccagta tggcgtcctg
     5041 accgtcgagg ccgacggcga ctacagctac gtgcgcaacc cgaacacgcc gggcggagtc
     5101 accgacacct tcaattacac gatcaccgac ggcgacggcg acacgtcgag ctcgaccctg
     5161 accatcacca tcgccgaccg cctcgtgacg gtcggcacca acccgctcgt gcgcaccgat
     5221 gacgacgcgg tcccgcttgc gggcggcaat ccgggcggca ccggcgacga tgtcgatgcc
     5281 gagaatttga ccggcactct gagcggcagc ggcggcgacg gcacgctgac cttcgacctc
     5341 ttgacgaccg gcgcgcccgc cggcttcact tatgtcgacg gccccggcgg ctcggtgctc
     5401 gttcagcagg gcggggtcac cgtcctcacc atcaccgtca acgccaccac cggcgcctac
     5461 acggtgactc agaacgcgcc gatcgaccat ccggcgctcg gcgacgagaa caacctagag
     5521 ttcacgatca actataccgt caccgacctc gacggcgatt ccgcggcggg cacgctgacg
     5581 atcaacgtcg acgacgacac gcccaccatc actcgccagg acatctccgc cccgaccctg
     5641 acggtcgacg agaccgacct ggcgacgaac gccaccggca acttctcgac cctgttcgcg
     5701 gtcagctacg gcgccgacgg cgccggcacg accagctatt cggtcagcgt cgtcaacggc
     5761 accgacagcg gcctcgacga tgtcgcgacc ggccaggaca tcctgctttt caacaacggc
     5821 ggggtcgtcg agggccgcgt cggaagcgct accggcgcac tcgccttcac tgtcagcgtc
     5881 gattcggcga ccggagtggt caagctcgac cagatccgcg cgctcgagca ccccgacgcg
     5941 accaatcccg atgattcggt gagcccgacg gcgacgtcga tccagttcac cgtcaatgcg
     6001 gtcgacggcg acggcgaccc ggtcagccag acgatcaaca tcggctccaa cctggtattc
     6061 gatgacgatg gcccgagcct gaccaacgtc gcggccgggg ccggggtcct gctcgacgag
     6121 accacggccg ggacgccggc cggtttcccg atcagcggca ccagtgcgtc cgcggccatc
     6181 accgcgaccc aggcgttcgg cgccgacggc gccggctcgg ttgtctatgg attgtcgctg
     6241 gccggcggcg gttcgtcgct cgcaagcggc cttcagaccg cgatcggcga ccacgccatc
     6301 accctcgtgc aggtcgacgc cgacacgatc gagggccagt acaacggcac caacgtcgcc
     6361 ttcaccatcc agatgaacgc g
//
LOCUS       DASTZO010000221         5336 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_645366,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000221 DASTZO010000000
VERSION     DASTZO010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5336)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5336)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5336
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645366"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..647
                     /locus_tag="VFU20_06225"
     CDS             153..647
                     /locus_tag="VFU20_06225"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HEU5286090.1"
                     /translation="MADHSEMNKPARSRARPLSELLGKTLGDAFARQGFASTGFVTRW
                     ADIVGPEIAAHSQPEKIQWPPPRDGQTAEPGTLVLRVEGPTALEIQHLSRVILERVNR
                     FFGWQAIAAIRLRQAPLRRDRRRPPPGPDPAATEALARSLPEIEDEKLRQALARLGAA
                     IKQP"
     gene            794..1438
                     /locus_tag="VFU20_06230"
     CDS             794..1438
                     /locus_tag="VFU20_06230"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEU5286091.1"
                     /translation="MIAAAAGGGYYAWSQSRSPATPAPTAPGATLPSGAAAELMEPGP
                     LGEQAVGNANAPVTIIEYASMTCPHCAQFHEQVYPELKKKYVDTGKVRFIFREFPLDP
                     LAAAGFMLARCGDKDKYFPMIETLFAQQKDWVVQKPLEPLLAIAKQAGFTQESFDKCL
                     ANQKVLDGIEEVRTRAAQKFQVTSTPTFFVNGKMLRGSPTLEELSKQIEANLKS"
     gene            1660..5118
                     /locus_tag="VFU20_06235"
     CDS             1660..5118
                     /locus_tag="VFU20_06235"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5286092.1"
                     /translation="MKLTRLRLLGFKSFVEPTDFLIEPGLTGVVGPNGCGKSNLVEAL
                     RWVMGETSHKSLRAADMEDVIFAGTNNRPGRNNAEVAMHIDNSDRKAPAQFNDTESLD
                     VSRRIEREKGSSYRINGREVRARDVQILFADASTGSRSPALVHQGRIGEIIQAKPEQR
                     RRVLEEAAGISGLHARRHEAELRLRAAEQNLLRLEDVINQLNAQMASLKTQARQAVRY
                     RNIAGQIRRTEATLFHLRWTAASAELAAAEQAMNEAVRVVADRTGAQAEAATRQALAA
                     AELPALREAEAAAAAGLHRLVVARETLEKEEARARDRIAELDRRLVQLEGDIERERQH
                     VVDAEAALTRLAAEEESLKQDIDANAEKRTGADARVAEAGAVLGVAEKTFGELTGTLA
                     DLTARRKALEQAARAHDDRLARLANETAAVEAELASLEAANAGHPDVAALAIAAEEAQ
                     AAVTEAEAASLRAEAAHSAARQALDVARGPLAEAERRAGRLDTEAKTLAKLLHVDTKN
                     LWPAVIDDLKVEKGYEAALGAALGDDLEAPVDPSAPMRWAGAEIDPSDPVLPDGAEPL
                     GAYVSAPKEMARRLAQIGVIVREDALRLMPALKPGQRLVSRDGDLWRWDGFSVAANAP
                     TGAARRLAGKNRLADIETELSTVRHDVEARQQAAAHAQAEVTAAAAAETAARNRWREL
                     QQAAHAARNRHAEVEREVSRNSARRSALGEAKARLTASRDEAAIARGDVEQSLTALPP
                     SADIEERLAAVKTEIEGHRTRVAEVRAEAQGLAREAEMAARRLVTIGSERESWTARNA
                     GATSQLETLSVRVEEAKAERADLENAPQVFAEQRTALIGEIETAEGNRRAAADRLAEA
                     ETRLAEADRDARAALDAMGSAREDAARAEERFEAGKRRLADIDHEIVEMLEVAPAEVA
                     GLAELNPDKPLPEVAEVESTLERLRRERERLGAVNLRADEELREVETQHTTLTTERDD
                     LVEAIKRLRQGIQSLNREARERLLASFEVVNNHFRELFTHLFGGGTAELKLIEAEDPL
                     EAGLEIIAKPPGKKPATLSLLSGGEQALTALALIFAVFRTNPAPICVLDEVDAPLDDH
                     NIERFCDLLDEMTRATDTRFVIITHNPITMARMNRLFGVTMAERGISQLVSVDLEGAV
                     RLREAS"
BASE COUNT          930 a         1831 c         1838 g          737 t
ORIGIN      
        1 ccgcgcgcca tggcaatacg cgccggtggc ggtcgtacca gtcgaggagc gatgccggat
       61 tgggtgaggc tccgggtccc cgttttcgcg gggacgagcg gaggccggat cgagccttcc
      121 catgcggcgc atctagcgtc atgggccgat cgatggcaga ccattccgag atgaacaagc
      181 ccgccaggtc gcgtgcccgg ccgctgtccg aactcctcgg caagacgctg ggtgatgcct
      241 tcgcccggca gggcttcgca tcgaccggat ttgtgacgcg ctgggccgac atcgtcgggc
      301 ccgagatcgc cgcccactcc cagccggaaa aaatccagtg gccgccgccg cgcgatggcc
      361 agaccgccga acccggtacc ctggtgctgc gggtcgaagg gccgaccgcg ctggagattc
      421 agcacctgtc cagggtcatt ctcgaacggg tcaaccgctt ttttggctgg caggccattg
      481 ccgccatcag gttgcggcag gctccgctac ggcgcgatcg gcgccggccg ccgcctggac
      541 ccgatcccgc cgccaccgag gcccttgccc ggtctctccc cgaaattgag gatgaaaagc
      601 tgcggcaggc gcttgccagg ctgggcgccg ccatcaaaca gccgtgagac cgcgcgaggg
      661 tgttccgccc ggcgcgccat tgccacaatt gccttttcag gatagcgaca ttccggctcc
      721 ggccgcctgt agctgaagga atacgatcgt ggatatctcg cgtcgaaacc tgctgatcgg
      781 gaccgcaacg gtcgtgatcg ccgcggccgc tggcggcggc tattacgcct ggagccagag
      841 caggtcgccg gcgacgccgg cccctaccgc gccaggggca accctgccca gcggagccgc
      901 cgccgaactg atggagccgg ggccgcttgg cgagcaggcg gtcggcaatg cgaatgctcc
      961 ggtcaccatc atcgagtacg cctcgatgac ctgcccgcac tgcgcccagt tccacgagca
     1021 agtctatcct gagctaaaga agaaatacgt cgacaccggc aaggtccgct tcatcttccg
     1081 cgaattcccg ctcgacccgc tggccgccgc cggcttcatg ctggcgcgtt gcggcgacaa
     1141 ggacaaatat ttcccgatga tcgagacgtt gttcgcccag cagaaggact gggtggtgca
     1201 gaagccgctg gagccgctgc tggcaatcgc caagcaggcc ggcttcaccc aggagtcgtt
     1261 cgacaagtgc ttggcgaatc aaaaagttct ggacggcatc gaggaggtcc gcacccgggc
     1321 ggcgcagaag tttcaagtca cctcaacgcc gacgtttttc gtcaacggca agatgctccg
     1381 cggctcgccg acgctggagg agctgagcaa gcagatcgag gccaatctca agagctgatt
     1441 agggtttttg gccgatactg ggattcggat atccggctgc aagcggccgg ctgcgatttg
     1501 ggttgaataa cgcgttcttt ttgtttgcga aatcacgggc ggcgattcat tctcccggcc
     1561 gatgcggcag aaccttcacc actcgaccca ccccgtgggc ccgagacctg aggatgattc
     1621 ccttggggct gggctcattt tgaatggagc agcgtcggca tgaagctcac gcggctgcgc
     1681 ctgcttggct ttaagtcatt cgtcgaaccg accgatttcc tgatcgagcc gggcctcacc
     1741 ggcgtcgtcg ggcccaacgg ctgcggcaag tcgaacctgg tcgaagcgtt gcgctgggtg
     1801 atgggcgaga cctcgcacaa gtcgctgcgc gccgccgaca tggaagatgt gatcttcgcc
     1861 ggcaccaaca accggcccgg gcgcaacaac gccgaagtcg cgatgcacat cgacaacagc
     1921 gaccgcaagg cgccggcgca gttcaacgat accgaaagcc tcgacgtctc gcgccggatc
     1981 gagcgcgaga agggctcgag ctatcgcatc aacggccgcg aggtccgcgc ccgcgacgtg
     2041 cagatcctgt tcgccgacgc ttccaccggc tcgcgttcgc ctgcgctggt gcatcagggc
     2101 cggatcggcg agatcatcca ggccaaaccc gagcagcgcc gccgcgtact ggaagaggcc
     2161 gccggcattt caggcctgca cgcccgccgc cacgaggccg agttgcggct gcgcgccgcc
     2221 gagcagaacc tgctgcggct ggaagacgtc atcaaccagc tcaacgccca gatggcgtcg
     2281 ctgaagacgc aagcacgcca ggcggtgcgc taccgcaaca tcgccggcca gatccgccgc
     2341 accgaggcaa ccctgttcca cctgcgctgg accgcggcga gcgccgagct tgccgcagcc
     2401 gagcaggcca tgaacgaagc ggtgcgagtc gtcgccgatc gtaccggcgc gcaggccgaa
     2461 gccgcgaccc gtcaggctct ggcggcggcc gaactgccag cgctgcgcga agccgaagcg
     2521 gcggcggccg ccgggctgca tcgccttgtg gttgcgcgtg agaccttgga gaaggaagag
     2581 gcgcgggcgc gcgatcgcat cgccgaactg gaccgccggc tggtgcaact cgagggcgac
     2641 atcgagcgcg agcgccagca cgtagtcgat gccgaagcgg cgctgaccag acttgccgcc
     2701 gaagaggaat cgctcaagca ggacatcgac gccaatgcgg aaaagcggac cggcgccgac
     2761 gcgcgcgtcg ccgaggcggg cgcggtgctg ggcgtcgcgg aaaagacctt cggcgaactc
     2821 accgggacgc tcgccgacct gactgcgcgt cgcaaagcgc tggagcaggc cgcgcgcgcc
     2881 catgacgacc ggctcgcccg gctggcgaat gaaaccgccg cggtcgaggc cgaactggct
     2941 tcgctcgagg ccgccaatgc gggccatccc gacgtcgcgg cactcgcgat cgccgccgaa
     3001 gaggcgcagg ccgcggtgac ggaagccgag gccgcatccc tgcgcgccga agccgcgcat
     3061 tcggcggccc ggcaggcgct cgacgtcgcc cgcgggccgc tggccgaagc cgagcggcgc
     3121 gccggccgtc tcgataccga ggccaagacg ctggccaagc tcctgcacgt cgacaccaag
     3181 aacctgtggc cggccgtgat cgacgacctc aaggtcgaga agggctacga ggccgcgctc
     3241 ggcgccgcgc tgggcgacga cctcgaggca ccggtcgatc cgtcggcacc gatgcgctgg
     3301 gccggcgccg agattgatcc gtccgacccc gtactaccgg acggcgccga gccgcttggt
     3361 gcctatgtct cggcacccaa agagatggcg cgccggctgg cgcagatcgg cgtcatcgtg
     3421 cgcgaggacg cgcttcgtct gatgcccgcg ctgaagcccg gccagcggct cgtttcccgc
     3481 gacggcgatc tgtggcgctg ggacggtttc tcggtcgccg ccaatgcgcc gaccggcgca
     3541 gcgcggcggc tcgccggcaa gaaccggctt gccgacatcg agaccgaact ttcgaccgtg
     3601 cgccacgacg tcgaagcccg ccagcaggcg gcggcgcacg cgcaggccga agtcactgcc
     3661 gccgccgccg ccgaaaccgc tgcgcgcaac cgctggcgcg aactgcagca ggccgcgcat
     3721 gcggcgcgca accgtcacgc cgaggtcgag cgcgaagtca gccgcaattc ggcacgccgc
     3781 tcggcgctcg gcgaagccaa ggcgcggctc accgcgagcc gcgacgaggc cgcgatcgcg
     3841 cggggcgacg tcgagcagtc gctcacggcc ttgccgccgt cggcggacat cgaggagcga
     3901 ctcgccgccg tcaagacgga gatcgaaggc caccggacgc gggtcgcgga ggtgcgggcg
     3961 gaagcgcagg ggctcgcgcg cgaagccgag atggcggccc ggcgcctcgt caccatcggc
     4021 tcggagcgcg agagctggac ggcccgcaac gccggcgcca cctcgcagct tgagaccctc
     4081 tctgtccggg tcgaggaagc gaaggccgaa cgcgcggacc tcgaaaatgc gccgcaggtg
     4141 tttgccgagc agcgcacggc gctgatcggc gagatcgaga cggcggaagg caaccgccgc
     4201 gccgccgccg accggctggc ggaagccgag acccggctgg ccgaggccga ccgcgacgcc
     4261 cgtgcggcgc tcgacgcaat gggcagcgcg cgcgaagacg cggcgcgagc cgaggagagg
     4321 ttcgaggccg gcaagcggcg gcttgccgac atcgatcacg agatcgtcga gatgttggag
     4381 gtggcgccgg ccgaggtcgc gggtctcgcc gaactcaatc ccgacaagcc gctgcccgag
     4441 gtggcggagg tcgagtcgac cttggaacgg ctgcggcgcg aacgcgagcg gctcggcgcc
     4501 gtcaacctgc gcgccgacga ggaactacgc gaggtcgaga cccagcacac cacgctgacc
     4561 accgagcgcg acgacctggt cgaggcgatc aagcggctgc gccagggcat ccagagcctg
     4621 aaccgcgaag cccgcgagcg gctgctcgcg tcgttcgagg tggtgaacaa ccacttccgc
     4681 gagctgttca cccacctgtt cggcggcggc accgccgaac tcaagctgat cgaggccgag
     4741 gatccgctgg aagccgggct cgagatcatc gccaagccgc ccggcaagaa gcccgcgacg
     4801 ctgtcgctgc tctcgggcgg cgaacaggcg ctgaccgcgc tggcgctgat cttcgcggtg
     4861 ttccgcacca acccggcacc gatctgcgtg ctggacgagg tcgacgcccc gctcgacgat
     4921 cacaacatcg agcggttctg cgacctactc gacgagatga cgcgcgcgac cgacacccgc
     4981 ttcgtgatca tcacgcacaa tccgatcacc atggcgcgga tgaatcggct gttcggcgtc
     5041 accatggcgg agcgcggcat ctcgcagctg gtctcggtcg atctcgaagg cgccgtgcgg
     5101 ctgcgcgaag cgagctgacc taacgcctgc gatcaaatca ggtagagcgg catcaaccct
     5161 cccccttgtg gggagggtcg cctgccgcgc gatgcgcggc aggcggggtg gggtcttgta
     5221 gggggagggg agcagaccgc cgccgttgct gccttcttcg acttaaactc atcgtgcatc
     5281 agtccgcggc ggcgagcggc cggctgacgc tctccagcgg cttgcgctcg gcggcg
//
LOCUS       DASTZO010000222         6019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_197723,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000222 DASTZO010000000
VERSION     DASTZO010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6019
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_197723"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1393
                     /locus_tag="VFU20_06240"
     CDS             <1..1393
                     /locus_tag="VFU20_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286093.1"
                     /translation="GEVLDFNLYSTDPGGTLGQEPTASSTSMFIKLDGVGTSEDMIII
                     LKLYDTVTGIYTTKAVVVQNADIIKSDAALTGTAYDSITLDNNDGLIVIEPNDYQEGN
                     TNLVIVGAQIAGSDEGITGSGINLNGAIGVGSTGTQPFDTDVSDAPFKITSIGFVTTS
                     TADQNASLSFDVTVEDADGDAVTQSIIVNIGDTTTTTSTASTAVVSQEPIEDTSAFSL
                     TSSDSLDQQRSMSAANNNSVLLGAIAAAGLGVGSAAAANGFAPGGDYATFGTTAFGYD
                     GGAMQGYTLDSRDIVSDAFGGESLEIAAGDYSGGPSASHSAIVEKLSLTDAGGEQSLA
                     PIELPQGTEIQQPDPAQSAFTSAVVGMPSAEMLASANGNALDAQAQGTAEVGRVLIDA
                     LAGGGGPDIDAVLDAAANQVPGGNAALDALASHGAAAVSGWDTAGFAGFPGGHSSVTV
                     DPMLHHDAVVAAA"
     gene            1466..2887
                     /locus_tag="VFU20_06245"
     CDS             1466..2887
                     /locus_tag="VFU20_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5286094.1"
                     /translation="MKRKHWTMIVPGAVLLCTSPAYAVELRDAVQAALSTNPEIRQAV
                     ANREATREERVQAQGLWHPRVSVEGSAGVRNLRNPTRRNIGIADETLWPIEGELIVDQ
                     LLFDSGGREAEIRRQAARTDAAAARVEERSEFVALNVSRTYIDYLLQQRLVAIAEDNA
                     TFHERLSGDLREGVSKGSISIADQQQAEERLQSARARVTEAREDLENAGITFRTLTGL
                     PVDSVSMPPDLSQCQPATLEEAEALARQNNPRVQEAIADLATAREDIRAARADIGPRV
                     NLEGRARAGHDIDGFAGRTTDLLANVVLRWDLYNGGIKEANVREQRNRADEVHGRLFE
                     RTRRAEEDVRTAWSRLQNQSRLVSELETQQRIADDLLLSYREQFNIGRRSLLDVLDAQ
                     NTRNNVQAQTETARLAKLYAQYRVLASTNRLIECLGVSMPTAAYSEERTRFRVNPIPP
                     ADLDENSYPLPRMGPPAPAGGSQ"
     gene            2912..5059
                     /locus_tag="VFU20_06250"
     CDS             2912..5059
                     /locus_tag="VFU20_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020821157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HEU5286095.1"
                     /translation="MHWLDAQPSREIDPVLDCLTYLAREADRPSSPVLLRAGLALSEK
                     GTLPFHQVEPALEQVGMRGDPVSRKLKGWPSRKLPAILELEDDRAAVLLDMRDGDGLI
                     YAPGVAEPLWAPLSEIEAVFTGRAVVVEADPARERENERPWDKARRTHWFWSEVWKVR
                     REFWPVILAALIVNMLALAMPLFTMNVYDRVIPNKAVSTLWVLAAGVGLALLFDFILR
                     LARAQLIDEIGRKLDAKLAQKLFEKVMNLPMAARQGSTGALARRVSEYELVRDFFAST
                     TVVLAVDVLFLFLFLILITVLAGWLVVVPIVGIVLMFAAGMSLQRSMGRAAIDAQADS
                     SLQNSLLVESIAGAETLKAARAEGQMLGRWRRYSGMSATTQERMRRLTAVAVNLAGVS
                     QQLISVGLLIGGFYQFNQGDMTMGAIIAIIMLAGRAIQPIGQLAFLFTRGRQAFATLD
                     SLQEMMDVADERQSSMRSIMPEIRAGHIKLDNVDFRYPNAAKDALSGLNLTVRPGERI
                     GIIGRVASGKSTLGRVLCGLYEPTGGTYTVDGLDSRQFHPHQLREAFRFVGQDSELFS
                     GSVRDNLMLGAAQATDNQLVDAVVRSGADIFLSRDAAGFDLAVGERGSQLSGGQRSLL
                     VLARALVSPCKLLFLDEPSGAMDTQTELYFIEHLKTALAPDQTLMVATHRHNMLSILD
                     RLIVIDAGRIIADGPRDQVLGHLGGTPKKDSKK"
     gene            5056..>6019
                     /locus_tag="VFU20_06255"
     CDS             5056..>6019
                     /locus_tag="VFU20_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HEU5286096.1"
                     /translation="MTSLAIARRRLAARLTEYKVEAIAFAVVMLAGLAIGAVGFMAPA
                     GAADDSVSIQLTPAKAAAMVAATSASPAEIRAIGEEARLINAALPFSTTPVQAARPFE
                     IGGDDLDQRRALLCLTQAVYYEAGFEPLAGRRAVAQVVLNRMRHPAFPKSVCGVVYQG
                     SRSPVCQFSFVCDGSLYRAPATGAWRDAKAVAEAALGGYVEKSVGASTHYHANYVAPR
                     WAPMLTKITQLGAHIFYRWPGAWGQSAAFSGRYVGEPQDPLALRPPLRAPLQPGTPEQ
                     LAAIAEAEAGPPVARAPNDVGGLLDVSKGWTLNIPGPDEIGAATA"
BASE COUNT          983 a         2082 c         2024 g          930 t
ORIGIN      
        1 cggggaggtg cttgatttca acctctactc gaccgatccg ggcggtacgc tgggccagga
       61 accaactgcc agctccacgt cgatgttcat caagctggac ggcgtcggta cttcggaaga
      121 catgatcatc atcctgaagc tctacgacac ggtcactggt atctacacga ccaaggccgt
      181 ggtggttcaa aatgccgaca tcatcaaaag tgacgcggca cttacgggga ccgcgtacga
      241 cagcatcacg ctcgacaaca acgacggact catcgtgatc gagcccaacg actatcagga
      301 aggcaatacc aacctggtga tcgtcggcgc gcagatcgcg ggttccgatg aaggcatcac
      361 cggctccggc atcaacctca atggagcgat cggcgtcgga agtaccggca cgcagccatt
      421 tgatacggac gtgagtgatg cgccgttcaa gatcacctcg atcggctttg tcaccacgtc
      481 gaccgcggat cagaacgcaa gcctgtcgtt cgatgtcacg gtcgaggacg cggacggcga
      541 cgccgttacc cagtcgatca tcgtcaacat cggcgacacg acgaccacga ctagcactgc
      601 ttcgacggca gtcgtcagcc aggagccgat cgaggatacg tcggccttct cgctaacctc
      661 ctcggacagc ctcgaccagc agcgttcgat gagcgccgcc aacaacaaca gcgtgttgtt
      721 gggcgcgatc gccgcggcgg ggctgggcgt gggttcggcg gcggccgcca acggcttcgc
      781 tccgggcggc gactatgcga cgttcggcac gacggcgttc ggctacgacg gcggcgccat
      841 gcaaggctac acgctcgaca gccgcgacat cgtcagcgat gcctttggcg gcgaatcgct
      901 ggagattgcc gccggcgact attccggcgg tccttcggcc agtcattcgg cgatcgtcga
      961 gaagctgtcg ctgaccgatg ccggcggcga gcagtcgttg gcgcccatcg agctgcccca
     1021 gggcaccgag atccagcagc cggacccggc gcagtcggcc ttcaccagcg ctgtggtcgg
     1081 gatgccgtcc gcggaaatgc tcgcgagcgc gaacgggaat gcgctcgacg cgcaggccca
     1141 gggcacggcg gaggtcggcc gggtgctgat cgacgcgctt gccggcggcg gcgggccgga
     1201 tatcgacgcg gtgctcgatg ccgcggcgaa tcaggtcccg ggcggaaacg cggcgctgga
     1261 cgcgcttgca agccacggcg ctgccgccgt ttcaggctgg gacacggccg gttttgccgg
     1321 cttcccgggc ggccattcat cggtcacggt ggatcccatg ctccatcatg atgcggtggt
     1381 tgccgcggcc tgatcgagat ttgaagccag agggtctccg ctggtggacg gtggagaccc
     1441 aaactgaaga ccagaagggg gaattatgaa aaggaagcac tggacgatga tcgtccccgg
     1501 ggcggtgctg ctgtgcacgt caccggccta cgcggtcgag ctgcgcgacg cggttcaggc
     1561 tgccctcagc accaacccgg agatccgcca ggcggtcgcc aaccgggaag ccacccgcga
     1621 agagcgggtc caggcccagg ggctgtggca tccgcgcgtt tccgttgagg gatccgcggg
     1681 cgtccgcaac ctgcgcaacc cgacccggcg caacatcggc atcgccgacg agaccctgtg
     1741 gccgatcgag ggcgagctga tcgtcgacca attgctgttc gacagcggcg gccgcgaggc
     1801 ggaaatccgc cgccaggccg cgcgcaccga cgccgccgcg gcccgcgtcg aggagcgctc
     1861 cgagttcgtc gccctcaatg tctcgcgcac ctatatcgac tatctcctcc agcagcggct
     1921 ggtggcgatc gcggaagaca atgcgacctt ccacgagcgg ctttcaggcg accttcgcga
     1981 aggcgtttcg aagggctcga tcagcattgc cgaccagcag caggccgaag agcggctgca
     2041 atcggcccgc gcccgggtca ccgaggcccg cgaggaccta gagaacgccg gaatcacctt
     2101 ccgcacgctg accggccttc cggtcgacag cgtgtcgatg ccgcccgacc tgtcgcagtg
     2161 ccagcccgcg acgctcgagg aggccgaagc tctggctcgg cagaacaacc cgcgcgtcca
     2221 ggaagccatc gccgacctcg ccacagcgcg cgaggacatc cgggccgccc gcgccgacat
     2281 cggtccgcgg gtcaacctcg agggccgggc ccgcgccggg cacgacatcg acggctttgc
     2341 cggccgcacc accgacctgc tcgccaacgt cgtccttcgc tgggacctgt acaatggcgg
     2401 catcaaggag gcgaacgtcc gcgagcaacg caaccgggcc gatgaagtgc acggccggct
     2461 gttcgaacgg acccgtcgcg ccgaggaaga cgtgcggacc gcctggagcc ggctccagaa
     2521 ccagagccgg ttggtgagcg agctcgagac ccagcagcgt atcgccgacg atttgctgct
     2581 gtcctaccgc gagcagttca acatcggccg ccgctcgctg ctcgacgtgc tcgatgcgca
     2641 gaacacgcgc aacaacgtcc aggcccagac cgagacggcc cggctggcca agctgtatgc
     2701 ccagtatcgc gtgctggcgt cgaccaaccg gctgatcgaa tgccttggcg tttcgatgcc
     2761 gaccgctgcc tacagcgagg aacggacgcg cttccgcgtc aaccccatcc cgcccgcgga
     2821 tctcgacgag aacagctatc cgctgcctcg catgggaccc ccggcgccgg ctggcggcag
     2881 tcagtaactc tgggggcggg tagtgagttt catgcattgg ctcgacgcac agccgtcgcg
     2941 tgaaattgac cccgtcctcg actgcctgac gtacctcgcg cgcgaggcgg accggccgtc
     3001 ctcgccggtg ctcttgcgcg ccggtctcgc actttccgaa aagggcacgc tgccgttcca
     3061 ccaggtcgag ccggcgctcg agcaggtcgg gatgcgcggc gacccggtca gccgcaagct
     3121 caagggctgg ccgtcgcgca agctgccggc gatcctcgag ctcgaggacg accgggccgc
     3181 ggtcctgctc gacatgcgcg acggcgacgg gctgatctac gcgcccggag tcgccgagcc
     3241 cttgtgggcc ccgctgtccg agatcgaggc ggtgttcacc ggccgagcgg tcgtcgtcga
     3301 agccgacccg gccagggagc gcgagaacga gcgcccgtgg gacaaggcgc ggcggaccca
     3361 ctggttctgg tccgaagtgt ggaaagtccg gcgcgagttc tggccggtca tcctcgccgc
     3421 gctgatcgtc aacatgctgg cgctggcgat gcccctgttc acgatgaacg tctacgaccg
     3481 ggtgatccct aacaaggcgg tgtcgaccct gtgggtgctc gccgccggtg tcggcctggc
     3541 gctgctgttc gacttcatcc tgcgcctcgc gcgggcgcag ctgatcgacg agatcggccg
     3601 caagctcgac gcgaagctcg cccagaagct gttcgagaag gtgatgaacc tgccgatggc
     3661 ggcgcgccag ggcagcaccg gggcgctcgc ccggcgggtg tcggaatatg agctggtccg
     3721 cgatttcttc gcctcgacca cggtcgtcct ggcggtcgac gtcctgttcc tcttcctgtt
     3781 cctgatcctc atcacggtgc tcgccggctg gctggtggtg gtgccgatcg tcggcatcgt
     3841 gctgatgttc gcggccggga tgagcctcca gcggagcatg ggccgggcgg cgatcgacgc
     3901 gcaggcggac tcgagcctcc agaactcgct gctggttgaa tcgatcgccg gcgccgagac
     3961 cctcaaggcg gcgcgcgcgg aaggccagat gctcggccgc tggcggcgtt attcgggcat
     4021 gtcggcgacg acccaagagc ggatgcggcg gctgaccgcg gtcgccgtca acctcgccgg
     4081 agtctcgcag cagctgatca gcgtcgggct tctgatcggc ggtttctacc agttcaacca
     4141 gggcgacatg acgatgggcg cgatcatcgc catcatcatg ctcgccggcc gcgccatcca
     4201 gccgatcggc cagctggcgt tcctcttcac gcgcgggcgg caggcgttcg cgaccctcga
     4261 ttcgctccag gagatgatgg acgtcgccga cgagcggcaa tcgtcgatgc gcagcatcat
     4321 gcccgagatt cgcgccggcc acatcaagct ggacaatgtc gatttccgct atccgaacgc
     4381 ggccaaggac gcgctgtcgg gcctcaacct cacggtccgt cccggcgagc ggatcgggat
     4441 catcggccgg gtcgcgtcgg gcaagtcgac cctcgggcgg gtgctgtgcg gcctgtatga
     4501 gcccaccggc ggcacctaca ccgtcgacgg gctcgacagc cgccagttcc acccgcacca
     4561 gctgcgcgag gcgttccgct tcgtcggcca ggattccgag ctgttcagcg gctcggtccg
     4621 cgacaacctg atgctcggcg ccgcccaggc gaccgacaac cagctggtcg acgccgtcgt
     4681 ccgttcgggc gccgacatct tcctgtcgcg cgatgcggcc ggcttcgacc tcgcggtcgg
     4741 cgagcgcggc tcgcagctgt cgggcggcca gcgctcgctg ctggtgctgg cccgggcctt
     4801 ggtctcgccg tgcaagctgt tgttcctcga cgagccgagc ggggcgatgg acacccagac
     4861 cgagctctat ttcatcgagc atctcaagac ggcgctggcg cccgaccaga cgctgatggt
     4921 cgcgacccac cgccacaaca tgctgtccat cctcgaccgg ctgatcgtca tcgacgccgg
     4981 gcggatcatc gccgacgggc cgcgcgacca ggtgctcggc catctcggcg gaacgccgaa
     5041 gaaggattcg aagaaatgac gtcgctcgca atcgcccgcc gccggcttgc ggcccggctg
     5101 accgaataca aggtcgaggc gatcgccttc gcggtggtga tgctcgccgg cctggccatc
     5161 ggcgccgtcg gcttcatggc gccggccggg gcggcggacg acagcgtctc gatccagctg
     5221 acgcccgcca aggccgcggc gatggtcgcc gcgaccagcg cttcgcccgc ggaaatccgc
     5281 gcgatcggcg aggaagcgag gctgatcaac gcggcgcttc cgttcagcac gacaccggtc
     5341 caggccgccc gcccgttcga gatcggcggc gacgacctcg accagcgccg ggcgcttctg
     5401 tgcctgaccc aggcggtcta ttacgaggcc gggttcgagc ccctggccgg ccgccgggcg
     5461 gtcgcccagg tcgtgctcaa ccggatgcgc cacccagcct tcccgaaatc ggtctgcgga
     5521 gtcgtctacc agggctcgcg ctcgccggtc tgccagttca gcttcgtgtg cgacggctcg
     5581 ctctaccgag cgccggccac gggcgcgtgg cgcgacgcca aagcggtcgc cgaggcggcg
     5641 ctgggcggtt acgtcgagaa atcggtcggg gcgtcgaccc attatcacgc caattacgtg
     5701 gccccgcggt gggcgccgat gctgaccaag atcacccagc tgggggcgca catcttctac
     5761 cgctggccgg gcgcctgggg tcagagcgcc gctttctccg gccgctatgt cggcgagccg
     5821 caggatccgc tggcgcttcg cccgccgctg cgcgcgccgc tccagccggg aaccccggaa
     5881 cagctggccg cgatcgccga agcggaggcc gggccgccgg tcgcccgcgc tcccaacgac
     5941 gtcggcggac tgctcgacgt ctccaagggc tggaccctca acatcccggg gcccgacgag
     6001 atcggcgccg ccaccgccc
//
LOCUS       DASTZO010000223         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1037638,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000223 DASTZO010000000
VERSION     DASTZO010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1037638"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1548)
                     /gene="yidC"
                     /locus_tag="VFU20_06260"
     CDS             complement(<1..1548)
                     /gene="yidC"
                     /locus_tag="VFU20_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU5286097.1"
                     /translation="MSDDNKNMILAIVLSALVLLGWSLVSDKLLPTAGPQTVRVENGK
                     AKPLPQPQADPAADRPQALRNRAIVLASTPRVRIETPALKGSINLKGARIDDLVLLRE
                     REGIAKDSPPVRLLSPAGAPAGYFASFGWTGEGTQAPGPDAVWTTSAPILSPGRPVTL
                     SWASPSGLKFEQTIAVDDDYLFTVRQRVANPSPNAVAVRPYGLISRANESPDPDGWTM
                     HVGPIGFLDGAANYDVDWDTLDEAGAEGERFASRGGWLGFTDKYWLAALAPAQDAAIS
                     ASFRRAPNGPYQADFARQPIIVPPGQAIAIESHLFAGAKEKGLLDRYEAMGIPKLSKS
                     IDWGWFEWFMRPIFDLLNWLFKQIGNFGVAIICLTFIVRLLMYPIAQKQFQSMAAMRK
                     VQPKMKAIQERYKDDKPRQQQEILKLYQTEKINPAAGCLPILLQIPVFYALYKVLLVS
                     VEMRHQPFALWIKDLSAPDPATVLNLFGLLPFDPPGFLAIGILPILLGVTMWLQFKLN
                     PQQMDPTQ"
     gene            complement(1594..1806)
                     /gene="yidD"
                     /locus_tag="VFU20_06265"
     CDS             complement(1594..1806)
                     /gene="yidD"
                     /locus_tag="VFU20_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU5286098.1"
                     /translation="MIARLLIWVARSWQAGPSKVLPPSCRYTPSCSAYAITALSRYGA
                     ARGGWLALKRICRCHPWGGQGYDPVP"
     gene            complement(1803..2114)
                     /gene="rnpA"
                     /locus_tag="VFU20_06270"
                     /pseudo
     CDS             complement(1803..2114)
                     /gene="rnpA"
                     /locus_tag="VFU20_06270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
     gene            complement(2126..2260)
                     /gene="rpmH"
                     /locus_tag="VFU20_06275"
     CDS             complement(2126..2260)
                     /gene="rpmH"
                     /locus_tag="VFU20_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005980833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU5286099.1"
                     /translation="MKRTFQPSNLVRKRRHGFRHRMATPGGRKLLNARRARGRKKLSA
                     "
     gene            complement(2348..2785)
                     /locus_tag="VFU20_06280"
     CDS             complement(2348..2785)
                     /locus_tag="VFU20_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286100.1"
                     /translation="MMTREQWREFVRSRPVRTALFMTGLLLLILSPLAGAIPGPGGIF
                     VFAAGLTLTLKNSDWAKRQYVRFMAWQPKAGRWADWGLRRRSARRREERRKLRRALGH
                     PPTPDELRQGPLAALIPDPPAKAPEKWGDREGRADRPSRDPAN"
     gene            2926..>3635
                     /locus_tag="VFU20_06285"
     CDS             2926..>3635
                     /locus_tag="VFU20_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010235940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HEU5286101.1"
                     /translation="MGATVATVAYLGLEARAVEVQVQLSSGLPAFIIVGLPDKAVAES
                     RERVRAALAAIGLALPPKRITVNLSPADLPKEGSHFDLPIALCLLAAIGAADAESLSH
                     YVAVGELSLDGRIAVSPGVLLAALHASAHDKGLICPAAQGSEAAWAGELDVLAAPDLL
                     ALLAHLKGTALLPAPAVAEVEPLGHGPDLSQVKGQEVAKRALEIAAAGGHNLLMSGPP
                     GAGKSLLAACLPRILPPL"
BASE COUNT          528 a         1265 c         1277 g          565 t
ORIGIN      
        1 ctgagtcggg tccatctgct gcgggttcag cttgaactgc agccacatcg tcacccccag
       61 caggatcggg aggatgccga tggcgaggaa ccccggcggg tcgaacggca gcagcccgaa
      121 caggttcagc accgtcgccg gatcgggcgc ggacaggtcc ttgatccaca gagcgaacgg
      181 ctggtggcgc atttcgacgc tcaccagcag caccttgtag agcgcgtaga agaccgggat
      241 ctggagcagg atcggcaggc agccggcggc cgggttgatc ttctcggtct ggtagagctt
      301 caggatctcc tgctgctggc gcggcttgtc gtccttgtag cgctcctgga tcgctttcat
      361 cttcggctgc accttgcgca tcgccgccat cgactggaac tgcttctggg cgatcgggta
      421 catcaagagc cggacgatga acgtcaggca gatgatcgcc acgccgaaat tgccgatctg
      481 cttgaacagc cagttcagga ggtcgaagat cggccgcatg aaccattcga accagcccca
      541 gtcgatcgac ttcgacagct tggggatgcc catcgcctcg tagcggtcga gcagcccctt
      601 ttccttggcg ccggcgaaca gatggctctc gatggcgatc gcctggccgg gcgggacgat
      661 gatcggctga cgcgcgaaat cggcctggta ggggccgttc ggcgcccttc ggaagctcgc
      721 cgagattgcc gcgtcctggg ccggggccag cgccgccagc caatatttgt cggtgaatcc
      781 gagccagccg ccgcggctgg cgaagcgctc gccctcggcc ccggcctcgt cgagcgtgtc
      841 ccagtcgacg tcgtaattgg cggcgccgtc cagaaagccg atcggaccga cgtgcatcgt
      901 ccagccgtcg gggtcgggcg attcgttggc ccggctgatc agcccgtagg ggcgcaccgc
      961 gaccgcattg gggctcgggt tggcgacccg ctggcggacc gtgaacagat aatcgtcgtc
     1021 gaccgcgatg gtctgctcga atttcaggcc gctcgggctg gcccagctca gggtcaccgg
     1081 gcgccccggt gaaaggatgg gcgcgctcgt cgtccacacg gcgtcggggc cgggcgcctg
     1141 cgttccctcg ccggtccagc cgaagctcgc gaaatagccg gcgggggcgc ccgccggcga
     1201 cagcagcctc accggcggcg aatccttggc gatcccctcg cgctcgcgaa gcagcacaag
     1261 gtcgtcgatc cgggcgccct tcaggttgat cgaccccttt aaggcggggg tctcgatccg
     1321 cacccgcggg gtcgaggcga gcacgatcgc ccggttcctc agcgcctggg gccggtcggc
     1381 ggccgggtcg gcctgcggct gcggcagcgg tttcgccttg ccgttctcga cccgcaccgt
     1441 ctgcggcccc gcggtcggca gcaatttgtc cgacaccagg ctccagccga gcagcaccag
     1501 ggcgctcagc acgatggcga ggatcatgtt cttattgtcg tcgctcaact cggaatcctc
     1561 gtcatgggac cggccttttc atgggaccgg ccttcatggg accgggtcat agccctggcc
     1621 gccccacggg tggcaccggc agatgcgctt caacgccagc cagccgcccc gcgcggcgcc
     1681 atagcgcgac agagcggtga tcgcataggc cgagcagctc ggagtgtagc ggcagctcgg
     1741 cggaagcact ttcgacggcc ccgcctgcca gctgcgggcg acccagatca gcagccgggc
     1801 gatcatttcc gaaggcgctc cagggcgccc gtcagctcca gccgaagctg cgcgaagtcg
     1861 cgctcgatgc cgcccgagcg gccgatcagc acatggtcgg cgcccggaaa gccgccctcg
     1921 ggcaggatct cgcgggccag cgcccgaagc cggcgcttca tccggttgcg gacgacggcg
     1981 ccgccgatct tcctggtgac ggtgaagccg acgcgcttgg cggggtcgcc gtcggcgcgg
     2041 tcgcggacct gcagcacgaa gccgggggtc gccgcgcgct tgccggcatt ggcagcgagg
     2101 aaatcggagc gcttgcggag cgtggctagg cgctgagctt cttgcggccg cgcgcccggc
     2161 gcgcgttcag cagcttgcgc ccgcccggcg tcgccatgcg gtggcggaac ccgtggcgcc
     2221 gcttgcggac cagattgctc ggctggaagg tgcgcttcat cgctcatgcc ttaatggatc
     2281 aaagaaaaag ggccgccgtt cgggcagccg caatcgggtg cgccgatagt ggaggggcgc
     2341 gtcaaagtca attggccggg tccctcgaag gccggtcggc gcgcccctcg cgatcgcccc
     2401 atttctcggg cgccttggcc ggcggatcgg ggatcagcgc cgcgagaggc ccttgccgaa
     2461 gttcgtcggg cgtcggcggg tggcccagcg cccggcgcag cttgcgccgc tcctcgcggc
     2521 gccgcgcgct gcgccgcctg aggccccagt cggcccagcg cccggccttc ggctgccagg
     2581 ccatgaaacg gacgtactgg cgcttcgccc agtcgctgtt cttgagcgtc agggtcaggc
     2641 ccgcggcaaa gacgaaaatg cccccggggc cggggatcgc gccggcaagc ggcgagagga
     2701 tgagcagcag caggccggtc atgaacagag cggttcgaac cggcctgctg cggacgaact
     2761 cccgccactg ttcgcgcgtc atcatcggat ggacatgtgg ttgcatcggc ttgccggcgc
     2821 aagggacgct tgccgatagc ggcccgcagg gtctagcgtg cccgggccga gctaggagtc
     2881 cctctgaaca cgcaatcccg gtcagctgcc gtggcgcggc gctgaatggg cgcgaccgtc
     2941 gccaccgtgg cgtacctcgg cctcgaggca cgggccgtcg aagtgcaggt ccagctctcc
     3001 tcggggctgc cggccttcat catcgtcggc ctgcccgaca aagcggtcgc cgagagccgc
     3061 gagcgggtgc gcgcggcctt ggcggcgatc ggcctcgcgc ttccgcccaa gcggatcacc
     3121 gtcaacctgt cgccggccga cctgcccaag gaaggctcgc acttcgacct gccgatcgct
     3181 ctgtgcctgc tggccgcgat cggcgccgcc gacgccgaga gcctgtcgca ttatgtcgcg
     3241 gtcggcgaac tcagcctcga cggccggatc gccgtctcgc ccggggtgct gctcgcggcg
     3301 ctgcatgcgt cggcccacga caagggcctg atctgcccgg ccgcgcaggg cagcgaggcg
     3361 gcctgggccg gcgagctcga cgtgctcgcg gcgcccgact tgctggcgct gctcgcgcac
     3421 ctgaaaggca ccgccttgct tccggccccg gccgtcgccg aggtcgagcc gctcggccac
     3481 ggccccgacc tcagccaggt caagggccag gaggtcgcca agcgggcgct cgagatcgcc
     3541 gccgccggcg gccacaattt gctgatgagc gggccgcccg gggccggcaa gtcgctgctc
     3601 gctgcctgcc tgccgcggat cctgccgccg ctcga
//
LOCUS       DASTZO010000224         4016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_175896,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000224 DASTZO010000000
VERSION     DASTZO010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4016
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_175896"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="VFU20_06290"
     CDS             complement(<1..193)
                     /locus_tag="VFU20_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286102.1"
                     /translation="MASASMFAGELEKSKGIGVRRLTVATGATAATVFILCWLGTFVP
                     FSSPTHAYIGLFTIAGPSSV"
     gene            381..1376
                     /locus_tag="VFU20_06295"
     CDS             381..1376
                     /locus_tag="VFU20_06295"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU5286103.1"
                     /translation="MGNAIGLPARIVAAALAGALAGDGAHAQQVEIGTGSVVAAVSKL
                     RPGEFVWAPGIAPSGPLLLIVNTTTQRAALYRNGVPIAATTVSTGRPDYRTPTGVFTI
                     LQKHVEHYSNRYDNAPMPYMQRLTWSGIALHAGQLPGYPASHGCIRLPAAFARLLYGV
                     TRLGMTVVVTDQATTPRVAPTPEIALAGRELLPAPGEAVEWNPERSLSGPLSIVVSAA
                     DRRAVVLRNGVRIGSAPVSVEGPVTGTWAYALRATDSRGQHWLRVPLAGEGTAGSPVP
                     PEEWRRFHAPEEFRRAVAALVQPGTTIVVTADSLRAGDPDAVTVIEEEQPEPRAR"
     gene            1510..2757
                     /locus_tag="VFU20_06300"
     CDS             1510..2757
                     /locus_tag="VFU20_06300"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4010 domain-containing protein"
                     /protein_id="HEU5286104.1"
                     /translation="MDSTSPALLLGAALAAGLMIGIERGWRLRRYPAGTRVAGVRTFA
                     LVGAGGGIAALLGTMIHPGATLVLAVALLAGLGTGYWRDRAQHDATSFVAAVVALALG
                     LLAGAGQPALAVAGAAVVTLLLATREQSHGLVASLNRDDVHAFARYAVIAAAVLPFLP
                     NRNMGPLDSWNPFQLWLVVVLITGFSFAGYIASRTIGARKGVLATAIIGGAYSSTAVT
                     ASLARQLGRGAAGPFAAGILLASAMMYIRVVILMAILSPSTVAQFLVTVGPAAAVGMA
                     IALLAWWRAPQADEARAPVSGNPVELLPAFGFVAIVAAAAVATRWARERFGETGIAAS
                     LLFTGSFDVDAAIVTLSGLPVSAIDRRVAAIAIAGTIVANMAVKILVTAVYARRSGAA
                     AVAGMAASVAVLVLTIGWRLAIA"
     gene            complement(2754..3803)
                     /locus_tag="VFU20_06305"
     CDS             complement(2754..3803)
                     /locus_tag="VFU20_06305"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020820411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008720 - D-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0070404 - NADH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HEU5286105.1"
                     /translation="MKIALFSTKSYDRRFFDAANSGRHELRYLEPRLTSASAPLARGA
                     DAVCLFANDRSDRAAIETFAELGVRLIALRSAGFNNVDLSAAHSKGIAVCRVPAYSPH
                     SVAEHSFALILALVRKIHRAYNRVREGNFALEGLLGFDLAEKTIGIVGVGNIGAVVAT
                     IAKGFGCRILASDPVRRSECACLVDYVDLDTLLAESDIVTLHCPLNSATRHLIDSSAL
                     DRMKPGAILINTSRGAVVDTQAVIGALKRRRLGGLAIDVYEEEEALFFEDRSDQAIMD
                     DQFARLLTFPNVLVTGHQGFFTVEALANIARTTIANCDSFERSGVPLHPVPAPPKRRE
                     TEAAGPPAGALVTSS"
     gene            complement(3769..>4016)
                     /locus_tag="VFU20_06310"
     CDS             complement(3769..>4016)
                     /locus_tag="VFU20_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286106.1"
                     /translation="DLFYCRRRALEEQQAARLAACDAARHRHDELADMYRLRVAMLSK
                     PSEWRAGAWQWDELAEGPSGAAKVTGREDRPVQHQVV"
BASE COUNT          569 a         1388 c         1388 g          671 t
ORIGIN      
        1 ggacggagct cgggccggcg atggtgaaca ggccgatata ggcgtgggtc gggctggaga
       61 acggaacgaa ggttccgagc cagcagagaa tgaagacggt cgcggccgtc gccccggtgg
      121 cgacggtcag gcgccggacg ccgattccct tcgacttctc aagctctccc gcaaacatcg
      181 atgcactggc catgacaacc tccttcatcg cttcggatgg caggaggaca gtgccagctt
      241 ccagcggggc cgggcatccg taatggtccc tagggcgcaa tgccccggct cgcggctcgt
      301 ccgccgacca aagcgggtcc tcgggcgcta cggacctcta cctagcgact ccgcgggcgg
      361 ccgcgggcac catcgcggcc atgggaaatg cgatcggtct tccggcgcgg atcgtcgcgg
      421 ccgcccttgc cggcgcgctc gccggcgatg gcgcccacgc gcagcaggtc gagatcggca
      481 ccggctcggt ggtcgcggcc gtctcgaagt tgcgcccggg cgaatttgtc tgggcgcccg
      541 gaatcgctcc gagcggcccg ctgctgctga tcgtcaacac gaccacgcaa cgggcggcgc
      601 tgtaccgcaa cggagttccc atcgcggcga ccaccgtgtc gaccggccgg ccggattatc
      661 gcactccgac cggcgtgttc accatccttc agaagcacgt cgagcattat tcgaacagat
      721 atgacaatgc gccgatgccc tacatgcagc ggctgacctg gagcggaatt gcgctccacg
      781 cgggccagct tcccggctac cccgcttcgc acggctgcat ccgccttccg gcggcctttg
      841 cccgcttgct ctatggagtg acccggcttg gaatgaccgt ggtggtgacc gaccaggcga
      901 cgactccgcg agtcgctccg accccggaga tcgcgctggc gggcagggag ctcctgccgg
      961 cgcccggcga agcggtcgaa tggaatcccg aaagatccct gtccggcccg ctgtccatcg
     1021 tcgtcagcgc ggcggaccgg cgcgcggtcg tccttcgcaa cggggtcagg atcggctcgg
     1081 ccccggtcag cgtcgagggg ccggtcacgg ggacatgggc ctatgcactt cgggcgaccg
     1141 actcccgggg ccagcactgg ctgcgcgtgc cgctggcggg cgaggggacg gccggttcgc
     1201 cggtcccgcc cgaggagtgg cggcggttcc acgcgccgga ggagttcagg cgggccgtcg
     1261 ccgcgctcgt gcagcccgga acgacgatcg tcgtcaccgc cgattcgctt cgagccggcg
     1321 atccggacgc cgtcacggtc atcgaggagg agcagccgga gcctcgcgct aggtagcatt
     1381 acctattcac cgcgccgcgg acctccccga caatgagcgg tgacctgccg gcggactccc
     1441 cctccccagc ctgtccgccg ggaggcattt ccgtcatttt ttcggcccgc ttgatgtaag
     1501 tcctgctgcg tggactccac ttctcccgcc ttgctgctcg gggcagcgct cgcggccggc
     1561 ctgatgatcg gcatcgagcg cggctggcgg ctacggcgtt accccgcggg cacgagggtg
     1621 gcgggggttc gaaccttcgc gctcgtcggt gccggcgggg gaatcgcggc gcttctcgga
     1681 acgatgatcc atcccggcgc gaccctggtc ctggcggtcg cgctgctggc cggacttggc
     1741 accggctatt ggcgcgatcg cgcccagcac gacgcgacga gcttcgtcgc ggccgtcgtc
     1801 gcgcttgccc tcgggctgct cgccggcgcc gggcagccgg cgctggccgt tgccggagcc
     1861 gcggtcgtca ccctgctgct ggccactcgc gaacagagcc acggcctggt cgcgagcctc
     1921 aaccgggacg atgtccacgc cttcgcgcgc tatgccgtca tcgcggccgc ggtcctgccg
     1981 ttccttccca atcgcaacat gggcccgctg gattcctgga acccgtttca gctgtggctc
     2041 gtggtcgtcc tcatcaccgg cttctcgttc gccggctata tcgccagccg gaccatcggc
     2101 gcgcgcaagg gggtgctggc gacggccatc atcggcggcg cctattcatc gacggccgtc
     2161 accgcttcgc tggcccggca gctgggccgg ggagcggccg gcccgttcgc ggcgggaatc
     2221 ctgctcgcga gcgcaatgat gtacattcgg gtcgtcatcc tgatggccat tctgtcgcct
     2281 tcgacggtgg cgcagttcct ggtgacggtc ggtcccgccg ccgccgtcgg aatggccatc
     2341 gcgctcctgg cctggtggcg cgccccgcag gcggacgagg cgagggcgcc ggtgtccgga
     2401 aacccggtcg agcttctgcc cgcgttcggg ttcgtcgcga tcgtagccgc cgcggcggtg
     2461 gccacgcgat gggctcgcga gcggttcggc gagaccggta tcgcggcctc gctgctcttc
     2521 accggcagct tcgatgtcga cgccgcgatc gtcacgctct cgggcctgcc cgtctcggcg
     2581 atcgatcgcc gggtggcggc catcgccatt gccgggacca tcgtcgccaa catggccgtg
     2641 aagatcctcg tgacggctgt ctatgcgcgc cgctcgggcg ccgccgcggt ggcgggaatg
     2701 gcggcgagcg tcgcggtgct ggtgctgacc atcggctggc gcctggcgat cgcctaggag
     2761 cttgtcacga gcgccccggc cggcgggccg gccgcttcgg tttccctccg cttgggcggg
     2821 gccggcacgg gatggagcgg cactccgctg cgctcgaagg aatcgcaatt ggcgatcgtg
     2881 gtccgggcga tgttggccag cgcctcgacc gtgaagaaac cctggtggcc ggtgaccagg
     2941 acgttgggga aggtcaggag cctcgcgaac tggtcgtcca tgatcgcctg gtccgaccgg
     3001 tcctcgaaga acagggcttc ctcctcctcg taaacgtcga ttgccagccc tcccagccgg
     3061 cggcgcttga gggcgccgat caccgcttgc gtgtcgacca ccgccccacg gctggtgttg
     3121 atcaggatcg cgcccggctt catgcgatcc agagcggacg aatcgatcag gtggcgggtg
     3181 gccgagttga gcggacagtg cagggtgacg atgtccgatt cggcgagaag ggtgtcgaga
     3241 tcgacatagt cgaccaggca cgcgcactcg gagcgccgaa ccgggtcgct cgccaatatc
     3301 ctgcagccga accccttggc gatcgtcgcc acgaccgccc cgatgttgcc gacgccgacg
     3361 attccgatcg tcttttcggc caggtcgaag ccgagcagcc cctcgagcgc gaaattgcct
     3421 tcccggaccc ggttgtaggc acgatggatc ttgcggacga gcgcgaggat cagggcgaag
     3481 ctgtgctcgg ccaccgagtg cggcgaataa gcggggacac ggcagaccgc gatccccttg
     3541 gagtgcgcgg cgctcaggtc gacattgttg aagccggccg agcgcagggc gatcagccgc
     3601 actccaagtt cggcgaatgt ctcgatcgcc gctcggtccg aacgatcatt ggcgaacagg
     3661 cagaccgcgt cggcgcccct ggcaagcggc gcgcttgcgc tcgtcagcct cggttcgaga
     3721 tagcgcagtt cgtgccggcc ggaattggcg gcatcgaaga accggcggtc atacgacttg
     3781 gtgctgaaca gggcgatctt cacggcccgt caccttcgcg gctccactcg gcccttcggc
     3841 gagctcatcc cattgccagg cgccggcccg ccattcggat ggcttcgaga gcatcgccac
     3901 tcgaaggcgg tacatgtcgg ccagctcatc gtgccggtgc cgcgcggcgt cgcatgccgc
     3961 cagtcgggcg gcctgctgtt cctccagcgc acgccttcga cagtaaaaca ggtcct
//
LOCUS       DASTZO010000225         5240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1142305,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000225 DASTZO010000000
VERSION     DASTZO010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142305"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VFU20_06315"
     CDS             complement(<1..884)
                     /locus_tag="VFU20_06315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5286107.1"
                     /translation="MTLEDARALDEADPLRHARDRFRLPADLIYLDGNSLGALPAATP
                     ERLAELVERQWGDQLIASWNRHGWIEAPARVAAKLAPLLGARPDELLIADSTSVSLFK
                     LLVAAVRARPGRSTILSARGNFPTDLYVAQGAAELLPGARLASVPEDDVERAIDADTA
                     VVLLTHVDYRSAARFDIARINAAAHAHGALTLWDLSHSAGALELQLDATGCDLAVGCG
                     YKYFNGGPGAPAFLFVAERLQGELRSPLPGWMGHAEPFAFEPDYRPAEGIARFLTGTP
                     SIVALAAFDAGLDSFAGL"
     gene            complement(886..1440)
                     /gene="dcd"
                     /locus_tag="VFU20_06320"
     CDS             complement(886..1440)
                     /gene="dcd"
                     /locus_tag="VFU20_06320"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEU5286108.1"
                     /translation="MTIQSDRWIREQALKHAMIEPFVEAQRREGCISYGLSSYGYDAR
                     VSNHFKIFTNVDSATVDPKDFAANSFVDREVDVCIIPPNSFALAHTVEYFRVPRDVMV
                     ICLGKSTYARCGIIVNVTPLEPGWEGHVTLEFSNTTPLPAKVYANEGACQFLFLKGNE
                     PCETSYADRAGKYMGQRGVTLPKL"
     gene            1573..3087
                     /locus_tag="VFU20_06325"
     CDS             1573..3087
                     /locus_tag="VFU20_06325"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU5286109.1"
                     /translation="MAEILRIADPAATAGSPALPQNVEAEAALLGALMIDNRLVEDIQ
                     TRLQPHHFFEPLHGRIYEAILRLTDRNMIANPVTLRPLFEADEGIKEVGGPAYLAQLT
                     GSGAAVIGARDFAAQIYDLALLRALIGVGRDLVESALDTSEEVAPIAQIERAETELYK
                     VAEEGGAEGKAKSFGEATKLAIEMAERALNNGGHLSGITTGLDSLNSKIGGLHRSDLI
                     IIAGRPGMGKSSLATNIAFAAAQRFLRDADDGVEPGKGAGAAVALFSLEMSADQLATR
                     ILAEQSGISSENLRMGKISKQEFRQLARAAGELQSLPLYIDDTPGLTIAALRARARRL
                     KRQKGIGMVIIDYLQLLQGTGRNSNENRVQEISEISRGLKQLAKELDLPVVGLSQLSR
                     AVESREDKRPQLSDLRESGSIEQDADIVLFIYREDYYVASREPKRPAEGDDAKVHEMH
                     EEWARDMNRVYGLAELIVAKQRHGATGKVRLKFDSRITKFSDAADDGYLPEIRS"
     gene            complement(3394..4836)
                     /gene="astD"
                     /locus_tag="VFU20_06330"
     CDS             complement(3394..4836)
                     /gene="astD"
                     /locus_tag="VFU20_06330"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HEU5286110.1"
                     /translation="MTKSETAKTLVSTDPATGAEIWSGPIGDPAAEVAAAREAAGAWA
                     AHSLAYRSEAVRRFANVVRKREEEFATLISTETGKPFWEARTEVAAVINKVQISIDAY
                     SERTPQRRLEAALGSKIAVRHKPHGVLAVLGPYNFPAHLPNGHIVPALIAGNAVVFKP
                     SEKTPATGAFLVECFHQAKIPEGVVRLLVGGPDQGRALAAEPGIDGLLFTGSARVGKA
                     LHKQFADTPHKILALELGGNNPLVVWDAKDVDAAAAIVVQSAYLSAGQRCTAARRLIV
                     EDGQHGPLVEAIQRLVDRIIVDQPFADPQPFMGPLIDLPSADHLQEQWLGLMMKGGKP
                     VRRLDRPVEDRPFLTPALIDVTAIADRPDEELFGPVLQLIRVEDFDAAIAEANNTRFG
                     LAASLVGGGPELYDRFWANIRAGVINWNKPTNGAPSNAPFGGVGLSGNHRPSAYYAAD
                     YCAYPVTSSEASVVRATIGEGLKDPNLLED"
     gene            complement(4870..>5240)
                     /locus_tag="VFU20_06335"
     CDS             complement(4870..>5240)
                     /locus_tag="VFU20_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="selenoprotein O"
                     /protein_id="HEU5286111.1"
                     /translation="LGVAPRSEEDDRSLAAALVRALQSRDAAIDRIFFDWRGGRDPGA
                     DRYPTEPFRALAAALQGRERPAAHPYWSDSGPCSMHIEEVEAIWSAIAERDDWAPFEA
                     KVAAVRRMGEAMAADMAAPA"
BASE COUNT          840 a         1765 c         1860 g          775 t
ORIGIN      
        1 tcgagcccgg cgaagctgtc gagcccggca tcgaacgcgg cgagcgcgac gatcgacggg
       61 gttccggtca ggaagcgcgc gatgccctcg gccgggcggt aatccggctc gaacgcgaac
      121 ggctcggcat gccccatcca ccccggcagc ggcgagcgca gctcgccctg cagccgctcg
      181 gcgacgaaca ggaatgccgg agcgcccggg ccgccgttga aatatttgta gccgcagccg
      241 acggcgaggt cgcagccggt cgcgtcgagc tggagctcga gcgcgccggc gctgtgcgac
      301 aggtcccaca gggtgagcgc gccatgcgcg tgcgcggccg cgttgattcg cgcgatgtcg
      361 aaacgggcgg cgcttcgata gtcgacatgg gtgagcagga cgacggcggt gtcggcgtcg
      421 atcgcgcgct cgacgtcgtc ctccgggaca ctcgccagcc gcgccccggg caacagctcg
      481 gccgccccct gggcgacata gaggtcggtc gggaaattgc cccgtgccga gaggatcgtc
      541 gagcgtccag ggcgggctcg aaccgcggcg accagcagtt tgaacagcga caccgaggtc
      601 gaatcggcga tcagcagctc gtccggccgc gctccgagca gcggcgcgag cttcgccgcg
      661 acccgggccg gggcctcgat ccagccatgg cggttccagc tggcgatcag ctggtcgccc
      721 cactggcgct cgaccagctc ggcgaggcgc tccggggtcg ccgccggcag cgcgcccagc
      781 gaatttccgt cgagatagat gaggtcggcg ggaagccgga agcggtcgcg ggcatggcgc
      841 agcgggtcgg cctcgtcgag cgcgcgcgcg tcctcgaggg tcacgctaca gcttcggcaa
      901 ggtgaccccg cgctggccca tatatttgcc ggcgcggtcg gcgtagctgg tctcgcacgg
      961 ctcgttgccc ttcaaaaaca ggaactggca cgcgccctcg ttggcgtaga ccttggccgg
     1021 cagcggcgtg gtgttggaga attcgagggt cacatggcct tcccagccgg gctcgagcgg
     1081 ggtcacgttg acgatgatcc cgcagcgcgc ataggtcgac ttgccgaggc agatcaccat
     1141 cacgtcgcgc ggcacccgga aatattcgac cgtgtgggcg agcgcgaagc tgttgggcgg
     1201 gatgatgcac acgtcgacct cgcggtcgac gaagctgttg gcggcgaagt ccttggggtc
     1261 gacggtcgcc gaatcgacgt tggtgaagat cttgaaatgg ttcgagaccc gcgcgtcgta
     1321 gccgtaggac gacaggccgt agctgatgca gccctcgcgc cgctgcgcct cgacgaacgg
     1381 ctcgatcatc gcatgcttaa gtgcctgctc gcgaatccag cggtccgatt ggatggtcat
     1441 cggctgctgg cctagccgtg cttgggcggg ccgcgcaatg ccactttcca tccacaggcc
     1501 agtccacgaa aaagtttgca agttgcgaac tttggatgga ggttgtgggg ccaaactcgg
     1561 taagccgaag ccatggcaga aatccttcga atcgccgacc ccgcggctac cgcgggaagc
     1621 ccagcgctcc cccagaacgt cgaggcggaa gccgctctgc tgggcgcgct gatgatcgac
     1681 aaccggctgg tcgaggacat ccagacgcgg ctccagccgc accatttctt cgagccgctg
     1741 cacggccgca tctacgaggc gatcctgcgc ctgaccgacc gcaacatgat cgccaacccg
     1801 gtgactctca ggccgctgtt cgaggccgac gaagggatca aggaggtcgg cggtccggcc
     1861 tatctcgcgc agctgaccgg ctcgggcgcg gcggtgatcg gggcgcgcga cttcgccgcc
     1921 cagatctacg atctggcgct gcttcgggcg ctgatcgggg tcggccgcga cctcgtcgag
     1981 agcgcgctcg acaccagcga ggaagtcgcc ccgatcgccc agatcgaaag ggccgagacc
     2041 gagctctaca aggtcgccga ggaaggcggc gccgaaggca aggcgaaatc gttcggcgag
     2101 gccaccaagc tggcgatcga gatggccgag cgggcgctca acaacggcgg ccatctgtcg
     2161 gggatcacca ccggcctcga cagcctcaac tcgaagatcg gcggcctgca ccgctccgac
     2221 ctgatcatca tcgccggccg ccccggcatg ggcaagagct cgctcgcgac caacatcgcg
     2281 ttcgccgccg cgcagcgctt cctgcgcgac gccgacgacg gggtcgagcc gggcaagggg
     2341 gcgggcgcgg cggtggccct gttcagtctc gaaatgtcgg ccgaccagct ggcgacccga
     2401 atcctcgccg aacagtcggg catcagctcc gaaaatttgc gcatggggaa gatcagcaag
     2461 caggagttcc gccagctcgc gcgcgccgcg ggcgaattgc agagcctgcc gctgtacatc
     2521 gacgatacgc ccggcctcac catcgccgcg cttcgcgccc gcgcccggcg cctgaagcgc
     2581 cagaagggca tcggcatggt gatcatcgat tatctgcagc tgctccaggg caccggcagg
     2641 aattcgaacg agaaccgggt ccaggaaatc tcggagatca gccgcggcct caagcaattg
     2701 gccaaggagc tcgacctgcc ggtggtcggc ctgtcgcaat tgagccgcgc ggtcgagagc
     2761 cgtgaggaca agcgtcccca actctccgac ctgcgcgagt ccggatcgat cgagcaggac
     2821 gcggacatcg tcctgttcat ctaccgggag gattattacg tcgcctcgcg cgagcccaag
     2881 cggccggccg agggcgacga cgccaaggtc cacgagatgc acgaggaatg ggcgcgggac
     2941 atgaaccggg tctacggcct cgccgagctg atcgtcgcca agcagcgcca cggcgcgacc
     3001 ggcaaggtgc gcctcaagtt cgacagccgg atcaccaagt tcagcgacgc ggccgacgac
     3061 ggctatctgc cggagatccg cagctaggcc gagatttatg gatggggcgg gggcgccgcc
     3121 tgtccggatg ggcaggcggc gttagctcag ccgggaccgg cgccgatttg cgcgagccgc
     3181 cggcggatgc cgcggtagga tggggagagt gcgagccgca attggtggtg cagccgctcg
     3241 accatcgaga acgccaggga catgggatcc tccgttcgtt cgccagtgcg cgccggttga
     3301 atcacggctc ggccgcccga tcaacacgaa gcgggggcgg gcccgacttt ccctgccgtc
     3361 ctgtgtcgtt tcggactcac atcgaaatcg cggtcagtcc tcgagcagat tggggtcctt
     3421 cagcccctcg ccgatcgtgg cccggaccac cgacgcttcg ctgctggtca ccgggtacgc
     3481 gcaataatcg gccgcgtaat aagcgctcgg ccggtggttg ccggacaggc cgacgccgcc
     3541 gaacggcgcg ttcgacggcg cgccgttggt cggcttgttc cagttgatca ccccggcgcg
     3601 gatattggcc cagaagcggt cgtagagctc cggcccgccg ccgaccaggc tcgccgcgag
     3661 cccgaagcgg gtgttgttcg cctcggcgat cgcggcgtcg aaatcctcga cccggatcag
     3721 ctgcaggacc gggccgaaca attcctcgtc ggggcggtcg gcgatggcgg tgacgtcgat
     3781 cagcgccggg gtcaggaacg gccggtcctc gaccgggcgg tcgagccgcc gcaccggctt
     3841 gccgcccttc atcatcagcc ccagccattg ctcctggaga tggtcggccg acggcaggtc
     3901 gatcagcggg cccatgaacg gctgcgggtc ggcgaacggc tggtcgacga tgatccggtc
     3961 gacaagccgc tggatggcct cgacgagcgg gccgtgctgg ccgtcctcga cgatcagccg
     4021 gcgggccgcg gtgcagcgct ggccggccga cagataggcc gactggacga cgatcgccgc
     4081 cgctgcgtcg acgtccttgg cgtcccacac caccagcggg ttgttgccgc cgagctcgag
     4141 cgcgaggatc ttgtggggcg tgtcggcgaa ttgcttgtga agcgccttgc cgacgcgcgc
     4201 cgagccggtg aacagcaggc cgtcgatgcc gggctcggcg gcgagcgccc ggccctggtc
     4261 gggaccgccg accagcagcc ggacgacgcc ttcgggaatc ttcgcctggt ggaagcattc
     4321 gacgaggaag gcgccggtcg ccggggtctt ctcggacggc ttgaagacca ccgcgttgcc
     4381 ggcgatcagc gccgggacga tgtggccgtt gggcaggtgg gccgggaagt tgtacgggcc
     4441 gagcaccgcc agcaccccgt gcggcttgtg gcggaccgcg atcttgctgc cgagcgccgc
     4501 ctcgagcctg cgctgcggag tgcgctccga ataagcgtcg atcgagatct ggaccttgtt
     4561 gatgaccgcc gcgacctcgg tccgggcctc ccagaacggc ttgccggtct cggtcgagat
     4621 cagggtcgcg aactcttcct cgcgcttgcg cacgacattg gcgaagcggc gcaccgcctc
     4681 ggagcgatag gcgagcgaat gcgcggccca ggcgccggcc gcctcccgcg ccgcggcgac
     4741 ctcggcggcc gggtcgccga tcgggcccga ccagatttcc gcgccggtcg ccgggtcggt
     4801 cgagaccagg gtcttggccg tttcgctttt ggtcattgtg ccgcgcgctt cacgaacttg
     4861 ggcgcggcgt caagccggtg cggccatgtc cgcggccatc gcctcgccca tccggcggac
     4921 cgcggcgacc ttggcctcga acggcgccca gtcgtcgcgc tcggcgatcg ccgaccagat
     4981 cgcctcgacc tcctcgatat gcatcgagca gggcccggaa tccgaccagt aggggtgggc
     5041 ggccgggcgc tcgcggccct gcagggcggc ggccagcgcc cggaacggct cggtcggata
     5101 ccggtccgcg cccgggtcgc ggccgccgcg ccaatcgaag aagatgcggt cgatggcggc
     5161 gtcgcgcgac tggagcgccc ggacgagtgc ggcggcgagg ctgcggtcgt cctcctccga
     5221 gcgcggcgcc accccgagcc
//
LOCUS       DASTZO010000226         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_933674,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000226 DASTZO010000000
VERSION     DASTZO010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933674"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /gene="rpsO"
                     /locus_tag="VFU20_06340"
     CDS             complement(<1..267)
                     /gene="rpsO"
                     /locus_tag="VFU20_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU5286112.1"
                     /translation="MSITAERKAELIKEHGRGSDDTGSPEVQVAIITDRINTLTEHFK
                     THAKDNHSRRGLLMMVNKRRSLLDYLRREDEKRYTDLIAKLGLRK"
     gene            complement(285..1166)
                     /gene="truB"
                     /locus_tag="VFU20_06345"
     CDS             complement(285..1166)
                     /gene="truB"
                     /locus_tag="VFU20_06345"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU5286113.1"
                     /translation="MHGWIILDKPVGLGSTQAVGAVKRILREAGEPRTKVGHGGTLDP
                     LASGVLPIALGEATKLAGRMLDATKEYEFTVGFGEETDTLDAEGRVVETSDRRPARAE
                     IEAVLPRFTGAIEQVPPAFSALKIEGQPAYKRSRAGEQLDMAARDVTVHELRLLESSP
                     DEATFAASVSKGTYVRSLARDIARALGTVGHVTMLRRTRAGAFGLEMAISLDFLEAAA
                     KARRLDGAVLPLTAALDDIPALPVTPGQAQLLRHGQKLAGFPATPGLALATDDDRPVA
                     LVEASAGGLRVVRGFNL"
     gene            complement(1166..1780)
                     /locus_tag="VFU20_06350"
     CDS             complement(1166..1780)
                     /locus_tag="VFU20_06350"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU5286114.1"
                     /translation="MAKLYFYYAAMNAGKSTTLLQADFNYRERGMATMLWTAAHDDRA
                     GSGLVGSRLGLTIAAHAYSPDIDLFDAVGEELKRRELHCVLVDEAQFLTRGQVLQLCR
                     LCDEVNLPVLCYGLRTDFLGQLFEGSAALLALADALVELKAVCECGRKATMNLRVDAE
                     GHAVAAGEQTEIGGNDRYIALCRRHFFERLKEGEARQLSLDIPR"
     gene            complement(1807..2337)
                     /locus_tag="VFU20_06355"
     CDS             complement(1807..2337)
                     /locus_tag="VFU20_06355"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HEU5286115.1"
                     /translation="MKPLAEPMTGEDCRAEPLTEAHREPLRAACAEDPDIWDIYYASY
                     APAEFDSSFDALLKRPWVPFALYDGDAFVGMSCFLNIDEGRQALEIGSTYYRPSHRGT
                     GFNRRCKAMMLAHAFERGCRRVEFRVDSRNTRSQAAMKKLGAVREGVLRGDRITWNGH
                     VRDTVLFAILKDEWPV"
BASE COUNT          360 a          827 c          808 g          385 t
ORIGIN      
        1 cttgcgcaga cccagcttgg cgatgaggtc ggtgtagcgt ttctcatcct cgcggcggag
       61 ataatcgagc agcgaccgcc gcttgttgac catcatcagc aggccgcggc gcgaatggtt
      121 gtccttcgcg tgggtcttga aatgctcggt cagagtgttg atccggtcgg tgatgatcgc
      181 gacctggacc tcgggcgaac cggtgtcgtc ggacccgcgg ccatgctcct tgatcagctc
      241 ggccttgcgc tcggcagtga tcgacatcgt caaactcctc atcttcaaag gttgaacccg
      301 cgaacgaccc gaaggccgcc tgccgaagct tccaccagcg caaccggacg atcgtcgtcg
      361 gtggcgagcg caagccccgg cgtcgcgggg aaaccggcaa gcttctgtcc gtgtcggagc
      421 agctgggcct ggccgggggt gacggggagg gccgggatgt cgtccagcgc cgcggttagc
      481 gggagaaccg ccccatcaag ccgccgcgcc ttagcggctg cctcgagaaa gtccagcgaa
      541 atcgccattt ccaggccgaa cgcgccggcg cgcgtgcgcc tgagcatggt cacatgcccg
      601 accgtcccga gcgcccgggc aatgtcgcgg gcgaggctcc ggacgtaggt gcccttggac
      661 acggaggcgg cgaacgtcgc ctcgtccggg ctgctctcga gcagccgcag ctcgtgcacc
      721 gtcacatcgc gcgcggccat gtcgagctgc tcgccggccc gcgagcgctt gtatgccggt
      781 tgcccttcga tcttcagcgc ggaaaaggcc ggcggcacct gctcgatcgc gccggtgaag
      841 cgcggcagca ccgcctcgat ctccgcgcgc gccggacggc ggtcgctggt ctccaccacc
      901 cgcccctccg cgtcgagcgt gtcggtctcc tcgccgaagc cgaccgtgaa ctcatactcc
      961 ttggtcgcat cgagcatccg gccggcgagc ttggtcgcct cgccgagcgc gatcggcagc
     1021 accccgctgg ccagcgggtc gagcgtcccg ccgtggccga cctttgtcct gggctcgccg
     1081 gcttcgcgca ggatccgctt gaccgccccg accgcctggg tcgagccgag cccgaccggc
     1141 ttgtcgagga tgatccagcc gtggatcacc gcgggatatc aaggctcagc tggcgggctt
     1201 cgccttcctt cagccgctcg aagaaatggc ggcggcagag cgcgatgtaa cggtcgttgc
     1261 cgccgatctc ggtctgctcg ccggccgcga ccgcgtggcc ctcggcatcg acgcgcaggt
     1321 tcatcgtcgc cttgcggccg cattcgcaca ccgccttcag ctcgacgagc gcgtcggcga
     1381 gcgcgagcag cgccgcgctg ccctcgaaca actggccgag gaagtcggtc ctgaggccgt
     1441 agcagagcac cggcagattg acctcgtcgc acagccggca cagctgcagc acctggccgc
     1501 gggtcaggaa ctgcgcctcg tcgaccagca cgcagtggag ctcgcggcgc ttcaattcct
     1561 cgccgaccgc gtcgaacagg tcgatgtcgg gcgaataagc gtgcgccgcg atggtcaggc
     1621 cgagccgcga gccgaccagc cccgacccgg cgcggtcgtc gtgggccgcg gtccacagca
     1681 tggtcgccat gccgcgctcg cgatagttga agtcggcctg caggagcgtg gtcgatttcc
     1741 cggcgttcat cgccgcatag tagaaataga gcttggccat cgaggaagcc gattagcggc
     1801 tcggcgtcac accggccact catccttgag gattgcgaac agcacggtgt cgcggacgtg
     1861 gccgttccag gtgatgcggt cgccgcgcag cacgccctcg cgcaccgctc ccagcttctt
     1921 catcgccgcc tggctgcgcg tgttgcgcga atcgacccgg aactcgaccc ggcggcagcc
     1981 gcgctcgaac gcatgggcca gcatcatggc cttgcagcgg cggttgaagc ccgtgccgcg
     2041 atgcgagggg cgatagtaag tgctgccgat ctcgagcgcc tggcggccct cgtcgatgtt
     2101 gaggaagcag ctcatgccga cgaacgcgtc gccgtcgtag agggcgaacg gcacccacgg
     2161 ccgcttcagg agcgcgtcga agctcgagtc gaactccgcg ggcgcgtagc tggcgtaata
     2221 gatgtcccag atgtccgggt cctcggcgca ggcggcgcga agcggctcgc ggtgggcctc
     2281 ggtcagcggc tcggcccggc aatcctcgcc cgtcatgggc tcggcgagcg gcttcattcg
     2341 cgctcgaggt cctgcgccac cttggggtcg cgcagcaacc
//
LOCUS       DASTZO010000227         4412 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_489913,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000227 DASTZO010000000
VERSION     DASTZO010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_489913"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="VFU20_06360"
     CDS             <1..318
                     /locus_tag="VFU20_06360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU5286116.1"
                     /translation="LSEFGKLLASRLRRADVVARYGGEEFVAVLAGTDRHDAVRVADQ
                     IRAAFEKMAFPTEDGESVRCTVSAGVATIPAGDPSLATLVPTADVALSMAKRAGRNMV
                     AAA"
     gene            382..756
                     /locus_tag="VFU20_06365"
     CDS             382..756
                     /locus_tag="VFU20_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5286117.1"
                     /translation="MSTRPILVLALLLAACAPPAAATVAVPVTVTAGPVCPVVTDPPD
                     PSCDDRPVAGAELIVRGPDGRTVTTVRSDDAGHATLQLAPGSYVLVPQPVEGLMGTAE
                     EMTIVVTDDPEPITVGYDTGIR"
     gene            complement(877..1080)
                     /locus_tag="VFU20_06370"
     CDS             complement(877..1080)
                     /locus_tag="VFU20_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286118.1"
                     /translation="MRAYYLIPLLVAVAAVAYWLLAYEAAGAVMLLIFAGAMALMGWI
                     LLPTLGDVGHTAPIDPDFEDPGR"
     gene            complement(1159..1713)
                     /locus_tag="VFU20_06375"
     CDS             complement(1159..1713)
                     /locus_tag="VFU20_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286119.1"
                     /translation="MINRAGDPRTDDTIVYELLRTPRGGDAIAVGRATFRAGRSDVDA
                     PDEVAIAVRELLERGFVDRIQSDERPRGYRRSGSGRVEMLVPGMAEHFIARLRGLWLP
                     YPDGSVVTAREAGIVGTVPAPSLAVPDGGETGPAITDAAIRRSSLAEADHLLDKRPIV
                     RANPPAPGLRAAPSVRRTDCGWIT"
     gene            1800..2156
                     /locus_tag="VFU20_06380"
     CDS             1800..2156
                     /locus_tag="VFU20_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286120.1"
                     /translation="MSESDAATYRAMVTEQSDDQIDRWSGDLFIDFAKRMGVGTAISA
                     FCRAAGLDERGFQRAFLVGGGPDHVVGIDTAGALAAPIFELPKAVAGLRRIDPQARAK
                     LIDFLVDQREVMAYTP"
     gene            complement(2207..3187)
                     /locus_tag="VFU20_06385"
     CDS             complement(2207..3187)
                     /locus_tag="VFU20_06385"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU5286121.1"
                     /translation="MGSSSKRPAFRENIHRLFDEAQSADLAPGIVSLIESRATRAQLR
                     EVGIDKILPNPAQPRLSYEEDSLTELADSIREHGVLQPILVRTVGNQYELIAGERRWR
                     ASRMAERETIPAIVVEFDEQTALEVSIIENLQREDVSPLEEASMFRKMTDLGYSVRQL
                     AQKIGKDKGYVENRIRLAEAPPEIRELVSLRKDTLSHAYELMKIGDERTRRRLAKKVA
                     AGELTLAKLRAITGGEESDHPEGRPKRRARTVAGEVAVAKATDNALLDTKNRLADSVD
                     ELVDLLRQREVIDQIPAVNRDNLAKYLTITRLKIENAIAIIRSSPAPEEP"
     gene            complement(3180..3947)
                     /locus_tag="VFU20_06390"
     CDS             complement(3180..3947)
                     /locus_tag="VFU20_06390"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5286122.1"
                     /translation="MTKVISIANQKGGVGKTTTSINLGGALAELGYRVLCIDMDPQAN
                     LTVGLGISLSDVKASMSDVLSEDRSGLEEIVKQTEMSGLSVAPATLELASTEVELFTA
                     IGREMVLRDALEGWVERQFDVVIIDCPPTLGLLTINALVASNRVIIPVQTQYYAIKGL
                     TALIKVINTIKLKLNHDLEILGLLATFYDGRTVLAREMLENLRELGDHRVFNTMIKNT
                     VKLGEAPLTGKPVTEYATNSAAARAYRDLAREVVELG"
     gene            4153..4228
                     /locus_tag="VFU20_06395"
     tRNA            4153..4228
                     /locus_tag="VFU20_06395"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4186..4188,aa:Ile,seq:cat)
BASE COUNT          617 a         1516 c         1568 g          711 t
ORIGIN      
        1 ctgagcgagt tcggcaagct gctggcctcg cgcctgcgac gcgctgacgt ggtggcccgc
       61 tacggcggcg aggaattcgt tgcggtgctg gccggcaccg atcggcacga cgcggtccgc
      121 gtggcagacc agatccgtgc cgcgttcgag aagatggcct tccccaccga ggacggcgaa
      181 tcggtccgtt gcacggtgag cgccggtgtc gccaccatcc cggccggcga tccgtcgctg
      241 gcgaccctgg tgcctaccgc cgatgtcgcc ctgtcgatgg ccaagcgcgc cggtcggaac
      301 atggttgcgg ccgcctagct tcgccatgcg cgcgtgcccg aggctgtgca accgttcggc
      361 gccccgcggc gtcccgggcg catgagcacc cgaccgatcc ttgtcctggc gctgctgctc
      421 gccgcctgcg cgccgccggc cgcagccacc gtcgccgtac cggtcaccgt gaccgccggc
      481 ccggtctgcc cggtcgtcac cgacccgccc gacccgagct gcgacgatcg accggtcgcc
      541 ggggccgagc tgatcgtccg cggccccgac gggcgcacgg tgacgaccgt ccggagcgac
      601 gatgccggcc acgccacgct ccagctggcg ccgggcagct acgtcctggt cccacagccg
      661 gtggaggggc tgatgggcac ggccgaggag atgacgatcg tcgtgaccga tgatcccgag
      721 ccgatcacgg ttggctacga cacgggtatc cggtgattga gcgccgagcc ggctaacggc
      781 ggcgcacctg gtccgttagt actactgaac gccggttccc tgtgttagcg gctcagccgc
      841 tagggcagcc acagggatcc ggcgttttcg ctgtccctag cggcccgggt cctcgaaatc
      901 gggatcgatc ggggcggtgt ggcccacgtc gccgagggtg gggagcagga tccatcccat
      961 cagcgccatg gcgccggcga agatgaggag catgaccgcc ccggccgcct cgtacgccag
     1021 cagccagtag gcgacggcgg ccaccgcgac gagcaggggg atgaggtaat aggcgcgcat
     1081 ggctggatga tagccgagca ggcggacccg gtgccggcgc ggcagcgcgg ccgtatatct
     1141 acgtgaacct ggtaatacct aggtgatcca tccgcagtcg gtgcgcctga ccgatggcgc
     1201 tgcgcgcagg ccgggcgccg gcgggttggc ccgcacgatg ggccgcttgt cgagcaggtg
     1261 gtccgcctcg gcgaggctgg aacgacggat ggcggcatcg gtgatcgcag ggccggtctc
     1321 cccgccgtcg gggacggcca gggacggggc aggcaccgtc cccacgatgc cggcttcgcg
     1381 ggcggtgacc accgagccgt cggggtaggg gagccacagg ccgcgcaggc gggcgatgaa
     1441 gtgctcggcc atgccgggga ccagcatctc gacgcggcca ctgccgctgc ggcggtaccc
     1501 gcgcgggcgc tcgtcggact ggatgcggtc gacgaacccg cgctcgagaa gctcacggac
     1561 cgcgatcgcc acctcgtccg gggcgtcgac gtcgctgcgt ccggcgcgga aggtcgcccg
     1621 cccgacggcg atcgcgtcgc ctccccgcgg ggtccggagc agctcgtaga cgatcgtgtc
     1681 gtcggtccgc ggatcgcccg cgcggttgat catggccgga ggatagcagc ccgtcggcgg
     1741 ccgccgacgg gccccgtcgc gcatcgctag aatcggcgcg agcagcactg gagaccccct
     1801 tgagcgagag cgacgcggcc acctaccgcg ccatggtgac cgagcagagc gacgaccaga
     1861 tcgaccgttg gtcgggtgac ctgttcatcg atttcgccaa gcggatgggc gtcggaacgg
     1921 ccatctccgc cttctgccgt gccgcgggcc tggacgagcg cggcttccag cgggcgttcc
     1981 tggtcggagg cggcccggac cacgtcgtcg ggatcgacac cgcgggcgcc ctggccgcgc
     2041 cgatcttcga gctgccgaag gcggtcgccg ggctgcgcag gatcgacccg caggcgcgtg
     2101 ccaagctcat cgacttcctc gtcgaccagc gcgaggtgat ggcctacacg ccgtaggtcg
     2161 cgcgccaccc ggccgccggg ggcggccgca tcggtccggt gggggactac ggctcctcgg
     2221 gagccgggct ggagcggatg atcgcgatcg cgttctcgat cttgagccgc gtgatggtca
     2281 ggtacttggc caggttgtcg cggttgaccg ccgggatctg gtcgatcacc tcgcgctgcc
     2341 gcagcaggtc caccagctcg tcgaccgagt cggcgaggcg gttcttggtg tcgagcagcg
     2401 cgttgtcggt ggccttcgcc acggccacct cgccggccac cgtcctggcg cgccgcttcg
     2461 gccggccctc ggggtggtcg ctctcctcgc caccggtgat ggcacgcagc ttggccagcg
     2521 tcagctcgcc cgccgcgacc ttcttggcca gccggcgccg ggtccgctcg tcgccgatct
     2581 tcatcagctc gtaggcgtgc gagagcgtgt ccttgcgcaa agacaccagt tcgcggatct
     2641 cgggcggcgc ctccgccagc cggatgcggt tctcgacgta gcccttgtcc ttgccgatct
     2701 tctgcgccag ctgccggacg ctgtagccga ggtccgtcat cttgcggaac atcgaggcct
     2761 cctcgagcgg cgagacatcc tcgcgctgga ggttctcgat gatgctcacc tcgagggccg
     2821 tctgctcgtc gaactccacg acgatggccg ggatcgtctc gcgctccgcc atgcgcgacg
     2881 cccgccagcg ccgctcgccg gcgatgagct cgtactggtt gccgaccgtg cggaccagga
     2941 tcggctgcag caccccgtgc tcgcggatgc tgtcggcgag ctcggtgagc gagtcctcct
     3001 cgtacgagag tcgcggctgg gcggggttgg gcaggatctt gtcgatgccg acctcgcgga
     3061 gctgcgcgcg ggtggcccgc gactcgatca gcgagacgat gcccggtgcc aggtcggccg
     3121 actgcgcctc gtcgaagagg cgatggatgt tctcgcggaa cgccggccgc ttgctgctgc
     3181 tacccaagct ccaccacctc tcgtgccagg tcgcgatacg cgcgcgcggc ggccgagttg
     3241 gtcgcgtact cggtcaccgg cttcccggtg agcggcgcct cgccgagctt caccgtgttc
     3301 ttgatcatgg tgttgaagac ccgatggtca cccagctcgc gcaggttctc gagcatctcg
     3361 cgggcgagca ccgtgcgccc gtcgtagaag gtggccagca ggccgaggat ctcgaggtcg
     3421 tggttcagct tgagcttgat ggtgttgatc accttgatca gcgcggtcag gcccttgatc
     3481 gcgtagtact gcgtctggac cgggatgatc acccggttgc tggccaccag cgcgttgatg
     3541 gtcagcaggc ccagcgtcgg tgggcagtcg atgatgacga cgtcgaactg gcgctcgacc
     3601 catccctcca gcgcgtcgcg caggaccatc tcacggccga tggcggtgaa cagctcgacc
     3661 tcggtgctcg ccagctccag cgtcgccggg gcgaccgaca gtcccgacat ctcggtctgc
     3721 ttgacgatct cctcgagacc gctgcggtcc tcggagagga cgtcggacat ggaggccttg
     3781 acgtcggaga gcgagatccc caggccgacc gtcaggttcg cctgcgggtc catgtcgatg
     3841 cacaggacgc ggtagcccag ctcggcgagc gccccgccca agttgatgga ggtggtcgtc
     3901 ttgccgacgc cgcccttctg gttcgcgatg ctgattacct tggtcaagtc acgacacctt
     3961 cgcgtctgcg ggcggcgggt ggagccttcg cgccgcgatg ccagatcggt ggatcggtcc
     4021 gtcgcggctg cgccgatgat agccgccggc ggtccggcgg ggccagccga gttatccaca
     4081 atgtggctcg atgtcgatgt ctttgtgcaa agacgagccg atatactcgg acttgtccac
     4141 cgtcctcttc gcgggcccat agctcagtgg tcagagcatc cggctcataa ccggctggtc
     4201 gcaggttcga tccctgctgg gcccaccacc gacgtatccc gaggagccca tcagaccgat
     4261 gtcgaccgcc gacaagctgg cgcgcctgcg cgccatccgc gacgaggcgc tgctcggcgg
     4321 gggagcgaaa cggatcgaac aacagcacgc gcgcggcaag ctgacggcgc gcgagcgcgt
     4381 ggagctgctc gtcgacgagg gctcgttccg gg
//
LOCUS       DASTZO010000228         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_328117,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000228 DASTZO010000000
VERSION     DASTZO010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_328117"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /gene="hslU"
                     /locus_tag="VFU20_06400"
     CDS             <1..931
                     /gene="hslU"
                     /locus_tag="VFU20_06400"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEU5286123.1"
                     /translation="AAEEAAMDRLLDALTGKGSSEATRASFRARFADGSLATAEVEIE
                     VEEAPSMPFEIPGMGAQMFDLKSMMGKALGQTPRARRKLKVPDAFARLTEEEADRRLD
                     PDDVNRAALADAEANGIVFLDEIDKIAVSDVRGGSVSREGVQRDLLPLIEGTTVATKY
                     GPIKTDHMLFIASGAFHMAKPADLLPELQGRLPIRVELKALTQADFVRILSATRASLT
                     EQYRALLATEGVTIDFTDEGIEALARIAAEVNEAVENIGARRLQTVMEKLLEDISFEA
                     EDRGGETLTIDAAFVDRQLSGIAKNADLSRYVL"
     gene            complement(928..1638)
                     /locus_tag="VFU20_06405"
     CDS             complement(928..1638)
                     /locus_tag="VFU20_06405"
                     /inference="COORDINATES: protein motif:HMM:TIGR02117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02117 family protein"
                     /protein_id="HEU5286124.1"
                     /translation="MPRRRRRSLAKRAAGWALLALAAVPALYLLAALAGSLIPVNRGW
                     REPDQGITIYLADNGIHADIIMPARLGNLDWEPLVPKRHFAAAHPQARWVAFGSGEER
                     VYLETPRWRDIKPKTVWSALTGGKRVMHVEWVRDPGYAAREIRLRPEEYRRLWAAIRA
                     DFELDAGGRTRRIDAPGYGCCDAFYWATGKANAIRTCNSWAANRLRLAGIKTSAWPPF
                     VQGLVWRYRKSSREGGGA"
     gene            1797..>3170
                     /locus_tag="VFU20_06410"
     CDS             1797..>3170
                     /locus_tag="VFU20_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHYT domain-containing protein"
                     /protein_id="HEU5286125.1"
                     /translation="MAGSRVVLRIISCIVYEHDLGLVLVAAAICLLAAFTTFATLDHA
                     RRGGRRETAWIALAAFVSAIGIWSTHFVAMLAYEPAIPVNYDLDETLLSVLAAVVIGG
                     LGWWTFERNRAGASVAGGFLIGASIVTMHYIGMSAMSMAGHIKWDSRLVASSVILAVA
                     FSIAAVADYRRRPQFIAWRPALLFAAAICSLHFLAMSAAVIHPDSSEIAAKAIESTTL
                     AIIIACATLLILFISLVAVLFQRKLVRLEMREAQRLKVMEDAVLAGDRQRAQLTCELK
                     QQADISSAALNSMAQGLSMYDENDRLVTFNRRYAELYDMPVALLACGTSLADVLAFRI
                     SNGSLPKGTLEHHLDETSNSQHAPGRSEIRLCNGRIIDIQRRPLPGGGWVATREDITE
                     RRRAAERSEYLASHDDLTGLPNRASFGEELKAVAQLGGDKLHALLLVDLDRFKEVNDT
                     LGHPIGDE"
BASE COUNT          514 a         1088 c         1053 g          515 t
ORIGIN      
        1 cgcggccgag gaagcggcga tggaccggtt gctcgatgct ctcaccggca aggggtcgag
       61 cgaggcgacc cgggccagct tccgtgcacg tttcgccgac ggcagcctcg ccaccgccga
      121 agtcgagatc gaggtcgagg aagccccgtc catgccgttc gagattcccg gcatgggcgc
      181 gcaaatgttc gatctgaagt cgatgatggg caaggcgctc ggccaaaccc ccagggcgag
      241 gcgcaagctg aaggtgcccg acgcgttcgc ccgcctgacc gaggaggagg ccgaccgccg
      301 cctcgacccc gacgacgtca accgcgcggc tctggccgac gccgaggcca acggcatcgt
      361 cttcctcgac gagatcgaca agatcgcggt cagcgacgtg cgcggcggct cggtcagccg
      421 cgagggcgtc cagcgcgacc tgctgccgct gatcgagggg accaccgtcg ccaccaaata
      481 cgggccgatc aagaccgacc acatgctgtt catcgcctcg ggcgcgttcc acatggccaa
      541 gcccgccgac ctgcttcccg agcttcaggg ccggcttccg atccgggtcg agctgaaggc
      601 gctgacccag gccgatttcg tccgaatcct gtcggcgacc cgggccagcc tgaccgagca
      661 gtaccgggct ttgctggcga ccgagggcgt gaccatcgac ttcaccgacg agggcatcga
      721 ggcgctcgcc cggatcgccg ccgaggtcaa cgaggcggtc gagaacatcg gcgcccgccg
      781 gctccagacg gtgatggaga aattgctcga ggacatcagc ttcgaggccg aggaccgcgg
      841 cggcgagacc ctgaccatcg acgccgcctt cgtcgaccgg caattgtccg gaatcgccaa
      901 gaacgccgac ctgtcgaggt acgtgctcta ggctcctccg ccctcccggg aggatttccg
      961 ataccgccac accagcccct ggacgaacgg cggccacgcg ctggtcttga tgccggcgag
     1021 gcgcaggcgg ttggcggccc agctgttgca ggtgcggatc gcgttcgcct tgccggtcgc
     1081 ccagtagaag gcatcgcagc agccgtagcc cggcgcgtcg atgcgccgcg tgcgcccgcc
     1141 ggcatcgagc tcgaagtcgg cgcggatcgc cgcccacagc cgccggtact cctcgggcct
     1201 cagccggatc tcgcgcgccg cgtagcccgg atcgcggacc cactcgacgt gcatcacccg
     1261 tttgccgccg gtcagcgccg accacaccgt cttcggcttg atgtcgcgcc agcgcggggt
     1321 ctcgagatag acccgctcct cgcccgaccc gaacgccacc cagcgcgctt gcggatgcgc
     1381 cgcggcgaaa tggcgcttgg gcaccagcgg ttcccagtcg aggttcccga gcctagccgg
     1441 catgatgatg tcggcgtgga tgccgttgtc ggcgagatag atggtgatgc cttggtcggg
     1501 ctcccgccag ccgcggttga ccgggatcag cgagcccgcc agcgcggcga gcaggtacag
     1561 ggccgggacc gccgcgagcg cgagcagcgc ccagccggcg gcgcgcttgg caagggatcg
     1621 tcgtctgcgg cgcggcattg cggcgattgt tggcacggag cgggtggctt gtctgcaccg
     1681 gcgatctcgg cgccgcttcg gtcatcggga tcggcgaacg gtcgagtgca gctccatccg
     1741 ttccggcgga atcggtaaca aagccttcaa gctcaacggg tcaaggctcg cgccggatgg
     1801 ccgggagccg agtggtgctg cggataatct cctgcatcgt ttacgaacat gatctcgggc
     1861 tggtgctggt cgcggccgca atctgccttc tggcggcgtt cacgaccttc gccactttgg
     1921 atcatgcgcg acgcggcggc cgccgcgaga ccgcctggat tgcgcttgcc gcattcgtct
     1981 ccgcgatcgg catctggtcg acgcattttg tcgccatgct ggcgtacgag ccggcaatcc
     2041 cggtcaatta tgatctggat gagaccttgc tctcggtgct ggccgcggtc gtcatcggcg
     2101 gactgggctg gtggacgttc gagcgcaatc gcgccggcgc ctccgtcgcc ggtggcttcc
     2161 tgatcggcgc aagcatcgtc accatgcatt atatcggcat gtcggcgatg agcatggccg
     2221 gccatatcaa gtgggattcg cggctggtcg caagttcggt gatcctcgcg gtggcgttca
     2281 gcatcgccgc ggtggcggac taccgccgcc gaccgcaatt catcgcctgg cgcccggcgc
     2341 tcctctttgc cgcggcgatc tgcagccttc acttcctcgc catgtcggcg gcggtcatcc
     2401 atcccgattc ttccgaaatc gccgccaagg cgattgaaag cacgacgctg gcgatcatca
     2461 tcgcctgcgc gacgctcttg atcctgttca tcagcctcgt cgccgtcctg ttccagcgca
     2521 agctggtgcg tctcgagatg cgcgaagccc agcggctcaa agtcatggaa gacgcggtgc
     2581 tggcaggcga ccggcagcgg gcgcaattga cctgcgagct caagcagcag gccgacatca
     2641 gcagcgccgc gctcaacagc atggcccagg gcctgtcgat gtatgacgaa aacgaccggc
     2701 tcgtcacctt caaccgccgc tatgccgagc tttacgacat gccggtcgcg ctcctcgcct
     2761 gcggcacgtc gctggccgac gttctggcct tccgcatcag caacggcagc ttgccgaagg
     2821 ggactctgga gcatcatctc gacgagacca gcaattcgca gcacgctccc ggccgaagcg
     2881 agatccggct gtgcaacggc cggatcatcg acatccagcg ccgcccgctg cccgggggcg
     2941 gctgggtcgc gacccgcgag gacatcacgg agcggcgccg ggcggcggag cgcagcgaat
     3001 atctggccag ccacgacgat ttgaccggcc ttcccaaccg ggcgtcgttc ggcgaggaac
     3061 tgaaggccgt tgcacagctc ggcggcgaca agcttcatgc cctgctgctg gtcgacctcg
     3121 atcggttcaa ggaagtcaac gacacgctcg gccatccgat cggcgacgag
//
LOCUS       DASTZO010000229         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_176118,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000229 DASTZO010000000
VERSION     DASTZO010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176118"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..829)
                     /locus_tag="VFU20_06415"
     CDS             complement(89..829)
                     /locus_tag="VFU20_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5286126.1"
                     /translation="MQESSLRGGPGHGPAPGPAIALTGVNLSLGRGAARVHILKNIDL
                     HIGAGEAIGLVGPSGSGKSTLLMVMAGLERADAGDVVVAGEALGRLGEDALARFRGRH
                     IGIVFQSFHLIPTMTALENIAVPLELAGIQDAHIRAERELAAVGLAERRDHYPAQLSG
                     GEQQRVALARALAPDPAILIADEPTGNLDETTGREIIDLLFAGHAERNTTLVLVTHDT
                     ALARRCDRVVRLRSGRIEHEPRREAQPS"
     gene            924..1565
                     /locus_tag="VFU20_06420"
     CDS             924..1565
                     /locus_tag="VFU20_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HEU5286127.1"
                     /translation="MARYAGVPLLVLLVAVAAAPAAERPVKIVALGDSLTAGLGLAAS
                     EAFPAKLEKALRARGLAVEIANAGVSGDTASGGLARLDWAVPADTDAVIVELGANDML
                     RGTDPQIARRALEEIVRRLTDRRIAVLLAGMRAAPNLGPEYARQFEAIYPELAAKYDV
                     VFYPFFLDGIAAESRLNQRDGLHPTRAGVDAIVGRILPKVEELVARAREKRGS"
     gene            1677..>2120
                     /gene="thpR"
                     /locus_tag="VFU20_06425"
     CDS             1677..>2120
                     /gene="thpR"
                     /locus_tag="VFU20_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEU5286128.1"
                     /translation="MPRIFTGLEIPADVAQSLASLRGGLPGARWIDPENYHVTLRFIG
                     DVDESVAHEVASLLGRVRRGPFELYFDDLRSFGGRQPRALVATLGPVPALLELQAEHE
                     RLMQRIGLEPEGRKYTPHVTLARLRDTSNRQVAEYLALRAPYRSLP"
BASE COUNT          322 a          717 c          739 g          342 t
ORIGIN      
        1 ccaccacgca attcccgcaa cgcaaagcgc agcggcagcg aggcgggcga tttgtccgcc
       61 tcaggcggag ggagcctaaa cggcgcgttc atgagggctg cgcctcgcgg cggggctcgt
      121 gctcgatccg gccggagcgc agccgcacca cccggtcgca acgccgcgcg agcgcggtgt
      181 cgtgcgtgac caggaccagc gtggtgttgc gctcggcgtg cccggcgaac aacaggtcga
      241 tgatttcccg gcccgtggtc tcgtcgagat tgccggtggg ctcgtcggcg atgaggatcg
      301 ccggatccgg cgccagcgct cgcgccagcg cgacccgctg ctgttcgccg ccggaaagtt
      361 gcgccgggta atggtcgcgc cgttcggcga ggccgaccgc ggccaattcc cgctcggcac
      421 ggatgtgggc atcctggatg ccggcgagct cgagcggtac cgcgatgttc tcgagcgcgg
      481 tcatcgtcgg gatgaggtgg aaggactgga aaacgatacc aatgtggcgg ccgcggaagc
      541 gggccagcgc gtcctcgccc agccggccga gcgcctcgcc ggccacgacg acatcgccgg
      601 catcggcccg ttcgagcccg gccatgacca tcagcaacgt cgatttgccg gaaccggagg
      661 ggccgacgag gccaattgcc tcaccggcac ctatatgcag gtcgatgttc ttgaggatat
      721 ggacccgcgc cgcgccgcgt ccgagcgaca ggttgacccc ggtgagcgcg attgcgggac
      781 cgggcgccgg accgtggcct ggtccgccgc gaagagagga ttcctgcatg ccgtcgtccc
      841 gtcgctttct gttgccaccc cgcccggcgg tgcagggagt tgcgcctcgc tcatatggga
      901 gttttgccgc ctgggtccag acggtggccc gttacgccgg ggttccgctc ctggtcctgc
      961 tggtggcggt agcggcggcg ccggcggcgg agcggccggt gaagatcgtc gcgcttggcg
     1021 attcgctcac ggccggtctt ggactggcgg catccgaggc atttccggcc aagctcgaaa
     1081 aggcgctacg agcgcgcggc ctcgccgtgg agatcgccaa cgcgggggtg tcgggcgaca
     1141 ccgcttcggg aggtttggcc cggctcgact gggcagtgcc ggcggacacc gatgcggtca
     1201 tcgtcgaact cggcgccaac gacatgctgc ggggcacgga cccccagatt gcccgccggg
     1261 cgctggaaga aatcgtgcgg cggctgaccg accgtcggat tgccgtgctg ctcgccggca
     1321 tgcgtgccgc gcccaatctg ggacccgaat atgcgcggca attcgaggcg atctatccgg
     1381 aactggcggc gaagtacgat gtggtattct atccgttctt cctcgacggc atcgccgccg
     1441 agagccgcct caaccaacgc gacgggctcc acccgacccg ggctggtgtc gatgccatcg
     1501 ttggccgcat cctgcccaag gtcgaggaac tggtggcgcg cgcccgcgag aagcgcggtt
     1561 catgacggga actgtcacat ccatgtggga gcaacccgct agaaatgtcg cgcgaatcgc
     1621 cgccacatga tcaacttccg cctcaaggga acggttcgca ccctcgctga aatctcatgc
     1681 cccgcatttt caccggtctt gaaatccccg ccgacgtcgc tcagtcgctc gcatcgctgc
     1741 gcggcgggct tcccggcgcc cgctggatcg accccgagaa ctatcacgtg acactgcgct
     1801 tcatcggcga cgtcgacgaa tcggtcgcgc acgaagtggc gtcgctgctc ggacgggtgc
     1861 ggcgcgggcc gttcgagctc tatttcgacg acctcagatc gttcggcggc cgccagccgc
     1921 gtgccttggt tgcgacgttg gggccggtac cggcgctgct cgagctgcag gccgaacacg
     1981 agcgcctcat gcagcggatc gggcttgagc cggaagggcg caagtacacg ccccacgtta
     2041 cgctcgcccg cctgcgcgac acgtcgaacc ggcaggtcgc ggagtatctg gcgctgcgcg
     2101 cgccgtaccg ctcgctgccg
//
LOCUS       DASTZO010000230         2668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_851244,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000230 DASTZO010000000
VERSION     DASTZO010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2668
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851244"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VFU20_06430"
     CDS             complement(<1..513)
                     /locus_tag="VFU20_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286129.1"
                     /translation="MRRSIIALTAVALSFAAPAQTPTEGHYLFVWTADQAKEGNDFLV
                     VIDADPASQTYGKLVSGIGTEIKSVRIHHTEYDMPASHMLFANDHDSNQSVIFDLRDP
                     LKPKVAARFTSMSGYSMPHSFLRLPNGNVLASFQFADPGGHGDHATMSGKTGGLVEID
                     DRGKVVRAVSN"
     gene            complement(551..1231)
                     /locus_tag="VFU20_06435"
     CDS             complement(551..1231)
                     /locus_tag="VFU20_06435"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU5286130.1"
                     /translation="METIERPSPNFDERLLPVSMIVLHYTGMPDAEGALDRLTSPDSK
                     VSAHYLVDEDGSIYSLVDESMRAWHAGKSRWRGVSDVNSASVGIEIVNPGHEFGYRPF
                     PDEQIAALIPLVASIKERHAISRGNVVGHSDVAPARKEDPGELFPWEALARRRLALPS
                     PRRDLIDPFWTDAGFLLALERFGYDVTDPQKAVIAFQRRFRPDLIDGIIDGECRAKLL
                     ALLLPRPQ"
     gene            complement(1264..2139)
                     /locus_tag="VFU20_06440"
     CDS             complement(1264..2139)
                     /locus_tag="VFU20_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEU5286131.1"
                     /translation="MQVSDSSSRILASLLEARTGQQLTMSRRWRIETALAALLRERGI
                     ASLDELITILVMGREPSLSNQVVEALLNNETYFFRDRTPFDLLACHALPTLARSRESS
                     RRIRIWSAGCSTGQEAYSLAMLFAENQPAWRDWTIDILATDVSTSVIDRARSGIYTQF
                     EVQRGLGINQTIRWFEECDDGWRAVEPLRKPVRFQVHNLLEQPPHPGQFDIVLCRNVL
                     LYLCAEKRALAFERLASAMAPDGWLMLGAGETVIGQTRKLTSDSNARGLYRLVGEAGE
                     VEKCTGHDRRAVAGA"
     gene            complement(2130..>2668)
                     /locus_tag="VFU20_06445"
     CDS             complement(2130..>2668)
                     /locus_tag="VFU20_06445"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HEU5286132.1"
                     /translation="LGSLFQNLPPQIHVPILVTQHLPAPFMSVFARQLGVVAEREALV
                     AIDGMELVPDRMIIAPGDAHLTVDRVGGLDVVRLTTGQSSSGCMPSLDPMLASVGALF
                     GSASLGVVLTGMGRDGVEGAGRMVACGGSILAQDSASSAVWGMPRAVLEAGLACAVMP
                     PDQLARRIASRTGDSGCR"
BASE COUNT          429 a          864 c          939 g          436 t
ORIGIN      
        1 gttgctgacc gcacgaacta ccttgccacg atcgtcgatc tcgacgaggc cgcctgtctt
       61 gccgctcatc gtcgcatggt caccgtgacc gccgggatcg gcgaactgga aggaagcgag
      121 gacattgccg tttggcaggc gcaggaagct gtgcggcatg gagtagccgc tcatgcttgt
      181 aaaacgcgcc gcgaccttgg gcttcagcgg gtcgcgcagg tcgaagatta ccgattggtt
      241 agaatcgtgg tcgtttgcga aaagcatatg gctggcgggc atgtcatatt cggtgtggtg
      301 gatgcgcacg ctcttgattt cggtgccgat gcccgagacc aatttgccgt aggtctgtga
      361 tgcggggtcg gcgtcgatga ccacgagaaa atcgttgccc tccttggcct gatcggcggt
      421 ccagacgaaa aggtaatggc cctcggttgg cgtttgcgcc ggtgcagcga acgacagcgc
      481 gacggcggtg agcgcgataa tcgaacggcg catgatcttt ctcgcccggc agcgcgagat
      541 ggatgcgact ctattgcggt cttggcagga gcagcgccag cagtttcgcc cggcattcgc
      601 cgtcgatgat gccgtcgatg aggtcggggc ggaagcggcg ctggaaggcg atcacggcct
      661 tttgcgggtc ggtgacgtcg tagccgaagc gctcgagcgc gagcaggaag ccggcgtcgg
      721 tccagaacgg gtcgatgagg tcgcgccgcg ggctcggaag cgccagccgc cgccgggcca
      781 gcgcttccca cgggaacagc tcgcccgggt cctccttgcg cgccggggcg acgtcggaat
      841 ggccgacgac attgccgcgg ctgatcgcgt ggcgctcctt gatcgacgcc accagcggga
      901 tcagcgccgc gatctgctcg tcggggaacg gccggtagcc gaattcgtgc ccggggttga
      961 cgatctcgat tccgacgctg gccgaattga cgtcgctgac cccgcgccag cgcgactttc
     1021 cggcgtgcca ggcgcgcatg gattcatcga ccaggctgta gatgctgccg tcctcgtcga
     1081 ccagataatg ggcggacact ttggagtcgg gcgaggtcag tcggtcgagc gcgccctcgg
     1141 cgtcgggcat cccggtgtag tggaggacga tcatcgacac cggcagcagc cgctcgtcga
     1201 agttgggcga tggccgctcg atcgtctcca tgcgcgccat ggcgcacgag ccgatgcgat
     1261 cggtcaagcc ccggcgacgg ccctgcggtc gtggccggtg cacttttcga cctcgccggc
     1321 ctcgccgacc agccggtaga ggccgcgggc gttgctgtcg gacgtgagct tgcgggtctg
     1381 gccgatgacg gtctcgccgg cgccgagcat cagccagccg tccggcgcca tcgccgacgc
     1441 cagccgctca aacgccagcg cgcgcttttc ggcgcacagg tagagcagga cgttgcggca
     1501 cagcacgatg tcgaactggc ccgggtgcgg cggctgctcg agcaaattgt ggacctggaa
     1561 gcggaccggc ttgcgcagcg gctcgaccgc ccgccagccg tcgtcgcact cctcgaacca
     1621 gcggatggtc tggttgatcc cgagcccgcg ctggacctcg aactgggtgt agatgccgct
     1681 acgggcgcgg tcgatgaccg aggtcgagac gtcggtggcg aggatgtcga tggtccagtc
     1741 gcgccacgcc ggctggttct ccgcgaacag catcgccagg ctgtaggctt cctggccggt
     1801 cgagcagccg gcggaccaga tgcggatccg gcggctcgat tcgcggcttc gggcgagcgt
     1861 cggcagcgcg tggcaggcca gcaggtcgaa cggcgtccga tcgcggaaga aataggtctc
     1921 gttgttcagc aacgcctcca ccacctggtt ggacagactc ggctcgcgcc ccatgacgag
     1981 gatggtgatc agctcgtcga ggctggcgat gccgcgctcg cgcagcaagg cggcgagcgc
     2041 ggtctcgatc cgccagcgcc ggctcatggt cagttgctga ccggtccgcg cctcgagcag
     2101 gctggcgagg atccggctcg agctgtcgct cacctgcatc cggagtctcc ggtccgcgag
     2161 gcgatccggc gggcaagctg gtcgggcggc atcaccgcgc aggcgagccc cgcctcgagc
     2221 accgcgcgcg gcattcccca caccgcgctg ctcgccgaat cctgggcgag gatcgagccg
     2281 ccgcacgcga ccatccgccc ggctccctcg accccgtcgc ggcccatccc ggtcagcacc
     2341 acgccgagcg atgcggagcc gaacaacgcc ccgaccgagg cgagcatcgg gtcgagcgac
     2401 ggcatgcagc cgctcgacga ctggccggtc gtcagccgga cgacatcgag gccgccgact
     2461 cggtcgacgg tcaggtgcgc gtcgcccggg gcgatgatca tccggtcggg gaccagctcc
     2521 atgccgtcga tcgcgaccag cgcctccctc tccgcaacca ctccgagctg gcgcgcgaac
     2581 acgctcatga acggtgcggg aagatgttga gtgacgagga tcggcacatg aatctgtggg
     2641 ggcaaattct ggaaaaggct tccaagcg
//
LOCUS       DASTZO010000231         2217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_254769,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000231 DASTZO010000000
VERSION     DASTZO010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254769"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFU20_06450"
     CDS             <1..341
                     /locus_tag="VFU20_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5286133.1"
                     /translation="YADEIFWQQTKPELVTGSPSWKWVIEGFRSIREMRTSPRLKAMN
                     VPVLMLIAEADALVDPKAAASIASKLPDARVVRFGKESAHEILREGDKVRNRAIGEID
                     LFLAARAPKT"
     gene            338..1420
                     /locus_tag="VFU20_06455"
     CDS             338..1420
                     /locus_tag="VFU20_06455"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5286134.1"
                     /translation="MNRYDIAVVGAGIAGASLAAELAPHARVLVLEMEDQPGYHATGR
                     SAAFWSETYGGPYVQPLTTASGPLLKGYLEPLGSLHIARADEAAVVEAELASFAASGV
                     ALELVDPRERIPGLRDDWTMAVWEPSCTYIDVGGLHAKYLKAIRQAGGEIVTSAALEA
                     ADYDGGWKLRTRAGDFEADLLVNAAGAWADPVAVLAGAAPIGIQPYRRTMVQLATDPA
                     PPADLPHVGHITGSFYFKPEAGGRLWLSPHDEIACDPCDAQPEDIDVAIAIDRFEHAV
                     DWRVAKLEHKWAGLRSFAPDRLPVYGFDPKIERLFWCAGQGGFGIQTAPAAARLAAAL
                     VLDRSPDASVAHIDASRYSPGRFRAS"
     gene            1449..1619
                     /locus_tag="VFU20_06460"
     CDS             1449..1619
                     /locus_tag="VFU20_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1508 domain-containing protein"
                     /protein_id="HEU5286135.1"
                     /translation="MAHKFVIEQNKAGEYVAKFKYNSETIFWTEGYSSKAGAQNAIDS
                     IKKNGPGAYTDD"
     gene            complement(1642..>2217)
                     /locus_tag="VFU20_06465"
     CDS             complement(1642..>2217)
                     /locus_tag="VFU20_06465"
                     /inference="COORDINATES: protein motif:HMM:NF027479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4861 family protein"
                     /protein_id="HEU5286136.1"
                     /translation="LGAGGIGVWRGKVTQLGPSRISASIVNGRGQASVRVVSAGFAGK
                     GGPATLDATYSIAAGSRITRVDATITGAVPDMIAGLIRHPGVELVSGRTDHWAFAGTW
                     GNQSLAKDDLGMVLFYRHDRAEVQVGDTDISIHFCNPKALHYAFAAAWSQEAGGLRTR
                     QAFEAWAMQVIAQFERTDHEVKPAMTCSMDH"
BASE COUNT          354 a          732 c          766 g          365 t
ORIGIN      
        1 gctatgcgga cgaaatcttc tggcagcaga cgaagccgga actggtcacc ggctccccga
       61 gctggaaatg ggtaatcgag ggcttccgct cgatccgcga gatgcggacc tcgccgcggc
      121 tcaaggcgat gaacgtcccc gtgctgatgc tgatcgccga ggccgacgcg ctggtcgatc
      181 ccaaggcggc ggcgtcgatc gcatcgaagc tgcccgatgc ccgcgtcgtc cgcttcggca
      241 aggaaagcgc gcatgagatc ctgcgcgagg gcgacaaggt ccgcaaccgc gccatcggcg
      301 aaatcgacct gtttctcgcc gcgagggcgc cgaagacgtg aaccgttacg atattgcggt
      361 ggtaggggcg gggatcgcgg gggcgagcct ggccgccgaa ctggcgccgc atgcgcgggt
      421 gctggtgctc gagatggagg atcagccggg ttaccatgcg accggtcgct cggcggcgtt
      481 ctggtccgag acttatggtg ggccttacgt ccagccgctg accacggcgt cggggccgct
      541 gctgaagggg tatctggagc cgctgggctc gctccacatt gcccgcgccg acgaggcggc
      601 tgtggtggag gccgaactgg cgagcttcgc ggcgagcggg gtggcgctgg agctggtcga
      661 tccgcgcgag cgtatccccg gccttcgcga cgactggacc atggcggtct gggagccgag
      721 ctgcacgtac atcgacgtgg gcgggctgca cgcgaagtat ctcaaggcga tccggcaggc
      781 ggggggcgag atcgtcacct cggcggcgct ggaagcggcg gactatgacg gcgggtggaa
      841 gctgcgcacg cgcgcgggcg atttcgaagc ggacctgctg gtcaatgcgg cgggcgcctg
      901 ggccgatccg gtcgcggtgc tggctggcgc ggcccccatc ggcatccagc cctatcgccg
      961 caccatggtc cagctcgcca ccgatcccgc cccgcccgcg gacttgccgc atgtcggcca
     1021 catcaccggc agcttctatt tcaagcccga ggccggcggg cggctatggc tcagcccgca
     1081 tgacgagatc gcgtgcgatc cctgcgacgc ccagcccgag gatattgacg tcgccatcgc
     1141 catcgaccgg ttcgaacatg ccgtcgattg gcgggtcgcc aagctcgagc ataaatgggc
     1201 cggcctgcgc agcttcgccc ccgatcgcct gcccgtctat ggcttcgatc cgaagatcga
     1261 gcgcctgttc tggtgcgcgg ggcagggcgg cttcggcatc cagaccgccc ccgccgcggc
     1321 caggctggcg gcggcgctgg tgctggaccg gtcgcccgac gcatcggtcg cgcatatcga
     1381 cgcttcccgc tattccccgg gccggtttcg cgctagctga tctgcatcag ttcgcaggga
     1441 gtaggaccat ggcccacaag tttgtgatcg agcagaacaa ggccggcgaa tatgtcgcca
     1501 agttcaaata taacagcgaa acgatcttct ggaccgaggg ctatagctcg aaggccggtg
     1561 cccagaacgc gatcgattcg atcaagaaga acgggccggg cgcctacacc gacgactgag
     1621 gcgggcgccg gcggccggga cctaatggtc catgctgcag gtcattgccg gcttcacctc
     1681 atggtctgtc cgttcgaatt gggcgatgac ctgcatcgcc caggcttcga atgcctggcg
     1741 ggtcctgagt ccgcctgcct cctggctcca cgcggcggcg aaggcatagt gcagcgcctt
     1801 ggggttgcag aaatggatgc tgatgtcggt gtcgcccacc tgtacctcgg cgcggtcgtg
     1861 ccggtagaac agcaccatgc cgagatcgtc tttggccagg ctctgattgc cccaggtgcc
     1921 ggcaaaggcc cagtgatcgg tcctgcctga cacgagttcg acgccgggat ggcgaattag
     1981 ccccgcgatc atatccggca ccgcgccggt gatggtcgca tcgacccggg tgatgcgcga
     2041 tcccgccgcg atcgaatagg tcgcgtcgag cgttgccggg ccgcccttcc cggcgaaccc
     2101 ggcgctgacg acgcgcacac tcgcctggcc gcgcccgttg acgatgctgg cgctgatccg
     2161 cgagggacca agctgcgtca ccttgccccg ccacaccccg atcccgcccg cgcccag
//
LOCUS       DASTZO010000232         5339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1142686,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000232 DASTZO010000000
VERSION     DASTZO010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1142686"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1294
                     /locus_tag="VFU20_06470"
     CDS             29..1294
                     /locus_tag="VFU20_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030253 - protein secretion by the type I
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HEU5286137.1"
                     /translation="MGPIKRQRQPLTGSRLIIVASAIGFAIFLIWAMLAQVDEVTSGQ
                     GKVIPSSKAQLIQAAEPAIVQELLVRSGQRVRRGQLLARLDDTQSASELGQIQAETEA
                     LTARSARLAAEGTRTSTNCVGADCADEAQLRQVRESALRSRISALQASADQRRREASE
                     AQATITSLQGSLALAQRQVAMLEPLAAKNIVPQTDLLDARREVNDLQGRIAAAREQQS
                     RALAAVREAQAQASEAGFSFRQEALNERSQIEAKLAVNKESLRGARGRLGRMELRSPV
                     EGVVNDLQVTTIGGFVQAGQKVMEIIPMGDKLLVETRVKPSDIAFIKVGDRALVKVTA
                     YDFSTYGGLDGRVVQVSADSIYDEVDKEAYFNVIVETDKSYLQSAGRMLPITPGMMTD
                     TQIITGRKSILSYLLKPVLRARSEALRER"
     gene            complement(1291..2181)
                     /locus_tag="VFU20_06475"
     CDS             complement(1291..2181)
                     /locus_tag="VFU20_06475"
                     /inference="COORDINATES: protein motif:HMM:NF017815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HEU5286138.1"
                     /translation="MEKTVTSRLVGRLAATLAVALAPAPALAQSAVAQPASWGVSYSK
                     SEAILGAPSRLAAILAEQQGLPAPKPLRPAFYSARTPGYAVLRETSRFSPGAVSGRPD
                     VFGSVALAVGRTSLDRRWRAVARKRVGGAAAAYAAGLRGRSDIARVEAVNRYVNHRVQ
                     FVDDSRQYGRDDLWSAASETLRRGRGDCEDYAIAKLQMLRAAGFSDRDLYLVVAKDLV
                     RRADHALLVVRAGGRMLVLDNGTDAILNADNPGDYRPVLTFAANGAWTHGYRRRMPSI
                     AVAAATVAPLTPSGPATLAD"
     gene            complement(2297..3469)
                     /gene="wecB"
                     /locus_tag="VFU20_06480"
     CDS             complement(2297..3469)
                     /gene="wecB"
                     /locus_tag="VFU20_06480"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HEU5286139.1"
                     /translation="MQIAPAEVGGSLGSADIRLVIGTRPEAIKLAPVARALAARDVRP
                     SLILTGQHADLDPADHGLGAYSATRLHCPGAQDPHSHVRSVTAAVLEQLRKPPDLLVV
                     QGDTSSALGGALAGFSAGVPVAHVEAGLRSHDSAMPWPEEEYRTAIDANADLLFAPTA
                     LAAANLRREGVGGTIFVTGNTGIDAVMKVRSELPPPSLRDRALPRLLVTCHRRENWGD
                     GLGSVAMALRQLADEGTASIDVVLHPNPHVTRTIERLLGGCFAITLLPPCSQRRLVAR
                     MRDCDLMLSDSGGVQEEAPALGVPLLVLREKTERPEALWSGNMRLVGTDTDQILAAVR
                     DLLGDPVALAAMAEPSLPYGDGRAGYRIAALIEHWLAARSVESSARRPSAQELRRS"
     gene            complement(3508..3789)
                     /locus_tag="VFU20_06485"
     CDS             complement(3508..3789)
                     /locus_tag="VFU20_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HEU5286140.1"
                     /translation="MTVARISELDRRTTQSGKANTGRWLLEFERQDPLRADPLTGWAG
                     SRDTRPQVRLNFDSKEAAIAYATRHGLAYHLVPAKPVKLKIQAYADNFR"
     gene            3927..4502
                     /locus_tag="VFU20_06490"
     CDS             3927..4502
                     /locus_tag="VFU20_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286141.1"
                     /translation="MLLISAAIFACAGCDSQPAAVDTADSNEAAAEAATDHGDPIKSA
                     MAAAPESISGNAAIVQAGADGSMKTLREGTNGWTCMPDNPSTPGHDPMCMDANAGQWA
                     MAWIGKKTPPANTVGVMYMLAGGTDASNTDPYAQKPTEENAWIETGPHLMVVGSQEVL
                     KGHPSGPKPDTKAPYVMWAGTPYAHLMIPVG"
     gene            complement(4518..>5339)
                     /locus_tag="VFU20_06495"
     CDS             complement(4518..>5339)
                     /locus_tag="VFU20_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5286142.1"
                     /translation="TLACANLLGYPGTRHHKLAAAVEFIHTATLLHDDVVDGSGMRRG
                     KRTANIIWGNPASVLVGDFLFSRAFELMVEDGSLKVLRILSHASAVIAEGEVEQLTAQ
                     RRIDTGEEQYLEIIGAKTAALFAAACRISPVVAEAGEEAETALESYGRNLGIAFQLVD
                     DVIDYASDATTMGKGVGDDFRDGKMTLPLILAYARGSAAERSFWEAAVAGERISDEDL
                     AQAIALMRSSEALADTIDRARHYARRAIDALGPFPPGKAKAALTEAVEFAVARAY"
BASE COUNT          857 a         1801 c         1928 g          753 t
ORIGIN      
        1 aagcgtagcc atggccaccc ttcccttcat gggtccgatc aagcgccagc gccagccgct
       61 gaccggctct cggctgatca tcgtcgcctc ggcgatcggc tttgccatct tcctcatttg
      121 ggcgatgctc gcgcaggtcg acgaagtcac cagcggccag ggcaaggtga tcccgtcgag
      181 caaggcgcag ctgatccagg cggccgagcc ggcgatcgtc caggaattgc tggtgcgttc
      241 gggccagcgg gttcgccgcg gccagctgct cgcccgcctc gacgacaccc agagcgcctc
      301 cgagctcggc cagatccagg ccgagacgga agccctgacg gcgcgctcgg cgcgtctcgc
      361 cgcggagggg acgaggacct cgaccaactg cgtcggggcg gattgcgccg atgaagcgca
      421 attgcggcag gtgcgcgagt cggcgcttcg gagccgcatc tcggcgctcc aggcgagcgc
      481 cgaccagcgc cgccgcgaag cgtccgaagc gcaggcgacc atcaccagcc tgcagggcag
      541 cctcgcgctg gcccagcggc aggtggcgat gctcgagccg ctggcggcca agaatatcgt
      601 cccgcagacc gacctgctcg acgcccggcg cgaagtgaac gacctgcagg ggcggatcgc
      661 ggcggcccgc gagcagcaat cgcgcgcctt ggccgcggtc cgcgaagcgc aggctcaggc
      721 cagcgaggcc ggcttcagct tccgccagga agcgctcaac gagcgcagcc agatcgaggc
      781 caagctcgcg gtcaacaagg aatcgctgcg cggggcccgc ggccggctcg gccggatgga
      841 gcttcgctcg ccggtggagg gggtcgtcaa cgacctccag gtgacgacca tcggcggctt
      901 cgtccaggcc ggccagaagg tcatggaaat catcccgatg ggcgacaaat tgctggtcga
      961 gacgcgcgtc aagccgagcg acatcgcctt catcaaggtc ggcgaccggg cgctggtcaa
     1021 ggtcaccgcc tacgacttct cgacctacgg cgggctcgac ggacgggtcg tccaggtgtc
     1081 ggcggactcg atctacgacg aggtcgacaa ggaggcctat ttcaacgtca tcgtcgaaac
     1141 cgacaaaagc tatctccagt cggccgggcg gatgctcccg atcacgccgg gaatgatgac
     1201 cgacacgcag atcatcaccg ggcgcaagag catcctgagc tatctgctaa agccggtcct
     1261 gagagcccgc agcgaggcgc tgcgcgagcg ctagtcggcg agggtcgccg gaccggaagg
     1321 agtcagcggg gcgaccgtcg ccgccgccac cgcgatcgac ggcatccggc ggcggtagcc
     1381 gtgggtccag gcgccattgg cggcgaacgt cagcaccggc cggtagtcgc cggggttgtc
     1441 ggcgttgagg atcgcgtcgg tgccgttgtc gagcaccagc atgcgccccc cggcgcggac
     1501 caccagaagc gcgtgatcgg cccggcggac gagatccttg gcgacgacca ggtacaggtc
     1561 gcggtcggag aaaccggcgg cgcgaagcat ctgcagcttg gcgatcgcat aatcctcgca
     1621 gtcgccgcgg ccgcggcgga gcgtctcgct ggccgccgac cacaagtcgt cgcggccgta
     1681 ctggcgcgag tcatcgacga actggactcg gtggttgacg tagcggttga cggcctcgac
     1741 ccgggcgatg tccgaccggc cgcggagccc ggccgcatag gcggcggccg cgccgccgac
     1801 ccgtttgcgg gcgaccgcac gccagcgccg gtcgagcgaa gtgcggccga ccgcgagcgc
     1861 gacgctgccg aacacgtcgg ggcggccgct gaccgcgccg gggctgaagc ggctcgtttc
     1921 gcgaagcacg gcgtagccgg gagtccgggc gctgtagaat gcgggccgaa gcggcttggg
     1981 ggcgggaagc ccctgctgct cggcgagaat ggccgccagt cggctgggcg cgccgaggat
     2041 cgcttccgac ttggagtagc tgacgcccca gctcgcgggc tgtgcgacgg cgctctgggc
     2101 cagcgcgggc gccggcgcca gggcgacggc aagcgtggcg gcaaggcgcc cgacgagccg
     2161 gctcgtgacg gtcttttcca tgccccggct tgtcgcagga caaggacaag acacggctaa
     2221 agaccatggt taatgcggcg ctaaccattg gggaaaccta tccattttgg gtaatcgggc
     2281 gggcgtcccg gggggcctag ctgcggcgga gctcctgggc gctcgggcgc cgcgccgagg
     2341 actcgaccga gcgggcggcg agccagtgct cgatcagcgc ggcaatccgg tagccggccc
     2401 gcccgtcgcc ataaggcagg ctcggctcgg ccatcgccgc gagggcgacc gggtcgccga
     2461 gcaagtcgcg gaccgccgcg aggatctggt cggtatcggt tccgaccagc cgcatgttgc
     2521 cgctccacag cgcttccggc cgctcggtct tctcgcgcag caccagcagc gggaccccga
     2581 gcgcgggcgc ttcctcctgg actccgcccg aatcgctcag catcaggtcg cagtcacgca
     2641 tcctggcgac gagccggcgc tggctgcacg gcggcagcag ggtaatcgcg aagcagcctc
     2701 cgagcagccg ctcgatggtc cgggtgacgt gcggattggg gtgcagcacg acgtcgatgc
     2761 tggcggtgcc ctcgtcggcg agctggcgaa gcgccatcgc caccgagccg aggccgtcgc
     2821 cccaattctc gcggcggtga caggtgacca gcagccgggg cagggcgcgg tctcggaggc
     2881 tgggcggcgg aagctcggag cggaccttca tcaccgcgtc gatgccggtg ttgccggtga
     2941 cgaagatcgt gccgccgacg ccctcgcggc gaaggttggc ggcggcgagc gccgtcggcg
     3001 cgaacagcaa atcggcgttg gcgtcgatcg ccgtccgata ctcctcctcg ggccagggca
     3061 tcgccgagtc atggctgcgc agccccgcct cgacgtgcgc gaccggcacc ccggcgctga
     3121 atcccgccag cgcgccgccg agcgcgctcg acgtgtcgcc ctggacgacc agcaggtcgg
     3181 ggggcttgcg cagctgctcg aggaccgccg cggtgacgct gcggacgtgc gagtgcgggt
     3241 cctgggcgcc ggggcaatgg agccgggtcg ccgaataggc gccgagtccg tgatcggcgg
     3301 ggtcgagatc ggcatgctgg ccggtgagga tcaggctcgg ccggacgtcg cgagcggcga
     3361 gcgctcgggc gacgggggcc agcttgatgg cttcggggcg ggtgccgatg acaagcctga
     3421 tatccgcgga acccagactt ccccctacct cggctggggc aatctgcatc cgcaatggac
     3481 aaagtcaagc tatgttaaca acataggcta gcggaaattg tcggcgtagg cctggatctt
     3541 gagcttcacc ggcttggccg ggacgagatg gtaagcaagg ccgtggcggg tcgcataagc
     3601 gatcgcggct tccttgctgt cgaaattcag ccgcacctgc ggccgcgtgt ctcgcgatcc
     3661 ggcccagccg gtcagcgggt cggcacggag cgggtcctgg cgctcgaact cgagcagcca
     3721 gcggccggtg ttggccttgc ccgactgggt ggtccggcga tcgagctcgg aaatgcgcgc
     3781 gacggtcatc gcctcgcgag gtggcaaagg cgcagcccgg aatcaagtgc gcgtcgccgc
     3841 ggccgctgcc caatgccggc gaagctgcta atggccgggc tccaggaaaa aagaggagga
     3901 gccttatgtt gccgtcaaaa gcgtctatgc ttctgatctc cgccgcgatt ttcgcgtgcg
     3961 cgggctgcga ctcgcaaccg gccgcggtcg acacggccga ctccaacgaa gccgccgccg
     4021 aagccgccac cgaccacggc gacccgatca agagcgccat ggccgctgcc cccgaatcca
     4081 tctccggcaa tgcggccatc gtccaggcgg gcgccgacgg gtcgatgaag acgctgcgcg
     4141 agggcaccaa cggctggacc tgcatgcccg acaacccgtc gaccccgggc cacgacccga
     4201 tgtgcatgga cgccaatgcc ggccagtggg caatggcgtg gatcggcaag aaaacgccgc
     4261 cggcgaacac cgtcggagtg atgtacatgc tggccggcgg gacggacgcc agcaacaccg
     4321 atccctatgc ccagaaaccg accgaggaga acgcctggat cgagaccggg ccgcacctga
     4381 tggtggtcgg atcgcaggag gtgctcaagg gccatccgtc ggggcccaag ccggacacca
     4441 aggcgccgta cgtgatgtgg gccggaacgc cctacgcgca tctgatgatc ccggtcggct
     4501 aggccggtcc gcgcccttca gtaggcgcgg gcgaccgcga actcgacggc ttcggtcagc
     4561 gccgccttgg ctttccccgg cgggaacggg ccgagcgcgt cgatcgcgcg ccgcgcgtag
     4621 tgccgcgccc ggtcgatggt gtccgccaag gcctcgctcg accgcatcag cgcgatcgcc
     4681 tgggcgaggt cctcgtcgga gatccgctcg ccggcgaccg cggcctccca gaaggagcgc
     4741 tcggcggccg agccgcgggc gtaggcgagg atcagcggca gggtcatctt gccgtcgcgg
     4801 aaatcgtcgc cgacgccctt gcccatggtc gtcgcgtccg acgcgtaatc gatcacgtcg
     4861 tcgaccagct ggaaggcgat gccgaggttg cgcccgtagc tctcgagcgc ggtttcggcc
     4921 tcttcgccgg cttcggcgac caccggtgag atccggcatg cggcggcgaa cagggcggcg
     4981 gtcttggcgc cgatgatctc gagatattgc tcctcgccgg tgtcgatccg gcgctgcgcg
     5041 gtcagctgct cgacctcgcc ctcggcgatc accgcggagg catggctgag gatgcggagc
     5101 accttcaggc tgccgtcctc gaccatcagc tcgaatgctc ggctgaacag gaaatcgccg
     5161 actagcacgc tcgccgggtt gccccagatg atgttcgcgg tgcgtttccc gcggcgcatc
     5221 ccggagccgt cgacgacatc gtcatggagc agggtcgccg tgtggatgaa ctccaccgcg
     5281 gccgcgagct tgtggtggcg ggtccccggg tagccgagca ggttcgcgca ggcgagcgt
//
LOCUS       DASTZO010000233         7283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_176375,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000233 DASTZO010000000
VERSION     DASTZO010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7283
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176375"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1410
                     /gene="ftsH"
                     /locus_tag="VFU20_06500"
     CDS             <1..1410
                     /gene="ftsH"
                     /locus_tag="VFU20_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU5286143.1"
                     /translation="VEVVEFLKFPDRFAALGARIPRGVLLVGPPGTGKTLIARAVAGE
                     ANVPFFTISGSDFVEMFVGVGASRVRDMFDQAKKSAPCIVFIDEIDAVGRHRGAGLGN
                     GNDEREQTLNQLLVEMDGFEANEGIIIIAATNRPDVLDPALLRPGRFDRQVVVPRPDI
                     DGREQILHVHMKKVPLAPDVDPRVIARGTPGFSGADLANLVNEAALLAARRGKRLVAM
                     KEFEEAKDKVLMGTERKSMVMTEDEKKMTAYHEAGHAIVALHEPASDPIHKATIIPRG
                     RALGMVMRLPERDNYSYHRDKMYANLAVSMGGRVAEEIIFGYDKVSSGASSDISYATS
                     LARDMVTRWGMSDALGPLQYADADEEVFLGYSVNRQRQMSNETAQLIDKEIRRVVEEG
                     YERAKKLLSENQEELHTLAKALLEYETLSGDEIRVLLDGGTIDRGGASTPGIPAAGSS
                     IPKARRKTRGIGGPAAAGA"
     gene            complement(1412..2461)
                     /locus_tag="VFU20_06505"
     CDS             complement(1412..2461)
                     /locus_tag="VFU20_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3667 domain-containing protein"
                     /protein_id="HEU5286144.1"
                     /translation="MPDLESIGDAATGAVIARAVEPRAGEADDEHTHETRCLNCGALL
                     VGDYCHECGQRAHVHRSLKGFFDDLLHGVLHFEGKIWRTLPKLVGNPGDLTRRYIDGE
                     RSRFISPIALFLFSVFLMFAILSLAGPTLSPSGAEMGAEIAGEIEAEKARLARLEAER
                     SSAIAAGAPTGALDSRIREKRAEVEMLELMRGRGSGDRNPPREADTAGNSWIETAYLK
                     ARQNPDLLIYKLKTNAYKFSWALIPLSVPFLWLLFPFSRRFRLYDHTVFVTYSLSFMT
                     LLIIGALLLGTAGLGSIASLAFVVPPIHMYLQLKDTYGLTRTAALWRTALLVTFAFVA
                     VTVYALLLLALGVLD"
     gene            2524..3183
                     /locus_tag="VFU20_06510"
     CDS             2524..3183
                     /locus_tag="VFU20_06510"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU5286145.1"
                     /translation="MKRIGLLGGSFNPAHRGHRRISLAAIDALGLDEVWWLVSPGNPL
                     KAGREMAPFEARLESARAMARRAPIRVSDFEARAGTRFSIDTLGELLRAHPRDRFIWL
                     MGADTIAQFHQWKDWRALARTVPIAVISRPGYEGAARAARAMGWLRRFVRPSGQARNW
                     TDWSAPAILLLRLPPDPTSATALRALDPNWHRRPQSRARPQPGGAPSSVRRALARSSP
                     R"
     gene            3221..3589
                     /gene="rsfS"
                     /locus_tag="VFU20_06515"
     CDS             3221..3589
                     /gene="rsfS"
                     /locus_tag="VFU20_06515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU5286146.1"
                     /translation="MDVEDLHRLVLKSLDDDQAVEVISIPLAGKSSIADHMVIASGRS
                     TRQVASMASKLAQKIKDQVGRSPRIEGLPAADWVLIDADDVIVHLFRPEVRTFYNLER
                     MWAFGDDASPQRSAGAAPGS"
     gene            3595..4014
                     /locus_tag="VFU20_06520"
     CDS             3595..4014
                     /locus_tag="VFU20_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006962282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU5286147.1"
                     /translation="MLLHIVARGKIGRSPEAELVERYLKRIAWPTRVTELSERAELPA
                     LPATSVSIALDERGTALSSAELARKLEAWRDGGKREARFLIGAAGGHDDAQRDRADLL
                     LSFGPATWPHLLVRAMLAEQLFRATTILANHPYHREG"
     gene            4020..5201
                     /locus_tag="VFU20_06525"
     CDS             4020..5201
                     /locus_tag="VFU20_06525"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU5286148.1"
                     /translation="MRRFAALLLAVPLLLAASAPVQPVSETVDEALMRARAEARAADA
                     KVRKLEQAAGQARGEAARLHARQAAAAEAIAAAEARISAADAERRIIAARIAARRQRL
                     ERQQRPIASLLAGLAMMARRPPLLALTSDSSMEEFVRVRLLLDESLPVIRRRTAALSA
                     ELARGQRLERAALASLQSLARSRDELSERRREFAALEARALRSAELSGSAALGAGDVA
                     LAMGEDIERLAEETRRNRSAAQIAAELAALGPAPPRPAPAEGRPAQPPLDYRLPSAAR
                     VVEGLGEVNSSGVRSRGLTLATRRGTEVVVPAAGIVRFAGPFRDYDGIAIIDHGGGWM
                     SLIVNVASPLRPGAKVAAGERLGRALGPLGVELSHQGRRASPALIAGSSATLSNRPKG
                     G"
     gene            5237..6577
                     /locus_tag="VFU20_06530"
     CDS             5237..6577
                     /locus_tag="VFU20_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5286149.1"
                     /translation="MTRKLLPPLALVGALALVPVTTSSLAAADVANYEELETFMSVYE
                     RVKANYVDPVDDHTLIKGAIDGMLAALDPHSSYVEASDFQTLRTTTDGNYGGLGLTVS
                     TEDGAVKVISPTEDTPAWRAGIKAGDFITHINSELLYGLTLDEAVDKLRGPPGSAIKV
                     TIVRPGRDKPFDVTMVRERIVLRPVKWEIKDGVGVININSFAANTGDATRAALVAIDK
                     AAGGRPIGYVVDLRSNPGGLLDQAIEVSDTFLNHGEIVSQRGREKDDIERYYAKPGDL
                     AHGLPVVILVDAGSASAAEIVAGALQDHRRAIVMGERSFGKGSVQTVVQTGPDSALRL
                     TTARYYTPSGKSVQAGGIDPDIVVPQLTDPDYKDRPRVREADLRRHLISQAKADDKLL
                     EHDETTDPRFAATAAELEKKGVKDFQLEYAIRTLKRLAPGTAAIASAGGRPKTR"
     gene            6588..7112
                     /locus_tag="VFU20_06535"
     CDS             6588..7112
                     /locus_tag="VFU20_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HEU5286150.1"
                     /translation="MSEATLGRRPAPLAQSGPALARWIALLLPLALLGGALGSQYLGG
                     LAPCEMCYWQRWPHGAAIALAGLAFAGPPASGRSRTLTLLAALAIAVSGAIGVYHAGV
                     ELGVFEGLTTCAAGVSGATNAELLDQIMKTPLVRCDQVQFAFLGISMAGWNAILSLGG
                     AALIAWLTLGRRAA"
     gene            7154..>7283
                     /locus_tag="VFU20_06540"
     CDS             7154..>7283
                     /locus_tag="VFU20_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HEU5286151.1"
                     /translation="MLRVNQAGEYGATRIYAGQLAVLRGNSPASKLIARMAGQERRH"
BASE COUNT         1122 a         2591 c         2532 g         1038 t
ORIGIN      
        1 gtcgaggtcg tggaattcct caagttcccc gatcgcttcg cggcattggg cgcgcggatt
       61 ccgcggggcg tgctgctggt cggcccgccg ggcaccggca agacgctgat cgcgcgcgcg
      121 gtcgccggcg aggccaacgt gccgttcttc accatctccg gttcggactt cgtcgagatg
      181 ttcgtcggcg tcggcgccag ccgcgtgcgc gacatgttcg accaggccaa gaaatcggcg
      241 ccgtgcatcg tcttcatcga cgagatcgac gcggtcggcc gccatcgcgg cgccgggctc
      301 ggcaacggca acgacgagcg cgagcagacc ttgaaccagc tgctggtcga gatggacggc
      361 ttcgaggcga acgagggcat catcatcatc gccgcgacca accgccccga cgtgctcgac
      421 ccggcgcttc tgcggcccgg ccgcttcgac cgccaggtcg tcgtcccgcg cccggacatc
      481 gacggccgcg agcagatcct ccacgtccac atgaagaagg tgccgctggc gcccgacgtc
      541 gatccgcggg tgatcgcccg cggcaccccc ggcttctcgg gcgccgacct cgccaacctc
      601 gtcaacgagg cggcgctgct cgccgctcgc cgcggcaagc ggctggtcgc catgaaggag
      661 ttcgaggagg ccaaggacaa ggtgctgatg ggcaccgagc gcaagtccat ggtgatgacc
      721 gaggacgaga agaagatgac cgcctatcac gaggccggtc acgcgatcgt cgccctccat
      781 gagccggctt cggacccgat ccacaaggcg acgatcatcc cgcgcggccg cgcgctggga
      841 atggtcatgc gcctgcccga gcgcgacaat tacagctatc accgcgacaa gatgtacgcg
      901 aacctcgccg tctcgatggg cggccgcgtc gccgaggaga tcatcttcgg ctacgacaag
      961 gtgtcgtccg gcgcctcgtc cgacatctcc tacgcgacca gcctggcgcg cgacatggtc
     1021 acccgctggg gcatgtccga cgccctgggc ccgctgcaat atgccgacgc cgacgaggag
     1081 gtgttcctcg gctattcggt caaccgccag cggcagatgt cgaacgagac cgcgcagctg
     1141 atcgacaagg agatccgccg cgtcgtcgag gaaggctatg agcgcgccaa gaagctgctg
     1201 agcgagaacc aggaagagct gcacacgctc gccaaggcgc tgctcgaata tgaaaccctg
     1261 agcggcgacg agatcagggt cctgctcgac ggcggcacga tcgaccgcgg cggcgcctcg
     1321 accccgggca tcccggccgc gggctcgtcg atcccgaagg cgcgtcgcaa gacccggggg
     1381 atcggcggac cggccgccgc cggcgcctga tctagtcgag gactccgagc gccagcagca
     1441 gcaaggcgta gaccgtcacc gcgacgaagg cgaaggtcac cagaagcgcg gtgcgccaga
     1501 gcgccgcggt gcgggtcagg ccgtaggtgt ccttgagctg caggtacatg tggatcggcg
     1561 gcaccacgaa cgccagcgag gcgatgctcc cgagcccggc cgtccccagc agcagcgcgc
     1621 cgatgatcag cagggtcatg aagctcagcg aataagtgac gaacaccgtg tggtcgtaga
     1681 gccggaagcg gcggctgaac gggaacagca gccacaggaa cggcaccgac agggggatca
     1741 gcgcccagct gaacttgtag gcgttggtct tgagcttgta gatcaacagg tcggggttct
     1801 gcctggcctt gagataggcg gtctcgatcc aggaattgcc ggcagtgtcg gcctcgcgag
     1861 gtgggttgcg gtccccgctc ccgcggccgc gcatgagctc gagcatctcg acctcggcgc
     1921 gcttttcccg gatccggctg tcgagcgcgc cggtcggagc accggccgca atcgcgctgg
     1981 accgctccgc ctcgagccga gccagcctgg ccttctccgc ctcgatctcg ccggcgattt
     2041 cggcgcccat ttcagccccg gacgggctca gcgtcgggcc ggcgaggctg aggatcgcga
     2101 acatcaggaa gaccgagaac aggaacaagg cgatcggcga gataaaccgc gaccgctcgc
     2161 cgtcgatgta gcggcgggtg aggtcgcccg ggttgccgac gagcttcggc agggtgcgcc
     2221 agatcttgcc ctcgaaatgc agcacgccgt gcaggaggtc gtcgaagaag cccttgaggc
     2281 ttcggtggac gtgggcccgc tggccgcatt cgtggcaata gtcgccgacc agcagggcgc
     2341 cgcaattgag gcagcgggtt tcgtgggtgt gctcgtcgtc ggcctctccg gcgcgcggct
     2401 cgacggcgcg tgcgatcacc gcgccggtcg ctgcgtcgcc gatcgattcc aggtcgggca
     2461 tcgccactcc ttcgggcccg gacattaggc gctgcctggc ggccatgcta gctgctcggg
     2521 ccgatgaagc gcatcggact tctcggcgga tcgttcaatc cggcgcaccg cgggcaccgc
     2581 cggatcagcc tcgcggcgat cgacgcgctc ggcctcgacg aggtctggtg gctggtgtcg
     2641 cccggcaacc cgctcaaggc cggccgggag atggcgccgt tcgaggcgcg gctggaatcg
     2701 gcgcgggcga tggcgcgccg ggcgccgatc cgggtcagcg atttcgaggc tcgggcgggc
     2761 acccggttca gcatcgacac cctcggcgag ctgctgcgag cccatccgcg cgaccgtttc
     2821 atctggctga tgggcgccga cactattgcc caattccatc aatggaagga ctggcgcgcg
     2881 ctggccagga ccgtgccgat tgcggtgatc tcgcgccccg gctatgaagg agctgcccgc
     2941 gcggcgcgcg cgatgggctg gcttcggcgg ttcgtccgac cttcgggcca ggcacgcaac
     3001 tggacggatt ggagtgcacc ggcgattttg ctgcttcgcc tgccgcccga tccgacgtcc
     3061 gccaccgcct tacgcgcgct cgaccccaac tggcaccgcc gcccgcagtc ccgagcccgg
     3121 ccgcagcctg gcggcgcgcc atcttccgtc aggagagcac ttgcccgatc gtccccccgt
     3181 tagcccgagc ggcgcgaccg cccgcaacgg ccgtccgcca atcgacgtcg aagacctcca
     3241 ccgtctcgtc ctcaaatcgc tcgacgacga ccaggccgtc gaggtcatct cgatcccgct
     3301 ggcgggcaag tcgagcatcg ccgaccatat ggtgatcgcc tcgggccgct cgacccggca
     3361 ggtcgcgtcg atggccagca agctcgctca gaagatcaag gaccaggtcg gccgctcgcc
     3421 gcggatcgag ggccttccgg ccgccgactg ggtgctgatc gacgccgacg acgtgatcgt
     3481 gcacctgttc cggcccgagg tccgcacctt ctacaatctc gagcggatgt gggcgttcgg
     3541 cgacgacgcc tcgccgcagc gcagcgccgg cgcggcgccc ggctcctaac ggagttgctg
     3601 ctccacatcg tcgcccgcgg caagatcggc cgctctcccg aagccgagct cgtcgagcgc
     3661 tacctgaagc gcatcgcctg gccgacccgg gtcaccgaac ttagcgagcg cgccgagctt
     3721 cccgccctgc cggccaccag cgtctcgatc gccctcgacg agcgcggcac ggccttgtcc
     3781 tcggccgagc tggcgcgcaa gctcgaagcc tggcgcgacg gcggcaagcg cgaagcccgg
     3841 ttcctgatcg gcgccgccgg cggccatgac gatgcgcagc gcgacagggc cgatctgctg
     3901 ctgtcgttcg ggccggcgac ctggccgcac ctgctggtcc gggcgatgct ggccgagcaa
     3961 ttgttccggg cgacgacgat ccttgcgaac cacccctatc atcgcgaagg atgagcgcga
     4021 tgcgccgctt cgccgctttg ctgctcgccg ttccgctgct gctcgcggcg agcgcgccgg
     4081 tgcaacccgt gtccgagacc gtcgacgagg ccttgatgcg cgcacgggcc gaagcgcgcg
     4141 cggccgacgc gaaggtccgg aagctggagc aggcggccgg ccaggcgcgc ggcgaggcgg
     4201 cccggctgca cgcccggcag gccgccgccg ccgaggcgat cgccgccgcc gaggcgcgga
     4261 tcagcgccgc cgacgccgag cggcggatca tcgccgcgag gatcgccgcg cgccgccagc
     4321 ggctcgagcg gcagcagcgg ccgatcgcct cgctgctcgc gggactggcg atgatggccc
     4381 ggcgcccgcc cttgctcgcc ttgaccagcg attcgagcat ggaggaattc gtccgggtgc
     4441 ggctgttgct cgacgagagc ctgccggtca tccggcggcg cacggcggcg ctgtcggccg
     4501 agctggcccg cgggcagcgg ctcgagcgcg ccgcgctcgc ctcgctccag tcgctggctc
     4561 ggagccgcga cgagctgtcg gaacggcgcc gcgagttcgc cgcgctcgaa gcgcgcgcgt
     4621 tgcgctcggc cgagctgagc ggaagcgcgg cgctgggcgc cggcgacgtc gccctggcca
     4681 tgggcgagga catcgagcgg ctggccgagg aaacccggcg caaccggtcg gccgcccaga
     4741 tcgcggccga gctcgccgcg ctcggcccag cgccgccgcg tccggcgccg gccgagggcc
     4801 ggcccgcgca gccgccgctc gactatcggc tgccgtcggc cgcccgcgtc gtcgaaggcc
     4861 tgggcgaggt caattcgagc ggcgtacgct cgcgcggcct cacgctcgcc acccgccggg
     4921 ggaccgaggt cgtcgtcccg gccgccggga tcgtccgctt cgccgggccg ttccgggatt
     4981 atgacggaat cgccatcatc gaccatggcg gcggctggat gagcctgatc gtcaacgtcg
     5041 cctcgccgct gaggccgggg gcgaaagtgg ccgccggcga gcgcctcggc cgggcgctcg
     5101 ggccgctcgg ggtcgaattg tcccaccaag ggcgccgcgc ttccccggcc ctaatcgcag
     5161 gttcatctgc gactctgtca aataggccaa aaggcggcta gtctggagcc gcccgaacag
     5221 gacgcgaccg atgaccatga cccgcaaact gcttccaccg ctcgccctag tcggcgcgct
     5281 cgcgctggtc ccggtgacca ccagctcgct cgccgcggcc gatgtcgcca attacgagga
     5341 gctcgagacg ttcatgagcg tctacgagcg ggtcaaggcc aattacgtcg acccggtcga
     5401 cgaccatacg ctgatcaagg gcgcgatcga cggcatgctc gccgcgctcg acccgcattc
     5461 ctcttatgtc gaggcgtccg acttccagac gctccggacc accaccgacg gcaattacgg
     5521 cggcctcggc ctgaccgtct cgaccgagga cggggcggtc aaggtgatct cgccgaccga
     5581 ggacacgccg gcgtggcgcg cgggcatcaa ggccggcgac ttcatcaccc acatcaacag
     5641 cgaactgctt tacgggctga cgctcgacga agcggtcgac aagctgcgcg gaccgcccgg
     5701 gagcgcgatc aaggtgacca tcgtccgccc cggccgcgac aagccgttcg acgtcaccat
     5761 ggtgcgcgag cgaatcgtcc ttcggccggt caagtgggag atcaaggacg gcgtcggagt
     5821 gatcaacatc aactccttcg cggccaatac cggcgatgcc accagggcgg cgctggtggc
     5881 gatcgacaag gcggccggcg gccgaccgat cggctatgtc gtcgacctgc gctcgaaccc
     5941 cggcgggctg ctcgaccagg cgatcgaggt cagcgacacc ttcctcaacc acggcgagat
     6001 cgtttcgcag cgcggccgcg agaaggacga catcgagcgc tattacgcca agcccggcga
     6061 cctcgcgcac ggcctgccgg tggttatcct ggtcgacgcc gggagcgcct cggcggccga
     6121 gatcgtcgcc ggcgctctcc aggaccaccg ccgggcgatc gtcatgggcg agcgcagctt
     6181 cggcaagggc tcggtccaga cggtcgtcca gactggcccg gacagcgcgc ttcggctgac
     6241 cacggcgcgc tattacacgc cgtcgggcaa gtcggttcag gccggcggca tcgatcccga
     6301 catcgtcgtc ccgcagctca ccgaccctga ctacaaggac cggccgcggg ttcgcgaggc
     6361 ggaccttcgc cgccacctga tcagccaggc caaggccgac gacaagctgc tcgagcatga
     6421 cgagacgacc gacccgcgct tcgccgcgac cgcggccgag ctcgagaaga agggcgtcaa
     6481 ggacttccag ctcgaatatg cgattcggac cctgaagcgg ctcgctccgg gcaccgcagc
     6541 gatcgcctcg gccggcggcc ggccgaagac gcgctaagcc cggccgaatg agcgaggcca
     6601 ccctcggccg gcggccggcg ccgctcgccc agtccgggcc ggcgctcgcc cgctggatcg
     6661 cgctgctgct tccgctggcg ctgctcggcg gagcgctcgg ctcgcaatat ctcggcgggc
     6721 tggccccgtg cgagatgtgt tactggcagc gctggccgca cggcgccgcg atcgcgctcg
     6781 ccgggctggc cttcgccggc ccgccggctt cggggcgatc acggacgctg accctgctcg
     6841 ccgcgctcgc catcgccgtc tcgggagcga tcggagtcta ccacgcgggt gtcgagctcg
     6901 gcgtcttcga aggactgacg acctgcgccg cgggcgtcag cggggcgacc aatgccgagc
     6961 ttctcgacca gatcatgaag actccgctgg tccgctgcga ccaggtgcag ttcgccttcc
     7021 tcggcatttc gatggccgga tggaacgcga tcctgtcgct cggcggagcg gcgctgatcg
     7081 cctggctgac gctggggagg cgggcggcgt gagcggcttg cggccgggag agcggcgtcc
     7141 cgaccgggcg tcgatgctgc gggtcaacca ggccggcgaa tatggagcga cgcgcatcta
     7201 tgccggccag ctcgcggtgc ttcgcggcaa ttcgccggca tcgaagctga tcgcccggat
     7261 ggccggccag gaacggcgcc acc
//
LOCUS       DASTZO010000234         6926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_97304,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000234 DASTZO010000000
VERSION     DASTZO010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97304"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..600)
                     /locus_tag="VFU20_06545"
     CDS             complement(7..600)
                     /locus_tag="VFU20_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU5286152.1"
                     /translation="MKTILTIAALAASALAVPSAALAQRAPAAVVVVVDSDRIYRDCT
                     ACKTAQTQLQARVTALQTRQQTLANQLRPEGQAIQTAINALAGKAPDAALRARAEAFQ
                     KKQEEAAQELSRSQQNLQSIQANVGRQINARLNPVINQVMTARGANIAVDVDATLAHS
                     QAVDVTAEVLAGLNRALPSVSLTPLPTQPQPQQPQGR"
     gene            complement(600..3158)
                     /gene="bamA"
                     /locus_tag="VFU20_06550"
     CDS             complement(600..3158)
                     /gene="bamA"
                     /locus_tag="VFU20_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEU5286153.1"
                     /translation="MLLVGTILGGWSAPILAQAATAPPEAAAPAPAARSGIIRSIAVR
                     GNERLEPETVRAYANLNPGDTYTAATLDTALKDLYATELFADVVITGAETGHLVITVR
                     ENPVINRIVLEGNKRLKTDKILPEIKLAPRQIFTRSKVRADVARIVELYRREGRFAAR
                     VEPKIVQLDQNRVDLVFEIFEGDKSKVRRINIIGNNEFPDDRLRKEMFTRQAGGLLGF
                     MKSNDSYDPDRLAADQQKLRAFYLTQGYADFRVVSALAELTPDRRDFIITYVVEEGPR
                     YRLGRVEAESALRDLSPEKIKEVADLKPGEWFNAKAIEDAVVDLTELAGNYGYAFADI
                     NPAYERDSAKREMNITLRVGDTPRVYVERIEIQGNTVTRDKVIRREFRLNEGDAFSAI
                     KVKRSQDRLQSLGFFQEKLEVKQTEGSAPDRIVLGVDVEEKSTGELQLTGGYSSLEKF
                     VLQLAVAQRNFMGKGQELNASVNYSRYAKSVQLGFVEPYLFDKQILLGGDIYRRDLNS
                     FNFVGGERNKTYSQVSTGMGARLGFPITEFVNFGTKYSFVVDKITLDKGTFYTDPDGD
                     TGPLEPRCDPRLAGQYLCDEIGSRTTSAFGYSLLHDDTDGIRPTRGQRISISQDFAGL
                     GGDVKYLRSRASGTKYIEVRDGWVVSAHGEGGYIHALQNSPGPGRDAVRITDRFFGPQ
                     LRGFDIRGIGPRVVRTPYNLDGTLDEEGDQASDALGGRAYYMGRLELEFPTSASIRSM
                     GLRPSAFVDVGSVWSLKKPLLTDIVALCQPASGTPRQVLPGDPNPDCATGETRIPGIR
                     EEFLGNSPKPRLAVGVGVNWVSPFGPFRIDIAKALLSQEGDDTKLFSFNVGTQF"
     gene            complement(3277..4407)
                     /gene="rseP"
                     /locus_tag="VFU20_06555"
     CDS             complement(3277..4407)
                     /gene="rseP"
                     /locus_tag="VFU20_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU5286154.1"
                     /translation="MLQQPPLWFILIAFICALGPLVFIHEFGHYAVAKLFRVGAEVFS
                     IGFGREIAGWTDRSGTRWKVGWLPLGGYVRFVGDMNPASAPAPDADVPEELRSRSFHH
                     KPVWQRFLIVLAGPMANFLLAILIFAAFFAAFGFPRTPAVVASVAPASSAQHAGLVPG
                     DRILAVDGRSTDSFEDLAGYVVLRPDEQIRVEIERDGERRQVSIRLAREVETDRFGQR
                     FEIGRLGVYAEGREYVRLHPLELLPTATSYTVQLTRSMIDGLWQIISGRRSVKDLGGP
                     LKIAQIAGQQASLGAFEFIQLLAFFSINLGFINLLPVPMLDGGHLLFYSVEAVRRRPV
                     SLQAQDWAFRGGLAFILALLLFTTVNDLDSFGLWDRLERLIG"
     gene            complement(4407..5567)
                     /locus_tag="VFU20_06560"
     CDS             complement(4407..5567)
                     /locus_tag="VFU20_06560"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU5286155.1"
                     /translation="MKRKVSILGATGSVGTSTLDLIERSPERFDVVALTAGTDTQGLA
                     DAARRTGARLAVIADAGRLAELEAKLAGTDCRAATGAEALDEAAAGEADWVMAAIVGC
                     AGLVPVMAALEAGRTVALANKEALVSAGALMTGAAARSGATILPVDSEHNAIFQCLAG
                     SSIDQVSKLILTASGGPFRTSSEPEIRAATPAQAVAHPNWSMGAKISVDSATLMNKGL
                     ELIEAHHLFGLPSDRIEVLIHPQSVVHSLVEFIDGSVLAQLGSPDMRVPIAYALAWPE
                     RMATPAERLDLAGIARLEFEKPDLQRFPALRMARQALEQGGAAPTILNAANEVAVACF
                     LAGRIGFTEVAELVEQALGRATLPAPGSIEEVIAIDLETRNAVGALIDARCH"
     gene            complement(5564..6196)
                     /locus_tag="VFU20_06565"
     CDS             complement(5564..6196)
                     /locus_tag="VFU20_06565"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU5286156.1"
                     /translation="MSELFVRSLAAIAMMLVALAAAVLGGYYFAILVAAAATAIFYEW
                     TRIVRGWGPAWAVGGFVYALIPALALLWVRDRADQGLALVLWIFTVTWATDIGAYFVG
                     RAFGRAKLAPSISPGKTVAGLYGGMAGAAVLGGAWVLFAGLNPLLFVLAPVFALAAQG
                     GDLFESWMKRRAGVKDSGHWLPGHGGLLDRLDGLVPVAILTALAQQAGAA"
     gene            complement(6193..>6926)
                     /locus_tag="VFU20_06570"
     CDS             complement(6193..>6926)
                     /locus_tag="VFU20_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEU5286157.1"
                     /translation="SAGLASPEGGDGGVPRHVAIIMDGTGRWADQRGLPRVAGHRAGA
                     EAVRRTLRAAAECGVEALTLYAFSSENWRRPEGEISDLKGLLGYYLERELDALAKEGV
                     RLKLIGDYGAFGPDLVARLDRAVERLAANRRLTLVVALNYGSRGEIAGAARALARKAR
                     DGALDPESIDEEALAAELQTAGLPELDLLIRTSGEVRLSNFLLWQSAYSEMLFTPVLW
                     PDFDEAAFKAAVAEFAARQRRFGGR"
BASE COUNT         1128 a         2307 c         2413 g         1078 t
ORIGIN      
        1 ctcggctcac cgaccctgcg gctgctgcgg ttgcggttgc gtcggcagcg gagtcaggct
       61 gaccgaggga agcgcgcggt tgagcccggc caagacctcg gcggtgacgt cgaccgcctg
      121 cgaatgggcg agcgtggcgt cgacatcgac cgcgatattg gcgccgcggg ccgtcatcac
      181 ctggttgatc accgggttca gccgcgcgtt gatctggcgc ccgacattgg cctggatcga
      241 ctggaggttc tgctgggagc gcgaaagctc ctgggcggcc tcctcctgtt tcttctggaa
      301 tgcctcggcc cgggcgcgaa gcgccgcatc cggcgctttt ccggcaaggg cattgatggc
      361 cgtctggatc gcctgaccct cgggccgcag ctggttggcc agcgtctgct gccgggtctg
      421 gagggcggtc actcgggcct ggagctgggt ctgcgcggtc ttgcaggccg tgcagtcgcg
      481 gtagatccgg tcgctgtcga caacgacgac caccgccgcg ggcgcgcgct gggcgagggc
      541 agcggagggg acggcgagcg cgcttgcggc aagcgctgcg atggtcagga tggtcttcat
      601 cagaattggg ttcctacgtt gaagctgaac aatttcgtgt cgtcgccttc ctgcgaaagc
      661 agggccttgg cgatgtcgat ccggaacggg ccgaacgggg aaacccagtt gacgccgact
      721 ccgacggcga gccggggctt gggcgagttg cccaagaact cctcgcggat cccggggatt
      781 cgggtctctc cggtcgcgca gtccgggttc gggtcgcccg gaaggacctg cctcggagtg
      841 ccgctcgccg gctggcaaag ggcgacgatg tcggtcagca gcggcttctt cagcgaccac
      901 accgagccga catcgacaaa ggccgacggt cggagcccca tgctgcggat cgacgcgctg
      961 gtcgggaatt cgagctcgag ccggcccatg taataggcgc ggccgccgag cgcgtcactg
     1021 gcctggtcgc cctcctcgtc gagagtcccg tcgaggttgt acggggtccg gacaaccctc
     1081 gggccgatcc cgcggatatc gaagccgcgc agctgcggcc cgaagaagcg gtcggtgatc
     1141 cggaccgcgt cgcgccccgg gccgggcgaa ttctggagcg cgtggatata gccgccctcg
     1201 ccatgggccg acacgaccca gccgtcgcgg acctcgatat acttggtccc gctggcgcgg
     1261 ctgcgcagat atttcacgtc gccgccgagg cccgcgaaat cctgcgagat ggaaatccgc
     1321 tggccgcgcg tcgggcgaat tccgtcggta tcgtcgtgaa gcagcgaata gccgaacgcc
     1381 gaagtggtcc gggacccgat ttcgtcgcac agatactggc cggcgaggcg cgggtcgcag
     1441 cgcggttcga gcggcccggt gtctccgtcc gggtcggtgt agaaggtccc cttgtcgagc
     1501 gtgatcttgt cgacgacgaa agagtatttg gttccgaagt tcacgaactc ggtgatcggg
     1561 aaccccagcc gcgcgcccat cccggtgctg acctgggaat aggtcttgtt ccgctcgccg
     1621 ccgacgaagt tgaagctgtt gaggtcacgc cggtagatat cgccgccgag caggatctgc
     1681 ttgtcgaaca gataaggctc gacaaagccg agctggaccg acttggcgta gcgcgaatag
     1741 ttgacgctgg cgttgagctc ctggcccttg cccatgaagt tgcgctgcgc caccgccagc
     1801 tgaaggacga atttctcgag gctcgaatag ccgccggtca gctgcagctc gccggtcgat
     1861 ttctcctcga catcgacgcc gagcacgatc cggtcggggg ccgatccctc ggtctgcttg
     1921 acctccagct tctcctggaa aaagccgagg ctctggaggc ggtcctgcga gcgcttgacc
     1981 ttgatggcgc tgaacgcatc gccctcgttc agccggaatt cgcggcggat caccttgtcg
     2041 cgggtgacgg tgttgccctg gatctcgatg cgctcgacat agacccgcgg agtgtcgccg
     2101 acgcgcaggg tgatgttcat ctcccgcttg gcggagtcgc gctcgtaggc cgggttgatg
     2161 tcggcgaacg catagccgta attgccggcg agctcggtca ggtcgacgac cgcgtcctcg
     2221 atcgccttgg cgttgaacca ttcgccgggc ttgaggtccg cgacctcctt gatcttctcc
     2281 ggagaaagat cgcgcagcgc gctttccgcc tcgacccggc cgagccggta gcggggcccc
     2341 tcctcgacca cgtaggtgat gatgaaatcg cgccggtccg gggtcagttc ggccagcgcc
     2401 gagacgaccc ggaagtccgc atagccctgg gtcaaataga aagcgcgaag cttctgctgg
     2461 tcggcggcca gccggtccgg atcgtagctg tcgttggact tcatgaagcc gagcagcccg
     2521 ccggcctggc gagtgaacat ctccttgcgc agccggtcgt cggggaattc gttgttgccg
     2581 atgatgttga ttcggcggac cttcgacttg tcgccctcga aaatctcgaa caccaggtcg
     2641 acccggttct ggtcgagctg gacaatcttc ggctccactc gggcggcgaa gcggccttcg
     2701 cgccggtaga gctcgacgat ccgcgcgacg tcggcgcgga ctttcgagcg ggtgaagatc
     2761 tggcgcggag cgagcttgat ctcgggcagg atcttgtcgg tcttgagccg cttgttgccc
     2821 tcgaggacga tgcggttgat gaccgggttc tcgcgcaccg tgatcaccag atggccggtc
     2881 tcggcgccgg tgatgacgac gtcggcgaac agctcggtcg cgtacaggtc cttgagcgcc
     2941 gtatcgaggg tcgcggcggt gtaggtgtca ccgggattga ggttggcgta ggcgcggacg
     3001 gtctccggct cgagccgctc gttgccgcga accgcgatcg agcggatgat cccggaccga
     3061 gccgccggcg ccggtgcggc cgcttcgggc ggggcggtgg cggcctgggc gagaatcggg
     3121 gccgaccagc cgccgaggat cgttccgacg aggagcattg cgcccgcgcg cctgccgata
     3181 atctttgttc ctgaaatcac gcgtccccgc cttttgtgaa atatcgcgtt cgaatgacga
     3241 accggccgat gcatccccag ccctgcccca agcgacctat ccaatcaagc gctcaagccg
     3301 gtcccaaaga ccgaatgagt ccagatcgtt gaccgtcgtg aacagcaaca gggcaaggat
     3361 gaatgccagc ccgccccgga acgcccagtc ctgcgcctgc aggctgaccg gccgccgtcg
     3421 aaccgcctcg acggagtaga ataacaggtg gccaccatca agcatcggga ctggcaacag
     3481 gttgatgaat cccagattaa ttgaaaagaa ggcgagcagc tggatgaact cgaatgcgcc
     3541 gaggcttgcc tgctgtccgg cgatctgagc gatcttgagg ggcccgccga ggtccttcac
     3601 cgagcggcgg cccgagatga tctgccacaa gccgtcgatc atcgagcggg ttagctggac
     3661 agtgtagctg gtcgcggtgg ggagcagttc cagcggatgc agccggacat attcgcgccc
     3721 ctcggcgtag acgcccagcc ggccgatctc gaaccgctgc ccgaagcggt cggtctcgac
     3781 ctcgcgggca aggcggatgg agacctgccg gcgctcgccg tcgcgctcga tttcaacccg
     3841 gatctgctcg tcggggcgga ggaccacgta cccggcgagg tcctcgaagc tgtcggtcga
     3901 gcggccgtcg acggcgagga tccggtcgcc ggggaccagg ccggcgtgct gggcggagct
     3961 ggccggggcg acgctggcga cgaccgcggg cgtgcgcgga aagccgaagg cggcgaagaa
     4021 ggccgcgaag atcaggatcg cgagcaggaa attggccatc ggcccggcca gcacgatcag
     4081 gaagcgttgc cacaccggct tgtgatggaa gcttcgcgag cgaagctcct cgggcacgtc
     4141 ggcgtcggga gccggcgcgc tcgccgggtt catgtcgccg acgaagcgga catagccacc
     4201 gagcggcagc cagccgacct tccagcgggt gccgctgcgg tcggtccagc cggcgatctc
     4261 gcggccgaag ccgatcgaga acacctcggc gccgacccgg aacagcttgg cgaccgcata
     4321 atggccgaac tcgtggataa acacgagcgg ccccagggcg cagatgaacg cgatcaggat
     4381 gaaccagagg ggcggctgtt gaagcatcag tggcagcgtg cgtcgatcaa tgcgccgacg
     4441 gcgttgcggg tttccaggtc gatcgcgatc acctcctcga tcgagcccgg cgccggcagg
     4501 gtagcgcgcc cgagcgcctg ctcgaccagc tccgcgactt cggtaaagcc gatccggccg
     4561 gcaaggaagc aggcgaccgc cacttcgttg gcggcgttga ggatggtcgg cgccgcaccc
     4621 ccttgctcga gcgcctggcg cgccatccgc agcgccggga agcgctgcag gtccggcttc
     4681 tcgaactcga gcctggcaat gccggcgagg tcgagccgct cggccggagt tgccatccgc
     4741 tcgggccagg cgagcgcata agcgatcggc acccgcatgt ccgggctgcc gagctgggca
     4801 aggaccgagc cgtcgatgaa ctcgaccagc gaatgaacga ccgactgcgg atggatcaac
     4861 acctcgatcc ggtcggacgg cagaccgaac agatgatggg cctcgatcag ctcgagcccc
     4921 ttgttcatca gggtcgcgga atcgaccgag attttcgcgc ccatcgacca attggggtgc
     4981 gcgaccgcct gcgcaggcgt cgcggcgcgg atctcgggct cgctcgaggt ccggaacggc
     5041 ccgccgctgg cggtcaggat cagctttgag acctgatcta tgctacttcc cgcaaggcac
     5101 tggaaaatag cgttatgctc gctgtccacc ggcaggatgg tcgccccgct ccgggccgcg
     5161 gccccggtca tcagcgcgcc ggcgctgacc agcgcctcct tgttggcgag ggcgacggtg
     5221 cggccggcct cgagcgccgc catcaccggc accagcccgg cgcagccgac gatcgccgcc
     5281 atcacccagt ccgcttcgcc cgcggcggcc tcgtcgaggg cctcggcgcc ggtcgcggcc
     5341 cggcagtcgg ttccggcgag cttcgcctcg agctcggcga ggcggccggc gtcggcgatg
     5401 acggccagcc tggcgcccgt ccttcgggcc gcgtccgcca gaccctgggt gtcggtgccc
     5461 gcggtcagcg cgacgacgtc gaaacgctcc ggggagcgct cgatcaggtc gagcgtcgag
     5521 gttccgaccg acccggtcgc gccgaggatc gagaccttgc gcttcatgcg gcgcccgcct
     5581 gctgcgcgag ggcggtcagg atggctaccg gcacgagccc gtcgagccgg tcgagcaggc
     5641 cgccgtggcc ggggagccag tggcccgaat ccttcactcc ggcgcgccgc ttcatccagc
     5701 tttcaaacag gtcaccgccc tgggcagcca gagcgaagac gggcgcgagc acgaacagca
     5761 gcgggttgag cccggcgaac agcacccagg cgccgccgag gaccgccgct ccggccatgc
     5821 ccccgtacag cccggcgacg gtctttcccg ggctgatcga cggggcgagc ttggcgcggc
     5881 cgaacgcccg gccgacgaaa taggccccga tgtcggtcgc ccaggtgacg gtgaagatcc
     5941 acaagaccag cgccagcccc tggtcggccc ggtcgcgcac ccacagaagc gcgagcgccg
     6001 ggatcagcgc atagacgaag ccgccgaccg cccaggcggg cccccagccg cggacgatcc
     6061 gggtccactc gtagaagatg gcggtcgccg ccgcggcgac gaggatcgcg aaataatagc
     6121 cgccgagcac cgcagcggcc agtgcgacga gcatcatcgc gatcgccgcg aggcttcgga
     6181 cgaacagctc gctcaccggc caccgaaccg ccgctggcgg gccgcgaact ccgccaccgc
     6241 cgccttgaac gccgcttcgt cgaaatcggg ccacagcacc ggcgtgaaca gcatttcgga
     6301 ataggccgac tgccacagca ggaaattgga caggcgcacc tcgcccgagg tgcggatcag
     6361 aaggtcgagc tcgggcaggc cggcggtctg cagctcggcg gccagcgcct cctcgtcgat
     6421 gctctcggga tcgagcgctc cgtcccgggc ctttcgagcc agcgcccgag ccgctccggc
     6481 gatctcgccg cgcgagccgt agttgagggc gacgaccagg gtcagccggc ggttggcggc
     6541 cagccgctcg accgcccggt cgaggcgggc gacaaggtcc gggccgaacg cgccataatc
     6601 gccgatcagc ttgagccgca ccccctcctt ggcgagcgcg tcgagctcgc gctcgagata
     6661 atagcccagc aggcccttga ggtcggaaat ctcgccttcc gggcggcgcc agttctccga
     6721 cgagaaagcg tagagggtca gcgcttcgac accacactcg gcggccgctc ggagagtccg
     6781 gcggaccgcc tcggcgccgg cccggtggcc ggcgacccgc ggcaggccgc gctggtcggc
     6841 ccagcggccg gtgccgtcca tgatgatcgc gacatggcgc ggcacgccgc cgtccccgcc
     6901 ctcgggcgag gcgaggcccg cggacc
//
LOCUS       DASTZO010000235         3853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_960271,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000235 DASTZO010000000
VERSION     DASTZO010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960271"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VFU20_06575"
     CDS             <1..1002
                     /locus_tag="VFU20_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286158.1"
                     /translation="GGAQRLAMQDKGKGREVKAERRQERVVVRDRADERRTARLDRKD
                     RDVRIAGRNDRRDGDRIRVLDGDEWFVPGRGHPIVGGCPPGLMDKGCMPPGQAKKLVG
                     GLLPAAYRDRLLPLGLRDLFRDDDDVFYRYGDGYLYRVDRDTNLVASLLPLLGAGLMP
                     GQMFPSAFGSHYVPGPYQAFYPDTPDDYYRFANGYVYEIDRDTGMIESMVPLMANGYG
                     MGQMLPLSYSAYNVPYQYRGLYPDGGDYMYRYAPGAIYQVDPTTRLITAVASLLTGSG
                     LSVGQPLPMGYGAYNVPLAYRDQYYDTPDAWYRYNNGYIYQVDPTTQLVTALVRAII"
     gene            1083..1652
                     /locus_tag="VFU20_06580"
     CDS             1083..1652
                     /locus_tag="VFU20_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010219341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HEU5286159.1"
                     /translation="MTRTAFALGMAAAALALNACDKADQPGGAPTGEAKDAAGAKTIA
                     AGIDQDSRFLAAAKAAGLDATLAGPGPYTVLVPDDAAFGKLPSGTADNWMKPESRAQL
                     TGVLTNHILPGTILADDIGKAIDNGKGKAILATMGGGTLTATRDGDKIVLTDSAGTKA
                     TVTKADDKHSNGVVHHIDTVLVPSQQGEA"
     gene            1747..2850
                     /gene="dnaN"
                     /locus_tag="VFU20_06585"
     CDS             1747..2850
                     /gene="dnaN"
                     /locus_tag="VFU20_06585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU5286160.1"
                     /translation="MKATIERATLLKSLSHVQSVVERRNTIPILSNVLVEANDDGSIR
                     LMATDLDLQVDESVAANVAQAGATTVSAHTLFDIVRKLPEGSQVELSAAEGKMQVNSG
                     RSRFNLSTLPRDDFPVIAEGELPTRFELPAATLRQVIDKTRFAISTEETRYYLMGIFL
                     HVADDQLKAAATDGHRLARVTVPKPDGAEGMPDVIIPRKCVAELRKLLDEVEGTVEVS
                     LSPTKVRFGLGSAVLTSKLIDGTFPDYNRVIPTANDKLLKLDPKSFMQGVDRVSTIAS
                     EKTRAVKMSLDRDKVTLSVTSPENGLATEELPADYGSDGLEIGFNARYLMDILHEIEG
                     DSVEVHLADAAAPTLLRENDKSNALYVLMPMRV"
     gene            2966..>3853
                     /gene="recF"
                     /locus_tag="VFU20_06590"
     CDS             2966..>3853
                     /gene="recF"
                     /locus_tag="VFU20_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEU5286161.1"
                     /translation="MHVTRLALTDFRSYAAALIEPGPGFVLLSGDNGAGKTNMLEAVS
                     LLTPGRGLRGAALPEMARSGGPGGFSVAARLGETDIGTGALAAAPERRQVRINGASAA
                     VNSLSEWLAVLWLTPAMDRLFADSAGARRRFLDRLVLALEPAHAHHSARYEAAMRARN
                     KLLAEPEGADPAWLAALEAGMAEHGAAIASARARTVEALDERLADAPAGDFAVAKLAL
                     EGWAQGDLAAGLKSSRARDAAAGRATVGPHRQDLAVTHRAKAMPAAQSSTGEQKALLL
                     GLVLAHAELVAGRRGAPPVL"
BASE COUNT          635 a         1378 c         1292 g          548 t
ORIGIN      
        1 ggaggcgccc agcgcctcgc gatgcaggac aagggcaagg gccgcgaggt caaggccgag
       61 cgccgccagg agcgcgtcgt cgtccgcgac cgcgccgacg agcgccggac cgccaggctc
      121 gaccgcaagg accgcgacgt ccggatcgcc ggccggaacg atcgccgcga tggcgatcgc
      181 atccgcgtcc tggacggcga cgaatggttc gtgcccggcc gcggccaccc gatcgtcggc
      241 ggctgcccgc cggggctgat ggacaagggc tgcatgcctc ctggccaggc caagaagctg
      301 gtcggcgggc tgctcccggc cgcctaccgc gaccgactgc tgccgcttgg cctgcgcgac
      361 ctgttccgcg acgacgacga cgtcttctac cgctacggcg acggctacct gtaccgcgtc
      421 gaccgcgaca ccaacctcgt cgcgtcgctg ctgccgctgc tcggcgccgg cctgatgccg
      481 ggccagatgt tcccgtcggc gttcggcagt cactatgtgc cgggcccgta ccaggccttc
      541 tatccggaca ctccggacga ttattaccgg ttcgccaacg gctatgtgta cgagatcgac
      601 cgcgacaccg ggatgatcga aagcatggtc ccgctgatgg ccaatggcta cggcatgggc
      661 cagatgctgc cgctcagtta ctcggcctac aacgtgccgt accagtatcg cggtctctat
      721 ccggacggcg gcgactacat gtaccgctac gctccgggcg cgatctacca ggtcgacccg
      781 acgacccggc tgatcaccgc ggtcgcgtcg ctcctgaccg gcagcgggct cagcgtcggc
      841 cagccgttgc cgatgggcta tggcgcgtac aacgtcccgc tcgcctatcg cgaccagtat
      901 tacgacactc cggacgcctg gtaccgctac aacaacggct acatctacca ggtcgacccg
      961 acgacgcagc tggtgaccgc gctggtccgg gcgatcatct agaaaataat catggatcgc
     1021 ggatggcccg gcatgcgttg ccgggccatt tcgcaggcta aacaagcagg aggatcttcg
     1081 ggatgaccag aaccgcgttc gcgctcggaa tggcggcggc ggctctcgcc ctcaacgcgt
     1141 gcgacaaggc cgaccagccg ggcggcgcgc caaccggcga ggccaaggac gcggccggcg
     1201 ccaagaccat cgccgccggg atcgatcagg acagccgctt ccttgcggcc gccaaggccg
     1261 ccgggctcga cgcgaccctc gccggtcccg gcccctacac ggtgctggtc ccggacgacg
     1321 cggcgttcgg caagcttccg tcgggcaccg ccgacaattg gatgaagccc gaatcgcgcg
     1381 cccagctgac cggcgtcctc accaaccaca tcctgcccgg aacgatactc gccgacgaca
     1441 tcggcaaggc aatcgacaac ggcaagggca aggcgatcct cgccacgatg ggcggcggga
     1501 cgctgaccgc gacgcgcgac ggcgacaaga tcgtgctgac cgattccgcg ggcaccaagg
     1561 cgaccgtgac caaggccgac gacaagcatt cgaacggcgt cgtccaccat atcgacacgg
     1621 tgctggtgcc ctcgcagcaa ggcgaggcct gagcctcctt tccggctggc gccctttccc
     1681 tctggcaagg gcgtcaacgc tcgctataaa gcgggcgacg cgctgaacgg ggctagggac
     1741 gggacgatga aggcgacgat tgaacgggcg acgctgctca agagcctgag ccacgtccag
     1801 tcggtggtcg agcgccgcaa caccatcccg atcctgtcga acgtgctggt cgaggccaat
     1861 gacgacggct cgatccggct gatggcgacc gacctcgacc tgcaggtcga cgagagcgtc
     1921 gccgccaacg tcgcccaggc cggagcgacc accgtctccg cccacacatt gtttgacatc
     1981 gtccgcaagc ttcccgaggg cagccaggtc gaattgtccg cggccgaggg caagatgcag
     2041 gtcaactcgg gccgctcgcg tttcaacctg tcgaccctgc cgcgcgacga tttcccggtg
     2101 atcgccgaag gcgagctgcc gacccgcttc gagcttcccg ccgcgaccct tcgccaggtg
     2161 atcgacaaga cgaggttcgc gatctcgacc gaggagacgc gctactatct gatgggcatc
     2221 ttcctccatg tcgccgacga ccagttgaag gccgcggcga ccgacggcca ccggctcgcg
     2281 cgcgtcaccg tgcccaagcc cgacggcgcc gaaggcatgc cggacgtgat catcccccgc
     2341 aaatgcgtcg ccgagttgcg caagctgctc gacgaggtcg agggcacggt cgaggtgtcc
     2401 ctgtcgccga ccaaggtccg cttcgggctt ggcagcgcgg tcctcaccag caagctgatc
     2461 gacggcacct tccccgacta caatcgggtg atcccgaccg ccaacgacaa gcttctgaag
     2521 ctcgacccga agagcttcat gcagggcgtc gaccgggttt cgaccatcgc gtcggaaaag
     2581 acccgcgcgg tcaagatgag cctcgaccgc gacaaggtga cgctgtcggt gaccagcccc
     2641 gagaacggcc tcgcgaccga ggagctgccg gccgactacg gctcggacgg gctcgagatc
     2701 ggcttcaacg cgcgttatct gatggacatc ctccacgaga tcgagggcga cagcgtcgag
     2761 gtccatctcg ccgacgccgc cgctccgact ctgctgcgcg agaacgacaa gtcgaacgcg
     2821 ctctacgtgc tgatgccgat gcgggtgtga cccgccgcga gcagtaagcc ggccaagcat
     2881 tcgtcattgc gagcggagcg cggcaatcca ctttcgcgtc ggctggattg cttcgccgct
     2941 ccgctcctcg caatgacgta gaattgtgca cgtcacccgc ctcgctttga ccgatttccg
     3001 ctcctacgcg gcggcgctga tcgagccggg gccggggttc gttctgctgt cgggcgacaa
     3061 tggcgccggc aagaccaaca tgctcgaggc ggtgtcgctg ctgactccgg gtcgcgggct
     3121 gcgcggggcg gcgctcccgg aaatggcgcg gagcggcggg cccggcgggt tcagcgtcgc
     3181 ggcgcgcttg ggcgagaccg acatcggcac cggcgcgctg gccgccgcgc ccgagcggcg
     3241 gcaggtgcgg atcaacggcg cttcggcggc ggtcaactcg ctgagcgagt ggctggcggt
     3301 gctgtggctg accccggcga tggaccggct gttcgccgac agtgccggcg cccgccgccg
     3361 cttcctcgac cggctggtgc tggcgctcga gcccgcccac gcccaccaca gcgcgcgcta
     3421 cgaggcggcg atgcgggcgc gcaacaaatt gctcgccgag ccggaaggcg ccgacccggc
     3481 gtggctcgcc gcgctcgagg cggggatggc cgagcacggc gctgcaatcg cttcggctcg
     3541 ggcgcggacg gtggaggcgc tcgacgagcg cctcgccgac gctcccgccg gcgacttcgc
     3601 ggtcgccaag cttgccctag aaggctgggc gcagggcgac ctcgccgcgg gcctgaaatc
     3661 cagccgcgcc cgcgacgccg ccgccggccg ggcgaccgtc ggcccgcacc ggcaggacct
     3721 cgccgtcacc caccgcgcca aggccatgcc cgccgcgcaa agctcgaccg gcgagcagaa
     3781 ggcgctgctg ctcggcttgg tgctcgccca cgccgagctg gtcgcgggcc gccgcggggc
     3841 gccgccggtg ctg
//
LOCUS       DASTZO010000236         2888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1117221,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000236 DASTZO010000000
VERSION     DASTZO010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117221"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VFU20_06595"
     CDS             complement(<1..851)
                     /locus_tag="VFU20_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012817173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HEU5286162.1"
                     /translation="MPIQKLLIANRGEIALRIHRACHEMGIRTVAVHSTADADAMHVR
                     LADETVCIGPPPATESYLNIPNIISAAEIVHADAIHPGYGFLSENAQFAEIVESHDIT
                     WVGPKPEHIRTMGDKIEAKRTAAKLGLPLVPGSDGPLEDIAEAKSLANEIGYPVLIKA
                     ASGGGGRGMKVVPEEEQFESLMSQAASEARAAFGDDTVYMEKYLGDPRHIEFQVFGDG
                     KGQAIHLGERDCSLQRRHQKVLEEAPSPALTQKQRDRMGALVADAMAEMGYRGAGTIE
                     FLYENGE"
     gene            complement(855..1298)
                     /gene="accB"
                     /locus_tag="VFU20_06600"
     CDS             complement(855..1298)
                     /gene="accB"
                     /locus_tag="VFU20_06600"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU5286163.1"
                     /translation="MRIDSRLVQELAELLTANGLTEIEVADGERRIRVGRGHAPAVAP
                     PAAAQAPAASAVAGKQPVAKPAEEVSGETVKSPMVGTAFLSPEPGAEPFIKVGEPVKA
                     GDTLLIIEAMKVMNPITAPSGGVVKKVLVADAQPVEFDQPLVVLG"
     gene            1434..2450
                     /gene="thiS"
                     /locus_tag="VFU20_06605"
                     /pseudo
     CDS             1434..2450
                     /gene="thiS"
                     /locus_tag="VFU20_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
     gene            complement(2443..>2888)
                     /locus_tag="VFU20_06610"
     CDS             complement(2443..>2888)
                     /locus_tag="VFU20_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286164.1"
                     /translation="VRANAEARRVLRDGGRYMLVIWDRVERNLATRAAGEAVAHLFPD
                     DAASFYERIPFRYHDPAVIGHDLLAAGFDDIEFETVELRSRAPSAAAAAKGLCQGSPM
                     RTEIEKQGPDALERATAAAEQALRRFEGPDGLDAPMSAHIVTATR"
BASE COUNT          398 a          972 c         1028 g          490 t
ORIGIN      
        1 aattcgccgt tctcgtagag gaactcgatc gttcccgcgc cgcgatagcc catctccgcc
       61 atcgcatcgg cgaccagcgc gcccatcctg tcacgctgct tctgggtgag cgcgggggag
      121 ggtgcttcct ccagcacctt ctggtggcgg cgctgcaacg agcagtcgcg ctcgccgagg
      181 tggatcgcct ggcctttgcc gtcgccgaac acctggaact cgatgtggcg gggatcgccg
      241 agatattttt ccatgtagac ggtgtcgtcg ccgaaggcgg cgcgcgcttc cgacgcggcc
      301 tggctcatca ggctttcgaa ctgctcttct tcgggcacga ccttcatgcc gcggccgccg
      361 ccgcccgagg ccgccttgat caggactgga tagccgatct cgttcgcaag gctcttagcc
      421 tcggcgatgt cctcgagcgg accgtcggag ccggggacga gcggaaggcc gagcttcgcg
      481 gcggtgcgct tcgcctcgat cttgtcgccc atcgtgcgga tatgttcggg cttcgggccg
      541 acccaggtga tgtcgtggct ctcgacgatc tcggcgaact gcgcgttttc ggagaggaag
      601 ccgtaaccgg ggtggatcgc gtcggcgtgg acgatctcgg ccgccgaaat gatattgggg
      661 atgttgaggt aggattcggt cgccggcggc ggtccgatgc agacggtctc gtcggcgagg
      721 cggacgtgca tcgcgtcggc gtccgcggtc gagtgcacgg cgacggttcg gatgcccatc
      781 tcgtggcatg cgcggtggat cctgagcgcg atctcgccgc ggttggcaat cagcagtttc
      841 tggatcggca tcgcttaacc gagcacgacc agaggctgat cgaactccac gggctgggcg
      901 tcggcgacga ggaccttctt gaccaccccg ccgctcggcg cggtgatcgg gttcatgacc
      961 ttcatcgcct cgatgatcag caaggtgtcg cccgccttga ccggctcgcc gaccttgatg
     1021 aacggctcgg caccgggctc gggcgacagg aaggcggtgc cgaccatcgg cgacttgacg
     1081 gtctcgccgc tgacctcttc ggccggcttg gcgaccggtt gcttcccggc gaccgcggac
     1141 gccgcgggtg cttgagccgc cgccggcggg gcgaccgccg gcgcgtggcc gcggccgacc
     1201 ctgattcggc gctcgccgtc ggcgacctcg atctcggtca gcccgttggc ggtcagcaac
     1261 tcggcgagct cctgcaccag cctggagtcg atgcgcatcg atcgctcctt ggcctcgtcc
     1321 cctttggcgg agctcgggac gggtttgccg gattcggcca ttgcagcggt cccctgttaa
     1381 ttctggccag tcgtttgcgc ttggccgtgg agcttcctat ctgcgctttg tcaatgacca
     1441 tcgatgccca acagagcctc gcgatccgag tcaatggcga ggagcggcgg gtgcccgccg
     1501 gggtcagcat cgcggagatg ctgcgcgggc tcggcctcga cccgcgtcgg gtcgcggtcg
     1561 agcgcaacct cgaggtcgtg ccgcgctcga ctctcgccga cgtgccggtc gaggacggcg
     1621 accggttcga ggtggtccat ttcgtcgggg ggggctgagt gaccgcccgc ccgagcatcg
     1681 acaccgacag ctggacggtc gccggccgga ccttcacctc gcggctgatc gtcggcaccg
     1741 gcaagtacaa gagcttcgag gagaatgccg ccgcggtcga ggcgtcgggg gcggagatcg
     1801 tcacggtcgc ggtgcggcgg gtcaacatcg ccgacccgaa gcagccgatg ctgaccgact
     1861 tcatcgaccc caggcgcgtc acctatctgc ccaacaccgc cggctgcttc accggcgagg
     1921 aagcggtgcg cacgctgcgc ctggcgcgcg aggccggcgg ctggacgctg gtcaagctcg
     1981 aggtgctcgg tgaggcgaag accctctacc ccgacatggt cgagactttg cgcgccaccg
     2041 agatcctcgc caaggagggc ttcgagccga tggtctattg cgccgacgac ccgatcgccg
     2101 cgaggcggct cgaggacgcc ggcgcggtgg cgatcatgcc gctgggcgca ccgatcggct
     2161 cgggcctcgg cctgcagaac ctggtcacca tccggctgat cgtcgaaggc gcgaacgttc
     2221 cggtgctggt cgatgccgga gtcgggacgg cgagcgacgc ggcggtggcg atggagctgg
     2281 gctgcgacgg ggtgctgatg aacaccgcga tcgccgaggc caaggacccg gtgctgatgg
     2341 cccgggcgat gaaggccgcg gtcgaggccg ggcggctcgc ctacctcgcc gggcgcatgc
     2401 ccaagcgccg ctacgccgat cccagcagcc cgctcgccgg gatcatctag tcgccgtcac
     2461 gatgtgcgcc gacatcggcg cgtcgagccc gtccgggcct tcgaaacgcc gcagcgcctg
     2521 ctcggcggcg gcggtcgcgc gctcgagcgc gtccgggccc tgcttctcga tctcggtgcg
     2581 catcgggctg ccctggcaca ggcccttcgc ggccgcggcg gcggaagggg cgcggcttct
     2641 gagctcgacg gtctcgaact cgatgtcgtc gaaccccgcg gcgagcaggt cgtgcccgat
     2701 gaccgcggga tcgtggtagc ggaacgggat gcgctcgtag aagctcgccg catcgtcggg
     2761 aaacaagtgc gcgaccgctt cgccggcggc cctggtcgcc aggttgcgct cgacccggtc
     2821 ccagatgacc agcatgtagc ggccgccgtc gcggagcact cgccgcgcct cggcgttcgc
     2881 ccggacct
//
LOCUS       DASTZO010000237         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_124648,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000237 DASTZO010000000
VERSION     DASTZO010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124648"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1778)
                     /locus_tag="VFU20_06615"
     CDS             complement(<1..1778)
                     /locus_tag="VFU20_06615"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU5286165.1"
                     /translation="MAYSGVYVFGDSLVDAGNALKLAEWYGDLTFSDLPDGAPTEEEG
                     YFEGRFSNGYTFADLLANKAIGTITEPVFPYGYEDPWLGIPISPWSSDPNGNNLNFAY
                     GGAQIRQGDEVVPDLDGQTDTFRDAVDGDADPNALYLIAIGGNDVRNLAPSGGDPVSP
                     ADGYAALDKAAEKYLTELSQLVAMGARHIVITGVPDVGLIPRYDRDGNLVLDAAELLR
                     SEAATDFSIYLDTLIRTEVVPALQALGATVTYVPLMDYVDSSGNLVTGALNANLATIA
                     ALHGLTADELSDNLLQHQDLLFFDQIHPNAQANALLGAYMHAQLTGTAWVETLPLTGA
                     EVDYRMSGSIASPGEVDKLVVALVAGTTYTLDLLGMSSLGISGSLADPALRLLGPSGT
                     LFGSNADDGAGFDSTLTFTAATTGNYTIEILGTGTPTGSYVLQAAVIGGAATLAGNTY
                     TVSNAATVVLEGAGGIGQDVVKASVSYALSEGSEIEVLRTTHDKGKTAINLTGNEFDQ
                     TIVGNSAANVLEGKAGADVLVGGAGKDVFVLSNAAVTDPGAANIDTISDYAAGDVVDV
                     TQILSVAAGTDVLAGGFLRVTTGGLV"
     gene            2046..2333
                     /locus_tag="VFU20_06620"
     CDS             2046..2333
                     /locus_tag="VFU20_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286166.1"
                     /translation="MARTNALWRTYFDAGEIGTPTGRAIVEERFAALRRQIPIIYLLA
                     LINLFGLQLAATGAVTLGFNPPTFVAVCALVRMGQWLQAPKSVSTRSCSGA"
     gene            2318..>2972
                     /locus_tag="VFU20_06625"
     CDS             2318..>2972
                     /locus_tag="VFU20_06625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5286167.1"
                     /translation="MLRRLRQTLWTAAIICSAICIWCLQLLQQGPDTRLAVLLFGSMT
                     AIGAAYGLSSFPAAARLPLLVLAFPLAGEALLSDDTQFVGGALSLSIVALLILRLLNV
                     HNRHFTDLIRSRATIASAQERAENARREANQAATTDFLTGLPNRRAFVAAIDSELKRK
                     DRPGRFTVAIVDLDRFKPINDTFGHATGDELLCTVARRLVEAVGDEAIVARLGGDEFA
                     "
BASE COUNT          460 a          992 c         1020 g          500 t
ORIGIN      
        1 tggacgagcc cgccggtcgt cacccgcaga aagccgccgg ccaggacgtc ggtgcccgcc
       61 gcgacgctca ggatctgggt gacgtcgacg acgtcgccgg ccgcatagtc ggagatcgtg
      121 tcgatgttgg ccgcgccggg atcggtcacc gcggcgttgc tgagcacgaa cacgtccttg
      181 cccgcgccgc cgaccagcac gtcggcgccg gccttgccct cgaggacatt ggccgccgaa
      241 ttgccgacga tggtctggtc gaactcgttg ccggtcaggt tgatcgccgt cttgcccttg
      301 tcgtgggtgg tccggagcac ctcgatctcg ctgccctcgc tcaaggcgta gctgacgctc
      361 gccttgacga cgtcctggcc gatcccgccg gcgccctcga gcaccaccgt cgccgcgttg
      421 ctgaccgtgt aggtgttgcc ggcgagcgtc gccgccccgc cgatcaccgc cgcctgaagc
      481 acatagctgc cggtcggagt gccggtgccg agaatctcga tggtgtaatt gccggtcgtc
      541 gcggcggtga aggtcagggt cgagtcgaag ccggcgccgt cgtcggcgtt cgatccgaac
      601 agcgtgccgc tcggcccgag cagccgcagc gccgggtcgg cgaggcttcc ggaaatgccg
      661 agcgagctca ttccgagcag gtcgagggtg taggtggtgc cggcgaccag cgcgacgacg
      721 agcttgtcga cctcgccggg cgaggcgatg ctcccactca tccggtaatc gacctcggcg
      781 ccggtcagcg gcagcgtctc gacccacgca gtgccggtca gctgggcgtg catgtaggcg
      841 ccgagcagcg cattggcctg ggcgttgggg tggatctggt cgaagaacag caggtcctgg
      901 tgctgcagca ggttgtccga cagctcgtcc gcggtcagcc cgtggagcgc ggcgatggtg
      961 gcgaggttgg cgttgagggc gccggtgacg aggttccccg acgaatcgac ataatccatc
     1021 agcggcacgt aggtgacggt cgcgccgagc gcctgcagcg ccggcaccac ctcggttcgg
     1081 atcaaggtgt cgagatagat cgagaaatcg gtcgccgctt cgctgcgcag cagctcggcc
     1141 gcatcgagga cgaggttgcc gtcgcggtcg tacctcggga tcaggccgac gtccggaacg
     1201 ccggtgatga cgatgtgccg ggcgcccatg gcgaccagct ggctcaactc ggtgagatac
     1261 ttctcggccg ccttgtcgag cgccgcatag ccgtccgccg gcgagaccgg gtcgccgccg
     1321 ctgggcgcca ggttgcgcac gtcgttgccg ccgatggcga ttaggtagag ggcattaggg
     1381 tcggcgtcgc cgtcgaccgc gtcgcggaag gtgtcggtct gcccgtcgag gtcggggacg
     1441 acttcgtcgc cctggcggat ctgcgctccg ccataggcga aattgaggtt gttgccgttg
     1501 gggtccgacg accagggcga gatcgggatt cccagccacg ggtcctcgta gccgtagggg
     1561 aagaccggct cggtgatcgt tccgatcgcc ttgttggcga gcaggtcggc gaacgtgtag
     1621 ccgttggaaa agcggccctc gaaatagccc tcctcttcgg tcggggcgcc gtcgggcagg
     1681 tcgctgaagg tgaggtcgcc gtaccattcg gcaagcttga gagcattgcc ggcatcgacc
     1741 aggctgtcgc cgaacacata cacgccgctg taagccatgg ttcacccccg aaccggtgca
     1801 gcgagcgccg ctgcgactcg ggccccatcg acccgatttc gaaggctgcc ataaattatc
     1861 ggaatatcaa cggcatgaat agttaacgca gccgcaggcc gtggcgctag gacccgcgtg
     1921 cagcccgcgc ccgagccccg aggaaccgct cggccaggcg cgtgaccgag gctgcgcgag
     1981 cgccgccatt gccggccggg gagcgcgtca acgcggtctt aagctaatcg cttcaaaaag
     2041 cggcaatggc tcggacgaac gcgttgtggc ggacctattt cgacgcgggt gaaatcggga
     2101 ctccgaccgg ccgggcgatc gtcgaagagc gcttcgcggc gcttcggcgg caaatcccga
     2161 tcatctacct gctggcgctg atcaacctgt tcggcctgca gctcgccgca accggcgccg
     2221 taaccttggg attcaacccg ccgaccttcg tcgcggtttg cgcgctggtc cgcatgggac
     2281 agtggctcca ggcgcccaaa agcgtgtcca cgaggtcatg ctccggcgcc tgaggcagac
     2341 cttgtggacc gcggcgatca tctgttcggc gatctgcatc tggtgcctgc agctcctcca
     2401 gcaaggcccc gacacgcggt tggcggtgct cctgttcgga agcatgaccg cgatcggtgc
     2461 ggcctacggc ttgagcagct tcccggccgc ggctcggctt ccgctgctgg tcctggcctt
     2521 tccgctcgcg ggcgaagccc tgctttcgga cgacacccag ttcgtcggcg gcgcccttag
     2581 cctttcgatc gtcgcgctgc tcatcctgcg cctgctcaac gtgcacaacc ggcacttcac
     2641 cgacctgatc cggtcgagag cgaccatcgc cagcgcccag gagcgcgccg agaacgcccg
     2701 tcgggaagcg aaccaggcgg cgaccaccga cttcctgacc ggcctaccca accggcgcgc
     2761 cttcgtcgcc gcgatcgact cggagctcaa gaggaaagac cggccgggcc ggttcacggt
     2821 ggccatcgtc gatctcgacc gcttcaagcc gatcaacgac acgttcggcc acgccaccgg
     2881 ggacgagttg ctgtgcacgg tcgcccgtcg cctggtcgaa gcggtcggcg acgaagcgat
     2941 cgtcgcgcgc ctcggcggcg acgagttcgc cg
//
LOCUS       DASTZO010000238         2128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_150787,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000238 DASTZO010000000
VERSION     DASTZO010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150787"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(484..1260)
                     /locus_tag="VFU20_06630"
     CDS             complement(484..1260)
                     /locus_tag="VFU20_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5286168.1"
                     /translation="MSGVNWVGLKTLYIKEVRRFFKVHMQTVWAPAITTLLYLVIFTV
                     ALGGSGRTVMGVRFADFLAPGLIVMAMLQNAFANASFSLLVGKMQGNIVDYLLPPLSV
                     GELIAGLVGAAVTRAFLVGFAVWLAMLAWPDVSVAVREPLWLLWFALMGSLMLSFLGL
                     MTSIWADKFDHAAAVTNFVVTPLSLLSGTFYSVEQLSPAFQAVSHANPFFYVISGFRY
                     GFLGVADSPLWVGSAVLLLLNIALWAACYALLKSGWKIKN"
     gene            1414..2058
                     /locus_tag="VFU20_06635"
     CDS             1414..2058
                     /locus_tag="VFU20_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HEU5286169.1"
                     /translation="MSWTDERIERLKKMWADGATASQIADELGGVSRNAVIDKAHRLG
                     LDARPSPVKPGDEKEKKPAASAARPARADASARRAHPAPEDGATSAAPARTPQRVQPE
                     GIQYRSIGPGGFVRQGPGDTQAPIPPAPPRRLVPAKPSAEVADKTSLLDLNDRICKWP
                     LGHPGEPDFHFCGAPANPGFPYCVEHCGVAYQAQLPRRDRRPPPPLPFGGPRVR"
BASE COUNT          389 a          759 c          689 g          291 t
ORIGIN      
        1 gtggggcatg atcctgcggt aaacgacgcc ttccactccg tcgaagcgcc aacctttggt
       61 tgcgcgcccg caatcacccc accataatcc cccgcgcagc caccacgcac ggctgcccga
      121 ttacggtgac agtttactaa atgcaccaat tgcaacggcg aaccgccgcg caaatttccc
      181 tgtcctacag gccgcagagt gtgagacagt cccgactcgc ccggctgggc tgctctttga
      241 gattgtcgat cctccacctg cctgtcccga gcgcgtcgag gggcctgtcc tgagcttggt
      301 cgaagggcgg gccgcctgcc cgccgccgtt ctccgcttga gctggcgccg catcccattg
      361 ttttcccaat gaaaaagatc ggccaggaaa ccgctgcagc gtccgctttt tgcgggccgc
      421 cgccccaccg ccgaccgcgg tcgatatggg tgagcttcgt taggatccag ttccaggaac
      481 ggctcaattc ttgatcttcc agccgctctt gaggagcgca tagcaggccg cccacagagc
      541 gatgttgaga agcaggagca cggcgctgcc gacccacagc ggcgaatcgg ctacgcccag
      601 gaagccgtag cggaagcccg aaatcacgta gaagaacggg ttggcgtggc tgaccgcctg
      661 gaacgccggc gacaattgct cgaccgagta gaaggtcccg gacagcagcg acaagggcgt
      721 gacgacgaag ttggtgaccg ccgccgcatg gtcgaacttg tcggcccaga tcgacgtcat
      781 gaggccgagg aacgagagca tcagcgagcc catcagggcg aaccacagca gccacaaggg
      841 ctcgcggacg gcgaccgaca cgtcgggcca ggcgagcatc gccagccaca ccgcaaagcc
      901 gaccaggaac gcgcgggtga ccgcggcccc gaccagcccg gcgatcagct cccccaccga
      961 cagcggcggc agcaggtaat cgacgatatt gccctgcatc ttgccgacca gaagcgagaa
     1021 gctggcgttg gcgaaggcgt tctggagcat cgccatgacg atcagcccgg gcgccaggaa
     1081 gtcggcgaag cggaccccca tcacggtccg gccgctgccc cccagagcga cggtgaagat
     1141 gaccaggtac agcagggtcg tgatcgccgg cgcccacacg gtctgcatgt gcaccttgaa
     1201 gaagcgccgc acctccttga tatagagggt cttgaggccg acccagttga cgccgctcaa
     1261 gaccggcgcg ccgggctcgc tctgaagcca cggcggaggc tggtcgccat gagcctgggt
     1321 attctggggc tggccgttca ccggtcgctc gcctatcggc tggcggcacc cgccgcaagc
     1381 tggggcgttc gaccgcactg gagtagaaat tgcatgtcct ggaccgacga gcgcatcgag
     1441 cggctcaaga agatgtgggc cgatggcgcg accgcgagcc agatcgccga cgagctcggc
     1501 ggggtcagcc gcaacgccgt gatcgacaag gcgcaccgcc tcgggctcga cgcgcgcccg
     1561 tcgccggtca agccgggcga cgagaaagaa aaaaagccgg cggcctcggc ggccaggccg
     1621 gcgcgagccg acgcttcggc ccggcgcgcc catccggcgc ccgaggacgg cgccacctcc
     1681 gccgccccgg cccgcactcc gcagcgcgtg cagccggagg gaatccagta tcgctcgatc
     1741 gggcccggcg gcttcgttcg ccaggggccc ggcgacaccc aggcgccgat tccgccggcc
     1801 ccgccgcgcc ggctggtgcc cgccaagccg agcgccgagg tcgccgacaa gaccagcctg
     1861 ctcgatctga acgaccgcat ctgcaaatgg ccgctgggcc atccgggcga gcccgacttc
     1921 catttctgcg gcgctcccgc caacccgggc ttcccctatt gcgtcgagca ttgcggcgtc
     1981 gcctaccagg cgcaattgcc ccgccgcgac cgccgcccgc ccccgccgct ccccttcggc
     2041 gggcccaggg tgcgctagag ccgccgagca tagtgccgcc gacgcgcaaa gcgcgacgcc
     2101 ggcgctatgt cgagagacgg cgagagcc
//
LOCUS       DASTZO010000239         2793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_751566,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000239 DASTZO010000000
VERSION     DASTZO010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751566"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFU20_06640"
     CDS             complement(<1..430)
                     /locus_tag="VFU20_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU5286170.1"
                     /translation="MAPPRPGWSRRAQYGLFFGFLAVIAGLIVGVFLLALSLIAPKTF
                     QTVRGAALDATAPVSMALHEVTATAEGLVSGAGNYWDAAHQNSKLKRERQAMLRRMIE
                     AKAILQENRQLKAALQLRERNREAVAAGRIVGSSLSSPRRY"
     gene            complement(445..1488)
                     /locus_tag="VFU20_06645"
     CDS             complement(445..1488)
                     /locus_tag="VFU20_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU5286171.1"
                     /translation="MFWARWFKWMSHDMAIDLGTANTLVYVRGRGIVLNEPSVVAIET
                     INGVKRVKAVGEDAKLMMGKTPDQIEAIRPLRDGVIADIDVAEQMIKHFIHKVHGGKM
                     RAWRFPEIVICVPSGSTSVERRAIRDAASNAGASSVYLIEEPMAAAIGADMPVTEPIG
                     SMVVDIGGGTTEVAVLSLRGLAYTTSVRVGGDKMDEAISSYVRRNHNLLIGEATAERI
                     KKEVGIAKPPVDGIGKTVHIKGRDLVNGVPKEISINQGQIAEALSEPVGTIVEGVRIA
                     LENTAPELAADICDQGIVLTGGGALLQGLDEVLRDETGLPVTVAEDPLTCVALGTGRA
                     LEEEQFRGVLQTA"
     gene            1643..>2793
                     /gene="mutL"
                     /locus_tag="VFU20_06650"
     CDS             1643..>2793
                     /gene="mutL"
                     /locus_tag="VFU20_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU5286172.1"
                     /translation="MSITRLPSELIDRIAAGEVVERPASALKELVENALDAGARTVAV
                     RLAGGGTDLIDVVDDGSGMPPAEMALALERHATSKLPDGAIDRVTSFGFRGEALPSIA
                     SVSRLTLESRERGGADAQGGGWRIEIDHGARVGEGPAALPPGTRIRVEGLFARVPARR
                     KFLRSPRAEYAACLDAVKRLAMARSDVAFSLEHDGRRVLSLQPASAPARVAELLSHEL
                     DRHGIGVDCARDGLGLTGVVSLPTFNRGLADQQFLFVNSRPVKDRLLVGALRAAYRDL
                     IARDRHPVAALFLQVPLEDVDVNVHPAKTEVRFRDPSAVRGLIVGGLRRALDEAGHRS
                     AAHEQAAAPVMWTSDLDHRHPGEGRDLGPEVQTLAEARAFAGATGVAEAR"
BASE COUNT          399 a          971 c          987 g          436 t
ORIGIN      
        1 cgtagcgccg cgggctgctc agcgacgagc cgacgatccg gccggcggcg accgcctccc
       61 ggttgcgctc gcgaagctgc agcgcggcct tcagctggcg gttctcctgg aggatcgcct
      121 tggcctcgat catccgccgg agcatcgcct ggcgctcgcg cttgagcttc gagttctggt
      181 gcgccgcgtc ccagtaattg ccggcgccgc tgaccagccc ctcggcggtc gcggtgactt
      241 catggagggc catgctgacc ggggcggtcg catcgagcgc ggcgccgcgg acggtctgga
      301 aggtcttcgg agcgatcagc gacagcgcca gcaggaagac gccgacgatc aggccggcaa
      361 tgaccgcgag gaagccgaag aacagcccgt actgcgcccg tctcgaccaa ccggggcgcg
      421 ggggcgccaa cgctcagcct tccgtcaggc ggtttgcagc acgccgcgga actgctcttc
      481 ctcgagcgcg cggccggtgc cgagcgcgac gcaagtgagc ggatcctcgg ccaccgtcac
      541 cggcagcccg gtttcgtcgc gaagcacctc gtcgagcccc tggagcaacg ctccgccgcc
      601 ggtcagcacg atgccctggt cgcaaatgtc ggcggcgagc tcgggcgccg tgttctcgag
      661 cgcgatccgg actccctcga cgatcgtccc gaccggctcc gacagggcct cggcgatctg
      721 cccctggtta atcgagattt ccttgggaac tccgttgacc aggtcgcggc ccttgatgtg
      781 gacggtcttg ccgatcccgt cgaccggcgg cttggcgatg ccgacctcct tcttgatccg
      841 ctcggcggtc gcctcgccga tcaggaggtt gtggttgcgc cggacgtagg agctgatcgc
      901 ttcgtccatc ttgtcgccgc cgacccgcac cgaagtcgtg taggcgaggc cgcgaaggct
      961 cagcaccgcg acctcggtgg tgccgccgcc gatgtcgacg accatcgatc caatcggctc
     1021 ggtgaccggc atgtcggcgc cgatcgcggc ggccatcggc tcctcgatca gatagaccga
     1081 gctggcgccg gcgttcgagg ccgcgtcgcg gatcgcgcgc cgttcgaccg aggtcgaccc
     1141 ggatgggacg cagatgacga tctcggggaa gcgccaggcg cgcatcttgc cgccgtgcac
     1201 cttgtggatg aagtgcttga tcatctgctc ggcgacgtcg atgtcggcga tgaccccgtc
     1261 gcgaagcggg cggatggcct cgatctggtc gggcgtcttg cccatcatca gcttggcgtc
     1321 ctcgccgacg gccttgaccc gcttgacgcc gttgatcgtc tcgatcgcga ccaccgacgg
     1381 ctcgttgagg acgatgcctc ggccgcgcac atagacgagc gtgttggcgg tgccgagatc
     1441 gatcgccatg tcgtgcgaca tccatttgaa ccagcgcgcc cagaacatcc gtcagtgtct
     1501 ctcgttcggt ccgcgattcg aaggttaacg ccgccgccaa cccgattcac gaattgataa
     1561 acccctcgcc gtgaaaaccc tgtaaattcc ggagaaaatc agcgcttcgc ggagtgactt
     1621 cgattgggct aggaccctta acatgtcaat aacgagactg ccgtccgagc tgatcgaccg
     1681 aatcgccgcg ggcgaggtgg tcgagcggcc ggcgagcgcc ctcaaggagc tggtcgagaa
     1741 tgcgctcgac gcgggcgcgc ggacggtcgc cgtccggctc gcgggcgggg ggaccgacct
     1801 gatcgacgtg gtcgacgacg gcagcggcat gccgcccgcc gagatggccc tggcgctcga
     1861 gcgccacgcg acctccaagc tgcccgacgg ggcgatcgac cgggtgacca gcttcggctt
     1921 ccgcggcgag gcgctgccgt cgatcgccag cgtgtcgcgg ctgacgctcg aaagccgcga
     1981 gcggggcggc gccgacgcgc aggggggcgg ctggcggatc gaaatcgacc atggcgctcg
     2041 ggtgggcgag gggccggccg cgcttccgcc ggggacccgg atccgggtcg agggcctgtt
     2101 cgcccgggtg ccggcgcggc gcaagttcct gcgcagcccg cgcgccgaat atgccgcctg
     2161 cctcgatgcc gtgaagcggc tggcgatggc gcggtccgac gtcgccttca gcctcgagca
     2221 tgacggccgg cgggtgctgt cgctgcagcc ggcgtcggcc ccggcgcggg tcgccgaatt
     2281 gctcagccac gagctcgacc gccacggcat cggcgtcgat tgcgcgcgcg acgggctcgg
     2341 cctgaccggg gtcgtcagcc tgccgacctt caaccgcggc ctggccgacc agcagttcct
     2401 gttcgtcaat tcgcggccgg tgaaggaccg gctgctggtc ggggcgttgc gcgccgccta
     2461 ccgcgacctg atcgcccgcg accgccaccc ggtcgccgcg ctgttcctcc aggtcccgct
     2521 cgaggacgtc gacgtcaacg tccacccggc caagaccgaa gtccgcttcc gcgacccgtc
     2581 ggcggtgcgc gggctgatcg tcggcgggct tcgccgcgcg ctcgacgagg ccgggcaccg
     2641 gagcgcggcg cacgagcagg cggcggcgcc ggtgatgtgg acgagcgacc tcgatcatcg
     2701 tcatcccggc gaaggccggg atctcgggcc ggaagtgcag actctggccg aggcccgggc
     2761 cttcgccggg gcaacgggtg tggccgaggc acg
//
LOCUS       DASTZO010000240         3930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_851767,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000240 DASTZO010000000
VERSION     DASTZO010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3930
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851767"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1175
                     /locus_tag="VFU20_06655"
     CDS             <1..1175
                     /locus_tag="VFU20_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEU5286173.1"
                     /translation="EDTVPDVVRARELGLTHLTRPQFLARLLNAAQRSVAVGGTSGKS
                     TVTGMIGWILHACHRQPTVMNGAVMKNFVTPSAPFASALVGDPELFVSEVDESDGSIG
                     LYKPEVAVLTNISLDHKEMAELRSLFAAFLLRARKSVLNLDDPETRALADVVPGEKCV
                     GYGFDSPGAGLMGKQLELLPDGVTFAVEAEGERHEVRLAIPGRHNASNALAALAAARA
                     LGVPLAEGAAALARFEGLTRRLEKVGSAAGVTVIDDFAHNPDKIDATLATLRAQPGRL
                     LILFQPHGFGPLVKMGEELAHSFARGMAPGDRLYLPDPVYHGGTVERRRGSDWLAEAV
                     RAQDRHAEHIPERSAIGEALLAEARDGDRIVIMGARDDSLSEFAAELVERLGQRNS"
     gene            1198..1575
                     /locus_tag="VFU20_06660"
     CDS             1198..1575
                     /locus_tag="VFU20_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286174.1"
                     /translation="MAKPGTVTGWKLPSDERDALLERFPPRYAEVIADHVTLRLGATP
                     ETPLPRKPDARIIGRADDGKSLECLVVELDGTTDRPDGSTYHITWSLGPGRKARESND
                     VLRDLGWEPIGAPIPVELEPARF"
     gene            1577..2071
                     /locus_tag="VFU20_06665"
     CDS             1577..2071
                     /locus_tag="VFU20_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286175.1"
                     /translation="MPGPQPRLFLDADGVLADFDSGAQALLGMTPAEFEARHGRREFW
                     RRIATARNFYGTLPEMPDARRLFDAVEHLKPTILTGLPLGNWAAPQKVRWAAEHFPGV
                     PIITCMARDKHKHMKPGDVLVDDRENHRASYEREGVVFVHHKSAEDSLRQLAQIFPSV
                     KAAA"
     gene            complement(2068..2616)
                     /locus_tag="VFU20_06670"
     CDS             complement(2068..2616)
                     /locus_tag="VFU20_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286176.1"
                     /translation="MTRTSQPASGAMLISEMKEFASFSKATQRYIRRSLDVAYGRRDP
                     IECWARDEGEAASIRAQGRLYKQLDHMRLQVPDDSGLDMIEPFIGMLITVSAFDLGQD
                     RLPHFASYRFLYERLIGASARPWLPSAFCSAAALPHLHPDRRRVLLQSISESAATAPG
                     WSNREPVFWPEWVEKVDVIAAA"
     gene            complement(2815..3030)
                     /locus_tag="VFU20_06675"
     CDS             complement(2815..3030)
                     /locus_tag="VFU20_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286177.1"
                     /translation="MMNGEQKSDVPPPVLSDDPERELEKELRDNPHDLDKKADVGSDE
                     SMDASDPPAAAQPGQNDDPVPSSGFPE"
     gene            3178..>3930
                     /locus_tag="VFU20_06680"
     CDS             3178..>3930
                     /locus_tag="VFU20_06680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5286178.1"
                     /translation="MNNHSEMAAARADSRAGDGGRFSDWPLALKSILGFWLFYALTVV
                     ARALLGADPATVLQNKSVTIVAGIVLTAAIYAAIQLFAARAGLRRRAIVAVAASFIAA
                     GGQAAVLIAADRYQEKPQDEHLYVSREGYRVVELGNAMRIERGGHPPLTVTWPNISSL
                     DPYLQFRIAADSAVVWLFFFAAWSAFYLATLAQAEALGARRRVAEAESAARAAQVRAL
                     RYQVNPHFLFNTLNSLSSLVMTGRTDRAEAMLL"
BASE COUNT          599 a         1398 c         1316 g          617 t
ORIGIN      
        1 tcgaggacac ggtccccgac gtcgtccgcg cgcgcgagct cgggctcacc cacctgaccc
       61 ggccgcaatt tctcgcccgg ctgctcaacg ccgcgcagcg cagcgtcgcc gtcggcggca
      121 ccagcggcaa gtcgacagtg accgggatga tcggctggat ccttcatgcc tgccaccgcc
      181 agccgacggt catgaatggc gcggtgatga agaatttcgt cacgccctcc gcgccgttcg
      241 cgagcgcgct ggtcggcgac cccgagctgt tcgtcagcga ggtcgacgaa agcgacggct
      301 cgatcggcct ctacaagccg gaggtcgcgg tgctgaccaa catcagcctc gaccacaagg
      361 agatggccga gctgcgctcg ctgttcgccg ccttcctgtt gcgtgcgcgg aaatccgtcc
      421 tgaacctcga cgacccggag acccgggccc tcgcggacgt cgtccccggc gaaaaatgcg
      481 tcggctacgg cttcgacagc ccgggcgcgg gcctgatggg caagcagctc gagctgctcc
      541 ccgacggcgt caccttcgcg gtcgaggccg agggcgagcg ccacgaggtg cggctcgcga
      601 tccccggccg ccacaatgcg tccaacgcgc tcgccgcgct ggccgcggca cgcgcgctcg
      661 gagtcccgct cgccgagggg gccgcggcgc tggcccggtt cgagggcctg acgaggcggc
      721 tcgaaaaggt cggcagcgcg gccggagtca cggtgatcga cgatttcgcc cacaacccgg
      781 acaagatcga cgcgaccctg gcgaccctgc gcgcccagcc gggccgcctg ctgatcctgt
      841 tccagccgca cggcttcggg ccgctggtca agatgggcga ggaactggcc cacagtttcg
      901 cccgcgggat ggcgcccggc gaccgccttt acctgcccga cccggtctat cacggcggca
      961 ccgtcgagcg gaggcgcggg tccgactggc tcgccgaagc ggtccgcgcc caggatcgcc
     1021 acgccgagca catccccgag cgctcggcga tcggcgaggc cctgctcgcc gaggcgcgcg
     1081 acggcgaccg gatcgtcatc atgggcgcgc gcgacgactc gttgagcgaa ttcgcggccg
     1141 agctggtcga gcggctcggg caaaggaact catagccggc ttccgcgtcc ctgcggcatg
     1201 gcgaaacccg gcaccgttac cggctggaag ctgccgagcg acgagcgcga cgccctgctc
     1261 gagcgctttc cgccgcgcta cgccgaggtg atcgccgacc atgtgaccct gcgccttgga
     1321 gcgacccccg agacgccgct gccgcgcaag cccgacgccc ggatcatcgg ccgcgccgac
     1381 gacggcaaga gcctcgaatg cctggtggtc gagctcgacg ggaccaccga ccgccccgac
     1441 ggctcgacct accacatcac ctggtcgctg gggccgggcc gcaaggcgcg cgagagcaac
     1501 gacgtgctcc gtgacctggg ctgggagccg atcggcgccc cgatcccggt cgagctcgag
     1561 ccggcgcgct tttgagatgc cgggacctca accgcgcctg ttcctcgatg ccgacggagt
     1621 gctcgccgac ttcgattccg gagcccaggc gctgctcggg atgaccccgg ccgagttcga
     1681 ggcgcgccac ggccggcgcg agttctggcg gcggatcgcg accgcccgca atttctacgg
     1741 gactctgccc gagatgcccg acgcgcggcg gctgttcgac gcggtcgagc atctgaagcc
     1801 gaccatcctc accggccttc cgctcggcaa ttgggcggcc ccgcagaagg tcagatgggc
     1861 ggccgagcat ttccccggag tgccgatcat cacctgcatg gcgcgcgaca agcacaagca
     1921 catgaagccc ggcgacgtgc tggtcgacga ccgcgaaaac caccgcgcgt cctacgagcg
     1981 cgagggcgtc gtcttcgtcc atcacaaaag cgccgaggac agcctcaggc agttggcgca
     2041 gattttcccg tcggtgaagg ccgcggctta agcggccgcg atgacgtcga ccttctcgac
     2101 ccactcgggc cagaacaccg gctcgcggtt cgaccagccc ggcgcggtcg ccgcgctctc
     2161 gctgatcgac tgcagcagga cccgccggcg gtcggggtgg agatgcggaa gcgccgcggc
     2221 cgagcagaag gccgagggca gccacggccg cgccgaagcg ccgatcaggc gctcgtagag
     2281 gaagcggtag gacgcgaagt gcggcagccg gtcctggccg aggtcgaacg cggaaacggt
     2341 gatcagcatg ccgatgaacg gctcgatcat gtcgaggccg ctgtcgtcgg gaacctggag
     2401 gcgcatgtgg tcgagctgct tgtagagccg gccctgggcg cggatcgaag cggcctcgcc
     2461 ctcgtcgcgg gcccagcact cgatcgggtc gcggcggccg taggccacgt cgagcgagcg
     2521 gcgaatgtac cgctgcgtcg ccttcgagaa gctcgcgaac tccttcattt cggagatcag
     2581 catcgccccg ctcgccggct gactcgtccg cgtaatcatg aacccgactc ctttcgccta
     2641 ggcgggatca tgcggcggtt atagttgcgt tttgcttaac cacggtccca ccccccgttc
     2701 agattgaatc agaggggagt ccccgcggca acatgaaatc ttcactcgat gtcgcttaac
     2761 gccgccactg ttgattattt gcaacgcgtg gggtttttgt ttcgttggtg taacttattc
     2821 tggaaacccg gacgacggga ctgggtcgtc attctgtccg ggctgggccg cggccggcgg
     2881 gtccgaggcg tccatggact cgtcgctgcc gacatcggcc ttcttgtcga ggtcgtgcgg
     2941 attgtcgcgc agctcctttt cgagctcgcg ctccgggtcg tccgaaagca ccggcggcgg
     3001 cacgtcgctc ttctgctcgc cattcatcat cgtcactcct gtctctcgca cggctcaacg
     3061 agcggcgcgc caagcccgtt ccggagcgcg cctcctttcg acggaggagc ggcccgcttc
     3121 tgccaaccgg ccgacggccg attgctttcg agacccgcgg tggccagata gatttggatg
     3181 aacaaccatt cagaaatggc ggccgcgcga gcggattcac gcgccggtga cggcgggcgc
     3241 ttctccgact ggccgctggc gctgaagtcg atcctcggct tctggctgtt ctacgcgctg
     3301 accgtggtcg cccgggcgct gctcggggcc gaccccgcga cggtccttca gaacaagtcg
     3361 gtaacgatcg tcgcggggat tgtcctgacc gccgccatct atgccgccat ccagctgttc
     3421 gcggcccgcg ccggcctccg ccgccgagcg atcgtcgcgg tcgcggcctc gttcatcgcc
     3481 gccgggggcc aggccgcggt cctgatcgcg gccgaccgct accaggaaaa gccgcaggac
     3541 gagcacctct acgtatcgcg cgaggggtat cgcgtggtcg agctcggcaa tgccatgcgg
     3601 atcgagcgcg gcggccatcc gccgctgacc gtcacctggc ccaatatctc gtcgctcgac
     3661 ccctatctcc agttccggat cgcggcggac tcggcggtgg tgtggctgtt cttcttcgcc
     3721 gcttggagcg ccttctacct cgccaccctc gcccaggccg aggcgctcgg cgcccggcgc
     3781 cgcgtcgccg aagcggaaag cgcggcccgg gcggcgcagg tccgggcgct gcgctaccag
     3841 gtcaatccgc atttcctgtt caacacgctc aacagcctgt cctcgctggt catgaccggc
     3901 cgaaccgacc gcgcggaagc gatgctgctc
//
LOCUS       DASTZO010000241         2258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_724660,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000241 DASTZO010000000
VERSION     DASTZO010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724660"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1220
                     /locus_tag="VFU20_06685"
     CDS             <1..1220
                     /locus_tag="VFU20_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5286179.1"
                     /translation="RLAGTEAEARARTWAVARLKSLGFSDVRVETFDMPVWVRGEEKA
                     WITAPFPQPLVVTALGNSGATPPEGLEAELVGFDTLEALEAAPDEAVRGRIVFVSHAM
                     PRTQDGSGYGAFIGPRSRGPTVASRKGAAAIVVRSIGTDHHRNPHTGLQSFADGVAPI
                     PAGALSIPDAEQVQRILKRGKPVRIRLVMTPRAIGARQSGNVIAEAPGRDRAAAPLLV
                     ACHLDSWDLGTGAVDDAAGCGIVAAAAKRIMDSGRPLRTIRIVWFGAEEVGLFGGNDY
                     RARHEKEPYYAIAESDFGAGRVWKVDSKLGKAREAEARALQRALAPLGIVPGSLEQAG
                     GSDIGPLLADGAPGVTLNQDGTYYFDLHHTPDDTLDKIDLGDLRQNVAAWTTMLAVLS
                     GGIEPHPKRRDRR"
     gene            1245..1505
                     /locus_tag="VFU20_06690"
     CDS             1245..1505
                     /locus_tag="VFU20_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286180.1"
                     /translation="MRAAIILPALLLGACQVTKDDANDSVTVAYNQDVAENAAADVAN
                     TAENIGEDIANDVERTADKVQNEVGDVDVDVDVTRNAPSNSN"
     gene            complement(1555..1631)
                     /locus_tag="VFU20_06695"
     tRNA            complement(1555..1631)
                     /locus_tag="VFU20_06695"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1595..1597),aa:Pro,seq:tgg)
     gene            complement(1793..1864)
                     /locus_tag="VFU20_06700"
     tRNA            complement(1793..1864)
                     /locus_tag="VFU20_06700"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1830..1832),aa:Ile,seq:cat)
     gene            complement(1929..>2258)
                     /locus_tag="VFU20_06705"
     CDS             complement(1929..>2258)
                     /locus_tag="VFU20_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286181.1"
                     /translation="DSAPRLQLSTETRRRRCALEPALAERLVTRFCTALLGAAADGET
                     VVVHLDNASGRCLLWVRPPPGLDAAANGDPASGGFSLRLVRGLARIAGGDLTITKARI
                     TLALPEL"
BASE COUNT          373 a          803 c          784 g          298 t
ORIGIN      
        1 cgcggctcgc gggcaccgag gccgaggccc gggcacggac ctgggcggtg gccaggctca
       61 agtcgctggg gttttccgac gtccgcgtcg agacgtttga catgccggtg tgggtgcgcg
      121 gcgaggagaa agcgtggatc accgcgccct tcccgcagcc gctggtggtg accgcgctcg
      181 gcaacagcgg agcgaccccg ccggagggcc tcgaggccga gctggtcgga ttcgacaccc
      241 tcgaggcgct cgaggcggct ccggacgagg cggtccgcgg caggatcgtc ttcgtctccc
      301 acgcgatgcc gcgcacccag gacggctcgg gctacggagc gttcatcggc cccaggagcc
      361 gcggcccgac ggtcgccagc cgcaagggcg cggcggcgat cgtcgtccgc tcgatcggca
      421 ccgaccatca ccgcaatccc cacaccgggt tgcagagctt cgccgatggg gtcgccccga
      481 tcccggccgg ggcgctgtcg attcccgacg ccgagcaggt ccagcggatc ctcaagcgcg
      541 gcaagccggt gcggattcgg ctggtgatga ccccgcgcgc catcggcgcc cgccagtcgg
      601 gcaatgtgat cgccgaggca cccggccgcg accgcgccgc ggcgccgctg cttgtcgcct
      661 gccatctcga cagctgggac ctcggcaccg gcgcggtcga cgatgcggcg ggctgcggca
      721 tcgtcgccgc cgcagcgaag cggatcatgg actccgggcg gccgttgcgg accatccgca
      781 tcgtctggtt cggggccgag gaggtcgggc tgttcggcgg caacgactat cgggcccggc
      841 acgaaaagga gccttattat gcgatcgccg agagcgattt cggcgccggg cgggtgtgga
      901 aggtcgacag caagctcggc aaggcgcgcg aggccgaagc ccgggcgctg cagcgggcgc
      961 tcgcgccgct cggcatcgtc ccgggctcgc tcgagcaagc gggcggctcg gacatcggcc
     1021 cgttgctcgc cgacggagcg ccgggggtta ccctcaacca ggacggcact tattatttcg
     1081 acctgcacca cacgcccgac gatacgctcg acaagatcga cctcggcgac ctcaggcaaa
     1141 acgtcgcggc gtggacgacg atgctcgccg tgctatcggg agggatcgag ccgcatccca
     1201 agcgccgcga ccggcgttga tccggccgac caaggagaaa gccaatgcgt gctgcaatca
     1261 tcctccccgc cctgctgctc ggcgcctgcc aggtgaccaa ggacgacgcc aacgattcgg
     1321 tcaccgtcgc ctataaccag gacgtcgccg agaacgccgc cgccgacgtc gccaacaccg
     1381 ccgagaacat cggcgaggac atcgccaacg acgtcgagcg caccgccgac aaggtccaaa
     1441 acgaggtcgg cgacgtcgat gtcgacgtcg acgtcacccg caacgcgcct tcaaattcca
     1501 attaggaagg ccagctcgaa ccaggatcgc gccgccggcc ggcgtcggga cggatggtcg
     1561 gggagacagg attcgaacct gcgaccctct gctcccaaag cagatgcgct accaggctgc
     1621 gccactcccc gagcttcggg ccagttagca gcgcttgggt gagccgtcac gggagtaaat
     1681 ccccgggacg gcaatcgggc caaagccgca ctttggaccg cggcaagatg agatacgata
     1741 gggcgccctg ccccttcctg gatcaaagta ccaccttgat cgaaagcgcc ggtgggcccg
     1801 gagggaatcg aacccccaac caagccgtta tgagcggccg gctctaccgt tgagctacag
     1861 gccctgcccg gcccgcaagc gattagcggc gcccagccct tgcacgcaag tcatcgccac
     1921 cgcgcaagtc acagctcggg aagcgccagg gtgatgcgcg ccttggtgat cgtcaggtcg
     1981 ccgccggcga tccgcgccag gccgcgcacc agccgcaggc tgaagccgcc gctcgccggg
     2041 tcgccgttgg cggctgcgtc gaggcccggc ggcgggcgca cccacagcag gcagcggccg
     2101 ctcgcgttgt cgaggtggac gacgaccgtc tcgccgtccg cggccgctcc gagcagcgcc
     2161 gtgcagaagc gcgtcaccag ccgctcggcg agcgccggct cgagcgcgca gcggcgcctc
     2221 cttgtctcgg tcgacagctg gagccgcggg gcgctgtc
//
LOCUS       DASTZO010000242         3909 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_724791,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000242 DASTZO010000000
VERSION     DASTZO010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3909)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3909)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3909
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_724791"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VFU20_06710"
     CDS             complement(<1..592)
                     /locus_tag="VFU20_06710"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HEU5286182.1"
                     /translation="MGLGPRIDIRQSQSLVLTPQLTQAIKLLQLSNLELEAFIAEEMA
                     KNPLIEAIGGEEEGGGEEGGAPVAAPAMADAQAAGDEAPPDPGADELIAALGASDDQP
                     LDIDWREQALETDSFADVGASGGGSDEGFDFDRLEVGEASLSEHLLAQLHGLPGRDGE
                     LARAIAELLDETGYLSVPLSEVAEGHGVSRADAERAL"
     gene            complement(602..1375)
                     /gene="lptB"
                     /locus_tag="VFU20_06715"
     CDS             complement(602..1375)
                     /gene="lptB"
                     /locus_tag="VFU20_06715"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEU5286183.1"
                     /translation="MNEAATFARTAAGAALADGGLDVRSIAKAYDRRAVLHDVSLAVS
                     RGEVVGLLGPNGAGKTTCFYSIMGLVKPDSGRIFLDGQDITELPMYRRAILGIGYLPQ
                     ETSIVRGLTVEQNILAVLEVSEPDRAARMARLEKLLGEFGLTDLRDSPALALSGGERR
                     RCEIARSLAADPSIMLLDEPFAGIDPISISDIRDLVRELKQRGIGVLITDHNVRETLD
                     IVDRACIIYDGQVLFQGTPQALVADQEVRRLYLGESFAL"
     gene            complement(1384..1914)
                     /locus_tag="VFU20_06720"
     CDS             complement(1384..1914)
                     /locus_tag="VFU20_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HEU5286184.1"
                     /translation="MMAMRRHALLFAAAAALAGAAGLAQSPVSALKGHDSNAPIDVAA
                     DRIEVQDRADRAIFAGNVRVRQASLSLDTERLTVAYSSGGGIQIRRLDAAGGVVVRSP
                     SETARGNFGIYDLDKRLITLVGAVQLSRAGSRIMGSRLVIDLDSGRAVIDGGAPGVGQ
                     SGGRVTGHFTVPQRDR"
     gene            complement(1911..2546)
                     /locus_tag="VFU20_06725"
     CDS             complement(1911..2546)
                     /locus_tag="VFU20_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEU5286185.1"
                     /translation="MSEAATRERAFRQHWAVPGSSHDRLVRFAKVALPSAVGILIAVL
                     MFAPLDKEGDASFILDKNQVDNAPERMRVEAARYVGEDNRGQKFSIEAASAIQRSSDE
                     PIVDIRGMMARLGLAQGPLVILANAARYDLDAQKVAVNGPVRVVGPDGYRLETRDVEV
                     DLKQRRLASSGPVSGAMRLGQFRAAGLSADLGERKVVLHQARLKIVQGAVR"
     gene            complement(2571..3194)
                     /locus_tag="VFU20_06730"
     CDS             complement(2571..3194)
                     /locus_tag="VFU20_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEU5286186.1"
                     /translation="MTIHLHEEDLPAGVAFADGPIAVDTEAMGLVPGRDRLCLVQLSD
                     GKGDEHLVRFARGSDYSAPNLKALLGDPDRLKLYHFARFDIAAMRAYLGIVAAPVYCT
                     RTASRLVRTYTDRHGLKDLVKELLCIEISKQQQTSDWGADELSDAQREYAASDVRFLH
                     QLKEKLDERLVRENRVELAQACFDFLPHRALLDLAGWAEQDVFAHDG"
     gene            3427..>3909
                     /locus_tag="VFU20_06735"
     CDS             3427..>3909
                     /locus_tag="VFU20_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEU5286187.1"
                     /translation="MGFDKGRKGDRGGRGRDKRDDIFAEESSGDRGGFADRGFGGGPP
                     ARSYGGGGGGGYGDRGGGGGGGFRGGGGGGGGGGFRSGGGGAGGGMPPQVVGEGKGVV
                     KFFNPQKGFGFIVRDDGGEDVFVHISAVEQAGLTDLADGQPLEFTLVDRGGRISATNL
                     R"
BASE COUNT          561 a         1344 c         1390 g          614 t
ORIGIN      
        1 ccagggcacg ctccgcgtcc gctcgcgaca cgccgtggcc ttcggcgact tctgaaagag
       61 gaacgctgag atagccggtc tcgtcgagca gctcggcgat cgcccgcgcc agctcgccgt
      121 cgcggccggg cagcccgtgc agctgggcaa gcagatgttc gctcagcgac gcctcgccga
      181 cctcgagccg gtcgaagtcg aacccttcgt cgcttcctcc gcccgaagcg ccgacgtcgg
      241 cgaagctgtc ggtctcgagc gcctgctcgc gccagtcaat gtcgagcggc tggtcgtcgc
      301 tcgcgcccag cgccgcgatc aattcgtccg cgcccggatc gggcggcgct tcatcgcccg
      361 ccgcctgggc atcggccatt gccggcgcgg cgacgggcgc cccgccttcc tccccgccgc
      421 cttcctcctc gccgccgatc gcctcgatca gcgggttctt ggccatttcc tcggcgataa
      481 aggcttcgag ctcgaggttg ctcagctgca gcagcttgat cgcctgggtc agctggggag
      541 tgaggaccag cgactgcgat tggcggatgt cgatgcgcgg gccgagcccc atggccgtgc
      601 cctacagcgc gaagctctcg ccgaggtaga gccggcggac ctcctggtcg gcgaccagcg
      661 cctgcggcgt gccctggaac agcacctggc cgtcgtagat gatgcaggcg cggtcgacga
      721 tgtcgagcgt ctcacggacg ttgtggtcgg tgatcagaac gccgattccg cgctgcttca
      781 attcgcgcac caggtcgcgg atgtcggaga tcgagatcgg gtcgatgccc gcgaacggct
      841 cgtcgaggag catgatcgac gggtccgcgg cgagcgaccg ggcgatctcg cagcgccgcc
      901 gctcgccgcc cgacagagcg agcgccgggg agtcgcgcag atcggtcagc ccaaactcgc
      961 cgagcagctt ttcaaggcgg gccatgcgcg cggcccggtc cggctccgag acctcgagca
     1021 cggcgaggat gttctgctcg acggtgaggc cgcggacgat cgaggtttcc tgcggcagat
     1081 agccgattcc gaggatcgcc cggcggtaca tcggcagctc ggtgatgtcc tgcccgtcga
     1141 ggaagatgcg gccgctgtcg ggcttcacca gccccatgat cgagtaaaag caggtcgtct
     1201 tcccggcgcc gttggggccg agcaggccga cgacctcgcc gcgcgacacg gccagcgaca
     1261 cgtcgtggag caccgcccgg cggtcgtagg ccttggcgat cgagcggacg tccagcccgc
     1321 catcggcaag cgccgccccg gccgccgttc gagcgaaggt tgccgcctcg ttcatcccgg
     1381 gcctcagcgg tcccgctgcg gaaccgtgaa gtggccggtc acccggccgc cgctctggcc
     1441 gaccccgggc gctccgccgt cgatcaccgc tcggccggaa tccaggtcga tgaccagccg
     1501 cgagcccatg atccgcgatc cggcgcggct gagctggacc gccccgacca gggtgatcag
     1561 gcgcttgtcg aggtcgtaga tcccgaaatt gccgcgcgcg gtctcggacg ggcttcggac
     1621 gacgaccccg ccggccgcgt cgaggcgccg gatctggatc ccgccgccgc tcgaatacgc
     1681 gaccgtcagc cgctcggtgt cgagcgacag cgatgcctgg cggacccgca cgttgccggc
     1741 gaagatcgcc cggtcggcgc ggtcctgaac ctcgatgcgg tcggcggcga cgtcgatcgg
     1801 ggcgttgctg tcgtgcccct tgagcgccga gaccggcgac tgggcgaggc cggcggcgcc
     1861 ggcgagggcg gccgccgcgg cgaacaggag ggcgtgccgg cgcatggcca tcatctgacc
     1921 gccccctgga cgattttcaa gcgagcctgg tggagcacca ccttgcgctc gccgaggtcg
     1981 gcgctgaggc ccgcggcccg gaactggccc agccgcatcg ctcccgagac cgggcccgag
     2041 ctcgccagcc gccgctgctt caggtcgacc tcgacgtcgc gggtctcgag ccggtagccg
     2101 tccggcccga ccacccggac cggcccgttg accgcgacct tctgggcgtc gaggtcgtag
     2161 cgggcggcgt tggcgaggat caccagcggc ccttgcgcga ggccgagccg ggccatcatc
     2221 ccgcggatgt cgacgatggg ctcgtccgag ctgcgctgga tcgcggacgc cgcctcgatc
     2281 gagaatttct gcccgcgatt gtcctcgccg acgtagcgcg ccgcctcgac ccgcatccgc
     2341 tcgggcgcat tgtcgacctg gttcttgtcg aggatgaagc tggcgtcgcc ttccttgtcg
     2401 agcggggcga acatcagcac ggcgatcagg atcccgaccg cgctcggcag ggcgaccttg
     2461 gcgaagcgca ccagccggtc gtggctgctg ccgggcaccg cccaatgctg gcggaacgcc
     2521 cgctcacggg tcgccgcctc agacatggtg ggcctcaggc atggtgcgtc tcacccgtca
     2581 tgcgcgaaca cgtcctgctc ggcccagccg gcgaggtcga gcagggcccg gtggggaagg
     2641 aagtcgaagc aggcctgggc gagctcgacc cggttctcgc gcaccagccg ctcgtcgagc
     2701 ttctccttca gctggtggag gaagcgaacg tcgctggccg catattcgcg ctgtgcgtcg
     2761 ctgagctcgt cggcgcccca gtcgctggtc tgctgctgct tggaaatctc gatgcacagc
     2821 aattccttga ccaggtcctt gagcccgtgg cggtcggtgt aggtgcgcac cagccgcgag
     2881 gcggtgcggg tgcaatagac cggagcggcg acgatcccga ggtaggcgcg catcgccgcg
     2941 atgtcgaagc gcgcgaaatg atacagcttg agccggtcgg ggtcgccgag cagagccttc
     3001 agattgggcg ccgaatagtc gcttccgcgg gcgaagcgga cgaggtgctc gtcgcccttg
     3061 ccgtcggaca attggaccag gcacagccgg tcgcggccgg gaacaaggcc catcgcctcg
     3121 gtgtcgacgg cgatcggccc gtcggcgaac gccactccgg ccggcagatc ctcctcgtgc
     3181 agatggatgg tcattcgatc gtcctcaacc catgctcccg gccggtgcgc gcgcaccctc
     3241 tccggcactc tagtcgctgt tgcgccgcgc ttgccacccc tagtcccatt gagtcactag
     3301 agaaggctct gtttttcctg taagggcgtg aacggtgcgt gagcaggacg cgccgcgacc
     3361 ggttgcgcct atgggcccag cgcgttggtt tttgattgtt acgggcacaa ggaagtttgg
     3421 acgggcatgg gtttcgataa agggcgtaaa ggcgaccggg gcggtcgtgg gcgagataag
     3481 cgcgacgaca ttttcgcaga agagagttca ggtgaccgcg gcggcttcgc cgatcgcggc
     3541 ttcggcggcg gtcctcccgc ccgcagctac ggcggcggcg gtggcggcgg ctacggagat
     3601 cgcggcggcg gcggcggcgg tggattccgc ggcggcggcg gcggtggtgg cggcggtgga
     3661 ttccgcagcg gcggcggcgg cgcgggcggc ggaatgcctc cccaggtcgt cggcgaaggc
     3721 aagggcgtgg tcaaattctt caacccgcaa aaagggttcg gcttcatcgt ccgcgatgac
     3781 ggcggcgagg acgtgttcgt tcacatctcg gcggtcgagc aggcgggcct caccgacctc
     3841 gcggacggcc agccgctcga gttcacgctc gtcgaccgcg gcggccggat ctcggcgacc
     3901 aaccttcga
//
LOCUS       DASTZO010000243         4539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_774429,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000243 DASTZO010000000
VERSION     DASTZO010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_774429"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..178
                     /locus_tag="VFU20_06740"
     CDS             <1..178
                     /locus_tag="VFU20_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5286188.1"
                     /translation="IVSKALLRDAPEGPFSTNVLWDRAIAEGRCYGAVHQGLWFDVGN
                     PQSIRATEQMLANV"
     gene            171..3068
                     /locus_tag="VFU20_06745"
     CDS             171..3068
                     /locus_tag="VFU20_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEU5286189.1"
                     /translation="MSDAAAERPRAPAVFSIPAHRSFADSLVAGLRMRFGSGPLDLAR
                     GRILLPNNRAVRTVTEAFVRASGSGLVLPRLVPIGDPELEERIGGALDPADASDPVPP
                     AVDPLARLLVLTQLVAGDGTGTGEAARLAADLARVLDTLLIEDVDPARLADAAADAPE
                     LAGHWQLSLDRLRAILERRPARLAASGRIDLADRRNRLLRAMADRWRRAPPDGFTVAA
                     GITTAAPAVAVLLRCVADLPEGMVVLPGLSLVSVMPDEEWESLGPDEESRGEESHPQF
                     HLKLLLDRMGIARGEVRLWRASGRSAARAIRGRAVANAMTAADFSDKWNGLKPAERRL
                     SGVRAAELPDPAAEAQAIALALREAIETPGRTAALVTPDRMLAARVSALLERWEIVAD
                     DSAGKPLSQVAAGTLLLGIAAAAAEDLAPVPLLALLKHPLVGGEGEKRLAWLDGVRAL
                     DLALRGPRPAAGLAGLDEHFGPHAAWKKVRGLVAPLDGMLGAPIALATFAERLARSAD
                     SLAGDAAWRGQEGRMAAELLAELQALPEAAELSVSFEDAVAMLRQLLDERRVRPPYGG
                     HPRVFIWGLLEARLQQADLMVLGGLNEGVWPALPAPDPWLAPKIRANLRMPGLDFRTG
                     LAAHDFASALGAPSVLVTRARRDGRSPTVASRFWLRLRAMTGGLARDTRLERLAESLD
                     DPGVLEPAGRPRPSPPMEQRPRRISVTAVDRLKADPFAFYAQAMLGLRSLDSVDADHS
                     AAWKGTAVHKVLEQWLAQDECDPAKLLPRARALLEGAAIHPMLRALWQPRLIEAIRWV
                     EQQERDNRAAGRRPLKAEIWGEALIDGVTVHGTADRIDGLDGGGLAIIDYKTGKAPAQ
                     KAVDAGFALQVGLLGLIARAGGFEGARGEPAAHEYWSLTKYGDDFGKRVCPDKKMGAA
                     DFLAHAESHFIGAARKWLTGEEPFTAKLNPAYAPYGDYDQLMRLEEWYGRE"
     gene            complement(3071..3235)
                     /locus_tag="VFU20_06750"
     CDS             complement(3071..3235)
                     /locus_tag="VFU20_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286190.1"
                     /translation="MSDDDNKKDADDASEQRRAKLVKAGVAVGIGSAAIVAALLYASK
                     VKKTPDKSGK"
     gene            3283..>4539
                     /locus_tag="VFU20_06755"
     CDS             3283..>4539
                     /locus_tag="VFU20_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU5286191.1"
                     /translation="MRTRLKPFPELQGDQARASDPNVHAALSASAGTGKTQVLSARVL
                     RLLLSGVRPEHILCLTFTKAGAAEMANRINARLASWVRMRDQDLRSDLINLGEANDPP
                     TMQRARRLFARILDAPGGLRIQTIHSFAQALLAAFPAEVGIAPGFQPIEGRAEQELAR
                     ATLANLLADAESLGNEALIADVQCLSLRLGEQDAVAYLMQCARAGDALAALGAPDTIE
                     SKLRAAMRLPEGALEDYLADHCADDRFDCDLLQAIAAANRKWGSATGAGVADSVERWL
                     ALDPAARSAALPDLALLVFTGAGDLRKVQAGQRKADPDYDSHAERLAAAVTELLHIQN
                     GARLASDMAAGLRAGQAFAAAYSRAKRSAGVADFDDLIRWTRRLLETPGMGEWVRYKL
                     DRRTDHILVDESQDTNRDQWKIIKPLA"
BASE COUNT          615 a         1628 c         1660 g          636 t
ORIGIN      
        1 gatcgtctcc aaggcgctgc tcagggacgc gcccgagggg ccgttctcga ccaatgtgct
       61 gtgggaccgg gcgatcgccg aggggcgctg ctacggcgcc gtccaccagg gcctgtggtt
      121 cgacgtcggc aacccgcagt cgatccgggc gaccgagcaa atgctcgcca atgtctgacg
      181 ccgccgccga gcggccgagg gccccggcgg tcttcagcat cccggcgcac cgctcgttcg
      241 ccgattcgct ggtcgcggga ttgcggatgc ggttcggcag cggcccgctc gatcttgcgc
      301 gcgggcgaat cctgctgccc aacaaccgcg ccgtccggac ggtcaccgaa gccttcgttc
      361 gcgccagcgg ctccgggctg gtgttgccgc gcctggtgcc gatcggcgac cccgagctgg
      421 aagagcgaat cggcggggcg ctcgacccgg cggacgcgtc cgacccggtg ccgccggcgg
      481 tcgacccgct ggcgcgcctg ctggtcttga cccagctggt cgcgggcgac ggcacgggca
      541 ccggcgaagc ggcgcgcctg gccgcggacc tggcgcgggt gctcgatacg ctgctgatcg
      601 aagatgtcga tccggcgcga ctggccgatg cggccgccga tgcgcccgag cttgccggcc
      661 actggcagct gtcgctcgac cggctccggg cgatcctcga gcgcaggccg gctcggcttg
      721 cggcgagcgg ccggatcgac ctcgccgatc gccgcaaccg gttgctccgg gcgatggccg
      781 accggtggcg gagggcgccg cccgacggct tcaccgtcgc cgccggaatc accactgcgg
      841 cgcccgcggt ggcggtcctg ctgcgttgcg tcgccgatct gcccgagggg atggtcgtgc
      901 tgccggggct cagcctcgtc tcggtgatgc ccgacgagga gtgggagtcc ctcgggcccg
      961 acgaggagag caggggcgag gagagccatc cccagttcca cctgaagctg ctgctagacc
     1021 ggatggggat cgcgcgcggc gaggttcggc tgtggcgcgc ttcgggacgc tcggcggccc
     1081 gggcgatccg cgggcgcgcg gtcgccaatg cgatgaccgc cgccgacttc agcgacaagt
     1141 ggaacggcct caagcccgcc gagcggcgcc tgagcggggt tcgcgccgcc gagctgcccg
     1201 atccggccgc cgaggcccag gcgatcgcgc ttgcgctgcg cgaggcaatc gagactccgg
     1261 gccggaccgc cgcgctggtc actcccgacc ggatgctggc cgcgcgcgtc tcggcgctgc
     1321 tcgagcggtg ggagatcgtc gccgacgaca gcgccggcaa gccgctgtcg caagtcgccg
     1381 ccgggaccct gctgctcggg atcgccgccg ccgcggccga ggacctggcg ccggtgccgc
     1441 tgctggcgct gctcaagcat ccgctggtcg gcggcgaagg cgagaagcgg ctcgcgtggc
     1501 tggacggcgt ccgcgcgctc gacctcgcgc tgcgcgggcc gcggccggcg gcgggccttg
     1561 ccgggctcga cgagcatttc ggtccccacg cggcgtggaa aaaggtgcgc ggcctggtcg
     1621 ctccgctcga cgggatgctg ggcgccccga tcgctctggc cacgttcgcg gagcggctgg
     1681 cgcggtcggc ggactcgctt gccggcgacg ccgcctggcg agggcaggag ggccgcatgg
     1741 ccgcggagtt gctcgccgag ctccaggcgc tgcccgaggc cgcggagctt tccgtgagct
     1801 tcgaggacgc ggtcgcgatg ctccgccagc tgctcgacga gcggcgggtc cggccaccct
     1861 acggcggcca cccgcgggtg ttcatctggg ggctgctcga ggcgcgcctg cagcaggccg
     1921 atttgatggt gctcggcggg ttgaacgaag gcgtctggcc ggcgcttccg gcgcccgacc
     1981 cgtggctggc gccgaaaatc cgcgccaatc tccgaatgcc ggggctcgat ttccggaccg
     2041 gcctcgccgc gcacgatttc gccagcgcgc tcggagctcc gagcgtgctg gtcacccggg
     2101 cgcgccgcga cggccgctcc ccgaccgtcg cctcgcgctt ctggctgcgg cttcgggcga
     2161 tgaccggcgg cctcgcccgc gacaccaggc tcgaacggct cgccgagtcg ctcgacgacc
     2221 cgggcgtgct cgagccggcg ggccgcccgc ggccgtcgcc gcccatggag cagcggccgc
     2281 gaaggatctc ggtgaccgcg gtcgaccggc tgaaggcgga cccgttcgct ttctatgccc
     2341 aggcgatgct cgggctgcgg agcctcgatt cggtcgacgc cgaccacagc gccgcgtgga
     2401 agggcaccgc ggtgcacaag gtgctcgagc aatggctggc ccaagacgag tgcgatcccg
     2461 ccaagctcct gccgcgcgcc cgggcgctgc tcgaaggcgc ggcgatccac ccgatgctgc
     2521 gcgccttgtg gcagccgcgg ctgatcgagg cgatccgctg ggtcgagcag caggagcggg
     2581 acaaccgagc cgcggggagg cggcccctga aggccgagat ctggggggag gcactgatcg
     2641 acggcgtgac cgtccacggg accgcggacc ggatcgacgg gctcgacggc ggcggcctgg
     2701 cgatcatcga ctacaagacc ggcaaggcgc ccgcccagaa ggccgtcgac gccgggttcg
     2761 cgctccaggt cgggctgctc gggctgatcg cccgcgccgg cgggttcgag ggcgcgcggg
     2821 gcgagccggc cgcgcacgaa tattggtcgc tgaccaaata tggcgacgat ttcggcaagc
     2881 gcgtctgccc ggacaagaaa atgggcgccg ccgacttcct cgcccatgcc gaaagccatt
     2941 tcatcggcgc ggcgcgcaaa tggctgaccg gcgaggagcc gttcaccgcc aaattgaacc
     3001 cggcctacgc tccgtacggc gactatgacc agctgatgcg gctcgaggaa tggtacggcc
     3061 gcgaatgagc ctatttgccg gacttgtcgg gagtcttctt gaccttcgac gcgtagagca
     3121 gggcggcgac gatcgccgcc gagccgatgc cgaccgcgac cccggccttg acgagtttcg
     3181 cgcgccgctg ctcgctggcg tcgtccgcgt ccttcttgtt gtcgtcgtcg ctcatcgggg
     3241 gctttctatg gcctcgcccg ccgcttggga agcgggccgt tgatgcgcac ccgactcaag
     3301 cccttccccg agcttcaggg cgaccaggcg cgggcgtcgg acccgaacgt ccacgcggcg
     3361 ctgtcggcgt cggccggcac cggcaagacc caggtgctga gcgcgcgcgt tttgcggctg
     3421 ctcctgtcgg gagtccggcc cgagcatatc ctctgcctga ccttcaccaa ggccggcgcc
     3481 gcggagatgg ccaaccgaat caatgcgcgc ctcgcgagct gggtccggat gcgcgaccag
     3541 gatttgagaa gcgatctcat caacctcggg gaagccaatg atccgccgac catgcagcgg
     3601 gcgcggcggc tattcgccag gatcctcgac gcgcccggcg gattgcgcat ccagaccatc
     3661 cacagcttcg cccaggccct gctcgccgca ttcccggccg aggtggggat cgcgcccggc
     3721 ttccagccga tcgagggccg cgccgagcag gagctggcgc gggcgacgct ggcgaacctg
     3781 ctcgccgatg ccgaaagcct gggcaacgaa gcgctgatcg ccgacgtcca gtgcctcagc
     3841 ctgcgcctcg gcgaacagga cgcggtcgct tatctgatgc aatgcgcgcg ggcgggcgac
     3901 gcgctggcgg ccctgggcgc gcccgacacc atcgagtcca agctgcgggc ggcgatgcgg
     3961 ctgcccgaag gcgcgctcga ggattatctc gccgaccatt gcgccgacga ccgcttcgat
     4021 tgcgacctgt tgcaggcgat cgccgcggcc aaccgcaaat ggggcagcgc caccggcgcc
     4081 ggcgtcgccg acagcgtcga gcgctggctg gcactcgacc cggcggcgcg ctcggccgcg
     4141 cttcccgacc tggccttgct ggtgttcacc ggcgccggcg acctgcgcaa ggtccaggcg
     4201 ggccagcgca aggccgatcc ggactatgat tcccacgcgg agcgactggc cgcggcggtg
     4261 accgagctgc tccacatcca gaacggcgcc cgcctcgcat ccgacatggc ggcgggcctt
     4321 cgtgccgggc aggcgttcgc cgccgcctac agccgcgcca agcgcagcgc cggggtggcc
     4381 gatttcgacg atctgatccg ctggacccgg cggctgctcg agactcccgg catgggcgag
     4441 tgggtgcgct acaagctcga ccggcgcacc gaccatatcc tggtcgacga atcccaggac
     4501 accaaccgcg accagtggaa aatcatcaag ccgctggcc
//
LOCUS       DASTZO010000244         9246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1143579,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000244 DASTZO010000000
VERSION     DASTZO010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 9246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9246
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143579"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..1145
                     /locus_tag="VFU20_06760"
     CDS             9..1145
                     /locus_tag="VFU20_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5286192.1"
                     /translation="MVDRSNRRRNIIIAALIVVAALAAAWFLFGKGDDPAATAGAGQG
                     GRRGQIPTVSIIVPGQSQVGRTLTASGALAARRDQPVGVAGEGGLVRSVTVDAGSWVR
                     QGQVLASVDRSVQTQTAAQLGASIQVARADAALAQNELDRSQSLVGRGFVSKADLDRK
                     RAARDAAAARVRVAQAQFGATRARVGRLDIRAPTGGLVLARNVEVGQVVGPGAGALFR
                     LAAGGEMEMRAQIPQQDIAFVRAGMPASVTPIGLDRSFSGSVWQVSPVIDPQSRQGEV
                     RIAIPYDPAIRPGGFAEARIGAGGTTAPLLPQSAVLSDEKGNYVYIVNSKNEVERRDV
                     KIGSVDDEGVTIIEGLTGQEAVVLSAGPFLNPGQKVSPKRQAAR"
     gene            1171..4248
                     /locus_tag="VFU20_06765"
     CDS             1171..4248
                     /locus_tag="VFU20_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5286193.1"
                     /translation="MNFRNISSWCIRNPVPPIVLFIGLMLAGLVSFANMQVNNNPDVD
                     FPAAEVTIAQPGAAPTEMESQITQRVESAIRGVEGVDEINSSIREGNSSTFVQFEIGT
                     PTDRAVNDIRDAISQIRGDLPDGILEPRINRVDIAGDPILFVAAETTDMSLEELSWYV
                     DNDVGKRLLGVEGVAAVSREGGVDRQIRVVLDPTALQAQGITAAQVNQQLRQTNLNAA
                     GGRAEIAGSEQSVRVLGNARDAFDLSQTQIVVPGGRTVRLADLGEVKDAYSEQRTIAK
                     MNGRQVISFNVQRSKGASEVTTYDAAWAELRKIEKENPRIRFLEIFNQVDYTKSQYHS
                     AMEGLWEGAILAVFVVFLFLRDMRATVISAFAIPLSAIPTFWFMSMLGITLNGLSLLA
                     MSLVAGVLVDDAIVEIENIVRHMRMGKSAYQASIDAADEIGLAVLATTMSIVAVFLPV
                     ALMPGISGQFFKSFGYTVVIAVMMSLLVARMITPLMAAYFLRSKGVQPHASGWAMFKY
                     LDILKWTLRHRWAAVVGGIASLVLTGIIFASLGMQFQPPLNLDFSRARVGMAPGATLD
                     QTEAKVQQAAEIVEKDPDVEHVFQRVFVGAGFLNIVLKKDREKTSTEFERGLTPALSA
                     IPDAQVNFLSQGGGGPGGAPRDIMLYLGGDNPELLLATANKIADEMQTLSELRAPRVQ
                     GDLVRPEITIKPRFDLAADLGVTTQALSQTIRIATLGDIAQNSAKFSLADRQVPITVS
                     LSENSRRDLATLENLPVPTTGGGSVPLKAVAEIGFGSGPTTVQRTNQIRRILVGADLA
                     PGLVASDAWTKINQLPTVKNLPQGVSRLSLGEQKWQAELLYYFAIALASGVLLVFAVL
                     VLLYRRFLAPFVNMGSLLLAPLGAGIGLLIAGQPVSLPVLIGVLMLFGIVAKNSILLV
                     DFAVEMMNHGMPKDEAIYEAGHKRAQPIVMTTVAMVAGMLPIALSLSGDSSWRAPMGV
                     TVIGGLIFSTILTLLLVPAYFSIAIDVEKWIGRKFHRLVAHDATRPPEPVPAE"
     gene            4280..5536
                     /locus_tag="VFU20_06770"
     CDS             4280..5536
                     /locus_tag="VFU20_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010122995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HEU5286194.1"
                     /translation="MTSPALARFNPSQPGAAGMKAAATGLLVAMAALFAITRAFEPAY
                     PWLGYVKAFAEAAMVGGLADWFAVTALFRHPLGLPIPHTAIIPRNKDRIGEALASFLK
                     ENFLIAAVVARRMRNIDIAGAAGRFLRAPEGEGTRIRHGASRLIADIFEGLDDERLGG
                     IVKGAISSRIRAMEVSPLLGHALASAINEDRHVPMLEAGIRWMARALDANESLIRDMV
                     HKRASWVLKLAGLDTKLADAIVDGLRKLTVEMSTDPAHPVRRKIEEALAQLANDLQTR
                     PETRERVEAMKEELLANRSVSLWLDTLWQKGREAIIRAARNPDAALAGKLGEVLKSMG
                     TTLEGDPRIRAAINQFARRAVAGLAASYGSSIVKLVSETIRSWDARTITARLEAAVGR
                     DLQYIRINGTLVGGLVGVILHALDGL"
     gene            5607..6929
                     /locus_tag="VFU20_06775"
     CDS             5607..6929
                     /locus_tag="VFU20_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5286195.1"
                     /translation="MRKLALVTAAACFATPAAAQTAAAPAEAVTVIHAGTLIAEPGRP
                     PLRNATIVVRGRQIAEVRPGFESLPGAQVIDLRGSTVLPGLIDMHVHLSGLDDRLQRR
                     LQQTERDYEDEAFTALLNARKTLFAGFTTVRDLGAEPRLITSLRDTLASGAFAGPTIV
                     AAGAGISGSGGHSDRRNGLNRQVASTVSANNLCNGPDDCRRAVREQVGLGADVIKITA
                     TGGVLSNVAGGLNKQMMDDEMRAVVETARLFGRKVAAHAHGVDGVNAALRAGVHSIEH
                     GTFTNEETFKLYRQTGAYYVPTLLAPASALADGVRGALTPAQYDKARQAAGNAEKSFA
                     RAIREGVKIAFGTDTGVSPHGRNAEEFALMIRAGMTPAAAIRSATVDAATLLGRETAI
                     GTIEAGKDADIIAVDGDPLADVRLLERVGFVMKHGRVYKQAGRAAVAD"
     gene            complement(6926..8209)
                     /gene="murA"
                     /locus_tag="VFU20_06780"
     CDS             complement(6926..8209)
                     /gene="murA"
                     /locus_tag="VFU20_06780"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007012691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU5286196.1"
                     /translation="MDSIWIQGGTRLEGKIPISGAKNAALTLLPCALLTEDRLTLGNL
                     PRLADVDSFGHLLNQLGVSTMVEGVRKGEFGRRMTLHANDIASTVAPYDMVRKMRASI
                     LVLGPMLARAGEATVSLPGGCAIGDRPIDLHLQALECLGAEIELAAGYVKATAPKGRL
                     PGGDYSFPVVSVGATENALMAAVLATGRSQLFNAAREPEIADLCNLLVAMGARIEGIG
                     SSHLIIEGVEALHGTSYDVMPDRIEAGSYACAAGITGGCIELVGARPGDMCAIINALS
                     HAGLIVEFNDHGIKVSADGKLRPLALTTAPFPGFPTDMQAQFMAMLSLAEGESFLEET
                     IFENRYMHVPELRRMGAEIEIRGRSALVHGVDKLTGAAVMATDLRASMSLVLAGLAAD
                     AETEVLRVYHLDRGYERLEEKLSAVGATIERRTSN"
     gene            complement(8380..8712)
                     /gene="clpS"
                     /locus_tag="VFU20_06785"
     CDS             complement(8380..8712)
                     /gene="clpS"
                     /locus_tag="VFU20_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HEU5286197.1"
                     /translation="MANEPGEGDGLEDIETGVATRTRPRTKKPSNYKVLMLNDDYTPM
                     EFVVLVLQRFFSMSIEDATRVMLQVHQQGVAVCGVFTYEVAETKVTQVIDFARENQHP
                     LQCTLEKA"
     gene            complement(8865..>9246)
                     /locus_tag="VFU20_06790"
     CDS             complement(8865..>9246)
                     /locus_tag="VFU20_06790"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEU5286198.1"
                     /translation="ERGQEAVKRSQKVAQELADIGRANVEAVVEATRVATEGARSIGQ
                     DAVARTREGAEQAADAIRSLAEAKSPTEFVQLQSELARTSFDRLVAESSQLTESMVKL
                     AGKAFQPLSNRATANAERFNNLVA"
BASE COUNT         1463 a         3203 c         3164 g         1416 t
ORIGIN      
        1 cgctggtcgt ggtcgatcgg tcgaatcgtc ggcgcaacat catcattgcg gccttgatcg
       61 tcgtcgcggc gcttgcggcg gcgtggttcc tgttcggcaa gggtgacgat cccgccgcga
      121 ccgccggcgc aggccagggc ggacgacgcg gccagattcc gaccgtgtcg atcatcgtgc
      181 cgggacaaag ccaggtcggc cgcacgctga ccgcgtcggg cgcgctggcc gcgcgtcgcg
      241 accagccggt cggtgtcgcg ggcgaaggcg gcctcgtccg ctccgtcacc gtcgatgccg
      301 gcagctgggt ccgccaaggc caagtgctcg ccagcgtcga tcgctcggta cagacacaga
      361 ccgccgccca actcggcgcg tcgattcagg tcgcgcgcgc cgacgccgcg cttgcccaga
      421 atgaactcga ccgcagccag tcgctggtcg gccgcggttt cgtgtcgaag gctgacctcg
      481 accgcaagcg cgccgcgcgc gacgccgccg cggcgcgcgt gcgggtcgcc caggcgcagt
      541 tcggagcaac tcgggcacgc gtcggacgcc tcgacatccg cgcgccgacc ggcggcctgg
      601 tgctcgcccg caacgtcgag gtcgggcagg tcgtcgggcc gggcgcgggc gccctgttcc
      661 ggctcgccgc cggcggcgag atggagatgc gcgcccagat cccgcagcag gacatcgcct
      721 tcgtccgcgc cgggatgccg gccagcgtca ccccgatcgg gctcgaccgc agcttctccg
      781 gctcggtgtg gcaggtgtcg ccggtgatcg acccgcagtc gcgccagggc gaggtgagga
      841 tcgccatccc ctacgatccg gcaatccgcc ccggcggctt cgcggaagcc cggatcggcg
      901 ccggcgggac gaccgccccg ctgcttccgc aaagcgcggt gctcagcgac gagaagggca
      961 attacgtcta catcgtcaac tcgaagaacg aggtcgagcg gcgcgacgtc aagatcggct
     1021 cggtcgacga cgaaggggtg acgatcatcg aagggctcac cggccaggag gcggtggtgc
     1081 tgtcggcggg gccattcctc aaccccgggc aaaaggtgtc gccgaagcgc caggccgcgc
     1141 gctgacgggc atgaaaagag ctagagcgac atgaacttcc gcaacatctc gtcctggtgc
     1201 atccgcaacc cggttccgcc gatcgtgctg ttcatcgggc tgatgctggc cggcctggtc
     1261 tcgttcgcga acatgcaggt caacaacaat ccggacgtcg atttcccggc cgcggaagtg
     1321 accatcgccc agcccggcgc cgcaccgacc gaaatggaaa gccagatcac gcagcgggtc
     1381 gaatcggcga tccgcggggt cgagggcgtc gacgagatca acagctcgat ccgcgaaggc
     1441 aacagcagca ctttcgtcca gttcgagatc ggcactccga ccgaccgggc ggtcaacgac
     1501 atccgcgacg cgatctcgca gatccgcggc gacctgcccg acggcatcct cgagccgcgg
     1561 atcaaccgcg tcgacatcgc cggcgacccg atcctgttcg tcgccgccga gaccaccgac
     1621 atgagcctgg aagagctcag ctggtacgtc gacaacgacg tcggcaagcg gctgctcggg
     1681 gtcgagggcg tcgcggccgt gtcgcgcgag ggcggcgtcg accgccagat ccgggtggtc
     1741 ctcgacccga ccgcgctcca ggcccagggg atcaccgccg cccaggtcaa ccagcagctt
     1801 cgccagacca acctcaacgc cgccggcggc cgcgccgaga tcgccggctc cgagcagtcg
     1861 gtgcgcgtgc tcggcaatgc ccgcgacgcc ttcgatttgt cgcagaccca gatcgtggtc
     1921 cccggcggcc gcacggtccg gctggccgac ctcggcgagg tcaaggacgc ttattccgag
     1981 cagcgcacca tcgccaagat gaacgggcgg caggtgatca gcttcaacgt ccagcgctcc
     2041 aagggcgcct cggaagtcac cacctacgac gccgcgtggg cggagctgcg caagatcgag
     2101 aaggaaaacc cgcggatccg gttcctcgag atcttcaacc aggtcgatta caccaagtcg
     2161 caatatcatt cggcgatgga aggcctgtgg gagggcgcga tcctcgccgt cttcgtcgtc
     2221 ttcctgttcc tccgcgacat gcgcgccacg gtcatctcgg cattcgcgat cccgctgtcg
     2281 gcgatcccga ccttctggtt catgagcatg ctcggcatca ccctgaacgg cctgtcgctg
     2341 ctggcgatga gcctggtcgc cggagtcctg gtcgacgacg cgatcgtcga gatcgagaac
     2401 atcgtccgcc acatgcggat gggcaaatcc gcctaccagg cgtcgatcga cgccgccgac
     2461 gagatcggcc tcgcggtgct tgcgaccacc atgtcgatcg tcgccgtgtt cctgccggtc
     2521 gcgctgatgc ctggcatctc gggccagttc ttcaagagct tcggctacac ggtcgtcatc
     2581 gccgtgatga tgagcctgct cgtcgcgcgc atgatcactc cgctgatggc ggcctatttc
     2641 ctgcgctcca aaggcgtcca gccgcacgcg tcgggctggg cgatgttcaa atatctcgac
     2701 atcctgaaat ggactttgcg gcaccgctgg gccgcggtgg tcggcgggat cgcgtcgctg
     2761 gttttgacgg ggatcatctt cgccagcctc gggatgcagt tccagccccc gctcaacctc
     2821 gatttctcgc gggcgcgggt cggaatggcg ccgggcgcga cgctcgacca gaccgaggcc
     2881 aaggtccagc aggcggcgga gatcgtcgag aaggaccccg acgtcgagca tgtcttccag
     2941 cgggtgttcg tcggcgccgg cttcctcaac attgtcctca agaaggaccg cgagaagacc
     3001 agcaccgagt tcgagcgcgg cctgacgccg gcgctgagcg cgattcccga cgcccaggtg
     3061 aatttcctga gccaaggcgg cgggggcccc ggcggcgccc cgcgcgacat catgctgtac
     3121 ctcggcggcg acaacccgga actgctgctg gcgaccgcca acaagatcgc cgacgagatg
     3181 cagaccctgt ccgaattgcg cgcgccgcgg gtccagggcg acctcgtccg gccggagatc
     3241 acgatcaagc cgcgcttcga cctcgccgcc gacctcgggg tcacgaccca ggcgctgagc
     3301 cagacgatcc gcatcgcgac gctcggagat atcgcccaga acagcgccaa attctcgctc
     3361 gccgaccgcc aggtgccaat caccgtgtcg ctgtccgaga acagccggcg cgatctggcg
     3421 acgctcgaga atctgccggt gccgactacc ggcggcggct cggtgccgtt gaaggcggtg
     3481 gccgagatcg gcttcgggtc ggggccgacg acggtccagc ggaccaacca gatccgccgg
     3541 atcctggtgg gagcggactt ggcgccggga ctggtcgcga gcgacgcctg gaccaagatc
     3601 aaccagctgc cgaccgtaaa gaacctgccg caaggggtat ccaggctcag cctcggcgag
     3661 cagaaatggc aggcggaatt gctctattat ttcgccatcg cgctggcgtc gggcgtgctg
     3721 ctggtgttcg ccgtgctggt gctgctctac cgccgcttcc tcgccccgtt cgtcaacatg
     3781 ggctcgcttc tgctcgcccc gctgggcgcg ggaatcggcc tgctcatcgc cggccagccg
     3841 gtgtcgctgc cggtcctgat cggcgttctg atgctgttcg gcatcgtcgc caagaactcg
     3901 atcctgctgg tcgacttcgc ggtcgagatg atgaaccacg gaatgcccaa ggacgaggcg
     3961 atctacgagg ccgggcacaa gcgcgcgcag ccgatcgtca tgacgacggt ggcgatggtc
     4021 gcgggcatgc tgccgatcgc gctgtcgctg tccggggatt cgagctggcg ggcgccgatg
     4081 ggcgtgacgg tgatcggcgg cctgatcttc tcgaccatcc tgacgctgct cctggtgccg
     4141 gcctatttct cgatcgcgat cgacgtcgag aaatggatcg ggcgcaagtt ccaccgactt
     4201 gtcgcgcacg acgcgacccg cccgccggag cccgtgccgg cggagtgatt tcgctcgatc
     4261 ggcccgctat ggcggcgcga tgacgtcccc ggcgctcgcc cgcttcaacc cttcgcagcc
     4321 gggcgcggcg gggatgaagg ccgccgccac cggcctgctg gtcgcgatgg cggccctgtt
     4381 cgcgatcacc cgggcattcg agcccgccta cccgtggctc ggctacgtca aggcgttcgc
     4441 cgaggcggcg atggtcggcg gcctcgccga ctggttcgcg gtcaccgctc tgttccgcca
     4501 cccgctcggg ctgccgatcc cgcacaccgc gatcatcccg cgcaacaagg accgcatcgg
     4561 cgaggcgctc gcctcgttcc tcaaggagaa tttcctgatc gccgcggtgg tcgcgcggcg
     4621 gatgcgcaac atcgacatcg ccggcgccgc cgggcgcttc ctgcgcgccc cggaaggcga
     4681 agggacccgc atccggcacg gcgcctcgcg gctcatcgcc gacattttcg aggggctcga
     4741 cgacgagcgg ctgggcggca tcgtcaaggg cgcgatctcg tcgcgaatcc gggcgatgga
     4801 ggtctcgccc ctgctcggcc atgcgctcgc ctcggcgatc aacgaggacc gccacgtgcc
     4861 gatgctcgag gccgggatcc gctggatggc ccgggcgctc gacgccaacg aaagcctgat
     4921 ccgcgatatg gtccacaagc gcgccagctg ggtgctcaag ctcgccgggc tcgacaccaa
     4981 gctcgccgac gcaatcgtcg acggcttgcg caagctgacg gtcgagatgt cgaccgaccc
     5041 cgcgcacccg gtgcgccgca agatcgagga ggccctcgcc cagctcgcca acgacctcca
     5101 gacccggccc gagacccgcg agcgggtcga ggcgatgaag gaagagctgc tcgccaaccg
     5161 gtcggtcagc ctgtggctcg acacgctgtg gcagaagggc cgcgaagcga tcatccgcgc
     5221 cgcccgcaac cccgacgcgg cgctggccgg caagctgggc gaggtgctga agtcgatggg
     5281 caccacgctc gagggcgacc cgcggatccg ggccgcgatc aaccagttcg cgcggcgcgc
     5341 ggtcgccggg ctggcggcga gctacggaag ctcgatcgtc aagctggtgt cggagactat
     5401 ccgcagctgg gacgcccgga cgattaccgc ccggctcgag gcggcggtcg ggcgcgacct
     5461 ccagtatatc cggatcaacg gcacgctggt cggcggcctg gtcggggtga tcctgcacgc
     5521 gctcgacggg ctgtgatcca cccttgcgtt ccgccgggcg agcggctttg ctggccagag
     5581 cccagccagg acgtcaggaa ttgcccgtga ggaaactcgc cctcgtcacc gccgccgcct
     5641 gcttcgccac gccggccgcc gcccagaccg cggctgcccc ggccgaagcg gtcaccgtca
     5701 tccacgccgg aacgctgatc gccgagccgg gccggccgcc gctgcgcaac gcgaccattg
     5761 tcgtccgcgg ccggcagatc gccgaagtgc gcccgggctt cgagtcgctg ccgggggcgc
     5821 aagtcatcga cctgcgcgga tcgacggtgc tgcccgggct gatcgacatg catgtgcacc
     5881 tgtcgggcct cgacgaccgc ctgcagcggc gcctccagca aaccgagcgc gactatgagg
     5941 acgaggcgtt cacagcgctt ctcaacgcgc gcaagacgct gttcgccgga ttcaccaccg
     6001 ttcgcgacct cggcgccgaa ccgcgcctga tcacctcgct ccgcgacacg ctcgccagcg
     6061 gcgccttcgc cggcccgacg atcgtggcgg cgggggccgg catttcgggc agcggcgggc
     6121 attcggaccg gcgcaacggc ctcaatcgcc aggtcgcatc caccgtctcg gcaaacaatc
     6181 tgtgcaacgg cccggacgac tgccgccggg cggtgcgcga gcaggtcggc ctgggcgccg
     6241 acgtgatcaa gatcaccgcg acgggcgggg tgctcagcaa cgtcgccggt gggctcaaca
     6301 agcagatgat ggatgacgag atgcgcgcgg tggtcgagac cgcgcgcctg ttcggccgca
     6361 aggtcgccgc ccatgcgcac ggagtcgacg gggtcaacgc cgcgcttcgc gccggagtcc
     6421 attcgatcga gcacggcacc ttcaccaacg aggagacctt caagctctat cgccagaccg
     6481 gcgcttatta cgtgccgacc ctgctcgcgc ccgcttcggc gctcgccgac ggagtgcgcg
     6541 gcgccctcac tccggcccag tacgacaagg cgcggcaggc ggcgggcaac gccgagaaga
     6601 gcttcgccag ggcgatccgc gaaggcgtca agatcgcctt cggcaccgac accggagtga
     6661 gcccgcacgg ccgcaacgcc gaggagttcg cgctgatgat ccgggccggg atgactccgg
     6721 cggcggcgat ccggtcggcg accgtcgatg ccgcgaccct gctcggccgc gagacggcga
     6781 tcggcaccat cgaggccggc aaggacgccg acatcatcgc cgtcgacggc gacccgctcg
     6841 ccgacgttcg gctgctcgag cgggtcggct tcgtcatgaa gcacggccgc gtctacaagc
     6901 aggccggccg ggcggcggtc gccgactagt tcgaggttcg ccgctcgatc gtcgctccga
     6961 ccgcggacag tttctcctcg agccgctcgt agccgcggtc gaggtgatag acccgcagca
     7021 cctcggtctc ggcatcggcg gcgaggccgg ccagcaccag gctcatcgaa gcccgcaggt
     7081 cggtcgccat gaccgccgca ccggtcagct tgtcgactcc gtgaaccagg gccgaacgcc
     7141 cgcggatctc gatctcggcg cccatccggc ggagctcggg gacgtgcatg tagcggttct
     7201 cgaagatcgt ctcctcgagg aagctctcgc cctcggccag gctgagcatc gccatgaact
     7261 gcgcctgcat gtcggtcggg aagcccggga acggagcggt ggtcagggcc agcggccgca
     7321 gcttgccgtc ggcgctgacc ttgatgccat ggtcgttgaa ctcgacgatc agcccggcgt
     7381 ggctgagcgc gttgatgatc gcgcacatgt cgccgggccg cgctccgacc agctcgatgc
     7441 agccaccggt gatgcccgcg gcgcaggcgt agctgccggc ttcgatccgg tcgggcatga
     7501 cgtcatagct ggtgccgtgg agggcctcga ccccctcgat gatcagatgg ctcgagccga
     7561 tgccctcgat cctggcgccc atcgccacga gcaggttgca caaatcggcg atctcgggct
     7621 cgcgggcggc gttgaacaat tgcgagcggc cggtcgcgag caccgccgcc atcagcgcgt
     7681 tctcggtcgc cccgaccgac accaccggga agctgtagtc gccgcccggc aggcggccct
     7741 ttggagcggt cgccttcacg tagccggcgg cgagctcgat ctcggcgccg aggcattcga
     7801 gcgcctgcag atggaggtcg atcgggcggt cgccgatcgc gcagccgccg ggcagcgaga
     7861 cggtggcttc gccggcgcgg gccagcatcg gccccaggac gaggatcgag gcgcgcatct
     7921 tgcggaccat gtcgtagggc gcgaccgtcg aggcgatatc gttcgcgtgg agagtcatcc
     7981 ggcggccgaa ctcgcccttc cggacgccct cgaccatggt cgagactccg agctggttga
     8041 gcagatgacc gaagctgtcg acgtcggcga gccgcggcag attgccgagg gtcagccggt
     8101 cctcggtcag gagcgcgcag ggcagcaggg tcaaagccgc gttcttggct ccggaaatgg
     8161 gaatcttccc ctcgaggcgc gtgccgccct ggatccagat gctgtccatt ggtggggttt
     8221 agcggacgcg ggtgggctgt aaatcctccc ctgcatttgc aggggagggg gaccggcgca
     8281 gccggtggag gggccttccc ggccgcataa gagcccctcc accatcgaat cttcgatcga
     8341 tggtccccct cccctcgaaa tcgaggggag gaaccaggct caggccttct ccagcgtgca
     8401 ctggagcggg tgctggttct cgcgggcgaa gtcgatcacc tgggtgacct tggtctcggc
     8461 gacctcgtag gtaaagacac cgcatactgc gacgccctgc tggtggacct ggagcatcac
     8521 ccgggtcgcg tcctcgatgc tcatcgagaa gaagcgctgc aggacgagga cgacgaactc
     8581 catcggagtg taatcgtcgt tgagcatcag caccttgtag ttggacggct tcttggtcct
     8641 ggggcgggtg cgggtggcga cgccggtctc gatatcctcg agcccgtcgc cctctcccgg
     8701 ctcattcgcc atcatcgtcc agcgcatcat catgtgagac cgtaaatatg gcaagcgctc
     8761 aaccctcggc aaggcgcaag cgggcggagc ggagacgaaa aaaaggcggc cgcccctggg
     8821 gaggagcggc cgccggcatt acctgacttt aggaggggag agggtcaggc aacgagattg
     8881 ttgaagcgct cggcattggc cgtggcgcgg ttcgacagcg gctggaacgc cttgccggcg
     8941 agcttgacca tcgattcggt cagctgcgag ctctcggcga ccaggcggtc gaacgaggtc
     9001 cgggcaagct cgctctggag ctgcacgaac tcggtcggcg acttggcctc ggcgagcgag
     9061 cggatcgcgt cggcggcctg ctcggcgcct tcgcgggtgc gggcaaccgc gtcctggccg
     9121 atcgagcggg cgccctcggt ggcgacgcga gtcgcctcga cgaccgcctc gacgttggcg
     9181 cggccgatgt cggccagctc ctgggcgacc ttctgcgagc gcttgaccgc ttcctggccg
     9241 cgctcg
//
LOCUS       DASTZO010000245         6927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1117756,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000245 DASTZO010000000
VERSION     DASTZO010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117756"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="VFU20_06795"
     CDS             <1..804
                     /locus_tag="VFU20_06795"
                     /inference="COORDINATES: protein motif:HMM:NF016497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEU5286199.1"
                     /translation="DALPEKPAWAQATQDFWAPSVICDGSTYFLYYSATPDVCHDPRR
                     GHCLAVATSSSPAGPFTDMGIPLLHGMGFEYIDPMAYDDPVTGKRLLYWGSGFQPIKV
                     QELSEDRLSFEPGSQPVDLVWPNPVKGAFPRLVEASWVIRHDDFYYLFYSGDNCCGPD
                     ADYGVMVARSASATGPFETLEQARGVPHSLMLFRSERWLAPGHNTVVTDKAGQAWIIY
                     HAIDVNRPRQRQEDEINSRRILMMDRIEWRDGWPYVGTPSDEPRAGPVV"
     gene            complement(835..2001)
                     /locus_tag="VFU20_06800"
     CDS             complement(835..2001)
                     /locus_tag="VFU20_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286200.1"
                     /translation="MNNHPGKTFASFFQAGFECSSHRRRDGVRLDLIRATSHDRHLES
                     DYRQCLELGFRTLRDGLRWHLIEQSPGRYDWSSWLPALEASERLGLQVIWDLFHYGSP
                     DHVDQAGPDFPQRFTDFAVAALEVQQSVSNRPPIVCPLNEINFLSWAVDDGYFPPVGP
                     GRRGWFKHHLLRTAIAAARAIREQWPETTIVWAEPLIHIAPHDRRRRTVRAAEQNLQG
                     MYEAYDSIMGMAKPELGGDPSLVDVIGWNFYPHNQWYFKGPTIPMGHHEYRPLADMLV
                     EMAERYRKPMFLSETGAEGSGRPAWLHYVCDEVRDAQSRGVSVEGICLYPVTAYPGWD
                     NSRHCEVGLLSTIGSDGKRKVYEPLREELQRQKAIFGLVEAGETRPSRLRIARS"
     gene            2111..2875
                     /locus_tag="VFU20_06805"
     CDS             2111..2875
                     /locus_tag="VFU20_06805"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEU5286201.1"
                     /translation="MRQNWPERLWLVRHGQSQGNVAREAAHEAGLAVIDIEMRDVDVP
                     LSELGFRQAEAAGRWFASLPPSERPEVILSSPYVRARQTAEAICKAGALAGGPARTIV
                     DERLREREFGVFDRLTTIGIREKFPEEAAHRRRLGKFYHRAPGGESWADVILRLRSML
                     NTINLHYNDRRVLVVCHQVVVLCMRYILEELDEAQILAIDKQADVLNCGICAYDFEPD
                     AEGLCTPRLALWNHDAPLEADGAVKTSAPDAMAGSR"
     gene            2872..3741
                     /locus_tag="VFU20_06810"
     CDS             2872..3741
                     /locus_tag="VFU20_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU5286202.1"
                     /translation="MNPGTPLDRTALDSNPLPPIVDGGKESKGRILIVAGSREVPGAA
                     LLVATAAMRAGAGKLRIATVASIAQQVGMAMPEAMVIALPEHRRSGFARSAVKQIADQ
                     AADADAIVAGPGVTHGETCRRIADRLLDSPARLALDAALLRSLSPLEEDELERETMPV
                     LLPHAGELAALLDTGEDEVAAHPVRCGLRAAQLYRSVVLVKGVTSHVVHPDGRSWTYR
                     GGAPGLGVSGSGDVLAGIVGGLLSRGADPLAALLWGVWLHGEAGAALAGKVAPIGFLA
                     REIADEIPALLPR"
     gene            complement(3780..5246)
                     /gene="pyk"
                     /locus_tag="VFU20_06815"
     CDS             complement(3780..5246)
                     /gene="pyk"
                     /locus_tag="VFU20_06815"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU5286203.1"
                     /translation="MTESFEPRSRKVKILATLGPASSDPAMIRALMLAGADAFRINMS
                     HGDHAQKAELVEAIRALERELKRPTTILFDLQGPKLRVGHFKGKSAMLKAGDRFTLDR
                     KHTAGGPDRVELAHPELFATIRPGDRILIDDGKVRLKTIEVDDRTLVTEVQVGGKVSD
                     NKGVNVPDVLVPIPSLTEKDRADLAFALEQKADWIALSFVQRPEDVAEARALIGEKAA
                     LLAKIEKPAAIDQLNDIIALADAVMVARGDLGVELPPEQVPPLQNRIIACARQFGKPV
                     VVATQMLESMISSPTPTRAEVSDVATAIYDGADAVMLSAESATGDYPCEAVAMMDRIA
                     SSAERDPIYPARIHFTETRLEPTTADALAGSARQIASTVSATAMVCYTSSGSTARRIA
                     RERPAVPLLAMSPSLTTARRMGLLWGVHPVHSRDVTSFEEMVEKGKRMALRHRIAQGG
                     DRVVLMAGVPFGTAGSTNVIHVVRLVGDELEKHTRSKG"
     gene            5376..5618
                     /locus_tag="VFU20_06820"
     CDS             5376..5618
                     /locus_tag="VFU20_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HEU5286204.1"
                     /translation="MAEGTVAADQLRLFIERIERLEEEKKAIADDIRDVYSEAKANGY
                     DTKTMRAIVRLRKMETHTRQEMDALLETYRTALGLA"
     gene            5628..6368
                     /locus_tag="VFU20_06825"
     CDS             5628..6368
                     /locus_tag="VFU20_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5286205.1"
                     /translation="MAGHSKFKNIMHRKGAQDKKRSAMFSKLAREITVAAKMGLPDPE
                     MNARLRAAVLAAKAQSMPKDNIQRSIDKATGGEAENYEEIRYEGFGPGGVALIVEALT
                     DNRNRTATNVRTIFSKNGGNLGAGGSVSHGFERMGLIEYGPGAGDSDKVLEAAIEAGA
                     EDVESDEDGHQVWTSVETLHAVARALEPMLGEADSTRLGWKPQMEVEVRGTEAEQLAR
                     LIEALEEDDDVQTVWGNEKIPDEELARL"
     gene            6365..6862
                     /gene="ruvC"
                     /locus_tag="VFU20_06830"
     CDS             6365..6862
                     /gene="ruvC"
                     /locus_tag="VFU20_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007407071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU5286206.1"
                     /translation="MKVLGLDPGLGTTGWGLIAADGNRLSHLANGELRTATSAPLPVR
                     LADLAGQLEALLAEHRPDAAAVEEVFVNKNPQSTLKLGQARGVALMCAARSGVTVGEY
                     AARLVKKAVVGTGNADKAQVHAMVGRLLPGVAIAGPDAADALAVAITHAHHLASARAT
                     QNRSP"
BASE COUNT         1113 a         2384 c         2382 g         1048 t
ORIGIN      
        1 gacgcgctgc cggaaaagcc ggcctgggcc caggccacgc aggatttctg ggcgccgagc
       61 gtgatctgcg acggctcgac ctatttccta tattattcgg cgacccccga tgtctgccac
      121 gaccccaggc gcgggcactg cctcgccgtc gcgacgtcgt cgtcaccggc cgggccgttc
      181 acagacatgg gcattccgct gctgcatggg atggggttcg aatatatcga cccgatggcc
      241 tatgacgatc cggtcacggg caagcgcctg ctctactggg gctcgggatt ccagccgatc
      301 aaggtccagg agctgtccga agatcgcctg tcgttcgaac cgggaagcca gccggtcgat
      361 ctcgtctggc ccaacccggt caagggcgcg ttcccgcgcc tggtcgaagc ctcgtgggtg
      421 atccggcacg acgatttcta ttatctcttt tactcgggcg acaattgctg cgggcccgat
      481 gccgactatg gcgtgatggt cgcccgttcg gccagcgcga ccggcccgtt cgagacgctc
      541 gaacaggcgc ggggcgttcc ccacagcctg atgcttttca ggagcgagcg ctggctggcg
      601 ccagggcaca acacggtcgt gaccgacaag gcggggcagg cctggatcat ctaccacgcg
      661 atcgacgtga accgcccgcg ccagcgccag gaggatgaga tcaacagccg ccggatcctg
      721 atgatggacc gcatcgaatg gcgcgacggc tggccgtatg tcggcacccc gtcggacgag
      781 ccccgggccg ggccggtggt ctaatccgat ttcggcggcc tgcgctgcgc agcgttacga
      841 tcgggcaatc cgcagccggc tgggccttgt ttcgcccgct tcgacaagcc cgaagatcgc
      901 tttttgccgc tgcagctctt cgcgcagcgg ctcatagacc ttgcgcttgc cgtccgagcc
      961 gatcgtcgag agcaggccga cctcgcaatg ccgcgaattg tcccaaccgg gataagcggt
     1021 gacgggatag aggcaaatgc cctccacgct gactccccgg ctctgggcgt cgcgcacctc
     1081 gtcgcaaaca taatgcagcc aggccgggcg gccggaccct tcggcgccgg tctcggaaag
     1141 gaacatcggc tttcgatacc gctccgccat ttcgacgagc atgtcggcga gcgggcgata
     1201 ttcgtggtgc cccatcggga tcgtcggccc tttgaaatac cattggttgt gcgggtagaa
     1261 gttccacccg atcacatcga cgagcgacgg gtcgccgccc agctcgggct tggccatgcc
     1321 catgatcgag tcataggcct cgtacatgcc ctggaggttc tgctcggcgg cgcggaccgt
     1381 ccggcggcgg cggtcgtgcg gggcgatgtg gatcaggggc tctgcccaga cgatcgtcgt
     1441 ttcgggccat tgctcgcgga tcgcgcgggc ggcggctatc gcggtcctca ggaggtggtg
     1501 cttgaaccag ccgcgccggc ccgggccaac cggcgggaaa tatccgtcat ccaccgccca
     1561 ggacaggaag ttgatctcgt tgagcgggca gacgatgggc ggccggttgc tgaccgactg
     1621 ctgcacctcc agcgccgcga ccgcgaaatc ggtgaagcgc tggggaaaat cgggccccgc
     1681 ctggtcgaca tggtcgggcg acccgtaatg gaacaggtcc cagatgacct ggaggcccag
     1741 ccgctcggag gcctcaaggg ccggcagcca gctcgaccag tcgtaccggc cgggagactg
     1801 ttcgatcaaa tgccagcgca gcccgtcccg aagcgtcctg aagccgagct ccaggcactg
     1861 ccggtaatcc gattccaggt gacggtcgtg cgacgtcgcc cggatcaggt cgaggcgaac
     1921 gccgtcgcgc cgccggtgcg acgaacattc gaagccggcc tggaaaaaac tggcgaacgt
     1981 cttgcctgga tggttgttca tggaagccac caatgcgcga aagtccaaat tgagcccagc
     2041 cgggcgcaga aacggcacga gggcacaatc gttcacttca agctttctca cgcctgaagg
     2101 agcaaggcac atgcgccaga actggcccga gcggctgtgg ctggtccggc acgggcaaag
     2161 ccagggcaat gtcgcgcgcg aggcggccca cgaggcgggg ctggcggtga tcgacatcga
     2221 gatgcgcgac gtcgacgtgc ccttgtccga gctcgggttc cggcaggccg aggccgccgg
     2281 ccgctggttc gcctcgctgc cgccaagcga gcggcccgag gtcatcctct cctcccccta
     2341 cgtccgggcg cgccagaccg cggaggcgat ctgcaaggcc ggggcgctgg ccggcgggcc
     2401 agcccggacc atcgtcgacg agcggctgcg cgagcgcgag ttcggggtgt tcgatcggct
     2461 gacgacgatc ggcatccgcg aaaaattccc cgaggaagcc gcgcaccgcc ggcggctggg
     2521 caaattctat caccgggcac cgggcggcga gagctgggcc gacgtcatcc tccggctgcg
     2581 ctcgatgctc aacaccatca acctgcatta caacgaccgc cgggtgctgg tcgtctgcca
     2641 ccaggtcgtg gtgctgtgca tgcgctacat attggaagag ctggacgagg cacagatcct
     2701 ggcgatcgac aagcaggccg acgtgctcaa ctgcggcatc tgcgcctacg acttcgagcc
     2761 cgacgccgag ggcctgtgca cccccaggct ggcgctgtgg aaccacgacg cgccgctcga
     2821 ggccgacggc gcggtcaaga cctcggcgcc ggacgccatg gccgggtcgc gatgaacccc
     2881 ggcacgccgc tcgaccggac cgcgctcgat tccaacccgc ttccgccgat cgtcgacggc
     2941 ggcaaggaga gcaagggccg gatcctgatc gtcgccggca gccgcgaggt cccgggcgcg
     3001 gctctgctcg tagcgaccgc ggcgatgcgc gcgggcgccg gcaagctgcg catcgccacc
     3061 gtcgccagca tcgcgcagca ggtcggcatg gcgatgcccg aggcgatggt gatcgcgctt
     3121 cccgagcatc gccgcagcgg ctttgcgcgc tccgcggtca aacagattgc cgaccaggcc
     3181 gccgatgccg acgcgatcgt cgccggcccg ggcgtgaccc acggcgagac ctgccggcga
     3241 atcgccgacc ggctgctcga ctcgccggcg cgcctggcgc tcgacgcggc gctgctgcgc
     3301 agcctgtcgc cgctcgagga ggacgagctc gagcgcgaaa ccatgccggt gctgctgccc
     3361 cacgccggcg agctcgccgc cttgctcgac acgggcgagg atgaggtcgc cgcccacccg
     3421 gtccgctgcg gcctgcgcgc cgcgcagctc taccgctcgg tggtgctggt caagggcgtc
     3481 accagccacg tcgtccaccc cgacggccgc tcgtggacct accgcggcgg cgcgcccggg
     3541 ctcggtgtgt cgggcagcgg cgacgtgctc gccgggatcg tcggcggcct gctgtcgcgc
     3601 ggcgccgacc cgctcgccgc cttgctgtgg ggcgtgtggc tgcacggcga ggcgggggcg
     3661 gccctggccg ggaaggtcgc gccgatcgga ttcctcgccc gcgagatcgc cgacgaaatc
     3721 ccggcgctac ttccgcgctg accccttgcc gacagcgtgt ttttcgagct cgaaaagcgt
     3781 caccctttgg aacgggtgtg tttttcgagc tcgtcgccga ccagccggac gacatggatg
     3841 acgttggtcg agcccgcggt gccgaacggc actccggcca tcagcaccac ccggtcgccg
     3901 ccctgggcga tccggtgccg cagcgccatc cgcttgccct tctcgaccat ctcctcgaag
     3961 ctggtgacgt cgcgcgagtg gaccgggtgc acgccccaca gcagccccat gcgccgggcg
     4021 gtggtcagcg acgggctcat cgccagcagc ggcaccgccg gccgctcgcg ggcgatccgc
     4081 cgcgcggtcg agcccgagct ggtgtagcag accatcgcgg tcgccgagac ggtgctggcg
     4141 atctgccgcg ccgagccggc gagcgcgtcg gcggtggtcg gctcgagcct cgtctcggtg
     4201 aaatggatcc gcgccgggta gatcgggtcg cgctcggcgc tcgaggcgat tcggtccatc
     4261 atcgccaccg cctcgcacgg atagtcgccg gtcgcgctct cggccgacag catcaccgcg
     4321 tcggcgccgt cgtagatcgc ggtggcgacg tcgctgacct ccgcccgggt cggggtcggc
     4381 gagctgatca tcgattcgag catctgcgtc gccaccacca ccggtttgcc gaactggcgg
     4441 gcgcaggcga tgatccggtt ctggagcggc ggcacctgct cgggcggaag ctccacgccg
     4501 aggtcgccgc gcgccaccat caccgcgtcg gcgagcgcga tgatgtcgtt caactggtcg
     4561 atcgccgccg gcttctcgat cttggcgagc agggcggctt tctcgccgat cagcgcccgc
     4621 gcctcggcga cgtcctcggg ccgctggacg aacgacagcg cgatccagtc ggccttctgc
     4681 tcgagcgcga aggcgaggtc ggcgcggtcc ttttcggtca gcgacgggat cggcaccagc
     4741 acgtcgggga cgttgacgcc cttgttgtcg gagaccttgc cgccgacctg gacctcggtg
     4801 accagggtcc ggtcgtcgac ctcgatcgtc ttcagccgca ccttgccgtc gtcgatcagg
     4861 atccggtcgc cgggccggat ggtcgcaaac agctcggggt gggcgagctc gacccggtcc
     4921 gggccgccgg ccgtatgctt gcgatcgagc gtgaagcggt cgccggcctt gagcatcgcc
     4981 gacttgccct tgaaatgccc gacccggagc ttggggcctt gcaggtcgaa caggatggtg
     5041 gtcggccgct tcaactccct ttcgagcgcc cggatcgcct cgaccagctc ggctttctgc
     5101 gcgtggtcgc cgtggctcat gttgatccgg aacgcgtcgg ccccggccag catcagcgcg
     5161 cggatcatcg ccgggtcgct cgacgccggc cccagcgtcg ccaggatctt caccttgcgc
     5221 gatcgcggtt cgaacgattc cgtcacgggg ctccttccaa cgcgctcgaa gcggcccggc
     5281 catagccggt tggcgcgctg gttcaacccg gcttgagcag ccgccgccgc tcgcctagag
     5341 ccgcgcgctg aaccctgaac tggagagtag gaactatggc ggagggcacc gtcgcggccg
     5401 atcagctgcg gctgttcatc gagcgcatcg agcggctcga ggaagagaaa aaggcgatcg
     5461 ccgacgacat ccgcgacgtc tattcggaag ccaaggcgaa cggctacgac accaagacca
     5521 tgcgggcgat cgtccgactg cggaagatgg agacccacac ccgccaggaa atggacgcgc
     5581 ttctggagac ctatcgcacg gcgctcgggc tggcctaaga gaccgagatg gccggccatt
     5641 ccaaattcaa gaacatcatg caccgcaagg gcgcgcagga caaaaagcgc tcggcgatgt
     5701 tctccaagct cgcccgagag atcaccgtcg cggccaaaat gggcctgccc gacccggaga
     5761 tgaacgcccg gcttcgcgcc gcggtgctgg ccgccaaggc gcagtcgatg cccaaggaca
     5821 atatccagcg ctcgatcgac aaggccaccg gcggcgaggc cgagaattac gaggaaatcc
     5881 gctacgaggg cttcggcccc ggcggggtcg cgctgatcgt cgaggcgctg accgacaacc
     5941 gcaaccgcac ggcgaccaac gtccgcacca tcttctccaa gaacggcggc aacctcggcg
     6001 ccggcggcag cgtcagccac ggcttcgagc gcatggggct gatcgaatat gggcccgggg
     6061 cgggcgactc ggacaaggtc ctcgaagcgg cgatcgaggc gggggccgag gacgtcgaat
     6121 cggacgagga cggccaccag gtctggacca gcgtcgaaac gctgcacgcc gtcgctcggg
     6181 cgctcgagcc gatgctcggc gaggccgaca gcacccgcct cggctggaag ccccaaatgg
     6241 aggtcgaggt gcgcgggacc gaggccgagc agctcgcccg gctgatcgag gcgctcgagg
     6301 aggatgacga cgtccagacc gtctggggca acgagaagat ccccgacgag gagctggctc
     6361 ggttgtgaag gtcctcggcc tcgacccggg gctgggaacc accggctggg ggctgatcgc
     6421 ggcggacggc aaccggcttt cccacctcgc caacggcgag cttcggaccg ccacctcggc
     6481 cccgctgccc gtgcggctcg ccgacctcgc cggccagctc gaggcgctgc tcgccgagca
     6541 ccggcccgac gccgcggcgg tcgaggaggt gttcgtcaac aagaacccgc agtcgacgct
     6601 caagctcggc caggcgcgcg gggtcgcgct gatgtgcgcg gctcgctcgg gggtcaccgt
     6661 cggcgaatat gcggcgcggc tggtcaagaa agcggtggtc ggtaccggca atgccgacaa
     6721 ggcgcaggtc cacgcgatgg tcggccggct gctgcccggg gtggcgatcg ccggccccga
     6781 cgccgccgat gcgctggccg tcgcgatcac ccacgcgcac catctggcgt cggcccgagc
     6841 cactcaaaac cgttcgccct gagcctgtcg aagggctgcc tttctctcta aaggacgttg
     6901 aacaaagggc agggcttcga caagctc
//
LOCUS       DASTZO010000246        18184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_908566,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000246 DASTZO010000000
VERSION     DASTZO010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 18184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18184
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908566"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1914
                     /gene="lepA"
                     /locus_tag="VFU20_06835"
     CDS             94..1914
                     /gene="lepA"
                     /locus_tag="VFU20_06835"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU5286207.1"
                     /translation="MATDAAHIRNFSIIAHIDHGKSTLADRLIQATGGLTSREMTAQV
                     LDNMDIERERGITIKAQTVRLHWQGYELNLMDTPGHVDFAYEVSRSLAACEGALLVVD
                     AAQGVEAQTLANVYQSIEHDHEIVPVINKVDLPAAEPERVREEIEEIIGLDASHAVLT
                     SAKTGEGVEAVLQAIVDVIPPPKGDAAAPLKAMLVDSWYDPYLGVVILVRVIDGVIRK
                     GQQVKFMAAGTTHLVDRVGCFRPKIEQLSELGPGEIGFITAQIKEVAETRVGDTITDA
                     RHPTAHALPGFKLVQPVVFCGLFPADAAEFEKLRDSLYKLRLNDASFSFEMETSAALG
                     FGFRCGFLGLLHLEIIQERLTREYDLDLITTAPSVVYKVHLAHSKSEAAQIIDLHNPA
                     DMPDPNRIESIEEPWIEATIYVPDDYLGSVLKLCQDRRGIQKNLTYVGHGKSSRALLT
                     YELPLNEVVFDFYDRLKSISRGYASFDYHMVGHRPGDLVKMSILVNGEPVDALSMIVH
                     RANAESRGRGMCERLKELIPRHLFKVPIQAAIGGKIIARETIAALRKDVTAKCYGGDA
                     TRKRKLLEKQKEGKKRMREYGSVSIPQEAFIAALRMGDDA"
     gene            2037..4445
                     /locus_tag="VFU20_06840"
     CDS             2037..4445
                     /locus_tag="VFU20_06840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5286208.1"
                     /translation="MAKRADRDLGASGGIAGAAPPAARAAATENLVMPDLPPEMFSPL
                     GLLALADLLPVMTAYVDKEQRYRFLNKPLADWFERPRSEILGRTLKEVLGDKAYGVRE
                     PLIAAALAGERQYFASEFDHPTRGALAVQTNYVPWLDHGEVKGLVIVVADVTEQRAAE
                     RALRESEARFRRIADSAPVMMWVTRLDRSRDFVNDAYTEFAGLSREEARRFDWRSIIH
                     PDDAERVIAESLAGEASGKPFALEARYRRHDGEYRWLRSVSSPRFGAEGELAGFIGVA
                     SDITLAKEAELELKREVEERTAALRESEARFRAIFETVLEVLVLLKPDGTIVELNRKE
                     AVWRDKNYDRAIGGKIWDAPTLQAYPQHKPLIKRAVRQAAKGVLFNEEVTMEREGVPT
                     AYLDVSVQPVRDERGKIIYLLFEARDITDLKATQEQLRQSQKMEALGQLTGGIAHDFN
                     NLLTVVVGGLDIVAKRAEDEKLRRYAENALSAAERGARLTGQLLAFSRVQRLEVRPTP
                     VAPLIVNMRPLLRNVLGPGIEKRFSLDDEELPVMADPTQLEVALLNLAINARDAMPDG
                     GVLTFATRSVMVENDPELGDGRYVELSITDTGTGMSEEVAARAFEPFFTTKEVGKGTG
                     LGLSMVYGMARQSGGSAQIVSPPGGGTSVKLLFRVAEQPAAAAGPERADPEREESAAR
                     DASILVIDDDPDVRGFIVNSLEEQGYRVAAASDGRKGLKAFATERPDLVILDFIMPGL
                     SGAEVANRIRADAPNQPILFVSGYSETEAVRRVAPDAPLLTKPFRADALDKAVRGTLA
                     GNRA"
     gene            complement(4464..6017)
                     /locus_tag="VFU20_06845"
     CDS             complement(4464..6017)
                     /locus_tag="VFU20_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286209.1"
                     /translation="MALSMRAGERLSIRGTAKFGLLTGLCAIFIDAAIAHGLFWENDP
                     YWTYWITKSFLIATVFIIGTAFLGIGLVQGFLITAVHTAILEIYYEWLAPIGLPQEPQ
                     WLDDNHLWVTGVPVHYLAILIGYLMALWIWRRADPVREGGSARSADSAGARATPAALA
                     VSALVATAVILVLSGVITHAILLGEFPGITYFVQHLLIGFVFLYLWSAYVGAGERGWI
                     VGALMLSLVWTTYGIYLGPVGLPEKVQYLTHHQHWSRVFPGDFLAALIGLFIAIRLLP
                     SLSARVPAAALSLLALALLAPDSAVAKPRGLHAGAAASGQAHRVLGPNPVDFRSTQPA
                     NGSISIKVVEGGNRWSHVQARDAIELAAEFTASDGRYRIVVDRAMPRHPLGAYTTWNG
                     VALNHEMHGETGIGTSKLPLMMPEISLYGWGKVWRNGQLVAAMTPVHAMVSTGGSMPG
                     VMLEVDTEEKTLRDVAGGYLTVYWPTVEGLRMPRETLRNVQILGWVGLIGLALLFFLL
                     ARNAERRRA"
     gene            6231..6917
                     /locus_tag="VFU20_06850"
     CDS             6231..6917
                     /locus_tag="VFU20_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HEU5286210.1"
                     /translation="MRQWYRRRYLDLLGSIYIYNEHRGYTAIDRVLEAVRTRWPDDHA
                     LIAQIAKHRADERKHYLMFRRWFERRGVMPFAVDRTCGHIDRFVGIMFRSKIDDLDPR
                     LIIGRDYLFERLCRVISLTEKRGYRQVEILLRNAAVRGDKRLVKIFQIIREDEPDHWA
                     PYEGWLQAHGKRRDSWWERAIDGFIHSELLLVKLPILFLTPRLRRRSEWADAHERVPP
                     DGYPELKQAT"
     gene            6914..7327
                     /locus_tag="VFU20_06855"
     CDS             6914..7327
                     /locus_tag="VFU20_06855"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEU5286211.1"
                     /translation="MSALARRLFSRNAAVLLARNTVVSCLVFVIGLALLWALVELWDV
                     DKLVAAAAGFLVSNSIHYVFGRKWIYRGTERAVASGYAYFLVNAGIGLVVTVTLFAAF
                     LRWTSLNYLVARVVVSVFAGLAMFLLNAMLNFKRL"
     gene            7334..8467
                     /locus_tag="VFU20_06860"
     CDS             7334..8467
                     /locus_tag="VFU20_06860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU5286212.1"
                     /translation="MRVALFSGNYNYLREGANQALNRLARDLEADGHEVRAYSPVTDR
                     PAFEPAGTLVPVPSIPLPVRSEFRLSLGLPRAVREDVRRFAPDIVHLSTPDILGTRAQ
                     TFARRLGIPTVASLHTRFEAYLDYYGLGWAGPLVEAHLRRFYRRCDHVLAPTPALVAA
                     MKAMRGDDQVSLWSRGVDRALFNPARRDPEWRRSNGWSDEDVVVLFFGRLVAEKGIDR
                     YVSTLRQLQRRSTGIRALAVGAGPAAGAFDALPGAVLTGHLDGAELVRAVASADILLH
                     PSTTETFGNVVLEAMASGLAVVCARTDSAEALVEHGRTGILCAPDDCEGYRQAILALA
                     EDPRRRSDLGEAARRSSEAFSWSNASRSVAQAYRDVLGRRRRR"
     gene            8539..8775
                     /locus_tag="VFU20_06865"
     CDS             8539..8775
                     /locus_tag="VFU20_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286213.1"
                     /translation="MDQFQSDAEYFARRSNEERAAAERATHPRARQSHIDLADRFAEA
                     ARAIAEVSTLWEADIGQERPVAVSARQPEFRILP"
     gene            complement(8788..11094)
                     /locus_tag="VFU20_06870"
     CDS             complement(8788..11094)
                     /locus_tag="VFU20_06870"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5286214.1"
                     /translation="MISARLPGGESLGLTSREGDGESSPLKDVQLISRAQLAPFFAAA
                     NVVAALMMSAILWGEVDVTWLLPWCGTVAIANLAAMQLARTHAITHVGRSGRQVPQWL
                     MVGDVAGRALLWLSLPLAMFPSLQPGTQVIAASLAAGLGIGALGLVVVPPCVTAWLSA
                     FTAGVSVALLFGRNSVPFPHMLSILFTLGVAIFGVLAVTRWAFHQLKTNADFGSQSES
                     ASLLLQEYEQRGVGWLWQVDSENRVTYISSRMTALLGRPASQLLGHSLPSLLGGNAEL
                     GRLLLERQPFNSLEMELKTARGERWISIAGDPIIDTAGRFEGFRGVGSDITEIRQTQE
                     RLTHLANVDVLSGLPNRGRVRQLLGDALRAATVSNVPCAILFLDLDGFKPVNDTFGHP
                     KGDAVLRAVAKRLVDEVGSGGHVGRMGGDEFAIVVIDAQSRRNVEAMADRIIKAIAEP
                     YMIDQTEIRIGVSIGCAFGPIDGATVDDLILKADLALYEAKGAGRGIARYFSSELQSE
                     QEDRVRLETDLRAAIAGKQFHLAFQPLVNAKDQKLIGFEALIRWNHPQRGLVPPNVFI
                     PVAEEAGLMPVIGEWVIDEACRAAATWPEPITVALNISPKQIALAALPNTVSEALARH
                     KVPGNRIELEVTEGVFLGDNGSTLDVLKRLRQLGVGIALDDFGTGYSSIGYLNKAVFH
                     KLKIDGSFVREAGTRPENVAIIQSIVQLAKSFRMSVTAEGVETAEDFERMRDLGCDTI
                     QGYLFGRPLSYERANQMVIGLSAKRLAG"
     gene            complement(11200..13131)
                     /locus_tag="VFU20_06875"
     CDS             complement(11200..13131)
                     /locus_tag="VFU20_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5286215.1"
                     /translation="MKFWSRAALLAAMILTAACRVSEQDNAVQPAASETATAIAPILA
                     TPDARDIHSHARPQEARVHHVALDLAVDFDGKRIGGTATLDLDRRPGAKEIVLDDKGL
                     EIQAITDGKGEPLGWKVGAADANLGAPLAVALRPDTKRLVIRYRSAPDAEALQWLTPQ
                     QTAGKKRPYLFSQGQAILNRSWIPTQDSPGIRQSWEATIRVPAGLTAVMSAPRSAEPI
                     TQGGESVFEFRMRNSVPPYLIAIAVGDLAFKPLGPRSGVWTEPAMLDSAAAELADTEK
                     MIDSAEKLYGPYLWERYDMLVLPPSFPFGGMENPNLTFLTPTFIAGDKSLVSLIAHEL
                     AHSWSGNLATNATWADFWLNEGMTTYATTRIVEAIYGPKVAAQQIALGVDSLNEEIET
                     LGGPSAPDTRLHIDLKGRNPDDGLTEIAYEKGAAFLRTIEAAVGRDRFDAYLKGWFER
                     HRFQPVTSAIFLADLRQHLIKGDQALERKLMLDRWVYQPGLPPNMVKPDPRAFAEADR
                     AVAAFAAGGAPGTEAWSRWTTDERLRFLNRLPRKLPTARLDALNRAFGLNAVRNLEVR
                     FAWLELAVGNRYEPAVPSLEQFLTTQGRRKFVRPLVKALAEDSAWGRPIAARIYPLGR
                     PLYHPIVTRELDALKLGGG"
     gene            complement(13143..13697)
                     /locus_tag="VFU20_06880"
     CDS             complement(13143..13697)
                     /locus_tag="VFU20_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5286216.1"
                     /translation="MDEGTQQDWAVIATQFMAYAAGLPDRVLAHLRLLDGDFGGFPVD
                     RLQHSLQTATRAHRAGEAEDYVVMALIHDIGDTLGSYNHPDIAAAILKPFVDDRLHWI
                     AEKHGIFQGYYFFHYIGLDRDMREAFRGHPYFEDTARFCETYDQAAFDPDYESAPLDF
                     FEPMVRRVFAAPKRSLYVGIAESG"
     gene            13813..14991
                     /locus_tag="VFU20_06885"
     CDS             13813..14991
                     /locus_tag="VFU20_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5286217.1"
                     /translation="MATDSAALERQQRPARRAARPGAPRIQSPAAPIAEGIGLDQPFF
                     ADKNRAFWILQSIGWSGYFILRTLSGFANSMGWMWIVHTLLLTATGYSLTLLLATLFR
                     RLIAMRALLTVALSLAAVMIAASAFSIIETWSYATFLKPNARPVGFEYLGAILLNFSL
                     LAAWAALYYGINYFLLLEEQIDQRERLESQASTAQLAMLRYQLNPHFLFNTLNSISTL
                     VLLKQTERANAMLARLSSFLRYTLVNEPTAKVTLAQEVETLKLYLEIEKMRFEDRLRP
                     HFKIESETIGARLPSLLLQPLIENAIKYAVTASESGADIWITAHRQGESVRIEVADNG
                     DGEGAALAASPSTGVGLANIRDRLAQAYGAAHAFTTRKNERGGFSVIIEIPFESGDRD
                     "
     gene            14991..15797
                     /locus_tag="VFU20_06890"
     CDS             14991..15797
                     /locus_tag="VFU20_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU5286218.1"
                     /translation="MTIRTVLVDDEPLATQGLQLRLQAHEDVEVVATAANGREAIRAI
                     KTHKPDLVFLDIQMPGFDGFSVIQGLMEVEPPLFVFVTAYGDHALRAFESQAVDYLMK
                     PVDEDRLAATLDRVRQRLVEKRGAEEAERLKEVLVEHAPEAAESLAEETAGDAPAANR
                     FERMINIKDQGQIFRVDVDTIERIDAAGDYMCIQTGDNTLILRETMKDLEKRLDPRRF
                     QRVHRSTIVNLDLVRQVKPHTNGECFLVLDSGAQVKVSRSYRDVVARFVH"
     gene            15967..16509
                     /locus_tag="VFU20_06895"
     CDS             15967..16509
                     /locus_tag="VFU20_06895"
                     /inference="COORDINATES: protein motif:HMM:NF019320.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme NO-binding domain-containing protein"
                     /protein_id="HEU5286219.1"
                     /translation="MKGLVFTTFYRHCESRYGADFLDDLIEEAALPNQGAYTSVGSYP
                     FEEMVALVTALASKTGTPMPVILREFGEFCFLQWVRYVPAHFDGKQLFDIMAGIDGFH
                     ENEVRKLYPDAELPSFKVESRDGDRMILRYHSCKPLADLAVGVIHGAANYLGETVEVH
                     HQPMAEPRGPYVRIEINRVS"
     gene            16547..18082
                     /locus_tag="VFU20_06900"
     CDS             16547..18082
                     /locus_tag="VFU20_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5286220.1"
                     /translation="MDDRLRRLELRVAREHAARREAESILESKSLELFAANQRLLQLN
                     MELEERVARRTEELDRARRSALQLVENDHLTKIPSRFRFSRALHESLERTLQTGGSIG
                     LLLIDIDDFKLVNDCYGHGHGDLLLKEIAARLRSIVRRHELVARLGGDEFAVILEGSA
                     AASLERGARRFQAAFVEPVTVGGVTLKIAGSMGLATSPEDATTSADLQRFADLALYQV
                     KARGGGGFAQFEKGLVDSYDRRRRLEAEFRGAVANDAIHLWFQPIVEIRTGETIGVEA
                     LARWQDSQHDAIGPDFFIPLAEQCGLIREVGRHLLGKALAAAKPWLDDGLIRKLTFNI
                     SPLELLDDGFADEILESLAAAQVEPAKLALEITEGVLIKNLSLAKAVMARLRRAGVKF
                     ALDDFGCGYTNLSALTNLPISILKIDRTLLRNVEHDESARVIISNVVNLCRDLGIRSV
                     CEGAETAAQLAFLDLIGCNSVQGYVCARPASAQDTEIFLRRAVQRRRDPGLRPRGPKS
                     AVA"
BASE COUNT         3029 a         6073 c         6231 g         2851 t
ORIGIN      
        1 ggattccgcg ggtgtcctcg cgcgcgcgga cgaggcgctg tacgcggtga agggtcaggg
       61 gcgcgacggc gtcgcgctcc tatcctgagt gccttggcca ccgacgccgc gcacatccgc
      121 aacttctcga tcatcgccca catcgaccac ggcaagtcga ctctcgccga ccggctgatc
      181 caggcgaccg gcggcctgac cagccgcgag atgaccgccc aggtgctcga caatatggac
      241 atcgagcgcg aacgcgggat caccatcaag gcccagaccg tgcggctgca ctggcagggc
      301 tacgagctca atctgatgga cacgcccggc cacgtcgact tcgcctacga ggtgtcgcgc
      361 tcgctggccg cgtgcgaggg cgctttgctg gtggtcgacg ccgcgcaggg ggtcgaggcg
      421 cagaccctgg ccaacgtcta ccagtcgatc gagcacgacc acgagatcgt gccggtgatc
      481 aacaaggtcg acctgccggc cgccgagccc gagcgggtgc gcgaggagat cgaggagatc
      541 atcggcctcg acgcgtccca cgcggtgctg accagcgcca agaccggcga gggcgtcgag
      601 gcggtgctgc aggcgatcgt cgacgtcatc ccgccgccca aaggcgacgc cgccgcgcct
      661 ctgaaagcga tgctggtcga cagctggtac gacccgtacc tcggcgtcgt catcctcgtc
      721 cgggtgatcg acggcgtcat ccgcaagggc caacaggtca agttcatggc cgccggcacc
      781 acccacctgg tcgaccgcgt cggctgcttc cgccccaaga tcgagcaatt gtccgagctc
      841 gggcccggcg agattggctt catcaccgcc cagatcaagg aagtcgccga gacccgcgtc
      901 ggcgacacca tcaccgacgc caggcacccg accgcccacg cgctgcccgg cttcaagctc
      961 gtccagccgg tcgtcttctg cggcctgttc ccggccgacg ccgcggagtt cgagaagctc
     1021 cgcgacagcc tttacaagct gcgcctcaac gacgcctcgt tcagcttcga gatggagacc
     1081 agcgccgcgc tcggattcgg cttccgctgc ggctttttgg gcctgctcca cctcgagatc
     1141 atccaggagc ggctgacccg cgagtacgac ctcgacctga tcaccaccgc gccgagcgtc
     1201 gtctacaagg tccacctcgc ccactcgaaa tcggaagccg cgcaaatcat cgacctccac
     1261 aacccggccg acatgcccga ccccaaccgc atcgagagca tcgaggagcc gtggatcgag
     1321 gcgaccattt acgtccccga cgactatctc ggctcggtgc tcaagctctg ccaggaccgc
     1381 cgcggcatcc agaagaattt gacctacgtc ggccacggca agtccagccg cgctttgctc
     1441 acctacgagc tgccgctgaa cgaggtggtg ttcgattttt atgatcgttt gaaatcaatt
     1501 tcgcgaggct atgcctcctt cgactatcac atggtcggcc accgccccgg cgacctcgtc
     1561 aagatgagca tcctggtcaa cggcgagccg gtcgacgcgc tgtcgatgat cgtccaccgc
     1621 gccaacgcgg aaagccgcgg ccgcggcatg tgcgagcgcc tgaaggagct gatcccgagg
     1681 cacctgttca aggtcccgat ccaggcggcg atcggcggca agatcatcgc gagggagacc
     1741 atcgcggcgc tgcgcaagga cgtgaccgcg aaatgttacg gcggcgacgc gacgcgcaag
     1801 cgcaagctgc tggagaagca gaaggaaggg aagaagcgca tgcgggagta tgggagtgtg
     1861 tctattcccc aagaagcatt catcgccgcc ctccgcatgg gggacgacgc ctaattcctc
     1921 tccgctagtg gagggcggcg atcgaagggt aagggcccgc ggcggttcgc aaatcttgcc
     1981 gccccgctaa ccgtaacacc gtgactctgc gcgcgaatgc cgcaattatg gagcggatgg
     2041 cgaagcgggc ggacagggat ctcggagcat cgggcgggat tgccggcgcg gccccgcctg
     2101 ccgcgcgcgc cgccgcgacc gagaacctgg tcatgcccga ccttccgccc gagatgttct
     2161 cgccgctcgg gctgctggcg ctggccgacc tgctgccggt gatgaccgcc tatgtcgaca
     2221 aggagcagcg ctaccggttc ctgaacaagc cgctcgccga ctggttcgag cggccgcgct
     2281 cggagatcct cgggcggact ctcaaggagg tgctcggcga caaggcctat ggcgtgcgcg
     2341 agccgctgat cgccgcggcg ctggccggcg agcggcagta tttcgcctcc gagttcgacc
     2401 atccgacccg cggggcgctc gcggtgcaga ccaattacgt gccgtggctg gaccatggcg
     2461 aggtcaaggg gctggtgatc gtcgtcgccg acgtcaccga gcagcgggcg gccgagcggg
     2521 cgctgcgcga gagcgaggcg cggttccggc gaatcgccga ttcggcgccg gtgatgatgt
     2581 gggtgacgag gctcgaccgc agccgcgact tcgtcaacga cgcctacacc gagttcgccg
     2641 gcctgtcgcg cgaggaggcg cggcggttcg actggcgctc gatcatccac cccgacgacg
     2701 ccgagcgggt gatcgccgag agcctcgccg gcgaggcgag cggcaagccg ttcgcgctcg
     2761 aggcccgcta tcgccgccac gacggcgagt accgctggct cagaagcgtg tcgtcgccgc
     2821 gcttcggggc cgagggcgag ctcgccggct tcatcggagt cgccagcgac atcacgcttg
     2881 ccaaggaggc cgagctcgaa ttgaagcgcg aggtcgagga acgcaccgcg gcgctgcgcg
     2941 agagcgaggc ccggttccgg gcgatcttcg agacggtctt ggaagtcctg gttttgttga
     3001 aaccggatgg gaccatcgtc gagctgaacc gcaaggaagc ggtgtggcgc gacaagaatt
     3061 acgaccgggc gatcggcggc aagatctggg acgcgccgac cctgcaggcc tatccgcagc
     3121 acaagccgct gatcaagcgc gcggtcaggc aggcggcgaa aggcgtgctg ttcaacgagg
     3181 aagtgacgat ggagcgcgaa ggcgtcccga ccgcctacct cgacgtctcc gtccagccgg
     3241 tgcgcgacga gcggggcaag atcatctacc tgctgttcga ggcccgcgac atcaccgacc
     3301 tcaaggccac ccaggagcag ctccgccaga gccagaagat ggaggcgctc ggccagctga
     3361 ccggaggcat cgcgcacgac ttcaacaatt tgctgacggt ggtcgtcggc ggcctcgaca
     3421 tcgtcgccaa gcgcgccgag gacgagaagc tccggcgcta tgccgagaac gcgctgtcgg
     3481 cggccgagcg cggcgcccgg ctgaccgggc aattgctcgc cttcagccgg gtccagcggc
     3541 tcgaggtgcg cccgaccccg gtcgctcccc tgatcgtcaa catgcgcccg ctgctgcgca
     3601 acgtgctcgg cccggggatc gagaagcgat tcagcctcga cgacgaggag ctgccggtga
     3661 tggccgaccc gacccagctc gaggtcgcgc ttctcaacct ggcgatcaac gcccgcgacg
     3721 cgatgccgga cggcggcgtg ctgaccttcg cgacccgctc ggtgatggtc gagaacgacc
     3781 ccgagctggg cgacggccgc tatgtcgaat tgtcgatcac cgacaccggc accggaatga
     3841 gcgaggaggt cgcggcgcgc gcgttcgagc ccttcttcac caccaaggaa gtcggcaagg
     3901 gcaccgggct cggcctgtcg atggtctacg gaatggccag gcaatcgggc ggcagcgccc
     3961 agatcgtcag cccgccgggc ggcgggacca gcgtcaagct cctgttccgc gtcgccgagc
     4021 agccggccgc ggccgccggt cccgagcggg cggacccgga gcgcgaggaa agcgcagccc
     4081 gggacgcgtc gatcctggtc atcgacgacg accccgacgt ccgcggcttc atcgtcaaca
     4141 gcctcgagga gcagggctat cgggtcgccg cggcgagcga cggccgcaag ggtctgaagg
     4201 cgttcgcgac cgagcgtccg gacctcgtga tcctcgactt catcatgccc ggcctgtcgg
     4261 gcgccgaagt cgccaaccga atccgcgccg acgcgccgaa ccagccgatc ctgttcgtct
     4321 cgggctacag cgaaaccgag gccgtccgcc gggtcgcgcc cgatgcgccg ctgctgacca
     4381 agccgttccg cgccgacgcg ctcgacaagg ccgtgcgcgg cacgctcgcc ggcaaccggg
     4441 catagcccgg gccgcggcgc cgcctaggcg cgccgccgtt cggcgttgcg ggcgagcagg
     4501 aaaaacagca gcgcgaggcc gatcagcccc acccagccga gaatctggac gttgcgcagt
     4561 gtctcgcgcg gcatcctcag cccctcgacg gtcggccagt aaacggtgag atagccgccg
     4621 gcgacgtcgc gaagcgtctt ttcctcggtg tccacctcga gcatcacgcc cggcatcgag
     4681 cccccggtgg agaccatcgc atggaccggg gtcatcgccg cgacgagctg cccgttccgc
     4741 cacaccttgc cccagccgta gagcgagatc tcgggcatca tcagcggcag cttcgaagtg
     4801 ccgattccgg tctccccgtg catctcgtga ttgagggcga caccgttcca ggtggtgtag
     4861 gctcccagcg gatggcgcgg catggcccgg tcgacgacaa ttcgatagcg gccgtcggaa
     4921 gccgtgaact cggcggcgag ctcgatcgcg tcgcgggcct gcacatggct ccagcggttg
     4981 ccgccctcca ccacctttat gctgatcgag ccgttcgcgg gctgcgtcga ccggaagtcg
     5041 accgggttcg ggcccagcac ccggtgcgcc tggcccgacg ccgccgcgcc cgcgtggaga
     5101 ccgcggggct tggcaacggc cgagtccgga gccagcagag cgagcgccag cagcgacaag
     5161 gctgcggccg gcacgcgcgc cgaaagactc ggcagcagtc gaatggcgat gaacagtccg
     5221 ataagggctg cgaggaagtc gcccgggaag acccgcgacc aatgctggtg gtgggtcagg
     5281 tactgcacct tttcgggaag gccgaccggc ccgagataga tgccgtacgt ggtccagacg
     5341 aggctgagca tcagtgcgcc gacgatccac ccgcgctcgc cggcgccgac ataggccgac
     5401 cagaggtaga ggaagacgaa gccgatcagc aagtgctgga cgaagtaagt gatgcccggg
     5461 aactcgccga gcaggatcgc gtgggtgatg accccgctga gcaccaggat gacggcggtc
     5521 gccaccagcg cggagacggc gagcgcggcc ggagtcgctc gggcaccggc ggagtcggcc
     5581 gagcgagcgg agccgccttc ccgcaccggg tcggcgcggc gccagatcca cagcgccatc
     5641 agatagccga tcaggatcgc gaggtaatgg accgggacgc cggtcaccca gagatggttg
     5701 tcgtcgagcc actgcggctc ctgcggaagg ccgatcgggg ccagccattc gtaatagatt
     5761 tcgaggatcg ccgtgtgcac ggcggtgatc aggaatccct ggacgaggcc gatgccgagg
     5821 aaggcggtgc cgatgatgaa gacggtggcg atcaggaagg atttggtgat ccagtaggtc
     5881 cagtaggggt cgttctccca gaacagtccg tgcgcgatcg ccgcgtcgat gaagatcgcg
     5941 cacagccccg tcagaagacc gaacttggcg gtgccgcgga tcgaaaggcg ttcgccggcg
     6001 cgcatcgaaa gagccatggg aaagcctcct gttgtccgag gattcgacgc ggcggagcgg
     6061 cgattggttc ctcaggcggc ccgcatcagc tcatccttga atcgcacggc ctggcgcccc
     6121 gcaacctgtc gccgaatggg cgatcgctac gggaggccag gttcacagcg gcgcccggtc
     6181 gaaaattcac tgaatttgac tcgcggcgcg caacacctga tcgtgccgcc atgcgtcagt
     6241 ggtaccgccg tcgctatctc gacctgttgg gctcgatcta catctacaac gagcatcgcg
     6301 gctataccgc gatcgaccgg gtgctcgagg ccgtccggac gcgctggccc gacgaccatg
     6361 cgctgatcgc gcagatcgcc aagcaccgcg ccgacgagcg caagcactat ctgatgttcc
     6421 gccgctggtt cgagcggcgc ggggtgatgc cgttcgcggt cgaccgcacc tgcggccaca
     6481 tcgaccgctt cgtcgggatc atgttccgct cgaagatcga cgacctcgac ccgcggctga
     6541 tcatcggccg cgactatctg ttcgagcgat tgtgccgggt catctcgctg accgagaagc
     6601 gcggctaccg gcaggtcgag atcctgcttc gcaatgcggc ggtccgcggc gacaagcggc
     6661 tggtgaagat cttccagatc atccgcgagg acgagcccga ccattgggcg ccctacgagg
     6721 gctggctgca agcgcacggc aagcggcgcg acagctggtg ggagcgcgcg atcgacggct
     6781 tcatccactc cgagctgctg ctcgtgaagc tgccgatcct gttcctgacc ccgcgccttc
     6841 gccggcggag cgagtgggcc gacgctcacg aacgcgtgcc gccggatggc tatcccgagc
     6901 tgaagcaggc gacttgagcg cactggcccg gcgcctgttc tcgcgcaacg cggcggtgct
     6961 gctggcgcgc aacaccgtgg tcagctgcct ggtgttcgtg atcggcctgg cgctgctgtg
     7021 ggcgctcgtc gagctgtggg acgtcgacaa gctggtcgcc gccgcggccg ggttcctggt
     7081 cagcaattcg atccattatg tgttcggccg caagtggatc taccgcggga ccgagcgcgc
     7141 ggtggcgtcg ggctacgcgt atttcctggt caacgccggc atcggcctgg tggtgacggt
     7201 caccttgttc gccgccttcc tgcgctggac gtcgctcaat tatctggtcg cacgggtcgt
     7261 ggtgtcggtc ttcgccggcc tcgccatgtt cctgctcaac gccatgctca acttcaagcg
     7321 cctgtaggcc ggcatgcgcg tcgcgctctt ttccggcaat tacaattacc tccgcgaggg
     7381 cgcgaaccag gcgctcaacc ggctggcccg cgatctcgag gcggacggcc acgaagtgcg
     7441 cgcttattcg ccggtcaccg accgccccgc gttcgagccc gccggaaccc tggtgccggt
     7501 gccgtcgatc ccgctgccgg ttcgaagcga gttccggctg tcgctcgggc tgccgcgggc
     7561 ggtgcgcgag gacgtgcggc gcttcgcacc cgacatcgtc catttgtcga cgcccgacat
     7621 cctcggaacg cgcgcccaga ccttcgccag gcggctaggc attccgaccg tcgccagcct
     7681 ccacacccgg ttcgaggcct atctcgatta ttacggcctc ggctgggccg ggccattggt
     7741 cgaggcgcac ctgcgccgtt tctaccggcg ctgcgatcat gtgctggcgc cgacaccggc
     7801 gctggtcgca gcgatgaagg ccatgcgcgg cgacgatcag gtcagcctgt ggagccgcgg
     7861 cgtcgaccgg gccctgttca acccggcgcg ccgcgacccg gagtggcgcc gatcgaacgg
     7921 ctggtccgac gaggatgtgg tcgtcctgtt cttcggcagg ctggtcgccg aaaaaggcat
     7981 cgaccgctac gtgtcgactc tgcggcagtt acagcgccga agcacgggca tccgcgcgct
     8041 ggcggtcggc gccgggccgg ccgcaggcgc cttcgacgcg cttccgggag cggtcctcac
     8101 cggccatctc gacggcgcgg agctggtccg ggccgtcgcc agcgccgaca tcctgctgca
     8161 cccgagcacg accgaaacct tcggcaatgt cgtcctcgag gcgatggcgt cgggactcgc
     8221 ggtcgtgtgc gcccggaccg acagcgccga ggcgctggtc gagcacgggc gaacggggat
     8281 cttgtgcgcg ccggacgatt gcgaaggcta tcggcaggcg atcctggcgc tggccgaaga
     8341 cccgcggcgc cgcagcgatc tcggcgaggc ggccaggcgc tcgagcgaag ccttttcgtg
     8401 gagcaatgcc tcgcgcagcg tcgcccaggc ctatcgggat gtgctgggcc gtcgccgccg
     8461 gcgctaggaa tttcgagcga ggcggttttt ctaactctgg tgaaggttcc aatcgcaagt
     8521 tcggcgtaac gattggttat ggatcaattc cagtccgacg cagaatattt cgctcggcgc
     8581 tccaacgagg agcgagccgc ggccgagcgc gcgacgcatc cccgcgccag gcagagccac
     8641 atcgaccttg ccgaccgctt tgccgaagcg gctcgcgcca ttgccgaagt ctcgacgctc
     8701 tgggaagccg acatcggcca ggagcggccc gtcgcggttt cagcgcgcca gcccgaattc
     8761 cggatcctgc cgtaaagccg aagcccgtca gccggcgagg cgcttggcgc tgagcccgat
     8821 caccatctgg ttggcgcgct cgtaggacag cgggcggccg aacagatacc cctggatggt
     8881 gtcgcagccg aggtcgcgca tgcgctcgaa atcctcggcc gtctcgacgc cctcggcggt
     8941 gaccgacatc cggaagctct tggcgagctg gacgatcgac tggatgatcg cgacattttc
     9001 cggccgggtg ccggcttcgc ggacgaagct gccgtcgatc ttcaatttgt ggaacaccgc
     9061 cttgttcaga tagccgatcg acgaatagcc ggtgccgaag tcgtcgagcg cgatgccgac
     9121 gccgagctgg cggagtcgct tgaggacgtc gagggtcgag ccgttgtcgc cgaggaacac
     9181 gccctcggtg acctcgagct cgatccggtt gccgggcacc ttgtggcggg ccagcgcctc
     9241 gctgacggtg ttgggcagag cggccagcgc gatctgcttc gggctgatgt tgagggcgac
     9301 ggtgatcggc tccggccagg tcgcggccgc tcggcaggcc tcgtcgatca cccactcgcc
     9361 gatcaccggc atcagcccgg cctcctcggc gaccgggatg aagacgttgg gcggcaccag
     9421 cccgcgctgc gggtggttcc agcggatcag cgcctcgaag ccgatcaatt tctggtcctt
     9481 ggcgttgacc agcggctgga aggcgaggtg gaactgcttg ccggcgatcg ccgcccggag
     9541 gtcggtctcg aggcggaccc ggtcctcctg ctccgactgc agctcggacg agaaatagcg
     9601 ggcgatgccg cggccggcgc ccttggcctc atagagggcg aggtcggcct tgaggatcag
     9661 gtcgtcgacc gtcgctccgt cgatcgggcc gaacgcgcag ccgatcgaga ccccgattcg
     9721 gatctcggtc tggtcgatca tgtagggctc ggcgatcgcc ttgatgatgc ggtcggccat
     9781 ggcctcgaca ttgcggcggc tctgcgcgtc gatgacgacg atcgcgaact cgtcgccgcc
     9841 catccggccg acgtggccgc cgctgccgac ctcgtcgacc agccgcttgg cgaccgcgcg
     9901 cagcaccgcg tcgcccttgg ggtggccgaa ggtgtcgttg accggcttga agccgtcgag
     9961 gtcgaggaac aggatcgcgc acgggacatt gctgacggtc gccgctcgga gcgcgtcgcc
    10021 gagcagctgg cggacccgtc cgcggttggg caggccggac agcacgtcga cgttggcgag
    10081 atgggtcagc cgctcctggg tctggcggat ctcggtgatg tcggagccga ccccgcggaa
    10141 gccctcgaag cggccggcgg tgtcgatgat cgggtcgccg gcgatcgaga tccagcgctc
    10201 gccgcgggcg gtcttcagct ccatctcgag cgagttgaac ggctgccgct cgagcagcag
    10261 ccggccgagc tcggcattgc cgccgagcag cgagggcagc gaatggccga gcagctggct
    10321 cgccgggcgg ccgagcaagg cggtcatgcg cgacgagatg taggtgactc gattctccga
    10381 atcgacctgc cacagccagc cgactccgcg ctgctcatat tcctggagca gcaggctggc
    10441 gctttcgctc tgcgagccga agtcggcgtt ggtcttcagc tggtggaacg cccagcgggt
    10501 gaccgcgagc actccgaaaa tcgcgacccc gagcgtgaac agaatcgaca gcatgtgcgg
    10561 gaagggaacg ctgttgcggc cgaagagcag ggcgacgctg accccggcgg tgaaggccga
    10621 cagccaggcg gtgacgcacg gcgggacgac gaccagcccg agcgctccga tgccgagccc
    10681 ggccgccagc gaggcggcga tcacctgagt gcccggctgc agggacggga acatggccag
    10741 cggcaggctg agccacagca aggcgcgccc ggcgacatcg ccgaccatca gccattgcgg
    10801 aacctggcgg cccgagcggc cgacatgggt gatcgcatgg gtccgggcga gctgcatcgc
    10861 cgccaggttg gcgatcgcca ccgtcccgca ccacggcagc agccaggtga cgtcgacctc
    10921 cccccacagg atcgccgaca tcatcagcgc ggcgacgacg ttggcggccg cgaagaacgg
    10981 cgccagctgc gcgcgcgaga tcaactgcac gtccttcagc ggggagcttt cgccgtcgcc
    11041 ctcgcggctg gtcaggccga gcgattcccc gcccggaagg cgtgctgaga tcatgcgttt
    11101 atagcgatct ggggcttcgt acatccgcct gccgttccac gtcggcccaa gcgggtaacc
    11161 caagagcgtt taagtgcggt taaggggtca accgaaaaat cagccgccgc cgagctttag
    11221 cgcgtcgagc tcgcgggtga cgatcgggtg gtagagcggc cgcccgaggg ggtagatccg
    11281 ggccgcgatc ggccggcccc aggccgaatc ctcggccagc gccttgacca gcgggcggac
    11341 gaacttgcgc cgcccctggg tcgtcaggaa ttgctcgagc gacggcaccg ccggctcgta
    11401 gcggttgccg accgccagct cgagccaggc gaagcgcact tccaggttgc gcacggcgtt
    11461 gaggccgaac gcccggttga gcgcgtcgag ccgcgcggtg gggagcttgc gcggcagcct
    11521 gttgaggaag cgcagccgct cgtcggtcgt ccagcgcgac caggcttcgg tgcccggagc
    11581 gccgccggcg gcaaaggcgg cgaccgcccg gtcggcctcg gcgaacgccc gcgggtcggg
    11641 tttcaccatg ttgggaggca ggcccggctg gtacacccag cggtcgagca tcaacttccg
    11701 ctcgagtgcc tggtcgccct tgatcagatg ctggcgaagg tcggcgagga agatcgccga
    11761 ggtcaccggc tggaaccggt ggcgctcgaa ccagcccttc aaataagcgt cgaaccgatc
    11821 gcggccgacc gccgcctcga tcgtgcgaag gaacgccgcg cccttttcgt aggcgatctc
    11881 cgtcaggccg tcgtccgggt tgcggccctt gaggtcgata tgcagccggg tgtccggagc
    11941 cgacgggccg cccagcgttt cgatctcctc gttcaaggaa tccactccga gcgcgatctg
    12001 ctgcgcggcg accttgggcc cgtagatcgc ttcgacgatg cgggtcgtcg cgtaggtcgt
    12061 catgccctcg ttgagccaga agtcggccca ggtcgcgttg gtcgccaaat tgcccgacca
    12121 gctgtgcgcg agctcgtggg cgatcaggct gacgaggctc ttgtcgccgg cgatgaaggt
    12181 cggggtcagg aaggtgaggt tgggattctc catgccgccg aacgggaagg acggcggcag
    12241 cacgagcatg tcataacgct cccacagata gggcccgtag agcttctcgg ccgagtcgat
    12301 catcttctcg gtgtcggcga gctcggcggc ggccgaatcg agcatcgccg gctcggtcca
    12361 cacgcccgag cgcgggccga gcggcttgaa ggcgagatcg ccgaccgcga tcgcgatcaa
    12421 atagggcgga acgctgttgc gcatccggaa ttcgaacacg ctctcgcccc cttgggtgat
    12481 cggctcggcg gagcgcggcg cgctcatcac cgcggtcagg ccggcaggca cgcggatcgt
    12541 cgcttcccag ctctgccgga tgccgggcga atcctgggtc gggatccagc tgcggttgag
    12601 gatcgcctgt ccctgactga acagataggg gcgcttcttg ccggccgtct gctgcggggt
    12661 cagccactgc aacgcctcgg cgtcgggcgc gctccggtag cggatcacca gccgctttgt
    12721 gtcggggcgg agcgcgacgg ccagcggagc gccgagattt gcgtccgcag cgccgacctt
    12781 ccagcccagc ggctcgccct tgccgtcggt gatggcctgg atctcgagcc ccttgtcgtc
    12841 gaggacgatc tccttcgcgc cgggccggcg gtcgaggtcg agggtcgccg tgccgccgat
    12901 tcgcttgccg tcaaaatcga ccgcgaggtc gagcgcgacg tgatggaccc gcgcttcctg
    12961 cgggcgagca tggctgtgga tatccctcgc gtcgggggtg gccaggatcg gcgcgatcgc
    13021 ggtcgcggtt tcgctcgcgg ccggctgaac ggcattgtcc tgttcggaga cccggcacgc
    13081 ggccgtcaga atcattgcgg ctagcagcgc cgcccgggac cagaatttca tcgaatgctt
    13141 cctcagccgg actcggcgat gccaacgtag agggagcgtt tcggtgccgc gaaaacgcgc
    13201 cggaccatcg gctcgaagaa gtcgagcgga gcgctctcgt agtcagggtc gaacgccgcc
    13261 tggtcgtagg tttcgcagaa gcgggcggtg tcctcgaaat aggggtggcc gcggaacgct
    13321 tcgcgcatgt cgcggtcgag gccgatgtag tggaagaaat aatagccctg gaagatgccg
    13381 tgcttctcgg cgatccagtg cagccggtcg tcgacgaagg gcttgaggat cgccgccgcg
    13441 atgtccgggt ggttgtacga gcccagcgtg tcgccgatgt cgtggatcag cgccatgacc
    13501 acataatcct cggcctcgcc ggcgcggtgg gcgcgggtcg cggtctgcag cgaatgctgc
    13561 agccggtcga ccggaaaacc gccgaaatcg ccgtcgagca gccgcagatg ggcgagcacc
    13621 cggtcgggca ggcccgcggc ataggccatg aactgggtgg cgatcaccgc ccagtcctgc
    13681 tgggtgccct cgtccatgct gcggaaggcg gcgcgcggct gcggctcgtt cactggctct
    13741 ctcctcgccc gaaggactgc agcgaacccg cgcttctcgc aatgaggaaa tttcccgcct
    13801 atatccagcg cgatggcgac cgacagcgcg gcactggaac ggcagcagcg gccggcgagg
    13861 cgggcggccc gtcccggcgc gccccggatc cagtcgccag cggcgccgat cgccgagggc
    13921 atcggcctcg accagccgtt cttcgccgac aagaaccggg cattctggat cctgcagtcg
    13981 atcggctggt cgggctattt catccttcgg accctgtcgg gattcgccaa ctcgatgggc
    14041 tggatgtgga tcgtccacac cctgctcctg accgcgaccg gctattcgct gaccctgctc
    14101 ctggcgaccc tgttccgccg gctgatcgcg atgcgggcgc tgctgacggt ggcgctgtcg
    14161 ctggccgcgg tgatgatcgc cgcgagcgcc ttctcgatca tcgagacgtg gagctacgcg
    14221 accttcctca agcccaacgc ccggccggtc ggcttcgagt atctcggcgc gatcctgctc
    14281 aatttctcgc tgctcgccgc ctgggccgcg ctctattacg ggatcaacta cttccttctg
    14341 ctcgaggagc agatcgacca gcgcgagcgg ctcgagagcc aggcctcgac cgcgcagctg
    14401 gcgatgctcc gctaccagct caacccgcat ttcctgttca acacgctgaa ctcgatctcg
    14461 accctggtgc tgctcaagca gaccgagcgc gccaatgcga tgctcgcccg gctgtcctcg
    14521 ttcctgcgct acaccctggt caacgagccg accgccaagg tcactctggc gcaggaggtc
    14581 gagactctga agctctacct cgagatcgag aagatgcgct tcgaggaccg gctgcgcccg
    14641 catttcaaga tcgaatcgga gaccatcggc gcgcgcctgc cgtcgctgct gcttcagccg
    14701 ctgatcgaga atgcgatcaa atatgcggtg acggcgagcg agagcggggc cgacatctgg
    14761 atcaccgccc accgccaggg ggagtcggtg cggatcgagg tcgccgacaa tggcgacggc
    14821 gagggcgccg ccctggcggc cagcccgtcg accggagtcg ggctggcgaa cattcgggac
    14881 cgtctggcgc aggcctatgg cgccgcgcac gcttttacga cgagaaagaa cgaacgcggg
    14941 gggttcagcg ttatcatcga gattccattc gagagcggag acagggattg atgaccatcc
    15001 ggaccgtcct ggtcgacgac gagccgcttg cgacccaggg gctgcagctc cggctgcagg
    15061 cgcacgagga tgtcgaggtg gtcgccaccg ccgccaacgg ccgcgaggcg atccgggcga
    15121 tcaagaccca caagccggac ctcgtcttcc tcgacatcca gatgcccggg ttcgacggct
    15181 tctcggtcat ccaggggctg atggaggtcg agccgccctt gttcgtgttc gtcaccgcct
    15241 acggcgacca tgcgctgagg gcgttcgaat cccaggcggt cgactatctg atgaagccgg
    15301 tcgacgagga ccgcctggcc gcgaccctcg accgggtgcg ccagcggctg gtcgagaagc
    15361 gcggcgccga ggaggccgag cggctgaagg aggtgctggt cgagcatgcg cccgaggcgg
    15421 cggaatcgct cgccgaggag accgccggcg acgccccggc cgccaaccgc ttcgagcgga
    15481 tgatcaacat caaggaccag gggcagatct tccgcgtcga cgtcgacacg atcgagcgga
    15541 tcgatgcggc cggcgactat atgtgcatcc agaccggcga caacacgctg atcctgcgcg
    15601 agacgatgaa ggatttggaa aagcgcctcg acccgcgccg gttccagcgc gtccaccgct
    15661 cgaccatcgt caacctcgac ctcgtccggc aggtcaagcc gcacaccaac ggcgagtgct
    15721 tcctggtgct ggattcgggc gctcaggtga aggtcagccg ttcctaccgc gacgtggtgg
    15781 cgcggttcgt gcactgatct tcagggggga gcgcatcgcc gcggaccgga aggtgagccg
    15841 cgatccctcc atgacctgcg cgctccggcg gccacgactt gctccattta cgaccaaatg
    15901 ttgagggcct ctcaatcact tcggtgacac ctccgcccgg ttctgctcgg agcaatcgag
    15961 gacggcatga agggcctggt gttcacgaca ttctaccgcc attgcgaaag ccgctacggc
    16021 gccgactttc tcgatgatct gatcgaggaa gcggcgctgc ccaaccaggg cgcctatacc
    16081 agcgtcggca gctacccgtt cgaggaaatg gttgcgctgg tgacggcgct ggcgagcaag
    16141 accggaacgc cgatgccggt gatcctccgg gaattcggcg agttctgctt cctccagtgg
    16201 gtccgctatg tccccgccca tttcgacgga aagcagctgt tcgacatcat ggccggaatc
    16261 gacggattcc acgagaatga agtccgcaag ctgtacccgg acgccgagct gccttcgttc
    16321 aaggtggaaa gccgcgacgg cgaccggatg atcctgcgct accacagctg caagccgctg
    16381 gcggacctgg cggtcggagt catccacggc gcggcgaatt acctcggcga gacggtcgaa
    16441 gtgcaccacc agcccatggc cgagccacgg gggccgtatg tccgcatcga aatcaatcgc
    16501 gtctcttgag gttgcgggcc gccggggaga cgatttccgg ggcggcgtgg acgaccgcct
    16561 gaggcggctc gagctcaggg tcgcccgcga gcacgccgcg cggcgggagg cggagagcat
    16621 cctggaaagc aagagcctgg agctgttcgc ggccaaccag aggctcctcc agctcaacat
    16681 ggagctggaa gagcgtgtcg cgcgccggac ggaagagctc gaccgggcgc gccgctcggc
    16741 gctccagctg gtcgagaacg accatctgac gaagattccg agccgcttcc gcttttcccg
    16801 ggccctccac gaatcgctcg aacggacgct gcaaaccggc ggcagcatcg gcctgctgct
    16861 gatcgacatc gatgatttca agctggtcaa cgattgctac ggccacggcc acggcgacct
    16921 cctgctcaag gagatcgcgg cccggctgcg gtcgatcgtc cggcgccacg aactggtcgc
    16981 gaggctcggc ggcgacgagt tcgcagtgat cctagaaggg tccgccgctg cctcgctcga
    17041 acggggcgca cggcgtttcc aggcagcgtt cgtcgagccc gtgacggttg gcggggtgac
    17101 gttgaagatc gcgggctcga tgggcctcgc gacgagcccc gaggacgcga ccaccagcgc
    17161 cgacctccag cgcttcgccg acctcgcctt gtaccaggtg aaggcgaggg gcggcggcgg
    17221 cttcgcgcag ttcgagaagg ggctggtcga ttcctacgac cgccgccgcc gcctggaggc
    17281 ggaattccga ggggcggtcg caaatgacgc aatccatctg tggttccagc cgatcgtcga
    17341 aatccgcacc ggcgaaacca tcggggtcga agcgctggcc cggtggcagg acagccagca
    17401 tgacgcaatc ggccccgatt tcttcattcc gctggccgaa cagtgcgggc tgatccgcga
    17461 ggtcgggcgg catcttctcg gcaaggcgct tgcagccgcg aagccgtggc tcgacgacgg
    17521 gctgatccgc aagctgacgt tcaacatctc gccgctcgag ctgctcgatg acgggttcgc
    17581 cgacgagatc ctcgaatcgc tggcggccgc gcaggtcgag cctgcaaagc tggcgctcga
    17641 gatcaccgag ggcgtgctga tcaagaatct aagcctcgcc aaggcggtga tggcgcgcct
    17701 gcgcagggcg ggcgtcaagt tcgcgctcga cgatttcggc tgcggctaca ccaacctgtc
    17761 ggcgctgacc aacttgccga tctcgatcct caagatcgac cgcaccctgc tcaggaacgt
    17821 cgagcatgac gaaagtgcgc gggtgatcat cagcaatgtc gtcaacctgt gccgcgacct
    17881 tggcatccgc tcggtgtgcg aaggggcgga aacggccgcg cagctcgcct ttctcgactt
    17941 gatcggctgc aattcggtcc agggctatgt ctgcgcgagg ccggcgtccg cacaggatac
    18001 cgagattttc ctgcgccgcg cggtgcaacg ccggcgcgat cccgggctgc gcccccgggg
    18061 cccgaagagc gcggtcgcct aggcccggcc ggtccggccg cggacgggcg agccgattgc
    18121 cgagcgcgca cggcggctgc tagaccggcc cgatgaccaa gacccgctgg ccaggagccg
    18181 ccgc
//
LOCUS       DASTZO010000247         6543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_255917,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000247 DASTZO010000000
VERSION     DASTZO010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255917"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="VFU20_06905"
     CDS             complement(<1..377)
                     /locus_tag="VFU20_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5286221.1"
                     /translation="MKAERISENRTRSLLLLDADPDERRLVSAIAGRAGWSVIGSACG
                     ETAVGLLQGPHGREVQAALIGNWDSDTGPELIAELRSCRENLPIIVVADGDSVAVAVE
                     AMRAGATDFLSRPVVQERLIEALA"
     gene            494..643
                     /locus_tag="VFU20_06910"
     CDS             494..643
                     /locus_tag="VFU20_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286222.1"
                     /translation="MAEEEPILTNPPTQEVAHHVRDYTRFVHLLKWGAIISLVTALFV
                     MMIIS"
     gene            676..1800
                     /locus_tag="VFU20_06915"
     CDS             676..1800
                     /locus_tag="VFU20_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU5286223.1"
                     /translation="MKIAILKERAPGETRCAAIPETVKKFTALGADIAVESGAGDNAL
                     ISDEEFEAVGAKVGSRAEVLKGAGAILCVSGPEPAELAGAEPGALLVGGLDPAGRREA
                     IDGYAAAGLEALAMEWMPRISRAQSMDILSSQSNLAGYKAVIEAAALYGRALPMMMTA
                     AGTVSPAKVFVMGVGVAGLQAIATARRMGAQVSATDVRSATREQILSLGAKPIFVETV
                     AGIKGEGAGGYATEMSDEYQKAQAELVSSHIARQDIVITTALIPGKPAPRLISDAQLA
                     TMRGGSIIVDLAADAGGNVEGCVAGEQVRRHGVTIVGAGNLARSLSADASALFARNVY
                     NFLSAFWDREAKRPALPDDDELVQGIRLTRAGKVVHERLS"
     gene            complement(1810..2280)
                     /locus_tag="VFU20_06920"
     CDS             complement(1810..2280)
                     /locus_tag="VFU20_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HEU5286224.1"
                     /translation="MSLESVRRWLAAHAPDVELIEVAETTATVDTAARALGVEPARIA
                     KTLAVRAGEAVFLLVARGDARLDNAKCKAEFGARPRMLGPDETRALTGHPVGGVCPFG
                     LATPLPVYLDVSLKPFDRVYPAAGSLNTSVRLTPQRLYELVGSRWVDLCRLPEP"
     gene            complement(2277..2699)
                     /locus_tag="VFU20_06925"
     CDS             complement(2277..2699)
                     /locus_tag="VFU20_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008603383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEU5286225.1"
                     /translation="MVRQSFSTLDVRAPAQGLHEISDEVGRWVAASGISDGLLTLFCR
                     HSSASLLIQENAAPAARRDLEAYFARIAPEDPSAYAHNAEGPDDMPAHLRSALTQTQL
                     AIPVQAGRMLLGTWQGIYLFEHRREPPPRQIVLQLAGE"
     gene            complement(3129..5429)
                     /locus_tag="VFU20_06930"
     CDS             complement(3129..5429)
                     /locus_tag="VFU20_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5286226.1"
                     /translation="MPSRRKHKSEPSPSRLLLWVAIAGLIFGLIGFGELPEDVLRAGR
                     NSLHPHKASGDIVLVQIDDRSLRELGRWPWPRRFHAQLTDKLTAAGAQRIFFDVMFEN
                     RSNAADDAMLAAAIERSGRVTLPVRLRSGPYGSKPNSAQLDSRPLDLFSRNARLGTIS
                     VRYNYQNAVWNVPYAVKVNDRLTPSFAASLARSKTLTEAEFTPDYSLDIGTIPTISAA
                     AVLKGSVRSGLVRGKDVVIGTNSEAAGDQYFIPGVGKMGGAYVQIVGAETLKAGSPVH
                     LGWIPAFLLAVAIAAFAARQKNPARQNLICGGGAAALLFAPALFEANLMFVDVTPALF
                     VLLTYGSVTAWRGFRVRGLVNPVSGLPNLNALRLNRAGRSQALLVARILNYSEIVATL
                     PGDRERQLIEQIVGRLSVGSSERTFYHGDGGILAWFEEPQLPFGNHIDALYSLFRNPV
                     RVGGLAIDLAIAFGVEIGSGRSLGNRLGSAMVAAEEAGHAGLKWKFHDPDTLQDASWK
                     LSILSQLDSAIDKGEVWVAYQPKLDLASRQIIGAEALARWTHPEKGPIAASEFVAAAE
                     QHDRIGKLTDFVLDKAVAAGASITRKGRDFNIAVNLSARLLTDRNFTLRLSALLARHG
                     LEPRHLTLELTETAAIIGSGEAVDMLARLREIGVNIAIDDYGTGLSTLDYLKKIPAGE
                     IKIDQSFVKAMCDNRSDRVMVQSTIVLAHSLGRKVVAEGVEQRKILDALIEMKCDVAQ
                     GYIIGRPMSLESLVKRIATDRKLSVA"
     gene            5601..5891
                     /locus_tag="VFU20_06935"
     CDS             5601..5891
                     /locus_tag="VFU20_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha"
                     /protein_id="HEU5286227.1"
                     /translation="MDFIAILSIFVLACFVGYFVVWSVTPALHTPLMSVTNAISSVIV
                     VGALIAAAAAGSPTAKWLGLVAVALASVNIFGGFAVTQRMLSMYRKKERRDA"
     gene            5884..>6543
                     /locus_tag="VFU20_06940"
     CDS             5884..>6543
                     /locus_tag="VFU20_06940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU5286228.1"
                     /translation="MHEAGAGAPPAWVLIAYLVAGVCFILALRGLSSPESSRRGNRLG
                     MIGMAIAVVTTLAIHEIASVIEIAAAILIGGAIGLVTARKIQMTAMPQLVAAFHSLVG
                     MAAVLVGAAAFLNPTAFGITDAAGDILQVSRVEMGLGVAIGAITFSGSVIAFLKLNGN
                     MSGAPILLPGRHVINLGTLAAIVGLIAWFTQDQAPWVFWTLTALAFAIGFLLIIPIGG
                     AD"
BASE COUNT         1038 a         2153 c         2325 g         1027 t
ORIGIN      
        1 gccagcgctt cgatcagccg ctcctggacc accggccggc tgaggaaatc ggttgctccg
       61 gcgcgcatcg cctcgaccgc gacggcgacc gaatcgccgt cggcgacgac gatgatcggc
      121 aaattctcgc ggcacgagcg cagctcggcg atcaactcgg ggccggtgtc cgaatcccaa
      181 ttgccgatca gcgccgcctg gacctcgcgt ccgtgcgggc cctgcagcag cccgaccgcg
      241 gtctcgccgc aggcggagcc gatcacgctc cagccggcgc ggccggcgat cgccgacacc
      301 agccgccgct cgtcggggtc ggcgtccagc agcaatagcg accgcgtacg gttctcgctg
      361 atccgttccg ctttcatggc cgcacttgta gcttcacagg agtaaaggcg cgcttaaagg
      421 cttgggcgcg gtcggcccgc ttgttatggg cggtccggca gcaccagcag aaccagcaga
      481 accggggagc agcatggcgg aagaagagcc gatcctgacc aaccctccga cccaggaggt
      541 cgcccaccac gtgcgcgact acacgcgctt cgtgcacttg ttgaaatggg gcgcgatcat
      601 ctcgctggtc accgccctct tcgtcatgat gatcatcagc taggccgcat tccagctgat
      661 gaaatctgcc caggaatgaa gatcgcgatc ctcaaggagc gcgcgccggg cgagacccgc
      721 tgcgcggcga ttcccgagac ggtgaagaag ttcacggcgc tgggagcgga catcgcagtg
      781 gaatccggcg ccggcgacaa cgcgttgata tccgacgagg aattcgaagc ggtgggggcg
      841 aaggtcggct cgcgggccga ggtgctgaaa ggagccggcg caatcctgtg cgtaagcggc
      901 cccgaaccgg ccgagctggc cggcgcggag ccgggtgcgc tgctggtcgg cgggctcgac
      961 ccggccgggc ggcgcgaggc gatcgacgga tatgccgccg cggggctcga ggcgctggcg
     1021 atggagtgga tgccgcgaat ctcgcgcgcc cagtcgatgg acatcttgtc gtcgcagtcg
     1081 aacctcgccg gctacaaggc ggtgatcgag gccgccgcgc tctacggacg ggcgctgccg
     1141 atgatgatga cggccgcggg cacggtcagc ccggccaagg tgttcgtcat gggggtcggg
     1201 gtcgccgggc tgcaggcgat cgccaccgcc cgccggatgg gcgcccaggt cagcgcgacc
     1261 gacgtccgct cggcgacccg cgagcagatc ctgtcgctcg gcgccaagcc gatcttcgtc
     1321 gagacggtcg ccggcatcaa gggcgagggc gctggcggct atgcgacgga aatgtcggat
     1381 gaatatcaaa aggcgcaggc cgagctggtg tcgagccata tcgccaggca ggacatcgtc
     1441 atcaccaccg ccctgatccc cggcaagccg gctccgcggc tgatcagcga tgcgcagctg
     1501 gcgacgatgc ggggcgggag catcatcgtc gatttggcgg cggacgccgg cggcaatgtc
     1561 gagggctgcg tcgcgggcga gcaggtgcgg cgtcatggcg tcaccatcgt cggggccggc
     1621 aacctcgcgc gcagcctttc ggccgacgcg tcggcgctgt tcgcgcgcaa cgtctacaat
     1681 ttcctcagcg ccttctggga cagggaggcg aagcggccgg cgcttcccga cgacgacgag
     1741 ctggtccagg gcatccgcct gacccgcgcc ggcaaggtcg tccacgaacg gctgagttag
     1801 gcggctgagt tagggttccg gcagccggca gaggtcgacc cagcggctgc cgaccagctc
     1861 gtacaggcgt tgtggagtga gccggacgga cgtgttgagc gagccggcgg ccggatagac
     1921 ccggtcgaac ggcttcaggc tgacatcgag atagacaggc agcggcgtcg cgaggccgaa
     1981 cgggcagacg ccgccgaccg ggtggccggt cagcgcccgg gtctcgtcgg gtccgagcat
     2041 ccgcgggcgg gcgccgaact cggccttgca cttggcgttg tcgagccggg catcgccgcg
     2101 cgcgacgagc aggaacacgg cctcgccggc gcgcaccgca agcgtcttgg cgatccgcgc
     2161 gggctccacc cccagcgctc gggcggcggt gtcgaccgtc gcggtggttt cggcgacctc
     2221 gatcagctcg acgtcgggcg cgtgcgcggc gagccatcgg cggacggatt cgagactcat
     2281 tcgcccgcca attggagcac gatctggcgc ggcggcggct cgcggcggtg ctcgaacaga
     2341 tagatgccct gccaggtgcc gagcagcatc cggccagcct ggaccgggat cgccagctgg
     2401 gtctgggtca gcgccgagcg caggtgcgcc ggcatgtcgt ccggcccctc ggcgttgtgg
     2461 gcgtaggcgg acgggtcctc gggcgcgatc cgggcgaaat aggcctcgag gtcgcggcgg
     2521 gcggccgggg cggcattctc ctggatcagc agcgaggccg agctgtggcg gcagaacagg
     2581 gtcagcaggc cgtcgctgat gccgctggcc gcgacccagc ggccgacctc gtcgctgatc
     2641 tcgtgaagcc cctgggcggg agcgcggacg tcgagggtgg agaaggattg ccggaccatg
     2701 gcccgccgct agggcagcgg cggcgcgggc gccagcccgg cccgaatcgc ccgaatcggg
     2761 acggcgcgat tcgcgccgat tcggcccttt tcaatgcttg ggggcgggcc ggccggccgc
     2821 aaaggacaag gcccggtcga gagccgcctg ggggccggga gccgaaggtg caccaaaagc
     2881 gcccgcaaaa tcgcaggcgc ttgattaggc gcttgaatca aaagtcgaaa aacgcttcgg
     2941 ccgaagccga acgcaacctt tacccgctgc taccggtgtt gctccagcca ccgccgagaa
     3001 gccaggtcca gaagccgcgg ctggcttggg ttttcttggt catcgaacgt cccctgtggt
     3061 gccaaactgc acatcggcag tgaggggaca ttaaccctaa tcgatatggt tagcaaacga
     3121 ttaactaatc aggcgacgct caacttgcgg tcggtggcga tcctcttgac caggctctcc
     3181 aggctcatcg ggcggccgat gatatacccc tgggcgacgt cgcatttcat ttcgatcagg
     3241 gcatcgagga tcttgcgctg ctcgactcct tcggcgacca ccttccggcc cagcgaatgg
     3301 gcgaggacga tggtcgactg gaccatcacc cggtcgctcc ggttgtcgca catcgccttg
     3361 acgaaactct ggtcgatctt gatctcgccg gccgggatct tcttgagata atcgagcgtc
     3421 gaaaggccgg tcccgtaatc gtcgatggcg atgttgaccc cgatctcgcg gagcctcgcc
     3481 agcatgtcca ccgcctcgcc gctgccgatg atcgccgcgg tctcggtcag ctcaagggtc
     3541 aggtgccgcg gctcgagccc gtggcgggca agcagggccg acagccgcag ggtgaagttg
     3601 cggtcggtga gcagccgcgc cgacaaattg accgcgatgt tgaagtcgcg gcccttgcgg
     3661 gtgatcgagg cgcccgccgc gaccgccttg tcgaggacga agtcggtgag cttgccgatg
     3721 cggtcgtgct gctcggccgc ggcgacgaac tcggaggcgg cgatcggccc cttctccgga
     3781 tgggtccagc gggcgagcgc ttcggcgccg atgatctggc gggaagcgag atcgagcttg
     3841 ggctggtagg cgacccacac ctcgcccttg tcgatggcgc tgtcgagctg gctgaggatc
     3901 gacagcttcc aggacgcgtc ctggagggtg tcggggtcgt ggaatttcca cttgaggccg
     3961 gcgtggcccg cttcctcggc cgcgaccatg gcgctgccga ggcggttgcc gagcgagcgg
     4021 ccgctgccga tctcgacgcc gaaggcgatc gcgaggtcga tcgccagccc gccgacccgg
     4081 accgggttgc ggaacagcga gtagagcgca tcgatatggt tgccgaacgg cagctgcggc
     4141 tcctcgaacc aggcgaggat gccgccgtcg ccgtggtaga aggtgcgctc gctcgagccg
     4201 accgacagcc ggccgacgat ctgctcgatg agctggcgct cgcggtcgcc gggcagggtg
     4261 gcgacgatct ccgaataatt gaggatccgg gcgacgagca gcgcctggct gcggccggcg
     4321 cggttcaggc gcagcgcgtt gaggttgggg aggcccgaca ccgggttgac cagcccgcgg
     4381 acccggaacc cgcgccatgc ggtcaccgat ccgtaggtga ggaggacgaa caaggccgga
     4441 gtgacgtcga cgaacatcag gttcgcctcg aacagcgccg gcgcgaacag cagcgccgcg
     4501 gcgccgccgc cgcagatcag attctgcctg gcggggttct tctgccgggc ggcgaaggcg
     4561 gcgattgcga ccgcgagcag gaacgccggg atccagccga ggtggacggg ggagccggcc
     4621 ttgagggttt cggcgccgac gatctggacg taggcgccgc ccatcttgcc gaccccgggg
     4681 atgaaatact ggtcgccggc cgcctcggag ttggtgccga tgacgacgtc cttgccgcgc
     4741 accaggccgg accggaccga gcccttcaac accgcggccg ccgagatcgt cgggatcgtg
     4801 ccgatatcga gcgaatagtc gggcgtgaat tccgcctccg tcagcgtctt gctgcgggca
     4861 aggcttgccg cgaacgaggg agtcagccgg tcgttgacct tgaccgcata gggcacgttc
     4921 cagacggcgt tctggtaatt gtagcggacg ctgatcgttc cgagccgggc gttgcggctg
     4981 aacaggtcga gcggccggga atcaagctgg gcggaattgg gcttcgaccc gtaggggccc
     5041 gagcgcagcc ggaccggcag ggtcacccgc ccggaccgtt cgatcgccgc cgccagcatc
     5101 gcatcgtcgg ccgcattgct gcggttttcg aacatcacgt cgaagaagat ccgctgggct
     5161 ccggccgcgg tgagcttgtc ggtcaattgg gcgtggaagc gcctcggcca cggccagcgc
     5221 ccgagctcgc gaagcgagcg gtcgtcgatc tggaccagga cgatgtcgcc gctggccttg
     5281 tgcgggtgga ggctgttgcg gcccgcgcgc agcacatcct ccggaagctc gccaaagccg
     5341 atgagcccga agatcagtcc ggcgatcgcc acccacagca gcagcctgga cggcgacggc
     5401 tcggacttgt gtttcctgcg cgacggcacc gagcgcctgt tcccttccca cttcctcgct
     5461 ccacctgtgc cgcgaaaagg ttatcaaatc ggaaaccgcg tttgggttaa ttgcaccgcg
     5521 cacgccttcg ttcgccgccc actcgacagc attcattcga gccgccacaa agcgccgctg
     5581 gtcagcgtgt cggagggccc atggacttca tcgcgatcct gtcgatcttc gtgctcgcct
     5641 gcttcgtcgg ctacttcgtc gtctggtcgg tgaccccggc gctgcacacg ccactgatga
     5701 gcgtgaccaa cgcgatctcg tcggtgatcg tggtcggggc gctgatcgcc gccgccgcgg
     5761 ccggcagccc gaccgccaaa tggctcgggc tggtcgcggt cgcgctggcc agcgtgaaca
     5821 tcttcggcgg cttcgcggtc acccagcgga tgctgagcat gtaccgcaag aaggagcgcc
     5881 gcgatgcatg aggcgggcgc gggtgcgccg cccgcctggg tgctgatcgc ctatctggtc
     5941 gccggagtct gcttcatcct cgcgctgcgc ggcctgtcga gcccggaaag cagccgccgc
     6001 ggcaaccggc tcgggatgat cggcatggcc atcgcggtcg tcacgacgct cgccatccac
     6061 gagatcgcct cggtgatcga gatcgccgcc gccatcctga tcggcggcgc gatcggcctg
     6121 gtcaccgccc gcaagatcca gatgaccgcg atgccgcagc tggtcgcggc gttccacagc
     6181 ttggtcggga tggccgccgt gctggtcggg gcggcggcgt tcctcaaccc gaccgcgttc
     6241 gggatcaccg acgcggccgg ggacatcctg caagtcagcc gcgtcgagat ggggctcggg
     6301 gtggcgatcg gagcgatcac cttctcgggc tcggtgatcg ccttcctcaa gctgaacggg
     6361 aacatgtcgg gcgcgccgat cctgctgccc ggccgccacg tgatcaacct cggcacgctg
     6421 gcggcgatcg tcgggctgat cgcctggttc acccaggacc aggccccgtg ggtcttctgg
     6481 accctgaccg cgctcgcctt cgcgatcggc ttccttttga tcatcccgat cggcggcgcc
     6541 gac
//
LOCUS       DASTZO010000248         5990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_672509,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000248 DASTZO010000000
VERSION     DASTZO010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672509"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="VFU20_06945"
     CDS             <1..1182
                     /locus_tag="VFU20_06945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007685819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HEU5286229.1"
                     /translation="GYGRCYFSATSAHTCTGDGNAMVLRAGLPLQDMEFVQFHPTGVY
                     GVGVLITEGARGEGGYLTNSEGERFMERYAPSAKDLASRDVVSRSMAMEIREGRGVGE
                     HKDHIFLHLDHIDPKILAERLPGITETAMTFAGVDLTRQPVPVVPTVHYNMGGIPTNY
                     HGEVVTLRDGNPEAVVPGLFAVGEAACVSVHGANRLGSNSLIDLVVFGRAAGLRLAEV
                     VKPGALRDRLPKEAGALAIERLDRFRNADGGSPTAIVRDQMQRTMQADCSVFRTQATL
                     AEGMSKIDQVFARMADVRVTDRSLIWNSDLVETLELDNLLAQAVVTMHCAANRKESRG
                     AHMREDYPERSDGEWMKHTVAWLDGWGGKGGTVRIDYRPVHEFTLTDDIGYIKPKARV
                     Y"
     gene            1182..2033
                     /locus_tag="VFU20_06950"
     CDS             1182..2033
                     /locus_tag="VFU20_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286230.1"
                     /translation="MSPFSGMAGLTKNPLGILAIFLGVLYGIAGMLLGARADALTDWN
                     ETILTLLIFLFPFVSLGVFGWLVSRHHANLYAPSDFRSDSGFLASAKKASQKEIDKKI
                     EQEATVIDGGELAGVSVEGKLIQGPATADTHSKQQASTASDRRAVVRQAESQVLDLLE
                     ADFDKLRKDVKFDTLSGRPVLIDAIGLKDDQFTLVEVITVRLRSTFETRVRDLALNLL
                     SIEPSVRDARKTQVLVCVVWLEGQPNLIDQAIRRAKSIFKRVGLERVAVRQFVLSDDG
                     IVQSLDS"
     gene            2165..3562
                     /gene="creD"
                     /locus_tag="VFU20_06955"
     CDS             2165..3562
                     /gene="creD"
                     /locus_tag="VFU20_06955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HEU5286231.1"
                     /translation="MFLGDRERTPGFKLFLAILIAGALVIPLFSVWLLVYDRQTQSEQ
                     AAASITEGWGGPQAVSGPLLVIPYRATATETVVEGGQSVTRSREVMRELSLAPETVEL
                     TTDVRPERRTRSIYEAVVYDARVAGKARFAFPPDLARHGVAPADIDLGRAELRFGLSD
                     PRGLGADPRVLADGRRLRLQPGGGSGGGRGFFAWIDAAGLTERPLSVDFAYDFRGNAS
                     LSLAPQAGDTRWTLRSAWPHPSFGGSFLPARRSVGDQGFEATYRVGNLALGRSLVSTG
                     DAGASNVVERIAPPSHSMPAPVADSGAPQTAHVSLIQPVDLYSRVNRATKYGFLFIGF
                     TFLALLMFDVIGGIRISPVEYLLMGAALVLFFVLLLAFAEVIGFAAAYVLASAAIAGL
                     NTAYSAAILRSWRRAGLIGGLLVALYAVLYILLNLEAYSLLIGSLLLFAALAGVMYGT
                     RRIDWSARSGEKQPA"
     gene            3618..4478
                     /locus_tag="VFU20_06960"
     CDS             3618..4478
                     /locus_tag="VFU20_06960"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HEU5286232.1"
                     /translation="MSESIPPERIFPTSKVDAQAARHVQSSPQTESEAYKLAFQDTDF
                     LLRPDLRPVRFQLELLKPELLLDEAKIRSMIVFYGSARIPAPDMADALVASATTPEQR
                     AVAERLKAKSHYYTVAYELARLASRTPPDADGWRDFVVCSGGGPAIMEAANRGSVDEG
                     RETIGLNIVLPHEQLPNRYVTPELSFQFHYFALRKMHFLLRARAIAVFPGGYGTFDEM
                     FELLTLIQTGKVRPLPILLFGREFWTKVVDFQALVDEGVISPHDLDLIHWSEDAAEAW
                     DYVQHFYEEE"
     gene            complement(4491..5168)
                     /locus_tag="VFU20_06965"
     CDS             complement(4491..5168)
                     /locus_tag="VFU20_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HEU5286233.1"
                     /translation="MNDRFTTVSGWVLFAGIIALGSSIVTGEVFHAERPETMGYPIEG
                     VELEGAEGEAEKPIAFYLASADAAKGEQVFKKCAACHNAEPGGPNALGPALYGVMGNP
                     VAGHPGFAFSDALKSKGGSWDWEAMSAWLANPKKFAPGTKMTFAGLGNPQDRADVMAF
                     LNSRDNAPLPVPAAPAEAAADAGDGAQKAENEPVLNEQQAAKGGEKNVGGAGAPKVTG
                     PSGVDKN"
     gene            complement(5256..>5990)
                     /locus_tag="VFU20_06970"
     CDS             complement(5256..>5990)
                     /locus_tag="VFU20_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5286234.1"
                     /translation="NLIACAREVAARGGFPSSEAGLRALPGIGDYSAAAIAAIAFGRN
                     AAVVDTNVARVVARLNGLEQPSRREIRVLVEAMTPAGRAGDFAQAMMDLGATVCRPKA
                     PECPTCPLRSGCAARASGTPEAFPQAPARRARPTRHGVAWWIEQDGAVWLVRRPPRGM
                     LGGMAALPGGEWVDSPPPLPSPPLARVRHLFSHFALELAVLRGGKPLGDGWWQPLERL
                     GEAGLPTLYRRAAELVLERSGDRRAA"
BASE COUNT          929 a         2035 c         1984 g         1042 t
ORIGIN      
        1 ggctacgggc gatgctattt ctcagccacc tcggcccaca cctgcaccgg cgacggcaat
       61 gcgatggtgc tccgcgccgg cctgccgctg caggacatgg agttcgtcca gttccacccg
      121 accggggtct acggggtcgg cgtgctgatc accgagggcg cccgaggcga gggcggctat
      181 ctgaccaaca gcgagggaga gcgcttcatg gagcgctacg ccccgtcggc caaggacctc
      241 gcgtcgcgcg acgtcgtctc acgctcgatg gcgatggaga tccgcgaggg ccgcggggtc
      301 ggcgagcaca aggaccatat cttcctccac ctcgaccata tcgacccgaa gatcctcgcc
      361 gagcggctgc ccgggatcac cgagacggcg atgaccttcg ccggcgtcga tttgacccgc
      421 cagccggtcc cggtggtgcc gaccgtccat tacaacatgg gcgggatccc gaccaattat
      481 cacggcgagg tggtgaccct tcgcgacggc aatcccgagg ccgtcgtccc cggcctgttc
      541 gcggtcggcg aggcggcctg cgtcagcgtc cacggcgcca accggctcgg ctccaacagc
      601 ctcatcgacc tggtcgtgtt cggccgcgcc gccgggctcc ggctcgccga ggtggtcaag
      661 cccggagctc tgcgcgatcg gctgcccaag gaagccggcg cgctggccat cgagcggctc
      721 gaccgcttcc gcaacgccga cggcggcagc ccgaccgcca tcgtccgtga ccaaatgcag
      781 cgcacgatgc aggccgattg ttcggtgttc cggacccaag ccacgctcgc cgagggcatg
      841 agcaagatcg accaggtttt cgcccgcatg gccgacgtca gggtcaccga ccgaagcctc
      901 atatggaaca gcgacctcgt cgagactctc gagctcgaca atttgctcgc ccaggcggtg
      961 gtgaccatgc attgcgccgc caaccggaag gaaagccgcg gcgcccacat gcgagaggat
     1021 tatcccgagc gcagcgacgg cgaatggatg aagcacaccg tcgcctggct cgacggctgg
     1081 ggcgggaagg gcggcacagt ccggatcgac taccgaccgg tgcacgagtt cacgctcacc
     1141 gacgacatcg gctacattaa accaaaagcg agggtgtatt gatgtcgccg ttttccggaa
     1201 tggctgggtt gaccaagaat ccattaggaa ttttggcgat ctttttgggc gttctctacg
     1261 ggattgccgg gatgctcctg ggtgctcgag ccgatgccct tactgactgg aatgaaacca
     1321 ttcttacatt attgattttt ctattcccgt tcgtcagcct tggcgttttc ggttggctcg
     1381 tctctcgtca tcacgccaat ttatacgctc cgtcggattt tcggagtgac tccggattct
     1441 tggcttcagc aaaaaaggcg tcgcagaagg aaattgacaa gaaaatagag caagaagcta
     1501 cggttatcga cggaggcgaa ctcgcaggag tttcagtgga gggaaaactg atccaaggcc
     1561 ctgccactgc tgatacgcat tcgaagcaac aggcatcaac ggcttcagat cggcgtgcgg
     1621 ttgtccgcca agccgaatcg caggtattgg atttgctcga ggcggacttc gacaagctcc
     1681 gtaaggatgt gaagttcgac acattaagcg ggcggccagt tttgatcgat gccatcggtc
     1741 taaaggatga tcaatttaca cttgtagagg tgatcacggt gaggcttagg tctacatttg
     1801 agacccgggt gcgtgactta gcgctgaatt tgctttctat tgagccctcg gttcgtgatg
     1861 caagaaaaac tcaggtgctc gtctgcgtcg tgtggcttga gggccagcct aatttgatcg
     1921 atcaagccat tcggcgcgcc aagtccatct ttaagagggt gggactagag cgcgtagcgg
     1981 ttcggcagtt cgtcctgtcc gatgatggga ttgtgcaatc tctggattcc taagcggtcg
     2041 ctcaaagtgc ggcctttttc agcaaatcct cacaagttgc tcatggcgct caccatcggg
     2101 atcggccagg cttgagcttc gatcggccgg atgagccggg ctgccatttt cgggaggacc
     2161 tccagtgttt ctaggcgacc gtgagcgcac tccgggcttc aagctcttcc tcgcgatcct
     2221 gatcgccggc gcgctcgtca tcccgctctt ctcggtgtgg ctgctggtct acgaccgcca
     2281 gacccagtcc gagcaggcgg ccgcctcgat caccgaaggc tggggcgggc cgcaggcggt
     2341 gtccgggccg ctgctggtca tcccctaccg agcgaccgcg accgaaacgg tggtcgaggg
     2401 cggccagagc gtcacccgca gccgcgaggt gatgcgcgaa ctgagcctcg cccccgaaac
     2461 cgtcgagctg acgaccgacg tccgccccga gcgccgcacg cgctcgatct acgaggcggt
     2521 ggtctatgac gcgcgggtcg ccggaaaggc ccgcttcgcc ttcccgccgg acctggcgcg
     2581 gcacggggtc gcgcccgccg acattgatct cggccgggcc gagctgcgct tcggcctcag
     2641 cgacccgcgc ggcctcggcg ccgacccgcg ggtcctcgcc gacggacggc ggctgcggct
     2701 ccagccgggc ggcggcagcg gcggcggccg cggcttcttc gcctggatcg acgcggcggg
     2761 ccttacggag cggccgctca gcgtcgattt cgcctacgac ttccgcggca acgcgtcgct
     2821 gagcctcgcc ccgcaggccg gcgacacgcg ctggaccctg cgctcggcct ggccgcaccc
     2881 gagcttcggc ggctcgttcc ttccggcccg gcgcagcgtc ggcgaccagg gcttcgaggc
     2941 gacctaccgg gtcggcaacc ttgcgctcgg gcggtcgctg gtctcgaccg gcgatgccgg
     3001 cgcgtcgaac gtcgtcgagc gaattgcgcc gccgtcgcac tcgatgccgg cgccggtcgc
     3061 ggattccggc gcgccgcaga ccgcccacgt cagcctgatc cagccggtcg acctctattc
     3121 gcgggtcaac cgggcgacca aatacggctt cctgttcatc ggcttcacct tcctcgcctt
     3181 gctgatgttc gacgttatcg gcggaattcg aatctcgccg gtcgaatatc tgctgatggg
     3241 cgcggcgctg gtgttgttct tcgtgctgct gttagcgttc gccgaggtga tcggctttgc
     3301 ggccgcctat gtgctcgcct cggcggcgat cgccggcctc aacaccgctt actccgccgc
     3361 gatcctcagg agctggcggc gggcggggct gatcggcggc ctgctcgtcg cgctctacgc
     3421 cgtgctctac atcctgctga acctcgaggc ctactcgctg ctgatcggct cgctgctgct
     3481 gttcgcggcg ctggccgggg tgatgtacgg cacccggcgc atcgactgga gcgcccgaag
     3541 cggggaaaag cagccggcct gaggggtttg cggccggcct gttggggaat ggccgcgcct
     3601 cggctagacg ctgggcaatg agtgaatcca tcccgcccga gcgcattttc ccgacctcca
     3661 aggtcgatgc gcaagccgcc aggcatgtgc agtccagccc gcagacggaa agcgaggcct
     3721 acaagctcgc tttccaggac accgacttcc tgctccgccc cgacctgcgc ccggtgcgct
     3781 tccagctcga gctcctgaag cccgagctgc tgctcgacga agcgaagatc cgctcgatga
     3841 tcgtgttcta cggctcggcc cggatcccgg cgccggacat ggccgatgca ctggtcgctt
     3901 ccgccacgac ccccgagcag cgcgcggtcg ccgagcggct caaggccaag tcgcattatt
     3961 acaccgtcgc ctatgagctt gcccggctcg ccagccgcac tccgccggat gccgacggct
     4021 ggcgcgactt cgtcgtctgc tcgggcggcg ggccggcgat catggaagcg gccaaccgcg
     4081 gctcggtcga cgaggggcgc gagaccatcg gcctcaacat cgtcctcccc cacgaacaat
     4141 tgcccaatcg ctacgtgacc ccggaactga gcttccagtt ccactatttc gcattgcgca
     4201 agatgcactt ccttctgcgc gcgcgggcga ttgcggtgtt ccccggcggc tacggcacgt
     4261 tcgacgagat gttcgagctc ctgaccctga tccagaccgg caaggtgcgg ccgctgccga
     4321 tcctgctgtt cggccgcgag ttctggacca aggtggtcga tttccaggcg ctggtcgacg
     4381 agggcgtgat ctcgccccac gacctcgacc tcattcactg gagcgaggac gccgcggaag
     4441 cgtgggacta tgtccagcac ttctacgagg aagaatagcg ggcgcttgcc ctaattcttg
     4501 tcgactcccg acgggccggt caccttcggg gcaccggcgc cgccgacgtt cttttccccg
     4561 cccttggcgg cctgctgttc gttgagcacc ggctcgttct cggccttctg ggcgccgtct
     4621 ccggcgtcag cggccgcttc cgcgggcgcc gcggggaccg gcagcggcgc attgtcgcgc
     4681 gaattgagga aggccatcac gtcggcgcgg tcctgcgggt tgccgaggcc ggcgaaggtc
     4741 atcttggtgc ccggggcgaa cttcttgggg ttggcgagcc aggcgctcat cgcctcccag
     4801 tcccagctgc cgcccttcga cttcagggcg tcggagaaag cgaagcccgg atggccggcg
     4861 acgggattgc ccatcactcc gtacagcgcc gggccgagcg cgttgggccc gccgggctcg
     4921 gcgttgtggc aggcggcgca tttcttgaac acctgctcgc ccttcgccgc gtcggcgctg
     4981 gcgaggtaga aggcgatcgg cttctcggct tcgccttcgg cgccttcgag ctcgacgccc
     5041 tcgatgggat agcccatcgt ctcgggccgc tcggcgtgga acacctcgcc ggtgacgatc
     5101 gacgagccga gcgcgatgat gcccgcgaac agcacccagc cagagacggt ggtgaaacgg
     5161 tcgttcattc agatctccgc tcggccgagc cgacgaatgt cgtggagtgg cccgcccttt
     5221 aggcaggagt ccgggcttct tcaagcgggc gccggtcagg cggcgcggcg gtccccggag
     5281 cgctcgagaa ccagctcggc ggcgcgccgg taaagcgtcg gcaggccggc ctcgccgagc
     5341 cgctcgagcg gctgccacca gccgtccccg agcggcttgc cgccgcgtag gaccgccagc
     5401 tcgagcgcga agtggctgaa aaggtggcgg acccgggcga gcggcggcga cggcaggggc
     5461 ggcggcgaat ccacccactc gccgccgggc agcgccgcca tcccgccgag catccccctc
     5521 ggcggccggc ggaccagcca caccgcgccg tcctgctcga tccaccacgc gaccccgtgg
     5581 cgggtcggcc gggcgcgcct cgccggcgct tgcggaaagg cttcgggcgt gccgctggcg
     5641 cgcgcggcgc agccgctgcg cagcgggcag gtcgggcatt cgggcgcctt ggggcggcag
     5701 acggtcgcgc ccaggtccat catcgcctgg gcgaagtccc cggcgcggcc ggccggggtc
     5761 atggcctcga ccagcacccg gatctcgcgc cgcgaaggct gctccagccc gttcagccgg
     5821 gcgaccaccc gggcgacatt ggtgtcgacc accgcggcgt tgcggccgaa ggcgatcgcg
     5881 gcgatcgccg cggcgctata gtcgccaatc cccgggagcg cccggagccc ggcctcgctc
     5941 gacgggaagc cgccgcgcgc ggccacctcg cgcgcgcagg cgatcaggtt
//
LOCUS       DASTZO010000249         7079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_491684,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000249 DASTZO010000000
VERSION     DASTZO010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7079
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491684"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1155)
                     /locus_tag="VFU20_06975"
     CDS             complement(<1..1155)
                     /locus_tag="VFU20_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286235.1"
                     /translation="MVDRPTDNEGLLSNERSQVVRVRRYRGRRAFMVVGIGLLILLLL
                     ALAGLWVARRPIATGFLEREFERRGVEASYRLDRVGLRTQQVSNLVIGDPRRPDLTAR
                     FAQIQTRIQWNGSVEVYRVVARGVRLRGRLVKGRVSWGQIDKLLPPPTDKPFQLPDFV
                     LDVADSSISLATPFGPLGIALHGAGNLAGGFKGRMAASSPQLIPGQCQIHGLSANLAV
                     AVEARRPHVDGPLRVAHLICPKSRFEIFEPRFDINSRFNEAFTRYDGRGRMAIPRLTA
                     GDNGLAAFIGDLTFRGDPSATYGSVRLAAQRSRLATIFAERTRLAGRYRLGMTGGTFV
                     MVGDYAANGARLDPSMLAAVTGALDAARGTPVGPVATAIGTAVRRTASEFD"
     gene            1295..1987
                     /gene="radC"
                     /locus_tag="VFU20_06980"
     CDS             1295..1987
                     /gene="radC"
                     /locus_tag="VFU20_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU5286236.1"
                     /translation="MRSGATTIADEGSGHRARLRQRLFDGGPKALLDHELIEYLLALA
                     IPRRDTKAQAKKLIAEFGGVGPLLSADSETLARAGLSEGTVAALKIAEATALRLLETR
                     IEGRPLLSNWEALGDYLQAAMAHTAVEEVRVLFLNAKNLLIANEAMWQGSVDEASVHV
                     REVISRAIALGATAIIIVHNHPSGDSTPSTQDIRLTRNLVEAGRHMKIVVHDHVIVGS
                     QGRTSLKALGLI"
     gene            2125..2397
                     /locus_tag="VFU20_06985"
     CDS             2125..2397
                     /locus_tag="VFU20_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HEU5286237.1"
                     /translation="MPQYVIEREMPGAGQLSPEDLKMASQTSCSVLGDLGPGIQWVHS
                     YVTDNKIYCIYRAPNEELIREHAQRGGFPANKISQVHTMIDPTTAE"
     gene            2444..4219
                     /locus_tag="VFU20_06990"
     CDS             2444..4219
                     /locus_tag="VFU20_06990"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEU5286238.1"
                     /translation="MADVFVSYARPDEPHAIRVVEALRASGYGVWRDDQIPAHGCYAD
                     VIEERLKAAKAVVVLWSAEAAKSQWVRAEADAARNAGTLVQASVDGTLPPMPFNQIQC
                     AELRDWASEDEPGWRKLAESVAILAGIRSGPEGAEARTDKRARKRSICVLPFANMSGD
                     AEQEYFSDGISEDITTDLSQVSALSVVARNTAFMFKGQAVDVCEVARKLGVSHVLEGS
                     VRKAASRVRITAQLIDGATGDHVWAERFDRDLDDIFAIQDEMSKAIVAALKLKLLPEE
                     KKAIEQRGTTSAEAYQLYLMARNYWVNGNYGDIRREQRVMRICSRAVEIDPYYAQAWA
                     LLAIAQSSLRYGFGSEVDDGVAAAHSALAIDPSIAEAHCPMARRLEEKGRYEEAVGHI
                     ETALRLDPDSWEVNKEAARAHICAGRVAEATRHFERAVAVMESDVHAWGMLVTCYQAL
                     GNSDGMKRAAEMSVAEAEKVLAHDPSNGAAMGVGAAGFAALGQPESAREWMDRAMLID
                     PDNLNMRYNFACMLAAHLGDQEGALRQLERILAVASGTVVRAAQADPGLASIRDDPRF
                     EKALAAARDRLQIERTGHCRLTGVT"
     gene            4258..5583
                     /gene="purB"
                     /locus_tag="VFU20_06995"
     CDS             4258..5583
                     /gene="purB"
                     /locus_tag="VFU20_06995"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU5286239.1"
                     /translation="MVPRYSRPQMTAIWSPQNRYRIWFAIEVFAAEAMGRAGTIPAED
                     AAKIRAAYDSDALGEIDIAAIDAIETVTKHDVIAFLTWAGSRLGPEARYLHQGMTSSD
                     VLDTALAVQLKQASDLLIADLDELLAVLKRRALEHRLTPTIGRSHGIHAEPTSFGLKL
                     AQAYAEFDRARSRLVAARDEVATCAISGAVGTFANVSPDVEAHVAAELGLTPEPISTQ
                     VIPRDRHAMFFATLGVIASSIERLATEIRHLQRTEVLEAEEFFSPGQKGSSAMPHKRN
                     PVLSENLTGLARMVRSAVTPALENVALWHERDISHSSVERFIGPDATITLDFALARLT
                     GLIDKLLVYPERMRRNLERMGGLVHSQRVLLALTQAGLSREEAYALVQRHAMKVWESD
                     GQSSLLELLGQDPEVTARIAPGELEQLFDLDHHLREVDRIFDRVFGAGA"
     gene            5655..6542
                     /locus_tag="VFU20_07000"
     CDS             5655..6542
                     /locus_tag="VFU20_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5286240.1"
                     /translation="MPAQDADIVQDARAPVAAAADSTGPQIQPAGFLIQLSLDWIVLR
                     ASENVDDFLGQSHVTLIDEPLGRFVQAQALHDLRNLFSRLSGSTGVARAYRVRLAHDR
                     PRFDIAFQVSDGRVLLEGAPSPERGLGEAMGSVAGLVDGLSAHRGEALLEAAARRVRA
                     LTGCDRALILCRDDAGERSAHSSRGRFGDSSIVPDLPAIVADAAAAPVPLFPRKAGGA
                     SIAGALLRSPSAGTLQRLGEQEIASAISVPLSVEGRELGIIQCHSRSARAPRLELHAA
                     AELFAQSFALRLEIDRLRG"
     gene            complement(6539..>7079)
                     /locus_tag="VFU20_07005"
     CDS             complement(6539..>7079)
                     /locus_tag="VFU20_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEU5286241.1"
                     /translation="EVIADPCANCGGEGRALKRRSVSVKIPAGVDEGTRIRVSGEGEA
                     GVRNAAGGDLYIFVHMKRHPIYGREGTTLIAECPVSFTTAALGGSISLPGVDGSRIDI
                     KIPAGIQSGEQLRQRGAGMSVLNGRGRGDLVAKVLVETPRRMSARQKKLLEEFRKTET
                     GDECPAAKSFFQRVRETFG"
BASE COUNT         1113 a         2450 c         2437 g         1079 t
ORIGIN      
        1 gtcgaattcg cttgccgtgc ggcgaaccgc ggtgccgatc gccgtggcga ccgggcccac
       61 gggcgtgccg cgggcggcat ccagcgctcc ggtgaccgcc gccagcatcg acgggtcgag
      121 cctcgcgccg ttcgccgcat aatcgccgac catgacgaac gtgccgccgg tcatcccgag
      181 ccggtagcgg ccggcgagcc gcgtccgctc ggcgaaaatg gtcgccagcc gcgagcgctg
      241 cgcggccagc cggaccgagc cgtaggtcgc cgaagggtca ccgcggaacg tcaggtcgcc
      301 gatgaacgcg gccagcccgt tgtcgccggc cgtcagcctc gggatcgcca tacggccgcg
      361 gccgtcgtag cgtgtgaacg cctcgttgaa gcggctgttg atgtcgaacc gcggctcgaa
      421 gatctcgaaa cggcttttgg ggcagatgag gtgggcgacc cggagcggcc cgtcgacatg
      481 cggccggcgg gcttccaccg ccaccgccaa attggcgctc aggccgtgga tttggcattg
      541 gcccgggatc agttgcgggc tcgacgcggc catccggccc ttgaagccgc cggcaagatt
      601 gccggcgccg tgcagcgcga tcccgagcgg tccgaacggg gtcgcgaggc tgatgctgct
      661 gtcggcgacg tcgagcacga aatcgggcag ctggaacggc ttgtcggtcg gcggcggcag
      721 caatttgtcg atctggcccc agctcacccg ccccttgacc agccggccgc ggagccggac
      781 tccgcgagcg acgacccgat aaacctcgac gctgccgttc cactggatcc gggtctggat
      841 ctgcgcgaag cgggcggtga ggtcgggccg ccgcgggtcg ccgatcacca ggttgctgac
      901 ctgctgggtg cgcagtccga cccggtcgag gcggtagctg gcctcgaccc cgcggcgctc
      961 gaactcgcgc tcgaggaagc cggtggcgat cggccggcgg gcgacccaca gcccggccag
     1021 cgcgagcagc agcaggatca gcaggccgat gccgacgacc atgaaggcgc ggcggccgcg
     1081 atagcgtcgc acgcgaacca cctgggagcg ctcgttcgag agcagcccct cgttgtcggt
     1141 cggcctgtcc accattggct tttactaacc cttgcgaagc tgccccgttc cgtctccggc
     1201 acgaccgccg ggccggctcc ggcgcctgct cgctttccag gagcggcgat ctgtggttga
     1261 ttgccgcttg ctcggcagtc gcgcaggagg cggcatgcgg agcggagcca cgaccatcgc
     1321 cgacgagggc agcggccacc gggcgcgtct tcggcagcgg ctgttcgacg gcggccccaa
     1381 ggcgctgctc gaccacgagc tcatcgaata tctgctggcc ctcgccatcc cgcgccgcga
     1441 caccaaggcg caggccaaga aactgatcgc cgaattcggc ggcgtcgggc cgctgctttc
     1501 ggccgattcc gagaccctgg cccgggccgg gctgagcgaa gggaccgtcg cggccctgaa
     1561 gatcgccgag gcgaccgccc tgcggcttct ggaaacgcgc atcgagggcc ggccgctgct
     1621 gtccaactgg gaggcgctcg gcgactatct gcaggcagcg atggcccata cggcggtcga
     1681 ggaggtgcgc gtgctgttcc tcaacgccaa gaatttgctg atcgccaatg aggcgatgtg
     1741 gcagggctcg gtcgacgagg cctcggtcca tgtccgcgag gtgatcagcc gggcgatcgc
     1801 cttgggcgcc accgcgatca tcatcgtcca caaccacccg agcggcgatt cgactccgag
     1861 cacccaggac atccgcctga cccgcaacct ggtcgaggcc ggcaggcaca tgaagatcgt
     1921 cgtccacgac catgtgatcg tcggctcgca gggccgcacc agcctgaagg cgctggggct
     1981 gatctgaacg gtgcgctatc gacttcgatg tcgcctcgcc ggcgataggg aagcacggtc
     2041 ggccgcgacg ccgctgcgga catccgtcgg tgccggtgct attgcggccg tccttggtgt
     2101 aacgctggat aaggagcagc ctagatgcct caatatgtga tcgaacggga gatgccgggc
     2161 gccgggcaat tgagccccga agacctgaag atggcgtcgc agacgtcctg ctcggtgctt
     2221 ggcgacctcg ggcccggcat ccagtgggtc cacagctacg tcaccgacaa caagatctac
     2281 tgcatctatc gcgcccccaa cgaggagctg atccgcgagc atgcgcagcg cggcggcttc
     2341 ccggccaaca agatttcgca ggtccacacg atgatcgacc cgacgacggc cgagtaagcg
     2401 ccggcgagac tcgccgccgg agtcctgata tcccgcaagg cttatggcgg acgtgttcgt
     2461 ctcctatgcg cggccggacg agccgcacgc gatccgcgtc gtcgaggcgc tgcgggccag
     2521 cggctatgga gtctggcgcg acgaccagat tccggcccac ggatgctatg ccgacgtcat
     2581 cgaggagcgg ctcaaggcgg ccaaggcggt ggtcgtgctg tggtcggccg aagccgcgaa
     2641 atcgcagtgg gtgcgcgccg aggcggacgc cgcgcggaat gccgggaccc tggtccaggc
     2701 gagcgtcgat gggaccctgc cgccgatgcc cttcaaccag atccagtgcg ccgagctgcg
     2761 cgactgggcg agcgaggacg agcccggctg gcgcaagctc gccgaaagcg tcgccatcct
     2821 cgccggcatc cgcagcggtc ccgaaggagc cgaagcgcgg acggacaagc gcgcgcgcaa
     2881 gcgctcgatc tgcgtgctcc ccttcgccaa catgagcggc gatgccgagc aggaatattt
     2941 ttccgacggc atcagcgaag acatcaccac cgacctgtcc caggtctcgg ccctctcggt
     3001 cgtcgcgcgc aacaccgcct tcatgttcaa ggggcaggcg gtggatgtct gcgaagtcgc
     3061 ccgcaagctc ggggtcagcc acgtcctcga aggcagcgtc cgcaaggcgg caagccgggt
     3121 tcggatcacc gcccagctga tcgacggggc gaccggcgac catgtctggg ccgagcggtt
     3181 cgaccgcgac ctcgacgaca ttttcgccat ccaggacgag atgtcgaagg cgatcgtcgc
     3241 ggcgctcaaa ttgaagctgc tccccgagga aaagaaggcg atcgagcagc gcggcacgac
     3301 cagcgccgag gcctatcaac tctatttgat ggcccgcaac tactgggtga acggcaatta
     3361 tggcgacatc cgccgcgagc agcgcgtgat gcgcatctgc agccgcgcgg tcgagatcga
     3421 cccttattac gcccaagcct gggcgctgct ggccatcgcc cagtccagcc tgcgctacgg
     3481 cttcgggagc gaggtcgacg acggggtcgc ggccgcgcat agcgcgctcg ccatcgaccc
     3541 gagcatcgcc gaagcgcatt gcccgatggc ccggcggctc gaggagaagg gacgttacga
     3601 agaggcggtc ggccacatcg aaaccgcgct tcggctcgac cccgattcgt gggaagtgaa
     3661 caaggaagcc gcgcgggccc atatctgcgc cggccgggtg gccgaggcga ccaggcattt
     3721 cgagcgggcg gtcgcggtca tggaatccga cgtccatgcc tggggaatgc tggtcacctg
     3781 ctaccaggcg ctgggcaatt ccgacgggat gaaacgggcg gcggaaatgt cggtcgccga
     3841 ggcggaaaag gtgcttgcgc acgaccccag caacggggcc gcgatggggg taggcgccgc
     3901 cggcttcgcg gctttgggtc agccggaatc ggccagggaa tggatggacc gggcgatgct
     3961 gatcgacccc gacaatctca acatgcgcta caatttcgcc tgcatgctgg ccgcgcatct
     4021 cggcgaccag gagggcgccc tgaggcagct cgagcgcatt ctcgcggtgg cgagcggcac
     4081 cgtggtccgc gccgcgcagg ccgatcccgg cctggcctcg atccgcgacg acccgcgctt
     4141 cgaaaaggcg ctcgccgcgg cccgggaccg gctccagatc gagcgcaccg gccactgccg
     4201 cctgacgggt gtcacatgat ttgatgcggc gcggcggctc tgctagccgc gccggggatg
     4261 gtcccccgct actcccgccc gcagatgacc gccatctggt cgccgcaaaa ccgctatcga
     4321 atctggttcg cgattgaggt gttcgcggcc gaagcgatgg gccgagccgg cacgatcccg
     4381 gccgaggacg cggcgaaaat ccgcgccgcc tacgactccg acgcgctcgg cgagatcgac
     4441 atcgcggcga tcgacgcaat cgaaacggtc accaagcatg acgtgatcgc tttcctgacc
     4501 tgggcgggaa gcagactcgg cccagaggcg cgctacctcc accaggggat gaccagctcc
     4561 gatgtgctcg acaccgcgct cgcggtgcag ttgaagcagg cgtcggacct gctgatcgcc
     4621 gatctcgatg aattgctcgc agtgctgaag cggcgcgcgc ttgagcacag actgaccccg
     4681 accatcggcc ggagccacgg aattcacgcc gagccgacca gcttcggcct caagctcgcc
     4741 caggcctatg ccgagttcga ccgcgcccgc tcgcgcctgg tcgcggcgcg cgacgaggtc
     4801 gcgacctgcg cgatctcggg cgcggtcggc actttcgcca atgtctcgcc cgacgtcgag
     4861 gcgcatgtcg ccgccgagct cggcctcacg cccgagccga tctcgaccca ggtgatcccg
     4921 cgcgaccgcc acgcgatgtt cttcgccacg ctgggggtga tcgcctcgtc gatcgagcgg
     4981 ctggcgaccg agatccgcca tttgcagcgg accgaggtgc tcgaggccga ggagtttttc
     5041 tctcccggcc agaaaggcag ctcggcgatg ccgcacaagc gcaacccggt gctgagcgag
     5101 aatctgaccg gcctcgcgcg gatggtgcgc agcgccgtca cgcccgcgct cgagaatgtc
     5161 gctctgtggc acgagcgcga catttcgcac tcgtcggtcg agcgcttcat cggccccgac
     5221 gcgacgatca cgctcgattt cgcgctggcg cggctgaccg gcctcatcga caaattgctc
     5281 gtctatcccg agcggatgcg caggaacctc gagcgcatgg gcgggctcgt ccattcgcag
     5341 cgggtgttgc tggcgctgac ccaggccggg ctcagccgcg aggaggctta tgcgctggtc
     5401 cagcgccacg cgatgaaggt gtgggaatcc gacggccaat cgtcgcttct cgagctgctc
     5461 gggcaggacc ccgaggtcac cgcgcgaatc gctccgggcg agctcgagca attgttcgac
     5521 ctcgaccatc acctgcgcga ggtcgaccgg attttcgatc gggtgttcgg cgccggcgcc
     5581 taaccgctcg gtttcagaac tttttctggg ttgcgcggcc cgatcctgcg ctatgatgcg
     5641 cgcgggggcc agggatgcca gctcaagacg ccgacatcgt gcaggacgcg cgcgcgccgg
     5701 tcgcggccgc tgcggactcg accgggccgc agatccagcc ggccggcttc ctaatccagc
     5761 tgtcgctcga ctggatcgtc ctgcgcgcgt ccgagaacgt cgacgatttc cttgggcaat
     5821 cgcacgtcac cctgattgac gagccgctcg gccgcttcgt ccaggcgcag gcgctgcacg
     5881 accttcgcaa cctcttttcc aggctcagcg gttcgaccgg agtcgcgcgg gcctaccggg
     5941 tgcggctcgc ccacgaccgg ccgcgcttcg acatcgcctt ccaggtcagc gacggccggg
     6001 tgctgctcga gggggcgccc agccccgaac gggggctcgg agaagcgatg ggatcggtcg
     6061 cgggcctggt cgacggcctt tccgcgcacc gcggcgaggc gctgctcgag gcggccgcgc
     6121 ggcgcgtccg ggcgctgacc ggctgcgacc gggcgctgat cctgtgccgc gatgacgccg
     6181 gcgagcgcag cgcgcacagc agccgcggcc gcttcgggga ttccagcatc gtccccgacc
     6241 tgccggccat cgtcgccgac gcggccgccg ctccggtgcc gctgttcccg cgcaaagccg
     6301 gcggcgcctc gatcgccggg gcgctgttga ggtcgccgtc agccggcacc ctgcagcggc
     6361 ttggcgagca ggagatcgcc tcggcgatca gcgtcccgct gagcgtcgag gggcgcgagc
     6421 tggggatcat ccagtgccac agccgttcgg cgcgagcgcc gaggctcgag ctgcacgccg
     6481 cggccgagct gttcgcgcaa tcgttcgccc tgcgcctcga aatcgaccgc ctgcgcggct
     6541 agccgaacgt ctcgcgcacc cgctggaaga agcttttcgc cgccgggcat tcgtccccgg
     6601 tctcggtctt gcggaattcc tcgagcagct tcttctgcct ggcgctcatc cgccgcggag
     6661 tctcgaccag cactttggct accagatcgc cgcggccgcg gccgttgaga acgctcattc
     6721 ccgcgccgcg ctgccgcagc tgctcgccgg actggatccc ggccgggatc ttgatgtcga
     6781 tcctgcttcc gtcgacccct ggcaggctga tcgagccgcc gagcgcggcg gtggtgaagc
     6841 tgaccgggca ttcggcgatc agggtcgtcc cttcccggcc gtagatcggg tgccgcttca
     6901 tgtggacgaa gatgtagagg tcgccgccgg ccgcgttgcg aacgccggcc tcgccctcgc
     6961 ccgagacccg gatgcgggtg ccttcgtcca ccccggcggg gatcttgacc gacacgctgc
     7021 ggcgcttgag cgcccggcct tcgccgccgc aattggcgca cgggtcggcg atcacctcg
//
LOCUS       DASTZO010000250         6750 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_491713,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000250 DASTZO010000000
VERSION     DASTZO010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491713"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /locus_tag="VFU20_07010"
     CDS             <1..387
                     /locus_tag="VFU20_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HEU5286242.1"
                     /translation="DDVFGSFTSREVAADKQLWLEAGEKMLFAGGTKGLALDTERLVL
                     KVVEGDDPSVLVHDPRNRGVAAMLIEMPESFPMALGVIYEDPAPTFESAVIEQNRAAA
                     TGKSPDLQQLVSKGQTWMVEKEPHKL"
     gene            407..1336
                     /locus_tag="VFU20_07015"
     CDS             407..1336
                     /locus_tag="VFU20_07015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU5286243.1"
                     /translation="MDNLTHSLAGALLGQLGLKRLTGRAMATLVIAANLPDIDALYRF
                     AGPGIGFRRGITHGPVALLVLPLLLAALVLAFDRLWPKRGGPQVRPWAVLLLAFIGTL
                     SHPLLDWLNSYGIRFLEPFSSRWFYGDTLFIIDVWLWVALLASVHLSQMRERKAHAGW
                     RRPALVGLGAIFAYVAANVLLSTVAERRTEERLIVERKVQPTMVVANPVPLTPWRRHM
                     LWRDEARHGSGSYALGAGVRLDPGAIANGVDNPALARAAARDEEVRAYLFWSRMPIVV
                     REGGRTFLGDQRFVGRRTRGYFLIPLDSAGPAP"
     gene            1347..2594
                     /locus_tag="VFU20_07020"
     CDS             1347..2594
                     /locus_tag="VFU20_07020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEU5286244.1"
                     /translation="MAGRGEHLITADRGFAVGGGAVGRIVSPAFARVLDQLDRRLVSG
                     GIEATLPDGSQRRIGCRAAGPVAVVHLNSWMALVRLATSGSVGWYKAWALGEWTSPDP
                     VALFELFTANAVELGDSARAKGPFRWVNALAHRLRDNAPGKARANVAAHYDLGNDFYA
                     AWLDETMTYSCARFDSPDQPLEAAQLRKIALLLDRLDLRPGQRLLEIGCGWGSLAIEA
                     ARRGVAVVGLTLSEQQKAWADAKVAEAGLADRVEIRLQDYREIDERFDAVASVEMVEA
                     VGRRWWGAYLDSVARNLGPGGRAALQFISIDHRLFERYAASADFIQTYIFPGGMLLDE
                     PHFEMLARERQLSWDGREGFAVDYAETLTRWRARYDDAVARGDLDGFGEQFHRLWRYY
                     LMYCEGGFRGGAIDVAQVTMVKA"
     gene            complement(2591..3307)
                     /locus_tag="VFU20_07025"
     CDS             complement(2591..3307)
                     /locus_tag="VFU20_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5286245.1"
                     /translation="MTDEKPLAGKLALVTGASRGIGAATAEALGAAGAHVVLVARTAS
                     ALEAVDDRIHAAGGSATIAPLDLTDSESIGKLAAAVAERWDRLDILILNAAMLGSLTP
                     VEHIDPKEYGRILGLNLIANQAMIAAFDPLLRKAERADVVALTSSVGSEPRAFWGAYG
                     SSKAALETLLGAYADETEHSGRVRVHIVDPGASRTRMRQLAFPGEEPDSVKPPEVAAA
                     AILERLLSEVPSGEKVRVET"
     gene            complement(3320..4783)
                     /gene="purF"
                     /locus_tag="VFU20_07030"
     CDS             complement(3320..4783)
                     /gene="purF"
                     /locus_tag="VFU20_07030"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU5286246.1"
                     /translation="MFSTNPFDPFGPDGDKLREECGIFGIWGADNAASFVALGLHALQ
                     HRGQEAAGITSFDGTHFHSHRAMGHVAGNFDRDEVMRALGGRTAIGHVRYSTTGETAL
                     RNVQPLFAELAEGGLAVAHNGNLSNAMKLRRTLNRRGSIFQSTSDTEVIIHLVATSQY
                     ATTVERLIDALRQVEGAYSLLVLTEEGLIACRDPLGIRPLVMGKLGEATIFASESVAL
                     DVIGATFLREVEPGEIVLVNGSGIRSFRPFEKADPKPCIFEHVYFSRPDSIVDGTSVY
                     QVRKNIGAELAREAPVEADYVVPVPDSGVPAAIGFSQESGIPFELGIIRSHYVGRTFI
                     EPSDGIRHLGVKLKHNANSGLVAGKRIVLIDDSIVRGTTSLKIVQMMRDAGAREVHMR
                     IASPPTRHSCFYGVDTPERAKLLAAQMGVEAMADYINADSLAFISIEGLYRALGSERN
                     NAQPQRCDACFTGEYPTSLTDFSEKERAGSLSLVASR"
     gene            4952..5341
                     /locus_tag="VFU20_07035"
     CDS             4952..5341
                     /locus_tag="VFU20_07035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEU5286247.1"
                     /translation="MESGTRYAIGELCEAFGVTPRALRFYEDEQLIAPERRGTARLYS
                     DRDRARLSWILRGKRVGFSLSEIREMLDLYDLGDHRQTQRRVTIERCRERIAALRRQK
                     DDIDATIAELEAFLTTLGSPNNQGSTE"
     gene            5341..>6750
                     /locus_tag="VFU20_07040"
     CDS             5341..>6750
                     /locus_tag="VFU20_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5286248.1"
                     /translation="MAVYAAPVRETRFILDQVLEVGRYSNLQGFANATPDLIEAILDE
                     GGKFAAEVLAPLNKVGDEQGCKRRDDGSVTVPDGFKDAYRLWCEGGWPTLSAPEEYGG
                     QGLPQVLSTALSEYVLSANQSFEMFQGLTSGAIASLVIKGSDEQKAKYLPNMVSGRWT
                     GTMNLTEPHCGTDLGLLKTRAEPNPDGSYSITGTKIFISSGEHDLSENIIHLVLAKIA
                     GAPDTVKGISLFVVPKFLVNDDGSIGERNPVSCGAIEKKMGIHGNPTCVLNYDGAKGW
                     LVGEPEKGLAAMFIMMNAARLGVGLQGLAQAEVAYQNALAYARERRQGRALVAGEREP
                     DAKADPIIVHPDVRRMLMEMKSFLEGARALVLWGALMVDLSRRAESEEERQEADDLVS
                     LLTPVIKGYLTDKGFESAVLAQQVFGGHGYIREHGMEQFVRDARITQIYEGTNGIQGL
                     DLVGRKLSQGMGRLLRRFFH"
BASE COUNT         1067 a         2201 c         2388 g         1094 t
ORIGIN      
        1 gacgacgtgt tcggctcctt cacctcgcgc gaggtcgcgg ccgacaagca attgtggctc
       61 gaggcgggcg agaagatgct gttcgcgggc ggcaccaagg ggctcgcgct cgacaccgag
      121 cggctggtgc tcaaggtcgt cgagggcgac gacccgtcgg tgctggtcca tgacccgagg
      181 aaccgcgggg tcgcggcgat gctgatcgag atgcccgaga gcttcccgat ggcgctcggc
      241 gttatctacg aggacccggc gccgacgttc gaaagcgccg tcatcgagca gaaccgcgcc
      301 gcggcgaccg gcaaatcgcc cgacctccag cagctggttt ccaagggcca gacgtggatg
      361 gtcgagaagg aaccgcacaa gctctgaagc cggcccgcgt ccccgcatgg ataatctgac
      421 ccacagcctc gctggggccc tgctgggaca actcgggctg aagcggctga ccggccgcgc
      481 gatggcgacc ctggtgatcg ccgccaacct gcccgacatc gacgctctct accgcttcgc
      541 ggggccggga atcggttttc gccgcggaat cacccacggg ccggtggcgc tgctcgtcct
      601 gccgctgctg ctggctgctc ttgtgctcgc attcgatcgc ctctggccga agcgcggcgg
      661 gcctcaggtg cgcccctggg cggtgctcct gctcgccttc atcggcaccc tcagccatcc
      721 tctgctcgat tggctcaaca gctacgggat ccgctttctc gaacccttct cgagccgctg
      781 gttttacggc gacaccttgt tcatcatcga cgtctggctg tgggtcgcgc tgctcgcatc
      841 ggtccacttg tcgcagatgc gggagcgcaa ggcgcacgcc ggctggaggc gacccgcgct
      901 ggtcgggctc ggcgccattt tcgcttatgt cgcggcgaac gtcctgttga gcaccgtcgc
      961 cgagcggagg accgaggagc ggctgatcgt cgagcgaaag gtgcagccga caatggtcgt
     1021 tgccaatccg gtcccgctga cgccgtggcg gcggcacatg ctgtggcggg acgaggcccg
     1081 gcacggaagc ggaagttacg cgctcggcgc tggagtgcga cttgacccgg gcgccatcgc
     1141 caatggcgtc gacaacccgg ccctcgcgcg tgcggcggcg cgcgacgagg aggtacgcgc
     1201 atacctgttc tggtcgcgga tgccgattgt cgttcgtgaa ggcggtcgca ccttcctcgg
     1261 cgaccagcgc ttcgtcgggc gccgaacccg tggctacttc ctgatcccgc tcgacagcgc
     1321 cggcccggct ccatagcctt gaacccatgg cgggtcgcgg cgagcatctg atcacggcgg
     1381 atcgcggctt cgccgtcggc ggcggcgcgg tcgggcggat cgtctcgccg gcctttgcca
     1441 gggtgctgga ccagctcgat cgccggctgg tgagcggcgg gatcgaggcg accctgccgg
     1501 acggaagcca aaggcggatc ggctgtcgcg ccgccgggcc ggtcgccgtc gtccatctca
     1561 acagctggat ggcgctcgtg cggctggcga cgtcgggctc ggtcggctgg tacaaggcgt
     1621 gggcgctcgg cgaatggacg agccccgacc cggtcgcgct gttcgagctg ttcaccgcca
     1681 acgccgtcga gctgggcgat tcggcgcggg ccaaggggcc gttccgctgg gtgaacgcgc
     1741 tcgcccaccg gctgcgcgac aatgcgccgg gcaaggcgag ggccaacgtc gccgcgcatt
     1801 acgacctcgg caacgatttc tacgccgcct ggctcgacga gacgatgacc tattcctgcg
     1861 ctcggttcga ctcgccggac cagccgctcg aggccgcgca attgcgcaag atcgcgctgc
     1921 tgctcgaccg gctggatctc cggccgggcc agcggctgct cgaaatcggt tgcggctggg
     1981 gcagcctggc gatcgaggcg gccaggcgcg gcgtcgcggt ggtcgggctg accctgtcgg
     2041 agcagcagaa agcctgggcc gacgcgaagg tcgccgaggc gggcctcgcc gatcgggtcg
     2101 agatccggct gcaggattac cgcgagatcg acgaacggtt cgacgcggtc gcgtcggtcg
     2161 agatggtcga ggcggtgggc aggcgctggt ggggggccta tctcgattcg gtcgcccgca
     2221 acctggggcc cggcggccga gcggcgctgc agttcatcag catcgatcac cggttgttcg
     2281 agcgctacgc ggccagcgcc gacttcatcc agacctacat ctttcccggc gggatgctgc
     2341 tcgacgagcc gcacttcgag atgctggcgc gggagcggca gctgtcgtgg gacggccgcg
     2401 aaggatttgc cgtggattat gccgagaccc tgacgcgctg gcgggcgcgg tacgacgatg
     2461 ccgtggcgcg cggcgacctc gacggcttcg gcgagcagtt ccaccggctg tggcgctatt
     2521 atctgatgta ttgcgaaggc ggcttccgcg gcggcgcgat cgacgtcgcg caggtgacga
     2581 tggtcaaggc ctaggtctcg acgcgcacct tctcgccgct cggaacttcg gagagcaagc
     2641 gttcgaggat cgccgcggcc gccacctccg gcggcttgac gctgtccggc tcctcgccgg
     2701 ggaaggcgag ctggcgcatc cgcgtgcggc tcgctcccgg gtcgacgatg tggactcgga
     2761 cccggccgct gtgctcggtc tcgtcggcat aggcgccgag cagggtctcg agcgcggcct
     2821 tggacgagcc gtaggcgccc cagaaggcgc gcggctcgct tccgaccgag gacgtcaaag
     2881 cgacgacgtc ggcgcgctcc gcctttcgga gcaggggatc gaaggcggcg atcatcgcct
     2941 ggttggcgat gaggttgagg ccgaggatgc ggccatattc cttcgggtcg atgtgctcga
     3001 ccggggtcag cgagccgagc atcgccgcgt tgaggatcag gatgtcgagc ctgtcccagc
     3061 gctcggcgac cgccgcggcc agcttgccga tcgattcgct gtcggtgagg tcgagcgggg
     3121 cgatggtcgc gcttccgccg gctgcgtgga tccggtcgtc gaccgcctcg agcgcgctcg
     3181 ccgtcctggc gaccaggacg acatgggcgc cggcggcgcc aagcgcctcc gcggtcgccg
     3241 cgccgatgcc gcggctggct ccggtgacga gcgcgagctt gccggcgagg ggcttttcat
     3301 ccgtcatggg tcgagtgggc taacggctgg cgaccagcga aagcgaaccg gcccgttcct
     3361 tttcggaaaa atcggtcagg ctggtcggat attcaccggt gaagcaggcg tcgcagcgct
     3421 gcggctgcgc gttgttccgc tccgagccga gggcccggta caggccctcg atcgaaatga
     3481 aggcaaggct gtcggcgttg atgtagtcgg ccatcgcctc gacacccatc tgggcggcga
     3541 gcagcttggc gcgctcgggg gtgtcgaccc cgtagaagca gctgtggcgg gtcggcggcg
     3601 aggcgatccg catgtggact tcgcgggcgc cggcatcgcg catcatctgc acgatcttca
     3661 ggctggtggt gccgcggacg atcgaatcgt cgatgaggac gatgcgcttg ccggccacca
     3721 ggcccgaatt ggcgttgtgc ttcaatttga cgcccaggtg acgaatgccg tcgctgggct
     3781 cgatgaaggt gcggccgaca taatgcgagc ggatgatccc gagctcgaac gggatgcccg
     3841 attcctggct gaagccgatc gcggccggga ccccgctgtc cgggaccggg acgacatagt
     3901 cggcctcgac cggggcttcg cgggcgagct cggcgccgat gttcttgcgc acttggtaga
     3961 ccgaggtgcc gtcgacgatg gaatcggggc gcgagaaata gacgtgctcg aagatgcacg
     4021 gcttgggatc ggccttctcg aacggccgga acgaccggat ccccgagcca ttgacgagga
     4081 cgatctcgcc gggctcgacc tcgcggagga acgtggcgcc gatcacgtcg agcgcgacgc
     4141 tttccgaagc gaaaatggtc gcctcgccga gcttgcccat caccagcggg cggatgccga
     4201 gcggatcgcg gcaggcgatc agcccttcct cggtcaggac gagcagcgag taggcgccct
     4261 cgacctggcg gagcgcgtcg atcagccgct cgaccgtggt cgcgtattgc gaggtggcga
     4321 cgagatggat gatgacctcg gtgtcgctgg tcgactggaa gatcgagccg cggcggttga
     4381 gggtccggcg aagcttcatc gcgttcgaaa ggttgccgtt gtgggcgacc gcgagaccgc
     4441 cttcggcgag ctcagcgaac agcggctgga cgttgcgcag ggcggtctcg ccggtggtcg
     4501 aatagcgcac atggccgatc gcggtccggc cgccgagcgc ccgcatcacc tcgtcgcggt
     4561 cgaagtttcc ggcgacgtgg cccatcgccc ggtgcgagtg gaaatgggtg ccgtcgaagc
     4621 tggtgatccc ggcggcttcc tggccgcggt gctggagcgc gtgaagcccg agcgcgacga
     4681 agctggcggc attgtcggcg ccccagatgc cgaaaatgcc gcattcctcg cgcagcttgt
     4741 ccccgtctgg tccaaaaggg tcgaacggat tggtcgaaaa catgtccccg cctcggcacg
     4801 ctgaacagac cgctcatata gggtcgcttg ccggccttgt cgccctttgc ctgaaccgaa
     4861 attgaaacca aggagcggtt tgccgaccgc ttaagctcgc cacccgtcat tcctgcttga
     4921 cgcttacgga aacggaaacc atagcgcggc gatggagtcg ggtacgagat acgcgatcgg
     4981 cgagctgtgc gaggcgttcg gggtgactcc gcgggcgctt cgattctacg aggacgagca
     5041 gctgatcgct cccgagcggc gcggcaccgc gcggctttac tccgaccgcg accgggcccg
     5101 gctgagctgg atcctgcgcg gcaagcgggt cggcttctcg ctgagcgaaa tccgcgagat
     5161 gctcgacctc tacgacctcg gcgaccaccg ccagacccag cgccgggtga ccatcgagcg
     5221 ctgccgggag cggatcgccg cgctccgtcg ccagaaggac gacatcgacg cgaccatcgc
     5281 cgagctcgag gccttcctga ccaccctggg cagccccaac aaccaaggga gcaccgaata
     5341 atggccgttt atgcagcccc cgtgcgcgaa acgcgcttca tcctcgacca ggtgctcgaa
     5401 gtcggccgct attcgaacct gcagggcttc gccaacgcca cccccgacct catcgaggcg
     5461 atcctcgacg agggcggcaa gttcgccgcc gaagtgctgg cgccgctgaa caaggtcggc
     5521 gacgagcagg gctgcaagcg gcgcgacgac ggctcggtca ccgtccccga cgggttcaag
     5581 gacgcctacc ggttatggtg cgaaggcggc tggccgaccc tgtcggcgcc ggaggaatat
     5641 ggcggccagg gcctgccgca ggtgctgtcc accgcgctca gcgaatatgt gctgtcggcc
     5701 aaccagtcgt tcgagatgtt ccaggggctg acgtccgggg cgatcgcatc gctggtgatc
     5761 aagggcagcg acgagcagaa ggccaaatat ctgcccaaca tggtctccgg ccgctggacc
     5821 gggacgatga acctgaccga gccgcactgc ggcaccgacc tcgggctgtt gaagacccgc
     5881 gccgagccca acccggacgg aagctattcg atcaccggca ccaagatctt catctcgtcg
     5941 ggcgagcacg acctttcgga aaacatcatc cacctggtgc tggcgaaaat cgccggagcg
     6001 cccgacacgg tcaaaggcat ctcgttgttc gtggtgccca agttcctggt caatgacgat
     6061 ggctcgatcg gcgagcgcaa tcccgtcagc tgcggggcga tcgagaagaa gatgggtatc
     6121 cacggcaacc cgacctgcgt gctcaactac gacggcgcca agggctggct ggtcggcgag
     6181 ccggagaagg gactggcggc gatgttcatc atgatgaacg cggcccggct cggggtcggc
     6241 ctgcagggcc tcgcccaggc cgaggtcgcc taccagaatg cgctcgccta cgcccgcgaa
     6301 cggcggcagg gccgggcgct ggtcgccggc gagcgcgagc ccgacgccaa ggccgacccg
     6361 atcatcgtcc acccggacgt ccgccggatg ctgatggaga tgaagtcgtt cctcgagggc
     6421 gcccgggcgc tggtgctgtg gggcgcgctg atggtcgacc tgtcgcgccg cgccgaaagc
     6481 gaggaggagc ggcaggaggc cgacgacctc gtctcgctgc tgacgccggt gatcaagggc
     6541 tatctgaccg acaagggctt cgagtccgcg gtccttgccc agcaggtgtt cggcggccac
     6601 ggctacatcc gcgagcacgg catggagcag ttcgttcgcg acgcccgaat cacccagatc
     6661 tacgaaggca ccaacggcat ccagggcctc gacctggtcg gccgcaagct gtcccagggc
     6721 atgggccgtt tgttgcgacg gttcttccat
//
LOCUS       DASTZO010000251         5305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_382066,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000251 DASTZO010000000
VERSION     DASTZO010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_382066"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2024
                     /locus_tag="VFU20_07045"
     CDS             <1..2024
                     /locus_tag="VFU20_07045"
                     /inference="COORDINATES: protein motif:HMM:NF023167.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YdbH domain-containing protein"
                     /protein_id="HEU5286249.1"
                     /translation="TGPSGARARVSGRDGVTYYWPTGRLRVDGLIEMAGGGLPTGRVD
                     IRQPRSGAPMSGVAEIAPYRAGQSRLELAPIRFAAARDGSTAVSTVLLVDGPFEDGRV
                     RALRVPLTGRFGSRTGVAFGTACAEISWAHVQFRALQIGPTRLPVCPIGPAMVSQAPG
                     GPARWGARLSRPVLAGRLGNSPLRLAASSGRIEGKRFDLDALALRLGRATSPVIFDAK
                     RLQGDFVGRGVAGTFSGASSTIGNVPLAMSDIAGRWRVYKGDLTIDGGMLVADRSPAP
                     RFYPLRSDDVRFSLAGDFVRAAGTLKHPASGTRVTDIAIEHRLSTGAGHALLDVPGIR
                     FGENLQPEELTRLTEGVIALVQGTVIGKGRIDWSSEGKVTSTGEFSTVGMDLAAPFGP
                     VTGLTTTVRFTDLLGLETAPGQSATIASINPGILVENGVIRYQLLRGQLVKVERGEWP
                     FMGGRLILRETILNFGRPSAKRLTFEVVGLDAKTFVDSLGFSGIEATGVFDGVLPMIF
                     DESGGRIVGGRLDSRPGGGSLAYTGVVNKANLGMMGNIAFDALRDLRFRSMVVRLDGE
                     LAGEFTTRLTIDGVALGQTGTQKIIRGLLKKLPLKLNVSIRGPFRALIATAKSFRDPR
                     TVIQPVLPVPLDAPGIVTEVRRHEEQKQQTQTPPDEEIKTTPPATTNER"
     gene            2027..2260
                     /locus_tag="VFU20_07050"
     CDS             2027..2260
                     /locus_tag="VFU20_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006961957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HEU5286250.1"
                     /translation="MAHSRKLRRPALLAAAVFALPLSGCITVEPPDKPIEINLNVAIQ
                     QEVLVRLQRDVEQLIQQNPQAFPQNPPERPRPQ"
     gene            2257..2628
                     /locus_tag="VFU20_07055"
     CDS             2257..2628
                     /locus_tag="VFU20_07055"
                     /inference="COORDINATES: protein motif:HMM:NF018701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HEU5286251.1"
                     /translation="MTRLWPILAAAMAAAAVPAPAQTPAVDAARHAGQVGERFDGYMG
                     FVVAPPATVRSQVSTINIRRRALYSNLAASKGVSPQEVGITAGCQLLARVQVGHAYML
                     VDGQWRRRLAGQPAPVPEYCR"
     gene            2771..3103
                     /locus_tag="VFU20_07060"
     CDS             2771..3103
                     /locus_tag="VFU20_07060"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HEU5286252.1"
                     /translation="MATDETGQDPKLPPDARLDSLEERLERAQAVEAKRTHKAEYNPT
                     ARIWQLMFGHLVGAPAGGGIIGWGLDSLFGTFPTLFLLMLFVGFGVGVVNVMRLSRTP
                     PGEGSEKR"
     gene            3113..3889
                     /locus_tag="VFU20_07065"
     CDS             3113..3889
                     /locus_tag="VFU20_07065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEU5286253.1"
                     /translation="MAAESKIDPMHQFTVEPLLPLNVGGYDVSFTNSSLWLLIALVVI
                     YGFMAMGMRRQLVPNRWQVAVEGLTGFIDDMVKVNIGPEGKKFVPYIFSLFVFILTAN
                     LLGLFPLSIIPGQHQFTTTSHFSITGVLAVLSFSIVLIVGFWRHGLHFFSLFVPHGTP
                     KVMMPLIAAIEFISFMVRPFSLGLRLFVAMIAGHILMEVFGSFIVTGFNGGAMGVGVG
                     LLSFAFIIFVTALELLVCAIQAYVFALLTTLYLNDAINLH"
     gene            3915..4151
                     /locus_tag="VFU20_07070"
     CDS             3915..4151
                     /locus_tag="VFU20_07070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017665361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEU5286254.1"
                     /translation="MDLTSAQVIGAGLAAIGVGAAAIGVGNVFGSFLESALRNPAAAD
                     GQQGRLFIGFAAAELLGLIAFAVAMIILYAPPSA"
     gene            4172..4663
                     /locus_tag="VFU20_07075"
     CDS             4172..4663
                     /locus_tag="VFU20_07075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003044101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEU5286255.1"
                     /translation="MPQINQLSEIFFSQLFWLLVVFGIIYFGIARGMVPKIQSTVELR
                     EKKIADDLAAAQAARAAAEETEAEYRARIDESRAQAAKLTREARQASALETEAKVRAA
                     SEQIARKVAAAEAEIRAAAEAARTEVESVAVEATQEMVALLTGAKVDRGQAAAAVKAE
                     LHG"
     gene            4656..5213
                     /locus_tag="VFU20_07080"
     CDS             4656..5213
                     /locus_tag="VFU20_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286256.1"
                     /translation="MAEPHPEGVATVEHDSGAAHAEPTAFGLTAPMFIGLAMLVVFAI
                     LVWKKVPAAIGKALDAKIALIRDQLAEAEALRKEAEALKKEYQAKAKSADAEAAAVIE
                     RAHGEAGEIVAKAKSDAEGLIERRSRMAEDKIAAEERAAIKQLRAAAADAAARAAARL
                     IAERDDPAADEQLVNRTIAGLGKAN"
BASE COUNT          812 a         1835 c         1833 g          825 t
ORIGIN      
        1 tcacgggtcc cagcggcgcc cgggcgcggg tctccggccg cgacggagtg acttattact
       61 ggccgaccgg ccggctgcgg gtcgacgggc tgatcgagat ggccggtggc ggcttgccca
      121 ccggacgcgt ggatatccgc cagccgcgca gcggcgcgcc gatgagcggg gtcgccgaga
      181 tcgcgcccta ccgggccggc cagtcgcggc tcgagctggc gccgatccgg ttcgccgccg
      241 cgcgcgacgg ctcgaccgcc gtcagcacgg tgctgctggt cgacggtcca ttcgaggacg
      301 ggcgggtgcg ggcgttgcgc gtcccgctga ccgggcgctt cggaagccgc accggagtcg
      361 ccttcgggac ggcctgcgca gagatcagct gggcccacgt gcaatttcgc gcgctccaaa
      421 tcggccccac gcgtcttccg gtgtgcccga tcggcccggc catggtctcg caggcgcccg
      481 gcggcccggc tcgctggggc gcccgcctgt cgcgcccggt gctcgccggc cggctcggca
      541 attcgccgct gcggctcgcg gcctcgagcg gacgaatcga gggcaagagg ttcgacctcg
      601 atgcgcttgc gctgcggctc ggccgggcga cttcgccggt cattttcgac gcgaagcgcc
      661 tccagggcga tttcgtcggc cgcggagtcg ccggcacttt ctcgggcgcg agctcgacca
      721 tcggcaacgt gccgctggcg atgagcgaca tcgccgggcg ctggcgggtg tacaaaggcg
      781 atttgacgat cgacgggggg atgctggtcg ccgaccgctc gccggcgccg cgcttctacc
      841 cgctgcgcag cgacgacgtc cgcttcagcc tagccggcga cttcgtccgg gcggcgggca
      901 ccctcaagca cccggccagc ggcaccaggg tcaccgacat cgccatcgag caccggctgt
      961 cgaccggggc gggccacgcc ttgctcgacg tccccggcat ccgcttcggc gagaacctcc
     1021 agcccgagga gctgacgcgc ctgaccgagg gcgtcatcgc cctggtccag ggcacggtca
     1081 tcggcaaggg ccggatcgac tggagcagcg aaggcaaggt cacctctacc ggcgagttct
     1141 cgaccgtggg gatggacctc gcggctccgt tcgggccggt caccggcctg acgacgaccg
     1201 ttcgcttcac cgacctgctc gggctcgaga ccgcgcctgg gcagagcgcg acgatcgcct
     1261 cgatcaaccc cggcatcctc gtcgagaacg gggtcatccg ctaccagttg ctgcgaggcc
     1321 agctggtcaa ggtcgagcgc ggcgaatggc cgttcatggg cggccggctg atccttcgcg
     1381 aaacgatcct caatttcggc cggccgagcg ccaagcggct gacgttcgag gtcgtcgggc
     1441 tcgacgccaa gaccttcgtc gacagcctcg gcttctcggg catcgaggcg acaggagtgt
     1501 tcgacggggt gctgccgatg atcttcgacg agagcggcgg ccggatcgtc ggcggccggc
     1561 tcgattcccg cccgggcggc ggcagcctcg cctacaccgg ggtcgtcaac aaggcgaacc
     1621 tggggatgat gggcaatatc gccttcgacg cgctgcgcga cctgcgcttc cgctcgatgg
     1681 tcgtgcgcct cgacggcgag ctcgccggcg agttcacgac ccggctgacg atcgacgggg
     1741 tcgcgctcgg ccagaccggg acgcagaaga tcatccgcgg cctgctgaag aagctgccgc
     1801 tcaagctcaa cgtgtcgatc cgcggcccct tccgggcgct gatcgccacc gccaagtcgt
     1861 tccgcgaccc gcgcaccgtg atccagccgg tgctgccggt gccgctcgac gcgcccggga
     1921 tcgtcaccga ggtccgccgc cacgaggagc aaaagcagca gacccagacc ccgcccgacg
     1981 aggaaatcaa gacgacgccc ccagcaacca ccaacgagag gtgaccatgg cacattcccg
     2041 caagctcagg agaccggcgc tgctcgcggc cgcggtcttt gcgctgccgc tgagcggctg
     2101 catcaccgtc gagccgcccg acaagccgat cgagatcaac ctcaacgtgg ccatccagca
     2161 ggaagtgctg gtgcggctgc agcgcgacgt cgagcaactg atccagcaga acccgcaggc
     2221 gttcccgcag aacccgcccg aaaggccgcg gccgcaatga cgaggctgtg gcccatcctg
     2281 gcggcggcga tggcggccgc ggccgtgcct gcgcccgctc agacgccggc ggtcgacgcg
     2341 gctcgccacg cggggcaggt cggcgagcgg ttcgacggct acatgggctt cgtcgtcgcg
     2401 cctccggcga cggtccggag ccaggtcagc accatcaata tccgccggcg cgcgctgtac
     2461 agcaacctcg ccgcaagcaa gggcgtttcg ccgcaggaag tcggaatcac cgctggctgc
     2521 caattgcttg cccgggtcca ggtcgggcac gcctatatgc tcgtcgacgg acagtggcgg
     2581 cggcggctcg ccggccagcc cgcgccggtc cccgaatatt gccgctgaac gcccccaaat
     2641 ccctccgccg cggttgacta tccgcgaccc ggttcataaa ggggccgcgc ctttggcggg
     2701 ctcaaacgcc gccatgttcc tcgcccttct agcccccaat ccaggccggg gcgacgcaca
     2761 ggggagacgc atggcgacgg acgagaccgg gcaggaccca aagcttccac ccgacgcacg
     2821 tctcgattcg ctcgaggaac ggctcgaacg ggctcaggcg gtggaggcga agcggaccca
     2881 caaggcggag tacaatccga ccgcccggat ttggcagctg atgttcggcc atctggtcgg
     2941 tgcaccggcg ggcggcggga tcatcggctg gggcctggac tcgctgttcg ggacctttcc
     3001 gacgttgttc cttctgatgc tgttcgtcgg gttcggagtc ggggtggtga acgtgatgcg
     3061 gctttcgagg acgcccccgg gcgaaggttc tgaaaagagg tagcgagttc aagtggcggc
     3121 cgagagcaag atcgacccga tgcaccagtt caccgtggag cctctgctcc cgctgaacgt
     3181 gggcggctac gacgtcagct tcaccaacag ctcgctgtgg ctgctgatcg cgctggtcgt
     3241 gatctacggg ttcatggcga tgggcatgag gcgccagctg gtcccgaacc gctggcaggt
     3301 cgcggtcgag ggcctgaccg gcttcattga cgacatggtc aaggtgaaca tcgggcccga
     3361 aggcaagaag ttcgtcccct acatcttctc gctgttcgtg ttcatcctga ccgcgaacct
     3421 gctcgggctg ttcccgctgt cgatcatccc gggccagcac cagttcacga ccaccagcca
     3481 tttcagcatc accggcgtgc tcgccgtgct gagcttctcg atcgtgctga tcgtcggctt
     3541 ctggcgccac ggcctgcatt tcttctcgct gttcgtgccc cacggcacgc ccaaggtgat
     3601 gatgccgctg atcgcggcga tcgagttcat ctcgttcatg gtgcggccgt tcagcctcgg
     3661 cctgcggctg ttcgtggcga tgatcgccgg ccacatcctg atggaagtgt tcgggagctt
     3721 catcgtcacc ggcttcaacg gcggcgcgat gggcgtcggc gtcggcctgc tgagcttcgc
     3781 gttcatcatc ttcgtgaccg cgctcgagct gctggtctgc gccatccagg cttacgtctt
     3841 cgcgcttctg accacgctct atctcaacga cgcgatcaac cttcactaga gtttttcaca
     3901 aaggagtttt cgaaatggac cttacttccg cacaggtgat cggtgccggc ctcgccgcca
     3961 tcggcgtcgg cgccgccgcg atcggcgtcg gcaacgtgtt cggctcgttc ctcgagagcg
     4021 cgcttcgcaa cccggcggcc gccgacggcc agcagggccg cttgtttatc ggcttcgccg
     4081 ccgccgagct gctcgggctg atcgccttcg ccgtggcgat gatcatcctc tacgcgccgc
     4141 ccagcgccta agcacacggg cccacgcgtc gatgccccag atcaaccagc tttcggagat
     4201 cttcttctcg cagctgtttt ggctgctggt ggtgttcggg atcatctatt tcggcatcgc
     4261 ccgggggatg gttccgaaga tccagtcgac ggtcgagctg cgcgagaaga agatcgccga
     4321 cgatctggcg gccgcccagg cggcgcgcgc cgcggccgag gaaaccgagg ccgagtatcg
     4381 cgcgcggatc gacgagagcc gggcgcaggc ggccaagctg acgcgcgagg cgcggcaggc
     4441 gagcgcgctc gagaccgagg ccaaggtccg cgccgcgtcc gagcagatcg ccaggaaggt
     4501 cgcggccgcc gaggcggaga tccgcgccgc cgccgaggcc gcccggaccg aggtcgagtc
     4561 ggtggcggtc gaggcgaccc aggagatggt cgcgctgctg accggggcga aggtcgaccg
     4621 cggccaggcc gccgcggcgg tgaaggcgga actccatggc tgagccgcac cccgagggcg
     4681 tcgcgaccgt cgagcacgat tccggagcgg cccacgccga gccgaccgcg ttcgggctga
     4741 ccgcgccgat gttcatcggc ctggcgatgc tggtcgtgtt cgcgatcctc gtctggaaga
     4801 aggtgccggc ggcgatcggc aaggcgctcg atgccaagat cgcgctgatc cgcgatcagc
     4861 tcgccgaagc cgaggcgctg cgcaaggagg ccgaggcgct caagaaagag tatcaggcca
     4921 aggccaagtc ggccgacgcc gaggccgcgg cggtgatcga gcgcgcccac ggcgaggccg
     4981 gggagatcgt cgccaaggcc aagagcgacg cggaaggcct gatcgagcgc cgcagccgga
     5041 tggccgagga caagatcgcc gccgaggagc gcgccgctat caagcagctt cgcgccgccg
     5101 ccgccgacgc tgcggcgcgg gccgccgccc ggctgatcgc cgagcgcgac gacccggcgg
     5161 ccgacgagca gctcgtcaat cggaccatcg ccgggctggg caaggcgaat tgactgtttc
     5221 ccggcgaagg ccgggatcca ggcttctttt ggttcgctgg ccccggcttt cgccgggagc
     5281 caactactgc cggtgcgagc cgccc
//
LOCUS       DASTZO010000252         3114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_308447,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000252 DASTZO010000000
VERSION     DASTZO010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308447"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(188..514)
                     /locus_tag="VFU20_07085"
     CDS             complement(188..514)
                     /locus_tag="VFU20_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEU5286257.1"
                     /translation="MQDQPAVQNAEPAVAEPEPALPFRSDTILGVCEAVGQDLGFNPL
                     YLRLVFAALFYLNPVMVTGSYLALGAGVAVSRWLYPVPAAAPQALVEAQAHPADNEAE
                     EELLAA"
     gene            complement(703..1335)
                     /locus_tag="VFU20_07090"
     CDS             complement(703..1335)
                     /locus_tag="VFU20_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5286258.1"
                     /translation="MTRILLADDHPMIRTALEALLRDTGYEIVGTAGTGEAALREIER
                     LRPDMLLLDLQMPGGSGIDVLRRIRAGKAAGKPAMKVVLLTAAIDDSTLLEAQSLKVQ
                     GMVLKNSDPAYLLDCLDRIRHGRTWIDPELAGRSKELAKTLAGRGRAPLAPRERQLIG
                     FVRQGLRNRAIAEQLGVTEGTVKTYLHAVFEKLGVRTRTELAMRADEFLA"
     gene            complement(1332..2963)
                     /locus_tag="VFU20_07095"
     CDS             complement(1332..2963)
                     /locus_tag="VFU20_07095"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5286259.1"
                     /translation="MGKGFQLPSGRVIALGRLLLATLFLLAIWVDPSQPAQSAGETYA
                     LLAAYVVFAAAVAALTWNDWWLDAQLAGPSHAIDIVLFTLLVLLTAGYTSPFFTFFVF
                     VLLASAIRWGWRATALTAVLLTLLFVVAGVLVETVTVGFELQRFVVRTGHLVIVSLII
                     IWFGANQWRAHSYRRDEELLARPLLDESPLEIGLRAAMNAVRTSAGTFVWREPGGGRF
                     AGLAVRGGEPTKAEAPGHGLEHSAAPTPFLYDLRRSRALRRDSERNLVEFAPLDAIKP
                     QAASELDLGEGLAIPIRSVGGDGWLFLEAVPSLSTDHIDLGEQIAADIAVHLQRHALL
                     KAAEESAEARSRLTLARDLHDSIVQFLAAAAFRLEAMKRSQASGRELEPELNELKQLM
                     MLEQGELRSFITALRSGPLVAFGDLARDLQALAERLSRQWDVRCVVSAPDAELMIPTR
                     LHLDALQLMREAVANAVRHADATSVRVKLAAVADQLRLDFVNDGAVYPSPGKRLELPQ
                     SLKERVEQAGGELDMARGMGVTKLSISLPIGGRGS"
BASE COUNT          508 a         1045 c         1121 g          440 t
ORIGIN      
        1 cgagctgggc ctcggcaacg ctgaccggag cgttttcgac cgaccggttg accgccattc
       61 ccatcctctc gtctggctcg ccccagggag agccttattc ggaccgtcgt aacccaaggg
      121 cgtaaagacg aggtttatgc ggccttaggc tttcggagag ggaggttaag ggcgttcgga
      181 gcagagatca ggcggcgagc agttcctcct cggcctcgtt gtcggccggg tgagcctgcg
      241 cctcgaccaa tgcctgcggc gccgcggcag ggaccggata gagccagcgc gacacggcga
      301 cgccggcgcc gagcgccaga tagctgccgg tcaccatcac cgggttcagg tagaacaggg
      361 cggcgaagac cagccgcaga tagagcgggt tgaagccgag atcctggccg accgcctcgc
      421 acaccccgag gatggtgtcg cttcgaaacg gcagcgccgg ctccggctcg gcgacggcgg
      481 gctcggcatt ttgaacggcg ggctggtctt gcatcaggaa ctccgttgac gtcgcgggga
      541 cgatcccccg gttgatgcag actgatgagc aagagccgtg ccagaacagg aatataccgc
      601 ttcgccagtg gcttagagcg gttggaaacg ggtccgcccg ggcggacggc cgccgaatgt
      661 tgggaaatgg cgccaatcgc cgggcgccgt ttgtccgggc ggctaagcga ggaattcgtc
      721 ggcgcgcatc gccagctcgg tgcgggttcg aaccccgagc ttctcgaaca ccgcgtggag
      781 ataggtcttg acggtcccct cggtcactcc gagctgctcg gcgatcgccc ggttgcgcag
      841 gccctggcgg acgaacccga tcagctggcg ctcgcgcggc gcaagcggcg cccggccgcg
      901 gccggcgagg gtcttggcca gctccttcga gcggccggcg agctcgggat cgatccacgt
      961 ccggccgtgg cggatccggt cgaggcagtc gagcaggtaa gcagggtccg aattcttgag
     1021 caccatcccc tgcaccttga gcgattgcgc ttcgagcagg gtcgaatcgt cgatcgccgc
     1081 ggtcagcaga acgaccttca tcgccggctt tccagcggcc ttgccggccc ggatccgccg
     1141 aagcacgtcg atgccgctgc cgccgggcat ctgcaggtcg agcagcagca tgtcgggccg
     1201 cagccgctcg atctcgcgca gggccgcctc gccggtgccg gccgtcccga cgatctcata
     1261 tccggtgtcg cgcagcagcg cctcgagcgc ggtccggatc atcggatggt cgtcggccag
     1321 gaggattcga gtcacgagcc cctcccgccg atcggcagag agatcgacag cttggtgacg
     1381 cccatgccgc gggccatgtc gagctcgccg ccggcctgct cgacccgctc cttgagcgac
     1441 tgaggcagct ccagccgctt gccgggcgag gggtagacgg cgccgtcgtt gacgaaatcg
     1501 agccgcagct ggtccgccac cgcggccagc ttgactcgga ccgacgtcgc gtcggcgtga
     1561 cggaccgcgt tggcgaccgc ctcgcgcatc aattgcagcg cgtcgaggtg gaggcgggtc
     1621 gggatcatca gctcggcgtc gggagccgaa accacgcagc gcacgtccca ttgccgcgac
     1681 agccgctcgg cgagcgcctg caggtcgcgg gcgagatcgc cgaacgcgac cagcggcccg
     1741 ctgcgcagcg cggtgatgaa cgagcgcagc tcgccctgct cgagcatcat cagctgcttg
     1801 agttcgttga gctccggctc aagctcgcgg cccgacgctt gcgaccgctt catcgcctcg
     1861 aggcggaacg cggcggcggc gaggaactgg acgatgctgt cgtgaaggtc gcgcgccagg
     1921 gtcagccgcg agcgggcctc ggcgctctcc tcggcggctt tcaggagcgc gtgccgctgg
     1981 agatggacgg cgatgtcggc ggcgatctgc tcgcccaggt cgatgtggtc ggtcgacagg
     2041 ctcgggacgg cctcgaggaa cagccagccg tcgccgccga ccgagcggat cgggatggcc
     2101 agcccttcgc ccagatccag ctcggaggcg gcttgcggct tgatcgcatc gagcggcgcg
     2161 aactcgacca gattgcgctc ggaatcccgc cgcagggcgc ggctcctgcg caaatcgtag
     2221 aggaacgggg tcggagcggc cgaatgctcg agcccgtgac ccggcgcctc ggccttcgtc
     2281 ggctcgccac cccggacggc gaggccggcg aagcggccgc cgcccggctc cctccagacg
     2341 aaggtgccgg cggacgtccg gactgcgttc atcgccgctc tcagcccgat ctcgagcggc
     2401 gattcgtcca gcagcgggcg ggccagcagc tcctcgtcgc ggcggtagga atgcgcgcgc
     2461 cactggttgg ccccgaacca gatgatgatc agcgagacga tcaccaggtg gccggtgcga
     2521 acgacgaagc gctgcagctc gaagccgacg gtgaccgtct ccaccagcac gccggcgacc
     2581 acgaagagca gggtcagaag caccgcggtc aatgccgtcg cccgccagcc ccagcggatc
     2641 gcggaggcca gcaggacgaa gacgaagaag gtgaagaacg ggctggtgta gccggcggtc
     2701 aggagcacca gcagcgtgaa caggacgatg tcgatcgcgt gcgacgggcc ggcgagctgg
     2761 gcgtcgagcc accagtcgtt ccaggtcagg gcggcgaccg cggcggcgaa gaccacataa
     2821 gcggcgagca gcgcgtaggt ttcgcccgcc gactgcgccg gctggctcgg gtcgacccag
     2881 atggcgagca ggaacagggt cgccaggagc agtcggccga gcgcgatgac ccgccccgac
     2941 ggaagctgaa aacccttgcc cacggacccg gtgatatcgc atcgccgccg gaacggacag
     3001 cgggcagggc ggcactgtcc cggccggggc cattgcttcg cgcagcgcgc gccgcggcgg
     3061 gcgcgggccg gcgatactaa ctttcgttga ctggggcgcc ccgcggcggg ctga
//
LOCUS       DASTZO010000253         4256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_282956,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000253 DASTZO010000000
VERSION     DASTZO010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282956"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VFU20_07100"
     CDS             complement(<1..578)
                     /locus_tag="VFU20_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286260.1"
                     /translation="MKYRRLVCALLIVAAPLAFAGASAEPVPKEQLLKPGPAADHYVV
                     VSDAGKHGDMWRWTLPDGRIAFRHSQSLRGWITETDATVRYGEDGRPVEIVVRGITPS
                     GDAGETYTETGGNARWASLVDSGAGPAGKKVYLPAGGPGLVNARLIEHLAAAGDCGIG
                     APLALVAQPPAVAVARSDVHQRAERSALPDRVR"
     gene            complement(654..1049)
                     /gene="ssrA"
                     /locus_tag="VFU20_07105"
     tmRNA           complement(654..1049)
                     /gene="ssrA"
                     /locus_tag="VFU20_07105"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            1155..1445
                     /locus_tag="VFU20_07110"
     CDS             1155..1445
                     /locus_tag="VFU20_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286261.1"
                     /translation="MNKTAFALVGAASIALAGCGGQGDDTLGENVQENLENQAENLDA
                     LAGNAATDAEAEALGNQADQLREEGEEREDAIDDADVNAANPAEADQAVNGM"
     gene            1535..2281
                     /locus_tag="VFU20_07115"
     CDS             1535..2281
                     /locus_tag="VFU20_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5286262.1"
                     /translation="MSDEPIPAATLVVVRESAGGPPELLMVERASGMAFAGGALVFPG
                     GRIDEADRRLAGPFGLADGGARVAAIRETIEETAVAVAVDPLPSVEDALAMQAALVDY
                     RPFEELLDDAGLGLDPGILTPLARWLPNFHVTRRFDTLFYIAPAPPGDWQPSVIAGEC
                     SGAFWLTAAEALERERSGDARLIFPTRRNLERLAQHDSFAAMRADAQAHPVDPITPWV
                     EELAGEKFITIPDDLGYPVTREKLDGLWRG"
     gene            complement(2369..2632)
                     /locus_tag="VFU20_07120"
     CDS             complement(2369..2632)
                     /locus_tag="VFU20_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIB/MDM2 domain-containing protein"
                     /protein_id="HEU5286263.1"
                     /translation="MAKTGRKAGGGLARPVQPSPELGAITGNDPLPRSQVVSKVWDHI
                     RRNNLQNPQNKREIMADDKLRKVFGKDRCSMFEMNKHLSKHLK"
     gene            complement(2707..3519)
                     /locus_tag="VFU20_07125"
     CDS             complement(2707..3519)
                     /locus_tag="VFU20_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEU5286264.1"
                     /translation="MKIAVVAPSCTLTREAAERVQALADARGDCELAFHPQCFRSDGH
                     FAGSDGERLAALREVMADRSVDTVWFARGGYGSNRIAEAALADLPEAARSKLYMGYSD
                     PGFLLAGLHRAGLEVAHGPMPRDVLRDGGEGAVTRALDWLVRRDPSALEPDLEGPAFA
                     FNLTVLSCLLGTPLEPDLSGAELLIEEVGEQHYRIDRAMFHVTASAKVRGVARIRLGR
                     VCDILPNDPEFGRDELAIVEDWCARAGIPYGGRADIGHDASNRVVPFPSKIS"
     gene            3567..>4256
                     /locus_tag="VFU20_07130"
     CDS             3567..>4256
                     /locus_tag="VFU20_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HEU5286265.1"
                     /translation="MIDSTFFCGIGGSGMLPLACIVRASGGRVAGSDRALDAGRVAPK
                     FAYLRSLGITLFPQDGSGLQPGMTFVTSAAVEDTVPDVVRARELGLTHLTRPQFLARL
                     LNAAQRSVAVGGTSGKSTVTGMIGWILHACHRQPTVMNGAVMKNFATPSAPFASALVG
                     DPELFVSEVDESDGSIALYRPEVAVLTNISLDHKEMAELRSLFAAFLLRARKAVLNLD
                     DPETRAIADVVP"
BASE COUNT          642 a         1486 c         1444 g          684 t
ORIGIN      
        1 cgcacgcgat ctgggagagc ggagcgctcg gcccgctgat gtacatccga gcgcgctacg
       61 gccacggcgg gcggctgggc tacgagcgcg agtggcgcgc cgatcccgca atcgccggcg
      121 gcggcgagat gctcgatcag ccgcgcgttg acgaggcccg ggccgccggc gggaagatag
      181 accttcttgc ccgccgggcc ggcgccggaa tccaccaggc tggcccagcg cgcgttgccg
      241 cccgtctccg tgtaggtctc gccggcgtcg cccgacgggg tgatcccgcg cacgacgatc
      301 tcgaccgggc ggccatcctc gccgtagcgg acggtcgcgt cggtctcggt gatccagccg
      361 cgcagcgact gcgagtggcg gaaggcgatg cgcccgtccg gcagggtcca gcgccacatg
      421 tcgccatgct tgccggcgtc cgacacgacg acataatggt cggccgccgg accgggcttg
      481 agcagctgct ccttgggcac cggctcggcg gaagcgccgg cgaacgcaag cggcgcggcg
      541 acgatcagca gcgcgcaaac aagacgacga tatttcattc gggactcccc tggaaacggc
      601 ggggagactg ggcgagccga aaccggttgg caaggtttcg cagggcccgc gaaagtggga
      661 ggcttctgtt gcccggtgcc tcccgaaccg ctggaatccc cttctgttgc ccggtggggt
      721 ccgaccgcgc ttttgtcttt gtaacttcag gccgctaggc cgtcagttac gcagcaattg
      781 cgagcgcctc gttatctttg gcacttgtgg ttatgaacgg ttttacggct cattcaggcc
      841 gaccggcaat caggccttta ctgcactcgt cgatcctatt tcgcccccgt cagcaaccgc
      901 cggttttcgc ccggttggcg gctggtggtg gaggcgccgg ggtactgccc cccgggtccg
      961 aaatgcctat tccgcctaac actctaccga catagccggg tcgccccggc gcatcctaaa
     1021 taggcgtttt ggcacggatt ctcaaggtcg ggccggttcg tccgccagcc gaaccgcatg
     1081 cagccactgg aatttcgacc gccgctgctg ttatgcgcga agccgggaac agccgaaccg
     1141 aaagggaaac ttgaatgaac aagactgcat tcgcgctggt cggcgcggcc tcgatcgcgc
     1201 tcgccggatg cggcggccag ggcgacgaca cgctgggcga gaacgtccag gagaatctgg
     1261 aaaaccaggc cgagaatttg gacgcgctgg ccggcaacgc ggccaccgac gccgaggccg
     1321 aagcgctcgg caaccaggcc gaccagctgc gcgaggaagg cgaggagcgc gaggacgcga
     1381 tcgacgacgc cgacgtcaac gccgccaacc cggccgaagc cgaccaggcc gtcaacggca
     1441 tgtaggctgg cgcggggttg aaggaatccc ggggccgcgg gcgcgacacc cgcggccctt
     1501 tttctttgcg cggcgctggc ctagcgcaac cgcgatgagc gacgagccga tccccgcggc
     1561 gaccctggtc gtggtgcgcg agagcgcggg cggcccgccc gagctgctga tggtcgagcg
     1621 cgcgagcggc atggcgttcg ccggcggcgc gctggtgttt cccggcggcc gcatcgacga
     1681 ggccgaccgc cgcctagccg ggccgttcgg cctggccgac ggcggcgcgc gggtcgcggc
     1741 gatccgcgag acgatcgagg agaccgcggt cgcggtcgcg gtcgacccgc tgccctcggt
     1801 cgaggacgcg ctggccatgc aggccgcgct ggtcgactac cggccgttcg aggagctgct
     1861 cgacgatgcc gggctcggcc tcgacccggg catcctgacg ccgctcgccc gctggctgcc
     1921 caacttccat gtcacccggc gcttcgacac cctgttctac atcgcgcccg ccccgcccgg
     1981 cgactggcaa ccgagcgtca tcgccggcga gtgcagcggc gccttctggc tcaccgcggc
     2041 cgaggctctg gagcgcgagc gaagcggcga cgcccggctg atcttcccga cgcggcgcaa
     2101 cctcgagcgg ctcgcgcagc acgacagctt cgccgcgatg cgcgccgacg cgcaggcgca
     2161 tccggtcgat ccgatcactc cgtgggtcga ggagctggcc ggggaaaagt tcatcaccat
     2221 tcccgacgac ctcggctatc cggtcacccg cgagaagctc gacggcctct ggcgcggctg
     2281 aacgaaatca ggcgattcgc gctctcccgg tcatgaaaaa ggggacccgc tcgcgcggat
     2341 cccctctcgg acaagccttt cggccggtct atttcagatg cttggagaga tgcttgttca
     2401 tctcgaacat gctgcagcga tcctttccga agaccttgcg cagcttgtcg tcggccatga
     2461 tttctctttt gttttgcggg ttttgcaggt tgttcctgcg gatgtgatcc cacaccttgg
     2521 aaaccacctg gctgcgcgga agcggatcat tgcccgtgat cgctccgagt tccggtgatg
     2581 gttgaaccgg gcgagccagt ccgccccctg ctttcctgcc tgttttcgcc atgagcgccc
     2641 tcctgttgtt gtcctctttt gtagagcgca aaggcgtctc ctttgcaatg gcctgttaga
     2701 ggcggatcag cttattttgg acggaaacgg aacgacccgg ttcgacgcgt cgtggccgat
     2761 gtcggcgcgg ccgccgtacg ggatgccggc gcgcgcgcac caatcctcga cgatcgccag
     2821 ctcgtcgcgg ccgaactcgg ggtcgttggg aaggatgtcg cagacccgcc cgaggcgaat
     2881 gcgggcaacg ccgcggacct tggcgctcgc ggtcacatgg aacatcgcgc ggtcgatgcg
     2941 ataatgttgc tcgccgacct cctcgatcag cagctcggct cccgacaggt ccggttccag
     3001 cggcgtcccg agcaggcacg acagcaccgt caggttgaac gcgaacgcgg gcccttccag
     3061 gtccggttcg agcgcggacg ggtcccgccg caccagccag tcgagcgccc gcgtcaccgc
     3121 gccctcgccg ccgtcgcgca gcacgtcgcg gggcatggga ccgtgcgcga cctcgagccc
     3181 ggcgcggtgc aggccggcga gcaggaagcc gggatcgctg tagcccatgt agagcttcga
     3241 ccgcgccgct tcgggcaggt cggcgagcgc ggcttcggcg atccggttcg acccgtagcc
     3301 gccgcgcgcg aaccacaccg tgtcgaccga tcggtcggcc atgacttcgc gcagcgccgc
     3361 cagccgctcc ccgtcgctgc ccgcgaaatg gccgtccgac cggaagcatt gcgggtggaa
     3421 ggcgagctcg cagtcgccgc gggcatcagc gagcgcctgc acccgctcgg ccgcctcgcg
     3481 cgtcagggtg cagctcggag cgaccaccgc gatcttcatt cgtcccccat ttgtcatgcg
     3541 gccccgcgtt caatagggca ccgccgatga ttgattccac cttcttctgc gggatcggcg
     3601 gcagcggcat gctgccgctg gcctgcatcg tccgggcgtc gggcgggcgc gtggccggct
     3661 ccgaccgggc gctcgacgcg ggccgcgtcg cacccaagtt cgcttatctg cgctcgctcg
     3721 gcatcacgtt gttcccccag gacggctccg ggctccagcc gggcatgacc ttcgtgacct
     3781 cggccgcggt cgaggacacg gtccccgacg tcgtccgcgc gcgcgagctc gggctcaccc
     3841 acctgacccg gccgcaattc ctcgcccggc tgctcaacgc cgcgcagcgc agcgtcgccg
     3901 tcggcggcac cagcggcaag tcgaccgtca ccgggatgat cggctggatc ctccacgcgt
     3961 gccaccgcca gccgacggtg atgaacggcg cggtgatgaa gaatttcgcg acgccctcgg
     4021 cgccgttcgc gagcgccctg gtcggcgacc ccgagctgtt cgtcagcgag gtcgacgaga
     4081 gcgacggatc gatcgcgctt taccggcccg aggtcgcggt tctcaccaac atcagcctcg
     4141 accacaagga gatggccgag ctgcgcagcc tgttcgcggc gttcctgctg cgggcgcgga
     4201 aggcagtgct caatctcgat gatccggaga cgcgcgcgat cgccgatgtc gtcccg
//
LOCUS       DASTZO010000254         3044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_21682,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000254 DASTZO010000000
VERSION     DASTZO010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21682"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..726
                     /locus_tag="VFU20_07135"
     CDS             286..726
                     /locus_tag="VFU20_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286266.1"
                     /translation="MRKSMLAALGAATMLLAMPAAAQDSPLKSGNFWNVAEITIDDGH
                     FIDYADHLAGLYRKSQDFAKSKGWIKESIILANINKRAGEPDLYLVTISDRITTPAED
                     EAREKEMNAYLATNARKQDEQSGARAKYRKLGGNMLLQELLYKR"
     gene            complement(781..1230)
                     /locus_tag="VFU20_07140"
     CDS             complement(781..1230)
                     /locus_tag="VFU20_07140"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5286267.1"
                     /translation="MVKTAFVISAAALLLGSAAQSQVNPAGLRWGPAPPSLPRGAQLA
                     VLAGDPQSAGMFTIRLRFPPGYSVAPHSHPTDEFVTVISGQMGLGMGSRMDRARATRL
                     VPGGFVIAPANMNHYAFTRTGAVIQITAHGPFAVTYVNPADDPRLRR"
     gene            complement(1278..1949)
                     /locus_tag="VFU20_07145"
     CDS             complement(1278..1949)
                     /locus_tag="VFU20_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEU5286268.1"
                     /translation="MAKNTSHGSNAVVVIGLGRFGGSVAQSLARLGHEVLGIDFSADR
                     VQELADRLTHVVEADSTHADTLRRLGVHEFEHAVVGIGSDVEASVLTVLALDELEIPD
                     IWAKAVSDQHGQILEKTGAHHVVYPEATMGERVAHLVTGKMIDFMEFDDGFAIVKTRT
                     PEEAVGKSLGESALRSKYGITIVGVKRPHTDFTYATPDTVVERGDLLIVSGPTKRVEK
                     FAAVT"
     gene            complement(1942..>3044)
                     /locus_tag="VFU20_07150"
     CDS             complement(1942..>3044)
                     /locus_tag="VFU20_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011444059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HEU5286269.1"
                     /translation="IQVGGFGIMTGATMLGLLVTRRLKLNYRLLTQAETRTFTLGDVT
                     SVLRLILIVTLTVELLVAAVLTSRFLLHYEQPLGSALWNGLFHAVSAFNNAGFARFSD
                     SVMGFALDPYVLVPLTLAVVLGGLGFPVLYELRGELLTPATWSLHTKITLLGTAILLA
                     GGFLFVLAFEWRNPGTIGPMDSGGKLLSALFHSVVTRTAGFNSLDIAQMRPETLLVSD
                     ALMMIGGGSAGTAGGIKVTTFLVLGLIVWAEVRGEPDASAFGRRLSSEVQRQALTVVL
                     LAIGVVAAGTLVLLSVSRFGLSEVLFEAVSAFATVGLSTGITAQFPPVGQVTLILLMF
                     IGRVGTITVATALALRGSRRPYRYPQERPIVG"
BASE COUNT          549 a          996 c         1062 g          437 t
ORIGIN      
        1 tcattcctcc agatgggcgg cggccggcgt accggcgagc tggggccgcc gggcaagagc
       61 acgggaccga tcgccggcga gccgaggcgc aagttcggcg cctagccggg ccgcggcaga
      121 tccggctggc ccggtcggcg caacccgagg atttgtcgga tttgcttagg aaaagccggc
      181 cggccgtgct taggctggcg ggcagcgccg ccggacgatc cgtctcgacc gctgacgcgc
      241 cttgacgcgc gcgggccctg tggcccggat tgggaaggaa caatcatgcg aaagtccatg
      301 cttgccgcgc tcggcgcggc gacgatgctg ttggccatgc cggcagccgc ccaggacagc
      361 ccgttgaaaa gcggcaattt ctggaatgtc gcggagatca cgatcgatga cggccacttc
      421 atcgattatg ccgaccacct cgccggactg taccgcaagt cgcaggactt cgcgaagtcg
      481 aagggctgga tcaaggagtc gatcatcctc gccaacatca acaagcgggc cggcgagccc
      541 gacctctatt tggtgacgat cagcgaccgc atcaccactc cggccgagga cgaagcgcgc
      601 gaaaaggaga tgaacgccta tctcgcgacc aacgcccgca agcaggacga gcagagcggg
      661 gcgcgggcca agtaccgcaa gctcggcggc aacatgctgc tccaggaact gctctacaag
      721 cgctagacga cggatgctcc gggcagcgca gggctgcccg gaccagccgc tgcgacggct
      781 ctacctgcgc aggcgcgggt cgtcggcggg gttgacgtag gtgaccgcga acggcccgtg
      841 ggcggtgatc tggatcaccg cgccggtgcg ggtgaacgca taatggttca tgttcgccgg
      901 agcgatcacg aaaccgccgg gaaccagccg ggtcgcccgg gcgcggtcca tgcgcgagcc
      961 catgcccagc cccatctgtc cggaaatcac cgtcacgaac tcgtcggtcg ggtggctgtg
     1021 cggggccacc gaatagccgg gcgggaagcg cagccggatg gtgaacatgc cggccgactg
     1081 cgggtcgccg gcgagcaccg cgagctgcgc cccgcgcggc agcgacggcg gcgccgggcc
     1141 ccatctgagg ccggccggat tgacctgcga ctgcgccgcc gagccgagca acagagccgc
     1201 ggcggaaatc acgaatgccg tcttgaccat cgtccttctc cccttttgcg tccgcccccc
     1261 ggccggcgtg tagcacttca ggtcacggcc gcgaacttct cgacccgctt ggtcgggccg
     1321 gagacgatca ggaggtcgcc cctttcgacc accgtgtccg gggtcgcgta ggtgaaatcg
     1381 gtgtgcggcc gcttcacccc gacgatggtg atcccgtact tgctgcgcag tgccgattcg
     1441 ccgagcgact tgccgaccgc ttcctcgggc gtgcgagtct tgacgatcgc gaagccgtcg
     1501 tcgaactcca tgaagtcgat catcttgccg gtgacgaggt gggcgacgcg ctcgcccatc
     1561 gtcgcttcgg gatagacgac gtggtgcgcg ccggtcttct cgaggatctg gccgtgctgg
     1621 tcgctgaccg ccttggccca gatgtcgggg atctccagct cgtcgagcgc gagcacggtg
     1681 aggacgctcg cctcgacatc gcttccgatg ccgaccaccg catgctcgaa ctcgtggacg
     1741 cccagccggc gcaacgtgtc ggcatgggtt gaatccgcct cgacgacgtg ggtcagacgg
     1801 tcggccaact cctgaacccg gtcggcgctg aaatcgatcc cgaggacttc atggccgagg
     1861 cgcgcgaggg attgcgcgac cgagccgcca aagcggccaa ggccgatgac gacgaccgcg
     1921 ttgcttccgt gcgatgtgtt cttagccaac gattgggcgc tcctgtggat agcggtacgg
     1981 ccgccgcgac ccgcgcaagg cgagcgcggt tgcgacggtg atcgttccaa ccctgccaat
     2041 gaacatcagc aggatcaaag tcacctggcc gaccggcggg aattgcgccg tgatcccggt
     2101 cgacaggccg actgtggcga acgccgacac cgcctcgaac aggacctcgg acaggccgaa
     2161 gcgcgagacg ctgagcagga ccagggtgcc cgccgcgacc accccaatgg cgagcaggac
     2221 gacggtcagc gcttggcgct ggacttcgga cgacagccgg cggccgaacg ccgaggcgtc
     2281 gggctcgccg cgcacctcgg cccagacgat gaggccaagg acgaggaagg tggtgacctt
     2341 gatgccgccc gcggtcccgg cgctgccgcc gccgatcatc atcagagcgt cgctgaccag
     2401 cagggtctcg ggccgcatct gggcgatgtc gaggctgttg aagccggcgg tccgggtgac
     2461 gaccgaatgg aacagcgccg agagcaattt gccgcccgaa tccatcggcc cgatggtgcc
     2521 cgggttgcgc cattcgaacg cgagcacgaa caggaagccg ccggccagca ggatggcggt
     2581 tccgagcaaa gtgatcttgg tgtggagcga ccatgtggcg ggcgtgagga gctcgccgcg
     2641 aagctcgtag aggaccggga atccgaggcc gccgagcacc accgcgagcg tcagcgggac
     2701 gaggacatac gggtcgagcg cgaagcccat cacactgtcc gaaaagcgcg cgaagccggc
     2761 attgttgaac gccgagaccg catggaacag gccattccac agcgccgagc ccagcggctg
     2821 ttcgtagtgc agcaggaagc gcgaggtcag caccgcggcg acgagcagct cgacggtcag
     2881 ggtgacgatc aggatcaggc gcagcaccga ggtcacgtcg ccgagcgtga aggtgcgggt
     2941 ctcggcctgg gtgaggagcc ggtagttgag cttgagccgg cgggtcacca gcagcccgag
     3001 catcgtcgcc ccggtcatga tcccgaagcc gccgacctgg atca
//
LOCUS       DASTZO010000255         5257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_98922,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000255 DASTZO010000000
VERSION     DASTZO010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98922"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1396)
                     /locus_tag="VFU20_07155"
     CDS             complement(<1..1396)
                     /locus_tag="VFU20_07155"
                     /inference="COORDINATES: protein motif:HMM:NF024994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5286270.1"
                     /translation="MTAPRNAVLVGASDRPGSWAAKVWENLNTYEFPGPIYLINPRRT
                     EIWGRPCYADFSALPEKPDHMVIMVPAAGVIDALKSGAAAGARSATVFSAGFGEGFNT
                     EAAALGRELAEAIAQTGLAVSGPNCMGNVCSKSRLVTLTEDRPLTVRPGPVALVGQSG
                     GMMIYSNQALLERGIAPGYLITSGNEAGLSLADYIAFFAGEAEIKVIIIYIEAISDVA
                     KFKAACRLARASGKSVVAIKLGLSEAGRHAALAHTGSLAGSVEAFDAIAGEAGVIRAE
                     TLDDAVEITELLVHTGAPTGRRLGAVTLSGAFRGMLLDAAERYGLEFRPLAPETTERL
                     NAVLTVGSLVSNPIDGGFGVLSSADNFMASIDALHNDPNVDMVIVQEAPPRAPGSDRS
                     EHYIRLANDYAATKATKPIAFMSPITLGHTDYSRGVRAEAPHLSFLQEAYKGLRAIAT
                     VARRAEHERLAGSEA"
     gene            complement(1551..2942)
                     /locus_tag="VFU20_07160"
     CDS             complement(1551..2942)
                     /locus_tag="VFU20_07160"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5286271.1"
                     /translation="MTATSDQTGGEALPRDRHLFGPGPKRILSLDGGGVRGAISVAFL
                     ERIEKIIQEEHARTNGNGAAPPRLGDYFDIVGGTSTGAVIAGALALGKNTSEIKDFYL
                     KLAPRIFSRPFWRVPGLLAKFDAKALREEIDNIVKDRRLDSPDLITGLCVVTKRLDTG
                     SPWILANNSRAPYWETTPSDPVNNRKGHTGNREYRLANLVRASTAAPHFFDPEIIAIN
                     EDERKQPLADANARLAGMPWLSLLVSKLRLLLLEAGHGARGKNIADTHGIFVDGGVTP
                     FNNPTMALLMMTQLKGFGLEWPLGPKNLSIVSIGTGSSRVSLSFKELGIFGPLRVTLS
                     ALLSLMSDVQTLSLAQMQWLGECPDPWEINSEIGQLAGDAPPGGKWFRFLRYDVRLDP
                     VWLKQNLQLDFTDREVDKFRKMDDPGIIKNIYAIARLAAELQVKREHFFPNVAPASAA
                     GVQAPHSLPLPTG"
     gene            complement(2959..3858)
                     /locus_tag="VFU20_07165"
     CDS             complement(2959..3858)
                     /locus_tag="VFU20_07165"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5286272.1"
                     /translation="MTETAVTIPSAGLKLSGVVHVPDDVRPGERRPAFMVLHGFGSNK
                     SSSNTMEPTRILNRMGYVVLRFDMRGCGESEGERGNLICLEQVEDTSNALTFLTKHPA
                     VDPNRIAVVGSSFGGAVAVYTGGVDPRVAAVISNGGWGDGERKFRGQHPTPEAWKKFT
                     DMLALGRGYRAKHGKPLMVPRYDIVPIPPHLRTNLAKDSIHEFTSETAQSMYDFRADD
                     VVGKIAPRPLLLLHSSNDSVTPTEQSIEMFKRAGHPTELHLIADVDHFMFAEGNKRVW
                     DLVRNWLNDYFPVVPAARTHAAE"
     gene            complement(4016..>5257)
                     /locus_tag="VFU20_07170"
     CDS             complement(4016..>5257)
                     /locus_tag="VFU20_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5286273.1"
                     /translation="VADVNQKLGKSVRDAEISALQPPPIDNLGNSSGFSFRLQDRAQK
                     GYAELMRAKDQLLAAAARSPILQDVYVEGLAPSPQVELNIDREKASAFGVTFEEINNT
                     ISTNLGSAYINDFPNRGRMQRVVVMADRSSRMQADSILSYNVRNTQGKLVPLSSFATV
                     DWSVGPAQIVGFNYYMSVRISGSAKPGYTSGDAINEMERLARQLPRGFGFDWTGQSLQ
                     EKQSGAQAPLLLALSALLVFLVLAALYESWTIPIAVLLTVPLGMLGAALAAMGRGLPN
                     DVYFTVALVTIIGLAAKDGILIIEFAKALREQGKSLKDATLEACHLRFRPILMTGLAF
                     CFGVAPMVIASGASAKSQQAIGTSVMGGMIAVVVLALAFVPVFFVAVLKSAEWIRTRK
                     ERRPVADAERSEATVAPEHQA"
BASE COUNT          843 a         1694 c         1821 g          899 t
ORIGIN      
        1 cagcctcgct gccggcaagc cgttcgtgct cggcacggcg cgcgacggtg gcgatggcgc
       61 gcagcccctt ataggcctct tgcaggaatg aaaggtgcgg cgcctcggcg cgaacgccgc
      121 ggctgtagtc ggtatggccg agcgtgatcg gcgacatgaa ggcgatcggc ttggtcgcct
      181 tggtggcggc gtagtcgttg gcgagccgga tgtaatgctc gctgcggtcg gagccgggag
      241 cgcgcggcgg cgcctcctgg acgatcacca tgtcgacgtt cggatcgttg tggagcgcat
      301 cgatcgacgc cataaaattg tccgcgctgc tgagcacgcc gaagccgccg tcgatcggat
      361 tgctgaccag cgatccgacc gtcaggacgg cattgagtcg ctcggtggtt tccggtgcga
      421 gcggccggaa ttccaggccg tagcgctccg cggcgtcgag cagcataccg cgaaatgcac
      481 cggacagcgt cacggcgccg agccggcggc cggtgggcgc gccggtatgg acgagcagtt
      541 cggtgatctc gaccgcatcg tcgagcgtct cggcgcggat cacgcccgct tcgccggcga
      601 tggcgtcgaa cgcctcgacc gagccggcga gcgagccggt atgcgccagt gcggcatggc
      661 ggccggcctc cgacaggccg agcttgatcg ccaccaccga cttgccggag gcacgcgcca
      721 gccggcaagc cgctttgaat ttcgcgacgt ccgagatcgc ctcgatgtag atgatgatga
      781 ccttgatctc ggcctcgccg gcgaagaacg cgatgtaatc ggcgagcgac aggccggctt
      841 cgttgccgct ggtgatcaag tagcccggcg cgatgccgcg ctccagcagc gcctggttgg
      901 agtagatcat catgccgccg ctctggccga ccagcgccac cggtccgggg cggactgtaa
      961 gcggccggtc ctcggtcagc gtcacgagcc ggctcttgga gcagacgttg cccatgcagt
     1021 tcggccccga caccgccagg ccggtctgtg cgatcgcctc ggcaagctcg cggccgagcg
     1081 ccgcggcctc ggtgttgaag ccttcgccga agccggccga gaataccgtc gcgctgcgcg
     1141 cgccggcggc ggcgccgctc ttgagcgcgt cgatcacgcc ggcggccggc accatgatca
     1201 ccatgtggtc gggcttctcg ggcagcgccg aaaaatccgc atagcagggt cggccccaga
     1261 tctcggtgcg gcggggattg atcaggtaga tcgggcccgg aaactcgtag gtgttgaggt
     1321 tctcccacac cttcgcggcc cagctcccgg gacggtcgct ggcgccgacc agcacggcgt
     1381 tgcggggcgc ggtcaaagcc gcgacttttt ctctggaggt ttcgaggtcc aacgggctcc
     1441 cctttttttg ccgtcaggcg gccgggccaa gctcaatagc atgtcggcgc tcgaagcgag
     1501 agatggggca gcgggatgcg ggaaccgcct gagcgcaatg ctcggcgcgg ctaaccggtc
     1561 ggcaggggca gcgaatgggg cgcttgaacg ccggctgcgc tcgcgggtgc gacgttgggg
     1621 aagaaatgct cgcgcttcac ttgcagctcg gcggcaagtc gcgcgatggc atagatgttc
     1681 ttgatgatcc ccggatcgtc catcttgcgg aacttgtcca cctcgcggtc ggtgaaatcg
     1741 agctgcaggt tctgcttcag ccagaccgga tcgagccgca cgtcgtagcg caggaaacgg
     1801 aaccacttgc cgccgggcgg cgcgtcgccg gcgagttgcc cgatttcgga attgatctcc
     1861 caggggtcgg ggcattcgcc gagccactgc atctgggcga gcgacagcgt ctggacgtca
     1921 ctcatcagcg acagcagcgc gctcagcgtc acccggagcg ggccgaagat tcccagctcc
     1981 ttgaacgaga gactgacgcg gctcgagccg gtgccgatcg aaacgatcga caggtttttc
     2041 ggtccgagcg gccattcgag cccgaatccc ttgagctgcg tcatcatcag caacgccatg
     2101 gtgggattgt tgaacggcgt tacgccgccg tcgacgaaga tcccatgggt atcggcgatg
     2161 ttcttgcccc tggcgccgtg gcccgcctcc aacagcagca ggcggagctt gctgaccagg
     2221 agcgacagcc atggcatgcc ggcgagccgg gcattggcgt cggccagcgg ctgcttgcgc
     2281 tcgtcctcgt tgatcgcgat gatctccgga tcgaagaaat gcggcgcggc ggtgctggcg
     2341 cgtaccagat tggcgagccg gtactcgcgg ttgccggtat ggcctttgcg gttgttgacc
     2401 ggatcggaag gcgtcgtctc ccagtatggc gcgcgcgagt tgttggcgag gatccagggg
     2461 ctgccggtat cgagccgctt ggtcacgacg cagagaccgg tgatcaggtc cgggctgtcg
     2521 agccggcgat ccttgacgat gttgtcgatc tcttcgcgca acgccttggc gtcgaacttc
     2581 gccagcaggc caggcacccg ccagaacggc cgcgagaaga tacgcggcgc gagcttcagg
     2641 tagaaatcct tgatctccga ggtgttcttg ccgagcgcaa gcgcgccggc gatcaccgcc
     2701 ccggtcgacg tgccgccgac gatatcgaag tagtcgccga gccgcggcgg cgcggcgccg
     2761 ttgccgttgg tccgggcgtg ctcctcctgg atgatcttct cgatgcgctc caggaacgcg
     2821 accgagatcg cgccgcgcac gccgccgccg tcgagcgaca gaatccgttt cggacctggg
     2881 ccgaacagat ggcggtcgcg cggcagcgcc tcgccgccgg tctggtcgga tgtcgcggtc
     2941 acggtcgcgc cgccatgatc attcggcggc gtgcgtccga gccgccggaa cgaccgggaa
     3001 gtagtcgttg agccagttgc ggaccagatc ccagacgcgc ttgttgcctt ccgcgaacat
     3061 gaagtggtcg acgtcggcga tcaggtgcag ttcggtcgga tggccggcgc gcttgaacat
     3121 ctcgatcgac tgctcggtcg gggtcaccga gtcgttcgac gaatgtagca gcaggagcgg
     3181 ccggggcgcg atcttgccga ccacatcgtc ggcgcggaag tcatacatgc tctgcgcggt
     3241 ctcggacgtg aattcgtgga tcgagtcctt cgccaagttg gtgcgcaggt gcggcgggat
     3301 cggcacgatg tcgtagcgcg gcaccatcag cggcttgccg tgcttggcgc gatagccgcg
     3361 gccgagcgcg agcatgtcgg tgaacttctt ccaggcttcg ggggtcggat gctggccgcg
     3421 gaacttgcgc tcgccgtcgc cccagccgcc gttcgagatc accgccgcga cgcgcggatc
     3481 gacgccgccg gtatagaccg cgaccgctcc gccgaagctc gaaccgacta ccgcgatgcg
     3541 gttgggatcg accgccgggt gcttggtcag gaacgtcagc gcgttgctgg tgtcctcgac
     3601 ctgctccagg cagatcaggt tgccgcgctc gccctcgctc tcgccgcagc cgcgcatgtc
     3661 gaaccgcagc accacgtagc ccatccggtt caggatcctg gtgggctcca tggtgttgga
     3721 cgagcttttg ttgctgccaa agccgtgcag caccatgaag gcgggacggc gctcgcccgg
     3781 ccgcacgtcg tccggcacat gcacgacacc ggagagcttg agcccggcgg acggaatggt
     3841 gacggcggtt tcggtcatcg gcaggtttcc ttgttgttct caacggaatg gcggggccgt
     3901 cgcggcgctt gtgagcgcgc catggacggc cgagggggac tttatcgcaa tggcgtcgcg
     3961 agggaagatg gcacggcgcc ggtccgcggc gaaagcccaa caaacttggc ggcgatcagg
     4021 cctgatgctc gggtgcgacc gtggcctcgg agcgctcggc atccgcaacc ggccggcgct
     4081 ctttccgggt ccggatccat tcggcggatt tcagcaccgc cacaaagaac accgggacga
     4141 aggcaagcgc caggacgaca accgcgatca tcccgcccat gacgctggtg ccgatcgcct
     4201 gctggctctt ggcgctggcg cccgacgcga tgaccatcgg cgcgacgccg aagcagaacg
     4261 cgaggccggt catcaggatc ggccggaagc gcaggtggca ggcttccagc gtcgcatcct
     4321 tcaggctctt gccctgctcg cgcagcgctt tggcgaattc gatgatcagg atgccgtcct
     4381 tggcggcgag gccgatgatc gtcaccagcg ccacggtgaa atagacgtcg ttcggcagcc
     4441 cccgccccat cgcggcgagc gccgcgccga gcatgccgag cggcacggtc agcagcacag
     4501 cgatcggaat ggtccagctc tcgtacagcg ccgccagcac caggaacacc agcagcgccg
     4561 acagcgccag cagcagcggc gcctgcgcgc ccgactgctt ctcctggagc gactggccgg
     4621 tccaatcgaa gccgaatccg cgcggcagtt gccttgcgag ccgctccatc tcgttgatcg
     4681 cgtcgcccga ggtgtagccc ggcttggcgg agccgctgat gcgcaccgac atgtagtagt
     4741 tgaatccgac gatctgcgcc ggcccgaccg accagtcgac cgtcgcgaac gaggacagcg
     4801 gcacgagctt tccctgcgtg ttgcgcacgt tgtagctcag gatgctgtcg gcctgcattc
     4861 gcgaggaccg gtcggccatc acgacgacgc gctgcatccg cccgcgattc gggaaatcgt
     4921 tgatgtaggc cgagccgagg ttggtcgaga tcgtgttgtt gatctcctcg aaggtcacgc
     4981 cgaaagccga tgccttttca cggtcgatgt tcaattcgac ctgcggcgag ggcgcgaggc
     5041 cctcgacgta gacatcctgc aagatcgggc tgcgcgccgc ggcggccagc agctgatcct
     5101 tcgcgcgcat cagttcggca tagcccttct gggcgcgatc ctggaggcgg aagctgaagc
     5161 ccgacgagtt gccgagattg tcgatcggcg gcggctgcag cgccgagatc tcggcgtcgc
     5221 ggaccgactt gccgagcttc tggttcacgt cggcgac
//
LOCUS       DASTZO010000256         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_229473,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000256 DASTZO010000000
VERSION     DASTZO010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_229473"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="VFU20_07175"
     CDS             complement(<1..693)
                     /locus_tag="VFU20_07175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014076580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HEU5286274.1"
                     /translation="MNLIAPIAEGRGRLRPRSSRPDETLPLDRIIEDDCIAAMARLPD
                     KSIDMIFADPPYNLQLGGDLFRPEGSRVDAVDDEWDKFDNIAHYDHFTRRWLGEARRI
                     LKDDGSIWVIGSYHNIFRVGALLQDAGYWILNDIVWRKSNPMPNFRGTRFTNAHETLI
                     WCAKDEKARYTFNYRSMKALNDDLQMRSDWVLPICSGQERAKDEDGLKAHPTQKPEAL
                     LYRILLACTKPGD"
     gene            complement(841..1509)
                     /locus_tag="VFU20_07180"
     CDS             complement(841..1509)
                     /locus_tag="VFU20_07180"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU5286275.1"
                     /translation="MGRPCFKIELDYPEPLAGVDEAGCAPLAGPVVAAAVILNRNCFP
                     RGIDDSKNLPIEKREAIHARLVKCAVIGVGVASVEEIDTLNIYWARMLAMTRAVEALG
                     VDPAWVLVDGNACPRWQRPSKAIVAGDARCRSIAAASIVAKVTRDRMMAERARDYPGY
                     GWETNRGYGTPEHYRGLETLGVTPLHRRSFAPVREIIARHAQPKLPLSESIPASPLSH
                     TTIA"
     gene            1508..1963
                     /locus_tag="VFU20_07185"
     CDS             1508..1963
                     /locus_tag="VFU20_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286276.1"
                     /translation="MDRQLIAGRGEPPDGRSKICGLDSGRPADFAERGNPGRKTVPYL
                     IPAVLSALALAVAAIGFRSAHRFKRPALFKTGAIVAAVGLMLLWLLPWWAIARDAGAW
                     AMPLMVAALVVAVSVLGAGLSTMARAATGNQDSPADRIVQDIITLNDLP"
     gene            complement(1970..>2477)
                     /locus_tag="VFU20_07190"
     CDS             complement(1970..>2477)
                     /locus_tag="VFU20_07190"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5286277.1"
                     /translation="GASLSSAVGRAREFVRVALRAAPGLGGGAGPIGQGDVRLDVGAG
                     PRLNQVTVTGTDYDRSVAFYRSLGLKQIVANPPDYARFETAGGATLSVQIDPEETVSA
                     TTAIYLECDDLDERVERIARTGVPFEHGPRNQPWMWREARLRDPDGNIIFLYHAGEAR
                     RFPPWRID"
BASE COUNT          380 a          833 c          848 g          416 t
ORIGIN      
        1 atcgcctggc ttcgtgcagg caagcaggat gcgatagagc aatgcctccg gcttttgcgt
       61 cggatgcgcc ttcaggccgt cctcgtcctt cgcgcgctcc tgtccgctgc agatcggcag
      121 cacccagtca gagcgcattt gcaggtcatc gttgagcgcc ttcatcgagc ggtagttgaa
      181 cgtgtagcgc gccttctcgt ccttggcgca ccagatcaat gtctcgtggg cgttggtgaa
      241 gcgcgtgccg cggaagttgg gcatcgggtt cgacttgcgc cagacgatgt cgttcaggat
      301 ccagtagccg gcgtcctgga gcagcgctcc gacccggaag atgttgtggt agctgccgat
      361 cacccagatc gagccgtcgt ccttcaggat ccgccgggcc tcgcccagcc atcggcgggt
      421 gaagtggtcg tagtgggcga tattgtcgaa cttgtcccat tcgtcgtcga ccgcgtcgac
      481 ccggctcccc tccggccgga acaggtcgcc gccgagctgc aaattgtacg gcgggtcggc
      541 gaagatcatg tcgatcgact tgtcggggag ccgcgccatc gcggcgatac agtcgtcctc
      601 gatgatccgg tcgagcggaa gcgtctcgtc cgggcgactt gaacgcgggc gcaagcgccc
      661 ccggccctcc gcaatgggtg cgatgagatt catgcaatcc ccctgtcagc cggcaagggt
      721 gagtcagccg ggactcgcgg tcaagcgaac aagggttaac gcggctgtta acgagtatta
      781 acggacgaag cgggcaaagc ggcaaacaca agatgttgag ggccggcccg cccgaaagac
      841 tcaagcgatg gtggtgtgac tcaaaggact cgccgggatg ctttcgctca gcggcagctt
      901 cggctgggcg tgccgggcga tgatctcgcg caccggggcg aagcttcggc ggtgcagcgg
      961 ggtcactccc agcgtctcga ggccgcggta atgttcgggc gtcccgtagc cgcgattcgt
     1021 ttcccagccg tagcccggat agtcgcgcgc ccgctcggcc atcatccggt cgcgggtgac
     1081 cttggcgacg atcgacgcgg cggcgatcga ccggcatcgc gcgtcgccgg cgacgatcgc
     1141 cttcgacggc cgctgccagc gcgggcaggc gttgccgtcg accagcaccc aggcggggtc
     1201 gaccccgagc gcctcgaccg cccgggtcat cgccagcatc cgcgcccagt agatgttgag
     1261 ggtgtcgatc tcctccacgc tcgccacccc gaccccgatc accgcgcatt tgaccagccg
     1321 cgcatggatc gcctcgcgct tctcgatcgg cagattctta gaatcgtcga tcccgcgcgg
     1381 gaagcaattg cgattcagga tcacggccgc ggcgacgacc ggcccggcga gcggcgcgca
     1441 ccccgcctcg tcgaccccgg ccagaggctc cgggtaatcg agctcgatct tgaagcaggg
     1501 gcggcccatg gatcgccagc ttattgccgg ccgcggtgag ccgccagatg gccgatcaaa
     1561 aatctgtgga ctagattccg gccgccctgc agactttgcg gagcgcggca atccggggag
     1621 gaagacggtg ccctatttga tcccggcggt cctgagcgcg ctcgccttgg cggtcgcagc
     1681 tatcgggttc cgctccgccc accgattcaa gcgccctgcc ctgttcaaga ccggagcgat
     1741 cgtcgccgcc gtcgggctga tgctgctctg gcttctgccg tggtgggcga tcgcgcgcga
     1801 cgccggcgcc tgggcgatgc cgctgatggt cgcggcgctg gtggtggccg tttccgtgct
     1861 cggcgcgggc ttgagcacga tggcgcgagc cgcgacggga aaccaggact cgcccgcgga
     1921 ccggatcgtt caggacatca taaccctgaa cgatttgccc tgatcgcggt caatcgatcc
     1981 gccacggcgg gaagcggcgc gcctcgcccg cgtggtagag gaagatgatg ttgccgtcgg
     2041 ggtcgcgcag ccgcgcctcg cgccacatcc acggctggtt ccgcggccca tgctcgaagg
     2101 gaacgccggt gcgggcaatc cgctcgaccc gctcgtcgag gtcgtcgcac tcgaggtaga
     2161 tggcggtcgt cgccgagacc gtctcctcgg ggtcgatctg gaccgacagg gtcgccccgc
     2221 cggcggtctc gaaccgggcg tagtcggggg gattggcgac gatctgcttg aggccgaggc
     2281 tccggtagaa ggcgaccgac ctgtcgtaat ccgtgccggt caccgtcacc tggttgagcc
     2341 gcggcccggc gccgacgtcg agccggacat cgccctggcc gatcggcccg gcgccgccgc
     2401 cgagcccggg cgcggcgcgc agcgcgaccc ggacgaactc gcgcgcccga cccacggcgc
     2461 tcgacagcga cgcccct
//
LOCUS       DASTZO010000257         2671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_961946,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000257 DASTZO010000000
VERSION     DASTZO010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_961946"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /gene="rpsA"
                     /locus_tag="VFU20_07195"
     CDS             <1..1512
                     /gene="rpsA"
                     /locus_tag="VFU20_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU5286278.1"
                     /translation="DLKVGDEVEVYVDRVENVHGEAMLSRDRARREAAWDKLEAEFTE
                     NARVEGVIFGRVKGGFTVDLGGAVAFLPGSQVDIRPVRDVQPLMDLPQPFQILKMDRR
                     RGNIVVSRRAILEETRAEQRSGLIQSLAEGQVIEGVVKNITDYGAFVDLGGIDGLLHV
                     TDISYKRVGHPSEVINIGDTVKVQIVRINRETQRISLGMKQLESDPWDGAAAKYPVDG
                     TFRGRVTNITEYGAFVELEPGIEGLVHVSEMSWTKKNVHPGKIVSTSQEVDVKVLEVD
                     EEKRRISLGLKQAQSNPWADFAAKHPVGSTVEGEVKNATEFGLFVGLDGDVDGMVHMS
                     DIAWGVSGEEALQLHHKGETVKAQVLDVDVEKERISLGIKQLERGGVAAPGAGKAGAG
                     GVTKGETVTVSVLDVRDGGLEVQVGDGGVTGFIKRTDLGRDRDEQRPERFQVGQKLDA
                     MVIGFDRSKKPNFSVKALQIAEEKQAVAQYGSSDSGASLGDILGEALKQAKKE"
     gene            1645..1941
                     /locus_tag="VFU20_07200"
     CDS             1645..1941
                     /locus_tag="VFU20_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HEU5286279.1"
                     /translation="MIRSELVQKLCTDFPGLTQREVEGVVGAIFDSITEQLAGGGRVE
                     LRGFGAFSTRQRDARVGRNPRTGEAVDVDAKRVPYFKPGKEMRERLNLQEVTAE"
     gene            2033..2120
                     /locus_tag="VFU20_07205"
     tRNA            2033..2120
                     /locus_tag="VFU20_07205"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2066..2068,aa:Leu,seq:taa)
     gene            2223..>2671
                     /locus_tag="VFU20_07210"
     CDS             2223..>2671
                     /locus_tag="VFU20_07210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU5286280.1"
                     /translation="MLSARRDAVMLAILITAVVLLIWNGSTFFRQISLAGAELGREAR
                     TASIALTLNVALILFGWRQYVDFQHEAERRADGERRAAILASTDAHTGLLNRKGFADR
                     VQALCRQASQGGRDLAMLSIQINRFKAVSDRHGYEVGDTLLRSIAAA"
BASE COUNT          482 a          878 c          928 g          383 t
ORIGIN      
        1 gatctgaagg ttggcgacga agtcgaggtc tatgtcgacc gggtcgagaa tgtgcatggc
       61 gaagcgatgc tcagccgcga ccgcgcccgc cgcgaagccg cctgggacaa gctcgaagcc
      121 gagttcaccg aaaatgcccg cgtcgaaggc gtcatcttcg gccgcgtcaa gggcggcttc
      181 accgtcgacc tgggcggcgc cgtggccttt cttccgggca gccaggtgga catccgtcca
      241 gttcgcgacg tccagccgct gatggacctg ccgcagccgt tccaaatcct gaagatggat
      301 cgccgccgcg gcaacatcgt cgtgtcgcgc cgggcgatcc tggaagagac gcgggccgag
      361 cagcgctcgg gcctcatcca gagcctcgcc gagggccagg tgatcgaagg cgtcgtcaag
      421 aacatcaccg actatggcgc gttcgtcgat ctcggcggga tcgacggcct gctgcacgtc
      481 accgacatca gctacaagcg cgtcggccac ccgagcgagg tcatcaacat cggcgacacg
      541 gtcaaggtgc agatcgtccg catcaaccgc gagacgcagc gcatcagcct gggcatgaag
      601 cagctcgaga gcgacccgtg ggacggcgcc gcggccaaat atccggtcga cgggacgttc
      661 cgcggccggg tgaccaacat caccgaatat ggcgccttcg tcgagctgga gcccgggatc
      721 gaggggctgg tccacgtctc cgagatgagc tggaccaaga agaacgtcca cccgggcaag
      781 atcgtcagca cttcgcagga agtcgacgtc aaggtgctcg aggtcgacga ggagaagcgc
      841 cggatcagcc ttgggctcaa gcaggcgcag tcgaacccgt gggccgactt cgccgccaag
      901 cacccggtcg gctcgaccgt cgagggcgag gtcaagaacg cgaccgagtt cgggctgttc
      961 gtcggcctcg acggcgacgt cgacggcatg gtccacatgt cggacatcgc ctggggcgtc
     1021 tcgggcgagg aagcgctgca gctccaccac aagggcgaga cggtcaaggc gcaggtgctc
     1081 gacgtcgacg tcgagaagga gcgcatcagc ctgggcatca agcagctcga gcgcggcggg
     1141 gtcgcggctc cgggcgcggg caaggccggc gcgggcggag tcaccaaggg cgagacggtc
     1201 acggtctcgg tcctcgacgt gcgcgacggc gggctcgagg tccaggtcgg cgacggcgga
     1261 gtcaccggct tcatcaagcg caccgacctt ggccgcgacc gcgacgagca gcgccccgag
     1321 cgcttccagg tcggccagaa gctggacgcg atggtgatcg gcttcgaccg ctccaagaag
     1381 cccaatttct cggtcaaggc gcttcagatc gccgaggaga agcaggcggt cgcccagtac
     1441 gggtcgtcgg attcgggcgc gtcgctcggc gacatcctcg gcgaggcctt gaagcaggcg
     1501 aaaaaggagt aggcgcggcg ggaaagcgcc gtttcgggcg cgtccggacc ggaatccggt
     1561 tgcgcccaag cggctttcct gacagcctgt gattcaaccg gccgccgcga gggggcgcga
     1621 ggccaaagct caagcgggga caccatgatc cgttccgaac tggtgcagaa gctgtgcacc
     1681 gactttccgg gcctgaccca gcgcgaggtc gagggcgtcg tcggcgcgat cttcgattcg
     1741 atcaccgagc agctcgccgg cggcggccgc gtcgagttac gcggcttcgg ggccttttcg
     1801 acccgccagc gcgacgcccg cgtcggccgc aacccgcgga ccggcgaggc ggtcgacgtc
     1861 gacgccaagc gcgtgcccta cttcaagccg ggcaaggaaa tgcgcgagcg gctcaacctg
     1921 caggaagtga ccgccgaata agcggccgcc ggcggctccc ggccccgata ccccgatccc
     1981 cgaagcggcg agttgcagtt cgccgccgga agcgcatgat cggcgcggca atgcggacgt
     2041 ggcggaatgg cagacgctgg agacttaaaa tcttctgccc ggaactgggc gtgcgggttc
     2101 gagtcccgcc gtccgcacca agcctccgtt ttggaggcaa attgttaagc ctgatcgttt
     2161 aaccggcaga tgtgtcgagt ggagcagacc agcccggtgc cgagggagcc aggcccgcgc
     2221 gcttgctgag cgcgcgccgc gacgcggtca tgctcgccat cctgattacc gcggtcgtcc
     2281 tgctgatctg gaacggcagc accttcttcc ggcagatcag cctcgccggc gccgagctcg
     2341 gccgcgaagc gcggacggca agcatcgcgc tgaccctgaa tgtcgcactg atcctgttcg
     2401 gctggcggca atatgtcgat ttccagcatg aagccgagcg ccgcgccgac ggcgagcggc
     2461 gcgccgcgat cctcgcctcg accgacgcac acaccggcct gctcaaccgc aagggattcg
     2521 ccgaccgggt ccaggcgctg tgccggcaag cgtcgcaggg cggccgggac ctggcaatgc
     2581 tgtcgatcca gatcaaccgc ttcaaggcgg tcagcgaccg ccacggctat gaggtcggcg
     2641 acacgctgct gcgctcgatc gccgcggcga t
//
LOCUS       DASTZO010000258         4176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_178117,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000258 DASTZO010000000
VERSION     DASTZO010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4176
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_178117"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VFU20_07215"
     CDS             <1..257
                     /locus_tag="VFU20_07215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HEU5286281.1"
                     /translation="LDAIAARFRHARDQSAPRFSKPERRALSVLGLGEDCDRHGLRSR
                     YSKLVRRYHPDKNGGDRSHETRLGEVIDAYQLLKKARAFA"
     gene            complement(254..910)
                     /locus_tag="VFU20_07220"
     CDS             complement(254..910)
                     /locus_tag="VFU20_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011487287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU5286282.1"
                     /translation="MSLQLFAHPFSSYCQKVLIALWADGTGFEYRILDEQHPENMEEL
                     KRRWPFGLFPLLVDDGETVVETSPIIEHLQARHPGPNRWIPDGEPGRRVRFLDRFFDL
                     YVMTNMTRPVFDALRPEDQRDPYGVAQAMAQLRTAYDWLEANLGDGPWAAGEALTLAD
                     CAAAPSLFYADWVEEIGPARPKLAAYRARLLAHPTVARAVDEARPYRSFFPLGAPDRD
                     "
     gene            complement(907..1575)
                     /locus_tag="VFU20_07225"
     CDS             complement(907..1575)
                     /locus_tag="VFU20_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5286283.1"
                     /translation="MRKLTGAVFQSLDGVMQAPGGPDEDPSSDFRFGGWAFHFWDGSL
                     EEPFGKIIEAEYDLLLGKRTYDIFAAYWPYNQDIPIGARFQRINKYVLTHSDEPLGWE
                     HSHRLCGDTAETVAELKRSEGRDLLIQGSSTLYVPLLAAGLIDRLVIMIFPLVLGEGK
                     RIFDGSQAPGALQLVDHSVSDKGVVFLTYEPGGDVPTGSFASKEPSEAELERRAKLRQ
                     EEHA"
     gene            complement(1568..2065)
                     /locus_tag="VFU20_07230"
     CDS             complement(1568..2065)
                     /locus_tag="VFU20_07230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5286284.1"
                     /translation="MSDKLVTCLWFDKGEARKAAEFYASVFPDSRVGDAMNAPSDYPD
                     GQQGDELTVEFTVLGRPFVGLNGGPNFQANEAVSFMVLTEDQEETDRYWDAIVNNGGQ
                     ESACGWCKDQWGFSWQITPRVLLEANSSPDRAAARRAFEAMMTMKKIDIAKIEAAVAG
                     ETIHA"
     gene            complement(2101..2523)
                     /locus_tag="VFU20_07235"
     CDS             complement(2101..2523)
                     /locus_tag="VFU20_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HEU5286285.1"
                     /translation="MIYGGFDAIVEEGSATGGYTDGFVVPVPEARQEDYRALAAKMAK
                     VFRQHGATRVVEAISDDVPKGEVTDFYRAVKAEEGEKVVFSFIEWPDKDTRDQAWQKI
                     MADESLKPEGEMPFAGQRMFWGGFEPIFDTAKAELAHA"
     gene            complement(2542..2838)
                     /locus_tag="VFU20_07240"
                     /pseudo
     CDS             complement(2542..2838)
                     /locus_tag="VFU20_07240"
                     /inference="COORDINATES: protein motif:HMM:NF018893.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
     gene            complement(2835..3509)
                     /locus_tag="VFU20_07245"
     CDS             complement(2835..3509)
                     /locus_tag="VFU20_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU5286286.1"
                     /translation="MPVNPDASIEITAFRWVPEFAQGVVRDLRARWALEEAGLDYRVR
                     LLDQQRPPEYLLEQPFDQVPVFRDGEVHIFESGAIVQYIGEKDERLLPRDPQGRYRAI
                     QWTYAALNSVEPALINLLLIDIFFTGEEWAKLRRPGATDFARLKLKRISDWLGDKEWL
                     EGRFTIGDLLMVTVLRFIRHTRLVAEFPNLAAYVARGEARPAFQRALADQLEVFKQHE
                     PEGAAA"
     gene            3595..>4176
                     /locus_tag="VFU20_07250"
     CDS             3595..>4176
                     /locus_tag="VFU20_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5286287.1"
                     /translation="MTLDKLTNYTTEVRPSEPKRGYDDACGTAHALELIGERWALLVL
                     RELMLGPRRFSELRADLPGISANVLAQRLVELEQRGLVRKLRLPPPASVQVYEATDWG
                     LEAEPIVQALGRWAARSPRHDPTLPISGVSILLSFRTMIDRDKARGLDARIGFRFGET
                     GYVARLHDGAIDVERGDPNGCDVLFTAAPTALAA"
BASE COUNT          623 a         1425 c         1412 g          716 t
ORIGIN      
        1 cgctcgacgc gatcgcggcc cgcttccgcc atgcccgcga ccagtccgcg ccgcgcttct
       61 cgaagcccga gcggcgcgcc ttgtcggtgc tcgggctcgg cgaggactgc gaccgccacg
      121 gcttgcgcag ccgctactcg aagctggtcc ggcgctacca cccggacaag aacggcggcg
      181 accgcagcca cgagacaagg ctcggcgagg tgatcgacgc ctaccagctg ctcaagaaag
      241 cccgagcctt cgcctaatcg cggtcgggcg ctccgagcgg gaagaacgag cggtagggcc
      301 gggcctcgtc gaccgcccgg gcgacggtcg ggtgggcgag cagccgggcg cggtaggcgg
      361 cgagcttcgg gcgcgccggc ccgatctcct cgacccagtc ggcgtagaac agcgacggcg
      421 cggcggcgca gtcggcgagc gtgagcgcct cgccggccgc ccacgggccg tcgccgagat
      481 tggcctcgag ccagtcgtag gcggtgcgca gctgcgccat cgcctgcgcg accccatagg
      541 gatcgcgctg gtcctcgggg cggagcgcgt cgaacaccgg ccgggtcatg ttggtcatca
      601 catagaggtc gaaaaagcgg tcgaggaacc gcactcgccg gccgggctcg ccgtcgggga
      661 tccagcggtt ggggccgggg tgccgggcct gcagatgctc gatgatcggg ctggtctcga
      721 cgaccgtctc gccgtcgtcg accagcagcg ggaacagccc gaatggccag cgccgcttca
      781 gttcctccat gttctccgga tgctgctcgt cgaggatccg gtactcgaag ccggtgccgt
      841 cggcccacag agcgatcagc accttctggc agtaggacga gaacgggtgc gcgaaaagct
      901 gcaggctcaa gcgtgctctt cctgccggag cttcgcgcgg cgctcgagct cggcctcgct
      961 cggttccttc gaggcgaacg agccggtcgg cacgtcgccc cccggctcgt aggtcaggaa
     1021 cacgacgccc ttgtccgaga ccgagtggtc gaccagctgc agcgctccgg gcgcttgcga
     1081 cccgtcgaaa atccgcttgc cctcgccgag caccagcggg aagatcatga tgaccagccg
     1141 gtcgatcagc ccggcggcga gcagcggcac atacagcgtg ctgctgccct ggatgagcag
     1201 gtcgcggccc tcgctccgct tgagctcggc gaccgtctcg gccgtgtcgc cgcaaagcct
     1261 gtggctatgc tcccagccca gcggttcgtc cgaatgggtc agcacatatt tgttgatgcg
     1321 ctggaaccgg gcgccgatcg ggatgtcctg gttgtacggc cagtacgcgg cgaagatgtc
     1381 gtaggtccgc ttgcccagca gcaggtcgta ctcggcctcg atgatcttgc cgaagggctc
     1441 ctccaggctg ccgtcccaga agtggaacgc ccagccgccg aagcggaaat cgctgctcgg
     1501 atcctcgtcc ggcccgcccg gcgcctgcat caccccgtcg agcgactgga agacggcgcc
     1561 ggtgagctta cgcatggatc gtttccccgg cgacggccgc ctcgatcttg gcgatgtcga
     1621 tcttcttcat cgtcatcatc gcctcgaacg cccgcctggc cgccgcccgg tcggggctgc
     1681 tgttggcctc gagcagcacg cgcggcgtga tctgccagga aaagccccac tggtccttgc
     1741 accagccgca cgcgctttcc tgcccgccgt tgttgacgat ggcgtcccaa tagcggtcgg
     1801 tttcttcctg gtcctcggtc agcaccatga agctgacggc ctcgttggcc tggaagttgg
     1861 ggccgccgtt gaggccgacg aaggggcggc caaggacggt gaactcgacc gtgagttcgt
     1921 cgccctgttg gccgtccgga tagtcgctcg gcgcgttcat cgcgtcgccg acgcggctgt
     1981 cgggaaacac cgacgcgtag aactcggcgg ccttgcgcgc ctcgcctttg tcgaaccaca
     2041 ggcaggtgac cagtttgtcg gacatgtcgc gtctccccag gcttgccggc tcgttcgggc
     2101 tcaggcgtgc gccagctcgg ccttggcggt gtcgaagatc ggctcgaagc cgccccagaa
     2161 catccgctgc ccggcgaacg gcatctcgcc ttcgggcttc aggctctcgt cggccatgat
     2221 cttctgccag gcctggtcgc gcgtgtcctt gtcgggccac tcgatgaacg agaagaccac
     2281 cttctcgcct tcctcggcct tcacggcgcg gtagaaatcg gtcacctcgc ccttcgggac
     2341 gtcgtccgag atcgcctcga cgacgcgcgt cgcgccatgc tggcgaaaga ctttcgccat
     2401 cttggcggcg agggcgcgat aatcctcttg cctggcttcg ggcaccggca ccacgaaacc
     2461 gtcggtgtag ccgcccgtgg cgctgccttc ctcgacgatc gcgtcgaagc cgccgtagat
     2521 catgcgcttg ccgtcgaacg gcatgttggc gcccatctcc ttcatccgcg ggtcgctcat
     2581 cattttctgg ttcgcggagt cgcgcgcctg ccggttcgga tattcgaacc aggcgaagac
     2641 gatcttttcg tccggcttgg cgttcaccgc catccggaag tcggtgacct tgccggcggg
     2701 gacgtcgctg tcccaggcct cgacatggcg tttgacgccg aactcgcgca tgaccggcgc
     2761 ggcgtcggcg gcatgcttgc gataggcgtc cctgttggcc tcggggaccg gggccacgaa
     2821 tccttcgaaa taggtcatgc tgctgctcct tccggttcgt gttgcttgaa gacttcgagc
     2881 tggtcggcca gcgcgcgctg gaacgccggg cgcgcttcgc cgcgcgcgac ataagcggcg
     2941 aggttcggga actcggcgac cagcctggtg tggcggatga agcgcaggac ggtgaccatc
     3001 agcaggtcgc cgatcgtgaa gcggccctcg agccactcct tgtcgccgag ccagtcggag
     3061 atgcgcttga gcttgagccg ggcgaagtcc gtcgctccgg gccgccggag cttggcccat
     3121 tcctcgccgg tgaagaagat atcgatcagc agcaggttga tcagcgccgg ctcgacgctg
     3181 ttcaacgccg cataggtcca ctggatggcg cggtagcggc cctgcggatc gcgcggcagc
     3241 agccgctcgt ccttctcgcc gatatattgg acgatcgcgc cgctctcgaa gatgtggacc
     3301 tcgccgtcgc gaaacaccgg cacctggtcg aacggctgct cgagcagata ttcgggcggc
     3361 cgctgctggt cgagcaggcg gactcgatag tcgagccccg cctcctccag cgcccagcgg
     3421 gcgcgcaggt cgcgcacgac cccctgggcg aattcgggaa cccagcggaa cgcggtgatt
     3481 tcgatcgaag cgtcgggatt gacgggcatc ctgctctcct cggcggccgg cggcgatcgt
     3541 ccgccgcgac tcgattcgct tgcttattgg aactcttgat cctaaattgc aaccatgacg
     3601 ttagataagt taaccaacta cacgacggag gtgcggccaa gcgagccaaa acgcggctat
     3661 gacgacgcct gcggcactgc gcatgcgctc gagctgatcg gcgagcgctg ggcgctgctg
     3721 gtgctgcgcg agctgatgct cgggccgcgc cgcttttccg agctcagggc cgatcttccg
     3781 gggatcagcg ccaacgtgct cgcccagcgg ctggtcgagc tcgagcagcg cggattggtg
     3841 cgcaagcttc ggctgccgcc gccggcctcg gtccaggtct acgaggcgac cgactggggg
     3901 ctggaggcgg agccgatcgt ccaggcgctc ggccgctggg cggcgcgcag cccgcgccac
     3961 gacccgaccc tgccgatcag cggcgtgtcg atcctgctgt cgttccggac gatgatcgac
     4021 cgcgacaagg cgcgcggcct cgacgcccgg atcggcttcc gcttcggcga gaccggctat
     4081 gtcgcccggc tgcatgacgg cgcgatcgac gtcgagcgcg gtgacccgaa cggatgcgac
     4141 gtgctgttta cggccgcgcc gaccgcgctc gccgcg
//
LOCUS       DASTZO010000259         7783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_883536,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000259 DASTZO010000000
VERSION     DASTZO010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7783
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883536"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1239
                     /locus_tag="VFU20_07255"
     CDS             <1..1239
                     /locus_tag="VFU20_07255"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5286288.1"
                     /translation="AALAALAPASAASAPEEHQWNALAIAPLSAGARTGLRMVATQAV
                     EPIEFAPDRPTVELAMTLGAGDSLSALLTRAGATHHDAAQAAAMVAAAAPSIAPGTGV
                     SVVLGARNGARRRVERIALRAGLALRLEVARGDAGALQLIRHPIPVDTSPLRVRGRAG
                     DGLYWSLRSAGVSPRSAAEYLQALATQIDVGSEIAAGDRFDLVIASRRAATGERQLGP
                     LLYAGIDRSAAPDLQLVRWGPRGRASWVDAADIGRPAAAGMLWPVAGRITSGFGHRVH
                     PILRFTRFHRGIDFGAGWGSPIVAAADGQVASAGWAGGYGRQVRIAHAGGIGTSYSHM
                     SQIVAEPGSYVRRGQLIGYVGSSGLSTGPHLHYEVYRGGVPLNPLSVRFTGAPAVDHA
                     EVAAFKARLKALLSVGVKRG"
     gene            complement(1522..3159)
                     /locus_tag="VFU20_07260"
     CDS             complement(1522..3159)
                     /locus_tag="VFU20_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEU5286289.1"
                     /translation="MGSFSDEAGAAAASASALPPIGHVVEIAGSGSQIRLDAAALAAL
                     EPHPDPSVSMSGQVGSQVKMIVGSSWLIANVRTMRADEATDIVAHVDFLGEGDRDSAG
                     KLGNFRRGVTKFPIPGCQVHAVTSDDLRAVFAADDRAHIEIGTVYPTDDIRGALYIDA
                     MLSKHFAVLGSTGTGKSTSVALILHRISQLSPEGHIVMIDPHGEYSAAFKGCGELFNV
                     DNLQLPYWLMNFEEHCEVLLTTQGAERERDSDILAKLLLAARTKGKNLETLGKVTVDS
                     PIPYLLTDLNTILVNEMGKLDRAGDTLPFQRLKNKLDEIRADPRYSFMFSGMLVSDTM
                     GALIAKLFRLPSHGRPISIVDVSGVPSEITSVVVSVLARMVFDYAIWSRTEAQRPLLL
                     VCEEAHRYVPKDEHSSGQAVRRILERIAKEGRKYGVSLGLITQRPSDLAEGVLSQCGT
                     IIAMRLNNDRDQACVRAAMPEGARGFLDAIPALRNRECIVCGEGVAIPLRVRFDDLEP
                     EKRPASSDPSFASLWCQTGDEDGIIARTIKRWRGHGR"
     gene            complement(3265..4032)
                     /locus_tag="VFU20_07265"
     CDS             complement(3265..4032)
                     /locus_tag="VFU20_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02186 family protein"
                     /protein_id="HEU5286290.1"
                     /translation="MTTRSASLALAAASIALVAAAKPVLVPDISSRSVEIRYSFTGAQ
                     LLLFGAILYPRGRAPERPADIVVVLRGPVQPIVLREKQKLAGIWMNADSNRFRSAPSF
                     YAVASSRPIAQLVDERTAAIYELGLQNLQLSPGGGALPEKERRFEAGLLDLRRRQGLY
                     AEYPGGVEISEGVLYRATISIPSQVPVGTYTAETFLIDRGRVLAAATREIEIDKTGFE
                     RFVALAARRHAFIYGLVSVILSLGLGYAAGLLFRRRF"
     gene            complement(4029..4937)
                     /locus_tag="VFU20_07270"
     CDS             complement(4029..4937)
                     /locus_tag="VFU20_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5286291.1"
                     /translation="MDIYLPIAGQSVNALFIIALGFLVGVLSGMFGVGGGFLTTPLLM
                     FYGISPPIAVASAASQISGASVSGVMVHLRRGGVDFKMGAVMIVGGLFGSLAGAGLFR
                     MLQASGQIDLVIGSLYVLLLTGIGGLMLKDALVALGYVAEVRTDERPRHNRWVASLPL
                     RWRFYTSGLYMSPLAPFALGFVAGVLTMLLGIGGGFIMVPAMIYLLGMSARVVIGTSL
                     VMILAVSAAATFIHALTTQSVDIVLAVLLLLGGVIGAQYGALLTTRLKPDLLRLALAA
                     IILLVALRMALGLAWRPDEIYSIEYL"
     gene            complement(5007..5426)
                     /locus_tag="VFU20_07275"
     CDS             complement(5007..5426)
                     /locus_tag="VFU20_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286292.1"
                     /translation="MTPFRSIPVSLTAERRQAIGSMGGGRSAFAVFLEGGVYQRPIAW
                     GFDLDAYAQGGVVGIRSRDLFADGGFTLTRPLYGRFSAGLGMWGGVQPGLYRIDAGPR
                     LSMKVRNNMRVHLDWRQRLAGNAEPGSGPAVTLAADF"
     gene            complement(5602..6147)
                     /locus_tag="VFU20_07280"
     CDS             complement(5602..6147)
                     /locus_tag="VFU20_07280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286293.1"
                     /translation="MSASLRFLALAVVGWAGMRAATLGALPGAEAFTLGRGEAPPRAL
                     AAADVPAIVPTEFPAIAPAGAELMQPAVGGFAPGMQPQLIYAAAPVAVAPYYYPASIP
                     GGASYSPASLPAPRRGRLTQITPEPAAVFYAPIPQLDDWPLSRMASAAMPARRSSTIA
                     GGQSLPQPALKARLDRLQLSA"
     gene            complement(6144..7514)
                     /locus_tag="VFU20_07285"
     CDS             complement(6144..7514)
                     /locus_tag="VFU20_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HEU5286294.1"
                     /translation="MDAAELFTRIAAELALFAGIGFLIFAANDLAVDIIYFCRRAWRA
                     ARIYSRFPRAFASELEPPARPGLMAILVPAWDEAAVISSMLRATLRRIEHPHYRIFVG
                     YYRNDPATAAAIASVADPRIEPVRVEADGPTTKADCLNHLYDALLAYELASGESARAV
                     VLHDAEDVVHPLELSLFDKLIGRAGVIQLPVLPLVDPASNWISGHYCDEFAEAHAKEM
                     VVREAVGAAIPLAGVGCAIAREPLARLAAMHEGRPFVGGSMTEDYELGLRIGALGLKT
                     MFVRIPARPGDRSMVASRGHFPSSLHVAVRQKARWLGGIALAGWDRLGWRGGLGERWM
                     RMRDRRGPLAAVLLLSAYGAALLWSQIWLAEALGAPVQARLDPTLVILLQINAWLLAW
                     RIFMRACFTTSAYGLGQGLMSVPRMVVGNVIAVLAAGRAISIHLGGGAKRWDKTTHIF
                     PLGLAQ"
BASE COUNT         1226 a         2685 c         2815 g         1057 t
ORIGIN      
        1 gccgcgctgg ccgcgctcgc tcccgcatcc gctgcttcgg cgcccgaaga gcatcaatgg
       61 aacgcgctcg ccatcgcgcc gctgtcggcc ggcgcccgca ccggcctcag gatggtcgcg
      121 actcaggcgg tcgagccgat cgagttcgcg cccgaccggc cgacggtcga gctggcgatg
      181 acgctggggg ccggggattc gctgagcgct ctgctgaccc gcgccggcgc cacccaccac
      241 gacgcggcgc aggccgccgc gatggtcgcc gccgccgcgc cctcgatcgc gcccgggacc
      301 ggcgtttccg tcgtccttgg agcgaggaac ggggcccgcc gccgggtcga gcgcatcgcg
      361 ctgcgcgccg gtctcgcgct gcggctcgag gtcgcccgcg gcgacgccgg cgcgctgcag
      421 ctgatccggc acccgatccc ggtcgacacg agcccgctgc gggtccgcgg ccgcgccggc
      481 gacggcctct actggtcgct gcgctcggcc ggcgtgtcgc cgcggtcggc cgccgaatat
      541 ttgcaggcgc tggcgacgca gatcgacgtc ggcagcgaga tcgccgccgg cgaccgcttc
      601 gacctggtga tcgccagccg ccgcgcggcg accggcgagc ggcaattggg gccgctgctt
      661 tatgccggca tcgaccgctc ggccgcgccc gatctgcagc tggtcagatg gggcccgcgc
      721 ggccgcgcct cgtgggtcga cgccgccgac atcggccgcc cggccgcggc cgggatgctg
      781 tggccggtcg ccggccggat cacctcgggc ttcggtcacc gggtccaccc gatcctgcgt
      841 ttcacgcgat tccaccgcgg gatcgacttc ggcgccggct ggggcagccc gatcgtcgcc
      901 gccgccgacg gccaggtcgc gtccgcgggg tgggccgggg gctacggccg ccaggtgcgg
      961 atcgcccacg ccggcggaat cggcaccagc tactcgcaca tgagccagat cgtcgccgag
     1021 cccggcagct acgtgcgccg cggccagctg atcggctatg tcggctcgtc gggcctgtcg
     1081 accggcccgc acctccatta cgaggtctat cgcggcgggg tgccgctcaa tccgctcagc
     1141 gtgcggttca ccggcgcgcc ggcggtcgac cacgccgagg tcgccgcgtt caaggcgcgg
     1201 ctgaaggcgc tgctgagcgt gggggtgaag cggggttgat tgagtgagcg gccgcgttcg
     1261 accaaagccg aatttcagag ccgcctgaca tcgcgttagc ctgtgctacg aaacatccgt
     1321 tacacgcaaa gccaactgac gccgatgccc cgtcacgcga gtgaatcgcg tgatgttggt
     1381 cttgtcggct tgcgccgctg aggcggcttg aattcctccc cggaacgggg agggggacca
     1441 cgagcgcagc gacgtggtgg aggggccccc tccgtcagtc ccgctgcgcg ggcctgccac
     1501 ctccccgtcc cggggaggac cttatcggcc gtgcccgcgc cagcgcttga tggtgcgggc
     1561 gatgatgccg tcctcgtcgc cggtctggca ccacagcgaa gcgaagctcg ggtcggacga
     1621 ggccgggcgc ttctcgggct cgaggtcgtc gaaccggacc cgcaagggaa tcgcgacgcc
     1681 ttcgccgcag acgatgcact cgcggttgcg caaggccggg atcgcgtcga ggaagccgcg
     1741 cgcgccctcg ggcatggcgg cgcgcacgca cgcctggtcg cggtcgttgt tgaggcgcat
     1801 ggcgatgatc gttccgcact gcgacagcac gccttcggcc aggtcggacg ggcgctgggt
     1861 gatcaggccc aggctgacgc cgtacttgcg gccttccttg gcgatccgct cgaggatccg
     1921 gcgcaccgct tggcccgagc tgtgctcgtc cttgggcacg tagcggtggg cctcctcgca
     1981 gaccaatagc agcggccgct gcgcctcggt ccgcgaccag atcgcatagt cgaacaccat
     2041 ccgggccagc accgacacca ccaccgaggt gatctcggac gggacaccgg agacgtcgac
     2101 gatcgagatc ggccggccgt ggctcggcag ccggaacaat ttggcgatca gcgcccccat
     2161 cgtgtccgac accagcatgc ccgagaacat gaacgagtag cgcgggtcgg cgcggatctc
     2221 gtcgagcttg ttcttcagcc gctggaacgg cagcgtgtcg ccggcgcggt cgagcttgcc
     2281 catctcgtta accaggatcg tgttcaaatc ggtcagcagg taggggatcg gcgaatccac
     2341 cgtcaccttg cccagagtct cgagattctt gcccttggtg cgcgcggcga gcagcagctt
     2401 ggcgaggatg tcgctgtcgc gctcgcgctc ggcgccctgg gtggtcagga gcacttcgca
     2461 atgctcctcg aagttcatca gccagtaggg cagctggagg ttgtcgacgt tgaacagctc
     2521 gccgcagccc ttgaacgcgg ccgaatattc gccgtgcggg tcgatcatga cgatatggcc
     2581 ctcgggcgac agctgcgaaa tccggtgcag gatcagagcg accgaggtcg atttgccggt
     2641 gccggtgctg ccgagcaccg cgaaatgctt cgagagcatc gcgtcgatgt agagcgcgcc
     2701 gcggatgtcg tcggtcgggt agacggtgcc gatctcgata tgggcgcggt cgtcggccgc
     2761 gaacacggcg cgaaggtcgt cggaggtgac ggcatggacc tggcagccgg ggatcgggaa
     2821 cttggtcacg ccgcggcgga agttgccgag cttgccggcc gaatcgcggt cgccttcgcc
     2881 gaggaaatcg acgtgggcga cgatgtcggt cgcctcgtcg gcgcgcatcg tgcggacgtt
     2941 ggcgatcagc cagctcgagc cgacgatcat cttgacctgg ctgccgacct ggccggacat
     3001 cgacaccgac gggtcgggat ggggctcgag agcggccagc gccgccgcgt cgaggcggat
     3061 ttgcgagccc gagccggcga tctcgacgac atggccgatc ggcggcagcg ccgaagccga
     3121 agccgcggcg gctccggcct cgtcggagaa gctgcccatc tcgtcgagaa attgcttgag
     3181 attcggctga tcggtcattc gtctgctgcc ccaacccgat ttaacgtcgg caagggaagt
     3241 aaaggcgagc ggttaaaatt tggcttagaa gcgccgccgg aacagcagcc cggccgcata
     3301 gccgaggccg agcgacagga ttaccgacac caggccgtag atgaaggcat ggcggcgcgc
     3361 ggccagcgcg acgaagcgct cgaagcccgt cttgtcgatc tcgatctcgc gcgtcgcggc
     3421 ggcgagcacc cggccgcggt cgatcaggaa ggtctcggcg gtataggtgc cgaccgggac
     3481 ctggctgggg atcgagatgg tcgcccggta gagcacgccc tcgctgattt cgacgccgcc
     3541 gggatattcg gcgtagagcc cctggcggcg ccggaggtcg agcagcccgg cctcgaagcg
     3601 gcgctctttc tcgggcagcg ccccgccgcc gggcgacagc tgcaggttct gcagcccgag
     3661 ctcgtagatc gcggcggtgc gctcgtcgac cagctgcgcg atcggccgtg acgaggcgac
     3721 cgcgtagaag gacggcgccg agcggaagcg gttggaatcg gcgttcatcc agatcccggc
     3781 gagcttctgc ttctcgcgca gcacgatcgg ctggaccggg ccgcgaagca cgacgacgat
     3841 gtcggctggg cgctcgggcg cccggccgcg cgggtagagg atcgccccga acaggagcaa
     3901 ttgcgcgccc gtgaagctgt agcggatctc gacgctgcgc gacgagatgt cggggaccag
     3961 caccggcttg gccgcggcga ccaaagcgat ggatgccgcg gcgagcgcga gcgaggccga
     4021 gcgcgtcgtc acagatattc gatcgagtag atttcgtcgg gccgccaggc gagcccgagc
     4081 gccattcgaa gcgcgacgag caggatgatc gcggccagcg cgaggcgcag caggtccggc
     4141 ttcaggcggg tggtcagaag cgccccatat tgggcgccga tgaccccgcc cagcagcagc
     4201 agcaccgcga gcacgatgtc gaccgactgg gtggtcagcg catggatgaa ggtcgccgcg
     4261 gcgctcaccg cgaggatcat caccaggctg gtgccgatca ccacccgggc cgacatgccg
     4321 agcaggtaga tcatcgccgg gaccatgatg aagccgccgc cgatcccgag cagcatggtc
     4381 agcaccccgg ccacgaagcc gagcgcgaaa ggcgccagcg gcgacatgta gagacccgag
     4441 gtgtagaatc gccagcgcag gggcagcgag gcgacccagc ggttgtggcg cggccgctcg
     4501 tcggtccgga cctcggccac atagcccagc gcgaccagcg cgtccttgag catcagcccc
     4561 ccgatgccgg tcagcaggag cacgtagagc gagccgatca ccaggtcgat ctggccgctc
     4621 gcctggagca tccggaacag gccggcgccg gccagcgagc cgaacagccc gccgacgatc
     4681 atcaccgcgc ccatcttgaa gtcgaccccg ccgcggcgca ggtggaccat cacccccgag
     4741 acgctggcgc cgctgatctg gctcgcggcc gacgccaccg cgatcggcgg cgaaatgccg
     4801 tagaacatca gcagcggggt ggtcaggaag ccgccgccga ccccgaacat gcccgacagc
     4861 actccgacca ggaagccgag cgcgatgatg aacagcgcgt tgaccgactg gccggcgatc
     4921 gggaggtaga tgtccatcga gcctggaatc cctagccgca aagcgcgggc gaggccaagc
     4981 tgcgtccctg gaagtgggcg gcggccctag aagtcggccg cgagcgtgac cgccgggccc
     5041 gatccgggct cggcgttgcc ggccaggcgc tgacgccagt cgaggtggac gcgcatattg
     5101 ttgcggacct tcatcgacag ccgcggcccg gcgtcgatgc ggtacaggcc cggctgcacc
     5161 ccgccccaca tgcccagccc ggccgagaag cgcccgtaca gcggccgggt caaagtgaag
     5221 ccgccgtcgg cgaacaggtc gcggctgcgg atcccgacca ccccgccctg ggcataggcg
     5281 tcgagatcga agccccacgc gatcggccgc tggtagaccc cgccctcgag gaacacggcg
     5341 aaggccgacc ggccgccgcc catgctgccg atcgcctgcc gccgctcggc ggtcagcgac
     5401 accgggatcg atcggaacgg ggtcaccctg accccggccg cgacttcgcc gccgcgggcg
     5461 ccgccgaccg gcgaggaggt gcgcagcgat gccgcgagca gtggcgagat gttgtaggtg
     5521 aggcgcgcgc ccgcctggct cccgccgagc gtcccgccgg tcgccagcga ggccgggccg
     5581 ggctggccgc gaagcagcgc ctcaggcgga aagctgcagc cggtcgagcc gcgccttcaa
     5641 tgccggctgc ggcaggctct ggccgcccgc gatggtcgac gagcgccgcg ccggcatcgc
     5701 cgccgaggcc atgcgcgaca gcggccagtc gtcgagctgc gggatcgggg cgtagaagac
     5761 tgcggccggc tcgggggtga tttgggtgag ccggccgcgc cggggagcag ggagcgacgc
     5821 gggtgaatag gacgctcccc cggggatgct cgccgggtaa taataaggtg cgaccgcgac
     5881 cggggcggcg gcgtagatca gctggggctg catgcccggc gcaaacccgc cgacggccgg
     5941 ctgcatcagc tcggctccgg ccggcgcgat cgccgggaat tcggtcggga cgatcgccgg
     6001 gacgtcggcc gcggcgagcg ctcgcggcgg cgcctcgccg cggccgaggg tgaaggcttc
     6061 cgccccgggc agggcgccga gggtcgccgc gcgcattccc gcccagccga cgacggcgag
     6121 cgcgaggaag cggagcgacg cgctcattgc gcgagcccga gcgggaagat gtgggtggtc
     6181 ttgtcccagc gcttggcgcc gccgccgaga tggatcgaga tcgcccgccc ggcggcgagc
     6241 acggcgatga cgttgccgac caccatccgc ggcaccgaca tcaggccctg gccgaggccg
     6301 taggccgacg tcgtgaagca ggcgcgcatg aaaatccgcc aggcgagcag ccaggcgttg
     6361 atctgcagca ggatgacgag cgtcgggtcg agccgcgcct ggaccggcgc cccgagcgcc
     6421 tcggcgagcc agatctgcga ccacagcaag gcggcaccgt acgccgacag caggagcacc
     6481 gcggcgagcg gcccgcggcg gtcgcgcatc cgcatccagc gctcgccgag cccgccccgc
     6541 cagccgaggc ggtcccagcc ggcgagcgcg atcccgccca gccagcgggc cttctggcga
     6601 accgcgacgt gcaggctcga cgggaaatgg ccacggctgg cgaccatcga ccggtcgccc
     6661 ggccgcgccg ggattcgaac gaacatggtc ttcagcccga gcgcgccgat cctcaggccg
     6721 agctcgtaat cctcggtcat gctgccgccg acgaacggcc ggccctcatg catcgcggcg
     6781 agccgggcga gcggctcgcg ggcgatcgcg cagccgaccc cggccagcgg gatcgccgcg
     6841 ccgacggctt cgcggaccac catctccttg gcgtgggctt cggcgaactc gtcgcaataa
     6901 tggccgctga tccagttcga cgccgggtcg accagcggca ggaccggcag ctggatcacc
     6961 ccggcgcggc cgatcagctt gtcgaacagc gacagctcga gcgggtggac gacgtcctcg
     7021 gcgtcgtgga gcacgaccgc cctcgccgac tcgccgctcg ccagctcgta ggcgagcagg
     7081 gcgtcgtaga gatggttgag gcaatccgcc ttggtggtcg ggccgtcggc ctcgacccgc
     7141 accggctcga tccgcgggtc ggcgacgctg gcgatcgccg cggcggtcgc gggatcgttg
     7201 cggtaatagc cgacgaagat ccggtaatgg ggatgctcga tgcgcctgag ggtcgcgcgc
     7261 agcatcgacg agatgaccgc cgcctcgtcc caggcgggca ccaatatggc catcaggccg
     7321 ggccgtgcgg gcggctcgag ctcgctcgcg aacgcccgcg ggaaacggct gtagatccgc
     7381 gccgcccgcc acgcccgccg gcagaaatag atgatgtcga cggcaaggtc gttggccgcg
     7441 aagatcagga agccgatgcc cgcgaacagc gccagctcgg cggcgatccg agtgaagagt
     7501 tcggctgcgt ccatcggccc ggcgccgcta ttgcgacgcg gctggcccgg agaggattgg
     7561 gaacggtcgg tccattgcgc agcccccctg gcggcgaatc gaggaaccga gcaggccctt
     7621 gattgcataa cctagattaa taaacaagtt aatggagcaa ataatttcca gattggagcg
     7681 atttagctcg ctggcgccct gggccgccgg cgcgctatcg ttcgccgatg gacagccgga
     7741 cggcactttt gatcatcgcc gcgacggcct tgccggcgtc gcc
//
LOCUS       DASTZO010000260         5934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1144759,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000260 DASTZO010000000
VERSION     DASTZO010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1144759"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            66..1913
                     /locus_tag="VFU20_07290"
     CDS             66..1913
                     /locus_tag="VFU20_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HEU5286295.1"
                     /translation="MFEPQFSRREALAGLAAGAAAPLVWGAAPALAQGAAANEAEASA
                     LLDSIAENLLWQSPESATSLGIDKGGRAALRSRLSDRSGIGQQRLAQTLRADLMRANA
                     IDTSGLTHSTRTSVEVVRSSYATALEGFQLPYGDVPVGGWRNTPYVVIQNVGAYLDVP
                     RFLDSDHQIHAAADAEAYLERLASYPRQLDNELGRVIMARYKGLIPPGFLIDKALTQL
                     RQSAEGARKGGGLVESVERRTREKGIAGNWAERARAITTREVAPALERQIAELVLQRR
                     MATEEPGMWARPHGDEYYRWALKASTTTTMPPDEVHQMGLDELRALQGRMDPILRSLG
                     YTKGSVGERMQALGRDPRYKFAPGDQGRAEIMAFIEERLRIIRTKLPQMFNTLVKGNL
                     EVRRIPPEEELGAPGAYGGAGSIDGSIPGKFWINLRTTDLHTKFDLPDLTHHEAIPGH
                     VWQGEYANKLPLIRTLLSFGAYSEGWALYAQQLADEFGAYDNFPAGRLGYLQGIAFRA
                     CRLVVDTGLHAKRWSREQARRFFIDEIGSKLDEVVSEVDRYCSWPGQACGYKVGHSEI
                     NRQRERARAALGARFDIKAFNDAVVLGGNVPMDVLARNVGEYVRTARAA"
     gene            complement(1917..2861)
                     /locus_tag="VFU20_07295"
     CDS             complement(1917..2861)
                     /locus_tag="VFU20_07295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5286296.1"
                     /translation="MSPPAVQGPAAQAEITYTFVPVAECDMCGSGAGRHRVMGLRLNG
                     TQGRNPRRASGIAVSVKRCRDCGLVFADPRPVPADLGDHYGAPAEDYWPPDYFDYDPS
                     YFGREIGEVKRLIGFEPGMRALDIGAGLGKCMRSLGNAGFDAYGIEPSEPFHRKAISE
                     MGADPERLTLSTLEDAEFEPGSFDFITFGAVLEHLQQPSAALARAIEWLKPGGIIQIE
                     VPSSDHLVSKIINAYYVLSGTNYVTNISPMHAPFHLYEFTRRSFERNGAQLGYAIAHH
                     HYDVCEIMNLPRIIHPPLRWWMRRTDTGMQLTVYLRKG"
     gene            complement(2858..3763)
                     /locus_tag="VFU20_07300"
     CDS             complement(2858..3763)
                     /locus_tag="VFU20_07300"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5286297.1"
                     /translation="MDSSRIYMAGSGGMLGDAFHRVFGAANEIRCTDIDVNAPWLSYL
                     DFRDFEAYRADVLKFRPDYLFHLGAHTSLEYCEENPDDAYATNTLAVEHAVHIANELA
                     IPLLYISTAGIFDGAQETYDDWADPNPLGHYARSKFMGEKFVAANCRQHLVCRAGWMM
                     GGGPAKDKKFIQKLMKQLKDGKSELHVVDDKLGTPTYTVDFARNVELLLGKRFWGIYN
                     MVCPGVTSRLEVARYLVDALGLQDRVKITPVSSNYFAAEYFAARPASERLLTVKLDLR
                     GLNIMRDWKVCLDEYLAEQYSGYLD"
     gene            complement(3766..4872)
                     /locus_tag="VFU20_07305"
     CDS             complement(3766..4872)
                     /locus_tag="VFU20_07305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5286298.1"
                     /translation="MTARKTILVLCPFPQGIAAGQRLKYEQYFSDWRAAGYDITVSSF
                     IDRPMYDIAYEPGHTTAKLLGTLRGHVRRLRDLPRIGRFDLVYVFMWVTPFGTSLMER
                     LTRALARRLVYDIEDNALVGQDIPRADNPNPLARLLKGRGKTEYLIREADHVITSSPL
                     LNDICLRMNRAKRCTYISSSVDTDRFVPVNRYEGSDGVIIGWTGTYSTKPFLDLLRPV
                     FRKLAGRLRFKLRVIGNFDYELPGVDLEVVRWTREKEVEDLQAIDIGVYPLPLDEWVI
                     GKSGLKAIQYMAFGLPVVATDAGITAQVVRDGETGILVRTEDEWLDALERLVREPELR
                     RRLGEAGRRDVLAKYSTKVIAGEYRRVLQDATEG"
     gene            complement(4869..>5934)
                     /locus_tag="VFU20_07310"
     CDS             complement(4869..>5934)
                     /locus_tag="VFU20_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5286299.1"
                     /translation="DAGRLRAMKARLEAAGIDWTRRRYHKAPSAPATAFDIAAGIATA
                     LAIAKRHRVRLVHARSYIPALIGMAVKRLAGARLLFDMRGLWADERVDGGLWPAGGSL
                     YRVTKSIERRLLHSADHIVTLTYASVDEVRKLSAPHEWVPLSVIPTCTDLRRFRPRPS
                     DPGAPPTFGYVGSVGTWYLFDETLLAFRYFQAERPDARLLVVNRGEHQRIASRIAAAG
                     IDPACTEVVAAEHRDVPALIARMSAGAALVKPAYSKIASAPTKLAEYLACGIPVLGNT
                     GVGDVEILEERKVGVAVESLDEAVLRAGVARLVALTRDRDTPGRCRTAAEELFSLDRG
                     VAEYRRIYDSLLAGGGEERR"
BASE COUNT          970 a         2015 c         2010 g          939 t
ORIGIN      
        1 tgacggcaat tgcctgaccg tcgaagccga gcgcaccctt ccgccggcgt aagtaggagc
       61 cccccgtgtt cgaaccgcaa ttcagccgtc gcgaggctct ggcgggcctc gctgcaggcg
      121 ccgctgcgcc actcgtctgg ggagcggccc cggcgctggc ccagggcgcc gccgcgaacg
      181 aggccgaagc gagcgcgctg ctcgactcga tcgccgagaa cctcctgtgg caatcgcccg
      241 aaagcgccac ctcgctcggc atcgacaagg gcggtcgggc ggcgctgcgc tcgaggctca
      301 gcgaccgctc gggcattggc cagcagcggc tcgcgcagac cctgcgcgcc gacctgatgc
      361 gcgccaacgc gatcgacacg tcgggactaa cgcactcgac acgcacgagc gtcgaggtcg
      421 tgcgaagctc ctatgcgacc gcgctggaag gcttccagct tccctatggc gacgttccgg
      481 tcgggggctg gcgcaacacg ccctacgtcg tcatccagaa cgtcggcgcc tatctcgacg
      541 tgccgcgctt cctcgacagc gatcaccaga tccacgccgc cgccgacgcc gaggcctatc
      601 tcgagcggct cgcgtcctat cccaggcagc tcgacaacga gctcggccgg gtgatcatgg
      661 cccgctacaa ggggctgatc ccgccgggct tcctgatcga caaggcgctg acccagctgc
      721 gccagtcggc cgagggcgcg cgcaaaggcg gcgggctggt cgaatcggtc gagcggcgga
      781 ccagggaaaa ggggatcgcc ggcaactggg ccgagcgcgc acgcgcgatc acgaccaggg
      841 aggtcgctcc ggcgctcgag cgccagatcg ccgagctcgt tctccagcga cggatggcga
      901 ccgaggagcc gggcatgtgg gcgcgcccgc acggcgacga atattatcgc tgggcgctca
      961 aggcctcgac gacgacgacg atgccgcccg acgaggtcca ccagatgggg ctcgacgagc
     1021 tgcgcgcgct gcaaggccgg atggacccga tcctccgcag cctcggctac accaagggct
     1081 cggtcggcga gcggatgcag gcgcttgggc gcgatccgcg ctacaagttc gcgcccggcg
     1141 accagggccg cgcggaaatc atggcgttca tcgaagagcg cctccggatc atccgcacca
     1201 agctgccgca gatgttcaac acgttggtca aaggcaatct cgaggtgcgc cgcatacccc
     1261 cggaggaaga gcttggcgcg cccggggcct atggcggcgc gggctcgatc gacggctcaa
     1321 tcccgggcaa gttctggatc aacctgcgca cgaccgacct gcacaccaag ttcgacctgc
     1381 cggacctcac gcaccatgaa gcgatcccag ggcacgtgtg gcagggcgaa tatgccaaca
     1441 agcttccgct gatccgcacc ttgctgtcgt tcggcgccta ttccgaaggc tgggcgctgt
     1501 atgcgcagca gctcgccgac gagttcggcg cttatgacaa tttccccgcc ggccggctcg
     1561 gctacctcca ggggatcgcg ttccgcgcct gccggctggt ggtggacacc gggctccacg
     1621 ccaagcgctg gtcgcgcgaa caggcgaggc gcttttttat cgacgagatc ggatcgaagc
     1681 tcgatgaagt cgtcagcgag gtcgaccgct attgcagctg gccgggccag gcctgcggct
     1741 acaaggtcgg ccacagcgag atcaaccgcc agcgcgagcg ggccagggca gcgctcggag
     1801 cgcggttcga catcaaggcg ttcaacgacg cggtggtgct tggcggcaac gtgccgatgg
     1861 acgtgctggc gaggaatgtc ggcgaatatg tccggacggc gcgcgccgcc tgacgctcag
     1921 cccttgcgca ggtagacggt cagctgcatc ccggtgtcgg tgcggcgcat ccaccagcgc
     1981 agcggcgggt ggatgatccg cggcaggttc atgatctcgc agacgtcgta atggtggtgc
     2041 gcgatcgcat agccgagctg ggcgccgttc ctctcgaacg agcgccgcgt gaattcgtac
     2101 aaatggaacg gcgcgtgcat cgggctgatg ttggtcacgt aattggtgcc gctgaggacg
     2161 taataggcgt tgatgatttt cgacacgaga tggtcggacg acggcacctc gatctggatg
     2221 atgccgccgg gcttcagcca ttcgatcgct cgcgcaaggg ccgcggacgg ctgctgcaaa
     2281 tgctcgagca ccgcaccgaa ggtgatgaag tcgaagctgc ccggctcgaa ctcggcgtcc
     2341 tcgagcgtcg acagggtcag ccgctccggg tcggcgccca tctcgctgat cgccttgcgg
     2401 tggaacggct ccgacggctc gatcccgtag gcgtcgaagc cggcattgcc gagcgagcgc
     2461 atgcacttgc cgagcccggc gccgatgtcg agcgcgcgca tccccggctc gaacccgatc
     2521 agccgcttca cctcaccgat ctcgcggccg aaataggacg ggtcgtagtc gaaataatcg
     2581 ggcggccagt aatcctcggc cggcgctccg taatgatcgc caaggtcggc gggcaccggc
     2641 cgcgggtcgg cgaacaccag cccgcagtcg cggcaacgct tgaccgaaac cgcgattccg
     2701 ctggcgcgcc tggggttcct gccctgcgtg ccgttgagcc tgaggcccat cacccggtgc
     2761 cggcccgcgc ccgacccgca catgtcgcac tcggcgaccg gcacgaacgt gtaggtgatc
     2821 tccgcctgcg ccgccggccc ctggaccgcg ggcgggctca atcgagatac cccgaatatt
     2881 gctcggccag atattcgtcg aggcagacct tccagtcgcg catgatgtta agcccgcgaa
     2941 ggtcgagctt gaccgtcagc agccgctcgg acgccggccg ggcggcgaaa tattcggcgg
     3001 cgaaatagtt ggacgacacc ggggtgatct tcacccggtc ctggaggccg agcgcatcga
     3061 ccaggtagcg cgcgacctcg agccggctgg tcacgcccgg gcagaccatg ttgtagatgc
     3121 cccagaagcg cttgccgagc agcagctcga cgttgcgcgc gaagtcgacc gtgtaggtcg
     3181 gggtcccgag cttgtcgtcg acgacgtgga gctcgctctt gccgtccttc aactgcttca
     3241 tcagcttctg gatgaacttc ttgtccttgg ccgggccgcc gcccatcatc cagccggcgc
     3301 ggcagacgag gtgctggcgg caattggcgg cgacgaactt ctcgcccatg aacttgctgc
     3361 gcgcataatg gccgagcggg ttgggatcgg cccagtcgtc gtaggtttcc tgggcgccgt
     3421 cgaaaatgcc cgcggtgctg atgtacagga gcgggatcgc gagctcgttg gcgatgtgca
     3481 ccgcatgctc gaccgccagc gtgttggtcg cgtaggcgtc gtcgggattc tcctcgcaat
     3541 attcgaggct ggtgtgggcc ccgagatgga acagatagtc gggccggaat ttgaggacgt
     3601 cggcgcggta ggcctcgaaa tcgcggaaat cgaggtatga cagccacggc gcgttgacgt
     3661 cgatgtcggt gcatctgatc tcgttggccg cgccgaacac ccggtggaac gcgtcaccga
     3721 gcattccgcc gctgcccgcc atgtagatcc ggctggagtc cattctcacc cttccgtcgc
     3781 gtcctgaaga acccgccggt actcgccggc gatcaccttg gtcgagtatt ttgcgagcac
     3841 gtcgcggcgg cccgcctcgc cgagccggcg gcgaagctcc ggctcgcgca ccagccgctc
     3901 gagcgcgtcg agccattcgt cctcggttcg gaccaggatc ccggtctcgc cgtcgcgcac
     3961 cacctgcgcg gtgatgcccg cgtcggtcgc caccaccggc aggccgaacg ccatgtactg
     4021 gatcgccttc aacccgctct tgccgatcac ccactcgtcg agcggcagcg gatagactcc
     4081 gatgtcgatc gcctgcaggt cttcgacctc cttctcgcgg gtccagcgga cgacctcgag
     4141 gtcgaccccg ggcagctcat agtcgaaatt gccgatcacc ctgagcttga agcgcagccg
     4201 cccggcgagc ttgcggaaca ccgggcggag caggtcgagg aacggcttgg tgctgtaggt
     4261 cccggtccag ccgatgatca ccccgtcgct gccctcgtag cggttgacgg gcacgaaccg
     4321 gtcggtgtcg accgacgagg agatgtaggt gcagcgcttc gcccggttca tccgcaggca
     4381 gatgtcgttg agcagcggcg agctggtgat cacatggtcg gcctcgcgga tcagatattc
     4441 ggtcttgccg cggcccttga gcagccgcgc cagcgggttc ggattgtccg ccctcggaat
     4501 gtcctgcccg accagcgcat tgtcctcgat atcgtagacc agccggcggg caagggcgcg
     4561 ggtcagccgc tccatcagcg aggtgccgaa cggggtcacc cacatgaaca cgtagaccag
     4621 gtcgaagcgg ccgatccgcg gcaggtcgcg cagccgccgc acatggccgc gcaaggtgcc
     4681 gagcagcttt gccgtcgtgt gacccggctc gtaggcgatg tcgtacatcg ggcggtcaat
     4741 gaacgagctc accgtgatgt cgtagcccgc cgcccgccag tcggagaaat attgctcgta
     4801 cttgagccgc tgcccggccg cgatgccctg cgggaacggg cacaggacga ggatggtctt
     4861 gcgcgccgtc atcgccgctc ctcgcccccg ccggccagga gcgaatcgta gatccggcga
     4921 tactccgcga ctccccggtc cagcgaaaat agctcctcgg ccgcggttcg gcagcggccg
     4981 ggcgtgtccc tgtcccgggt cagcgccacc agccgggcga ctccggcgcg caggaccgcc
     5041 tcgtcgaggc tttcgacggc aacgcccacc ttgcgctcct cgaggatctc gacgtcaccg
     5101 acgcccgtgt tgccgagcac cgggattccg caggcgagat attcggccag cttggtcgga
     5161 gcgctggcga tcttggaata ggccggcttc accagcgccg ctccggcgct catccgggcg
     5221 atcagcgccg gcacgtcgcg atgctcggcc gcaaccacct cggtgcacgc cggatcgatc
     5281 cccgccgccg cgatccgact ggcgatccgc tgatgctcgc cgcggttgac gaccagcagc
     5341 cgcgcgtccg ggcgctcggc ctggaaatag cggaaggcga gcaaggtctc gtcgaacagg
     5401 taccaggtgc cgaccgaccc gacatagccg aaggtcggcg gcgcgccggg gtcggacggc
     5461 cgcggccgga aacgccgcag gtcggtgcag gtcgggatga cgctgagcgg gacccattcg
     5521 tggggcgcgg agagctttcg gacctcgtcg accgaggcgt aggtcagggt gacgatgtgg
     5581 tcggccgaat gcagcaaccg ccgctcgatg ctcttggtca ctcgatagag gcttccgccc
     5641 gccggccaca ggccgccgtc gacccgctcg tccgcccaca gcccgcgcat gtcgaacagc
     5701 agcctggcgc cggccaggcg cttgaccgcc attccgatca gcgccgggat gtagctgcgc
     5761 gcatggacca gccggacccg gtgccgcttg gcgatggcca aagcggtggc gattccggcg
     5821 gcgatgtcga aggcggtcgc gggcgcgctc ggcgccttgt ggtagcggcg gcgcgtccag
     5881 tcgatcccgg ccgcctcgag ccgcgccttc atcgctcgaa gccggccggc gtcg
//
LOCUS       DASTZO010000261         5003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_69046,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000261 DASTZO010000000
VERSION     DASTZO010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69046"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VFU20_07315"
     CDS             <1..1020
                     /locus_tag="VFU20_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010239364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HEU5286300.1"
                     /translation="ALEPVMSGVFEEFADGKQVTPAGEYPRIPYREALLKYGTDKPDL
                     RNPLLVHDVGERFRGSGFGLFAKIVDGGGVVRAVAAPNTAGLSRKFFDDMNEWARGEG
                     FAGLGYATRKAGEWGGPIAKNHGADGMDALAEALGLGPDDGIFFAAGAEAEAARLAGL
                     ARTRVAEQLDVIEKDSFRFCWIVDFPMFEFNEEQGKIDFSHNPFSMPQGELEALESKD
                     PLDILAWQYDIVCNGVELSSGAIRNHRPDIMYKAFAIAGYTSEEVDRDFAGMINAFKY
                     GAPPHGGSAPGIDRIVMLLAGEPNIREVVLFPMNQKAEDLMMNAPAAVSDKQLKELSI
                     RVVES"
     gene            complement(1017..1493)
                     /gene="greB"
                     /locus_tag="VFU20_07320"
     CDS             complement(1017..1493)
                     /gene="greB"
                     /locus_tag="VFU20_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HEU5286301.1"
                     /translation="MADSPRYITAEGFARLRAEYDELFGVERPKIVETVSWAASLGDR
                     SENADYLYGKKRLREIDRRLAHLARVMKAAKIVDPKSQAAGEVRFGATVELADEQDKR
                     RVLTIVGDDEADAGEGRIGWSAPLARALIGAKVGDERIVRLPNGEKSYEVVAIRYP"
     gene            complement(1506..3455)
                     /locus_tag="VFU20_07325"
     CDS             complement(1506..3455)
                     /locus_tag="VFU20_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU5286302.1"
                     /translation="MRRAAFLLPLMVIGASSPAQVPMQPSYRPAYAPVGNDVSWAIAD
                     WRRLRQSDRYTFGDYARFLIANPGWPGEAAMRRNAEKAMRPGENAATVLAFFRSEQPT
                     TGNGYARLADALLAGGRQAEALAAAREAWASHDLGASDEPAIYARYGPSFTRADHDRR
                     ADALLFAKKAGDAQRFLAMVSPHRAAAFTARVAMQLKWPDAEARYQPLMAGVTSDAGL
                     MMDRARYLRDGGFEASARQLFARPHNVTHKPADPQRFYEMMLLLAQGAASEGQWRTAF
                     DIARQVDDAFPAGTDISLKPLAIRDDYTSLTWLAGTAALDALRRPAEAVGLFQRYANG
                     GRSLQVETKGMYWAGRAALAAGKPTEAAAYFHRAATYPELFYGQLALERLGRSVTAPP
                     TAAPVVTDGQRIAFSGNWLVRATRQMVHQGSREEQALFVRALAESLQNDSERALAIEF
                     GQQIGRQDVPVWVSRVARNKGSAFYVRQAYPTLSGTPDGRLWSLTHGITRQESSFDRS
                     AVSHAGARGLMQLMPGTAREQAGKMGYGYDYGRLTRDPAYNVMLGSAYFARLLDNWGG
                     NVPLAVASYNAGSGNVRKWVRSNGDPRAGTDVLRWIEGIPFSETKGYVQRVIENSVVY
                     DSMRPANSPQSAMHVSRYLGKNRPG"
     gene            3544..4419
                     /gene="dapA"
                     /locus_tag="VFU20_07330"
     CDS             3544..4419
                     /gene="dapA"
                     /locus_tag="VFU20_07330"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029994215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEU5286303.1"
                     /translation="MFFGSIPALVTPFIGGRVAEDTFLELVEWQIAEGSNALVPCGTT
                     GEVATLTADEHRRVIELAVGQARGRVPVIAGCGSYSTAASIERMAAAKEVGADAALVV
                     VPYYNKPSQAGIAAHFWALAGASDLPIIVYNVPSRTVADISVETLAEVARLPTVVGVK
                     DATGNLGRVSAQRLACGEQFCQLSGNDDMALGFNAMGGVGCISVTANVAPGLCAEFQE
                     AMREGRWPQALELQDRLYPLHSALFTDASPGPVKYALSKVRPGFPTELRLPLTEPSEA
                     SRTAVDAALAHAGLA"
     gene            4428..4910
                     /gene="smpB"
                     /locus_tag="VFU20_07335"
     CDS             4428..4910
                     /gene="smpB"
                     /locus_tag="VFU20_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU5286304.1"
                     /translation="MAKPLPPPFDKKKVVAENRRARYDYFVEERLEAGIELTGTEVKS
                     LRGGEGSIAESYAAVEGDELWLINSHVPEYSHGNRLNHHPRRPRKLLLKGREIAKLQG
                     AVTRQGMTLVPLSIYFNGSGRAKVELAVARGKKMHDKRETEKERDWKREQGRLLRKHG
                     "
BASE COUNT          752 a         1705 c         1776 g          770 t
ORIGIN      
        1 gcgctcgagc cggtgatgtc gggcgtgttc gaggagttcg ccgacggcaa acaggtgacc
       61 ccggcgggcg agtatccgcg catcccgtac cgcgaggcgc tgctcaaata cggcaccgac
      121 aagcccgacc ttcgcaaccc gttgctggtc cacgacgtcg gcgagcgctt ccgcggctcg
      181 ggctttggcc tgttcgccaa gatcgtcgac ggcggcggcg tggtgcgcgc ggtcgcggcc
      241 ccgaacaccg ccggcctcag ccgcaagttc ttcgacgaca tgaacgagtg ggcgcgcggc
      301 gaaggcttcg ccgggctcgg ctatgcgacg cgcaaggcgg gcgaatgggg cgggccgatc
      361 gccaagaacc acggcgcgga cggcatggac gcgctcgccg aggcgctggg cctcggcccc
      421 gacgacggca tcttcttcgc cgccggtgcg gaagcggagg cggcgcggct cgccggcctc
      481 gcccgcaccc gggtcgccga gcagctcgat gtgatcgaga aggattcgtt ccgcttctgc
      541 tggatcgtcg atttcccgat gttcgagttc aacgaggagc agggcaagat cgacttctcg
      601 cacaacccct tctcgatgcc gcagggcgag ctcgaagcgc tcgagtccaa ggacccgctc
      661 gacatcctcg cctggcagta cgacatcgtc tgcaacgggg tcgagctgag ctcgggcgcg
      721 atccgcaacc accgcccgga catcatgtac aaggcgttcg caatcgccgg ctacaccagc
      781 gaggaggtcg accgggattt cgcgggcatg atcaacgcgt tcaagtacgg cgccccgccg
      841 cacggcggct cggcgcccgg aatcgaccgg atcgtcatgc tcttggccgg cgagcccaac
      901 atccgcgagg tggtgctgtt cccgatgaac cagaaggccg aggatttgat gatgaatgct
      961 ccggcggcgg tgtcggacaa gcagctcaag gagctgtcga tccgggtggt cgaaagctag
     1021 ggatagcgga tcgcgacgac ctcgtagctt ttctcgccgt tgggcagccg gacgatccgc
     1081 tcgtcgccga ccttcgcccc gatcagcgcc cgggccaggg gcgcgctcca gccgatccgg
     1141 ccttcgcccg cgtccgcctc gtcgtcaccg acgatcgtca gcacccgccg cttgtcctgc
     1201 tcgtcggcga gctcgaccgt cgccccgaag cgcacctctc cggccgcctg gctcttgggg
     1261 tcgacgattt tcgccgcctt catgacccgc gcgaggtggg cgaggcggcg gtcgatctcg
     1321 cgcagccgct tcttgccgta gaggtaatcg gcattttcgg accggtcgcc gagcgacgcc
     1381 gcccaggaca cggtctcgac gattttcggc cgctcgactc cgaacagctc gtcatattcg
     1441 gcgcgcagcc gagcgaagcc ttcggccgtg atgtaacggg gagagtctgc catcgaagcc
     1501 ggatatcagc ccgggcggtt ctttcccaaa tagcgcgaga cgtgcatcgc cgactgcggg
     1561 ctgttcgccg gccgcatcga atcatagacg acgctgttct cgatcacccg ctggacatag
     1621 cccttggtct cgctgaacgg gatcccctcg atccacctga ggacgtcggt gccggcgcgc
     1681 gggtcgccgt tgctgcgcac ccatttgcgc acattgcccg agccggcgtt gtagctggcg
     1741 accgcgagcg ggacgttgcc gccccaattg tcgagcagcc gcgcgaaata ggccgagccg
     1801 agcatcacgt tgtaggcggg gtcgcgggtc agccggccgt aatcgtaacc gtagcccatc
     1861 ttgccggcct gctcgcgcgc ggtgccgggc atcagctgca tcaacccgcg cgccccggcg
     1921 tggctgaccg cgcttcggtc gaacgagctt tcctggcggg tgatcccgtg ggtcagcgac
     1981 cacagccggc cgtcgggcgt gccggacagg gtcgggtagg cctggcggac gtagaaagcc
     2041 gagcccttgt tgcgcgccac tcgcgacacc cacaccggca cgtcctggcg gccgatctgc
     2101 tggccgaatt cgatcgccag cgcccgctcg gaatcgttct ggagcgattc ggcgagcgcc
     2161 cggacgaaca gcgcctgctc ctcgcggctg ccctggtgca ccatctgccg cgtcgcgcgc
     2221 accagccagt tgccgctgaa cgcgatgcgc tggccgtcgg tgaccaccgg ggcggccgtg
     2281 ggcggcgcgg tcaccgaccg gccgagccgc tcgagcgcca gctggccgta gaacagctcg
     2341 gggtaggtcg cggcgcggtg gaagtaagcc gccgcctcgg tcggcttgcc ggcggccaga
     2401 gcggcgcggc ccgcccagta catgcccttg gtctcgacct gcagcgagcg gcctccattc
     2461 gcgtagcgct ggaacagccc gaccgcttcg gccggccgcc gcagcgcgtc gagcgccgcg
     2521 gtcccggcga gccaggtcag cgacgtgtaa tcgtcgcgaa tggcgagcgg cttcaggctg
     2581 atgtcggtgc cggccgggaa ggcgtcgtcg acctggcggg cgatgtcgaa ggcggtccgc
     2641 cactggcctt cggacgcggc gccctgcgcg agcagcagca tcatctcgta gaagcgctgc
     2701 gggtcggcgg gcttgtgggt gacattgtgc gggcgggcga acagctgccg ggcggacgct
     2761 tcgaagccgc cgtcgcgcag gtagcgggcg cggtccatca tcagcccggc gtcgcttgtg
     2821 acgcccgcca tcagcggctg gtagcgggcc tcggcgtcgg gccatttgag ctgcatcgcg
     2881 acgcgggccg tgaacgccgc ggcgcggtgc gggctgacca tcgccaggaa gcgctgcgcg
     2941 tcgccggcct tcttggcgaa cagcagggcg tcggcgcggc ggtcgtggtc cgccctcgtg
     3001 aagctgggcc cgtagcgcgc gtagatcgcc ggctcgtcgc tcgcgccgag gtcgtgcgac
     3061 gcccaggctt cgcgggcggc ggcgagggcc tcggcctggc ggccgccggc gagcaacgcg
     3121 tcggcgagcc gcgcatagcc gttgccggtg gtcggctgct cgctgcggaa gaaggccagc
     3181 acggtcgctg cattctcccc gggccgcatc gctttctcgg cgttgcggcg catcgccgcc
     3241 tcgccgggcc agccgggatt ggcgatcagg aagcgcgcat agtcgccgaa ggtatagcgg
     3301 tcgctttgcc ggagccggcg ccagtcggcg atcgcccacg aaacgtcgtt gccgacgggg
     3361 gcgtaggcgg gccggtagct cggctgcatc ggcacctgcg ccgggctcga cgcgccgatg
     3421 accatcaacg gcaggagaaa tgcggctcgc ctcatgctgg acatcctgcg gtcgcggtcc
     3481 ttatcagtcc ctgaacataa cagaccccac ggcaatcgga atcctcctgg aggacttaag
     3541 ttcatgtttt tcgggtctat cccggcgctg gtgacgccgt ttattggcgg gcgtgtcgcc
     3601 gaagacacat tccttgagct ggtcgaatgg caaatcgccg agggcagcaa cgccctggtg
     3661 ccgtgcggga cgaccggcga agtcgcgact ttgaccgccg acgagcaccg gcgggtgatc
     3721 gagctggcgg tcgggcaggc gcggggccga gtgccggtga tcgccggctg cggaagctat
     3781 tcgaccgccg cgtcgatcga gcggatggcg gcggccaaag aggtcggcgc cgacgccgcg
     3841 ctggtcgtcg tgccctatta caacaagccc agccaggccg gcatcgccgc ccatttctgg
     3901 gctctggccg gggcctcgga cttgccgatc atcgtctaca acgtgccctc gcggaccgtc
     3961 gccgacatct cggtcgagac cttggccgag gtcgcccggc tgccgaccgt cgtcggggtc
     4021 aaggacgcga ccggcaacct cggccgggtc tccgcccagc ggctggcctg cggcgagcag
     4081 ttctgccagc tcagcggcaa cgacgacatg gccctgggct tcaacgcgat gggcggggtc
     4141 ggctgcatct cggtcaccgc caacgtcgcg cccgggttgt gcgccgagtt ccaggaggcg
     4201 atgcgcgagg gccgctggcc gcaggcgctc gagctccagg accggctcta cccgctgcac
     4261 tcggccctgt tcaccgacgc ctcgcccggc ccggtcaaat atgcgctgtc gaaggtccgc
     4321 ccgggcttcc cgaccgagct gcggctgccg ctgaccgagc cgtcggaggc gtcgcggacc
     4381 gcggtcgacg ccgcgctcgc ccacgccggg ctcgcctgag cgggccgatg gccaagccgc
     4441 tgccgccgcc gttcgacaag aagaaggtcg tcgccgagaa ccggcgcgcg cgctacgact
     4501 atttcgtcga ggagcgcctg gaggccggga tcgagctcac cggcaccgag gtcaagtcgc
     4561 tgcgcggcgg cgagggctcg atcgccgaaa gctacgccgc ggtcgaaggc gacgagctgt
     4621 ggctgatcaa cagccatgtc cccgagtaca gccacggcaa ccggctcaac caccacccgc
     4681 ggcggccgcg gaaactgttg ctcaagggcc gcgagatcgc caagctgcag ggcgcggtga
     4741 cgcgccaggg gatgacgctc gttccgctgt cgatctattt caacggcagc ggccgggcga
     4801 aggtcgagct ggcggtcgcc cgcggcaaga agatgcacga caagcgcgag accgagaaag
     4861 agcgcgactg gaagcgcgag cagggccggc tgctgcgaaa gcatggctga cccactggaa
     4921 attgacggga gcgccgcggc gccccgggtt tgcgtggagg gatgaatatg gttcgagtga
     4981 ttgtgttgct ggcgacgacg gcg
//
LOCUS       DASTZO010000262         4423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1249188,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000262 DASTZO010000000
VERSION     DASTZO010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249188"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1525
                     /locus_tag="VFU20_07340"
     CDS             <1..1525
                     /locus_tag="VFU20_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HEU5286305.1"
                     /translation="KFVTGKALGLGLRPIVVVNKIDRPDARPAEVLDEVFELFLNLDA
                     SDEQLDFPTLYASGRAGYAGLEDSIRPENTPGADLTPLFETIVAHVPPPTVVADGPFA
                     MLATLLDRDNFVGRVLTGRIASGRLAINMPIRALDPDGKVVEEGRATKIMAFRGLERV
                     PVESAEAGEIVAIAGLTAATVANTIADPSVTEPLHALPIDPRTLAMTFSVNDSPMAGR
                     EGTKVTSRMIRDRLAREAEGNVSFHITESADKDSFEVAGRGELQLGVLIETMRREGFE
                     LSISRPRVLFRDGAESATGGREEPYETVVVDVDDAYASTVVSKIQLRKGELVEQRPSS
                     GGKTRLVFSAPSRGLIGYHGEFLSDTRGTGIMNRLFERYGPYKGPIEGRQTGVLISNA
                     SGQAVAYALNALEDRGALMVSPGEPVYEGMIVGENAKPEDLEVNPLKSKQLTNFRASG
                     PKEDAIRLTPPKRLTLEQAIAYIDEDELVEVTPKAIRLRKRHLDPHERKRANRAKAA"
     gene            complement(1555..2409)
                     /gene="ygiD"
                     /locus_tag="VFU20_07345"
     CDS             complement(1555..2409)
                     /gene="ygiD"
                     /locus_tag="VFU20_07345"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006833878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HEU5286306.1"
                     /translation="MARMPALFVGHGSPMNTLERNGFTGAWREFGKQLPRPRAVLVVS
                     AHWFFGATAVTAMPWPRTIHDFYGFPSELFDFQYPAPGAPDVAEEVAEAVKPVWCGLD
                     RDQWGLDHGTWSVLAHLYPQADVPVVQLSINALKPLEYHLDLGTRLASLSERGILFIA
                     SGNVVHNLSRIQWDRPDAAFDWAERFDDAVARQLSEAPAEILRLAEHPDFHAAVPTPD
                     HFIPLLYVAGMAARHGRPLDALVRGYAMGSISMSCYGLEADIKLAGQEAEGAAALPEG
                     VPADQSNI"
     gene            complement(2514..2933)
                     /locus_tag="VFU20_07350"
     CDS             complement(2514..2933)
                     /locus_tag="VFU20_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5286307.1"
                     /translation="MTTIALRAPGSNWAASDLAATGGRLLLAAIFLLSGYSKLADPAA
                     TIGYIQSVGLPLPQVGVALAIAAELVGGALLVIGYRTRLAAAALAVFTLVTALFFHSA
                     LGDQNQFIQFFKNVAITGGLLQVIAFGPGRLSLDGRA"
     gene            3062..3979
                     /locus_tag="VFU20_07355"
     CDS             3062..3979
                     /locus_tag="VFU20_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013849507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5286308.1"
                     /translation="MPAVGTPSLDQLQVFLAIVDEGSFAAAARKLNRAVSAVSYAISN
                     LEAQLGLGLFHRQGSRKPVLTDAGQALLADARLVAADLQALRARALGLHSGLEPEVSI
                     ALDVMIPLDRVAGVLREFQEIYPTVNLRLHVEALGGVAALLLDGRAQLGISGPAVTEH
                     PDLERAEAGALLLVPVASPGHPLARSGKLAPGAARAHLQLVLTDRSNLTEGRDFTVAS
                     PRTWRLADLGAKLELLREGIGWGNMPLGMIEDDLAHGRLVRLKMPEAIGGPYRLAGLW
                     RRDDPPGPATRWLFDALVGVLSTEKKQFV"
     gene            complement(4076..>4423)
                     /locus_tag="VFU20_07360"
     CDS             complement(4076..>4423)
                     /locus_tag="VFU20_07360"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5286309.1"
                     /translation="KTGKWGLILKTLDDTDMGSDRLTIQPGGFSGWHAHPGPVFVTVT
                     QGSVIWYDGSNPLCTPHTFATGESFIEGAYRVHNVQNASNSTVAEFVAITIKPVGFVG
                     PAFRLDRAEPTNC"
BASE COUNT          732 a         1511 c         1507 g          673 t
ORIGIN      
        1 caagttcgtg accggcaagg cgctcggcct tggcctgagg ccgatcgtcg tcgtcaacaa
       61 gatcgaccgg ccggacgcgc gaccggccga ggtgctcgac gaggtgttcg agctattcct
      121 caacctcgac gcgagcgacg agcagctcga tttcccgacg ctctacgcga gcggccgcgc
      181 cggctatgcg gggctcgagg acagcatccg cccggaaaac acacctggcg ccgacctgac
      241 gcccctgttc gagaccatcg tcgcccacgt gcctccgccg accgtggtcg ccgacgggcc
      301 gttcgcgatg ctcgccacct tgctcgaccg cgacaatttc gtcggccggg tgctgaccgg
      361 gcggatcgcc agcgggcgcc tcgcgatcaa catgccgatc cgggcgctcg accccgacgg
      421 caaggtcgtg gaagagggcc gggcgaccaa gatcatggcc tttcgcggac tcgagcgggt
      481 gcccgtcgag agcgcggaag ccggcgagat cgtcgccatc gccgggctca ccgcggcgac
      541 cgtggccaac accatcgccg acccgtcggt gaccgagccg ctgcacgctt tgccgatcga
      601 cccgcggacc ctggcgatga ccttcagcgt caacgattcg ccgatggccg ggcgcgaggg
      661 gaccaaggtc accagccgga tgatccgcga ccgcctggct cgcgaggccg agggcaacgt
      721 ctccttccac atcaccgaaa gcgccgacaa ggacagcttc gaggtcgccg gccgcggcga
      781 actccagctc ggcgtcctga tcgagaccat gcgccgcgag ggcttcgagc tgtcgatcag
      841 ccgcccgcgg gtgctgttcc gcgacggcgc cgaatccgcg accggcggcc gcgaggagcc
      901 gtacgagacc gtcgtcgtcg acgtcgacga cgcctacgcc tcgaccgtgg tgtccaagat
      961 ccagctgcgc aagggcgagc tggtcgagca gcgcccgtcg agcggcggca agacgcgcct
     1021 cgtgttctcg gccccgagcc gcggcctgat cggctatcac ggcgagttcc tgtccgacac
     1081 ccgcggcacc ggtatcatga accggctgtt cgagcgctac ggcccgtaca aggggccgat
     1141 cgagggccgc cagacgggcg tcctgatctc caacgccagc ggccaggcgg tcgcctatgc
     1201 gctgaacgcg ctcgaggacc gcggcgcgct gatggtctcg cccggcgagc cggtctacga
     1261 gggcatgatc gtcggcgaga acgccaagcc cgaggacctc gaggtcaacc cgctgaaatc
     1321 caagcagctg accaacttcc gcgcctcggg ccccaaggaa gacgcgatcc ggctgacccc
     1381 gcccaagcgc ctgaccctcg agcaggcgat cgcctacatc gacgaggacg agctggtcga
     1441 ggtgacgccc aaggcgatcc ggctgcgcaa gcgccacctc gacccgcacg agcgcaagcg
     1501 cgccaaccgg gccaaggccg cctagcttcc cggaacgccc gccgtgggcg tcgttcagat
     1561 attgctctgg tcggccggga cgccctcggg cagggccgcg gcgccttccg cctcttgccc
     1621 ggcgagcttg atgtcggcct cgagaccgta acagctcatc gagatcgacc ccatcgcata
     1681 tccgcgaacg agcgcgtcga gcggccggcc gtggcgggcg gccatgccgg cgacgtacag
     1741 caaggggatg aaatgatcgg gggtcggcac ggcggcgtgg aaatcggggt gctcggccag
     1801 ccggagaatc tcggcgggag cctcggacag ctgccgggcc acggcatcat cgaaccgctc
     1861 cgcccagtcg aacgcggcgt cgggccgatc ccactggatg cggctcaggt tgtggacgac
     1921 gttgccgctt gcaatgaaca gaattccacg ctcgctcagc gacgcgaggc gagtcccgag
     1981 atccaggtga tattcgagcg gcttcagcgc attgatcgac agctggacca ccggcacgtc
     2041 cgcttgcggg tagaggtggg caagaacgct ccaggtgccg tggtcgagtc cccactggtc
     2101 gcggtcgagc ccgcaccaga ccggcttgac ggcctcagca acctcctcgg cgacgtccgg
     2161 agcgccgggc gccggatact ggaagtcgaa cagctcgctc ggaaagccat agaagtcgtg
     2221 gatcgtgcgc ggccacggca tggcggtgac ggcggtggct ccgaagaacc aatgcgcgga
     2281 cacgaccagc acggccctcg gcctgggcag ctgtttgccg aattctcgcc acgccccggt
     2341 aaagccgttg cgctcgaggg tgttcatcgg gctgccgtgg ccgacgaaca atgcgggcat
     2401 gcgcgccatt gcgttcctcc ctgtgcgctg ccgcccacgg gctagaacca tgggcggcgg
     2461 cttgcgatcc cggtcgggcg gtgagcccga aggctgcgcg accaaagccc tgcttatgct
     2521 cggccgtcga ggctgagccg ccccgggccg aaggcgatca cctgcagcag cccgccggtg
     2581 atggcgacgt tcttgaagaa ctggatgaac tggttctggt cgccgagcgc cgagtgaaag
     2641 aacagggcgg tgaccagggt gaagacggcc agcgcggcgg cggcgagccg ggttcggtag
     2701 ccgatgacga gaagggcgcc cccgaccagc tcggcggcga tggccagggc cactccgact
     2761 tggggcagcg gcaggccgac ggactggatg tagccgatgg tcgccgccgg gtcggcgagc
     2821 ttcgaatagc cgctgagcag gaagatggcg gccagcagca ggcggccgcc ggtcgccgcg
     2881 aggtcggacg ccgcccaatt cgagccgggc gcgcgcaatg cgatggtggt catggttcgg
     2941 tctcctgttg ccagccggtg ctggttgcag aagccagata ggcggtttcg ttgttcggtt
     3001 ttagccgaca aatactgatt ataatgttca ggtttctaga acatcatgct acggtcgccg
     3061 catgcccgcc gtcggaacgc ccagcctcga tcagctccag gtgttcctcg caattgtcga
     3121 cgaaggcagc ttcgccgccg ccgcccgcaa gctgaaccgc gcggtgtcgg cggtcagcta
     3181 cgcgatctcc aatctggagg cgcagctcgg cctcggcctg ttccatcgcc agggctcgcg
     3241 caagcccgtg ctgacggatg cgggccaggc gctgttggcg gacgcccgct tggtcgccgc
     3301 cgaccttcag gccctccgcg cccgcgccct cggcctgcat agcggcctgg agccggaagt
     3361 atccatcgcg ctggacgtga tgatcccgct ggaccgcgtc gcgggcgtgc tgcgcgaatt
     3421 ccaggagatc tatccgacgg tcaatctgcg cctgcacgtg gaggccttgg gcggggtcgc
     3481 ggccctattg ttggacggcc gggcgcagct cggcatcagc ggtccggccg tcaccgagca
     3541 tcccgatctt gaacgagccg aggcgggggc gttgctgctg gttccggtcg cttcgcccgg
     3601 ccaccctctc gcgcgctccg gaaagctcgc ccccggagcc gcccgagcgc acctccagct
     3661 tgtcctgacc gaccgctcca atctgaccga agggcgggat ttcacggtcg ccagtccgcg
     3721 cacctggcgg ctggctgacc tcggggccaa gctcgaactg ctccgcgaag gcatcggctg
     3781 gggcaacatg ccgctcggca tgatcgagga cgacctcgcg cacgggcgat tggtccggct
     3841 gaagatgccc gaggccatcg gcggccctta ccgcctggcg gggctctggc gccgcgacga
     3901 tccgcccggg ccggcgaccc ggtggctgtt cgacgccctc gtcggcgtgc tttcgacgga
     3961 aaaaaagcag ttcgtctaat attctgcgca agcgccacct cgacccgcac aagcgcaagc
     4021 ggcaggcacg ccgggcggag gcaacctgac gccgcctcgg cgcaccccgc cgcaattagc
     4081 agttggtggg ttccgcccgg tcgagccgga atgcgggccc aacgaacccc actggcttga
     4141 tcgtgatcgc gacgaattcc gctacggttg aattcgaggc gttttgaaca ttgtggactc
     4201 gataggcgcc ttcaatgaaa gactcgccgg tcgcgaacgt gtgtggagtg cagagcgggt
     4261 tcgacccatc gtaccagatc acgctgccct gggtgacggt tacgaacact ggccccggat
     4321 gagcgtgcca gccgctgaag ccaccgggtt gtatggtgag ccggtcggag cccatgtccg
     4381 tgtcgtccaa ggtcttcaag atcaggcccc atttgcccgt ctt
//
LOCUS       DASTZO010000263         7626 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_99320,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000263 DASTZO010000000
VERSION     DASTZO010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99320"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VFU20_07365"
     CDS             complement(<1..938)
                     /locus_tag="VFU20_07365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU5286310.1"
                     /translation="MSTIPADALPLKAHEAPDVHDADHKPGFFTRWFMSTNHKDIGTL
                     YLIFAIVAGIIGGAISGIMRAELAEPGIQILSQGWPLGPGTANFDEWAHHWNVLITAH
                     GLIMVFFMVMPAMIGGFGNWFVPLMIGAPDMAFPRMNNISFWLLIPAFCLLLGSSFVS
                     GGSGLGAGTGWTVYAPLSTAGSAGPAVDMAIFSLHLAGASSILGAINFITTIFNMRAP
                     GMTLHKMPLFVWSILVTAFLLLLALPVLAGAITMLLTDRNFGTTFFDASGGGNPVLYQ
                     HLFWFFGHPEVYILILPGFGIISHIVSTFSRKPVFG"
     gene            complement(954..1898)
                     /gene="coxB"
                     /locus_tag="VFU20_07370"
     CDS             complement(954..1898)
                     /gene="coxB"
                     /locus_tag="VFU20_07370"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEU5286311.1"
                     /translation="MPAAQVTPPTPGLGVPVGGKGIQEQVTGIGREAAGFHNGPLLIL
                     CAAISLFVLGLLGYAMVRYRRSAHPKPSRTSHNTTIEVIWTLVPVLILVAIAIPSIRL
                     LRHQYSPPKADLTVKVIGNQWYWTYQYPDNGDIEIVSNMLKESNDPTRQPKERARTDA
                     DGPPLLAVDERLVIPAGKVVKFIITSNDVIHAFWIPAFWNKIDANPGQLNEIWAKVDR
                     PGVYFGQCTELCGARHAYMPIAVEAVAPARFAAWVASKGGSMPGARPAPTPDSTGASP
                     VSEAAVPAAAQPQPPTPSAADPAAVRQPATNQAATAQN"
     gene            2231..2815
                     /gene="pyrE"
                     /locus_tag="VFU20_07375"
     CDS             2231..2815
                     /gene="pyrE"
                     /locus_tag="VFU20_07375"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU5286312.1"
                     /translation="MTDDEILDEFRAAEALLEGHFILSSGLRSPRYLQCARVLMDPAR
                     AGRLASALAAKLPAELRDGIDAVVSPAMGGVIIGHEMGRALGRRAMFVERPQGPFELR
                     RGFRLEPGERVLLVEDVVTTGLSSREAIAAITEAGGRVVGAAALVDRSGGTADLGVPL
                     TALIRIDVPTYAADELPPELASIPAVKPGSRAAA"
     gene            2812..3540
                     /locus_tag="VFU20_07380"
     CDS             2812..3540
                     /locus_tag="VFU20_07380"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEU5286313.1"
                     /translation="MSSPLRLGVNIDHVATIRNARGGAHPDPVRAAIAAAAAGADGIT
                     AHLREDRRHITDEDIDRLMGQIDLPLNLEMAATEEMLAIALAHRPHAACIVPEKREER
                     TTEGGLDAAGQHNHLAYYVDRLGSANIRVSLFIEPREEQVDAALRLGAPVVEFHTGRY
                     AHVDGEERATELKRIADCAALAVKAGIEPHAGHGLTFDNVAPVAAIPQLAELNIGHFL
                     VGEAIFTGLEASVRQMRELMDAAR"
     gene            3650..4057
                     /gene="acpS"
                     /locus_tag="VFU20_07385"
     CDS             3650..4057
                     /gene="acpS"
                     /locus_tag="VFU20_07385"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017672501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU5286314.1"
                     /translation="MIVGIGSDLCNIERIERSLERFGDRFLERVFTATERAKADSRPF
                     TRAGTLAKRFAAKEAFSKAVGTGFKHGVFMKDIGVVNAPSGAPTLELTGGARATLDAL
                     APAGHAIDIHLTLTDEYPWAQAFVILTARKMEP"
     gene            4137..4931
                     /gene="lepB"
                     /locus_tag="VFU20_07390"
     CDS             4137..4931
                     /gene="lepB"
                     /locus_tag="VFU20_07390"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5286315.1"
                     /translation="MWREIKGLLWVLVAVLAFHSFVAKPFYIPSESMMPGLLKGDRLV
                     VSKYPYGWSWVSPSFHVFPHWEGRLLGAMPKRGDIVIVTPPGQSDDYIKRVIGLPGDT
                     VEVRRGRLILNGRPVRSEVRPSALIPVDPNVPCGIEFSGFVVRSGGREYCRLPIVRET
                     LPGGPSYDTVDLGESPGDDFGPVKVPADHVFMMGDNRDRSADSRFGLGPPENGLGGPV
                     PWENIGGRAEFITFSLDGTTRIYDPVSWFRALRSGRAGRSLRGSAG"
     gene            4932..6032
                     /locus_tag="VFU20_07395"
     CDS             4932..6032
                     /locus_tag="VFU20_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5286316.1"
                     /translation="MAEPPTVEPHVERPGPAEFRDPLVRRELARAMVWFGAALLIAGT
                     IVLAQPLLLIVGGMIFAVILDGGTRLLGRYLPIARGWRLALVVFLGLGFVAWVFYYAG
                     TTFAAQAENLRAVVEVQAARAYAWAQDFGLVSDPTPGSIGQQLMGSVGRLTTAVGSAL
                     GAVTSAILIFVIGIFIAIDPRLYDRGVAWMLPLRYRDRFYAIGDRVGWTLRRLLAGRL
                     VGMLFEGVFTWLMLGYVPQLFGLAPVPMAALLGLITGLLAFVPNIGAIISGVLMVAVG
                     FSAGVDQGLWSIFVYFLVQNVDGYLVIPYIAKRTVDLAPAVVLAAQLLFGALFGFLGL
                     LLADSILAALKVTLQELSKYRADDEGELPEVA"
     gene            complement(6029..6559)
                     /locus_tag="VFU20_07400"
     CDS             complement(6029..6559)
                     /locus_tag="VFU20_07400"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEU5286317.1"
                     /translation="MSASTLAHLERRRGTGKLWLAILLLIAAGVGLAWLGAGSLRGET
                     TASGLGFRTVEAGEGPLIKAVDGVLIEYEGRLEDGTVFDTSEGRGPTPMIAGQVIPGF
                     AEALTRMQKGGRYRIQIPSALGYGANPQPGSPIPPNADLEFDVHVVQVVPNAALMQGI
                     QQGPEQQPPEQPAPQP"
     gene            complement(6597..6806)
                     /gene="rpsU"
                     /locus_tag="VFU20_07405"
     CDS             complement(6597..6806)
                     /gene="rpsU"
                     /locus_tag="VFU20_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEU5286318.1"
                     /translation="MQIIVRDNNVEQALRALKKKLQREGVYREMKLRRHYEKPSEKRA
                     RERAAAIRRARKLERKRAEREGTAR"
     gene            7124..>7626
                     /locus_tag="VFU20_07410"
     CDS             7124..>7626
                     /locus_tag="VFU20_07410"
                     /inference="COORDINATES: protein motif:HMM:NF025029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HEU5286319.1"
                     /translation="MLRLGAVGVGGAMLSSAASSVGLPSVVFQAPRSPLPPVPAAPRA
                     PYGVDQRIFERARAALDAHRIYHRDFIGIADFSLPSSDPRFHVVDLRNGQVESYRVAH
                     GQGSDPNHNGFLDRFSNEFGSYATSNGTYTTGEYYHGKYGLSMKTHGHDWSNSNAEPR
                     AIVIHNA"
BASE COUNT         1126 a         2611 c         2646 g         1243 t
ORIGIN      
        1 tagccgaaga cgggcttgcg gctgaaggtc gagacgatgt gcgagatgat gccgaacccg
       61 ggcaggatca ggatgtacac ctcggggtga ccgaagaacc agaacaggtg ctggtagagg
      121 accgggttgc cgccgcccga cgcgtcgaag aaggtggtgc cgaagttgcg gtcggtcagc
      181 agcatcgtga tcgcgccggc gagcaccggc agcgcgagca gcagcaggaa ggcggtgacc
      241 aggatcgacc agacgaacag cggcatcttg tgcagggtca tgcccggagc gcgcatgttg
      301 aagatggtgg tgatgaagtt gatcgccccg aggatcgaac tggcgccggc gaggtggagc
      361 gagaagatcg ccatgtcgac cgcgggcccg gccgagccgg cggtcgacag aggcgcatag
      421 accgtccagc cggtcccggc cccgagcccg gacccgccgg agacgaagct cgagcccaaa
      481 agcaggcaga aggccgggat cagcagccag aagctgatgt tgttcatccg cgggaacgcc
      541 atgtcgggcg cgccgatcat cagcggcacg aaccagttgc cgaagccgcc gatcatcgcc
      601 ggcatgacca tgaagaagac catgatcagc ccgtgcgcgg tgatcagcac gttccagtgg
      661 tgcgcccatt cgtcgaagtt cgcggtcccc ggcccgagcg gccagccctg gctcaaaatc
      721 tggatgccgg gctcggcgag ctcggcgcgc atgatcccgg aaatcgcgcc gccgatgatc
      781 cccgcgacga tcgcgaagat cagatagagc gtgccgatgt ccttgtggtt ggtggacatg
      841 aaccagcggg tgaagaagcc cggcttgtgg tccgcgtcgt ggacgtcggg cgcctcatgg
      901 gccttcagcg gcagggcgtc ggcggggatg gtgctcatga tactcgtttc cgcttaattc
      961 tgggccgtcg cggcctggtt cgtggccggc tgccggaccg ccgccggatc ggcggccgag
     1021 ggtgtcggag gctggggctg cgccgccgcc gggaccgcgg cctcgctgac cggcgacgcg
     1081 ccggtgctgt cgggagtcgg ggcgggcctg gcgccgggca tcgagccgcc cttcgaggcc
     1141 acccaggcgg cgaaacgcgc cggagccacc gcctcgaccg cgatcggcat gtaggcgtgg
     1201 cgggcgccgc acagctcggt gcactggccg aaatagactc ccggccggtc gaccttggcc
     1261 caaatctcgt tcagctggcc ggggttggcg tcgatcttgt tccagaaggc cgggatccag
     1321 aaggcgtgga tcacgtcgtt cgaggtgatg atgaacttca ccaccttgcc ggccgggatc
     1381 accagccgct cgtcgaccgc cagcagcggc ggcccgtcgg cgtcggtccg cgcccgttcc
     1441 ttgggctggc gtgtggggtc gttcgactcc ttgagcatgt tggagacgat ctcgatgtcg
     1501 ccgttgtccg ggtattggta ggtccaatac cattggttgc cgatcacctt gaccgtgagg
     1561 tcggccttgg gcggcgaata ttggtggcgc aggaggcgga tcgagggaat tgcgatggcg
     1621 accaggatca gcaccgggac gagggtccag atgacctcga tcgtcgtgtt gtgcgaggtg
     1681 cgcgacggct tcggatgggc gctgcggcgg tagcggacca tcgcgtagcc gagcagcccg
     1741 agcacgaaca ggctgatcgc ggcgcacagg atcagcagcg ggccattgtg gaagccggcg
     1801 gcctcgcggc cgatgccggt cacctgctcc tggatgccct tgccgccgac cgggactccg
     1861 agcccggggg tcggcggggt gacctgcgcg gccggcatcg catgagccga atgatcgact
     1921 gccccggtcc cggcagccgg ctgcgcggcc ggagcggcgg cctgcggagc agccgcctgc
     1981 ggagcctgcg ccgctgccgg agtgagcccg gccgcggcca ggacaatcgc ccatccaatc
     2041 aatttccttc acgaccccgg tgacatgggt ttgggactgg ggccgccgcc gcggggcccc
     2101 tcgatttggg cggccttata ggcgcggctt ttcccgccct caagcgccac ttccgccatc
     2161 cgcggcgcgg tgttgaagcg gccccgcgag cagcctattc acgcggccgc gaccgcatcg
     2221 gaaggacggc atgaccgacg acgagatcct cgatgagttc cgggcggccg aggccttgct
     2281 cgagggccat ttcatcctgt cgtccgggct tcgcagcccg cgctatctgc agtgcgcgcg
     2341 ggtgctgatg gacccggcgc gggccgggcg gctggcgagc gcgctcgccg ccaagcttcc
     2401 ggccgaactg cgcgacggta tcgacgcggt ggtctcgccg gcgatgggcg gggtcatcat
     2461 cggccacgag atgggccggg cgctgggcag gcgagcgatg ttcgtcgagc ggccgcaggg
     2521 cccgttcgag cttcgccgcg gctttaggct cgagccgggc gagcgcgtgc tgctggtcga
     2581 ggacgtggtc accaccggcc tgtcgtcgcg cgaggcgatc gcggccatca ccgaggcggg
     2641 cggtcgggtg gtcggcgccg cggccctggt cgaccgctcg ggcggcaccg ccgacctcgg
     2701 cgtcccgctg acggcgctga tccggatcga cgtcccgacc tatgccgccg acgagcttcc
     2761 gcccgagctc gcctcgatcc cggcggtcaa gcccggaagc cgggcggccg cgtgagcagc
     2821 ccactccgtc tcggcgtcaa catcgaccat gtcgcgacca tccgaaacgc gcgcggcggc
     2881 gcccatcccg acccggtgcg cgcggccatc gccgccgcgg ccgccggcgc cgacggcatc
     2941 accgcccacc ttcgcgagga tcgccggcac atcaccgacg aggacatcga ccggctgatg
     3001 gggcagatcg acctgccctt gaacctcgaa atggcggcga ccgaggaaat gctggcgatc
     3061 gcgcttgcgc accgcccgca cgccgcctgc attgtccccg aaaagcgcga ggagcggacc
     3121 accgagggcg gcctcgacgc ggccgggcag cacaaccacc tggcttatta tgtcgaccgg
     3181 ctggggtccg cgaacatccg tgtcagcctg ttcatcgagc cccgcgagga gcaggtcgat
     3241 gccgcgcttc ggctcggcgc tccggtcgtc gagttccaca ccgggcgcta tgcccatgtg
     3301 gacggcgagg agcgcgcgac cgagctcaag cgcatcgcag actgtgccgc gctcgccgtc
     3361 aaggccggca tcgagccgca cgccggccac ggcctgacgt tcgacaatgt cgcgccggtc
     3421 gccgccatcc cgcagctcgc cgagctcaac atcggccatt tcctggtcgg cgaggcgatc
     3481 ttcaccggcc tcgaagcgag cgttcgccag atgcgcgagc tgatggacgc ggcgcggtga
     3541 agaaagaaac cctcaccctg ccctctcccg caagcgggag agggttggaa ggcacggtcg
     3601 catgctccct ctcccgcgtg cgggaggggg ttggggtgag ggcccttcct tgatcgtcgg
     3661 catcggctcg gacctttgca acatcgagcg gatcgagcgc tcgctggagc ggttcggcga
     3721 ccgcttcctc gagcgcgtgt tcaccgcgac cgagcgcgcc aaggcggatt cgcggccgtt
     3781 cacccgcgcc gggacgctcg ccaagcgctt cgccgccaag gaggccttct ccaaggcggt
     3841 cggcaccggc ttcaagcacg gcgtgttcat gaaggacatc ggcgttgtca atgcgccttc
     3901 gggagcgccg actctcgagc tgaccggcgg cgcccgcgca actcttgacg cccttgcgcc
     3961 ggcgggccac gccatcgaca ttcacttgac cctgaccgac gaatatccct gggcgcaggc
     4021 cttcgtcatc ctgaccgcac gcaagatgga gccctgacct gaccgacacc atccccgagg
     4081 cgaccaccga agcatcctcg ccgcgcgccg aacacaagga gtcgaagggc tcggccgtgt
     4141 ggcgcgagat caagggctta ctgtgggtgc tggtcgcggt gctcgccttc cacagcttcg
     4201 tcgccaagcc gttctacatc ccatcggaat cgatgatgcc gggcctgctc aagggcgacc
     4261 ggctggtggt cagcaaatat ccgtacggct ggtcgtgggt cagcccgagc ttccatgtct
     4321 tcccgcactg ggagggccga ctgctcggcg cgatgccgaa gcgcggcgac atcgtcatcg
     4381 tcactccgcc gggccagagc gacgactaca tcaagcgggt gatcggcctg cccggagaca
     4441 ccgtcgaggt gcgccgcggg cggctgatcc tgaacggccg gccggtgcgc tcggaagtcc
     4501 ggccctcggc gctgatcccg gtcgacccca acgtcccgtg cgggatcgag ttctccggct
     4561 tcgtcgtgcg ctcgggcggc cgcgaatatt gccgcctgcc gatcgtccgc gagacccttc
     4621 cgggcgggcc cagctacgac acggtcgacc tcggcgagag cccgggcgac gatttcggcc
     4681 cggtcaaggt gcccgccgac catgtcttca tgatgggcga caaccgcgac cgcagcgccg
     4741 acagccgctt cggcctcggc ccgcccgaga acggcctcgg cggcccggtg ccgtgggaga
     4801 atatcggcgg ccgcgccgag ttcatcacct tctcgctcga cggaacgacc cggatctacg
     4861 acccggtcag ctggttccgg gcgctgcgct cgggccgggc cgggcgctcg ctcaggggga
     4921 gcgccggcta gatggccgag ccgccgaccg tcgaaccgca cgtcgagcgg ccggggccgg
     4981 ccgagttccg cgacccgctg gtccgccgcg agctggctcg ggcgatggtc tggttcgggg
     5041 ccgcgctgct catcgccggg acgatcgtcc tcgcccagcc gctgctgctg atcgtcggcg
     5101 ggatgatctt cgcggtcatc ctcgacggcg gcacccggct cctcggccgc tatctgccga
     5161 tcgcgcgcgg atggcgcctg gcgctggtcg tgttcctcgg cctcggcttc gtcgcctggg
     5221 tattctatta cgccgggacg accttcgccg cccaggccga gaacctgcga gcggtcgtcg
     5281 aggtgcaagc ggcgcgagcc tatgcctggg cgcaggactt cggcctggtc tcggacccga
     5341 cccccggctc catcggccag cagctgatgg gctcggtcgg ccggctgacc actgccgtcg
     5401 gaagcgcgct cggcgcggtc accagcgcga tcctgatctt cgtcatcggc atcttcatcg
     5461 ccatcgaccc gcggctctac gaccgcggcg tcgcgtggat gcttccctta cggtaccgcg
     5521 accgcttcta cgcgatcggc gaccgggtcg gctggaccct tcgccggctg ctcgccgggc
     5581 ggctggtcgg gatgctgttc gagggcgtgt tcacctggct gatgctcggc tatgtgccgc
     5641 agctcttcgg cctggcgccg gtgccgatgg cggcgctgct cgggctgatc accggcctgc
     5701 tcgccttcgt ccccaacatc ggcgcgatca tctccggggt gctgatggtc gcggtcggct
     5761 tcagcgccgg ggtcgaccag gggctgtggt cgatcttcgt ctatttcctc gtccagaacg
     5821 tcgacggcta tctggtcatc ccctacatcg ccaagcgcac ggtcgacctc gccccggccg
     5881 tggtgctcgc ggcgcagctg ttgttcggcg cgctgttcgg cttcctcgga ctgctgctcg
     5941 ccgattcgat cctcgcggcg ctcaaggtca cgctccagga gctgagcaaa taccgcgccg
     6001 acgacgaggg cgaactgccc gaagtggcct agggctgcgg cgccggctgt tcgggcggct
     6061 gctgctcggg accctgctgg atgccctgca tcagcgccgc attgggcacg acctggacga
     6121 catggacgtc gaactcgagg tcggcgttgg gcggaatcgg gctgccgggc tgcggattgg
     6181 cgccgtagcc gagcgccgac gggatttgga tccggtagcg cccgcccttc tgcatccggg
     6241 tcagcgcctc ggcgaagccg gggatcacct ggccggcgat catcggcgtc gggccgcggc
     6301 cctcgctggt gtcgaacacc gtcccgtcct cgagccggcc ttcatattcg atcagcaccc
     6361 cgtcgaccgc cttgatcagc ggcccctcgc cggcctcgac ggtgcggaat ccaagcccgc
     6421 tggcggtggt ttcgccgcgc agcgagccgg cgccgagcca cgccaagccg acgccggcgg
     6481 caatcagcaa gaggatcgcc agccacagct tgccggttcc acgacggcgt tcgagatggg
     6541 cgagtgtgct ggcggacatc gatcgcctcc tcgtcgggcg gcttcaaacg ggaaggctag
     6601 cgggcggtgc cttcgcgctc ggcgcgcttg cgctcgagct tgcgggcgcg gcggatggcg
     6661 gcggcccgct cgcgggcgcg cttctccgag ggcttctcgt agtggcggcg cagcttcatc
     6721 tcgcggtaga cgccctcgcg ctgcagcttc ttcttgagcg cgcgcagcgc ctgctcgaca
     6781 ttattgtcgc gaacgatgat ctgcataaaa agccgtcaca ctcctgaatt ttcgcccaaa
     6841 tgcgccgaac gaccgcttgg cggcgcgcgg ccacgaaaaa ggtcgttcca atgcctgtta
     6901 ttcgccgcag gcgggcctac ggcggcgcgg ggccataaca gtgtccggac aagcgttcaa
     6961 gtcagtcact tttcagggaa ctttttcggt tattgcgacg agccgttgat ctggtgcggt
     7021 ggagagggca accgactgcg cttggttatc ggcgattaac cttccgcctg atacaccaca
     7081 cccttcttga gcggagggat ggatgtcgtt gaatcggagg gaaatgttgc gtcttggcgc
     7141 cgtcggcgtc gggggtgcga tgctgtcctc cgcggccagc tcggtcggtc ttccctcggt
     7201 cgtgttccag gccccgcgtt ccccgctgcc gccggttccg gccgctccgc gcgctccgta
     7261 cggggtcgac cagcgcattt tcgagcgggc ccgggcggcg ctcgatgccc atcgcatcta
     7321 tcaccgcgac ttcatcggca tcgccgactt ctcgctcccg tcgagcgatc cgcgcttcca
     7381 cgtcgtcgac ctgcgcaacg gccaggtcga aagctaccgg gtcgcgcacg gccagggctc
     7441 ggacccgaac cacaacggct tcctcgaccg cttctcgaac gagttcggct cctacgcgac
     7501 gtcgaacggc acctacacga ccggtgaata ttatcacggc aagtacggcc tctcgatgaa
     7561 gacccacggc cacgactggt cgaacagcaa tgccgagccg cgggcgatcg tcatccacaa
     7621 cgcctg
//
LOCUS       DASTZO010000264         5300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_99670,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000264 DASTZO010000000
VERSION     DASTZO010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99670"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..650
                     /locus_tag="VFU20_07415"
     CDS             <1..650
                     /locus_tag="VFU20_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020372191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative 2OG-Fe(II) oxygenase"
                     /protein_id="HEU5286320.1"
                     /translation="VYDIADRLPPLGTLADALRALHTTKGQPLVQSVRGGTQTDGHLF
                     HLIDPTIVALREAIAEVVREHVARLPARDPAHPLLGSPREPIAFSGAWSVRLQAGGFH
                     SNHVHPMGWISSALYIVLPPDLGRDQSGGAQSAGDQSGGGQAGWLTLGEPRTSSFPLD
                     LPPTRLVEPRPGRLALFPSWMWHGTRPFGAGERLTVAFDVAVPGANRASRASPGT"
     gene            741..1004
                     /locus_tag="VFU20_07420"
     CDS             741..1004
                     /locus_tag="VFU20_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286321.1"
                     /translation="MLKPLIIAALAVTAASPAVADTPPAAAKTAEKAKDPNRKICEKI
                     EKIGSRLNVVRVCMTAAQWDEQRRENREHFERVQRVVNQSPSN"
     gene            1090..1344
                     /locus_tag="VFU20_07425"
     CDS             1090..1344
                     /locus_tag="VFU20_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286322.1"
                     /translation="MLRPFLMLALLITAAPALALDAAAPPAAAKAKDPKRKICEPVEE
                     TGSRLSSKRICLTAEQWEAQRGGKSAAKRPVKSSGNGGGR"
     gene            complement(1346..2788)
                     /locus_tag="VFU20_07430"
     CDS             complement(1346..2788)
                     /locus_tag="VFU20_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEU5286323.1"
                     /translation="MNEPAWQSLLKEASALREAGRVDEAIAAYQRLLAANPALPGSWY
                     NLGWLQKQARRFEEALRSYAEALERGAGDPEEVHLNRAVILSDHLGRPEDALEELEAA
                     LALNPDYVPALLNLGNLHEDRGDREAAREAYRRALELDPGSALALARLAGVGVPDDRL
                     IERLESAIAGPAAPAEKADLGFALGRLLDQAGDYDGAFAAYAAANRASAQSHGPGFRG
                     YDRRAHEQFVDRLIAAFPHAAEPRGSSEGRSPVFICGLFRSGSTLVEQILAAHSRVSA
                     GGELDLIPALAARIRPYPEAVASADPAALEDLRRAYLAGMPAAVPGTLVTDKRPDNFL
                     HIGLIKAMFPEARIVHTRRNPLDNLLSLYFLHLDPGMAYALDLGDAAHWHGEYRRLMA
                     HWKAIHGADIHDVDYDELVRDPRPAIERLLEFCGLEWEESCLAFHSAPRVVKTASVWQ
                     VREPLYGRSSGRWKNYERHLRPLVDALEGL"
     gene            complement(2831..>5300)
                     /gene="clpB"
                     /locus_tag="VFU20_07435"
     CDS             complement(2831..>5300)
                     /gene="clpB"
                     /locus_tag="VFU20_07435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEU5286324.1"
                     /translation="MAAGLIEAAGGDPKAAQRAVDELVARVPAVTGSGATSAPALDGD
                     TIRILDQAEQVAKKSGDSYVTVERILLAMALAKTTDVGKALAGAGVTPQALNAAIDKL
                     RGGRSADTQSAEDRYDALKRYTRDLTQAARDGKLDPVIGRDEEIRRTIQVLARRTKNN
                     PVLIGEPGVGKTAIAEGLALRIANGDVPDGIKDRRLLSLDMGSLIAGAKYRGEFEERI
                     KGVLDEVKQAAGEIILFIDEMHTLVGAGKSEGAMDASNLLKPALARGELHCIGATTLD
                     EYRKHVEKDAALERRFQPVFIGEPTVPDTISILRGLKEKYELHHGVRITDAAIVAAAM
                     LSNRYISDRFLPDKAIDLMDEAASRIRMEVESKPEEIENLDRRIIQLKIEREALKKES
                     DKASKERLAKLEGELANLEQQSAELTQRWQAEKDKIASEAKLKEQLDQARIELEQAQR
                     GGDLARAGELQYGRIPELEKQLSEAQAATHGAMLREEVTGEDIAAVVSRWTGIPVERM
                     MEGEREKLLQMEAAIGARVIGQEEAVKAVASAVRRARAGLQDPNRPLGSFLFLGPTGV
                     GKTELTKALAEFLFDDSSAMVRIDMSEFMEKHAVARLIGAPPGYVGYEEGGVLTEAVR
                     RRPYQVVLFDEVEKAHGDVFNVLLQVLDDGRLTDGQGRTVDFTNTIIILTSNLGSQYL
                     AALTEDEEVSKVEPQVMEVVRAHFRPEFLNRLDEIILFHRLSAGHMGPIVDIQVARLQ
                     QLLDDRKIRIDLTDGARAWLGRVGYDPVYGARPLKRAVQKHLQDPLADRILAGEIPDG
                     SVVRVEEGDGALELTVGGERREAA"
BASE COUNT          715 a         1930 c         1805 g          850 t
ORIGIN      
        1 gggtctatga catcgccgac cggctgccgc cgctcggcac gctcgccgac gcgctccggg
       61 cgctgcacac gaccaagggg cagccgctgg tgcagtcggt gcgcggcggc acccagaccg
      121 acggccatct gttccacctg atcgacccga ccatcgtcgc cctgcgcgag gcgatcgccg
      181 aggtcgtccg ggagcatgtc gcccggctgc cggcccgcga cccggcccac ccgctgctcg
      241 gcagcccacg cgagccgatc gctttctccg gcgcctggtc ggtgcggctg caggccggcg
      301 gcttccattc caaccatgtc catccgatgg gctggatcag ctcggccctg tacatcgtgc
      361 ttccgccgga cctcggccgg gaccagtcgg gcggggctca gtcggccggg gaccagtcgg
      421 gcggcggcca ggccggctgg ctgacactcg gcgagccgcg cacctcgtcc ttcccgctcg
      481 acctgccgcc gacccggctg gtcgagccga ggcccggccg actcgccttg ttcccgtcgt
      541 ggatgtggca cggaacgcgg ccgttcggag ccggcgaacg gctgaccgtc gccttcgacg
      601 tcgccgtccc cggagcgaac cgggcgagcc gggcgagccc cgggacttga cgcgcatcgc
      661 gcacaatgat tgagttttcg gcgattgccg gccgcgagcc ctcgcgcatt gcgtacggct
      721 gccggcgagg agtttcgaac atgctgaagc cgctgatcat cgccgctctc gcggtcaccg
      781 ccgcgtcgcc cgccgttgcg gacacgccgc ccgccgccgc caagaccgcc gagaaggcca
      841 aggatccgaa ccggaaaatc tgcgagaaga tcgaaaagat cggctcgcgc ctcaacgtcg
      901 tccgggtctg catgacggcc gcccaatggg acgagcagag gcgcgagaat cgcgagcatt
      961 tcgagcgcgt gcagcgggtg gtcaaccagt ccccgtcgaa ctagcagctc gccccaccga
     1021 cccgattctc agcccggttg ccggccgacc cctcgattgc cggccgcata ttcttccagg
     1081 agttccccaa tgcttaggcc gttcctgatg ctcgctcttc tgatcaccgc cgccccggcg
     1141 ctggcgcttg atgcggccgc gcccccggcc gccgccaagg ccaaggaccc gaagcgcaag
     1201 atctgcgagc ccgtcgagga aaccggctcg cggctgagct cgaagcgcat ctgcctgacc
     1261 gccgagcaat gggaggccca gcgcggcggc aagagcgcgg ccaagcgccc ggtgaaatcc
     1321 tccggcaacg gcggcggccg ataggctaaa ggccttcgag cgcgtcgacg agcggccgca
     1381 ggtgccgctc gtaattcttc cagcgccccg acgagcggcc gtagagcggc tcgcgcacct
     1441 gccagacgct cgcggtcttc accactcgcg gcgccgagtg gaacgcgagg cacgattcct
     1501 cccattccag gccgcagaat tcgagcaatc gctcgatcgc cggccgcggg tcgcggacca
     1561 gctcgtcata gtcgacgtcg tggatgtcgg cgccgtggat cgccttccag tgggccatca
     1621 gccgccggta ctcgccatgc cagtgggcgg cgtccccaag gtcgagcgcg taggccatgc
     1681 ccgggtcgag gtggaggaaa tagagcgaca gcaggttgtc gagcgggttg cgccgggtgt
     1741 ggacgatccg cgcttcgggg aacatcgcct tgatgaggcc gatgtggagg aaattgtcgg
     1801 ggcgcttgtc ggtgacgagg gtcccgggca cggccgccgg cattccggcg agataggcgc
     1861 ggcgcaggtc ttccagcgcg gccggatcgg ctgaggcgac cgcctcggga tagggccgga
     1921 tccgggcggc gagcgccggg atcaggtcga gctcgccgcc ggcgctcacc cggctgtggg
     1981 cggcgaggat ctgctcgacc agcgtcgagc ccgaacggaa cagcccgcaa atgaacacag
     2041 gcgaccggcc ctcgctcgaa ccgcgcggct cggccgcatg cgggaaggcg gcgatcagcc
     2101 ggtcgacgaa ttgctcgtgc gcgcgccggt cgtagccgcg gaaaccgggg ccgtggcttt
     2161 gggcgctggc ccggttggcg gcggcatagg ccgcgaacgc gccgtcataa tcgccggcct
     2221 ggtcgagcag ccggccgagc gcgaagccga ggtcggcctt ttccgcgggc gccgccgggc
     2281 cggcgatcgc cgattccagc cgctcgatca gccggtcgtc ggggacgccg acccccgcca
     2341 gccgggcgag cgccagcgcg cttcccggat cgagctcgag cgcgcgccgg taggcctcgc
     2401 gcgccgcctc ccggtcgccg cggtcctcgt gcaggttgcc caggttgaga agcgccggga
     2461 catagtccgg gttgagcgcc agcgccgcct cgagctcctc gagcgcgtcc tcgggccggc
     2521 cgagatggtc ggacaggatc accgcccggt tcagatgcac ttcctcggga tcgccggcgc
     2581 cgcgctccag cgcctcggcg taggagcgca gcgcctcctc gaaccggcgc gcctgctttt
     2641 gcagccagcc gagattgtac cagctgccgg gcagggccgg gttcgcggcc agcagccgct
     2701 ggtaggcggc gatcgcctcg tcgacccggc ccgcctcgcg cagcgccgac gcctccttca
     2761 gcagcgattg ccaggccggt tcattcatcg ccgaggctta tcgcggcgcc ccgccttcga
     2821 gtcgaggcgc tcaggccgcc tcgcgccgct cgccgccgac cgtcagctcg agcgccccgt
     2881 cgccttcctc gacgcgcacc accgacccgt ccgggatttc gccggcgagg atcctgtcgg
     2941 cgagcgggtc ctgcagatgc ttctggaccg cgcgcttgag cggccgggcg ccgtagaccg
     3001 ggtcgtagcc gacccgcccg agccaggcgc gggcgccgtc ggtgagatcg atccggatct
     3061 tgcggtcgtc gagcagctgc tgcagccgcg cgacctggat gtcgacgatc ggccccatgt
     3121 ggccggccga cagccggtgg aacaggatga tctcgtcgag ccggttcagg aactcgggcc
     3181 ggaagtgggc ccggaccacc tccatcacct gcggctcgac cttcgacacc tcctcgtcct
     3241 cggtcaaagc ggccagatac tggctgccga ggttcgaggt caggatgatg atggtgttgg
     3301 tgaaatcgac cgtgcgcccc tggccgtcgg tcagccggcc gtcgtcgagc acctgcagga
     3361 gcacgttgaa cacgtcgccg tgggccttct cgacctcgtc gaacagcacc acctggtagg
     3421 gccgccgccg aaccgcctcg gtcagcacgc cgccttcctc atagccgacg tagcccggag
     3481 gggcgccgat cagccgcgcg accgcatgct tttccatgaa ctccgacatg tcgatccgga
     3541 ccatcgcgct cgaatcgtcg aacaggaact cggccagcgc cttggtcagc tcggtcttgc
     3601 cgaccccggt cgggccgagg aacaggaacg agcccaatgg ccggttcggg tcctgcaagc
     3661 ccgcccgggc gcggcgcacc gcggaagcga ccgccttcac cgcctcttcc tggccgatca
     3721 cccgggcgcc gatcgccgct tccatctgca gcaatttctc gcgctcgccc tccatcatcc
     3781 gctcgaccgg gatgccggtc cagcggctga ccaccgcggc gatgtcctca ccggtgacct
     3841 cctcgcggag catcgcgccg tgggtcgccg cctgggcttc ggagagctgc ttctcgagct
     3901 cggggatgcg cccgtactgg agctcgccgg cgcgggcgag gtcgccgccg cgctgcgcct
     3961 gctcgagctc gatccgcgcc tggtcgagct gctccttcaa tttcgcctcg ctggcgatct
     4021 tgtccttctc ggcctgccag cgctgggtca gctcggccga ctgctgctcg agattggcga
     4081 gctcgccctc gagcttggcc agccgctcct tcgatgcctt gtcgctttcc ttcttcagcg
     4141 cctcgcgctc gatcttcagc tggatgatcc gccggtcgag gttctcgatc tcctcgggct
     4201 tggattcgac ctccatccgg atgcggctcg ccgcctcgtc catcaggtcg atcgccttgt
     4261 cgggcaggaa gcggtcggaa atgtagcggt tggacagcat cgccgcggcg acgatcgccg
     4321 catcggtgat cctcaccccg tggtggagct cgtatttctc cttgaggccg cgcaggatcg
     4381 agatggtgtc gggcaccgtc ggctcgccga tgaacaccgg ctggaagcgc cgctccagcg
     4441 ccgcgtcctt ctcgacatgc ttgcggtact cgtcgagggt cgtcgctccg atgcagtgca
     4501 attcgccgcg cgccagcgcc ggcttcaaga ggttcgacgc gtccatcgcg ccctcgctct
     4561 tcccggcgcc gaccagggtg tgcatttcgt cgatgaacag gatgatctcg ccggccgcct
     4621 gcttgacctc gtcgagcacg cccttgatcc gctcctcgaa ctcgccgcgg tatttcgcgc
     4681 ccgcgatcag cgagcccatg tcgaggctga gcagccgccg gtccttgatc ccgtcgggga
     4741 cgtcgccgtt ggcgatcctg agcgcgagcc cttcggcgat cgcggtcttg ccgacccccg
     4801 gctcgccgat cagcaccggg ttgttcttgg tccgccgggc cagcacctgg atggttcggc
     4861 ggatttcctc gtcgcggccg atcaccgggt cgagcttgcc gtcgcgcgcc gcctgggtga
     4921 ggtcgcgggt atagcgcttg agggcgtcgt agcggtcctc ggccgactgg gtgtcggcgc
     4981 tgcgcccgcc gcgcagcttg tcgatcgccg cgttgagcgc ctgcggggtg acgcccgcgc
     5041 cggccagcgc cttgccgacg tcggtcgtct tggcgagcgc catcgccagc aggatccgct
     5101 cgacggtcac gtaactgtcg cccgatttct tggcgacctg ctcggcctgg tcgaggattc
     5161 ggatggtgtc gccgtcgagc gccggggccg aggtcgcgcc gctgccggtc accgccggca
     5221 ccctggcgac cagctcgtcg accgcgcgct gcgccgcctt ggggtcgccg cccgccgcct
     5281 cgatcaggcc cgcggccatc
//
LOCUS       DASTZO010000265         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_518574,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000265 DASTZO010000000
VERSION     DASTZO010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518574"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1516)
                     /locus_tag="VFU20_07440"
     CDS             complement(<1..1516)
                     /locus_tag="VFU20_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5286325.1"
                     /translation="MATIAQPSEAKGEKPRQSFAGLWNISFGFFGIQIGFALQNANMS
                     RIFQSLGSSLDDLPALWIAAPLTGLLVQPIVGHMSDRTWLGRLGRRRPYFLAGAILAA
                     LSLFLMPLAPVLLMAAILLWTLDASLNISMEPFRAFVGDMLRKDQHTAGYAVQTAFIG
                     AGAVVGSIFPWLLESIGVANEVPGGGIPDTVRYSFWFGGGALFLAVLWTIVSTREYSP
                     DEMAAFTESVDHPDSSQTLRALAARGLGGSLVWIAAGALVLLSVVQFGLEKEVYLLGG
                     LLIAYGLASIIAILLARSGSTTNMLSHIVGDFSGMPTVMKRLAVAQFFSWSALFIMWI
                     NTTPVVAQDFYGSGDPASAGYQAAGNWVGVLFAIYNGVAAIAALTLLPWLAARVGKAR
                     THMICLLAGSAAYASFLFIRDPQWLIASEIGVGIAWASILAMPYAILAGSLPQRKLGI
                     YMGLFNVFIVIPQLLVATVMGSIMKAFFPGEPIWTMLAAAIVMALAALAMLRVDE"
     gene            1720..>2391
                     /locus_tag="VFU20_07445"
     CDS             1720..>2391
                     /locus_tag="VFU20_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5286326.1"
                     /translation="METDATAPPNAAQLRWYVFGLFFIFGGITSLNDVLIPKLKHLFA
                     LNYFEAMLIQFAFFMAYALISMPGAWLVKRYGYMRTAVIGLVTMAAGCLLFIPASLSS
                     LYVAFLAALFVLASGITIVQVVANPLISMLGPKKTTHSRLTFAQAFNSLGTTIFPIVG
                     ATLILGSLAAVDPASLSGPALDEFLTRETQAIVRTYLGLAAALGLLAFLVYLGRNRLV
                     ETPAPA"
BASE COUNT          417 a          825 c          777 g          372 t
ORIGIN      
        1 cctcgtcgac ccggagcatc gccagtgccg caagcgccat gacgatcgcc gcggcgagca
       61 tcgtccagat cggctcgccc gggaaaaacg ccttcatgat cgaccccatc accgtcgcga
      121 ccagcaattg ggggatgacg atgaagacgt tgaacaggcc catgtagatg ccgagcttgc
      181 gctgcggcag gctgccggcg aggatcgcgt agggcatggc gaggatcgac gcccaggcga
      241 tcccgacccc gatctccgag gcgatcagcc attgcgggtc gcggatgaac aggaagctcg
      301 cataggcggc cgagcccgcg agcaggcaga tcatgtgggt gcgcgccttg ccgacccgcg
      361 ccgcgagcca cggcagcagg gtcagggcgg cgatcgccgc gacgccgttg tagatcgcga
      421 acagcacccc gacccaattg cccgctgcct ggtagccggc gctcgccggg tcgccggagc
      481 cgtagaaatc ctgggcgacg accggcgtgg tgttgatcca catgatgaac agcgccgacc
      541 agctgaagaa ttgcgcgacc gcgagccgct tcatcaccgt cggcatgccc gagaaatcgc
      601 cgacgatgtg gctcagcatg ttggtcgtgc tgcccgagcg ggcgagcagg atcgcgatga
      661 tgctcgcaag gccgtaggcg atcagcaggc cgccgagcag atagacttcc ttctcgagcc
      721 cgaactgcac caccgagagc aggaccagcg ccccggcggc gatccacacc aggcttccgc
      781 cgagcccgcg cgcggcgagc gcgcggagcg tctgcgagct gtccggatgg tcgacgcttt
      841 cggtgaaggc cgccatctcg tcgggcgaat attcccgggt cgagacgatc gtccacagca
      901 ccgcgaggaa cagcgccccg ccgccgaacc agaagctgta gcggacggtg tcggggattc
      961 cgccgccggg cacctcgttg gcgacgccga tcgattcgag cagccagggg aagatcgagc
     1021 cgaccaccgc cccggcgccg atgaacgcgg tctggaccgc atagccggcg gtgtgctggt
     1081 ccttgcgcag catgtcgccg acgaaagccc ggaacggctc catcgagatg ttgaggctgg
     1141 cgtccaatgt ccacagcagg atcgcggcca tcagcagcac cggcgccagc ggcatcagga
     1201 acagcgacag agcggcgagg atcgctccgg cgaggaaata gggccgccgc cgcccgagcc
     1261 ggccgagcca ggtgcggtcg gacatgtggc cgacgatcgg ctgaacgagg aggccggtca
     1321 gcggcgccgc gatccacagc gccggcaaat cgtcgaggct cgagccgagc gattggaaga
     1381 tccgcgacat gttcgcgttc tggagcgcga agccgatctg gatcccgaag aagccgaacg
     1441 agatgttcca caggccggcg aacgactgcc gcggcttctc ccctttggcc tcgctgggct
     1501 gcgcaatggt cgccaccggt ctctccccac cttcgatgcc gcgcgaagct ggcagaactg
     1561 ccggtgcccg acaacgcccg attgcccttt gtgcagtgca gcgcatacga atacgctggt
     1621 gacgaaccgc agggcggcgg ttagccatcc ggacgcaaca gggcaggggg aacttgcgtg
     1681 gcgctttcac cgacacatga cgaagcgacg gtcagtccga tcgagacgga tgcgaccgct
     1741 ccgcccaacg ccgcccagct gcgctggtac gtgttcggcc tgttcttcat cttcggcggg
     1801 atcaccagcc tcaacgacgt gctgatcccc aagctcaagc acctgttcgc cctcaattat
     1861 ttcgaggcga tgctgatcca gttcgccttc ttcatggcct atgcattgat ctcgatgccc
     1921 ggcgcctggc tggtcaagcg ctacggctac atgcgcaccg ccgtcatcgg gcttgtcacc
     1981 atggccgccg gctgcctgct gttcatcccg gcctcgctgt cgtcgctgta cgtcgccttc
     2041 ctcgccgctt tgttcgtgct ggcgagcggg atcaccatcg tccaggtggt cgccaacccc
     2101 ttgatctcga tgctcggccc gaagaagacc acccacagcc ggctgacctt cgcccaggcg
     2161 ttcaactcgc tcggcacgac gatcttcccg atcgtcggcg cgaccctgat cctcggctcg
     2221 ctggcggcgg tcgacccggc gagcctgagc gggccggcgc tcgacgagtt cctgacccgg
     2281 gagacgcagg cgatcgtccg cacctatctc ggcctcgccg cggcgctcgg cctgctcgcc
     2341 ttcctcgtct atctcggccg caaccggctt gtcgaaacgc ccgcgccggc g
//
LOCUS       DASTZO010000266         3302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_230374,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000266 DASTZO010000000
VERSION     DASTZO010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230374"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(60..1604)
                     /locus_tag="VFU20_07450"
     CDS             complement(60..1604)
                     /locus_tag="VFU20_07450"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF019205.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5286327.1"
                     /translation="MTSAAAQRFAALPTAAKLLLILTAALLPIGVALVWIAEIESRGA
                     DRASRELTEDQARTAARAVESLIARNALALRIAANGALAGGPDQACDRARRSLAVAPG
                     VAHEFELSAPDGTPLCAVGTVGAPPDFTLVAPGDIRLRVAPGGDALNLRVGVVGGMAT
                     TLLPAAEMRAAALEQNRGIESLVVRDRERQLRLVEAASANDDHRAHSFSEWPLGNGQL
                     VARIGAQVPQITTVERLLLLLPLLMWVMAALISWVLVTRLLIRPLRRLERAVTRLQPG
                     ESANVLPDKLGPATEIQELRDAFARGLMRIEQSEHEMAEALDGQRRLVREVHHRVKNN
                     LQVVASLLNIHGRTAESAEARAAYEAIGRRVGALSIVHRNHFAEMEENRGIALRPLVS
                     ELAAELRGGAPASARGLTIDLDVETVHTTQDVAVSVAFLITEIIEYAMLHCPDDPVEV
                     SLRRISELTARLMLSSPVLVPDEDGTPDKLQFERVVGGLAKQLRSALDRKLGRYSVDL
                     PVFPDS"
     gene            complement(1629..1841)
                     /locus_tag="VFU20_07455"
     CDS             complement(1629..1841)
                     /locus_tag="VFU20_07455"
                     /inference="COORDINATES: protein motif:HMM:NF037848.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NepR family anti-sigma factor"
                     /protein_id="HEU5286328.1"
                     /translation="MSGRQQGESDGIGKRKSSSKSGRAGKSAPRDRGVRKQRASELGR
                     ALRSIYDDTLRESVPDDFMDLLGKLS"
     gene            2160..2957
                     /locus_tag="VFU20_07460"
     CDS             2160..2957
                     /locus_tag="VFU20_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5286329.1"
                     /translation="MSLGQELAPHLPFLRRYARALTGSQAHGDAFVRATLEAIVAEPD
                     EFPRDVDPRLGLYRTFHAIWSTANIEEGEEPSRSFSGSEGIAQARLSRITPLSRQALL
                     LTSLEGFSSEDTAYLIGATPADVDSLVAEALSEIERQTLADVLIIEDEPIIAMDIETI
                     VRDLGHNVTGVAVTRDEAVAQARSNPPGLVLADIQLADDSSGIDAVKDILAEFSVPVI
                     FITAFPERLLTGTRPEPTFLITKPFQRSTVKAAIAQALFFDAATVPA"
     gene            3031..3177
                     /locus_tag="VFU20_07465"
     CDS             3031..3177
                     /locus_tag="VFU20_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286330.1"
                     /translation="MMSNERQEQVTTTEARAGVTPHMTRYVLGISLALVVVLFAIIYA
                     IAAT"
BASE COUNT          536 a         1124 c         1111 g          531 t
ORIGIN      
        1 gggttccgag catccgcaac attttttttc gacgcccccg caacgatttg tcggtttcgt
       61 caactgtctg gaaacacggg caaatcgacg ctgtagcggc cgagcttgcg gtccagcgcc
      121 gagcgcagct gcttggccag cccgccgacc acccgctcga actggagctt gtcgggcgtc
      181 ccgtcctcgt cgggaaccag caccgggctg ctgagcatca gccgggcggt cagctcgctg
      241 atgcggcgga gcgagacctc gaccgggtcg tccgggcagt ggagcatcgc atattcgatg
      301 atctcggtga tcaggaaggc gaccgacacc gcgacgtcct gggtcgtgtg gacggtctcg
      361 acgtcgaggt cgatggtcag gccccgcgcg gacgccggcg ccccgccgcg aagctcggcg
      421 gcgagctccg acaccagcgg ccgaagggcg attccgcgat tctcctccat ctcggcgaaa
      481 tggttgcggt ggacgatcga cagggccccg acccggcggc cgatcgcctc gtaggcggcg
      541 cgggcctcgg cggactcggc ggtgcggccg tggatgttga gcagcgaggc gacgacctgg
      601 aggttgttct tcacccggtg gtggacctcg cgcaccagcc gccgctgccc gtcgagcgcc
      661 tcggccatct cgtgctcgga ctgctcgatc cgcatcagcc cgcgggcgaa cgcgtcgcgc
      721 agctcctgga tctcggtcgc cgggccgagc ttgtcgggca ggacgttggc gctttcgccc
      781 ggctggagtc gggtgaccgc gcgctcgagc cgccgcagcg ggcggatcag aagccgcgtg
      841 acgaggaccc agctgatcag cgccgccatc acccacatca gcagcggcaa cagcagcaaa
      901 agccgctcga cggtggtgat ctgcggcact tgcgcgccga tccgggcgac cagctgcccg
      961 ttgccgagcg gccattcgct gaacgaatgg gcacgatggt cgtcgttggc ggacgcggcc
     1021 tcgaccagcc gcagttggcg ctcgcggtcg cggacgacca gggattcgat gccgcggttc
     1081 tgctcgagcg cggcggcgcg catctcggcg gccggcagca gggtggtcgc cattccccca
     1141 accaccccga cccgcaggtt cagagcgtcg ccgccgggcg cgacccgcag gcggatgtcg
     1201 cccggggcga cgagggtgaa gtccggcgga gctccgaccg ttccgaccgc gcacagagga
     1261 gtgccgtcgg gggcgctgag ctcgaactca tgagcgaccc ccggggccac ggccagcgag
     1321 cgccgcgccc ggtcgcaggc ctggtccggc ccgccggcga gcgctccgtt ggccgcgatc
     1381 cggagcgcca gcgcgttgcg ggcgatcagg ctttcgaccg cccgcgcggc cgtccgcgcc
     1441 tggtcctcgg tcagctcccg cgaagcccgg tcggcgccac ggctttcgat ttcggcgatc
     1501 cacaccaggg caactccgat cggcagcaag gcggcggtca ggatcagcag cagcttggcc
     1561 gccgtcggca gcgccgcgaa gcgctgggcg gcggcgctgg tcatgccacc gcccggactc
     1621 ggcccgtatc agctgagctt gccgagcagg tccatgaaat cgtcgggaac gctttcgcgc
     1681 agggtgtcgt catagatcga gcgcagcgca cggccgagct cggacgcccg ctgcttgcgg
     1741 actcccctgt cgcgcggcgc gcttttgccg gcgcgcccgg acttgctgga gcttttgcgt
     1801 ttgccgatgc cgtccgactc gccttgctgc ctgccactca acctctcgtc ccccctcacg
     1861 cgggtgcaag cgcgcgtctt gaaaacgcca aaacgtcacg cgcatcgggc ccgaaccggt
     1921 agtcgaacag cgcagcgaaa tgccccagcc ccgagccgtc cgcggcggcg atcgacactg
     1981 ccgaagacgg tgccgagccg cgatggaaac caaatcgccg gtgctttgtt ccagaggcgc
     2041 cgcacttctc tgtggactag tgcagagcgg caacggggtc gtccttccag ggttgcaaca
     2101 atcgggtggc gcgctcaaag agccgcgaaa catataaaga tcatggagtg cactcgctta
     2161 tgtcgcttgg ccaggaactt gctccgcatc tgcccttcct tcgccgctac gcgcgggcgc
     2221 tgaccgggag ccaggcccat ggcgacgcgt tcgtccgggc gaccctggaa gcgatcgtcg
     2281 ccgaaccgga cgagtttccg cgcgacgtcg acccgcggct gggcctctac cggacgtttc
     2341 acgcgatctg gtcgaccgcc aacatcgagg aaggcgagga gccgtcgcgc agcttttccg
     2401 gaagcgaggg catcgcccag gcgcgactgt cgcgaatcac gcctctgtcg cgccaggcgc
     2461 tgctgctgac ctcgctcgag ggcttttcgt ccgaggacac cgcttatctg atcggcgcca
     2521 ctccggccga cgtcgactcg ctggtcgccg aggcgttgag cgagatcgag cgccagacgc
     2581 tcgccgacgt gctgatcatc gaggacgagc cgatcatcgc gatggacatc gagaccatcg
     2641 tccgcgacct cggccacaac gtcaccgggg tcgccgtgac ccgcgacgaa gcggtcgccc
     2701 aggcgcggtc caatccgccg gggctggtgc tcgccgacat ccagctcgcc gacgattcga
     2761 gcggaatcga cgcggtgaag gacatcctcg ccgagttctc ggtgcccgtg atcttcatca
     2821 cggcgttccc ggagaggctg ttgaccggaa cccgcccgga acccaccttc ctaatcacca
     2881 agccgttcca gcggtcgacg gtgaaggcgg ccatagcgca ggcgttgttc ttcgacgcag
     2941 caacggtgcc ggcctgaccc ttaagccgta acgctcgcgg aacatgggct gacaaagcgc
     3001 gttggcctgt ggaacgcatg gcttgagaga atgatgagca acgagagaca ggaacaggtt
     3061 acgaccactg aagcgcgcgc aggcgtaacg cctcacatga cgcgctacgt tctcgggatc
     3121 tcgctggcgc tggtggtggt gttgttcgcg atcatttacg cgatcgcggc gacctgacct
     3181 ccatcgattc tgtcattctc tacgtgtatt catccggttc atattgagcc ggatacccat
     3241 tgtctgaacc gggcggggat tagtggccga agaacaagat caacaaacgg ccgaagaggc
     3301 gg
//
LOCUS       DASTZO010000267         4506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_545051,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000267 DASTZO010000000
VERSION     DASTZO010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4506
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545051"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3313)
                     /locus_tag="VFU20_07470"
     CDS             complement(<1..3313)
                     /locus_tag="VFU20_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HEU5286331.1"
                     /translation="MDRVWTFAIDRGGTFTDVVATASDGRRRVEKLLSENPHYPDAAV
                     EGIRRVLGAESGTLGEVRMGTTVATNALLERKGERLALAITRGFGDALRIGYQARPEI
                     FARQIVLPSMLYETVVEIDERVGANGEVLVALDEAAVRSGLERARSAGIDALAIVLMH
                     GWAFPAHEARLAEIARELGFTQVSVSHEVAPLIKLVGRGDTTVVDAYLSPVLRRYVDR
                     VAAGLGHAAALHFMQSNGGLAEARSFRGKDTILSGPAGGVVGMVAASAAHGGAKGQQL
                     LIGFDMGGTSTDVSHYAGRFELVDESVVAGVRIRAPMMQIHTVAAGGGSICRFDGMRF
                     RVGPQSAGARPGPACYRNGGPLTVTDCNLFLGRIEPGRFPAVFGPNGDQPLDPDASRA
                     RLLEVAEALGQAKSLEAIAEGFLEIAVDNMAAAIRKISIARGHDVSRYALACFGGAGG
                     QHACRVADALGMERILIHPLAGVLSAYGIGLAPVKAIREQSWLKPLGEDFSDGLRTLE
                     DAAREALVEQGVEPERIHLDHRARMRTPGSDTAFEVDVAAPAAMRTAFDALHRRRFGY
                     VEGSEPIVDALVVEAVSSSPEPERRSATPVEGSGETTEGPALIFDPTSTTVVEPGWSA
                     TRAGDGTLVLTRTTPARRAHAVGTEVDPVRLEIFNNLFMAIAEEMGVALQSTATSVNI
                     KERLDFSCALFDAGGALIANAPHIPVHLGSMGESIRTIIDSRGANRDGRGIRRGDAYV
                     LNDPYRGGTHLPDITVIVPVFYAGQDDAPSAFVAARGHHADVGGITPGSMPPGSRTIE
                     EEGVLIDNALLVDEGRFLEAETRALLASGPWPARNPDRNLSDLRAQLAACVRGAEALV
                     ETVRDYGADVVAAYMGHVLANAEESVRRLLDRLDDGAFDYEMDNGARIKVAIAIDKDS
                     RSATFDFTGTSAQLDDNFNAPFSIVRAASLYVARTLIDDAIPMNDGCLRPIRLVVPDG
                     SMLSPAFPAAVVAGNVETSQAVTDALFAATGRLAPSQGTMNNFTFGNDAHQYYETIAG
                     GSGAGPDHDGTSAVQTHMTNSRLTDPEILETRLPVRLDEFAIRRGSGGDGAHRGGDGV
                     VRRVTFLEPMRANILA"
     gene            3407..>4506
                     /locus_tag="VFU20_07475"
     CDS             3407..>4506
                     /locus_tag="VFU20_07475"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HEU5286332.1"
                     /translation="MYRVALTQSLFLAVRDRPVEKVTIGELLRRGAAAHADTIALKEL
                     GYDGAIGRTWTYAQLLADSERLGRALASRHAPGARIAVYANNVPEWVLLEFGSAMAGL
                     ILVTVNPSYQKRELKYVLEQSRSEGIYYVEEFRGTAMKAIADEVCAEIPAIGHRILLT
                     DHAALFDGADRGALPQVSPDDVTQIQYTSGTTGFPKGALLHHHGLVQNARDIMARCGL
                     RAGDTFLHIVPLFHTTGCAINVLGIIGAGATILLAPMFDPAMLVRAIERERPTFLLAV
                     PTMLVGLVDEAVRSGADVSSFRGIMSGGAVVAPELISRVKAVFGTIVQIVYGQTECSP
                     VISMVWKDDSMADLTGTIGQPLPNIDVAILDPG"
BASE COUNT          649 a         1549 c         1623 g          685 t
ORIGIN      
        1 tggcgaggat gttggcgcgc atcggctcga ggaaggtgac tcggcggacg acgccatcgc
       61 cgccgcggtg ggcgccgtcg ccgcccgagc cgcggcggat ggcgaattcg tcgagccgga
      121 ccggcagccg cgtctccagg atctcggggt cggtcaggcg gctgttggtc atgtgggtct
      181 gcaccgcgct ggtcccgtca tgatccggtc cggcgcccga gccgccggcg atggtctcat
      241 aatattggtg ggcgtcgttg ccgaaggtga aattgttcat cgtgccctgg gacggagcga
      301 ggcggccggt cgccgcgaac aaggcgtcgg tgaccgcctg gctggtctcg acgttgccgg
      361 cgacgaccgc ggccgggaag gcggggctga gcatcgatcc gtcggggacg accaggcgga
      421 tcggccgcaa gcagccgtcg ttcatcggga tcgcgtcgtc gatcagggtc cgcgcgacgt
      481 acagcgacgc cgcccggacg atcgagaaag gcgcgttgaa attgtcgtcg agctgggcgc
      541 tggtgccggt gaagtcgaag gtcgcggagc gcgagtcctt gtcgatcgcg atcgcgacct
      601 tgatgcgcgc gccgttgtcc atctcatagt cgaacgcgcc gtcgtccaga cggtccagca
      661 gccggcgcac cgactcctcg gcgttggcga gcacatggcc catgtaggcg gcgacgacgt
      721 cggcgccgta gtcgcgcacc gtctcgacga gcgcctcggc gccgcggacg caggcggcga
      781 gctgggcgcg caggtcggac aggttgcggt cggggttgcg cgccggccag gggccggagg
      841 cgagcagggc gcgggtctcg gcctcgagga accgcccctc gtcgaccagc agcgcattgt
      901 cgatcaatac gccctcttcc tcgatcgtcc ggctgccggg cggcatcgat cccggcgtga
      961 ttccgccgac gtcggcatgg tggccgcgcg cggcgacgaa cgcggacggc gcgtcgtcct
     1021 ggccggcgta gaacaccggg acgatcaccg tgatgtcggg caagtgcgtg cccccgcggt
     1081 acgggtcgtt gagaacataa gcgtcgccgc ggcggatccc gcggccgtcg cggttggcgc
     1141 cgcggctgtc gatgatcgtc cggatgctct cgcccatcga gccgaggtgc accgggatgt
     1201 gcggcgcgtt ggcgatcagc gcgcccccgg catcgaacaa ggcgcaggaa aaatcgagcc
     1261 gctccttgat gttgaccgag gtcgcggtcg actgcagcgc gacgcccatc tcctcggcga
     1321 tggccatgaa cagattgttg aagatctcga gccggaccgg gtcgacctcg gtgccgaccg
     1381 catgggcacg ccgggcgggc gtggttcggg tgaggaccag cgttccgtcg ccggcgcggg
     1441 tcgcgctcca gccgggctcg acgacggtgg tcgaggtggg atcgaagatc agcgccgggc
     1501 cttcggtggt ttcgccagat ccctcgaccg gcgtcgcgga ccttcgttcc ggttccggcg
     1561 aggagctgac ggcctcgacg accagcgcgt cgacgatcgg ctcgctgccc tcgacatagc
     1621 cgaagcggcg gcggtggagc gcgtcgaacg cggtgcgcat cgcggccgga gcggcgacgt
     1681 cgacttcgaa cgcggtgtcg ctgcccgggg tgcgcatccg ggcgcggtgg tcgaggtgga
     1741 tgcgctcggg ctcgacgccc tgctcgacca aagcctcgcg cgccgcatcc tcgagcgtcc
     1801 gcagcccgtc cgaaaaatcc tcgccgagcg gcttcagcca gctctgctcg cggatcgcct
     1861 tcaccggcgc gaggccgatc ccgtaggccg acagcactcc ggccaatggg tggatcagga
     1921 tgcgctccat gccgagcgcg tcggcgaccc ggcacgcgtg ctggccgccg gcgccgccga
     1981 agcaggcgag cgcgtagcgc gacacgtcgt ggccccgagc gatcgagatc ttgcggatcg
     2041 ccgcggccat attgtcgacc gcgatctcga ggaagccctc ggcgatcgcc tcgaggctct
     2101 tggcctgccc gagcgcctcc gcgacctcca gcagccgggc gcgcgacgcg tcggggtcca
     2161 gcggctggtc gccgttgggc ccgaacaccg ccgggaagcg cccgggttcg atccggccga
     2221 ggaacaggtt gcagtcggtg acggtcagcg gcccgccgtt gcggtagcag gcggggcccg
     2281 gccgagcgcc cgccgactgc ggaccgaccc ggaagcgcat gccgtcgaag cggcagatcg
     2341 acccgccgcc cgccgcgacc gtgtggatct gcatcatcgg cgcgcggatt cgaaccccgg
     2401 cgacgacgct ttcgtcgacc agctcgaagc ggccggcgta atgcgacacg tcggtcgagg
     2461 tgccgcccat gtcgaagccg atcaagagct gctgcccttt ggctccgccg tgcgccgcgc
     2521 tcgccgcgac cattcccacg accccgccgg ccgggccgga caggatcgtg tccttgccgc
     2581 ggaacgagcg cgcctcggcg agcccgccgt tcgactgcat gaaatggagc gcggcggcgt
     2641 ggccgagccc ggcggcgacc cgatcgacgt aacggcgcag cacgggcgac agataggcgt
     2701 cgacgacggt cgtgtcgccg cggccgacca gcttgatcag cggcgccacc tcgtggctga
     2761 ccgagacttg ggtgaagccg agctcacgcg cgatctcggc gagccgcgcc tcgtgggccg
     2821 ggaacgccca gccgtgcatc agcacgatgg ccagcgcgtc gatgccggcg gagcgcgcgc
     2881 gctcgagccc ggaacggacc gccgcttcgt cgagcgcgac cagcacctcg ccgttcgcgc
     2941 cgacgcgctc gtcgatctcg accaccgtct cgtacagcat cgacggcagc acgatctgcc
     3001 tcgcgaagat ttcgggccgc gcctggtagc cgatgcgcag cgcgtcgccg aagccccgcg
     3061 tgatcgccag cgccagccgc tcgcccttgc gctcgagcag cgcgttggtg gcgacggtgg
     3121 tgcccatccg cacttcgccg agcgtgccgc tctcggcccc gagcacccgg cggatgccct
     3181 cgaccgccgc gtcgggatag tgcgggttct cggacagcag tttctcgacc cggcggcggc
     3241 cgtccgacgc ggtcgcgacg acgtcggtga acgtcccccc gcggtcaatt gcaaaggtcc
     3301 agactcggtc cacggcgccc ttgaagcaaa tgcggcgatg cgcggccaat tatcttttga
     3361 cgcgcgcggc ggcgcggcgc aatttggcct cagggagggc tccgcgatgt atcgagttgc
     3421 gctgactcaa tccttgttcc tggccgtccg cgaccggccg gtcgagaagg tcaccatcgg
     3481 cgagcttctg cgccgcgggg cggctgcgca tgccgacacg atcgcgctca aggaactggg
     3541 ctacgacggc gcgatcggcc ggacctggac ctatgcgcag ctgttggccg attccgagcg
     3601 gctcggccgg gccctggcca gccgccacgc gcccggcgcg cggattgcgg tctatgcgaa
     3661 caatgtcccc gagtgggtgc tgctcgagtt cggctcggcg atggccgggc tgatcctagt
     3721 gacggtcaat ccgtcctacc agaagcgcga gctcaaatat gtgctcgagc agtcgcgctc
     3781 ggaaggcatc tattatgtcg aggagttccg cggcacggcg atgaaggcga tcgccgacga
     3841 ggtctgcgcc gagatcccgg cgatcgggca ccgcatcctg ctgaccgatc acgcggccct
     3901 gttcgacggc gcggaccgcg gcgcgctgcc gcaggtttcg cccgacgacg tgacgcagat
     3961 ccagtatacg tcggggacga ccggtttccc caagggcgcg ctgctccatc atcacgggct
     4021 ggtccagaat gcgcgcgaca tcatggcgcg ctgcggcttg cgcgccggcg acaccttcct
     4081 gcacatcgtg ccgctgttcc acaccaccgg ctgcgcgatc aacgtgctcg gcatcatcgg
     4141 cgccggggcg acgatcctgc tggcgccgat gttcgatccg gcgatgctgg tgcgggccat
     4201 cgagcgcgag cgcccgacct tcctgctcgc cgttccgacc atgctggtcg ggctggtcga
     4261 cgaagcggtg cgcagcggcg ccgacgtctc gtccttccgg ggcatcatgt cgggcggggc
     4321 ggtggtcgct cccgagctga tcagccgggt caaggcggtg ttcgggacga tcgtgcagat
     4381 cgtctacggc cagaccgaat gctcgccggt gatctcaatg gtgtggaagg acgattcgat
     4441 ggccgacctg accgggacca tcggccagcc gctgcccaac atcgacgtcg ccatcctcga
     4501 tcccgg
//
LOCUS       DASTZO010000268         4011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_884351,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000268 DASTZO010000000
VERSION     DASTZO010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4011
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884351"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(192..422)
                     /locus_tag="VFU20_07480"
     CDS             complement(192..422)
                     /locus_tag="VFU20_07480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286333.1"
                     /translation="MTILITAMALAAAPAPAAAQPAPAPAQAAHAHHSQPAQPGAKPE
                     GEGCSCCKKMAEGGKMECCAKHGKEAGEHSGH"
     gene            complement(462..788)
                     /locus_tag="VFU20_07485"
     CDS             complement(462..788)
                     /locus_tag="VFU20_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286334.1"
                     /translation="MAATILRLLTLAALLLMPFGMQTASATPVHHASAAATAAGHCDE
                     QGGQPAGRSPDQAVDCAMACSMLAAAEIRAGDAVPIRGLPLQRPLADPGAGLHPDTAT
                     PPPKLS"
     gene            complement(851..1153)
                     /locus_tag="VFU20_07490"
     CDS             complement(851..1153)
                     /locus_tag="VFU20_07490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286335.1"
                     /translation="MAAMTPDADEDEADRRLSMVDEALVAGTIANTNGLLVILAKLVA
                     HGVLDKDDLKTFSDSYSKPLDHVGMRENELVSQMQDQMESTLAELMRFLSERDRKK"
     gene            complement(1183..1680)
                     /locus_tag="VFU20_07495"
     CDS             complement(1183..1680)
                     /locus_tag="VFU20_07495"
                     /EC_number="3.2.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033958 - DNA-deoxyinosine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-deoxyinosine glycosylase"
                     /protein_id="HEU5286336.1"
                     /translation="MDSIKVGLPPVARPDARLFILGSLPGDASLSARRYYAHPTNQFW
                     RLLGQAIGEELQALGYDERLQRLAERRIGLWDVIASASRRGSLDQSIRGAAHNRVEQL
                     RHDFPELRALAFNGSTAAAAGRRLLGNAEGLALVELPSSSAANTRPFAQKAAAWAVLA
                     QWIDA"
     gene            1732..2901
                     /locus_tag="VFU20_07500"
     CDS             1732..2901
                     /locus_tag="VFU20_07500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEU5286337.1"
                     /translation="MQPSDLRIALFSGNYNYVRDGANQALNRLADYLLRQGAQVRAYS
                     PTVADPAFEATGDIVSVPSVPIPFRPEYRLPIALTPRVRRDLVEYRPNIVHIASPDIV
                     AHRAVSWARRRNIAAIASIHTRFDTYLSYYHLEIFEPAARAIMRRLYRRCDAIMAPAE
                     STAAVLRAQRMNRDISIWTRGVDREQFHPGRRDMGWRRGQGIDDDALVIAFLGRVVME
                     KGLDVFSDAVDALAERKVRHRVLVIGEGPARPWLEQRLPGAIFIGQQVGDDLARALAS
                     SDVLLNPSITEAFGNVTLESMACGLPVVAAVATGATSLVRDGETGVLVEPGDADAFAD
                     ALEAYASDPELRRRHGEAGLAYAQTMDWDRINSAVVRVYLRVIEKRARLARMTGR"
     gene            complement(2906..3538)
                     /locus_tag="VFU20_07505"
     CDS             complement(2906..3538)
                     /locus_tag="VFU20_07505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU5286338.1"
                     /translation="MTRRHWHGCGPRGRLEFNIPEGLFAMRGGRDWGGDWGPFHFDFG
                     DDSSGWRRGRRGRKRMFESGELRLVLLKLIADEPRHGYDLIRAIEELTGGEYAPSPGV
                     VYPTLTLLQDMGLIEEAQAEGPRKPFQATDEGRSHLEEKADEVEALFERLEEFAPRER
                     SSAGPSLGRAVSNLMNALRHRIGRDGFDEELVHEIATILDEAAQRIERVK"
     gene            3634..>4011
                     /locus_tag="VFU20_07510"
     CDS             3634..>4011
                     /locus_tag="VFU20_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5286339.1"
                     /translation="MADLFADQLPARRPEAPPANAPLADRLRPKSLGEVVGQEHLTGP
                     DGAIGRMVAAGKLSSMVLWGPPGTGKTTIARLLADAVGMRFVAISAVFSGVADLKKIF
                     AEARQMAKAGRHTLLFVDEVHRFN"
BASE COUNT          581 a         1424 c         1365 g          641 t
ORIGIN      
        1 ggcgtgctgg gcgggcgccg gcgcggacag ggcggcgagc gcgacggcgc tcgcggccag
       61 aaacaatttg atcttcacgg tcactccagg gctcggcgag gcgatattcg ccggatcgcc
      121 ggtttcggat cgaatgtggg aaaggaggcg ccgccggcaa gcggcgcccc ccggtcagct
      181 agccgccggc ctcagtggcc ggagtgctcg ccggcctctt tgccatgctt cgcgcagcac
      241 tccatttttc cgccttcggc cattttcttg cagcacgagc agccttcgcc ttccggcttg
      301 gcgcccggct gggccggttg cgaatggtgc gcatgagcgg cctgcgcagg agccggagcc
      361 ggctgggcgg cggccggggc gggagcagcg gcaagcgcca tcgcggtgat caggatcgtc
      421 attggaaaat ctcctggaaa tcgggttgtc ggaatcggat ttcaggagag cttcggaggc
      481 ggagtggccg tgtccgggtg aagtccggcg cccggatcgg cgagcgggcg ctgcaacggc
      541 aagcctcgga tcgggacggc atctccggcg cggatttccg ccgccgccag catcgagcag
      601 gccatcgcgc aatcgaccgc ctggtccggc gagcgcccgg cgggctgccc gccctgctcg
      661 tcgcaatgcc ccgccgcggt cgccgccgcc gacgcgtgat ggaccggagt cgcgctcgcc
      721 gtttgcatgc cgaatggcat cagcagcagc gcggccaggg tcagcagcct caggatcgtc
      781 gcggccatcg gcgcgattta ggcccgccaa ccttgactgg caatgactgg gcctggctcg
      841 acacgcccgc tcatttcttg cggtcgcgct cgctcaggaa ccgcatcagc tcggccaggg
      901 tcgattccat ctggtcctgc atctggctga ccagctcgtt ctcgcgcatc ccgacatggt
      961 cgagcggctt ggaatagctg tcggagaagg tcttcagatc atccttgtcg aggactccgt
     1021 gggcgaccag cttggcgagg atcaccagca ggccgttggt gttggcgatg gtcccggcga
     1081 ccagcgcctc gtcgaccatc gacagccgcc ggtcggcctc gtcttcgtcc gcgtcgggag
     1141 tcatggccgc catgttggcg cactcgccgc ccggggcaag tgctaagcgt cgatccactg
     1201 cgcgagcacc gcccaggccg cggccttttg cgcgaacgga cgcgtgttgg cggcgctcga
     1261 cgagggcagc tcgaccagcg ccagcccctc ggcgttccca agcagccgcc gcccggccgc
     1321 ggccgccgtc gagccgttga acgccagcgc ccgaagctcg gggaaatcgt ggcgaagctg
     1381 ctcgacccgg ttgtgcgcgg ccccgcggat cgactggtcg aggctgccgc ggcggctggc
     1441 cgaggcgatc acgtcccaca gcccgatgcg gcgctccgcc agccgctgca gccgctcgtc
     1501 atagcccagc gcctgcagct cctcgccgat cgcctggccg agcagccgcc agaactggtt
     1561 ggtcggatgc gcatagtaac gccgggcgga cagcgacgcg tcgccgggca ggctgccgag
     1621 gatgaacagc ctcgcgtcgg gccgggcgac cggcggcagt ccaaccttga tgctgtccat
     1681 tcgcccgccc ccgctttgcc tttgccgccg ccgccgttaa gggcatggcc ggtgcagccg
     1741 agcgaccttc gcattgccct gttttcgggc aattacaatt atgtgcgcga cggcgccaac
     1801 caggcgctca accggctggc cgactatctg ctgcggcagg gcgcccaggt ccgcgcttat
     1861 tcgccgacgg tggccgatcc ggcgttcgag gccacgggcg atatcgtcag cgtgccgtcg
     1921 gtgccgatcc cgttccggcc cgaatacagg ctgccgatcg cactgacccc gcgggtccgg
     1981 cgcgaccttg tcgaataccg gccgaacatc gtccacatcg ccagcccgga catcgtcgcc
     2041 caccgcgcgg tcagctgggc gcggcggcgc aatatcgccg cgatcgcctc gatccacacg
     2101 cgcttcgaca cctatctgtc ctattaccat ctggagattt tcgagccggc cgcgcgggcg
     2161 atcatgcgcc ggctctaccg ccgctgcgac gcgatcatgg cgccggcgga atcgaccgcc
     2221 gccgtgcttc gggcgcagcg catgaaccgc gacatctcga tctggacccg cggcgtcgac
     2281 cgcgagcagt tccaccccgg ccgccgcgac atgggctggc ggcgcggcca agggatcgac
     2341 gacgatgcgc tggtcatcgc cttcctcggc cgggtcgtga tggaaaaagg cctcgacgtg
     2401 ttctccgacg cggtcgacgc cctcgccgag cgcaaagtca ggcaccgggt gctggtgatc
     2461 ggcgagggcc ccgcccggcc gtggctcgag cagcgcctgc cgggcgcgat cttcatcggc
     2521 cagcaggtcg gcgacgacct cgcccgggcg ctcgccagca gcgacgttct gctcaacccg
     2581 tcgatcaccg aggcattcgg caacgtcacg ttggagtcga tggcgtgcgg gctgccggtg
     2641 gtggccgcgg tcgcgaccgg agcgaccagc ctggtgcgcg acggcgaaac gggagtgctg
     2701 gtcgagccgg gcgacgccga tgcgttcgcc gatgcgctcg aggcctatgc cagcgatccc
     2761 gagcttcgcc gccggcacgg cgaggcgggc ctcgcctacg cgcagacgat ggactgggac
     2821 cggatcaact cggccgtcgt ccgggtctat ctccgggtga tcgagaagcg cgcgcggctg
     2881 gcccggatga ccggccgcta gcgccttact tcacccgctc gatgcgctgc gccgcctcgt
     2941 cgaggatggt cgcgatctcg tgcaccagct cctcgtcgaa gccgtcgcgg ccgatgcgat
     3001 gccggagcgc gttcatcaga ttgctgaccg cccgccccag cgaaggtccg gcggagctgc
     3061 gctcgcgcgg agcgaactcc tcgagccgct cgaacagcgc ctcgacttcg tcggcttttt
     3121 cctcgagatg gctgcggccc tcgtcggtcg cctggaacgg cttgcgcggc ccttcggcct
     3181 gcgcttcctc gatcagcccc atgtcctgga gcagggtcag ggtcggatag accactccgg
     3241 ggctcggcgc atattcgccg ccggtcagct cctcgatcgc ccggatcaga tcgtagccgt
     3301 gccgcggctc gtcggcgatg agcttcaaga gcaccagccg gagctcgccg gattcgaaca
     3361 tccgcttgcg gccgcggcgg ccgcgccgcc agcccgatga atcgtcgccg aaatcgaaat
     3421 ggaacggtcc ccagtcgccg ccccagtcgc gcccgccgcg catcgcgaac aggccttcgg
     3481 gaatgttgaa ctcgagccgc ccgcgcggcc cgcagccatg ccaatgcctt cgtgtcatcc
     3541 acgcctcctc agatgtgtct aagatatatc ttcgatgtat cggcggcaag agcccccgtg
     3601 caatttcgcg gccggccatt atgagcggcg gcgatggccg acctgttcgc tgaccagcta
     3661 cccgcccgcc ggcccgaagc gcccccggcc aacgcgccgc tcgccgaccg gctgcgcccg
     3721 aaaagcctcg gcgaggtcgt cggccaggag catctgaccg gccccgacgg agcgatcggg
     3781 cggatggtcg cggcgggcaa attgtcgtcg atggtcctgt gggggccgcc cgggacaggc
     3841 aagacgacca tcgcccggct actcgccgac gcggtcggga tgcgcttcgt cgccatctcc
     3901 gcggtctttt ccggagtcgc cgacctcaag aagatcttcg ccgaggcccg gcagatggcg
     3961 aaggccggcc ggcacacgct cttgttcgtg gacgaggtgc accgcttcaa c
//
LOCUS       DASTZO010000269         9178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_962945,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000269 DASTZO010000000
VERSION     DASTZO010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 9178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9178
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_962945"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VFU20_07515"
     CDS             complement(<1..741)
                     /locus_tag="VFU20_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEU5286340.1"
                     /translation="MSAKVHLPGATAHEMTGWISRGALLAGAAFALPLQAQTVQPPAE
                     EPPGAPANGSQAPDEFAEDEAEIVVTGQRPRGSVIGDIPPENTLDARDVRATGATDIN
                     ELLEALSPQIGSARGRGGERPVLLLNGQRISSFRELRDIPTEAIQRVEILPEEVALKY
                     GYRADQRVVNIILRRRFRSTAARAEARAATEGGYAGGLGDLTRLMIGRDSRTTLNLRA
                     EGNGALTESERDIALQQLPENPDLDPRDA"
     gene            846..2429
                     /locus_tag="VFU20_07520"
     CDS             846..2429
                     /locus_tag="VFU20_07520"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010218722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU5286341.1"
                     /translation="MTNKPIALFAAIASLAGCAAASAEDPRFGGVPVATVAAPVAAPA
                     GPAEAATAAAIERIARIDPQLGSVLALDPTAIAQARRIDAQGLRGPLAGVPVLVKDNI
                     EVAGPLPTTAGSLALAANVTNRDAPLVARLRAAGAVIVGKANLSEWANIRSSDSISGW
                     SALGGQVRNPYALDRNPCGSSSGSGAAVAAGLVRMAVGTETDGSITCPAAVNGIVGFK
                     PTVGLVSRTHVIPISASQDTAGPMAASVREAALLLAAIAGSDPADPATRDADAHKEDY
                     AAALDAGALNGARIGVMRFASGFGTDEPFERALATLRAEGAVLVEIKGFDRGPIGANE
                     FTVLLTEFKAGLDDYLRSSPAPIPVRSLADVVAFNRANMGQEMPLFGQDIFDKAQATK
                     GLSDPAYKKARAVSLRLAGAQGIDRLLKEQRLDVLIGPTMPPAWKIDAVNGDQISGGG
                     AGKLAAVAGYPHLTVPMGLVKGLPVGLSFIGPKWSDTLMLRLGFAFEQARGPLPAPRF
                     LRSIEESPEIAPHLKPARR"
     gene            2672..3220
                     /locus_tag="VFU20_07525"
     CDS             2672..3220
                     /locus_tag="VFU20_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286342.1"
                     /translation="MMHLRNWQTATRKPFITAARTAAAVALGTSALIAAPALASPGSG
                     ITSTILATGDLDETVQFNHDRVKFQTKDPTDVRVQKLEFAAGAYTGWHHHPGIVIVTV
                     ASGLITTVQADCSTKTYGPGSGNGSVFVEGHDTPMEARSAAGGVVYATYVAPNASPPV
                     FRIEDPAQICSSVPSFQTPPRH"
     gene            complement(3312..3626)
                     /locus_tag="VFU20_07530"
     CDS             complement(3312..3626)
                     /locus_tag="VFU20_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEU5286343.1"
                     /translation="MAWFYLILGGLFEVGFTTALRFVDGFRNVPWTLAFLASVTISMG
                     LLELAARNIPMGTAYAVWGGIGALGTVVVGILWFDEPATLIRALLILTIVAAIAGLRL
                     TA"
     gene            complement(3639..4517)
                     /locus_tag="VFU20_07535"
     CDS             complement(3639..4517)
                     /locus_tag="VFU20_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5286344.1"
                     /translation="MTAFLVGILGIALFSGMDAVMKGLVLAIGTFATMFWRNLVGVGM
                     SGVLYLAQPSPWPSPSTMRLHLGRGILSTLMGFLFFWGLARVPLAQAIALAFIAPLIA
                     LYLAAVLLKETVGPRTVGASLIAFAGVLVIFVGQARADLGREATVGSLAILASAVCYA
                     LNIILMRRQAQVAGPTEITFFQSLIVTAILAALIPLVGIEVPAAVHWPWLLFAASLAI
                     TSYLLLSWAYARAEASYLAATEYTSFLWAALFGWLVFREELSPFTLAGAALIVAGCIL
                     AARKPADASPRLEAAA"
     gene            complement(4541..5674)
                     /gene="ispG"
                     /locus_tag="VFU20_07540"
                     /gene_synonym="gcpE"
     CDS             complement(4541..5674)
                     /gene="ispG"
                     /locus_tag="VFU20_07540"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HEU5286345.1"
                     /translation="MSSIRPWRTIERRKSRQIMVGNVAVGGDAPVSVQTMTNTPTADV
                     KATLDQIRRCEEAGADIVRVSCPDADSTAALRTIVREANVPIVADIHFHYKRALEAAD
                     AGAACLRINPGNIGSEERVREVIAAARYNGCAIRIGVNAGSLERDLLEKYAEPCPEAL
                     VESAMDHIRILEDHGFREYKVAAKASDLFLAVAAYQSLADQVDCPLHLGITEAGGLIG
                     GTVKSAIGMGALLWAGIGDTIRVSLSAEPEEEVRVGYELLKSLGIRNRGVRIVSCPSC
                     ARQGFDVIRTVEALEERLQHIRTPMSLSVLGCVVNGPGEARETDIGITGGGQGKHMVY
                     LSGVTDHHIRDEAMIDHIVALVEAKAAEIEAATERSETLDAAE"
     gene            complement(5723..5923)
                     /locus_tag="VFU20_07545"
     CDS             complement(5723..5923)
                     /locus_tag="VFU20_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HEU5286346.1"
                     /translation="MAGGDRKAYPLRVDPALWAAVERCAAADLRSVNSQVECLLREAL
                     KARGVKLKASEPRKRGRPPKAK"
     gene            complement(5928..6758)
                     /locus_tag="VFU20_07550"
     CDS             complement(5928..6758)
                     /locus_tag="VFU20_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU5286347.1"
                     /translation="MLLALLLAVAMQVYGVVDLARDNPAALPIAALVVGTLLLLLVSP
                     GFYMLQPNQATAITLFGDYRGTDRTTGLRWTWPWMIKKKLSVRANNFISERIKVNDQR
                     GNPIEMAAQIVWRVVDTAQALFDVDDYKQFVRVQVEAGIRTIGSRYPYDDFEHKDVTL
                     RGHLDEVGRELRREVIERLAVAGITVDECGFTHMAYAQEIAGAMLRRQQAQAVVAARQ
                     TLVAGAVGMVEMALEQLSEKNVVELDDERRAAMVSNLMVVLCGEREAQAVVNTGTLYT
                     "
     gene            6986..7933
                     /locus_tag="VFU20_07555"
     CDS             6986..7933
                     /locus_tag="VFU20_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HEU5286348.1"
                     /translation="MATVAHPQPPRDWKLVIHGGAGTIERAKITPEKDKAIRAGLDRA
                     LVTGSKVLESGGSALDAVEATVKVLEDDPNFNAGRGAVFTYEGVIELDASIMDGRTRA
                     AGAVASVTATRNPVSLARAVMEKSEHVFLAGDGADQFLREQGLPQVPNSYFATPNRRR
                     QLEEMKAGKVGALEIEYKYGTVGAVALDSRGHVAAATSTGGTTGKRWGRVGDSPIIGA
                     GTYADDRGCAVSATGSGEYFIRAAAAHEICARIRFRGEPPQAAADAVLKEIAELGGSG
                     GVIVLTPKGEQVYSFNTPGMYRGMADKSGRSVALYSDER"
     gene            complement(7915..9048)
                     /locus_tag="VFU20_07560"
     CDS             complement(7915..9048)
                     /locus_tag="VFU20_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5286349.1"
                     /translation="MADRETELIARLAPGVSQLDSGAWDRLAGGDPFVSHAFLAALEQ
                     SASVGPGTGWSPAPILVEDQAGALLAAAPAYLKSHSQGEYVFDHGWADAFERAGGRYY
                     PKLQVAVPFTPVPGPRLLGAWPQQLLAAAEAVTVQNGLSSAHVTFIDEAGAAEAERRG
                     WLIRHGIQYHWLNRGYRDFDDFLGRLTSRKRKAIRKERAAARAGLEFAALTGSAIGAA
                     EWNSMWAFYQDTGSRKWGRPYLTRSFFDRIGETMGDKLLLLLAYRGGEPIAGALNLIG
                     PDTLYGRYWGCVDEVPFLHFELCYYQAIEWACRHGLSSVQAGAQGEHKLARGYEPVIA
                     RSAHFIADPGFRSAVATFLDGERPAIAAEAEWERSALPYRSSE"
BASE COUNT         1459 a         3112 c         3242 g         1365 t
ORIGIN      
        1 ggcgtcgcgc gggtcgaggt cggggttttc gggcaattgc tgaagggcga tgtcgcgctc
       61 ggattcggtc agtgcgccat tgccttcggc gcgcaggttg agggttgttc ggctgtcccg
      121 gccgatcatc agccgggtga ggtcgccgag cccgccggca tagccgccct cggtcgcggc
      181 gcgggcctcc gcgcgggcgg cggtcgagcg gaaccggcgg cgcaggatga tgttcacgac
      241 gcgctggtcg gcccggtagc cgtatttgag ggcgacctcc tcgggcagga tctcgacccg
      301 ctggatcgcc tcggtcggga tatcgcgaag ctcgcgaaag ctcgagatcc gctggccgtt
      361 caggagcaat accggccgct cgccgccgcg gccccgggcg ctgccgatct gggggctgag
      421 cgcttccaag agctcgttga tgtcggtcgc cccggtcgcc cggacgtcgc gggcgtcgag
      481 cgtgttctcg ggcgggatgt cgccgatcac cgagccgcgc ggccgctggc cggtgacgac
      541 gatctccgct tcgtcttcgg cgaactcgtc gggcgcttgc gagccgttgg ccggcgcccc
      601 cggcggctcc tcggccggcg gctgcaccgt ctgcgcctga agcggcagtg cgaaggcggc
      661 gcccgcaagc agcgcgccgc gcgaaatcca tccggtcatt tcgtgggcgg tggcgcccgg
      721 caggtgaacc ttcgctgaca cggccgcgcc cttcgaccaa gcgtcgccag cgcccgacaa
      781 gcggcggggc tctggcgatg gaccaggaaa cttgccatcg tcggaaccag aaccggaagg
      841 tgcccatgac caacaagccc attgcattat ttgcggccat cgcctcgctt gccggttgcg
      901 ccgcggcttc ggccgaggac ccccgcttcg gcggtgtccc ggtggccacc gtcgccgctc
      961 cggttgcggc cccggccggg ccggcggagg cggcgacggc ggcggcgatc gaacgcatcg
     1021 ctcggatcga cccgcagctc ggctcggtgc tggcgctcga cccgacggcg atcgcccagg
     1081 cgcggcgtat cgacgcgcag ggtttgcgcg ggccgctcgc cggggtgccg gtgctggtca
     1141 aggacaatat cgaggtcgcg gggccgctgc cgacgaccgc gggcagcctg gcgctggccg
     1201 ccaacgtcac caaccgcgac gcgcctttgg tcgcgcggct gcgcgccgcg ggcgcggtga
     1261 tcgtcggcaa ggccaacctg agcgaatggg cgaacatccg ctcgtcggat tccatctccg
     1321 gctggagcgc tctcggcggg caggtccgca acccctatgc gctcgaccgc aacccgtgcg
     1381 gatcgtcgag cgggagcggc gccgcggtcg ccgccggcct ggtgcggatg gcggtcggaa
     1441 ccgagaccga cgggtcgatc acctgcccgg cggcggtcaa cgggatcgtc gggttcaagc
     1501 cgaccgtcgg cctggtcagc cggacccacg tgatcccgat cagcgccagc caggacaccg
     1561 cggggccgat ggccgcgagc gtccgcgagg cggcgctgct gctggccgcg atcgccggca
     1621 gcgacccggc cgaccccgcg acccgagacg ccgacgcgca caaggaggat tatgcggccg
     1681 cgctcgatgc gggggcattg aatggcgcgc ggatcggggt gatgcgcttc gcgtcgggct
     1741 ttggaaccga cgagccgttc gagcgcgcgc tcgcgacctt gcgggccgag ggcgccgtgc
     1801 tggtcgagat caaggggttc gaccgcggcc cgatcggcgc caacgagttc accgtccttc
     1861 tgaccgagtt caaggcgggc ctcgacgact atctgcggag cagtccggcg ccgatcccgg
     1921 tgcgcagcct cgccgacgtg gtcgctttca accgggcgaa catgggccag gaaatgcccc
     1981 tgttcggaca ggacattttc gacaaggccc aggcgaccaa ggggctgtcg gacccggcgt
     2041 acaagaaagc ccgtgcagtg agcctcaggc tggcgggcgc gcagggcatc gaccggctgt
     2101 tgaaggagca gaggctcgac gtcctgatcg ggccgaccat gccgccggcg tggaagatcg
     2161 acgcggtcaa cggcgaccag atttcgggcg gcggggcggg caagctggcc gcggtcgccg
     2221 gctacccgca cctcacggtg ccgatggggc tggtcaaggg cctgcctgtg ggcctcagct
     2281 tcatcgggcc caaatggtcg gacacgctga tgcttcggct cggttttgcg ttcgagcagg
     2341 cgcgcgggcc gctgccggcg cccagattcc tgcgctcgat cgaggagagc cccgagatcg
     2401 cgccgcacct caagccggcg cgcaggtaac atcccgtgat tggacgatga gctgcgcgtt
     2461 ttctgctttc gccatgccgt ctatctcatg tggataactt ctgttaatgc cgtcgctgcc
     2521 tatcgcgacc gagtcgcaac cgcggaccga gaccaagagc ctcattcgct cttccggcgc
     2581 cttcacgaga cagcatcaac gaccgggcca agcgactccg ctgtggagcc gcttggtggc
     2641 atgggcgctt ttactaacaa ggggctgaaa gatgatgcat cttcgtaact ggcagaccgc
     2701 aactcggaag ccgttcatta cagctgcgcg aacggcggca gccgtagcgc tcggaacatc
     2761 cgcgttgatc gcagcgccgg cactagcctc ccccggatcc ggcatcacct ctaccatcct
     2821 cgcgacgggc gacctggatg aaactgtcca gttcaatcac gatcgagtga aattccagac
     2881 caaggacccg accgacgtcc gcgtacagaa actcgagttt gcagccggcg cctataccgg
     2941 ctggcaccat cacccgggca tcgtcatcgt gacggtggcg tcgggcctga tcaccacggt
     3001 tcaagccgac tgttcgacca agacctacgg cccgggctca ggaaacggat cggtgttcgt
     3061 cgaaggtcat gacacaccga tggaagctcg cagcgcggcc ggcggtgtcg tctatgcgac
     3121 ctatgtggcg cccaatgctt ccccgccggt attccgcatc gaagatccgg cgcagatctg
     3181 cagctcggtt ccaagcttcc aaacgcctcc gagacactaa tctcgagccc agatgcaggc
     3241 cccgcctcag tgctcggggc ctgcacgggc gcggttgagc cggcccggga aggtcactgc
     3301 caacgatcgg cctaggcggt gagccgcagg cccgcgatcg cggcgacaat ggtcaggatc
     3361 agcagcgcgc ggatgagggt cgcgggctcg tcgaaccaga ggatcccgac gaccaccgtc
     3421 cccagcgcgc cgatgccgcc ccagaccgcg taggcggtgc ccatcgggat attgcgcgcg
     3481 gcgagctcga gcaggcccat gctgatggtc accgaggcga ggaaggcgag cgtccacggc
     3541 acgttccgga agccgtcgac gaagcgcagc gcggtggtga agccgacttc gaacaggccg
     3601 ccgagaatga ggtagaacca ggccatgctt gcctccgttc aggcggccgc ctcgagccgc
     3661 gggctggcgt cggcgggctt gcgggcggcg aggatgcagc cggcgacgat cagcgccgcc
     3721 ccggccaggg tgaagggcga cagctcctcg cggaacacca accagccgaa cagcgcggcc
     3781 cacaggaacg aggtgtattc ggtcgccgcg agatagctcg cctcggctcg ggcataggcc
     3841 caggacagca gcaagtaaga ggtgatcgcg aggctcgccg cgaacagcag ccacggccag
     3901 tggaccgccg ccggcacctc gatcccgacc agcgggatca gtgcggcgag gatcgcggtg
     3961 acgatcaggc tctggaagaa ggtgatctcg gtcgggccgg cgacctgggc ctggcggcgc
     4021 atcaggatga tgttgagcgc gtagcagacc gcgctggcga ggatggcgag gctgccgacg
     4081 gtcgcctcgc ggccgaggtc ggcccgggcc tggccgacaa agatcaccag caccccggca
     4141 aacgcgatca gcgacgcccc gacggtgcgc gggccgacgg tttccttgag cagcacggcg
     4201 gcgaggtaga gcgcgatcag cggcgcgatg aaggcgagcg cgatcgcctg ggcgagcggc
     4261 acccgcgcca ggccccagaa gaacaggaag cccatcaggg tcgagaggat cccgcggccg
     4321 aggtgcagcc gcatggttga aggcgacggc cacggcgagg gctgggcgag gtagagcacg
     4381 cccgacatgc cgaccccgac gaggttgcgc cagaacatgg tcgcgaaggt gccgatcgcc
     4441 agcaccagcc ccttcatcac cgcatccatg cccgagaaca gagcgatgcc gagaatcccg
     4501 acgaggaaag cagtcatcgg cgggtggtgg cccggcggcg tcactccgcg gcgtcgagcg
     4561 tttcggagcg ctcggttgcg gcctcgatct cggcggcctt ggcctcgacc agggcgacga
     4621 tgtggtcgat catcgcctcg tcgcggatgt ggtggtcggt gacgcccgac agatagacca
     4681 tgtgcttgcc ctggccgccg ccggtgattc cgatgtcggt ctcgcgcgcc tcgccggggc
     4741 cgttgacgac gcagcccaat accgacagcg acatcggggt gcggatatgc tgcagccgct
     4801 cctccagcgc ctcgacggtg cggatgacgt cgaagccctg gcgggcgcag cttgggcagg
     4861 agacgatccg caccccgcgg ttgcggatcc cgaggctctt cagaagctcg tagccgaccc
     4921 gcacttcctc ctcgggctcg gccgacagcg acactcggat ggtgtcgccg atccccgccc
     4981 acagcaaggc gcccatgccg atcgccgact tgacggtgcc gccgatcagg ccgccggcct
     5041 cggtgatgcc gaggtgcagc gggcaatcga cctggtcggc caggctctgg taggcggcga
     5101 cggcgaggaa caggtccgac gccttcgccg ccaccttgta ttcgcgaaag ccgtggtcct
     5161 cgaggatccg gatatggtcc atcgcgcttt cgaccaaagc ctcggggcac ggctcggcat
     5221 atttctcgag caggtcgcgt tcgagactgc cggcgttgac cccgatgcgg atcgcgcagc
     5281 cgttgtagcg cgcggcggca atgacctcgc ggacgcgttc ctccgagccg atgttgccgg
     5341 ggttgatcct gaggcaggcg gcgccggcgt cggccgcctc gagagcgcgc ttgtagtgga
     5401 aatggatgtc ggcgacgatc ggcacattgg cctcgcggac gatggtccgg agcgccgcgg
     5461 tgctgtcggc gtcggggcac gacacccgga cgatgtcggc gcccgcctcc tcgcagcggc
     5521 ggatctggtc gagggtcgcc ttgacgtccg cggtcggggt gttggtcatg gtctggacgc
     5581 tgaccggcgc gtcgccgccg acggcgacgt tgccgaccat gatctggcgc gacttgcgcc
     5641 gctcgatggt gcgccagggg cggatggagg acatgacgcg ggcgatatag gcgctcggcg
     5701 gcgcggggca aaggcgcggg cgctacttcg ccttcggcgg ccggccgcgc ttcctcggct
     5761 ccgatgcctt gagcttgacc ccgcgcgcct tcaaggcctc gcgcagcagg cactcgacct
     5821 ggctgttgac gctccggagg tcggccgcgg cgcagcgctc gaccgcggcc cacagcgccg
     5881 gatcgaccct tagcgggtac gccttgcggt cgccgccggc cacgagccta ggtgtagagc
     5941 gtgccggtgt tgacgacggc ctgcgcttcg cgctcgccgc acagcacgac catcaggttc
     6001 gacaccatcg ccgcgcggcg ctcatcgtcc agctcgacga cattcttctc cgacaattgc
     6061 tcgagcgcca tctcgaccat gccgaccgcg cccgcgacca gcgtctggcg ggccgcgacc
     6121 accgcctgcg cctgttgacg gcggagcatc gccccggcga tctcctgggc ataggccatg
     6181 tgcgtgaagc cgcattcgtc gaccgtgatt ccggcgaccg cgaggcgctc gatcacttcc
     6241 ctcctgagct cgcgcccgac ttcgtcgagg tggccgcgca gggtcacgtc cttgtgctcg
     6301 aaatcgtcgt acggatagcg cgagccgatg gtgcggatcc ccgcctcgac ctggacccgg
     6361 acgaattgct tgtagtcgtc gacgtcgaac agggcctggg cggtgtcgac cacgcgccag
     6421 acgatctgag ccgccatctc gatcgggttg ccgcgctggt cgttgacctt gatccgctcg
     6481 gaaatgaaat tgttggcccg gaccgagagt ttcttcttga tcatccacgg ccaggtccag
     6541 cgcagcccgg tggtgcggtc ggtgccgcga tagtcgccga acaaagtgat cgccgtcgcc
     6601 tggttgggct gcagcatgta gaagccgggc gagaccagca gcagcaagag ggtgccgacg
     6661 acgagcgccg cgatcggcag cgccgcggga ttgtccctcg ccagatcgac cacgccgtag
     6721 acctgcatcg cgaccgccag cagcagagcc agcagcatga tgtagccgtt cgaggtcgag
     6781 gccgagcgct cgcggctcga cgtcagcgca accacgtcac ccttcgcacc ttttggatcc
     6841 tttgccacag catcctccct taaattgata tcataataat atcatcacac ctcgcccgtc
     6901 aactggaatc ggatggacgg cgcggctatg cacccgccaa cggagtgccg ccatgcgctt
     6961 gctgctgatc cttggagtgt cgatgatggc cactgtcgcc cacccccagc cgccacggga
     7021 ctggaagctc gtcatccacg gcggcgccgg gacgatcgag cgggccaaga tcacgccgga
     7081 gaaggacaag gcgatccgcg ccggcctcga tcgcgcgctg gtgaccgggt cgaaagtgct
     7141 cgagtccggc ggcagcgcgc tcgatgcggt cgaggcgacg gtcaaggtgc tggaggacga
     7201 tcccaacttc aacgccggcc gcggcgcggt cttcacctac gaaggcgtga tcgagctcga
     7261 cgcgtcgatc atggacggcc ggacccgcgc ggccggagcg gtcgcgagcg tcaccgccac
     7321 ccggaacccg gtcagcctcg cccgggcggt catggaaaag agcgagcacg tgttcctggc
     7381 cggcgacggc gccgaccagt tcttgcgcga gcagggattg ccgcaggtcc cgaacagcta
     7441 tttcgcaacc cccaaccgcc gccgccagct cgaggagatg aaggccggca aggtcggcgc
     7501 tctcgaaatc gagtataaat acggcacggt cggagccgtg gcgctcgact cccgcggcca
     7561 tgtcgccgcc gcgacctcga ccggaggcac gaccggcaag cgctggggcc gggtcggcga
     7621 cagtccgatc atcggcgccg gcacctatgc cgatgaccgc ggctgcgcgg tctcggcgac
     7681 cgggtccggg gaatatttca tccgcgccgc ggcggcgcac gaaatctgcg ctcggatccg
     7741 cttccgcggc gaaccgcccc aggcggcggc cgacgccgtg ctcaaggaga tcgccgagct
     7801 cggcgggagc ggcggagtga tcgtcctgac cccgaagggc gagcaggtct attcgttcaa
     7861 cacgcccgga atgtaccgcg gcatggccga caaaagcggg cgctcggtcg cgctctactc
     7921 cgacgagcgg tagggcagcg ccgagcgctc ccattcggct tcggcggcga tggccggccg
     7981 ctcgccgtcg aggaaggtcg cgaccgcgct gcggaagccc gggtcggcga tgaaatgggc
     8041 cgagcgggca atcaccggct cgtagccgcg ggcgagcttg tgctcgccct gcgcgccggc
     8101 ctggaccgag gacaggccat gccggcacgc ccattcgatc gcctgataat agcacagctc
     8161 gaaatggagg aacggcacct cgtcgacgca tccccaatag cggccgtaca gcgtgtcggg
     8221 gccgatcagg ttgagggcgc cggcgatggg ctcgccgccg cgataggcga ggaggagcaa
     8281 cagcttgtcg cccatggtct cgccgattcg gtcgaagaac gagcgggtca ggtagggccg
     8341 gccccatttg cggctgccgg tgtcctggta gaaggcccac atcgaattcc actcggcggc
     8401 gccgatcgcg ctgccggtca gcgccgcgaa ctcgagcccg gcgcgggcgg cggcgcgctc
     8461 cttgcggatc gccttgcgct tgcggctggt cagccggcca aggaaatcgt cgaagtcgcg
     8521 atagccgcgg ttgagccagt ggtactggat gccgtggcgg atcagccagc cgcgccgctc
     8581 ggcctcggcc gcccccgcct cgtcgatgaa ggtgacgtgg gccgacgaca ggccgttctg
     8641 gacggtcacc gcctcggccg cggcgagcaa ttgctgcggc caggccccaa gcagccgcgg
     8701 tcctgggacc ggggtgaacg gcaccgcgac ctggagcttg gggtaatagc gcccgccggc
     8761 gcgctcgaag gcgtcggccc agccgtggtc gaacacatat tcgccctggc tgtggctctt
     8821 cagataggcg ggcgcggcgg cgagcagggc cccggcctga tcctcgacca ggatcggcgc
     8881 gggcgaccag ccggtgccgg ggccgacgct ggccgattgc tcgagcgcgg cgaggaacgc
     8941 atggctgacg aacgggtcgc cgcccgcgag ccggtcccag gcgccagagt cgagttggga
     9001 aacccccggc gcgaggcgcg cgatcagctc ggtttcacgg tcggccatcg gcttcccaga
     9061 tgagcgcgtc cgagtgaacc cgcgcttgct cgcgctcggc gggcgtgcgg atcgtccacg
     9121 cgtagaccgg tatccgccgc cgcgcccggg cgacccagcg gcgctcgagc gcggcccg
//
LOCUS       DASTZO010000270         2259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_409153,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000270 DASTZO010000000
VERSION     DASTZO010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2259
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409153"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFU20_07565"
     CDS             <1..189
                     /locus_tag="VFU20_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286350.1"
                     /translation="ADEAGNQGESAAEPAEGAVEDAPATADSPEPAEAAAEEAAPADE
                     PVENAEAPAEGEAEEKAE"
     gene            192..692
                     /gene="rimM"
                     /locus_tag="VFU20_07570"
     CDS             192..692
                     /gene="rimM"
                     /locus_tag="VFU20_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048866.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEU5286351.1"
                     /translation="MATPATEGRRIPLAAVAGAHGIKGEVRLKLFSDSAESLARHATL
                     FVGGAERRLLGVRAAGKGAVARFEAIADRTAAESLRGALVEVDRASLPPLAEGEYYHA
                     DLVGLPCVDREGRALGTVAAVENFGAGDLLDVADPDGRRSLIPFKPGIADLADGRVVL
                     DPEFLA"
     gene            complement(694..1608)
                     /locus_tag="VFU20_07575"
     CDS             complement(694..1608)
                     /locus_tag="VFU20_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5286352.1"
                     /translation="MDKPLDCLIVGGGPAGLTAAIYLARFHLDILLVDAGKSRAAMIP
                     CTHNHAGFPDGISGKELIGRMKEQAQLYGARIVEDHVTRLDCEGELFTAEWGSGSVSA
                     KTVLLATGVANRRPPMDEGLHDEALARGLIRYCPICDGYEVTDKKVGVIGSDSHGVAE
                     ALFLRGFTRDVTLIAPHAELDLPAEDQAKLKAAGIACVDGPAEAVAALDKCIVVDTAQ
                     GHHTFDSVYPALGSDTHVQLAEMVGARLGEDGCIGVDARQRTSVPGVYAAGDVVIGLD
                     QISHAMGEGGVAATTIRNDLARDRPLLR"
     gene            1692..1985
                     /locus_tag="VFU20_07580"
     CDS             1692..1985
                     /locus_tag="VFU20_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286353.1"
                     /translation="MVAAVAASTFVVAWFLGRMTAWPERRVAMISAIPIPGVGIALAL
                     VMIVSVSTASDEDCGVDACGMMAAAAFTTIVLAVIAFAINYFAGRSACRLARK"
     gene            1982..>2259
                     /locus_tag="VFU20_07585"
     CDS             1982..>2259
                     /locus_tag="VFU20_07585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU5286354.1"
                     /translation="MTFTATVLTLYPEMFPGPLGLSIAGRALADGLWSLDTRNIRDFA
                     TDKHKSVDDTPAGGGAGMVLRPDVLAAALDSVAAEGKGTRPMIALTPRG"
BASE COUNT          323 a          794 c          781 g          361 t
ORIGIN      
        1 gccgacgagg ccggcaacca gggcgaatcc gccgccgaac cggccgaagg cgcggtggag
       61 gacgctccgg cgaccgccga ctcgcccgag ccggccgagg ccgctgccga agaggctgcg
      121 cccgctgacg agcctgtcga gaacgccgag gcgccggccg agggtgaagc cgaagagaag
      181 gccgaataat catggcgacg ccggcgacgg aggggcggcg catcccgctc gccgccgtcg
      241 ccggcgcgca cggcatcaag ggcgaggtcc gactgaagct gttctccgac agcgccgaga
      301 gcctcgcccg ccacgcgacc ctgttcgtcg gcggcgccga gcgccggctg ctcggggtgc
      361 gggccgccgg caagggcgcg gtcgcccgct tcgaggccat cgccgaccgc accgccgcgg
      421 aaagcctgcg cggcgcgctg gtcgaggtcg accgggcgtc gctgcccccg ctcgccgagg
      481 gcgaatatta ccatgccgac ctggtcgggc tgccttgcgt cgaccgcgag ggccgagcgc
      541 tcggcaccgt cgccgcggtc gagaatttcg gtgccggcga tctgctcgac gtcgcggacc
      601 ccgacggccg ccgctcgctc attcccttca agcccgggat cgccgacctc gccgacggcc
      661 gggtcgtgct cgacccggag ttcctcgcct gatctaccgc aggagcggcc gatccctggc
      721 gagatcgttg cggatggtgg tcgcggcgac cccgccttcg cccatcgcgt ggctgatctg
      781 gtcgagcccg atgaccacgt cgccggccgc atagaccccc gggacgctgg tccgctggcg
      841 cgcatcgacg ccgatgcagc cgtcctcgcc gagccgggcg ccgaccatct cggcgagctg
      901 gacatgggtg tccgacccga gcgcggggta gacgctgtcg aacgtgtgat gcccttgcgc
      961 ggtgtcgacg acgatgcact tgtcgagcgc cgcgaccgcc tcggccgggc cgtcgacgca
     1021 cgcgattccg gcggccttga gcttcgcctg gtcctcggcc ggcaggtcca gctcggcgtg
     1081 gggggcgatc agggttacgt cgcgggtgaa gccgcgcagg aacagcgcct ccgcgacccc
     1141 gtgcgaatcg ctgccgatca ccccgacctt cttgtcggtg acctcgtagc cgtcgcagat
     1201 cgggcaatag cggatgaggc cgcgggccag cgcctcgtcg tgcagcccct cgtccatcgg
     1261 cgggcggcgg ttggcgaccc cggtggccag cagcaccgtc ttggcgctca ccgagcccga
     1321 cccccattcg gccgtgaaca gctcgccctc gcagtcgagc cgggtcacgt gatcctcgac
     1381 gatccgcgcc ccataaagct gcgcctgctc cttcatccgc ccgatcagct ccttgccgct
     1441 gatgccgtcc gggaagccgg cgtggttgtg ggtgcacggg atcatcgcgg ctcggctctt
     1501 gcccgcgtcg accagcagga tgtcgaggtg gaaccgggcg aggtagatgg cggcggtcag
     1561 cccggccggg ccgccgccga cgatcaggca gtcgaggggt ttatccatca cggcagaaac
     1621 ctgtgcttcc gtgcttgttc cgcattcgct aggtcgaagg catgtttccg ataggactaa
     1681 ccgaactcct gatggtcgct gccgtggctg cgtcgacctt tgtggttgct tggtttctgg
     1741 gacgaatgac agcttggcca gaacgtcgcg tcgcaatgat ttcagccata ccgattcccg
     1801 gagttggaat tgcactcgca cttgtgatga tcgtgagcgt atcaaccgct tctgatgagg
     1861 attgtggtgt agacgcctgc ggcatgatgg cggccgctgc attcactacc atcgtactcg
     1921 ctgtcatcgc cttcgcgatc aattactttg cggggcgttc ggcgtgccgg cttgcgagga
     1981 aatgactttt accgcgaccg tgctgacgct ttaccccgag atgttcccgg ggccgctcgg
     2041 cctcagcatc gccggacggg cgctcgccga cgggctctgg tcgctcgaca cccgcaacat
     2101 ccgcgacttc gcgaccgaca agcacaagag cgtcgacgac actccggccg gcggcggggc
     2161 cgggatggtg cttcgccccg acgtgctcgc ggcggcgctc gacagcgtcg ccgccgaggg
     2221 caagggcacc cggccgatga tcgccctgac cccgcgcgg
//
LOCUS       DASTZO010000271         4455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1015122,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000271 DASTZO010000000
VERSION     DASTZO010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4455
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1015122"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..1678
                     /locus_tag="VFU20_07590"
     CDS             29..1678
                     /locus_tag="VFU20_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003043526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEU5286355.1"
                     /translation="MKAMAGWSEGDWIAALRALALFAFLAIGAVLATRRSQEVIGHPR
                     GLFVLFYAEMWERFSYYGMRALLVLYLTKFWLFADGKANLIYGAYGSLVYITPVLGGY
                     VADRWLGQRRAVLFGGILLMCGHFLMAVEGVGGQNDPTINVFWTALAFIIVGSGFLKA
                     NISVMVGQLYKLTDTRRDGAYTIFYMGINLGAAVGTILVGYLGETVGWAYGFGLAGIG
                     MLAGLVVFVLGRPALRGAGEAPRALAKNTEWTLYGVGLAAVAVIWALIQYQDIIQTLL
                     IVSGIGLLGYVLYEAFKLDREPRQRIFAILFLIALNPVFWGLFEQAGGSINLYTDRYV
                     DRAGVPASLFQSINPIYILLLAPIFAGLWVTLGRRGMEPSAPAKFALALLQVGAGFLV
                     FVWGANAFPVGDEAKTAVLFVFLLYLLHTTGELCLSPVGLSAMNRLAPPFMASLIMGA
                     WFYMTAVGNFVAGKIGEATGGHDGEMTKEGTLAVYNTIGWWTIGIGVVVLMLSPIVKR
                     WMHLDTLRDDDLAGQAELAEPAAPGMKPQRETRPDPDPARI"
     gene            1741..3072
                     /locus_tag="VFU20_07595"
     CDS             1741..3072
                     /locus_tag="VFU20_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5286356.1"
                     /translation="MPKPKPAPPIRINEDPFPSTYVRYPGVPTVIRNATVFDGEGGRI
                     DGGTVVLADGVVQSVGGAGLAAPAGAVEIDGTGKYVTPGIIDVHSHLGDYPSPAVSSH
                     SDGNEATDPARPEVWAEHSVWPQDPGFSRALANGGITALQVLPGSANLFGGRSVTLKN
                     VYARTVQGMKFPGAPYGLKMACGENPKRVYGSKGNIPQTRMGNIALTRATWLKAQAYK
                     RKWDAYERKGGTMPERDLAMDTLRGVLAGKILIHNHCYRADEMAIMIDVAREMGYRIG
                     TFHHAVESYKVADLLAANQICSAMWADWYGFKMESYDAIKENIPFVHNSGACAIVHSD
                     DPNGIQRLNQEAAKALADGRRAGINISEEVAWTWLSANPARALGIFDKTGSLKPGKMA
                     DVVLWNANPFSVYARPERVWIDGALMYDANDPKRRPVSDFELGQPGEGDVK"
     gene            3162..>4455
                     /locus_tag="VFU20_07600"
     CDS             3162..>4455
                     /locus_tag="VFU20_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5286357.1"
                     /translation="MALISTPATAQTIAITGGTVATGDGSEPVSGTVVIRNGRIVAAG
                     PAVGVPAGATIVDATGKWVTPGIVAGFSRLGLAEVDLSVEGSVDTQADNGPFSAAIDV
                     APAINPLASTIAVNRADGVTRAIVAPGAAKSIFAGQGAVIDTGADMDPITAARRFQFV
                     ELGETGASKAGGSRASAHVLFRNALREAAELRRFAVPIASGAVAEPDERLRPEIRDPN
                     ESRIIGPDARRSEDVLLTRFDAAALVPVLQGRQYLLVHAERASDILQVIRLKREFPAL
                     KLVLVGASEGWTVADRIAASGIPVIASPVKDLPASFEQLAATQSNVGRMGAAGVKVSI
                     GMIDDDDTRNIFYQRQYAGNLVALRNVPGATGVSWGEALAMITSRSAEAVGLGREIGS
                     LLPGRRGDVVIWSGDPLEGASAAEMVFIDGVQQPLANHQ"
BASE COUNT          694 a         1532 c         1523 g          706 t
ORIGIN      
        1 cggatcattc gcggaggaac gggcggcaat gaaggcaatg gcagggtggt ccgaggggga
       61 ctggatagcc gcactcagag cgcttgcgct gttcgccttc ctcgcgatcg gcgctgtgct
      121 cgccacccgc cgcagccagg aggtgatcgg ccatccccgc ggcctgttcg tgctgttcta
      181 tgccgaaatg tgggagcgct tctcctacta cggcatgcgc gccctgctgg tgctctacct
      241 caccaagttc tggctcttcg ccgacggcaa ggccaacctg atctacggcg cctacggcag
      301 tctcgtctat attacccccg tcctcggcgg ttacgtggcc gatcgctggc tcggccagcg
      361 ccgggcggtg ctgttcggcg gcatattgct gatgtgcggc cacttcctga tggcggtcga
      421 gggagtcggc ggccagaacg acccgaccat caacgtcttc tggaccgcgc tcgcgttcat
      481 catcgtcggc tcgggcttcc ttaaggccaa catctcggtg atggtcgggc agctctacaa
      541 gctgaccgat acccgccgcg acggcgccta caccatcttc tacatgggca ttaatctcgg
      601 cgccgcggtc ggcaccatcc tggtcggcta tctcggcgag acggtcggct gggcctacgg
      661 cttcggcctt gccgggatcg gcatgctggc cggcctcgtc gtgttcgtgc tcggccgccc
      721 ggcgctgcgc ggagcgggcg aagcgcccag ggccctggcg aagaacaccg aatggaccct
      781 gtacggggtc gggctcgccg ccgtcgccgt gatctgggcg ctgatccagt accaggacat
      841 catccagacc ctgctgatcg tctccggcat cgggctgctc ggctatgtgc tctacgaagc
      901 cttcaagctc gaccgggagc cgcgccagcg catcttcgcg atcctgttcc tgatcgcctt
      961 gaacccggtg ttctggggcc tgttcgagca ggcgggcggg agcatcaacc tctacaccga
     1021 ccgctacgtc gaccgcgccg gagtgcccgc gtcgctgttc cagtcgatca acccgattta
     1081 catcctgctt ctggcgccga tcttcgccgg cctgtgggtg acgctcgggc ggcgcgggat
     1141 ggagccctcg gccccggcca agttcgcgct cgccctgctc caggtcggcg ccggcttcct
     1201 ggtgttcgtc tggggcgcca atgccttccc ggtcggggac gaagccaaga cggcggtgct
     1261 gttcgtgttc ctgctctatc tgctgcacac gaccggagaa ttgtgcctgt cgccggtggg
     1321 cctgagcgcg atgaaccggc ttgccccccc cttcatggcg agcctgatca tgggcgcctg
     1381 gttctacatg accgcggtcg gcaatttcgt cgccggcaag atcggcgagg cgaccggcgg
     1441 ccacgacggc gaaatgacca aggaagggac gctcgcggtc tacaacacca tcggctggtg
     1501 gacgatcggg atcggcgtcg tggtgctcat gctgtcgccg atcgtgaagc ggtggatgca
     1561 cctcgacacg ctcagggacg acgatctggc ggggcaggcg gagctcgccg agcccgcggc
     1621 tccgggcatg aagccgcaac gcgagacccg ccccgacccg gacccggcgc gcatctgatg
     1681 cggcgcaggc tttggggggc agcgctgatc gcgttcgctc tgcccggttg cgcccagctg
     1741 atgcccaagc cgaaaccggc gcctccgatc cggatcaacg aggacccgtt cccgtcgacc
     1801 tacgtccgct atcccggcgt cccgacggtg atccgcaacg ccaccgtgtt cgacggcgag
     1861 ggcgggcgga tcgacggcgg cacggtggtg cttgccgacg gcgtcgtgca gtcggtcggc
     1921 ggcgccgggc tggcggcgcc cgcgggggcg gtcgagatcg acggcaccgg caaatatgtg
     1981 actcccggga tcatcgacgt ccacagccac ctcggcgatt atccgtcgcc ggcggtttcg
     2041 tcccattcgg acggcaacga ggcgaccgac ccggcgcggc ccgaggtatg ggccgagcac
     2101 agcgtctggc cgcaggaccc ggggttcagc cgcgcgctcg ccaacggcgg aatcaccgcc
     2161 ctgcaggtgc tgcccggctc ggcgaacctg ttcggcggcc gctcggtcac cctcaagaac
     2221 gtctatgcgc gcaccgtcca ggggatgaaa ttccccggcg caccgtacgg gctcaagatg
     2281 gcgtgcggag agaacccgaa gcgggtctat ggctcgaagg gcaacatccc gcagacccgg
     2341 atgggcaata tcgcgctgac ccgggcgacc tggctcaagg cgcaggcgta caagcgcaag
     2401 tgggacgcct acgagcgcaa gggcggcacc atgcccgagc gcgacctggc gatggacacg
     2461 ctgcgcggcg tgctggccgg caagatcctg atacacaatc attgctatcg cgccgacgag
     2521 atggcgatca tgatcgatgt cgcccgcgag atgggctacc ggatcggcac cttccatcac
     2581 gcggtcgaga gctacaaggt cgccgacctg ctggccgcga accaaatctg ctcggcaatg
     2641 tgggccgact ggtacggctt caagatggaa agctatgacg cgatcaagga gaacatcccg
     2701 ttcgtccaca attcgggcgc ctgcgcgatc gtccattccg acgatcccaa cggcatccag
     2761 aggctgaacc aggaggccgc caaggcgctc gccgacggcc gccgggccgg gatcaacatc
     2821 agcgaggagg tcgcctggac ctggctcagc gcgaacccgg ccagggcgct cgggatcttc
     2881 gacaagaccg gcagcctcaa gcccgggaag atggccgacg tcgtgctgtg gaacgccaat
     2941 ccgttcagcg tctacgcccg gcccgaacgg gtgtggatcg acggcgccct gatgtacgac
     3001 gccaacgatc ccaagcgccg cccggtcagc gatttcgagc tcggccagcc gggcgaaggg
     3061 gacgtcaaat gagcaaaccc tcaccctccc accgctgcgc ggcgggtccc tccctctccc
     3121 gcgcgcggga gagggctggg gtgagggtat ttcttcttgc gttggcgcta atctcaactc
     3181 ccgccactgc tcagaccatc gcgatcaccg gcgggacggt cgccacgggc gacggcagcg
     3241 agccggtttc cggcaccgtc gtcatccgca acgggcggat cgtcgccgcc gggcccgcgg
     3301 tcggcgtccc ggccggagcg acgatcgtcg atgcgaccgg caaatgggtg accccgggca
     3361 tcgtcgccgg cttctcgcgc ctcggcctcg ccgaagtcga tctcagcgtc gagggaagcg
     3421 tcgacaccca ggccgacaac gggccgttca gcgccgcgat cgacgtcgct ccggcgatta
     3481 acccgctcgc ctcgaccatc gccgtcaacc gcgccgacgg ggtgacccgg gccatcgtcg
     3541 cgccgggggc ggccaagagc atcttcgccg gccagggcgc ggtcatcgac accggcgccg
     3601 acatggaccc gatcacggcc gcgcgccgct tccagttcgt cgagctcggc gagaccgggg
     3661 cgtcaaaggc cggcgggtcg cgggcgtcgg cccacgtgct gttccgcaac gcgctgcgcg
     3721 aggccgcgga gctgcgccgc ttcgcggtgc cgatcgcgtc gggcgccgtt gcggagcccg
     3781 acgagcgcct gcggccggaa atccgcgacc ccaatgaatc gcgcatcatc ggccccgacg
     3841 cgcggcgcag cgaggacgtg cttctgaccc ggttcgacgc cgccgccctg gtcccggtgc
     3901 tccaggggcg ccagtatctg ctggtccacg ccgagcgtgc gagcgacatc ttgcaggtga
     3961 tccgcctcaa gcgcgagttc ccggcgctca agctggtgct ggtcggggcg agcgagggct
     4021 ggacggtcgc cgaccggatc gccgcgtcgg ggatcccggt gatcgcctcg ccggtcaagg
     4081 atctgccggc gtcgttcgag cagctcgccg cgacccagtc caacgtcggc cggatgggcg
     4141 ccgcgggggt caaggtgtcg atcgggatga tcgacgacga cgacacgcgc aacatcttct
     4201 accagcggca atatgctggc aacctcgtcg cgctgcgcaa cgtccccggg gcgaccggag
     4261 tcagctgggg cgaggcgctg gcgatgatca cctcgcggtc ggccgaggcg gtcggcctcg
     4321 ggcgcgagat cggcagcctg ctgccgggtc ggcgcggcga cgtggtgatc tggtcgggcg
     4381 acccgctcga gggagccagc gccgcggaaa tggtgttcat cgacggagtc cagcaaccat
     4441 tggccaacca ccaga
//
LOCUS       DASTZO010000272         7821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_963116,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000272 DASTZO010000000
VERSION     DASTZO010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7821
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963116"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VFU20_07605"
     CDS             <1..976
                     /locus_tag="VFU20_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HEU5286358.1"
                     /translation="EATALVRDLVDTPAGDLGPAELEAAVRDHVAAVGAEVRVTSGDA
                     LAEGYPLVAAVGAAASRERAPRLVEAEWGDPGHPRVAIVGKGVCFDSGGLDLKTASGM
                     RLMKKDMGGAAHALALFVLAAAGRLPVRLHLLIPAVENAVSAAALRPGDIVRSRKGLQ
                     VEIDNTDAEGRLILADALARAAEGDGEKSRPELILDFATLTGAARVALGPDLPALFAN
                     RDELLDGFEAAAREVEDPLWRMPLWDGYDDMLKSDVADIANATGSQLAGCITAALFLR
                     RFVPGETQWAHLDTFAWRPKAKPGRPKGGDALGLRAAFALLCERYPAR"
     gene            1099..1479
                     /locus_tag="VFU20_07610"
     CDS             1099..1479
                     /locus_tag="VFU20_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011950949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HEU5286359.1"
                     /translation="MSEVSLTGWMKALIAYVRSGEPDLTNRQMALLMLVYLTPGPHTV
                     RGLAKILGVSKPVVTRALNTLGALGYLRRERDQDDRRNVFVVRTSNGANFLEGFKRNL
                     REGSEGAPAAHRPIIGTAAFAHQR"
     gene            1610..1783
                     /locus_tag="VFU20_07615"
     CDS             1610..1783
                     /locus_tag="VFU20_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286360.1"
                     /translation="MSRAGTLRAGPCDEAEALLELNPGDPFAMLDNSLGWAWGYAGKE
                     RRVGYLRSEAVEA"
     gene            1863..4139
                     /locus_tag="VFU20_07620"
     CDS             1863..4139
                     /locus_tag="VFU20_07620"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEU5286361.1"
                     /translation="MSRVFVSYSRQDAPRAKALAKALQAAGHEVWWDSHIRGGSRFGA
                     EIAEALKNAEVVVVLWSRSSIDSAWVNDEAAEGRDTGRLVPILIDDCKPPLGFRQFQA
                     IDLRDWTGRRTLPHHVLDAISAKTADGPLPAPRPGAEERRGWQLGRRPLIALLALAVL
                     VAGAWAYFNRTSAGGAEVPTLAVLPFADLSPNRDKGYFAEGVAESILSLLGREPGIRV
                     IGRTSASLFKDRGGDLAAMRKSLGVTHVLEGSARTAGDDLRLSVRLIDAADGTQLWAQ
                     DYQRNLRNVFAVQDEIGKSVATELLGTLSTAGRTRAPITRADAYDLYLATRATMRNRR
                     EPVLRKALGMAQRVIAADPDYAPGHAILAELVWLLSDDAYSYGDIPYQQARAIAIGHA
                     RKAIELAPNSAEGYAALGLVLPPEEGVEPLRRAVRLDPVRAELRAWLALHLATLGRTE
                     EAVREVEAGAEIDPLSTVPVNRLHGIFVASGEFQRAENVVRQYVARGGEPAQAARFRT
                     VQARVSGDLSEAVAQARLAMNLNPGVPYVRQFLEGLAATLGVPALVTWPPERNRRFIW
                     LLAKGDTAKLLLEARSANASVWTKPDSATAFLALAEARDWPQIARLYAARGAATSELC
                     KERTGAIVATAMALERIGRTDESKQLQACLEREIRMQARGPNGPDLTNRNEHEFARAQ
                     LAALRGDGQAALNALEKAVQLGWRGYPYSARLSSYPALESLQSQLGYARIQKRLDQLI
                     AAERSQLALSASPRAAAR"
     gene            complement(4150..5115)
                     /gene="mltG"
                     /locus_tag="VFU20_07625"
     CDS             complement(4150..5115)
                     /gene="mltG"
                     /locus_tag="VFU20_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU5286362.1"
                     /translation="MTKKLAALGVLALFAALVTVWFLWWSPGPRPGPHTVIVEEGSSL
                     ASVARQLEQQGAIPGSARTYRLMARLLGSGDPVQAGEFEIPKGTSGAAVLDLLQHGRP
                     VQRLITVTEGMPSIIVHEKLAANPYLTGPAPLPPEGSVLPDSYGYKRGEARAAVVQRM
                     QAAMTETLDALWAKRKPTCPVNSKEEAVTLASIVEKETGKPSERRMVAGVYCNRLRIG
                     MKLDADPTVIYPITRGKPLGRRIRRSELAADTGYNTYKKPGLPAGPIANPGRESIAAV
                     LDPAPTEAIYFVADGTGGHVFAQSFAEHQANVRKWYAIRRARGEM"
     gene            complement(5112..6371)
                     /gene="fabF"
                     /locus_tag="VFU20_07630"
     CDS             complement(5112..6371)
                     /gene="fabF"
                     /locus_tag="VFU20_07630"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5286363.1"
                     /translation="MRRVVVTGLGLVTPLGGDVETSWKNILASKSGAGPITKFDASDQ
                     KCRIACEVKPAGHEYGFDPARRIDHKIQRQVDDFIIYGLDAAGQAIEDAGLADMSEDM
                     RLRAGCSIGSGIGGLPGIESESLVLHEKGPGRVSPHFVHGRLINLISGQVSIKYGLMG
                     PNHAVVTACSTGAHSIGDAARMIKDDDADIMLAGGAEATICPIGIAGFAQARALSTNF
                     NDQPEKASRPYDRDRDGFVMGEGAGVLVLEEYEHAKKRGAKIYAEVIGYGLSGDAYHV
                     TAPHPEGSGAYRSMEMALKKSGLNPSDIDYINAHGTSTPMGDELELGAVRRLFGNAIG
                     TLSMSSTKSAIGHLLGGAGAVESIFCILAMRDQIVPPTLNLNNPSEGTAGVDLVPHKA
                     KERSVRAVLNNSFGFGGTNASLVMKAV"
     gene            complement(6513..6749)
                     /locus_tag="VFU20_07635"
     CDS             complement(6513..6749)
                     /locus_tag="VFU20_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003039428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5286364.1"
                     /translation="MSETPDRVKKIVVEHLGVDAEKVTEEASFIDDLGADSLDIVELV
                     MAFEEEFGVEIPDDAAEKITTVKDAIDYIDQNKG"
     gene            6866..7315
                     /locus_tag="VFU20_07640"
     CDS             6866..7315
                     /locus_tag="VFU20_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vgr related protein"
                     /protein_id="HEU5286365.1"
                     /translation="MRGSRPLTPGEIALARSIFGDAIDYSKVRMVRRKWWPFQPRGTA
                     MAPTGNIHFHPHGNLWSEDFSAEPLHRQGLFVHEMTHVWQAQTRGRFYLPLMRHPFCR
                     YSYRLEEGRPFDRYGLEQQAEIVRHAFLAERGAPVPCAPPRTLLPFE"
     gene            7456..>7821
                     /locus_tag="VFU20_07645"
     CDS             7456..>7821
                     /locus_tag="VFU20_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5286366.1"
                     /translation="MRISALLLIGTAAIAISVPGLAQTQPEDTAQAADQPASQPAATD
                     VSDEIIVTGTRRLDRTVTNSASPIDVISSAELTNQPTANLLDTIKNIVPSFYVPQNTI
                     SDASTFVRAPSLRGLPADEV"
BASE COUNT         1152 a         2809 c         2629 g         1231 t
ORIGIN      
        1 cgaggcgacc gcgctggtcc gcgacctggt cgacaccccg gccggcgacc tcggcccggc
       61 cgagctcgag gccgcggtcc gcgaccacgt cgccgcggtc ggagccgagg ttcgggtgac
      121 ctccggcgac gcgctcgccg agggctatcc gctggtcgcc gcggtcggcg ccgcggcctc
      181 gcgcgagcgc gcgccgcggc tggtcgaggc cgaatggggc gatcccgggc acccgcgcgt
      241 ggcgatcgtc ggcaagggcg tctgcttcga cagcggcggg ctcgacctca agaccgcgag
      301 cggcatgcgg ctgatgaaaa aggacatggg cggcgcggcg catgcgctgg ccctgttcgt
      361 cctcgccgcc gccgggcgcc tgccggtccg gctgcatttg ctgatcccgg cggtcgagaa
      421 tgcggtatcc gcggccgcgc tccggcccgg cgacatcgtc cggtcgcgca agggcctcca
      481 ggtcgagatc gacaacaccg atgccgaggg ccggctgatc ctcgccgacg cgctcgcccg
      541 ggcggccgag ggcgatggcg aaaagtcccg gcccgagctg atcctcgatt tcgccaccct
      601 gaccggcgcc gcccgggtcg cgctaggccc cgacctgccc gccctgttcg ccaaccgcga
      661 cgagctgctc gacggtttcg aggccgccgc cagggaggtc gaggacccgc tgtggcggat
      721 gccgctgtgg gacggctacg acgacatgct caagagcgac gtcgccgaca tcgccaacgc
      781 caccggatcg cagctggccg gctgcatcac cgccgctctg ttcctgcgcc gcttcgttcc
      841 cggggagacg caatgggcgc atctcgacac gttcgcctgg cgcccgaagg ccaagccggg
      901 gcggcccaag ggcggcgacg cgcttgggct gcgcgccgcc ttcgcgctcc tctgcgagcg
      961 ctatccggca cgctgattcg gccgcggctt cggacgaccc gcgcattttt cccgtttttc
     1021 cggtcgtttg gctgcaacat ttcgcctcct taccggttta attcggcaag cacgcgacgc
     1081 cacggagact tgtcagcaat gagcgaggtt tcgctaaccg gctggatgaa ggcgctgatc
     1141 gcctatgtcc gatccggcga gccggatctc accaaccggc agatggccct gctgatgctg
     1201 gtctatctga cgcctggccc gcacacggtg cgcggcctcg ccaagatcct cggagtctcc
     1261 aaaccggtgg tcactcgcgc cttgaacacg ctcggcgcac tcggctatct gcggcgcgaa
     1321 cgcgaccagg acgaccgccg gaacgtgttc gtcgtccgga ccagcaacgg ggcaaatttc
     1381 cttgaaggct tcaagcgcaa cctccgcgag ggcagcgagg gcgccccggc ggcccaccgg
     1441 ccgatcatcg gcaccgcggc cttcgcccac cagcgctgag ggcttcgcgc tcaccggacc
     1501 gtcgagcccg cccgaccccg cgacccacgc ctaccggcgc gacctcgccg acgtcgcgct
     1561 cgcccaccag gtcatcgcct cccattacgc cgagccgctc gagcgggcgg tgagccgggc
     1621 gggaacgctg cgcgccggcc cgtgcgacga ggccgaggcg ctgctcgagc tcaaccccgg
     1681 cgacccgttc gcgatgctcg acaacagcct cggctgggcg tggggctacg ccggcaagga
     1741 gcgccgcgtc ggctatttgc gaagcgaggc ggtcgaagcg tagcgctgcg ccaacgcgcg
     1801 gcgccacttg aagcgaccgg gccttcggcg cagtctcgcc ccgccggtgc cttggggggg
     1861 cgatgtcccg cgtcttcgtc agctattcac gccaggatgc tccgcgtgcc aaggcgctcg
     1921 ccaaggcgct gcaggcggcc ggccacgagg tctggtggga ctctcacatt cgcggcggct
     1981 cgcgtttcgg agcggagatc gccgaggccc tgaaaaacgc cgaggtggtc gtcgtcctgt
     2041 ggtcgcgctc gtcgatcgat tcggcctggg tcaacgacga ggccgccgaa ggccgggaca
     2101 ccggccggct cgtcccgatc ctgatcgacg attgcaagcc gccgctgggc tttcggcaat
     2161 tccaggcgat cgacctccgg gactggaccg gccggcgaac gcttccgcac catgtcctcg
     2221 acgcgatttc agcgaaaacg gccgatggcc ccttgcctgc tccccgcccg ggcgcggaag
     2281 agcgccgcgg gtggcagctt ggccgacgcc cgctgatcgc cctgctcgcg ctggcggtcc
     2341 tggtcgccgg agcgtgggcc tatttcaacc gcaccagcgc cggcggcgcg gaggtcccga
     2401 ccctcgccgt cctcccgttc gccgacctgt cgcccaaccg ggacaagggc tatttcgccg
     2461 aaggagtcgc ggaatcgatc ttgagcctgc tcggccgcga gcccggcatc cgggtgatcg
     2521 gccgcacctc cgcgtcgctg ttcaaggacc gcggcggcga cctcgccgcg atgcgcaaat
     2581 cgctcggcgt cacccatgtg ctggaaggca gcgcgcgcac cgcgggcgat gacttgcgcc
     2641 tcagcgttcg cctgatcgac gcggccgacg ggacccaatt atgggcccag gactatcagc
     2701 gcaatctccg caatgtgttc gccgtccagg acgaaatcgg caagtcggtg gcgaccgagc
     2761 tgctgggcac gctttcgacg gccggccgga cgcgcgcccc gatcacccgc gcggacgcct
     2821 acgatctata cctcgcgacc cgggcgacca tgcgcaatcg gcgagagccc gtgctccgca
     2881 aagcgctcgg catggcgcag cgggtgatcg ccgccgatcc cgactatgcg ccgggccacg
     2941 cgatcctcgc cgagctggtc tggctgctct cggacgatgc ctattcctac ggcgacatcc
     3001 cctaccagca ggctcgcgcg atcgcgatcg ggcacgcgcg caaggccatc gagctcgcac
     3061 cgaactcggc cgaaggctat gccgccctcg gcctcgtcct gccgcccgag gaaggagtcg
     3121 agccgcttcg gcgagcggtc cgcctcgacc cggtgcgggc cgaattgagg gcctggctgg
     3181 cgctccacct ggcgaccctc ggaaggaccg aagaggcggt tcgggaggtc gaggccggag
     3241 ccgagatcga cccgctgtcg acggtcccgg tcaaccgtct gcacggcata ttcgtggcct
     3301 cgggcgagtt ccagcgcgcc gaaaatgtcg ttcggcaata tgtcgcgcgc ggcggcgagc
     3361 cggcccaggc ggcccggttc cggacggtcc aggcgcgggt gtcgggcgat ctgtccgaag
     3421 cggtcgcgca ggcgaggctg gcgatgaacc tcaaccccgg cgtgccctac gtccggcaat
     3481 tcctcgaggg gctggcggcg acccttggcg tgccggccct ggtgacctgg cctccggagc
     3541 gcaaccgccg cttcatctgg ctactcgcca agggcgacac ggcgaagctg ctgctcgaag
     3601 cccgctcggc caacgcatcg gtgtggacga agcccgattc ggcaaccgca ttcctggctc
     3661 tcgccgaggc gcgcgactgg ccccagatcg cccggctcta cgccgcccgc ggcgccgcca
     3721 cgtcggagct gtgcaaggag cgcaccggag ccattgtcgc caccgccatg gccctcgaac
     3781 ggatcggcag gacggatgaa tcgaagcagc tgcaggcctg cctcgaacgc gagatccgga
     3841 tgcaggcgcg cgggcccaac ggtcccgacc tgaccaaccg caacgaacat gagttcgcca
     3901 gggcgcagct ggcggcgctg cgcggcgacg gccaggcggc cctgaacgcc ctggaaaagg
     3961 ccgtgcagct cggctggcgg ggctatccct actcggccag gctgagcagc tacccggcgc
     4021 tggaaagcct tcagtcccag ctcggctatg cgaggattca gaagcgcctc gaccagctga
     4081 tcgcggcgga acggagccag ctcgccctct ccgcaagccc gcgagcggcg gctcgctgag
     4141 gcccgggttt cacatctcgc cgcgggcgcg gcggatcgca taccatttgc gcacgttcgc
     4201 ctggtgctcg gcgaagcttt gcgcgaacac atggccaccg gtgccgtcgg cgacgaaata
     4261 gatcgcctcg gtcggcgccg ggtcgagcac cgcggcgatg ctctcgcgcc ctggattggc
     4321 gatcggcccg gcgggcagcc ctggcttctt gtaggtgttg tagccggtgt cggcggcgag
     4381 ctccgagcgc cggattcgcc gcccgagcgg cttgccccgg gtgatcgggt agatgaccgt
     4441 cgggtcggca tccagcttca tgccgatccg caagcggttg caatagaccc cggcgaccat
     4501 ccgccgctcc gacggcttgc cggtctcctt ctcgacgatc gaggcgaggg tcaccgcctc
     4561 ctccttcgag ttgaccgggc aggtcggctt gcgcttggcc cacagcgcgt cgagcgtctc
     4621 ggtcatcgcc gcctgcatcc gctgcacgac cgcggcgcgg gcctcgccgc gcttgtagcc
     4681 gtagctgtcg ggcagcaccg agccctcggg cggcagcggc gctgggccgg tcagataggg
     4741 gttcgcggcg agcttctcgt ggacgatgat cgacggcatg ccctcggtga cggtgatcag
     4801 ccgctgcacc ggccggccat gctggagcag gtcgagcacc gcggcgccgc tggtcccctt
     4861 gggaatctcg aactcgccgg cctggaccgg gtcgcccgag cccagcagcc gcgccatcag
     4921 ccggtaggtc cgagcgctgc cggggatcgc gccctgctgc tcgagctggc gggcgacgga
     4981 agcgaggctc gacccctcct cgacgatcac ggtgtgcggc ccgggcctcg ggccggggct
     5041 ccaccacagg aaccagacgg tgaccagcgc ggcgaaaaga gccaggacgc cgagcgcggc
     5101 gagcttcttc gtcacaccgc cttcatgacc aggctggcgt tggtgccgcc gaaaccgaag
     5161 ctgttgttca gcaccgcccg gacggagcgc tccttggcct tgtgcggcac cagatcgact
     5221 ccggccgtgc cctcgctcgg attgttgagg ttgagggtcg gcgggacgat ctggtcgcgc
     5281 atcgcgagga tgcagaagat gctctcgacc gccccggcgc cgccgagcag atggccgatc
     5341 gccgacttgg tcgagctcat cgacagggtg ccgatggcgt ttccgaacag ccgccggacc
     5401 gcgccgagct cgagctcgtc gcccatcggc gtcgaggtgc cgtgggcgtt gatgtagtcg
     5461 atatccgacg ggttgaggcc cgacttcttc agcgccatct ccatcgaccg gtaggcgccc
     5521 gagccctcgg ggtgcggagc ggtgacgtga taggcgtcgc ccgacaggcc gtagccgatc
     5581 acctcggcgt agattttcgc gccgcgcttc ttggcgtgct catattcctc gagcaccagc
     5641 accccggcgc cctcgcccat gacgaagccg tcgcggtcgc ggtcgtacgg ccggctggct
     5701 ttctcgggct ggtcgttgaa gtttgtcgac agcgcccgcg cctgggcgaa gccggcgatg
     5761 ccgatcgggc agatggtcgc ctcggcgccg ccggcgagca tgatgtcggc gtcgtcgtcc
     5821 ttgatcatcc gcgccgcgtc gccgatcgag tgggcgccgg tcgagcaggc ggtgacgacc
     5881 gcgtggttgg ggcccatcag cccgtatttg atcgacacct ggccggagat caaattgatc
     5941 agccggccgt ggacgaaatg cgggctgacc cggcccgggc ccttctcgtg cagcaccagg
     6001 ctctcggact cgatccccgg cagcccgccg atgcccgagc cgatcgagca gccggcgcgc
     6061 agccgcatgt cctcggacat gtcggcgagg cccgcgtcct cgatcgcctg gccggccgcg
     6121 tcgaggccgt agatgatgaa atcgtcgacc tggcgctgga tcttgtggtc gatgcgcctg
     6181 gccgggtcga agccgtactc atggccggcg ggcttgacct cgcaggcgat gcggcacttc
     6241 tggtcgctgg cgtcgaactt ggtgatcgga ccggcgccgc tcttgctcgc caggatgttc
     6301 ttccagctgg tctcgacgtc gccgccgagc ggcgtcacca ggcccagacc agtcaccacg
     6361 acacggcgca tgttcaaact ccaattggaa caggctcgcg cccgttcggc cccggaccgg
     6421 atccggggct gtcgaagcca tctaatgtgt gcctaaacgc ccaactagga aaaagtgcag
     6481 cccttcgaca ggcccggggc gaacggagaa gactatccct tgttctggtc gatatagtcg
     6541 atcgcgtcct tgacggtcgt gatcttctcg gccgcgtcgt ccgggatctc gaccccgaac
     6601 tcctcctcga acgccatcac caattcgacg atgtcgaggc tgtcggcgcc caggtcgtcg
     6661 atgaagctgg cctcctcggt gaccttctcc gcgtcgacgc cgaggtgctc gacgacgatc
     6721 ttcttcaccc ggtcgggagt ctcgctcatg ctcgcattcc tcctgttgct tttgcgctgc
     6781 cctagcgatg ccgcccgcgc ccggcaaggg gcggctgggg ggccgggctt gccgggcgga
     6841 acggcccggc taagggttgc cggcgatgcg cggctcccgc cccctgactc ccggcgagat
     6901 cgccctcgcc cgatcgatat tcggcgacgc gatcgattat tcgaaggtcc ggatggtccg
     6961 gcgcaaatgg tggccgttcc agccccgcgg cacggcgatg gcgccgaccg gcaacatcca
     7021 cttccatccg cacggaaacc tgtggtcgga ggatttctcc gccgagccgc tccaccgcca
     7081 gggcctgttc gtccacgaga tgacccacgt ctggcaggcg cagacccgcg gccgcttcta
     7141 cctgccgctg atgcgccacc cgttctgccg ctactcctac aggctcgagg aaggccgtcc
     7201 gttcgaccgc tacgggcttg agcagcaagc ggaaatcgtc cgccatgcgt tcctcgccga
     7261 gcgcggcgca ccagtgcctt gcgcaccgcc gagaaccctt ctgccattcg aatagtgcgg
     7321 tgcggtgacg gcctctgtaa ccttgctgca acatatgcgg gcggaaagcg cggcctcatg
     7381 cgcgcttccc cttccccccc ggctccgctt aagtcacaga tgcatcgtta cgcgcgctca
     7441 acgggggaat tcatcatgcg tatttcggca ctgctactga tcggcaccgc ggcgatcgcc
     7501 atttcggtac ccggcctggc ccagacccag cccgaggaca ccgcccaggc ggccgaccag
     7561 ccggccagcc agccggcggc gacggatgtc agcgacgaaa tcatcgtcac cggcacgcgc
     7621 cggctggacc gcacggtgac caactcggcc tcgccgatcg acgtgatcag ctcggccgag
     7681 ctgaccaacc agccgacggc gaaccttctc gacacgatca agaatatcgt gccctccttc
     7741 tacgtcccgc agaacacgat ctcggacgca tcgaccttcg ttcgcgcgcc gtctctccgc
     7801 ggcctgccgg ccgacgaggt g
//
LOCUS       DASTZO010000273         3372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_153493,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000273 DASTZO010000000
VERSION     DASTZO010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153493"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..622
                     /locus_tag="VFU20_07650"
     CDS             35..622
                     /locus_tag="VFU20_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU5286367.1"
                     /translation="MQRSSAKLTDEHDAPESLSARADDAAEMAPITGRVKWFDSTRGF
                     GFIVSDEVDGDILLHFSVLREHGRRSVPEGALIECVPMRQERGLQAARVLSIDLSSAL
                     PAQPRSPISASERADRKALAEAAGEFQPVEVKWFNRVKGYGFVNRGGEDEDVFVHMET
                     VRHAGLGELHPGQRLEARVASSPKGLTAVELRAPD"
     gene            640..1092
                     /locus_tag="VFU20_07655"
     CDS             640..1092
                     /locus_tag="VFU20_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HEU5286368.1"
                     /translation="MMLGSSVAACTPGNPAQAPLAERSAAGLEQLPLTIRSATGTHRF
                     TVEVARSPQEQATGLMHRQSLAPDRGMLFPYDPPQPASFWMKNTLIPLDIIFVRADGT
                     IARIAADTVPLSLEPVPSLEPVAAVLEIAGGRAAELGIREGDTVGWPR"
     gene            1174..1557
                     /locus_tag="VFU20_07660"
     CDS             1174..1557
                     /locus_tag="VFU20_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010257 - NADH dehydrogenase complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase subunit NDUFA12"
                     /protein_id="HEU5286369.1"
                     /translation="MGFWSKTFTWWNGATWGTSLFSSRHGKEVGRDEAGNVYFQHKKD
                     PRRRWVIYPGDNDGSNVPPGWNAWLKGTIDELPDKALPARRPFHKPPEKNLTGTMAAF
                     RPDGALGSGKVRPAATGDYQPWTPD"
     gene            1707..1940
                     /locus_tag="VFU20_07665"
                     /pseudo
     CDS             1707..1940
                     /locus_tag="VFU20_07665"
                     /inference="COORDINATES: protein motif:HMM:NF021444.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2155 domain-containing protein"
     gene            complement(1976..2752)
                     /gene="aat"
                     /locus_tag="VFU20_07670"
     CDS             complement(1976..2752)
                     /gene="aat"
                     /locus_tag="VFU20_07670"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HEU5286370.1"
                     /translation="MSRLDPRLLLQCYAAGIFPMADSRDAAELFWVEPRRRAIIPLDG
                     FHMSRSLRRTIRSGRFQITRDRDFAGVIAACADREETWINQEIESAMLALHASGHAHS
                     IEVWNADPRSADRGPQLVGGVYGVRLGRAFFGESMFSTMTDASKVALAWLVARLKVGG
                     FTLLDCQFMTAHLASLGAVSVSRADYVALLSAALGGGSAGGGEAAASFAGSGSPASSG
                     RSAGAPPDFLALDRLLDATGVAGAAGPAGYVIAQLLGQTS"
     gene            complement(2806..3153)
                     /gene="arsC"
                     /locus_tag="VFU20_07675"
     CDS             complement(2806..3153)
                     /gene="arsC"
                     /locus_tag="VFU20_07675"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013832239.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HEU5286371.1"
                     /translation="MKAFIHHNPACGTSRKTLEILRESGADVTVNEYLKSPPDRAELK
                     RLYARAGITPREGLRAKEALAEELGLTRPDVTDDEVLDAMVEHPVLINRPIVETDKGV
                     RLCRPQEKVREIL"
BASE COUNT          537 a         1154 c         1200 g          481 t
ORIGIN      
        1 tggccgacaa tctaacttaa gttaggcagt ccaggtgcag cgtagcagtg ccaagctgac
       61 ggatgagcac gacgcgccgg aaagcctttc ggcgagggcg gacgacgccg ccgaaatggc
      121 gccgatcacc ggccgggtga aatggttcga ctcgacccgc ggcttcggct tcatcgtcag
      181 cgacgaggtc gacggcgaca tcctgctgca tttcagcgtg cttcgcgagc acggccggcg
      241 aagcgtcccc gagggcgcgc ttatcgaatg cgtgccgatg cgccaggagc gcggcctgca
      301 ggcggcccgg gtgctgtcga tcgacctcag ctccgccttg cctgcgcagc ccagatcgcc
      361 catctccgcg agcgaacggg ccgaccgcaa ggcgcttgcc gaagcggccg gggaattcca
      421 gccggtggag gtcaagtggt tcaaccgggt caagggctat ggcttcgtca accgcggcgg
      481 cgaggacgag gacgtgttcg tccacatgga gacggtgcgc cacgcggggc tgggcgagct
      541 tcatcccggc cagaggctgg aggcgcgggt cgcatcgagc ccgaagggcc tgaccgccgt
      601 cgaattgcgg gcgcccgact gatcgccgcc gcggcggcga tgatgctcgg ctcctcagtg
      661 gccgcctgca cccccggcaa tccggcccag gcgccgcttg ccgagcgctc ggcggcgggg
      721 ctcgagcagc tgcctctgac gatccgctcc gcaaccggca cccaccgctt cacggtcgag
      781 gtcgcgcgct ccccgcaaga gcaggcgacc ggcctgatgc accgccagtc gctggcgccc
      841 gaccgcggca tgctgttccc ctacgacccg ccgcagcccg cctcgttctg gatgaagaac
      901 acgctgatcc cgctcgacat aatcttcgtc cgcgccgacg gcacgatcgc ccgaatcgcc
      961 gccgacaccg tgccgctgtc gctcgagccg gtgccctcgc tcgagccggt cgccgcggtg
     1021 ctcgagatcg ccggcgggcg ggccgccgag ctcggcatcc gcgagggcga cacggtgggt
     1081 tggccgcgct agccggcacc ggctcgcctg tcccccggac aggctccgcc ggatgcttgc
     1141 gcgccgcccg cgaagtgggc aaaggccgcg ccgatgggct tctggtcgaa aaccttcacc
     1201 tggtggaacg gcgcgacgtg gggaacctcc ttgttctcga gccgccacgg caaggaggtc
     1261 gggcgcgacg aggccggcaa cgtctatttc cagcacaaga aggatccgcg ccgccgctgg
     1321 gtgatctatc ccggcgacaa tgacggcagc aacgtcccgc ccggctggaa cgcgtggctc
     1381 aagggcacga tcgacgagct tccggacaag gcgctgccgg cgcggcggcc gttccacaag
     1441 ccgccagaga aaaacctgac cgggacgatg gccgcgttcc ggcccgacgg ggcgctcggc
     1501 agcggcaagg tccggcccgc cgcgaccggg gattatcagc cctggacccc tgattgagcg
     1561 tgcgaggggc aatcgccgcc gcgatcgtgc tggcgctcgg cgccggcgct tgcggccgcg
     1621 acgagcaggc ggatcggcag tcgaccgggc gggcggtgac cgcccgggtc gcccagagcg
     1681 ccggcaggcc ggccggggtc accccgatgg agcagcgggt cgccgtgctc ggcctgttga
     1741 acaagcgcaa cgggatcgtc cgcgacctcg ccctgcggcc gggccagtcg gtgcgggtca
     1801 aggacgcgat cgtccggctg cgcgcgtgcg agaccaccgc gccgtgggag agcgaggtcc
     1861 tgaccggcgc cttcgtccag ctcgacgtcg agcgcaccga cggccgctgg cagcgggtgt
     1921 tctcgggctg gctgtacaag gaatcgccgt cgctcaacgt cgtcgagcat ccggtctacg
     1981 acgtctggcc caagagctgc gcgatgacat atccggccgg cccggcggcg ccggcgactc
     2041 cggtcgcgtc gagcagccgg tcgagcgcga ggaagtcggg cggagcgccg gccgagcgcc
     2101 ccgacgatgc gggggagccg gagccggcga acgatgccgc ggcctcgccg ccgccggccg
     2161 agccgccgcc cagcgccgcc gacagcaatg cgacataatc ggcgcgcgag acgctgaccg
     2221 cgcccagcga ggcgaggtgg gcggtcataa actggcagtc gaggagggtg aaaccgccga
     2281 ccttcaggcg cgcgaccagc caggcgagcg cgaccttgga cgcgtcggtc atggtgctga
     2341 acatgctctc gccgaaaaag gcccggccga gcctcacgcc atagaccccg ccgaccagct
     2401 gcgggccgcg atcggcgctg cgcggatcgg cgttccagac ctcgatcgaa tgggcgtggc
     2461 cgctcgcgtg cagcgccagc atcgcgcttt cgatctcctg gttgatccac gtctcctcgc
     2521 ggtcggcgca ggcggcgatg acccccgcga agtcgcggtc gcgggtgatt tggaaccggc
     2581 ccgagcggat cgtccggcgg agcgagcgcg acatgtggaa accatcgagc gggatgatcg
     2641 cccggcggcg cggctcgacc cagaacagct cggcggcgtc gcggctgtcg gccatcggga
     2701 aaatcccggc ggcatagcat tgcagcagca ggcgcgggtc gagccgactc atggccgcac
     2761 tatgcgcgcg cgtgacgcat ctgtcaggcg ccccgccgga cggccctaga ggatctcgcg
     2821 gaccttctcc tgggggcggc acaggcgcac gcccttgtcg gtctcgacga tcggccggtt
     2881 gatcagcacc gggtgctcga ccatcgcgtc gaggacctcg tcgtcggtga cgtcggggcg
     2941 ggtcagtccg agctcctcgg cgagcgcttc cttggcccgc aaaccctcgc gcggcgtgat
     3001 tccggcgcgc gcatagagcc gcttcagctc ggcgcggtcc ggcggcgatt tcaaatattc
     3061 gttgacggtc acgtcggccc cggattcccg caggatttcc aaggtcttgc gcgacgttcc
     3121 gcacgccggg ttgtgatgga tgaaagcctt catcgcggtg ccgtttctag gggctgtcgg
     3181 aggcgcaaag caacggtcgc gcgccggttt ggtggaatca aaggccaagt ttgtcgcgaa
     3241 accggaacga tagtgttcaa tcgtcgttcc tgcgactgtg acacgtgcac cgacgccggg
     3301 gaaatggggt cccggagcat ggtccatgca agctcaataa tcgggagaaa gatgatgaaa
     3361 aaatcaattc tg
//
LOCUS       DASTZO010000274         6704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_127555,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000274 DASTZO010000000
VERSION     DASTZO010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127555"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..260)
                     /locus_tag="VFU20_07680"
     tRNA            complement(184..260)
                     /locus_tag="VFU20_07680"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(224..226),aa:Arg,seq:tct)
     gene            complement(396..1061)
                     /gene="purQ"
                     /locus_tag="VFU20_07685"
     CDS             complement(396..1061)
                     /gene="purQ"
                     /locus_tag="VFU20_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU5286372.1"
                     /translation="MNSAVVVFPGSNCDRDIAVAIEQVTGRAPAMAWHRDSALPDGTD
                     FVALPGGFSYGDYLRSGAMAARAPIMRAVADAAARGVPVIGICNGFQVLTEAGLLPGA
                     LMRNAGLAFVCRPIALTVENSQSLFTAGYRSGETVTFPVAHHDGNYQADEATLDRLEG
                     EGRVAFRYAEAVNGSARSIAGILNDAGNVLGLMPHPERAIEPAHGGTDGRRLFEGLLE
                     AVA"
     gene            complement(1058..1321)
                     /gene="purS"
                     /locus_tag="VFU20_07690"
     CDS             complement(1058..1321)
                     /gene="purS"
                     /locus_tag="VFU20_07690"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU5286373.1"
                     /translation="MKARVFITLKPGVLDPQGKAIHHALEGLGFSGVRDVRAGKLIEL
                     DLADGTGDADIEAMCRKLLANTVIENFRIERLEPAPSQGSAQA"
     gene            1436..1585
                     /locus_tag="VFU20_07695"
     CDS             1436..1585
                     /locus_tag="VFU20_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286374.1"
                     /translation="MVWFAVAMFVVLFGWGAQYFGWSDPSGKVQLALFTSFILGVICG
                     YKNKG"
     gene            1627..2055
                     /locus_tag="VFU20_07700"
     CDS             1627..2055
                     /locus_tag="VFU20_07700"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEU5286375.1"
                     /translation="MTRLPPYAQLLRLRIDDSEGECRFVMPFGEDVVVGRPGYLHGGA
                     IAGLLEFAAFTSLARAIADPAVAMKPITVTVDYMRGGTGRDTFAAAEVQRLGSRIANV
                     EATAWQTERSSPIATARLNFLLERPKRQPAPGLATRPAPA"
     gene            2077..4161
                     /locus_tag="VFU20_07705"
     CDS             2077..4161
                     /locus_tag="VFU20_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010216575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU5286376.1"
                     /translation="MTDVLDRPTTSPISTHRHGDVLVVTSNNPPVNALSAAVRQGLVA
                     AIEEAEADESVKAVVIACEGQTFFAGADITEFGKPPVEPWLPTVVDRIENCSKPVVAA
                     IHGTALGGGLEVALGCHYRVAVPTARLGTPEVKLGLLPGAGGTQRLPRVTGVRKALEM
                     CATGSPISAKEAFDCGLVDRLVEGELVPHAVGYAEEVRSVRPLPKSSEREDRLAEARD
                     HPEIFDEFRQANAKKFRGFDAPEANIRAVQAALDKPYSEGVIEERKLFMELMSGAQAN
                     AQQYFFFAERKAAKIEGLPEDARPRDVHKVGVIGAGTMGGGIAMNFLSAGIPVTIIET
                     SQEALDRGTGVMRKNYEASAARGKLTTEQVSKAMGALTPTLDFAALADCDLVIEAVFE
                     QIEIKKDVFGRLDRIAKPGAILASNTSYLNIDEIAASTSRPQDVVGLHFFSPANIMKL
                     LEVVRGAKTAPDVLLTAMGLAKKIKKVAVVAGVCHGFIGNRMLMPRQVQATQLLLEGA
                     SPEQVDRVHVEFGMPMGPFQMADLAGVDIGWHRDPSRIENVRDALCAIDRWGQKKGAG
                     FYDYDDKRRPTPSPVVQQIIEDFAHRQGVERREISNQEIVERTLYTMVNEGAKILEEG
                     MAQRASDIDVVWVYGYGWPVYRGGPMYWADSEGLATIVEGLKRQEPRLGSDFSFSKLL
                     LDKAAKGEKFTR"
     gene            complement(4151..5347)
                     /locus_tag="VFU20_07710"
     CDS             complement(4151..5347)
                     /locus_tag="VFU20_07710"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5286377.1"
                     /translation="MRPPRPRPKLPRNVWILGFVSLLMDLSSEIYHALLPLFITVTLG
                     LPAAALGAIDGIAEATASFAKLASGRMSDRSRRRKPLILAGYGLAALSKPLFPLASGA
                     AEVMGARFADRLGKGIRGAPRDGMIADETPPEVRGRAFGLRQALDTVGAFLAPLAAIG
                     LMILLAGNIRAVFWIAVIPAMLCFLLAWLALRERAPPHPEQGQRPPFFAGFRELDRAT
                     RRLIAVGFLFTLARFSEGFLILRGFDVGLSATLSPLVLVLFNLAFLALAYPAGSWSDR
                     MSPKAILMIGMAVLVAADLVLAQPLGLPGLAVGVALWGAHMALTQGVFARLIADVAPP
                     HLRATSFGAFYFVGGIGTLLASLGAGLLWDRSGPDATFIAGAAVAAVAMAMLSLLPEQ
                     RRAPSA"
     gene            5419..5829
                     /locus_tag="VFU20_07715"
     CDS             5419..5829
                     /locus_tag="VFU20_07715"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5286378.1"
                     /translation="MFGGSSPAALAARFSAVAQSYIADGPAERAERARRKLLAAIAER
                     EPATLKQVAAAVRRGEPAVSRSVDSLVRAGLVDRSPDPDNRRRLAMRLSDAGRSELER
                     RPSTGGALETRLGRLAHSELRAIERAVEILERTG"
     gene            complement(5834..6130)
                     /locus_tag="VFU20_07720"
     CDS             complement(5834..6130)
                     /locus_tag="VFU20_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HEU5286379.1"
                     /translation="MPRGDKGAYTSKQKRRAEHIEEGYEKRGLSSKEAARRAWATVNK
                     ESGGGKKSGSGRGKTENHSASRKGGRKAASGRTSASRSAAAKKGWETRRRRGNA"
     gene            6259..>6704
                     /locus_tag="VFU20_07725"
     CDS             6259..>6704
                     /locus_tag="VFU20_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5286380.1"
                     /translation="MKLRNVAIIAHVDHGKTTLVDQLFRQSGTFRDNQRVEERAMDST
                     DLEKERGITILAKCTSVDWNGIHINIVDTPGHADFGAEVERILSMVDGVILLVDAAEG
                     PMPQTKFVTGKALALGLKPIVVVNKIDRPDARPAEVLDEIFELFLNL"
BASE COUNT         1096 a         2281 c         2355 g          972 t
ORIGIN      
        1 gccctcctcg tcgggcacgt ccatgtcgtc gcggacatgg aagaagaacc tcgccatgcg
       61 ccgccagcgc tcgctgtgcg ctttcgttcc attgttcaat ttgggttagt cagtctccgg
      121 cgccagcgac cgcgcggcgg cgcgctcgag ccgcgcgtga cgggctccga gctcccgcaa
      181 tgctggcgcg cccggcagga ttcgaacctg cgacctcaag cttagaaggc tcgtgctcta
      241 tccagctgag ctacgggcgc gcgggtcagc aactagcgcg ctttgccggc cagcggaaca
      301 gcgctaccca cggggcaagg catcgcggga aggggcgaat gagcgttctg caaagcggca
      361 agatcatgct catgcttcac ccgggtcctc aagcgctagg cgaccgcttc gagcagcccc
      421 tcgaacagcc gccggccgtc ggtgccgccg tgcgccggct cgatcgcccg ctcggggtgc
      481 ggcatcagcc ccagcacgtt gccggcgtcg ttgaggatcc cggcgatcga gcgcgccgag
      541 ccgttgacgg cttccgcgta gcggaaggcg acccggcctt cgccctcgag ccggtcgagg
      601 gtcgcctcgt cggcctggta attgccgtcg tggtgggcga ccgggaaggt gaccgtctcg
      661 cccgaccggt agccggcggt gaacaggctc tggctgttct cgacggtcag cgcgatcggt
      721 cggcagacga acgcgaggcc ggcgttgcgc atcaaggcgc cgggcagaag cccggcctcg
      781 gtcagcacct ggaagccgtt gcagatgccg atcaccggca ctccgcgcgc cgccgcgtcg
      841 gcgaccgcgc gcatgatcgg cgcccgcgcc gccatggccc ccgagcgcaa gtaatcgccg
      901 taggaaaatc cgccgggcaa ggcgacgaag tcggttccgt ccggaagcgc gctgtcgcgg
      961 tgccaggcca tcgccggggc gcggccggtg acctgctcga tcgccaccgc gatgtcgcgg
     1021 tcgcagttgg agccgggaaa gacgacgacc gcgctgttca cgcctgtgcc gagccttgac
     1081 taggggccgg ctccagccgc tcgatgcgga aattctcgat caccgtgttg gcgagcagct
     1141 tgcggcacat cgcttcgatg tcggcatcgc cggtgccgtc ggcgagatcg agctcgatca
     1201 gcttgccggc gcggacgtcc cggacgcccg agaagccgag cccttcgagc gcatggtgga
     1261 tcgccttgcc ctgggggtcg aggactccgg gcttgagggt gatgaaaacg cgggctttca
     1321 tgatgcgcgc cgctatggcg gcggcgcccc cgccgggcaa gctttacgat ccgtttaccg
     1381 ttcgaggagc atcctgccgc ggccgggggg accggcctgc gatgggagaa agcggatggt
     1441 ctggttcgcg gtcgccatgt tcgtcgtgct gttcggctgg ggagcgcaat atttcggctg
     1501 gagcgatccg tccgggaagg tccagctggc gctgttcacc tcgttcatcc tcggggtcat
     1561 ttgcggctac aagaacaagg gctgaggcgg ctttaagagg tcgccgcggg cgtctacagc
     1621 cgcgcggtga cccggcttcc gccctatgcg cagctgctgc gcctccgcat cgacgacagc
     1681 gaaggcgagt gccgcttcgt gatgccgttc ggggaggacg tcgtcgtcgg gaggccgggc
     1741 tacctccacg gcggcgcgat cgccggcctg ctcgagttcg cagccttcac cagcctcgcc
     1801 cgcgcgatcg ccgatccggc ggtggcgatg aagccgatca ccgtcaccgt cgattacatg
     1861 cgcggcggca ccggccgcga cacgttcgcg gccgccgagg tccagcggct cggaagccga
     1921 atcgccaatg tcgaggcgac cgcctggcag accgagcggt cgagcccgat cgccaccgcg
     1981 cggctcaatt tcctgctcga gcgtcccaag cggcagccgg cgcccggcct tgcaacgcgt
     2041 ccggcccccg cctagcgagc gccggtggag gaagcgatga ccgacgttct cgacagaccg
     2101 accaccagcc ccatctcgac tcaccgccac ggcgacgtgc tcgtggtcac gtccaacaac
     2161 ccgccggtca acgccctgag cgccgcggtc cgccagggcc tggtcgcggc gatcgaggag
     2221 gccgaagccg acgagtcggt caaggcggtg gtgatagcct gcgaaggcca gacctttttc
     2281 gccggcgccg acatcaccga gttcggcaaa ccgccggtgg agccgtggct cccaaccgtc
     2341 gtcgaccgga tagagaattg ttcgaagccg gtggtcgcgg cgatccacgg caccgcgctg
     2401 ggcggcgggc tcgaggtcgc gctcggctgc cactaccggg tcgcggtgcc gaccgccagg
     2461 ctcggcacgc ccgaagtcaa gctcggcctg ctccccggag caggcgggac ccagcggctg
     2521 ccgcgcgtca ccggggtcag gaaagcgctc gagatgtgcg ccaccggcag cccgatcagc
     2581 gccaaggagg cgttcgactg cgggctcgtc gaccggctgg tcgagggcga gctggtgccg
     2641 cacgccgtcg ggtacgccga ggaggtgcgc tctgtccgcc cgctgcccaa atcctccgag
     2701 cgcgaggacc ggctggcgga ggcgcgcgac cacccggaga tcttcgacga gttccgccag
     2761 gccaatgcga agaaattccg cggcttcgac gcgcccgagg cgaacatccg ggcggtccag
     2821 gccgcgctcg acaagcccta ttccgaaggg gtgatcgagg agcgcaagct gttcatggag
     2881 ctgatgagcg gcgcgcaggc caatgcccag caatatttct tcttcgccga gcgcaaggcg
     2941 gccaagatcg aggggctgcc cgaggacgcg cggccgcgcg acgtgcacaa ggtcggggtg
     3001 atcggcgccg gcacgatggg cggcggaatc gcgatgaact tcctgtcggc cgggatcccg
     3061 gtgacgatca tcgagaccag ccaggaggcg ctcgaccgcg gcaccggcgt gatgcgcaag
     3121 aattacgagg cgagcgcggc caggggcaag ctgaccaccg agcaggtgtc gaaggcgatg
     3181 ggcgcgctca ccccgaccct cgacttcgcg gcgctcgccg actgcgacct cgtcatcgag
     3241 gcggtgttcg agcagattga gatcaagaag gacgtgttcg gccggctcga ccggatcgcc
     3301 aagcccggcg cgatcctcgc ctcgaacacg tcctatttga acatcgacga gatcgccgct
     3361 tcgacttcgc ggccgcagga cgtcgtcggg ctgcacttct tctcgccggc caacatcatg
     3421 aagctgctcg aagtggtgcg cggcgcgaag accgccccgg acgtgctcct gaccgcgatg
     3481 ggcctggcca agaagatcaa gaaggtcgcg gtggtcgcgg gcgtctgcca cggcttcatc
     3541 ggcaaccgga tgctgatgcc gcgccaggtg caggcgacgc agctgctgct cgagggcgca
     3601 agcccggagc aggtcgaccg ggtccatgtc gagttcggga tgccgatggg ccccttccag
     3661 atggccgacc tcgcgggggt cgacatcggc tggcaccgcg atcccagccg catcgagaac
     3721 gtccgcgacg cattgtgcgc gatcgaccgc tgggggcaga agaaaggcgc cggattctac
     3781 gattacgacg acaaaaggcg gccgacgccg tcgccggtgg tccagcagat catcgaggat
     3841 ttcgcgcacc ggcagggcgt cgagcgccgc gagatttcca accaggagat cgtcgagcgg
     3901 acgctctaca cgatggtcaa cgagggagcg aagatcctcg aggaaggcat ggcccagcgc
     3961 gcgtcggaca tcgatgtcgt ctgggtctac ggctacggct ggccggtgta ccgcggcggg
     4021 ccgatgtact gggccgacag cgaaggcctc gccacgatcg tcgagggcct gaagcggcag
     4081 gagccgcggc tgggcagcga tttctccttc tccaagctgc tgctcgacaa ggcggcgaag
     4141 ggcgagaagt tcacgcgcta ggagcgcgcc gctgttcggg cagcaggctc agcatcgcca
     4201 tcgccaccgc ggcgaccgcg gcgccggcga tgaaggtcgc gtcggggccg gagcggtccc
     4261 acagcaggcc ggcgccgagg ctggcgagca gggtcccgat cccgccgacg aaatagaaag
     4321 cgccgaagct ggtcgcgcgc aggtgcggcg gggcgacatc ggcgatcaga cgcgcgaaga
     4381 cgccctgggt cagcgccatg tgcgcgcccc acagggcgac cccgacggcg aggcccggca
     4441 gcccgagcgg ctgggcgagc accaggtcgg cggcgaccag caccgccatc ccgatcatca
     4501 ggatcgcctt ggggctcatc cggtcgctcc acgaaccggc cgggtaggcg agggcgagaa
     4561 aggcgaggtt gaacagcacc agcaccagtg gcgagagagt cgccgacagc ccgacgtcga
     4621 acccgcgcag gatcaggaat ccctccgaga agcgggcgag ggtgaacagg aagccgaccg
     4681 cgatcagccg gcgagtcgcc cggtcgagct cgcggaagcc ggcgaagaac ggcggccgct
     4741 ggccttgctc tggatggggc ggcgcccgtt ccctgagcgc cagccaggcg agcaggaagc
     4801 acagcatcgc cgggatcacc gcgatccaga acaccgcccg gatgttcccg gcgagcagga
     4861 tcatcagtcc gatcgcggcg agcggcgcga ggaaggcccc cacggtgtcg agcgcctggc
     4921 gcaggccgaa cgcccggccg cggacttcgg gcggggtctc gtcggcgatc atcccgtcgc
     4981 gcggcgcccc gcggatgccc ttgccgagcc ggtcggcgaa gcgcgcgccc atcacctcgg
     5041 cggcccccga cgcgagcggg aacagcggct tcgacaaagc ggcgaggccg tagccggcga
     5101 ggatcagcgg cttgcggcgg cggcttcggt cggacatccg ccccgacgcg agcttggcga
     5161 acgaggcggt ggcttcggca atgccgtcga tcgcgcccag cgcggcggcc ggaagcccga
     5221 gcgtcaccgt gatgaacaag ggcaggagcg cgtggtagat ttcgctcgac aggtccatca
     5281 gcaggctgac gaagccgagg atccagacgt tgcgggggag cttggggcgg gggcgcggcg
     5341 gtctcaccgg cctgtcatcg cgagccgaat tgtgccggtc aacattgcat cgcccaaaac
     5401 atttgttagg ctgcaattat gttcggggga tcatcaccgg cggcgctcgc cgcgcgcttc
     5461 agcgcggtcg cgcagagcta tatcgccgac ggcccggccg agcgggccga gcgcgcccgg
     5521 cgcaagctgc tcgccgcgat cgccgagcgc gagccggcga ccttgaagca ggtcgctgcc
     5581 gcagtccgcc gcggcgagcc ggcggtcagc cgatcggtcg acagtctggt ccgcgccggg
     5641 ctggtcgacc gttcacccga tcccgacaac cggcggcggc tggcgatgcg gctcagcgat
     5701 gccgggcgca gcgagctcga gcgccggcca agcaccggcg gtgcgctcga gacccggctg
     5761 ggccgactcg cgcacagcga attgagggcg atcgagcggg cggtcgaaat cctcgaacgg
     5821 accggctagc cgctcaggcg ttgccgcgcc ggcggcgggt ttcccatccc ttcttcgccg
     5881 ccgccgaacg gcttgcggag gtgcggccgg acgcggcctt gcggccgccc ttgcgcgacg
     5941 ccgagtggtt ttccgtcttg ccgcggcccg atccggattt cttgccgccg ccggattcct
     6001 tgttgaccgt cgcccaggcc cggcgcgcgg cctccttcga cgacagcccg cgcttctcat
     6061 agccctcttc gatatgctcg gcgcggcgct tctgcttgct cgtgtacgcg cccttgtctc
     6121 cacgcggcat ggctcactcc tccatcgaac aggatgcgcg tttcaacgct tgccgcgttt
     6181 cgaatgtccc atgctgcatt gcaacaaagg cgactttcgg ctagggccgc ggcaccttca
     6241 ctccccgaga ataagcgcat gaaactccgc aacgtggcga tcatcgccca cgtcgatcat
     6301 ggcaagacga cgctggtcga ccagctgttc cgccagtccg gcaccttccg cgacaaccag
     6361 cgcgtcgagg agcgggcgat ggattcgacc gacctcgaga aggagcgcgg gatcaccatc
     6421 ctcgccaagt gcacgtcggt ggactggaac ggtatccaca tcaacatcgt cgacacgccc
     6481 ggccacgccg atttcggcgc cgaggtcgag cgcatcctca gcatggtcga cggggtgatc
     6541 ctgctggtcg acgcggccga ggggccgatg ccgcagacca agttcgtcac cggcaaggcg
     6601 ctggcgctcg gcctcaagcc catcgtcgtc gtcaacaaga tcgaccgacc cgacgcccgg
     6661 ccggccgaag tcctcgacga gattttcgaa ttgttcctca acct
//
LOCUS       DASTZO010000275         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_853894,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000275 DASTZO010000000
VERSION     DASTZO010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_853894"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFU20_07730"
     CDS             <1..295
                     /locus_tag="VFU20_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010413218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation transporter dimerization
                     domain-containing protein"
                     /protein_id="HEU5286381.1"
                     /translation="EWPEEEREAFLAAAGEYPELAGLHDLRTRTSGAHRFVQFHVWVP
                     ADWTVQEAHDRMDRVEEDLQRRFPGTEILIHLDPEGHTDRETMLSSDLTEGAA"
     gene            310..1635
                     /locus_tag="VFU20_07735"
     CDS             310..1635
                     /locus_tag="VFU20_07735"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5286382.1"
                     /translation="MSEPKLERRIGLAGAILLSFNGVLGGGIFAMPAILAVDFGSFSP
                     WLFPLVAFASLLIIVPFARSAGAFPQSGGPATYGAVFGRFVGFELGWIYYIARTAAFA
                     ANANVLTAYVARWWTGADQGVGRAVLMLSVCAILAAVNIAGVKRALALLGGLTLLKAL
                     PLLLAALAVLALTVPWPTPGPMPVLSELEAGVLLVFYAFVGFENVVVPAGETKRPGTV
                     LPRAIFITIALTTLLYFLVQLAYVSAFPGGAAGGSDDKAPLIDLGRWLAGPAGAMVLT
                     LAAIASLSGNLHGIMTSTSRVTSALGERGDLPRWFARVHPLFVTPANSIAFLAAFAAA
                     LALVGTYVWLAVISVLARLIIYAVTIAALPRAPERGTVPRWLYVTGAAGIALCIWAAS
                     QVEWEAWRTLGLLALAGAALYAIARRGASSKAADAVSAIQPPPKSREPS"
     gene            complement(1545..>2391)
                     /gene="mnmA"
                     /locus_tag="VFU20_07740"
     CDS             complement(1545..>2391)
                     /gene="mnmA"
                     /locus_tag="VFU20_07740"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU5286383.1"
                     /translation="IDRFAASYAAGETPIPCVECNQQIKFADLLGTARELGAAVMATG
                     HYVASRARPGGGRALHRAADPDRDQSYFLYGTTRDQLDFLRFPLGDLAKNEVRQLASE
                     AGLAVAAKPDSQDICFVPDGDYAGLVKRLRPETAAPGEIVDLGGRVLGEHRGVVHFTV
                     GQRRGIEVGGQSEPLYVVRIEPERRRLVVGPRRALAVEAMRVEEWNWIGEDQSRVGVK
                     VRSLAPPVAAVIDGGWVRFDRPEYGVAPGQAAVAYDGSRLLGGGWIAETASAALDEAP
                     LLAIA"
BASE COUNT          312 a          858 c          834 g          387 t
ORIGIN      
        1 cgaatggcct gaggaggagc gtgaggcatt cctcgctgct gcaggcgaat atcctgaatt
       61 ggccggcctg cacgacctgc gcacccgcac atcgggagcg catcgctttg tccagttcca
      121 cgtctgggtg ccggccgact ggacagtcca ggaggcgcac gaccggatgg accgggtcga
      181 ggaggacctc cagcggcgct tcccggggac cgagatcctg atccacctcg accccgaggg
      241 ccataccgac cgcgagacca tgctgtcgtc ggacctgacc gagggcgcgg cgtgaccaat
      301 ggcccggccg tgagcgagcc gaaactcgag cggcggatcg gcctcgccgg agcgatcctg
      361 ctgtcgttca acggggtgct cggcggcggc atcttcgcga tgccggcgat cctcgccgtg
      421 gatttcggaa gcttctcgcc gtggctgttt ccgctggtcg ccttcgcctc gctgctgatc
      481 atcgtcccgt tcgctcggag cgccggcgcc ttcccgcaaa gcggcgggcc ggcaacctac
      541 ggagcggtgt tcgggcgctt cgtcggcttc gagctcggct ggatctatta catcgcccgg
      601 accgcggcct tcgccgccaa cgccaacgtc ctcaccgctt atgtcgcgcg ctggtggacc
      661 ggcgccgacc agggcgtcgg tcgggcggtg ctgatgctgt ccgtctgcgc gatcctcgcc
      721 gcggtcaaca tcgccggggt caagcgcgcg ctcgcgctgc tcggcgggct gactctgctc
      781 aaggcgctgc cgctgctgct cgccgcgctc gccgtgctgg ctctgaccgt gccgtggccc
      841 acgcccgggc cgatgccggt gctgagcgag ctcgaggcgg gcgtgctgct ggttttctac
      901 gcgttcgtcg gcttcgagaa cgtcgtcgtc ccggccggcg agaccaagcg gcccggcacc
      961 gtcctgccgc gcgcgatctt catcaccatc gctctgacca ccttgctcta tttcctggtc
     1021 cagctggcct atgtcagcgc gtttcccggc ggtgccgccg gcgggagcga cgacaaagcg
     1081 ccgctgatcg acctcggccg ctggctcgcc gggccggccg gggcgatggt cctgacgctg
     1141 gccgcgatcg cgtcgctgag cggcaatttg cacggcatca tgacctcgac ctcgcgggtg
     1201 accagtgcgc tcggcgagcg cggcgacctg ccgcgctggt tcgcgcgcgt gcatccgctc
     1261 ttcgtcaccc cggccaattc gatcgccttc ctcgccgcgt tcgcggcggc gctggcgctg
     1321 gtcggaacct atgtctggct ggcggtgatc agcgtgctcg cgcggctgat catctacgcg
     1381 gtgaccatcg ccgctttgcc gcgcgcgccc gaacgcggga cggtgccgcg ctggctgtac
     1441 gtcaccggcg cggccgggat cgcgctttgc atctgggcgg cgagccaggt cgaatgggaa
     1501 gcgtggcgga ccctcggcct tctggcgctc gccggggcgg cgctttacgc gatcgcgagg
     1561 aggggcgctt cgtccaaggc cgccgacgcc gtctcggcga tccagccgcc gcccaagagc
     1621 cgcgagccgt cgtaggccac cgccgcctgg ccgggcgcga ctccatattc gggcctgtcg
     1681 aaccgcaccc agccgccatc gatgaccgcc gcgacggggg gcgcgagcga gcgcaccttg
     1741 acgccgaccc ggctctgatc ctcgccgatc cagttccact cctcgacgcg catcgcctcg
     1801 accgccagcg cccggcgcgg gccgaccacc agccggcgcc gctcgggctc gatgcgcacc
     1861 acgtagagcg gctccgactg gccgccgacc tcgatcccgc gcctctggcc gaccgtgaaa
     1921 tggacgaccc cgcggtgctc gccgaggacg cgcccgccga ggtcgacgat ctcgccgggg
     1981 gcggcggttt ccgggcgcag ccgcttgacc agcccggcat aatcgccgtc ggggacgaag
     2041 cagatgtcct ggctgtcggg cttggcggcg accgcaagcc ccgcctcact ggccagttgc
     2101 cgcacctcgt tcttggcgag gtcgccgagc gggaagcgca ggaaatcgag ctggtcgcgg
     2161 gtggtgccgt agaggaaata gctctggtcg cgatcgggat cggcggcgcg gtgcagcgca
     2221 cggccaccgc cgggacgggc acgcgaggcc acataatggc ccgtcgccat gaccgcggcg
     2281 ccaagttcac gcgccgtgcc gagaagatcg gcaaacttga tctgctggtt gcactcgacg
     2341 cagggaatgg gcgtttcgcc ggccgcatag ctggcggcga aacggtcgat c
//
LOCUS       DASTZO010000276         2194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_23255,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000276 DASTZO010000000
VERSION     DASTZO010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2194
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23255"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1303)
                     /locus_tag="VFU20_07745"
     CDS             complement(<1..1303)
                     /locus_tag="VFU20_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HEU5286384.1"
                     /translation="MTKRTGGRILVDQLRIQGCDRIFTVPGESFLAVLDALHDTSEID
                     LVVCRQEGGAAYMADADGKLAGRPGVAFVTRGPGATNASGGVHVAYQDSTPMILFVGD
                     LDRSDKDREGFQEVDFAAFFRPVAKWAARIDDARRIPEYVARAWRVATAGRPGPVVLA
                     IPEDMLRDEVEALDRPPVPPLAEAPDPGAIQALFELLKESCAPVAIVGGADWSPRSAH
                     HFANFAYRYGIPVAAAFRRQDAIDNKSGVYAGQLGYGPNPKLQQRIRDADLILAVGAR
                     LGEATTDGYTLVTPDHPGQTLVHVHPDPNELGRVYHADLPICADMGEFAEMLDEWIDP
                     ELVPFSAGKQAHEEWLEWSEPRPRKGVKLDLGPCVAAMRARLPETTIVCNGAGNFSGW
                     FHRYWRYGKMPTQLAPTSGTMGYGLPAAVAAALRFRDRSVVC"
     gene            1453..>2194
                     /locus_tag="VFU20_07750"
     CDS             1453..>2194
                     /locus_tag="VFU20_07750"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5286385.1"
                     /translation="MGIAARSGWALLATAAAATSAPATPPEDQSYEFRVERAQGALYN
                     PWTGKDDPVELRSFRGSGIAEGAFVAPTIRIAPGRQLTIDIDNKLEPCTAEQAKEHRC
                     FNDTNLHTHGLWVSPSGNSDNVLISIPPGEKFRYQYQIPEDHPAGTFWYHPHRHGSGF
                     VQVGSGMAGALIVTGSRKPTLDRPGDIDILLKDEKGRAFPERVMIFQQIQYGCLDDKG
                     TIDGRLEKVVEDGVEFEEYIAPWLCSAGA"
BASE COUNT          349 a          717 c          799 g          329 t
ORIGIN      
        1 cgcagaccac cgagcgatcc ctgaagcgca acgccgccgc gacggcggcg ggaaggccgt
       61 agcccatggt cccgctggtc ggcgcaagct gcgtcggcat cttgccgtag cgccagtagc
      121 ggtggaacca gccggagaaa ttgccggcgc cgttgcagac gatcgtcgtt tctggaagcc
      181 gggcgcgcat cgccgcgacg cacggaccca ggtcgagctt cacgcccttg cggggcctcg
      241 gttcggacca ttcgagccat tcctcgtggg cctgcttgcc cgcgctgaac ggaaccagct
      301 cggggtcgat ccactcgtcg agcatctcgg cgaactcgcc catgtcggcg cagatcggca
      361 ggtcggcgtg atagacgcgg ccgagctcgt tggggtcggg gtggacgtgg acgagcgtct
      421 gccccgggtg gtcgggcgtg acgagcgtgt agccgtcggt tgtcgcctcg ccgagccgag
      481 cgccgacggc gaggatcagg tcggcgtcgc ggattcgctg ctgcagcttc gggttgggcc
      541 cgtaacccaa ttggccggca taaaccccgc tcttgttgtc tatggcgtct tgacgtcgaa
      601 acgcggccgc gaccgggatt ccgtagcgat aggcgaaatt ggcgaaatgg tgcgcggagc
      661 gcgggctcca gtcggcgccg ccgacgatcg cgaccggcgc gcaggattcc ttcaagagct
      721 cgaacaaagc ctggatcgcg ccggggtcgg gggcttcggc gagcggcggg acgggcgggc
      781 ggtcgagcgc ctcgacctcg tcgcgcagca tgtcctcggg gatcgccaac actacgggcc
      841 ctgggcggcc ggcggtcgcg actcgccagg cgcgggcgac atattcgggg atccgccggg
      901 catcgtcgat ccgcgccgcc catttggcga ccggccggaa gaaagcggcg aagtccactt
      961 cctggaagcc ctcgcggtcc ttgtcggagc ggtcgaggtc gccgacgaac aggatcatcg
     1021 gcgtcgaatc ctgataggcg acgtggactc cgccgctggc gttggtcgcg cccggcccgc
     1081 gggtgacgaa cgcgactccg ggccgcccgg ccagcttgcc gtcggcgtcg gccatgtagg
     1141 cggcgccgcc ctcctgccgg cagacgacga ggtcgatctc gctggtgtcg tggagcgcgt
     1201 cgagcaccgc gaggaagctt tcgcccggca cggtgaagat gcggtcgcag ccctggattc
     1261 gaagctggtc gacgaggatc cggccgccgg tgcgcttggt catggccgcg gctctagcgg
     1321 gtgccgccgc gcttgtggag gggccgcgac cgagcttgcc atggcggccg aaccgagtca
     1381 gacatcggcc cggcgcgcgg gttcgaatgc caggacttgc gcatgcccga gtgagcagca
     1441 ggggagctcg cgatggggat agcggcacga tcgggatggg cgttgctggc aacggcggcg
     1501 gcggcaactt cggcgccggc cacgccgccg gaggaccagt cgtacgaatt ccgggtcgaa
     1561 cgggcgcaag gggcgctcta caacccgtgg acggggaagg acgacccggt ggagctgcgc
     1621 tcgttccgcg gcagcgggat cgccgagggc gccttcgtcg ctccgacgat ccggatcgcg
     1681 cccgggcggc agctgacgat cgacatcgac aacaagctcg agccgtgcac cgccgagcag
     1741 gccaaggagc accgctgctt caacgacacc aaccttcaca cccacggcct gtgggtgtcg
     1801 ccgtcgggga acagcgacaa tgtgctgatc tcgatcccgc cgggcgagaa gttccgctac
     1861 cagtaccaga tcccggagga tcatccggcc ggcaccttct ggtaccaccc gcaccgccac
     1921 ggcagtggct tcgtccaggt cggcagcggc atggccgggg cgctgatcgt caccgggagc
     1981 cgcaagccga cgctcgaccg ccccggcgac atcgacattt tgctcaagga cgagaaaggc
     2041 cgcgcctttc ccgagcgggt gatgatcttc cagcagatcc agtacggctg cctcgacgac
     2101 aaggggacga tcgatgggcg actggagaag gttgtcgaag acggcgtcga gttcgaggaa
     2161 tatatcgcgc cctggctctg ttcggccggc gccg
//
LOCUS       DASTZO010000277         2443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_649590,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000277 DASTZO010000000
VERSION     DASTZO010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2443
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_649590"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="VFU20_07755"
     CDS             <1..478
                     /locus_tag="VFU20_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU5286386.1"
                     /translation="AIIHYLEASHPEPALIPADPKLRGLTIWFEEFADTVLAGCGAKM
                     FYNRVVAPRFLNRPGDQAAADSAERDELPPILDYLETVVPEGDGYLVGDGLTLADIAV
                     ASPFANFGHLGCDLGRERRPRTCAFVERILARPSFAPWVEREAAFLARTAPQPADA"
     gene            complement(534..1070)
                     /locus_tag="VFU20_07760"
     CDS             complement(534..1070)
                     /locus_tag="VFU20_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286387.1"
                     /translation="MANERDDEKFGEKKEFGQDRQQPTGQQGQQPTGQQGQQAEFGQQ
                     GQSREFGQQGQAGSLGSQPGMSGQSPEQTGGQSSGGQSSTGQADLGRSGETETLSSER
                     GSGQGSTGGPSGSEGTGGFVGSQGSGSDDYLREEGSTGGSDFASQGQGATEGQDEDIE
                     TGQRQSRDSDIEGGSGNI"
     gene            complement(1339..2013)
                     /gene="rplI"
                     /locus_tag="VFU20_07765"
     CDS             complement(1339..2013)
                     /gene="rplI"
                     /locus_tag="VFU20_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU5286388.1"
                     /translation="MEVILLERVEKLGAIGDVVTVKNGFARNYLLPNKKALRANEANR
                     KLFEANRKKIEADNADRRAEAEKAAKGVEGKTVQLIRQASNVGHLYGSVSARDIVEAL
                     AAEGAHVTKSQIVLDRPIKAVGMHDVKVALHPEVAVTVRVNVARSPEEADLQLQGIDV
                     MAQMFERDAAPPPEELQPVVETEAAEEAAAEAPEAEAPEAEASGTETPEAGAEAPAAD
                     EGEEQA"
     gene            complement(2025..2249)
                     /gene="rpsR"
                     /locus_tag="VFU20_07770"
     CDS             complement(2025..2249)
                     /gene="rpsR"
                     /locus_tag="VFU20_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU5286389.1"
                     /translation="MARAFFRRRKSCPFSGKDAPKIDYKDVRLLQGFISERGKIVPSR
                     ITAVSTKKQRELAKAIKRARHIGLLPYLVK"
     gene            complement(2253..>2443)
                     /locus_tag="VFU20_07775"
     CDS             complement(2253..>2443)
                     /locus_tag="VFU20_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU5286390.1"
                     /translation="NEDVIRFMTIRVDAHETGPSAMMRRGERDRDGRGERGGRGDRDR
                     GDRGDRVGFRARDEELGV"
BASE COUNT          303 a          874 c          808 g          458 t
ORIGIN      
        1 cgcgatcatc cattacctcg aggccagcca tccggagccg gcgctgatcc cggccgatcc
       61 gaagcttcgc ggcctgacca tctggttcga ggagttcgcc gacacggtcc tcgccgggtg
      121 cggggcgaag atgttctaca accgggtggt tgcgccgcgc ttcctcaacc ggcccgggga
      181 ccaggccgcc gccgactcgg ccgagcgcga cgagctgccg ccgatcctcg attatctcga
      241 gacggtcgtc ccggagggcg acgggtatct ggtcggcgac ggcctgacgc tggccgacat
      301 cgcggtcgcc agcccgttcg cgaacttcgg ccatctcggc tgcgacctcg ggcgcgagcg
      361 ccgtccccgc acctgcgcct ttgtcgagcg gatcctggcg cggccgagct tcgcgccctg
      421 ggtcgagcgc gaggcggcgt tcctcgcccg cacggcaccg caacccgcgg acgcataaag
      481 aaacgccgcg ccccgtaaag gagcgcggcg cccttgtccg aagtcggccg gggctagatg
      541 ttgcccgagc cgccctcgat gtcggagtcg cggctctgcc gctggccggt ctcgatgtcc
      601 tcgtcctggc cttcggtcgc gccctgaccc tggctggcga agtccgaccc gccggtcgag
      661 ccttcctcgc gcagatagtc gtccgagccg ctgccctgcg agccgacgaa tccgccggtt
      721 ccctcgctgc cgctcggtcc gccggtgctg ccctggccgg agccgcgctc gctgctcagg
      781 gtttcggttt caccgctgcg cccgaggtcg gcttgtcccg tcgagctttg gccgcccgag
      841 ctctggccgc cggtttgctc cggggattgg ccgctcatgc ccggctgcga gccaaggctg
      901 ccggcctggc cctgctgccc gaactccctg gactggccct gctggccgaa ttcggcttgc
      961 tgcccctgct ggcccgtcgg ctgctggccc tgctggcccg tcggctgttg ccgatcctgg
     1021 ccgaactctt tcttctcgcc gaatttctcg tcgtctcttt cgttcgccat gctgatcctc
     1081 ctcgcgtcgg cccctcattg ctctgcgggg aacaaaacca agagcatccg ccaaccgttc
     1141 cgcctccgct caagcgaatg cgacgaaccg aaggcattga aacagtgaaa caagttggga
     1201 tcgccggaga atcggctttt ctccgctctc gggcggccag ccggccgtcg ccgggttgcg
     1261 acgaatggat gggaacgcga aaaaagcgcg gccaccgatc gcccggcgcc gcgctcattt
     1321 tcttcggccg aacgcggcct aggcctgctc ttcgccctcg tcggcggccg gagcctcggc
     1381 accggcctcc ggagtttcgg tcccggacgc ttcggcttcg ggagcttcgg cttcgggagc
     1441 ttccgcggcc gcttcttcgg cagcctcggt ctcgaccacc ggctggagct cctcgggcgg
     1501 cggcgcggcg tcgcgctcga acatctgcgc catcacgtcg atgccctgga gctgcaaatc
     1561 ggcttcctcg ggcgagcggg cgacgttgac ccggaccgtc accgcgacct cggggtggag
     1621 cgcgaccttg acgtcgtgca tcccgaccgc cttgatcggc cggtcgagga cgatctggct
     1681 cttggtcaca tgagcccctt cggctgccag cgcctcgacg atgtcgcggg cgctgaccga
     1741 gccgtagagg tggccgacgt tcgacgcctg ccggatcagc tggacggtct tgccctcgac
     1801 gcccttggcg gccttttccg cctccgcgcg ccggtcggcg ttgtcggcct cgatcttctt
     1861 gcggttggcc tcgaacagct tgcggttggc ctcgttggcg cgaagcgcct tcttgttggg
     1921 cagcaggtaa ttgcgagcga agccgttctt gacggtgacg acgtcgccga tcgcgcccag
     1981 tttctcgacc cgctcgagca ggatgacttc catgcttccg ctccttattt cacgaggtag
     2041 ggaagcaggc cgatgtggcg ggcgcgcttg atcgccttgg ccagctcgcg ctgcttcttg
     2101 gtcgacaccg cggtgatccg cgacgggacg atcttgccgc gctcggagat gaacccctgg
     2161 agcaggcgga cgtccttgta gtcgatcttg ggagcgtcct tgccggagaa ggggcagctc
     2221 ttgcggcggc ggaaaaatgc acgggccatg gtctagactc ccagctcttc gtcgcgggcg
     2281 cggaagccga cgcggtcgcc ccggtcgccc cggtcgcggt cgccgcgtcc gccgcgctcc
     2341 ccacgtccgt cacggtcgcg ctcgccgcgg cgcatcatcg ccgaggggcc ggtttcatgc
     2401 gcgtcgacgc ggatggtcat gaagcggatc acgtcttcgt tga
//
LOCUS       DASTZO010000278         8124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_258176,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000278 DASTZO010000000
VERSION     DASTZO010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258176"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="VFU20_07780"
     CDS             complement(<1..132)
                     /locus_tag="VFU20_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004209321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HEU5286391.1"
                     /translation="MDLEKLTERARGFLQAAQTIAVREHHQRIAPAHLLKALLDDEQG
                     "
     gene            complement(201..1007)
                     /locus_tag="VFU20_07785"
     CDS             complement(201..1007)
                     /locus_tag="VFU20_07785"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEU5286392.1"
                     /translation="MSLLIALSIAASAQPVAAPPPPLGWQPAAAYVTAGQDEPGYRAW
                     YMASPRHGYAVKSFNHYLVTYGVGGIVPTWQLLRTASSWQRCGAQPFEVPPTSEWPNV
                     VQTLRYVRDFVIPAVGPVEPVSAYRNPVLNVCAGGAPESAHKHYSAIDLVPLRPTNRE
                     ELMRTLCAVHPRRGPDYGVGLGFYAFLRFHVDTTKYRRWGADPGSTECPPIIRPSDVA
                     SVYQPPQAPQPPVVAPAPALADPLAPVPSSALPPAETVPAASEPALPPQR"
     gene            1070..1387
                     /locus_tag="VFU20_07790"
     CDS             1070..1387
                     /locus_tag="VFU20_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoic acid system family protein"
                     /protein_id="HEU5286393.1"
                     /translation="MQQPIQVDLPHKLGRDEARRRIANNIHKLKEHIPGGASHVDSHW
                     AGDELDLTVHALGQAVATKIGVEETKVRVRVMLPGMLAIFAKPIEAMLQNKGDLLLED
                     KSD"
     gene            1595..2899
                     /locus_tag="VFU20_07795"
     CDS             1595..2899
                     /locus_tag="VFU20_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5286394.1"
                     /translation="MRHFAVVGSGPAGFYTAEALAKAYGEQARIDIIDRYPVPYGLIR
                     FGVAPDHQSLKSVSKRYEKVAETDGVGFIGNVCVGSDVSVAELLEQYDSVILAIGAPH
                     DRKLGIPGEELPGVVGSAEFVGWYNGHPDFADLDPPLGGGHAAVIGNGNVALDVARIL
                     AKTRAEFEGSDIVGHALEALDTSAIRTITILGRRGPHQIAMTPKELGELAHLEAALPV
                     VDLADFPPVEADDALDPGQRKSVTILREFANIRANKPKTMLFDFFARPVSIEGEGRAE
                     RVIVERTELDDKGSARGTGETYEVPASLVVSCIGYSTPPLGGVAYDERGGKFLNEAGR
                     IADRLYCVGWARRGPTGTIGTNRPDGYEVAEAIAAALPPGSDGGRSGAEGLKRLLSGR
                     GVQATDFADWRRIEAAEEAAARPGSPREKMVRAEQWLQTLGL"
     gene            2991..3067
                     /locus_tag="VFU20_07800"
     tRNA            2991..3067
                     /locus_tag="VFU20_07800"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3025..3027,aa:Pro,seq:cgg)
     gene            complement(3559..4374)
                     /locus_tag="VFU20_07805"
     CDS             complement(3559..4374)
                     /locus_tag="VFU20_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEU5286395.1"
                     /translation="MNRVDDINRGISGIKALESVEIPSLEGKVSAEEWAIRIDLAAAY
                     RLVAHYGWDDLIFTHLSARIPGPEHHFLLNPYNLMFEEVTASSLVKVGMDGNPVEPTP
                     FITNPAGFTIHSAIHMAREDAQAVMHLHTPAGQAVAAHADGLLPLTQTAMLVRGDLAF
                     HDYEGVAVDLDERERIVADLDDKNAMILRNHGTLAVGPSVGECFVRLYFLERACQAQI
                     MALSAGEAINNPPQGSPEITAQQGQAGVALAANFLAWPALKRKAFRLDPGFAS"
     gene            complement(4371..5012)
                     /gene="maiA"
                     /locus_tag="VFU20_07810"
     CDS             complement(4371..5012)
                     /gene="maiA"
                     /locus_tag="VFU20_07810"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HEU5286396.1"
                     /translation="MSRPVLHDYYRSSAAYRVRIALNLKGVDYESRPVNLAAGEQKSA
                     AYRALNPQGFVPMLEIDGLALTQSLAIIVYLDQHFPEPPLMPAGVAEQARVRALALSI
                     ACDIHPLNNLRVLKYLEEPLGADKEARDAWYRHWVSEGFAALEAMAASDAGDFLFGDA
                     PSLADVCLVPQMYNARRFDVPLDAYPTLLRADASANRLDAFEHAHPDRQEAPK"
     gene            complement(5023..5427)
                     /locus_tag="VFU20_07815"
     CDS             complement(5023..5427)
                     /locus_tag="VFU20_07815"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU5286397.1"
                     /translation="MTRPAFELAFTVGREHIDELGHVNNAVWVQWIQEVAVAHWESVA
                     DPAHKDAYYWVIVRHEIDYLRPAFEGDRVVGRTWVGDAPKGARFDRHMEFTGNDGRLC
                     VRARTFWAIVDKASGRPIRVPSEVVAPFLGQA"
     gene            complement(5424..5828)
                     /locus_tag="VFU20_07820"
     CDS             complement(5424..5828)
                     /locus_tag="VFU20_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease domain-containing protein"
                     /protein_id="HEU5286398.1"
                     /translation="MLRTTKRTVANARSLRRALTLPEGLLWRALRRRPSNLKFRRQHP
                     CGPFVLDFYCESAKLGIEVDGMAHDMGARPSRDAGRDLWLSEQGIRVLRIPARHVLQD
                     LEAVIRQIIAACHPSTTPLRVAVPLPFREEFE"
     gene            complement(5887..7944)
                     /locus_tag="VFU20_07825"
     CDS             complement(5887..7944)
                     /locus_tag="VFU20_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017671476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5286399.1"
                     /translation="MTQLPDPPKAEQRPYSYQAHGETIEDPWHWLRDPGYPQVKDEAV
                     LAYLQAENAYFQAAMQPHRALVDTLFEEMKGRIKEDESSVPIRDGEWLYWWAFAPGAQ
                     YRSWYRRKFAGGPDEMLFDEPAEAEGKEYFRLGALEVSPDGRLLAVLVDDNGSERFKL
                     RIRDIATGTDLETVTDVAIGQPVWSSDSAGIVFNEVNENWRSYRARYHRLGRPTHEDV
                     TLYEESEELGFTVHVSRSQDRELIFISTGDNATTEVRFVPASDPTAPPVMISPRQEKR
                     EYSVDSAHGKLWIVTNDDHVNFRLAEADPAAPGEWRTVIPGSERVYLRGATAYRDHLA
                     ISQRVDGLDQLVLRTYSGEETRIPFGEASYSAGFGGNPEFAPAAYRLVYSSMVTPQTV
                     YDYHPASGELETLKVQEIPSGYDASKYSTERLMVPARDGQRIPVSVVYPKGFKKDGTG
                     RLLLYAYGAYGYAVPPSFSTSRISLLDRGWAFAIAHIRGGDDLGYDWFLKGKLEHRTN
                     TFNDFVDAAKGLIAQGFTKAGNIAIQGGSAGGELMGAVANSDPELWGAVVADVPFVDV
                     LNTMLDDTLPLTPGEWPEWGNPITDQKAFALIKSYSPYDNVTPQAYPPMLITGGLNDP
                     RVTYWEPAKWAARLRATKTDDNLLLLKINMGAGHGGKSGRWDHLKEIAEAYAFMLTQV
                     GGA"
BASE COUNT         1284 a         2725 c         2796 g         1319 t
ORIGIN      
        1 cccctgctcg tcgtcgagca gcgccttgag caaatgcgcg ggcgcgatcc gctggtgatg
       61 ctcgcgcacc gcgatcgtct gggcggcctg caggaagccg cgcgcgcgtt cggtcagttt
      121 ctccaggtcc atcggtcacc cctgtgcatc gccgccgata tggcgttgca catcggcaac
      181 acaaggggaa agcgcagccg ctaccgttgc ggcgggaggg cgggctcgga ggccgcgggg
      241 accgtctccg cgggcgggag cgcggacgaa ggcaccggcg cgagcggatc ggccagcgcc
      301 ggcgccggcg cgacgaccgg cggctgcggt gcctgcggcg gctggtagac cgaggcgacg
      361 tcggacgggc ggatgatcgg cgggcattcg gtcgagcccg ggtcggcccc ccagcgccgg
      421 tacttggtcg tgtcgacgtg gaacctcagg aacgcgtaga agccgaggcc aaccccatag
      481 tcggggccgc gccgcgggtg caccgcgcac agcgtccgca tcagctcctc gcgattggtc
      541 ggccgaagcg gcaccaggtc gatcgccgaa taatgcttgt gggcgctttc cggcgcgccg
      601 ccggcgcaga cgttgagcac cgggttgcga taggcggaca ccggctcgac cgggccgacc
      661 gccgggatca cgaagtcgcg cacgtagcgc aaggtctgga cgacgttcgg ccattcggag
      721 gtcggcggca cctcgaacgg ctgggcgccg cagcgctgcc acgacgaggc ggtgcgcagc
      781 agctgccagg tcgggacgat cccgccgacc ccgtaggtga cgaggtagtg gttgaagctt
      841 ttgaccgcgt agccgtgccg cggcgaggcc atgtaccagg cgcggtagcc gggctcgtcc
      901 tgccccgcgg tcacataggc cgcggccggc tgccagccga gcgggggcgg cggggccgcg
      961 accggctgcg ccgaggcggc gatcgacagg gcaatcagca ggctcatccg cgcagcataa
     1021 tggacggtcc cagcttgaaa agcgatgaac ggccgcccac tcttcgctca tgcaacagcc
     1081 gatccaggtc gacctgccgc acaagctggg ccgcgacgag gcccggcggc gcatcgccaa
     1141 taacatccac aaattgaagg agcatatccc gggcggcgcg tcccacgtcg attcgcactg
     1201 ggccggcgac gagctcgacc tcaccgtcca cgcgctcggc caggcggtcg cgacgaagat
     1261 cggcgtcgag gagaccaagg tccgggtccg ggtgatgctg cccggcatgc tggcgatctt
     1321 cgcgaagccc atcgaggcga tgctccagaa caagggcgac ctgctgctcg aggacaaaag
     1381 cgactgaatt cctccccgga acggggaggg ggaccagcgc agctggtgga ggggcccctc
     1441 accctccgtc gcatcggccc ccacgaaagt actactttcg tgggaaccct gcgctcctcc
     1501 tccctctccc gcgcgcggga gagagtcggg gtgagggctc ttgccccctt cgttgcagcc
     1561 tctagcatcg gcgcacaatg atcgggggca gctgatgcgg catttcgcgg tggtcgggtc
     1621 gggaccggcg ggcttctaca ccgccgaggc gctggccaag gcgtacggcg agcaggcccg
     1681 catcgacatc atcgaccgct acccggtgcc ctacgggctg atccgcttcg gggtcgcccc
     1741 cgaccaccag tccttgaaat cggtttccaa gcgctacgag aaggtcgccg agaccgacgg
     1801 cgtcggcttc atcggcaatg tttgcgtcgg aagcgatgtc tcggtcgccg agctgctcga
     1861 gcaatatgat tcggtgatcc tcgccatcgg cgcgccgcac gaccgcaagc tcggaatccc
     1921 cggcgaggag ctgcccggag tggtcggctc ggccgagttc gtgggctggt acaacggcca
     1981 ccccgatttc gccgacctcg atccgccgct cggcggcggc cacgcggcgg tgatcggcaa
     2041 cggcaacgtc gccttggacg tcgcccggat cctcgccaag actcgggccg agttcgaggg
     2101 ctcggacatc gtcggccacg cgctcgaggc gctcgacacg tcggcgatcc ggaccatcac
     2161 catcctcggc cgccgcggcc cgcaccagat cgccatgacc cccaaggaat tgggcgagct
     2221 cgcgcacctc gaggcggcgc tgccggtcgt cgacctcgcc gacttcccgc cggtcgaggc
     2281 cgacgatgcg ctcgatcccg gccagcgcaa gtcggtcacc atcctgcgcg agttcgcgaa
     2341 catccgcgcc aacaagccca agacgatgct gttcgacttc ttcgccaggc ccgtttcgat
     2401 cgaaggggag ggccgggccg agcgcgtgat cgtcgagcgc accgagctcg acgacaaggg
     2461 ctcggcgcgc ggcaccggcg agacctacga ggtccccgcc tcgctggtcg tcagctgcat
     2521 cggctactcg accccgccgc tgggcggcgt cgcctacgac gagcgcggcg gcaagttcct
     2581 caacgaggct ggccggatcg ccgaccgcct ctattgcgtc ggctgggcgc ggcgcgggcc
     2641 gaccgggacc atcggcacca accgccccga cggctatgag gtcgccgagg cgatcgccgc
     2701 cgcgcttccg cccggcagcg atggcggccg gtccggcgcc gaaggcctca agcgcctgct
     2761 gtcggggcgc ggagtccagg ccaccgactt cgccgactgg cgcaggatcg aggccgccga
     2821 ggaagccgcc gcccgccccg gcagcccgcg cgagaaaatg gtccgcgccg agcagtggct
     2881 gcagacgctc ggattgtaga ttgagcgggg cagggcgcgg gttgctcttt gccggccccc
     2941 catctatcta gccgcggcct tggctcccgc gacaaacggc gggttccggt cggggagtag
     3001 ctcagcctgg tagagcactg tcttcgggag gcaggggccg gaggttcgaa tcctctctcc
     3061 ccgaccactt ttcgttcgac gctaactttt gcctaatgcg aaagagctaa gcgggcgcga
     3121 tgaacgcgcc agcattcatt gaaccgagct tgagttcagc ccaccgcgcc gcggtcgagg
     3181 aggcgaactg ctggcgcgga cgttgcgtca acttgtttgc gcgcggagaa tttgcggtcg
     3241 gatctgcact ccgcgcgcga cagcccgccg ccaggatgcc gatgctgatt agtcaaaaag
     3301 ttcaacgctt gttcaacgag ctgacgggac ccgaagttcg caagcgtttg gatgcgttcg
     3361 gggcgctgct cgcggagcgc acggctctag cccatggggt cggcaagcct tgggtagaca
     3421 agtctgggac ctggctcctg acgctggaat gggtttccaa caaaggattg gagcgccgtg
     3481 tatttacggc gacggaggca atgcaatttc acgataagtt gcggaccgcc gttcagcgcg
     3541 tcgaggcggc gctcaaacct agctcgcgaa ccccgggtcg agccggaacg ccttgcgctt
     3601 gagcgccggc caggcgagga aattggcggc cagcgcgact ccggcctggc cctgctgcgc
     3661 ggtgatctcg ggcgagccct ggggcgggtt gttgatcgcc tcgccggccg acagcgccat
     3721 gatctgcgcc tggcacgcgc gctcgaggaa atagagccga acgaagcatt cgccgacgct
     3781 cgggccgacc gccagcgtgc cgtggttgcg caggatcatc gcattcttgt cgtccaggtc
     3841 ggcgacgatg cgctcgcgct cgtcgaggtc gaccgcgacc ccctcgtaat cgtggaacgc
     3901 gaggtcgccg cggaccagca tcgccgtctg ggtcagcggc agcagcccgt cggcgtgcgc
     3961 ggcgaccgcc tggccggccg gcgtgtgcag gtgcatcacc gcttgcgcgt cctcgcgcgc
     4021 catgtggatc gccgaatgga tagtgaagcc ggccgggttg gtgatgaacg gcgtcggctc
     4081 gaccgggttg ccgtccatgc cgaccttgac cagcgacgag gcggtcacct cctcgaacat
     4141 gaggttgtac ggattgagca ggaaatggtg ctcgggcccc gggatccggg ccgacagatg
     4201 ggtgaagatg aggtcgtccc agccgtaatg cgcgaccagc cggtaggcgg ccgcgaggtc
     4261 gatgcggatc gcccattcct cggcgctcac cttgccctcg agcgacggaa tttcgaccga
     4321 ttccagggcc ttgatgccgc ttattccgcg gttgatgtcg tcgacccggt tcatttcggc
     4381 gcctcctgcc ggtcggggtg cgcgtgctcg aacgcgtcca gcctgttcgc gcttgcatcc
     4441 gcccgaagca aggtcggata ggcgtccagg ggcacgtcga agcgccgcgc attgtacatc
     4501 tgcggcacca ggcagacgtc ggcgaggctc ggcgcgtcgc cgaacaggaa gtcgcctgcg
     4561 tccgaggccg ccatcgcctc cagcgccgcg aagccctcgc tcacccagtg gcggtaccag
     4621 gcgtcgcgcg cctccttgtc cgcgcccagc ggctcttcca gatatttgag cacgcgcaga
     4681 ttgttgagcg ggtggatgtc gcaggcgatg gacagcgcca gagcccgcac ccgcgcctgc
     4741 tcggcgacgc ccgcgggcat cagcggcggc tcggggaaat gctggtcgag atagacgatg
     4801 atcgccaggc tctgggtcag cgcgagcccg tcgatctcga gcatcggcac gaagccttgc
     4861 gggttgagcg cgcggtatgc ggcggatttc tgctcgccgg cggccaggtt cacgggccgg
     4921 ctctcgtaat cgacgccctt caaattgagc gcgatccgca cccggtaggc ggccgacgag
     4981 cggtagtaat cgtggaggac cgggcggctc acgggcccga gcttacgctt gcccgaggaa
     5041 cggcgccacc acctcgctcg gcacccggat cggccggccc gacgccttgt cgacgatcgc
     5101 ccagaaggtc cgcgcccgga cgcagagccg gccgtcgttg ccggtgaatt ccatgtggcg
     5161 gtcgaacctt gcccctttgg gggcgtcccc gacccaggtg cgcccgacca cccggtcgcc
     5221 ctcgaaggcg gggcggaggt aatcgatctc gtgccggacg atgacccaat aataagcgtc
     5281 cttgtgcgcc gggtcggcga ccgactccca atgcgcgacc gcgacctcct ggatccactg
     5341 cacccagacc gcattgttga cgtgcccgag ctcgtcgatg tgctcgcgcc cgacggtgaa
     5401 cgccagctcg aacgccgggc gggtcactca aattcctccc tgaaggggag ggggaccgcg
     5461 acgcgcagcg gcgtggtgga ggggtggcac gcagcgataa tctgacgaat cacggcctcg
     5521 aggtcctgaa gcacatggcg cgccgggatg cgcagcacgc gaatgccttg ctccgacaac
     5581 caaaggtcac ggccggcatc gcgactcggc cgagccccca tgtcgtgcgc cattccgtcg
     5641 acttcgatgc cgagcttggc gctttcgcag tagaaatcga gaacgaaggg gccgcaggga
     5701 tgctggcgac gaaatttgag attgctcggt cgccggcgca acgcgcgcca gagcagtccc
     5761 tccggcaaag tcaacgcgcg tcggagagac cgcgcattcg cgaccgtcct cttcgtcgtt
     5821 ctcagcattg cccctccacc atgctgcgca tggtccccct cccctgatcg atcaggggag
     5881 gaattgtcac gcgccgccca cctgcgtcag catgaaggcg taggcttcgg cgatctcctt
     5941 caaatggtcc cagcggcccg acttgccgcc gtggccggcg cccatgttga tcttcagcag
     6001 cagcagattg tcgtcggtct tggtcgcgcg cagccgggcc gcccatttcg ccggctccca
     6061 gtaggtgact cgcgggtcgt tgaggccgcc ggtgatcagc atcggcggat aggcctgcgg
     6121 agtcacattg tcgtacggcg aatagctctt gatcagcgcg aacgctttct ggtcggtgat
     6181 cgggttgccc cactcgggcc actcgcccgg ggtcagcggc agagtgtcgt cgagcatggt
     6241 gttcaagacg tcgacgaacg gcacgtcggc gaccaccgcg ccccacaatt ccgggtcgct
     6301 gttggcgacc gcgcccatca gctcgccgcc cgccgagccg ccctggatgg cgatgttgcc
     6361 ggccttggtg aagccttgcg cgatcagtcc cttcgcggcg tcgacgaagt cgttgaaggt
     6421 gttggtgcga tgctcgagct tgcccttcag gaaccagtcg tagccgaggt cgtcgccgcc
     6481 gcggatgtgg gcgatggcga acgcccagcc gcggtcgagc aagctgatcc ggctggtcga
     6541 gaagctcggc ggaaccgcat agccgtaggc gccgtaggcg tagagcagca gccggcccgt
     6601 gccgtctttc ttgaagccct tcgggtagac gaccgacacc gggatccgct ggccgtcgcg
     6661 cgccggcacc atcagccgct cagtgctgta ttttgacgcg tcgtagcccg acgggatctc
     6721 ctggaccttg agggtctcca gctcgccgct ggccggatgg tagtcgtaga cggtctgcgg
     6781 ggtgaccatc gacgaataga ccaggcggta ggcggccgga gcgaactcgg ggttgccgcc
     6841 gaagccggcg ctgtagctcg cttcgccgaa cgggatccga gtctcctcgc cggaataggt
     6901 gcgcagcacc agctggtcga gcccgtcgac ccgctgcgag atggcgagat ggtcgcggta
     6961 cgcggtcgcc ccgcgcaggt agacgcgttc cgagccgggg atcaccgtcc tccactcgcc
     7021 gggcgccgcc gggtcggcct cggcgaggcg gaagttcaca tggtcgtcgt tggtgacgat
     7081 ccacaatttc ccgtgggccg aatcgaccga atattcgcgc ttttcctggc gcggcgagat
     7141 catcaccggc ggcgcggtcg ggtcggacgc ggggacgaag cgcacctcgg tggtcgcatt
     7201 gtcgccggtc gaaatgaaga tcagctcgcg gtcctgcgag cgcgacacgt ggacggtgaa
     7261 gccgagctcc tcactctcct cgtagagggt cacgtcctcg tgcgtggggc ggccgagccg
     7321 gtggtagcgc gcccggtagc tgcgccaatt ctcgttgacc tcgttgaaca cgatgccggc
     7381 gctgtcgctc gaccagaccg gctggccgat ggcgacgtcg gtcaccgtct cgaggtccgt
     7441 gcccgtcgcg atgtcgcgga tcctgagctt gaagcgctcc gagccgttgt cgtcgaccag
     7501 caccgcgagc agccggccgt cggggctgac ctcgagcgcg cccaggcgga aatattcctt
     7561 gccctcggcc tcggccggct cgtcgaacag catctcgtcc ggcccgccgg cgaatttgcg
     7621 ccgataccag ctgcggtatt gcgcgccggg cgcgaacgcc caccagtaga gccactcgcc
     7681 gtcgcggatc gggaccgagc tctcgtcctc cttgatccgc cccttcatct cctcgaacag
     7741 agtgtcgacc agcgcccggt gcggctgcat cgccgcctgg aaataggcgt tctcggcctg
     7801 gaggtaggcg agcaccgcct cgtccttgac ctgcgggtag ccggggtcgc gcagccagtg
     7861 ccacgggtcc tcgatcgtct cgccgtgcgc ctggtagctg taggggcgct gctcggcctt
     7921 gggcgggtct ggaagctggg tcacacatta ctccgttcgg gctgggcctg tcgaagccct
     7981 gcactttctt ctaggtgcct agtaagaagg accgtccttc gacaagctcg ggacgagcgg
     8041 agaatgtgaa atgtcgagtt atgcggaccg tttgaaggcc cttcgcgaac aattgaaggc
     8101 cgaccgcctc gacggcttcg tcgt
//
LOCUS       DASTZO010000279         2778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1120221,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000279 DASTZO010000000
VERSION     DASTZO010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1120221"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..291
                     /locus_tag="VFU20_07830"
     CDS             115..291
                     /locus_tag="VFU20_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286400.1"
                     /translation="MKKTDYRGTADAGSDKRTQPRGGDDPVRRARQWGGGSKGSKGAV
                     TAADRANDNSSARS"
     gene            304..465
                     /locus_tag="VFU20_07835"
     CDS             304..465
                     /locus_tag="VFU20_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286401.1"
                     /translation="MKSSQGRHGGGGGRTDKGEARNERAQAARNTDVDSLARDRNEHR
                     AGGKAAGKA"
     gene            544..1542
                     /locus_tag="VFU20_07840"
     CDS             544..1542
                     /locus_tag="VFU20_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017669295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HEU5286402.1"
                     /translation="MSFVRTLAAAAALVLTAAPACAQAPKTDTPATATTRVFQLPEGA
                     RVHDVAPAPDGTIWYTAQRQGALGILDPKTGKVRHIPLGEGSAPHGVIQGPDGAAWIT
                     DSGLNAIVRFDPKSEKLTVWKLPEDAGYANLNTGAFDGDGIHWFTGQNGIYGRLDPKT
                     GAMRIFSDPDGRGPYGIAATPSGDVYYVSLAGSHLAKIDRKTGAAAIVEPPTPAQGAR
                     RVWADSKGNLWVSEWNSGNLSRYTPATNTWTMWKLPGDKPKAYAVYVDERDIVWVSDF
                     AANAVLAFDPRTQTWTKYPGSGEGANVRQILGAPGEVYLPESGTDRIMVVRTARPG"
     gene            1557..1934
                     /locus_tag="VFU20_07845"
     CDS             1557..1934
                     /locus_tag="VFU20_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5286403.1"
                     /translation="MAPAVLLATAAVAGPPSKPPSKGERAYQKCYSCHELGRGAGQLE
                     GPTLRRIVGRRVAAERGFAYSPALRRFARRHPRWTLKLLDRYVANPEALVPGTSMNFH
                     GISDAAERRALVEYLNGAGRSSR"
     gene            2101..>2778
                     /locus_tag="VFU20_07850"
     CDS             2101..>2778
                     /locus_tag="VFU20_07850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286404.1"
                     /translation="MKRTIVTALAAASLLTLSACSQPAQDTPAEEQAAAAEGIEGTWK
                     SDLASVQLEDEPNVYLIKDGQYVCSTCNPPLTTPADGKFHAVADRPYFDSMAVTVVDD
                     RTVKFERKMGDRDVGSSTWQLSADGNTLNVEFVDKTAANPVTGKGSSTRVAAAPAGAH
                     ATSGSWKANPVSNLSDEALTFSYDLEGDTIRNRSGDGTSYEAKIGGPDAPVQGDPSGL
                     MVAVTRPS"
BASE COUNT          508 a          962 c          956 g          352 t
ORIGIN      
        1 gaaatcgccg gcgagaacac cgtcgaaggg gacgtcgaca atgattcgac cccgaccgac
       61 ggggtccccg agcgcgagcg cggccggacc aacgcctgac cgggagatcc aaagatgaaa
      121 aagaccgatt atcgaggaac ggccgatgcc ggcagcgaca agcgcaccca gccccgcggc
      181 ggcgatgacc cagtccggcg cgcgcgccaa tggggcggcg gctcgaaggg ctcgaaaggc
      241 gcggtgaccg cggccgaccg agccaacgac aacagcagcg ccaggagctg agagggaagc
      301 gtcatgaaaa gcagtcaagg ccgccatggc ggcggcggcg gcaggaccga caagggcgag
      361 gcgaggaacg agcgggccca ggcggcccgc aacaccgacg tcgacagcct tgcccgcgac
      421 cgcaacgagc atcgcgccgg aggcaaggcg gccggcaagg cgtagtcgac gccgggcgaa
      481 cgtcttgcgc gtatcggcgc ggctccgcat agtcgcgccc gacctttgcg ggagaaagcg
      541 ccgatgagct ttgtccggac gctggccgcg gcggcggcgc tggtgctgac ggcggccccg
      601 gcttgcgccc aggctcccaa gaccgacacg cccgcgacgg cgacgacgcg ggtcttccag
      661 ctgcccgagg gcgcccgggt ccacgacgtc gccccggcgc ccgacggcac gatctggtac
      721 accgcgcagc gccagggcgc gctcggaatc ctcgacccga aaaccggcaa ggtccgccat
      781 attccgctcg gcgagggctc ggcgccgcac ggcgtgatcc aggggccgga cggtgccgcg
      841 tggatcaccg acagcggctt gaacgcgatc gtccggttcg acccgaagag cgagaagcta
      901 acggtgtgga agctgcccga ggacgccggc tatgcgaacc tcaacaccgg cgcgttcgac
      961 ggcgacggga tccactggtt caccggccag aacggcatct acgggcggct cgacccgaag
     1021 accggcgcga tgcgcatctt cagcgacccc gacggccgcg gcccgtacgg gatcgccgcg
     1081 actccgtcgg gcgacgtcta ttatgtgtcg ctggccggaa gccatctcgc caagatcgac
     1141 cgcaagaccg gagcggcggc gatcgtcgag ccgccgaccc cggcgcaggg cgcgcggcgg
     1201 gtgtgggccg acagcaaggg caacctgtgg gtgtccgaat ggaacagcgg caacctcagc
     1261 cgctacacgc cggcgacgaa cacgtggaca atgtggaagc tgccgggcga caagcccaag
     1321 gcctacgcgg tctacgtcga cgagcgcgac atcgtctggg tcagcgactt tgccgccaat
     1381 gcggtgctcg ccttcgaccc gaggacgcag acctggacca aatatccggg cagcggcgag
     1441 ggcgccaacg tccgccagat ccttggggct ccgggcgagg tctatctgcc cgaatcgggc
     1501 accgaccgga tcatggtcgt gcgcaccgcg aggcccggct gaaggcgctg atcgcgttgg
     1561 cgccggcggt cttgctcgcg accgcggctg tcgccggccc gccttcaaaa ccgccgtcga
     1621 agggcgagcg cgcctaccag aaatgctaca gctgccacga gctcgggcgc ggcgcgggtc
     1681 agctcgaagg gccgaccctg cgccggatcg tcggccgccg ggtcgccgcc gagcgcggct
     1741 tcgcttactc gcccgcactg cgccgcttcg cccgccgcca cccgcgctgg acgctgaagc
     1801 tgctcgaccg ctacgtcgcc aatcccgaag cgcttgtccc cggcacgtcg atgaatttcc
     1861 acgggatcag cgatgccgcc gagcgccgcg cgcttgtcga atatttgaat ggagccggcc
     1921 ggagcagtcg ataagcactg gcgcgctcgt taaattattg tttaccggcc catattgccg
     1981 cctggagatt aacgtggttt attgccgccc cggcggcctg gtcaccgagc ctgatcggca
     2041 ggccgggccg ccgccaggaa ccggcgtttt tgccgggcca ccgcagagca ggggtaagga
     2101 atgaaacgca cgattgtaac ggcgctcgcc gccgcttcgc tgttgacgtt gtcggcgtgc
     2161 agccagccgg cgcaggacac gccggccgaa gagcaagcgg ccgcggccga gggcatcgag
     2221 ggcacctgga agagcgacct tgcgtcggtc cagttggagg acgagcccaa cgtctatctc
     2281 atcaaggacg ggcagtatgt ctgcagcacc tgcaatccgc cgctgaccac gccggccgac
     2341 ggtaagttcc atgccgtcgc cgaccgcccc tatttcgaca gcatggcggt cacggtcgtc
     2401 gacgaccgta cggtcaagtt cgagcgcaaa atgggcgatc gcgacgtcgg cagctcgacc
     2461 tggcaactct cggccgacgg caacaccctc aatgtcgagt tcgtcgacaa gacggccgcg
     2521 aaccccgtca ccggcaaggg cagcagcacg cgggtcgccg cggctccggc cggggcccac
     2581 gccacgtccg gctcatggaa agcgaacccg gtgagcaacc tgtcagacga agctctgacc
     2641 ttcagctacg atctcgaagg cgacacgatc cgcaaccggt ccggcgacgg caccagctat
     2701 gaggcgaaga tcggcggccc cgacgcgccg gtccagggcg atccctcggg cctgatggtc
     2761 gcggtcaccc ggccgtcc
//
LOCUS       DASTZO010000280         3264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_963420,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000280 DASTZO010000000
VERSION     DASTZO010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963420"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1417)
                     /gene="serS"
                     /locus_tag="VFU20_07855"
     CDS             complement(158..1417)
                     /gene="serS"
                     /locus_tag="VFU20_07855"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU5286405.1"
                     /translation="MLGMDFVKANREAVGRAIRDKGVELDLDALLALEAEVRGLKTEI
                     EALRAERNAISAQFPTAAAGDKAELGRKAKEAGAKASKLEARLVERETALRAMMLQLP
                     GIPWDGAPVGPDESANTIVRTEGTPPEFGFEPLDHVALIERNDWADLSRIAQVAGSRM
                     YCLKGRLALLEAKLMAWALERIADAGFTPITVPALAREQAFTAQGQFPGHEDETYQLP
                     NDDLWLAGTAEVALTSLHSGEILEADRLPVLYAGYSPCFRREAGSAGRDVRGLLRVHQ
                     FVKVEQYVICEADEAVSAEWHAKLLGLAESLLQALEIPYQVIETSTGDMGLGKYRMND
                     IESWVPSLGRYRETHSCSTLHDWQARRANIRYRGPDGKVRFAHTLNNTALASPRILVP
                     LLENHQAEDGRVRLPQALQQLMGGEFL"
     gene            1473..2444
                     /locus_tag="VFU20_07860"
     CDS             1473..2444
                     /locus_tag="VFU20_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HEU5286406.1"
                     /translation="MPSGLIALLDDVATLTKLAAASVDDVGLAAGKAGTKAAGVVIDD
                     TAVTPRYVTGLSPERELPIIAKITVGSLRNKLLVILPVALLLSAFAPFLIPPVLMIGG
                     AYLAYEATEKILEKLTGDVHDECELLQAETAGELEARQVAGAIRTDFILSAEIMVIAL
                     AELGEESLAMQSGALVAVAIGITFAVYGVVALIVKLDDIGLHMTERGSATARSIGSGL
                     VHGVPYLLTSLSGIGTAAMLWVGGGIILHGMEQMHFEAIPHAVHEGAHALAVAVGVMT
                     PVVEWLANALLASIAGLIVGGVIVGLMHLRPRRHAPAEAAACPEEIV"
     gene            complement(2466..3002)
                     /locus_tag="VFU20_07865"
     CDS             complement(2466..3002)
                     /locus_tag="VFU20_07865"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU5286407.1"
                     /translation="MADPTHPPPHSPHTGEYRTGSDPRTLRDALGCFATGVTVVTCFD
                     EDRRPFGITANSFTSVSLEPPLLLVCVHKMAASAPALTSAGHFAVNVLQTGQQPASIR
                     FSTRDEDRFGNSDWSPGEFGAPVLGNSLSVFECERRAVHDGGDHHILVGQVLKASFDP
                     SLDPLLFFRGRYRRLHFD"
     gene            complement(3043..>3264)
                     /locus_tag="VFU20_07870"
     CDS             complement(3043..>3264)
                     /locus_tag="VFU20_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286408.1"
                     /translation="AVCRNAELARLDRHLGMLYGQSWGQADAARREELLGTRDRFIAR
                     RDSCRSDACIRAAYLGRMREISDIMATKN"
BASE COUNT          468 a         1128 c         1148 g          520 t
ORIGIN      
        1 gtcgtccggt cgttggcgat gaagccgggg atgaccatga ccggctggcc gtctttgggc
       61 ccgcgcgggc ccagatgccc gaaaccgcgc caggaccgcg acgccagagc gccggcttcc
      121 ttgagccgct gcagcgtgcc cggtgcgccg tcctcgctca gaggaattcg cctcccatca
      181 gctgctggag cgcctgcggg agccgcaccc gcccgtcctc ggcctggtgg ttctcgagca
      241 gcgggaccag gatccgcggg gacgcgagcg cggtgttgtt gagcgtgtgg gcgaagcgca
      301 ccttgccgtc gggcccgcgg tagcggatgt tggcgcgccg cgcctgccag tcgtggaggg
      361 tcgagcagct gtgggtctcg cggtagcgcc cgaggctcgg cacccagctc tcgatgtcgt
      421 tcatccgata cttaccgagg cccatgtcgc cggtcgaggt ctcgatcacc tggtagggga
      481 tctcgagcgc ctgcagcagg ctctcggcca ggccgagcag ctttgcgtgc cactcggccg
      541 acaccgcctc gtcggcctcg cagatcacat attgctcgac cttgacgaac tggtggaccc
      601 gaagcagccc gcgcacgtcg cgcccggcgc tgcccgcctc gcgccggaag cagggcgaat
      661 agcccgcgta gaggaccggc agccgatcgg cctcgaggat ctcgcccgaa tgcagcgagg
      721 tcagagcgac ctcggcggtt ccggcgagcc acagatcgtc gttgggcagc tggtaggtct
      781 cgtcctcgtg gcctgggaac tgcccctggg cggtgaacgc ctgctcgcgc gccagcgccg
      841 ggacggtgat cggggtgaag ccggcgtcgg cgatccgctc gagcgcccag gccatcagct
      901 tggcctcgag cagcgccagg cgccccttga ggcagtacat ccgcgagccc gcgacctggg
      961 cgatgcgcga caaatcggcc cagtcgttcc tctcgatcag agcgacatgg tcgagcggct
     1021 cgaagccgaa ctcgggcggg gtcccttcgg tccggacgat ggtgttggcg ctttcgtcgg
     1081 gcccgaccgg ggcgccgtcc cacgggatgc ccggaagctg gagcatcatc gcgcgcagcg
     1141 ccgtctcgcg ttcgaccagc cgcgcctcca gcttgctcgc cttggcgccg gcttccttgg
     1201 ccttgcggcc aagctcggcc ttgtcgcccg ccgccgcggt cgggaattgg gcgctgatcg
     1261 cgttgcgctc ggcccggagc gcctcgatct cggtcttgag cccgcgcacc tccgcctcga
     1321 gcgcgagcag cgcgtcgagg tcgagctcga cccccttgtc gcggatcgct cggccgaccg
     1381 cctcgcggtt ggccttgacg aagtccatcc ccagcatggc cgcggcactg cgcgaattgc
     1441 gccgcgaggg caagttcgcc taagcgcgcc caatgccgtc cggtctcatc gccctgcttg
     1501 acgacgtcgc cacgctgacc aagctcgccg ccgcttcggt cgacgatgtc ggcctcgccg
     1561 ccggcaaggc gggcaccaag gcggccgggg tggtgatcga cgataccgcg gtcaccccgc
     1621 gctacgtaac cggcctgtcg cccgagcgcg agctgccgat catcgccaag atcaccgtcg
     1681 gaagcttgcg caacaaactg ctggtcatcc taccggtggc gctgctgctg tcggccttcg
     1741 cgccgttcct gatcccgccg gtgctgatga tcggcggcgc ctatctcgcc tacgaggcga
     1801 ccgagaaaat cctcgagaag ctgaccggcg acgtccacga cgagtgcgag ctcctgcagg
     1861 ccgaaacggc cggcgagctc gaggcgcgcc aagtggcggg agcgattcgc accgatttca
     1921 tcctgtcggc cgaaatcatg gtgatcgcgc tggccgagct cggcgaggaa tcgctggcga
     1981 tgcagtcggg cgccctggtc gcggtcgcca tcggcatcac cttcgccgtt tacggcgtgg
     2041 tcgccctgat cgtgaagctc gacgacatcg gccttcacat gaccgagcgc ggcagcgcca
     2101 cggcgcgctc gatcggcagc ggcctcgtcc acggggtccc ctatctgctc accagcctgt
     2161 cggggatcgg caccgctgcg atgctgtggg tcggcggcgg catcatcctc cacggaatgg
     2221 agcaaatgca cttcgaggcg atcccccatg ccgtccacga gggagcgcat gctttggccg
     2281 tggcggtcgg ggtgatgact ccggtcgtcg aatggctggc gaatgcgctt ctcgcctcga
     2341 tcgccggcct gatcgtcggc ggggtgatcg tcggcctgat gcacttgcgg cccaggcggc
     2401 acgcgcccgc cgaagccgcc gcctgcccgg aagagatcgt ctaggccgct ttagccgagc
     2461 ctggcctagt cgaagtgcag ccggcggtag cggccgcgga agaacagcaa cgggtcgagg
     2521 ctcgggtcga agctcgcctt cagcacctgg ccgaccagga tgtgatggtc gccgccgtca
     2581 tggaccgccc ggcgctcgca ttcgaacacg ctcagcgaat tgccgagcac cggcgccccg
     2641 aactcgcccg gcgaccagtc gctgttgccg aagcggtcct cgtcgcgggt cgagaaccgg
     2701 atcgaggcgg gctgctggcc cgtctggagc acattgaccg cgaaatggcc ggccgaggtc
     2761 agcgcggggg cgctcgccgc catcttgtgg acgcagacca gaagcagcgg cggctcgagc
     2821 gacaccgagg tgaagctgtt ggcggtgatt ccgaacggcc gccggtcctc gtcgaagcag
     2881 gtgacgacgg tgacgcccgt ggcgaagcag ccgagcgcgt cgcgcagcgt ccgcgggtcg
     2941 gagccggtgc gatattcgcc ggtgtgcggg ctgtgcggcg gtgggtgcgt cggatcggcc
     3001 atccgagccc ttgtagcgac ggcggcaagc gccgccaaag ggctagttct tggtcgccat
     3061 gatgtcgctg atctcgcgca tccggcccaa gtaagcggcg cggatgcagg cgtcggagcg
     3121 gcacgagtcg cggcgggcga tgaagcggtc gcgggtgccg agcaattctt cgcggcgggc
     3181 tgcgtcggcc tgcccccacg actggccgta gagcatcccc aggtggcgat cgagcctcgc
     3241 cagctcggcg ttcctgcaca ccgc
//
LOCUS       DASTZO010000281         3927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1172108,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000281 DASTZO010000000
VERSION     DASTZO010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3927
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172108"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1174
                     /locus_tag="VFU20_07875"
     CDS             <1..1174
                     /locus_tag="VFU20_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5286409.1"
                     /translation="EEAKLKRQIAQATKMQVVGQLAGGVAHDFNNILTAIIGHCDLML
                     MRHTPGDSDYDDIQQVKSNSNRAAGLTRQLLAFSRQQTLRPQVLQLPDVVSEVSHLLK
                     RLLGETVKLVVKHGRDLGPVRADPGQLEQVIVNLAVNARDAMVAQGGGTLTIQTYAVR
                     ADQVAELGSEILPVADYSALSVTDTGCGIPAGVLGKVFEPFFTTKEVGKGTGLGLSTV
                     YGIVKQSGGFIFADSKVGEGTRFVIYLPVHREDSTAGKSRRAAKPKEHELWGTGTVLL
                     VEDEPMVRGVAERALTRHGYTVITAENGEEALEVLARGDEIVLLISDVVMPAMDGPTM
                     VREARKSRPDLKILFMSGYAEEQLRKSIDIDNVAFLPKPFSVQELAEATRRALAAK"
     gene            1214..1570
                     /locus_tag="VFU20_07880"
     CDS             1214..1570
                     /locus_tag="VFU20_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5286410.1"
                     /translation="MAGSRCILIVEDEPLIAMMLEDFLESLGHSISATCDSVDAAMAE
                     VEKGGFDLAILDVNLKGQNVWPVASRLREQGVPFVIASGGHVDPPPPEFASVPLIEKP
                     YTVDRVTPAINAAFAG"
     gene            1551..2399
                     /locus_tag="VFU20_07885"
     CDS             1551..2399
                     /locus_tag="VFU20_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5286411.1"
                     /translation="MPRSPARATLPKLAAAAFVCVAAFLLVMFEFQAQAIFPTHAVAP
                     PGPLPGGAERVTIAAPGGNRLHGVHIPPAAEPAGGRTLVLGFAGNAWNSEDAATYLHD
                     LYPAADVVAFHYRGYRPSTGSPSSAALLEDAPLVHDFAVAKVRSGRTVAVGFSIGSGV
                     AAHLARERPLDGLVLVTPFDSLKAVAAGHYPYLPVGALFRHDMDAAAALKGSRVPTAI
                     IAAERDTIIPPPRTEALRPAIGNLLFDRTIAGAGHNDLYQRPEFRAAMKEALQALGKP
                     GARPGA"
     gene            2571..3836
                     /gene="clpX"
                     /locus_tag="VFU20_07890"
     CDS             2571..3836
                     /gene="clpX"
                     /locus_tag="VFU20_07890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU5286412.1"
                     /translation="MTKLSGGDSKSTLYCSFCGKSQHEVRKLIAGPTVFICDECVELC
                     NDIIREESKSALVKTRDGVPTPAEICKVLDDYVIGQNRAKRVLSVAVHNHYKRLNHGA
                     KTGTDVELAKSNILLIGPTGCGKTLLAQTLARILDVPFTMADATTLTEAGYVGEDVEN
                     IILKLLQASDYNVEKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLMEGT
                     TASVPPQGGRKHPQQEFLQVDTTNILFICGGAFAGLEKIIADRLQGKSIGFGAHVAAP
                     EERQTGEVLKHCEPEDLLKFGLIPEFIGRLPVIATLEDLDEDALVKILVEPKNALVKQ
                     YAKLFDMEDVELDFTDEALGAVARKAIDRKTGARGLRSILEGILLDTMFDLPSMDGVD
                     EVHIDHEVVEGRKEPVRVYAKKGKAGDAA"
BASE COUNT          669 a         1343 c         1284 g          631 t
ORIGIN      
        1 cgaagaagcc aagctcaagc gccagatcgc tcaggcaacc aagatgcagg ttgtcggcca
       61 gctcgccggc ggcgtggcgc acgacttcaa caatatcctt accgcgatca tcggccactg
      121 cgacctgatg ctgatgcggc acactccagg cgacagcgat tatgacgaca tccagcaggt
      181 caagtcgaat tcgaaccgcg ctgcgggcct tactcgacag cttcttgcct tctcgcggca
      241 gcaaaccctg cgcccgcagg tgctccagct tcccgacgtt gtgtcggagg tgtcccattt
      301 gctgaagcgc ctgctcggcg agacggtgaa gctggtcgtc aagcacggcc gcgatttggg
      361 cccggtccgg gccgaccccg gccagctcga gcaggtgatc gtcaacctcg ccgtcaacgc
      421 ccgcgacgcg atggtcgcgc agggcggcgg cacgctgacc atccagacct acgcggttcg
      481 cgccgaccag gtcgccgagc tcggctcgga gatccttccg gtcgccgact atagcgcgct
      541 gtcagtcacc gacaccggct gcggcattcc cgccggagtg ctcggcaagg tgttcgagcc
      601 attcttcacc accaaggaag tcggcaaggg caccggcctc ggcctttcga ccgtctacgg
      661 catcgtcaag cagtcgggcg gcttcatctt cgccgattcc aaggtcgggg agggcacccg
      721 cttcgtcatc tatttgccgg tccaccgcga ggattcgacc gccggcaagt cgcgccgcgc
      781 cgccaagccc aaggagcacg agctgtgggg gaccggcacc gtcctcctgg tcgaggacga
      841 gccgatggtg cgcggggtcg ccgagcgggc gctcacccgc cacggctata cggtgatcac
      901 cgccgagaac ggcgaggaag cgctcgaggt gctcgcccga ggcgatgaga tcgtccttct
      961 gatcagcgac gtggtgatgc cggcgatgga cgggccgacc atggtccgcg aggcgcgcaa
     1021 gagccgcccc gacctcaaga tcctgttcat gtcgggctat gccgaggagc aattgcgcaa
     1081 gtcgatcgac atcgacaatg tcgccttcct gcccaaaccc ttttcggttc aggagcttgc
     1141 cgaggccacc cggcgcgctc tggcggccaa ataatcgttg ctcttgcgtg ccggtcgatt
     1201 attcgatgcg cttatggcgg ggtcgcgttg catattgatc gtcgaggacg aaccgctgat
     1261 cgcgatgatg ctcgaggatt tcctcgaatc gctcggccat tcgatctccg cgacctgcga
     1321 cagcgtcgac gcggcgatgg ccgaggtcga gaagggcggg ttcgacctcg ccatcctcga
     1381 cgtcaatctg aagggccaga acgtgtggcc ggtcgccagc cggctgcgcg agcagggcgt
     1441 cccgttcgtc atcgccagcg gcggccacgt cgacccgccg ccgcccgagt tcgcgtcggt
     1501 gccgctgatc gagaagccct ataccgtcga ccgggtgacc ccggcgatca atgccgcgtt
     1561 cgccggctag ggcgaccctt cccaagctcg ccgctgcggc ctttgtctgc gttgccgcgt
     1621 tcctgctggt gatgttcgaa ttccaggccc aggcgatttt cccgacgcac gcggtcgcgc
     1681 cgccgggtcc gctgccgggc ggcgccgagc gggtcaccat cgccgcgccc ggcggcaacc
     1741 ggcttcacgg cgtgcacatt ccgccggccg cggagccggc gggcgggcga acgctggtcc
     1801 tcggcttcgc cggcaacgcc tggaacagcg aggacgcggc gacctatctg cacgatctct
     1861 acccggcggc ggacgtggtc gccttccact atcgcggcta ccggccgtcg accggctcgc
     1921 cgtcgtcggc ggcgctgctc gaggatgcgc ccctggtcca cgatttcgcg gtcgcgaagg
     1981 ttcggtccgg gcggaccgtc gccgtcggct tcagcatcgg cagcggggtc gccgcgcacc
     2041 tcgcccgcga gcgtccgctc gacgggctgg tcctggtcac tccgttcgat tccctgaaag
     2101 cggtcgccgc cggccattac ccgtaccttc cggtcggggc cctgttccgt cacgacatgg
     2161 acgccgccgc cgccctgaag ggcagccggg tgccgaccgc gatcatcgcc gccgagcgcg
     2221 acacgatcat cccgccgccc cgaaccgagg cgctgcgccc ggcgatcggc aacctgctgt
     2281 tcgaccggac gatcgcgggc gccggccaca acgacctcta ccagcggccc gaattccggg
     2341 cggcgatgaa agaggcgttg caggcgctcg gcaagcctgg cgcccggccc ggcgcctagc
     2401 cgcgggggcg aggccttgcc gcggcccggc cgccaagcca tattcctgaa atggaacagc
     2461 aatcgaggcg gcgggcgatc gttaagattc ccgagagttt gattgtgcaa taaagatggc
     2521 tagcatggcg accgacggaa gggcgtcaaa ggccggacga taggattgga atgaccaagc
     2581 tttcaggcgg cgacagcaag agcaccctct actgctcgtt ctgcggcaag tcgcagcatg
     2641 aggtcaggaa gctgatcgcc gggccgaccg tgttcatctg cgacgaatgc gtcgagctgt
     2701 gcaacgacat catccgcgag gaatcgaagt cggcgctggt caagacccgc gacggagtcc
     2761 cgacgccggc cgagatctgc aaggtgctcg acgattatgt gatcggccag aaccgcgcca
     2821 agcgggtgct ctcggtcgcc gtccacaacc attacaagcg cctcaaccac ggcgccaaga
     2881 ccggcaccga cgtcgagctc gccaagtcga acatcctgct gatcggcccg accggctgcg
     2941 gcaagacgct gctcgcccag accctcgcgc ggatcctcga cgtgccgttc accatggccg
     3001 acgcgacgac cctgaccgag gccggctacg tcggcgagga cgtcgagaac atcatcctga
     3061 agttgctcca ggcgtccgac tacaatgtcg agaaggcgca gcgcgggatc gtctacatcg
     3121 acgagatcga caagatcagc cgcaagtccg acaatccgtc gatcacccgc gacgtgtcgg
     3181 gcgagggcgt ccagcaggcg cttctgaagc tgatggaggg gaccaccgcc tcggtcccgc
     3241 cgcagggcgg ccgcaagcac ccgcagcagg agttcctgca ggtcgacacg accaacatcc
     3301 tgttcatctg cggcggtgcg ttcgccggcc tcgagaagat catcgccgac cggctgcagg
     3361 gcaagtcgat cggcttcggc gcccatgtcg ccgctcccga ggagcgccag accggcgagg
     3421 tgctcaagca ttgcgagccc gaggatctgc tcaagttcgg gctgatcccc gagttcatcg
     3481 gccggctgcc ggtgatcgct accctcgagg acctcgacga ggacgccctg gtcaagatcc
     3541 tggtcgagcc gaagaacgcc ctggtcaagc aatatgcgaa gctgttcgac atggaagatg
     3601 tcgagctcga cttcaccgac gaggcgctgg gagcggtcgc gcgcaaggcg atcgaccgca
     3661 agaccggcgc ccgcgggctg cgctcgatcc tcgaggggat cctgctcgac acgatgttcg
     3721 acctgccgtc gatggacggg gtcgacgagg tccatatcga ccatgaggtc gtcgagggcc
     3781 gcaaggagcc ggtccgggtc tacgccaaga aaggcaaggc cggcgacgcc gcctgacctc
     3841 gcgtttcctg atcgatgatg ggcgacgttc gtgcgcggct tgccgggtgg cggccgccga
     3901 tcacctttct cccccccgcg cttcgcg
//
LOCUS       DASTZO010000282        14777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_23506,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000282 DASTZO010000000
VERSION     DASTZO010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 14777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14777
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23506"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="VFU20_07895"
     CDS             <1..287
                     /locus_tag="VFU20_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019832950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5286413.1"
                     /translation="EARRPYRGYARNIGLAFQIADDLIDHSGDEAAAGKRTGKDAPAG
                     KATFVSLLGEDRARQQAHFLVEQAIEHLASHGSEADLLRSIARFAIERDH"
     gene            291..782
                     /gene="coaD"
                     /locus_tag="VFU20_07900"
     CDS             291..782
                     /gene="coaD"
                     /locus_tag="VFU20_07900"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU5286414.1"
                     /translation="MATRVGVYPGTFDPITLGHLDIVRRGAHLVDRLVIGVTTNPSKE
                     PMFSLPERLEMVRREVAGIPGDISVVEFDSLLMDFAVREGASLILRGLRAVSDFEYEY
                     QMAGMNQQLNDEVETVFLMADVSLQPIASRLVKEIARYGGAIDKFVTPAVAADVAAHL
                     GRR"
     gene            869..1534
                     /locus_tag="VFU20_07905"
     CDS             869..1534
                     /locus_tag="VFU20_07905"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008994834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5286415.1"
                     /translation="MFAAAALASAESSAQVPAAGYAPILPPPAVAAEPANRLTLELSN
                     GGRVVIQLRPDAAPAHVERIQTLVRRGFYNGVAFHRVIPGFMAQGGDPKGTGAGGSEL
                     PDLKAEFTTIPYMRGIVAAARTDDPNSANSQFFIMFSPNVELWGKYTVFGRVIAGMDA
                     VDRIAVGEPPADPTRIVRASLGDTVAVQAPAPAPAPAAPAPAPAAPAPADQATVEAPP
                     AAD"
     gene            1534..2571
                     /gene="queA"
                     /locus_tag="VFU20_07910"
     CDS             1534..2571
                     /gene="queA"
                     /locus_tag="VFU20_07910"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU5286416.1"
                     /translation="MRVDLFDFELPPDRIALRPARPRDSARLLQVEGADISDHNVLDL
                     PALLRSGDVLVFNDTKVIPAQLEGRRGDARIGATLHKREGPRSWWSFVRNARRLHVGD
                     RVEFAAGVSASAVERGEEGAFLLQFHGDEPVDQLIDRAGHMPLPPYIASRRPPDEADR
                     ADYQTMFARAEGAVAAPTAALHFTPRLLDALDERGIARETLTLHVGAGTFLPVKADMV
                     AEHRMHAEWGRIDAQTAQRLNRARAAGGRLIAVGTTSLRLIESAAGEDGEIRPFEGDT
                     EIFITPGYRFRAVDGLVTNFHLPRSTLFMLVSALMGLDVMKSAYAHAVERGYRFYSYG
                     DASLLLPAHAR"
     gene            2699..3793
                     /locus_tag="VFU20_07915"
     CDS             2699..3793
                     /locus_tag="VFU20_07915"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HEU5286417.1"
                     /translation="MNAPQMGFMPDNEAERIAAVQRYQILDTPPDGIFDRVTAVAARR
                     FGTPIAIISIVDHDRIWFKSHHGLDVEEIGRDPGLCASAILSSDVHLLLDASTDPRSL
                     ANPLVAGDFGLRFYAGVPLRTSDGFNLGTLCVIDKEPRTVEQDQIDDLTDLASLVVDQ
                     LELRLAARRAVAQSELMAREIDHRIMNSLQFVAGLLSMQSRSAGNDEVASQLTQAATR
                     VAAVARVHRNFYSGDSDEVSCLTFLTRLCADLSEICGVSVEVSGDEGTVPTTMIQPIG
                     LLTNELVTNAAKHGAGDVTVEYRIAADRHGLSVCDEGTGVPADFEPAMSKGLGMKVVT
                     LIAKQLGGALEVLPNPAGRGACFRVSFDRR"
     gene            3875..5020
                     /gene="tgt"
                     /locus_tag="VFU20_07920"
     CDS             3875..5020
                     /gene="tgt"
                     /locus_tag="VFU20_07920"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU5286418.1"
                     /translation="MTRFSFTIAATDGAARTGAIALERGVVRTPAFMPLGTAGTVKAM
                     KPGDVAASGADMILGNTYHLMLRPGAERVARLGGLHCFMGWERPILTDSGGYQVMSLS
                     DLTRVSEEGVSFKSHLDGSAHLLTPERSIEVQRLLGADVVMAFDQLVPTTSQRADQVA
                     AMERSMRWAKRSRDAFDAGGEHSENSALFGIQQGALDEGLRKASADALTGIGFDGYAV
                     GGLAVGEGQQAMLGCLDFAAGMLPADAPRYLMGVGKPDDIVEAVRRGIDMFDCALPTR
                     SGRTGQAFTADGALNLRNARFAEDREPIEPGCPCPACGKFERAYVHHLVKSGEILGAM
                     LMTEHNLWFYQRLMQELREAIAGRRFAAHASAFLERYRKNSPSPNSA"
     gene            complement(5073..6500)
                     /locus_tag="VFU20_07925"
     CDS             complement(5073..6500)
                     /locus_tag="VFU20_07925"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5286419.1"
                     /translation="MSFADLGLSDELLRAVADSGYGEPTPIQKGVIPSVLMGKDIVGI
                     AQTGTGKTAGFVLPMLDILHHGRSRARMPRSMILEPTRELAAQVAENFEKYGKYHKLS
                     MALLIGGVQMGDQVKALEKGVDVLIATPGRLMDLFGRGKILLNGCELLVIDEADRMLD
                     MGFIPDIEEICSKLPKSRQTLLFSATMPPPIQKLAAKFLNDPKRVEATRPATANVNIE
                     QRLVEVRPDKKKDKLRELLRATEFKNAIVFCNRKTTVRELASSLKRSGFAVGQIHGDM
                     EQRDRIAEFDRFKAGEINILVASDVAARGLDVKGVSHVINFDVPWQPDDYIHRIGRTG
                     RAGMTGIAISLATPADAEQVAAIEKLTGTRIARVGAVREGAAPPEPAGDRRPSEASEP
                     REPRESRRPRRERKPRREVAEPKAAEPKPTEPEPTEPKPAEPPAKAKEERKPRPPRRE
                     PQPEAEDIDEGWNGPMPSFLSIRAD"
     gene            complement(6609..7055)
                     /locus_tag="VFU20_07930"
     CDS             complement(6609..7055)
                     /locus_tag="VFU20_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286420.1"
                     /translation="MTVSSFLALLPLLLGVAATQDQAPGDEPQPAVSRLVVERQWIVR
                     VPVRPHSAPRRFEWNEGKRVKCFPTNAIRGALLSGADHVDFLLRPRQRVRATFDGNCP
                     ALDFYGGFYLKTDDDRVCARRDFVHSRMGGSCRIERFRQLVPRIKD"
     gene            7275..8066
                     /locus_tag="VFU20_07935"
     CDS             7275..8066
                     /locus_tag="VFU20_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HEU5286421.1"
                     /translation="MATAAPNQTLPLFYSSLEALNVTQHGKMKIRPVEKVPTIATTHA
                     IPATVDEFALLQRYYPIVFSIGDNPVPLVLMGLNEGVNVFLNEDGKLHDPTIYVPAYM
                     RRYPFLLARLRPDSDDLSLCFDPTSGAVGDFEDGEPLFDGDQPSQATKAILEFCEQFE
                     TAGQRTAAFIEDLKKSGLLMDGEVAIQPEGAEQPFVYRGFQMVDEEKLRELRGDELRK
                     MNQSGMLALIYAHLFSLPQIREVFSRQVMQGKGPMAVPQRATVEA"
     gene            complement(8233..9096)
                     /locus_tag="VFU20_07940"
     CDS             complement(8233..9096)
                     /locus_tag="VFU20_07940"
                     /inference="COORDINATES: protein motif:HMM:NF016873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HEU5286422.1"
                     /translation="MSLPESPIAPLPAPIVHPPRAGLPVLLSVPHSGRDYPDWLVAMA
                     SGGRRALAALEDPLVDRLAWRAVERGIAAVIARAPRAAIDCNRGEDEVDPSVIEGGPG
                     RGKVSARARGGLGIVPGRTQSHGYLWRRPISPAQLEDRLDQAHRPYHRALEQQLGKLA
                     GRFGCALLLDCHSMPPPARGVPPIVFGDRHGRSAAPWLSAEAVRIAGAAGFAAGLNDP
                     FAGGHIVERHGAPERGIHALQVEIDRRSYLNERLDSPGPGFDSVASLLEALALGLGDR
                     LMGRQLATAAE"
     gene            9196..9573
                     /locus_tag="VFU20_07945"
     CDS             9196..9573
                     /locus_tag="VFU20_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5286423.1"
                     /translation="MARILLAEDDTSMREYLQRALQRVGYEVEAVGCGTEAMPLLEAG
                     RFDLLLTDIVMPEMDGIELAQKASVIDPSIRVMFITGFAAVALQGGRTAPEAKLLSKP
                     FHLKDLVAEVDRIFQTEDQHGRL"
     gene            9643..9717
                     /locus_tag="VFU20_07950"
     tRNA            9643..9717
                     /locus_tag="VFU20_07950"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9675..9677,aa:Val,seq:gac)
     gene            9839..10297
                     /locus_tag="VFU20_07955"
     CDS             9839..10297
                     /locus_tag="VFU20_07955"
                     /inference="COORDINATES: protein motif:HMM:NF021536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HEU5286424.1"
                     /translation="MSANGLEVFDKTLETTHIWLKEIMADIGPDRQVAWKVLSVVLHK
                     LRDRLPLGLAAHLGSQLPLLVRGVYYDQFEPGKLPTTCNSREEFVAEVAEWLTDTRPV
                     DPDLAVRSVFKVLSNHVSEGQVAKVREALPRGLRQSWIDAEAREPADSDA"
     gene            complement(10329..11162)
                     /locus_tag="VFU20_07960"
     CDS             complement(10329..11162)
                     /locus_tag="VFU20_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5286425.1"
                     /translation="MLSRPLFGLLLAALALPACAPVGPASAQVSPEALAVAVASPSRT
                     PANLARDRYRHPAETLAFFGVRPSDTVVELWPGGGWYTEILAPYLAAGGGRLYAAAPE
                     WGMTGIAKLKAANPALYGPVLTADFPVFDAEDTPVPAGSADVVLTFRNVHNWRMGYRR
                     DDKQDYSAEAFRQIHAMLKPGGTLGIVDHRLPEDASDERERSSGYIKVSTVRRLAEQA
                     GFRLAAASEINANPKDTADWPDGVWTLPPSLALKDKDREKYLAIGESDRMTLKFVKPK
                     R"
     gene            complement(11240..12775)
                     /locus_tag="VFU20_07965"
     CDS             complement(11240..12775)
                     /locus_tag="VFU20_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HEU5286426.1"
                     /translation="MSAFGKRGGMSGGGQRPSFGVARPMKSPGGKAQAVPSGGDQFPP
                     LEEIAPQVESLPDDIGGAGDGGSMGAMDRLTARQNQSGEQGSSKAEGFEASVHRIKEQ
                     VLPRLLERVDPEAAATLSKDELAEEFRPIIGEVLAELKITLNRREQFALEKVLVDELL
                     GLGPLEELLADPAISDIMVNGPDQCFIERKGKLELAQIQFRDEEHLFQIAQRIVNKVG
                     RRVDQTTPLADARLQDGSRVNVIIPPLSLRGTAISIRKFSEKPITLDMMTGFGSMSDK
                     MATLLKIAGASRMNVIISGGTGSGKTTMLNALSKMIDPGERVITIEDAAELRLQQPHW
                     LPLETRPANLEGQGAITIRDLVINALRMRPDRIILGEIRGPECFDLLAAMNTGHDGSM
                     ATLHSNSPRECLARMENMVMMSDIKIPKEAISRQIADSVDLIVQVKRLRDGSRRTTNI
                     TEVIGMEGDVIVTQELFKFEYLDESPDGKILGEYRSMGLRPYTLEKAKQFGFDQPYLE
                     ACL"
     gene            complement(12981..13238)
                     /locus_tag="VFU20_07970"
     CDS             complement(12981..13238)
                     /locus_tag="VFU20_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEU5286427.1"
                     /translation="MADLHFELVTPEKLVMSEDVHMVVVPGTEGEFGVLAGHAPYMST
                     LRDGELAIYRTAGAQPEKIAISGGFAEVSERGLTVLAESTG"
     gene            complement(13242..14759)
                     /gene="atpD"
                     /locus_tag="VFU20_07975"
     CDS             complement(13242..14759)
                     /gene="atpD"
                     /locus_tag="VFU20_07975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU5286428.1"
                     /translation="MATAAEPKTRTKKAATKSAPAPKSPTTGGNIVGKVAQVIGAVVD
                     VAFEGELPPILAALETDNHGQRLVLEVAQHLGENIVRTIAMDSTDGLTRGQQVTATGS
                     QIQVPVGPKTLGRIMNVVGEPIDERGPIGSDKFASIHAEAPKFVDQSTEAAILVTGIK
                     VIDLLAPYARGGKIGLFGGAGVGKTVLIQELINNIAKGHGGTSVFAGVGERTREGNDL
                     YHEFLDAGVIAKDKDGNAISEGSKVALVYGQMNEPPGARARVALSGLAIAEYFRDAEG
                     QDVLFFIDNIFRFTQAGSEVSALLGRIPSAVGYQPTLATDMGSLQERITSTNKGSITS
                     VQAIYVPADDLTDPAPATSFAHLDATTVLSRAISELGIYPAVDPLDSTSRVLEPRTVG
                     QEHYDTARAVQETLQRYKSLQDIIAILGMDELSVEDKLVVSRARKIQRFLSQPFHVAE
                     VFTGIPGKFVQLEDTIRSFKAVVDGEYDHLPEAAFYMVGGIEEAVAKGQRLAEDA"
BASE COUNT         2251 a         4933 c         5186 g         2407 t
ORIGIN      
        1 ttgaagcgcg caggccctat cgtggctatg cgcgcaacat cggcctcgcc ttccagatcg
       61 ccgacgatct gatcgaccat agcggcgacg aggcggcggc cggcaagcgc accggcaagg
      121 acgcgcccgc cggcaaggcg accttcgtgt cgctgctcgg cgaggaccgc gcgcgccagc
      181 aggcgcattt cctggtcgag caggcgatcg agcacctcgc gagccacggc agcgaagcgg
      241 acctgctccg ctcgatcgcc cgcttcgcca tagaacggga tcattgagcc atggcgacac
      301 gcgtgggagt ctatcccggc accttcgacc cgatcacgct gggccatctc gacatcgtcc
      361 gccgcggcgc gcacctggtc gaccggctgg tgatcggggt caccaccaac ccgtccaagg
      421 agccgatgtt ctcgcttccc gagcggctcg agatggtccg ccgcgaggtc gccggaatcc
      481 ccggcgacat cagcgtcgtc gagttcgatt cgctgctgat ggacttcgcc gtgcgcgagg
      541 gcgcgtcgct gatcctgcgc ggcctgaggg cggtgtcgga tttcgaatat gagtatcaga
      601 tggcgggcat gaaccagcag ctgaacgacg aggtcgagac cgtcttcctg atggccgacg
      661 tctcgctgca gccgatcgcg tcgcggctgg tcaaggagat cgcgcgctac ggcggggcga
      721 tcgacaagtt cgtgaccccg gcggtggcgg ccgacgtcgc cgcgcatctc gggcgccgct
      781 agggctggcg cgcgcgggcg ctccgcgcta gtggcggagc cgggctccgg ggagtgggtg
      841 agaaatgcgt aaagcgttga cggcattgat gtttgcggct gccgcactgg cttcggccga
      901 gtcttcggcg caggtcccgg cggccggcta tgcgccgatc ctgccgccgc ccgcggtcgc
      961 cgccgaaccg gccaaccggc tgacgctcga gctgtcgaac ggcggccggg tggtgatcca
     1021 gctccgcccc gacgccgccc cggcgcacgt cgagcgaatc cagaccctcg tccgccgcgg
     1081 cttctacaac ggcgtcgcct tccaccgcgt gatccccggc ttcatggccc agggcggcga
     1141 ccccaagggc accggcgcgg gcggctccga gcttcccgac ctcaaggccg agttcaccac
     1201 catcccctac atgcgcggga tcgtcgccgc cgcgcgcacc gacgacccca attcggccaa
     1261 cagccagttc ttcatcatgt tctcgcccaa cgtcgagctg tggggcaagt acacggtgtt
     1321 cgggcgggtg atcgcgggca tggacgcggt cgaccggatc gcggtcggcg agccgccggc
     1381 cgacccgacg cggatcgtcc gggcgagcct cggcgacacg gttgcggttc aggcgcccgc
     1441 gccggccccg gccccggccg cgccggcccc ggccccggcc gcgccggccc ccgccgacca
     1501 ggcgacggtg gaggccccgc cggcggccga ctgatgcgcg tcgacctgtt cgatttcgag
     1561 cttccacccg atcgcatcgc cttgcggccc gcccggccgc gggacagcgc ccggctgctg
     1621 caggtcgagg gcgcggacat ctcggaccac aatgtgctcg acctgccggc gctgctgcgg
     1681 agcggcgacg tgctggtgtt caacgacacc aaggtgatcc cggcgcagct cgagggacgg
     1741 cgcggcgacg cgcggatcgg cgccacgctg cacaagcgcg aggggccgcg cagctggtgg
     1801 tcgttcgtcc gcaacgcccg gcggctgcac gtcggcgacc gggtcgagtt cgcggccgga
     1861 gtctcggcct ccgccgtcga gagaggggag gagggcgcgt tcctgctcca gttccacggc
     1921 gacgagccgg tcgaccagct gatcgaccgc gccggccaca tgcccctgcc gccctacatc
     1981 gcctcgcgcc ggccgcccga cgaggccgac cgcgccgatt accagaccat gttcgcgcga
     2041 gctgagggag cggtcgccgc gccgaccgcg gcgctgcatt tcacgccgcg gctgctcgac
     2101 gcgctcgacg agcgcggaat cgcccgcgaa acgctgaccc tgcacgtcgg cgcgggcacc
     2161 ttcctgccgg tcaaggccga tatggtcgcc gagcaccgga tgcatgcgga gtgggggcgg
     2221 atcgacgcgc aaacggcgca gcggctcaac cgggcgcggg cggcgggcgg gcggctgatc
     2281 gcggtcggaa cgacctcgct tcggctgatc gaaagcgcgg caggcgagga cggcgaaatc
     2341 cggccgttcg aaggcgacac ggagattttc atcacgcccg gctaccgctt cagggcggtc
     2401 gacgggctgg tcaccaattt ccatttgccg cgctcgaccc tgttcatgct ggtcagcgcg
     2461 ctgatggggc tcgacgtcat gaagtcggct tacgcccacg cggtcgagcg cggctaccgc
     2521 ttctacagct acggcgacgc gagcctgctg ctgcctgccc acgcgcgctg aaccgggcgg
     2581 ttgccttgcg cgcgcccagc agggcttctt tcgagtcggg aaagcggcta accggatgca
     2641 atcgtaacaa ttttcctagt tctcatttcc catccgaccg tcttgcaggg gcccaccgat
     2701 gaacgcgccg caaatggggt tcatgcccga caacgaagcc gagcgcatcg ccgcggtcca
     2761 gcgctaccag attctcgaca ctccgccgga cgggattttc gaccgggtga cggcggtggc
     2821 cgcgcgccgc ttcgggaccc caatcgcgat catcagcatc gtcgaccatg accgcatctg
     2881 gttcaagtcc caccacggtc tcgacgtcga ggagatcggc cgcgaccccg ggctgtgcgc
     2941 gtcggcgatc ctgtcgtccg atgtccatct cctgctcgac gcgagcaccg atccgcgctc
     3001 gctcgccaat ccgctggtcg ccggcgactt tgggctgcgc ttctatgccg gagtcccctt
     3061 gcggacctcc gacgggttca acctcgggac tctatgcgtg atcgacaagg aaccgcgaac
     3121 ggtcgagcag gaccagatcg acgatctgac cgacctcgcg tccctggtcg tcgaccagct
     3181 cgagttgcgg ctcgccgccc gccgggccgt cgctcagtcc gagctgatgg cccgcgagat
     3241 cgaccaccgg atcatgaaca gcctgcagtt cgtggccggg ctgctgtcga tgcaaagccg
     3301 ctcggctggc aatgacgagg tcgcctcgca gctgacccag gccgcgaccc gggtggccgc
     3361 agtcgcgcgg gtccaccgca acttctattc gggcgacagc gacgaagttt cgtgtctcac
     3421 cttcctcact cgcctgtgcg ccgacctttc ggagatttgc ggagtgtccg tcgaggtcag
     3481 cggcgacgag ggcacagtcc cgacgacgat gatccagccg atcgggttgc tgaccaacga
     3541 gcttgtcacc aatgccgcca agcatggcgc cggcgatgtc acggttgagt acaggatcgc
     3601 ggcggaccgc cacgggctga gcgtctgcga cgagggcacg ggggttcccg ccgatttcga
     3661 gccggcgatg tccaaaggcc tcgggatgaa ggtcgtcacg ctgatcgcaa aacagctcgg
     3721 cggagcgctt gaggtgctcc ccaacccggc cggccgaggc gcctgcttcc gggtctcttt
     3781 cgaccgccga taagcaaagg ctcgtaaatt cgctggccgg gcgggagcgc gcgtggctcg
     3841 acttggctcg gcgcgagcgg ttagggcggc gcggatgacc cgtttttcgt tcaccatcgc
     3901 cgcgaccgac ggagccgcgc gcaccggcgc gatcgcgctg gagcgcgggg tcgtgcggac
     3961 tccggcgttc atgcccttgg gcaccgcggg cacggtcaag gcgatgaagc cgggcgacgt
     4021 cgccgcctcg ggcgccgaca tgatcctcgg caacacctat catctgatgc tccgcccggg
     4081 cgccgagcgg gtcgctcggc tgggcgggct gcactgcttc atgggctggg agcggccgat
     4141 cctgaccgac agcggcggct accaggtgat gagcctgtcg gacctgaccc gggtcagcga
     4201 ggagggggtg agcttcaaga gccacctcga cggctcggcg catctgctga ccccagaacg
     4261 gtcgatcgag gtgcagcgat tgctcggcgc cgacgtggtc atggcgttcg accaactggt
     4321 gccgacgacg tcccagcgcg ccgaccaggt cgcggcgatg gagcgctcga tgcggtgggc
     4381 gaaaaggtcg cgcgacgcgt tcgacgcggg cggcgagcat tcggagaatt cagcgctgtt
     4441 cggaatccag cagggggcgc tcgacgaggg gctccgcaag gcctcggcgg acgcgctgac
     4501 cgggatcggg ttcgacggct atgcggtcgg cgggctggcg gtcggcgagg ggcagcaggc
     4561 gatgctcggc tgcctcgact tcgccgccgg gatgctgccg gccgacgcgc cgcgatacct
     4621 gatgggggtc ggcaagcccg acgacatcgt cgaggcggtg cgccgcggca tcgacatgtt
     4681 cgattgcgcg cttccgacgc gctcggggcg caccggccag gcgtttaccg ccgacggcgc
     4741 gctcaacctc cgcaacgcgc gcttcgccga ggaccgcgag ccgatcgagc ccggctgccc
     4801 gtgtccggcg tgcggcaagt tcgagcgcgc ctacgtccac cacctggtca agtcgggcga
     4861 gatcctgggc gcgatgctga tgaccgagca caatctgtgg ttctaccagc ggctgatgca
     4921 ggagctgagg gaggcgatcg cgggccgtcg cttcgccgct cacgcgagcg cgttcctgga
     4981 gcgttaccgg aaaaattccc cttcacccaa ctccgcctaa gctcgttcct cgccaaggct
     5041 ccgtaaccct ctccctcaag gggagagggg gcttagtcgg cgcggatcga gaggaaggac
     5101 ggcatcgggc cgttccagcc ttcgtcgatg tcctcggcct cgggctgcgg ctcgcggcgc
     5161 ggcgggcgcg gcttgcgctc ttccttcgcc ttggccggcg gctcggcggg cttcggttcg
     5221 gtgggctctg gctcggtggg ctttggctcg gcggcctttg gctcggctac ctcacggcgc
     5281 ggcttgcgct cgcggcgggg gcggcgcgat tcgcgcggtt cgcggggctc gcttgcctcg
     5341 ctcggtcggc gatcgccggc gggctcgggc ggagcggccc cctcccgtac cgcgcccacc
     5401 cgcgcgatcc gggtgccggt cagcttctcg atcgccgcaa cctgctcggc gtcggccggg
     5461 gtggcgagcg aaattgcgat cccggtcatg cccgcccggc cggtgcggcc gatccggtgg
     5521 atgtagtcgt cgggctgcca cggaacgtcg aaattgatca catggctgac gcctttgacg
     5581 tcgaggccgc gcgccgcgac gtcggacgcg accaggatgt tgatctcgcc cgccttgaag
     5641 cggtcgaact cggcgatgcg gtcccgctgc tccatgtcgc cgtggatctg gcccaccgcg
     5701 aagcccgagc gcttgaggct cgaggcgagc tcgcgcaccg tcgtcttgcg gttgcagaac
     5761 acgatcgcgt tcttgaattc ggtggcgcgc aggagctcgc gcaacttgtc ctttttcttg
     5821 tccggccgga cctcgaccag gcgctgctcg atgttgacgt tggcggtcgc cggacgggtc
     5881 gcctcgaccc gcttgggatc gttgaggaac ttggcggcga gcttctggat cggcggcggc
     5941 atggtcgccg agaacagaag ggtctggcgc gatttcggaa gcttcgagca gatttcctcg
     6001 atgtcgggga tgaagcccat gtcgagcatc cggtcggcct cgtcgatgac cagcagctcg
     6061 cagccgttca agaggatctt gccgcggccg aacaggtcca tcaggcgtcc cggagtcgca
     6121 atcaggacat ccacgccttt ctccagcgcc ttgacctggt cgcccatctg aacgccgccg
     6181 atcagcagcg ccatcgagag cttgtggtac ttgccgtatt tctcgaaatt ctcggcgacc
     6241 tgggcggcga gctcgcgggt cggctcgagg atcatcgagc gcggcattcg ggctcggctg
     6301 cggccatggt gcaggatgtc gagcatcggc aggacgaagc cggcggtctt gccggtgccg
     6361 gtctgggcga tgccgacgat gtccttgccc atcaggaccg acgggatgac ccccttctgg
     6421 atcggggtgg gctcgccata gccgctgtcg gcgaccgcgc gcagcaattc gtcggaaagg
     6481 ccgagatcgg caaaagacat tcaaaaatcc ggaaaaatga ggtggcgagc gccctcgcta
     6541 agcggccgca gctgccgccg gggctaacgc tgtcgcggcg aaattgtcaa ggaaagcccc
     6601 gtctttgatc agtccttgat cctgggcacg agctgccgga agcgctcgat tcggcagctc
     6661 ccgcccatcc gcgaatggac gaaatcgcgc cgcgcgcaga cgcggtcgtc gtcggtcttc
     6721 aggtagaagc cgccgtagaa gtcgagcgcc gggcaattgc cgtcgaacgt cgcccggacc
     6781 cgctgccgcg ggcgaagcag gaagtcgaca tggtcggcgc cggacagcaa ggcgccgcgg
     6841 atggcgttgg tcgggaagca tttgacgcgc ttgccctcgt tccactcgaa ccgccgcggc
     6901 gccgagtgcg gccggaccgg gacccggacg atccactggc gctcgaccac cagccggctg
     6961 accgccggct gcggctcgtc gcctggggct tggtcctggg tggcggcgac gccgagcagc
     7021 agcggaagca gcgccaggaa gctggacacg gtcattcggc ggtcaccatc atcggccgag
     7081 gcttcgcagc cgagggttga atggtcaatg aattgaaggc tcagccgcgg cccggggccg
     7141 ggggccgaga gtgcggcctt gcctaaacag gcggctcgga atagaggctc gaccgcaaat
     7201 cttgccggct cgatgacggc gctcgggagc gcctcaaccc cggatacgcc tgataccctt
     7261 gggagatcga tcgaatggcg actgccgcgc cgaaccagac cctgccgtta ttctattcct
     7321 cgctcgaggc gctcaacgtc acccagcacg ggaagatgaa gatccggccg gtcgagaaag
     7381 tgccgacgat cgccacgacc cacgcgatcc cggcgacggt cgacgaattc gccctgctgc
     7441 agcggtacta tccgatcgtc ttctcgatcg gcgacaaccc ggtgccgctg gtgctgatgg
     7501 gcctcaacga gggcgtcaac gtgttcctca acgaggacgg caagctgcac gatccgacga
     7561 tctacgtgcc ggcctatatg cgccgctacc cgttcctgct ggcgcggctc cggccggaca
     7621 gcgacgatct gtcgctgtgc ttcgacccga cgtcgggcgc ggtcggcgac ttcgaggacg
     7681 gcgagcccct gttcgacggc gaccagccga gccaggcgac caaggcgatc ctcgagttct
     7741 gcgagcaatt cgagacggcc ggccagcgca ccgcggcctt catcgaggat ttgaagaagt
     7801 cggggctgct gatggacggc gaggtcgcca tccagcccga gggcgccgag cagccgttcg
     7861 tttatcgcgg cttccagatg gtcgacgagg aaaagttgcg cgagcttcgc ggcgacgagc
     7921 tgaggaagat gaaccagagc gggatgctgg cgctgatcta cgcgcatctg ttctcgctgc
     7981 cgcagatccg cgaggtgttc tcgcgccagg tgatgcaggg caagggcccc atggccgttc
     8041 cgcagcgggc gaccgtcgaa gcgtgagccg ttcgcggcgg gccgagttga agcggcgctc
     8101 cagcatccca tgttgaggcg gtgccgatgg cggtccgagt gtccccttca tgggccgccg
     8161 gaagcgcacg caccctagcg gtgcatccct ccctgaactc ggccatcccg gcctgaccgg
     8221 ggtggccgtt ttttattcgg cggcggtcgc cagctgccgg cccatcagcc ggtcgccgag
     8281 gcccagcgcg agcgcttcga gcagcgaggc gaccgagtcg aagcccggcc cgggcgagtc
     8341 gaggcgctca ttgaggtagg accggcggtc gatctcgacc tgcagcgcgt gaatgccgcg
     8401 ctccggagcc ccgtggcgct cgacgatgtg gccgcccgcg aacgggtcgt tgaggcccgc
     8461 ggcgaagccg gcggcccccg cgatccggac cgcctcggcg ctcagccacg gcgcggcgct
     8521 gcgcccgtgg cggtcgccga acacgatcgg cgggaccccg cgcgcgggcg gcggcatcga
     8581 atggcagtcg agcaggagcg cgcagccgaa ccgcccggcg agcttcccga gctgctgctc
     8641 gagcgcccga tggtagggcc ggtgggcctg gtcgagccgg tcctcgagct gcgccgggct
     8701 gatcggccgc cgccacagat agccgtggct ctgggtgcgg ccggggacga tcccgagccc
     8761 gccgcgggcg cgggcgctga ccttgccccg ccccgggccg ccctcgatca ccgacgggtc
     8821 gacttcgtcc tcgccgcggt tgcagtcgat cgcggcgcgc ggcgcgcggg cgatcaccgc
     8881 cgcgatcccg cgctcgaccg cccgccacgc cagccggtcg accagcgggt cttcgagcgc
     8941 ggcgagcgcc cggcgcccgc ccgaggccat cgccaccagc cagtcgggat agtcgcggcc
     9001 cgaatgcggc accgacagga gcaccggcag gccggcgcgc ggcgggtgga cgatgggcgc
     9061 ggggaggggc gcaatcgggg actcggggag gctcactgaa gcgtctcaat ctaggacgat
     9121 cggggcccgg tcaacgcccg ggcggaagga atagcggctg gaaaatatta aaaggtctgc
     9181 gcataagaag gggcaatggc gagaatcctg ctggccgaag acgacacgtc gatgcgcgag
     9241 tatctgcagc gcgcgctgca gcgcgtcggc tacgaggtcg aggcggtcgg ctgcgggacc
     9301 gaggcgatgc cgctcctgga agccgggcgc ttcgacctgc tcctgaccga catcgtcatg
     9361 ccggaaatgg acggcatcga gctcgcccag aaggcgtcgg tgatcgatcc gtcgatccgg
     9421 gtgatgttca tcaccggctt cgccgcggtc gcgctccagg gcgggcggac ggcgcccgag
     9481 gccaagctgc tgtcgaagcc gttccacttg aaggacctcg tcgccgaggt cgaccgcatc
     9541 ttccagaccg aggaccagca cgggcggctc taggccggcc ctaaatcgct gcgtcgccga
     9601 ggttgcccga tcgccgatcg gcccttagat agccggtcga gtgggcgtgt agctcagtgg
     9661 tagagcactg tgttgacatc gcaggggtcg caagttcaat ccttgccacg cccaccattt
     9721 ccccttctcg gcagctccaa cggggtgaac ccgtgccacg gcacggcagt gcgcggaaca
     9781 tcgcggcttt gcgaccgttc gcggttgaga gcgcgaacct ttccaaagga gaaggagcat
     9841 gagcgcgaac ggactcgagg tgttcgacaa gaccctcgaa accacccaca tctggctcaa
     9901 ggagatcatg gccgacatcg gccccgaccg gcaggtcgcg tggaaggtgc tgtcggtggt
     9961 cctccacaag ctccgcgacc ggctgccgct gggcctcgcc gcgcatctcg gcagccagct
    10021 gccgttgctg gtccgcggcg tctattacga ccagttcgag cccggcaagc tgccgacgac
    10081 ctgcaacagc cgcgaggagt ttgtcgccga ggtcgccgaa tggctgaccg acaccaggcc
    10141 ggtcgacccg gacctcgcgg tgcgctcggt gttcaaggtg ctgtcgaacc acgtctcgga
    10201 gggccaggtc gccaaggtcc gcgaagcgct gccgcgaggc ttgcggcaga gctggatcga
    10261 cgcggaggcc agggaacccg ccgacagcga cgcctgaggg gaggcgaacc agcgccgagg
    10321 ccggggtcct agcgcttcgg cttgacgaac ttcagcgtca tccggtcgct ctcgccgatc
    10381 gccagatatt tctcgcggtc cttgtccttg agagcgagcg agggcggaag cgtccacacc
    10441 ccgtccggcc agtcggccgt atccttcggg ttggcgttga tctccgaggc ggcggcgagg
    10501 cggaaacccg cctgttcggc cagccggcga acggtcgaaa ccttgatgta gccgctgctg
    10561 cgctcgcgct cgtcgctggc gtcctccggc agccggtggt cgacgatgcc cagagtgccg
    10621 ccgggcttga gcatcgcgtg aatctggcgg aacgcctcgg cgctgtagtc ctgcttgtcg
    10681 tcgcgccggt agcccatccg ccaattgtgg acgttgcgga aggtgagaac gacgtcggcg
    10741 ctgcccgccg gcaccggcgt gtcctcggca tcgaacaccg ggaagtcggc ggtcagcacc
    10801 gggccgtaga gggccgggtt tgcggccttg agcttggcga tgccggtcat tccccactcg
    10861 ggagcggcgg catagagccg gccgccgccg gccgcgaggt agggcgccag gatctcggtg
    10921 taccagccgc cgcccggcca cagctcgacg accgtgtcgc tcggcctcac tccgaagaag
    10981 gccagggtct cggccggatg gcgatagcga tcgcgggcga ggttggcggg cgtccgggac
    11041 ggggaggcaa ctgcaaccgc gagggcctcc ggcgatacct gggcagaggc gggcccgacg
    11101 ggggcacagg cgggcaaggc gagggccgcc agcagcagac cgaacagtgg acgagacagc
    11161 atgaccgctc cttcaaaatg aggcagcggt cgccgtggca agtctggcgg ttcgatgcaa
    11221 ccgtcgggcg gtcgaaggat tagaggcagg cctccaggta cggctggtcg aagccgaact
    11281 gcttggcttt ctcgagcgtg tagggcctca ggcccatcga gcggtactcg ccgaggatct
    11341 tcccgtcggg gctctcgtcg agatactcga acttgaacag ctcctgggtg acgatcacgt
    11401 cgccttccat gccgatcacc tcggtgatgt tggtcgtccg gcgcgagccg tcgcgcaggc
    11461 gcttgacctg gacgatgagg tcgaccgaat cggcgatctg gcgggaaatc gcctccttcg
    11521 ggatcttgat gtccgacatc atcaccatgt tctccatacg cgcgaggcac tcgcgcgggg
    11581 agttggagtg gagcgtcgcc atcgagccgt cgtggccggt gttcatcgcg gccagcagat
    11641 cgaagcattc ggggccgcgg atttcgccca ggatgatccg gtcggggcgc atgcgcaacg
    11701 cgttgatgac caggtcgcgg atggtgatcg cgccctggcc ctcgaggttc gccgggcggg
    11761 tttcgagcgg cagccagtgc ggctgctgaa gccgaagctc ggccgcgtcc tcgatggtga
    11821 tcacgcgctc gccggggtcg atcatcttgc tgagcgcgtt gagcatcgtc gtcttacccg
    11881 aaccggtgcc gcccgagatg atgacgttca ttcggctggc gccggcgatt ttgagcaagg
    11941 tcgccatctt gtcggacatc gatccgaacc cggtcatcat gtccagtgtg atcggctttt
    12001 cggagaattt acggatcgag atggcggtgc cgcgaagcga cagcggcggg ataatgacgt
    12061 tgacgcggct gccgtcctgg agccgggcgt cggcgagcgg agtcgtctga tcgacccggc
    12121 ggccgacctt gttgacgatc cgctgggcga tctggaacag atgctcctcg tcgcggaact
    12181 ggatctgggc gagctcgagc ttgcccttgc gctcgatgaa gcactggtcg gggccgttga
    12241 ccatgatgtc gctgatcgcc gggtcggcga gaagctcctc gagcggcccg aggccgagaa
    12301 gctcgtcgac cagcaccttt tcgagcgcga actgctcgcg ccggttgagg gtgatcttca
    12361 gctcggcgag cacctcgccg atgatcggcc ggaactcctc ggccagctcg tccttgctca
    12421 gggtcgccgc ggcttccgga tcgacccgtt cgagcagccg cgggagcacc tgctccttga
    12481 tccggtggac cgacgcctcg aagccttcgg ccttgctgct gccctgctcg ccgctctggt
    12541 tctggcgcgc ggtcaggcgg tccatcgccc ccatcgagcc gccgtcgccg gcgccgccga
    12601 tgtcgtcggg aaggctttcg acctgcggag cgatctcctc cagcggcggg aactggtcac
    12661 cgccggacgg gacggcctgg gccttgccgc ccggtgattt cattggacgg gccacgccga
    12721 agctggggcg ctgcccgcct ccgctcatgc cgccacgctt gccgaatgcg ctcattccaa
    12781 ccccatcgcc atgcggcgtc ccgcacaaag tcgagatggt gaataggggg caaactttta
    12841 gaaaagccta accgatcatc ctccccggga cggggaggtg gcagcgcgga gcgctgacgg
    12901 aggggaccca gcgacccgca ccacgccccc tccaccatcg aatcttcgtt cgctggtccc
    12961 cctccccgtt ccggggagga ttagcccgtg ctttccgcga gcaccgtgag gccgcgctcg
    13021 ctgacttcgg cgaagccgcc gctgatcgcg atcttctccg gctgagcgcc ggcggtgcgg
    13081 tagatggcca gctcgccgtc gcgaagcgtg ctcatgtagg gcgcgtggcc ggccagcacg
    13141 ccgaactccc cttcggtgcc cggcacgacg accatgtgga cgtcctcgga catgacgagc
    13201 ttctcgggcg tgacgagctc gaagtggagg tcggccaccg cttacgcgtc ctcggccagg
    13261 cgctggccct tggcgaccgc ttcctcgatc ccgccgacca tgtagaaggc cgcctcgggc
    13321 agatggtcat actcgccgtc gaccaccgcc ttgaacgagc ggatggtgtc ctcgagctgg
    13381 acgaacttgc cggggatgcc ggtgaacacc tcggcgacgt ggaacggctg cgacaggaag
    13441 cgctggatct tgcgggcacg gctgacgacc agtttgtcct ctacgctgag ctcgtccatg
    13501 ccgaggatgg cgatgatgtc ctgcaggctc ttgtagcgct gcagggtctc ctggacggcg
    13561 cgggcggtgt cgtaatgctc ctggccgacg gtgcggggtt cgagcactcg gctggtcgaa
    13621 tcgagcgggt cgacggccgg gtagatgccg agctcggaga tcgcgcgcga cagcaccgtg
    13681 gtcgcgtcca agtgggcgaa cgaggtcgct ggcgccgggt cggtcagatc gtcggccggc
    13741 acgtagatcg cctggaccga ggtgatcgag cccttgttgg tcgaggtgat ccgctcctga
    13801 agcgagccca tgtcggtggc cagagtcggc tggtagccga ccgcggacgg gatgcggccg
    13861 agcagcgcgg agacttccga gcccgcctgg gtgaagcgga agatgttgtc gatgaagaac
    13921 aagacgtcct gcccttcggc atcgcggaaa tattcggcga tcgcgaggcc cgacagagcg
    13981 acccgggcgc gcgctcccgg cggctcgttc atctggccgt agaccagggc gaccttcgag
    14041 ccctcggaga tcgcgttgcc gtccttgtcc ttggcgatca cgcccgcgtc gaggaactcg
    14101 tggtagaggt cgttgccctc gcgggtgcgc tcgccgacgc cggcgaacac gctggttccg
    14161 ccgtggccct tggcgatgtt gttgatcagc tcctggatga gcaccgtctt gccgacgccg
    14221 gcgccgccga acaggccgat cttgccgccg cgcgcgtagg gcgcgagcag gtcgatgacc
    14281 ttgatgccgg tgaccaagat ggccgcttcg gtcgactggt cgacgaactt cggcgcttcg
    14341 gcgtggatcg aggcgaactt gtcgctgccg atcgggccac gctcgtcgat cggctcgccg
    14401 acgacgttca tgatccggcc aagcgtcttg gggccgaccg gaacctggat ttgcgaaccg
    14461 gtggcggtga cctgctggcc gcgggtcagg ccgtcggtcg agtccatggc gatggtgcgg
    14521 acgatgttct cgccaaggtg ctgagcgact tcgagcacga gccgctggcc atggttgtcg
    14581 gtttccagcg cggccaggat cggcggcaac tcgccttcga aagcgacgtc gacgaccgcg
    14641 ccgatcacct gcgcgacctt gccgacaatg tttccgccag tggtgggcga cttgggagct
    14701 ggcgcgctct tggtcgcggc cttcttggtg cgggtcttgg gctcagcggc ggttgccatg
    14761 ttcggtttcc ttgcctt
//
LOCUS       DASTZO010000283         4866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_441374,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000283 DASTZO010000000
VERSION     DASTZO010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_441374"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..1287)
                     /locus_tag="VFU20_07980"
     CDS             complement(82..1287)
                     /locus_tag="VFU20_07980"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU5286429.1"
                     /translation="MAGLNTLDDLPPDLAGKRVLVRVDLNVPMQDGAISDDTRLRAAV
                     PTIAELSDRGAIVLLLSHFGRPKGRSRPDMSTALLVRPLAELTGRPVRFIEDCQGPEA
                     SRAITTMTEGSIAILENTRFHAGEEKNDPELATAMAALGDFYVNDAFSSAHRAHASTE
                     GIAHRLPSFAGRAMQAELDALEKALGEPERPVAALVGGAKVSTKLGVLGHLVGKVEHL
                     IIGGGMANTFLAARGVDVGKSLAEHELTGEATAIFARAEAAGCTIHLPYDVVVATEFA
                     PNPPGVRTANVHEITPDEMILDVGPDAVEAFGDVLKTCRTLVWNGPLGAFETPPFDAG
                     TVALAKTAAALTKEGSLVSVAGGGDTVAALNHAGVTEDFTFVSTAGGAFLEWMEGRTL
                     PGVAALQKQ"
     gene            complement(1290..2297)
                     /gene="gap"
                     /locus_tag="VFU20_07985"
     CDS             complement(1290..2297)
                     /gene="gap"
                     /locus_tag="VFU20_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU5286430.1"
                     /translation="MTRVAINGFGRIGRLVARAILERPDCRLELVAINDLADAKSNAW
                     LFARDSVHGRFPGEVAADGNDLVIDGTRIRVTAERDPAKLPHRDNGVELALECTGFFT
                     DREGGQKHLDAGAKRVLISAPAKGADLTVVYGVNHDKLTAEHTIVSNASCTTNCLAPV
                     AKVMNDVFGIERGLMTTIHSYTNDQKILDQIHGDLRRARAAAMSMIPTTTGAARAVGE
                     VLPELKGKLDGSAVRVPTPNVSLIDLTFVPGRETSKEEVNAALKAAADSGPLKGVLDY
                     SDEPLVSIDLNHNPASSTVDSLETAVIEGKLVRVVSWYDNEWGFSNRMVDTAAAMATL
                     G"
     gene            complement(2294..4282)
                     /gene="tkt"
                     /locus_tag="VFU20_07990"
     CDS             complement(2294..4282)
                     /gene="tkt"
                     /locus_tag="VFU20_07990"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU5286431.1"
                     /translation="MQPTQRRLANAIRALAMDAVEAANSGHPGMPMGMADAATALFTR
                     HLKYDPADPHWPDRDRFVLSAGHGSMLIYALLYLTGYARPTLDDIKRFRQLHSPCAGH
                     PENFELAGVEATTGPLGQGLAMAVGMAIAERHLNAVYGDELVDHRTFVLASDGDLMEG
                     VNHEAVGLAGHLKLGRLIVLWDDNRITIDGSTDLSRNEDVVARYAAGGWHTAECDGLD
                     AGKVERALDEAIADERPSLIRCRTIIGYGAPNKQGTAATHGAALGRQEVEAARKELGL
                     ETKEFTVPDDVLAAWREVGRRGAARRAEWNRRLEASKKKDEFSARIAGKLDDQWLKAH
                     LNALLADPPTLATRKASEMALEAINAAVPATIGGSADLTGSNNTKTKTQEPLTADNYA
                     GRYIYYGIREFGMAAAMNGMALHGGIVPYGGTFLIFSDYCRPAIRLSALQKAKVIYVM
                     THDSIGLGEDGPTHQPIEHLQSLRAMPQLEVFRPADAVETAECWAAALAGGGPSVLAL
                     TRQNLSPVRTKAARENLCARGAYRLKKAGGPRKVILIATGSEVEIALGVAERLEAQGI
                     GADVVSMPSTERFDAQDAAYREDILPDVSNREILRVSIEAGATFGWERYTGLHGLRIG
                     IDRFGGSAPAADLYDHFGLTPDAIAPRVVELLQKRDML"
     gene            4423..4599
                     /locus_tag="VFU20_07995"
     CDS             4423..4599
                     /locus_tag="VFU20_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286432.1"
                     /translation="MDDGSLSQALERADRALGRIERALAARSHQVQGREQELRSKVRE
                     AVAELDQLIRSTGE"
     gene            4601..>4866
                     /locus_tag="VFU20_08000"
     CDS             4601..>4866
                     /locus_tag="VFU20_08000"
                     /inference="COORDINATES: protein motif:HMM:NF017016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEU5286433.1"
                     /translation="MAEVDLTIAGRPYRVACRAGEEDSLRAAAALVDAKSREALAGLG
                     TMSEARQLLFASLLLADQLIDQRGPDAVPASIDPKLAESAEALAG"
BASE COUNT          677 a         1649 c         1736 g          804 t
ORIGIN      
        1 agccgccgct ctggtcgagc gcggcgatga agccgctgcc gtcgcggatc ttggtcatca
       61 tctgctggtc catggtcatc cctactgctt ttgaagcgcc gccacgccgg gaagcgtgcg
      121 gccttccatc cattcgagaa acgctccgcc ggccgtcgac acgaacgtga aatcctcggt
      181 gacgcccgca tggttgagcg ccgcgaccgt gtcgccgccg cccgcgacgg agacgaggct
      241 cccttccttg gtgagggcgg ccgcggtctt ggcgagcgcc accgtcccgg cgtcgaacgg
      301 cggcgtctcg aacgccccaa gcggcccgtt ccagaccagc gtcctgcagg tcttgaggac
      361 atcgccgaac gcctcgaccg cgtccgggcc gacgtcgagg atcatctcgt cgggcgtgat
      421 ttcgtggaca ttggcggtgc gcacgccggg cgggttgggc gcgaactcgg tcgcgacgac
      481 gacgtcgtag ggcagatgga tggtgcagcc ggcggcctcg gcccgggcga agatggcggt
      541 cgcctcgccg gtcagctcgt gctcggcgag cgacttgccg acatcgaccc cgcgcgcggc
      601 gaggaaggtg ttggccattc cgccgccgat gatcagatgc tcgaccttgc cgaccaggtg
      661 gccgagcacg cccagcttgg tcgagacctt ggccccgccg accagggccg cgaccggccg
      721 ctcgggttcg ccgagcgctt tctcgagcgc gtcgagctcg gcctgcatcg cgcgccccgc
      781 gaagctcggg agtcgatgcg cgatgccctc agtggaggcg tgggcgcggt gcgcggacga
      841 gaaggcgtcg ttgacgtaga agtcgccgag cgccgccatc gccgtggcca gttcgggatc
      901 gttcttttcc tcgccggcgt ggaaacgggt gttctcgagg atcgcgatcg agccctcggt
      961 catggtggtg atcgctcggc tcgcctcggg gccctggcaa tcctcgatga agcgcaccgg
     1021 gcggccggtg agctcggcga gcgggcggac cagcagcgcg gtcgacatgt cgggccggct
     1081 ccggcccttg ggacggccga aatgcgacag caacaggacg atcgctccgc ggtcgctcaa
     1141 ttcggcgatg gtcggcaccg cggcgcgcag ccgggtgtcg tcgctgatcg ccccgtcctg
     1201 catcggcacg ttcaaatcga cgcgcaccag cacccgcttg ccggcgaggt ccggcggcaa
     1261 atcgtcgagg gtgttgagcc cggccaccct cagccgagcg tggccatcgc ggccgcggtg
     1321 tcgaccatcc ggttcgagaa gccccattcg ttgtcgtacc aggaaacgac ccgcaccagc
     1381 ttgccctcga tcaccgcggt ctcgaggctg tcgacggtcg agctggccgg attgtggttg
     1441 aggtcgatcg agaccaacgg ctcgtcgcta tagtcgagga cgcccttcag gggcccgctg
     1501 tcggccgcgg ccttcaaagc cgcgttgacc tcttccttcg aggtctcgcg cccgggcacg
     1561 aaggtcaggt cgatcaggct gacgttgggc gtcggcaccc gcacggccga gccgtcgagc
     1621 ttgcccttga gctccggaag aacctcgccg accgcccggg cggcgccggt ggtggtcggg
     1681 atcatcgaca tcgccgcggc gcgggcgcgg cgcaaatcgc cgtggatctg gtcgaggatc
     1741 ttctggtcgt tggtgtagct gtggatggtg gtcatcagcc cacgctcgat gccgaaaacg
     1801 tcgttcatca ccttggcgac cggcgccagg cagttggtcg tgcacgacgc gttggagacg
     1861 atcgtgtgct cggcggtgag cttgtcgtga ttgactccgt agacgaccgt caggtcggcg
     1921 cccttggcgg gagccgagat cagcacccgc ttggctccgg cgtcgaggtg cttctgcccg
     1981 ccttcgcggt cggtgaagaa gccggtgcat tccaacgcca gctccacgcc attgtcgcgg
     2041 tgcgggagct tggccggatc gcgctcggcg gtgaccctga tgcgcgtgcc gtcgatgacg
     2101 aggtcgttgc cgtcggccgc gacctcgccg gggaagcggc cgtggacgct gtcgcgggcg
     2161 aacagccagg cgttcgactt ggcgtcggcg aggtcgttga tcgcgaccag ctcgagcctg
     2221 cagtccggcc gctcgaggat cgcccgggcg accagccggc cgatgcgccc gaagccgttg
     2281 attgccactc tcgtcatagc atgtcccttt tctgcagcag ctcgaccacc cgcggagcga
     2341 tcgcgtcggg ggtcaagccg aaatggtcgt agaggtcggc ggccggcgcc gaaccgccga
     2401 agcggtcgat gccgatccgg aggccgtgca ggccggtgta gcgctcccag ccgaaggtcg
     2461 cgccggcctc gatcgacacg cgcaggattt cgcggttgga gacgtcgggc aagatgtcct
     2521 cgcggtaggc ggcgtcctgg gcgtcgaacc gctcggtgct cggcatcgac acgacgtcgg
     2581 cgccgatgcc ctgcgcttcg agccgctcgg cgaccccgag cgcgatctcg acctccgagc
     2641 cggtggcgat caggatcacc ttgcgcggcc cgcccgcttt cttcagccgg taggcgccgc
     2701 gcgcgcacaa attctcgcgc gccgccttgg tgcgcaccgg gctcagattc tggcgggtca
     2761 gcgccagcac gcttggcccg ccgccggcca gcgccgcggc ccagcattcg gcggtctcga
     2821 ccgcgtcggc cggccggaac acctcgagct gcggcatcgc ccgaaggctt tgcagatgct
     2881 cgatcggctg atgggtcggg ccgtcctcgc caaggccgat cgaatcgtgg gtcatcacat
     2941 agatgacctt cgccttctgc agcgccgaca ggcggatcgc cgggcggcaa taatcggaga
     3001 aaatgaggaa agtgccgccg taggggacaa tgccgccgtg caacgccatg ccgttcatcg
     3061 ccgcggccat gccgaactcg cggatcccgt aatagatgta gcgcccggcg taattgtcgg
     3121 cggtcagcgg ctcctgggtt ttggtcttgg tgttgttcga gccggtcagg tcggccgagc
     3181 cgccgatggt ggcggggacc gcggcgttga tcgcctcaag ggccatttcc gaggccttgc
     3241 gcgtggcgag cgtcggcgga tcggcaagca gagcgttgag atgggccttg agccattggt
     3301 cgtcgagctt gcccgcgatg cgcgcggaga actcgtcctt cttcttgctc gcctcgagcc
     3361 ggcggttcca ctcggcccgt cgcgccgcgc cgcgccggcc gacctcgcgc caggcggcga
     3421 ggacgtcgtc ggggacggtg aactccttgg tctcgagccc caattccttg cgcgccgcct
     3481 cgacttcctg cctgccgagc gcggcgccgt gggtggcggc ggtgccctgc ttgttgggcg
     3541 ccccgtagcc gatgatcgtc cggcagcgga tcaggctcgg ccgctcgtcg gcgatcgcct
     3601 cgtccagtgc gcgctcgacc ttgccggcgt cgagcccgtc gcactcggcg gtgtgccagc
     3661 cgccggccgc gtagcgcgcg acaacgtcct cgttgcgcga caggtcggtc gagccgtcga
     3721 tggtgatcct attgtcgtcc cacagcacga tcagccggcc gagcttcaga tgcccggcga
     3781 ggccgaccgc ctcatggttg acgccctcca tcaaatcgcc gtcgctggcg aggacgaagg
     3841 ttcggtggtc gaccagctcg tcgccgtaga ccgcgttcag gtgccgctcg gcgatcgcca
     3901 tcccgaccgc catcgccagc ccctgcccca aaggcccggt ggtcgcctcg accccggcca
     3961 gctcgaaatt ctccgggtgg ccggcgcacg ggctgtgcag ctggcggaag cgcttgatgt
     4021 cgtcgagcgt cggccgggcg tagccggtca ggtagagcag agcgtagatc agcatcgagc
     4081 cgtggccggc cgacaggacg aagcggtcgc ggtcgggcca gtgcgggtcg gcggggtcgt
     4141 acttgaggtg ccgggtgaac agggcggtgg ccgcgtccgc catccccatc ggcattcccg
     4201 ggtggccgct gttggcggcc tcgaccgcat ccatcgccaa ggcgcgaatg gcgttggcaa
     4261 gtcggcgttg ggtcggctgc aatcctcaag gctccctgaa ggcatttggc gacgggatca
     4321 gccgcgcgcc ggttcgggcg ggccgatgcg cgccctttgg gcggtgagcc gttaggcgtc
     4381 aatgcttgcc cggaccgtcc atcttgcgta gcgagaaatg caatggacga cggctccctt
     4441 tcgcaagctt tggagcgcgc cgaccgagcg ctcggccgga tcgagcgggc gcttgccgcc
     4501 cgcagccacc aggtccaggg gcgcgagcag gagctccgtt ccaaggtgcg cgaggcggtc
     4561 gccgagctcg accagctgat tcgctcgacg ggggagtgac atggccgaag tcgacctgac
     4621 catcgccggc cgcccgtacc gggtcgcgtg ccgggcgggc gaggaggaca gtctgcgcgc
     4681 cgccgcggcg ctggtcgacg ccaagagccg cgaggcgctg gccgggctgg gcacgatgtc
     4741 cgaagcgcgc caattgctgt tcgcctcgct gctgctcgcc gaccagctga tcgaccagcg
     4801 cgggcccgac gcggtgcccg cgagcatcga ccccaagctt gccgaaagcg ccgaggcgct
     4861 tgccgg
//
LOCUS       DASTZO010000284         2972 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_494121,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000284 DASTZO010000000
VERSION     DASTZO010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2972
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494121"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..454
                     /locus_tag="VFU20_08005"
     CDS             134..454
                     /locus_tag="VFU20_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286434.1"
                     /translation="MGRRVKEFIEIEDHSSLDDLIARLSEVRASLPPDSEAELRLRGD
                     DVFGRKLSISFFRDQTAEEADCEKRYAEACKESKERDLARLQQELGVVCYAPNRKGKL
                     RIVA"
     gene            complement(479..2233)
                     /locus_tag="VFU20_08010"
     CDS             complement(479..2233)
                     /locus_tag="VFU20_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU5286435.1"
                     /translation="MLIVLLAYLGGVLTIVSPCILPVLPFVFARAERGFARGALPLLA
                     GMALTFAAVATLAAVGGGWAVRANEAGRWAALALLALFGLALVVPSLSERMMRPLVAL
                     GSRLSERASARAASRPGGDGIGSSLLLGAATGLLWAPCAGPILGIIFTAAAIEGATLN
                     TTLLLLAYALGAATSLALALLVGGRLFARMKRSLGAGERIRQVLGVLVLVGVSAIALG
                     LDTRVLAKLSATQTASLESSLARKLGVGGIAADSKVATGAGGQLILQVEGRLPPLDGL
                     GPWLNSPPLSREQLKGKVVLIDFWTYSCINCLRSIPYVRAWHERYARDGLVVIGVHAP
                     EFAFEREPANVRKAVQDLGIRYPVALDNKYRLWTALKNNYWPAHYFIDARGRVRFHHF
                     GEGKYEQSERVIRQLLAEAGRAPKGGPMARAVKLDDAEAQAAFAALKSPETYVGYKRA
                     ERFVSPGGLVRDRAARYRPAPLRLNDWSLDGEWTVGRQSARAGAAGAKIHYRFHARDL
                     HLVLGSPGGRPVRFRVALDGQAPGADSGGDAAADGSGRVEGERLYQLIRQKGPVRERG
                     FTIEFLDPGVEAFAFTFG"
     gene            complement(2250..2729)
                     /gene="msrB"
                     /locus_tag="VFU20_08015"
     CDS             complement(2250..2729)
                     /gene="msrB"
                     /locus_tag="VFU20_08015"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEU5286436.1"
                     /translation="MIDRRTLLAGGGAAVAAALLLGRSTDAAAARFAVTRTPAEWRRQ
                     LDPQRYRILREAGTERPFSSPLDKERRRGLYRCAGCANPLFASSTKFDSGTGWPSFFR
                     PLQKAIVTREDKSLLMTRTEVLCARCGGHLGHVFRDGPRPTGLRYCINGLALRFQPA"
     gene            complement(2768..2858)
                     /locus_tag="VFU20_08020"
     tRNA            complement(2768..2858)
                     /locus_tag="VFU20_08020"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2822..2824),aa:Ser,seq:gga)
BASE COUNT          478 a         1063 c         1031 g          400 t
ORIGIN      
        1 gctcatttct gcacctcgga tttcaaaacg attcacaggc cggcaacttc cctggggata
       61 aacctggatt agtcgttgcg ctcggattcg agcgagcagt atctagcagc cccggggccg
      121 gggagggtct ggaatgggta gacgagtcaa agagttcatc gagatcgagg accatagctc
      181 gctcgatgat ttgatcgcca ggcttagcga agtgcgcgcc tcccttccgc cggactccga
      241 agccgagttg cggctccgcg gggacgacgt gttcggccgc aagctgtcga tctctttctt
      301 ccgcgaccag acggccgagg aggccgactg cgaaaagcgc tacgccgaag cgtgcaagga
      361 atccaaggag cgcgacctcg cccggctcca gcaggagctc ggcgtggtct gctacgcgcc
      421 caatcgcaag ggcaagctgc gcatcgtcgc ctgactgtgg cgcggcggtt agccgcgcct
      481 agccgaaggt gaaggcgaac gcctcgaccc cggggtcaag gaactcgatc gtgaagccgc
      541 gctcgcgcac cggccccttc tgccggatca gctggtacag ccgctcccct tcgacccgcc
      601 cactgccatc cgccgcggcg tcgccaccgg aatccgctcc cggggcctgg ccgtccaggg
      661 ccacccggaa ccgcaccggc cggccgccgg gcgagccgag caccaggtgc aggtcgcgcg
      721 cgtggaagcg atagtggatc ttcgctccgg cggcgcccgc gcgggcgctc tgcctgccga
      781 ccgtccactc gccgtcgagc gaccagtcgt tgagcctgag cggcgccggc cggtagcgcg
      841 ccgcgcggtc gcggaccagt ccgcccggcg acacgaaccg ctccgcccgc ttgtaaccga
      901 cataggtctc cggcgacttc agcgccgcga acgccgcctg ggcttcggcg tcgtcgagct
      961 tgaccgcccg cgccatcggg ccgcctttcg gcgctcgtcc ggcctcggcg agcaactggc
     1021 ggatgacccg ctcggactgc tcgtacttgc cctcgccgaa atgatggaag cgcacccgcc
     1081 cccgggcgtc gatgaaataa tgcgcgggcc agtaattgtt cttgagcgcg gtccacagcc
     1141 ggtacttgtt gtcgagcgcg accgggtagc ggatgccgag gtcctggacc gccttgcgga
     1201 cattggccgg ctcacgctcg aacgcgaact cgggcgcgtg gaccccgatc accaccagcc
     1261 cgtccctggc gtagcgctcg tgccacgcgc gcacgtaggg gatcgagcgc aggcaattga
     1321 tgcagctgta ggtccagaaa tcgatcagca cgaccttgcc cttgagctgc tcgcggctga
     1381 gcggcgggct gttgagccac gggccgagcc cgtcgagcgg cggcagccgg ccttcgacct
     1441 gcaggatcag ctgcccgccg gcgccggtcg cgaccttgct gtcggcggcg atcccgccga
     1501 ctccgagctt gcgcgccagg ctggattcga ggctcgcggt ctgggtcgcg gacagcttcg
     1561 ccagcacgcg ggtgtcgagc ccgagggcga tcgccgacac tccgaccagc accagcacgc
     1621 cgagcacctg ccggatgcgc tcgccggcgc cgagcgaccg cttcatccgg gcgaacagcc
     1681 tgccgccgac cagcagcgcc agcgccagcg acgtcgccgc cccgagggcg taggcgagca
     1741 gcagcagcgt cgtgttgagg gtcgcgccct cgatcgcggc ggcggtgaag atgatgccga
     1801 ggatcggccc cgcgcacggc gcccaaagca ggccggtcgc ggcgccgagc agcagcgacg
     1861 aaccgatgcc gtcgccgccc ggccggctcg cggcgcgcgc cgacgcccgt tccgaaaggc
     1921 gcgagcccag cgccaccagc ggccgcatca tccgctccga cagcgacggc acgaccagcg
     1981 ccagtccgaa cagggccagc agcgccagcg ccgcccagcg gccggcctcg ttggcgcgga
     2041 ccgcccagcc gccgccgacc gcggccaggg tcgcgaccgc ggcgaaggtc agcgccatcc
     2101 cggcgagcag cggcaaagcg ccgcgcgcga agccgcgttc ggcgcgcgcg aacacgaacg
     2161 gcagaaccgg caggatgcac gggctgacga tggtcaggac gccgccgaga taagcgagca
     2221 ggacgatcag cacggcgggc tcggcgggct cacgccggct ggaaccggag cgcgagcccg
     2281 ttgatgcaat agcgcaggcc ggtcggccgc ggcccgtcac ggaagacatg gccgagatgg
     2341 cccccgcagc gagcgcacag cacctcggtg cgggtcatca acaggctctt gtcctcgcgg
     2401 gtgacgatgg ctttttgcag gggccggaag aagctcggcc agccggtgcc gctgtcgaac
     2461 ttggtgctcg aggcgaacag cggattggcg catcccgcac agcgatagag gcctcggcgc
     2521 cgctccttgt cgagggggct cgagaagggc cgctcggtcc ccgcctcgcg caggattcga
     2581 tagcgctgcg ggtccagctg ccggcgccac tcggcgggag tcctggtgac agcgaaccgc
     2641 gccgccgccg catcggtcga gcgcccgagc agcagcgccg ccgccaccgc cgcgccgccg
     2701 cctgcgagca aggtcctgcg gtcgatcatg caaaggcccc ttagcgcgaa tgcacctgaa
     2761 cggcagatgg cggagcggga gggattcgaa ccctcgatac ggttttgccg tatactcact
     2821 ttccaggcga gcgccttcga ccactcggcc accgctccgc atgcactgga agagggcgtc
     2881 atctagggtg aggcgcgttt gcgggcaagc ttgccgctgc tgcggcgcga ccctagggcg
     2941 ctgccatgga cgaggccgag catcgccgcc gc
//
LOCUS       DASTZO010000285         3299 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_650206,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000285 DASTZO010000000
VERSION     DASTZO010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3299)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3299)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3299
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650206"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1581
                     /locus_tag="VFU20_08025"
     CDS             <1..1581
                     /locus_tag="VFU20_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5286437.1"
                     /translation="GIELLPSGRASLTIGPGLTIQGPSGAKQVKLAFLRGILSSPVPV
                     WLDEQNKYFADVGWISLMPAGFEANAKAMRDRQEDATAAEVKSIARRFLTAEARAPVL
                     FDNVRLFDADRGVFVDNRAVLAADGKIKAIVAAGSMTPPPNVRVIDGRGKTLVPGIWD
                     SHMHIGDDWDVLANMANGMTSFRSPGTMIDRAQSVTRRRASGDLLVGEPFVSMIIDRK
                     DPLAAQGALTVTSEAETIAAVRKIKDGGLWGVKFYTSMNPAWIAPGAAEAKRLGLHVH
                     GHVPATMRPLEAVRAGYDELTHLNFVVMQAMPKEVVDKANTAERIEGPARFTKDVDWS
                     SPEMRAFFAELKQRGTIVDPTLVIFEQALTMDGGKTAPAYVPYEGIISPVLDRSFKSG
                     GHPLVKGYTRDDYRKSFAKMVELVRVLHEARIPIVAGTDGSGIELVRELELYRQSGFT
                     AAEALQSATIVPARVVGADKRTGSIAVGKEADMVLVDGDVAKDLEALRRVVTVVSDGY
                     VMDGDALRKAAGYTGRPK"
     gene            complement(1585..2007)
                     /gene="mscL"
                     /locus_tag="VFU20_08030"
     CDS             complement(1585..2007)
                     /gene="mscL"
                     /locus_tag="VFU20_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEU5286438.1"
                     /translation="MLMEFRDFVARGNVLDLAVAVIIGAAFATITASLTDDIIMPVVG
                     AIFGGLDFANYFTILGPVPDNYAGSLASYADLKKAGVAVLGWGRFVTVLINFVILAFV
                     IFLLVRWANRLTPRGADAPAGPSEVELLAEIRDELRKR"
     gene            2120..2716
                     /locus_tag="VFU20_08035"
     CDS             2120..2716
                     /locus_tag="VFU20_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU5286439.1"
                     /translation="MTALRRFGLLAPLAAFSLAGCGLNTVPTAEEQVNAAWGDVQSEY
                     QRRADLIPNLVRTVERYADQERDVLTEVTNARARASSIQLRAEDLDDPAKVRAFNDAQ
                     TQLGGSLGRLLATFEAYPDLKSNQNFLTLQSQLEGTENSILVSRRDYNEAVRQYNTTI
                     RTFPDAIGAKIFYGAKPKVPFEAATGAQTAPTVDFNAS"
     gene            2718..>3299
                     /locus_tag="VFU20_08040"
     CDS             2718..>3299
                     /locus_tag="VFU20_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU5286440.1"
                     /translation="MTRLFAVLWLALIALAAPAAAQTFPQLTGRVVDQADLLRPEQEL
                     DLSSKSAALEAQTGRQFVVATVNSLEGRTIEDYGYRLGRHWKIGDEKRDDGVLLLVAP
                     NEKKVRIETGYGARVFLTDAVSSVIIRESILPRFKAGDMGGGITAGADQIVKMMSLSP
                     QEAQRQAGAAERREAQRAESGPGLIPVVFWVMIA"
BASE COUNT          562 a         1119 c         1141 g          477 t
ORIGIN      
        1 ggcatcgagc tgcttccgag cgggcgggca tcgctgacca tcgggcccgg cctgaccatc
       61 caggggccgt ccggggccaa gcaggtgaag ctcgccttcc tgcgcgggat cctgtcgtcg
      121 ccggtgccgg tatggctcga cgagcagaac aaatatttcg ccgacgtcgg ctggatctcg
      181 ctcatgcccg cgggcttcga ggccaacgcc aaggcgatgc gcgaccgcca ggaggacgcg
      241 accgccgccg aggtgaaatc gatcgcgcgg cgcttcctca ccgccgaggc gagggcgccg
      301 gtgctgttcg acaatgtgcg gctgttcgac gccgaccggg gcgtattcgt cgacaaccgg
      361 gcggtcctcg cggccgacgg caagatcaag gcgatcgttg ccgccgggtc gatgactccg
      421 ccgcccaacg tccgggtgat cgacgggcgc gggaagacgc tcgtcccggg catctgggac
      481 agccacatgc acatcggcga cgactgggac gtgctcgcca acatggcgaa cgggatgacc
      541 agcttccgaa gcccgggcac gatgatcgac cgcgcccagt ccgtgacccg gcggcgcgcc
      601 tcgggcgacc tgctggtcgg cgagccgttc gtgtcgatga tcatcgaccg caaggacccg
      661 ctcgcggcgc agggcgcgct gaccgtgacc agtgaggcgg aaaccatcgc cgcggtgcgc
      721 aagatcaagg acggcgggct gtggggcgtg aaattctaca cctcgatgaa cccggcgtgg
      781 atcgctccgg gcgcggccga ggcgaagcgc ctcggcctcc acgtccacgg ccacgtcccg
      841 gcgacgatgc ggccgctgga ggcggtccgc gccggctatg acgagctcac ccacctcaat
      901 ttcgtcgtca tgcaggcgat gccgaaggag gtcgtcgaca aggccaacac cgccgagcgg
      961 atcgaggggc cggcgcgctt caccaaggac gtcgactgga gctcgccgga gatgcgcgcc
     1021 tttttcgccg agctgaagca gcgcgggacg atcgtcgacc cgaccctggt gatcttcgag
     1081 caggcgctga ccatggacgg cggcaagacc gcgccggcct atgtccccta cgaagggatc
     1141 atctcgccgg tgctcgaccg gagcttcaag tccggcgggc acccgctggt caagggctac
     1201 acgcgcgacg actaccgcaa gagcttcgcc aagatggtcg agctggtgcg cgtgctccac
     1261 gaggcccgaa tccccatcgt cgccggcacc gacggctcgg ggatcgagct ggtccgggag
     1321 ctcgagctct accggcagtc gggcttcacc gcggccgagg cgctccagag cgcgaccatc
     1381 gtcccggcgc gagtcgtcgg cgccgacaag cgcaccggct cgatcgccgt cggcaaggag
     1441 gccgacatgg tgctggtcga cggcgacgtg gcgaaggacc tggaagccct gcgccgggtc
     1501 gtcaccgtgg tcagcgacgg ctatgtgatg gacggcgatg cgcttcggaa ggccgcgggc
     1561 tacaccggcc ggccgaagtg atacctaccg cttcctgagc tcgtcgcgga tttccgcgag
     1621 cagctcgact tcgctcggcc cggccggggc gtcggcgccg cggggggtca gccggttggc
     1681 ccagcgcacc agcaggaaga tgacgaaggc gagaatgacg aagttgatca gcacggtgac
     1741 gaaccggccc cagccgagca ccgcgacgcc ggccttcttg aggtccgcgt agcttgcaag
     1801 gctgcccgcg taattgtcgg gcaccgggcc gaggatggtg aaataattgg cgaaatcgag
     1861 cccgccgaag atcgctccga cgaccggcat gatgatgtcg tcggtgaggc tcgcggtgat
     1921 ggtcgcgaac gcggcgccga tgatcaccgc gacggcaagg tcgaggacgt tgccgcgggc
     1981 cacaaaatcg cgaaattcca tgagcattat ctttcccctc acgcctgccg gtccatccta
     2041 gccggcgcag cgctgattga aagcgggctc gcgcccctct acttatcgcc gtcacatcat
     2101 gtgatcaggg gatttcgaga tgaccgcact ccgccgtttt gggttgcttg caccgctcgc
     2161 cgcgttcagc ctcgccggct gcgggctcaa caccgtcccg acggccgagg agcaggtcaa
     2221 cgccgcctgg ggcgatgtcc agagcgagta tcagcggcgc gccgacctca tccccaacct
     2281 cgtccggacg gtcgagcgct acgccgacca ggagcgcgac gtgctgaccg aggtgaccaa
     2341 cgcccgcgcc cgggcgtcga gcatccagct tcgcgccgag gacctcgacg accccgccaa
     2401 ggtgcgcgcg ttcaacgatg cgcagaccca gctgggcggc tcgctggggc ggctgctggc
     2461 gacgttcgag gcctatccgg acctcaagtc gaaccagaac ttcctgaccc tgcagagcca
     2521 gctcgagggc accgagaatt cgatcctcgt ttcgcgccgc gactacaatg aggcggtccg
     2581 gcagtacaac acgaccatcc gaaccttccc cgatgcgatc ggcgccaaga tcttctacgg
     2641 cgccaagccc aaggtgccgt tcgaggcggc gaccggagcg caaaccgcgc cgacggtcga
     2701 tttcaacgcc agctagggtg acgcgacttt tcgccgtctt gtggctggcg ctgatcgcgc
     2761 tggccgcacc ggctgcggcg cagacgttcc cgcagctgac cggccgcgtc gtcgaccagg
     2821 cggacctgct ccggcccgag caggaactcg acctgtcgag caagtcggcg gcgctcgagg
     2881 cccagaccgg gcgccagttc gtcgtcgcca ccgtcaacag cctcgaggga cggaccatcg
     2941 aggactatgg ctaccggctc ggccggcact ggaagatcgg cgacgagaag cgcgacgacg
     3001 gggtgctgct gctggtcgcg cccaacgaga agaaggtgcg gatcgagacc ggctacgggg
     3061 cccgcgtgtt cctgaccgac gcggtgtcga gcgtcatcat ccgcgaatcg atcctgccgc
     3121 gcttcaaggc cggcgacatg ggcggcggga tcaccgccgg cgccgaccag atcgtcaaga
     3181 tgatgagcct gtcgccgcag gaagcgcagc gccaggcggg cgcggccgag cgccgcgagg
     3241 cgcagcgggc cgagtccggg cccggcctga tcccggtcgt cttctgggtg atgatcgcg
//
LOCUS       DASTZO010000286         3783 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_963904,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000286 DASTZO010000000
VERSION     DASTZO010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3783
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963904"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1179
                     /locus_tag="VFU20_08045"
     CDS             <1..1179
                     /locus_tag="VFU20_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HEU5286441.1"
                     /translation="TSGTGGAPRGVRQHHGAILHNIAGCVDVIAEDFGWDDEVFLSFL
                     PASHAYEHTGGQHFPVGLGAQIYYAESLEKLAANIEEVRPTIMVVVPRLFEMLRARIL
                     KTISKGSLSEYLLNRALAIGSSRYEGRFKPWDLPMDGLLSLTLRRKVAGRFGGRVKAM
                     VSGGAPLNPEVGLFFHSLGLTLLQGYGQTEAGPVISCNRPSAGIRMDTVGPPLKDTEV
                     KIAEDGEILVRGENVMHGYWQSPEETARVLHNGWLATGDVGYLDDKGRIVITDRKKDL
                     IVNDKGDNVSPQRVEGMLTLQPEIAQAMVYGDRRPYLVALLVPDPEYAGQPDVEQRLK
                     AAVDRVNADLSIVEKVRRFITADGPFTIDNEQLTPSMKIRRHVLKAVYAERLDALYRK
                     "
     gene            complement(1195..1419)
                     /locus_tag="VFU20_08050"
     CDS             complement(1195..1419)
                     /locus_tag="VFU20_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286442.1"
                     /translation="MTGILDKFHVEHGIHGARVMARSLQLTGDLTADREVDTAIRMLK
                     DDLDACAKEMKRLIDVNSRGSMFAGWPTGV"
     gene            1598..1978
                     /locus_tag="VFU20_08055"
     CDS             1598..1978
                     /locus_tag="VFU20_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286443.1"
                     /translation="MSTEQKMVRIIALVAIALGSPAVAAPQASSAAADAKPPVVCKYV
                     VTAEAGSKAFRMCLTKSEWAQREAQASKDANRIECRFQEVPGTRFRSKKICQPASAWA
                     EHRRLEREAIEQIQRSVCVYGAGC"
     gene            complement(2103..2510)
                     /locus_tag="VFU20_08060"
     CDS             complement(2103..2510)
                     /locus_tag="VFU20_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6491 family protein"
                     /protein_id="HEU5286444.1"
                     /translation="MRGIALLMIGATLASCAAAPPAGRSADAERHFQKLIAGKVAGQP
                     VSCLPTYRSNDMVVIDDQTVAFRDGSRRVWVTDLGQGCANLGSGFYALVTRRHGGTGL
                     CRGDIAHVADLTSGFTVGSCVMGDFTPYVRPRG"
     gene            complement(2558..3289)
                     /gene="gloB"
                     /locus_tag="VFU20_08065"
     CDS             complement(2558..3289)
                     /gene="gloB"
                     /locus_tag="VFU20_08065"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006955650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HEU5286445.1"
                     /translation="MPAPLEIVAVPAFTDNYIWLVHDPASGETAVVDPGDAAPALAEV
                     ERRGWTVGQVWNTHHHWDHSGGNLAMKQATGCTVAGPAAEAIPGRDVALSEGSELGLG
                     SHRGRVIEIPGHTLGHVALIFDEAKVAFVGDTLFAMGCGRLFEGTAEQMHRSLQRLAA
                     LAPDTALYCGHEYTLANARFAIHAEPGNPDIAARLERVQAVRAAGEITLPTSVAEERA
                     TNPFVRAAGVGEFARLRAEKDSFRS"
     gene            complement(3289..>3783)
                     /locus_tag="VFU20_08070"
     CDS             complement(3289..>3783)
                     /locus_tag="VFU20_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286446.1"
                     /translation="MWIAAGQPGKAALALDRALAGTALQAEQRGEALLDRARAAEAQG
                     DLKTARAKVTEAAATISADPFYWYFSAALAIREEDKATAQYAIGRALGLAPTDPTILF
                     EAGHVAHFAGDPAKARSYWSQANHFDPAGPIGKAAREALRLLSAPGRDTAPVTQRAAP
                     AKSN"
BASE COUNT          598 a         1295 c         1301 g          589 t
ORIGIN      
        1 accagcggca ccggcggcgc cccgcgcggg gtccgccagc accacggcgc gatcctccac
       61 aacatcgccg gctgcgtcga cgtcatcgcc gaggatttcg gctgggacga cgaagtgttc
      121 ctgtcgttcc tgcccgccag ccacgcctac gagcacaccg gcggccagca tttcccggtc
      181 gggctcgggg cgcaaatcta ttacgccgaa agcctcgaga agctcgccgc caacatcgag
      241 gaggtgcggc cgacgatcat ggtcgtcgtc ccgcggctgt tcgagatgct gcgcgcgcgg
      301 atcctcaaga ccatctcgaa gggcagcctg tccgaatatc tgctcaaccg cgccctggcg
      361 atcggctcga gccgctacga aggccggttc aagccgtggg acctgccgat ggacggcctg
      421 ctgtcgctga ctcttcgccg caaggtcgcc gggcgcttcg gcgggcgcgt gaaggcgatg
      481 gtctcgggcg gcgccccgct caaccccgag gtcggtctgt tcttccattc gctcgggctg
      541 accctgctgc agggctacgg ccagaccgag gccggcccgg tgattagctg caaccggccg
      601 agcgccggga tccgcatgga cacggtcggg ccgccgctca aggacaccga ggtgaagatc
      661 gccgaggacg gcgagatcct ggtccgcggc gagaatgtga tgcacggcta ttggcagagc
      721 cccgaggaaa ccgctcgggt gctccacaac ggttggctcg ccaccggcga cgtgggctat
      781 ctcgacgaca agggccggat cgtcatcacc gaccgcaaga aggatttgat cgtcaacgac
      841 aagggcgaca acgtctcgcc gcagcgggtc gaggggatgc tgaccctgca gcccgagatc
      901 gcccaggcga tggtctacgg cgaccgccgc ccgtacctcg tcgcgctgct ggtgcccgat
      961 cccgaatacg ccggccagcc cgacgtcgag cagcgcctca aggcggcggt cgatcgggtc
     1021 aacgccgacc tgtcgatcgt cgagaaggtg cgccgcttca tcaccgccga cgggccgttc
     1081 acgatcgaca acgagcaatt gaccccgtcg atgaagatcc gccgccacgt gctcaaggcc
     1141 gtgtacgccg agcggctcga cgcgctgtac cggaagtagg accgttgccg gccttcagac
     1201 gcccgtcggc cagcccgcga acatggagcc gcgcgaattg acgtcgatca gacgcttcat
     1261 ctctttcgcg caggcgtcga gatcgtcctt gagcatccgg atggcggtgt cgacctcgcg
     1321 gtccgcggtg aggtcgccgg tgagctgaag cgagcgcgcc atcaccctcg ccccgtggat
     1381 cccatgttcg acgtggaact tgtcgagaat gccggtcatg agcgcctcca tgcttcgcgc
     1441 cagcaatagc atgagcagcg gctcttgccg cgtcaaaatt cacgccgcga cggatggcgg
     1501 acggccgccg tgggtggaaa gcggacattt ggccgggtat gtggaacagg tctggcggtt
     1561 ccctttctgg gcctgatcct cagtgaggag ccaccgaatg agtacggagc agaagatggt
     1621 caggatcatc gctttggtgg ccattgccct gggatcgccg gcagtcgccg cgccgcaggc
     1681 atcgtcagcc gcggcggacg ccaagccacc ggtcgtctgc aaatacgtcg ttacggcgga
     1741 ggccggttca aaggcgttcc ggatgtgcct gacgaagagc gagtgggccc agcgcgaggc
     1801 ccaagccagc aaggacgcaa accggatcga atgtcgcttc caggaggtgc cgggcacccg
     1861 attcagatcc aagaagatct gccagcccgc atccgcctgg gccgagcacc ggcgcctcga
     1921 gcgcgaggcg atcgaacaga ttcagaggag cgtctgcgtc tacggagccg gctgctgagc
     1981 gaccctcttt cgcccgcagt tcgttgagcc gcagccacca gcgttgccac ctgtgaagcc
     2041 cgaaacgacc gtaatctccg cgatgggctg gaagcggacc tttgcggaag gcggcggctg
     2101 cctcagcccc gcggcctcac gtatggcgtg aagtcgccca tcacgcagct gccgacggtg
     2161 aagccgctgg tcaggtcggc gacgtgggcg atgtcgcccc ggcacaggcc ggtgccgccg
     2221 tggcgcctgg tgaccaaagc ataaaagccc gagccgaggt tcgcgcagcc ctggccgagg
     2281 tcggtcaccc acacccggcg gctgccgtcg cggaaggcca ccgtctggtc gtcgatcacc
     2341 accatgtcgt tgcttcggta agtcggcagg cagctgaccg gctggccggc gaccttgccg
     2401 gcgatcagct tctggaaatg gcgctccgcg tcggcgcttc ggccggccgg gggcgcggcg
     2461 gcgcaggacg cgagcgtcgc gccgatcata aggagagcaa tgccacgcat gacgacctcc
     2521 ttttcaatgc gtcttaaaga cgcattgtcg cctgcgctca tgaacggaag ctgtctttct
     2581 ccgcccgcag gcgcgcgaat tcccccacgc ccgcagctcg aacgaagggg ttggtcgccc
     2641 gttcctcggc gacgctggtc ggaagcgtga tttcgccggc ggcgcgcacc gcctgcaccc
     2701 gctccagccg cgccgcgatg tccgggttgc cgggctcggc gtggatcgcg aagcgcgcgt
     2761 tggcgagcgt atattcgtgc ccgcagtaga gcgcggtgtc cggcgccagc gccgccagcc
     2821 gctgcagcga gcggtgcatc tgctccgcgg tgccctcgaa cagccgcccg cagcccatcg
     2881 cgaacagggt atcgccgacg aacgcgacct tcgcctcgtc gaagatcagc gcgacgtggc
     2941 cgagcgtgtg gccggggatt tcgatcaccc ggccccgatg gctgccgagg ccgagctcgc
     3001 tgccttcgga caaagcgacg tcgcggccgg ggatcgcctc ggcggcgggg ccggcgaccg
     3061 tgcagccggt cgcctgcttc atcgccagat tgccgccgct gtggtcccag tgatgatgcg
     3121 tgttccacac ctggccgacc gtccagccgc ggcgctccac ctcggccagc gccggcgccg
     3181 cgtcgcccgg atcgaccacc gccgtctcgc cgctcgctgg gtcgtggacc agccagatgt
     3241 aattgtcggt gaaggccggg acggcgacga tctcgagcgg cgcgggcatc agttgctttt
     3301 agccggcgcc gccctttgcg tcacgggtgc cgtgtccctg ccgggagcgg acaatagcct
     3361 gagcgcctcg cgcgccgcct tgccgatcgg ccccgcgggg tcgaaatggt tggcctggct
     3421 ccagtaggag cgcgccttgg ccgggtcccc ggcgaaatgg gcgacatggc cggcctcgaa
     3481 caggatcgtc gggtcggtcg gggcgaggcc gagcgcccgg ccgatcgcat attgggcggt
     3541 ggccttgtcc tcctcgcgga tcgccagagc ggccgagaaa taccagtaga aggggtcggc
     3601 ggagatggtc gcggcggcct cggtcacttt cgcgcgcgcg gtcttgaggt cgccctgcgc
     3661 ctcggcggcg cgggcgcggt cgagcagcgc ctcgccgcgc tgctcggcct gaagcgcggt
     3721 gccggccagc gcccggtcga gcgccagcgc ggccttgccc ggctggcccg cggcgatcca
     3781 cat
//
LOCUS       DASTZO010000287         4700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_44179,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000287 DASTZO010000000
VERSION     DASTZO010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4700
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44179"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            86..586
                     /locus_tag="VFU20_08075"
     CDS             86..586
                     /locus_tag="VFU20_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286447.1"
                     /translation="MDSRTALLIIAATALPASPPAAAARAQTLFAQGQWAALRFGAQC
                     EAVARPLLPASRRQPESRAGFAFHPAGNRIGEFHARLSRAPRPGSSVMLIVGERPFLL
                     VARGDRAWSRGPAQDWAIIAAARAATSMRVEARDGAGRRFADRYLLAGAPTAIDAAAA
                     ACAGKI"
     gene            614..1777
                     /gene="rlmN"
                     /locus_tag="VFU20_08080"
     CDS             614..1777
                     /gene="rlmN"
                     /locus_tag="VFU20_08080"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019516746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU5286448.1"
                     /translation="MSAATALMPIPGVIDPVTVRRGPNPRADGRTELIGLSKEAIRAA
                     LEAEGLDARQAKLRSKQLWHWIYNRGVSDFAAMSDIAKAQAPWLAERFVISRPQVVEA
                     QVSSDGTRKWLLRTHDGNDFEMVFIPESDRGTLCVSSQVGCTLNCTFCHTGTMRLVRN
                     LEPSEIVGQVMLARDALGEWPTQPEGRMLTNIVMMGMGEPLYNFDNVRDALKIVMDGD
                     GLGLSKRRITLSTSGVVPMMARAGEEIGVNLAVSLHSVRKEIRDEIVPLNRKYGIEQL
                     LEACAAYPGANNARRITFEYVMLKDKNDSDADALELVRLIRRYKLPAKVNLIPFNPWP
                     GAPYECSSPERIKAFSSIVFEAGISAPVRTPRGRDIDAACGQLKTASERKRRA"
     gene            1793..3016
                     /locus_tag="VFU20_08085"
     CDS             1793..3016
                     /locus_tag="VFU20_08085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007687761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEU5286449.1"
                     /translation="MALAGRLIERLLKRGQITVVTADGKRATYGPGGGKSLTIRFRDR
                     KVPFELVRNPRLGLGEAYMDGRLLIEGGSMLDLLEMITASNRWEEGGKGKILPKGRIA
                     AARAFFRRNDPRRARRNVAHHYDLSDELYETFLDPDLQYSCAYYTSPGNSLEQAQADK
                     KAHIAAKLGLKAGHRVLDIGCGWGGMALYLHRVAGVEVLGITLSEHQLAVARRRAEEA
                     GVADHVRFELIDYRLVDDRFDRIVSVGMFEHVGLAHYDEFFAKCRELLKPDGVMLLHT
                     IGKMGTASASPDPFTDKYIFPGYHLPSLSQICAASEKARLVASDVETLRLHYAYTLRE
                     WLRRFTAARPRMVELYDERFFRMWEYYLVGGIVMFENGGGCNYQIQYIRDRKTLPITR
                     DYMAKAEARYRQQET"
     gene            complement(3071..3925)
                     /locus_tag="VFU20_08090"
     CDS             complement(3071..3925)
                     /locus_tag="VFU20_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-hand domain-containing protein"
                     /protein_id="HEU5286450.1"
                     /translation="MPAIGFPSRLDCGGRALATPAPAIQARYSLPGIPRPRSFTEGVS
                     DMSKYLLAGAAVAAIALSPAIAQSVQPAPAPAPAPQALIHHAPIAKVHSRAEVGAHVR
                     AMFERLDTNRDGFVTKAEAQAARAHRADRVIKRIRHGAAGSPEQRLADRGAMFDRIDT
                     NRDGSISREEFAKVRENRVERRIVMREKLKEGEAAPSDGKGGVSHNGAMGLMQHPGGY
                     DARRGGLGGRMFDMADANKDGRVTLQEATAAAYRHFDMADANRDGQVTREERMQMRQK
                     MRAERRPG"
     gene            3915..>4700
                     /locus_tag="VFU20_08095"
     CDS             3915..>4700
                     /locus_tag="VFU20_08095"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017183964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU5286451.1"
                     /translation="MAGKIDRVVLAFSGGLDTSVILKWLQQQYACEVVTFTADLGQGE
                     ELEPARAKAELLGVRPEHIFIEDLREEFVRDFVFPMMRANALYEGAYLLGTAIARPLI
                     AKRQVEIAREVGADAVAHGATGKGNDQLRFEFGYYALAPDIKVIAPWREWDLTSRAAL
                     IDFAEKHQIPVAKDKRGESPFSTDANLLHTSSEGKVLEDPWAEVPDYVYSRTDDPRTA
                     PDEPEDVIVGFERGDAVAVNGEPLSPAALLERLNALGKAHGVGR"
BASE COUNT          748 a         1628 c         1592 g          732 t
ORIGIN      
        1 aacaagttaa tggagcaaat aatttccaga ttggagcgat ttagctcgct ggcgccctgg
       61 gccgccggcg cgctatcgtt cgccgatgga cagccggacg gcacttttga tcatcgccgc
      121 gacggccttg ccggcgtcgc cgccggcagc cgccgcccgg gcgcaaaccc tgttcgcgca
      181 aggccagtgg gcggcgctcc ggttcggcgc ccagtgcgag gcggtcgccc ggccgctgct
      241 tccggcgtct cggcgccagc ccgaatcgcg cgccggcttc gccttccatc ccgccggcaa
      301 ccggatcggc gagttccacg cgcgcctgag ccgggccccg cgccccggct cgagcgtcat
      361 gctgatcgtc ggcgagcgcc cgttcctgct ggtagcgcgc ggcgaccgcg cgtggagccg
      421 cggcccggcg caggattggg cgatcatcgc cgcggcgcgg gcggcgacat cgatgcgggt
      481 cgaagcgcgc gacggcgccg ggcgccgctt cgccgaccgc tacctgcttg ccggagcgcc
      541 gaccgcgatc gacgccgccg cggccgcctg cgctggcaaa atctagccgg cgcccctaga
      601 tgggccgcct gcaatgagcg ccgccaccgc cctgatgccg atccccggcg tgatcgatcc
      661 cgtgaccgtg cggcgcgggc cgaacccgcg cgccgacggc cgcaccgagc tcatcggcct
      721 gtcgaaggag gcgatccgcg ccgcacttga ggccgagggc ctcgatgcgc gccaggcgaa
      781 gctccgctcc aagcaattgt ggcactggat ctacaaccgc ggggtcagcg actttgcggc
      841 gatgagcgac atcgccaagg cgcaggcgcc gtggctcgcc gagcgcttcg tcatttcgcg
      901 gccgcaggtg gtcgaggcgc aggtgtcgtc cgacggcacc cgcaaatggc tgctccggac
      961 ccacgacggc aacgatttcg agatggtgtt catccccgaa tccgaccgcg gcaccttgtg
     1021 cgtgtcgagc caggtcggct gcacgctcaa ctgcaccttc tgccacaccg ggacgatgcg
     1081 gctggtgcgc aacctcgagc cgtcggagat cgtcggccag gtgatgctcg cccgcgacgc
     1141 gttgggcgaa tggccgaccc agccggaagg ccggatgctg accaacatcg tcatgatggg
     1201 catgggcgag ccgctgtaca atttcgacaa tgtccgcgac gcattgaaga tcgtcatgga
     1261 cggcgacggc ctcggcctct ccaagcgccg gatcactctg tcgaccagcg gcgtggtgcc
     1321 gatgatggcg cgcgccggcg aggagatcgg ggtcaacctc gcggtgtcgc tgcactcggt
     1381 ccgcaaggaa atccgcgacg agatcgtgcc cttgaaccgc aagtacggga tcgagcaatt
     1441 gctcgaggcc tgcgcggctt atccgggcgc caacaacgcg cgccggatca cgttcgaata
     1501 tgtgatgctg aaggacaaga acgacagcga cgcggacgcg ctcgagctgg ttcggctgat
     1561 ccgcaggtac aagctgccgg cgaaggtcaa tctgatcccg ttcaacccgt ggccgggcgc
     1621 gccctacgaa tgctcgtcgc ccgaacggat caaggccttt tcgagcatcg tcttcgaggc
     1681 cggcatctcg gccccggtgc ggaccccgcg cggccgcgac atcgacgccg cctgcgggca
     1741 gttgaagacc gcttcggagc gcaagcgccg cgcataaggg aggccccggg gaatggcatt
     1801 ggccggcagg ttgatcgagc ggctgctcaa gcgcggccaa atcacagtcg tcaccgccga
     1861 cggcaagcgc gccacctacg ggcccggcgg cggcaagtcg ctgaccatcc gcttcagaga
     1921 ccgcaaggtg ccgttcgagc tcgtccgcaa cccgcggctg ggcctcggcg aagcctatat
     1981 ggacggccgc ctgctgatcg agggcggcag catgctcgac ctgctcgaga tgatcaccgc
     2041 gtccaaccgg tgggaggagg gcggcaaggg caagatcctg cccaagggca ggatcgccgc
     2101 cgccagggcc ttcttccgcc gcaacgaccc gcgccgggcg cggcgcaacg tcgcccacca
     2161 ttacgacctc agcgacgagc tctacgaaac cttcctcgac cccgaccttc aatacagctg
     2221 cgcttattac accagccccg gcaacagcct cgaacaggcg caggccgaca agaaggcgca
     2281 catcgccgcc aagctcggac tgaaggccgg gcaccgggtg ctcgacatcg gctgcggctg
     2341 gggcgggatg gcgctctacc tccaccgggt cgccggggtc gaggtgctcg gcatcaccct
     2401 gtccgagcac cagctggcgg tcgcccgccg ccgcgccgag gaagccggcg tcgccgacca
     2461 cgtccgcttc gagctgattg attaccggct ggtcgacgac cggttcgacc gaatcgtctc
     2521 ggtcggcatg ttcgagcatg tcggcctcgc ccattacgac gagttcttcg ccaagtgccg
     2581 cgaattgctg aagcccgacg gggtgatgct gctgcacacc atcggcaaga tgggaacggc
     2641 gtcggcttcc ccggacccgt tcaccgacaa atatatcttc cccggctatc atctgccgtc
     2701 gctgtcgcag atctgcgcgg cgagcgagaa agcgcggctg gtcgccagcg acgtcgagac
     2761 gcttcggctg cactacgcct acacgctgcg cgaatggctg cggcgcttca ccgcggcgcg
     2821 cccgcgtatg gtcgagctct acgacgagcg cttcttcagg atgtgggaat attatctggt
     2881 cggcggcatc gtcatgttcg agaacggcgg cggctgcaat taccagatcc agtacatccg
     2941 cgaccgcaag acgctgccga tcacgcgcga ttacatggcc aaggccgagg cgcggtaccg
     3001 ccagcaagag acgtagaaaa aagcccggcg gtttcccgcc gggcctcgtt ggcgatcact
     3061 cgatgggggc ctagcccggg cggcgctcgg cgcgcatctt ctggcgcatc tgcatccgct
     3121 cctcgcgcgt cacctggccg tcgcggttgg catcggccat gtcgaaatgc cggtaggcgg
     3181 cggcggtcgc ttcctgcagg gtgacgcggc cgtccttgtt ggcgtcggcc atgtcgaaca
     3241 tgcggccacc gagtccgcct ctgcgggcat cgtagccgcc ggggtgctgc atgaggccca
     3301 tggcaccgtt atggcttact ccgcccttgc catcactggg cgctgcttcg ccttccttca
     3361 gtttctcgcg catgacgata cggcgctcga cccggttctc gcggaccttg gcgaactctt
     3421 cgcggctgat cgagccgtcg cggttggtgt cgatgcggtc gaacatcgcg ccgcggtccg
     3481 ccaggcgctg ctcgggcgag ccggcggcgc cgtgccgaat gcgcttgatc acgcgatcgg
     3541 cgcgatgggc ccgggccgcc tgcgcctcgg ccttggtcac aaaaccgtcg cggttggtat
     3601 cgagccgctc gaacatcgcg cggacgtggg ctccgacttc ggcgcggcta tgaaccttgg
     3661 cgatcggcgc gtggtggatc agcgcctgcg gcgccggcgc gggcgcgggc gccggctgga
     3721 ccgattgcgc gatggccggt gacagcgcga tcgccgcgac ggcggctccg gcgagaagat
     3781 atttgctcat gtcgctcact ccctccgtga aactccgcgg ccggggtatg cccggcaagc
     3841 tgtatcgcgc ttgaatggcc ggggcggggg tggcaagggc gcgtccgccg caatccagcc
     3901 gcgaagggaa gccgatggcc ggcaagatcg accgcgtcgt cctcgctttc tccggcgggc
     3961 tcgacaccag cgtcatcctc aaatggctgc agcagcaata tgcctgcgag gtcgtcacct
     4021 tcaccgccga cctcggccag ggcgaggagc tcgagccggc gcgcgccaag gccgagctgc
     4081 tcggcgttcg ccccgagcac attttcatcg aggatctgcg cgaggagttc gtgcgcgact
     4141 tcgtcttccc gatgatgcgc gccaacgcgc tctacgaggg cgcctatctg ctcggcaccg
     4201 cgatcgcccg gccgctgatc gccaagcgcc aggtcgagat cgcgcgcgag gtcggcgccg
     4261 acgcggtcgc ccacggggcg accggaaagg gcaacgacca gctgcgcttc gagttcggct
     4321 attatgcgct ggcgcccgac atcaaggtga tcgccccgtg gcgcgaatgg gacctcacca
     4381 gccgggccgc gctgatcgac ttcgccgaga agcaccagat cccggtggcg aaggacaagc
     4441 gcggcgaaag cccgttctcg accgacgcca atttgctcca cacgtcgagc gagggcaaag
     4501 tcctcgagga cccgtgggcc gaggtcccgg attacgtcta ttcgcgcacc gacgacccgc
     4561 ggaccgcgcc cgacgagccc gaggacgtga tcgtcgggtt cgagcgcggc gacgcggtcg
     4621 cggtcaacgg tgagccgctg tcgccggcgg ctttgctcga acggctgaac gcgctcggca
     4681 aggctcacgg cgtcggccgc
//
LOCUS       DASTZO010000288         4772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_624607,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000288 DASTZO010000000
VERSION     DASTZO010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624607"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..549)
                     /gene="ccsA"
                     /locus_tag="VFU20_08100"
     CDS             complement(<1..549)
                     /gene="ccsA"
                     /locus_tag="VFU20_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU5286452.1"
                     /translation="MIAEAGLAALWLAAALALLQLLLTLGELRGWYDGARAIRSVAIV
                     QGLLTLVAFAMLVLVFVRTDLSVALVAANSHSAKPMIYKLVGAWGNHEGSMLLWVTVL
                     ALAGALLAAASRRLAERTLIAALGAQGALALGFYAFLLIASNPFARLNPAPIEGRGLN
                     PLLQDPGLAFHPPTLYLGYVGLS"
     gene            complement(559..999)
                     /gene="ccmE"
                     /locus_tag="VFU20_08105"
     CDS             complement(559..999)
                     /gene="ccmE"
                     /locus_tag="VFU20_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019368073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HEU5286453.1"
                     /translation="MKPKTQRLALVIAAAVALLAAVLLAMWGLRDRAAYFYTPADIAA
                     GRAIENKAMRLGGMVETGSVQRQADGVTIRFAVVDGEARTPVTYRGIVPDLFREGSGV
                     VAEGRLSQGTFVADTILAKHDERYMPPQLGNQAAEHKAAKTLER"
     gene            complement(996..1121)
                     /gene="ccmD"
                     /locus_tag="VFU20_08110"
     CDS             complement(996..1121)
                     /gene="ccmD"
                     /locus_tag="VFU20_08110"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008996848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HEU5286454.1"
                     /translation="MNPWPFVIAAYAVTVIAVAGLVWWARASMRRAEAAADKLRQ"
     gene            complement(1118..1834)
                     /gene="ccmC"
                     /locus_tag="VFU20_08115"
     CDS             complement(1118..1834)
                     /gene="ccmC"
                     /locus_tag="VFU20_08115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease CcmC"
                     /protein_id="HEU5286455.1"
                     /translation="MHFLANPARFLRIAKPATAWLMAAGLLLTLAGMAAGLTLTPPDY
                     LQGETVRILYVHVPAAWLGMAGWAGIAAASVSQLVWRHPLAAIAGRAIAPAGATFAGI
                     CLATGSIWGRPTWGTWWEWDGRLTSMLVLFFLYLGYIALAAAEKERGGEGRLAAIFGL
                     VGAINLPVIHYSVLWWRTLHQGQSIGATGSSIDTSILWPLPLTMVGLTAVFAGVALMR
                     MRAELAEAKVEARLRRAAAA"
     gene            complement(1931..2977)
                     /locus_tag="VFU20_08120"
     CDS             complement(1931..2977)
                     /locus_tag="VFU20_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012050170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEU5286456.1"
                     /translation="MTCPWRLPDFDAHEVLHFAHDSATGLQAIIAIHSTHLGPSAGGC
                     RFWHYAEDSDAVTDALRLSRGMSYKNAMAGLPLGGGKSVILADEKGTKTPELLAAFGR
                     AVDRLGGRYVTAEDVGMSVKDMVEVSRQTKYVAGLPAAEGVGGDPGPHTALGVFLGIK
                     AAVRQALGKDSVSDLHIALQGAGSVATGVALHAAAEGARLSVADLDPDKAQKLAAAAD
                     GKVVAPEEILSLEADVVSPCALGAILDETTIPALRAPIVAGGANNQLATPADGARLHQ
                     RGILYAPDYVINAGGIINVCTEYLGDGDETLVRQRIEGIPDRLGQIWAESASSGRDPA
                     AVADAMAQRLIGRG"
     gene            complement(3014..3090)
                     /locus_tag="VFU20_08125"
     tRNA            complement(3014..3090)
                     /locus_tag="VFU20_08125"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3054..3056),aa:His,seq:gtg)
     gene            3203..3628
                     /locus_tag="VFU20_08130"
     CDS             3203..3628
                     /locus_tag="VFU20_08130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009822299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEU5286457.1"
                     /translation="MNATIRKFGHPASLVREFEHWLVLVRPAQATLGSLVLAAKSDAA
                     AFRELPRAAFAEQADAVAAIETGLAAFCRFERINYLMLMMVDRHVHFHVVPRYSGSRE
                     WNGLDFADRGWPGPPDLNSAIHLSASQVRDLRDDLAKLF"
     gene            3702..4415
                     /locus_tag="VFU20_08135"
     CDS             3702..4415
                     /locus_tag="VFU20_08135"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5286458.1"
                     /translation="MQASRRSAAAPAVSLGDTLTAAGLDRRLATLFEEMPHKIVEVDP
                     RRPFRDPGAVRDEFIFVRSGILCKYKTDGGGRRQIVALRFPGEAILPREGPAGYGLQG
                     IVKSEVMVGQASDFDAIVDSNPELRRFFWRLIQRNEAIGYEWLVNCGRRDSTARVAHL
                     LCETAARSGISGEHGRMLNPFTQQQIADITGQTSVNVNRVLADLQRQGLIERSGREIA
                     FPDWAELRRIASFQPAYLE"
     gene            complement(4468..4542)
                     /locus_tag="VFU20_08140"
     tRNA            complement(4468..4542)
                     /locus_tag="VFU20_08140"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4508..4510),aa:Val,seq:cac)
     gene            complement(4643..4717)
                     /locus_tag="VFU20_08145"
     tRNA            complement(4643..4717)
                     /locus_tag="VFU20_08145"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4683..4685),aa:Val,seq:cac)
BASE COUNT          756 a         1653 c         1670 g          693 t
ORIGIN      
        1 cgacaggccg acgtagccca gatagagcgt cggcgggtgg aaggcgaggc cggggtcctg
       61 gagcaagggg ttgagccccc ggccctcgat cggcgccggg ttcagccggg cgaacgggtt
      121 cgaggcgatc agcaggaacg cgtagaagcc gagcgccagc gcgccctgcg ccccgagcgc
      181 cgcgatgagc gtgcgctcgg cgaggcggcg cgaggccgcg gccagcagcg ccccggcgag
      241 cgcgagcacc gtcacccaca gcagcatcga gccctcgtgg ttcccccacg cgcccaccag
      301 tttgtagatc atcggcttgg cgctgtggct gttggccgcg accagcgcga ccgacaggtc
      361 ggtccggacg aagaccagga ccagcatcgc gaatgcgacc agggtcagca ggccctggac
      421 gatggcgacc gagcggatcg cgcgcgcccc gtcgtaccag ccgcgcagct cgcccagagt
      481 cagcagcagc tggaggagcg ccaacgcggc cgccagccac aatgcggcga gacccgcttc
      541 agcgatcatt ttgggcgatc atcgctcgag cgtcttcgcc gccttgtgct ccgcggcctg
      601 gttgccgagc tgcggcggca tgtagcgctc gtcgtgcttg gcgaggatcg tgtcggcaac
      661 gaaggtgcct tggctgaggc gcccttccgc gaccaccccg ctgccttcgc ggaacaggtc
      721 ggggacgatg ccacggtagg tgaccggcgt gcgcgcctcg ccgtcaacga cggcgaagcg
      781 gatggtgacc ccgtcagcct gcctttgcac cgaaccggtc tcgaccatcc cgccgagccg
      841 catcgccttg ttttcgattg cccggccggc ggcgatgtcg gcgggagtgt agaaataagc
      901 agcgcggtcc ctgaggcccc acattgccag cagcaccgcg gcgagcaggg cgacggccgc
      961 ggcgatcacc agggccagcc gctgggtctt gggcttcatt ggcgcagctt gtcggccgcc
     1021 gcctcggcgc gccgcatcga cgcgcgcgcc caccagacca ggccggcgac ggcgatcacg
     1081 gtcaccgcat aagcggcgat gacgaacggc cacgggttca tgccgcggcc gcccggcgga
     1141 ggcgcgcctc gaccttggcc tcggcgagct cggcccgcat gcgcatcagc gccactccgg
     1201 cgaagaccgc ggtcagcccg accatggtca gcggcagcgg ccacaggatc gaagtgtcga
     1261 tgctcgagcc ggtggctccg atgctctggc cctggtggag cgtgcgccac cacaggaccg
     1321 aataatggat gaccggcagg ttgatcgcgc cgacgaggcc gaagatcgcc gccagccgcc
     1381 cttccccgcc gcgctctttc tccgctgctg cgagcgcgat gtagccgagg tacaggaaga
     1441 acaggaccag catcgaggtc agccgcccgt cccattccca ccaggtgccc caggtcggcc
     1501 ggccccagat cgagccggtc gcgaggcaga ttcccgcgaa ggtcgctccg gccggagcga
     1561 tcgcccggcc ggcgattgcg gcgagcgggt ggcgccagac caattggcta accgaggcgg
     1621 cggcgatccc ggcccagccg gccatgccga gccacgccgc agggacgtgg acgtagagga
     1681 tccgcacggt ctcgccctgg aggtagtcgg gcggcgtcag ggtcaggccg gcggccattc
     1741 cggcgagcgt cagcaacagc ccggccgcca tcagccaggc ggtcgcgggc ttggcgatgc
     1801 gcaggaagcg ggcgggattg gcgaggaagt gcatcgtggc gctactagca cgtcattgcg
     1861 aggagcgaag cgacgcggca atccaagcaa gaacaaaact ggattgccgc gcttcgctcg
     1921 caatgacggc tcagccccgg ccgatcagcc gctgcgccat cgcatcggcg accgcggccg
     1981 ggtcgcggcc cgacgaggcg ctctcggccc agatctgccc gagccggtcg gggatgccct
     2041 cgatccgctg gcggacgagg gtctcgtcgc cgtcgccgag atattcggtg cagacgttga
     2101 tgattccgcc ggcgttgatc acataatcgg gcgcgtagag gatcccgcgc tggtgaagcc
     2161 gggctccgtc cgcgggggtc gcgagctggt tgttggcgcc cccggcgacg atcggcgcgc
     2221 gcagcgccgg gatggtcgtc tcgtcgagga tcgctccgag cgcgcagggg ctgacgacgt
     2281 cggcctcgag cgacaggatt tcctcgggag cgacgacctt gccgtcggcg gcggcggcga
     2341 gcttctgcgc cttgtcgggg tcgaggtcgg cgaccgacag cctcgcgcct tcggccgcgg
     2401 cgtggagcgc gaccccggtc gcgacgctgc ccgcgccctg cagggcgatg tgcaggtcgg
     2461 acaccgaatc cttgccgagc gcctggcgga ccgcggcctt gatgccgagg aacacgccga
     2521 gcgcggtgtg cggcccggga tcgccgccga ccccttccgc ggccgggagc ccggcgacat
     2581 atttggtctg gcggctgacc tcgaccatgt ccttgacgct catcccgacg tcctcggcgg
     2641 tcacgtagcg cccgccgagc cggtcgaccg cccggccgaa cgcggccagc agctcagggg
     2701 tcttggtccc cttctcgtcg gcgaggatca ccgacttgcc gccgcccagc ggaagcccgg
     2761 ccatcgcgtt cttgtagctc atcccgcgcg acagccggag cgcgtcggtg accgcgtcgg
     2821 aatcctcggc atagtgccag aatcggcagc cgccggccga cgggccgagg tgcgtcgagt
     2881 ggatggcgat gatcgcctgg aggccggtcg cggaatcgtg ggcgaaatgc agcacctcgt
     2941 gggcgtcgaa atccgggagg cgccagggac aggtcatggg agcggaaaat ccattgttca
     3001 gaagagcagc cgatggggcg atcgacggga cttgaacccg cgaccctcgg taccacaaac
     3061 cgatgctcta accagctgag ctacgatcgc cacggcgcgc cccgggagcg gttccccgag
     3121 gcgccggcgt gccttaaggt tgcggccccg tgaaggcaag ctaagggccc cggatggacc
     3181 cgccagcatg aacacaggcc caatcaacgc gacgatccgc aagttcggcc atcccgccag
     3241 cctggtgcgc gagttcgagc attggctggt tctggtccgg ccagcgcagg cgacgctcgg
     3301 ctcgctggtg ctcgccgcca aaagcgacgc cgccgccttt cgcgagctgc cgcgggcggc
     3361 gttcgccgag caggccgatg ccgtcgccgc gatcgagacc ggcttggccg ctttctgccg
     3421 cttcgagcgg atcaattatc tgatgctgat gatggtcgac cgccatgtcc atttccatgt
     3481 cgttccccgc tattcgggca gccgcgaatg gaatggcctg gacttcgccg accggggctg
     3541 gccggggccg cccgatctca attcggctat ccacctttcc gccagtcagg tcagggattt
     3601 aagagacgac cttgccaaac ttttctaatt cgacatcgta tcgcccgcga aaccttgcgc
     3661 cacaccagcc agtggcgcat catcgccggg ggagaaatga catgcaggcg agtcgacgct
     3721 cggcggcggc gccagctgtg tcgctcggcg acacgcttac cgcggcggga ctcgaccggc
     3781 gtctcgcgac gctcttcgag gagatgccgc acaagatcgt cgaggtcgac ccgcggcggc
     3841 cgttccgcga tccgggggcc gtccgcgacg agttcatctt cgtgcgctcg ggaatcctgt
     3901 gcaagtacaa gaccgacggc ggcggccgcc ggcagatcgt cgcgctgcgc ttcccgggcg
     3961 aggcgatcct gccgcgcgaa gggcccgccg gctacgggct gcagggcatc gtcaagagcg
     4021 aggtgatggt cggccaggca agcgacttcg acgccatcgt cgattccaac cccgagctgc
     4081 ggcgcttctt ctggcgcctg atccagcgca acgaggcgat cggctacgag tggctggtca
     4141 attgcggccg tcgcgattcc accgcccggg ttgcccattt gctgtgcgag accgcggccc
     4201 gcagcgggat cagcggcgag catggccgga tgctcaatcc gttcacccag cagcagatcg
     4261 ccgacatcac cggccagacg tcggtcaacg tcaaccgggt gctcgccgac ctccagcgcc
     4321 aggggctgat cgagcgcagc ggccgcgaga tcgctttccc cgactgggcc gagctgcggc
     4381 gaatcgccag cttccagccg gcctatctgg aataaagggc aacggaccgc caccgtccgc
     4441 aggccgcggt cggccctcgc gcgagcatgg tgggcgcggt agggattgaa cctacgaccc
     4501 cacccgtgtg aagggtgtgc tctaccactg agctacgcgc ccgccgcgtc gaaggaaggc
     4561 cgcatatagg ggccttcccc ggcctgcatc atccgaggcg ggcctgcccg gccgcgatcg
     4621 cccaaccagg cctttggcct agtggtgggc gcggcaggga ttgaacctgc gaccccaccc
     4681 gtgtgaaggg tgtgctctac cactgagcta cgcgcccgct cggcaaggcc ccgttcgacg
     4741 ggcggcctgc ggcaggggcg cgcctttgcc ac
//
LOCUS       DASTZO010000289         3136 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_776978,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000289 DASTZO010000000
VERSION     DASTZO010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3136
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776978"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VFU20_08150"
     CDS             <1..816
                     /locus_tag="VFU20_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5286459.1"
                     /translation="TLRWGFDFFRPLKSARPSQATIDQLRQQMRRRMGDRPAGASRAP
                     DARDGPPVDAVIVREGPPPEGRGERGGFGEPGGGGPGGGFGGGRFFGGGGRQGGRLQF
                     SLTHTVNLVDEVVIRPGVPELDYLHGDAFGSSGGRPRHEVEAQVGWSNNGLGARLSAD
                     WRSATRVDSAQGGSLRFAPLATFDLRLFANLGERLDLVAKRPWLRGTQVRFEVRNLFD
                     SKPRVRDAAGEVPLSHQPDLLDPLGRTVGISVRKLFLPPPSFFRRQRPPGPAS"
     gene            857..2095
                     /gene="hisS"
                     /locus_tag="VFU20_08155"
     CDS             857..2095
                     /gene="hisS"
                     /locus_tag="VFU20_08155"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU5286460.1"
                     /translation="MATLQPVRGTQSLLGEDADRLAAVVAAFDRVRRLFGFQRVEVPV
                     IESTDVFARSIGETTDIVSKEMYTFLDRGGDSITLRPEFTAGIARAYISEGWQQHAPL
                     KLATHGPAFRYERPQKGRFRQFHQLDAEVVGAAEAAADVEILCLGDQLLRELGIADGV
                     KLELNTLGDPQTRDAWRAALVEHFGAHRADLSDDSVARLERNPLRILDSKDSKDRAIA
                     ATAPPIDDHLTPEAADFFAQVTAGLDAAKVAWTRNPRLVRGLDYYRHTAFEFVTDRLG
                     AQGTLIAGGRYDGLIEALGGPPTPAVGWAAGIERLAMMIDAPPREAPEVVLVPIGERA
                     EAEAQRILAELRQAGIAADMSYRGNMKKRLGKASDSGAAHALIIGDDELARGEAQLKN
                     LGSGEQHSVPLRGLAEALKS"
     gene            2092..>3136
                     /gene="prfA"
                     /locus_tag="VFU20_08160"
     CDS             2092..>3136
                     /gene="prfA"
                     /locus_tag="VFU20_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU5286461.1"
                     /translation="MTSISPERIASIEARKHELAEAMNAPDLPRDDLVRLSKDYAAIE
                     PVALAAREVRRLRAELEVLTALAADPTAEAELREMAEHEAADIRIRLPQAERALALKL
                     LPRDAADERAAMLEVRAGTGGDEAALFAGDLLRMYQRFAEEQGWRFELISASPSDVGG
                     YKEAIASISGTGVFAKLKFESGVHRVQRVPATESGGRIHTSAATVAVLPEAEDVDIHI
                     DDKDLRIDVYRSSGPGGQSVNTTDSAVRITHLPTGLVVIQQDEKSQHKNKAKALKVLR
                     TRLFELERERLASERAGARKSMVGSGDRSERIRTYNFPQGRVTDHRINLTLHRLPEIL
                     EGPGLEEVIAALIA"
BASE COUNT          481 a         1129 c         1095 g          431 t
ORIGIN      
        1 accctgcgct ggggcttcga tttctttagg ccgctcaagt cggcccgtcc gtcgcaagcg
       61 accatcgacc agctccgcca gcaaatgcgc cggcggatgg gtgaccggcc cgccggcgct
      121 tcccgggcgc ccgacgcgcg cgatgggccg ccggtggatg cggtgatcgt tcgggaaggg
      181 ccgccgcccg aaggccgcgg cgaacgcggc ggcttcggcg agcccggcgg gggcggcccc
      241 ggcggcggct tcggcggcgg ccggttcttc ggcggcggcg gccggcaggg cgggcggctg
      301 caattctcgc tgacccacac ggtcaacctg gtcgacgagg tcgtcatccg ccccggcgtc
      361 cccgagctcg attacctgca cggcgacgcg ttcggctcgt cgggcggccg cccgcgccac
      421 gaggtcgagg cccaggttgg ctggtcgaac aacggcctcg gggcgcggct gtcggccgat
      481 tggcgaagcg ccactcgggt cgacagcgcg caaggcggaa gcctgcgctt tgcgccgctg
      541 gcgaccttcg acctgaggct gttcgccaat ctcggcgagc gcctcgacct ggtcgccaaa
      601 cgcccgtggc tgcgcggtac gcaggtgcgc ttcgaagtgc gcaacctgtt cgattccaag
      661 ccccgggtgc gcgacgcggc gggcgaggtg ccactgagcc accagcccga cctgctcgac
      721 ccgctcgggc gcaccgtcgg catctcggtc cgcaagctgt tcttgccgcc gccgagcttc
      781 ttccgccgcc agcgcccgcc cggcccggca agctaagcct ttccgcccta cgcggaaatg
      841 tgctttagcg ccgccgatgg ccacgcttca gccggtccgg gggactcaaa gcctgttggg
      901 tgaagacgcc gaccgcctcg cggcggtcgt cgcggcgttc gaccgcgtcc gccgcctgtt
      961 cggcttccag cgcgtcgaag tcccggtgat cgaatcgacc gacgtcttcg cccgctcgat
     1021 cggcgagacc accgacatcg tctccaagga aatgtacacg tttctggacc gcggcgggga
     1081 ttcgatcacg ctccggcccg agttcaccgc cgggatcgcc cgcgcctaca tctccgaagg
     1141 ctggcagcag cacgcgccgc tgaagctcgc gacgcacggg cccgcgtttc gctacgagcg
     1201 gccgcaaaag gggcgtttcc gccagttcca ccagctcgac gccgaagtcg tcggcgccgc
     1261 cgaagccgcc gccgacgtcg agatcctgtg cctcggcgac caattgcttc gcgagctcgg
     1321 gatcgccgac ggcgtgaagc tcgaactcaa cacgctcggc gacccgcaga cccgcgacgc
     1381 gtggcgcgcc gcgctggtcg agcatttcgg agcgcaccgc gcggacctct cggatgacag
     1441 cgtcgcgcga ctggagcgca acccgttgcg gatcctcgac agcaaggatt cgaaggatcg
     1501 cgcgatcgct gcaacggcgc cgccgatcga cgatcatctg actccggaag ccgccgattt
     1561 cttcgcccag gtcaccgccg ggctcgacgc cgcgaaggtc gcctggaccc gcaacccgcg
     1621 gctggtgcgc ggcctcgatt attaccgcca caccgcattc gagttcgtca ccgaccgact
     1681 cggagcgcag ggcacgctga tcgccggcgg ccgctacgac gggctgatcg aggcgctcgg
     1741 cggccccccg actcccgcgg tcggctgggc ggccgggatc gagcggctgg cgatgatgat
     1801 cgacgcaccg ccgcgcgaag cgccggaggt cgtcctggtg ccgatcggcg agcgcgccga
     1861 agccgaagcg cagcgcatcc tcgccgagct ccgccaagcg ggcatcgccg ccgacatgag
     1921 ctatcgcggc aacatgaaaa agcggctcgg caaggcgagc gattcgggag cggcgcacgc
     1981 gctgatcatc ggcgacgacg agctcgcgcg cggcgaggcg cagctcaaga atctcggcag
     2041 cggcgagcag cactccgtgc ccctccgggg attggccgag gccctcaagt cgtgacctcg
     2101 atctcgccgg agcggatcgc ctcgatcgag gcgaggaagc atgagcttgc cgaggcgatg
     2161 aacgcgcccg acctgccgcg cgacgatctc gtgcggctgt cgaaggatta tgcggcgatc
     2221 gagccggtgg cgctcgcggc ccgcgaagtg cgccgcctgc gcgccgagct cgaggtgctg
     2281 accgcgctcg ccgccgaccc gacggccgag gccgagctgc gcgaaatggc cgagcacgag
     2341 gccgccgaca tccgcattcg cctgccgcag gccgagcgcg cgctggctct gaagctgctg
     2401 ccgcgcgacg ccgccgacga gcgcgcggcg atgctcgagg tgcgcgccgg caccggcggg
     2461 gacgaggcgg ctttgttcgc cggcgacctg ctgcgcatgt accagcgctt cgccgaggag
     2521 cagggctggc gcttcgagct catctccgcg agcccgtccg acgtcggcgg ctacaaggaa
     2581 gcgatcgcct cgatcagcgg caccggcgtg ttcgccaagc tgaagttcga aagcggcgtg
     2641 catcgcgtcc agcgcgttcc ggcgaccgaa agcggcggcc ggatccacac gtccgcggcg
     2701 accgtcgcgg tgcttcccga ggccgaggac gtcgacatcc acatcgacga caaggatctg
     2761 cgcatcgacg tctaccgctc gtcgggcccg ggcggccaat cggtcaacac caccgacagc
     2821 gcggtccgga tcacccatct cccgaccggg ctcgtggtca tccagcagga cgagaaatcg
     2881 cagcacaaga acaaggccaa agcactgaag gtgctgcgca cccggctgtt cgagctggag
     2941 cgcgagcggc tggcgagcga gcgcgccgga gcgcgcaagt cgatggtcgg ctcgggcgac
     3001 cgctcggagc gcatccgcac ctacaatttc ccgcaggggc gggtgaccga tcaccgcatc
     3061 aatctgacgc tgcaccggct gcccgagatc ctggaggggc cggggctgga ggaagtgatc
     3121 gccgcgctga tcgccg
//
LOCUS       DASTZO010000290         2396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_231921,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000290 DASTZO010000000
VERSION     DASTZO010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2396
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231921"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFU20_08165"
     CDS             complement(<1..533)
                     /locus_tag="VFU20_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286462.1"
                     /translation="MRARAGPADLWIALGGGTATLFAAFLAVDSGAQVGVGLAAGVAL
                     FIAAVAAFVVYPHVSIAATILLFALVPMLKVLVLPEIGPIKDAVVLAAVAAAIIVFVF
                     ERPHVDRWLLIPVVLLLGFYVINVGSGHGIAWAQGVRLIGEPLLLLLVGLILPEPRRS
                     FRWALGALIVTACLVAG"
     gene            complement(537..2276)
                     /locus_tag="VFU20_08170"
     CDS             complement(537..2276)
                     /locus_tag="VFU20_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286463.1"
                     /translation="MTVSDARGRPYDVPPCNSAFAAGERRAPPDSRNRLSPPQTGQDD
                     RRRREIGAGHQFDPPAPRKPTRDRPDRMTTGGVDALKAAIRRSLPLLIGLIVLGIIAV
                     NVFQQARGARYEATAKVLVTSTPLASIISGTQPGFVDPERVQQTAIGIAESSEVYRVA
                     AARNHHQHGSAGDLQSAVSVSADTNNDLISFTASSSDPEEAVGMANAVARGYIGFRKR
                     LAGSQIADTIKGLKESLDQLPSDDPKRSRVENDLSRLQVLQDNASDSQLVESATSAGK
                     TSPAPLKDSVVGLSIGLIIGLLLIAIREAVDTTARSEGDVEDLLGLPVLASVRTVPRH
                     SRLVTYGRHEAEFADAYALLAAQLVHTKAGEEGAALAITSAVPKEGKTITAANLAVAV
                     ARRGSNAILADFDFRKPGIGETFGLPDDAPGAIQVMAQEVPLESALWSASLEGTRPVL
                     ESGGSAPAHPPRTGGIRAPQSNGSLRVLPAGGATRSAPHQEQLQYLLTMLQSEADLVI
                     LDTPPALLTVEVAELSRLVDAVLLIVRQGVVTQRNLRALQRQLRTWPSEILGTVMTDV
                     RTDTKYADYGKAY"
BASE COUNT          368 a          808 c          873 g          347 t
ORIGIN      
        1 tagccggcca ccaggcaggc ggtgacgatc agcgctccga gcgcccaccg gaagctccgc
       61 cgcggctccg gcaggatcag gccgaccagc aggagcagca gcggctcacc gatcagccgg
      121 acgccctgcg cccaggcgat gccgtggccg ctgccgacgt tgatcacgta gaagccgagc
      181 aggaggacga ccgggatcag cagccagcgg tcgacgtggg gtcgctcgaa gacgaagacg
      241 atgatcgccg cggcgaccgc ggcgagcacg acggcgtcct tgatcggccc gatctcggga
      301 agaaccagga ccttgagcat cgggacgagc gcgaacagca ggatcgtcgc cgcgatcgag
      361 acatgggggt agacgacgaa ggcggcaact gccgcgatga acagggcgac gcccgcggcc
      421 agtccgactc cgacctgggc gccggagtcg acggccagga atgcggcgaa gagggtcgcc
      481 gtcccgccgc cgagggcgat ccagaggtcc gcggggccag cccgggccct cactgtctaa
      541 taggccttgc cgtagtcggc gtacttggtg tccgtgcgca catcggtcat caccgtgccg
      601 aggatctcag atggccaggt tcgcagctgg cgctgcagcg cgcgcaggtt gcgctgcgtg
      661 accacgccct gccggacgat caacagcacc gcatcgacca gcctcgagag ctcggcgacc
      721 tcgaccgtca gcagggcggg cggggtgtcg agaatgacga ggtcggcctc ggactgcagc
      781 atcgtcagca ggtactggag ctgctcctgg tgtggggcgc tgcgggtcgc accgcccgcg
      841 gggagcaccc gcagcgagcc gttcgactgc ggcgcccgga tcccgccggt ccgcggcgga
      901 tgcgcgggcg cgctgccgcc gctctcgagg accggacgcg ttccctccag cgatgccgac
      961 cagagcgccg attcgagcgg cacctcctgg gccatgacct ggatcgcccc cggagcgtcg
     1021 tcggggagcc cgaacgtctc gccgatgccg ggcttgcgga agtcgaagtc ggcgaggatc
     1081 gcgttcgatc cgcgccgggc cacggcgacc gccaggttgg cggcggtgat cgtcttgccc
     1141 tccttgggca ccgcgctggt gatcgcgagg gccgcaccct cctcgcccgc cttcgtatgg
     1201 accagctgcg cggcgagcag cgcataggcg tcggcgaact cggcctcgtg gcgaccgtag
     1261 gtgacgaggc gggaatgacg cgggaccgtc cgcacactcg ccagtaccgg cagcccgagc
     1321 aggtcctcga cgtcgccctc ggagcgggcc gtcgtatcga ccgcctcgcg gatcgcgatc
     1381 agcagcaggc cgatgatcaa cccgatcgac agcccgacga ccgagtcctt gagcggcgcc
     1441 gggctggtct tgcctgcgga cgtcgcgctc tcgacgagct ggctgtcact cgcattgtcc
     1501 tggaggacct ggagacggct caggtcgttc tcgacgcgcg agcgcttcgg gtcgtcggag
     1561 ggaagctgat ccaggctttc cttcaggccc ttgatcgtgt ccgcgatctg ggagccggcg
     1621 agtcgcttgc ggaagccgat gtagccccgc gcgaccgcgt tcgccatccc gaccgcctcc
     1681 tcggggtcgg agctggaggc ggtgaacgag atcaggtcgt tgttggtgtc ggcggagacg
     1741 ctgaccgcag actgcagatc gccggcggag ccgtgctggt ggtggttcct cgccgccgcg
     1801 acccggtaga cctcgctcga ctcggcgatg ccgatcgccg tctgctggac ccgctccgga
     1861 tcgacgaagc ccggctgggt cccggagatg atgctcgcga gcggggtact cgtgacgagg
     1921 accttggcgg tcgcttcgta tctcgcgccg cgggcctgct ggaagacgtt gacggcgatg
     1981 atcccgagca cgatcaggcc gatcagcaac ggcagcgacc ggcggatcgc agccttgagg
     2041 gcgtcgacgc ctcccgtggt catccggtcg gggcggtcgc gcgtgggttt gcggggcgct
     2101 ggcgggtcaa attggtgtcc ggcgccgatc tcgcgccgcc tacggtcatc ctgtccggtc
     2161 tggggcggcg aaagtctatt gcgtgaatcc gggggcgccc gccgctcgcc ggcggcgaag
     2221 gctgaattgc agggcggcac gtcatacgga cgtccacggg cgtcgctgac ggtcaacggc
     2281 gaacgggcgc tgtcgatacg cgggtgaagc catcgcgccg acgcgcacct gagcccctct
     2341 caccggttcg agagcggccg gcccacgaag aggacccaga tgttgccagc cgccca
//
LOCUS       DASTZO010000291         7475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_964168,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000291 DASTZO010000000
VERSION     DASTZO010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7475
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964168"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VFU20_08175"
     CDS             complement(<1..306)
                     /locus_tag="VFU20_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286464.1"
                     /translation="MNKPFDERRAAADSRLRLRCLGRFQLDDSGGAEITPRTRKARAL
                     LAFLALSRRPASRDQLADLLWSGRGAEQAKSSLRQAIFELRHLGDEQASLISLGRDEV
                     "
     gene            complement(514..1176)
                     /locus_tag="VFU20_08180"
     CDS             complement(514..1176)
                     /locus_tag="VFU20_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6766 family protein"
                     /protein_id="HEU5286465.1"
                     /translation="MAKFFKDNGLTITLMLLFLLSIVGQWLAGMHFENEQLIEHGKEP
                     IGALQFLSDPQFLSTVFENWESEFLQMSAYVVLTAFLFQRGSAESEDPDEPSRDGRLE
                     NARFAPNAPAPVRKGPAARWLYAHSLGIALLVLFAISFVLHWLFSARLAADEAALHGG
                     TADTFGQYLASPQLWFESFQNWQSEFLSTAVLVVLSIWLRQKDSPESKQITASNRKTG
                     TD"
     gene            complement(1258..1689)
                     /locus_tag="VFU20_08185"
     CDS             complement(1258..1689)
                     /locus_tag="VFU20_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HEU5286466.1"
                     /translation="MDVRPEDRQPFPGLGTDPQSVRRRVEALERLMERMFVIPGINRP
                     VGLDVILDLVPVAGSTAGAVLGAYMAWEARNLGMSKWQVARMAGNVGVDWLLGLIPWV
                     GAIPDLLFRSNTRNLRIIKRHLDKHHAGTATVDAQALPPGR"
     gene            complement(1690..3144)
                     /locus_tag="VFU20_08190"
     CDS             complement(1690..3144)
                     /locus_tag="VFU20_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5286467.1"
                     /translation="MALLVAVAAVTPACERQPQGAVKVVVIGDTPKVVDPAAGPLSPG
                     EAVLVGNVAQGLVRFDARGQIEPGLAERWNVSNDGLSYIFRLASSEWPNGRKITAHQV
                     ARILRRTIGRSSNNPLKDALGAVDEIVAMTDRVLEIRLTAPRPNLLQLLAQPEFALVR
                     EGEGSGPFRIAAGVGPAGVVRLERQVAAPDGEEGRTEELWLGGSNAALAVRGFVAGDS
                     DLVLGGTFADLPFTRRVRLPRGALRFDPVSGLFGLAPARSGGPLADPELRRLLSQAID
                     RDALIAALDVPGLVGRATLLEAALDGVPDPADPQWTTVPIGERRPQLIAAVDRLFGAE
                     DRPVIRIALPEGPGAQLLFNRLAADWAMLGIRLERAAKGQAADLRLVDAVAPSTSPAW
                     FVRQFRCAVAAICDDAADPLLEAARSAPVAAQRGALLAEAARLMDEAQLFIPIAAPVR
                     WSLVSDRIEGFAGNRFARHTLTSLEEKLNREGAR"
     gene            3283..5289
                     /locus_tag="VFU20_08195"
     CDS             3283..5289
                     /locus_tag="VFU20_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010184409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU5286468.1"
                     /translation="MADRDGVWSRGGVHVEELPDPFAPIAMPGPAGPGGEPAEAPARP
                     RRSRRRIIFYTICAVLLLALAWLIVTAPLSRALEPLDDPALLLLSEEGRPIARRGAIK
                     EAPVEAAELDPLTRAAFVAIEDRRFYRHWGVDPRGIGRAMIANARAGGVRQGGSTITQ
                     QLAKTSFLSSDRSLKRKAQEVIISFWLEAWLTKEEILSRYLSSIYFGDGVYGLRAAAR
                     HYFDRSPERLTLAQSAMLAGLVQAPSRLAPTRNLKLAQKRSRLVLAAMADIGAISEAR
                     ARAAVPARPVAQRSKVPTGTYFADWVAPAAQKAFEADFGEVKVRTTLDADLQRLAVRA
                     INRAAIGDAQAAIVAMRPDGRVVAMVGGRSYKASPFNRATQARRQPGSAFKLFVYLAA
                     LRAGWTPESIIGDRPITIGGWSPANSDGVYRGSITLREAFARSSNAATVRLSESVGRA
                     NVLRAARELGISSPLPNSPSVALGTAGVSLIELTSAYAAIASSRYPVEPRGLALAEED
                     EGFASFFRRGGTLDRRRDWAPMLDLLWEAANKGTGRRAALAVPTFGKTGTTQGNRDAL
                     FVGFAGDLVVGVWVGRDDNKSLGKITGGTAPARIWRSFMVPALAIDRRPGPPLPREFR
                     APARPQPPQRREKKSPLPEDWSDRTRGLRELVREIEDMIEDGGR"
     gene            5442..5600
                     /locus_tag="VFU20_08200"
     CDS             5442..5600
                     /locus_tag="VFU20_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEU5286469.1"
                     /translation="MAKFLKLIRNEKGATAIEYGLIAALIAVAAIGAMSSIGSALNTT
                     FGEVATNL"
     gene            complement(5621..5806)
                     /locus_tag="VFU20_08205"
     CDS             complement(5621..5806)
                     /locus_tag="VFU20_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286470.1"
                     /translation="MTGKPGPGSDAPDVVDEASIESFPASDPPAWTGGREDKPGKLKR
                     RAPGAKRKAAASSDTAA"
     gene            5911..7464
                     /locus_tag="VFU20_08210"
     CDS             5911..7464
                     /locus_tag="VFU20_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5286471.1"
                     /translation="MQARVATRVSIVGERAAWFWAGCAAISAGVLLHLPMLAEAHRMG
                     NRLAGMPIDSSMIGGMALIMGGVPLAVFGALPRRRAPHGDHAGTSYEAPDSTPLNRWH
                     AGVLLVLILGLVIDVMKPATLGFVLPGMSAEYGISKSTVAWFPFTALVGTTVGSFVWG
                     WLADVYGRRVSILLSTILFVATSVCGAMPAFHWNLVMCFLMGSSAGGMLPVVYTLLAE
                     VMPPKHRSWVLVLVGGTGLVGGYLAASSAAHAFEPVFGWRVLWLQGFPTGIALLALAH
                     LIPESPRFLAEQGRREELRRMEARFGILPKARVAAPPDAPIAAQRHGRLTAALAIAAL
                     SWSLVNFGLLLWLPTDLQARGYDSALASGILTSSALLALPTILVGAWCYARISSKWTL
                     IGTILLTVAGLLGALLPADLLTWPPLLIAVIAMLIVGTNGMIAVLLPYTAENYALGIR
                     GRATGLVAGSSKFGGVAVQGFALAGLIPTLSGAAAALLGPMALSAGLIAWAGRETRGR
                     SLRELEAGT"
BASE COUNT         1089 a         2553 c         2660 g         1173 t
ORIGIN      
        1 cacctcgtcg cggccaaggg agatcagcga tgcttgctcg tcgccgaggt gccggagctc
       61 gaaaattgcc tgcctgaggc tcgacttggc ctgctccgcg ccacgcccgc tccacagcag
      121 gtccgccagc tggtcacgag atgccggacg cctcgacaat gccaggaacg cgagcaaggc
      181 gcgcgccttg cgggtccggg gcgtaatctc ggcgccgcct gaatcgtcca gctggaagcg
      241 cccgaggcat cgaagccgca ggcggctgtc ggcggcggcg cgccgttcgt cgaatggctt
      301 gttcacggcc tgccgaagcc gaatttcgcc gcggttcgcg gccgcgcggc aagatccgcg
      361 gttagggaca aaggccggtg tggcaatccg atcttccccc tttcgctcgc cgggccgcgc
      421 ctaccgccag tatcggcgtt tcgttgcgca gcatcaaccg catgggttgc tgtcctcgcc
      481 ggccgtggcg gccagcatca gcggaccggg gcttcagtcg gttcccgttt tgcggttgga
      541 tgcggtgatt tgcttggatt cgggcgaatc cttctgccga agccagatgc tgaggacgac
      601 cagcaccgcg gtcgacagga attcgctctg ccagttctgg aacgattcga accacaattg
      661 cgggctggcg agatattggc cgaacgtgtc cgccgtgccg ccgtgcaggg cggcttcgtc
      721 ggccgccagc cgggcgctga acagccagtg caggacgaac gagatcgcga acagcaccag
      781 cagcgcgatt cccagcgagt gggcataaag ccagcgcgcg gcgggcccct tgcggacggg
      841 ggccggcgcg ttgggcgcga agcgggcgtt ctcgagccgg ccgtcgcgcg acggctcgtc
      901 ggggtcctcg gattcggccg atccgcgctg gaacaggaag gcggtgagga cgacataggc
      961 cgacatctgc aggaactcgc tttcccaatt ctcgaacacg gtcgagagga attgcgggtc
     1021 cgagaggaat tgcagggcgc cgatcggctc cttgccatgc tcgatcagct gctcattctc
     1081 gaaatgcatc ccggcgagcc actggccgac gatgctcaag aggaacagca gcatcagagt
     1141 gatggtaagg ccgttgtctt tgaagaactt agccatggga cctcctgttc ccatgcgcaa
     1201 ttcgccgctc gggcttgcgg ttcccgcact taaccctggt gaattgttgg ccggcgctca
     1261 gcgcccgggc ggcagggctt gcgcgtcgac ggtcgcggtg cccgcgtggt gcttgtcgag
     1321 gtggcgcttg atgatgcgca aattgcgcgt gttcgagcgg aacaacaggt cggggatcgc
     1381 gccgacccac gggatcaggc cgagcagcca gtcgacgccg acgttgccgg ccattcgtgc
     1441 gacctgccat ttggacatgc cgaggttgcg cgcttcccac gccatatagg cgccgagcac
     1501 ggccccggcc gtgcttccgg cgaccggcac caggtcgagg atcacgtcga gcccgaccgg
     1561 gcggttgatc ccggggatga cgaacatccg ctccatcagc cgctcgagcg cctcgacccg
     1621 gcggcggacc gactgcgggt cggtgccgag gccgggaaac ggctgccggt cttcgggtcg
     1681 aacgtccatt taccgggcgc cttcgcggtt gagcttctct tcgaggctgg tcagcgtgtg
     1741 gcgggcgaag cggttcccgg cgaagccctc gatgcggtcg gagaccagcg accagcggac
     1801 cggcgccgcg atcgggatga acagctgcgc ctcgtccatc aggcgcgcgg cttcggcgag
     1861 caaggcgccg cgctgcgccg cgaccggcgc cgagcgggcg gcctcgagca gcgggtcggc
     1921 cgcgtcgtcg cagatcgccg cgaccgcgca gcggaactga cggacgaacc aggccggcga
     1981 ggtcgacgga gcgaccgcgt cgaccagcct gaggtcggcc gcctggccct tggccgcacg
     2041 ctcgagcctg atcccgagca tggcccagtc ggcggcgagc cggttgaaca gcagttgcgc
     2101 gcccgggccc tcgggcagcg cgatccggat caccggacgg tcctcggcgc cgaacaggcg
     2161 gtcgacggcg gcgatcagct gcggccggcg ctcgccgatc gggaccgtcg tccactgcgg
     2221 atcggccggg tccgggactc cgtcgagcgc agcctcgagc agggtcgccc ggccgaccag
     2281 ccccggaacg tcgagcgccg cgatcagcgc gtcccggtcg atcgcctgcg acagcagccg
     2341 ccggagctcg ggatcggcga gcgggccgcc gcttcgcgcc ggggcgagcc cgaacagtcc
     2401 cgacaccggg tcgaagcgca gcgctccgcg cggcagccgg actcggcggg tgaagggaag
     2461 gtcggcgaac gtgccgccga gcaccaggtc ggagtccccg gcgacgaaac cgcggaccgc
     2521 aagggcggcg ttcgagccgc ccagccaaag ctcctcggtg cggccctcct ccccgtcggg
     2581 agcggcgact tgccgctcga gccggacgac gccggccggc ccgaccccgg cggcgatccg
     2641 gaacgggccg ctgccttcgc cttcgcggac cagagcgaac tcgggctggg cgagcaattg
     2701 cagcagattg gggcgcgggg ccgtcagccg gatctcgagc acccggtcgg tcatggcgac
     2761 gatctcgtcg accgcgccga gcgcgtcctt gagcgggttg ttgctgctgc gcccgatcgt
     2821 ccggcggagg atccgggcga cctggtgagc ggtgatcttg cgcccgttcg gccattcgct
     2881 ggatgcgagc cggaagatgt agctgagccc gtcgttgctg acgttccagc gctcggcgag
     2941 ccccggctcg atctggccgc gggcgtcgaa ccgcaccagc ccctgcgcga cattgccgac
     3001 caggaccgct tcgcccgggc tcagcggccc ggcggccgga tcgaccacct tgggggtgtc
     3061 gccgatgacg acgaccttga cggcgccttg cggctggcgc tcgcaggccg gagtgacggc
     3121 ggcgacggcg acgagcagcg ccattgccag ggcgcgccgg agagcggacg gcggcatggc
     3181 aggcgtgtag cctcaatcgc aggttcagca aatgtctgga tatatgcccg gcactgtttt
     3241 actggactaa agcagttgcg agttccgcaa gaaggatcgt gggtggcgga cagggacgga
     3301 gtctggagcc ggggcggagt ccatgtcgag gagcttcccg acccgttcgc gccgattgcg
     3361 atgcccggcc cggccgggcc gggcggcgag ccggccgagg ccccggccag gccgcgacga
     3421 agccggcggc ggatcatttt ctacaccatc tgcgccgtcc tgctgctggc actggcgtgg
     3481 ctgatcgtca ccgcgccgct gtcgcgggcg ctggagccgc tcgacgaccc ggcgctgctg
     3541 ctgttgtcgg aagagggccg gccgatcgcc cggcgcggag cgatcaagga agcgccggtc
     3601 gaggcggccg agctcgaccc gctgacccgc gccgcgttcg tcgccatcga ggaccggcgc
     3661 ttctaccggc actggggcgt cgatccgcgc ggcatcggcc gggcgatgat tgccaatgcc
     3721 cgggccggcg gagtccgcca gggcggcagc acgatcaccc agcagctcgc caagaccagc
     3781 ttcctgtcga gcgaccggag cctcaagcgc aaggcgcagg aagtgatcat ctccttctgg
     3841 ctcgaagcct ggctgaccaa ggaggaaatc ctctcgcgct atctgtcgag catctatttc
     3901 ggcgacggcg tctatggcct gcgcgccgcc gcccggcatt atttcgaccg ctcgccggag
     3961 cggttgacgc tggcgcagtc ggcgatgctc gccggtctcg tccaggcgcc gtcgcggctc
     4021 gctccgaccc gcaacctgaa gcttgcccag aagcgcagcc ggctggtgct cgcagcgatg
     4081 gccgacatcg gcgcgatcag cgaggcgcgt gcccgagcgg cggttcccgc gcgcccggtc
     4141 gcgcagcgct cgaaagtccc gaccgggacc tatttcgcgg actgggtggc gccagccgcg
     4201 caaaaggcgt tcgaggccga tttcggcgag gtcaaggtgc gcaccaccct cgatgccgac
     4261 ctgcagcggc ttgcggtgcg ggcgatcaac cgcgccgcga tcggcgatgc acaggcggcg
     4321 atcgtcgcga tgcgtcccga cgggcgggtc gtggcgatgg tcggcgggcg cagctacaag
     4381 gcgagcccgt tcaaccgcgc gacccaggcg cggcgccagc ccggatcggc gttcaagctg
     4441 ttcgtctatc tcgccgctct gcgcgccggc tggaccccag agagcatcat cggggaccgg
     4501 ccgattacca tcggcggctg gagcccggcg aacagcgacg gggtctatcg cggctcgatc
     4561 actctgcgcg aggcctttgc gcggtcgagc aacgcggcga cggtgcgatt gtcggaaagc
     4621 gtcggccggg ccaatgtcct ccgcgccgcg cgcgagctcg gcatctcgag cccgctgccc
     4681 aacagcccca gcgtcgctct cggcaccgcc ggtgtcagcc tgatcgagct gacctcggcc
     4741 tatgcggcga tcgccagcag ccgctacccg gtcgagccgc gcggcctcgc gctcgctgag
     4801 gaggatgagg gattcgccag cttcttccgc cgcggcggga ccctcgaccg gcgccgcgac
     4861 tgggcgccga tgctcgacct gctgtgggaa gccgccaaca agggcaccgg ccgccgagcc
     4921 gcgctcgccg tcccgacctt cggcaagacc gggaccacgc aaggcaaccg cgatgccctg
     4981 ttcgtcggct ttgccggcga cctggtggtc ggggtgtggg tcggccgcga cgacaacaag
     5041 tcgctgggca agatcaccgg cggcaccgcc ccggcgcgca tctggcgcag cttcatggtc
     5101 ccggcgctgg ccatcgaccg ccgcccgggc ccgccgctgc cgcgcgagtt ccgggcgccg
     5161 gcaaggccgc agccgccgca gcgtcgcgaa aagaagagcc cgcttcccga ggactggagc
     5221 gatcgcaccc gcgggttgcg cgaactggtg cgcgaaatcg aggacatgat cgaggacggc
     5281 ggccgctgat tgcggcgaac atagttaacc ggcggaaacc tgccgcttta aggctttctc
     5341 aacccctccc gggctaattc ccgatgcaac ctacgcgaac ggctgcgcag gtgggggaac
     5401 agtcggttcc cgagttaagt ggagccttaa ggagaccaga gatggccaag tttctgaaac
     5461 tgattcgtaa cgagaagggc gcgaccgcca ttgaatacgg tctgatcgcc gctctcatcg
     5521 ccgttgcggc gatcggcgcc atgtcgagca tcggcagcgc gctgaacacg accttcggtg
     5581 aagtcgcgac caacctttaa tcgcgatttt ccgatccaag tcaggcggcg gtgtcggaag
     5641 acgccgccgc ctttcttttt gcgcccggag cgcgacgctt gagcttgccc ggcttgtctt
     5701 cgcggccgcc ggtccacgcc ggcgggtcgc tggccggaaa gctttcgatc gacgcttcgt
     5761 cgaccacgtc cggcgcgtcg ctgccaggcc cgggcttgcc ggtcatcgaa gagcatgcct
     5821 cgccatcgcc ccgctcatag caggttgcga acggcttcgg ggacttgcct gaattgaact
     5881 ttggccggcg gtcggccaat tgcggtcgcg atgcaggccc gggtcgcgac gcgcgtttca
     5941 atcgtcggcg agcgcgccgc gtggttctgg gcgggctgcg cggcgatctc agccggcgtg
     6001 ctgcttcacc tgccgatgct cgccgaggcg caccgcatgg gcaaccgcct cgccggcatg
     6061 ccgatcgatt cgagcatgat cgggggcatg gcgctgatca tgggcggagt gccgcttgcc
     6121 gtcttcggag ccttgcccag gcggcgggcg ccgcacggcg accatgccgg gacgagctac
     6181 gaggcgccgg actcgacgcc gctcaatcgc tggcacgccg gcgtcctcct ggtcctcatc
     6241 ctcggcctgg tcatcgacgt gatgaagccg gcgacgctgg gcttcgtgct tccgggaatg
     6301 agcgccgaat atggaatcag caaatccacc gtcgcctggt tcccgttcac ggccctggtc
     6361 gggacgacgg tcggctcgtt cgtctgggga tggctggcgg acgtctacgg ccggcgcgtg
     6421 tcgatcctgc tgtccaccat cctgttcgtc gcgacctccg tctgcggggc gatgccagcg
     6481 ttccactgga acctggtgat gtgcttcctg atgggcagct cggcgggcgg aatgctgccg
     6541 gtggtctaca cgctgctcgc cgaggtgatg ccgcccaagc accgcagctg ggtgctggtg
     6601 ctggtcggcg ggaccggcct tgtcggcggc tatctggccg cgagctcggc cgcccacgcg
     6661 ttcgagccgg tcttcggctg gcgagtcctt tggctgcagg gattcccgac cgggatcgcg
     6721 ttgctggcgc tggcccatct gatccccgaa tcgccgcgct tcctcgccga gcaggggcgg
     6781 cgcgaagagc tccggcggat ggaagcgagg ttcggcatac tgcccaaggc gcgcgtggcg
     6841 gcacccccgg acgcgccgat cgccgcgcag cgccacgggc gcctgacggc cgcgctcgcc
     6901 atcgccgctc tgtcctggag cctggtgaat ttcggtcttc tgctgtggct gccaaccgac
     6961 ctccaggcgc gcggctacga ttcggcgctc gccagcggca tccttaccag ctcggcgctg
     7021 ctcgctctgc cgacgatttt ggtgggcgcc tggtgctatg cccggatcag cagcaagtgg
     7081 acgctaattg gcaccatcct cctgactgtc gccgggctgc tcggggcgct gctgcccgcc
     7141 gacctgctga cctggccgcc gctgctgatc gcggtcatcg ccatgctgat cgtcggcacc
     7201 aacggcatga tcgcggtcct cctcccgtac accgcggaga attatgcgct gggaatccgc
     7261 ggccgggcga ccgggctggt cgccgggagc agcaagttcg gcggcgtcgc ggtgcagggg
     7321 ttcgcgctcg ccgggctgat cccgactctg agcggggcgg ccgccgcttt gctcgggccg
     7381 atggcgctgt cggcggggct gattgcctgg gccggacgcg agacccgggg gcgcagcctc
     7441 agggagctcg aagcggggac ctagcccggt gcccg
//
LOCUS       DASTZO010000292         7025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_938397,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000292 DASTZO010000000
VERSION     DASTZO010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938397"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VFU20_08215"
     CDS             <1..188
                     /locus_tag="VFU20_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286472.1"
                     /translation="SPGVLVGRCRISQDRLVARCRIGKGAATIVADADFLNVEDLDGP
                     TDRNLEGMLAELAALER"
     gene            493..984
                     /locus_tag="VFU20_08220"
     CDS             493..984
                     /locus_tag="VFU20_08220"
                     /inference="COORDINATES: protein motif:HMM:NF014439.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEU5286473.1"
                     /translation="MALEHLFQGSALNAVDAKGRVSVPAFLRSVVERRGDARSIVLAA
                     HESFPCLAAYDPAYAALKHSKLERLLEKEETSAQAQLEYQQRNLMAFAATEEVPYDSS
                     GRIVLPTMMRRKGSIEEVALFLGAGETFQIWNPALFLADDRIPEDMKDIARYRLEERG
                     LGA"
     gene            981..1934
                     /gene="rsmH"
                     /locus_tag="VFU20_08225"
     CDS             981..1934
                     /gene="rsmH"
                     /locus_tag="VFU20_08225"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU5286474.1"
                     /translation="MTRASSGSRHLPVLADEVVQALAIARGETLVDGTFGAGGYTRAM
                     LAAGAGRVIGFDRDPDAIAEGPTLVPDPRLTLVEERFSQMDRVLAERGIGLVDGIALD
                     IGVSSMQLDRADRGFSFQADGPLDMRMSKSGLTAAEFLNQADEAEIARILKDYGEEPR
                     ARAVARAIAAARPIERTTELAAIVRRAAGFRPGQRTDPATRTFQAIRIHLNAELDELE
                     DGLAAAERALKPGGRLAVVTFHSLEDRIVKRFLRERSGASPAGSRHRPLLTDPDEPTF
                     ERVAKPVTPSEREIAANPRARSARLRSAVRTAAPAWKGTRG"
     gene            1931..2680
                     /locus_tag="VFU20_08230"
     CDS             1931..2680
                     /locus_tag="VFU20_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286475.1"
                     /translation="MSARGFRSVFMVASCAGAALGCYLVSLRVASERAALENVETKIV
                     LAQRDIRLLQTEIGTRGRLAQLERWNVKVLALSAPSADQFLDGGFALAKLVAPQDKVD
                     LDAPVVLAAAPAGGPRPQVEGRQPPQGPDAGQGVSAGEMLHVASLKREIRPVEKAVAA
                     PLPAPEKAADRPVSAAKATPKGQGPKAPAPKLQAAKIQAAKAPAATPRPASAERKSAA
                     SDPLAPLEIAAKPKTLASTTRNSGKDSGSKQ"
     gene            2776..4392
                     /locus_tag="VFU20_08235"
     CDS             2776..4392
                     /locus_tag="VFU20_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5286476.1"
                     /translation="MHQRLMFGMLVYAGVIALIVARILFLAAFGDHAGRKVGITALIP
                     ERGDIVDREGQPLARTIDAWTIGMHPNKVIGDKLEIARALERLMPERDAATYHAMLRS
                     GRNFFYLRRRAPPGLVEAVNALGEPGLSLDREPDRLYPQTSLAAHVIGYTDVDGRGTA
                     GMERAFDERLSDPATRGEPLTLSISSRLQQALEHELLDAMTEFSAIGAAGIVMDIHTG
                     EVLAMTSLPQLNPNAAGQGSEEARFNRATLGVYELGSTFKPFTVAMAMDSGIIKSFGQ
                     IYNCPDELIAYGHVIHDTHPFGRQCTVAEIMKESSNIGTAQIAAQVGSARQRAFLDKM
                     GFLSPIAVELKERGRTLSPGSNWNQIETMTVGYGHGIAVTPLHLATGYATLFNGGIYR
                     PPTLIKVDRTHPVAKGRRVFSEETSYKMRALLRLVVTHGTGRKADAPGYRVGGKTGSA
                     EKYHNRSLMVTSFAGVFPMDEPRYVIVAMLDEPKATAETYGFRTAGWNVAPVISRTVS
                     RIAPMLGVRPDKRREPNLAEVLPFVHETEH"
     gene            4397..5812
                     /locus_tag="VFU20_08240"
     CDS             4397..5812
                     /locus_tag="VFU20_08240"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU5286477.1"
                     /translation="MRLRELAGIDSDSEVTGFAIDHRKVAPGSVFGAFKGAVFDGEDF
                     IGQAVTRGAVAVVARPEARVERVPHLADVAPRRLFASLAAKFYGPYPEVAVAVTGTNG
                     KTSTVEMARQMWRMGGHRSASIGTLGVTTSDDQVTTGLTTPDIVTFLSNMAGLKRMGI
                     SHVAYEASSHGLDQHRCEGVPLAAAAFTNFSRDHLDYHPSMEAYFEAKMRLFDELLGP
                     DGTAVVWTDDARSEDVIARVRRRGSALMTVGRAGETIRLLEQTPTPLGQTVMLGHSGK
                     THRLALPLIGAYQAANVLVAAGLVMATGTGFEAAFSAMQRVSPVRGRLERAVISPRGA
                     PVYIDYAHTPDALEAAIAALRPHVEGRLIIVFGAGGDRDEGKRPEMGAVAAKMSDLVI
                     VTDDNPRTEDPAGIRAAVLAGAPGATEIAGRREAIAEAIRIARAGDIVLVAGKGHEQG
                     QIVGDRVLPFDDVVVARECAA"
     gene            5809..>7025
                     /gene="murF"
                     /locus_tag="VFU20_08245"
     CDS             5809..>7025
                     /gene="murF"
                     /locus_tag="VFU20_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU5286478.1"
                     /translation="MSAQGTTLWTSTDIAAATGGSVHGVFEVTGVTFDSREVRAGDLF
                     VAMPGTVHDGHEFVERAVAAGAAGALVSKPIESPYVLVEDTARALEDLGRASRARSKA
                     KIIAVTGSVGKTSTKEAIYAGLERWRRGAVHRSVKSYNNHTGVPLSLARMPADTEYAV
                     LELGMSNAGEIAALTRMVRPHVALITAIAPAHIENLKSEEAIADAKAEIFEGLEPGGI
                     AILPNDSPHRDRLVKAARRRTDRIITFGRGDADVHAIHAVSSNGGGSLITASLLDCDL
                     TFTISQRGDHWVSNALAALAAVEAVGGDVATAGLALADMGALKGRGERHRVPVAGGEL
                     LLIDESYNANPASMAATLKSLGAESGIERRIAVLGPMRELGAHSEALHAGLAPAVIDA
                     RVDRLILIGEELRP"
BASE COUNT         1157 a         2474 c         2427 g          967 t
ORIGIN      
        1 cctctccggg cgtgctcgtc ggccgctgcc ggatcagcca ggatcgcctg gtcgcgcgct
       61 gccggatcgg caagggcgcg gcgaccatcg tcgccgacgc cgacttcctc aacgtcgagg
      121 acctcgacgg cccgaccgac cggaacctcg aagggatgct ggccgagctg gccgcgctcg
      181 agcggtgacg gattcgcgaa accgccgagt cgccccggcg atcaaccgcg cacagactta
      241 tccacaaaga aagccgcgag aacagctgtg gaacatcgcc aagaactgcg accaaaaagt
      301 cgcggttttc cgctgctggg catgaattcc cactagaatg gcatcaaaac ccattgtatc
      361 cccaccaagc ttgagttacc aacaccgact gtagcgcggg ggatgaccca atcatttgga
      421 ccctgacgcg ggaccgcggt ttctccgcgc gcccgcgaac gaatccactt gcgctgcgaa
      481 cggggagagg acgtggcgct agagcatctg ttccagggca gcgcgctgaa cgcggtcgac
      541 gccaagggcc gcgtctccgt ccccgccttc ctgcgcagcg tcgtcgagcg ccgcggcgac
      601 gcccggagca tcgtgctggc cgcccacgag agcttcccct gcctcgccgc ctacgacccc
      661 gcctacgcgg cattgaagca ttccaagctc gagcggctgc tcgagaagga agagaccagc
      721 gcccaggccc agctcgaata tcagcaacgc aatttgatgg ccttcgccgc caccgaggaa
      781 gtgccctacg acagctccgg ccggatcgtc ttgccgacga tgatgcgccg caagggatcg
      841 atcgaagagg tcgccctgtt cctcggcgcg ggcgagacct tccagatttg gaacccggcg
      901 ctgttcctcg cggatgaccg aatccccgag gacatgaagg acatcgcccg gtaccggctc
      961 gaggaaaggg ggctcggcgc atgacgcgcg cttcctccgg ttcccgccac ttgccggtgc
     1021 tggccgatga agtcgtccag gccctcgcca ttgcccgtgg agagaccctg gtcgacggca
     1081 ctttcggggc gggcggctat acgcgcgcca tgctcgcggc gggggcggga cgagtgatcg
     1141 gcttcgaccg cgatccggat gcgatcgcgg aagggccgac actcgttccg gacccaagat
     1201 tgaccctggt cgaggagcgt ttctcgcaaa tggaccgggt gctcgccgag cgtggcatcg
     1261 gcctcgtcga cggcatcgcg ctcgatatcg gcgtgtcgtc gatgcagctg gatcgggcgg
     1321 accgcggctt ttccttccag gccgacggtc cgctcgacat gcgcatgagc aagtcggggc
     1381 tgaccgccgc ggagttcctg aaccaggccg acgaggccga gatcgcccgg atcctcaagg
     1441 actatggcga ggagccgcgg gcccgcgccg tcgcccgagc gatcgccgcc gcccgcccga
     1501 tcgagcggac caccgagctg gccgcgattg tccgccgcgc cgccggcttc cgtccgggcc
     1561 agcggaccga ccccgcgacc cgcaccttcc aggcgatccg gatccacctg aacgccgagc
     1621 tcgacgagct ggaagacggc ctcgccgccg ccgagcgcgc gctgaagccc ggcggccggc
     1681 tcgcggtggt gacgttccac agcctcgaag accggatcgt caagcgcttc ctgcgcgagc
     1741 gcagcggagc gtctcccgcc ggctcgcgcc accggccgct gctcacggac cccgacgagc
     1801 cgaccttcga gcgggtcgcc aagccggtca ccccgtcgga gcgcgagatc gccgccaacc
     1861 cgcgcgcccg ctcggcccgg ctgaggagcg cggtccgcac cgcggcgccg gcctggaagg
     1921 ggacgagagg atgagcgcga gaggcttccg ctcggtgttc atggtcgcca gctgcgccgg
     1981 cgcggcgctc ggctgctacc tggtgtcgct gcgggtcgcc tcggagcggg ccgcgctcga
     2041 gaatgtcgag acgaagatcg tgctcgccca gcgcgatatc cgcctgctcc agaccgagat
     2101 cggcacccgc ggccggctcg cccagctgga acgctggaac gtcaaggtgc tggcgctgtc
     2161 ggcgccgtcg gccgaccagt tcctcgacgg cggcttcgcg ctcgccaagc tggtcgcgcc
     2221 gcaggacaag gtcgacctcg acgcgccggt ggtgctcgcc gccgctccgg ccggcgggcc
     2281 gaggccgcag gtcgaaggcc gccagccgcc gcaggggccg gacgccgggc agggggtttc
     2341 ggccggcgag atgctccacg tcgccagcct caagcgcgaa atccgcccgg tcgaaaaggc
     2401 ggtcgcggca ccgctgccgg cgcccgagaa agcggccgac aggccggtga gcgcggccaa
     2461 ggccactccg aaaggccaag gcccgaaagc cccggccccg aaactccagg ccgcgaaaat
     2521 ccaggccgca aaagccccgg ccgcgacgcc caggccggca tccgccgagc gcaaatccgc
     2581 ggcttccgac ccgctggcgc cgctcgaaat cgcggccaag cccaagacgc ttgcttcgac
     2641 caccaggaat tccggcaagg actccggctc gaagcaatga acgcgccgac gtccgccctc
     2701 gtcgcccaac ggccagagcg gctccgccca gaacgactta ggctcgtcgg ccaacgacgc
     2761 caaatcctgg ccgtaatgca ccagcggctg atgttcggaa tgctggtgta cgccggggtg
     2821 atcgcactga ttgtcgcgcg gatcctgttc ctcgcggcgt tcggcgacca cgccgggcgc
     2881 aaggtcggga tcaccgcgct gattccggaa cgcggcgaca tcgtcgaccg cgaaggtcag
     2941 cccctggcgc ggaccatcga tgcctggacc atcggcatgc acccgaacaa ggtgatcggc
     3001 gacaagctcg agatcgcccg cgcgctcgag cggctgatgc cggagcgcga cgccgcgacc
     3061 tatcacgcga tgctgcgctc gggccgaaac ttcttctact tgcgccgccg ggcgccgccc
     3121 gggctggtcg aggcggtcaa cgccctgggc gagccgggcc tgtcgctcga ccgcgagccc
     3181 gaccggctct acccgcagac cagccttgcc gcgcacgtca tcggctacac cgacgtcgac
     3241 ggccgcggca ccgccggaat ggagcgcgcg ttcgacgagc gcctgtcgga cccggccact
     3301 cgcggcgagc cgctgaccct gtccatctcg agccgcctgc agcaggcgct cgagcacgag
     3361 ctgctcgacg cgatgaccga attttcggcg atcggcgcgg ccgggatcgt catggacatc
     3421 cacaccggcg aagtgctggc gatgacctcg ctgccgcaat tgaacccgaa cgccgccggc
     3481 cagggatcgg aggaggcgcg gttcaaccgg gcgacgctgg gggtctacga gctcggatcg
     3541 acgttcaagc cgttcaccgt cgccatggcg atggacagcg gaatcatcaa atcgttcgga
     3601 cagatctaca attgcccgga cgagctgatc gcctacgggc acgtgatcca cgacacgcat
     3661 cccttcgggc gccagtgcac cgtcgccgag atcatgaagg aaagctccaa catcggcacc
     3721 gcccagatcg ccgcgcaggt cggatcggcg cggcagcgcg cgttcctcga caagatgggc
     3781 ttcctcagcc cgatcgcggt cgagctgaag gagcgcggcc ggactctgag cccgggcagc
     3841 aactggaacc agatcgagac gatgaccgtc ggctacgggc acggcatcgc ggtcactccg
     3901 ctgcatctgg cgacgggcta cgcgaccctg ttcaacggcg gcatctatcg gccgccgacg
     3961 ctcatcaagg tcgaccgcac ccacccggtc gcgaagggcc gcagggtgtt cagcgaggaa
     4021 accagctata agatgcgggc tttgttgcgg ctggtggtca cgcatggcac cggccgcaag
     4081 gcggacgcgc ccggctaccg ggtcgggggc aagaccggaa gcgccgagaa ataccataac
     4141 cgctcgctga tggtgaccag cttcgccggc gttttcccga tggacgaacc ccgctatgtg
     4201 atcgtcgcga tgctcgacga acccaaagcg accgccgaga cctacggctt ccggaccgcc
     4261 ggctggaacg tcgcgccggt gattagccgc acggtgagcc ggatcgcgcc gatgctcgga
     4321 gtccgccccg acaagaggcg cgagcccaac ctcgccgagg tgctgccttt cgttcacgaa
     4381 acggagcact gagaccgtgc gccttcggga gctggccggc atcgatagcg attccgaggt
     4441 cacgggcttt gcgatcgatc accgcaaggt cgctcccgga agtgtcttcg gggcgttcaa
     4501 gggagcggtg ttcgacggcg aggatttcat cggccaggcg gtgacccgcg gagcggtggc
     4561 ggtggtcgcg cgccccgagg cccgggtgga acgggtcccg cacctcgccg atgtggcgcc
     4621 gcggcggctg ttcgcgagcc tcgcggcgaa attctacggg ccttatccgg aagtcgcggt
     4681 cgcggtcacc gggaccaacg gcaagacctc gaccgtcgag atggcgcggc agatgtggcg
     4741 gatgggcggc caccgctcgg cgtcgatcgg cacgctcggg gtgaccacct cggacgacca
     4801 ggtgacgacc ggcctgacga cgcccgatat cgtcaccttc ctgtcgaaca tggccggcct
     4861 caagcgcatg ggcatcagcc atgtcgccta cgaggcctcg tcccacggcc tcgaccagca
     4921 ccgctgcgag ggagtcccgc tggccgccgc ggcgttcacg aacttttccc gcgaccatct
     4981 cgactaccac ccgtcgatgg aggcctattt cgaggccaag atgcggctgt tcgacgagct
     5041 gctcgggccg gacggaacgg cggtggtgtg gaccgacgat gccaggtccg aggacgtgat
     5101 cgcccgcgtg cgccgccgcg gcagcgccct gatgaccgtc ggccgcgccg gtgaaaccat
     5161 ccggctgctt gagcagaccc cgacgccgct cggccaaacg gtgatgctcg gccattcggg
     5221 caagacccac cggctcgccc tgccgttgat cggcgcctac caggcggcca acgtgctggt
     5281 cgcggcgggt ctggtgatgg cgacggggac cggcttcgag gcggccttct cggcgatgca
     5341 gcgggtgtcg ccggttcgcg gccggctcga gcgcgcggtg atcagcccca ggggcgctcc
     5401 ggtctatatc gactatgcgc acacgcccga cgcgctcgaa gcggcgatcg ccgcgctccg
     5461 gccgcacgtc gagggaaggc tgatcatcgt gttcggcgcc ggcggcgacc gcgacgaggg
     5521 caagcggccc gagatgggcg cggtcgcggc gaagatgtcc gacctggtga tcgtcaccga
     5581 cgacaatccg cgcaccgagg acccggccgg gatccgcgcc gccgtgctcg ccggtgcgcc
     5641 aggggcgacc gagatcgccg gccgccgcga ggcgatcgcc gaggcgatcc ggattgcccg
     5701 ggcgggcgac atcgtgctgg tcgccggcaa gggccacgag cagggccaga tcgtcggcga
     5761 ccgggtgctc ccgttcgacg acgtggtcgt cgcccgcgag tgcgcggcat gagcgcccaa
     5821 ggcactacct tgtggacctc gaccgacatt gccgcggcga ccggcggcag cgtgcacggc
     5881 gtgttcgagg ttaccggagt gacgttcgac agccgcgagg tgcgggcggg cgacctgttc
     5941 gtcgccatgc cggggaccgt ccacgacggg catgagttcg tcgagcgagc ggtcgccgcg
     6001 ggagcggccg gcgcgctggt gtcgaagccg atcgagtctc cgtacgtcct ggtcgaggac
     6061 acggcgcggg cgctcgagga cctcggccgg gcgtcgcgcg cccggtcgaa ggcgaagatc
     6121 atcgcggtca ccggatcggt cggcaagacg agcaccaagg aagcgatcta cgcaggcctc
     6181 gagcgctggc ggcgcggcgc ggtccaccgc tcggtcaaga gctacaacaa ccataccggg
     6241 gtgccgctga gcctcgctcg gatgccggct gataccgaat atgcggtgct cgagctcggc
     6301 atgagcaacg ccggcgagat cgccgcgctg acccggatgg tgcggccgca cgtcgctctg
     6361 atcaccgcga tcgccccggc gcatatcgaa aacctcaagt cggaagaggc gatcgccgac
     6421 gccaaggccg agattttcga aggcctcgag cccggcggaa tcgcgatcct gcccaacgac
     6481 agcccgcacc gcgaccggct ggtcaaggcg gcgcggcgcc gcaccgaccg gatcatcacc
     6541 ttcggccgcg gcgacgccga cgtccacgcg atccacgcgg tcagctcgaa cggcggcggc
     6601 agcctgatca ccgcctcgct gctcgattgc gacttgacct tcaccatttc ccagcgcggc
     6661 gaccattggg tgtcgaacgc gctcgcggcg ctggccgcgg tcgaggcggt cggcggcgat
     6721 gtcgcgaccg ccgggctcgc gctggccgac atgggcgccc tcaagggccg cggcgagcgg
     6781 caccgagttc cggtggccgg cggcgagctg ctgctgatcg acgaaagcta caacgccaac
     6841 ccggcgtcga tggccgcgac cctcaagagc ctcggggccg aaagcggcat cgagcggcgg
     6901 atcgcggtgc tcgggccgat gcgtgagctc ggggcccaca gcgaggccct ccacgccggc
     6961 ttggcgccgg cggtgatcga cgcacgggtc gaccggctga tcctgatcgg cgaggagctg
     7021 cggcc
//
LOCUS       DASTZO010000293         3006 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_494814,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000293 DASTZO010000000
VERSION     DASTZO010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3006)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3006)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3006
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_494814"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VFU20_08250"
     CDS             <1..302
                     /locus_tag="VFU20_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010183201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HEU5286479.1"
                     /translation="AAGVQPVDGVFADFKDPKGLRSESEAARREGFTGKLAIHPDQVA
                     IINAAFTPSKAEIAHARAIVAAFEAKPGAGVLSVGGRMVDRPHLLQAQRILARAQ"
     gene            316..1374
                     /locus_tag="VFU20_08255"
     CDS             316..1374
                     /locus_tag="VFU20_08255"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010403753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HEU5286480.1"
                     /translation="MTTTARAWGVTGAESPVVPLTIERRELRPEDVAIRISHCGICHT
                     DLHFAHNDWGMSEYPMVPGHEIVGTVTGVGSGVTRYQAGDRVAVGCLVDSDLSCEQCR
                     AGYEQFCVDMPTFTYSGRDRQSGELTQGGYSDHVIVRQEFVCRVPDSFDMARAAPLLC
                     AGITTYSPLRRFKVGPGQRVAVVGLGGLGHMGVKLAAAMGAHVTMVTTSPAKGEDARQ
                     LGADEVLVSTDPEAMAAAAGRFHFVLDTIPVAHEVQPYLDLLGPHGALVLVGALEPLP
                     GVNGFNLIFGNRVLAGSAIGGLAETQEMLDFCAEKNVLPDVEMIRADQINEAWERLKK
                     GDVRYRFVIDMSSLADAQ"
     gene            1371..>3006
                     /gene="acs"
                     /locus_tag="VFU20_08260"
     CDS             1371..>3006
                     /gene="acs"
                     /locus_tag="VFU20_08260"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEU5286481.1"
                     /translation="MSEAVHPVPDGFRADIGPAELAELHRRADQDADSFWLDQARRLD
                     WARFPTRAGDWSFDKADFAIRWYADGELNLSVNCLDRHLAERGDRVALIFEPDEPGTG
                     RTLTYRELLAETCRMANLLKSRGVGRGDRVMIYMPMIPEAAAAMLACARIGAIHSVVF
                     GGFSPDSLAGRIADCDACAVITADEGVRGGRTIPLKANVDTALARRDVATVVVVTRTG
                     AEVPMRHGRDISYSAVRDELAATCEPEVMNAEDPLFILYTSGSTGQPKGVVHTIGGYA
                     VWAAATFDWIFGPSDDDIFWCTADVGWITGHSYVVYGPLINGATSVMFDGVPNYPDFG
                     RFWETVDRLGVTIFYTAPTAIRALMREGDDWVKRHSRRSLRLLGTVGEPINPEAWEWY
                     WRVVGDGRCPVTDTWWQTETGGILIAPFPGATPMKPGSATRPLPGIKPLLVDAEGKEL
                     HGPASGNLCIAASWPGQMRTVWGDHDRFFATYFTTYPGRYFTGDGCKRDADGYYWITG
                     RVDDVMNVSGHRLGTAEVESALVSHAKVAEAAVVGFP"
BASE COUNT          470 a         1063 c         1033 g          440 t
ORIGIN      
        1 cggcggccgg agtgcagccg gtcgacgggg tgttcgccga cttcaaggac ccaaaagggc
       61 tgcgctccga atccgaggcc gcgcgccgcg agggattcac cggcaagctc gccatccacc
      121 ccgaccaggt cgcaatcatc aacgccgcct tcacaccgtc caaggccgag atcgcgcacg
      181 cccgggcgat cgtcgccgca ttcgaggcga agcccggcgc gggagtgctt tcggtcggcg
      241 gccggatggt cgaccgaccg cacctgctgc aggcgcagag gatcctcgcc cgcgcccaat
      301 agaggaggcc cgacgatgac gacgacagcc agagcatggg gagtcaccgg cgccgaatcg
      361 cccgtggtcc cgctgaccat cgagcgccgc gagcttcgcc ccgaggacgt cgcgatcagg
      421 atcagccatt gcggcatctg ccacaccgac ctgcacttcg cccacaacga ctggggaatg
      481 agcgaatatc cgatggtccc ggggcacgag atcgtcggca ccgtcaccgg cgtcggcagc
      541 ggggtcacgc ggtaccaggc cggcgaccgg gtcgcggtcg gctgcctggt cgacagcgac
      601 ctcagctgcg agcaatgccg cgccggctac gagcaattct gcgtcgacat gccgaccttc
      661 acctacagcg gccgcgaccg ccagagcggc gagctcaccc agggcggcta ttcggaccat
      721 gtgatcgtcc ggcaggagtt cgtctgccgg gttccggaca gcttcgacat ggcccgcgcg
      781 gcgccgttgc tgtgcgccgg gatcaccact tactcccccc tgcgacgctt caaggtcggg
      841 cccgggcagc gggtcgcggt ggtcgggctg ggcggcctcg ggcacatggg agtcaagctc
      901 gcggcggcga tgggcgccca cgtgacgatg gtcaccactt cgccggccaa gggcgaggac
      961 gcgcgccagc tcggcgccga cgaggtgctg gtctcgaccg acccggaggc gatggcggcg
     1021 gccgccgggc gcttccattt cgtcctcgac accatcccgg tcgcgcacga ggtccagccc
     1081 tatctcgacc tcctcgggcc gcacggagcg ctggtgctgg tcggggcgct cgagccgctt
     1141 cccggggtca acggcttcaa cctcatcttc ggcaaccggg tgctcgccgg ctcggcgatc
     1201 ggcggcctcg ccgagaccca ggagatgctc gatttctgcg ccgagaagaa cgtcctgccc
     1261 gacgtcgaga tgatccgcgc cgaccagatc aacgaggcct gggagcggct gaaaaaaggc
     1321 gacgtgcgtt atcgcttcgt gatcgacatg agcagcctgg ccgacgccca atgagcgagg
     1381 cggtccatcc cgttcccgac ggcttccgcg cggacatcgg tccggccgag ctagccgagc
     1441 ttcaccgccg cgccgaccag gacgccgact ccttctggct cgaccaggcg cggcggctcg
     1501 actgggcgcg cttcccgacg cgggccggcg actggtcgtt cgacaaggcc gatttcgcaa
     1561 tccgctggta cgccgacggc gaactgaacc tgtcggtcaa ttgcctcgac cggcacctgg
     1621 cggagcgcgg cgaccgggtg gcgctgatct tcgagccgga cgagccgggc accgggcgca
     1681 ccctcaccta ccgcgagctc ctcgccgaga cctgccggat ggcgaacctg ctcaagtcgc
     1741 gcggcgtcgg ccgcggcgac cgggtgatga tctacatgcc gatgatcccc gaggcagcgg
     1801 cggcgatgct cgcctgcgcc cggatcggcg cgatccactc ggtggtgttc ggcggcttct
     1861 cgcccgacag cctcgccggc cgcatcgccg actgcgacgc ctgcgcggtg atcaccgccg
     1921 acgagggcgt tcgcggcggc aggacaatcc cgctgaaagc caatgtcgac accgcgctgg
     1981 cccggcgcga cgtcgcaacg gtggtcgtcg tcacccggac gggcgccgag gtgccgatga
     2041 ggcacggccg cgacatctcc tattcggcgg tgcgcgacga actggcggcg acatgcgagc
     2101 ccgaagtcat gaacgccgag gacccgctgt tcatcctcta cacgtcgggc tcgaccggcc
     2161 agcccaaggg cgtcgtccac accatcggcg gctatgcggt gtgggcggcg gcgaccttcg
     2221 actggatctt cgggccaagc gacgacgaca tcttctggtg caccgcggac gtcggctgga
     2281 tcaccggcca cagctatgtc gtctacggcc cgctgatcaa cggcgcgacc agcgtgatgt
     2341 tcgacggggt ccccaactat cccgacttcg gccgcttctg ggagaccgtc gaccgattgg
     2401 gcgtcaccat cttctacacc gcgccgaccg cgatccgggc gctgatgcgc gagggcgacg
     2461 attgggtgaa gcgccactcg cgccggtcgc tgcggctgct cggcaccgtc ggcgagccga
     2521 tcaaccccga ggcgtgggaa tggtattggc gggtcgtcgg cgacgggcgc tgcccggtca
     2581 ccgacacctg gtggcagacc gagaccggcg gcatattgat cgcgcccttc cccggagcga
     2641 cgccgatgaa gcccgggtcc gcgacccggc cgctgccggg gatcaagccg ctgctggtcg
     2701 atgccgaagg caaggagctc cacgggcccg ccagcggcaa tctgtgcatc gccgcatcct
     2761 ggcccggcca gatgcgcacc gtgtggggcg accacgatcg ctttttcgcc acgtatttca
     2821 cgacttatcc cgggcgctat ttcaccggcg acggctgcaa gcgcgacgcc gacggctact
     2881 actggatcac cggccgcgtg gacgacgtga tgaacgtgtc cggccatcgc ctgggcacgg
     2941 cggaagtgga gagcgcgctg gtctcgcatg ccaaggtcgc ggaggcggcg gtggtcggct
     3001 tcccgc
//
LOCUS       DASTZO010000294         5875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_777249,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000294 DASTZO010000000
VERSION     DASTZO010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5875
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777249"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VFU20_08265"
     CDS             <1..300
                     /locus_tag="VFU20_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5286482.1"
                     /translation="RRRYTNVWKLYEWVAGHPVDDPPIPDKEEKPPVPTLAIWSRRDG
                     IVGAPAARGTEETRDKAVEVDSTHMGFAVSRKGTRRAVAEIVRFLKEVEGAESRH"
     gene            359..1069
                     /locus_tag="VFU20_08270"
     CDS             359..1069
                     /locus_tag="VFU20_08270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286483.1"
                     /translation="MAKGLSLVRGGAGAPAALHRLPNGSGERWGLPAPPGPTLCELFA
                     AHPRDGGWAGFLLAQLEPGKPLLWVQERMALLEAGRVYPPGIDCRDILHVTARDARSA
                     LWAMEEGLRCSALGAVIGELWGDPASLDFTATRRLAVAAERSGVPAWLVRLGGAANLS
                     GARMRWRVASRPSLAHPFDPRAPGAPAWDAELFRARGMPPGRWNVAHDTAAAHPFRLV
                     AAAGDRALEADERERLSA"
     gene            978..2552
                     /locus_tag="VFU20_08275"
     CDS             978..2552
                     /locus_tag="VFU20_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HEU5286484.1"
                     /translation="MTQPQRILSVWWPQLAIERWKRTSGNGFPPDSPVVLTVEGAHGP
                     VIAAVSRAAAECGARAGARLTDARALEPGLVAVPADPAGDSALVARLARWAARWSPLV
                     EVDGGDALRLDVTGVAHLFGGESALVEDVRSRFAALGLTCRVAIAPTPGAVWALARYG
                     KNTLTLPLRGSLPLPRAGEGWGEGLDLLAPLPVAALRLSSDAVRTLERLGLKTIGALA
                     GVPRRALARRFRAADNVLDALDRALGRKPEPLVAAPCEPPPRSLLRLEEPATHPEAAP
                     QALERLIPDLVAQLEQRRLGARRLALTGFRVDGSIAVAGVATSIPSREPKHLQRLLAD
                     QAAMLDPEFGFDGFALESDWTEPLGAAQDSLVEEPSGIGDIARLIDRLSVKLGPERVR
                     RPRARESHLPERASGWVAALEDRPSPQPSPVNGRGSRPQRLLDRPEAIAVIYATPEGV
                     PRRFVWRRSMHDIARVEGPERIAPEWWRAPSNSRLRDYYRVEDSAGRRFWIYREGVIG
                     DGRGGAPAWYLHGLFG"
     gene            2552..5833
                     /locus_tag="VFU20_08280"
     CDS             2552..5833
                     /locus_tag="VFU20_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017664056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HEU5286485.1"
                     /translation="MPEYSNFARKKLVRDGPVEPIPRARFVELGVTSPFSFLRGASDA
                     IELVLTALELGMDSIGIADRNSLAGVVRMHSAAKAAGLRPLIGCRLDLSDAPSLLAYP
                     RDRDAYGRLSRLLSLGKMRAKKGECELSLADVAGHQDGIAFIAWPEDLDEFERQLPRL
                     KEALPSLRHVAASYLYRGDDSARIERLDRIARANGAAILATNDIHYHAPDRRPLQDVM
                     SCIREKVTIASAGYLLNPNAERHLKSPAVMAGLFARWPQAIESTREFADALDFSLDEL
                     AYEYPLETVPDGRTPQQHLEHLTREGARQRWPGGVPAKVEQQLRHELELIRRLDFARY
                     FLTVHDIVAFARSLDPPILCQGRGSAANSAVCFCLGITAVDPATSDLLFERFISEERR
                     EPPDIDVDFEHERREEVIQHIYSKYGRERAGIAATVIHYRPRSAIREVGKVMGLSEDV
                     TAALTSTIWGQMGDEVVAERAADAGLDLSDPHLKRTVKLAQQLIGMPRHLSQHVGGFI
                     LTERPLTETVPVGNGAMDERTFIEWDKDDIDELGILKIDVLALGMLTCIRKAFDLIER
                     HHGERWELATVPPEEPGVYDMLCRGDSLGVFQVESRAQMNMLPRLKPRCFYDLVIEVA
                     IVRPGPIQGDMVHPYLKRRSGKQPVLYPCPAPEHGPPDELERILKRTLGVPVFQEQAM
                     KIALDAARFTPAEANQLRKAMATFRSRGTIELLQDKMVGRMIGRGYDPDFAQRCFDQI
                     KGFGEYGFPESHAASLAHLVYVSSWLRWRYPAAFACALLNSQPMGFYAPAQIVRDARE
                     HGVEVRPVDVNHSDWDCTLENGALRLGLRQVEGLRSDSADRLVAARGTRAYASVEDVR
                     TRGQVPVHAIERLAAADAFRSMKLDRRAALWDSRALKAAPDLPLFAAADARDEGAEAE
                     PATLPAMPLSEHVVTDYRTVRLSLKAHPMRFLREHYARRRFVTARDLESVRNGKRVSI
                     AGLVLIRQRPGSAKGVVFITIEDETGVANLIVWPDVFATQRKTVMGARLMAVHGLVQK
                     DEDDGVIHVIARRLEDDTPMLRQLSEDIMDAPLSRGDAPGAMRPPPGRHPRDAEVIPK
                     SRDFH"
BASE COUNT          850 a         2007 c         2147 g          871 t
ORIGIN      
        1 cggcgccgct acaccaacgt gtggaaattg tacgaatggg tcgccggcca cccggtcgac
       61 gacccgccga tcccggacaa agaggagaag ccgccggtgc cgaccctggc gatctggtcg
      121 cggcgcgacg ggatcgtcgg agcgcccgcc gcgcgcggca cggaggagac gcgcgacaag
      181 gcggtggagg tcgattcgac ccacatgggc tttgcggtgt cgaggaaggg gacccggcgg
      241 gcggtggccg agatcgtgcg ttttctgaag gaagtggaag gggccgagtc gcgccattga
      301 ctcttggccc cgagtcgcgg gacaagaaca aacaaagaac aaaagcgttg tgagtcgtgt
      361 ggcgaaaggc ttgtccctgg tccgcggcgg cgcgggcgcg ccggccgcac ttcatcgtct
      421 ccccaacggg tcgggcgagc gctggggcct gcccgcgccg cccggcccga ccctgtgcga
      481 gctgttcgcc gcccacccgc gcgacggcgg ctgggccggg ttcctgctcg cccagttgga
      541 gcccggaaag cctttgctgt gggtccagga gcggatggcg cttctcgagg cggggcgggt
      601 ctatccgccg gggatcgact gccgcgacat cctccacgtc accgcgcgcg acgcccgctc
      661 ggcgctgtgg gcgatggagg aggggctgag gtgctcggcg ctcggagccg tgatcggcga
      721 attgtggggc gacccggcga gcctcgactt caccgccacc cggcggctgg cggtcgcggc
      781 cgagcggtcg ggcgttccgg cctggctggt gcggctgggc ggcgcggcca acctcagcgg
      841 cgcccggatg cgctggcggg tcgccagccg gccgagcctt gcccacccgt tcgacccgcg
      901 cgcgcccgga gcgccggcgt gggacgccga gctgttccgc gcgcgtggaa tgccgcccgg
      961 ccggtggaac gtcgcccatg acacagccgc agcgcatcct ttccgtctgg tggccgcagc
     1021 tggcgatcga gcgctggaag cggacgagcg ggaacggctt tccgcctgac tcgccggtcg
     1081 ttctgacggt cgagggcgcg cacgggccgg tgatcgcggc ggtgagccgg gcggcggcgg
     1141 aatgcggggc gcgggccggc gcccggctga ccgatgcgcg ggcgctcgag ccggggctgg
     1201 tcgccgtccc cgccgatccc gccggggact cggcgctggt cgcaaggctc gcgcgctggg
     1261 cggcgcgctg gtcgccgctg gtcgaggtcg acggcggtga cgcgctccgc ctcgatgtga
     1321 ccggcgtcgc gcatctgttc ggcggcgagt cggcgctggt cgaggatgtc cgcagccggt
     1381 tcgccgcgct cggcctgacg tgccgggtcg cgattgcccc gacccccggc gcggtctggg
     1441 cgctggcgcg gtacggaaag aataccctca ccctcccgct tcgcgggtcc ctccctctcc
     1501 cgcgcgcggg agagggttgg ggtgagggtc ttgaccttct cgcgccgctg ccggtcgcgg
     1561 cgctgcggct gtcgtccgac gcggttcgga ccctcgagcg gctcggcctc aagaccatcg
     1621 gcgcgttggc cggggtgccg cgccgggcgc tcgcccgccg cttccgcgcg gccgacaatg
     1681 tgctggacgc gctcgaccgg gcgctgggcc gcaagcccga gccgctggtc gcagccccgt
     1741 gcgagccgcc gccgcgcagc ctgctgcggc tcgaggagcc ggcgacccat ccggaggccg
     1801 cgccccaggc gctcgagcgg ctgatccccg atctcgtggc gcaactggag caacgccgtc
     1861 tcggcgctcg gcggctggcg ctgaccggct tccgggtcga tgggagcatc gcggtcgccg
     1921 gcgtcgccac gtcgatcccg agccgcgagc cgaagcatct ccagcgcctg ctcgccgacc
     1981 aggctgcaat gctcgatccg gagttcggct tcgacggttt cgcgctcgag tcggactgga
     2041 ccgagcccct gggcgcggcg caggacagcc tggtcgagga accatcgggg atcggcgaca
     2101 tcgcccgcct gatcgaccgc ctgagcgtca agctcgggcc ggagcgggtg cgtcggccgc
     2161 gcgcccgcga aagccatctg cccgagcgcg cgagcgggtg ggtggcggcg ctcgaagaca
     2221 ggccctcacc ccaaccctct cccgtaaacg ggagagggag tcgcccccag cgcctgctcg
     2281 accggccgga agcgatcgcg gtcatctatg cgacccccga gggggtgccg cggcggttcg
     2341 tgtggcggcg ctcgatgcac gacatcgccc gggtcgaggg gcccgagcgg atcgcccccg
     2401 aatggtggcg ggcgccgtcg aacagccgcc tgcgcgatta ttaccgagtc gaggattcgg
     2461 ccggccgccg cttctggatc taccgcgagg gtgtgatcgg agatggccgc ggcggagcgc
     2521 ccgcctggta cctgcacggg ctcttcggct gatgcccgaa tatagcaact tcgcccgcaa
     2581 gaagctggtc cgcgacgggc cggtcgagcc gatcccgcgg gcgcggttcg tcgagctcgg
     2641 ggtgaccagc cccttctcgt tcctgcgcgg ggcatcggat gcgatcgagc tggtgctgac
     2701 ggcgctcgag ctcggcatgg actcgatcgg gatcgccgac cgcaacagcc tcgccggggt
     2761 ggttcggatg cacagcgccg ccaaggccgc ggggctgagg ccgctgatcg gctgccgcct
     2821 cgaccttagc gatgcgccga gcctgctcgc ttacccacgc gaccgcgacg cctacggccg
     2881 gctgtcgcgg ctgctgagcc tgggcaagat gcgcgcgaag aaaggcgagt gcgaattgag
     2941 ccttgccgac gtcgccgggc accaggacgg aatcgccttc atcgcctggc cggaggattt
     3001 ggatgagttc gaaaggcagc tgccaagact gaaggaagcc ttgccgtcgc tccgccacgt
     3061 cgccgcgtcc tatttgtacc gcggcgacga ttcggcgcgg atcgagcggc tcgaccggat
     3121 cgccagggcc aatggcgccg cgatcctcgc gaccaacgac atccattacc acgcgcccga
     3181 ccgccgcccg cttcaggacg tgatgagctg catccgcgag aaggtcacca tcgccagcgc
     3241 cggttacctt ctcaacccga atgcggagcg gcatttgaag agcccggcgg tgatggccgg
     3301 attgttcgcc cgctggccgc aagccattga atcaacgcgg gagtttgccg acgcgctcga
     3361 cttcagcctc gacgaattgg cttacgaata tccgctcgag acggtgcccg acgggcgcac
     3421 gccgcagcaa catctcgagc atctgacccg cgagggagca cggcagcgct ggccgggcgg
     3481 cgtccccgcc aaggtcgaac agcagctgcg ccacgagctc gagctgatcc gccggctgga
     3541 cttcgcgcgc tacttcctga ccgtccacga catcgtcgcc ttcgcgcgca gcctcgaccc
     3601 gccgatcctg tgccaggggc gcgggtcggc ggccaacagc gcggtctgct tctgcctcgg
     3661 gatcaccgcg gtcgacccgg cgaccagcga ccttttgttc gagcgcttca tctccgagga
     3721 gcgcagggag ccgcccgaca tcgacgtcga tttcgagcac gagcggcgcg aggaggtgat
     3781 ccagcacatc tattccaaat acgggcgcga gcgggccggg atcgccgcga ccgtgatcca
     3841 ctaccgcccg cgcagcgcga tccgcgaggt cggcaaggtg atgggcctca gcgaggatgt
     3901 gaccgcggcg ctcacctcga ccatctgggg gcagatgggc gacgaggtcg tggccgagcg
     3961 cgcggccgac gccgggctcg atttgagcga cccgcatctg aagcgcacgg tcaagctcgc
     4021 gcagcaattg atcggcatgc cgcggcattt gtcgcagcac gtcggcggct tcatcctcac
     4081 cgagcggccg ctgaccgaga cggtgccggt cggcaacggc gcgatggacg agcgcacctt
     4141 catcgaatgg gacaaggacg acatcgacga gctcgggatc ctcaagatcg acgtgctggc
     4201 gctcggcatg ctgacctgca tccgcaaggc gttcgacctg atcgagcggc atcacggcga
     4261 gcgctgggag ctggcgaccg tcccgccgga ggagccgggc gtctacgaca tgctgtgcag
     4321 gggcgattcg ctgggcgtgt tccaggtcga aagccgggcg cagatgaaca tgctgccgcg
     4381 attgaagccg cgctgcttct acgatttggt gatcgaggtc gcgatcgtcc gcccgggccc
     4441 gatccagggc gacatggtcc atccatatct caagcgccgc agcggcaagc agccggtgct
     4501 ctatccctgc cccgcccccg agcacggccc gcccgacgaa ttggagcgga ttttgaagcg
     4561 gacgctcggc gtgccggtgt tccaggagca ggcgatgaaa atcgcgctcg acgcggcgcg
     4621 gttcaccccc gccgaggcca accagttgcg caaggcgatg gcgaccttcc gctcgcgcgg
     4681 gacgatcgaa ttgctgcagg acaagatggt cgggcggatg atcggccgcg gctacgaccc
     4741 ggatttcgcc cagcgctgct tcgaccagat caagggcttc ggcgaatatg gctttcccga
     4801 gagccacgcg gcgagcctcg cgcacctggt ctacgtgtcg agctggctgc gctggcgcta
     4861 cccggcggcg ttcgcctgcg ccttgctcaa ttcgcagccg atgggctttt acgcgcccgc
     4921 gcagatcgtc cgcgacgcgc gcgagcatgg agtcgaggtg cggccggtgg acgtcaatca
     4981 ctcggactgg gactgcactc tggagaatgg tgcgctgcgg ctcggcctgc ggcaggtcga
     5041 ggggctccgc tcggactcgg ccgatcggct ggtcgcggcg cgcgggacgc gggcttatgc
     5101 gagcgtcgag gacgtccgca cgcgcggcca ggtgccggtg cacgcgatcg agcggctggc
     5161 ggcggccgac gcgttccggt cgatgaagct cgaccgccgc gcggcgctgt gggattcgcg
     5221 cgcgctcaag gcggcgccgg acctgccctt gttcgctgcg gcggatgcgc gcgacgaggg
     5281 cgccgaagcc gagcccgcga cgcttccggc gatgccgcta tccgagcatg tggtgaccga
     5341 ttaccggacg gtgcggctga gcctgaaggc gcacccgatg cgcttccttc gcgagcatta
     5401 tgcgcggcgc cgcttcgtca ccgcgcgcga cctggaatcc gttcgcaacg ggaaacgcgt
     5461 gtcgatcgcc ggactggtgc tgatccgcca gcggccgggc agcgccaagg gcgtggtgtt
     5521 catcaccatc gaggacgaga ccggcgtggc caacctaatc gtctggccgg atgtgttcgc
     5581 aacgcagcgc aagaccgtca tgggggctcg gctgatggcg gtccatgggc ttgtccagaa
     5641 ggacgaggac gacggcgtga tccacgtgat cgcccggcgc ctggaggacg acacgccgat
     5701 gcttcgccag ctgagcgagg acatcatgga cgcgccgctg tcgcgcggcg atgcgccggg
     5761 agcgatgcgc ccgccgcccg gccgccaccc gcgcgacgcc gaggtgatcc ccaagtcgag
     5821 ggatttccac tgattcccct ccccttgatg gggaggggta ggggtagggt gacgg
//
LOCUS       DASTZO010000295         6088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_358302,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000295 DASTZO010000000
VERSION     DASTZO010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6088
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358302"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..1493)
                     /locus_tag="VFU20_08285"
     CDS             complement(171..1493)
                     /locus_tag="VFU20_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5286486.1"
                     /translation="MAATETRETLGWRASLPAGVRPYTEAAPIAAFFLGVSSGFPYAM
                     IGATLTTRLAQDDITKSTVTAFALAFLVYNLKFLWAWVVDGVRLPLLGRLGQRVSWLI
                     FAGMLVIAAVANLAFQDPSANIVQTAVAAILVGIAGATFDIVIDGYRIELLEPRQLGV
                     GSGMSQYGWRIGSVAAGALALVLAARVGWEAAYLACAAFALPAMITGLVMGEPRRHRE
                     PSGKQGLAETWAAIVGPFAEFFKRSGAWLVLLFILLHKIGDTLANLVLRLLLDDMGYT
                     NDEIAIYDVGFGFWAFLIGIFVGGILYSQMGMKRSVLLSLVLMGVSNVSFAALAAAGH
                     TNWGLAATIGFENFASGIGGVVVVAYFSALCNLRFTATQYALISAAASVVGRLLTGTT
                     AGALIETMGYVNFYLMTTLIACPGVFLYWYMIRSGLADVSIGSAGQAD"
     gene            complement(1573..2004)
                     /locus_tag="VFU20_08290"
     CDS             complement(1573..2004)
                     /locus_tag="VFU20_08290"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5286487.1"
                     /translation="MLFGKRKRIVKRILVVEDEPLTAFDNETMLIDAGYEVVATLDGF
                     DDALEVIEREKIDLILSDIRLRSHETGIDLAAAAKKRGIPTLFASGHAMPAAAEVAVG
                     CLKKPYTERQLRAAIDSVDRHLAGERVKPPSGLDIFIREQG"
     gene            2104..2850
                     /locus_tag="VFU20_08295"
     CDS             2104..2850
                     /locus_tag="VFU20_08295"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU5286488.1"
                     /translation="MSKRPEPRETGPQRIAKLLARAGVASRRDVERMIAEGRIARNGE
                     TLATPATLLDDLAGVTVDGKPVRPPSAPRLFRFYKPPATLTAERDPKGRPTIYDRLPK
                     SLPRLIPVGRLDFMTEGLLLLTNDGELKRQLELPSSGVVRHYRARAFGSVTQAQLEDL
                     SEGVTIEGMRYGSIDANLERRTGRNCWIQLSLAEGKNREVRRVLEHLGLQVSRLIRTG
                     YGPFDLAGLEPGEVREIDRNELAHFRNSLK"
     gene            2847..3386
                     /gene="rsmD"
                     /locus_tag="VFU20_08300"
     CDS             2847..3386
                     /gene="rsmD"
                     /locus_tag="VFU20_08300"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU5286489.1"
                     /translation="MRIISGEWRGRRLRAPPGVETRPTADRVRETLFSMLASRLGSLQ
                     GLRVADLFAGSGALGLEALSRGAALACFVETDAKAAAAIRANAADLGALERVQILGGS
                     ALALPRSEPFDLLFADPPYAPGSGSAAVRAVAAAGWLAPGGWMAVETARNDAVDPGGW
                     AIDAARTVGRAKLTLLRVP"
     gene            complement(3364..3771)
                     /locus_tag="VFU20_08305"
     CDS             complement(3364..3771)
                     /locus_tag="VFU20_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286490.1"
                     /translation="MARKSSPAKPGATARKKAAAKPKSRTAARKPARSAAGADRRKRA
                     GRRSSDLTAADALFKLIESPLVADLLAVGATAALAAIAESRFSRKGDEGRKSSRALKA
                     AAKAAAAAMGRRLSTEVEEIRKASKKPKGPAKA"
     gene            complement(3804..4403)
                     /locus_tag="VFU20_08310"
     CDS             complement(3804..4403)
                     /locus_tag="VFU20_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEU5286491.1"
                     /translation="MCNLYSMTATVDELRRIFGPFDGDTANLPPFDEIYPGYQAPVLR
                     RKDNGGLKLEMMKWGFPGPAAAGSRPVTNVRNLSSPFWRNALNDPARRCIVPATRFCE
                     WTAEPDPETKRKAKVWFGLADEHPALFAFAGLWRPGEEGPYMAFLTCAANATVGAVHP
                     KAMPVMLRPADTGRWLDSERADACALAQPFEDAAMRRVG"
     gene            4500..5609
                     /locus_tag="VFU20_08315"
     CDS             4500..5609
                     /locus_tag="VFU20_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286492.1"
                     /translation="MYSQNELDEAVAAGAISAEAAASLRAYIERQHATPTVDEEQFRL
                     LTGFNDIFVAIAAAILLFSVAWIGQRIGQGLGLAIEGEGPSPFGPLAVAATSWALALF
                     FTARRRMALPSILLLLAFVGSVFAAAAFTLVLAIGPERLEDNVQLAGVLAAVSGGIAA
                     GAAWLHWKRFRVPITVAAGAAAVAAIAVGLLIAALGDNADTARDLILGFVLLLGIGTF
                     LFAMSWDSSDRARITRRSDVAFWLHLLAAPMIVHPVFTLLGLNDGNATIGEGLVVVLL
                     YVVIGITALAIDRRALLVSALAYVLWALSDLFERFGAVELNIALTALVIGSALLLLSA
                     FWSQARAAVVRPLPEGLRERLPIVNRSAVAPQAAA"
     gene            complement(5606..5947)
                     /locus_tag="VFU20_08320"
     CDS             complement(5606..5947)
                     /locus_tag="VFU20_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HEU5286493.1"
                     /translation="MSVVAPFPHPRDRRLIASFDRRELTRILDLYGRMVAAGHWRDYA
                     IRFAPDAAVFAAFRRSSERPEVRIEKRPELRLKQGAFALVGEHGQVLKRGPDLGLVLA
                     PVERRLMRLID"
BASE COUNT          886 a         2193 c         2069 g          940 t
ORIGIN      
        1 ctacctcaag gacgttgacg gccgacaagc tgccggccgt cgcgggtttg actcttcgcg
       61 gattcgacga cggcgatcgc cgtaccacgc acggcggcgg catctggtcg atcgcgcagc
      121 cgtagcaacg ggctggaccg cgtcgccgtc gagcggacag cgcgcgtggc tcagtccgct
      181 tgccccgccg acccgatcga gacatccgcc aggcccgatc gaatcatgta ccagtagagg
      241 aacacgccag gacaggcgat cagggtcgtc atcaggtaga agttgacgta gcccatggtc
      301 tcgatgaggg cgccggcggt ggtcccggtc agcagccggc cgacgacgct cgccgccgcc
      361 gagatcaatg catattgggt ggcggtgaag cgcagattgc acagcgccga gaagtaagcg
      421 acgaccacca ccccgccgat gccgctggcg aaattctcga agccgatggt ggccgcgagc
      481 ccccagttgg tgtggccggc ggcggccagc gccgcgaagc tcacattcga cacgcccatc
      541 agcaccagcg acaggagcac cgaccgcttc atccccattt gcgaatagag gatgccgccg
      601 acgaagatgc cgatcaggaa cgcccagaag ccgaagccga cgtcgtagat cgcgatctcg
      661 tcgttggtgt agcccatgtc gtcgagcagc agccgaagca cgaggttggc gagcgtgtcg
      721 ccgatcttgt gcaaaaggat gaacagcagc accagccagg cgccgctgcg cttgaagaac
      781 tcggcgaacg ggccgacgat cgccgcccag gtctccgcga ggccctgctt gccgctcggc
      841 tcgcggtgcc gccggggctc gcccatgacg aggccggtga tcatcgccgg aagggcgaag
      901 gcggcgcagg cgaggtaagc ggcttcccag ccgacccgcg cggccagcac cagcgccagc
      961 gctccagcgg cgaccgagcc gatccgccag ccatattgcg acatgcccga gccgaccccg
     1021 agctggcgcg gctcgagcag ctcgatccgg tagccgtcga tgacgatgtc gaacgtcgct
     1081 ccggcgatcc cgaccaggat cgccgcgacc gcggtctgga cgatgttggc ggacgggtcc
     1141 tggaacgcga ggttggcgac cgcggcgatc accagcattc cggcgaagat cagccacgac
     1201 acgcgctggc cgagccggcc gagcagcggc agtcggaccc cgtcgaccac ccaggcccag
     1261 aggaatttga ggttgtagac caaaaaggcg agcgcgaagg cggtgacggt cgacttggtg
     1321 atatcgtcct gggcgaggcg cgtcgtcagc gtcgcgccga tcatcgcata gggaaagccc
     1381 gacgagacgc cgaggaagaa agcggcgatc ggcgccgcct cggtgtaggg ccggaccccg
     1441 gcgggcaggc tggcgcgcca gccgagggtt tcacgagtct cggtagccgc catgcctcac
     1501 cctccatttc gacattcggc cgccatgcta gcagcggatc ggcggctgaa cagtgggcaa
     1561 tgagtggacc gctcagcctt gctcgcggat gaagatgtcg aggccgctcg gcggcttgac
     1621 gcgctcgccc gccaggtggc ggtcgacgct gtcgatcgcc gccctcagct ggcgctcggt
     1681 gtagggcttc ttgaggcagc cgaccgcgac ctcggcggcc gccggcatcg cgtgcccgct
     1741 ggcgaacagg gtcgggatcc cgcgcttctt ggccgccgcc gcgaggtcga tgccggtctc
     1801 gtggctgcgc aggcggatgt cactcaggat caggtcgatc ttctcgcgct cgatcacttc
     1861 gagcgcgtcg tcgaagccgt ccagggtcgc caccacctca tagccggcat cgatcagcat
     1921 cgtctcattg tcgaacgcgg tcagcggctc gtcctcgacg acgaggatcc gcttgacgat
     1981 ccgcttgcgc ttgccgaaca gcatccagac ttttccaagc caaaattttc caaggactgg
     2041 cacttgcccc cgcaacgaac gaaccccgat cgagtgccgc ggcgccgcgg cgcccggcga
     2101 acgatttcga aacggcccga gccgcgcgag accgggccgc agcggatcgc caagctgctc
     2161 gcccgcgccg gggtcgcgtc gcgccgcgac gtcgagcgga tgatcgccga gggccgaatc
     2221 gcccggaacg gcgaaaccct ggccactccg gcgacgctgc tcgacgatct ggccggggtg
     2281 accgtcgacg gcaagccggt ccggccgccg tcggcgccgc gactgttccg cttctacaag
     2341 ccgccggcga ccctgaccgc cgagcgcgac cccaagggcc ggccaacgat ctacgaccgg
     2401 ttgcccaaaa gcctgccgcg gctgatcccg gtcggccgcc tcgacttcat gaccgagggg
     2461 ctgctgctct tgaccaacga cggcgaattg aagcgccagc tcgagctgcc gagcagcggc
     2521 gtcgtccgcc attaccgcgc gcgcgcgttc ggcagcgtca cccaggccca gctcgaggat
     2581 ttgtccgagg gggtgaccat cgagggcatg cgctacggct cgatcgacgc caacctcgag
     2641 cggcgcaccg gccgcaactg ctggatccag ctgagcctgg ccgaaggcaa gaaccgcgag
     2701 gtccggcggg tgctcgagca tctcggcctg caggtgtcgc gcctgatccg caccggctac
     2761 ggcccgttcg acctcgccgg cctcgagccg ggggaagtcc gcgagatcga ccgcaacgag
     2821 ctcgcgcact tccgcaacag cctcaagtga ggatcatatc cggagagtgg cgcgggcgcc
     2881 ggctccgggc gccgccggga gtcgagaccc gcccgaccgc cgaccgagtg cgcgaaaccc
     2941 tgttctcgat gctcgccagc cgcctcggct cgttgcaggg cttgcgcgtc gccgacctgt
     3001 tcgccggcag cggagcgctg gggctggagg cgctgtcgcg cggcgctgcc ctcgcctgct
     3061 tcgtcgagac cgacgccaag gccgcggcgg cgatccgcgc caacgcggcc gatctcggag
     3121 cgctcgagcg ggtgcagatc ctcggcggct cggcgctcgc attgcccagg tccgagccgt
     3181 tcgacctact gttcgccgac ccgccctacg cgccgggctc gggcagtgcg gccgttcggg
     3241 cggtcgccgc cgcgggctgg ctcgcgccgg gcggctggat ggcggtcgag accgcgcgca
     3301 acgacgccgt cgatcccggc ggctgggcca tcgacgcggc ccgcaccgtc ggccgggcca
     3361 agcttacgct tttgcgggtc ccttaggctt tttcgacgcc ttgcggattt cctcgacctc
     3421 ggtcgacagc cgccgcccca tcgccgccgc cgccgccttg gccgccgcct tcagcgccct
     3481 gctcgacttg cgcccttcgt cgcccttgcg gctgaagcgg ctctcggcga tcgccgccag
     3541 ggccgcggtc gcgccgaccg cgagcaggtc ggcgaccagc gggctttcga tcagcttgaa
     3601 cagggcatcg gccgcggtca ggtcgctcga ccggcgcccg gcccgcttgc ggcgatccgc
     3661 acccgccgcc gagcgcgccg gcttgcgcgc cgcagtcctc gatttcggct tcgccgccgc
     3721 tttcttccgc gccgtcgcgc cgggctttgc cgggcttgac ttccttgcca tctcttccct
     3781 cctgttttct cgtctcgctc agcttaaccc acacggcgca tcgccgcatc ctcgaatggc
     3841 tgcgcgagcg cgcaggcgtc ggcgcgctcg ctgtcgagcc agcggccggt gtcggcgggc
     3901 cgaagcatca ccggcatcgc cttggggtgc accgctccca ccgtcgcatt ggccgcgcag
     3961 gtcaggaacg ccatgtaggg cccctcctcg cccggccgcc acagcccggc gaaggcgaac
     4021 agcgccggat gctcgtcggc caggccgaac cacaccttcg ccttgcgctt cgtctcgggg
     4081 tcgggctcgg cggtccattc gcagaaccgg gtcgccggga cgatgcagcg gcgagccgga
     4141 tcgttgagcg cgttgcgcca gaacgggctg ctcaaattgc ggacgttggt caccggccgc
     4201 gatcccgccg cggccggccc cgggaagccc cacttcatca tctcgagctt gaggccgccg
     4261 ttgtccttcc ggcgcagcac cggcgcctgg tagccggggt agatttcgtc gaacggcggc
     4321 agattggccg tgtcgccgtc gaacgggccg aagatccgcc gcagctcgtc gaccgtcgcc
     4381 gtcatgctgt agaggttgca catcggctcg cgcttaccag ccccgccccg ggtgtgctac
     4441 tgtattgaca agataataca cctggcctat cgccgccgcg acagtggagg aacgggccaa
     4501 tgtactctca gaacgaactc gacgaagcgg tggcggcggg cgcgatcagc gccgaggccg
     4561 cggcgtcgct gcgcgcgtat atcgagcgcc agcacgcgac cccgaccgtc gatgaagagc
     4621 aattccggct gctgaccgga ttcaacgata ttttcgtcgc catcgccgcg gcgatcctgc
     4681 tgttctcggt cgcctggatc ggccagcgca tcggccaggg cctcggcctg gcgatcgagg
     4741 gcgaaggccc ctcgccgttc ggcccgctcg cggtcgcggc gacgtcctgg gcgctggccc
     4801 tgttcttcac cgcccggcgg cggatggcgc tgccgtcgat cctgctgctg ctggcgttcg
     4861 tcggcagcgt gttcgcggcg gccgccttca ccctcgtgct ggcgatcggc cccgagcggc
     4921 tcgaggacaa tgtccaactg gccggggtcc tcgccgccgt ctcgggcgga atcgccgccg
     4981 gcgcggcctg gcttcactgg aagcggttcc gggtcccgat cacggtcgcg gccggcgccg
     5041 cggcggtcgc ggcgatcgcc gtcggcctgc tgatcgcggc gctcggcgac aatgccgaca
     5101 ccgcccgcga cctaatcctc ggcttcgtgc tcctgctcgg catcggcacc ttcctgttcg
     5161 ccatgtcgtg ggacagctcg gaccgcgccc ggatcacccg ccgctcggac gtcgccttct
     5221 ggctgcacct gctggccgcg ccgatgatcg tccacccggt cttcaccctg ctggggctga
     5281 acgacggcaa tgcgaccatc ggcgaagggc tggtcgtggt gctgctctac gtggtgatcg
     5341 gaatcaccgc tctggcgatc gaccgccgcg ccttgctggt ctccgcgctc gcctacgtgc
     5401 tgtgggcgct gtccgacctg ttcgagcggt tcggcgcggt cgagctcaac atcgccttga
     5461 ccgcgctggt catcggctcg gcgctgctgc tcctgtcggc tttctggagc caggcgagag
     5521 ccgcggtggt tcggccgctg ccggaggggc ttcgcgagcg gctgccgatc gtcaaccgct
     5581 cggctgtcgc tccgcaagcg gccgcttagt cgatcagccg catgagccgg cgctcgaccg
     5641 gggccaggac cagaccgagg tccgggcccc gcttgagcac ctggccatgc tcgccaacga
     5701 gcgcgaacgc cccctgcttc agccggagct cggggcgttt ctcgatccgg acctcgggcc
     5761 gctcggagct tcgccggaac gccgcgaaca ccgccgcgtc gggcgcgaag cggatcgcat
     5821 aatcgcgcca atggccggcc gcgaccatcc gtccgtacag atcgaggatg cgggtcaact
     5881 cgcggcggtc gaagctggcg atcaggcgcc ggtcccgggg gtgcgggaac ggcgcgacga
     5941 cactcaagcg cttttcgcct tctgggcggg ctccttggcc gcgggctggc gcacccgctt
     6001 gagcgccgcc acctcgcgct tgagctcggc gatctcccgc tcgagctcgg cgaagccgat
     6061 ccgcgccggg tcgcattcct cgccgcac
//
LOCUS       DASTZO010000296         3216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_755952,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000296 DASTZO010000000
VERSION     DASTZO010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755952"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VFU20_08325"
     CDS             complement(<1..602)
                     /locus_tag="VFU20_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEU5286494.1"
                     /translation="MKALLSERPGGPETLRIAEVPDPKPAPGELLVRVRACAINFPDV
                     LIIEDKYQLKPPRPFAPGGEVAGEVEAVGEGVEGWAVGERLIAVPGFGGLAEKIAVPA
                     RSAYRLPAGRSFAEGAALLLTYATAIHALADRGRLRPGQTLLVLGAAGGVGLAAVELG
                     KAMGARVVAAVSSDDKAKAALEAGADDALIYPPGPFDRDGR"
     gene            complement(685..3102)
                     /locus_tag="VFU20_08330"
     CDS             complement(685..3102)
                     /locus_tag="VFU20_08330"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5286495.1"
                     /translation="MWAAIGGALSPRARFALASAALAIVYFVTAAVSMATFGTNTPIW
                     FANAFAVVWMLQVERRRWPALLFLIYIVDTAAIAVTGSGSAPVMALADVAEIFLVVSL
                     THRLGGGRAVLTSVSGLAWFILLCLGAAIPSATWGAFILWWAEGVEFVPALQQWYRSA
                     ALGLLVICPPLLIWTTPELRARITPTDARRTAVLALGLAALAALVFSQGSAAYLFVTF
                     PALLLLVWSSGLFGASVGSAVLLAVGIWETLAGGGAMATLVLPATDVALRIHALQVYL
                     VALTLSSLPLAVVLTNQRELSRQLCTVAEARSEFLAAMSHEIRTPMTGVLGIADLLEV
                     EELTPRQRNYVHSIRASGRHLLNIINDILDFSRIETGRIELEHVDFSVPMLLEELRSL
                     LHPLAAERGVELRFELAPHSPPAVKGDPTRIKQVLLNLAANAIKFTDQGSVTIAVSYE
                     HVVDGLIFRFEVQDTGIGIAADKQSELFTPFTQADHSTSRRYGGSGLGLAISKRLVAA
                     MDGEIGLRSSLGSGSLFWFEIPLQAGEATNLTAPAAKDCAPIAPCRILVAEDVELNRD
                     IVTMMLERDGHSVVLAEDGHEALAHVEQSAFDLVLMDVQMPRMDGMEATRRIRMLPGA
                     AGQVPIVALTANVMAIEQEKCLKAGMNAVLMKPIEWERLRAVVRKFGARIADPDRHGS
                     SGGEAPEADERGEPPVLVEPTFAKLKAILPRGRLKSHIEAFDAEVEVLAKAGESGDRS
                     AIQRAAHKIVSEAGSLGLARLSARAAAVEAACRDKGGLGAALRRFREAAGDSRGLLGP
                     PIRRKAG"
BASE COUNT          505 a         1157 c         1093 g          461 t
ORIGIN      
        1 cggccgtcgc ggtcgaaggg ccccgggggg tagatgagcg cgtcgtccgc gccggcttcg
       61 agcgccgctt tcgctttgtc gtccgaggag accgcggcca cgacccgggc gcccatcgcc
      121 ttgccgagct cgaccgcggc caacccgacc ccgccggccg ctccgagcac cagcagcgtc
      181 tggcccggcc gtagccgccc gcggtcggcg agtgcgtgga tcgccgtcgc ataggtgagg
      241 aggagcgccg cgccctcggc gaagctgcgc ccggccggaa gccggtaggc ggagcgggcc
      301 gggacggcga tcttttcggc cagcccgccg aagcccggaa cggcgatcag acgctcgccg
      361 accgcccagc cctcgacacc ttccccgacc gcctcgacct cccctgccac ctcgccgccc
      421 ggggcgaacg gccgcggcgg cttcaattgg tacttgtcct cgatgatcag cacgtcgggg
      481 aaattgatcg cgcaggcccg gacccggacg agcagctcgc ccggcgcggg cttcgggtcc
      541 gggacctcgg cgattcgaag cgtctcgggc ccgccgggcc gctcggacag cagggctttc
      601 agcggcgcgt ctccatcgcc tcccttatgg ggcagcaatg ccgccaagtt caaaccgctt
      661 ggcctcgggc accggcgcgc cggtctagcc cgccttgcgc cggatcggcg gccccagcag
      721 gccgcgcgag tcgccggcgg cctcgcggaa gcgacgcagc gccgcgccca gcccgccctt
      781 gtcgcggcac gccgcctcga cggccgccgc ccgggcggac agccgggcaa ggccgagcga
      841 gcccgcttcc gacacgatct tgtgcgcggc cctctggatg gccgagcggt cgccgctttc
      901 ccccgccttc gccagcactt cgacctcggc atcgaacgcc tcgatgtgcg atttcagccg
      961 gccgcggggc aggatcgcct tgagcttggc gaaggtcggc tcgaccaaca ccggaggctc
     1021 gccccgctcg tccgcctcgg gtgcctcgcc cccactgctg ccgtggcgat ccgggtcggc
     1081 gatgcgggcc ccgaacttcc ggacgaccgc cctcaaccgc tcccattcga tcggtttcat
     1141 cagcaccgcg ttcatccccg ccttcaggca tttttcctgc tcgatcgcca tgacgttggc
     1201 ggtcagcgcg acgatcggga cctggcccgc cgcgccgggc agcatccgga ttcggcgcgt
     1261 cgcctccatc ccgtccatcc gcggcatctg gacgtccatc agcaccaggt cgaacgccga
     1321 ttgctcgacg tgggccagcg cttcatggcc gtcctcggca agcaccacgc tgtgcccgtc
     1381 gcgctcgagc atcatggtga cgatgtcgcg gttgagctcg acgtcttccg cgaccaggat
     1441 ccggcacggc gcgatcggcg cgcagtcctt ggccgccggc gcggtcaggt tggtcgcctc
     1501 acccgcctgc agcggaatct cgaaccagaa caggctgccg ctgcccaggc tgctcctcag
     1561 gccgatttcg ccgtccatcg ccgcgaccag gcgcttgctg atcgccagcc cgagcccgct
     1621 gccgccgtag cgccggctgg tcgaatgatc ggcctgggtg aacggagtga acagctcgga
     1681 ctgcttgtcg gcggcgatcc cgatcccggt gtcctggacc tcgaaacgaa agatcagccc
     1741 gtcaaccaca tgctcgtagg aaacggcgat ggtgacgctg ccctggtcgg tgaacttgat
     1801 cgcattggcg gcgaggttga gaagcacctg cttgatccgc gtcgggtcgc ccttgaccgc
     1861 cggcggcgag tgcggcgcca gctcgaagcg aagctcgacc ccgcgctcgg cggcgagcgg
     1921 gtgaagcagc gagcgcagct cctcgagcag catcggcacc gagaaatcga catgctcgag
     1981 ctcgatccgg ccggtctcga tcctggaaaa atcgaggatg tcgttgatga tgttgagcaa
     2041 gtgccggccc gaggcgcgga tgctgtggac gtagttgcgc tgccgcggag tcagctcctc
     2101 cacctcgagc agatcggcga tcccgagcac gccggtcatc ggcgtccgga tctcgtggct
     2161 catcgccgcc aggaactcgc ttcgggcctc ggcgacagtg cagagctgcc gggacagctc
     2221 gcgctggttg gtcaacacga ccgccagcgg caggctcgag agggtcaggg ccaccagata
     2281 aacctgcagc gcgtggatcc tgagagcgac atcggttgcg ggtaacacca gggtcgccat
     2341 tgcgccgccg cctgcgaggg tttcccagat gccgaccgcg agcaggacgg ccgagccgac
     2401 actggctccg aacagcccgc tgctccacac caggagcagc aaggccggga atgtgacgaa
     2461 cagataggct gcgctgcctt ggctgaagac cagggcggcg agcgccgcca gtcccaacgc
     2521 aaggacagcg gttcggcggg cgtccgtcgg ggtgatccgg gcgcgcagct ccggcgtcgt
     2581 ccagatcaac agcggcgggc agatcaccag caggccaagg gcggcggacc ggtaccattg
     2641 ctgcaaggct ggaacgaact ccaccccctc ggcccaccac aggatgaatg cgccccacgt
     2701 cgcgctcggg atcgccgcgc cgaggcaaag gaggatgaac caggcgagtc cgctgacgct
     2761 tgtcaggacg gcgcgcccgc cgcccagccg gtgggtcagg ctgacgacca ggaagatttc
     2821 cgctacgtcg gccagggcca tgaccggtgc cgagcccgag ccggtgacgg cgatcgccgc
     2881 cgtgtcgacg atgtagatca ggaacagcag ggccggccat cggcggcgct ccacctgcag
     2941 catccagacc accgcgaacg cattggcgaa ccagatcggc gtgttggtgc cgaaggtggc
     3001 catcgagacg gccgccgtca cgaaatagac gattgcgagg gcggcgctgg cgagtgcgaa
     3061 tcgagcccga ggcgacaggg cgcctccaat agccgcccac atgggtcgtt ctcctttcgt
     3121 cccggcagcc gtcggcctgc cggcgacgcc aggcaagcaa aggtgggtcc gaaacggagg
     3181 ttctcgggcc gcctcgcgcc gagcggaccg gctagc
//
LOCUS       DASTZO010000297        10420 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_756027,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000297 DASTZO010000000
VERSION     DASTZO010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 10420)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10420)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10420
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756027"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VFU20_08335"
     CDS             <1..377
                     /locus_tag="VFU20_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007013792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU5286496.1"
                     /translation="EGRPLEPVSVGDYVLSGGELGAMVILDACVRLLPGVMGAASSGE
                     EESFNEGLLEYPHYTRPVTWEGRTIPEVLRSGDHARIAAWRHDRASEDTRLRRPDLIE
                     RHGGASQAPPSGARHEDEGDDQ"
     gene            374..784
                     /gene="rplS"
                     /locus_tag="VFU20_08340"
     CDS             374..784
                     /gene="rplS"
                     /locus_tag="VFU20_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU5286497.1"
                     /translation="MNLIETLEREAIDALTEMRPIPEFRPGDTLRVGVKVVEGDRSRV
                     QNFEGVCIARSNKGVGSSFTVRKISFGEGVERVFPLYSPAIDSIEVVRRGAVRRAKLY
                     YLRGRTGKSARIAERRDVRPPKGSEAAKPEKAEA"
     gene            880..2448
                     /locus_tag="VFU20_08345"
     CDS             880..2448
                     /locus_tag="VFU20_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5286498.1"
                     /translation="MKRRTLLVGVSLIALAAAGTAAYVAPLAADPVRAKDCGSALPRA
                     TSLQARAAAVPEPAWAQRGGTINDASCLSRTPVAGIVAVRSEADVARALAYARAAGLP
                     VSAAGVRHSMGGQAFRKGGVVLDMRGLDSIRLDPANRTITVGAGATWHAIQNAIHPHF
                     AVKAMQSTDIFTVGGSISVNAHGMDHRAGAMMGSIRSLRVMLADGTVVTASRDDNPDL
                     FRHVVGGYGLFGIVLSAELDVVPNRIYRSERSLVGYRDFPALFEAIAADPAVELSYTH
                     LSTAPGSLLEEALVYRYRRVGDETGLVRAPLGEVSSTRLRRLTVNLAKRADWLASLKW
                     WSEKNLEHRLEACTVTRAQAMRDGEACLVSRNDPMHDSVAYLANALPGETDILHEYFV
                     PRDRLVPFVDAMRTIFRGQDLKLLNASIRAVGTEENALSYAPRPAFSVVLYVNQPTTS
                     AGNAAMAGLTRSLIDLTIANGGRFFLPYQLHYTAEQLERSYPEIGAFFAAKKRWDPDG
                     RFRNSWYDRYAPDQ"
     gene            complement(2442..3512)
                     /locus_tag="VFU20_08350"
     CDS             complement(2442..3512)
                     /locus_tag="VFU20_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286499.1"
                     /translation="MKRRYRAALLVGGLLLAAVLAPVAYIEGACGRPDGAPAGAAARS
                     LLAPAERRPEARTWLTYPEWHIVYSAESFGSHLAAGKPPSDYPYGGDIAAFWRGACAV
                     NRVTAGLPGASDAKVMIYTIGISYSAELAIKAAWERTVGRFAQWAGGHDSADDRFAAR
                     VQQRYAAFMHETPWYRFPFGQALAGLWRTDEPRRPLRHWERRIALSLEYGVKAGYAKL
                     IGWASGATLGRDEPTLRFVARAAPGRLAAVDSRLKPVRAAPGGLTIVEAPRYAQFTDI
                     LRKLSAARVELVEIAGNDEIFFTALVEDGAGEPPAPLFAMPLGDRPGWRRVGLTVSVP
                     ALLPAIRVIETRGGRIEHVYDY"
     gene            complement(3509..3916)
                     /locus_tag="VFU20_08355"
     CDS             complement(3509..3916)
                     /locus_tag="VFU20_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4383 domain-containing protein"
                     /protein_id="HEU5286500.1"
                     /translation="MSNVQKMAWVYCAGFILVVLSGHYPPMLDSEGRQFGLFTLDLYD
                     HSLHLASGIWAGVAAWWSERAARLYFRIFGPLYFADGVLGLLTGSGFLDFGIFLYGPI
                     DQPMMTRFFANLPHLVIGGVAIWAGYRLAARER"
     gene            4103..4882
                     /locus_tag="VFU20_08360"
     CDS             4103..4882
                     /locus_tag="VFU20_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286501.1"
                     /translation="MTYHSLPALAALGLALAHPAAAETVLPTFDAGNFTGNPVVTSSY
                     FPVVEGQERTYLGSPADEQFVLTGVGSGPVILGIKTVTLRDRSFEGGLIVEDTFDYYA
                     QDKAGNVWYFGEDVTNYNYDRDGNLIGTDSASSWRAGVNGALPGYIMPVDQTVGFNYY
                     QEHAPEDDALDEGTTFAVLSSFTAGGVTYSNVLQVLETSDIDPKARGFKYYAPGVGLI
                     YEAEGLRPNNKNPRQIFELTEIGTASSASRPAFEFRRVPKN"
     gene            4965..5495
                     /locus_tag="VFU20_08365"
     CDS             4965..5495
                     /locus_tag="VFU20_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286502.1"
                     /translation="MAKLLEDRTRLLAIGLAAFALLLTLEWVTEDEDIDLADFLVDAI
                     ALALLVATSVAAALVAVDVREKHHDRLALEHDLERAKSEGAQWRKRAEDQLDQYRRAI
                     GEQFDEWDVTAAERDVGQLILRGLSHKEIARLRRTSEATVRQQAQSLYRKAHLPNKSA
                     FAAYFLDDVLYQAPAS"
     gene            5560..6588
                     /locus_tag="VFU20_08370"
     CDS             5560..6588
                     /locus_tag="VFU20_08370"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEU5286503.1"
                     /translation="MGYRVAVVGATGNVGREILNILAERQFPLAEVAAVASARSTGDV
                     VDFGEAGETLKVKNLDHFDFAGWDMALFAAGSETSKLHVPRAAAAGCTVIDNSSLYRM
                     DPDVPLIVPEVNPEAIAGYAKKNIIANPNCSTAQLVVALKPLHDAATIKRVVVATYQS
                     VSGAGKAGMDELFEQSRNIFVGDTSEPSHFTKQIAFNVIPHIDAFLDDGSTKEEWKMV
                     VETKKILDPAIKVTATCVRVPVFVGHSEAVNVEFEHELSAADARRILREAPGVMLVDK
                     REDGGYVTPVECVGDYATFVSRVRDDSTVEHGLSLWVVSDNLRKGAALNAVQIAELLG
                     RKHLQKAA"
     gene            complement(6585..7577)
                     /locus_tag="VFU20_08375"
     CDS             complement(6585..7577)
                     /locus_tag="VFU20_08375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286504.1"
                     /translation="MSSAPSAQEIARDARWLAQALDPDAGVVRLIEMDRQAYRAASFL
                     DDRILQAPVHAVTMPWSAVAAAIPAGARADARWIFHIGHVGSTLIARLLGELDGVLSV
                     REPRILRDLVAADRRERFLAQVRKLLSRTFAADEVALVKATSFVGEIAAELVPPGGRA
                     LFLFARPRAYIASILAGENSLKELDTLAGDRARRMAARVRGLGANTAAQLAAAAWACE
                     MTALEAAAEAMDDWQVLWADFDALLADMDAALVDLAGFFGFEAAPGRLREIAGGPLMG
                     RYSKALEHEYSPSLRRELIEQELRLQGRAIDDAVAMLERAATESPLLASALARR"
     gene            7606..8085
                     /locus_tag="VFU20_08380"
     CDS             7606..8085
                     /locus_tag="VFU20_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015456929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU5286505.1"
                     /translation="MHLHSAKEKRDMAGEAMEGGCACGRVRFRARIADDDAYLCHCRM
                     CQRATGSVSIAFKNLPQADVDWQSGPDWYDSSPIARRPYCRECGTSLGFQFKEGPNMD
                     LTVAAFDDPSRFRPTSHFGVESIHRTWLNTEGLPETRTDEHQKLVDKWIAATGKVPD"
     gene            8085..8837
                     /locus_tag="VFU20_08385"
     CDS             8085..8837
                     /locus_tag="VFU20_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003041965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5286506.1"
                     /translation="MTAAVHRWTASDGVELAWHEVGEGRAVILLHGLFSDAPMNWINF
                     GHADRIAAAGRRVIMPDLRAHGLSGKPHSPDAYPSAVLVRDLEELVARLGLAEFDLGG
                     FSLGARTVVQAVGEGLRPGKAVLAGMGLQGLRNWAARQAFFRDAIANFDTSARGDPHW
                     LAIQFMKTMKVDRVAAELVLGTFADLAPEWLDAFAMPTLVVCGREDEDNGSARELAEA
                     LPDATFAEVPGTHMSSVTKAELGEEIARFLTA"
     gene            8937..>10420
                     /locus_tag="VFU20_08390"
     CDS             8937..>10420
                     /locus_tag="VFU20_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009821616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008241 - peptidyl-dipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HEU5286507.1"
                     /translation="MKFAASVSLAALALAACAANPQLAAAPAAADQPAAVAAADPALT
                     AEGAREFVARVEKDLFDLSVIAGRAQWINATYITDDTDALAAHFGAIATEKQVLYAKE
                     AAKWIQAPGLDADTRRKLNILRTGLVLPAPSTPGAATELNEIATKLQSMYGKGRATHR
                     GKSITGDDAEELMGTLRNPAELTEVWKSWHENVGRPMRQDYARLVEIANAGAKELGFA
                     DTGAMWRSNYDMSPEEFSAMYERLWSEVKPLYNQLHCYTRTKLNQKYGNSVQPANGPI
                     RADLLGNMWAQEWGNIYDVVAPKGVGDVGYDLTDLLVRKKIDPTEMVRIGERFYTSLG
                     LAPLPQSFWQRSQIVRPEGREVVCHASAWDIDNKDDLRIKMCTKVNADDFVTIHHELG
                     HNYYQRAYKDQPYLYLNGANDGFHEAIGDFVALSVTPEYLVQIGLLDRSKVPDASKDT
                     GLLLRQAMDKVAFLPFGLLMDKWRWSVFSGAFKPEDYEGGWNALR"
BASE COUNT         1728 a         3654 c         3487 g         1551 t
ORIGIN      
        1 tcgaaggccg cccgctcgag ccggtctcgg ttggcgacta tgtcctctcg ggcggcgagc
       61 tcggtgcgat ggtgatcctc gacgcttgcg ttcgcctgct ccccggggta atgggcgccg
      121 cctccagcgg agaggaggag agcttcaacg aggggcttct cgaatatccg cattataccc
      181 ggcctgtgac atgggaaggg cgcacgatcc ccgaggtgct gcggtcgggg gatcatgcga
      241 ggatcgcggc gtggcgacac gaccgcgcat ccgaggacac acggctaagg cggccggacc
      301 tgatcgagcg ccacgggggc gcatcgcagg caccgccctc tggcgcgcgg cacgaagacg
      361 aaggcgacga ccaatgaacc tcatcgagac cctcgagcgc gaggccatcg acgctctcac
      421 cgaaatgcgc ccgatccccg aattccgccc cggcgacacg cttcgggtcg gggtcaaggt
      481 ggtcgagggc gaccgctcgc gggtgcagaa tttcgagggc gtgtgcatcg cccgctcgaa
      541 caagggcgtc ggctccagct tcaccgtccg caagatcagc ttcggcgagg gagtcgagcg
      601 cgtcttcccg ctctattcgc cggcgatcga ttcgatcgag gtcgtccgcc gcggcgcggt
      661 tcgccgggcc aagctctatt atctgcgcgg ccggaccggc aagtcggccc ggatcgccga
      721 gcgccgcgac gtccgcccgc ccaagggcag cgaggccgcc aagccggaga aggccgaggc
      781 ctgagccttc cgggcttgca tcggcgcgcg attgcgcgat gaaaaggcgc ccggagcgat
      841 ccgggcgtct ttttcgtggg gcgaggactt ggggggacga tgaaacgccg gactctgctg
      901 gtcggggtgt cgctgattgc cttggccgcg gccggcaccg ccgcctatgt cgcgccgctc
      961 gccgccgacc cggtccgagc caaggattgc ggcagcgccc tgccacgggc gacgtccctg
     1021 caggcgaggg cggccgccgt ccccgagccc gcctgggccc agcgcggcgg gacgatcaac
     1081 gacgccagct gcctgtcgcg caccccggtc gccggaatcg tcgcggtgcg gagcgaagcc
     1141 gatgtcgccc gggcgctggc ctatgcgcgc gccgccgggc tcccggtgtc cgcggccggg
     1201 gtcaggcatt cgatgggcgg ccaggcgttc cgcaaaggcg gcgtggtgct cgacatgcgc
     1261 ggcctcgatt cgatccgcct cgacccggcg aaccgcacca tcacggtcgg ggcgggcgcc
     1321 acctggcacg cgatccagaa tgccatccac ccgcatttcg cggtcaaggc gatgcagtcg
     1381 accgacattt tcaccgtcgg cggctcgata tcggtcaacg cgcacggcat ggaccatcgc
     1441 gccggcgcga tgatgggctc gatccgctcg ctccgcgtga tgctcgccga cggcacggtg
     1501 gtgaccgcct cgcgcgacga caaccccgac ctgttccggc acgtggtcgg cggctacggc
     1561 ctgttcggga tcgtcctgtc ggccgagctc gacgtcgtcc cgaaccgaat ctaccggtcg
     1621 gagcgctcgc tggtcggcta ccgcgatttc ccggcgctgt tcgaggcgat cgccgccgat
     1681 cccgccgtcg agctcagcta cacccatctg tcgacggcgc cgggctcgct gctcgaggaa
     1741 gccctggtct accgctatcg ccgggtcggg gacgagaccg ggctggttcg cgcgccgctc
     1801 ggcgaagtct cgtcgacccg gcttcgccgg ctgaccgtca acctcgccaa gcgcgccgac
     1861 tggctcgcgt cgctgaaatg gtggagcgag aagaacctcg agcaccggct ggaggcgtgc
     1921 accgtgaccc gggcgcaggc gatgcgggac ggcgaggcct gcctggtctc gcgcaacgac
     1981 ccgatgcacg acagcgtcgc ctatctggcc aatgcgctgc ccggggaaac cgacatcctc
     2041 cacgaatatt tcgtcccccg cgaccggctg gtgccgttcg tcgatgcgat gcggacgatc
     2101 ttccgcggcc aggacctcaa gcttttgaat gcgtcgatcc gggcggtcgg caccgaggag
     2161 aatgcgctga gctatgcgcc gcggccggcc ttctcggtcg tgctctatgt caaccagccg
     2221 accacgtccg cgggcaatgc cgcgatggcc gggctcacgc gctcgctgat cgacctcacc
     2281 atcgccaacg gcgggcgctt cttcctgccc taccaattgc attacacggc cgagcagctc
     2341 gagcgctcct atcccgagat cggggccttt ttcgccgcca agaagcgctg ggacccggac
     2401 gggcggttcc gcaacagctg gtacgaccgc tacgccccgg atcaataatc gtagacgtgc
     2461 tcgatccggc cgccgcgcgt ctcgatgacg cggatcgcgg gcaggagggc agggacgctc
     2521 accgtcagcc cgacccgccg ccagcccggc cggtcgccga gcggcatcgc gaacagcggc
     2581 gccgggggct cgccggcccc gtcctcgacc aaggcggtga agaaaatctc gtcgttgccg
     2641 gcgatctcga ccagctcgac cctggccgcg gacagcttgc gcaggatgtc cgtgaactgc
     2701 gcgtagcgcg gggcctcgac gatcgtcagc ccgccgggcg cggcgcggac cggcttcagg
     2761 cgcgaatcga ccgccgccag ccgcccgggc gcagcgcggg cgacgaagcg cagcgtcggc
     2821 tcgtcgcggc cgagcgtggc gccgctcgcc cagccgatca gcttcgcgta gccggccttg
     2881 accccatatt cgaggctgag cgcgatgcgc cgctcccaat ggcgcaatgg ccgccgcggc
     2941 tcatcggtcc gccacagccc ggccagcgcc tgtccgaacg ggaagcggta ccagggcgtt
     3001 tcgtgcatga aggctgcgta gcgctgctgg acccgggcgg cgaagcggtc gtcggcgctg
     3061 tcgtggccgc cggcccattg cgcgaagcgg ccgacggtgc gctcccacgc cgccttgatc
     3121 gccagctcgg ccgagtagct gatgccgatg gtgtagatca tcaccttggc gtcgctcgcc
     3181 ccgggcaggc cggcggtcac ccggttgacc gcgcaggccc cgcgccagaa ggcggcgatg
     3241 tcgccgccgt agggatagtc ggacggcggc ttgccggccg cgaggtgcga tccgaaactc
     3301 tcggccgaat agacgatgtg ccattcgggg taggtgagcc aggtccgcgc ctccggacgc
     3361 cgctccgccg gcgcgagcag cgaccgcgcc gcggctccgg ccggagcccc atcgggccgc
     3421 ccgcaggcgc cttcgatata ggccaccggc gccagcaccg ccgccagtaa cagcccgccg
     3481 accagcagcg cggcgcgata gcggcgtttc accgctcgcg cgcggcgagc cggtagccgg
     3541 cccagatcgc cacaccgccg atgaccaggt gcggcagatt ggcgaagaag cgggtcatca
     3601 tcggctggtc gatcggcccg tacaggaaga tcccgaagtc gaggaagccg ctgccggtca
     3661 ggaggccgag cactccgtcg gcgaaataga gcgggccgaa gatccggaag tagaggcgcg
     3721 ccgcgcgctc cgaccaccag gccgcgactc ccgcccagat acccgacgcc aggtgcaggc
     3781 tgtggtcgta gaggtcgagc gtgaacaggc cgaactggcg gccctccgaa tccagcatcg
     3841 gcggatagtg cccgctcagc accaccagga tgaacccggc gcaatagacc caggccattt
     3901 tctgaacatt gctcatcacg gcgctccccc catcggctcc cggcgcgact tatagccgcg
     3961 ctcgggcggg acgaaagcat cttccattta cccgggggcc cggcggcaac gtctacaccc
     4021 cgtccgccat gtgtcggatt gttcatcgtc ctgccgccgt aaataagccg ccgcgagcag
     4081 gggccgcgcc acggaggcga acatgactta ccattccctg ccggctttgg ccgcactggg
     4141 cctggccctt gcgcaccccg cagccgcgga gacggtgctg ccgaccttcg atgccgggaa
     4201 tttcaccggc aacccggtcg tcaccagctc ctatttcccg gtggtcgagg gacaggagcg
     4261 gacctacctc ggctcgccgg ccgacgagca gttcgtcctc accggggtcg gctccgggcc
     4321 cgtcatcctc ggcatcaaga cggtcacgct ccgcgaccgg tcgttcgaag gcggcctgat
     4381 cgtcgaggac acgttcgact attacgccca ggacaaggcc ggcaatgtct ggtacttcgg
     4441 agaggacgtc accaactaca attacgaccg cgacggcaat ctcatcggca ccgacagcgc
     4501 cagcagctgg cgggcgggcg tcaacggcgc gctgcccggc tacatcatgc ccgtcgacca
     4561 gaccgtcggc ttcaattact atcaggagca cgcgcccgaa gacgacgcgc tcgacgaggg
     4621 gacgaccttc gccgtcctgt ccagcttcac ggccggcggg gtcacttatt ccaacgttct
     4681 gcaggtgctg gaaacgtcgg acatcgaccc caaggcgcgc ggcttcaaat attacgcgcc
     4741 gggcgtcggc ctgatttacg aggccgaagg gctgaggccc aacaacaaga atccccgcca
     4801 gattttcgag ctgaccgaaa tcggcaccgc atccagcgct tcgcggccgg ccttcgagtt
     4861 ccggcgcgtg ccgaagaatt gatggcgcca tatttctggg ccgtgctgct ggctaactcg
     4921 ccgctgaccg gatagattcg tcgcggggaa gagcaggcgt tccaatggcc aagcttctcg
     4981 aggaccggac caggctgctg gcgatcggcc tagccgcctt cgcgctgctg ctgacgctcg
     5041 aatgggtcac cgaggacgag gacatcgacc tggccgattt cctggtcgac gcgatcgccc
     5101 tcgccttgct ggtggcgacg tcggtcgcgg cggcgctggt cgccgtcgac gtcagggaga
     5161 agcaccacga ccgcctcgcc ctggagcacg acctcgaacg ggcgaagtcc gagggcgccc
     5221 aatggcgcaa gcgcgccgag gaccagctcg atcaataccg ccgggcgatc ggcgagcagt
     5281 tcgacgaatg ggacgtcacc gccgccgaac gcgacgtcgg ccagctgatc ctgcgtggtt
     5341 tgagccacaa ggaaatcgca cggctgcgcc gcacttcgga agcgaccgtc cggcaacagg
     5401 cccaatcgct gtaccgaaag gctcatttgc ccaacaaaag cgcgttcgcc gcttacttcc
     5461 tcgacgatgt gctctatcag gcgccggcca gctgatgagc cgttgcgctt gagctgacgc
     5521 ttttgctagg cgagcggcgc cgtttcaaag gagtgttcaa tgggctaccg cgtggccgtc
     5581 gtgggcgcga ccggcaacgt cgggcgcgag atcctcaaca tcctcgccga gcggcaattc
     5641 ccgctggccg aggtggccgc ggtcgcctcg gcccgctcga caggcgacgt cgtcgacttc
     5701 ggcgaggcgg gcgagaccct gaaggtcaag aacctcgatc atttcgattt cgccggctgg
     5761 gacatggcgc tgttcgcggc cggctcggag accagcaagc tgcatgtccc gcgcgcggcc
     5821 gcggccggct gcacggtgat cgacaattca tcgctctacc ggatggaccc cgacgtgccg
     5881 ctgatcgtgc ccgaggtgaa ccccgaggcg atcgcgggct atgcaaagaa gaacatcatc
     5941 gccaacccca attgctcgac cgcgcagctg gtggtggcgc taaagccgct gcacgacgcg
     6001 gcgaccatca agcgggtggt cgtcgcgacc taccaatcgg tgtcgggcgc cgggaaggcc
     6061 ggcatggacg agctgttcga gcagtcgcgc aacatcttcg tcggcgacac gagcgagccg
     6121 tcgcacttca ccaagcagat cgccttcaac gtcatcccgc acatcgacgc gttcctcgac
     6181 gacggctcga ccaaggagga atggaagatg gtcgtcgaga ccaagaagat cctcgacccc
     6241 gcgatcaagg tcacggcgac ctgcgtccga gtgccggtgt tcgtcggcca tagtgaagcg
     6301 gtcaacgtcg agttcgagca cgagctttcc gccgccgacg cgcgccggat cctgcgcgag
     6361 gcgccgggcg tcatgctggt cgataagcgc gaggacggcg gctacgtgac gccggtcgag
     6421 tgcgtcggcg actatgcgac cttcgtttcc cgggtgcgcg acgattcgac cgtcgagcac
     6481 gggctgtcgc tgtgggtggt cagcgacaat ttgcgcaagg gcgcggccct gaatgctgtc
     6541 cagatcgccg aattgctcgg ccgcaagcat ctgcagaagg cggcctagcg ccgcgccagg
     6601 gcgctcgcca gcagcggcga ttccgtcgcg gcgcgctcga gcatcgccac cgcatcgtcg
     6661 atagcgcggc cctggagccg aagctcctgc tcgatcagct cgcgccggag cgacgggctg
     6721 tattcatgct cgagcgcctt ggagtagcgc cccatcagcg gcccgccggc gatctcgcgc
     6781 agccgcccgg gcgccgcctc gaagccgaag aatcccgcca aatccaccag cgccgcgtcc
     6841 atgtccgcga gcaacgcgtc gaagtccgcc cacaggacct gccaatcgtc catcgcttcg
     6901 gcggcggctt cgagcgcggt catttcgcac gcccaggccg cggcggcgag ctgcgcggcg
     6961 gtgttggcgc cgaggccgcg gacccgggcc gccatccgcc gcgcgcgatc gccggcgagc
     7021 gtatccagtt ccttcagcga gttctcgccg gcgaggatcg aggcgatgta ggcgcggggc
     7081 cgggcgaaca ggaacagcgc ccgaccgccc ggcggcacca gctcggcggc gatctcgccg
     7141 acgaagctgg tcgccttgac caatgcgacc tcgtcggcgg cgaaggtgcg cgacagcagc
     7201 ttgcgcacct gggcaaggaa gcgctcgcgc cgatccgcag ccaccaggtc gcgcaggatg
     7261 cgcggctcgc gcacgctgag caccccgtcg agctcgccga gcagccgcgc aatgagagtc
     7321 gagccgacat ggccgatgtg gaagatccag cgggcgtcgg cgcgcgcgcc ggcggggatt
     7381 gccgccgcga ccgcgctcca cggcatggta accgcatgga ccggcgcttg cagaatccgg
     7441 tcgtccagga agctcgccgc ccggtacgct tgccggtcca tctcgatcag ccgcacgacc
     7501 ccggcatcgg ggtcgagcgc ctgcgccagc cagcgcgcgt cgcgtgcgat ctcttgtgcc
     7561 gacggggcgc tggacatggc gccccttagc cgcgcaaagg gccgcgtgca tcttcattca
     7621 gccaaggaga agcgcgacat ggcgggcgag gcgatggaag gcggctgcgc gtgcggccgg
     7681 gtgcggttcc gggccaggat cgccgacgac gacgcctatc tgtgccactg ccggatgtgc
     7741 cagcgggcga ccggctccgt ctcgatcgcc ttcaagaacc tgccccaggc ggacgtcgac
     7801 tggcagtccg gccccgactg gtacgacagc tcgccgatcg cccgccggcc ttactgtcgt
     7861 gagtgcggca cctcgctcgg cttccagttc aaggaagggc cgaacatgga cctgaccgtc
     7921 gccgcgttcg acgacccgtc caggttccgc ccgaccagcc acttcggcgt agaatcgatt
     7981 caccgaacct ggctcaacac cgaaggcctg cccgagaccc gcaccgacga gcaccagaag
     8041 ctggtcgaca aatggatcgc ggccaccggc aaagtcccgg actgatgacg gccgctgtcc
     8101 accgctggac cgcgtccgat ggggtcgagc tcgcctggca cgaggtcggc gaggggcggg
     8161 cggtgatcct gctccacggc ttgttctccg acgctccgat gaactggatc aacttcggcc
     8221 atgccgaccg gatcgccgcc gcgggccgcc gggtgatcat gcccgacctt cgcgcccacg
     8281 gcctcagcgg caagccgcat tcgcccgacg catatccgag cgcggtcctg gtccgcgacc
     8341 tcgaggagct ggtcgcccgc ctcggcctcg ccgagttcga cctcggcggc ttttccctgg
     8401 gcgcccggac ggtcgtccag gcggtcgggg aggggctcag gcccggcaag gcggtgctcg
     8461 ccggaatggg cctccagggc ctgcggaact gggccgcgcg ccaggccttt ttccgcgacg
     8521 cgatcgccaa cttcgacacc agcgcgcgcg gcgacccgca ctggctggcg atccagttca
     8581 tgaagacgat gaaggtcgac cgagtcgccg cggagctcgt gctcgggacc ttcgccgacc
     8641 tggcccccga gtggctcgac gccttcgcca tgccgacgct ggtcgtgtgc ggccgcgagg
     8701 acgaggacaa cggctccgcg agggaactcg ccgaggcgct tcccgacgcg acgttcgccg
     8761 aagtcccggg cacccacatg agctcggtga ccaaggcgga actgggtgag gaaatcgccc
     8821 gttttctcac cgcctgacga ttggagtcga ccattcgccg aaccgggctg gcggattcct
     8881 gcgccgcttg gtgacgccgc cccgatgtga aacccgcctt gacgaggatg tccgtaatga
     8941 aattcgccgc ttccgtttcg ctcgccgcgc tggcgctcgc cgcctgcgcc gccaacccgc
     9001 agctcgccgc cgctccggca gcggccgacc agcccgcggc cgtcgccgcg gccgatccgg
     9061 cgctcacggc cgagggcgcg cgcgaattcg tcgccagggt cgaaaaggat ctgttcgacc
     9121 tgagcgtgat cgccggccgg gcgcagtgga tcaacgcgac ctacatcacc gacgacaccg
     9181 acgcgctcgc cgcccatttc ggcgccatcg ccaccgaaaa gcaggtcctc tacgcgaagg
     9241 aagcggcgaa gtggatccag gccccgggcc tcgacgccga caccaggcgc aagctcaaca
     9301 tcctgcgcac cgggctggtg cttccggccc cctcgacccc cggcgcggcg accgagctca
     9361 acgagatcgc gaccaagctg caatcgatgt acggcaaggg ccgggcgacc catcgcggca
     9421 agtccatcac cggcgacgat gccgaggagc tgatggggac gctgcgcaac ccggccgagc
     9481 tgaccgaggt gtggaaaagc tggcacgaga atgtcggccg gccgatgcgc caggactatg
     9541 cccggctggt cgagatcgcc aacgccggcg ccaaggagct gggctttgcc gacaccggcg
     9601 cgatgtggcg ctcgaactac gacatgagcc ccgaggaatt ctcggcgatg tacgagcggc
     9661 tgtggagcga ggtgaagcca ctctacaacc agctccactg ctacacccgc accaagctca
     9721 accagaaata cggcaattcg gtccagcccg ccaacggccc gatccgagcc gacctgctcg
     9781 gcaacatgtg ggcgcaggag tggggcaaca tctacgacgt cgtcgctccc aagggcgtcg
     9841 gcgacgtcgg ctacgatctg accgaccttt tggtgcgcaa gaagatcgac ccgaccgaga
     9901 tggtccgcat cggcgagcgc ttctacacgt cgctcggact cgccccgctg ccgcagagct
     9961 tctggcagcg ctcgcagatc gtccggccgg agggccgcga ggtcgtctgc cacgcgtcgg
    10021 cctgggacat cgacaacaag gacgatctgc gcatcaagat gtgcaccaag gtcaatgccg
    10081 acgacttcgt caccatccac cacgagctcg gccacaatta ttaccagcgc gcctacaagg
    10141 accagccgta cctgtacttg aacggcgcca acgacggctt ccacgaggca atcggcgatt
    10201 tcgtcgcttt gtcggtgacg cccgaatatc tggtccagat cggcctgctc gaccgctcca
    10261 aggtgcccga tgccagcaag gacaccggcc tgctgctgcg ccaggcaatg gacaaggtcg
    10321 cgttcctgcc gttcggactg ttgatggata aatggcgctg gagcgtcttc tccggcgcgt
    10381 tcaagcccga ggattatgaa ggcggctgga acgcccttcg
//
LOCUS       DASTZO010000298         2301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_259801,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000298 DASTZO010000000
VERSION     DASTZO010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_259801"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="VFU20_08395"
     CDS             complement(<1..1373)
                     /locus_tag="VFU20_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HEU5286508.1"
                     /translation="MTSRKITRRARLGTAFAALAIGLGTIAGSAPALAQQSAARPSET
                     LNLSQGTGTLVRLSAPMSDVFVANDTIADVQVRSATQLYVFGKGRGETTVYATGKNGK
                     VVYAANVRVGNNISSVGEMLSLAMPEASIQATPMNNLVLLTGTVSSPTDVEEAQRLVQ
                     AYVGEGTQVVSRLRSAVPLQVNLKVKIAEVNRSLLKQIGVNLFSSDPTSGFQFGIGQG
                     TPGAVSGGGGSPFSVGSLLTGGTTVGAAGRLFGLDIAGAIDLAEKDGLVSILAEPNLT
                     ALSGETASFLAGGEFPIPISQSLGAVTIEYKQYGVGLAFTPIVLGDGRISMRVRPEVS
                     ELSNEGAVKLNDFLVPALTTRRAETTVELGSGQSFMIAGLLRNSNTNDINKAPFLGDL
                     PILGALFRSTSFRRSETELVIIVTPYLVRPVSGQLAMPTNGYRAPSDVQRVLEGQTFV
                     GPGAPRGG"
     gene            complement(1375..>2301)
                     /gene="cpaB"
                     /locus_tag="VFU20_08400"
     CDS             complement(1375..>2301)
                     /gene="cpaB"
                     /locus_tag="VFU20_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013833117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HEU5286509.1"
                     /translation="GPKVLVARKALPVGTIIDAESLAYQPWPKELIQSAYYTEGSPDS
                     DMTKLLGTVVRYPVTAGQPLTHGALVGPNDRGFLAAALGPGMRAVTVPVNVATGVAGF
                     VFPGDRVDIVLTQAVEGGGDGPPLKVSETIVRNVRVLATDQRIDSKGDDGKTEVKTFS
                     NVTLESTPRIAEKIAVAQSLGTLSLSLRSIADNTAELERLVASGEVRLPQGANPAEER
                     RLLLAVANRPVDTNTTYTTGGDVSRFQRRSVPGRASTSGSGPNDALASFAKALSGGPA
                     TSGTASAAPVYSGPVVRISRGNTVTVVPVGAR"
BASE COUNT          344 a          749 c          846 g          362 t
ORIGIN      
        1 cctccgcgcg gggcacccgg gccgacgaag gtctggccct cgagcacccg ctggacgtcg
       61 ctcggagcgc ggtagccgtt ggtcggcatc gccagctggc cggacaccgg ccgcaccaga
      121 tagggcgtga cgatgatcac cagctcggtc tccgaccggc ggaagctggt cgagcggaac
      181 aaggcgccga ggatcggcag gtcgccgagg aacggcgcct tgttgatgtc gttggtgttc
      241 gagttgcgaa gcaggccggc gatcatgaac gactggcccg agccgagctc gacggtggtc
      301 tcggcgcggc gcgtggtcag cgccgggacg aggaagtcat tcaatttgac cgcgccctcg
      361 ttgctgagct cgctgacctc gggtcggacg cgcatcgaga tgcggccgtc gccaaggacg
      421 atcggagtga aggccaggcc gacgccatat tgcttatact cgatggtcac cgctcccagc
      481 gactgggaaa tcgggatcgg gaactcgccg ccggcgagga agctggccgt ctcgcccgac
      541 aaagcggtga ggttcggctc ggcgaggatg ctgaccaggc cgtctttctc ggcgaggtcg
      601 atcgcaccgg cgatgtcgag gccgaacaag cggccggccg cgccgacggt cgttccgccg
      661 gtcagcagcg agccgaccga gaagggcgag ccgccgccgc cgctgaccgc gcccggcgtg
      721 ccctggccga ttccgaactg gaagccgctg gtcgggtcgc tcgagaagag gttgaccccg
      781 atctgcttca gcaggctgcg gttgacctcg gcgatcttga ccttgaggtt gacctgcagc
      841 ggcaccgccg agcggagccg gctgacgacc tgcgtgcctt cgccgacata ggcctggacc
      901 aggcgctgcg cctcctcgac gtcggtcggg gacgacaccg tgccggtcag gagcacgagg
      961 ttgttcatcg gggtcgcctg gatgctcgcc tcgggcatcg ccaggctgag catctcgccg
     1021 accgagctga tattgttgcc gacgcggacg ttggccgcat agacgacctt gccgttcttg
     1081 ccggtcgcgt agaccgtggt ctcgccgcgc cccttgccga acacgtagag ctgggtcgcc
     1141 gagcgcacct ggacgtcggc gatcgtgtcg ttggcgacga acacgtcgct catcggcgcg
     1201 gaaagccgga ccagagtgcc ggtcccctgg ctcaggttga gcgtctccga gggccgcgca
     1261 gcgctttgct gggcgagcgc gggggcggat cccgcaatcg ttccgaggcc gatggccagg
     1321 gcggcgaatg cggtgccgag acgcgcccgg cgagtgattt tacgactggt catgttagcg
     1381 tgctcccacc ggaacgacgg tgacggtatt gccgcggctg atgcgcacga ccggaccgga
     1441 atagaccgga gcggcacttg ccgttccact ggtcgcgggt ccgccgctca acgccttggc
     1501 aaaggatgcg agtgcgtcgt tcgggcccga gcccgacgtc gacgcccggc cggggacgct
     1561 gcggcgctgg aagcgcgaga catcgccacc ggtcgtgtac gtggtgttgg tgtcgaccgg
     1621 gcggttggcg accgccagca gcaggcgccg ctcctcggcc ggattggcgc cctgcggcag
     1681 ccggacctcg cccgaggcga cgagccgctc gagctcggcg gtgttgtcgg cgatcgagcg
     1741 cagcgacagc gacagcgtgc ccaggctctg cgcgacggcg atcttctcgg cgatccgcgg
     1801 ggtcgattcg agcgtgacgt tcgagaaggt cttgacctcg gtcttgccgt cgtcgccctt
     1861 gctgtcgatg cgctggtcgg tggcgagcac gcgcacgttg cgcacgatcg tctcggaaac
     1921 cttcaacggc gggccgtcgc cgccgccctc gaccgcctgg gtcaggacga tgtcgacccg
     1981 gtcgcccggg aagacgaagc cggcgacccc ggtcgcgacg ttgaccggga ctgtgacggc
     2041 gcgcatcccc gggccgagcg cagcggcgag gaagccgcgg tcgttcggtc cgacgagggc
     2101 gccgtgggtc agcggctggc cggcggtgac cggatagcgg accacggtgc cgagcagctt
     2161 ggtcatgtcc gaatcgggcg agccctcggt gtagtaagcg ctctggatga gctccttggg
     2221 ccacggctga taggcgaggc tctcggcatc gatgatcgtg ccgaccggaa gcgccttgcg
     2281 ggcaaccagg accttgggcc c
//
LOCUS       DASTZO010000299         4361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1147801,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000299 DASTZO010000000
VERSION     DASTZO010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147801"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..1647)
                     /locus_tag="VFU20_08405"
     CDS             complement(172..1647)
                     /locus_tag="VFU20_08405"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEU5286510.1"
                     /translation="MSTDDRAVGEPERLHPLFLLTGLGRSLRGMAGGYAFIGYLAVSG
                     RASTALIAAVVLLAFLAASLFVYWTRFEFRVGSSEIRVDSGIFSRTHRSIPFDRIQDV
                     DISQGPVARLLDIAKVKFETGGSAGAGEDEGVLHAIPLERAQELRALVRSRRSAADEP
                     VPTTAEAERPPVFAMDLPRVLLAGVFNFSLALFAGLIGATQTFGEMLGFDPLSRSFWL
                     GLLRAGDPIADYILAHRVAAAFAGAVLLVLIGLATGIVRTLLREYGFRLDRTEVGLRR
                     RRGLLTRTDVTLPARRAQAAIVGTGPLRDSLGWRDLKLQSLASDDGGGGDHVVAPLAT
                     DEEAGAILAELGWRPLSGTTDWKRVSSVYVWTLAIGLSPLLLAAALASTQAPNSAVAI
                     AVLLALLVIRWRGWRRTLYALDGDRLLIRSGWWRRRTVILPLARIQSIDLTESFVSRW
                     FGIAGLAFGVAGGSGFSEHSIPALPRETARALRKRLLVMEP"
     gene            complement(1658..2158)
                     /locus_tag="VFU20_08410"
     CDS             complement(1658..2158)
                     /locus_tag="VFU20_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HEU5286511.1"
                     /translation="MDGVSEGGIAAETLLQPVEPGYRHVLRLRLLLLWLPLAIAALVF
                     DNGMVAGTPFYGGLSILVPLAATLVVAIAPQRIYRRLRYGLTERLLQVVRGWLFHTDT
                     IVPFVRVQHLDVTRGPLDKLFGTATLVVHTAGTRNSIVTVPGLAPGRAAEIRDVIREH
                     IRTDFE"
     gene            complement(2146..3132)
                     /gene="glpX"
                     /locus_tag="VFU20_08415"
     CDS             complement(2146..3132)
                     /gene="glpX"
                     /locus_tag="VFU20_08415"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HEU5286512.1"
                     /translation="MNSAGPAASTVLDRVLVLEMVRVTEAAAIAASKLIGRGDEKAAD
                     HAAVEAMRAALNELPMDGTVVIGEGERDEAPMLYIGEKVGSAQGTGPRIDIALDPLEG
                     TTITAKAGPNALAVLAIAEQGCLLNAPDVYMDKLAIGPGYPQGTIDLARSASDNIRSI
                     AAAKGVDPSEIIVCVLDRPRHAELIAELRSLGCGVRLIPDGDVAGVIDTTDPDTGIDV
                     YMGSGGAPEGVLAAAALRCVGGQIQGRLLFRNDDEIARARRWGIEDLDRIYALEDMAK
                     GDCIFAATGVTDGSLLDGVHRRKTCITTESIVMRASSGTVRRVTAEHHRWTE"
     gene            3238..4242
                     /locus_tag="VFU20_08420"
     CDS             3238..4242
                     /locus_tag="VFU20_08420"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5286513.1"
                     /translation="MMRIGIAAALLALGTPAAAAVPPPVIDMHLHATSADSAGPPPQG
                     MCTPFNEFPVWDQRRSYADIFMDRFKKPRCADPVWSPTTDDALMRETIAAMERRNVYG
                     VLSGSAEKVAAWRKAAPGRFWSGLAFSLAGKISPQRVEQLHRAGAVDVFGEWSTQYEG
                     IEPADPRLEPYWALAEKLDIPVGIHVGTGPFGVIYMGSTGYRARMHSALTMEEVLVRH
                     PKLRVYLMHAGYPMIDDLLALLYAHPQVHVDTGVIIFTQPRAAFYRYFQRITEAGFAN
                     RVMFGSDQMVWPGVIERSIKVIEDAPFLTESQKRDILYNNAARFLRLSDQEIARHHGR
                     "
BASE COUNT          691 a         1498 c         1555 g          617 t
ORIGIN      
        1 ggaggaagtc gcggatggtc gggctgcggt ggcgggcgag gtcggaccgc tcgaccccgc
       61 gggccagcag cagctcgtcg accagcgcgt ccggggccag cccgggctcg cccgagcgcc
      121 gccagcgcca cggctggccg ccgaccgacc gctcgacatt caaggcgaag ctcatggctc
      181 catcactagc agccgcttgc gcagcgcgcg cgcggtttcg cgcggcaagg cggggatcga
      241 atgctcggaa aagccgctcc cgccggcgac cccgaaggcc agcccggcga tcccgaacca
      301 gcggctgacg aagctctcgg tgaggtcgat gctctggatc ctggccagcg gcaggatgac
      361 ggtccgccgc cgccaccagc cggagcggat cagcagccgg tcgccgtcaa gcgcgtagag
      421 cgtccggcgc cagccgcgcc agcggatcac caggagcgcg agcaggacgg cgatcgcgac
      481 cgcggagttc ggcgcctggg tcgaggcgag ggccgcggcc agcagcagcg gcgacaggcc
      541 gatcgccaga gtccagacgt agaccgacga aacccgcttc cagtcggtcg tccccgacag
      601 cggccgccag ccgagctcgg cgaggatcgc gccggcctct tcgtccgtgg ccagcggggc
      661 cacgacatgg tcgccgccgc ccccgtcgtc gctggcgagg ctctgcagct tgaggtcgcg
      721 ccagccgagc gaatcacgca acgggccggt gccgacgatc gccgcctgcg cgcgccgcgc
      781 cgggagggtg acgtcggtcc gggtcagcag gccgcggcgg cggcgaagcc cgacctcggt
      841 gcggtcgagc cggaagccgt attcgcgaag cagggtccgg acgatccccg tcgccagccc
      901 gatcagcacc agcagcaccg ctccggcgaa tgcggcggcg acccggtggg cgaggatata
      961 gtcggcgatc gggtcgccgg ccctcagcag cccgagccag aatgagcggc tgagcgggtc
     1021 gaagccgagc atctcgccga acgtctgggt cgcgccgatc agcccggcga acaaagccag
     1081 cgagaaattg aacacccccg cgagcagcac tcggggcagg tccatcgcga acaccggcgg
     1141 ccgctcggcc tcggcggtcg tcggcaccgg ctcgtccgcg gccgagcggc gcgagcggac
     1201 cagcgcccgt agctcctgcg cccgctcgag cgggatcgcg tgaagcacgc cctcgtcctc
     1261 gccggccccg gccgagccgc cggtctcgaa cttgaccttg gcgatgtcga gcagccgggc
     1321 gaccggcccc tggctgatgt cgacgtcctg gatgcggtcg aacgggatcg accggtgggt
     1381 ccggctgaag atgccgctgt cgacccggat ctcgctcgac ccgacccgga actcgaagcg
     1441 ggtccagtag acgaacaggc tggccgcgag gaaggcgagc agcaccaccg ccgcgatcag
     1501 cgccgtgctc gcccgcccgg agaccgcgag atagccgatg aacgcatagc cgccggccat
     1561 cccgcgcagg cttcggccga ggccggtcag aaggaacaag ggatggagcc gctcgggctc
     1621 gccgaccgcc cggtcgtccg tactcatggc cggacgctca ttcgaaatcc gtgcggatat
     1681 gctcgcggat gacgtcgcgg atctccgcgg cgcggcccgg ggccaggccc ggcacggtga
     1741 cgatgctgtt gcgggtgccg gcggtgtgga cgaccagggt cgcggtgccg aacagcttgt
     1801 cgagcggccc gcgggtgacg tcgaggtgct ggacccggac gaacgggacg atggtgtcgg
     1861 tgtggaacag ccagccgcgc accacctgca gcagccgctc ggtgaggccg tagcgcaggc
     1921 gccggtagat gcgctgcggg gcgatcgcga cgacgagggt cgcggccagc ggcacgagga
     1981 tgctcaagcc cccgtagaat ggcgtgccgg caaccatccc gttgtcgaaa accagggcgg
     2041 cgatcgccag cggcagccac aggagaagaa gccgaaggcg cagcacgtgg cggtagccgg
     2101 gctcgacggg ctgcagcagc gtctcggcag cgatgccgcc ctcgctcact ccgtccatcg
     2161 atggtgctcg gcggtcaccc ggcgcacggt gcccgagctg gcccgcatga cgatgctctc
     2221 ggtggtgatg caggtcttgc ggcggtggac gccgtcgagc agcgagccgt cggtgacccc
     2281 ggtcgcggcg aagatgcagt cgcccttggc catgtcctcg agcgcgtaga ttcggtccag
     2341 gtcctcgatc ccccagcggc gggcgcgggc aatttcgtcg tcgttgcgga acagcagccg
     2401 gccctggatc tgcccgccga cgcagcgcag cgcggccgcg gcgagcacgc cctcgggcgc
     2461 cccgccggag cccatgtaga cgtcgatccc ggtgtcgggg tcggtggtgt cgatcactcc
     2521 ggcgacgtcg ccgtcgggga tcagccggac tccgcagccg aggctccgca attcggcgat
     2581 cagctcggcg tggcgcggcc ggtcgagcac gcagacgatg atctccgacg ggtcgacgcc
     2641 cttggccgcg gcgatcgagc ggatattgtc cgaggcgctt cgggcgaggt cgatggtgcc
     2701 ttgcggatag ccggggccga tcgccaattt gtccatgtag acgtcgggcg cattgagcaa
     2761 acatccctgt tcggcgatcg ccagcacggc gagcgcgttc gggcccgcct tggcggtgat
     2821 ggtggtgcct tccagtgggt cgagcgcgat gtcgatcctc gggccggtcc cctgggcgct
     2881 gccgaccttt tcgccgatgt acagcatcgg cgcctcgtcg cgctcgccct cgccgatcac
     2941 caccgtcccg tccatcggca gctcgttcaa ggcggcgcgc atcgcctcga cggccgcatg
     3001 gtcggcggcc ttctcgtcgc cgcgcccgat cagcttggag gcggcgatcg ccgcggcctc
     3061 ggtgacccgc accatctcga gcaccaggac tcggtcgagg acggtcgaag cggcggggcc
     3121 ggcactgttc atgggctcaa gaaactcctc gacgggggat gctttcggat agtcacgccg
     3181 caacggcttg tcgaggcggc gccgataccg tctagtaggt gacgattagg gagacaaatg
     3241 atgcgcatcg ggatcgcggc ggcgctgctc gcgcttggaa cgccggcagc ggcggcggtg
     3301 cccccgccgg tgatcgacat gcacctgcat gcgaccagcg ccgactccgc gggcccgcca
     3361 ccgcagggca tgtgcactcc gttcaacgag tttccggtgt gggaccagcg tcgatcctat
     3421 gccgacatct tcatggaccg gttcaagaag ccgcgctgcg ccgacccggt gtggtcgccg
     3481 acgaccgacg acgcgctgat gcgcgaaacc atcgccgcca tggagcggcg caacgtctat
     3541 ggcgtccttt ccggcagtgc cgaaaaggtc gcggcatggc gaaaggcggc cccgggccgc
     3601 ttctggtccg gcctcgcgtt cagcctggcg ggcaagatca gtccacagcg cgtcgagcag
     3661 ctccaccggg ccggagcagt cgatgtgttt ggcgaatgga gcactcaata tgaagggatc
     3721 gagccggccg acccgcggct cgagccctat tgggcgctcg cggagaagct cgacatcccg
     3781 gtcggcattc acgtcggtac gggcccgttc ggagtgatct acatggggtc gacgggctac
     3841 cgcgcgagaa tgcacagcgc gctgaccatg gaggaggtgc tggtccggca cccgaagctg
     3901 cgcgtctatc tgatgcatgc cggctatccg atgatcgatg atctgctggc cctgctctac
     3961 gcgcacccgc aggttcatgt ggacaccggt gtgatcatct tcacccagcc gcgggccgct
     4021 ttctatcgct acttccagcg gatcaccgaa gccggcttcg ccaatcgcgt gatgttcggc
     4081 tccgaccaga tggtctggcc gggcgtgatc gagcgctcga tcaaggtaat cgaggacgcg
     4141 ccgttcctga ccgagagcca gaagcgcgac atcctctaca acaatgccgc gcgcttcctg
     4201 cgcctgagcg accaggagat cgcccgccac cacgggcgct agatcagtct tcgtcggcct
     4261 ggtgctcgag cagttcggtg cggatgcgca ccgagcgcga gccgatccgg gtctcgacca
     4321 ggaagatcgc gaagccggca gcgatcgcga tcatcgagcc g
//
LOCUS       DASTZO010000300         5438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_181283,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000300 DASTZO010000000
VERSION     DASTZO010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_181283"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..900)
                     /locus_tag="VFU20_08425"
     CDS             complement(109..900)
                     /locus_tag="VFU20_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEU5286514.1"
                     /translation="MSKITRLAAAFTACALVLPAAGCAGNKGGAGDTAYVARDVNTLY
                     ALAKRKMDQGLYEESAKLFDEVERQHPYSVWARRAQLMSAFSYYLARKYPDAISSARR
                     FLTIHPGNKEAPYAHYLVAMSHYQQMEDVTRDQRITQQAADAFGELVRRYPESRYAAD
                     ARVKLDLINDHLAGKEMEIGRFYQRSGNWLAATNRFRTVIDKYQTTSHTPEALHRLVE
                     SYLALGIPVEARKAAVVLGANYPDSVWYHRAYRLIYYKEPKGIRG"
     gene            complement(1141..2091)
                     /gene="secF"
                     /locus_tag="VFU20_08430"
     CDS             complement(1141..2091)
                     /gene="secF"
                     /locus_tag="VFU20_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU5286515.1"
                     /translation="MRLLKLVPDTTNIDFMGWRNVALVISILVTLASLALVGVRGLNL
                     GVDFVGGQMIRTTFAQPIDVDQLRSRVGALDLGDASIQESGGPRTYQIRLPRPEGGEA
                     AANRAASQVRSMIERSYPGARVDAVETVSGKVSEELALDGALAITLAMLGIAIYIWFR
                     FEWQFGVGALVTLFHDVSLIFGFFALTQLQVDLNIVAAFLAIVGYSLNDTVVIYDRIR
                     ENLRKYRKMGIIPLLNLSLNETLSRTIVTALSIMLALAVLLVIGPDVIFGLTLAILLG
                     TFIGTYSSIYISAPMLTWLGLKPDSFLQVDEKNLPEPQEA"
     gene            complement(2102..3697)
                     /gene="secD"
                     /locus_tag="VFU20_08435"
     CDS             complement(2102..3697)
                     /gene="secD"
                     /locus_tag="VFU20_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU5286516.1"
                     /translation="MLDFPRWKIWAVSLTVAIGILFAIPSLIAGTPLADRYPGWLPSA
                     RISLGLDLSGGSQLLLEADAADAAKQRLAAMEDSVSTELRRARPRIEIGDISTTGGRL
                     SFLVRDPTRVDAAVERLRTLTQPVALTGARDWDVSVVDSTRIVLTPTRSGSAQALRDA
                     MTVARDVVRRRIDPQGTKEVTVINQGERRILVQVPGVEDPEALKKLIGQTARLEFKLV
                     DLTADPAQVAQGRAPAGSQVLPLADGTGAMAVKRRVMVSGDQLVDAKQTFDQDGQPVV
                     SITFNSAGARRFGRVTQENVNKPFAIILDEKILSAPNINEPILGGQAQISGNFTVESA
                     NALAISLASGKLPVKLNVIEERTVGPELGKDSIRKGTVASIVATLAVIAFMLVTYGRF
                     GVYATLGLLVNALMILGIMAIFGAALTLPGIAGFVLTIGAAVDANVLINERIREEQRR
                     GRKILDSIETGYKEASTAIFDANITNVIAAALMWYFGSGPIRGFAVVLMIGIVTSVFT
                     AVNFTRMLVALWIRRKRPRALYI"
     gene            complement(3697..4023)
                     /gene="yajC"
                     /locus_tag="VFU20_08440"
     CDS             complement(3697..4023)
                     /gene="yajC"
                     /locus_tag="VFU20_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU5286517.1"
                     /translation="MTTPLLMTATPASDPSAIFVGILPWLLIFVIFYLLMIRPQQKRM
                     KEHAATIAAVKKGDEVITGGGIRGRVTKVMDDDVEVEIGQNVRVRIVKATLSHVVTKS
                     AKPAND"
     gene            complement(4054..4130)
                     /locus_tag="VFU20_08445"
     tRNA            complement(4054..4130)
                     /locus_tag="VFU20_08445"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4094..4096),aa:Pro,seq:ggg)
     gene            complement(4266..>5438)
                     /locus_tag="VFU20_08450"
     CDS             complement(4266..>5438)
                     /locus_tag="VFU20_08450"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HEU5286518.1"
                     /translation="VNDTLGHPIGDELLKQAAVRLRDIAGKDEFVSRLAGDEFAVIQR
                     RIKQPRQAELLADRIIAGLSNPFEIDGHTIAIGASVGIALAPKDSRDGAELLKKSDLA
                     LYWAKNVGRGGFCFYKPRMDKHLNERRALGNDLRAAVQNGQFELYYQPLLNLSSRRIC
                     GMEALVRWHHPRRGMLQPSDFIPVAEESGLIVPIGEWVLRQACIEAANWPKPISVAVN
                     LSAAQFKRGDLLAMTTAALKAAGLESGRLELEITESVLLNDEIWVRNVLCKLRDLGVR
                     LAMDDFGTGYSSLSYLRSFPFSKIKIDRSFVSDITVTRDALAIVQATIQLSEKLGIGT
                     TAEGIETAGQLHLLAAEGCTEAQGFHISRPVRAQEIPALISRYGCHVERPALRVAS"
BASE COUNT          897 a         1723 c         1899 g          919 t
ORIGIN      
        1 gagccggtcg accagcacca cgtcgcggat ggcgagctgc cttagcatcg ccgcttgtct
       61 agtggatcgg cgggcggcgg ccaatcgccg ccgtgcgcac cggaagactc agccccggat
      121 gcccttcggc tctttgtaat aaatcagccg gtaggcccgg tggtaccaga cgctgtcggg
      181 ataattggcg ccgagcacga ccgcggcctt gcgcgcctcg accgggatgc cgagcgcgag
      241 ataggattcg accagccggt ggagcgcctc gggcgtgtgg ctggtggtct ggtatttgtc
      301 gatgacggtg cggaagcggt tggtcgccgc gagccagttg cccgagcgct ggtagaagcg
      361 cccgatctcc atctccttgc cggcgagatg gtcgttgatg aggtcgagct tgacccgcgc
      421 gtcggccgcg tagcggcttt cgggatagcg ccgcaccagc tcgccgaacg cgtcggccgc
      481 ctgctgggtg atgcgctggt cgcgggtcac gtcctccatc tgctggtagt ggctcatcgc
      541 caccagataa tgggcgtagg gcgcttcctt gttacccggg tggatggtca ggaagcggcg
      601 cgccgagctg atcgcgtcgg gatatttgcg cgccagatag taactgaagg cgctcatcag
      661 ctgggcgcgg cgggcccaca ccgaataggg gtgctggcgc tcgacctcgt cgaacagctt
      721 ggccgattcc tcgtacaggc cctggtccat cttgcgcttg gcgagcgcgt agagcgtgtt
      781 gacgtcgcgg gcgacgtagg cggtgtcgcc ggcgccgccc ttgttgccgg cgcaaccggc
      841 ggcgggaagg acgagcgcgc aggcggtgaa cgccgcggcg agacgggtga ttttggacat
      901 ggcgacctct tagcgcggcc cccggcgaag gccaagccga gagatggcgg gcgaatgatg
      961 aacgcgggat ttctgcgtga ccggcgaatt cgtggaaggg agaccagcac agctggtcga
     1021 ggcgttcgac cgccgcgccc gagcggcacc tgctgtccac cttgcgccga aagcaggcat
     1081 tcgacggtca tgggatgcgc gggttcggca tcccgaagtc ggtatcgccg cgcctctcgc
     1141 tcaggcttcc tggggctcgg ggaggttctt ttcgtcgacc tggaggaagc tgtcgggctt
     1201 caatcccagc caggtcagca tcggcgccga gatgtagatc gacgaatagg tgccgatgaa
     1261 ggtgccgagc aggatcgcca gggtcagccc gaagatcacg tcggggccga tgaccagcag
     1321 caccgccagg gcgagcatga tcgacagagc ggtgacgatg gtccgcgaca gggtctcgtt
     1381 cagcgacagg ttcaagagcg ggatgatgcc catcttgcgg tacttgcgca ggttctcgcg
     1441 gattcggtcg tagatcacca ccgtgtcgtt gagcgagtaa ccgacgatcg cgaggaaggc
     1501 ggcgacgatg ttcaagtcga cctgcagctg ggttagcgcg aaaaagccga agatcaggct
     1561 gacgtcgtgg aacaaagtga ccagcgcgcc gaccccgaac tgccattcga agcggaacca
     1621 gatgtagatg gcgatcccga gcatcgccag ggtgatcgcc agcgcgccgt cgagcgcgag
     1681 ctcctcgctg accttgcccg acacggtctc gaccgcgtcg acccgggcgc ccggatagct
     1741 gcgctcgatc atcgagcgga cctgcgaggc ggcgcggttg gcggccgcct cgccgccttc
     1801 gggccggggc agccggatct ggtaggtcct cggtccgccc gattcctgga tgctcgcgtc
     1861 gccaagatcg agggcgccga cccggcttcg cagctggtcg acgtcgatcg gctgcgcgaa
     1921 ggtcgtgcgg atcatctgcc cgccgacgaa atcgaccccg aggttgagcc cgcgcacccc
     1981 gaccagcgcc aggctggcga gggtgacgag gatcgagatg accagcgcga cgttgcgcca
     2041 gcccatgaag tcgatgttgg tcgtgtcggg gacgagcttc aggagccgca ttgaatcagc
     2101 ctcatatgta gagagcgcgc ggccgcttgc ggcggatcca cagggccacc agcatccggg
     2161 tgaaattgac cgcggtgaac accgaggtga cgatgccgat catcagcacc acggcgaagc
     2221 cgcggatcgg cccggagccg aaataccaca tcagggccgc ggcgatgacg ttggtgatgt
     2281 tggcgtcgaa gatcgccgtc gaggcttcct tgtagccggt ctcgatcgaa tcgaggatct
     2341 tgcggccgcg cctctgttcc tcgcggatgc gctcgttgat cagcacgttg gcgtcgaccg
     2401 cggcgccgat ggtcagcacg aagccggcga ttccgggaag agtcagggcg gccccgaaga
     2461 tcgccatgat gccgaggatc atcagcgcgt tcaccagcag gccgagcgtc gcatagaccc
     2521 cgaagcggcc ataggtgacg agcatgaagg cgatcaccgc gagggtcgcg acgatgctgg
     2581 cgacggtgcc cttgcggatc gaatccttgc cgagctcggg gccgacggtg cgctcctcga
     2641 tcacgttcaa tttcaccggc agcttgcccg aggcgaggct gatcgcgagg gcgttggcgc
     2701 tttcgacggt gaagttgccg ctgatctgcg cctggccgcc gaggatcggc tcgttgatgt
     2761 tcggcgccga caggatcttc tcgtcgagga tgatcgcgaa cggcttgttg acgttctcct
     2821 gggtgacccg gccgaagcgc cgcgcgccgg cgctgttgaa ggtgatcgag accaccggct
     2881 ggccgtcctg gtcgaaggtc tgcttggcgt cgaccagctg gtcgcccgag accatcaccc
     2941 gccgcttgac cgccatcgcg ccggtgccgt cggcgagcgg cagcacctgg ctcccggccg
     3001 gggcgcggcc ctgagcgacc tgcgccgggt cggcggtcaa atcgaccagc ttgaactcga
     3061 gccgggcggt ctggccgatc agcttcttga gcgcttcggg gtcctcgacc ccgggcacct
     3121 ggacgaggat gcgacgctcg ccctggttga tgaccgtgac ctccttggtg ccctgggggt
     3181 cgatcctgcg gcggacgacg tcgcgggcga cggtcatcgc gtcgcgaagc gcctgcgccg
     3241 agccggagcg ggtcggggtg aggacgatgc gggtcgaatc gacgacgctg acgtcccagt
     3301 cgcgggcccc ggtgagcgcg accggctggg tcagggtgcg cagccgctcg accgcggcgt
     3361 ctacccgggt cgggtcgcgg accaggaacg acaggcgccc gccggtggtc gagatgtcgc
     3421 cgatctcgat ccgcggcctg gctcggcgga gctcggtcga gaccgaatcc tccatcgccg
     3481 cgagccgctg cttggcggcg tcggcggcgt cggcctcgag cagcaactgg ctgccgcccg
     3541 acaggtcgag gccgaggctg atccgggcgc tcgggagcca gccgggatag cggtcggcca
     3601 gcggcgttcc ggcgatcagg ctcgggatcg cgaacaggat tccgatcgcg acggtcagcg
     3661 agaccgccca gatcttccac cgcgggaagt cgagcatcag tcgttggccg gcttggcaga
     3721 cttggtgacg acgtggctga gcgtcgcctt gacgatccgc acccgcacat tctggccgat
     3781 ctcgacctcg acgtcgtcgt ccatcacctt ggtgacccgg ccgcggatgc cgccgccggt
     3841 aatcacctca tcgcccttct tgaccgcggc gatggtcgcc gcatgctcct tcatccgctt
     3901 ttgctgcggg cggatcatca acagatagaa gatcacgaag atcagcagcc acggcaggat
     3961 cccgacgaag atcgccgacg ggtcgctcgc cggggtggcg gtcatcaaaa ggggggtggt
     4021 cataggcgcg tgtcgtcttt ccaatggacg ggatggtcgg ggcgactgga ttcgaaccag
     4081 cgacccccac acccccagtg tgatgcgcta ccaggctgcg ctacgccccg accagttgcc
     4141 gcggcccgtg tcgataggac aagggaacgg cgacgcggca tctagtcagc cgggtccttc
     4201 aaggcaaggc gcaagcggcg gacgagtggt gcctcgaaga cgaaaaaggg atggcgcggc
     4261 ccgggctacg acgcgacgcg cagagccggc ctctcgacat ggcagccgta gcgggagatg
     4321 agcgcgggga tctcctgcgc gcgcaccggg cggctgatgt ggaagccctg cgcctcggtg
     4381 cagccttcgg cggccagcag gtgcagctgc ccggccgtct cgattccttc cgcggtcgtg
     4441 ccgatgccaa gcttttcgga cagctggatg gtcgcctgga cgatcgccag cgcatcgcgg
     4501 gtcaccgtga tgtccgacac gaacgagcgg tcgatcttga tcttgctgaa cgggaagctg
     4561 cgcagatagc tgaggctcga gtagccggtg ccgaaatcgt ccatggcgag ccggaccccg
     4621 aggtcgcgca gcttgcacag cacgttccgc acccagattt cgtcattgag aagcaccgat
     4681 tcggtgatct cgagctcgag ccgtccagat tcgagccccg ccgccttcag cgcggcggtc
     4741 gtcatcgcca ggagatcgcc gcgcttgaac tgcgcggccg acagattgac cgcgacgctg
     4801 atcggcttgg gccaattcgc cgcctcgatg caggcctggc ggagcaccca ttcgccgatc
     4861 ggaacgatca gcccgctttc ctccgcgacc gggatgaagt cgctcggttg aagcatcccg
     4921 cgcctcggat ggtgccagcg gaccagcgct tccatgccgc aaatcctgcg gctcgagagg
     4981 ttcaatagag gctggtaata cagctcgaac tggccgtttt gcacggccgc ccgaagatcg
     5041 ttgccaagcg cccggcgctc attgaggtgc ttgtccatgc gcggcttgta gaagcagaag
     5101 ccgccccgtc cgacattctt cgcccaatag agcgcgaggt cgcttttctt gagaagctcg
     5161 gcgccgtccc ggctgtcctt cggcgcgagg gcaattccga cgctggcgcc gatggcgatc
     5221 gtgtgcccat cgatctcgaa cgggttggac aagccggcga ttatcctgtc ggcaagcaac
     5281 tccgcctggc gaggctgctt gatgcgccgc tggatcaccg cgaattcgtc tccggcgagg
     5341 cggctgacga actcgtcctt gccggcaatg tcgcgcaacc gcaccgcggc ctgcttcagg
     5401 agctcgtcgc cgatcggatg gccgagcgtg tcgttgac
//
LOCUS       DASTZO010000301         2278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_45171,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000301 DASTZO010000000
VERSION     DASTZO010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2278
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45171"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="VFU20_08455"
     CDS             <1..782
                     /locus_tag="VFU20_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013053873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5286519.1"
                     /translation="DAADPLAALERRAQADPDNAGAWQELGFAYFELGRFADAATAYD
                     KATRADPDSAVLWSSLGEARVMASARDPMPPAALEAFRKAAALDSGDPRARYFLAVQK
                     DLAGDHPGAIADWLALLADTPPGAPWEADLRRTIEQVGKINKIETASRLAAVKQPVPA
                     GPPPTAVASLPGPTAEQVQDASKLSPSQQMDMGRSMVERLEGRLKTDPSNVGGWIMLM
                     RSRMTLKEPQAAKAALAAAIAANPGERARLEAEARALGVPN"
     gene            complement(905..>2278)
                     /locus_tag="VFU20_08460"
     CDS             complement(905..>2278)
                     /locus_tag="VFU20_08460"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5286520.1"
                     /translation="REAPIRTVPAWKILNDEIAPEKLAELFSQSIVYVGTSAPGLQDL
                     VSTPLDARLPGVMVHAQATEQILLGKFLTRPYDAHAYERWLTLGLGLLMALLLPWIGA
                     TRGAVLGIVMVGGMAAGSWFAFSQRQMLIDPTYPILGLILVWVSVTAFTYYREEKQRA
                     YIHQAFDRYLSPDLVKRIVDDPSRLQLGGEEREMTVLFCDIRGFSRISEKLGPQEIIR
                     FLIAFLTPMCDILLARRATIDKFIGDAILAFWNAPLDDPDQYGNAARGALAMIERLKQ
                     LNREMPGGAEPWPGDVAIGIGLNSGPCCVGNMGSAQRLSYSLIGDTVNLASRIEGLTK
                     YYGVTIAMGEDLQARLREFATIELDRVRVVGRDAPETVHALIGDEVLGADAAFRAFAA
                     DHDAMLLAYRAQDWDGAVALLDKGGGAAAGYGLGKLYALIGERIAGYRETSPGADWDG
                     VYGATEK"
BASE COUNT          388 a          837 c          727 g          326 t
ORIGIN      
        1 cggacgccgc cgacccgctc gccgcgctcg agcggcgcgc gcaggccgac cccgacaacg
       61 ccggcgcgtg gcaggagctc ggcttcgcct atttcgagct cggccgcttc gccgacgccg
      121 ccacggccta cgacaaggcc acgcgcgccg accctgacag cgcggtgctg tggtcctcgc
      181 tcggcgaggc gcgggtcatg gccagcgcgc gcgacccgat gccccccgcc gcgctcgagg
      241 cgttccgcaa ggccgccgcc ctcgactccg gcgacccccg cgcgcgctac ttcctcgcgg
      301 tgcagaagga cctcgcaggc gaccacccgg gcgccatcgc cgactggctc gcgttgctcg
      361 ccgacacgcc gccgggtgcg ccgtgggaag ccgatctccg gcggacgatc gagcaggtcg
      421 gcaagatcaa caagatcgag acggcgtcgc ggctggcggc ggtgaagcag cctgtgccgg
      481 ccggcccgcc gcccactgcc gtcgccagcc tgccgggacc gactgccgaa caggtgcagg
      541 acgcgtccaa gctctcgccc agccagcaga tggacatggg ccgctccatg gtcgagcggc
      601 tggaggggcg gctcaagacc gatccgtcga atgtaggcgg ctggatcatg ctgatgcgca
      661 gccggatgac gctgaaggaa ccccaagccg ccaaggccgc gctggcggcg gcgatcgcgg
      721 cgaacccggg cgaacgggcg aggctggagg cggaggctag agcgctgggg gtccccaact
      781 aatccggcca agccggaaga agatcgattc cgcaaagacg cgaagacgca aagagcgtta
      841 cccctggcga gacatcttcg cgtcttcgcg gaaatctaaa ttccccggct tcgccgggag
      901 ggtcttactt ctcggtcgcc ccgtacaccc catcccaatc cgcgcccggc gaggtctcgc
      961 gatagccggc gatccgctcg ccgatcaggg cgtacaattt ccccagccca tagcccgccg
     1021 ccgcaccccc gcccttgtcg agcaacgcca cagccccgtc ccaatcctgc gcgcgatacg
     1081 ccagcagcat cgcgtcatga tccgccgcga aggcgcggaa ggcggcgtcc gcgcccagca
     1141 cttcgtcgcc gatcagcgcg tgcaccgtct ccggcgcgtc gcggccgacc acgcggacgc
     1201 ggtcgagttc gatcgtcgcg aattcgcgga ggcgcgcctg cagatcctcg cccatcgcga
     1261 tcgtcacgcc ataatatttg gtcagtccct cgatccgcga ggccaggttc accgtgtcgc
     1321 cgatcagcga atagctcagc cgctgcgccg accccatatt gccgacgcag cacggccccg
     1381 aattgagccc gatcccgatc gccacatcgc ccggccaggg ctcggccccg cccggcatct
     1441 cgcggttgag ctgtttcaga cgctcgatca tcgccagcgc cccccgcgcg gcattcccat
     1501 actggtccgg atcgtcgagc ggtgcgttcc agaaggcgag aatcgcgtcg ccgatgaact
     1561 tgtcgatcgt cgccctgcgc gccagcagga tgtcgcacat cggggtcagg aaggcgatga
     1621 ggaagcggat gatctcctgt ggccccaatt tctccgaaat ccgcgaaaag ccgcgaatgt
     1681 cgcagaacag caccgtcatc tcgcgctctt cgccgcccag ctggagccgg ctgggatcat
     1741 cgacgatgcg cttgaccaga tctggcgaca ggtaccggtc gaacgcctgg tggatgtacg
     1801 cgcgctgctt ttcctcgcga taataggtga aggccgtgac cgagacccag accaggatca
     1861 gccccaggat cggataggtc ggatcgatca gcatctgccg ctggctgaac gcgaaccagc
     1921 tgcccgccgc catgccgccc accatgacga tcccgagcac cgccccgcgc gtcgcgccga
     1981 tccacggcag cagcagcgcc atcagcaggc ccagccccaa ggtcagccag cgctcatagg
     2041 catgcgcgtc gtacggccgc gtcaggaact tgccgagtag gatctgctcg gtcgcctggg
     2101 catggaccat caccccgggc agccgcgcgt cgagcggcgt ggagacaagg tcctgcagcc
     2161 ccggcgcgct cgtaccgaca tagacgatcg actggctgaa cagttcggcc agtttctccg
     2221 gcgcgatctc gtcgttcaat atcttccacg cgggcaccgt gcggatcggc gcctcgcg
//
LOCUS       DASTZO010000302         5854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1017162,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000302 DASTZO010000000
VERSION     DASTZO010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017162"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1164
                     /locus_tag="VFU20_08465"
     CDS             <1..1164
                     /locus_tag="VFU20_08465"
                     /inference="COORDINATES: protein motif:HMM:NF003715.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HEU5286521.1"
                     /translation="RAMVYVLNNLNLAQGLHAPFVAATVIVIFGLASVVHGSGFLAVY
                     LAGLVVGNRPTRAHNTVVVFLDAVTWLAQIVMFVLLGLLVWPQRLLETLLPALAIAAT
                     LMLIARPAAVFLCLWPFRFPWRETAFISWVGLRGAVGIFLASIPLLVGVPNAYRYFDI
                     AFVVVLTSLLIQGWTIAPAAKRLGVSFPRGKPLPRRVELDLPGQLEQEMVGYPVVAKS
                     PYLRRGLIPAWAKPTLIVRDERILTAAEGSPVREGDYLYLLAPPEKAEALDRFFVDMP
                     PPAAPDPRLLGDFFVSGEVTLNALAEIYGLSVAEKDAGVSLAEFFADELKRTPRQGDI
                     VPLGPIALLAQRVANGRVITVGLRLAEEDEPPAPTTLAGRAKHVARQIVARLK"
     gene            complement(1176..1967)
                     /locus_tag="VFU20_08470"
     CDS             complement(1176..1967)
                     /locus_tag="VFU20_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019053062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5286522.1"
                     /translation="MIATQATAAALTATGLSVRLARKVIVEDASLSLRAGEFTVLLGP
                     NGAGKTTLLRALAGVLPADGHIEIGGRPLQTLSLRERARRIAYLPQGHVTHWPMDVAS
                     VVALGRHPHADAFAPPTADDRAAVDAALTATGLEPLAARSVTSLSGGERARVALARAL
                     ATRASILLADEPTMSLDPRHQLVVMELLVQVARRGTAVLAVLHDLALAARFADRVLMM
                     DCGRLVADGSPREVLTPDKIATVFGVEAAITETGVGAIPILRRPI"
     gene            complement(1964..2992)
                     /locus_tag="VFU20_08475"
     CDS             complement(1964..2992)
                     /locus_tag="VFU20_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5286523.1"
                     /translation="MWRASTNRSGLIVALSALLVVLAILSLMIGPAGLSPREALAGLI
                     DGEGTAGIIVRSIRLPRTLLALLIGATLGLSGAALQGLLRNPLAEPALFGAPQAAAAG
                     ASFVIAYGLAGATSFAVPAAGILGALVSIGGLVAIAGRRASLTVTLLAGLAFASFAGA
                     ATALILNLAPSPYAALEIAFWLLGSLEDRSMEHVWIALPFFAACWVLLFLNLPAYRAL
                     TLGEDAAASLGVDIDRTRLMVVIGVALGVGAAVAVAGAIGFIGLVAPHLVRRAVGSDP
                     ARVCVPAMLTGAALLTAADIAVRLIPTNPNATAVELKVGVVTALIGVPFFLLMIFSER
                     RYGDGASS"
     gene            3127..3948
                     /locus_tag="VFU20_08480"
     CDS             3127..3948
                     /locus_tag="VFU20_08480"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5286524.1"
                     /translation="MAGAAVFASCAWATAAEPPQRVVSFNVCADQLVVALADPSQIAG
                     LSPYATDPAVSTVVEKAQQFRRLGWQAESTIPLDPDLVLVGSWDRSLSKRLLRALGFR
                     VVEVDVVADLAAARAQIREFAALLGHPQRGEALIAEIDAAQRRLSEAGRGASSTALLV
                     GNGGYTVGPQSLAAALMNEAGFKAPPGSPSGYGGFVPLERLIELHPDVLVVSNMLEIP
                     DGQGAIYLTHPALQELYPPSRRVLLPSRYTLCGGPSLIAAFDYLTEVVTRLGRKP"
     gene            complement(3963..4757)
                     /locus_tag="VFU20_08485"
     CDS             complement(3963..4757)
                     /locus_tag="VFU20_08485"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HEU5286525.1"
                     /translation="MFPPSRIVCLTEETVETLYLLGEEHRIAGVSGYAVRPARVRKEK
                     PRVSAFISADYEKVLALEPDLVLTFSDLQADIAAELIRRNVAVHAFNQRDIAGILAMV
                     RTLGALVGAAAKAEALARSLERRLSEVAALTAQTTRRPRVYFEEWDEPMISGIRWVAE
                     LIEVAGGIEAFPELSVHKSAKDRIVSVPDVIAAQPDIIVGSWCGKKFVPAKVAARPGF
                     DRVPAVRDGFLREIKSPLILQPGPAALTDGLDALAAIVAEWSAQNA"
     gene            complement(4832..5173)
                     /locus_tag="VFU20_08490"
     CDS             complement(4832..5173)
                     /locus_tag="VFU20_08490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286526.1"
                     /translation="MLGMSARSRTNWDEIERRLNSVESTLRHDGRRLAKAVPSTDQVG
                     DLVATVLNGVAERFRGVSVSDEAARLGNEAAKFGNYAVTRVSKEVAHRPLIALGVAVG
                     VGVLLGLASRR"
     gene            5479..>5854
                     /locus_tag="VFU20_08495"
     CDS             5479..>5854
                     /locus_tag="VFU20_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007011956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5286527.1"
                     /translation="MASPTPIAGDYDYIIVGAGSAGCLLANRLSADPDKRVLLLEAGG
                     NDNWIWFHIPVGYLFAIGNPRSDWLFKTEPEEGLNGRSLNYPRGKAIGGSSSINAMIY
                     MRGQAADYDHWRQLGLTGWGYDD"
BASE COUNT          911 a         1989 c         2027 g          927 t
ORIGIN      
        1 cgggccatgg tctatgtgct caacaatctc aacctcgcgc aggggctgca cgcgccattc
       61 gtcgccgcga ccgtcatcgt gatcttcggc ctcgcctcgg ttgtgcacgg ctcaggcttc
      121 ctcgcggtct atctggcggg cctcgtggtc gggaatcggc caacccgcgc gcacaacacc
      181 gtcgtggtgt ttctcgatgc cgtcacctgg ctcgcccaga tcgtgatgtt cgtgttgctc
      241 gggctgctgg tctggcccca gcggctgctg gaaaccctgc tgccggcgct ggcaatcgcc
      301 gccacgctga tgctcatcgc ccgtccggcg gctgtcttcc tgtgcctgtg gccctttcgc
      361 ttcccgtggc gggaaaccgc attcatttcc tgggtcgggc tgcgcggtgc ggtcggaatc
      421 tttcttgcct cgattccgct gctggtcggc gtgcccaacg cgtaccgcta cttcgacatc
      481 gccttcgtgg tggtgctgac gtcgctgctg atccagggct ggacgattgc gccggccgca
      541 aaacgcctcg gcgtgtcgtt tccgcgcggc aagcccctgc cgcggcgcgt ggaactcgat
      601 ctgccgggac agctcgagca ggagatggtc ggatacccgg tcgtcgccaa gagtccctat
      661 ctgcggcgcg gcctcattcc ggcctgggcg aagccgacgc tgatcgtgcg ggacgagcgc
      721 atcctgaccg ccgccgaggg ctcgccggtg cgcgaaggcg actaccttta tctgttggcg
      781 cctcccgaga aggcggaggc gcttgaccgt ttcttcgtcg acatgccgcc gccagccgcg
      841 cccgacccgc ggctccttgg cgatttcttc gtctccggcg aggtgacgct caatgcgctg
      901 gccgagatct acgggctgtc ggtggcggaa aaggatgccg gcgtttcgct cgcggaattt
      961 ttcgccgacg agctcaagcg cacgccccgg caaggcgaca tcgtgccgct cggcccgatc
     1021 gccttgctgg cgcagcgggt cgcgaacggg cgcgtcatca ctgtcggcct gcgcctcgcc
     1081 gaggaagatg agccgccggc gccgacgacg ctcgcgggcc gcgcgaagca tgtcgcacgc
     1141 cagatcgtcg cgcggctaaa gtaacggcgc cacgctcaga tcggccggcg caggatcggg
     1201 attgcaccca cgccggtttc ggtgatcgca gcctcgacgc cgaaaaccgt cgcgatcttg
     1261 tcgggtgtga gaacctcgcg cggggagccg tccgccacca gccggccgca atccatcatc
     1321 agcacgcggt cggcaaagcg cgcggcgagc gcaaggtcgt gcagtacggc gaggaccgcg
     1381 gtcccgcggc gcgccacctg taccagcagc tccatgacga cgagttgatg ccgcggatcg
     1441 agcgacatcg tcggctcgtc ggcgagcaag atcgaggctc gggtcgccag cgcccgcgcc
     1501 agcgccacgc gggcgcgttc gccgcccgac agcgaggtga cgctacgcgc tgccagcggt
     1561 tcgagcccgg tcgcggtgag ggctgcatcg acggcagcgc gatcgtctgc ggtcgggggc
     1621 gcaaaggcgt cggcatgggg atgccgtccg agcgcgacga ccgaggcgac gtccatcggc
     1681 cagtgcgtta cgtggccttg cggcaggtag gcgatgcggc gcgcccgctc gcgcagcgac
     1741 aaggtctgga gcggtcgtcc gccgatctcg atgtggccgt ccgccggcag aacgccggcc
     1801 agcgcgcgga gcagcgtggt cttgccggcg ccgttcggcc cgagcagcac ggtgaattcg
     1861 ccggcgcgca gcgagagcga cgcgtcttcg acgatgacct tgcgggcgag ccggaccgac
     1921 aggccggttg ccgtcaacgc agccgcggtc gcctgcgttg cgatcacgat gacgctccgt
     1981 cgccgtaccg ccgctcggag aagatcatca gcaggaagaa cggcacgccg atcagcgcgg
     2041 tgacgacgcc gaccttgagc tcgaccgcgg tggcattcgg attggtcgga atgagccgca
     2101 ccgcgatatc ggcagcggtc aagagtgcgg cgccggtcag catcgccggc acgcagaccc
     2161 gcgccggatc ggagccgacg gcgcgccgca ccaggtgcgg cgccacgagc ccgatgaagc
     2221 cgatggcgcc ggcgaccgcc accgccgcgc ccacgccgag cgcgacgccg atcacgacca
     2281 tcagccgggt gcggtcgatg tcgacgccga gcgacgcggc ggcgtcttcg ccgagcgtca
     2341 gcgcgcggta ggccggcagg ttgaggaaca gcagcaccca gcaggcggcg aagaacggca
     2401 gcgcgatcca cacgtgctcc atgctgcggt cttcgagcga accgagcagc cagaacgcga
     2461 tttcgagtgc cgcgtaaggg gagggggcga ggttgaggat gagcgcggtg gcggcgcccg
     2521 cgaagctcgc gaaggcgaga cccgcgagca gggttacggt gaggctggca cgccggccgg
     2581 cgatcgcgac caggccgccg atcgaaacca gcgcgccgag gatgccggcc gccggcaccg
     2641 cgaacgaggt ggcgcccgca agcccatagg cgatgacgaa cgacgcgccc gcggcggcgg
     2701 cctgcggcgc gccaaacagc gccggttcgg cgagcggatt acgcaacagc ccttgcagtg
     2761 ctgcgccgga aaggcccagg gtggcgccga tcaggagcgc cagcagggtg cgcggcaagc
     2821 ggatgctgcg cacgatgatg ccggccgtgc cctcgccgtc gatcaggccg gcgagcgcct
     2881 cgcgaggcga cagcccggcg ggcccgatca tcagcgacag gatggcgaga accaccagca
     2941 gcgccgaaag tgcgacgatc aggcctgaac ggtttgtgct cgcgcgccac atgcaatacg
     3001 gcttccctgg gcggcccttc ctacaggctt cgggcttgtg gtgaaacggg cttgggcggc
     3061 ttcagtgggg cgatgttcgc agggagggaa tttggagtga ggcgatccgc ggtttgttcc
     3121 tatctggtgg cgggcgccgc ggtatttgcg tcgtgcgcat gggctaccgc cgccgagccg
     3181 ccgcagcggg tcgtgtcgtt caacgtctgc gccgaccagc tcgtggtcgc gctcgccgat
     3241 ccgtcgcaga tcgccggcct ctcaccctat gcgaccgacc cggccgtctc gacggtcgtc
     3301 gagaaggcgc aacaattccg ccggctcggc tggcaggcgg aatcgaccat cccgctcgat
     3361 cccgacctgg tgctggtcgg ttcttgggac cgctcgttgt ccaagcggct gctgcgggcg
     3421 ctgggctttc gcgtcgtcga ggtcgacgtc gttgccgacc ttgccgcggc tcgcgcgcag
     3481 attcgcgagt tcgccgcgct gctcgggcat ccgcaacgcg gcgaggcgct gatcgccgag
     3541 atcgacgccg cacagcgccg gctgtcggag gccggacgcg gtgcgtcctc gacggcgctt
     3601 ctcgtcggca atggcggcta cacggtcggg ccccaaagcc tcgcggccgc actgatgaac
     3661 gaggctgggt tcaaggcgcc gccgggctcg ccgtccggct atggcggctt cgttccgctt
     3721 gagaggctga tcgaactgca tcccgatgtg ctggtcgtat ccaacatgct cgaaatcccg
     3781 gacgggcagg gcgccatcta tctcacgcat ccggcgctgc aggagctgta tccgccgtcg
     3841 cggcgggttc tgctgccgag ccgctatacg ctgtgcggcg gaccgagtct gatcgccgcg
     3901 ttcgactatt tgaccgaggt ggtgacccgg cttggtcgca agccgtagct gaaagatgag
     3961 tgtcaggcgt tctgcgccga ccattcggca acgatggcgg cgagcgcatc gaggccgtcg
     4021 gtgagtgccg cgggcccggg ctgcaggatc agcggcgatt tgatctcgcg caggaagccg
     4081 tcgcgcactg ccggcacccg atcgaagccc ggccgcgccg ccactttcgc gggcacgaat
     4141 ttctttccgc accacgagcc gacgatgatg tccggttggg cggcgatcac gtccggcacc
     4201 gacacgatgc ggtccttggc gctcttgtgc acggacagtt cgggaaacgc ctcgatcccg
     4261 cccgccacct cgatcagctc ggcgacccag cggatgccgg aaatcatcgg ctcgtcccat
     4321 tcctcgaaat agacccgcgg ccgccgcgtg gtttgcgccg tgagggccgc gacctccgac
     4381 aaccgccgtt ccagcgatcg cgccagcgcc tcggccttgg cggcggcgcc gaccagtgcg
     4441 ccaagcgtgc ggaccatcgc cagaatcccc gcgatgtccc gctggttgaa ggcgtggacc
     4501 gcgacgttgc ggcggatcag ctcggctgcg atatcggcct gcaggtcgga gaaggtgaga
     4561 acgagatccg gttcaagcgc cagcaccttc tcgtagtcgg ccgagatgaa cgccgagacc
     4621 cgcggcttct ccttgcgcac gcgggccggc cggaccgcat agccggagac accggcgatg
     4681 cggtgctctt cgccgagaag atagagcgtc tcgacggttt cctcggtcag gcagacgatt
     4741 cgcgacggcg gaaacatccg ccgagcttac tggcgcggat gcggcgcgac gagagcctcc
     4801 ggccgttggt acgcgttcag cgggtacccg ctcagcggcg gctggcaagt cccagcagga
     4861 cgccgacgcc gaccgcgacg ccaagcgcga tcagcggacg gtgcgcgacc tccttcgaca
     4921 cgcgggtgac cgcgtaattg ccgaacttcg ccgcctcgtt gccgagccgc gccgcctcat
     4981 cgctgaccga aacgccgcgg aaccgttcgg cgacgccgtt cagcaccgtc gcgacaaggt
     5041 cgccgacctg atcggtggac ggcaccgcct tcgcgagccg cctgccgtcg tgtcgcagcg
     5101 tgctttcgac cgaattgagc cggcgttcga tctcatccca atttgtgcgg gaccgtgcgg
     5161 acataccaag catgtgtgct ctctcctgat gaagcggcca tggaaaacgg atatcgggcg
     5221 cgcgggcgca ggccacgcgg tcgtcgtctc cctgagaacc ggcagacggc gggaaagttc
     5281 catcgtggcg gtgacggccg ggaacgacag gctcaattcc gtccgcggtg tcgagcggtt
     5341 cgaaccgccc tgctgatcat aacccgcata tcggcaaagc cgtcgcagtc aatgcagtca
     5401 tgcacccggc ttcggggaca ttgccaagtt ccaggcacca ccgcagactg ctgtcgttta
     5461 cagccggggt gcgaaatcat ggcttcgccg acgcccatcg cgggcgacta cgactacatc
     5521 atcgttggcg ccggctcggc cggttgcctg ctggcgaacc gcctctccgc cgatccggac
     5581 aagcgcgtcc tgctgctgga ggccggcggg aacgacaact ggatctggtt ccacatcccg
     5641 gtcggctatc tgttcgccat cggcaatccg cgctccgact ggctgttcaa gaccgagccg
     5701 gaggaagggc tcaacggccg cagcctgaat tatccgcgcg gcaaggcgat cggcggctca
     5761 tcctcgatca acgccatgat ctacatgcgc gggcaggctg ccgattacga ccactggcgg
     5821 cagctcgggc tcaccggctg gggctacgac gacg
//
LOCUS       DASTZO010000303         4378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_332072,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000303 DASTZO010000000
VERSION     DASTZO010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4378
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_332072"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(236..1543)
                     /locus_tag="VFU20_08500"
     CDS             complement(236..1543)
                     /locus_tag="VFU20_08500"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5286528.1"
                     /translation="MEKQHKIGLGAFALLLGAAYVTMRSQPAEQSGPVPAAQANTHLA
                     AVTPAARRDIDYAALDARLKRLAEQRPVVGLAVGIVENGRITFLSGYGETLAESGEPV
                     TPDTVFRWASVSKGVAATMIAKLAEERKLSFNSPLVQYASMKLPGGAEARATIGDLLS
                     HRLGLYRNAYDNKLEEGQDTRFLRRTLAHLNLICPPGTCWSYQNVAYDGSSEIVERVT
                     GKPYAEAVREKLFNPIGMTSASVTRDGLVGARSWARPHDAASRPLEVLDTYYRVPAAG
                     GVNSNIKDMALWMLAQMGRMPGVLSPQLLQTIHAPLVKTPGERRRLRKFLERLGQADY
                     GYGWRSYDYAGHRIVGHRGGVSGYRSLILFDPDRKSGVVAMWNSNSNQPGGLEFEVMD
                     MLYRLPFRDWMELDGRPGRAKPAGAEPAEDSAAIAARTTAPRG"
     gene            1706..1999
                     /locus_tag="VFU20_08505"
     CDS             1706..1999
                     /locus_tag="VFU20_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA
                     domain-containing protein"
                     /protein_id="HEU5286529.1"
                     /translation="MQFLKTLFWVLLAVFIALFASRNWSDVTLNLWGDIQVDIKIPVL
                     LAVMFLAGFLPVFLVHRARLWTMQRRIQAQERQQSAAPGPAAVPAHADDHPVT"
     gene            1996..2679
                     /gene="pyrF"
                     /locus_tag="VFU20_08510"
     CDS             1996..2679
                     /gene="pyrF"
                     /locus_tag="VFU20_08510"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU5286530.1"
                     /translation="MSPIFVAIDTADPARAREIARAVRGVAGGLKLGLEYFAANGPAG
                     VAELAELGLPIFLDLKLHDIPNTVAKAVQALRPLEPAILTVHAAGGRAMLENAKAAAS
                     PGTKVVAVTVLTSLDGKDLAAAGIKGSPADQVARLAALAHSAGLDGIVCSGEEVASAR
                     KAWPDGFFVVPGVRPEGGGAGSGAADQKRTVTPRSAIDGGASILVIGRPVTEADDPSA
                     AVRSIAATL"
     gene            2741..3595
                     /gene="accD"
                     /locus_tag="VFU20_08515"
     CDS             2741..3595
                     /gene="accD"
                     /locus_tag="VFU20_08515"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011240486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HEU5286531.1"
                     /translation="MSWLSRVRKGIPFLPKRQTTDNLWHKCGKCGTMVFLKEWEDNLR
                     VCPRCDHHDRVGPKARFDQLFDAARYELLPAPEVREDPLRFKDSKRYTERLKAARAAT
                     DERDALLNARGTVDDHPAVVGVQDFAFMGGSMGIAVGAAFVAGVRAAIAEQCPYVLFT
                     AAGGARMQEGILSLMQMPRTTVALAELREAGLPYIVVLTDPTTGGVTASYAMLGDVQL
                     AEPGALIGFAGQRVIEQTIREKLPEGFQRAEYLLAHGMIDMVVPRHRLRERLSQLIAY
                     LAPEKAAA"
     gene            3732..>4378
                     /locus_tag="VFU20_08520"
     CDS             3732..>4378
                     /locus_tag="VFU20_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014848694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEU5286532.1"
                     /translation="MADFARSAHPAVQAQLDRLARLSPIGDRLGLERIRLLLDRLGRP
                     QDALPPVLHVAGTNGKGSTCAFLRAALEASDRTVHMFTSPHLVRYNERIRIAGRLIED
                     EALAGLLAEVLDSSGGIEASFFEVTTAAAFLAFARTPADACIVEVGLGGRLDATNVVE
                     RPLVCGIASLAVDHQQFLGRRMAGIAAEKAGIAKPGAPLVTQLYPPAIAARIGEVA"
BASE COUNT          619 a         1606 c         1496 g          657 t
ORIGIN      
        1 gtggaccgac aattcgaggc agatctgacc ggcgagcttc tgcttacggc cctcgccgct
       61 ggctttctcg aattgcgcga acaggtcttc gacagtgcgg tggtcctgct tgagcagcgc
      121 gatcgcatcc tgggccttcg attccggcat tgggtcctcc tgctgggtgc cggaagaacc
      181 cgccgctcgg gacacaagtt cctaggattg cgggcttaat cgattcaacc ggagcctagc
      241 cgcgcggcgc cgtcgtcctc gccgcgatcg ccgcgctgtc ctcggccggc tccgcaccgg
      301 ccggcttcgc ccgcccgggc cggccgtcga gctccatcca gtcgcggaac ggcagccggt
      361 agagcatgtc catgacttcg aactcgagcc cgccgggctg gttggagttg ctgttccaca
      421 tggcgacgac gccgctcttg cggtccgggt cgaacaggat cagcgaacgg tagccgctga
      481 ccccgccgcg gtgcccgacg atccggtggc cggcataatc gtagcttcgc cagccgtagc
      541 cgtagtcggc ctggcccagc cgctcgagga acttgcgcag ccgccgccgc tcgccggggg
      601 tcttgaccag cggcgcgtgg atggtctgca gcaactgcgg cgacagcacg ccgggcatcc
      661 ggcccatctg ggcgagcatc cacagcgcca tgtccttgat gttgctgttg accccgcccg
      721 ccgccggcac ccggtaataa gtgtcgagca cttcgagcgg ccggctggcg gcatcgtgcg
      781 gccgcgccca gctcctcgcg ccgaccagcc cgtcgcgggt gacgctggcg ctggtcattc
      841 cgatcgggtt gaacaatttc tcgcggaccg cctcggcata gggcttgccg gtcacccgct
      901 cgacgatctc cgacgagccg tcgtaggcga cgttctggta gctccagcag gtgccgggcg
      961 ggcagatgag gttgagatgg gcgagagtcc gccgcaggaa gcgcgtgtcc tgcccttcct
     1021 cgagcttgtt gtcgtaggcg ttgcggtaaa gcccgagccg gtggctcaag aggtcgccga
     1081 tcgtcgcccg cgcctcggcc ccgcccggca gcttcatgct cgcgtactgg acgagcggcg
     1141 agttgaagct cagcttgcgc tcctcggcga gcttggcgat catcgtcgcg gcgacgccct
     1201 tcgacaccga cgcccagcgg aacacggtgt ccggggtcac cggctcgccc gattcggcga
     1261 gcgtttcgcc atagccactc aggaaggtga tgcggccgtt ctcgacgatc ccgacggcga
     1321 gcccgaccac cggccgctgc tcggccagcc gcttcagccg cgcgtcgagc gccgcataat
     1381 cgatgtcgcg gcgggcggcc ggagtcaccg cggcgagatg cgtgttggcc tgcgcggccg
     1441 ggaccggccc cgactgctcc gccggctgcg agcgcatggt cacataggcc gcgcccagga
     1501 gcagcgcgaa cgcacccaaa ccgatcttgt gctgcttctc caagccagct cccctccacc
     1561 gcgtccctct gtggagtcga tggtgaaccg gctcaacgcc gagccctgca agctgcgatg
     1621 aacggatttg gtggcgcgac gaatgccgct cgtgctggcc atcgccgccc cgctcgccta
     1681 aaggcccgcg aggggagcga cgccgatgca atttctgaag accctgttct gggtgcttct
     1741 cgccgtcttc atcgccttgt tcgccagccg caactggtcc gacgtcacct tgaacctgtg
     1801 gggcgacatc caggtcgaca tcaagatccc ggtgctgctg gcggtgatgt tcctggcagg
     1861 cttcctgccg gtgttcctcg tccaccgggc gcggctgtgg acgatgcagc ggcgcatcca
     1921 ggcgcaggag cggcagcaat cggccgcgcc ggggccggcc gcggtgccgg cgcacgcgga
     1981 cgaccatccg gtgacatgag cccgatcttc gtcgccatcg acacggccga ccccgcccgc
     2041 gcgcgcgaga tcgcgcgcgc cgtgcgcggg gtcgccggcg ggctcaagct cggcctagaa
     2101 tatttcgccg ccaacgggcc ggccggggtc gccgagctcg ccgagctcgg gctgccgatc
     2161 ttcctcgatt tgaagctcca cgacattccc aacaccgtcg ccaaggcggt ccaggcgctg
     2221 cggccgctgg agccggcgat cctgaccgtc cacgccgccg ggggccgggc gatgctcgag
     2281 aacgccaagg ccgccgcctc gccggggacc aaggtcgtcg cggtcaccgt cctcaccagc
     2341 ctcgacggca aggacctcgc cgcggccgga atcaagggca gcccggccga ccaggtcgcg
     2401 cgcctcgccg cgctcgcgca cagcgccggc ctcgacggca tcgtctgctc gggcgaggag
     2461 gtcgcctcgg cgcgcaaggc gtggcccgac ggctttttcg tcgtccccgg ggtccggcca
     2521 gagggcggcg gcgccggcag cggcgcggcc gaccagaagc gcaccgtcac cccgcgctcg
     2581 gcgatcgacg gaggcgcgtc gatcctggtc atcggccggc cggtcaccga ggccgacgat
     2641 ccctccgccg cggtccggag catcgccgcg acgctctagg cggtggacaa gcggccgcgc
     2701 ttgcgcctag cggggccgcg aggaccggaa gaggatttcg atgagctggc tgagccgcgt
     2761 ccggaagggc atcccgttcc tgcccaagcg gcagacgacc gacaatctct ggcacaagtg
     2821 cggcaagtgc gggacgatgg tcttcctcaa ggaatgggag gacaacctcc gggtctgccc
     2881 gcgctgcgac caccacgacc gggtcggccc caaggcccgg ttcgaccaat tgttcgacgc
     2941 cgcgcgctac gagctgctgc cggcgcccga ggtccgcgag gacccgctgc ggttcaagga
     3001 cagcaagcgc tacaccgagc gcctcaaggc cgcccgcgcc gccaccgacg agcgcgacgc
     3061 cttgctcaac gcgcgcggca cggtggacga ccatccggcc gtcgtcgggg tccaggactt
     3121 cgccttcatg ggcgggtcga tgggaatcgc ggtcggcgcc gccttcgtcg ccggggtgcg
     3181 cgcggcgatc gccgagcaat gcccgtacgt gctgttcacc gccgccggcg gggcgcggat
     3241 gcaggaaggc atattgtcgc tgatgcagat gccgcggacg acggttgccc tggccgagct
     3301 gcgcgaggcg ggcctgccct acatcgtcgt cctcaccgac ccgaccaccg gcggcgtcac
     3361 cgcctcctat gcgatgctcg gcgacgtcca gctcgccgag cccggcgccc tcatcggctt
     3421 cgccggccag cgggtgatcg agcagaccat ccgcgagaag ctccccgagg gcttccagcg
     3481 cgccgaatat ctgctcgccc acgggatgat cgacatggtc gtcccgcgcc accggctgcg
     3541 cgagcgcttg tcgcagctca tcgcctatct cgcgccggag aaggcagcag cgtgacgagc
     3601 agctcctccc cggaacgggg agggggacca ttcgccgcag agcgaatggt ggagggggcg
     3661 gcgagcctcg ctcgggtccc cctccgtcag cgatgcgcgc tgccacctcc ccgtcccggg
     3721 gaggcggcct gatggccgac ttcgcccgct ccgcccaccc cgccgtccag gcccagctcg
     3781 accgcctcgc caggctctcc ccgatcggcg accggctcgg gctcgagcgc atccgcctgt
     3841 tgctcgaccg cctcggccgg ccgcaggacg cgctgccgcc ggtgctccac gtcgccggga
     3901 ccaacggcaa gggctcgacc tgcgcgttcc tccgcgccgc gctggaagcc tcggaccgaa
     3961 ccgtccacat gttcaccagc ccgcacctgg tccgctacaa cgagcgaatc cggatcgccg
     4021 ggcgcctgat cgaggacgaa gcgctcgccg gcctgctcgc cgaagtgctc gactcgagcg
     4081 gcggcatcga ggcgagcttt ttcgaggtca cgacggcggc cgccttcctc gccttcgcgc
     4141 gcacgcccgc cgatgcgtgc atcgtcgagg tcgggctcgg cggccggctc gacgcgacca
     4201 atgtcgtcga gcggccgctg gtgtgcggga tcgccagcct ggcggtcgac caccagcaat
     4261 tcctcgggcg gcggatggcc ggcatcgccg ccgagaaagc cggcatcgcc aagccggggg
     4321 cgccgctggt cacgcagctc tacccgccgg cgatcgccgc tcggatcggc gaggtcgc
//
LOCUS       DASTZO010000304         8040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_45253,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000304 DASTZO010000000
VERSION     DASTZO010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 8040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8040
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45253"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..194)
                     /locus_tag="VFU20_08525"
     tRNA            complement(118..194)
                     /locus_tag="VFU20_08525"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(158..160),aa:Arg,seq:cct)
     gene            complement(231..638)
                     /locus_tag="VFU20_08530"
     CDS             complement(231..638)
                     /locus_tag="VFU20_08530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HEU5286533.1"
                     /translation="MIVFDLCCLQSGDRFEAWFRSSADFDEQLEQGLVHCPVCESSRV
                     AKAPMAPMVPRRSAPAAGAVERLAELQADLLRGSEWVGDAFADTARAMHLGEIDTSPV
                     HGEATLEQARSLLDDEVPILPLPLPVVPPKQVN"
     gene            complement(635..1465)
                     /locus_tag="VFU20_08535"
     CDS             complement(635..1465)
                     /locus_tag="VFU20_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HEU5286534.1"
                     /translation="MARIALFQSCTGIDPAANARALVDAVGQAADGGAEMLFTPEMSG
                     LLDRDPERAAGNLRAEDEDEVLAACREAAKSRGLWLHLGSLAVRTDGQKLANRTFVID
                     PSGAIRARYDKIHLFDVDLPTGESWRESAVYRPGEGAVVVDGTPVGRLGLAICYDLRF
                     PDLFSRLAEAVAETVAVPAAFTVPTGRAHWEVLLRARAIEAGAFVVAAAQSGRHADGR
                     ETYGHSLVVGPWGELLLDMGDGTGVGFADMDLSRVGEVRRRIPSLTHRRPIAQAAVVQ
                     "
     gene            complement(1469..1726)
                     /gene="grxC"
                     /locus_tag="VFU20_08540"
     CDS             complement(1469..1726)
                     /gene="grxC"
                     /locus_tag="VFU20_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HEU5286535.1"
                     /translation="MPRVEIYTKSFCGYCFRAKRLLDSKGIEYQEHAVHYGGPERQDM
                     IQRSGGRTTVPQIFIDGRHIGGCDELMELESRGKLDELLAA"
     gene            complement(1758..2504)
                     /locus_tag="VFU20_08545"
     CDS             complement(1758..2504)
                     /locus_tag="VFU20_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEU5286536.1"
                     /translation="MIRGAALKAGKIARWILDFALPPRCAGCGTIVTDVHSFCPDCWK
                     RIEFLGSGGCVTCGTPLQATDSECCAVCLARPPRIERTRAAVAYDDLTRSLALRLKYG
                     RKVAIAKTMARYMAPLVGTQPGSALLVPVPLHRGRLWQRGFNQSAIVARELSRQTGID
                     SDATLLKRIRRTPPLKGMSLQQRRRVVAGAFRVGDRSAVDGRTVILVDDVLTTGSTAN
                     ACARELRRAGAARVELVSWARVVRPSHLVR"
     gene            2549..3352
                     /locus_tag="VFU20_08550"
     CDS             2549..3352
                     /locus_tag="VFU20_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU5286537.1"
                     /translation="MPTEIFDMELRALRRDRAFRTGPELFLHERAFEDCLERLALVRR
                     RFKSALLVGCPNPGWPGRLNQFAETVEAADPGPLFATAAGALCVVEDQWDPPVSAYDL
                     ILAVGTLDTVNDLPRALRTLRAALEEDSLLIGAMAGGETLPRLRSAIRAADRAAGGSS
                     PHVHPRIEAAALSPLLAACGFVMPVVDVDRVQTSYPTLASLVTDLRRMGSTSILKRRS
                     RPPILRRSKAAAEAAFGSAGDGARTVETFEILHFAAWTPPLRSRPGNQG"
     gene            3439..3624
                     /locus_tag="VFU20_08555"
     CDS             3439..3624
                     /locus_tag="VFU20_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008066563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HEU5286538.1"
                     /translation="MIAIRTTLRRLLADQGGATAIEYGLIAALIAVAAIGGMSALGGG
                     AGGMWTDLSSEVQNAAS"
     gene            complement(3640..5109)
                     /locus_tag="VFU20_08560"
     CDS             complement(3640..5109)
                     /locus_tag="VFU20_08560"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007709988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HEU5286539.1"
                     /translation="MRRPFLLLAATAIVLAPAASQAQQGRYIDPREVAEAQRQHAELL
                     SEFGGAETGARAAYVESVGRRLAAYSGVANAGGVFRFTTLNSAVENAFAVPGGYVYIT
                     RQLMTLMDDESQLAFALGHETGHIAANHHRARQSAARRNSISGIFGTLLGSLLGGGIG
                     NVIAQLSQQQAQLATLSFSRDQEYQADTLATRYMIGAGYDPAGGPGVLAALGRASAIQ
                     ARVQGRSNRQTPEWASTHPLSENRLQRALAAARATGRLGQGMRNRDQFLAQLDGIYVD
                     DDPAQGIIEGRSFTHPDLRIQFAVPMGYLMQNGTRAVTIKGSAGQAQFSGGRYGGSIE
                     NYIYQVFQQLTGGRQQMMIPPPQRTMVNGFPAAFTTARANTSSGVVDVSVMAYQWDPN
                     TIYHFIMLTRGGAGIGPFVQMVESLRRITPAEAASIRPRVIDVVTVRPGETVHSLAGR
                     MAYRDFQLDRFLALNGLTVATPLVPGQKVKLIVYGARRS"
     gene            complement(5191..6132)
                     /locus_tag="VFU20_08565"
     CDS             complement(5191..6132)
                     /locus_tag="VFU20_08565"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEU5286540.1"
                     /translation="MLTFLDFEKPIAELETRVAELRETATAGSIDIDTEIARLEAKSA
                     KLLRETYSRLTPWQKSQVARHPERPHFNDYVAGMADDFVPLAGDRAFGDDPAIVGGLA
                     RIDGRRVMIIGHEKGDDTASRLKHNFGMAKPEGYRKAIRLMRLADRFGIPIVTLVDTP
                     GAFPGVEAEERGQAEAIARSTEQCLEVRVPIIAAIVGEGGSGGAVAIAAANRVLMFEH
                     AVYSVISPEGCASILWRTADKAAEAAEAMKITASDLQALGVVDRIVAEPLGGAHRDPD
                     AAIGAIKGAILEELDGCSALGGDELRTQRRAKFLAIG"
     gene            complement(6191..7078)
                     /locus_tag="VFU20_08570"
     CDS             complement(6191..7078)
                     /locus_tag="VFU20_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase"
                     /protein_id="HEU5286541.1"
                     /translation="MTDDDRALVDRFLDMMAAEAGASPHTLAAYRNDLKRAAEDAGGS
                     LGEAGAEQLSRLGKQWAGLSPATVARRSAALRRFFGFLMDEGFRSDDPSAALPRPRLE
                     RPLPRILDEREVTAMFEAAEDRAASGEPAAVRNLAVLELLYGSGLRASELVTLGRSSL
                     RKGQPFVILRGKGGKERLVPISSRAEAAVGRWLEFVPGDSLWLFPSGKRHLSRVRLFQ
                     IVREVAALAGIAPERVSPHVLRHAFATHLLSGGADLRVLQSLLGHADIATTQIYTHVD
                     SARLVELVNARHPLADRDR"
     gene            complement(7089..>8040)
                     /locus_tag="VFU20_08575"
     CDS             complement(7089..>8040)
                     /locus_tag="VFU20_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286542.1"
                     /translation="TIEWEPVDRLNSWRFGLATATGMTLPDRLLRSASPQIRAWQARA
                     PLLSPQERLESARIAAGLGVLSSQSLIDLYSLIYDATDPDQLSETDAWQVRLAFVGRD
                     RDARLAAIRRLWSIGDGPLQDEASRALAARAATRIDPDPDLQEDAPALIASMLAGGYD
                     REAARWALAVRRMDDRFADESWAMLALAAPETRGLDLSFGRVNAFISRDQSRGKKRSA
                     LLVAGLAGLGRLDGRSADRLNRRHGLAIGHRSRWTRMIDGSAALGQAGSVLVLAGAGF
                     QATGFEHLPSSHLYHSVAALNRTGQGFTARMIAAEALART"
BASE COUNT         1155 a         2876 c         2812 g         1197 t
ORIGIN      
        1 atgccgagcg accgcgggtg gtcggtgaac agcctgccga ccatcgatga gccctcctga
       61 ccgccgctgt cttgccgtcc tgaagccatc gtcctgctcc gcccctagcg accgagttgg
      121 tggccccggc aggactcgaa cctgcgacct gcggtttagg aaaccgttgc tctatcctgc
      181 tgagctacgg agccaatggg cgcccgaata gagcacgaga gcccgcttga tcaattgact
      241 tgcttgggcg gcacgaccgg cagcggcagg ggcaggatcg gcacctcgtc gtcgagcagc
      301 gagcgcgcct gctccagagt cgcctcgccg tgcaccgggc tggtgtcgat ctcgcccaga
      361 tgcatcgccc gggcggtgtc ggcgaacgcg tccccgaccc attcggagcc gcgcagcagg
      421 tcggcctgaa gctcggccaa ccgctcgacc gctccggctg ccggggcgct ccggcgcggc
      481 accatcggcg ccatcggcgc cttggccact cggctcgatt cgcagaccgg gcaatggacc
      541 agcccttgtt ccagttgctc gtcgaagtcg gcgctcgacc ggaaccaggc ctcgaaccgg
      601 tcgccgcttt gcaggcagca aaggtcgaag acgatcattg cacgaccgcc gcctgcgcga
      661 tcggccggcg atgggtcagc gacgggatcc gccgccgaac ctcgccgacc cgggacaggt
      721 ccatgtccgc gaacccgacc ccggttccgt cgcccatgtc gagcagcagc tcgccccacg
      781 ggccgaccac cagcgaatgg ccgtaggtct cgcggccgtc ggcgtggcgg cccgactgcg
      841 ccgcggcgac gacgaacgcg cccgcctcga tggcgcgagc gcgcagcagg acctcccaat
      901 gcgcccggcc ggtcggaacg gtgaacgccg ccggcaccgc gaccgtctcg gccacggcct
      961 cggcgagcct cgagaacagg tcggggaagc gcaggtcata gcagatcgcc aggcccagcc
     1021 gcccgaccgg cgtcccgtcg accaccacgg cgccttcgcc cgggcgatag acggcggact
     1081 cgcgccagct ttcgccggtc ggaaggtcta cgtcgaacag atggatcttg tcgtagcgcg
     1141 cccggatggc tccgctcggg tcgatgacga aggtccggtt ggcgagcttc tgcccgtcgg
     1201 tgcgaaccgc cagcgacccc aggtgcagcc acagcccgcg gctcttcgcc gcctcccggc
     1261 acgcggcgag cacctcgtcc tcgtcctcgg cgcggaggtt tcccgcggcg cgctccggat
     1321 cgcggtcgag cagccccgac atctccggag tgaacagcat ctcggcgccg ccgtccgccg
     1381 cttggccgac cgcatcgacc agcgcgcggg cgttcgcggc cgggtcgatc ccggtgcacg
     1441 actggaaaag agcgatccgc gccatcgctc aggcggccag caattcgtcg agcttgcccc
     1501 ggctttccag ctccatcagc tcgtcgcagc cgccgatgtg gcggccgtcg atgaagatct
     1561 gcgggacggt ggtccggccg cccgaacgct gaatcatgtc ctgcctctcg ggcccgccat
     1621 aatgcaccgc atgctcctga tactcgatcc ccttggaatc gagcaggcgc ttggctcgaa
     1681 agcagtagcc gcagaacgac ttggtataga tttccactct tggcacgcgc ggcctttcgt
     1741 cactcactgg agcgtcgtca acgcaccaga tgcgaaggcc gcaccactcg cgcccagctg
     1801 accagctcga cccgggccgc ccccgcccgc cgcaattcgc gggcgcaggc gttcgcggtg
     1861 cttccggtgg tcagcacgtc gtcaaccagg atcaccgtcc ggccgtcgac cgccgagcgg
     1921 tcgccgaccc gaaaggctcc ggcgacgacg cgccggcgct gctgaaggct catccccttg
     1981 agcggcggag tgcgccggat gcgcttcagc aaggtcgcat cgctgtcgat gccggtctgg
     2041 cgggacagct cgcgggcgac gatcgccgac tggttgaagc cgcgctgcca caggcggccg
     2101 cggtgcagcg gcaccggcac cagcagcgcg ctccccggct gggtcccgac gagcggcgcc
     2161 atgtagcggg ccatcgtctt ggcgatcgcg accttgcgcc cgtatttgag ccgcagcgcg
     2221 aggcttcggg tgaggtcgtc atacgccacc gcggcgcggg tccgctcgat ccgcggcggc
     2281 ctcgccaggc acaccgcgca gcattcggaa tcggtcgcct gcagcggagt cccgcaggtc
     2341 acgcagcctc ccgaaccgag gaactcgatg cgtttccagc aatcggggca gaagctgtgg
     2401 acatcggtga cgatcgtgcc gcagccggcg caccgcggcg gcagcgcgaa gtcgaggatc
     2461 catcgggcga tcttgccggc cttgagtgct gcgcctcgga tcatattgcc gcttgtcggc
     2521 cttgcgcgtg cgccgcacaa gctcttccgt gcccacggaa atattcgaca tggaattgcg
     2581 ggcgctgcgc cgcgaccggg cgttccgcac cggccccgag ctgttccttc acgaacgggc
     2641 gttcgaggat tgcctcgagc ggctcgcgct tgtccgccgc cggttcaaat cggccctgct
     2701 ggtcggctgc ccgaaccccg gctggcccgg ccggctgaac cagttcgccg agaccgtcga
     2761 ggccgcggat cccgggcctt tgttcgccac ggccgccggc gcactttgcg tggtcgagga
     2821 ccaatgggac ccgccggtat cggcctacga cctcatcctt gcggtcggga ccctcgacac
     2881 cgtcaacgat ttgccgcgag cgcttcggac gctccgcgcc gcgctcgagg aggattcgct
     2941 cctgatcggg gccatggcgg gcggcgagac gcttccccgg ctgcggtccg cgatccgggc
     3001 cgccgaccgg gccgcgggag gatcgtcgcc gcatgtccac ccacggatcg aggccgcggc
     3061 tttgtcgccg ctcctggccg cgtgcggctt cgtcatgccg gtggtcgacg tcgaccgggt
     3121 gcaaacctcc taccccaccc tcgcaagcct ggtgacggac ctgcggcgga tgggctcgac
     3181 cagcatcctc aaacgccggt cccgaccccc catcctgcgc cggtcgaaag cggcggcgga
     3241 ggcggcgttc ggttcggccg gggacggagc gcgaaccgtc gagacgttcg agatcctgca
     3301 tttcgcggcc tggactccac cgctccggtc gcggcccggg aatcagggct gaaggcccgt
     3361 taacgatggg cgctgggtaa gttgttaact caccccgggt tagtgtgcgg tcgtggacgt
     3421 ggaccagagg agggtggcgt gatcgctatc cggacgacct tgcgcaggtt gctcgccgac
     3481 cagggcggag cgacggcaat cgaatatggg ctgatcgcgg cattgatcgc ggtcgcggcg
     3541 atcggcggca tgtcggccct cggcggcggc gccggcggca tgtggaccga cctcagcagc
     3601 gaggtccaga acgccgcttc gtaaccgcgg cggggcggct caggaccgcc gcgctccgta
     3661 gacgatcagc ttcactttct gcccggggac cagcggggtc gccaccgtca ggccgttcag
     3721 cgccaggaat cggtcgagct ggaagtcgcg gtaggccatc cggccggcca gcgaatgcac
     3781 cgtctcgccc gggcggaccg tcaccacgtc gatcacccgc gggcggatcg aagccgcctc
     3841 cgccggagtg atccggcgca gcgattcgac catctggacg aacgggccga tcccggcgcc
     3901 gccgcgggtc agcatgatga agtggtagat ggtgttgggg tcccactgat aggccatgac
     3961 gctgacgtcg acgacccccg acgaggtgtt cgcccgggcg gtggtgaagg ccgccggaaa
     4021 gccgttgacc atcgtccgct gcggcggcgg gatcatcatc tgctggcggc cgccggtcag
     4081 ctgctggaac acctggtaga tgtagttctc gatgctcccg ccgtagcggc cgccgctgaa
     4141 ttgcgcttgg cccgccgaac ccttgatggt caccgcgcgg gtgccgttct gcatcagata
     4201 gcccatcggc accgcgaact ggattcgcag gtcggggtgg gtgaacgagc gcccctcgat
     4261 gatcccctgc gccgggtcgt cgtcgacata gatgccgtcg agctgggcga ggaactggtc
     4321 gcggttgcgc attccctggc cgaggcgccc ggtggcgcgc gccgcggcga gcgcgcgctg
     4381 caggcggttc tcgctcagcg ggtgggtgct ggcccattcc ggcgtctgcc ggttgctccg
     4441 gccctggacc cgcgcctgga tcgcgcttgc ccggccgagc gcggcgagga ctccggggcc
     4501 gcccgccggg tcgtagccgg cgccgatcat gtagcgcgtc gccagcgtgt ccgcctgata
     4561 ctcctggtcg cgcgagaagc tcagcgtcgc cagctgcgcc tgctgctggc tcaattgggc
     4621 gatcacattg ccgatgccgc cgccgagcag gcttccgagc agggttccga agatcccgct
     4681 gatcgagttg cggcgggccg ccgactgccg agcgcggtgg tggttggcgg cgatgtggcc
     4741 ggtttcgtgg ccgagcgcga aggcgagctg ggactcgtcg tccatcaagg tcatcagctg
     4801 gcgcgtgatg taaacatagc cgccgggcac cgcgaaggcg ttctcgaccg ccgagttcag
     4861 ggtggtgaag cggaacaccc cgccggcgtt ggcgaccccg gaataagcgg cgaggcggcg
     4921 gccgaccgat tcgacatagg ctgctcgggc cccggtctcg gcgccgccga actccgagag
     4981 cagctcggcg tgctggcgct gggcctcggc gacctcgcgc gggtcgatgt atcggccttg
     5041 ctgggcctgc gacgccgcag gcgcgaggac gatcgcggtc gccgcgagca gcaggaaagg
     5101 tctacgcata ccaacctccg gtttggatcg cccgtttaga aaccgggcat tgaacgggag
     5161 atgactgctt gtggttccgg aacgacccgg tcagccgatc gccaggaact tggcccttcg
     5221 ctgcgtccgg agctcgtcgc cgcccagcgc cgagcaaccg tcgagctcct cgaggatggc
     5281 gcccttgatc gccccgatcg ccgcatcggg gtcgcggtgg gcgccgccca gcggctcggc
     5341 gacgatgcgg tcgacgaccc cgagcgcctg gaggtcggac gcggtgatct tcatcgcctc
     5401 ggccgcctcc gcagccttgt cggcggtgcg ccacaggatc gacgcgcagc cttccggcga
     5461 aatcaccgaa tagaccgcat gctcgaacat cagcacccgg ttggcggccg cgatcgcaac
     5521 cgctccgccc gacccgccct cgccgacgat ggcggcgatg atcggtaccc ggacctcgag
     5581 gcattgctcg gtcgagcggg cgatcgcctc ggcctggccg cgctcctcgg cctcgacccc
     5641 ggggaacgcc ccgggcgtgt cgaccagcgt gacgatcggg atcccgaaac ggtcggcgag
     5701 ccgcatcagc cggatcgcct tgcggtagcc ctcgggcttg gccattccga aattatgctt
     5761 gagccgcgac gccgtgtcgt cgcccttctc gtggccgatg atcatcaccc gccggccgtc
     5821 gatccgggcc agtccgccga cgatcgccgg gtcgtcgccg aacgcccggt cgccggccag
     5881 cggcacgaag tcgtcggcca tcccggcgac ataatcgttg aagtgcgggc gctcggggtg
     5941 ccgcgcgacc tgtgacttct gccacggggt cagccgcgaa taggtctcgc ggagcagctt
     6001 ggccgatttc gcctcgaggc gggcgatctc ggtgtcgatg tcgatcgagc cggcggtggc
     6061 ggtctcgcgc agctcggcga cgcgcgtctc gagctcggcg atcggctttt cgaagtcgag
     6121 gaaagtcagc ataggcgcgg gcggcgggta ggacgcgcct cccgatccgt caacgacccc
     6181 gcccgatcgc tcagcggtcg cggtccgcga gcggatggcg cgcgttgacc agctcgacca
     6241 ggcgcgcgct gtcgacgtgg gtgtagattt gcgtcgtcgc gatgtcggca tggccgagca
     6301 gggactgcag cacccgaagg tcggcgccgc ccgacagcaa atgggtcgcg aacgcgtgcc
     6361 ggagcacgtg cgggctgacc cgttcgggtg cgatcccggc cagcgccgcc acctcgcgga
     6421 cgatctggaa cagccgcacc cggctcaaat ggcgcttgcc gctcgggaac agccacaggc
     6481 tgtcgccggg gacgaattcg agccagcgcc cgaccgccgc ttccgcccgc gaggagatcg
     6541 gcaccagccg ttccttgccg cccttgccgc gaaggatcac gaacggctgg cccttgcgca
     6601 gcgagctgcg gccgagcgtg accagctcgc tggcccgaag ccccgatccg tagagcagct
     6661 cgagcaccgc gaggttgcgc accgccgccg gctcgccgct cgcggcgcgg tcctcggcgg
     6721 cttcgaacat cgccgtcacc tcgcgctcgt cgaggattcg cggcagcggc cgctcgagcc
     6781 tcggccgcgg cagcgccgcc gacgggtcgt cgctccgaaa gccctcgtcc atcaggaagc
     6841 cgaagaaccg gcgcagcgcc gccgagcgcc gggcgacggt cgccggcgac agccccgccc
     6901 attgcttgcc caggcgcgac aattgctccg ctccggcctc gccgagcgac ccgccggcgt
     6961 cctcggccgc gcgcttcaga tcgttgcggt aggcggcgag cgtgtgcggc gaggccccgg
     7021 cctcggccgc catcatgtcg aggaaccggt cgaccagcgc ccgatcgtcg tcggtcacgc
     7081 cttgtccgtc acgtgcgcgc gagcgcctcg gcggcgatca tcctggcggt gaagccttgg
     7141 ccggtgcggt tgagcgcggc gaccgaatgg tagaggtgcg aagagggaag atgctcgaag
     7201 ccggttgcct ggaagccggc cccggccagc accagcacgc tgcccgcctg gccgagcgcc
     7261 gccgagccgt cgatcatccg ggtccagcgg gagcggtggc cgatcgccag cccgtggcgg
     7321 cggttgagcc ggtcggcgct ccgcccgtcg aggcgcccga gcccggccag accggcgacc
     7381 agcagggcgc tgcgcttctt gccgcggctc tggtcgcggc tgatgaacgc gttgacccgg
     7441 ccgaagctca ggtccaggcc ccgggtctcg ggcgcggcca gggccagcat cgcccagctc
     7501 tcgtcggcga agcgatcgtc catgcgccgg acggcgagcg cccagcgcgc cgcttcgcgg
     7561 tcgtagccgc cggccagcat cgaggcgatc agcgccggcg catcttcctg gaggtcggga
     7621 tcggggtcga tccgagtcgc ggcccgcgcg gccagcgccc gcgaggcctc gtcctgcaac
     7681 gggccgtcgc cgatcgacca cagccggcgg atcgccgcca ggcgggcatc gcggtcgcgg
     7741 ccgacgaacg ccaggcgcac ctgccaggcg tcggtctccg aaagctggtc ggggtcggtc
     7801 gcgtcgtaga tcagggaata caggtcgatc agcgactggg acgacagcac cccgaggccg
     7861 gccgcgatcc gggcggactc gagccgctcc tgcggcgaca gcagcggcgc ccgcgcctgc
     7921 cacgcccgga tctgcgggct cgccgaccgc agcaggcgat cgggcagggt catgccggtc
     7981 gccgtcgcca gcccgaatcg ccagctgttg aggcgatcga ccggctccca ctcgatggtc
//
LOCUS       DASTZO010000305         4252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1278564,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000305 DASTZO010000000
VERSION     DASTZO010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278564"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..116)
                     /locus_tag="VFU20_08580"
     CDS             complement(<1..116)
                     /locus_tag="VFU20_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU5286543.1"
                     /translation="MFTGITTDVGTVRSAEQRGDLRLTIACGYDMATVDLGAS"
     gene            complement(127..1080)
                     /gene="ribD"
                     /locus_tag="VFU20_08585"
     CDS             complement(127..1080)
                     /gene="ribD"
                     /locus_tag="VFU20_08585"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU5286544.1"
                     /translation="MAEAVALGGAMRGRTAPNPNVGCVIVSGGAVVGRGATRPGGRPH
                     AEAVALAEAGELALGATLYTSLEPCAHRSGRGPACADLIVEAAPAKVVVALRDPDPRT
                     AGKGIERLRSAGIAVKLGTGRTAAAQSMSGYLTRLKLGRPRITLKLALSIDGKIALPS
                     GESKWITGEDARAHVHLERARSDMILVGRGTYLADAPRLDVRLPGLEDRSPRRALLTR
                     GEAVDGWETLRSPQDVYGLHDVNDLLVEGGSATATAFLAEDLVDRILIYRAPIIVGEG
                     KSSFGYVGLDGIAVAHGRWRAGDARRLGVDRLEVYERVRDS"
     gene            complement(1110..1430)
                     /locus_tag="VFU20_08590"
     CDS             complement(1110..1430)
                     /locus_tag="VFU20_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286545.1"
                     /translation="MSWFPSPVSPRAAFADFRTFVRHREREHWIGAALAVLVTIIIVI
                     EFFVDSKINTAPPPQIVYVEQWKTDRTDAEIIADQKKRQAEKEAAARERQRQFQRLEK
                     QLGM"
     gene            complement(1519..2175)
                     /locus_tag="VFU20_08595"
     CDS             complement(1519..2175)
                     /locus_tag="VFU20_08595"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU5286546.1"
                     /translation="MLAYAAHRRRIAQRHSAPHVMLAIIAGHIALIAAVMSARMDLPE
                     RILHPPTVVTPIPLPDPPPPEPAQPAPRPSPFTVTRVPAIVPVPQPNRPDPPVLLPLP
                     NPGPVGLMPVPQPGVGPLPHDPVRVGPRFATPASLVKPPYPPSKLNSEEEAVLRLRLS
                     IDARGRVVAVEPVGRADPAFLAAARRHLIAHWRYRPASEDGRAIASATVITLRFELEG
                     "
     gene            complement(2264..3583)
                     /locus_tag="VFU20_08600"
     CDS             complement(2264..3583)
                     /locus_tag="VFU20_08600"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEU5286547.1"
                     /translation="MSVVTRFAPSPTGRLHVGNIRTALHNALFARRHGGAFILRIDDT
                     DRERSTVEFDQAIRDDLDWLGLKPDSVVRQSERFGLYEREFERLVDAGRVYACYETPE
                     ELELRRKVLLGRGLPPVYERKPEGAPVPEGRAPHWRFRLDHGRPIEWRDLVRGPQRFD
                     PKLLSDPVVRREDGSWLYLLPSVIDDVDLGITHIVRGEDHVSNSAAQLQMFDALGAQP
                     PELAHEALLVAAEGKLSKRLGSIGVEEIRGAGLEPMALLSLLARLGTSRPVEALASLD
                     ELAADFDFAHFGRAPAHFDLHDVELLNARLLHRLDYAAVADRLPDCASEADWLLLRGN
                     LERLSDFAAWHAVIAGDIEPPSLGADERALVGEAAAVAAGLDWSDDPWHALADALKAA
                     TGRKGKALFHPLRLALTGRESGPEMAGLLRRMGKERALARLAAAANP"
     gene            complement(3580..>4252)
                     /gene="nadE"
                     /locus_tag="VFU20_08605"
     CDS             complement(3580..>4252)
                     /gene="nadE"
                     /locus_tag="VFU20_08605"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006952280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HEU5286548.1"
                     /translation="LEDAEGCAKLLGCRHDVIPIVPAVQAFGEMLPDLQGVAAENIQA
                     RLRMVALMALSNANGHMLLTTGNKSEMSVGYATLYGDMAGGYSVLKDAYKTTVFALSR
                     WRNRVRPDGVLGPDGPVMPARVISKAPTAELRPGQKDQDSLPPYSLLDRVLEGLVDKE
                     MSVKEVALATGAEIALVADIETKLLRAEYKRRQAPPGVKIGSRNFGRDRRYPITNFFH
                     TRPKK"
BASE COUNT          605 a         1473 c         1570 g          604 t
ORIGIN      
        1 gacgcgccca gatcgaccgt cgccatgtcg tagccgcagg cgatggtgag ccgcaggtcg
       61 ccgcgctgct cggcgctgcg gaccgtgccg acgtcggtgg tgatgccggt gaacatggcc
      121 cgcgccttac gagtcccgca cccgctcgta aacttcgagc cggtcgaccc cgagccggcg
      181 ggcgtcgccg gcccgccagc ggccgtgcgc cacggcgatc ccgtcgagcc cgacatagcc
      241 gaacgacgac ttgccctcgc cgacgatgat cggggcccgg tagatcagga tccggtcgac
      301 caggtcctcg gcgaggaaag cggtcgcggt cgccgagccg ccctcgacca acaggtcgtt
      361 gacgtcatga agtccgtaca catcctgcgg ggaacgcagg gtttcccagc cgtcgaccgc
      421 ctctccccga gtcagcagcg cgcgcctcgg cgagcggtcc tcgagccccg gcaggcgcac
      481 gtcgagccgc ggcgcgtcgg ccaaataggt gccgcggccg accaggatca tgtccgagcg
      541 cgcgcgctcg aggtggacgt gggcgcgcgc gtcctcgccg gtgatccatt tcgattcgcc
      601 cgacgggagc gcgatcttgc cgtcgatcga cagcgccagc ttcagggtga ttcgcggccg
      661 gcccagcttg aggcgcgtca aatagccgga catggattgc gccgcggccg tccggccagt
      721 ccccagcttc acggcgatgc ccgccgagcg cagccgctcg atgcccttgc cggccgtgcg
      781 gggatcgggg tccctgagcg cgacgaccac tttggccggc gccgcctcga cgatcaggtc
      841 ggcgcaggcg gggccgcgcc cgctccggtg ggcacagggc tcgaggctgg tgtaaagcgt
      901 cgccccgagc gcgagctcgc cggcctcggc caaagcgacg gcctcggcgt gcgggcgccc
      961 gcccggccgg gtcgctccgc ggccgacgac cgcgccgccc gagacgatca cgcagccgac
     1021 attggggttg ggggcggttc ggccgcgcat tgcgccgccg agcgcgaccg cttcggccat
     1081 gaagcgagcc ccgttgcccc cgtcgtcgct cacatcccga gctgtttctc taatctttgg
     1141 aactggcgct ggcgctccct ggccgcggcc tccttctcgg cctgccgctt cttctggtcg
     1201 gcgatgatct cggcgtcggt gcggtcggtt ttccactgct cgacatagac gatctgcggc
     1261 ggcggcgcgg tgttgatctt cgaatcgacg aaaaattcga tgacgatgat gatcgtcacc
     1321 agcaccgcga gcgcggcgcc gatccaatgc tcgcgctcgc ggtggcggac gaaggtgcgg
     1381 aagtcggcga acgccgcgcg cgggctgacc ggacttggaa accaggacat gggaggaaga
     1441 taggccttcg aagcggcgct cgccagcccc ggtgcggcaa acctgctcgc tttgtccccc
     1501 ggacagcgcg gcgcgggctt acccctcgag ctcgaagcgc agcgtgatca ccgtcgcgga
     1561 ggcgatcgcc cgcccgtcct cgctcgccgg ccggtagcgc cagtgggcga tcaggtggcg
     1621 gcgcgcggcg gcgaggaagg cgggatcggc gcggccgacc ggctcgaccg cgaccacccg
     1681 gccgcgcgcg tcgatcgaca gccgcagcct cagcaccgct tcctcctcgc tgttgagctt
     1741 ggacggcggg tagggcggct tcaccaggga ggccggggtc gcgaaacgcg ggccgactcg
     1801 caccgggtcg tggggcagcg ggccgacgcc gggctgcggg accggcatca gcccgaccgg
     1861 gccgggattg ggcagcggca gcaggaccgg cgggtcgggc cggttgggtt gcggcaccgg
     1921 gacgatcgcc gggacccggg tgacggtgaa cggcgagggc ctcggcgccg gctgcgccgg
     1981 ctcgggcggc ggcgggtcgg gcagcgggat gggggtgacg acggtcggcg ggtgaaggat
     2041 gcgctcgggc aggtccatcc gggcgctcat caccgcggcg atcagggcga tgtggccggc
     2101 gatgatcgcc agcatgacgt gcggggccga gtggcgctgg gcgatccggc gccggtgagc
     2161 tgcgtaggcc agcattgccg atctcctgca acgcgagtcg atgaagcaat gttacatcat
     2221 atcataccag gcggcaacgg gggtcgtcgc ggcggcgcgg cgatcaggga ttggcggcgg
     2281 cggcgaggcg ggccagcgcg cgctccttgc ccatgcgtct gagcagcccg gccatctccg
     2341 ggccggactc gcgcccggtc agcgccaggc gcagcggatg aaagagcgcc ttgcccttgc
     2401 gcccggtcgc ggccttgagc gcgtcggcga gcgcgtgcca cgggtcgtcc gaccaatcga
     2461 ggccggcagc gaccgccgcg gcctcgccga ccagcgcgcg ctcgtcggcg ccgaggctgg
     2521 gcggctcgat gtcgccggcg atcaccgcgt gccaggcggc gaagtccgac agccgctcga
     2581 ggttgccgcg aagcagcagc cagtccgcct cgctggcgca gtcgggcagc cggtccgcga
     2641 ccgccgcata gtcgagccgg tgcagcagcc gggcgttgag caattcgacg tcgtgcaggt
     2701 cgaagtgcgc cggggcgcgg ccgaaatggg cgaagtcgaa atcggcggcg agctcgtcga
     2761 ggctcgccag cgcctcgacc ggccgagacg tgccgagccg ggcgagcagc gacagcaagg
     2821 ccatcggctc gagccccgcg ccgcggatct cctcgacccc gatcgagccc aggcgcttcg
     2881 acaatttgcc ctccgcggcg accagcagcg cctcgtgcgc gagctcgggc ggctgagcgc
     2941 caagcgcgtc gaacatctgc agctgggcgg cgctgttcga gacatggtcc tcgccgcgca
     3001 cgatgtgggt gatgccaagg tcgacgtcgt cgatcaccga ggggagcagg tagagccagc
     3061 tgccgtcctc gcggcggacc accgggtcgg acagcagttt cgggtcgaag cgctgcgggc
     3121 ctcggaccag gtcgcgccac tcgatcggcc ggccgtggtc gagccggaac cgccagtggg
     3181 gcgcgcggcc ctctgggacc ggcgcgccct cgggcttgcg ctcgtagacc ggcggcaggc
     3241 cgcggccgag cagcaccttg cggcgaagct cgagctcctc gggagtctcg tagcaggcat
     3301 agacccggcc cgcgtcgacc agccgctcga actcgcgttc gtagaggccg aagcgctcgg
     3361 actggcgaac gacgctgtcc ggcttcaagc ccagccagtc gaggtcgtcg cggatcgcct
     3421 ggtcgaactc gacggtcgag cgctcgcggt cggtgtcgtc gatcctgagg atgaacgcgc
     3481 cgccgtgccg gcgcgcgaac aaggcgttgt ggagcgcggt acggatgttg ccgacgtgca
     3541 ggcgcccggt cggcgacggc gcgaagcgcg tgacaacgct catttcttcg gccgcgtgtg
     3601 gaagaaattg gtgatcgggt agcggcggtc gcggccgaaa ttgcggctgc cgatcttgac
     3661 cccgggcggc gcctggcggc gcttgtactc ggcgcgcagc agcttggtct cgatgtcggc
     3721 gaccagcgcg atctcggcgc cggtggcgag cgcgacctcc ttgaccgaca tttccttgtc
     3781 gaccagcccc tcgaggaccc ggtcgagcag cgaataaggc ggcaggctgt cctggtcctt
     3841 ctggccgggg cgcagctcgg cggtcggcgc cttgctgatc acccgcgccg gcatcaccgg
     3901 gccgtcgggg ccgagcacgc cgtcgggccg gacccggttg cgccaccgcg acagggcgaa
     3961 caccgtcgtc ttgtaggcat ccttcaagac cgaatagccg cccgccatgt cgccgtagag
     4021 ggtcgcatag ccgaccgaca tctcgctctt gttgccggtg gtcaggagca tgtgcccgtt
     4081 tgcattcgac agcgccatta gcgccaccat gcgcagtcgg gcctggatat tctcggccgc
     4141 gaccccctgc aggtcgggca acatctcgcc aaaggcctga accgccggga caatcgggat
     4201 gacgtcatgg cggcagccga gcagcttcgc gcagccttcc gcgtcctcga gg
//
LOCUS       DASTZO010000306         3000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_102135,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000306 DASTZO010000000
VERSION     DASTZO010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102135"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VFU20_08610"
     CDS             complement(<1..1070)
                     /locus_tag="VFU20_08610"
                     /inference="COORDINATES: protein motif:HMM:NF020134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] synthase III
                     C-terminal domain-containing protein"
                     /protein_id="HEU5286549.1"
                     /translation="MRPVQILGTGGHLPGDPIDNATLERLVGPLPDDVMEGISIQQRF
                     WMTDPFTGAHRENNSDMAYTAARMALESANVDAGEIDLMILATGTPDYHLPPVVNFVQ
                     DRLGIGQCATMELRSGGAGVVQGLDIAKMYLERGVYKTALVIGSETISPVLIPVYLGK
                     SPDAVRMRERMPLYMFGDGAGAMVLRAGEEGAPGIVGAAQACVGGGKKPGIHAVGGGT
                     HAPIAEQLKARRLVDLRVDVLEAGKFTPYMITQAIGETLRRSGVGVQEIDLCLIPEGN
                     VGWMLDSMEEGGLTTADWAALDGKIFDNLAQSGATGCAAVPLFLDHAWRHGQVKPGDR
                     IMLVGLESTKWIYAGAVVDWTAP"
     gene            complement(1114..>3000)
                     /locus_tag="VFU20_08615"
     CDS             complement(1114..>3000)
                     /locus_tag="VFU20_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HEU5286550.1"
                     /translation="IGYAAELIRSGQADAVLTGGADALSDVLFAGFNCLESLSPEPAA
                     PYSKDRQGLSLGEGSGMLVLVREDVAERIGAPVLAEVLGYALSADGYHPTAPHPEGKG
                     AARAIRAALGAAGVEPEEIRYVNSHGTGTPKNDPAETKATRLALGAAADTVAVSSTKS
                     MIGHLLGAAGAVECIVTVKALQEQVAPPTANYRERDPECDLDYVPNVARPLAMDVALS
                     NNFAFGGANASLVLGRGGLRRTPKSPERDRVVITGRSTLTSAGTDPEEVWRAFAEGRA
                     CTSVEDGVRIGRVALDPSPFLSPRERRRMDRLGIFSVISAKLALGDAGLTLTDENRER
                     IGAILGTGVGPMESLENFTRPVIEEGAAGANPAIFPNTVYNAAVGQVAMQVGTLGPAS
                     AVTAGHAAGASALCYGYDLLVSDQADAMLCLAADTLTDTVIGGYRDLGVLAKAEPEGR
                     DQGGYALAEAGVALVLERLSHARGRGAAILGEVLGCAITSDARGVGQYDPEGAGLEQA
                     MRLALERAGLEPSDVGAIWSGLDGHPGIDAAERAAINRVFGDVSEDAGAGGAGGDGSA
                     PRLFAPKLLFGEPVGAGAPLSAVLALAAWQRRPEGVPSDRPALINSCSLGGTNFSIVL
                     AAHV"
BASE COUNT          405 a         1121 c         1037 g          437 t
ORIGIN      
        1 ggggccgtcc aatccaccac ggcgccggcg tagatccact tcgtgctctc cagcccaacc
       61 agcatgatcc ggtcgcccgg cttcacctgg ccgtgccgcc aggcgtgatc gaggaagagc
      121 gggaccgccg cgcagccggt cgcgccggat tgggccaggt tgtcgaagat cttgccgtcc
      181 agcgcggccc agtccgccgt ggtcaggccg ccctcctcca tgctgtcgag catccagccg
      241 acgttcccct cggggatcag gcagaggtcg atctcctgca cgccgacgcc gctccggcgt
      301 agcgtctcgc cgatcgcctg ggtgatcatg tatggcgtga acttgcccgc ttcgagcacg
      361 tccacccgca gatcgaccag ccgtcgggcc ttgagctgtt cggcgatcgg tgcgtgggtg
      421 ccgccgccga cggcgtggat gcccggcttc ttgccgccgc cgacgcaggc ctgggccgcc
      481 ccgacgatcc ccggcgcgcc ttcctcgccg gcccgcagca ccatcgcgcc ggcgccgtcc
      541 ccgaacatgt agagcggcat ccgctcgcgc atccgcaccg cgtccgggct cttacccagg
      601 tagaccggga tcaggaccgg cgagatcgtc tcgctgccga tcaccagtgc cgtcttgtag
      661 acgccgcgct ccaggtacat cttggcgatg tccagcccct ggacgacgcc cgcgccgccg
      721 gagcgcagct ccatcgtggc gcactgaccg atcccgaggc ggtcctgcac gaagttgacg
      781 accggcggca ggtgatagtc cggggtgccc gtcgccagga tcatcaggtc gatctcgccg
      841 gcatcgacgt tggccgactc cagcgccatc ctggccgccg tgtacgccat gtcggagttg
      901 ttttcgcggt gcgcgccggt gaacggatcg gtcatccaga agcgctgctg gatcgagatg
      961 ccctccatca cgtcgtcggg cagcggcccc accagccgct cgagggtcgc gttgtctatc
     1021 gggtcacccg gcaggtggcc gccggtgccg agaatctgga ctggtctcac ggctggtcct
     1081 ctcacgctct cgttcacgat ctatgtttca ggctcagacg tgcgcggcca gcacgatcga
     1141 gaagttcgtg ccgccgagcg aacagctgtt gatgagtgcc gggcggtcgg atgggacgcc
     1201 ttccggccgg cgctgccatg cagcgagggc cagcaccgcg ctgagcggcg cgcccgcgcc
     1261 gaccggctcg ccaaagagca gcttgggggc gaacaggcgc ggcgccgagc cgtccccgcc
     1321 cgctccaccg gccccggcat cctccgaaac gtcgccgaag acgcgattga tggccgcgcg
     1381 ctcggcggcg tcgatgcccg ggtgcccgtc cagcccggac cagatcgcgc ccacgtcgga
     1441 cggctccagc ccggcccgct ccagcgccag ccgcatcgcc tgctccaggc ccgcaccctc
     1501 ggggtcatac tggccgacgc cacgggcgtc cgaagtgatg gcgcagccaa gcacctcgcc
     1561 caggatggcc gcgccacggc cgcgcgcatg ggagaggcgt tccagtacca gggcgacgcc
     1621 ggcctcggcg agggcgtatc ctccctggtc ccggccctcc ggctcggcct tcgccagcac
     1681 gccgaggtcg cggtaccccc cgatcacggt gtccgtcagg gtgtcggcgg cgaggcagag
     1741 catcgcgtca gcctggtccg agacgagcag gtcgtagccg tagcagagcg ccgaggcgcc
     1801 ggcggcgtgc ccggctgtca ccgccgaggc cggcccgagc gtgccgacct gcatggcgac
     1861 ctgcccgacc gccgcgttgt agacggtgtt cgggaagatc gccgggttgg cgccggccgc
     1921 cccctcctcg atgaccgggc gggtgaagtt ctccaggctc tccatcggcc cgacgccggt
     1981 cccgaggatc gcgccgatcc gctcgcggtt ctcgtcggtc aaggtcaggc cagcgtcgcc
     2041 gagcgccagc tttgccgaga tgaccgagaa gatcccgagc cggtccatcc gccggcgctc
     2101 gcgcggcgag aggaacggcg acgggtcgag cgccacccgc ccgatgcgaa cgccgtcctc
     2161 gacgctggtg caggcgcgac cctcggcgaa cgcccgccag acttcctcgg ggtcggtgcc
     2221 ggcgctggtc agcgtcgagc ggccggtgat gaccacacga tcgcgctccg ggcttttcgg
     2281 ggtccggcgc aggccgcccc ggccgagcac cagcgaggcg ttggcccccc cgaacgcgaa
     2341 gttgttcgag agcgccacgt ccatcgcgag cggccgggcg acgttcggca cgtagtcaag
     2401 gtcgcactcc ggatcgcgct cccggtaatt tgccgtgggc ggcgcgacct gctcctgcaa
     2461 ggccttgacc gtcacgatgc actcgacggc gccagcggcc ccgagcaggt gcccgatcat
     2521 cgacttggtg ctgctgaccg cgaccgtgtc ggcggcggcc ccgagtgcca gccgggtcgc
     2581 cttcgtctcg gccgggtcgt tcttcggggt gccggtcccg tggctgttga cgtagcggat
     2641 ctcctccggc tcgacgccgg ccgcccccag cgcggcgcgg atcgcccggg ccgcgccctt
     2701 cccctcggga tggggcgcgg tcgggtggta gccgtccgcc gagagcgcgt agccgagcac
     2761 ctcggccagc accggcgccc cgatccgctc ggccacgtcc tcgcggacca gcaccagcat
     2821 cccgctgccc tcgccgagcg agagcccctg gcgatccttc gagtacgggg cagccggctc
     2881 cggcgagagc gactccaggc agttgaaacc ggcgaacagc acgtcggaga gggcgtcggc
     2941 gccaccggtc aggacggcgt cggcctggcc ggagcggatc agctcggcgg cgtagccgat
//
LOCUS       DASTZO010000307         4954 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_651580,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000307 DASTZO010000000
VERSION     DASTZO010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4954)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4954)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4954
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_651580"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VFU20_08620"
     CDS             <1..482
                     /locus_tag="VFU20_08620"
                     /inference="COORDINATES: protein motif:HMM:NF026018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEU5286551.1"
                     /translation="ADSAQKPADLLGKVYYPEVVRMDLSTKKPWKLMWRGTVLATVAG
                     VLTGLVLLVAGRWVVDALFGAEFLPAYPVLLVLIVAPFLAMVSFPLVPMLYALDRPDA
                     PLKARLIGTLLYFAVVAPLAWRFGVIGAAIAFVIGVAAMVAVQAVQLLREYRRVRAR"
     gene            479..1471
                     /locus_tag="VFU20_08625"
     CDS             479..1471
                     /locus_tag="VFU20_08625"
                     /inference="COORDINATES: protein motif:HMM:NF018017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ELM1/GtrOC1 family putative glycosyltransferase"
                     /protein_id="HEU5286552.1"
                     /translation="MSDPPRIWVLLGARVGDNNQLLALAEAFRLPFETRTMGYRRRWI
                     RWLRLFPTLPFLLDRRSRRTLGPPWPDLVIGIGRRSVAVARWIRWRNRGRTSIVRLGN
                     PRADPTLFDLVLTTPQYPVPQADNVVVLPLSMRRHREIVPQPGETAWLERLPRPHLLL
                     SLGGVTRFWTLANEVVADSAARLAERAAAAGGTLIVIGSPRTAPDTIEAVKAAVGGRP
                     NVATDPPVRFPVLLADADEHCVTADSVSMISEAVMTGKPVALVPVELDEEGQEELGRE
                     QGYSSEFRDLRRFWAELDRRGLAGTVDSPRSGTVEDPVAIAVAAVKRLLGDRVE"
     gene            complement(1451..2326)
                     /locus_tag="VFU20_08630"
     CDS             complement(1451..2326)
                     /locus_tag="VFU20_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286553.1"
                     /translation="MKRTVFIQANDRQMLGARISAHSYRRNARDPGAFDVRILDIRDH
                     PRLLQRGQTLLRGGHVREWDPDDLQSFTPLRFYPPQAMGFEGLAVVTDPDIFGVGDVG
                     ELFERDLAGKAIWAVARPGHNSQDDYIATSVMLLDCARLSHWQFDQELDDLFAHRFDY
                     IDWIELKREDRSTIGLLEPEWNDFDRLTPQTKLLHTTKRRTQPWKTGLPVDYTLRESG
                     WLDSVRRLRRRRYEQHPDRRQEALVYSLLADMLDTGEISKQELVAEMAANHVRHDSLE
                     LVERYRGWRLLDAVA"
     gene            complement(2340..3191)
                     /gene="kdsA"
                     /locus_tag="VFU20_08635"
     CDS             complement(2340..3191)
                     /gene="kdsA"
                     /locus_tag="VFU20_08635"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEU5286554.1"
                     /translation="MQLLGRTVGLDQPLFLIAGPCVVESEELQLSTAEALKRITDRLG
                     IMFIFKSSFDKANRSSGASYRGPGMDEGLRILAKVRDTLGVPVLTDVHDIPQIQPVAE
                     VVDVLQTPAFLARQTDFITAVASAGRPVNIKKAQFMAPADMKNVVDKARTAAKAAGGD
                     GDNILVCERGASFGYNNLVSDMRSLAIMRDTGCPVVFDATHSVQLPGGQGTSSGGQRE
                     HVPVLARAAVAAGVAGLFMETHPDPANAKSDGPNAWPLDRMERLLTTLVDLDRTVKEA
                     GFEEMSL"
     gene            complement(3201..4004)
                     /locus_tag="VFU20_08640"
     CDS             complement(3201..4004)
                     /locus_tag="VFU20_08640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HEU5286555.1"
                     /translation="MSFLDALDERYRLVLCDIWGCVHDGVALYPGAAERLQRWRGEGR
                     TVVLITNAPRTAEAVERQLGRIGLPRDAWDGIATSGEAGIAALKELGRAVGFLGTAAD
                     REILEGKSVAIADGDDFTDLACTGLEEGRPHADQYAGQLADWVGRGVVMHCLNPDRLV
                     IRGGVPEACAGALADVYEALGGRVAWYGKPHKAIYEHALHLADDPAPDAVLAIGDGLH
                     TDMLGAARMGFDSVFVTGGIHAGEPFPPDFARQEGLGDWHPVAIVDSLG"
     gene            complement(4001..4798)
                     /locus_tag="VFU20_08645"
     CDS             complement(4001..4798)
                     /locus_tag="VFU20_08645"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011241263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manno-octulosonate cytidylyltransferase"
                     /protein_id="HEU5286556.1"
                     /translation="MNVAILIPARFASSRYPGKPLVELRGATGLAKPLIQRSYEAALR
                     VAGARSVHVVTDDLRIADQAAAFGASVIMTSAECRNGTERCAEALQHLDSPDLVVNLQ
                     GDALLTPPGFVEALVEHMRGDPDAQVATPAMRLRSSEVRALQAEEAAGRVGGTTVVAG
                     AGGRALYFSKRLIPHLPDEALDGELSPVRLHVGVYAYRPNALAAYAATPVSELETLEG
                     LEQLRFLTAGIPIAVVEVATPPFALRELNNPEDVAPIEAALAAAGLE"
BASE COUNT          688 a         1719 c         1776 g          771 t
ORIGIN      
        1 tcgccgacag cgcccagaag cccgccgacc tgctcggcaa ggtctattac cccgaggtcg
       61 tgcggatgga cctttcgacc aagaagccgt ggaagctgat gtggcgcggg acggtgctgg
      121 cgaccgtggc gggcgtgctc accgggctgg tgctgctggt ggccggccgc tgggtggtcg
      181 acgccttgtt cggggccgag ttcctgcccg cctacccggt gctgctggtg ctgatcgtcg
      241 cgccgttcct ggcgatggtc agcttcccgc tggtgccgat gctctacgcg ctcgaccggc
      301 ccgacgcgcc cctgaaggcg cggctgatcg gcacattgct ctatttcgcg gtggtcgctc
      361 cgctcgcgtg gcggttcggg gtgatcggcg ccgcgatcgc cttcgtcatc ggggtcgcgg
      421 cgatggtcgc ggtccaggcc gtccagctgc tgcgcgagta tcggcgggtc cgcgcccggt
      481 gagcgacccg ccccgaatct gggtgctgct cggcgcgcgg gtcggcgaca acaaccagct
      541 gctggcgctc gccgaagcat ttcggctgcc gttcgagacc cgcacgatgg gctaccggcg
      601 gcgctggatc cgctggcttc ggctgttccc gaccctgccc ttcctgctcg accgccgcag
      661 ccgccggacg ctcggcccgc cgtggccgga cctggtgatc ggtatcggcc ggcgaagcgt
      721 cgcggtggcg cgctggatcc ggtggcgcaa ccgcgggcgg accagcatcg tgcgcctcgg
      781 caatccgcgc gccgacccga cgctgttcga cctggttctg acgaccccgc aatatccggt
      841 gccgcaggcc gacaatgtcg tcgtgctgcc gctgtcgatg cgccgccacc gcgagatcgt
      901 gccgcaaccg ggcgagaccg cgtggctcga gcgcctgccc cgtccccatt tgctgttgtc
      961 gctgggcggc gtcacgcgct tctggacgct ggcgaacgag gtcgtcgccg acagtgcggc
     1021 acggcttgcc gagcgggcgg cggcggccgg agggacgctg atcgtcatcg gcagcccgcg
     1081 caccgccccg gacacgattg aggcggtgaa agcggcggtg ggcggccgcc cgaacgtcgc
     1141 gaccgacccg ccggtgcgct tcccggtcct gctcgccgac gccgacgagc attgcgtcac
     1201 cgccgacagc gtgtcgatga tctccgaggc ggtgatgacc ggcaagccgg tcgccctggt
     1261 gccggtcgag ctcgacgagg aaggccaaga ggagctcggc cgcgagcagg gctattcgtc
     1321 ggagttccgg gacctgcggc gattctgggc cgagctcgac cgccgcggcc tcgccgggac
     1381 cgtcgattcg ccgcgcagcg ggacggtcga ggacccggtc gcgatcgcgg tagcggcggt
     1441 gaagcgcctg ctaggcgacc gcgtcgagta gccgccagcc gcggtagcgc tcgaccagct
     1501 ccagtgaatc gtggcgaacg tggttggcgg ccatctccgc gaccagctcc tgcttcgaga
     1561 tctcgccggt atcgagcatg tcggcgagca gcgaatagac cagcgcctcc tggcgccggt
     1621 cgggatgctg ctcgtagcgg cggcggcgca gtcggcggac cgagtcgagc cagccgcttt
     1681 cgcgcagcgt gtagtcgacc ggaaggccgg tcttccacgg ctgggtgcgc cgcttggtgg
     1741 tgtgcagcag cttggtttgg ggagtcaggc ggtcgaaatc gttccactcg ggctcgagca
     1801 ggccgatggt cgagcggtcc tcgcgcttca attcgatcca gtcgatgtag tcgaagcggt
     1861 gggcgaacag atcgtccaat tcctggtcga actgccagtg gctgagcctc gcgcagtcga
     1921 gcagcatcac cgaggtcgcg atataatcgt cttggctgtt gtggccgggg cgcgccaccg
     1981 cccagatcgc cttgccggcc agatcgcgct cgaacagctc gccgacgtcg ccgaccccga
     2041 aaatgtccgg gtcggtgacc accgcgagcc cctcgaagcc catcgcctgc ggcgggtaga
     2101 agcgtagcgg ggtgaagctc tgcaaatcgt cggggtccca ttcgcgcacg tgcccgccgc
     2161 gcaggagggt ctggccgcgc tgaagcagcc gcggatggtc gcggatgtcg aggatccgga
     2221 cgtcgaaggc gccgggatcg cgcgcattgc ggcggtagct gtgggcggaa atccgcgccc
     2281 cgagcatctg gcggtcgttg gcctggatga acaccgtccg cttcacggcc gcaactcgct
     2341 cacaggctca tctcctcgaa ccccgcctcc ttgaccgtgc ggtcgaggtc gacgagcgtc
     2401 gtcagcaggc gttccatccg gtcgagcggc caggcgttgg gcccgtccga cttggcgttg
     2461 gcgggatcgg gatgggtttc catgaacagc cccgcgaccc cggccgcgac cgccgcccgg
     2521 gcaagcaccg gcacatgctc gcgctggccg cccgagctgg tcccctgccc gcctggcaat
     2581 tgcaccgaat gggtcgcgtc gaacaccacc gggcagccgg tgtcgcgcat gatcgccagg
     2641 ctgcgcatgt cggagacgag gttgttgtag ccgaacgagg cgccgcgctc gcagaccagg
     2701 atattgtcgc cgtcgccgcc cgcggccttc gcggcggtac gcgccttgtc gacgacgttc
     2761 ttcatgtcgg cgggcgccat gaactgcgcc ttcttgatgt tgaccggcct gccggcgctg
     2821 gcgaccgcgg tgatgaagtc ggtctggcgg gcgaggaagg ctggcgtctg gagcacgtcg
     2881 acgacctcgg cgaccggctg gatctgcgga atgtcgtgaa cgtcggtcag caccggcacg
     2941 ccgagcgtgt cgcggacctt ggcgaggatc cgcaggccct cgtccatgcc cgggccgcgg
     3001 tagctggcgc ccgacgagcg gttggccttg tcgaagctcg acttgaagat gaacattatg
     3061 ccgagcctgt cggtaatccg cttcagcgcc tcggcggtgc tgagctgcag ctcctcgctc
     3121 tcgaccacgc acgggccggc gatcaggaac aggggctggt cgagcccgac ggtgcggcca
     3181 agcaattgca tcggcgtgcc ttagccgagc gagtcgacaa tcgccaccgg atgccagtcg
     3241 ccgaggccct cttgccgggc gaaatcgggc gggaacggct cgccggcgtg gatcccgccg
     3301 gtcacgaaca cgctgtcgaa gcccatccgg gcggcgccga gcatgtcggt gtggagccca
     3361 tcgccgatcg ccagcaccgc gtcgggcgcc gggtcgtcgg cgaggtgcag cgcgtgctcg
     3421 tagatcgcct tgtgcggctt gccgtaccag gcgacccgac cgccgagcgc ctcgtagacg
     3481 tcggcgagcg ccccggcgca cgcctcggga accccgccgc ggatcaccag ccggtcgggg
     3541 ttgaggcagt gcatgacgac gccccgcccg acccagtcgg cgagctgacc ggcgtactgg
     3601 tcggcgtgcg gcctcccctc ttcgagcccg gtgcaggcga ggtcggtgaa atcgtcgccg
     3661 tcggcgatcg cgacgctttt gccctcgagg atttcgcggt cggcggcggt gccgaggaag
     3721 ccgaccgcac ggccgagctc cttcaaggcc gcgatccccg cctcgccgct ggtcgcgatc
     3781 ccgtcccacg cgtcgcgggg caggccgatc cggcccagct gccgctcgac cgcctcggcg
     3841 gttcgcggcg cgttggtgat cagcaccacc gtccgccctt cgccgcgcca gcgttgcagg
     3901 cgctcggcgg cgccgggata gagggcaacg ccgtcatgga cgcagcccca gatgtcgcac
     3961 agcaccagcc ggtagcgctc gtcgagcgcg tccagaaagc tcattcgagg cccgccgcgg
     4021 cgagcgccgc ctcgatcggc gcgacgtcct cggggttgtt gagctcgcgc agagcgaacg
     4081 gcggcgtcgc gacctcgacg acggcgatcg ggattccggc ggtgaggaag cgcaattgct
     4141 cgagcccctc gagcgtctcg agctcgctga ccggagtcgc cgcataggcc gccagagcgt
     4201 tgggccggta cgcgtagacg ccgacgtgaa gccgcaccgg cgacagctcg ccgtcgagcg
     4261 cttcgtcggg caggtgcggt atcagccgct tggagaaata gagcgcgcgg ccgccggcgc
     4321 cggcgacgac ggtggtcccg ccgacccgcc ccgccgcttc ctcggcctgg agcgcgcgta
     4381 cctccgagct gcgcaggcgc atcgccgggg tcgcgacctg cgcgtcggga tcgccgcgca
     4441 tatgctcgac caaagcctcg acgaagccgg gcggggtcag cagcgcatcg ccttgcaaat
     4501 tgacgaccaa atcgggcgag tcgaggtgct gcagcgcctc ggcgcaacgt tcggtgccgt
     4561 tgcggcattc ggccgacgtc atgatcacgc tggcgccgaa cgcggccgcc tggtcggcga
     4621 tcctgagatc gtcggtgacg acatgcaccg agcgtgcccc ggcgactcgc agcgccgcct
     4681 cgtagctgcg ctggatcagc ggcttggcga gcccggtcgc tccgcgcagc tcgaccagcg
     4741 gcttgccggg atagcgcgac gaagcgaacc gcgccgggat caggatcgcg acattcacgc
     4801 gacggtcacc gggcggccgg gcagtcgtgg atgtgcagca ctcccaccgg gcgctgcccg
     4861 ccctcgcctt gctcgacgac gaacagcacc gtgatccggt gctcctcgaa cagggtcagc
     4921 gcctcgtcga ccagagcgtc cgggccaacg gtct
//
LOCUS       DASTZO010000308         3791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_287041,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000308 DASTZO010000000
VERSION     DASTZO010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3791
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287041"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VFU20_08650"
     CDS             complement(<1..591)
                     /locus_tag="VFU20_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5286557.1"
                     /translation="MAAPILSYEGLGLVQGEGWLLRGIDVHVGARDRLALIGRNGVGK
                     TTLLKCLAGLIETDEGRRTVVPGTRVVLLEQDPAMSGSETLSDWVLQGPDGPSRHEAE
                     AIADQLGVDLERPVATASGGERRRAAIARALAQKPNLLLLDEPTNHLDLAALEWLERF
                     LKDWDGTLIVISHDRYFLDKVTNRTLEIANGKLDGDY"
     gene            727..1023
                     /locus_tag="VFU20_08655"
     CDS             727..1023
                     /locus_tag="VFU20_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286558.1"
                     /translation="MSLYRTLLAALIGAGLAASPALADPPRDQDRAFEAAREGRSMPL
                     PKIERKVMPFMDGADYLGPEFNGETYRLKFMRGGRVIWVDVDAATGRILRRSRP"
     gene            1122..1787
                     /locus_tag="VFU20_08660"
     CDS             1122..1787
                     /locus_tag="VFU20_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5286559.1"
                     /translation="MRVLIVEDEPNLGRQLRSTLEGAGYAVDLATDGEDGHYLGSTES
                     YDAVILDLGLPEVDGLTVLDRWRKEGKRMPVLVLTARDSWSDKVAGLDAGADDYLAKP
                     FQTEELIARLRALIRRASGNASSELTAGDIRLDTRSGKVTKAGEPVKLTAQEYKLLSY
                     LMHHKGKVVSRTELIEHIYDQDFDRDSNTIEVFVTRIRKKLGADVITTIRGLGYSLED
                     NPA"
     gene            1874..3181
                     /locus_tag="VFU20_08665"
     CDS             1874..3181
                     /locus_tag="VFU20_08665"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5286560.1"
                     /translation="MTGVAALWIIVLLLLGGFALDRVLTRSIERNFDDQLEYVLNAMI
                     AASEIGPDGEVRFNRPPADQRFIEPYSGVYFQISGAGADTFPSRSLWDRRLRVASRHN
                     DVSVHTYDSREFAGEPLRIAERDAILPGSEVRWRFQVAQSREIIDLQIRELRSTLTWS
                     FAVLGAGLLVLAGLQTVYGLWPLRRVQREVAMIRSGEKTRIGQEFPSEIRPLTEEINQ
                     LLAHSEAQAEEARRHAGNLAHALKTPLTVITNAATARTPDLADTVCREALVMRRQVDH
                     HLARARAIGRRATAQARAVVWESLEAVQRALDRLYENVTVDIAGDRDAQVRVERQDLD
                     EMIGNLVENAAKYGGGRVFVTVEPCGGMVDVLVEDDGPGIPPADRGSLFSRGARLDTT
                     GKPGTGLGLAIVRDVAEIYGGRITLEESEDLGGLLARLTLPAG"
     gene            3241..>3791
                     /locus_tag="VFU20_08670"
     CDS             3241..>3791
                     /locus_tag="VFU20_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5286561.1"
                     /translation="MTATVHPLRSPREPSLAAMMSLVAADMNGVNAVILERMQSKVAL
                     IPELAGHLIAGGGKRMRPMLTLACANLLGYSGTRHHKLAAAVEFIHTATLLHDDVVDG
                     SGTRRGKRTANLIWGNPASVLVGDFLFSRAFELMVEDGSLKVLKILSQASAVIAEGEV
                     EQLTAQRRIDTGEEQYLHIIGAKT"
BASE COUNT          618 a         1360 c         1253 g          560 t
ORIGIN      
        1 ataatcgccg tcgagcttac cgttggcgat ctcgagcgtg cggttggtca ccttatcgag
       61 gaaatagcga tcgtgcgaga tcacgatcag cgtgccatcc caatccttca gaaagcgctc
      121 cagccattcc agtgccgcca ggtcgagatg gttggtcggc tcgtcgagca gcagcaggtt
      181 cggcttctgc gccagcgccc gggcgatcgc cgcgcgccgc cgctcgccgc cgctggcggt
      241 cgcgaccggc cgctcgaggt cgaccccgag ctggtcggcg atcgcctcgg cctcgtgccg
      301 cgaagggcca tccgggccct gcagaaccca atccgacagt gtttcggacc cgctcatcgc
      361 cgggtcctgc tcgagcagca ccaccctggt ccccgggacc acggtgcgcc gcccctcgtc
      421 ggtctcgatc aggccggcga ggcatttcag gagagtcgtc ttgccgacgc cgttgcgccc
      481 gatcagcgcc agccggtcgc gggcgccgac gtggacatcg atgccgcgca acagccagcc
      541 ttcgccctgg accaggccga ggccttcgta ggagaggatc ggagccgcca tcggacgcgc
      601 cctctaggca atcgcgggcg cgcggtcacc ctgtgagagg cccgaaactg gctgaatagg
      661 tccgttcgca accattcata gcgcattcaa tggcttgtgt tagaacatcg gccaagagaa
      721 tcgcccgtga gcctgtaccg cacccttctt gccgcgctga tcggcgccgg ccttgccgcc
      781 agccccgcgc tggccgaccc gccgcgcgac caggaccgcg cgttcgaggc ggcccgcgaa
      841 ggccgctcga tgccgctgcc caagatcgag cgcaaggtga tgccgttcat ggacggcgcc
      901 gactatctcg ggcccgagtt caacggcgag acctatcggc tgaagttcat gcgcggcggc
      961 cgggtgatct gggtcgacgt cgacgccgcc accggccgca tcctgcgccg ctcgcggccg
     1021 taagccgcaa tatcttcccg tgcggcttgt cgttcccgtg ccgcaaccga tccgctatga
     1081 tacgggttga ccgcgggtaa cggctaggag ccccaagaga catgcgcgtt ttgatcgtcg
     1141 aggacgaacc gaatctcggg cgccagctgc gctcgacgct cgaaggcgcc ggctatgccg
     1201 tcgacctcgc caccgacggc gaggacggcc attatctggg ctcgaccgaa agctatgacg
     1261 cggtgatcct cgacctcggc ctgccggagg tcgacgggct gaccgtgctc gaccgctggc
     1321 gcaaggaagg caagcgcatg ccggtgctgg tgctgaccgc ccgcgacagc tggtccgaca
     1381 aggtcgccgg gctcgatgcc ggcgccgacg actatctcgc caagccgttc cagaccgagg
     1441 aattgatcgc ccggcttcgg gcgctgatcc gccgcgccag cggcaatgcc tcctcggagc
     1501 tgaccgccgg cgacatccgc ctcgacaccc gctcgggcaa ggtcaccaag gccggcgagc
     1561 cggtcaagct gaccgcgcag gagtacaaat tgctgtcgta cctgatgcac cacaagggca
     1621 aggtggtcag ccggaccgag ctcatcgagc atatctacga ccaggacttc gaccgcgatt
     1681 ccaacaccat cgaggtgttc gtcacgcgta tccgcaagaa gctcggagcg gacgtgatca
     1741 ccaccatccg cgggctcggc tacagcctcg aggacaatcc cgcttgaacg acagcgccgc
     1801 cccagggacg gcggcggtaa cggcggcggc tgcggggtcc agccgccgcg gcatcgggtc
     1861 gctgacccgg cggatgaccg gcgtcgcggc gctgtggatc atcgtcctgc tgctgctcgg
     1921 cggcttcgcg ctcgaccggg tgctgacaag gtcgatcgag cgcaacttcg acgaccagct
     1981 agaatatgtg ctcaacgcga tgatcgccgc gtccgagatc gggcccgacg gcgaggtccg
     2041 cttcaaccgc ccgccggccg accagcgctt catcgagccc tattccggag tctatttcca
     2101 gatcagcggc gccggcgccg acaccttccc gtcgcgctcg ctgtgggacc gccggctgcg
     2161 cgtcgcttcc cgccacaacg acgtcagcgt ccacacctac gacagccgcg agttcgccgg
     2221 cgagccgctg cgcatcgccg agcgcgacgc gatcctgccc ggatccgagg tgcgctggcg
     2281 attccaggtc gcccagtcgc gcgagatcat cgacctgcag atccgcgagc ttcggtcgac
     2341 cctgacctgg agcttcgcgg tgctcggcgc cgggctgctg gtgctcgccg ggctgcagac
     2401 ggtctacggg ctgtggccgc tgcgccgggt gcagcgcgag gtcgccatga tccggtcggg
     2461 cgagaaaacc cgcatcggcc aggaattccc gtccgaaatc cgcccgctga ccgaggagat
     2521 caaccagctg ctcgcgcaca gcgaggcgca ggccgaggaa gcgcggcggc atgcgggcaa
     2581 cctcgcccac gcgcttaaga cgccgctgac ggtgatcacc aacgccgcga ccgcccggac
     2641 ccccgacctt gccgataccg tctgccgcga ggcgctggtg atgcggcgcc aggtcgacca
     2701 ccacctcgcc cgggcccgcg ccatcggccg ccgggcgacg gcgcaggcgc gcgcggtggt
     2761 gtgggagagc ctcgaggccg tccagcgcgc gctcgaccgc ttgtacgaga acgtcacggt
     2821 cgacatcgcc ggcgaccgcg acgcccaggt gcgcgtcgag cggcaggacc tcgacgagat
     2881 gatcggcaat ttggtcgaga atgccgccaa atacggcggc ggccgggtgt tcgtgaccgt
     2941 cgagccgtgc ggcggaatgg tcgacgtcct ggtcgaggac gacgggcccg gcattcctcc
     3001 ggccgaccgc ggctcgctgt tcagccgcgg ggcccggctc gataccaccg gcaagcccgg
     3061 caccggcctc ggcctcgcca tcgtccgcga cgtcgccgag atctacggcg gccggatcac
     3121 gctcgaggaa agcgaggacc tcggcggcct gctcgcccgg ctcaccctgc ccgcgggcta
     3181 aggccgcaga tcagggtcgc aggctaggaa agcgtcggcc cagcggctag tgcgggcgcc
     3241 gtgaccgcta ccgtccatcc gctgcgttcg ccgcgcgagc cgtcgctcgc cgcgatgatg
     3301 agcctggtcg cggccgacat gaacggcgtc aacgccgtca tcctcgaacg catgcagagc
     3361 aaggtggccc tgatccccga gctcgccggc catctgatcg ccggcggcgg caagcggatg
     3421 cggccgatgc tgaccctcgc ctgcgcgaac ctgctcggct attcgggcac ccgccaccac
     3481 aagctcgccg cggcggtcga gttcatccac accgcgaccc tgctccacga cgacgtcgtc
     3541 gacggctccg gcacccgccg gggcaagcgc accgccaacc tcatctgggg caatccggcc
     3601 agcgtcctgg tcggcgattt cctgttcagc cgggcgttcg agctgatggt cgaggacggc
     3661 agcctgaagg tgctgaaaat cctcagccag gcatcggccg tgattgccga gggagaagtc
     3721 gagcagctaa ccgcccagcg ccgcatcgac accggcgagg agcaatatct ccacatcatc
     3781 ggcgccaaga c
//
LOCUS       DASTZO010000309         3811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_887386,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000309 DASTZO010000000
VERSION     DASTZO010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887386"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1016
                     /locus_tag="VFU20_08675"
     CDS             <1..1016
                     /locus_tag="VFU20_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5286562.1"
                     /translation="AQGAVAVAAASAMSFVSPALVRPDNIVAVAIGWLVVLTAINALG
                     VRAAGGFSVVTVMIKLLPLLAVVWIFGERGAIGGAFEPLAPAPINLTNLAAATALTFF
                     AFTGFEAATTPVDKVRDPERTLPRALIGGVTFTVLLYLVAGTAIQMLLPADVVSVSPA
                     PFADALTSRFGSGAAAFAALTIAVAAVGCLNGLILATGELGYSMALRGDMPKAMGWTR
                     GVNTPVVSQAVGSAVSALVLLANNSKATASLYTFIILLGTASVVIVYLAATLGAWMHS
                     PRLRQRSILALALLFIAFATYGIGMEPVMWSLVLLAIGIAIRWIMHRLDSQAGSSPAV
                     AET"
     gene            complement(961..2112)
                     /locus_tag="VFU20_08680"
     CDS             complement(961..2112)
                     /locus_tag="VFU20_08680"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HEU5286563.1"
                     /translation="MSLPGWLYHDPEFFEAEKNAFLRAAPQVVCHESEIAQAGEWRSL
                     EYLGTSVIVIRGDDGEVRAFHNVCRHRGSRLVDGEAGCAKVLTCPYHAWSYARDGRLV
                     GVPHRNEYPGLEPDQLGLVPVALEQWRGFLFVTLEPGAASVAEIMAPYEAEVAAYRFE
                     ELRPIGRVTLRPRPLNWKTIADNYSDGLHIPVGHPGLTRLFGRNYRIEAREHVDRMEG
                     DLGDKPSANPSERAYQQYLPAVDHLPASHRRKWIYYKLFPNVAFDIYPDQVDFMQFLP
                     VSATETMIREISYALPDAFIPETRRREMRAARYLNWRINRRVNAEDTQLIARVQAGMQ
                     SPAYVPGPLGTSEVCLRSFAQKLRRLIPQARLTSPPPPGWSRLANRAGA"
     gene            complement(2216..2938)
                     /locus_tag="VFU20_08685"
     CDS             complement(2216..2938)
                     /locus_tag="VFU20_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286564.1"
                     /translation="MRGAVPSRAIRADAAGDQVAEGPPAPEAAAEPQNTYCERLNDFA
                     TPAECAYFDTIWQKLATGTAGVRFPSDLTRGESGVVSLVIALGGSGAGPSAEDLLGQR
                     PDDEFKLKVARRMAAQLQGVGFEIDPGGLQHRDFHMGQGVRWDWRVTAVKARRHALTL
                     STYVVVRGPDGTQSETLLRSVERDLPVRVTLTQRFSDMVDWVVGISTLTKTLFGALGV
                     ILVALIAFRQRLGELKAAFVGK"
     gene            3433..>3811
                     /gene="rpsF"
                     /locus_tag="VFU20_08690"
     CDS             3433..>3811
                     /gene="rpsF"
                     /locus_tag="VFU20_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU5286565.1"
                     /translation="MPLYEHVFLARQDLAQAQVDALAENATKILTDNGGTVVKTETWG
                     LRSLAYRIAKNRKAHYVMLDVEAPPAALAELERQTNINEDVIRFLTLRVDEHETAPSA
                     MMRRGERDRERGPRGDDRGDRGGF"
BASE COUNT          576 a         1320 c         1272 g          643 t
ORIGIN      
        1 ccgcgcaagg cgcggtcgca gtcgcggccg cctcggccat gtcgttcgtc tcgccggcgc
       61 tcgtccgccc cgataatatc gtcgcggttg cgatcggctg gctggtggtg ctgaccgcaa
      121 tcaacgcctt gggagtccgc gccgcaggcg gcttttcggt cgtcaccgtc atgatcaaat
      181 tgctcccgct gctcgccgtg gtgtggattt tcggtgagcg cggcgcgatc ggcggagcct
      241 tcgaaccgct tgcccccgct cccatcaacc tcaccaatct ggcagccgcg acggcgctga
      301 ccttcttcgc ttttaccggc tttgaagccg cgacgacccc ggtcgacaag gtccgcgatc
      361 cggaacggac cctgccccgt gcactgattg gcggcgtgac ctttaccgtg ctcctctatc
      421 ttgtggctgg aaccgccatc cagatgctgc ttccggccga tgtcgtctcc gtctcgccgg
      481 cgcccttcgc ggatgcgctg acctcgcgct tcggcagcgg ggcggcagcc tttgccgcgc
      541 tgaccatcgc cgtcgccgcc gtcggctgcc tcaacggcct catcctcgcc accggtgagc
      601 ttggctattc gatggcgctg cgcggcgaca tgccaaaggc aatgggctgg acccgggggg
      661 tcaacacccc ggtcgtgtcc caagccgtcg gcagcgctgt cagcgccctt gtcctgctcg
      721 ccaacaacag caaggccacc gccagcctct atactttcat catcctgctc gggacggcgt
      781 cggtggtgat cgtctatctc gccgcgacgc tgggcgcctg gatgcatagt ccgaggctcc
      841 gccagcggtc catccttgcg ctggccctgc tgttcatcgc ctttgcgacc tacggcatcg
      901 gcatggagcc ggtgatgtgg agcctggtgc tgctcgcgat cgggattgcc atccgctgga
      961 tcatgcaccg gctcgattcg caagccggct ccagcccggc ggtggcggag acgtaagccg
     1021 cgcctgcgga atcagccgcc gcaatttctg cgcgaagctg cgcaggcaca cctcgctggt
     1081 gcccagcggc ccgggcacat aggccgggct ctgcattccc gcctgcactc gggcgatcag
     1141 ctgcgtgtcc tcggcgttga cccgccggtt gatgcgccag ttcaaatagc gcgccgcacg
     1201 catctcgcgc cgccttgttt ccggaataaa ggcatcgggc agcgcatagc tgatctcgcg
     1261 gatcatcgtc tcggtcgccg acaccggcag gaactgcatg aaatcgacct ggtcggggta
     1321 gatgtcgaag gcgacgttgg ggaacagctt gtagtaaatc cacttgcgcc ggtgcgaggc
     1381 cggcaaatgg tccaccgccg gcagatactg ctggtaggcg cgctcggatg ggttggcgga
     1441 cggcttgtcg ccgagatcgc cttccatccg gtcgacatgc tcgcgcgcct cgatcctgta
     1501 gttcctgccg aacaggcgcg tcaggcccgg gtgcccgacc gggatgtgca gcccgtcgga
     1561 ataattgtcg gcgatggtct tccagttgag cggccgcggc cgaagcgtca cccggccaat
     1621 cggccgcagc tcctcgaagc ggtacgccgc gacctcggcc tcgtacggcg ccataatctc
     1681 cgcgacgctc gcagcgccgg gctcgagcgt cacgaacagg aagccgcgcc actgctcgag
     1741 cgcgaccgga accagcccca attggtccgg ctcgagcccg ggatattcat tgcggtgcgg
     1801 gaccccgacc aaccgcccgt cgcgcgcata gctccacgcg tgataggggc aggtcagcac
     1861 cttggcgcag ccggcctcgc cgtcgaccag ccgcgagccg cggtgccggc agacattatg
     1921 gaaagcccgc acctcgccgt cgtcgccgcg gatcacgatc acgcttgtcc cgaggtattc
     1981 gaggctgcgc cattcgccgg cttgcgcgat ctcgctctcg tggcagacca cctgcggcgc
     2041 ggcgcgcaag aacgcgtttt tctcggcctc gaagaattcg gggtcgtggt acagccagcc
     2101 gggcaggctc atcccgtcga gcgggtcggc cgccggctcg gccaggtgca gtttggcgct
     2161 cacccggccc gaatgccatt tgtccgggcc gtcggcaact gcgccggcgg gccggctatt
     2221 tgccgacgaa cgcggctttc agctcgccga gacgctggcg gaaggcgatc agggccacca
     2281 ggatcactcc cagcgcaccg aacagcgtct tggtcagggt cgagatcccg accacccagt
     2341 ccaccatgtc ggaaaagcgc tgggtcaggg tgacccgaac cggaaggtcc ctttccaccg
     2401 acctgagcaa cgtctcgctc tgcgttccgt cggggcccct gacgaccacg taagtgctca
     2461 aagtcagcgc atgccgccgc gccttgacgg ccgtcacccg ccagtcccag cgcacccctt
     2521 gccccatgtg gaagtccctg tgctgaaggc cgcccgggtc gatttcgaag cccacgccct
     2581 gcagctgcgc cgccatccgc cgagcgacct tgagcttgaa ctcgtcgtcg ggccgctggc
     2641 cgagaaggtc ttcggcggaa ggccctgccc cgcttcctcc caacgcgatg accaggctga
     2701 cgactccgct ttcgccacgc gtgaggtcgc tggggaaccg gactccggcg gtaccggtcg
     2761 ccagcttctg ccagatcgtg tcgaaatagg cgcactcggc gggcgtcgca aaatcgttca
     2821 ggcgctcgca gtaggtgttt tgcggctcgg ccgccgcttc gggcgctggt gggccctccg
     2881 cgacctggtc tcccgccgca tcggcacgaa tcgcgcgaga gggaaccgcc ccgcgcatcg
     2941 gccggggagg ctcctgcacg ctgggcggcg gcgccgacag ttgggacgcc tgcacgatcg
     3001 gtggcggtgg cggcggcggc gggggtggtg ggggcagtgg cggcggcggc ggcggtgcag
     3061 gcggtggagg cggcggcggg ggcggcgact cgacggtcgc gggcgccgtg cacgccgact
     3121 gcagacagag cggcaacgcc atcaccgcga tgccgagacg aaggcggctc gccttcacgc
     3181 tcggccggaa cgcgctgatg gggatgggac aggcatggcg cgatgtgcca cggcgcgccg
     3241 gctacaagca aggaattgcg ttgcggccgc cgatcgtgaa gcccgacggc cttgcttttc
     3301 gcgcgaggtt tggccatagg cgcggcgcgt ggggcggacc ttccggttcg cctcttgccg
     3361 tttttgagac gacagccgga ggggcgtccg cactgggcgg cgtcagcaat cggccaacga
     3421 agagacagag acatgccgct gtacgagcat gttttcctcg cgcgtcagga cctcgcccag
     3481 gcccaggtcg acgcgctcgc ggaaaacgcc accaagattt tgaccgacaa tggcgggacg
     3541 gtcgtcaaga ccgagacctg gggcctccgg agcctcgcct accggatcgc caagaaccgc
     3601 aaggcccatt acgtgatgct cgacgtcgag gcgccgccgg ccgcgctcgc cgagctcgag
     3661 cgccagacca acatcaacga ggacgtgatc cgcttcctca ccctgcgcgt cgacgagcat
     3721 gagactgctc cgtcggcaat gatgcgccgt ggcgagcgtg accgtgagcg tggcccgcgc
     3781 ggcgacgacc gtggcgaccg cggtggtttc a
//
LOCUS       DASTZO010000310         4785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1017922,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000310 DASTZO010000000
VERSION     DASTZO010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1017922"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /locus_tag="VFU20_08695"
     CDS             complement(<1..1166)
                     /locus_tag="VFU20_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5286566.1"
                     /translation="MPSFARELEQTLHNALGEASRRRHEYATLEHLLIALVDDEHASK
                     VMSACGVSRDELRASVKQYLDNELGALVADNGTDPTPTSGFQRVVQRAILHVQSSGRD
                     EVTGANVLVALFSERESYAVFFLQQQDMSRLDAVTYISHGVGKGDTPAEAREVGAAEE
                     KSDKGEGKKESALKQFTVDLNEKARQGKVDPLIGRMPEVDRTVQILCRRSKNNPLYVG
                     DPGVGKTAIAEGLARKIVEGDVPDVLKPAVIYALDMGALLAGTRYRGDFEERLKSVVG
                     ELEKLEHAILFIDEIHTVIGAGATSGGAMDASNLLKPALSSGTIRCIGSTTYKEFRNH
                     FEKDRALLRRFQKIDVNEPTVEDTIKIIAGLRAAFELHHNVRYTPDAIKSAVELS"
     gene            complement(1269..1466)
                     /locus_tag="VFU20_08700"
     CDS             complement(1269..1466)
                     /locus_tag="VFU20_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HEU5286567.1"
                     /translation="MDLDDLFPDKPKDPLTELGRQDLDPLSIEELGERIEALRAEIAR
                     VEAHIERVKRDRSAAEELFKR"
     gene            1517..2548
                     /locus_tag="VFU20_08705"
     CDS             1517..2548
                     /locus_tag="VFU20_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEU5286568.1"
                     /translation="MAGSATPSSMRAVAAPQPDGAEELGVTERPVPRAGPGEVLIEVA
                     AAGVNRPDILQRRGLYPPPPGAPDILGLEVAGTVVEAGAGAEELVGKRVCALVAGGGY
                     AEYCLAPAGTCLPVPDALTIAEAAAMPETLFTVWVNLFERGFAADGDAVLVHGGTSGI
                     GTMAIALGKLFGLEVIVTCGTDSKCARAVELGAAAAINYRTQDFVAEAKRLTDGRGVA
                     VVLDMVGGDYLPRNLAALADEGRHVSIAFQRGAKAELSIPEIMSRRLTLTGSTLRARP
                     VEFKTMVADEIAKTVWPYVEGGRLKPVIDRIIHLDRAAEAHARMEAGEHVGKIVLAVS
                     PLALQAPQD"
     gene            2631..3377
                     /locus_tag="VFU20_08710"
     CDS             2631..3377
                     /locus_tag="VFU20_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1013 domain-containing protein"
                     /protein_id="HEU5286569.1"
                     /translation="MPHATASWLVENSSLTFEQIAEFCGLHILEVQAIADDTAATKLT
                     GRDPVRAGELTHEEIEKGQADPDYRLKMHKEPDPVRRTRGPRYTPVSKRQDKPDGIAW
                     IIRNHPEVSDGQIGKLIGTTRTTIAAIRDRTHWNMANIQPKDPVTLGLTSQRELDAAI
                     AKAAKAAGTPAYVDTTLDSDRLALIDELRAERDQHARDAEQALAAAEAAEAAEAAGET
                     TGEGAEAGKVGELAAGEIVPGIRDPFKRQG"
     gene            3426..3683
                     /locus_tag="VFU20_08715"
     CDS             3426..3683
                     /locus_tag="VFU20_08715"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU5286570.1"
                     /translation="MDASPAPDEQTGELSFEAALARLEEIVRLLERGEAPLDQSIELY
                     QEGDRLKRHCEARLKAAQARIEQIALGADGKPSGVTPFDGS"
     gene            3673..4587
                     /locus_tag="VFU20_08720"
     CDS             3673..4587
                     /locus_tag="VFU20_08720"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011414043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HEU5286571.1"
                     /translation="MGVEIIDRSRAEFETESNRLSAEIDSFFVRLLENSGDARDRLYQ
                     AMRHAAVGGGKRLRPLLTSAAAQLFAIDPERALRVGAAIEAIHVYSLIHDDLPCMDND
                     DLRRGKPTVHKAFGEAEAVLAGDSLHALAFEILAHPATHEDPHVRSDLVFELAKAAGP
                     AGMAGGQMMDLVAEGEELDIAAITRLQQLKTGALIEYSVEAACIMVRLAEAARTPYRG
                     YARNIGLAFQIADDLIDHSGDEATAGKPLGRDADAGKATFVSLLGEERARQQAHMLVD
                     QAIEHLGDHGEEADLLRAIARFAVERDH"
     gene            4591..>4785
                     /locus_tag="VFU20_08725"
     CDS             4591..>4785
                     /locus_tag="VFU20_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyltransferase/cytidyltransferase family
                     protein"
                     /protein_id="HEU5286572.1"
                     /translation="MKTAVYPGTFDPVTLGHLDIIRRGAHLVEKLVIGVTTNPSKEPM
                     FAVADRLAMVKREIIGIPGNI"
BASE COUNT          760 a         1650 c         1672 g          703 t
ORIGIN      
        1 gacaactcga ccgccgactt gatcgcatcc ggggtgtagc ggacattgtg gtgaagttcg
       61 aaggcggcgc gaaggccggc gatgatcttg atcgtgtcct cgaccgtcgg ctcgttgacg
      121 tcgatcttct ggaaccggcg aagcagcgcc cggtcctttt cgaaatggtt gcggaattcc
      181 ttgtaggtgg tcgagccgat gcagcgaatg gtgcccgacg acagcgccgg cttcaaaagg
      241 ttcgacgcgt ccatcgcccc gccgctggtc gcgccggcgc cgatcacggt gtggatctcg
      301 tcgatgaaca ggatcgcgtg ctcgagcttc tcgagctcgc cgaccaccga cttcagccgc
      361 tcctcgaaat cgccgcgata gcgggtgccg gcgagcaaag cgcccatgtc gagcgcgtag
      421 atcaccgccg gcttgaggac gtcggggacg tcgccctcga cgatcttgcg cgccaggcct
      481 tcggcgatcg cggtcttccc gacgccgggg tcgccgacgt agagcgggtt gttcttcgac
      541 cggcggcaga ggatctggac ggtgcggtcg acctcgggca tccggccgat cagcgggtcg
      601 accttgccct ggcgcgcctt ctcgttgagg tcgacggtga actgcttgag cgcgctttcc
      661 ttcttgccct cgcccttgtc gcttttctcc tcggcggcgc cgacctcgcg ggcctcggcg
      721 ggcgtgtcgc ccttgccgac cccgtggctg atgtaggtga ccgcgtcgag ccggctcatg
      781 tcctgctgct ggaggaagaa caccgcatag ctctcgcgct cggagaacag ggcgaccagc
      841 acgttggctc cggtgacctc gtcgcggccc gacgactgga cgtgcaggat cgcgcgctgg
      901 acgacccgct ggaagccgct ggtcggggtc gggtcggtgc cgttgtcggc gaccagggcg
      961 ccgagctcgt tgtcgagata ttgtttgacg ctcgcgcgca gctcgtcgcg gctgaccccg
     1021 caggcgctca tcaccttcga cgcgtgctcg tcgtcgacca gggcgatcag cagatgctcg
     1081 agggtcgcat attcgtggcg gcggcggctc gcctccccaa gcgcattgtg cagggtctgt
     1141 tcgagctcgc gggcgaaact gggcatcggg acgaaaactc cgtgcccgct cgggacaagc
     1201 gggctgattc ccaatgtcat acggagcggc gccgcaatca aggggcagcg gcgcgcttta
     1261 tccccggttc agcgcttgaa cagctcctcg gccgccgagc ggtcgcgttt gacccgctcg
     1321 atatgggcct cgactcgggc gatttcggcc ctgagcgcct cgattcgctc gcccagctcc
     1381 tcgatcgaca ggggatcgag gtcctggcgg ccaagctcgg tcagcgggtc cttgggcttg
     1441 tcggggaaca ggtcgtcgag gtccatcgcc cgcaaatcgt tgacccgcct gggcgcggtg
     1501 tcaataagct gcggccatgg cgggctcggc gactccatct tcgatgcggg cggtcgccgc
     1561 gccgcagccg gacggcgccg aagagctcgg ggtgaccgag cggccggtgc cccgggccgg
     1621 gcccggcgag gtcctgatcg aggtcgccgc ggccggggtc aaccgccccg acatcctgca
     1681 gcggcgcggc ctctacccgc cgccgcccgg ggccccggac atcctcgggc tcgaagtcgc
     1741 cgggacggtg gtcgaggcgg gcgccggcgc cgaggagctg gtcggcaagc gcgtctgcgc
     1801 cctggtcgcg ggcggcggct atgccgaata ttgcctcgcc ccggccggga cctgcctgcc
     1861 ggtccccgac gcgctgacca tcgccgaagc cgccgcgatg cccgaaaccc tgttcaccgt
     1921 ctgggtcaat ctgttcgaac gcggcttcgc ggccgacggc gatgcggtgc tggtccacgg
     1981 cggcaccagc ggcatcggca cgatggccat cgcgctcggc aaattgttcg ggctcgaggt
     2041 gatcgtcacc tgcgggaccg actcgaaatg cgcccgggcg gtggagctgg gcgccgcggc
     2101 ggcgatcaac taccggaccc aggacttcgt cgccgaggcg aagcggctga ccgacgggcg
     2161 cggggtcgcg gtggtgctcg acatggtcgg cggcgattat ctgccgcgca acctcgccgc
     2221 attggccgac gaaggccgcc acgtgtcgat cgccttccag cgcggggcga aggcggagct
     2281 gtcgatcccc gagatcatga gccgccgcct gaccctgacc ggctcgacgc tccgtgcgcg
     2341 gccggtcgag ttcaagacca tggtcgccga cgagatcgcc aagacggtgt ggccctatgt
     2401 cgagggcggg cgcctgaagc cggtaatcga ccgaatcatc cacctcgacc gcgccgccga
     2461 agcccatgcg cgcatggagg ccggcgagca tgtcggcaag atcgtgctgg cggtgagtcc
     2521 acttgccctg caggcgccgc aggactaaac ccccgccggc aacggccgct ccgggaaggg
     2581 gcggccctat tcatttgtaa tggagtggaa ataccgtggc gcagcccctc atgccgcatg
     2641 cgaccgcctc gtggctggtc gagaacagtt cgctgacctt cgagcagatc gccgagttct
     2701 gcgggctcca tatcctcgag gtgcaggcga tcgccgacga cacggcggcg accaagctga
     2761 ccgggcgcga cccggtgcgc gccggcgagc tgacccatga ggagatcgag aagggccagg
     2821 ccgaccccga ttaccggctg aagatgcaca aggagcccga tccggtgcgc cgcacccgcg
     2881 ggccgcgcta cacgccggtc agcaagcgcc aggacaagcc cgacgggatc gcctggatca
     2941 tccgcaacca cccggaggtc agcgacggcc agatcggcaa gctgatcggc acgacgcgca
     3001 cgaccatcgc cgcgatccgc gaccgcaccc actggaacat ggccaacatc cagcccaagg
     3061 acccggtgac gctcggcctg accagccagc gcgagctcga cgcggcgatc gccaaggcgg
     3121 ccaaggcggc gggcaccccg gcctatgtcg acaccaccct ggacagcgac cgcctggcgc
     3181 tgatcgacga gctgcgcgcc gagcgcgacc agcacgcgcg cgacgccgag caggcgctcg
     3241 ccgcggccga ggcggccgag gcggccgaag cggccggcga gaccacgggc gagggcgccg
     3301 aagccggaaa ggtgggcgag ctggctgccg gcgagatcgt cccgggcatc cgcgacccgt
     3361 tcaagcgcca gggctgaggc cgcgcaaact tgattcggga aggcgagcgg gtcaaagctg
     3421 cggccatgga cgcgtccccg gcccccgacg agcagaccgg cgagctgagc ttcgaggcgg
     3481 cgctcgcccg gctcgaggag atcgtccgcc tgctcgagcg cggcgaggcg ccgctcgacc
     3541 agtcgatcga gctctaccag gaaggcgacc ggctgaagcg ccactgcgag gcgcggctga
     3601 aagccgccca ggcgcgcatc gagcagatcg ccctgggcgc cgacggcaag ccttcgggcg
     3661 tgaccccgtt cgatgggagt tgagatcatc gaccgctcgc gggcggagtt cgagacggaa
     3721 tccaaccgcc tttccgccga aatcgacagc tttttcgtcc gcttgctcga aaacagcggc
     3781 gacgcccgcg accggctcta ccaggcgatg cgccacgccg cggtcggcgg cggcaagcgg
     3841 ctccggccgc tgctgaccag cgccgccgcg caattgttcg ccatcgaccc cgagcgcgcc
     3901 ctgcgggtcg gagcggcgat cgaggcgatc cacgtctatt cgctgatcca cgacgacctg
     3961 ccgtgcatgg acaacgacga cctgaggcgc ggcaagccga ccgtccacaa ggcgttcggc
     4021 gaggccgagg cggtgctggc cggcgacagc ctgcacgcgc tggcgttcga gatcctcgcc
     4081 cacccggcga cccacgagga cccgcacgtc cgctccgacc tggtgttcga gctggcgaag
     4141 gccgcggggc cggcggggat ggccgggggg cagatgatgg acctggtcgc cgagggcgag
     4201 gagctggaca tcgccgcgat cacccgcctg cagcaattga agaccggcgc gctgatcgaa
     4261 tattcggtcg aggcggcgtg catcatggtc cggctcgccg aggccgcgcg caccccctac
     4321 cgcggctatg cgcgcaacat cggcctcgcc ttccagattg cggacgatct catcgaccac
     4381 agcggcgacg aagcgaccgc gggcaagccg ctcggccgcg acgccgacgc gggcaaggcg
     4441 accttcgtgt cgctgctcgg cgaggaacgg gcgcgccagc aggcgcacat gctggtcgat
     4501 caggcgatcg agcatctggg cgatcatggc gaggaggccg acctcctccg cgccatcgcg
     4561 cgcttcgcgg tcgaacggga tcattaggtg atgaaaaccg ccgtctatcc gggcacgttc
     4621 gatcccgtca ccttggggca cctcgacatc atccggcgcg gtgcgcacct cgtcgagaaa
     4681 ctcgtcatcg gagtcaccac caacccgtcg aaggagccga tgttcgccgt cgccgaccgg
     4741 ctggcgatgg tgaagcgcga gatcatcggc attcccggca acatc
//
LOCUS       DASTZO010000311         7793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_71622,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000311 DASTZO010000000
VERSION     DASTZO010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7793
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71622"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..828)
                     /locus_tag="VFU20_08730"
     CDS             complement(<1..828)
                     /locus_tag="VFU20_08730"
                     /inference="COORDINATES: protein motif:HMM:NF014790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5286573.1"
                     /translation="MTEHLAWPFFEPRHRELATKLEKWCGEKLGVEHPEDVDAACRSL
                     VPELGDAGFLKLCVADGDKRPDVRSLAIARETLARHSGLADFAFAMQGLGSGSISLSG
                     NDEQKRRWLPKVASGDAIAAFAMTEPECGSDAASIAMTARRDGDAWVLDGEKTYISNG
                     GIADFYVTFARSGEHGVRGLSAFLVPVDAPGLSVAERIEVIAPHPLARLKYEGVRGEL
                     IGAEGDGFGIAMRTLNLFRVTVGAAALGFARRALDEALAFASWRKLGGGTLADNAVTQ
                     "
     gene            complement(821..1630)
                     /locus_tag="VFU20_08735"
     CDS             complement(821..1630)
                     /locus_tag="VFU20_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010127568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEU5286574.1"
                     /translation="MSFDPETFAPRHFLWNFHGGVATVTLDRPERKNPLTFDSYAELR
                     DTFRSLAATPQVKCVILTGAGSNFCSGGDVHEIIGPLTKMGLDGLLKFTRMTGDLVRA
                     MRACPQPIVAAVEGVCAGAGAILAMASDIRLAAPNAKTAFLFTRVGLSGADMGACAIL
                     PRIIGHGRAAELLFTGRTMSAEEGLAWGFYSRICDDVQAEARAFARTLADGPSLAHAV
                     TKRQLDAEWAEPVAIALDMEAEAQAALMQTNDFKRAYEAFAAKRAPEFKGD"
     gene            complement(1627..2397)
                     /locus_tag="VFU20_08740"
     CDS             complement(1627..2397)
                     /locus_tag="VFU20_08740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5286575.1"
                     /translation="MQSNTWLQGRHALVTGGGTGIGASAATHLHSAGAKVSLLGRRLE
                     PLERTAAAVAGTVHPCDVTDRAAIERAYAEARAANGPIEMLVVNAGIAESAPFAKMTR
                     DSWDRIIAVNLTAAFDCAQAALPDLLASDNGRLVFVASVASLRGVPYAAHYAASKHGL
                     LGLMRSMAAEFAKSNLTVNAVCPGYVDTPMTDQSVARVSQITGRSEGEARSAITNMNA
                     SGRLVHPDAVGTMILTLCLPQSRDINGAAVTIDGGTSA"
     gene            2479..3588
                     /locus_tag="VFU20_08745"
     CDS             2479..3588
                     /locus_tag="VFU20_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HEU5286576.1"
                     /translation="MKTRAAVAFEAKQPLEVVELDLEGPKAGEVLVEIMATGICHTDA
                     YTLDGLDSEGIFPSILGHEGAGVVREVGSGVTTVKPGDHVIPLYISECRQCKACLSRK
                     TNLCTAVRATQGKGVMPDGTSRFSYNGRTIHHYMGCSTFSNFTVLPEIAVAKIRDDAP
                     FDKACYIGCGVTTGVGAVINTAKVEPGSNVVVFGLGGIGLNVVQGARMVGANMIVGVD
                     INPGKEEWGRKFGMTHFVNPKEVEGDLVKHLIELTDGGADYSFDCTGNTDVMRAALEC
                     CHRGWGTSVVIGVAEAGKEIATRPFQLVTGRVWKGTAFGGARGRTDVPKIVDWYMDGR
                     IAIDPMITHTLTLEEINKGFELMHAGESIRAVVVY"
     gene            3588..4427
                     /gene="fghA"
                     /locus_tag="VFU20_08750"
     CDS             3588..4427
                     /gene="fghA"
                     /locus_tag="VFU20_08750"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007712068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046292 - formaldehyde metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HEU5286577.1"
                     /translation="MELLSEARSHGGRQLVCRHASAATSTGMTFSIFLPPQAEGGARL
                     PVVWYLSGLTCTHANVTEKGEYRAACAELGLMFVAPDTSPRGEGVPDDLAGGWDFGLG
                     AGFYVDATQEPYAQHYRMWSYVTDELPALVAAEFPADMERQAIMGHSMGGHGALTVAL
                     RHPERLRSVSAFSPIVAPSQVPWGQKALRGYLGDDRNAWRRHDAVALIEDGARVPEML
                     VDVGDADPFLEQELRPELLEEACAAAGIALTLNRRPGYDHSYYFISTFMADHLRWHAQ
                     RLG"
     gene            complement(4431..6089)
                     /locus_tag="VFU20_08755"
     CDS             complement(4431..6089)
                     /locus_tag="VFU20_08755"
                     /inference="COORDINATES: protein motif:HMM:NF006903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006847 - plasma membrane acetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation acetate symporter"
                     /protein_id="HEU5286578.1"
                     /translation="MLPAAALADTRLATERGTNWPAVLVFAAFVVVTLGITRWAARRT
                     RTTADFYAAGGGITGFQNGLAIAGDYMSAASFLGISAQIFADGYDGLIYSTGFLVGWP
                     IILFLLAERFRNLGRYTFADVASFRFGQTPVRLFAAFSTLAVVIFYLIAQMVGAGQLI
                     TLLFGLPYGFAVGIVGVLMTLYVMFGGMIATTWVQIIKAVLLLGCATVMAFAVLASFD
                     FSPDALFARAIEVKSGLAAAAGAPPAEAAARGASIMAPGNFIKDPISAISFGMALMFG
                     TAGLPHILMRFITVPDAKAARKSVLWATGWIGYFYLLTFIIGFGAIVMVASNPAFLDP
                     GGGLRGGGNMAAVHLASAVGGNLFLGFVSAVAFATILAVVAGLTLAGASAVSHDIYAS
                     VLRHGTAKSEDELRVSRITVLVLAAVAILLGILFEKQNVAFMVSLAFALAASGNFPVL
                     LLSILWGGSTTRGVLWGGLVGVLTALVLTVLSPAVWVTTFGFAEAVFPYSSPTIFSMP
                     LAFLAIFIVSKLDRSDRAALDRAGYLEQRVRSETGIGASGAARH"
     gene            complement(6134..6484)
                     /locus_tag="VFU20_08760"
     CDS             complement(6134..6484)
                     /locus_tag="VFU20_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017977396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HEU5286579.1"
                     /translation="MTDTTQLDSPPADTIDARPLLEVARDPRYLQLVRERSRFSWILT
                     AIMLVVFFGYILLIAFAPELLARRIGGGVTTLGIPLGIAVILCGILLTAIYVHRANRR
                     FDPIIREITQRPGP"
     gene            complement(6526..6601)
                     /locus_tag="VFU20_08765"
     tRNA            complement(6526..6601)
                     /locus_tag="VFU20_08765"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6565..6567),aa:Glu,seq:ttc)
     gene            complement(6641..>7793)
                     /locus_tag="VFU20_08770"
     CDS             complement(6641..>7793)
                     /locus_tag="VFU20_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006959183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HEU5286580.1"
                     /translation="LAAVGMAFLTAAAVNRELRGRGHFGGRPEPKLLDLLDLVALGTV
                     ADVARLKTLNRAFVTQGLKVMAERRNIGLSALAEAARLVKPPCCRDLGFALGPRINAG
                     GRVGKSDLGVRLLTSTDPEEARAIAAELDRLNEERRAIEMLVSEQAEAQAREQADAPL
                     ILVMSAGWHQGVIGIVAGRLKERFGRPAIVVAECEDGTGKGSGRSIGGVDLGAAVLAA
                     KESGLLVAGGGHAMAAGLTLPAGGLEPLRAFLHERLAADVEASLGGRALLLDALLAPG
                     GVAAALCDALDVAGPYGAGWPAPRVAAGPARLLRTGVVGDGHVRGLACGDDGKSFKWI
                     AFRSAATELGQALLASPGDRKWWLAGTIKRDEWNGGNAAEMHLEDAALA"
BASE COUNT         1276 a         2662 c         2756 g         1099 t
ORIGIN      
        1 ctgggtcacc gcattgtcgg cgagcgtgcc gccgccaagc ttgcgccacg acgcgaacgc
       61 cagcgcctcg tccagcgccc ggcgcgcgaa gccgagcgcg gcggctccga ccgtcacccg
      121 gaacaggttc aaggtccgca tcgcgatccc gaagccgtcg ccttccgcgc cgatcaactc
      181 gccgcgcacg ccttcgtatt tcaatcgcgc gagcggatgc ggggcgatca cctcgatgcg
      241 ctcggcgacg ctcagccctg gcgcgtcgac cggtaccagg aacgccgaca gtccgcgcac
      301 tccgtgttcg ccgctgcggg cgaacgtcac gtagaaatcg gcgatgccgc cgttggagat
      361 ataggtcttc tcgccgtcca ggacccaggc atcgccgtcg cgccgagcgg tcatggcgat
      421 gctcgccgcg tcggagccgc actcgggctc ggtcatcgcg aaggcggcga tggcgtcgcc
      481 cgacgcgacc ttgggcagcc agcggcgctt ctgctcgtcg ttcccggaaa ggctgatcga
      541 gcccgacccg aggccctgca tcgcgaacgc gaaatcggcg aggccggaat ggcgggcgag
      601 cgtctcgcgg gcgatcgcga ggctgcggac gtcgggccgc ttgtcgccgt cggcgacgca
      661 cagcttgagg aagcccgcgt cgcccagttc gggcaccagc gaccggcacg ccgcgtcgac
      721 atcctcggga tgctcgacgc cgagcttctc gccgcaccat ttttcgagct tcgtcgccaa
      781 ctcgcggtgg cgcggctcga agaacggcca ggcgagatgc tcagtcaccc ttgaactccg
      841 gagcccgctt ggccgcgaag gcttcgtagg ctcgcttgaa gtcgttggtc tgcatcagcg
      901 ccgcctgcgc ctcggcttcc atgtcgagcg cgatggcgac cggttccgcc cactcggcgt
      961 cgagctggcg cttggtcacg gcgtgggcga gcgaggggcc gtcggcgagg gtgcgcgcga
     1021 acgctcgggc ttcggcctgc acatcgtcgc agatgcggct gtagaagccc cacgccagcc
     1081 cttcctcggc gctcatggtg cggccggtga acagcagttc ggcggcgcgg ccgtggccga
     1141 tgatccgcgg caggatcgcg caggcgccca tgtcggcgcc cgacaggccg acgcgcgtga
     1201 acaggaacgc ggtcttggcg ttcggcgcgg cgaggcggat gtcgctggcc atcgccagga
     1261 tcgccccggc gccggcgcag acgccctcga ccgcggcgac gatcggctgc gggcaggcgc
     1321 gcatcgcccg caccaggtcg ccggtcatgc gggtgaactt gagcaggccg tcgaggccca
     1381 tcttggtcag cgggccgatg atctcgtgga cgtcgccgcc cgagcagaaa ttgctgccgg
     1441 cgccggtcag gatcacgcac ttgacctgcg gagtcgcggc cagcgaccgg aacgtgtcgc
     1501 gaagctcggc atagctgtcg aaggtcagcg ggttcttgcg ctcgggccgg tcgagggtga
     1561 cggtcgcaac gccgccgtgg aaattccaca ggaaatgcct gggtgcgaac gtctcggggt
     1621 cgaagctcac gcgctggtcc cgccgtcgat ggtcaccgcg gcgccgttga tgtcgcggct
     1681 ctgcggcagg cacagggtca ggatcatcgt cccgaccgcg tcggggtgga ccagccggcc
     1741 gctggcgttc atgttggtga tcgccgagcg cgcttcgccc tcgctccggc cggtgatctg
     1801 cgacacgcgc gcgaccgact ggtcggtcat cggcgtgtcg acatagccgg ggcagacggc
     1861 gttgacggtc aggttcgact tggcgaactc ggcggccatc gagcgcatca gcccgagcag
     1921 cccgtgcttc gaggccgcat aatgggcggc gtagggcact ccgcgcaggc tggcgaccga
     1981 ggcgacgaac accagccgtc cgttgtcgct cgccagcagg tcgggcagag ccgcctgggc
     2041 acagtcgaac gcggcggtca ggttgaccgc gatgatccgg tcccagctgt cgcgggtcat
     2101 cttcgcgaac ggcgcgctct cggcgatgcc ggcgttgacg accagcatct cgatcggccc
     2161 gtttgccgcc cgggcttcgg cataagcgcg ctcgatcgcc gcccggtcgg tgacgtcgca
     2221 cgggtggacg gtgccggcga cggccgccgc ggtgcgctcg agcggctcga gccgtcgccc
     2281 gagcagcgag accttcgcgc ccgccgagtg gaggtgggtg gcggccgaag cgccgatgcc
     2341 ggtgccgccg ccggtgacca gcgcatggcg gccttgaagc caggtgttcg actgcatggc
     2401 ttgccctctt gagatcagca ttggccgaag gccgggatgg tggcaaggcg gcgccaagac
     2461 gaacagggga gtcgcaccat gaagacccgc gccgccgtcg cgttcgaagc caagcagcca
     2521 ttggaggtgg tcgagctcga ccttgagggc cccaaggccg gcgaagtgct cgtcgagatc
     2581 atggcgaccg gcatctgcca caccgacgcc tatacattgg acggcctcga cagcgagggc
     2641 atcttcccgt cgatcctcgg ccacgaaggc gccggcgtgg tgcgcgaggt cggttcgggc
     2701 gtcaccacgg tcaagcccgg cgaccatgtg atcccgctct acatttccga atgccgccag
     2761 tgcaaagcct gcctgtcgcg caagaccaat ctgtgcaccg cggtccgagc cacccagggc
     2821 aaaggcgtga tgcccgacgg cacgtcgcgc ttcagctaca acggccggac catccatcac
     2881 tacatgggct gctcgacctt ctcgaacttc accgtcctgc ccgagatcgc ggtcgccaag
     2941 atccgcgacg acgcgccgtt cgacaaggcc tgctacatcg gctgcggggt gaccaccggg
     3001 gtcggcgcgg tgatcaacac cgccaaggtc gagccgggat ccaatgtggt cgtgttcggc
     3061 ctgggcggaa tcggcctcaa cgtcgtccag ggcgcgcgga tggtcggcgc caacatgatc
     3121 gtcggggtcg acatcaaccc gggcaaggag gagtggggcc gcaagttcgg gatgacccac
     3181 ttcgtcaacc cgaaggaggt cgagggcgac cttgtcaagc acctcatcga gctgaccgac
     3241 ggcggcgccg actacagctt cgactgcacc ggcaacaccg acgtgatgcg cgccgcgctc
     3301 gaatgctgcc accgcggctg ggggaccagc gtcgtcatcg gggtcgccga ggccggtaag
     3361 gagatcgcca cgcggccgtt ccagctggtc accggccggg tgtggaaagg gaccgcgttc
     3421 ggcggcgccc gcggccgcac cgacgtgccg aagatcgtcg actggtacat ggacggccgg
     3481 atcgcgatcg acccgatgat cacccacacg ctgacgctcg aggagatcaa caagggcttc
     3541 gagctgatgc acgccggcga aagcatccgc gccgtcgtcg tctattgatg gaacttcttt
     3601 ccgaagcgag atcacacggc gggcggcagc tggtgtgccg ccacgcgtcg gccgcgacgt
     3661 cgaccggcat gaccttttcc atctttctgc cgccgcaggc cgagggcgga gcgaggcttc
     3721 cggtcgtctg gtacctgtcg ggcctcacct gcacccacgc caacgtcacc gagaaaggcg
     3781 agtatcgcgc cgcctgcgcc gagctcggcc tcatgttcgt cgcgcccgac accagcccgc
     3841 gcggcgaggg cgtcccggac gacctcgccg gcggctggga cttcggcctc ggcgccggat
     3901 tctacgtcga cgcgacccag gagccgtatg cgcagcacta ccggatgtgg tcctatgtga
     3961 ccgacgagct gccggcgctg gtcgccgccg agttcccggc ggacatggag cggcaggcga
     4021 tcatgggcca ctcgatgggc gggcacggcg ctctgacggt ggcgctgcgc catcccgagc
     4081 gcttgcgcag cgtttcggcc ttttcgccga tcgtcgcccc gagccaggtg ccgtgggggc
     4141 agaaggcgct gcgcggctat ctcggcgacg accggaacgc gtggcgcagg cacgatgcgg
     4201 tcgctttgat cgaggacggc gcgcgggtgc cggagatgct ggtcgacgtc ggcgatgccg
     4261 acccgttcct cgaacaggag ctgaggcccg aactgctcga ggaggcgtgc gccgccgcgg
     4321 gcatcgccct caccctcaac cgccgcccgg gctacgacca tagttactat ttcatctcga
     4381 ccttcatggc cgatcatctg cgctggcacg cgcagcggct gggctgaaga tcagtgccgc
     4441 gcggcgccgc ttgcgccgat cccggtttcg gagcggactc gctgctcgag atagccggcg
     4501 cggtcgagag ccgcgcggtc gctgcgatca agcttggaga cgatgaagat ggcgaggaaa
     4561 gcgagcggca tcgagaagat cgtcggcgag ctgtagggga acacggcttc ggcgaagcca
     4621 aaggtcgtca cccacaccgc cggggagagc acggtcagga ccagggcggt gagaactccg
     4681 accaggccgc cccacagcac tccgcgggtg gtcgagccgc cccacaggat cgaaagcaaa
     4741 aggaccggga aattgcccga cgcagcgagc gcgaacgcga ggctgaccat gaaagcgacg
     4801 ttctgctttt cgaacaatat gccgagcagg atggcgaccg ccgccaggac cagcaccgtg
     4861 attcgcgaca cgcgcagttc gtcttcggat ttcgccgtgc cgtggcgcag gaccgacgcg
     4921 tagatgtcgt ggctgaccgc cgacgctccg gcgagggtca atccggcgac gacggcaagg
     4981 atggtcgcga acgcgaccgc cgatacgaac cccaggaaca ggttgccgcc gaccgcggac
     5041 gccagatgga ccgcggccat gttgccgccg ccgcgaagcc cgccgcccgg gtcgaggaac
     5101 gcggggttgc tcgcgaccat gacgatggcg ccgaagccga tgatgaaggt gagcaggtag
     5161 aaataaccga tccagccggt cgcccacagc accgacttgc gcgccgcctt cgcatcggga
     5221 acggtgatga agcgcatcag gatgtggggc aggccggcgg tgccgaacat cagcgccatt
     5281 ccgaacgaga tcgccgagat ggggtccttg atgaaattgc cgggcgccat gatcgaggcg
     5341 ccgcgggccg ccgcttccgc cggcggcgct ccggccgcgg cggcaaggcc gctcttcacc
     5401 tcgatggccc gcgcgaacag cgcatccggc gagaaatcga agcttgccag gacggcgaaa
     5461 gccatcaccg tcgcgcagcc gagcagcaag acggccttga tgatctgcac ccaggtggtg
     5521 gcgatcatcc cgccgaacat cacatagagc gtcatcagaa caccgacgat gccgaccgca
     5581 aagccgtacg gtaggccgaa cagcagggta atcagctggc ccgcgccgac catctgggcg
     5641 atcaggtaga agatcacgac cgcgagcgtg ctgaaggccg cgaacagccg cacgggcgtc
     5701 tgcccgaagc ggaaggacgc aacgtcggcg aaggtgtagc ggccgaggtt gcggaaacgt
     5761 tcggccagca ggaacaggat gatcggccac ccgaccagga agccggtcga gtagatcagc
     5821 ccgtcatagc cgtcggcgaa gatctgggcg gagatgccga gaaacgaggc cgccgacata
     5881 tagtcgccgg cgattgcgag cccgttctgg aaccccgtga tcccgccgcc cgccgcgtag
     5941 aaatcggcgg tggtgcgggt ccttcgggcg gcccagcggg tgatccccag cgtgaccacg
     6001 acgaaggcag cgaagaccaa tacggccggc cagttggtgc cacgctccgt cgccagccgg
     6061 gtatcggcga gcgccgcggc cggcaggatg gcggcgagca ggaggaccgc gacccgcggc
     6121 gcaagcgagc tcgtcaaggc cccggccgct gcgtgatttc gcggatgatc gggtcgaacc
     6181 gccggttggc ccggtgcacg taaatcgcgg tgaggaggat gccgcacagg atcacggcga
     6241 tgcccagcgg gatgccgagg gtggtgaccc cgccgccgat gcgccgggcg agcagctcgg
     6301 gcgcgaaagc gatcagcagg atgtagccga agaagacgac cagcatgatc gcggtcagga
     6361 tccagctgaa ccgggagcgc tcccgaacca gctgcaggta gcgcgggtcg cgcgcgacct
     6421 cgagcagggg gcgcgcgtcg atcgtgtccg ccggcggtga atcgagctgg gtggtgtccg
     6481 tcatgccgcc acttagcgct ttaccatcgc ccgaccgtcc aaccgtggtg accccaacgg
     6541 gactcgaacc cgtgttttcg ccgtgaaagg gcgacgtcct ggaccgctag acgatggggc
     6601 cgtgaagcgg ctcgccggtt aggcgagcgc cggaagtggg tcaagcgagc gcggcgtcct
     6661 cgaggtgcat ttcggcggcg ttgccgccgt tccattcgtc gcgcttgatc gtgcccgcga
     6721 gccaccattt ccggtcgccg ggcgaggcga gcagcgcctg gccgagctcg gtcgcggcgc
     6781 tcctaaaggc gatccatttg aagctcttgc cgtcgtcgcc gcaggcgagg ccgcggacgt
     6841 ggccgtcgcc gacgacgccg gtccttagca gccgcgccgg gccggccgca acccgcggcg
     6901 cgggccagcc ggcgccgtag ggcccggcca catcgagcgc gtcgcacagc gccgccgcca
     6961 ccccgccggg tgcgagcaga gcgtcgagca gcagcgcccg cccgccgagc gacgcctcga
     7021 cgtcggcggc gagccgctcg tggaggaagg cgcgcaacgg ctccagcccg cccgctggga
     7081 gagtcagccc ggccgccatc gcatggcccc ctccggcgac cagcagcccg ctttccttcg
     7141 ccgcgagcac cgccgcgccg aggtcaaccc cgccgatcga gcggcccgag cccttgccgg
     7201 tgccgtcctc gcactcggcg acgacgatcg ccggccggcc gaaacgctcc ttcaggcgcc
     7261 cggcgacgat gccgatcacg ccctggtgcc agccggcgct catcaccagg atcagcgggg
     7321 cgtcggcctg ctcccgagcc tgcgcttcgg cctgctccga aaccagcatc tcgatcgccc
     7381 gccgctcctc gttgaggcgg tcgagctcgg cggcgatggc gcgggcttcc tcggggtcgg
     7441 tcgaggtcag cagccgcaca cccaagtccg acttgccgac ccggccgccg gcgttgatcc
     7501 tggggccgag cgcgaagccg aggtcgcggc agcacggcgg cttgaccagc cgagcggcct
     7561 cggcgagcgc cgacaagccg atgttccggc gctccgccat gaccttcagg ccctgggtga
     7621 cgaacgcgcg gttgagggtc ttgagacggg cgacgtcggc gacggtgccg agcgcgacga
     7681 ggtcgagcag gtcgaggagc ttcggttccg gccgcccgcc gaaatggccg cgcccgcgca
     7741 gctcgcggtt gacggcggcg gccgtcagga acgccatgcc cacggcggcg aga
//
LOCUS       DASTZO010000312         3641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1279256,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000312 DASTZO010000000
VERSION     DASTZO010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279256"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..135)
                     /locus_tag="VFU20_08775"
     CDS             complement(<1..135)
                     /locus_tag="VFU20_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007683704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HEU5286581.1"
                     /translation="MALSVKSGALIVTAELGAEDFAWLDALRRRHYPPERNRVPAHLT
                     L"
     gene            complement(120..683)
                     /locus_tag="VFU20_08780"
     CDS             complement(120..683)
                     /locus_tag="VFU20_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain containing protein"
                     /protein_id="HEU5286582.1"
                     /translation="METSLSWVATAATILAASITASNLGTRITGYGFVVFTLGSIAWL
                     GFGLMSGQPALVWTNAALTVLNLFGIWRWLGRQARIEDGSAAAAEASRATPGEALFPV
                     SLLARAAVRARDERLGACVDAMAGCRSGRIAYVMVSEGGIAGAGETLRRLPWSRLRVE
                     GDEVVTGLDPAQFCRLEPVPKDEWPSR"
     gene            complement(715..2655)
                     /locus_tag="VFU20_08785"
     CDS             complement(715..2655)
                     /locus_tag="VFU20_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEU5286583.1"
                     /translation="MNITATGGAEADAPNDVSHGHAHGPLHKLALGAVGIVYGDIGTS
                     PIYAFRETFAGHHTLSPDRLHVYGVLSLIFWSMMIIVTTKYLSVIMRADNKGEGGSLA
                     LLALINRSIGGEKRWTAGIVLLGVFATALFYGDSMITPAISVLSAVEGLTTVEASFQP
                     YVIPVAIGILVGLFAIQSRGTAKVGSMFGPVMVTYFVTLAVLGVVHIVDHPAVVLATL
                     NPFNAVNFYIQEPMRAFIAMGSVVLAVTGAEALYADMGHFGRRPIRVAWLYFVMPALL
                     LNYMGQAAMLMAQSPAEAVETVKNPFFYLAPDMLRLPLVLLATLATIIASQAVISGAF
                     SVTQQAIQLGFMPRLRITHTSERAAGQIYIPVINWALLVMVVLLVLTFGSSSNLAAAY
                     GIAVTGAMLIDGVLIAVVLLTMWKWNRVLAIGLLAVFFTIDLLYFSANLLKVPDGGWF
                     PLLIGAIAFTLLTTWAKGRKLMIDRMAEASLPMEIFIKSAAGSAARVPGTAVFMTSSG
                     SGVPHALLHNLKHNKVLHERIILLTVRIEDIPYVPEDKRLETRDYGSGFYRLKLRYGF
                     MEEIDVPAALGKAKDCGPACKMMDTSFFLARQTLIASARPGMAIWREKLFAWMLRNAE
                     SAMEFFKLPHNRVVELGSQLEI"
     gene            2851..>3641
                     /locus_tag="VFU20_08790"
     CDS             2851..>3641
                     /locus_tag="VFU20_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HEU5286584.1"
                     /translation="MRIGVPREIKNHEYRVGLTPASVAELVAAGHEAFVETRAGNGID
                     CPDAAYRKAGATILPDAAAVFAASDMIVKVKEPQPQEIALLEPRHILFTYLHLAADKP
                     QAEGLMQSGATCIAYETVTSRSGALPLLKPMSEVAGRMSIQVGAHYLEKEQGGRGVLL
                     GGVPGVAPARVAILGGGVAGVNAAQMAVGMRADVTIYDISNERLAELDMFFGSQIKTA
                     YASRSAIANAVEKAELVIGAVLVPGAAAPNLVTREMLKTMKRGSVL"
BASE COUNT          641 a         1215 c         1245 g          540 t
ORIGIN      
        1 cagggtcagg tgcgccggca cccggttgcg ctccggcggg tagtggcggc gtcggagcgc
       61 gtcgagccag gcgaaatcct cggcgccgag ctcggcggtg acgatcagcg cgccgctttt
      121 caccgagagg gccattcgtc cttgggcacc ggctcgagcc tgcagaattg cgccgggtca
      181 agtccggtga cgacctcgtc cccctcgacc cgcagcctgc tccacggcag ccgccgcaag
      241 gtctcgccgg cgccggcgat gccgccttcg gaaaccatca cataggcgat ccgcccgctc
      301 cggcagccgg ccatcgcatc gacgcacgcc ccgagccgtt cgtccctcgc tcggacggct
      361 gcacgcgcca ggagcgacac cgggaacagc gcttcgccgg gagtcgctcg gctggcctcg
      421 gcggcggcgg ccgagccatc ctcgatcctc gcctggcggc cgagccagcg ccagattccg
      481 aacaggttga ggacggtgag cgcggcgttg gtccacacca aagccggctg gccgctcatc
      541 aggccgaagc cgagccaggc gatcgagccc aaagtgaaga cgacgaagcc gtagccggtg
      601 atccgggtgc caaggttgga cgcggtgatc gaggcggcga ggatggtcgc ggcggtggcg
      661 acccacgaaa ggctggtttc catggccgcg caacccgtgg ccgcgcgcga aggttcaaat
      721 ctcgagctgg ctgccgagct cgacgacgcg gttgtgcggg agcttgaaga attccatcgc
      781 gctttcggcg ttgcgcagca tccaggcgaa caacttctcg cgccagatcg ccatgcccgg
      841 gcgcgccgag gcgatcaggg tctggcgggc gaggaagaag ctggtgtcca tcatcttgca
      901 cgccgggccg cagtccttgg ccttgccgag cgccgcgggg acgtcgatct cctccatgaa
      961 tccgtaacgc agcttcagcc ggtagaaacc cgatccgtag tcccgggtct ccaggcgctt
     1021 gtcctcgggg acgtagggga tgtcctcgat ccgcaccgtc agcaggatga tccgctcatg
     1081 cagcaccttg ttgtgcttca agttgtggag cagcgcgtgc ggcactccgc tgcccgagct
     1141 ggtcatgaac accgcggtgc ccgggacccg ggcggcgctc ccggcggccg acttgatgaa
     1201 gatctccatc ggcaggctcg cttccgccat ccggtcgatc atcagcttgc ggcccttcgc
     1261 ccaggtggtg agcaaggtga aggcgatcgc cccgatcagc agcgggaacc agccgccgtc
     1321 gggcaccttc aggaggttgg ccgagaaata gagcaggtcg atggtgaaga acacggccag
     1381 caggccgatc gccagcaccc ggttccactt ccacatcgtc agcaggacga cggcgatcag
     1441 caccccgtcg atcagcatcg cgccggtgac cgcgatcccg taggccgcgg cgaggttcga
     1501 cgacgatccg aaggtcagga cgagcagcac caccatcacc agcagcgccc agttgatcac
     1561 cgggatgtag atctgcccgg cggcgcgctc gctggtgtgg gtgatccgga ggcgcggcat
     1621 gaagccgagc tgaatcgcct gctgggtgac cgagaaggcg ccggaaatga ccgcctggct
     1681 ggcgatgatc gtcgccagcg tcgccagcag caccagcggc agccgaagca tgtcgggggc
     1741 gaggtagaag aacgggttct tgaccgtctc gaccgcctcg gccggcgact gggccatcag
     1801 catcgccgcc tgccccatgt agttcaagag cagcgccggc atgacgaaat agagccacgc
     1861 gacgcggatc ggccggcggc cgaaatgacc catgtcggcg tacagcgcct cggcgcccgt
     1921 gacggcgagg acgaccgagc ccatcgcgat gaacgcccgc atcggctcct ggatatagaa
     1981 attgaccgcg ttgaacgggt tgagggtcgc caggacgacc gccgggtgat cgacgatgtg
     2041 aaccaccccg agcaccgcca gggtgacgaa ataggtgacc atcaccggcc cgaacatcga
     2101 cccgaccttg gccgtgccgc gcgactggat cgcgaacagg ccgacgagga tgccgatggc
     2161 gaccgggatc acatagggct ggaagctcgc ctcgaccgtg gtcaggccct cgaccgccga
     2221 caggacagag atcgccgggg tgatcatcga atcgccgtag aacagggcgg tcgcgaacac
     2281 gccgagcagc acgatccccg cggtccagcg cttctcgccg ccgatcgagc ggttgatcag
     2341 ggccagcagc gccaggctgc cgccctcgcc cttgttgtcg gcgcgcatga tgaccgacag
     2401 atatttggtc gtgacgatga tcatcatcga ccagaagatc aggctcagga ccccgtagac
     2461 gtggagccgg tcgggcgaca gcgtgtggtg cccggcgaac gtctcgcgga acgcgtagat
     2521 cggcgaggtg ccgatgtcgc cgtagacgat tccgaccgct ccgagcgcca gcttgtgcag
     2581 cggaccatgt gcgtgcccat gggaaacgtc gttcggcgcg tccgcctcgg cgcctccggt
     2641 cgcagtaatg ttcattgcgt gaagttctgc ctcgccttcg acagcaaaag tgggcttccg
     2701 ccaatccgca tcgaacccgt ccccggcatc cccaggtgcc cgggcgcgcc gcactagcac
     2761 cgctgttccg gagccgcaat cttgcgggga caaggccggg acacgcgtct atagcgcggc
     2821 gcgaacgtca ttttgggagc ggtgaaaacg atgcggattg gggtgccgag ggaaatcaag
     2881 aaccacgaat atcgggtcgg cctgaccccc gcatcggtcg ccgagctggt cgccgccggg
     2941 catgaggcgt tcgtcgagac ccgggcgggc aacggcatcg attgccccga cgccgcctac
     3001 cgcaaggccg gcgcgaccat cctgccggat gccgccgccg tgttcgcggc gtccgacatg
     3061 atcgtcaagg tcaaggagcc gcagccgcag gagatcgcgc ttctcgagcc gcggcacata
     3121 ttgttcacct acctccacct cgccgccgac aagccgcagg ccgaggggct gatgcaatcg
     3181 ggcgcgacct gcatcgccta cgagacggta acgtcgcgct cgggcgccct gcccctctta
     3241 aagccgatga gcgaggtcgc cgggcggatg tcgatccagg tcggcgcgca ctacctcgag
     3301 aaagagcagg gcggccgcgg cgtgctgctc ggcggagtgc ccggggtcgc gccggcgcgg
     3361 gtcgcgatcc tcggcggcgg ggtcgccggg gtcaacgccg cgcagatggc ggtgggcatg
     3421 cgcgccgacg tcaccatcta cgacatttcc aacgagcggc tggccgagct cgacatgttc
     3481 ttcggcagcc agatcaagac cgcctacgcg tcgcgcagcg cgatcgccaa cgcggtcgag
     3541 aaggccgagc tggtgatcgg cgcggtgctg gtgcccggcg cggcggcgcc caatctggtg
     3601 acgcgcgaaa tgctcaagac gatgaagcgc ggttccgttc t
//
LOCUS       DASTZO010000313         6863 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_856120,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000313 DASTZO010000000
VERSION     DASTZO010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6863)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6863)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6863
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856120"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..1824
                     /locus_tag="VFU20_08795"
     CDS             31..1824
                     /locus_tag="VFU20_08795"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017502559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEU5286585.1"
                     /translation="MSSYADRLAALRQQLKEDRLDGFVVPLTDEHMSEYVGTYAQRLA
                     WLTGFEGSAGSAVVLPQEAAIFVDGRYTLQVRQQVSADQWSYQSVPETSITDWLKEHA
                     REGARIGYDPWLHSRDWVKRASEALSQRGASLVAVQRNPIDAVWEDRPEASNAHLVVH
                     PDQYAGKSAAEKRTEIADWLSKEGADAAVLSALDSIAWAFNVRGADVARTPVALAYAL
                     VHSDGTADLFVASEKVDAQVRQHLGNGVRLHERTEFEPALAALEGKTVAVDPERAVAA
                     IFEALDKAGARILAVRDPTVLPKAIKNPAEIAGHRCAQERDGAAIAKFLKWVEAEAPK
                     GELDELTASDRLESLRREAPELRDLSFDTISGAGPNGAIVHYRSSEKTNRKLEPGTLY
                     LVDSGGQYVDGTTDITRTVPIGEPTAEMRDRFTRVLKGHIAVASALFPKGTRGSQLDS
                     FARRPLWEAGLDYAHGTGHGVGSFLSVHEGPQRIAPVGSAQSGGDEPLRAGMILSNEP
                     GYYKSGEYGIRIENLVLVVDRQVAGAEKEMLGFETLTHAPIERRLIVREMLGDQELAW
                     LNAYHAEVLAKIGPRLEGADRAWLEAACAPL"
     gene            complement(1939..2088)
                     /locus_tag="VFU20_08800"
     CDS             complement(1939..2088)
                     /locus_tag="VFU20_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286586.1"
                     /translation="MADKKKEREERLAAALKENLKRRKAQAREMAQGVIPAEAGISGQ
                     EGAGD"
     gene            2179..2253
                     /locus_tag="VFU20_08805"
     tRNA            2179..2253
                     /locus_tag="VFU20_08805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2211..2213,aa:Thr,seq:ggt)
     gene            complement(2421..3185)
                     /locus_tag="VFU20_08810"
     CDS             complement(2421..3185)
                     /locus_tag="VFU20_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286587.1"
                     /translation="MFLRRIIENLRAQNWTAITIEFAIVIVGVFIGTQVSNWNQQRIE
                     KRETAQLLLELRPALQSSMDFFETAKDYYATSRAYSDTAFVGWRGDPKVSDEQFVIAA
                     YQASQVYRFGVNGANWASVFGGERMRDIDSVAVRRSLTNLMAFNYNQVDLAAVATPYR
                     EHVRQLIPEDIQDAIRAECSDKPIPERPLTIRLPATCDLDLPEARFAQAASALRARPE
                     LVGELRWHRAAVAAYLVVLGAVERQTRELQQAIDQS"
     gene            complement(3308..4141)
                     /locus_tag="VFU20_08815"
     CDS             complement(3308..4141)
                     /locus_tag="VFU20_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEU5286588.1"
                     /translation="MSALQQQEYDAAAIMGGLYGDGIIGLKGAFSPEWADALHADIMT
                     LFEEAKAVPGGALPRGPERWYVEVHPERVRGFTDIATHPWFVAVCEAVLGPDYTIVEV
                     GFDIPFPGAADQPWHRDFPAPEATLIGRRLNSLAFNLPAVDCTLEMGALEIALRTQWD
                     DITGARDDMFPGPELWDRYAKRMVRKLPQRGDMSARSALTIHRGTANRSDLARPVLVV
                     GVDAPDAVNTDHHDVQVTRGYLDRLPPRVRDHLACRVSDALEPVIQRHVIEGLLEPSY
                     G"
     gene            4226..5227
                     /locus_tag="VFU20_08820"
     CDS             4226..5227
                     /locus_tag="VFU20_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010161023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-eliminating lyase-related protein"
                     /protein_id="HEU5286589.1"
                     /translation="MRFFSDNAAAAHPAVLEAIAASNRLDTAYDGDEWSARLDAAFSD
                     LFETRVRALWVTTGTAANCLALAALCPPHGGIICHRDAHIENDEAGAPGFYTNGAKLI
                     LVDGPGAKVTPDAVSGAIGRIRKDVHQVQPRALSITNATEYGLAFSAGEVAALGELAR
                     AHQLGFHMDGARFANASAATGARPADVTWRAGIDALSFGFVKNGGLNAEALVLFKAEL
                     ADEILVRRKRAGHLLSKGRMLAAQLLALLDNDLWLANARAANAAAKALAEAAPDRLVH
                     LVEANELFLKMTADEAARLRSQGFDFYDWGTGEIRLVTSWDQQGEAVGKLAAAIRAL"
     gene            5224..6162
                     /locus_tag="VFU20_08825"
     CDS             5224..6162
                     /locus_tag="VFU20_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019370730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5286590.1"
                     /translation="MTAALAVGRRADLSVIVPFLIFTAIWGSTWIVIRDQLGAVPPQW
                     SITYRFLLATAAMAAIARWKGGGLTLPAGGIAAAAVLGFTQFVINFNAVYLAEQHITS
                     GLVATVFALLLIPNTLLGWVFLGQRPDARFVAGSMAAVAGIGLLFAHELGENPARSGA
                     ILAGIGLTLAGMIAASIANVFQAREEVRRYPLFTLLAWAMGLGALMDGALALLIAGPP
                     VVELRPGYWLGIVYLAVFASAIAFSLYLPVVRKIGPAKAAYSSVLVPVIAMGFSTALE
                     GYRWTPMAAAGAVLALGGMLGALSRSRSVVPAPDAA"
     gene            6175..>6863
                     /gene="pepN"
                     /locus_tag="VFU20_08830"
     CDS             6175..>6863
                     /gene="pepN"
                     /locus_tag="VFU20_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010406055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HEU5286591.1"
                     /translation="MDARTIPEEPRPPAHVAIRREDYRPPDWLVPEVALDFTLDPAAT
                     RVRSTLTVVRNGNHRRPLRLDGDELTPLRVAVDGGEARWSMDGPALVIELSSDSATVE
                     TEVEVHPAANSKLTGLYASGGGLYTQCEAEGFRRITFFPDRPDVLSRYRVRMDADSAA
                     FPVLLTNGKCVESGQGEGGRHWARWEDPFPKPCYLFALVAGDLKPNRDSFTTMSGRKV
                     DLAIWVREEDLP"
BASE COUNT         1072 a         2456 c         2256 g         1079 t
ORIGIN      
        1 ggactagctc caattcaaag gagctaatcc atgtcatcct atgctgaccg ccttgcagcc
       61 ctccgccaac agctgaaaga ggatcgtctc gacggcttcg tcgtcccgct gaccgacgag
      121 catatgagcg aatatgtcgg cacctacgcc cagcggctcg cctggctgac cggcttcgag
      181 ggctcggccg gctcggcggt ggtgcttccg caagaggcgg cgatcttcgt cgacggccgc
      241 tacacgctcc aggtccgcca acaggtcagc gcggaccagt ggagctacca gtcggtgccc
      301 gagaccagca tcaccgactg gctcaaggag catgcgcgtg aaggagcccg gatcggctac
      361 gacccgtggc tgcacagccg cgactgggtg aagagagcaa gcgaggcgct cagccagcgc
      421 ggcgcaagcc tagtcgcggt ccagcgcaac ccgatcgacg cggtgtggga ggatcggccc
      481 gaagcctcca acgcccacct ggtcgtccac cccgaccaat acgccggcaa gagcgccgcc
      541 gaaaagcgca ccgagatcgc cgactggctg agcaaggagg gcgccgacgc ggcggtgctg
      601 tcggcgctcg attcgatcgc ctgggcgttc aacgtccgcg gcgccgacgt cgcccgcacc
      661 ccggtcgcgc tcgcctatgc cttggtccat tccgacggca ccgccgacct gttcgtcgcc
      721 tcggagaagg tcgacgcgca ggtccgccag cacctcggca acggggttcg cctgcacgag
      781 cgcaccgagt tcgagccggc gctcgcggcc ctcgagggca agaccgtcgc cgtcgacccc
      841 gagcgcgcgg tcgcggcgat cttcgaggcg ctcgacaagg ccggggccag gatcctcgcg
      901 gtccgcgacc cgacggtgct tccaaaagcg atcaagaacc cggccgagat cgccggccac
      961 aggtgcgccc aggaacgcga cggggcggcg atcgcgaagt tcctcaaatg ggtcgaggcc
     1021 gaggcgccga agggcgagct cgacgagctc accgcgtccg accgcctcga atccctgcgc
     1081 cgcgaagccc ccgagctccg cgacctgtcg ttcgacacca tctccggcgc cggccccaac
     1141 ggcgccatcg tccactaccg cagttccgaa aagaccaacc gcaagctcga gcccggcacg
     1201 ctctacctgg tcgattcggg cggccaatat gtcgacggca ccaccgacat cacccgcacc
     1261 gtgccgatcg gcgagccgac ggccgagatg cgcgaccgct tcacccgcgt gctcaagggc
     1321 cacatcgccg tcgcctcggc gctgttcccc aagggcacgc gcggcagcca gctcgacagc
     1381 ttcgcccgcc gcccgctgtg ggaggcgggg ctcgactacg cccacggcac cggccacggg
     1441 gtcggcagct tcctgtcggt ccacgagggc ccgcagcgca tcgccccggt cggaagcgcg
     1501 caatcgggcg gcgacgagcc gctgcgcgcc ggaatgatcc tcagcaatga gcccggctat
     1561 tacaagagcg gcgagtacgg catccgcatc gagaatttgg tgctggtcgt cgaccggcag
     1621 gtcgccggcg cggaaaagga gatgctcggc ttcgagaccc tgacccacgc cccaatcgag
     1681 cggcgcctga tcgtccgcga aatgctcggc gaccaggagc tcgcctggct caacgcctat
     1741 cacgccgagg tgctggcgaa gatcggcccc cgcctcgaag gcgccgaccg cgcctggctc
     1801 gaagccgcct gcgccccgct ctaatcctcc ccggaacggg gagggggacc atccggagga
     1861 tggtggatgg ggttcttcgc ctggctcggc gccccctgta gccgtcatcc cggacttgct
     1921 ccgggatctc agcgagtttc agtctccggc gccttcttgt cccgaaatcc cggcctccgc
     1981 cgggatgacg ccttgcgcca tctctcgcgc ctgcgccttg cgccgcttca gattctcctt
     2041 gagcgcggcc gcaagccgtt cctcgcgctc ctttttcttg tcggccatgg tccccatttt
     2101 ggtccccgcg gagaccgacg gcaagccggg ttgacacagc ggcgccctca cggtcaaagg
     2161 cgcgcgtcct ccgtcagtgc tgccgtagct cagtggtaga gcgcatcctt ggtaaggctg
     2221 aggtcgtgag ttcaatcctc accggcagca ccatcctctg accgcgcctg tccgggggac
     2281 aggcgaagtc agaggatccc ccggatgggg tgtggagaaa cccgcagccc gtctgatgca
     2341 gggtcccatt cgctcgcaaa gctaatgtca gccttgggtg gaaagcggac attcgaaggg
     2401 tccgcgataa gcgtcgttgg tcacgactgg tcgatcgcct gctgcaactc ccgggtctgc
     2461 cgctcaactg ccccgaggac gacgaggtat gccgcgaccg ctgcgcgatg ccagcgcagc
     2521 tcgccgacca actcgggccg tgcgcgcagc gcggacgccg cctgcgcgaa gcgggcttcc
     2581 ggcagatcga gatcgcaggt cgccggaaga cgaatggtca gcggccgctc cggaatgggc
     2641 ttatcggagc actcggcccg gatcgcgtcc tggatgtctt cggggatgag ctgccgcaca
     2701 tgttcccggt agggcgtggc aacggccgcc agatccactt gattatagtt gaacgccatc
     2761 aagttggtca ggctgcggcg caccgcgaca ctgtcgatgt cgcgcatccg ctcgccgccg
     2821 aaaaccgacg cccagttggc gccattgact ccgaacctgt agacttggct ggcctggtaa
     2881 gcagcgatca caaattgctc gtcgctgacc ttcggatcgc cgcgccagcc gacgaatgcc
     2941 gtatccgagt aggctcgcga cgtcgcataa taatctttgg cggtttcgaa gaagtccatc
     3001 gaggattgca atgctggccg caattcgagg agtagctggg cagtctcccg cttttcgatc
     3061 cgctgctggt tccagttcga cacctgggta ccaatgaaga cgcccacgat gacgatggcg
     3121 aactcgatcg taatggcggt ccagttctgc gccctaagat tctcaatgat gcggcgaagg
     3181 aacatgtccg gcctcccgct ttcccggcgt tgagaatggc ctgcgttgct agagaaagaa
     3241 aggctgcgac gctaatgacc gcaatgggtc gaaagcggac attagccccc gctgcacttc
     3301 gcaccaatca cccgtagctc ggctcgagca gcccctcgat cacgtggcgc tggataaccg
     3361 gctcgagcgc gtcgctgacg cggcaggcca gatggtcgcg cacccgcggc ggcaggcggt
     3421 cgaggtagcc gcgggtcacc tggacgtcgt ggtggtcggt gttgaccgca tcgggcgcgt
     3481 cgaccccgac gaccagcacc ggccgagcga ggtccgagcg gttggcggtg ccgcggtgga
     3541 tggtcagcgc cgatcgcgcc gacatgtcgc cgcgctgcgg cagcttgcgc accatccgct
     3601 tggcgtagcg gtcccacagc tcgggccccg ggaacatgtc gtccctggcc ccggtgatgt
     3661 cgtcccattg cgtccggagc gcgatctcca gcgcccccat ctcgagcgtg cagtcgacgg
     3721 ccggcaggtt gaacgcgagg ctgttcagcc gcctcccgat gagcgtcgcc tcgggcgccg
     3781 ggaagtcgcg gtgccacggc tggtcggcgg cgccggggaa gggaatgtcg aacccgacct
     3841 cgacgattgt gtagtcgggg ccgagcaccg cctcgcacac cgcgacgaac caggggtggg
     3901 tggcgatgtc ggtaaagccg cggacgcgct cggggtggac ctcgacgtac cagcgctcgg
     3961 gcccgcgcgg caaagcgccg ccgggcaccg ctttggcctc ctcgaacagg gtcatgatgt
     4021 cggcgtgcag cgcgtcggcc cactcgggcg aaaaggcgcc tttcaggccg atgatcccgt
     4081 cgccgtagag cccgcccatg atcgccgcgg cgtcgtactc ctgctgctga agagcgctca
     4141 cccaaaccct ccctcacatc ccgccagcga agatatcacc tttggcttgc catgcgaggc
     4201 tcgatccgct tggacaccgg ccgcgatgcg atttttctcg gacaatgcgg ccgccgcgca
     4261 ccccgcggtg ctcgaggcga tcgccgcctc caaccgcctc gacaccgcct acgacggcga
     4321 cgaatggagc gcccgcctcg acgcggcctt ctccgacctg ttcgagaccc gcgtccgagc
     4381 tttgtgggtg acgacgggaa cggctgcgaa ttgcctcgcg ctcgcggccc tgtgcccgcc
     4441 ccacggcggc atcatctgcc accgggacgc gcacatcgag aacgacgagg ccggcgcccc
     4501 gggcttctac acaaacgggg ccaagctgat cctggtcgac gggccgggcg ccaaggtcac
     4561 gcccgatgcg gtgagcggag cgatcggccg catccgcaag gacgtccacc aggtccagcc
     4621 gcgcgctttg tcgatcacca acgcgaccga atacgggctc gcttttagcg ccggcgaagt
     4681 cgccgcgctc ggcgagctgg cgcgcgcgca ccagctcggc ttccacatgg acggcgcccg
     4741 cttcgccaac gccagcgccg cgaccggcgc ccgccccgcc gatgtcacct ggcgggccgg
     4801 aatcgacgct ctatcgttcg gcttcgtcaa gaacggcggc ctcaacgccg aggcgctggt
     4861 gctgttcaag gccgagctcg ccgacgaaat cctcgtccgc cgcaagcgcg ccggccatct
     4921 gctgtccaag ggccggatgc tcgccgcgca gctcctggcg ctgctcgaca acgacctgtg
     4981 gcttgccaac gcccgcgccg ccaacgccgc cgccaaggct ctcgccgagg ccgcgcccga
     5041 tcgcctcgtc catttggtcg aggccaacga gctgttcctc aaaatgaccg ccgacgaggc
     5101 cgcccggctg cgctcgcagg gcttcgactt ctacgactgg gggacgggcg agatccgcct
     5161 ggtcaccagc tgggaccagc agggcgaggc ggtcggcaag ctggcggcgg cgatccgcgc
     5221 gctgtgacgg ccgcgcttgc ggtcggacgc cgcgccgacc tttcggtcat cgtcccgttc
     5281 ctcatcttca ccgccatctg gggctcgacc tggatcgtca ttcgcgacca gctcggcgcc
     5341 gtcccgccgc aatggtcgat cacctaccgc ttcctcctcg ccacggcggc gatggcggcg
     5401 atcgcccgct ggaagggcgg gggcctgacg ctgcccgcgg gcggaatcgc ggccgcggcg
     5461 gtgctcggct tcacgcaatt cgtgatcaac ttcaacgccg tgtacctggc cgagcagcac
     5521 atcacctcgg gcctggtcgc gacggtgttc gccttgctcc tgattcccaa caccctgctc
     5581 ggctgggttt tcctcggtca gcggccggat gcgcgcttcg tcgccggctc gatggccgcg
     5641 gtcgcgggga tcggcttgct gttcgcccac gagctcggcg agaatccggc gcgcagcggg
     5701 gcgatcctgg ccggaatcgg cctcaccctc gccgggatga tcgcggcgtc gatcgccaac
     5761 gtgttccagg cgcgcgagga ggtgcggcgc taccctttgt tcacgctgct cgcctgggcg
     5821 atgggcctcg gggcgctgat ggacggcgcc ctggcgctcc tgatcgccgg cccgccggtc
     5881 gtcgagctgc gccccggcta ctggctggga atcgtctatc tggcggtgtt cgcgtcggcg
     5941 atcgccttca gcctctatct gccggtggtg cgcaagatcg gcccggccaa ggcggcttat
     6001 tcgagcgtcc tggtgccggt catcgcgatg ggcttctcga ccgcgctcga gggctaccgc
     6061 tggacgccca tggcggccgc cggggcggtg ctcgcgcttg gcggaatgct cggcgcgctc
     6121 agccgctcgc gctcggttgt gccggctccc gacgccgcct aagcccgctc gttcatggac
     6181 gcccgaacga tccccgaaga gccgcgcccg ccggcccatg tcgccatccg gcgcgaagat
     6241 taccgcccgc ccgactggct ggtgcctgag gtcgcgctcg atttcaccct cgaccccgcc
     6301 gcgacgcgcg tccgctcgac gctgaccgtg gtgcgcaacg gcaaccaccg ccgcccgctg
     6361 cggctcgacg gcgacgagct gaccccgctt cgggtcgcgg tcgacggcgg cgaggcgcgc
     6421 tggtcgatgg acgggccggc cctcgtcatc gagctctcaa gcgatagcgc gacggtcgag
     6481 accgaggtcg aggtccaccc ggccgccaac agcaagctga ccggcctcta cgcgtcgggg
     6541 ggcgggctct acacccagtg cgaggcggaa ggcttccgcc gcatcacctt cttccccgac
     6601 cggcctgacg tgctcagccg ctaccgggtg cggatggacg ccgattcggc cgccttcccg
     6661 gtgctgctga ccaacggcaa gtgcgtcgaa tccggccagg gcgaaggcgg ccgccactgg
     6721 gcccggtggg aggacccgtt ccccaagccc tgctatctgt tcgcgctggt cgcaggcgac
     6781 ctcaagccca accgcgacag cttcaccacc atgagcggcc gcaaggtcga tctcgccatc
     6841 tgggtgcgcg aggaagacct tcc
//
LOCUS       DASTZO010000314         9037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1148902,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000314 DASTZO010000000
VERSION     DASTZO010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 9037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9037
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1148902"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VFU20_08835"
     CDS             <1..141
                     /locus_tag="VFU20_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU5286592.1"
                     /translation="PKHLDEKVAALHLGKLGVRLTRLSDKQAAYIGVSPEGPFKPEHY
                     RY"
     gene            295..3585
                     /locus_tag="VFU20_08840"
     CDS             295..3585
                     /locus_tag="VFU20_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5286593.1"
                     /translation="MSSLNARILASASVAVIAIAGLSGTANAQAPAPGPVTPTEAQAC
                     ADLPTQPERDACIAAQARPEGTPEEAGDPATQPAGETAERQAESDAIVVTGSRIRRSA
                     FTSPDPITIIDPELELKGGENDTAEILQTNPVAAGSFQITSLLSAGSFVTNGGVGAQT
                     LSLRGLGAERTLVLVNGRRAGPAGTRGAIAGFDLNVIPSAIIQSVEVVKTGASPIYGS
                     DAIAGVVNLLTRKATEGIDLRGFTSVPLDGGGETYNVSVSYGKDFGRGHLIGGFDYNH
                     RNELERGDRDYLMCPEEFLFNPDGDRVDVPDPRTGEPRCNAFFANAINVSTRTLVQPP
                     GSSPITFGTIQFDGPGDRLGEFLPVMSGIGFTMPTGFFPVALNCNPNASANPTAAQLD
                     LCRRSTGLLDPGAKYVRDVSVFPELDRYTFWADGSFEVTDNIELVGEFLYNKRKTRTD
                     GVRQLFFNQFTGSTGLPAIICASAAQRAATPNCSPTGTGDPLNAGFAGNVFLLPIVPV
                     STFIKTDVDYYRGVGGVRANLGGFMDGWRFDSYVQHSRSDGDYTNHRILDDAVDLQEL
                     RSRTCQPGQVTRVTGTPCMDIDFTDPRVLRGEFTPAERAFLFSSETGNTLYKQTTAEA
                     SLAGDLIDLPAGPAKAAIGVQWRRDQIDDTPGEITLSRNVWGQSVSGRTAGFSTSKEA
                     FGEVDVPLLRDVPFFQSLNVSAAARLTDYRAERDSDELTDRDKNNWTYKLGLNWAVNN
                     WLRFRGTYGTSFRAPALFEQFLADQTGFLGQQAVDPCINFAQRDPSDRIRQRCEALGL
                     PLNFGAAGSSSALVSSGGGIGVLDPETSKAKTVSVVLTPDLGLWSGMRFSVAVDYFDI
                     EVKNQVTTLGGGNIVFGCFESDNYPDDPLCSLFERDLTPGSPRFGQIITIRDPFININ
                     RQHNRGVDVTARINQDLGRHGTLSALGQMTWQIEDLFELFRGFEADDNGESGEPRWVG
                     DFKLTWANGPWSLFYGLNVIGSTSDELDLRNSRAGEVCFNSPLRGGLVCPVYKLDAQF
                     YHSASVTREVGERFTMTLGVNNLFDNNPPRISGSFSPISGLGQVPVFGTQYDLVGRRA
                     FVSVRAKM"
     gene            3625..4428
                     /locus_tag="VFU20_08845"
     CDS             3625..4428
                     /locus_tag="VFU20_08845"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HEU5286594.1"
                     /translation="MRIAYLLPLLIGSVATPAFAQDFGTGGRVELRAGYDEPRFLVEF
                     RDGADTETRSERGLALGIEGGFDVRVLEGLLAGVYAGVETSTVDECDELFGDDRLCID
                     AGLNFTAGGRLGVPTGDGGLIYAKLGYSRGKFRISYDDGVTTLDESESTGGWHVGAGF
                     ELALGGNLYAKAEYIHTRYHTMFKDELTDEDKIEPTRHQLIGGIGIRFGMASAAPPPP
                     PPPPPPPPPPEAPATQTCPDGSVILATDACPPPPPPPPPPPPPASGERG"
     gene            4583..6940
                     /locus_tag="VFU20_08850"
     CDS             4583..6940
                     /locus_tag="VFU20_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HEU5286595.1"
                     /translation="MSELSGAGLVAATAAVLWILIAVALSIAAARRLARAGSVLDSAR
                     SMASLLEVAPARPMLVRTDGRIEADPRLLRELGMDRAPARLADLAGDTCGIEKDDLDA
                     LAADVEAAAIACERVERKVRAAGSKRVFDVRGGPAPPPEPPGTLLLWLFDTSASEEER
                     SKLSLRLRQTEGALDSLTHLIESAPFPMWYRGPDLKLGLVNSAFVDAVEARDAAEVIA
                     KGIELIDEPGDNGARAAAMRALEVGKIQSRNQAAILRGERRMLRIVNVPLPTGAVAGF
                     AIDIQDLEDARFELARHIESQRELADRMTAGAAQFDADRSLSFFNQPFAVMAQLDSDW
                     LAERPEFDRVLERMRDNHRLPETRDFPAWKDERRAWFSSPEEVIEEEWMLANGDHLRV
                     VAQPLPDGGLRLFLEDRTEQVRLASARDTLLRVRAATFDNLFEAISVFASDGRLYLWN
                     RRFCQVWDLDEEWLREHPRVDELVPAMARKLVNPTAAAQVREQVRQTTAKRESAGGRI
                     SMTDGRHFEFAAVPLPDGNALFTMVDVTDSTRIEAALRERATALEEADRVKTDFVANM
                     SYELRTPLTSIGGFAEMLSAGYAGKLTPAAEDYVNAIVESSGRLAKLVDDVLDLTTGD
                     TRGINLERERIDLAGLCRAAVETAQSRAAEKAQKLTSDIDPSTGFVIGDARRLRESIE
                     HVLHNAIAYTDRKGRIALKAKGDDERAVIEISDNGSGIAKEDLPRVFNRFDRVEEAGV
                     RGEAALGLGLPLTRQFIEAHGGTVELESKKGKGTSVTLTVPRGSA"
     gene            6937..7371
                     /gene="tsaE"
                     /locus_tag="VFU20_08855"
     CDS             6937..7371
                     /gene="tsaE"
                     /locus_tag="VFU20_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017666924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU5286596.1"
                     /translation="MILASEAETVALGERIAECARPGDVITLCGPLGVGKTTLVRGIL
                     AGLGHSDEVPSPSFAIVQPYEALDPPAWHVDLYRLEHPSELEELGLDAAGDVVLLVEW
                     PERAGEAAWPHALRLSLDFAEDGARRLTAQVPPAWEGRWPPR"
     gene            7368..8354
                     /locus_tag="VFU20_08860"
     CDS             7368..8354
                     /locus_tag="VFU20_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HEU5286597.1"
                     /translation="MIPPADAPRFLESCGWAGATIEPLAGDASFRRYFRVIGNGRSAV
                     LMDAPPPHEDPRPFAAVAEWLCSAGLSAPEIIARDLDKGLLLLADFGDARLRETLDED
                     PQRERELYELATDVLIHLHRHPPMPGLPVHGLDQWLEELTLFTDWYCPALGLDVDVEG
                     YRAAWVEALGPVESDGLGPVTILRDYHAENIMLIDGRDGVAHVGLLDFQDAHAGHPAY
                     DLASVLEDARRDVPPAIERAMIDRYMAATGHSETFERAYWALAAQRNTRILGVFTRLW
                     KRDHKPHYTQFQPRMWGLLERDLASPQLAPVRAWFDTNVAPEHRAAPWSEAA"
     gene            8417..>9037
                     /locus_tag="VFU20_08865"
     CDS             8417..>9037
                     /locus_tag="VFU20_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5286598.1"
                     /translation="MVMAAGLGKRMRPLTATKPKPLIEVAGKPLLDHVLDRLRAAGVK
                     KVVVNVHYLADSLQAHLERTAAGLEVTISDERELLLETGGGLVKAQAMIGCDPFLAIN
                     SDNLWIDGPADTLKLLASHWDGAKMDALLLLVPQARAMNHRGQGDFHMDRAGRLRRRA
                     RSRVAPFVYTGLQVVSKRLLEGAPEGPFSTNLLWDRAIEKGRCFGAV"
BASE COUNT         1428 a         3179 c         3012 g         1418 t
ORIGIN      
        1 cccaagcacc tcgacgagaa ggtcgccgcg ctccatctcg gcaaattggg ggtccggctc
       61 acgcggctca gcgacaagca ggcggcgtac atcggggtct cgcccgaggg cccgttcaag
      121 cccgagcact accgctactg acgaggcctt tatggccgtc tccaaacaat taatgttgta
      181 ttaatgtgac aggtagcggt tttccgtaac cccctgaacc ggtgccattt gcatttgccg
      241 tatcggcgct aatctagtcc aatctacacc tgtatctcct ggggaattcg atacatgagc
      301 agcttgaacg cgcggatcct cgcatccgca tccgttgcgg tcatcgccat cgccggcctg
      361 tccggaaccg ccaacgcgca ggctccggca cccggcccgg tcactccgac cgaggcgcag
      421 gcgtgcgccg acctgccgac gcagccggag cgcgacgcat gcattgccgc gcaggctcgt
      481 cccgaaggca ctccggaaga ggccggtgac ccggcgactc agcctgccgg cgagaccgcc
      541 gagcgccagg cggagtccga cgccatcgtc gtcaccggct cgcgcattcg ccgcagcgca
      601 ttcaccagcc cggacccgat caccatcatc gaccccgagc tcgagctgaa gggcggcgag
      661 aacgacaccg ccgagatcct gcagaccaat ccggtcgctg cgggctcgtt ccagatcacc
      721 tcgctgctgt cggcgggctc gttcgtgacc aatggcggcg tcggcgcgca gaccctgtcg
      781 ctgcgcggtc tcggtgccga gcgcacgctg gtcctggtca acggacgccg cgccgggccg
      841 gccggcacgc gcggcgcgat cgccggcttc gacctcaacg tcatcccctc ggcgatcatc
      901 cagtcggtcg aagtcgtgaa gaccggcgcg tctccaatct acggctcgga cgcgatcgcc
      961 ggagtggtca acctgctcac ccgcaaggcg accgagggga tcgacctgcg cggcttcacc
     1021 tcggttccgc tggacggtgg cggcgagacg tacaatgtca gcgtgtccta cggtaaggac
     1081 ttcggccgcg gccacctgat cggcggcttc gactacaatc accgcaatga gcttgagcgc
     1141 ggcgatcgcg actatctgat gtgtccggaa gagtttctgt tcaacccgga cggcgatcgc
     1201 gtcgacgtcc ccgatccgcg gaccggcgag ccgcgctgca acgccttctt cgccaacgcc
     1261 atcaacgtca gcacccgcac tctggtgcag ccgccgggat cgtctccaat caccttcggc
     1321 acgatccagt tcgatgggcc gggcgaccgg ctgggcgagt tcctgccggt gatgtcgggc
     1381 attggcttca ccatgcccac gggcttcttc ccggtcgccc tgaactgcaa cccgaacgcg
     1441 tccgcgaacc cgacggccgc ccagctcgat ctttgccgcc ggtcgaccgg cctgctcgac
     1501 ccgggcgcca agtacgtcag ggacgtgtcg gtcttcccgg aactcgaccg ctacactttc
     1561 tgggcggacg gctcgttcga agtcaccgac aatatcgagt tggtcggcga gttcctgtac
     1621 aacaagcgca agacccgcac cgacggcgtc cgccagctgt tcttcaacca gttcaccggc
     1681 tcaacgggcc tgcccgccat aatctgcgcc agcgccgcgc agcgggcggc caccccgaac
     1741 tgctccccga ccgggaccgg cgatccgctc aatgccggct tcgctggaaa cgtcttcctg
     1801 ttgccgatcg tcccggtttc gaccttcatc aagaccgatg tcgattatta tcgcggggtc
     1861 ggcggcgtca gggccaacct cggcggattc atggacggat ggcgtttcga cagctatgtc
     1921 cagcatagcc gttcggacgg tgattacacc aaccaccgaa tcctggacga cgcggtcgac
     1981 cttcaggagc tgcgctcgcg cacctgccag cccggccagg tgacccgcgt caccggtacc
     2041 ccatgcatgg acatcgactt caccgatcct cgggtccttc gcggcgaatt cacgccggcg
     2101 gaacgggcat tcctgttcag ctcggaaacc ggcaacaccc tctacaagca gacaaccgcc
     2161 gaagcgagcc tcgccggcga tctcatcgac cttccggccg gtcccgccaa agcggctatc
     2221 ggcgttcagt ggcgccgtga ccagatcgac gacactccgg gcgagatcac tttgtcgcgc
     2281 aacgtctggg gccagtcggt ctcgggccgc accgccggct tctcgaccag caaggaggcg
     2341 ttcggcgagg tcgacgttcc gctgcttcgg gacgtcccgt tcttccagag cctcaacgtc
     2401 tctgccgcgg ctcgcctcac cgactatcgc gccgagcgcg attcggacga gctgaccgat
     2461 cgggacaaga acaattggac ctacaagctc ggcctcaact gggcggtcaa caactggctg
     2521 cgtttccgcg gcacttacgg cacgtcgttc cgagccccgg ccctgttcga acagttcctt
     2581 gccgaccaga cgggcttcct cggccagcag gccgtcgacc cgtgcatcaa tttcgctcaa
     2641 cgggacccca gcgaccgaat ccggcagcgc tgcgaagccc tcggcctgcc gttgaacttc
     2701 ggcgcggcgg gcagcagctc ggcgctggtc tcgagcggtg gcggcatcgg cgttctcgat
     2761 ccggaaacgt ccaaagccaa gacggtgagc gtcgtgctga ccccggacct tggcctgtgg
     2821 agcggcatgc ggttcagcgt cgcggtcgac tatttcgaca tcgaggtgaa gaatcaggtc
     2881 accacccttg gcggcggcaa catcgtgttc ggatgcttcg aatccgacaa ttatcccgac
     2941 gacccgctct gcagcctgtt cgagcgcgac ctgactccgg gctcgcctcg cttcggccag
     3001 atcatcacca tccgcgaccc gttcatcaac atcaaccgcc agcataaccg cggcgtcgac
     3061 gtgaccgcgc gaatcaacca ggacttggga cgccacggga ccttgtcggc gcttgggcag
     3121 atgacctggc agatcgaaga cctcttcgag ctgttccgcg gcttcgaggc cgacgataac
     3181 ggcgagtccg gcgagccgcg ctgggtgggc gacttcaagc tcacctgggc gaacgggccg
     3241 tggtcgctgt tctacgggct caatgtgatc ggctcgacct cggacgagct ggacctgcgc
     3301 aacagccggg ccggcgaagt ctgcttcaac agcccgcttc gcggcggcct ggtctgcccg
     3361 gtctacaagc tcgacgcgca attctaccac agcgcgtcgg tcactcgcga agtgggtgag
     3421 cgcttcacca tgacgctcgg cgtgaacaac ctgttcgaca acaacccgcc gcgcatttca
     3481 gggtcgttct cgccgatttc cggcctcggc caggtgccgg tcttcggcac ccagtacgac
     3541 ctggtcgggc gccgcgcctt cgtcagcgtc cgggccaaga tgtaggcgtc gacggtcagc
     3601 aaaagtgatc tggaaaggtt agccatgaga attgcttatc tactgccgct actgatcggc
     3661 tccgtcgcca ctcccgcctt tgcgcaggac ttcggcacgg gtgggcgcgt cgagcttcgc
     3721 gcgggttatg acgagccgcg cttcctcgtc gagttccggg acggggccga caccgaaacg
     3781 cggtcggaaa gaggcctcgc gcttggaatc gaaggcggtt tcgacgtccg ggttctcgag
     3841 ggcctgctgg cgggggtcta cgcgggggtc gaaacttcga ccgtcgacga gtgcgacgaa
     3901 ctgttcggcg acgatcggtt gtgcatcgat gccggcctca acttcacggc cggcggccgt
     3961 ctcggggtgc cgaccggcga cggcgggctg atctacgcca agctcggtta ttcgcgcggc
     4021 aaattccgga tcagctacga cgacggcgtc acgaccctcg acgaaagcga atcgaccggc
     4081 ggttggcatg tcggcgccgg cttcgagctc gccctcggcg gcaacctcta cgccaaggcc
     4141 gaatatatcc acacccgcta ccacaccatg ttcaaggacg agctgaccga cgaggacaag
     4201 atcgagccga ctcgtcacca gctcatcggc gggatcggca tccgcttcgg gatggcgtcg
     4261 gccgcgccgc cacctccgcc gcctccgccc ccgccgccgc cgcctcccga ggctccggcg
     4321 acgcagacct gcccggacgg atcggtgatc ctggcgacgg acgcatgccc gccgccgccg
     4381 cctccgccgc cgccacctcc gccgcctgcg agcggcgaac ggggctgaag agcggagcct
     4441 ccaggccaaa ccttccaggg ccgctccttc gggggcggcc ttttcttttg ccggtccgcg
     4501 ggcggctcgc gaccggtcgt tttcgcttta acggcgacga ccttgcatcc tatgacccgg
     4561 tcatgacggg cgggcgggga cgatgagcga gctgtcgggc gcgggcctgg tcgcggccac
     4621 cgccgcggtc ctgtggatcc tgatcgcggt ggcgctctcc atcgccgcgg cgcggcggct
     4681 cgcccgggcc gggtcggtgc tcgattccgc gcgctcgatg gcgagcctgc tcgaggttgc
     4741 cccggcgcgg cccatgctgg tccggaccga cggccggatc gaggccgatc cgcggctgct
     4801 tcgcgagctc gggatggacc gcgcgcccgc ccggctcgcc gacctggccg gcgacacttg
     4861 cggcatcgag aaggacgacc tcgacgccct ggccgccgac gtcgaggccg ccgcgatcgc
     4921 gtgcgagcgg gtcgagcgca aggtccgtgc cgccggatcg aaacgggtgt tcgatgtccg
     4981 cggcgggccg gcgccgccgc ccgagccgcc cggaacgctg ctgctgtggc tgttcgacac
     5041 cagcgccagc gaggaagagc gctcgaagct gtcgcttcgg cttcgccaga ccgagggggc
     5101 gctggattcg ctcacgcacc tgatcgaatc cgcgccattc ccgatgtggt accgggggcc
     5161 cgacctcaag ctcggcctgg tcaacagcgc gttcgtcgac gcggtcgagg cccgggacgc
     5221 ggccgaagtg atcgccaagg gcatcgagct gatcgacgag ccgggcgaca acggcgcccg
     5281 cgccgcggcg atgagagcgc tcgaggtcgg caagatccag tcgcgcaacc aggcggcgat
     5341 cctgcgcggc gagcggcgca tgctgcggat cgtcaacgtg ccccttccga ccggcgcggt
     5401 ggccggcttc gcgatcgaca tccaggatct cgaggacgcg cgcttcgaac ttgcccggca
     5461 catcgaatcg cagcgcgagc tggccgaccg gatgaccgcc ggcgcggctc agttcgatgc
     5521 cgaccgctcg ctgagcttct tcaaccagcc gttcgcggtg atggcccagc tcgactccga
     5581 ctggcttgcc gagcgccccg agttcgaccg cgtgctcgag cggatgcgcg acaatcaccg
     5641 gctgcccgaa acccgcgact tccccgcctg gaaggatgag cgccgagcct ggttcagcag
     5701 cccggaggaa gtgatcgagg aggaatggat gctggccaac ggcgaccatc tgcgggtcgt
     5761 cgcccagccg cttcccgacg gcggcctgcg cctgttcctc gaggaccgca ccgagcaggt
     5821 gcgcctggca tcggcccgcg acaccctgct gcgggtccgg gcggcgacgt tcgacaatct
     5881 gttcgaggcg atcagcgtgt tcgcaagcga cggccggctg tacctgtgga accgccgctt
     5941 ctgccaagtc tgggacctcg acgaggagtg gcttcgagaa catccgcggg tcgacgagct
     6001 ggtgccggca atggcgcgca agctggtcaa tccgacggcg gccgcgcagg tccgcgagca
     6061 ggtccgccaa acgaccgcca agcgcgagtc ggcgggcggc cggatctcga tgaccgacgg
     6121 ccgccatttc gagttcgcgg cggtgccgct gcccgacggc aatgcgctgt tcaccatggt
     6181 cgacgtgacc gactcgacgc ggatcgaggc cgctttgcgc gagcgcgcca ccgcgctcga
     6241 ggaagccgac cgggtcaaga ccgacttcgt cgccaacatg agctacgagc tgcgcactcc
     6301 gctgacctcg atcggcggct tcgcggagat gctgtcggcc ggctatgcgg gcaagctgac
     6361 cccggcggcc gaggattatg tcaacgcgat cgtcgaatcg tcggggcgtc tcgccaagct
     6421 ggtcgacgac gtgctcgacc tgacgaccgg cgacacgcgc gggatcaacc tcgagcgcga
     6481 gcgcatcgac ctcgccggcc tgtgccgggc ggcggtcgag accgcccagt cgcgcgcggc
     6541 ggagaaggcg cagaagctga ccagcgacat cgacccgtcg accggcttcg tcatcggcga
     6601 tgcccgcagg ctgcgcgaat cgatcgagca cgtgctccac aacgcgatcg cctacaccga
     6661 ccgcaagggc cgcatcgcgc tcaaggccaa gggggacgac gagcgcgcgg tgatcgagat
     6721 cagcgacaac ggctcgggca tcgccaagga ggatttgccg cgcgtgttca accgcttcga
     6781 ccgggtcgag gaggcgggcg ttcgcggcga ggcggcgctc ggcctcggac tgccgctgac
     6841 tcgccagttc atcgaggcgc acggcggcac ggtcgagctg gagtcgaaga agggcaaggg
     6901 cacctcggtc accctgactg tgccgcgcgg ctcggcgtga tcctggcgag cgaagcggag
     6961 accgttgcgc tcggcgagcg gatcgccgag tgcgcccggc ccggcgacgt gatcacgctg
     7021 tgcgggccgc tcggcgtcgg caagacgacg ctggttcgcg gcatcctcgc cggactcggc
     7081 cattcggacg aggtgccgag cccgagcttt gcgatcgtcc agccctacga ggcgctcgat
     7141 ccgccggcct ggcacgtcga tctctaccgc ctcgagcatc cctcggagct cgaggagctg
     7201 ggactcgacg ccgccggcga cgtcgtgctg ctggtcgagt ggcccgagcg cgccggcgaa
     7261 gcggcgtggc cgcacgcgct ccgactgtcg ctcgacttcg ccgaagacgg cgcacggcgc
     7321 ttgacagccc aagtgccccc ggcatgggaa gggcgatggc cgccccgatg attccgcccg
     7381 ccgatgcgcc gcgcttcctg gaatcctgcg ggtgggccgg cgcgaccatc gagccgctcg
     7441 ccggggacgc ctcgttccgg cgctatttcc gggtgatcgg caacggccgc tcggcggttc
     7501 tgatggacgc gccgccgccg cacgaggacc cacggccgtt cgccgcggtg gccgaatggc
     7561 tgtgctcggc cgggctgagc gccccggaga tcatcgcccg cgacctcgac aaggggctgc
     7621 ttctgctcgc cgacttcggc gacgcccggc tgcgcgagac gctcgacgag gacccgcagc
     7681 gcgagcgcga gctctacgag ctggcgaccg acgtcctgat ccacctccac cggcacccgc
     7741 cgatgcccgg gctgccggtg cacgggctcg atcagtggct cgaggaattg acgctgttca
     7801 ccgactggta ttgcccggcg ctcggcctcg acgtcgacgt cgagggctat cgagccgctt
     7861 gggtcgaggc gctggggccg gtcgagtccg acggcctggg cccggtgacg atccttcgcg
     7921 actatcacgc cgaaaacatc atgctgatcg acgggcgcga cggcgtcgcc catgtcgggc
     7981 tgctcgactt ccaggacgcg cacgccgggc atcccgccta cgacctcgcc tcggtgctcg
     8041 aggatgcgcg ccgcgacgtg ccccccgcga tcgagcgcgc catgatcgac cggtacatgg
     8101 ccgcgacggg gcacagcgag acgttcgagc gcgcttattg ggcgctcgcg gcgcagcgca
     8161 acacccgcat cctgggagtc ttcacgcgcc tgtggaagcg cgaccacaag ccgcattaca
     8221 cccagttcca gccgcgcatg tgggggcttc tcgagcggga cctcgccagc ccgcagctgg
     8281 cgccggtgcg cgcctggttc gacaccaatg tcgcgccgga gcaccgcgcc gcgccctggt
     8341 ccgaggcggc gtgagcgggg ctcgccgagc gttgcggctg cgcgccgagg tcgcggccga
     8401 ggtcccggac acggcgatgg tgatggccgc cggcctcggc aagcgcatgc ggccgctgac
     8461 cgcgaccaag cccaagccgc tgatcgaggt cgccggaaag ccgctgctcg accatgtcct
     8521 cgaccgactg cgagccgcgg gcgtcaagaa ggtcgtggtc aacgtccatt acctggccga
     8581 ctcgctccag gcgcatctcg agcgcaccgc ggccgggctt gaggtgacca tttccgacga
     8641 gcgcgagctg ctgctggaga ccgggggcgg gctggtgaag gcgcaggcga tgatcggctg
     8701 cgacccgttc ctggcgatca acagcgacaa tctgtggatc gacggccccg ccgacaccct
     8761 gaagctgctg gcgtcgcact gggacggggc gaagatggac gcgctgctgc tgctggtgcc
     8821 gcaggcgcgc gccatgaacc accgcggcca gggcgacttc cacatggacc gggccggccg
     8881 gctgcgccgc cgcgcccgct cgcgggtcgc gccgttcgtc tacaccgggc tgcaggtcgt
     8941 ttcgaagcgg ctgctcgagg gcgctcccga agggccgttc tcgacaaacc tgctgtggga
     9001 ccgggcgatc gaaaaggggc gctgtttcgg ggccgtc
//
LOCUS       DASTZO010000315         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1018334,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000315 DASTZO010000000
VERSION     DASTZO010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1018334"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..374
                     /locus_tag="VFU20_08870"
     CDS             105..374
                     /locus_tag="VFU20_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286599.1"
                     /translation="MNVLTALAVPAMLFAAGHAAAQPAPSPHEQHQQAGQHPATQTEK
                     GCCCDEKMRQMMMEMMRKHRQGGTPQPKSKTDRQAPAGGDHQHDG"
     gene            394..1803
                     /locus_tag="VFU20_08875"
     CDS             394..1803
                     /locus_tag="VFU20_08875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5286600.1"
                     /translation="MRLTSLGGAGTVTGSKHLVEHDGRRLLVDCGLFQGPRELRELNW
                     KPLSVPASSIDAVVLTHAHLDHSGYLPRLVRDGFRGPIYATSATVAVARLILLDSAHL
                     QEKDADYANRRGFSRHKPALPLYTAQDAEQALAQFRAIDFHKQVAIPGGARLLYRRAG
                     HILGAATAELRLGDQTIVFSGDLGRFGDPTMVDPEPVPDSDFLVLESTYGDRIHPKVD
                     AAAALHEVIDRTSRRGGTVIIPAFAVGRTQSLLYELWTLKMTGRLGLVPVYVDSPMAT
                     NATDLMCAHMDDHRMQPMVCRDACAIATYVRDTESSKALSANPMPKVIISASGMATGG
                     RVLHHIKAFGTDPRNTILFAGFQAIGTRGRAMVDGVREVRIHGQWIDIRAEVADLTML
                     SAHADSNELMRWLRGFRSPPARTFIVHGEPASSAALRDRIESELGWSCVVPRMDEGFA
                     LEPVAGPHEILEEPASRSA"
     gene            1814..>2659
                     /locus_tag="VFU20_08880"
     CDS             1814..>2659
                     /locus_tag="VFU20_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004207026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEU5286601.1"
                     /translation="MRKRTSKSRTAEASGAAGKAHADHRHHPHTHCSDRDDRHLNRPD
                     EDPAAAAPSADAWPEAAQGTIWTCPMHPQIRRNGPGQCPICGMALEPLEPTLEDGPNP
                     ELTDMNRRFWASAALSLPLVLLVVGGELLGWQPLPMRTSMWVQLALATPVVLWGGWPF
                     FDRFWASLRTRNLNMFTLIGLGVGVAYGYSVVGTLAPQIFPASLRTMGGLVPVYFEAA
                     AVITTLVLLGQLLELRARSATGKAIRALLGLAPKTARRVSAAGDEEDIALADVHVGDL
                     LRVRPG"
BASE COUNT          432 a          922 c          885 g          420 t
ORIGIN      
        1 gggcaccctc tggtcgctcc tgttcggtgc gctcgtcggc gcggtgttcg ccttgatcta
       61 caacgcaacc gcggccctcg cccgcccgta aggagcatcg gatcatgaat gtcctgactg
      121 cactcgcggt cccggcgatg ctgttcgcgg ccggtcatgc ggcggcccag cccgctccgt
      181 cgccgcacga gcagcatcag caagccggcc agcacccggc gacgcaaacc gaaaagggct
      241 gctgctgcga cgagaagatg cgccagatga tgatggagat gatgcgcaag catcggcaag
      301 gcggcacgcc gcagccgaag tcgaagacgg atcggcaggc tcccgccggc ggcgaccatc
      361 agcacgacgg ctaggaccgg cggcaaggtg gcgatcaggc tgaccagctt gggaggagcc
      421 ggcaccgtca ccggctccaa gcatctggtc gagcatgacg ggcggcggct gctcgtcgat
      481 tgcgggctgt tccagggccc gcgcgagctt cgcgagctca actggaagcc gctttcggtg
      541 cctgcctcgt cgatcgacgc ggtggtcctg acccacgcgc acctggacca tagcggatac
      601 ctgccgcggc tcgtccgcga cggcttcagg ggaccgatct acgcgacgtc cgccacggtc
      661 gcggtcgcaa ggctgatcct gctcgacagc gcccatctgc aggaaaagga cgcggactat
      721 gccaaccgcc gcggcttcag ccggcacaag cccgcgctgc cgctctacac ggcgcaagac
      781 gccgagcagg cgctggcaca attccgggcg atcgatttcc acaagcaggt ggccatcccc
      841 gggggagcac gcctgctcta caggcgcgcc gggcacatct tgggggcggc cacggcggag
      901 ctccggcttg gcgatcagac gattgtcttc tccggtgact tggggcgctt cggcgatccg
      961 accatggtcg atcccgagcc ggttccggac tcggactttc tggtgctgga gtcgacctat
     1021 ggcgatcgca tccaccccaa ggtcgacgcg gcggcggcgc ttcacgaggt gatcgacagg
     1081 acctcccggc gcggcggaac cgtcatcatt cccgccttcg cggtcggccg gacccaatcc
     1141 ctgctctacg aactgtggac gttgaagatg acgggccggc tgggcctggt gcccgtctat
     1201 gtcgacagcc cgatggcgac caacgccacg gacctgatgt gcgcgcacat ggacgatcat
     1261 cgaatgcagc cgatggtctg ccgcgatgcc tgcgcgatcg cgacctacgt gcgcgacacg
     1321 gaaagctcca aggccttgtc cgcgaacccg atgcccaagg tgatcatctc ggcgagcgga
     1381 atggcgaccg gcgggcgagt gctgcatcac atcaaggcgt tcgggaccga cccgcgcaac
     1441 accatcctgt tcgccggctt ccaggccatc ggcacccgcg gccgggccat ggtcgacggc
     1501 gttcgcgagg tcaggatcca cggccagtgg atcgacattc gagccgaggt tgccgacctg
     1561 acgatgctgt ccgcccacgc cgattcgaac gagctcatgc gctggttgcg cgggttccgg
     1621 tcgccgccgg ccaggacctt catcgtgcac ggcgagccgg cctcgtcggc ggcgcttcgc
     1681 gaccggatcg agtccgaact gggctggtcg tgcgttgtcc cgcgaatgga cgagggtttt
     1741 gcgctcgaac cggtggccgg cccgcatgaa attctggagg aacccgcctc cagatccgcg
     1801 taagcactgc agcgtgcgca agcgaaccag caagagtcga accgccgagg cgtcgggcgc
     1861 cgctggaaaa gcgcatgccg atcatcgcca ccatccccac acgcattgca gcgacaggga
     1921 cgatcgccat ctgaaccggc cggacgagga cccggccgcg gccgcgcctt cggcggacgc
     1981 ttggcccgag gcggcgcagg gcacgatctg gacttgcccg atgcacccgc agatccgccg
     2041 caacgggccc ggccaatgtc ccatctgcgg catggcgctg gagccgctcg agccgacgct
     2101 cgaggacggc cccaatcccg agctcaccga catgaaccgg cgcttctggg caagcgcggc
     2161 gctgtccctg ccgctcgtcc tgctggtcgt cggcggcgag ttgctcggct ggcaaccgct
     2221 gccgatgcga acctcgatgt gggtgcagct cgcgcttgca acgcccgtcg tcctgtgggg
     2281 cggctggcca ttcttcgacc gcttctgggc atcgctgagg acccgcaacc tcaacatgtt
     2341 caccctgatc ggtctcgggg tcggagtcgc ttatggctac agcgtcgtcg gaaccctagc
     2401 gccgcagatc ttcccggcct cgcttcggac catgggcggg ctcgtgcccg tctatttcga
     2461 agccgcggcc gtcatcacga ccctggtcct gctcggccag ctgctcgagc tcagggcaag
     2521 gtcggcgacc ggcaaggcga ttcgcgcgct gctcggcctt gctccgaaaa ccgctcgccg
     2581 ggtcagtgcg gccggcgacg aggaggacat tgcgctcgcc gacgtccatg tcggcgacct
     2641 gcttcgagtc cggccggga
//
LOCUS       DASTZO010000316        12441 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_385500,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000316 DASTZO010000000
VERSION     DASTZO010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 12441)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12441)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12441
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385500"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..728)
                     /locus_tag="VFU20_08885"
     CDS             complement(45..728)
                     /locus_tag="VFU20_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286602.1"
                     /translation="MKALRLFAQLSAYYLVIAVLVYGALTLFPWVRDYLPVGGVEELI
                     NQPQQAGANGLTGVQVQQTENVANLGESLFWLTTAIVGALLTALPVSWTYMAIRHGDE
                     YDQSLISTIIILPLVVTGIVVIVQNSLALAFSLAGIAGAVRFKNSLKSSGDALFILLS
                     VGIGLSAGIGAVELAVVMSVAFNYGFLALWLTEYGEREGMKRYMSDYEVDEGAAPPSA
                     PAPGLPPTE"
     gene            complement(801..1577)
                     /gene="ppk2"
                     /locus_tag="VFU20_08890"
     CDS             complement(801..1577)
                     /gene="ppk2"
                     /locus_tag="VFU20_08890"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HEU5286603.1"
                     /translation="MAELSQDEYERLLKPLTKELSGMARWVAETGQRIVVLFEGRDTA
                     GKGGSIDMIARVLNPRQCHVVALASPNERERAQWYFQRYTPHLPPRGEIALFDRSWYN
                     RAGVEPVMGYCSQAETDAFLRAVPEFERHLVDDGILLFKYWLCCDQDKQEHRFENRLR
                     NPLKRWKLSPIDIEARSKYDAYTEAREKMLAATHTDFAPWTLVDFNDQALGRLTLLRD
                     LLNRIPDTELPLNEIPWPPLPGPPGKERFGVLRPIPNYQA"
     gene            1791..2606
                     /locus_tag="VFU20_08895"
     CDS             1791..2606
                     /locus_tag="VFU20_08895"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU5286604.1"
                     /translation="MFLRTLSRGALAALLLSAVPVVAPTAAVAASPAAGLAVPADFGS
                     PVYPKRGRYLLVDAASAKLFMVEDGQVVDTMRVIVGKPDAQTPTIASKIYYATLNPYW
                     NVPADLARKIIAPRVLEKGVGYLKQNGYQVLASFDDGAPEISPREVDWKAVAAGAETV
                     KIRQLPGPGNSMGQVKFGFPNGFGIFLHDTPKKELFAMPERAISNGCVRLEDAPKLAR
                     WLLGRDPSAIVASSEPEQHVPLPRAVPIYITYLDRQPAELALAPSTTSSPLTR"
     gene            complement(2610..3026)
                     /gene="rplQ"
                     /locus_tag="VFU20_08900"
     CDS             complement(2610..3026)
                     /gene="rplQ"
                     /locus_tag="VFU20_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU5286605.1"
                     /translation="MRHRVGHRKLQRTSSHRTALFRNMAAALIKHEQITTTTAKAKEL
                     RPYVEKLVTLAKKGGLSNRRLAQGRLMDEAQLAKLFDVIGPRYAGRAGGYTRIIKAGI
                     RASDAAPIAIIEFVDRDPAAKGQDSGPVMTDEEIAA"
     gene            complement(3117..4181)
                     /locus_tag="VFU20_08905"
     CDS             complement(3117..4181)
                     /locus_tag="VFU20_08905"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013039135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU5286606.1"
                     /translation="MAVNAKNWQELKKPNALERKIGGDARRRASFVAEPLERGFGMTL
                     GNSLRRVLLSSLQGAAITSIKIEGVLHEFSSLTGVREDVTDIVLNVKQIALRMEGEGP
                     KRLQLSATGPTDVTAGMIATTGDIEVTNPDQLICHLDEGATLNMELTADVGKGYVPSQ
                     ANRPADAPIGLIPVDALYSPVRQVAYKVENTRVGQELDYDKLTLTIETDGTISPEDAL
                     GYAARILQDQLQLFVHFEEGAIRAPQPAMVGGMPTPVEAQADTNQLNRYLLKKVDELE
                     LSVRSANCLKNDNIIYIGDLVQKTEAEMLRTPNFGRKSLNEIKEVLASMGLRLGMDIP
                     GWPPENIEEMAKKLEQEMLG"
     gene            complement(4269..4658)
                     /gene="rpsK"
                     /locus_tag="VFU20_08910"
     CDS             complement(4269..4658)
                     /gene="rpsK"
                     /locus_tag="VFU20_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU5286607.1"
                     /translation="MAQAPQRLRRRERKNITAGVAHVNASFNNTMITITDAQGNAIAW
                     SSAGMMGFKGSRKSTPYAAQVAAEDAGKKAADHGVRTLEVEVKGPGSGRESALRALQA
                     VGFQITSIRDVTPIPHNGVRPSKRRRV"
     gene            complement(4786..5154)
                     /gene="rpsM"
                     /locus_tag="VFU20_08915"
     CDS             complement(4786..5154)
                     /gene="rpsM"
                     /locus_tag="VFU20_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011540878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU5286608.1"
                     /translation="MARIAGVNIPTNKRVEIALTYIHGIGRSKARQITEKLGIGPEKR
                     VQDLSDQEVLHIRETIDSDHNVEGDLRRETAMNIKRLMDLACYRGLRHRKGLPVRGQR
                     THTNARTRKGKAKPIAGKKK"
     gene            5371..6300
                     /locus_tag="VFU20_08920"
     CDS             5371..6300
                     /locus_tag="VFU20_08920"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU5286609.1"
                     /translation="MERLTPDQPLPERLRAGIVALGNFDGFHLGHQAVVGRAVARGHH
                     ERRPVIVATFDPHPVRLFKPEVPPFRLTSLDQRERLFAHAGADAMLVFRFDQQLAATS
                     AEDFVAELLGRRIGAAGVVTGDDFTFGKGRAGNSETLRSLGLRVGIAAEAVPPVLLDG
                     TRISSGRIREALAAGDPGTATRLLSRPFAVEAVVERGDGRGRDLGYPTANLRLGDYQR
                     PAYGIYAMRVRLDDEREFPGVGSLGVRPTFEPPIELLEAHLFDFDEDLYGRTIEVALH
                     AFIRPEEKFADAAALAARMKEDEAEARKLLALA"
     gene            6340..9222
                     /gene="ileS"
                     /locus_tag="VFU20_08925"
     CDS             6340..9222
                     /gene="ileS"
                     /locus_tag="VFU20_08925"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU5286610.1"
                     /translation="MSDAPEKPNYRETVFLPRTDFPMKAGLPQKEPAILARWADQDLY
                     GQVRKARAGRDKFILHDGPPYANGDIHIGHALNHVLKDMVVRTQTLLGKDAPYVPGWD
                     CHGLPIEWKIEEQYRKKKLDKDEVPLTEFRAECRAYAQHWVETQREQLKRLGIGGDWD
                     NPYLTMDYEAEATIVAELMKFAESGQLYRGAKPVMWSPVEKTALAEAEIEYEDVVSTQ
                     IDVAFEIVESPIPELVGAYAVAWTTTPWTIPANQAIGYGPEIEYGLYEIWLNVSDAGD
                     GDLSLEQRRNPLMDTLDKRRILIAKNLKEAFKERLNRGLKDKSTQAAVQLSAALKDLQ
                     SLHGLGLAGSIARHPMHHLGGFFARPRPFLAAPFVTTDQGTGLVHEAPDHGEEDFYLC
                     QQHGIDPVFAVTDDGRYRDDWAWLGGQGSVINAKFNAPDGPICSDLRETGALLAASAD
                     FTHSYPHSWRSKAKVIYRCTPQWFIAMDRPLDSGRTLRETAVQAIADSRFVPGKGRNR
                     LGSMVAERPDWVISRQRAWGVPIALFVERKTGELLVDPEVNKRIVEAVRAGGVDAWND
                     ANAAQLLGAGRDPADYERQTDILDVWFDSGCTHVFTLESGRWPDQRWPADLYLEGSDQ
                     HRGWFQSSLLESCGTRGRAPYDAVLTHGFTMDSKGMKMSKSLGNVINPLDLMKEHGAD
                     ILRLWALSVDFTEDHRIGKEILGGVADQYRKLRNTFRYLLGALERFSDDERVADVTAM
                     PELERYMLHLTAELDAKLRAAVGEFDFNTYVRAITDFCNEDLSAFYFDIRKDVLYCEV
                     NPATGRQTDKRRSYRTVLDTLFHALVRWLAPVLVFTTEEVWGTRFPDGGSVHLLEWPE
                     IPAVAADDEKWAKLRELRTAVTEAIEPLRRDKVLGSSLEAEVTLPEADASLDLAELFI
                     VSAVQPGDGLKVAKTANRKCGRCWRHLPEVSEDGGLCARCEEVLNG"
     gene            9215..9715
                     /gene="lspA"
                     /locus_tag="VFU20_08930"
     CDS             9215..9715
                     /gene="lspA"
                     /locus_tag="VFU20_08930"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU5286611.1"
                     /translation="MAERRFGFGIALVVFALDQLTKWLMIGPLELQSVRQIVLLPIFN
                     LSWTENYGISLGLLNAETEVGRWMLVAVTAAIAVGVAIWIGREKQRGDRLALGMVLGG
                     ALGNILDRVRFGYVVDFADLHFGTFRPFLVFNVADAAISIGVVILLLRAFLIRDERPK
                     ENIEHA"
     gene            9708..10118
                     /locus_tag="VFU20_08935"
     CDS             9708..10118
                     /locus_tag="VFU20_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007165633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3035 domain-containing protein"
                     /protein_id="HEU5286612.1"
                     /translation="MRNWNSALLLVPALALGGCAMLGGGNRAPDEFAVVRNAPLIIPP
                     DYTLTPPVAGTAAVSPAEAQAQAIEALFGGPAPRSNAETSLLERAGRDRAAVGARSSV
                     GDPNTRVVDKGTVTAQILVAPQADGRDATVQVPR"
     gene            10148..10699
                     /locus_tag="VFU20_08940"
     CDS             10148..10699
                     /locus_tag="VFU20_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU5286613.1"
                     /translation="MAEESLSARLAEVESMIDRSRRRFEEGSRLVEEAHKALADLQQE
                     LIGSPVTLSPEDSEEASSRLLTSIKAAGGEMPETLCGGRLSVDQVQRLIRIDGHPIGI
                     TEMEYRVLELLAFARNNVVTRAMLLKHLYRRADDQPQPKIIDVFISKLRKKLRMASSG
                     HEFIETIPQRGWILRDIEAGSGA"
     gene            10752..11111
                     /locus_tag="VFU20_08945"
     CDS             10752..11111
                     /locus_tag="VFU20_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286614.1"
                     /translation="MLNFVSVPQADDPPEPYAEALARLASIRHALRLVDPFGGGPASD
                     PSEDDAIAAAWTGAGKAAARCFDGRTARTAGAAAAGLEALVRERSAGRDPNEAASREL
                     AEEIRRGLEDISHLMRG"
     gene            complement(11108..11941)
                     /locus_tag="VFU20_08950"
     CDS             complement(11108..11941)
                     /locus_tag="VFU20_08950"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006967396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HEU5286615.1"
                     /translation="MDDLVSTRWLADNLGAPDLVVLDASLFMPASGRDARAEFLAAHI
                     PGARFLDIDEQSDKANPAPHMLPPAEEFGSAMEALGVGRGDRIVVYDNSPLRSAARGW
                     FMLRHFGTERVAVLDGGFEKWLAEGRPVESGEPPPRPARFAAIERPGEVVSKDEVRAG
                     LDAVLLDARGQGRFEGSEPDPRPGVAAGHIPGARNLPFAQLYNEDGTLRARGELARAF
                     ATAGADPSAPFVASCGSGVTASSLIFAAHLLGNCETRLYDGSWSEWGADPSTPKATGP
                     A"
     gene            11989..12435
                     /gene="queF"
                     /locus_tag="VFU20_08955"
     CDS             11989..12435
                     /gene="queF"
                     /locus_tag="VFU20_08955"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HEU5286616.1"
                     /translation="MDPKHLGKASALPASPEEAELDYVPNPRPGTLYLVRFAAPEFTT
                     LCPVTGQPDFAHLVIDYAPAETIVESKSLKLFLSSFRNHGGFHEDVTVGIGQRLFDEM
                     KPHWLRIGGYWYPRGGMPIDVFWQTGTPPDGLWLPDQGVAPYRGRG"
BASE COUNT         1943 a         4267 c         4189 g         2042 t
ORIGIN      
        1 acgggaaggt cgcccggctg ctgcgcacct gcctcaaata gtggtcattc ggtcggcggc
       61 aggcccggcg cgggagccga cggcggggcc gcgccctcgt cgacttcata atcgctcatg
      121 tagcgcttca tcccctcgcg ctcgccatat tcggtgagcc acaaagcgag gaagccgtag
      181 ttgaaggcga ccgacatcac caccgcgagc tcgaccgcgc cgatcccggc cgacaggccg
      241 atgccgaccg acagcaggat gaacagggcg tcgcccgagc tcttgagcga gttcttgaag
      301 cgcaccgcgc cggcgatccc ggcgagcgag aaggccagcg ccagcgaatt ctggacgatg
      361 acgacgatgc cggtgacgac caggggcagg atgatgatcg tgctgatcag cgactggtcg
      421 tactcgtcgc cgtggcggat cgccatgtag gtccagctga ccggcagcgc ggtcagcagc
      481 gcgccgacga tcgcggtggt caaccagaac aggctttcgc ccaagttggc gacattctcc
      541 gtctgctgga cctggacccc ggtgaggccg ttggcgccgg cctgctgcgg ctggttgatc
      601 agctcctcga cgccgccgac cgggagatag tcgcggaccc acgggaacag cgtcagagcg
      661 ccgtagacca gcaccgcgat gaccagataa taagcgctca actgagcgaa caaccgcaac
      721 gccttcatcc ctgtcccctc gaccggccgt cggtccgcgg cgcccaagct acgcttttcg
      781 cagcgctccg caatcgcgtt tcaagcctgg tagttgggaa tcggcctcag caccccgaac
      841 cgctccttgc ccggcggccc gggcagcggc ggccacggga tctcgttcaa tggcagctcg
      901 gtgtcgggga tccggttgag caggtcgcgc agcagggtca gccggcccag cgcctggtcg
      961 ttgaaatcga ccagcgtcca cggcgcgaag tcggtgtggg tcgcggcgag catcttctcg
     1021 cgcgcttcgg tgtaggcgtc gtatttgctg cgcgcctcga tatcgatcgg cgacaatttc
     1081 cagcgcttga gcgggttgcg aagccggttc tcgaaccggt gctcctgctt gtcctggtcg
     1141 cagcacagcc aatatttgaa cagcaggatg ccgtcgtcga ccaggtggcg ctcgaactcc
     1201 ggcaccgcgc gcaggaaggc atcggtctcg gcttgcgagc aatagcccat caccggctcg
     1261 accccggcgc ggttgtacca gctgcggtcg aacagcgcga tctcgcccct gggcggcaga
     1321 tgcggcgtgt agcgctggaa ataccattgc gcgcgctcac gctcgttggg cgaggccaaa
     1381 gcgacgacgt ggcactggcg cgggttgagg acccgggcga tcatgtcgat cgagccgccc
     1441 ttgccggccg tgtcgcgccc ctcgaacagc acgacgatcc gctgcccggt ctcggcgacc
     1501 cagcgcgcca tcccgctcag ctccttggtc agcggcttga gcagccgctc gtattcgtcc
     1561 tgactgagct cggccatgcg tcggatcccc tatggccata gtggcaaggc cgaccagcgt
     1621 cggcaatggg tagaaaacag gcccgcgcgg gcgatttgac cggtgcaact cgcgcccccg
     1681 agtcgcgccg ccttcgccga gtcctgattc gtgacatgtc aagaattttt gcaaccgccg
     1741 cgccgaacgg gcgttggagc accaaaggct tttaagaaaa gagaaaaacc gtgttcctgc
     1801 ggaccctgtc ccgcggcgcc cttgctgcgc tgctgctttc cgccgtgccc gtcgtggccc
     1861 cgaccgccgc cgtcgcggcg agcccggccg cgggtctggc ggtgccggcc gacttcggct
     1921 cgccggtcta tcccaagcgt ggccgctacc tgctggtcga cgccgcctcg gccaagctgt
     1981 tcatggtcga ggacggccag gtggtcgaca cgatgcgggt gatcgtcggc aagcccgacg
     2041 cccagacccc gaccatcgcc agcaagatct attacgcgac tctcaatccc tattggaacg
     2101 tgccggccga cctagcgcgc aagatcatcg ccccgcgggt gctcgaaaag ggcgtcggct
     2161 atctgaagca gaacggctac caggtgctgg cgagcttcga cgacggggcg cccgagatct
     2221 cgccccgcga ggtcgactgg aaggccgtcg ccgccggcgc cgagacggtc aagatccgcc
     2281 agctgccggg ccccggcaat tcgatgggcc aggtcaagtt cggcttcccc aacggcttcg
     2341 ggatcttcct tcacgacacg cccaagaagg agctgttcgc gatgcccgag cgcgcgatca
     2401 gcaacggctg cgtgcgcctg gaggacgcgc ccaagctcgc ccgctggctg ctcggccgcg
     2461 acccgagcgc gatcgtcgcc tcgtccgagc ccgagcagca cgtgccgctg ccgcgcgcgg
     2521 tgccgatcta catcacttac ctggaccggc agccggccga gctcgcgctc gcgcccagca
     2581 ccaccagcag cccgcttacg cgctagggct caggccgcga tttcctcgtc ggtcatcacc
     2641 gggcccgaat cctggccctt ggcggcgggg tcgcggtcga cgaactcgat gatcgcgatc
     2701 ggcgccgcgt cggacgcgcg gatgcccgcc ttgatgatcc gggtgtagcc gccggcgcgg
     2761 cccgcgtagc gcggcccgat gacatcgaac aatttggcca gctgcgcctc atccatcagc
     2821 cgcccctgag cgagccgcct gttcgacagc ccgcccttct tggccagggt gaccagtttc
     2881 tcgacgtagg gccgcagctc cttggccttg gcggtggtgg tggtgatctg ctcgtgcttg
     2941 atcagcgccg ccgccatgtt gcggaacagg gcggtgcggt gggagctggt gcgctgaagc
     3001 ttacgatggc cgacgcgatg ccgcatgata cttccttcat gttcgttcag gggccgttcg
     3061 aggtaccccg gaccaggcgg tgaaaacggg gcaccgccca aaccccgacc aggcgtttag
     3121 ccgagcattt cctgctcgag cttcttggcc atttcctcga tgttctccgg cggccagccg
     3181 gggatgtcca tgccgagccg caggcccatc gacgccagca cttccttgat ctcgttcaag
     3241 gacttgcggc cgaagttggg agtgcggagc atctccgcct cggtcttctg gaccaggtcg
     3301 ccgatgtaga tgatgttgtc gttcttcagg cagttggcgc tgcgcaccga cagctcgagc
     3361 tcgtcgacct tcttcaagag atagcggttc aattggttgg tgtcggcctg ggcctcgacc
     3421 ggggtcggca tgccgcccac catcgccggc tgcggcgccc ggatcgcgcc ttcctcgaag
     3481 tggacgaaca gttgcagctg gtcctggagg atccgcgccg catagcccaa ggcgtcctcg
     3541 gggctgatcg tgccgtcggt ctcgatggtc agggtgagct tgtcgtagtc gagctcctgg
     3601 ccgacgcggg tgttctccac cttgtaggcg acctggcgca ccgggctgta gagcgcatcg
     3661 acggggatca ggccgatcgg cgcgtcggcc gggcggttgg cctggctcgg cacatagcct
     3721 ttgccgacgt cggcggtcag ctccatgttg agcgtcgcgc cttcgtcgag gtggcagatc
     3781 agctggtcgg ggttggtgac ctcgatgtcg ccggtggtcg cgatcatccc ggcggtgacg
     3841 tcggtcgggc cggtcgccga aagctgcagc cgcttgggcc cctcgccttc catcctgagc
     3901 gcgatctgct tgacgttgag gacgatgtcg gtaacgtcct cgcgcactcc ggtcagcgac
     3961 gagaattcgt gcagcacgcc ctcgatcttg atcgaggtga tcgcggcgcc ctgcagcgag
     4021 ctcagcagca cccgccgcag cgagttgccg agagtcatgc cgaagccgcg ctccaatggc
     4081 tcggcgacga acgaggcgcg gcggcgggcg tcgccgccga tcttgcgctc gagggcgttg
     4141 ggcttcttga gttcctgcca gttctttgcg ttgacggcca tttgtcttcc tgcccttggg
     4201 cggggagctc cccgccggta aaatctacaa aaccagagcg gccgcggcgg gccgcccatc
     4261 gcatccgatc agacgcgcct gcgcttgctc ggcctgaccc cgttgtgcgg gatcggggtg
     4321 acgtcgcgga tcgaggtgat ctggaagccg accgcctgca gcgcccgaag cgcgctctcg
     4381 cggcccgacc cgggaccctt gacctcgacc tcgagcgtcc ggacgccatg gtcggcggcc
     4441 ttcttgccgg cgtcctcggc ggcgacctgg gccgcgtagg gcgtcgactt gcggctgccc
     4501 ttgaagccca tcatgccggc gctcgaccag gcgatcgcat tgccctgcgc gtcggtgatg
     4561 gtgatcatcg tgttgttgaa gctggcgttg acgtgcgcga caccggcggt gatgttcttg
     4621 cgctcccgcc gacggaggcg ctggggtgct tgggccatgg gtcctacgat cctctaaatc
     4681 cgtttgccct tgtttcttcc gaagcccgga agggcttgtc gaagggctgt cctgttgctg
     4741 ccgagcgaga agggtctcgc gaccatcccc cggatgatcc ctcgcttact tcttcttccc
     4801 ggcgatcggc ttggccttgc ccttgcgggt gcgcgcgttg gtgtgggtgc gctggccgcg
     4861 gaccggcagg cccttgcggt ggcgaagccc ccggtagcag gcgaggtcca tcaaccgctt
     4921 gatgttcatc gccgtctcgc gcctgaggtc gccctcgaca ttgtggtcgg agtcgatcgt
     4981 ctcgcggatg tggagcactt cctggtcgct caggtcctgg acccgctttt ccggcccgat
     5041 cccgagcttc tcggtgatct gcctggcctt gctccggcca attccgtgga tgtaggtcag
     5101 cgcgatctcg acgcgcttgt tggtgggtat gttaacgccg gcgatacggg ccattctcga
     5161 tcctctttta gctccacggg cggggaaatt ccgcgcccat ctcctcgcta gtcacccggt
     5221 ttcaagcgaa aaaccgacgc gcgcacgaat gcgccgccgg gaaccggttg atcgggatga
     5281 gttgcaaata ggaatatgac ggggccgagt caagcgaagc gggagcagcg attgccgcgg
     5341 cccggacagc cggatagagc cgcccgcacg atggagcggc tgacccccga tcaacccctg
     5401 cccgagcggc ttcgcgccgg catcgtcgcg ctcggcaatt tcgacggctt ccacctcggc
     5461 caccaggcgg tcgtcggccg cgccgtcgcc cgcggccacc acgagcggcg gccggtgatc
     5521 gtcgccacct tcgacccgca cccggtgcgc ctgttcaagc ccgaggtgcc gccgttccgg
     5581 ctgaccagcc tcgaccagcg cgagcggctg ttcgcccatg ccggcgccga cgcgatgctg
     5641 gtgttccgct tcgaccagca gctcgccgcg accagcgccg aggatttcgt cgccgagctg
     5701 cttggccggc ggatcggcgc ggccggagtc gtcaccggcg acgatttcac cttcggcaag
     5761 ggccgcgccg gcaattccga aacgctccgg tcgctcgggc tcagggtcgg gatcgccgcc
     5821 gaggcggtgc ctccggtgct gctcgacggg acgcggattt cgtcggggcg gatccgcgag
     5881 gcgctcgccg ccggcgaccc cggcaccgcc acccggctgc tcagccggcc gttcgcggtc
     5941 gaggcggtcg tcgagcgcgg cgacggccgc ggccgcgacc tcggctatcc gaccgccaat
     6001 ctgcggctcg gcgactatca gcggccggcc tacggcattt acgcgatgcg cgtgcgcctc
     6061 gacgacgagc gcgagtttcc cggcgtcggc agcctcggag tgcgcccgac cttcgagccg
     6121 cccatcgagc tgctcgaggc gcatctgttc gacttcgacg aggacctcta cggccggacc
     6181 atcgaggtcg cgctccacgc gttcatccgg ccggaggaga agttcgccga cgcggcggcg
     6241 ctggcggcgc ggatgaagga ggacgaggcc gaggcgcgga agctgctggc gctcgcctag
     6301 cgttgcattc ccccacccgc ccgctagagc gcgcagccaa tgtccgacgc ccccgaaaaa
     6361 cccaattacc gcgagaccgt gttccttccg aggaccgatt tcccgatgaa ggcgggcctg
     6421 ccgcagaagg agccggcgat cctcgcgcgc tgggccgatc aggacctcta cggccaggtc
     6481 cgcaaggccc gcgcgggccg cgacaagttc atcctccacg acggcccgcc ctacgccaac
     6541 ggcgacatcc acatcggcca cgcgctcaac catgtgctga aagacatggt ggtgcgcacc
     6601 cagacgctgc tcggcaagga cgcgccctac gtgcccggct gggactgcca cggcctgccg
     6661 atcgagtgga agatcgagga gcagtaccgc aagaagaagc tcgacaagga cgaggtcccg
     6721 ctgaccgagt tccgggccga atgccgcgcc tatgcgcagc attgggtcga gacccagcgc
     6781 gagcagttga agcggctcgg gatcggcggc gactgggaca acccgtattt gacgatggac
     6841 tatgaggccg aagcgacgat cgtcgccgag ctgatgaagt tcgccgaaag cggccagctg
     6901 taccgcggcg ccaagccggt gatgtggtcg ccggtcgaaa agaccgcgct cgccgaggcc
     6961 gagatcgaat atgaggacgt ggtctcgacc cagatcgacg tcgcgttcga gatcgtcgag
     7021 agcccgatcc cggagctggt gggcgcgtat gcggtcgcgt ggaccaccac tccgtggacg
     7081 atccccgcca accaggcgat cgggtacggg ccggaaatcg agtacggact ctatgagatt
     7141 tggctgaacg tttcagacgc cggtgacggt gatctgtcgc tcgaacaacg acgcaacccg
     7201 ttaatggaca cgctggataa gcggcgaatt ttgatagcga agaaccttaa agaggcattc
     7261 aaggagcgac tcaaccgcgg cctcaaggac aagagcacgc aagcagcagt gcagctatcg
     7321 gccgcgttaa aggatttgca gtccttgcac ggcttgggat tggctggctc tatcgcccgc
     7381 cacccgatgc accatctcgg cgggttcttc gcccggccgc gcccgttcct cgcggcgccg
     7441 ttcgtcacca ccgaccaggg cacggggctc gtccacgagg cgcccgacca tggcgaggag
     7501 gatttctacc tgtgccagca gcacggcatc gacccggtgt tcgccgtcac cgacgacggc
     7561 cgctaccgcg acgactgggc gtggctcggc ggccagggca gcgtcatcaa cgccaagttc
     7621 aacgcgcccg acgggccgat ctgctcggac ctgcgggaaa cgggcgcgct gctcgctgcc
     7681 tccgcggact tcacgcacag ctacccgcac tcgtggcggt cgaaggccaa ggtcatctac
     7741 cgctgcaccc cgcaatggtt catcgccatg gaccggccgc tggacagcgg aagaaccctc
     7801 cgcgaaaccg ccgtgcaggc gatcgccgac tcccgcttcg tgcccgggaa gggccgcaac
     7861 cgcctgggct cgatggtcgc cgagcggccc gactgggtga tcagccgcca gcgcgcctgg
     7921 ggcgtgccga tcgctttgtt cgtcgagagg aagaccggcg agctgctggt cgatcccgag
     7981 gtcaataagc ggatcgtcga ggcggtgcgc gccggcggcg tcgacgcgtg gaacgacgcc
     8041 aacgccgcgc agctgctcgg agccgggcgc gacccggccg attacgagcg tcagaccgac
     8101 atcctcgacg tctggttcga cagcggctgc acccacgtgt tcacgctcga aagcgggcgc
     8161 tggcccgatc agcgctggcc ggccgacctt tacttggaag gctcggacca gcaccgcggc
     8221 tggttccagt cgtcgctatt ggaaagctgc ggcacccgcg gccgggcgcc ctacgacgcg
     8281 gtgctgaccc acggcttcac gatggattcg aagggcatga aaatgtccaa gagcctcggc
     8341 aacgtcatca acccactcga cctgatgaag gagcatggcg ccgacatcct gcggctgtgg
     8401 gcgctgtcgg tcgacttcac cgaggaccac cggatcggca aggagatcct cggcggcgtc
     8461 gccgaccagt accgcaagct ccgcaacacc ttccgctatt tgctcggcgc gctcgagagg
     8521 ttttcggacg acgagcgggt cgcggacgtg accgcgatgc ccgagctcga gcgctacatg
     8581 ctgcacctga ccgccgaact cgacgcgaag ctaagggccg cggtcgggga gttcgacttc
     8641 aacacctacg tccgggcgat caccgacttc tgcaacgaag atttgtcggc cttctatttc
     8701 gatatccgca aggacgtcct ctactgcgag gtcaacccgg cgaccggccg gcagaccgac
     8761 aagcgccgct cctaccggac cgtgctcgac accctgttcc acgcgctggt gcggtggctg
     8821 gccccggtgc tggtgttcac gaccgaggaa gtgtggggca cgcgctttcc tgacggcggc
     8881 tcggtgcatc tgctcgaatg gccggagatc cccgccgtgg cggcggacga cgaaaaatgg
     8941 gcgaagctgc gcgagcttcg gaccgccgtc accgaggcga tcgagccgct gcggcgcgac
     9001 aaggtgctcg gctccagcct cgaggccgag gtgaccttgc ccgaggccga cgcgtcgctc
     9061 gacctcgccg agctgttcat cgtctcggcc gtccagcccg gcgacgggct gaaggtcgcg
     9121 aaaaccgcca accgcaaatg cgggcgctgc tggcgacatt tgcccgaagt gtccgaggac
     9181 ggcggcctgt gcgcgcggtg cgaggaggtc ctcaatggct gagcgccgct tcggcttcgg
     9241 catcgcgctg gtggtcttcg cgctcgacca gctgaccaaa tggctgatga tcggcccgct
     9301 cgagctgcag tccgtccggc agatcgtgct gctgccgatc ttcaacctga gctggaccga
     9361 gaattacggg atctcgctcg ggctgctcaa cgccgagacc gaggtcggcc gctggatgct
     9421 ggtcgcggtg accgcggcga tcgccgtcgg ggtcgcgatc tggatcggcc gcgagaagca
     9481 gcgcggcgac cggctcgcgc tcgggatggt gctcggcggg gcactcggca acatcctcga
     9541 ccgggtccgc ttcggctatg tggtcgactt cgccgacctg cacttcggca ccttccgtcc
     9601 gtttttggtc ttcaatgtcg ccgacgcagc gattagcatt ggcgtcgtca tcctgttact
     9661 gcgcgccttc ctcatccggg atgagcggcc gaaggagaat atcgaacatg cgtaattgga
     9721 actccgcgct gctgcttgtg ccggcgctcg ccctgggcgg ctgcgccatg ctcggcggcg
     9781 gcaaccgcgc gccggacgag ttcgcggtgg tccgcaacgc gccgctgatc atcccgcccg
     9841 actacacctt gaccccgccg gtcgcgggca ccgccgcggt cagcccggcc gaggcccagg
     9901 cgcaggcgat cgaagccctg ttcggcggcc cggcaccgcg cagcaatgcc gaaaccagcc
     9961 tgctcgagcg ggccggccgc gaccgcgccg cggtcggggc gcggtccagc gtcggcgatc
    10021 ccaatacgcg ggtggtcgac aagggcacgg taacggcgca gattctggtg gcgccgcaag
    10081 ccgacggccg cgacgcgacg gtccaggttc caaggtagca ggcagaccgg ggggagcgag
    10141 cgcaaccatg gctgaagaat cgctttccgc gcgcctcgcc gaggtcgaat cgatgatcga
    10201 ccgctcgcgc cgccgcttcg aggaagggtc gcggctggtc gaggaggcgc acaaggcgct
    10261 ggccgacctc cagcaggaat tgatcggctc gccggtcact ttgtcgcccg aggacagcga
    10321 ggaagcgtcc tcgcggctgc tgacgtcgat caaggcggcc ggcggcgaga tgcccgagac
    10381 cttgtgcggc ggccgactgt cggtcgacca ggtgcagcgg ctgatccgga tcgacggcca
    10441 cccgatcggc atcaccgaga tggaatatcg ggtgctcgag ctgctcgcct tcgcccgcaa
    10501 caacgtcgtc acccgggcga tgctcctcaa gcacctctat cgccgcgccg acgaccagcc
    10561 gcagcccaag atcatcgacg tgttcatctc caagctcagg aagaagctgc ggatggcgtc
    10621 cagcggccat gagttcatcg agaccatccc gcagcgcggc tggatcctgc gcgacatcga
    10681 ggccggatcg ggcgcctagc ccgaaagccg cgattcatcc tttgttagcc ttacgggcat
    10741 agcccgggcc gatgctgaat ttcgtttccg tcccgcaggc cgacgatccg ccggagccct
    10801 atgccgaggc gctggcgcgg ctggcgagca tccgccacgc gcttcggcta gtcgacccgt
    10861 tcggcggcgg gccggcatcg gacccgtccg aggacgacgc gatcgcggcc gcgtggaccg
    10921 gcgccggcaa ggcggccgcg cgctgcttcg acggccgcac ggcccgcacc gcgggcgcgg
    10981 ccgcggccgg cctcgaggcg ctggtccgcg agcgttcggc gggccgcgac ccaaatgagg
    11041 cggccagccg cgagctggcc gaggaaatcc gccgcggcct cgaggatatc tcccacctga
    11101 tgcgcggcta ggccggcccg gtcgccttgg gcgtcgatgg atcggcgccc cactcgctcc
    11161 agctgccgtc gtaaagccgg gtttcgcaat tgccgagcag gtgcgcggca aagatcagcg
    11221 agctggcggt gacccccgag ccgcagctgg cgacgaacgg cgcgctcgga tcggcgccgg
    11281 ccgtggcgaa cgcccgggcg agctcgccgc gggcgcgcaa ggtgccgtcc tcgttgtaaa
    11341 gctgggcgaa cggcaggttc cgcgctcccg ggatgtggcc ggcggccacg ccaggcctcg
    11401 ggtcgggctc gctgccctcg aaccgtccct gcccgcgcgc gtcgagcaac acagcgtcga
    11461 ggccggcgcg gacctcgtcc ttgctgacca cttcgcccgg ccgctcgatc gccgcgaagc
    11521 gggccgggcg cggtggcggc tcgccgcttt cgaccggccg cccctcggcc agccattttt
    11581 cgaagccgcc gtccaggacc gcgacccgct ccgtcccgaa gtgccgcagc atgaaccagc
    11641 cgcgcgcggc gctgcgcagc ggcgaattgt cgtagacgac gatgcggtcg ccgcggccga
    11701 ccccgagcgc ttccatcgcg ctcccgaact cttccgctgg agggagcatg tgcggcgcgg
    11761 gattggcctt gtccgactgc tcgtcgatat cgaggaatcg cgccccgggg atgtgcgcgg
    11821 cgaggaattc ggcgcgggcg tcgcggccgc tggccggcat gaacaggctc gcgtcgagca
    11881 ccaccaggtc gggcgcgccg agattgtccg ccagccaccg cgtcgagacc aggtcgtcca
    11941 tggcgccagg ataggcttgg gcgcgctgcg ctgctaggtg gcgcggcgat ggaccccaag
    12001 catctcggca aggccagtgc cctgcccgcc tcgcccgagg aggccgaact cgactatgtc
    12061 cccaacccgc ggccgggcac gctgtacctg gtgcggttcg cggcgcccga gttcacgacc
    12121 ctgtgcccgg tcaccggcca gcccgacttc gcccatctgg tgatcgacta tgcgccggcc
    12181 gagacgatcg tcgaatcgaa gagcctcaag ctgttcctca gctcgttccg caaccacggc
    12241 ggcttccacg aggacgtcac cgtcggcatc ggccagcggc tgttcgacga gatgaagccg
    12301 cattggcttc gcatcggcgg ctattggtac ccgcgcggcg gaatgccgat cgacgtcttc
    12361 tggcagacgg gcacgccgcc cgacgggctg tggctccccg accagggcgt cgcgccctat
    12421 cgtgggcgcg gctagcggta c
//
LOCUS       DASTZO010000317         9719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_411940,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000317 DASTZO010000000
VERSION     DASTZO010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 9719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9719
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411940"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..490
                     /locus_tag="VFU20_08960"
     CDS             224..490
                     /locus_tag="VFU20_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286617.1"
                     /translation="MNRPGDRMGGAERARWLAELAQAVDQAQQLAWSLGASKANAAEV
                     EGLYRRLEAVRIEIEALRRGRFPVREADTDPIWTSLLGWNGRPG"
     gene            complement(487..1311)
                     /gene="cysQ"
                     /locus_tag="VFU20_08965"
     CDS             complement(487..1311)
                     /gene="cysQ"
                     /locus_tag="VFU20_08965"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013933809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HEU5286618.1"
                     /translation="MDELGLLDDIAVAAREAGEAIVAIVRRGFEVEAKRDRSPVTEAD
                     RAAELIILAALARTAPGVPVIAEEEVAAGRIPAHGDTYFLVDPLDGTKEFVRGGDDYT
                     VNIGLIVGGSPRLGVVFAPATGALHGGLVGMGAWVEEGGGRRPIAARTPGSELVAVAS
                     KSHFNQATADYLDHSASGCGHVAVGSSLKFCIVAEGRADIYPRLSPTSEWDTAAGHAV
                     LLAAGGRVDGPDGAPLRYGKRAFINRGFVATGGWPAPAIGPWLAEFAGGGDLPEGV"
     gene            complement(1314..2093)
                     /locus_tag="VFU20_08970"
     CDS             complement(1314..2093)
                     /locus_tag="VFU20_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007688154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5286619.1"
                     /translation="MDIQGISAVVTGAASGLGEATARELARRGAKVAVFDRDAEKGER
                     VAAEIGGLFCEADVTSDEKVAAAFAKAREAHGQERVLVNCAGVANAAKTVGRDKETGA
                     IKFYPMQQFELAIAINLVGSFRCIAHAAAGMVALDPLEDNERGCIVNTASVAAQDGQI
                     GQAAYSASKGGVLAMALPIARDLMNDGIRVNTILPGVFKTPMVAMMPSQVQEALGAQV
                     PFPKRLGKPEEYAQLACFMVESRYMNAEAVRLDGGIRMAPR"
     gene            2328..3647
                     /locus_tag="VFU20_08975"
     CDS             2328..3647
                     /locus_tag="VFU20_08975"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HEU5286620.1"
                     /translation="MKSRFLRSFACATMLVPVVGATAASAQAIVPVAVVQPASLPSAG
                     AVTAFYNTYRSAPMWFRGGAANPAAGKLVSILRRAQFDGLASGPQLASQVEAAITRAA
                     SGSPSDVMAAEQILSAAWVMYVQALKRPTPRMIYAYPVLAPQNTRPDQILLTAAAAPS
                     LEAHVNAVSSINPTYAQIREAAWLQAQASGTGPDPRLLANLERARPLPAGGRYIVVDS
                     ATQRLTMYENGQPVDSMKVVVGTRDLPTPLIASMIHYVTFNPYWNVPHHLVRKTVAPN
                     VLKQGLGYLKSRGYEVMADWSEEAAVLPADSVDWKAVAAGKTQIRVRQKPGPGNSMGE
                     MKFRFPSGEDIYLHDTPTREYFAKSQRTLSNGCVRLEDAKRLARWLMGREPLAPSKDP
                     EIRVQIPQGVPIYLTYLTAQPSSSGVTYLSDVYGWDQPAGQQVASSK"
     gene            complement(3802..4683)
                     /locus_tag="VFU20_08980"
     CDS             complement(3802..4683)
                     /locus_tag="VFU20_08980"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU5286621.1"
                     /translation="MRKLAIGMALASTMLATPAVARDGSLYAGIEGGLMLVEDLNLDY
                     SDEGLFPFQPSEIEDAVIVKHKTGFDIDGIVGYDFGMVRVEGELAYKRASVDELELSP
                     FISDEGPGDNFDADGRATAISAMVNLLLDFGDDDGWSGYVGPGFGLARVKYRADADEV
                     GISFRDTDSTLAWQVIAGVRTALSPNIDLGLKYRFFNTGRIKFHDEDAGSGVVGGVTP
                     VDLIGKWRSHSLLASLIYNFAAPPPPPPPPPPPPPPPPPPPATQTCPDGSVILATDVC
                     PVPPPPPPPPPPEPERG"
     gene            complement(4841..5263)
                     /locus_tag="VFU20_08985"
     CDS             complement(4841..5263)
                     /locus_tag="VFU20_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013846189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2793 domain-containing protein"
                     /protein_id="HEU5286622.1"
                     /translation="MAGQAQKDLFHNEALQVLDAVVAAAVEGLPQAAPPASPAVGSGY
                     IVGSSYIVGSSPSGDWAGHANQLAAYTSGGMAVYRNGSWEIGKLRGSEVIVDGQKVVG
                     ARGATIASPAGGTMVDTEARAAIGEILAAMREHGLIAS"
     gene            complement(5352..6395)
                     /locus_tag="VFU20_08990"
     CDS             complement(5352..6395)
                     /locus_tag="VFU20_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU5286623.1"
                     /translation="MPLPRLFRLASRAIAIDLGTANTVVYVQGRGIVISEPSVVAMEV
                     LNGVRRVKAVGADAKLMMGKTPDYIKTIRPLRNGVIADLEVAEQMIKHFVEKAQGGRS
                     RLSRRPEIVLCVPSGSTSVERRAIRDAASHAGASQVSLIDEPMAAAIGAGLSVTDPVG
                     SMVVDIGGGTTEVGVISLRGLAYSQSARIGGDKMDEAISSYVRRNYNLLIGEATAERV
                     KKEIGTAKPPESGKGLVAYVRGRDVAKGVPSEISLNQAEIADALAEPVARIVHIVRVA
                     LEHTQPEIAADIIDEGITLTGGGSLLRGIEEVLADETGLPVRVAEDPMSCVALGAGRT
                     LEDPDYRGALHPV"
     gene            6714..7157
                     /locus_tag="VFU20_08995"
     CDS             6714..7157
                     /locus_tag="VFU20_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HEU5286624.1"
                     /translation="MTLPRTLFRIVAPLALLASAEGAVAATLLVTKTAFCGCCRIWVE
                     RMKAAGFTVKVNDVENVTPTARRLGVPDKLRSCHTAEIGGYAIEGHVPAADIKRLLAQ
                     KPKAAGIAVPGMVAGTPGMEQGGHSEPYQVILFDRAGKTKVFASH"
     gene            complement(7201..7716)
                     /locus_tag="VFU20_09000"
     CDS             complement(7201..7716)
                     /locus_tag="VFU20_09000"
                     /inference="COORDINATES: protein motif:HMM:NF019896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase family
                     metallohydrolase"
                     /protein_id="HEU5286625.1"
                     /translation="MLKVLAGLALAAGAAPAWAASQPTDFGVRAVSGEAALASEPAAA
                     ASVATAAAVPGGRISLTAAASLGSQWGRVTSTWRSREHNRRVGGVRNSFHLSGRAIDI
                     ARRPGVSHAQIAAAFRNAGYRLIESLDEGDHSHFAFGTGAAAPSYRPLQQQRTAATQW
                     RVVTAASAILK"
     gene            7885..8184
                     /locus_tag="VFU20_09005"
     CDS             7885..8184
                     /locus_tag="VFU20_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286626.1"
                     /translation="MPRRVKDFIEISEYTSLDDLIRFLETIRATLPPDSEAELKIRGD
                     EVFGRRLTITFLRGLTDEEASLEARYTGDVPPEPDSAIEQLRAKLDKVPYRDSGR"
     gene            complement(8181..9416)
                     /locus_tag="VFU20_09010"
     CDS             complement(8181..9416)
                     /locus_tag="VFU20_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286627.1"
                     /translation="MNESVSTVAAFPELFGVPVEFVLFGLMLAAIAAFHRHVLPIAAA
                     GLVAILAYQGLVTQFPTGPGLGALGEHLAHEWVTLTNLLLLLVGFEILSNQFEQSNVP
                     DHLPRLLPDGWTGGLVLLAMVFVMSMFLDNIAGAVLGAVIARHVYKGRVGVGFLAAIV
                     AAANAGGAGSVIGDTTTTLMWLHGVSPIAVLPAYAAAIPAFAVFAVAGSWAQHRHQPI
                     LAHNEPDHPLQWRRITIVGVILAAAVAANIWANATGVGEAAPWLGLAIWGAILATSPA
                     GPPEWSVAKPAAQGALFLVLLVAAASLMPVDSLPSPGWESVAALGFLSAVFDNIPLTM
                     LALKQGGYDWALLAYAVGFGGSMMWFGSSAGVAVTVMYPEARSAWRWLKEAWFVPIAY
                     VVGFAAQMLLIGWVPENRG"
BASE COUNT         1519 a         3503 c         3211 g         1486 t
ORIGIN      
        1 gcacaaacac aatcgcggca attagagggc cgagcaccgg ctaaacctca gccgcacctc
       61 ccttgggatg cactatgctg ttgcatgtta catgtcccaa ggggatatgt gccacgcgca
      121 tagtggagaa aagtcaggcg attttggctc cgcaacggat gtttcgccaa ccgggcgctc
      181 atagcgcgtc gctaagcacc cgctgatcga ggaggtgaat gcggtgaatc gacccggtga
      241 ccgaatgggc ggagccgagc gcgcccggtg gctggccgag cttgcgcagg ccgtcgacca
      301 ggcccagcag ctggcgtgga gcctgggagc ctcgaaggcg aacgccgccg aggtcgaagg
      361 gctgtatcgg cggctggagg cggtgcggat cgagatcgaa gcgctgcgcc gcggccgctt
      421 tccggtccgc gaggcggaca ctgatccgat ctggaccagt cttctcggtt ggaacggacg
      481 gccgggctag acgccctcgg gaaggtcgcc gccgcccgcg aattccgcaa gccagggccc
      541 gatcgccggc gccggccagc cgccagtggc cacgaagccg cggttgatga acgcccgctt
      601 gccgtagcgc agcggcgccc cgtcgggccc gtcgacccgg ccgccggccg ccagcagcac
      661 cgcatggccg gccgcggtgt cccattcgct ggtcggcgac agccgcgggt agatgtcggc
      721 ccggccttcg gcgacgatgc agaatttgag cgacgagccg accgcgacat ggccgcagcc
      781 gctggcggaa tggtcgagat agtccgcggt cgcctggttg aagtgcgact tggaggcgac
      841 cgcgaccagc tcgcttcccg gcgtccgggc cgcgatcgga cggcggccgc cgccctcctc
      901 gacccacgca cccattccga cgagcccgcc gtgaagcgcc ccggtggccg gcgcgaacac
      961 cacccccagc cgcggcgagc cgccgacgat caggccgatg ttgaccgtgt aatcgtcgcc
     1021 gccgcggacg aattccttgg tgccgtcgag cgggtcgacg aggaaatagg tgtcgccgtg
     1081 cgccgggatc cggccggcgg ccacttcctc ctccgcgatc accgggacgc ccggcgcggt
     1141 gcgggcgagc gcggcgagga tgatcagctc ggctgcccgg tcggcctcgg tcaccggcga
     1201 gcggtcgcgc ttggcctcga cctcgaagcc gcgccggacg atcgcgacga tcgcctcgcc
     1261 cgcctcgcgg gcggcgacgg cgatgtcatc caataggccg agctcgtcca agatcagcgc
     1321 ggcgccattc ggatgccgcc gtcgagccgg accgcctcgg cgttcatgta gcgcgattcg
     1381 accatgaagc aggcgagctg cgcatattcc tcgggcttgc cgagccgctt ggggaacggc
     1441 acctgcgctc ccagcgcctc ctgcacctgg ctcggcatca tcgccaccat cggcgtcttg
     1501 aacactccgg gcaggatggt gttgacccgg atcccgtcgt tcatcaggtc gcgcgcgatc
     1561 ggcagcgcca tcgccagcac cccgcccttc gaggccgagt aggcggcctg gccgatctgg
     1621 ccgtcctggg cggcgaccga ggcggtgttg acgatgcagc cgcgctcatt gtcctcgagc
     1681 gggtcgagcg ccaccattcc ggccgcggcg tgggcgatgc agcggaagct gccgacgagg
     1741 ttgatcgcga tcgccagctc gaactgctgc atcgggtaga atttgatcgc ccccgtttcc
     1801 ttgtcgcggc cgacggtctt ggcggcgttg gcgacgcccg cgcaattgac cagcacgcgc
     1861 tcctggccgt gcgcctcgcg cgctttggcg aacgccgccg cgaccttctc gtcgctggtc
     1921 acgtcggcct cgcagaacag gccgccgatc tccgccgcca cccgttcgcc cttctccgcg
     1981 tcgcggtcga acaccgcgac cttggcgccg cgccgcgcaa gctcgcgcgc cgtcgcctcg
     2041 ccgagccccg aggccgctcc ggtgaccacc gccgatattc cctggatgtc catgttcgcc
     2101 cttcaatgcg ccagatgacg cggggcctat gctatggaat tgggcaatca ccaagagggg
     2161 cgtcgccgcc tcgaaagtgg tgcccggggg acacattgcg gcgccagaag cggaccaatc
     2221 atctggaaaa tggctcgaag tcgctgacat tgcggtcgcg acgtggcaat tttccgagcg
     2281 cacgcccata cgggcgctat tcatcttcgc gtcgctagag gacgggcatg aaatcgcggt
     2341 tccttcgatc cttcgcttgc gcgacgatgc tggtgccggt cgtgggtgcg accgccgcgt
     2401 ccgcccaggc aattgttccg gtagccgtgg tgcagccggc gagcctgccc tccgcgggcg
     2461 cggtcacggc gttctacaac acctatcggt cggccccgat gtggttccgc ggcggcgcgg
     2521 cgaacccggc cgccggcaag ctcgtctcga tcctcaggcg cgcccagttc gacggccttg
     2581 cgtccgggcc gcagctcgcc tcccaggtcg aggccgcgat cacccgcgcc gcgagcggca
     2641 gcccctccga cgtcatggcg gccgagcaga ttctgtccgc ggcctgggtg atgtacgtcc
     2701 aggcgctcaa gcgtccgacg ccgcggatga tctacgccta tccggtgctc gcgccccaga
     2761 acacgcggcc cgaccagatc ctgttgaccg cggccgcggc gccgtcgctc gaggcgcacg
     2821 tgaatgcggt gtccagcatc aacccgacct atgcccagat ccgcgaagcg gcgtggctgc
     2881 aggcccaggc gtccggaacc gggccggacc cgcgcctgct cgccaacctc gagcgggcac
     2941 ggccattgcc ggccggcggc cgctacatcg tcgtcgattc cgccacccaa cggctgacga
     3001 tgtacgagaa cggccagccg gtggattcga tgaaggtcgt cgtcggcacc cgcgacctgc
     3061 cgacgccgct gatcgccagc atgatccact atgtgacctt caatccctac tggaacgtgc
     3121 ctcaccatct ggtccgcaag accgtcgccc ccaacgtgct caagcagggg ctcggctatc
     3181 tcaagagccg cggctacgag gtgatggcgg actggagcga ggaggcggcg gtcctgccgg
     3241 cggactcggt cgactggaaa gccgtcgccg ccggcaagac ccagatccgc gtccgccaga
     3301 agccggggcc cggcaattcg atgggcgaga tgaagttccg ctttccgagc ggcgaggaca
     3361 tctacctgca cgacaccccg acccgggagt atttcgccaa atcccagcgg acgctcagca
     3421 atggctgcgt ccggctcgag gatgccaagc gcctcgcccg gtggctgatg ggccgcgagc
     3481 ccctcgctcc gtccaaggac ccggagatcc gagtccagat cccgcagggg gtgccgatct
     3541 atttgaccta tctgaccgcc cagccgagca gcagcggagt tacctatctg tcggacgtct
     3601 acgggtggga tcagccggcc ggccagcagg tggcttccag caaatagcct cgccccagga
     3661 cccgcgcctc tccggcgggt tccttgtgct ggcttgccct gtcgaacggc gccttcgcgc
     3721 gcgcgtcccc caaaagaaaa ccggcccgga ggcgaacccc cgggccggca tttcctgcgt
     3781 cagcctggct gacgccgtcg cttacccgcg ctccggctcc ggcggcggcg gcggcggcgg
     3841 cggcggcgga accgggcaca cgtcggtcgc caggatcacc gaaccgtccg ggcacgtctg
     3901 cgtcgccgga ggcggcggag gcggcggagg cggaggcggc ggaggcggcg gcggcggcgg
     3961 cggcgcggcg aagttgtaga tcaggctcgc cagcaggctg tgggagcgcc atttgccgat
     4021 gagatcaact ggcgtaactc cgccaactac gccagaaccg gcatcctcgt cgtggaactt
     4081 gatccggccc gtgttgaaga aacgatactt caagccgagg tcgatgttgg ggctcagggc
     4141 cgtgcgaacc ccggcgatga cctgccaggc aagcgtgctg tcggtgtcgc ggaagctgat
     4201 tccaacctcg tccgcatcgg cgcgatactt gactcgggcc agtccgaagc cgggaccgac
     4261 atagccgctc cagccgtcgt catcgccgaa atcgagaagg aggttgacca ttgccgaaat
     4321 ggcggtggcg cggccgtcgg catcgaagtt atcgcccggg ccctcgtcgg aaatgaacgg
     4381 gctgagctcg agctcgtcaa cgctggcgcg cttgtaggca agctcgccct cgacgcggac
     4441 cattccgaaa tcgtaaccga cgatcccgtc gatgtcgaag ccggtcttgt gcttgacgat
     4501 gacggcatct tcgatctcgc tcggctggaa agggaatagt ccctcatccg aataatcgag
     4561 gttgagatcc tcgacgagca tcagtccgcc ttcgataccg gcgtagagcg aaccgtcgcg
     4621 agcgacggcc ggcgtggcca acatcgtcga tgcgagcgcc atgccaatgg ccagcttgcg
     4681 cataattgtc ccctttcagc gtgcgttctg gagttgaact gcccgaacac attatgagag
     4741 cgccggtttc cgcgcaaggt cggtcttcgc gccagctgtt gcccaaaagc ctcaactatc
     4801 ggctgtcttc gcactaattc gccggttcag cgccggtttc ctagcttgcg atcagcccgt
     4861 gctcgcgcat cgcggcgagg atctcgccga tcgccgccct ggcttcggtg tcgaccatgg
     4921 tgccgcccgc cggggacgcg atcgtcgctc cgcgcgcccc gaccactttc tggccgtcga
     4981 cgatgacttc ggaaccgcgc agcttgccga tttcccacga tccgttgcgg tagaccgcca
     5041 tgccgccgct ggtgtaggcg gcgagctggt tggcgtgccc ggcccagtcg ccgctcgggc
     5101 tgctcccgac gatatagctg ctcccgacga tatagccgct cccgaccgcc ggcgacgccg
     5161 gcggggccgc ctgcgggagc ccttcgacgg cggccgcgac caccgcgtcg agcacctgca
     5221 gcgcctcgtt gtgaaacaaa tccttctgcg cctgcccggc catttgtcac tattcctgtc
     5281 gtgtaccgaa tgagcttggc gctgtcaagc gctttttacc agttaggtta taaaaacctg
     5341 gccgcggcgg ttcagacggg gtgcagcgcg ccgcggtaat cggggtcctc gagggtccgc
     5401 ccggcgccga gcgcgacgca gctcatcggg tcctcggcga cccgcaccgg cagtcccgtc
     5461 tcgtcggcga gcacctcctc gatgccgcgc agcagcgacc cgccgccggt cagagtgatc
     5521 ccctcgtcga tgatgtcggc ggcgatttcc ggctgggtgt gctcgagggc gacccggacg
     5581 atgtggacga tccgggcgac cggctcggcg agcgcgtcgg cgatctcggc ctggttgagg
     5641 ctgatctcgc tcggcacgcc cttggcgacg tcgcggccgc ggacataggc gaccagcccc
     5701 ttgccggact cgggcggctt ggcggtgccg atttccttct tgacccgctc ggcggtcgct
     5761 tcgccgatca gcagattgta gttgcgccgc acatacgagc tgatcgcctc gtccatcttg
     5821 tcgccgccga tccgcgccga ctggctgtag gcgagcccgc gcagcgagat caccccgacc
     5881 tcggtggtgc cgccgccgat gtcgacgacc atcgagccga ccggatcggt gaccgacagg
     5941 ccggcgccga ttgcggcggc catcggctcg tcgatcagcg acacctggct cgccccggcg
     6001 tgcgacgccg cgtcgcggat cgcccgccgc tcgaccgacg tcgagccgct cggaacgcac
     6061 agcacgatct cgggccggcg cgacaggcgg cttcgcccgc cctgcgcctt ctcgacgaaa
     6121 tgcttgatca tctgctcggc gacctcgagg tcggcgatca ctccgttgcg cagcgggcgg
     6181 atggtcttga tgtagtccgg ggtcttgccc atcatcagct tggcgtcggc tccgaccgcc
     6241 ttcacccgcc ggaccccgtt cagcacctcc atcgccacca ccgagggctc gctgatgacg
     6301 atcccgcgcc cctgcacgta gaccaccgtg ttggcggtgc cgaggtcgat cgcgatcgcg
     6361 cgcgaggcga ggcggaacag gcgcggcagc ggcataaggc gaaagacctc ctcgtcggcc
     6421 cagccgggtc ccggcggccg ccggcgcatt ctaggcccac ccccgccgac tcgcgaaacg
     6481 atccgtcgtc cgtggaccat gggccgttgc gtcgccgcgc cgaacggaac ggccaccgcc
     6541 aaggacacca gcggacgcgc atatcgttgt tgcccgcttg agcgaaccgc ctggccccaa
     6601 aagccctgta cccccaacct gccgcacgag ggcctgctcg agctgctcgc cggtcagctc
     6661 cggtaaatcc gccgggcttg atgaggccgg ggattttcgc gcatggaggc aagatgacgc
     6721 tcccccgtac cctcttcagg attgtcgccc cgctggccct gctggcatcg gccgaaggcg
     6781 ccgtcgcggc gaccctgctg gtcaccaaga ccgccttttg cggctgctgc cggatctggg
     6841 tcgagcggat gaaggcggcc ggtttcacgg tcaaggtcaa cgacgtcgag aacgtcaccc
     6901 cgaccgcgcg ccggctggga gttcccgaca aattgcgctc gtgccacacc gccgagatcg
     6961 gcggctatgc gatcgagggc cacgtccccg ccgccgacat caagcggctg ctcgcgcaga
     7021 agcccaaggc ggcggggatc gcggttcccg gcatggtcgc gggcactccg gggatggagc
     7081 agggcggcca ctcggagccg taccaagtga tcctgttcga ccgcgccggc aagacgaagg
     7141 tcttcgcttc ccactgatcg cggattgagc gagccgggcc ggctcgcggc cccgcacagc
     7201 ctatttcagg atggcgctcg ccgccgtcac cacccgccac tgggtcgcgg cggtgcgctg
     7261 ctgctgcagc ggccggtagg acggcgccgc ggcgccggtg ccgaacgcga aatggctgtg
     7321 gtcgccctcg tcgagcgatt cgatcagccg gtagccggcg ttgcggaacg cggccgcgat
     7381 ctgcgcgtgg ctgaccccgg gccggcgggc gatgtcgatc gcccggccgc tcagatggaa
     7441 gctgttgcgg accccgccga ctcggcggtt gtgctcgcgc gagcgccagg tgctcgtcac
     7501 gcggccccat tgggagccga ggctggccgc cgccgtcagg ctgatccgcc cgcccggcac
     7561 ggccgccgcc gttgccaccg acgcggccgc cgccggctcg ctggccagcg ccgcctcgcc
     7621 cgacaccgcc cggaccccga agtcggtcgg ctgcgaggcc gcccacgcgg gcgccgcgcc
     7681 cgccgccaga gccagacctg ccaagacttt cagcatgcga aatccccccg ggccgcaaag
     7741 ctttgtatac acattgttaa ccgcccggac aaccgcccgg acgcccgatt ggcagcccgt
     7801 ttcgggacgg gtttccacgc tcgccgggcg ggctataacg gcgccagctc gccacgcgcc
     7861 ggccctccgc ggagaaagct ttcagtgccc agacgcgtca aggacttcat cgagatcagc
     7921 gaatacacct cgctcgacga cctgatccgc ttcctcgaga cgatccgcgc cacgcttccc
     7981 cccgattccg aggccgagct caagatccgc ggcgacgagg tgttcggccg ccggctgacc
     8041 atcaccttcc ttcgcggcct gaccgacgag gaagcctcgc tcgaggcgcg ctacaccggc
     8101 gacgtcccgc ccgagcccga ctcggcgatc gagcagctgc gcgccaagct cgacaaggtc
     8161 ccataccgcg actcgggccg ctagccgcga ttttccggaa cccagccgat cagcagcatc
     8221 tgggcggcga acccgaccac ataagcgatc ggcacgaacc aggcttcctt cagccaccgc
     8281 cacgccgacc gcgcctccgg gtacatcacc gtcaccgcca ctcccgccga cgacccgaac
     8341 cacatcatcg agccgccgaa cccgaccgca taagccagca atgcccagtc gtagccgccc
     8401 tgctttaatg ccagcatggt cagcgggatg ttgtcgaaca ccgccgacag gaaacccagc
     8461 gcggcgaccg actcccagcc cgggctcggc aggctgtcga ccggcatcag cgacgccgcc
     8521 gcgacgagca gcaccaggaa cagcgcgccc tgcgcggccg gcttggccac cgaccactcc
     8581 ggcgggccgg ccgggctggt ggcgaggatc gcgccccaga tcgccaggcc gagccacggg
     8641 gcggcctcgc cgaccccggt cgcgttcgcc cagatgttcg ccgcgaccgc ggccgccagg
     8701 atgaccccga cgatcgtgat ccgccgccac tgcagcgggt ggtcgggctc gttgtgggcg
     8761 aggatcggct ggtggcggtg ctgggcccag gagccggcga ccgcgaacac cgcgaacgcc
     8821 gggatcgccg ccgcataggc cggaagcacc gcaatcggcg acactccatg cagccacatc
     8881 agcgtggtcg tggtgtcgcc gatgacgctg cccgctccgc cggcattggc ggcggcgacg
     8941 atcgcggcga ggaagccgac cccgacgcgc cccttgtaga cgtggcgggc gatcaccgcg
     9001 ccgagcaccg ccccggcgat attgtcgagg aacatcgaca tcacgaacac catggcaagc
     9061 agcaccagcc cgccggtcca gccgtcggga agcagccgcg gcagatggtc ggggacgttg
     9121 ctctgctcga actggttgga caggatctcg aacccgacca gcagcagcag caggttggtg
     9181 agggtcaccc actcatgggc gagatgctcg ccgagcgctc cgagccccgg gccggtcgga
     9241 aactgggtga ccagcccctg gtaggcgagg atcgccacca ggcccgccgc ggcgatcggc
     9301 agcacatggc ggtggaacgc cgcgatcgcc gcgagcatca gcccgaacag cacgaactcg
     9361 acgggcactc cgaacaattc tggaaaagcg gcgaccgtcg acacactctc gttcacgcgt
     9421 tctcacccct ggttgcgccg cctgccggcg cgtcccagtg gccttgccag ccaattttcg
     9481 gcagtgcaac gagccgccga tgcccgcacc ggaatccagc tcccgcttcg tcatcgccaa
     9541 gtccgcgacg cacggatgcc gctgagctcc atccgcaacc cggcccggct ggtgccgatc
     9601 gcctttttcg tcgcgatcct gatcggcacc gcgctgctga tgattccggc cgcccgcgcc
     9661 gggcccggcg gggcgccgct gctgaccgct ttgttcaccg ccacctcggc ggtgtgcgt
//
LOCUS       DASTZO010000318         4331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_829786,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000318 DASTZO010000000
VERSION     DASTZO010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_829786"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2096)
                     /gene="katG"
                     /locus_tag="VFU20_09015"
     CDS             complement(<1..2096)
                     /gene="katG"
                     /locus_tag="VFU20_09015"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011542431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HEU5286628.1"
                     /translation="MDAKTGESMGCPVDRPTSVRSLLGRTNRDWWPESLPLEILNQGG
                     LSPDPMGDDFDYAAAFEALDYQALKDDLKALMTDSQPWWPADYGHYGPLFIRMAWHAA
                     GTYRTADGRGGANSGQQRFAPLNSWPDNGNLDKARRLLWPVKQKYGQNISWADLFILA
                     GNVAIESMGGPIFGFGGGRKDVFEPERDIYWGAEEKWVNEGSETRIQPDREMELEHPL
                     AAIQMGLIYVNPEGPGGVPDPLQSARDIKITFERMAMNHEETVALTAGGHTFGKAHGA
                     GDPSLVDAAPEAADIALQGLGWASAHGSGFGEHTITSGIEGSWVNTPTQWSENYFRLL
                     LDYEYELVTSPAGAKQWRPIDQREEDMAPAAHDPSKRVPTMMTTADMALKLDPEFRVI
                     SEKFRKDHEAFKDAFARAWFKLTHRDMGPKARYLGPEVPDEDLIWQDPVPAGKQPSDA
                     DVAAFKTKVLGAGFTVGELVKTAWASASTYRRSDHRGGANGARIRLEPQRNWAVNEPE
                     ELGRILAKIDELRGSLSMADAIVLAGTAAVEKAAQDAGFKVEVPFTGGRGDATQDWTD
                     ADSFAVMEPAADGFRNYLKTKHSVKTEELLVDRASLLGLSVPEMTVLLGGLRVLGANH
                     KDRPEGVFTNRKGQLTNDFFVNLLDNDSFWEVIDTSADEEFIGYDRGGNQEKWRATRT
                     DLIFGSNSQLRAVAEV"
     gene            complement(2246..2815)
                     /gene="pgsA"
                     /locus_tag="VFU20_09020"
     CDS             complement(2246..2815)
                     /gene="pgsA"
                     /locus_tag="VFU20_09020"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU5286629.1"
                     /translation="MLSLPNLLTLSRIFAVPILVFLLWRPTPIDYAITFVLYCIVGVT
                     DYFDGYLARAQGRISRLGQFLDPIADKIMVAAVIVMLLASRRANGEPPIIQDLHVIPA
                     LIILLREIIVSGLREFLAEVRIGVPVSALAKWKTTLQLVALGALILGGAVPAQPWVHD
                     VGLASLWAAAALTLVTGYDYLRTGLRHMD"
     gene            complement(2871..3434)
                     /locus_tag="VFU20_09025"
     CDS             complement(2871..3434)
                     /locus_tag="VFU20_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286630.1"
                     /translation="MAREARKGGGRSWSPWRIAGWSIPALLLLLPAVAMQFTSEVDWT
                     ASDFVFAGMLFGSVGLAFELIVRKSRSLAYRFGAGLAVVAAFLTVWVNGAVGMIGSED
                     NPYNLVFGGVLVVALVGAVLARFEASGMGRAMAVTAVAQAAAGAVGFATDARGAALSM
                     AFAGQWLLSAASFRNAAREQAAANGAN"
     gene            3514..>4331
                     /locus_tag="VFU20_09030"
     CDS             3514..>4331
                     /locus_tag="VFU20_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU5286631.1"
                     /translation="MLKNDAPGMTLGSFRFHRFTLDPGNRRLTLDGEPVELNARYLDA
                     LALLVREAGRLVSKDRFLDEVWRGVPVTDEALTQCIKTLRGRLGDDAASPRFIATVPK
                     HGYRFIAPVESAGDGPDSPGPAPPARESAGDSAAAHGWRRFFLLGGAGTIGAGVAGLV
                     GGLFYGFAGASQPLAPGMGAASVLLVLMWLTIAAALMGGAGVSFGIAAAGFASGRSTA
                     WAIAGGALGGLLVGGAVKLLGLDAFNLLLGQSPGDITGAAEGALLGGRLMGGSL"
BASE COUNT          634 a         1499 c         1497 g          701 t
ORIGIN      
        1 tagacttcgg caaccgcgcg gagctggctg ttcgacccga agatgaggtc ggtgcgcgtc
       61 gcgcgccatt tctcctggtt gccgccgcgg tcgtagccga tgaactcctc gtcggcgctc
      121 gtgtcgatca cttcccagaa gctatcgttg tcgaggaggt ttacgaagaa gtcgttggtc
      181 agctggccct tgcggttggt gaacacaccc tccggccggt ccttgtgatt ggcgccgagc
      241 acgcgaagcc cgcccagcag caccgtcatt tccggcacgg acaggccgag cagcgaggca
      301 cggtcgacca gcagttcctc ggtcttcacc gaatgcttcg tcttcagata gttgcgaaag
      361 ccgtcggccg ccggctccat caccgcgaag ctgtcggcat cggtccagtc ctgggtcgcg
      421 tcgccgcggc cgccggtgaa cgggacttcg accttgaagc ccgcgtcctg ggccgccttc
      481 tcgaccgccg cggtgccggc gagcacgatc gcgtcggcca tcgacagcga tccgcgcagc
      541 tcgtcgatct tcgcgagaat tcggccaagc tcctcgggct cgttgaccgc ccagttgcgc
      601 tgcggctcca gccggatgcg cgcgccgttg gcgccgccgc ggtggtccga ccggcggtag
      661 gtggaggccg acgcccaggc ggtcttgacc agctcgccga cggtgaagcc cgcgccgagg
      721 accttggtct tgaacgccgc gacgtccgcg tcggaaggct gctttccggc gggcaccggg
      781 tcctgccaga tcagatcctc gtccggcact tccggtccga ggtagcgggc cttggggccc
      841 atgtcgcggt gggtgagctt gaaccaggcg cgggcgaacg cgtccttgaa cgcctcgtgg
      901 tccttgcgga acttctccga gatcacccgg aactcggggt cgagcttgag cgccatgtcc
      961 gccgtcgtca tcatcgtcgg gacgcgcttc gacgggtcgt gggccgccgg ggccatgtcc
     1021 tcctcgcgct ggtcgatcgg ccgccactgc ttggcgcccg ccgggctggt gacgagctcg
     1081 tactcatagt cgagcagcag gcgaaaataa ttctccgacc attgggtcgg cgtgttgacc
     1141 cacgagcctt cgatgccgct ggtgatcgtg tgctcgccaa agccgctgcc gtgcgcgctc
     1201 gcccagccca ggccctgcaa ggcgatgtcg gcggcttcgg gcgcggcgtc gaccaggctc
     1261 gggtcgccgg cgccgtgcgc cttgccgaac gtgtgcccgc cggcggtgag cgcgaccgtc
     1321 tcctcgtggt tcatggccat gcgctcgaag gtgatcttga tgtcgcgcgc cgactgcagc
     1381 gggtcgggga ccccgcccgg gccttcgggg ttgacgtaga tgaggcccat ctggatcgcc
     1441 gccagcgggt gctcgagctc catctcgcgg tcgggctgga tgcgggtttc cgagccctcg
     1501 ttgacccatt tctcctcggc gccccaatag atgtcgcgct cgggctcgaa cacgtccttg
     1561 cggccgccgc cgaagccgaa gatcggcccg cccatcgatt cgatcgcgac gttgccggcg
     1621 aggatgaaca ggtcggccca gctgatgttc tggccgtatt tctgcttgac cggccacagc
     1681 aggcgccggg ccttgtcgag gttgccgttg tccggccagc tgttcaacgg cgcgaaccgc
     1741 tgctgcccgc tgttggcgcc gccgcggccg tcggcggtgc ggtaggtgcc cgcggcatgc
     1801 caggccatgc ggatgaacag cggcccgtaa tgcccgtaat cggccggcca ccacggctgg
     1861 ctgtcggtca tcagcgcttt caaatcgtcc ttgagcgcct ggtagtcgag cgcctcgaac
     1921 gccgcggcat aatcgaaatc gtcgcccatc gggtcgggcg aaaggccgcc ctggttgaga
     1981 atctccagcg gcagggattc gggccaccag tcgcggttgg tgcggccgag cagcgagcgc
     2041 acactggtcg ggcgatctac cgggcagccc atcgattcgc cggtctttgc gtccatgatc
     2101 ctcgcctctc cgtttggatg tccgagcctg ccctaggccg ggcgcggcat cgagggaaac
     2161 gattaaactt ggtcaggacg acgcgcaaaa tcgattagtc cgcgcgcttc cagtgctcgc
     2221 catgcccccg gcatggctgc gcgcttcagt ccatgtgcct gaggccggtg cgcagatagt
     2281 cgtagccggt gaccagggtg agggccgcgg ccgcccacag gctggcgagg ccgacgtcgt
     2341 ggacccacgg ctgggcgggg accgccccgc cgaggatcag cgcgcccaat gcgaccagct
     2401 gcagcgtcgt cttccatttg gcgagcgcgc tcaccggcac cccgatccgc acctcggcga
     2461 ggaattcgcg caggcccgag acgatgatct cgcgcaagag gatgatcagc gccgggatca
     2521 cgtgcaggtc ctggatgatc ggcggctcgc cgttggcccg ccgcgaggcg agcagcatga
     2581 cgatcaccgc ggcgaccatg atcttgtcgg cgatcgggtc gaggaactgg ccgagcctcg
     2641 agatccgccc ctgcgcgcgg gccagatagc cgtcgaaata atcggtgacg ccgacgatgc
     2701 agtagaggac gaaggtgatc gcatagtcga tcggcgtcgg ccgccacagc aggaacacca
     2761 ggatcgggac cgcgaagatc cgcgacaggg tcagcaggtt cggcaggctc agcatcgccg
     2821 ccgcctctac gcgaaaccgc ggcagccgca aaagccgcga ttgccgggcc ctaattcgcg
     2881 ccgttcgccg ccgcctgctc gcgagccgca ttccggaacg aagcggccga cagcagccac
     2941 tggccggcga aggccatgct gagcgccgcc ccacgggcat cggtggcgaa gccgaccgcg
     3001 ccggccgccg cctgcgcgac cgcggtcacc gccatcgccc gccccattcc ggacgcctcg
     3061 aaccgggcga gtaccgcccc gaccagcgcc acgaccagca ctccgccgaa gaccagattg
     3121 tagggattgt cctccgagcc gatcatcccg accgcgccgt tgacccacac ggtgagaaaa
     3181 gcggcgacga ccgcgagccc ggcgccgaag cgataggcga ggctgcgcga cttgcggacg
     3241 atgagctcga aggccaggcc gacgctcccg aacagcattc cggcgaagac gaaatcggac
     3301 gcggtccagt cgacctcgct cgtgaactgc atcgcgaccg cgggcagcag caggaggagc
     3361 gcggggatgc tccagccggc gatccgccac gggctccagc tgcgcccacc gcccttgcgg
     3421 gcctcccttg ccatttgact tgcctccacc gggtgaatca ggctttcaac ctggcacggg
     3481 cgccggtgaa gggcatgagc aacttgtgag gttatgctga aaaatgacgc gccggggatg
     3541 acattgggca gctttcgctt ccaccgcttc acgctcgacc ccggcaaccg ccggctgacg
     3601 ctcgacggcg agccggtcga gctcaatgcc cgctatctcg atgccctcgc tctgctggtg
     3661 cgcgaagccg gcaggctggt ctccaaggac cgcttcctcg acgaggtgtg gcgcggagtg
     3721 ccggtcactg acgaggcgct cacccagtgc atcaagacgc tccgcggccg gctcggcgac
     3781 gacgccgcca gcccgcgctt catcgccacg gtccccaagc acggctaccg cttcatcgcg
     3841 cccgtggaat cggccggcga cgggccggac agcccgggcc cggcgccgcc cgctcgcgag
     3901 tccgcgggcg attccgcggc cgctcatggc tggcggcggt ttttcctgct cggcggggcc
     3961 gggacgatcg gggccggggt cgccgggctc gtcggcgggc tgttctacgg ctttgccggg
     4021 gcgtcgcagc cgctggcgcc cggcatgggc gcggcctcgg tgctgctggt gctgatgtgg
     4081 ctgaccatcg ccgcggccct gatgggcggc gccggagtca gcttcggaat cgccgcggcg
     4141 ggcttcgcct cggggcgctc gacggcgtgg gccattgccg gcggcgctct cggcgggctg
     4201 ctggtgggcg gcgccgtcaa gctgctcggg ctcgacgcgt tcaacctgct gctcggccag
     4261 tcgccgggcg acatcaccgg agcggccgag ggggcgctgc tcggcggacg gctgatgggg
     4321 ggcagcctcg a
//
LOCUS       DASTZO010000319         3213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_856974,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000319 DASTZO010000000
VERSION     DASTZO010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_856974"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..132)
                     /locus_tag="VFU20_09035"
     tRNA            complement(57..132)
                     /locus_tag="VFU20_09035"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(97..99),aa:Lys,seq:ctt)
     gene            complement(244..1356)
                     /gene="rodA"
                     /locus_tag="VFU20_09040"
     CDS             complement(244..1356)
                     /gene="rodA"
                     /locus_tag="VFU20_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010126421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HEU5286632.1"
                     /translation="MISSAIIPQPLARLPWRLIFLVAGIAGIGLLTLYSAAGGSIRPW
                     ALRQGTTFLFFLCVAVAMSWVKVSTIKSLAFPVYGAVLVMLILVEMLGFVTKGAQRWL
                     DVGFIRLQPSEFMKPAIVLVLARFYELLPVGYIRKWRAIWPAGLLIGVPAALILVQPD
                     LGTCIMVVLGGLTVMFVAGVPMRLFLGGAAALAVAAPIAFSMMHEYQRKRVFTFLDPE
                     SDPLGAGYHISQSKIAIGSGGIFGKGFLNGSQSHLDYLPEGHTDFAFAAMVEEWGLLG
                     GVLLIVAFGAVIRWGMTVSLRSASRFAQLAAAGLSATIFFYVAINLMMVMGLAPVVGI
                     PLPLVSFGGSAVMTVMLCLGLLMALERQQRTTSKLA"
     gene            complement(1353..>3213)
                     /gene="mrdA"
                     /locus_tag="VFU20_09045"
     CDS             complement(1353..>3213)
                     /gene="mrdA"
                     /locus_tag="VFU20_09045"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017670839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEU5286633.1"
                     /translation="LLAGGAQAAVGAILIGRLGWLSVAENERYQLMSESNRVQLIIVP
                     PRRGWIIDRHGKPIAINRSDFRVDLIPEQLGDVEQTLTTLTELLDLNVDDVDRILKEL
                     KAARGYQPVQVAENVPYENYAAVTVRLPELPGVQPMRGFSRYYPDGPAVGHLVGYVGA
                     ASAKEYEKEKNPLLITPGFKIGKEGLEETLEKNLRGTPGGQRVELTARGKLVRELTPK
                     PDRSGATVPLAIDSDLQEFAARRLGEESGSVVVLDCLTGDVLALVSMPAYDPNSFSDG
                     IGRTEWKMLSEDPRRPLLNKVLNALYPPGSTIKPMGALALLQHGVEPQERIFCPGGYR
                     LGNRFFRCLGRHGSVDMRRAIAKSCNTYFYAMGHRIGYDRIAPMAKRLGLGQKFDLPV
                     VSQSYGTVPDSEWKERRYKQNKRLIERPDWTASDTLNASIGQGFLIINPMQLAVMGGR
                     IASGRDIQPRLIGVGKKPAPLLGLPVEHLEIVRGGMWEVVNGDGTAGASRLPLPGIEM
                     SGKTGTAQVRRIAGGQRGQSGEWKYRDHGLFVCFAPSANPRYAASVVIEHGLGGARAA
                     APVAKDVLTFLFDRAKALESLAALEQQWGGTLAERTARRAAAIKAASDARAGA"
BASE COUNT          525 a         1115 c         1082 g          491 t
ORIGIN      
        1 tgactacttt agctggcacc gtctgcggcg ccattcttct aagtctttga aaagaatggt
       61 ggacgcacta gggctcgaac ctaggacccg ctgattaaga gtcagctgct ctaccaactg
      121 agctatgcgt ccaatgccgt caggcagtcg cgccggcggc cgtccgctcg ttgcgcggcg
      181 gcgccggtag catgggccaa ggtggggtgc aacgcctggc attcaaccgg cgaaggtccg
      241 gggtcaggcg agcttcgacg tcgtccgctg ctgccgctcg agcgccatca gcagccccag
      301 gcacagcatc accgtcatca ccgccgagcc gccgaagctg accagcggaa gcgggatccc
      361 gaccaccggg gcgagcccca tgaccatcat caggttgatc gcgacataga agaagatcgt
      421 cgccgacagg ccggcggcgg cgagctgggc gaagcggctc gccgagcgca ggctgaccgt
      481 catcccccag cggatcacgg ccccgaacgc gacgatcagc agcactccgc cgagtagccc
      541 ccattcctcg accatcgccg cgaaggcgaa atcggtgtgg ccttcgggca gatagtcgag
      601 gtggctctgg ctgccgttca ggaagccctt gccgaagatg ccgcccgagc cgatcgcgat
      661 ctttgactgg ctgatgtgat agccggcgcc cagcggatcg ctttccgggt cgaggaaggt
      721 gaacacgcgc ttgcgctgat attcgtgcat catgctgaag gcgatcggcg ccgcgaccgc
      781 cagcgccgcc gctccgccca ggaacaggcg catcggcacg cccgcgacga acatcaccgt
      841 cagcccgccg agaacgacca tgatgcaggt gccgagatcg ggctggacca ggatcagcgc
      901 ggcgggaacg ccgatcagca gtcccgccgg ccagatcgcc cgccacttgc ggatgtagcc
      961 gaccggaagc agttcgtaga agcgcgcgag caccagcacg atcgccggct tcatgaactc
     1021 gctcggctgg aggcggatga agccgacgtc cagccagcgc tgcgcgccct tggtgacgaa
     1081 gccgagcatc tcgaccagga tcagcatcac cagcacggcc ccgtagaccg ggaaggcgag
     1141 gctcttgatg gtcgagacct tcacccacga catcgccacc gcgacgcaca ggaagaacag
     1201 gaaggtcgtg ccctgccgga gtgcccatgg gcggatcgag ccgccggccg ccgaatagag
     1261 cgtgagaagg ccgatgccgg cgatgcccgc gaccaggaag atcaaccgcc acggcagcct
     1321 ggccagcggc tgggggatga tcgccgacga gatcatgcgc cggcccgggc gtcggacgcc
     1381 gccttgatcg ccgcggcccg gcgggcggtc cgctcggcca gcgtgccgcc ccattgctgc
     1441 tcgagcgcgg ccagcgattc cagcgccttg gcccggtcga acaggaaggt cagaacatcc
     1501 ttggccaccg gcgcagcggc gcgggcgccg ccgaggccgt gctcgatcac caccgaggcc
     1561 gcgtagcgcg ggttggcgga cggggcgaag cagacgaaca ggccgtggtc gcggtacttc
     1621 cactcgccgc tctggccgcg ctggccgccg gcgatccgcc gcacctgcgc ggtgccggtc
     1681 ttgccgctca tctcgatgcc gggcagcggg agcctgctgg cgcccgcggt gccgtcgccg
     1741 ttgaccacct cccacatgcc gccgcggacg atctcgagat gctcgaccgg caggccaagc
     1801 agcggcgccg gtttcttgcc gaccccgatc aggcgcggct ggatgtcgcg gcccgacgcg
     1861 atccgtccgc ccatcacggc cagctgcatc gggttgatga tcaggaagcc ctggccgatc
     1921 gacgcgttga gggtgtccga cgcggtccaa tccggccgct cgatcagccg tttattctgc
     1981 ttgtagcgcc gctccttcca ctcgctgtcg gggacggtgc cgtagctctg gctgaccacc
     2041 ggcaggtcga acttctggcc gagcccgagc cgcttggcca tcggcgcgat cctgtcgtag
     2101 ccgatgcggt ggcccatcgc gtagaaatag gtgttgcagc tcttggcgat cgcccggcgc
     2161 atgtcgacgc tgccgtggcg gccgaggcag cggaagaagc ggttgccgag ccggtagccg
     2221 cccgggcaga agattcgctc ctgcggctcg actccatgct ggagcagggc cagcgcgccc
     2281 atcggcttga tcgtcgagcc cggcgggtag agcgcgttca gcaccttgtt gagcagcggc
     2341 cggcgcgggt cctccgacag catcttccac tcggtgcggc cgatgccgtc cgaaaagctg
     2401 ttggggtcgt aggccggcat cgacaccagc gccaggacgt cgccggtcag gcaatcgagc
     2461 accaccaccg agcccgattc ctcgccgagc cgccgggcgg cgaactcctg caggtcggaa
     2521 tcgatcgcca gcggcaccgt cgccccgctg cggtcgggct tgggggtcag ctcgcggacc
     2581 agcttgccgc gcgcagtcaa ttcgacccgc tggccgccgg gagtgccgcg caaattcttc
     2641 tcgagcgtct cctcgagccc ctccttgccg atcttgaagc ccggggtgat cagcagcggg
     2701 ttcttctctt tctcatattc cttggccgac gcggcgccga catagccgac cagatggccg
     2761 accgccggcc cgtccgggta atagcgcgag aagccgcgca tcggctggac cccgggaagc
     2821 tcgggcagcc gcaccgtcac cgccgcgtaa ttctcgtagg ggacgttctc ggccacctgg
     2881 accggctggt agccgcgcgc cgccttgagc tccttcaata tccggtcgac atcgtcgacg
     2941 ttgaggtcga gcagctcggt cagggtggtc agcgtctgct cgacgtcgcc gagctgttcc
     3001 gggatcaggt cgacccggaa gtcgctccgg ttgatggcga tcggcttgcc gtggcggtcg
     3061 atgatccagc cgcgccgcgg cggcacgatg atcagctgca cccggttgct ttcggacatc
     3121 agctggtagc gctcgttctc ggcgaccgac agccagccga gccggccgat caggatcgcc
     3181 ccgaccgccg cctgggcgcc gccggcgagc agc
//
LOCUS       DASTZO010000320         9476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_731089,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000320 DASTZO010000000
VERSION     DASTZO010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9476
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_731089"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..602)
                     /locus_tag="VFU20_09050"
     CDS             complement(<1..602)
                     /locus_tag="VFU20_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5286634.1"
                     /translation="MSYDPVIDGEIAAVVTYLEMLAPRPAPVPDSPLSLRRIERPDPE
                     RYRRLFRLVGARWLWFSRLVMADSELAAILGDPGVELYAVVDGAGNEAGLLELDFRQG
                     GQCELAFLGLVPELAGQGHGRWLLAEALRLAWRPDVTRVHVHTCTLDHPAALGAYRRA
                     GFAAYKREVERFPDPRLSGVLPPDCAPQLPLVGTLASAAPS"
     gene            complement(730..1584)
                     /gene="hemF"
                     /locus_tag="VFU20_09055"
     CDS             complement(730..1584)
                     /gene="hemF"
                     /locus_tag="VFU20_09055"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HEU5286635.1"
                     /translation="MKPLDEQQQAARDWFEALRTRICAEFEAIEREAGSDATFDYTPW
                     ERTDTGGGPGAKDGGGGVRGHMAGRVFEKVGVNVSTVGGRFSEEFAATIPGAEEDPSF
                     FATGLSLVAHMANPHVPAVHMNTRFITTTRRWFGGGADLNQAIPYPEDTDAFHARLRA
                     ACAAHDPTFYPRFSKWADEYFWIAHRAVPRGVGGIFYDHLEGHFDAHFAFTRDVGEAF
                     LDIFPKIVRKRMDTPFTDAEMEALLEFRGRYVEFNLLYDRGTLFGLKTGGNIDAILMS
                     LPPLAKWV"
     gene            1756..2334
                     /gene="petA"
                     /locus_tag="VFU20_09060"
     CDS             1756..2334
                     /gene="petA"
                     /locus_tag="VFU20_09060"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013041172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HEU5286636.1"
                     /translation="MATLEQSAEGSEIAHADGGEGVRRRDFINVAALSFAGVGVVAAS
                     LPLITQMNPSADVLALASTEVDISKIAPGQAIKTIWRKQPVFVRNLTPQEIAAANKVP
                     LGDLRDPQTLAERTKPGKPNWLITLGVCTHLGCVPLGTGEGENRGEYGGYFCPCHGSH
                     YDTAARIRKGPAPLNLQVPEYQFLSDTVVQIG"
     gene            2351..3646
                     /locus_tag="VFU20_09065"
     CDS             2351..3646
                     /locus_tag="VFU20_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010217844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6"
                     /protein_id="HEU5286637.1"
                     /translation="MSFSWARPYEPKSPFMRWLDERLPLPRLVYGAVGGGYPVPRNLN
                     YWWNYGVLAGVFLTIQIVTGIVLAMHYYASADGAFNSVEHIMRNVNAGWLLRYAHANG
                     ASFFFIVTYIHIFRGLFYGSYKAPRELVWMLGLVIYLLMMATAFMGYVLPWGQMSYWG
                     AQVITGFFSAFPLVGEPVRIWLLGGYAPDQAALTRFFSLHYLLPFIIAAVVVLHIWAL
                     HIPGSGNPTGVDVKDEKDTLPFHPFYTAKDGWFAAGFLALFALVLYFAPNALGHPDNY
                     IPANPLATPAHIVPEWYFWPFYVILRAFTVDFILPAKLWGVLAMFASILLLFFLPWLD
                     KSPVRSGHYRPVFKRFFWILLLDIFVLGLCGMKPAVQPWVAIGQLATAYYFLHFLVIL
                     PLIAAFETPLPLPDSISSSVLHGEKKEAAPLGAAPAAAE"
     gene            3658..4587
                     /locus_tag="VFU20_09070"
     CDS             3658..4587
                     /locus_tag="VFU20_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015457150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HEU5286638.1"
                     /translation="MLLRFIMGSIGVGFALVLLVSLVTDLSGYFGSPPEKSAEEEFHK
                     HPKHLALASDGPFGKFDRQQLQRGFQVYKEVCAACHGLSLVAFRDLHDLGYEEPEVKA
                     IANQWAIEVPTENPETGEPATRKAIPSDNFPSPYANETAARAANNNALPPDLSLIAKA
                     REGGAAYTYSLLTGYQRQPARLLKEFPAAKTPEGLHYNPYFANLNIAMPPPLVSDGQV
                     TYAPGNPAPTVDQMARDVSAFLVWAAEPKLERRHRSGIAVVIFLLVGTVFGYLAYRQI
                     WAEAKRKVRPTGPLDPKNIAKSRRAKSRQGVTG"
     gene            4688..5302
                     /locus_tag="VFU20_09075"
     CDS             4688..5302
                     /locus_tag="VFU20_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010128011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286639.1"
                     /translation="MRILNLRTAAIVLAAGAGLGGCATYSPFGYGSGVSVSYGDRGYY
                     DPYYGGYGYGHGYSPYGYRSAYGYSPYWGWNNGYYYPGTGYYVYDRYRRPRVWTDAER
                     QYWTERANSPGTTTTRRLLDNWADFSNGGQTTQSTQTTRSVRESDSSFGDRRAARRAQ
                     RIERATRPTAEREQSTRIERRIERARSSGRVRVVGGERSETQPE"
     gene            complement(5321..5776)
                     /locus_tag="VFU20_09080"
     CDS             complement(5321..5776)
                     /locus_tag="VFU20_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HEU5286640.1"
                     /translation="MKYFEDIAAGDETLFGHYDVTRDEVLEFASRYDPQPFHLSDEAA
                     ARTHFGRLSASGWHTCAMTMAVIARYVVADEQAGLGSPGVDELRWLKPVYPGDRLTVR
                     GTVVEKVPSRSKPHIGVIRTRTTVSNQDGLDVMSFVSIVMMQRRPEGDG"
     gene            complement(5935..8382)
                     /locus_tag="VFU20_09085"
     CDS             complement(5935..8382)
                     /locus_tag="VFU20_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003050738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5286641.1"
                     /translation="MLASTVLASPGIAAAQDTGAGEPAGTAAKTEVASDSQDIIVTAQ
                     RRAENLQDVPIAITAITTKTLEDNQVDAFDDYAKMVPSLSYRSAGPGFANVYFRGVAS
                     GENANHSASLPSVGTYLDEQPITTITGALDLHVFDIARVEALAGPQGTLYGASSQAGT
                     VRLITNKPDLSGTYGAVNLEVNKVAHGDFGYTGEGFVNLPLSSKIAARVVGWYRRDGG
                     YIDNIEGQLVFPLKYCCDEDGNLIDDPGDEVTVDNSEFAEDDYNDVYTHGARAALRIE
                     LDDNWTITPQVMGQRQKSYGSFAEESGLGELETMQFHYEKTDDKWWQAALTVEGKVGN
                     FDMTYAGAYMKRQIDGEFDYSDYTYFYDALYGYAVYWYDNDTGTVVDPRQWIESDDSF
                     TKQSHELRFTSPADKPIRLVGGLFYQRHTHNIEQNYIIDNLTDTFVVTGTDSNIWLTK
                     QFRVDRDYAAFGELSYDVMPRVTLTAGGRLYRYNNSLIGFFGFNSNYSSRTGEAACFG
                     PPEVGGSPCTNLDKSTKGSGFVHRLNATYKPNDDLLLYATWSEGFRPGGINRRGELPP
                     YKPDFIDNYEAGAKFSFGRGSHFNVAVYQLNWKDIQLSFLGQNGLTEIRNAGNARIRG
                     IEADLLLRPVEGLTWSTGAAYNNAKLRNDFCLIAVENFDCTVDVDLDGDGIPEENAEL
                     APSGTRLPLTARWKANSRVRYEWPLMTDTRAHVQASLTYEGERRRDLRTGINAIYGNM
                     DSYTLVDAAAGVERGPWTASLFVKNLFDVRGELTKSIQCVETTCGDPDGLTAMGGKIY
                     TTVTRPRTIGLRIGRKF"
     gene            complement(8514..>9476)
                     /locus_tag="VFU20_09090"
     CDS             complement(8514..>9476)
                     /locus_tag="VFU20_09090"
                     /EC_number="2.8.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013848487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0050656 - 3'-phosphoadenosine
                     5'-phosphosulfate binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HEU5286642.1"
                     /translation="GKALEDRREDEQAFAHYSEGNRIRAGQLHYDPQVITAHVEACEA
                     LFSRDFLAGRQGLGCAAPDPIFILGMPRAGSTLVEQILASHPLVEGTMELPDIIALSR
                     RVGRSDDEARSEPGKGMRYPAILAELTADELRALGQDYLDRTRIQRSSGKPFFIDKMP
                     NNWTHTGFIRLILPNARIIDARRQPLACCFSNFKQHFARGQAFSYSLETLGAYYRDYV
                     RLMAHFDWVLPGVVHRVIHEELVADPEAEIRRLLDALGLPFDDSCLRFYENTRPVRTA
                     SSEQVRRPISSEGLDQWRRFEEWLGPLKAALGPALGGWDDAPKP"
BASE COUNT         1457 a         3148 c         3235 g         1636 t
ORIGIN      
        1 gagggcgccg cggaggccag cgtcccgacc agcggcagct gaggagcgca gtcgggcggc
       61 aggacgccgc tgagccgcgg gtcggggaag cgctcgacct cgcgcttgta ggcggcaaag
      121 ccggcgcggc ggtaggcgcc aagcgcggcc gggtggtcga gcgtgcaggt gtggacgtgg
      181 acgcgggtta cgtccggtcg ccacgcgagc cgcaacgcct ccgccagcag ccagcggccg
      241 tggccctggc cggcgagctc gggcaccaga cccaggaaag cgagctcgca ctgcccccct
      301 tggcgaaagt cgagctcgag caggccggcc tcgtttccgg cgccgtcgac gaccgcgtac
      361 agctcgaccc cgggatcgcc gaggatcgcg gccagctccg aatcggccat caccagccgc
      421 gagaaccaca gccagcgtgc cccgacgagc cggaacagcc gccggtatcg ctccggatcg
      481 gggcgctcga tgcggcgcag cgacagcggc gaatcgggca ccggcgcggg gcggggggct
      541 agcatctcga ggtaagtgac gaccgccgcg atctcgccgt cgatgaccgg atcgtagctc
      601 atcggccctc cgcgggccgg ggaggtggca gcgcgcagcg ctgacggagg ggggactgag
      661 cctggcgcgg ggccccttcc accatccccg ctttcgcgag gatggtcccc ttccccgttg
      721 cggggaggat catacccatt tggccagcgg cggcaggctc atcaggatcg cgtcgatgtt
      781 gccgccggtc ttgagcccga acagagtgcc gcggtcgtag agcaggttga actcgacgta
      841 gcggccgcgg aactcgagca gggcttccat ctccgcgtcg gtgaacggcg tatccatgcg
      901 cttgcggacg atcttcggga agatgtcgag gaacgcctcg ccgacgtcgc gggtgaaggc
      961 gaaatgggcg tcgaaatgcc cctcgagatg gtcgtagaag atgccgccga cgccgcgcgg
     1021 caccgcgcgg tgggcgatcc agaaatattc gtcggcccat ttggaaaagc gcggatagaa
     1081 ggtcgggtcg tgggcggcgc aggcggcccg cagccgggcg tggaacgcgt cggtgtcctc
     1141 cgggtagggg atcgcctggt tgaggtcggc gccgccgccg aaccagcgcc gggtggtggt
     1201 gatgaagcgg gtgttcatgt gcaccgcggg gacgtgcggg ttggccatgt gggcgaccag
     1261 gctcaatccg gtggcgaaga agctcgggtc ctcctcggcg ccggggatgg tggccgcgaa
     1321 ctcttcgctg aaacggccgc cgacggtcga gacgttgacc ccgaccttct cgaacacccg
     1381 gccggccatg tggccgcgga cgccgccccc tccatccttg gcgcccgggc cgccgccggt
     1441 gtcggtccgc tcccacggcg tgtagtcgaa cgtcgcgtcg ctgcccgcct cgcgctcgat
     1501 cgcctcgaac tccgcgcaga tccgggtgcg aagcgcctcg aaccagtcgc gcgccgcctg
     1561 ctgctgctca tcgaggggtt tcatggcggt ggcgatgaag gcaaagcgac ggccaatcaa
     1621 gagccccgaa ttccgcccct tggcagccta ggacgcgccc gctatcaggc gcgccaatcg
     1681 cctccgggaa cgccggaggc ggcctcgctc ggtgcttgtc cggcacccgg gcaaattcgc
     1741 gtcacaaggg ttcgaatggc cacgctggaa cagtccgcag agggttccga gatcgcccac
     1801 gcagatggcg gcgagggcgt ccgccgccgc gatttcatca acgtcgcggc gttgagcttc
     1861 gccggtgtcg gagtggtcgc ggcgagcctt ccgctgatca cccagatgaa cccgtcggcg
     1921 gatgtcctgg cgctggcttc cacggaagtg gatatctcca agatcgcccc cgggcaggcg
     1981 atcaagacca tctggcgcaa gcagccggtg ttcgtccgca acctgacccc gcaggagatc
     2041 gcggcggcca acaaggtgcc gctgggcgat ctgcgcgatc cgcagacgct cgccgagcgg
     2101 accaagccgg gcaagcccaa ctggctgatc acgctgggcg tctgcaccca tctgggctgc
     2161 gtcccgctgg gcaccggcga gggggagaac cgcggcgagt acggcgggta tttctgcccg
     2221 tgccacgggt cgcactacga caccgccgcg cgcatccgca aaggcccggc gccgctcaac
     2281 ctgcaggtgc ccgaatacca gttcctgtcc gacaccgtgg tccagatcgg ctgaggggga
     2341 tcaccgccag atgagcttct cctgggcgag gccctacgaa cccaaatcgc cgttcatgcg
     2401 ctggctcgac gagcggctgc cgctgccgcg cctcgtctac ggggcggtcg gcggcggcta
     2461 cccggtgccg cgcaacctca actattggtg gaattacggc gtcctcgcgg gcgtcttcct
     2521 gaccatccag atcgtcaccg gcatcgtcct ggcgatgcat tattacgcgt cggccgacgg
     2581 cgccttcaat tcggtcgagc acatcatgcg caacgtcaac gccggctggc tgctgcgcta
     2641 cgcccacgcc aacggcgcga gcttcttctt catcgtcacc tacatccaca ttttccgcgg
     2701 cctgttctac ggcagctaca aggcgccgcg cgagctggtg tggatgctcg gcctggtcat
     2761 ctatctgctg atgatggcga ccgccttcat gggctacgtg ctgccgtggg ggcagatgag
     2821 ctattggggc gcgcaggtca tcaccggctt cttctcggcc ttcccgctgg tcggcgagcc
     2881 ggtgcggatc tggctgctgg gcggctacgc tcccgaccag gcggcgctga cccgcttctt
     2941 ctcgctccac tatctgctgc cgttcatcat cgccgcggtg gtcgtgctcc acatctgggc
     3001 gctgcacatc ccgggctcgg gcaacccgac cggggtcgac gtcaaggacg agaaggacac
     3061 gctcccgttc cacccgttct acaccgccaa ggacggctgg ttcgcggccg gcttcctcgc
     3121 gctgttcgcg ctggtgctct atttcgctcc caacgcgctc ggccaccccg acaattacat
     3181 tccggccaac ccgctggcga ctccggcgca catcgtcccc gaatggtatt tctggccgtt
     3241 ttacgtgatc ctcagggcgt tcaccgtcga cttcatcctg ccggccaagc tgtggggcgt
     3301 gctggcgatg ttcgcctcga tcctgctctt gttcttcctg ccgtggctcg acaagtcgcc
     3361 ggtccgctcg gggcattacc ggccggtgtt caagcgcttc ttctggatcc tgctgctcga
     3421 catcttcgtc ctgggcctgt gcgggatgaa gccagcggtc cagccgtggg tggcgatcgg
     3481 ccagctggcg accgcctatt acttccttca cttcctggtc atcctgccgc tgatcgcggc
     3541 gttcgagacg ccgctgccgc tgcccgattc gatctcgagc tcggtgctcc acggggaaaa
     3601 gaaggaagct gctccgctgg gcgccgcgcc ggcggcggct gaatagggaa acggtcgatg
     3661 ctcctgcgct tcatcatggg ttcgatcgga gtcggtttcg cgctggtcct gctcgtttcg
     3721 ctggtgacgg acctgtccgg ctacttcgga tcgccgcccg aaaagagcgc cgaggaagag
     3781 ttccacaagc atcccaagca ccttgcgctg gccagcgacg ggccgttcgg caagttcgac
     3841 cgccagcagc tgcagcgcgg gttccaggtc tacaaggagg tctgcgcggc ctgccacggc
     3901 ctctccctgg tcgcgttccg ggacctgcac gacctgggct acgaggagcc cgaggtgaag
     3961 gcgatcgcca accagtgggc gatcgaggtg ccgaccgaga accccgagac cggcgagccg
     4021 gccacgcgca aggcgatccc gtccgacaat ttcccgagcc cgtacgcgaa cgagaccgcg
     4081 gctcgggcgg ccaacaacaa tgcgcttccg cccgacctgt cgctgatcgc caaggcgcgg
     4141 gaagggggcg ccgcctacac ctactcgctg ctgaccggct accagcgcca gccggccagg
     4201 ctgctcaagg aattcccggc ggcgaaaacg cccgagggcc ttcactacaa cccctatttc
     4261 gccaacctca acatcgccat gccgccgccg ctggtcagcg acggccaggt cacctacgcg
     4321 cccggcaacc cggcgccgac ggtcgaccag atggccaggg acgtgtcggc cttcctcgtc
     4381 tgggcggccg agcccaagct cgagcggcgc caccgctcgg gaatcgcggt ggtgatcttc
     4441 ctgctggtcg gcaccgtctt cggctatctc gcctaccggc agatctgggc cgaggcgaag
     4501 cgcaaggtcc ggccgaccgg gccgctcgac ccgaagaata tcgccaagag ccgccgggcc
     4561 aagtccaggc agggcgtaac cggttgatta ccggcggttt ccggcgcgtc tagagcgggt
     4621 taattcagcc ccggttcact cgccggggac gaaatcgagc gcaactcctc tctcgcggag
     4681 atctgttatg cgtatcctca atcttcgtac cgccgcgatc gtgctggccg ccggcgccgg
     4741 ccttggcggc tgcgccacct acagcccgtt cggctacggc agcggcgtgt cggtcagcta
     4801 cggcgaccgc ggctattacg atccctatta cggcggctat ggctacggcc acggctattc
     4861 gccctacggc taccgctccg cctacggcta ttcgccttac tggggctgga acaacggcta
     4921 ttattacccg ggcaccggct attacgtgta cgaccggtac cgtcgcccgc gcgtgtggac
     4981 cgacgccgag cggcaatatt ggaccgagcg cgccaacagc ccgggcacga ccacgacccg
     5041 caggctgctc gacaattggg ccgatttctc gaacggcggg cagacgaccc agagcaccca
     5101 gaccacccgg tcggttcgcg aaagcgattc gagcttcggc gaccgccggg ccgcgcggcg
     5161 agctcagcgc atcgagcggg cgacccggcc gacggccgag cgtgagcagt cgacgcgcat
     5221 cgagcgccgc atcgaacggg cgcgctcgtc cggccgagtc cgggtcgtcg gcggcgagcg
     5281 gtccgagacc cagcccgagt aagcgctagc cggcgcccgt ctagccatcg ccctctgggc
     5341 ggcgctgcat catgacgatc gagacgaagc tcatcacgtc gagcccgtcc tgattgctga
     5401 cggtggtgcg cgtccggatg accccgatgt gcggcttgga gcgcgacggc actttctcga
     5461 cgaccgtccc gcggaccgtc agccggtcgc cgggatagac cggcttcagc cagcgcagct
     5521 cgtcgacccc cggcgaaccg aggcccgcct gctcgtccgc gacgacgtag cgggcgatca
     5581 ccgccatggt catcgcgcag gtgtgccagc cgctcgccga cagccgcccg aaatgggtcc
     5641 gggccgcggc ctcgtcggac aggtggaacg gctgcgggtc gtatcggctc gcgaactcga
     5701 gcacttcgtc gcgggtgacg tcgtaatggc cgaacagagt ctcgtcgccg gccgcgatgt
     5761 cctcgaaata tttcattcgt gggggccgta caatttcctc ccctcgattt cgaggggagg
     5821 ggaaccgtcc ccgcgcaggc ggggatggtg gaggggccgt ttccgcgcag agcccctcca
     5881 ccatcgaatc tgcgttcgat ggtccccctc cccggcaaat gccggggagg aattttagaa
     5941 cttgcgtccg atgcgcagtc cgatggtgcg cggccgggtc acggtcgtgt agatcttgcc
     6001 gcccatcgcc gtcagcccgt ccggatcgcc gcaggtcgtc tcgacgcact ggatgctctt
     6061 ggtgagctcg ccgcgcacgt cgaacaggtt cttgacgaac aggctcgcgg tccacggccc
     6121 gcgctcgacc ccggcggcgg cgtcgaccag cgtgtaggag tccatgttgc cgtagatggc
     6181 gttgatgccg gtgcgcaggt cgcgccggcg ctcgccctcg taggtcaggc tggcctggac
     6241 gtgggcgcgc gtgtcggtca tcagcggcca ttcgtagcgc actcggctgt tcgccttcca
     6301 gcgcgcggtc agcggcaggc gcgtgcccga aggcgcgagc tccgcatttt cctcgggaat
     6361 gccgtcgccg tcgagatcga cgtccaccgt gcagtcgaaa ttctccaccg cgatcaggca
     6421 gaagtcgttg cgcagcttgg cgttgttgta ggccgcgccg gtgctccacg tgagcccttc
     6481 gaccggccgc agcagcaggt cggcctcgat gccgcgaatg cgcgcattgc ccgcgttgcg
     6541 gatctcggtg aggccgttct ggccgaggaa ggacaattgg atgtccttcc agttcagctg
     6601 atagacggcg acgttgaagt gcgacccgcg gccgaagctg aacttcgcgc ccgcctcata
     6661 attgtcgatg aagtcgggct tgtagggcgg cagctcgccg cgccggttga ttccgcccgg
     6721 gcggaagccc tccgaccagg tggcgtagag cagcaggtcg tcgttgggct tgtaggtcgc
     6781 gttcaggcgg tggacgaagc cgctgccctt ggtgctcttg tcgaggttgg tgcaggggga
     6841 gccgccgacc tcgggcggcc cgaagcaggc cgcttcgccg gtccggctgc tgtagttcga
     6901 gttgaacccg aagaagccga tcagcgaatt gttgtaccgg tagagccggc cgccggccgt
     6961 cagggtcacc cggggcatca cgtcgtacga tagctcgccg aacgccgcat agtcgcggtc
     7021 gacccggaat tgcttggtca gccagatgtt gctgtcggtt ccggtgacga cgaacgtgtc
     7081 ggtcagattg tcgatgatgt agttctgctc gatgttgtgc gtgtggcgct ggtagaacag
     7141 gccgccgacc agacggatcg gcttgtcggc cggcgaggtg aagcgcaatt cgtgcgactg
     7201 cttggtgaag ctgtcgtcgg actcgatcca ctgcctcggg tcgacgaccg tgccggtgtc
     7261 gttgtcgtac cagtagaccg catagccgta gagcgcgtcg tagaaatagg tgtagtccga
     7321 atagtcgaac tcgccgtcga tctggcgctt catgtaggcg ccggcatagg tcatgtcgaa
     7381 attgccgacc ttgccctcga cggtcagcgc cgcctgccac catttgtcgt cggtcttctc
     7441 atagtggaac tgcatcgtct cgagctcgcc caggccgctc tcctcggcga acgagccgta
     7501 gctcttctgc cgctggccca tgacctgcgg agtgatggtc caattgtcgt cgagctcgat
     7561 ccgcagcgcg gctcgggcgc cgtgggtgta gacgtcgttg taatcgtcct cggcgaactc
     7621 ggaattgtcg accgtgacct cgtcgcccgg gtcgtcgatc agattgccgt cttcgtcgca
     7681 gcaatatttg agcgggaaga ccaattgccc ctcgatattg tcgatgtacc cgccgtcgcg
     7741 ccggtaccag ccgacgaccc gcgccgcgat tttgctcgag aggggcaggt tgacgaagcc
     7801 ttcgccggtg tagccgaaat cgccgtgggc gaccttgttc acctcgaggt tcaccgcgcc
     7861 gtaggtgccg ctcagatccg gcttgttggt gatcagccgg accgtgccgg cctggctgga
     7921 ggcgccgtac aaagtgccct gcgggccggc gagcgcctcg acccgggcga tgtcgaagac
     7981 gtggaggtcg agcgccccgg tgatggtcgt gatcggctgc tcgtcgagat aggtgccgac
     8041 gctcggcagc gaggccgagt ggttggcgtt ctcgccgctg gcgacgccgc ggaaatagac
     8101 gttggcgaac cccggcccgg ccgagcggta cgacaaggac ggcaccatct tcgcatagtc
     8161 gtcgaacgcg tcgacctggt tgtcctcgag ggtcttggtg gtgatcgcgg tgatcgcgat
     8221 cggcacgtcc tgcaaattct cggcgcggcg ctgggcggtg acgatgatgt cctggctgtc
     8281 gctggcgacc tcggtcttgg cggcggttcc cgccggctcg cccgcgccgg tgtcctgggc
     8341 cgccgcgatc cccggcgagg ccagcacggt agaagcaagc aagatagccg ccgagcggcg
     8401 ggtccaatga gccgattgca tcgtcgttcc ctcttgtttc gcacctgcaa cactgtgcct
     8461 gcgcggattg ggcgtcaatt gctttgtcgc cgcggggtga aaaaatggcg cgcttatggt
     8521 tttggcgcat catcccagcc gccgagcgcc gggccgagcg ccgccttgag cgggccgagc
     8581 cactcctcga accgccgcca ctggtcgaga ccctcgctgc tgatcggccg gcgcacctgc
     8641 tccgagctgg cggtccggac cgggcgggta ttttcgtaga agcgcaggca ggagtcatcg
     8701 aacggcagac cgagcgcgtc gagcagccgg cggatttcgg cctcggggtc ggcgaccagc
     8761 tcctcgtgga tcacgcggtg gacgacgccc ggcaggaccc agtcgaagtg cgccatcagc
     8821 cgcacatagt cgcggtaata ggcgccgagc gtctccaggg agtaggaaaa ggcctggccg
     8881 cgcgcgaaat gctgcttgaa gttggaaaag cagcaggcga gcggctggcg gcgggcgtcg
     8941 atgatccggg cgttgggcag gatcagccgg atgaagccgg tgtgggtcca gttgttgggc
     9001 atcttgtcga tgaagaaggg cttgccgctc gagcgctgga tccgcgtgcg gtcgagatag
     9061 tcctggccga gcgcccggag ctcgtcggcg gtgagctcgg cgaggatcgc gggatagcgc
     9121 atgcccttgc ccggctcgct ccgggcctcg tcgtcgcttc ggccgacgcg ccgcgacaga
     9181 gcgatgatgt cgggcagctc catcgtccct tcgaccagcg ggtggcttgc gaggatctgc
     9241 tcgaccaggg tcgagccggc gcgcggcatt ccgaggatga agatcgggtc gggagcggcg
     9301 catccgagcc cctggcgccc ggcgaggaag tcgcggctga acagcgcctc gcacgcctcg
     9361 acgtgggcgg tgatcacctg cgggtcgtaa tgaagctggc cggcgcggat ccggttgcct
     9421 tccgaataat gggcgaacgc ctgctcgtcc tcgcggcggt cttcgagcgc cttgcc
//
LOCUS       DASTZO010000321         4641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_103828,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000321 DASTZO010000000
VERSION     DASTZO010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4641
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103828"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFU20_09095"
     CDS             <1..544
                     /locus_tag="VFU20_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016745459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HEU5286643.1"
                     /translation="FYAPKLEWHLTDAIGRTWQCGTLQLDPHMPQRLDATYIGADGDK
                     HFPLMLHRAILGTFERFIGILIEHHAGKFPLWLAPVQAVVATIVSDADDYAGEVAAKL
                     ASAEIRVATDLRNEKINYKVREHSLAKVPLLLVVGKREADERSVALRRLGSEDRQQML
                     GLDEAAAMIAAESAPPDLRP"
     gene            680..1225
                     /gene="infC"
                     /locus_tag="VFU20_09100"
     CDS             680..1225
                     /gene="infC"
                     /locus_tag="VFU20_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017184052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU5286644.1"
                     /translation="MPPPYPRRPQAPPSFSGPRYNEFIVAPKVRVIDENGENLGVMYT
                     REAIEQAAEVGLDLVEISPNADPPVAKFLDIGRYKYEAQKKANEQRKKQKTQEIKEIK
                     MRPNIDDHDYDTKMKKVFEFLEDGDKVKVTMRFRGREMAHGELGMAVLRRVQDQTAEL
                     AKVEAHPRMEGRQMLMVLAPK"
     gene            1393..4365
                     /locus_tag="VFU20_09105"
     CDS             1393..4365
                     /locus_tag="VFU20_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015459084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5286645.1"
                     /translation="MARKSLWLLSAAFFAVSVPAQAQETDTDQGSAQPTEGATAEAAA
                     VDDQAVEPQSADTSDIIVTATRRNEALSDVPLAVSAVTAETLEYSGASDIRQLTQLSP
                     SLLVSSTSSEAGAGVARIRGIGTVGDNPGLESSVGVFIDGVYRSRTGVGLTELGPVDR
                     IEVLRGPQGTLFGRNTSAGLISIITAKPRFQPSVYGQVDIGNYDLRRVELGGTGPVSD
                     TIAVRLDTVYMKREGFLEDVISGREINDRDRWLLRGQALFQPSDDLSIRLIADYAKRD
                     EECCGAPFLPARDFTAAGEQPSTIAAIERGLGAIISDDTFERETSITPGRSYRSDVKD
                     YGLSGELVYDFGGAELTSITAYRFNRYVRGQDADFNNLDILFRDDDGSAFNRFKTFTQ
                     ELRLQGEAFGDRLDWLVGGYYADEKLRVEDNLAYGADYARYANCLVANNFAAFAPTIV
                     NTASPTCFNTAVAGGLLPFLPPSQQPVLAAFARLGVFAAPIFNNSGFSNLATAFAFPG
                     QTLNLVGLDDTWNQSSRNWALFTHNIFAVTDTIKLTVGARYTNERKKLTANLRDNNTL
                     CTFFAGGALAALQQLPCVNPSVPGGVFADRDTKSESKLSGTAVLSWKPTDSLLTYLSY
                     SRGYKAGGFNLDRAALWRSNPTAPPPGADPVGGSGAVCLTATQFACQGRAASISDDLT
                     FKPETNDAFELGAKYNGRGVDVNVAVFQQEFKNFQLNTFNGLNFVVENINSCSDDLGD
                     ADEDNSPFTGECTGKLKPGVRSRGVEVEVFTRPMTDLALNLGGTLVQTKYRDDLVGAG
                     GRPLTNALFQLPNRRVSNSAEFAMTGSAAWTPPIGGGMKGLVYADFRHQSRINTGSDL
                     DLEKIQKGFTVVNGRIGLHGRDQAWGVELWAQNLFDKDYRQVAFDAPIQGSGTERAVE
                     RGFIPRATQLFGAFLAEPRTYGITLRAKFGPSRAPAPAYVEPPAPPPPPIVEEPPPPP
                     PPPPPPPPPPPPSTGQRG"
     gene            complement(4439..>4641)
                     /locus_tag="VFU20_09110"
     CDS             complement(4439..>4641)
                     /locus_tag="VFU20_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010123853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286646.1"
                     /translation="LAALARAIHDCGHRIHSAHIATYGERAVDVFYLTRADGRKLDAG
                     DAEKLRSALLAAAGDGAEAKAA"
BASE COUNT          807 a         1651 c         1491 g          692 t
ORIGIN      
        1 gttctacgcg cccaagctcg agtggcacct gaccgacgcg atcggccgca cctggcaatg
       61 cgggaccctg cagctcgacc cgcacatgcc gcagcggctg gacgcgacct acatcggcgc
      121 cgacggcgac aagcattttc cgctgatgct gcaccgggcg atcctcggca ccttcgagcg
      181 cttcatcggc atcctgatcg agcatcacgc cggcaaattc ccgctgtggc tggcaccggt
      241 ccaggcggtg gtcgcgacga tcgtgtccga cgcggatgac tatgcggggg aggtggcggc
      301 gaaactcgct tcggcggaaa tccgcgtcgc caccgacctc aggaacgaga agatcaatta
      361 caaggtgcgc gagcacagcc tcgccaaggt cccgctgctg ctggtcgtcg gcaagcgcga
      421 ggccgacgag cgctcggtcg cgctgcgccg cctgggctcc gaggaccgcc agcagatgct
      481 gggcctcgac gaggccgcgg cgatgatcgc cgccgaatcc gcgccgcccg acctgcgccc
      541 ttgagctcgc cgcacatagc gctgacgccg caccgccaca ggttggtgca aaaacgactc
      601 gggttgcggc ccggcacctt cccaactgca gtccaaccgc ctaactccgg tcggtcgcac
      661 acgtaacgga gaccagacta tacccccgcc ctatccgcgc cggccgcagg cgccgccgag
      721 cttttccggc ccccgctaca acgagttcat cgtcgccccg aaagtccggg tgatcgacga
      781 aaacggcgag aatctgggcg tcatgtacac ccgcgaagcg atcgagcagg cggccgaggt
      841 cggcctcgac ctggtcgaga tctctcccaa cgccgacccg ccggtggcca agttcctcga
      901 tatcggccgc tacaagtacg aggcccagaa aaaggccaac gagcagcgca agaagcagaa
      961 gacgcaggag atcaaggaga tcaagatgcg tcccaacatc gacgaccacg actatgacac
     1021 gaagatgaag aaggtcttcg agttcctcga ggacggcgac aaggtcaagg tgacgatgcg
     1081 cttccgaggc cgcgagatgg cgcacggcga actgggcatg gcggtgctcc gccgagtcca
     1141 ggaccagacc gccgaactgg ccaaggtcga ggcccatccg cgcatggaag ggcgccagat
     1201 gctgatggtg ctggctccga agtaggcgca gcgcgccgct gccttccgtt acggaaagca
     1261 gcaaaaccgc ggacggttgt tgcaattgtg cagcaacgcg gcggcaatat ttttccacgc
     1321 cgccgccgtt cgcttccttt gcccgtgcga ctggcttagc ttcgccgcgc atcgctctgg
     1381 ggagaggata ggatggcgcg caaatcattg tggctgcttt cggcggcctt tttcgctgtt
     1441 tcggttccgg cccaggcgca ggaaaccgac accgaccagg gatcggcgca gccgaccgag
     1501 ggcgcgaccg ccgaagccgc cgcggtcgac gaccaggcgg tcgagccgca gtcggccgac
     1561 accagcgaca tcatcgtcac cgcgactcgg cgcaacgagg ccttgtccga cgtcccgctc
     1621 gcggtcagcg cggtgaccgc cgagaccctc gaatattcgg gcgccagcga cattcgccag
     1681 ctgacccagc tgtcgccctc gctgctggtc tcgtcgacct cgtccgaagc cggcgccgga
     1741 gtcgcgcgaa tccgcggcat cggaacggtc ggcgacaatc cgggactcga aagttcggtc
     1801 ggcgtgttca tcgacggagt ctatcgctcg cgcaccggag tcgggctgac cgagctcggg
     1861 ccggtcgacc gcatcgaggt cctgcgcggg ccgcagggca ccctgttcgg ccgcaacacc
     1921 tcggcgggcc tgatctcgat catcaccgcc aagccgcgct tccagccgtc ggtctacggc
     1981 caggtcgaca tcggcaatta cgacctgcgc cgggtcgagc ttggcggcac cgggccggtg
     2041 tccgacacca tcgcggtgcg cctcgacacg gtctacatga agcgcgaagg cttcctcgag
     2101 gacgtgatct cgggccgcga gatcaacgac cgcgaccgct ggctgctgcg cgggcaggcg
     2161 ctgttccagc ccagcgacga cctgtcgatc cggctgatcg ccgattacgc caagcgcgac
     2221 gaggaatgct gcggggcgcc gttcctcccg gcgcgcgact tcaccgcggc gggcgagcag
     2281 ccctcgacca tcgccgcgat cgagcgcggg ctcggagcga tcatcagcga cgacacgttc
     2341 gagcgcgaaa cgtcgatcac cccgggccgt tcgtaccggt ccgacgtcaa ggactacggc
     2401 ctgtcgggcg agctggtcta cgatttcggc ggcgccgagc tgacctcgat caccgcctac
     2461 cggttcaacc gctacgtgcg cgggcaggac gccgacttca acaatctcga catattgttc
     2521 cgcgacgacg acggcagcgc gttcaaccgc ttcaagacct tcacccagga gcttcggctg
     2581 cagggcgagg cgttcggcga ccggctcgac tggctggtcg gcggctatta cgccgacgag
     2641 aaactgcggg tcgaggacaa cctcgcctac ggcgcggatt atgcgcgcta cgcgaactgc
     2701 ctggtcgcga acaatttcgc ggcgttcgcc ccgaccatcg tcaacacggc gtcgccgacc
     2761 tgcttcaaca cggcggtggc gggcgggctg ctgccgttcc tgccgccatc ccagcagccg
     2821 gtcctcgccg ccttcgcccg gctcggcgtg ttcgcggcgc cgattttcaa caacagcggc
     2881 ttttcgaacc tcgcgaccgc cttcgccttt cccggccaga cgctcaacct ggtcggtctc
     2941 gacgacacgt ggaaccagag cagccgcaac tgggccttgt tcacccacaa catctttgcg
     3001 gtcaccgaca cgatcaagct gacggtcggc gcccgctaca ccaacgaacg caagaagctg
     3061 accgcaaacc tgcgcgacaa caatacgctc tgcaccttct tcgcgggcgg cgcgctcgcg
     3121 gccttgcagc agctgccgtg cgtcaacccc agcgttcccg gcggagtctt tgccgaccgc
     3181 gacaccaaat cggaatccaa gctgtcggga accgccgtgc tgagctggaa gccgaccgac
     3241 agcctgctca cttatttgag ctattcgcgc ggctacaagg ccggcgggtt caacctcgac
     3301 cgagctgcgc tgtggcgcag caacccgacg gcgccgccgc cgggggcgga cccggtcgga
     3361 ggcagcggcg cggtgtgcct gaccgccacg caattcgcct gccagggccg ggcggcgtcg
     3421 atcagcgacg atctgacctt caagccggaa accaacgacg ccttcgagct cggcgccaaa
     3481 tacaacggcc gcggcgtcga cgtgaacgtc gcggtgttcc agcaggagtt caagaacttc
     3541 cagctcaaca cgttcaacgg cctcaacttc gtcgtcgaga acatcaacag ctgctccgac
     3601 gaccttggcg acgccgacga ggacaacagc ccgttcaccg gcgagtgcac cggcaagctg
     3661 aagcccggcg tccgcagccg cggagtcgag gtcgaggtct tcacccggcc gatgaccgac
     3721 ctggcgctga acctcggcgg gaccctcgtc cagaccaagt atcgcgacga cctcgtcggc
     3781 gccggcgggc gcccgctgac caacgccctg ttccagcttc cgaaccggcg ggtttcgaac
     3841 agcgccgagt tcgcgatgac cggctcggcc gcctggacgc cgccgatcgg cggcgggatg
     3901 aagggcctgg tctatgcgga cttccgccac cagtcgcgga tcaacaccgg ctccgacctc
     3961 gatctcgaga agatccagaa gggcttcacc gtcgtcaacg ggcgcatcgg cctgcatggt
     4021 cgcgaccagg cctggggcgt cgagctgtgg gcgcaaaatc tgttcgacaa ggactatagg
     4081 caggtggcgt tcgacgcgcc gatccagggc tcgggcaccg agcgcgcggt cgagcgcggc
     4141 ttcatcccgc gcgcgacgca attgttcggc gcgttcctcg ccgagccgcg cacctacggc
     4201 atcaccctgc gcgccaagtt cggcccgagc cgagcgccgg ctccggccta tgtcgagccg
     4261 ccggcgccgc cgccgccgcc gattgtcgag gagccgccgc cgccgccgcc gccgccacct
     4321 ccgccgccgc caccgccgcc gccttcgacc ggccagcgcg gctgatcggt tcaaccgggg
     4381 agcaagggga gggccctgcc ggaaacggcg gggcccgctt cttttccagc ccgctttcct
     4441 aggccgcctt cgcctccgcg ccgtcgccgg cggcggcgag cagcgccgag cgcagcttct
     4501 cggcgtcgcc ggcatcgagc ttccggccgt ccgcccgggt caggtagaag acgtcgacgg
     4561 cgcgctcgcc gtaagtggcg atgtgggccg aatggatccg gtgcccgcaa tcgtggatcg
     4621 cccgcgccag cgcggcgagc a
//
LOCUS       DASTZO010000322         7596 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_570801,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000322 DASTZO010000000
VERSION     DASTZO010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_570801"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1412)
                     /gene="hutH"
                     /locus_tag="VFU20_09115"
     CDS             complement(<1..1412)
                     /gene="hutH"
                     /locus_tag="VFU20_09115"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004212473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEU5286647.1"
                     /translation="MKLNPEAIDLATLRKLWRGEPASLDSAALQRVTDAAASVERIVA
                     SGETVYGVNTGFGSLANTRIPDGRLAELQRNLILSHSAGLGEPLPRHVTRLMIVLKLL
                     GLGRGHSGVRSLIIAALQGLLDTDSMPVIPSQGSVGASGDLAPLAHLIAALMGHGSID
                     VAGEILPAAEALRKLGLEPLELAPKEGLALINGTQASAAIALDALFMGERVFAAALAA
                     GALSVDALKGSAKPFDPRVSALRGQPGQVRVAAEIAALLDGSEILASHGRCGKVQDPY
                     SFRCQPQVMGAALDLLMNAARTLTIEAGAVTDNPIVFPDEDSAISGGNFHAQPVAFAA
                     DTIALALCEVGSISERRTAVLVDPKMSGLPPFLTEDSGVNSGMMIPQVTAAALVAENR
                     SLAFPASVDSIPTSAGQEDHVSMAPISALKAGRIARNAAGIVAVELIAAAQGVDCHAP
                     LTTSARLQAIHASVRALSPHL"
     gene            complement(1409..3085)
                     /gene="hutU"
                     /locus_tag="VFU20_09120"
     CDS             complement(1409..3085)
                     /gene="hutU"
                     /locus_tag="VFU20_09120"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEU5286648.1"
                     /translation="MSANIDEGRRDNRRHIRARRGSEIVARSWATEAAVRMLMNNLDE
                     EVAEDPQSLVVYGGIGRAARNWQCFDAIVATLERLDDDQTLLVQSGKPVGVFRTHRDA
                     PRVLIANSNLVPKWASWEVFNRLDRAGLMMYGQMTAGSWIYIGTQGIVQGTFETFAEM
                     GRQHYGGDLKGKWILTAGLGGMGGAQPLAAVMAGAHCIAVEVQESRIAKRLETRYLDH
                     RAATVDEALAIIAAATEPTSVGLLGNAAELLPQMLERGIRPDALTDQTSAHDPANGYC
                     PAGWSVAQWQEMRERDPEAVAAAARQSIARHVEAMLAYQAQGIPTFDYGNNIRQEAKD
                     AGVAHAFDFPGFVPAYVRPLFCRGIGPFRWVALSGDPEDIFKTDQRVKELIPDDPHLH
                     RWLDMARTRIAFQGLPARICWVGLGQRHRLGLAFNDMVRSGEVSAPIVIGRDHLDSGS
                     VASPNRETESMKDGSDAVSDWPLLNALLNTASGATWVSLHHGGGVGMGYSQHAGMVVV
                     ADGSDDAARRLERVLWNDPASGVMRHADAGYAEAIECAKEQGLDLPMVAQ"
     gene            complement(3082..4287)
                     /gene="hutI"
                     /locus_tag="VFU20_09125"
     CDS             complement(3082..4287)
                     /gene="hutI"
                     /locus_tag="VFU20_09125"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010339115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEU5286649.1"
                     /translation="MWDRLLIDCHVATMEAVPGDPLGIVRNGSIGIQDGKIVRVGKRT
                     ELAGFRAKEVVPLDGAWVTPGLIDCHTHLVFGGNRSDEHALRRAGASYEEIAKAGGGI
                     ASTVARTRAASEPELLERALGRLRALMAGGVTTVEIKSGYGLDTDSELRLLRIARLLG
                     ETEPVRVVGTLLALHALPKGRDRDEFVAEAIEQTIPAAAKAGLATSVDAYCEAIAFTP
                     DEVARLFKAAAGHGLRVKLHAEQLSNRNGAALAAQHRALSADHLEYLDEAGARAMAEA
                     GTVAVLLPGASYALQESRKPPVPMLRDHKVRIAVATDCNPGTSPLLSPTLAINMACTL
                     FGLTPAEALAGMTINAAHALGLEKEIGSIAPGKAADLAVWRLESLAELGYWIGLPGPD
                     RRIFAGQDA"
     gene            4330..5211
                     /locus_tag="VFU20_09130"
     CDS             4330..5211
                     /locus_tag="VFU20_09130"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HEU5286650.1"
                     /translation="MGNWPNLAELLVARAGAAPVGLLGAPLAAGSVTAGACDLAPELL
                     RATLKRIGRYDVETARELATQVADRGDVDVTGLTIEAATAPLSVAVRESAEAHALTLL
                     VGGNNAVTRPAVLGLGGELEEIGLITLDAHFDMRDAAGGLSNGNPVRALLEDGLPGAN
                     IAQVGLASFANSKAMHRDAEEAGNLVVTIGDVRREGIEQAISHALDHVAHCDALVVDC
                     DVDVIDRSQFPGAPGARPGGMAVTDFFAAVRKLASDPRVRVIDLTEWDPPLDPTDLSA
                     LTAARWVAECLAGYELR"
     gene            complement(5208..5792)
                     /locus_tag="VFU20_09135"
     CDS             complement(5208..5792)
                     /locus_tag="VFU20_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008828793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HEU5286651.1"
                     /translation="MTLDLFNAPLVAGLGYRDDFIAAAEEKQLLDRLAALDLAPFRFH
                     GWLGNRKTRSFGWRYDFDDASFTPADPLPGWLLPLREKAAAFAGVAPQEFVHVLIARY
                     DPGAGIGWHRDRSVFEQVVGVSLGSPATLRFRRRKPGGFDRASLNVEPRSAYLLAGEA
                     RHQWEHSIAPGDRRRFSITFRTLSELGRRKAAQA"
     gene            complement(5789..6811)
                     /locus_tag="VFU20_09140"
     CDS             complement(5789..6811)
                     /locus_tag="VFU20_09140"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014075363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HEU5286652.1"
                     /translation="MAAPRPPAPMEALLAAELPDGPGWQYEPKWDGFRCLATRNGGGV
                     ELTSRSGKPLGRYFPEVVALLEALEEKRFLLDGELILPLGDALSFEALQMRLHPAESR
                     IRRLAQETPAEFMLFDVLSLGGRSLVGKPFAERRVALETFHAKQRVPGLLLSPATRDR
                     RTALGWLERSGGALDGVIAKRLDLDYRPGERAMVKVKQQRTADCVVGGFRYAERKHEV
                     GSLLLGLYDEQGLLHHVGFTSAIAARDRPALTDRLEALIGPLGFTGNAPGGPSRWATA
                     RSTAWEPLRPELVVEVRYDQVTGRRFRHGTGFVRWRPDKDPRQCTFDQLAPELRPSEL
                     AELFAP"
     gene            complement(6930..7006)
                     /locus_tag="VFU20_09145"
     tRNA            complement(6930..7006)
                     /locus_tag="VFU20_09145"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6970..6972),aa:Met,seq:cat)
     gene            complement(7157..7271)
                     /gene="rrf"
                     /locus_tag="VFU20_09150"
     rRNA            complement(7157..7271)
                     /gene="rrf"
                     /locus_tag="VFU20_09150"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(7386..>7596)
                     /locus_tag="VFU20_09155"
     rRNA            complement(7386..>7596)
                     /locus_tag="VFU20_09155"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1104 a         2515 c         2822 g         1155 t
ORIGIN      
        1 aggtgcgggg acagggcccg gacgctggcg tggatggcct gaagcctggc gctggtcgtc
       61 agcggggcgt ggcagtcgac gccctgggcg gcggcgatca gctcgacggc gacgattccg
      121 gccgcgttgc gggcgatccg cccggccttc agcgccgaaa tcggcgccat cgacacatgg
      181 tcctcctgcc cggcggacgt ggggatcgaa tcgacgctgg ccgggaaggc gaggctgcgg
      241 ttctccgcga ccagggccgc cgcggtgacc tgcgggatca tcatccccga attgaccccg
      301 ctgtcctcgg tcaggaacgg cggcaggccg ctcatcttcg ggtcgaccag caccgcggtg
      361 cggcgctcgg agatcgagcc gacctcgcac aaagcgagcg cgatcgtgtc ggcggcgaag
      421 gcgaccggct gggcgtggaa attgccgccg gagatcgcgc tgtcttcatc gggaaagacg
      481 atcgggttgt cggtcaccgc gccggcctcg atggtcaggg tgcgcgccgc gttcatcagc
      541 agatcgagcg cggcgcccat cacctgcggc tggcagcgga acgagtacgg gtcctggacc
      601 ttgccgcagc ggccgtggct ggcgaggatt tcgctgccgt cgagcaaggc ggcgatttcg
      661 gccgcgaccc gcacctggcc gggctggccg cgcagcgccg agacgcgcgg gtcgaacggc
      721 ttggccgagc ccttcaaggc atccaccgac agagcgccgg cggcaagcgc cgcggcgaac
      781 acgcgctcgc ccatgaacaa ggcgtcgagc gcgatggcgg ccgaagcctg ggtgccgttg
      841 atcagcgcca ggccttcctt tggcgcgagc tcgagcggct cgaggccgag cttccggagc
      901 gcctcggcgg cgggcaggat ttcgccggcg acgtcgatgg agccgtggcc catcagcgcg
      961 gcgatcagat gcgcgagcgg ggcgaggtcg cccgatgcgc cgacgctgcc ctggctcggg
     1021 atgacgggca tcgagtccgt gtcgagcaac ccctggagcg cggcgatgat cagcgagcgg
     1081 acgcccgaat ggccgcggcc gaggccgagc aatttgagga cgatcatcag ccgggtgacg
     1141 tggcgcggca gcggctcgcc gaggccggcg ctgtgcgaga ggatcaggtt gcgctgcagc
     1201 tcggcgaggc ggccgtcggg gattcgggtg ttggcgaggc tgccgaagcc ggtgttgact
     1261 ccgtagaccg tctcgcccga ggcgacgatg cgctcgaccg aggcggcggc gtcggtgacg
     1321 cgctggagcg cggcggagtc gagcgaggcg ggctcgcccc gccacagctt gcgcagggtc
     1381 gcgaggtcga tcgcttcggg gttgagcttc attgcgccac catcggcaaa tccaggccct
     1441 gttccttggc gcattcgatc gcctcggcat agccggcgtc ggcgtggcgc atgacgccgg
     1501 acgcggggtc gttccacagc acgcgctcga ggcggcgcgc cgcgtcgtca ctgccgtcgg
     1561 cgacgaccac catcccggcg tgctgcgaat agcccatgcc gaccccgccg ccgtggtgca
     1621 gcgacaccca ggtcgcgccc gacgcggtgt tgagcagggc gttcaggagc ggccagtcgg
     1681 agacggcgtc gctgccgtcc ttcatgcttt cggtctcgcg gttgggcgag gcgacggagc
     1741 cgctgtcgag atggtcgcgg ccgatgacga tcggcgccga gacctcgccg ctgcggacca
     1801 tgtcgttgaa cgcaaggccg agccggtggc gctggccgag gccgacccag cagatccgcg
     1861 ccggcagccc ctggaaggcg atccttgtcc gggccatgtc gagccagcgg tggaggtgcg
     1921 ggtcgtcggg gatcagctcc ttgacccgct ggtcggtctt gaagatgtcc tcgggatcgc
     1981 cgctcagcgc gacccagcgg aacgggccaa tgccgcggca gaacaaaggc cggacgtagg
     2041 ccgggacgaa gccggggaaa tcgaacgcgt gggcgacccc ggcgtccttc gcttcctggc
     2101 ggatgttgtt gccataatcg aaggtgggga tgccctgcgc ctggtaagcg agcatcgcct
     2161 cgacgtgcct ggcgatggac tggcgcgccg cggcggcgac cgcttcgggg tcgcgctcgc
     2221 gcatctcctg ccattgggcg acgctccagc cggccgggca atagccgttg gcggggtcgt
     2281 gggccgacgt ctggtcggtc agcgcgtcgg ggcggattcc gcgctcgagc atctgcggga
     2341 gcagctcggc ggcgttgccg agcaggccga cgctggtcgg ctcggtcgcg gcggcgatga
     2401 tggcgagcgc ctcgtcgacg gtcgcggcgc gatggtcgag gtagcgggtc tcgagccgct
     2461 tggcgatgcg gctttcctgg acctcgacgg cgatgcaatg cgccccggcc atcaccgcgg
     2521 cgagcggctg ggcgccgccc atcccgccga gcccggcggt caggatccac ttgcccttga
     2581 ggtcgccgcc gtaatgctgg cggcccatct cggcgaaggt ctcgaaggtg ccctggacga
     2641 tgccttgggt gccgatatag atccacgagc cggcggtcat ctggccgtac atcatgagtc
     2701 cggcgcgatc gagccggttg aacacctccc agctcgccca tttgggcacc aggttggaat
     2761 tggcgatcag cacccgcggg gcgtcgcggt gggttcggaa cactccgacc ggcttgcccg
     2821 actggaccag cagggtctgg tcgtcgtcga gccgctcgag cgtcgcgacg atggcgtcga
     2881 agcattgcca gttgcgcgcc gcccggccga tgccgccgta gacgaccagg ctctgcgggt
     2941 cctcggcgac ctcctcgtcg aggttgttca tcagcatccg caccgccgcc tcggtcgccc
     3001 agctcctggc gacgatctcg ctgccgcggc gggcgcggat gtgccggcgg ttgtcgcggc
     3061 ggccttcgtc aatattggcg ctcatgcgtc ttgtccggcg aaaattcgcc ggtcggggcc
     3121 cggcaggccg atccagtagc cgagctcggc gaggctttcg aggcgccaca cggcgaggtc
     3181 ggcggccttg ccgggggcga tgctgccgat ctccttttcg agcccgagcg cgtgcgcggc
     3241 gttgatggtc attccggcga gcgcttcggc gggcgtcagt ccgaacaggg tgcaggccat
     3301 gttgatcgcc aaggtcggcg agaggagagg cgaggtgccc gggttgcagt cggtggcgac
     3361 ggcgatgcgc accttgtggt cgcggagcat tggcaccggc ggcttgcggc tttcctggag
     3421 cgcgtaagag gcgccgggca ggagcaccgc gacggtgccc gcctcggcca tcgcccgggc
     3481 gccggcctcg tcgagatatt cgagatggtc ggcggacagg gcgcggtgct gggcggcgag
     3541 cgcggcgccg ttccggttgg acaattgctc ggcgtggagc ttgaccctga ggccgtggcc
     3601 ggcggcggcc ttgaacaacc gcgcgacctc gtccggagtg aaggcgatgg cctcgcaata
     3661 ggcatcgacg ctggtcgcga gccccgcctt ggcggcggcg gggatggtct gctcgatcgc
     3721 ctcggcgacg aactcgtcgc ggtcgcggcc cttgggcaat gcgtgcagcg cgagcagggt
     3781 gccgacgacg cggacgggtt cggtctcgcc gagcagcctg gcgattctca gcagccgcag
     3841 ctcggagtcg gtgtcgaggc cgtagcccga cttgatctcg acggtggtga ccccgccggc
     3901 catcagcgcg cgcagccgcc cgagcgctcg ttcgagcagc tcgggctccg aggcggcgcg
     3961 ggtcctggcg acggtcgagg cgatcccgcc gccggccttg gcgatctctt cgtacgaggc
     4021 gccggcacgg cgcagcgcat gctcgtcgga gcggttgccg ccgaacacca ggtgggtgtg
     4081 gcagtcgatg aggccgggag tcacccaggc gccgtcgagc ggcacgacct ccttggcgcg
     4141 gaagccggcg agctcggtgc gcttgccgac gcgaacgatc ttgccgtcct ggattccgat
     4201 cgagccgttg cggacgatgc cgagcgggtc gccggggacc gcttccatag tcgcgacgtg
     4261 gcagtcgatc aggaggcggt cccacatgtc ggaatcgcct tagggcagcg ccggtggtac
     4321 cgtcaaagcg tgggcaattg gccgaatctc gccgagctgc tggtggcgcg cgccggcgcc
     4381 gccccggtcg ggctgctggg ggcgccgctc gccgccggct cggtgaccgc gggcgcgtgc
     4441 gacctcgccc cggagctgct tcgcgcgacg ctcaagcgga tcggccgcta cgacgtcgag
     4501 acggcgcgcg agctcgcgac ccaggtcgcc gaccgcggcg acgtcgatgt caccgggctg
     4561 accatcgagg ccgcgacggc gccgctcagc gtcgcggtcc gggagagcgc cgaagcccat
     4621 gcgctgaccc tgctggtcgg cggcaacaac gcggtcaccc ggcccgccgt gctcgggctc
     4681 ggcggcgagc tcgaggagat cgggctgatc acgctggacg cccatttcga catgcgcgac
     4741 gcggccgggg gcctgagcaa cggcaacccg gtccgggcat tgctcgagga cgggctgccg
     4801 ggcgccaaca tcgcccaggt cgggctcgcc agcttcgcca acagcaaggc catgcaccgc
     4861 gacgccgagg aggccggcaa tctggtcgtc accatcggcg acgtccggcg cgagggcatc
     4921 gagcaggcga tcagccacgc gctcgaccat gtcgcgcatt gcgacgcgct ggtcgtcgac
     4981 tgcgacgtcg acgtcatcga ccgctcgcaa ttcccgggcg cgccgggggc ccggccgggc
     5041 gggatggctg tcaccgactt cttcgcggcg gtgcgcaagc tggcgtccga cccgcgggtg
     5101 cgggtgatcg atttgaccga atgggacccg ccgctcgacc cgaccgacct cagcgccctg
     5161 acggcggcgc gctgggtcgc cgaatgcctc gccggctacg agctgcgcta ggcctgcgcg
     5221 gccttgcgtc ggccgagctc cgacagggtg cggaaggtga tcgagaagcg ccgccggtcg
     5281 ccgggcgcga tgctgtgctc ccactggtgg cgggcttcgc cggcaagcag ataggccgag
     5341 cgcggctcga cgttgaggct cgcgcggtcg aacccgcccg gctttcggcg ccgaaagcgc
     5401 agggtcgccg gcgagccgag cgacactccg acgacttgtt cgaacaccga acggtcgcgg
     5461 tgccagccga tgccggcgcc ggggtcgtag cgggcgatca ggacgtggac gaactcttgc
     5521 ggcgcgacgc cggcaaaggc agcggctttc tcgcgcaacg gcagcagcca gccgggcagc
     5581 gggtcggcgg gcgtaaagct ggcgtcgtcg aaatcgtagc gccagccgaa gctccgggtc
     5641 ttgcggttgc cgagccagcc gtggaatcgg aacggcgcca ggtcgagcgc tgcgaggcgg
     5701 tcgagcagct gcttttcctc ggccgcggcg atgaaatcgt cgcgatagcc gaggccggcg
     5761 accagcggcg cgttgaacag gtcgagagtc atggcgcgaa cagctcggcg agctcggacg
     5821 ggcgcagctc gggcgcgagc tggtcgaagg tgcactggcg cgggtccttg tcgggccgcc
     5881 agcgcacgaa gccggtgccg tggcgaaagc ggcggccggt gacctggtcg tagcgcacct
     5941 cgacgaccag ctcgggcctc agcggctccc acgccgtcga gcgggcggtc gcccatcggc
     6001 tcggcccgcc cggcgcattg ccggtgaagc ccagcggccc gatcagcgct tcgagccggt
     6061 cggtcagcgc cgggcggtcg cgggcggcga tcgccgaggt gaagccgacg tggtggagca
     6121 ggccctgctc gtcatagagc ccgagcagca gcgagccgac ctcatgcttc cgttccgcgt
     6181 agcggaagcc gccgaccacg cagtcggcgg tgcgctgttg cttgaccttg accatcgccc
     6241 gctcgcccgg ccggtagtcg aggtcgagcc gcttggcgat gaccccgtcg agcgctccgc
     6301 cgctgcgctc caaccagccg agcgcggtcc ttcggtcgcg ggtcgcgggc gacagcagca
     6361 gcccggggac ccgctgtttc gcgtggaatg tctcgagcgc gacgcggcgc tccgcgaaag
     6421 gctttcccac cagcgatcgg ccgcccagtg acagcacatc gaacagcatg aactcggccg
     6481 gggtctcctg ggccaggcgg cggatccggc tttcggcggg gtggaggcgc atctgcagcg
     6541 cctcgaacga caaagcgtcg cccagcggca ggatcaattc gccgtcgagc aggaagcgtt
     6601 tttcctccaa cgcctcgagc agcgcgacca cttcggggaa atagcggccg agcggcttgc
     6661 ccgagcggga ggtgagctcg acgccgccgc cgttgcgggt cgcgaggcag cggaaaccgt
     6721 cccacttggg ctcatattgc cagccggggc cgtcgggcag ctcggcggcg agcaacgcct
     6781 ccatcggcgc gggcggtctg ggcgcggcca tgggcggaca atccgcggcg ccgggatcgc
     6841 gttccgccca ggcgcgggca caaaaaaagc cccgcggaag cggggctgtt ggaacgagca
     6901 attgcgtgtt cggagacaag cgcgaggcct ggttgcgggg gcaggatttg aacctgcgac
     6961 cttcaggtta tgagcctgac gagctaccgg gctgctccac cccgcgccaa accttttgtt
     7021 gcctcaagcg ccggcgtggg catccgccca cttggctttc ctcccataag gtcgggcggc
     7081 cgttcggcct tgcggagcct acggctccga tcggcaatga aattgcgaat gggttcgaca
     7141 aggcgccggc ttcaaagcct ggcgacgacc tactcttcca ctgcttaggc agtagtacca
     7201 tcggcgctgt ccggtttcac ggccgagttc gggatgggat cgggtgggtc acggacgcta
     7261 tggccaccaa gctatggggc cggcgcattg tcggtctaga aatcgtgcac ttgggtgatg
     7321 gtattcaagc gtcatctagc tgagatcaaa ccagatcgac aggactgtcg atgatggtgg
     7381 gactctcaag cgcgaaatga gcaattagga ccggttagct tcacgcatta ctgcgcttcc
     7441 acacccggcc tatcaacgtg gtggtcttcc acggctcaaa gataccttat ctcgagggag
     7501 gcttcccgct tagatgcttt cagcggttat cccgtccatg catagctacc ctgctgcgcg
     7561 gctggcgcca cgacaggtcc accagaggca tgttca
//
LOCUS       DASTZO010000323         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_857283,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000323 DASTZO010000000
VERSION     DASTZO010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857283"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="VFU20_09160"
     CDS             <1..352
                     /locus_tag="VFU20_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286653.1"
                     /translation="ASLPKVPFVAPPGIRWVRIDRASGRRVFGTFPTREDPKSPVIWE
                     AFQPQTEPRRSYRRGQGRGEEDEEGAEDGDRGAQGGKPVRRTRAATVRQPPPAVTQPA
                     PAPSNPLPTQPPTN"
     gene            394..1521
                     /gene="prfB"
                     /locus_tag="VFU20_09165"
     CDS             394..1521
                     /gene="prfB"
                     /locus_tag="VFU20_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019515906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU5286654.1"
                     /translation="MRAEAQAHIDEISAATALLRRFLDWDRAQRRLDELNAKVEEPAL
                     WDDPKAAQEVMRERRRLEEAINATRAIERELSDTAELIEMAEAEGDEALVDEGVKSLA
                     DLAARAERDKVAALLAGEADSNNSYVEVNAGAGGTESQDWAEMLSRMYTRWAERHGMK
                     VELVDHHSGEQAGIKSATLLVRGENAYGNLKTESGVHRLVRISPYDSSARRHTSFASV
                     WVYPEVDDDIDIEVNEADLRIDTYRASGAGGQHVNTTDSAVRITHVPTGIVVACQNQR
                     SQHKNRAEAMKQLKAKLYEAELQRREAQANAQNAAKTDIGWGHQIRSYVLQPYQLVKD
                     LRTGVTSTSPGDVLDGDLDRFMAAALSQRVTGEQVEVEDVE"
     gene            1511..>2027
                     /locus_tag="VFU20_09170"
     CDS             1511..>2027
                     /locus_tag="VFU20_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5286655.1"
                     /translation="MWSKRLGLLLLAAALASCRAAPDEQRFPKPHRDVSPIIGDSFST
                     EDARDRLGEAEQVMRIAGVRPGMWVADVGAGDGYYTVRLAPAVGPRGRVLAEDIVAET
                     RNRLAQRVQRERLDNVAVRLGKPDDPLLPARSFDRIFLVHMYHEVQSPYGFLWHLRDG
                     LKPNGLVAVVDS"
BASE COUNT          340 a          735 c          706 g          246 t
ORIGIN      
        1 cgcgagcctt cccaaggtcc cgttcgtcgc cccgccgggc atccgctggg tgcgcatcga
       61 ccgggccagc ggccgccgcg tgttcggcac cttcccgacc cgggaagacc cgaagtcgcc
      121 ggtgatctgg gaagccttcc agccgcagac cgagccgcgc cgctcctacc gccggggcca
      181 gggccgcggc gaggaggatg aggagggggc cgaggacggc gaccgcggag ctcagggcgg
      241 caagccggtc cggcggaccc gcgccgcaac cgttcggcag ccgccgcccg cggtgaccca
      301 gcctgcgccc gcgcccagca atcccttgcc aacgcagccg cccactaact aggacttccc
      361 acccatccga acggggttgg aaaaggacat ttcatgcgcg ccgaggcgca ggcccatatc
      421 gacgagatca gcgccgcgac cgctctgctt cgccgcttcc tcgactggga ccgcgcgcag
      481 cggcggctgg acgagctgaa cgccaaggtc gaggagccgg cgctgtggga cgaccccaag
      541 gcggcgcagg aggtgatgcg cgagcggcgc cggctcgagg aggcgatcaa cgccacccgg
      601 gcgatcgagc gcgagctgtc cgacaccgcc gagctgatcg agatggccga agccgagggc
      661 gacgaggcgc tcgtcgacga gggcgtgaag agcctggccg acctcgccgc ccgggccgag
      721 cgcgacaagg tcgcggcgct gctcgccggc gaggccgatt ccaacaacag ctacgtcgag
      781 gtcaacgccg gcgcgggcgg caccgagagc caggactggg ccgagatgct gagccgcatg
      841 tacacccgct gggccgagcg ccatgggatg aaggtcgagc tcgtcgacca ccattccggc
      901 gagcaggcgg ggatcaagtc ggcgaccctg ctggtcaggg gcgagaacgc ctacggaaac
      961 ctgaagaccg agagcggggt ccaccggctg gtccggatca gcccctacga cagctccgcc
     1021 cggcgccaca ccagcttcgc cagcgtctgg gtctatcccg aggtcgacga cgacatcgac
     1081 atcgaggtca acgaggccga cctgcggatc gacacctacc gggcgtcggg ggcgggcggc
     1141 cagcacgtca acaccaccga cagcgcggtc cgcatcaccc acgtgccgac cgggatcgtc
     1201 gtcgcctgcc agaaccagcg ctcgcagcat aagaaccggg ccgaggcgat gaagcaattg
     1261 aaggccaagc tctacgaggc cgagctgcag cggcgcgagg cgcaggcgaa cgcccagaac
     1321 gccgccaaga ccgacatcgg ctggggccac cagatccgca gctacgtcct gcagccctac
     1381 cagctggtca aggatctgcg gaccggggtc acctcgacct cccccggtga cgtgctcgac
     1441 ggcgacctcg accggttcat ggccgccgct ttgtcgcagc gcgtgaccgg agagcaggtc
     1501 gaggtcgagg atgtggagta agcgcctagg gctgctgctc ctcgcggccg cgctcgcctc
     1561 ctgccgggcc gcgccggacg agcagcgttt ccccaagccg caccgcgacg tctcgccgat
     1621 catcggcgac agcttctcga ccgaggatgc gcgcgaccgg ctcggcgagg ccgagcaggt
     1681 gatgcggatc gccggggtcc ggccgggcat gtgggtcgcc gacgtcggcg ccggcgacgg
     1741 ctattacacc gtccggctgg cccccgcggt cggcccgcgc ggccgggtgc tggccgagga
     1801 tatcgtcgcg gaaacccgca accgcctcgc ccagcgcgtc cagcgcgagc ggctcgacaa
     1861 tgtcgcggtg cgcctcggca agcccgacga cccgctgctt cccgcccgct cgttcgaccg
     1921 catcttcctc gtccacatgt atcatgaggt gcagtcgccc tacggcttcc tctggcacct
     1981 gcgcgacggg ctgaagccca acggactcgt ggcggtggtc gactcca
//
LOCUS       DASTZO010000324         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_104300,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000324 DASTZO010000000
VERSION     DASTZO010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104300"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="VFU20_09175"
     CDS             <1..862
                     /locus_tag="VFU20_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016743781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5286656.1"
                     /translation="WGGAFFFISVAVRHVPPLTYVWLRLTIAAAGLWLFLAWRREPLA
                     LPKAAWGAILVLALFNNVVPFALFGWGQTRIASGLASILNATTPIWGVIVAHLFTRDE
                     KMSPRKLAGVGIGFGGVAVMIGPGLLATLGGELLAQLACLAATLCYAFAGVWARRFKA
                     MGVPPVTVATGQLTAGALVMLPVALILDRPWLEPLPPLSAWAAIVSLALLCTAFAYIL
                     YFRLIDSAGATNALLVTLLVPPTAILLGILFLNEVITPNEVAGMALIAVGLAAIDGRL
                     LARLRRPLPV"
     gene            complement(875..1441)
                     /locus_tag="VFU20_09180"
     CDS             complement(875..1441)
                     /locus_tag="VFU20_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5286657.1"
                     /translation="MRLILLLPGLLAPLLAITACDRQKAETPQGEAAGGPVAGQKGVD
                     RSRKGRPAPDTLFRDPDGGETSLAEFEGVPVLVNLWASWCAPCIQELPTLERLEQAHS
                     ADGRLGVIAVSQDRAPKASVDAFLADKRIARFAAFHDPDMALSSALGVEILPTTLLFD
                     SGGREVWRYVGDLDWTGAEADRLLAEAR"
     gene            1458..>2042
                     /locus_tag="VFU20_09185"
     CDS             1458..>2042
                     /locus_tag="VFU20_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HEU5286658.1"
                     /translation="MWGGRFEGGPADVMRAINSSIGTDKRLWREDVAASRAHAAMLGA
                     QGVISAKDAAAIDAGLAAIAEEYAAGGVTEDPELEDIHMHVERRLADTIGPAAGRLHS
                     ARSRNDQVATDFKLFVRGAIDEALAAIDGLETAILDRAGEHAATIMPGFTHLQSGQPV
                     TLGHHLMAYREMLARDRSRFADARRRLNECPLGAA"
BASE COUNT          263 a          731 c          730 g          318 t
ORIGIN      
        1 ctggggcggc gccttcttct tcatctccgt ggccgtccgc cacgtgccgc cgctgaccta
       61 tgtgtggctt cggctgacca tcgccgcggc cgggctgtgg ctgttcctcg cctggcggcg
      121 cgagccgctc gcgcttccca aggcagcgtg gggagcaatc cttgtcctcg ccctgttcaa
      181 caatgtcgtg cctttcgccc tgttcggctg gggccagacc cggatcgcca gcggcctcgc
      241 ctcgatcctc aacgcgacga ccccgatctg gggcgtgatc gtcgcccacc tgttcacgcg
      301 cgacgagaaa atgtcgccgc gcaagctcgc cggcgtcggg atcggcttcg gcggagtcgc
      361 ggtgatgatc ggcccgggcc tgctggcgac gctcggcggc gagctcctcg cccagctcgc
      421 ctgcctggcc gcgacactgt gctacgcgtt cgccggagtc tgggcgcggc gcttcaaggc
      481 gatgggcgtg ccgccggtga ccgtcgccac cggccagctg accgccggcg ccctcgtcat
      541 gttgccggtc gcgctgatcc tcgaccggcc gtggctcgag ccgctgccgc cgctgtcggc
      601 ctgggcggcg atcgtcagcc tggctttgct gtgcaccgcg ttcgcctaca tcctctattt
      661 ccggctgatc gattcggccg gggcgaccaa cgccctgctg gtgacgctgc tggttccgcc
      721 gaccgcgatc ctgctcggga tcctgttcct gaacgaggta atcactccca acgaggtcgc
      781 cggcatggcg ctgatcgccg ttggcctggc ggcgatcgac gggcggcttc tggcccggct
      841 gaggcgtccg ctgccggtct agggcgcttc cggcctagcg ggcctcggcg agcagccggt
      901 ccgcctcggc gccggtccag tcgaggtcgc cgacgtagcg ccacacctcg cggccgccgg
      961 aatcgaacag caaggtcgtc ggcaggattt ccaccccgag cgccgacgac agcgccatgt
     1021 ccgggtcgtg aaaggcggcg aagcgggcga ttcgcttgtc ggcgaggaag gcgtcgaccg
     1081 acgccttggg cgcccggtcc tggctgaccg cgatgacccc gagccggccg tcggccgagt
     1141 gcgcttgctc gagccgttcg agcgtcggca gctcctggat gcacggcgcg caccagctcg
     1201 cccacaggtt gaccagcacc gggacaccct cgaactcggc gaggctggtc tcgccgccgt
     1261 cggggtcgcg gaacagggtg tcgggcgccg ggcgcccctt gcggctccgg tcgacgccct
     1321 tctgcccggc gaccggcccg cccgccgcct cgccttgtgg ggtttccgcc ttttgcctat
     1381 cgcaagcggt gatcgccaac aggggcgcga gcaggccggg gagcagcagg atcagccgca
     1441 agacgatctc caatccgatg tggggcggac gctttgaagg cggcccggcc gatgtcatgc
     1501 gcgcgataaa tagctcgatc ggcaccgaca agcggctttg gcgcgaggac gtcgcggcga
     1561 gccgggcgca cgcggcgatg ctcggggcgc agggggtcat ctcggccaag gatgcggcgg
     1621 cgatcgacgc cggcctcgcc gcgatcgccg aggaatatgc ggccggcggg gtgaccgagg
     1681 acccggagct cgaggacatc cacatgcacg tcgagcggcg gctggcggac acgatcggcc
     1741 cggcggcggg gcggctgcac agcgcgcgct cgcgcaacga ccaggtggcg accgacttca
     1801 agctgttcgt gcgcggggcg atcgacgagg cgctggcggc gatcgacggc ctcgaaaccg
     1861 cgatcctcga ccgcgccggg gagcatgcgg cgacgatcat gcccggcttc acccatctgc
     1921 agtcgggcca gccggtgacc ctgggccatc acctgatggc gtaccgcgag atgctcgcgc
     1981 gcgaccgcag ccggttcgcc gacgcgcgca ggcggctcaa cgaatgcccg ctcggcgccg
     2041 cg
//
LOCUS       DASTZO010000325        10592 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_334504,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000325 DASTZO010000000
VERSION     DASTZO010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 10592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10592
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_334504"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..346
                     /locus_tag="VFU20_09190"
     CDS             <1..346
                     /locus_tag="VFU20_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286659.1"
                     /translation="ALTDPAMAEKLGRMMEALSKAFLELPVGEIEAAAEGRPATRADR
                     RRTVRELGRKDDPQFERNLERQLADSRVAMQAGMKALAAALPAMIKGQSEASRELEKA
                     TANLPSPNYPKQ"
     gene            412..1083
                     /locus_tag="VFU20_09195"
     CDS             412..1083
                     /locus_tag="VFU20_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU5286660.1"
                     /translation="MWQLFQFPLCPFSRKVRLVLAEKGVGHELVRENPWERRDEFIDL
                     NPAGETPVMVDPEAGITLIGSQPIIEYFEETVERMPMIHGNAVLRAEIRRLAQWFDEK
                     LYREVVEPLMNERMRKRLVSQESPDTRVLREAMRVANGHLDYVDYLLDHRRWLAGPGL
                     SLADFTAAAHLSVVDYLGALDWRGHKQTKDWYAVMKSRPCFRPLLGERMEVIVPPAHY
                     DKVDF"
     gene            complement(1099..1428)
                     /locus_tag="VFU20_09200"
     CDS             complement(1099..1428)
                     /locus_tag="VFU20_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286661.1"
                     /translation="MLLVPLAAAGGLALGGCVAAMAASAAGMAAQGARGRPQSNDHLK
                     PAARAECEAHAARYGSVRIIDVEQRSTSKIVVWGTVGEGEQRRSFECAYGTKVTGFKL
                     RAITPGR"
     gene            complement(1437..2159)
                     /gene="ubiG"
                     /locus_tag="VFU20_09205"
     CDS             complement(1437..2159)
                     /gene="ubiG"
                     /locus_tag="VFU20_09205"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HEU5286662.1"
                     /translation="MAQTSIVADEVEQFAKLAGDWWDPDGASAMLHKLNPVRLAYIRD
                     RINQHWQVDEHGRRPLEDRTALDVGCGAGLLAEPLARLGATVTAIDAAPELIAAAREH
                     AAGQGLKIDYCSAAVEEVEGRFDLVTSLEVIEHVADPQAFVAALAKRLAPGGLLILST
                     PNRTSWSRLLTITVAEGLGRVPKGTHDYEKFIAPERMKTLLEDAGLRCCDFEGIAWSP
                     TRGLHLSEDLRLNYLVAAVHAS"
     gene            2230..3438
                     /locus_tag="VFU20_09210"
     CDS             2230..3438
                     /locus_tag="VFU20_09210"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEU5286663.1"
                     /translation="MKFGGTSMAGIERIRNVAERVRREAEDGDEVLVVVSAMAGETDR
                     LVQLCREAAALHDPSEYDVVVASGEQVTAGLLAMTLQNMGLKARSFMGWQLLRASGVH
                     GNARVDAVESGLLDEALKGGTIAVIPGFQGMSDSGRLATLGRGGSDTSAVAIAAGVKA
                     DRCDIYTDVDGVYTTDPRIVPKARKLDAITFEEMLELAGVGAKVLQTRSVGLAMRENL
                     PLRVLSAFEDKPGTRVVAELSGADMERNQIAGIAADKNEARVTLTGIADAPGMVAKVI
                     SPLAEAGILVDMIVHAATPNTGSSDLTFTVPRASLAQAMAVIEQRRQAIGYSELIHDD
                     TVAKVSIVGVGIRSNPGLAATMFETLAERQINLLAVSSSEIKVSALIAEADLDLSVRV
                     LHTAFGLDSQ"
     gene            3435..4493
                     /locus_tag="VFU20_09215"
     CDS             3435..4493
                     /locus_tag="VFU20_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU5286664.1"
                     /translation="MSGEGLIPEARAVHQSVGHARLKQLMHRGTDFLGCDIAIMGGAM
                     SWVSECNLVSALSNAGAFGVIACGAMTPELLDTEIAETRKRTERPFGVNLITMHPQLS
                     ELIDTCAKHGVGHVVLAGGLPPGGAIDRIKGSGAKLVCFAPALSLAKKLMRSGADALV
                     IEGMEAGGHIGPVATSVLAQEILPHVAHDIPVFVAGGIGRGEAIAAYLEMGAVGVQLG
                     TRFVCATESIAHPNFKKMFIRASARDAVPSVQIDPRLPVIPVRALKNREMENFAAKQR
                     EVAQLLDSGAVEMAEAQLQIEHYWAGALRRAVIDGDVETGSVMAGQSVGMVNREEPVA
                     DIIRELADEAAAALESRG"
     gene            complement(4490..5878)
                     /locus_tag="VFU20_09220"
     CDS             complement(4490..5878)
                     /locus_tag="VFU20_09220"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEU5286665.1"
                     /translation="MIKFPDEFLWGAATSAYQIEGSPLADGAGPSIWQRFVHTPGLTK
                     GGDTGDVACDHYNRYRDDVALMRSLGLQAYRFSIAWGRIFPNGTGRVNEAGLDFYERL
                     VDSLLEAGIEPLATLYHWDLPAALDDRGGWLNPDIAGWFADYAEAVFRRLDGRVKKWA
                     TLNEPWVVTDGGYLHGALAPGHKNLFETPIASRNLMLAHGAAVKRYREVGAHDIGLVV
                     NLEPKYPASDDPADRAAADRSAAYMNQQYLDPAYGLGCPPAMAEIFGAGWRDWTEEEL
                     ALAAQPTDWLGINYYTRGVMRHDPSRWPVMADYQRQPGKTYTDTGWEVFEQGLTDTLL
                     WVKERYGNPPVYITENGSAFYDPPVATDGAVHDPLRVDYLKRHLRAVHEAIAKGADVR
                     GYMAWSLLDNLEWSLGFAKRFGIVHVNYETQERTPKDSALLYSQVIASNGGVLGGDRL
                     GEAVRPKADAIV"
     gene            complement(5892..8588)
                     /locus_tag="VFU20_09225"
     CDS             complement(5892..8588)
                     /locus_tag="VFU20_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286666.1"
                     /translation="MRRLAALPALALLCAATAPVFIDPMDDASNWPAKASDSVAVSGA
                     AVPGAIGRAVELRYDFGRVSGYAFMRRPVALELPRNYEVRFRIRGSGGRNDLQMKLTH
                     GDNVWWKVWRNYRPTDGWREVVVPAGEIGFAWGPIEDKTLRRADGIEFVVARNRDGGA
                     GHIAIDDLRIVPLAGAPAVARPPEESANDRLAGQAKASPRGAFPRAFLGEQPYWTLAA
                     SDGGKVAALISEDAAIEPAKGSYSIEPVIIDGGKRIDWAAVRPSHSLADGKLPIPSVT
                     WTAPGLRLDTTLLADSAGRAALARYALTNTGGARRTVELRLGIRPWQVNPPAQFLAQQ
                     GGASPIARIVRDGARLAITQPQTEGDPPLTRTLIASRPPDSAAIGALAGKDMATADLV
                     YRLDLAPGAGDEIVLTMPGAGGAAPSWDESLAATRAHWREVLGRVELRVPPAKRPVAD
                     TLATAFAQILTSRDGPMLKPGTRSYDRAWIRDGAMMSEALLRMGRADVARAFADWYRP
                     NLFDSGKVPCCVDFRGADPVPENDSHGQYIFLVSQLYRFTGDRAALARDWPAVLAATR
                     YMDQLRSSERTPANLVPGRRMLYGLMPPSISHEGYSAKPQYSLWDDFWALRGYRDSAD
                     IARTLGKPEAAAIAASRDQFAADLHAAILAARDHWKIDFIPGATSLGDFDATSTTIAL
                     DPANEQARLDRKMLDATFERYWRDFQARAAGTRAWKDYTPYETRTIGSFVRLGWRDRI
                     DPLLDFFMASRRPAEWNQWAEVVGRDPREVRFIGDMPHAWVASDFVRGVLDMFAWERR
                     DDSALVLGGGLSAGWLAGAGSSIAGLATPYGRLDFSMRGDARNLVATVGGSARPPGGF
                     VLAWPFAGTPPPARVDGRAVPWRNRAITFTATGKPIRIRVGR"
     gene            complement(8585..9409)
                     /locus_tag="VFU20_09230"
     CDS             complement(8585..9409)
                     /locus_tag="VFU20_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5286667.1"
                     /translation="MRSPPSARVARIVLLVILAGFALFPLLWMLSVSLMAPGEASQFP
                     PPLLPDAPGLSAYRALFATTGIGIYFINSLIVATLGTLLSLAFNATAGYAFAKLKFRG
                     RDRIFQLLLAALVIPAQVTMLPLFLMLKEIGLINSFAGVLVPVTASIFGIFLVRQYAL
                     SIPDELLEAARIDGASEGRIFLQIVVPALTPILVTLALFTFLGVWNDFMWPLIVLTDQ
                     DKYTLPVALAALSREHVQDNELMMAGAVLTILPVLLLFLVLQRYYMQGLLAGSVKG"
     gene            complement(9409..10290)
                     /locus_tag="VFU20_09235"
     CDS             complement(9409..10290)
                     /locus_tag="VFU20_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010215023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5286668.1"
                     /translation="MSRYSAAGWAFAGPALVMLSLFFFIPVLLAFVLSLTDFDLYALA
                     DIQDLRFIGLGNYGELLQTPLFWKALGNTFYFVIVGVPLSIALSLAAAMLLNGVASAL
                     VGLFRTALFAPVVTTLVAVAVIWRYLLHTRYGMINYGLERLGIDPVDWLGDPNWAMPA
                     IIFFAVWKNFGYNMVILLAGLQTIPNDLYEAARIDGANAWQRFLHVTLPGLGPMLLLV
                     SILTMAGYFQLFAEPYVMTQGGPVESTVSVLYFMYEQGFKWWNLGFASAVAFILFVIM
                     FVITAIQYRLAKARGVA"
     gene            complement(10287..>10592)
                     /locus_tag="VFU20_09240"
     CDS             complement(10287..>10592)
                     /locus_tag="VFU20_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5286669.1"
                     /translation="RPEVQVRFFELTGDLPPRRSAWAAPLLARDPYAKAFEEQLERTE
                     APPAVPEWERIGQQMRIVSERAVAGQITPAQFRATLDADTGEMLEKRRWKLAREAAR"
BASE COUNT         1693 a         3628 c         3728 g         1543 t
ORIGIN      
        1 ggcgctgacc gacccggcga tggccgaaaa gttggggcgg atgatggagg cgctgtccaa
       61 ggcgttcctc gagctgccgg tcggcgagat cgaggccgcc gccgagggcc gcccggcgac
      121 ccgcgccgac cgccgccgaa cggtgcgcga gctgggccgc aaggacgacc cccagttcga
      181 aaggaacctc gagcggcagc tcgccgacag ccgggtcgcg atgcaggccg ggatgaaagc
      241 cctcgccgcc gcgctgccgg cgatgatcaa ggggcagagc gaagcctcgc gcgagctcga
      301 gaaggccacc gccaacctgc cgtcgcccaa ttatcccaaa cagtagctcg gcgttaaagg
      361 ccggccaacg cgcctagacg cgctcgcccg cgccgctaaa gctggccgct gatgtggcag
      421 ctgttccaat tcccgttgtg cccgttctcg cgcaaggtcc ggctggtgct cgccgaaaaa
      481 ggcgtcgggc acgagctggt gcgcgagaac ccgtgggagc ggcgcgacga gttcatcgac
      541 ctgaaccccg ccggcgagac cccggtgatg gtcgaccccg aggccgggat cacgctgatc
      601 ggaagccagc cgatcatcga atatttcgag gagacggtcg agcgcatgcc gatgatccac
      661 ggcaatgcgg tgcttcgcgc cgaaatccgg cgcttggccc aatggttcga cgaaaagctc
      721 taccgcgagg tcgtcgagcc gctgatgaac gagcgcatgc gcaagcggct ggtcagccag
      781 gaatcgccgg acacgagagt gctccgcgag gcgatgcggg tcgccaacgg ccacctcgac
      841 tatgtcgact atctgctcga ccaccgccgc tggctggccg ggcccggcct cagcctcgcc
      901 gacttcaccg ccgccgcgca cctgtcggtc gtcgattatc tcggtgcgct cgactggcgt
      961 ggccacaagc agaccaagga ctggtacgcg gtgatgaagt cgcggccctg cttccggccg
     1021 ctgctcggcg agcggatgga ggtgatcgtc ccgcccgcgc attacgacaa ggtggatttc
     1081 taggcgggct ctcgcggttc agcggccggg cgtgatcgcg cggagcttga agcccgtgac
     1141 cttggtcccg taggcacatt cgaacgagcg gcgttgttcg ccctcaccga ctgtccccca
     1201 gacgacgatc ttgctggtgc tgcgctgctc gacgtcgatg atgcgcacgc tgccgtatcg
     1261 cgccgcatgg gcctcgcact cggcgcgggc ggcgggcttc agatggtcat tgctctgggg
     1321 ccggccgcgc gcgccctgcg cggccatgcc cgcggcgctg gcggccatcg cggccacgca
     1381 cccgcccaga gcaaggccgc ccgcggcggc cagcggcaca agcagcattc ggcgcctcac
     1441 gaggcgtgca ccgccgccac gaggtaattc agcctcaggt cctcgctgag gtgcaggccg
     1501 cgggtcggcg accaggcgat tccttcgaaa tcgcagcacc gcagcccggc gtcctcgagc
     1561 agcgtcttca tccgctcggg cgcgatgaac ttctcgtagt cgtgggtgcc tttggggacc
     1621 cggcccagcc cttccgcgac cgtgatcgtc agcagcctcg accagctcgt ccggttgggg
     1681 gtcgacagga tgagcagccc gccgggcgcc agccgcttcg ccagcgcggc gacgaacgcc
     1741 tgcgggtcgg cgacatgctc gatcacctcc aatgaggtga cgaggtcgaa ccggccttcg
     1801 acctcctcga ccgcggcgga gcaatagtcg atcttaagcc cctgccccgc ggcatgctcg
     1861 cgggcggccg cgatcagctc cggcgcggcg tcgatcgcgg tgacggtcgc gcccagccgg
     1921 gcgagcggct cggcgagcag cccggcgccg cagccgacgt cgagcgccgt cctgtcctcg
     1981 agcggccgcc ggccgtgctc gtccacttgc cagtgctggt tgatccggtc gcggatgtag
     2041 gcgagccgca ccgggttcaa tttgtgcagc atcgccgacg cgccgtccgg atcccaccag
     2101 tcgccggcca gcttggcgaa ctgctcgacc tcgtcagcca cgatgcttgt ctgcgccatg
     2161 tgcgcctcct aacagggcgc gccggttagc ccaaggagcc gcatggcccg cccaaaaaac
     2221 cgcatcgtga tgaaattcgg cggcacgtcg atggccggca tcgagcgcat tcgcaacgtc
     2281 gccgagcggg tccggcgcga ggccgaggac ggcgacgagg tgctggtggt cgtctcggcg
     2341 atggccggcg agaccgaccg gctggtgcaa ttgtgccgcg aggccgccgc cctccacgac
     2401 ccgagcgaat atgacgtcgt cgtcgcgtcc ggcgagcagg tgaccgcggg gctgctggcg
     2461 atgaccctcc agaacatggg cctgaaggcg cgcagcttca tgggctggca gctgctccgc
     2521 gcgtcagggg tccatggcaa cgcccgcgtc gacgcggtcg aatccgggct tctcgacgag
     2581 gccctgaagg gcggcacgat cgccgtcatc cccggcttcc agggcatgtc cgactccggc
     2641 cgcctggcga cgctgggccg cggcgggtcg gacacgtcgg cggtcgccat cgccgccggg
     2701 gtcaaggccg accgctgcga catctacacc gacgtcgacg gcgtctacac gaccgatccg
     2761 cggatcgttc ccaaggcgcg caagctggac gcgatcacct ttgaggagat gctcgagctg
     2821 gcgggcgtcg gcgccaaggt gctccagacc cgctcggtcg ggcttgcaat gcgggagaat
     2881 ttgccgctcc gggtgctgtc ggcgttcgag gacaagcccg gaacgcgcgt cgtcgccgag
     2941 ctttcaggag ccgacatgga gagaaaccag atcgccggga tcgcggccga caagaacgag
     3001 gcgcgggtga ccctgaccgg gatcgccgac gccccgggca tggtcgccaa ggtgatcagc
     3061 ccgctcgccg aggccggcat cctggtcgac atgatcgtcc acgcggcgac ccccaacacc
     3121 ggctcgagcg acctcacctt caccgtgccg cgcgccagcc tcgcccaggc gatggcggtg
     3181 atcgagcagc gccggcaagc gatcggctat tccgagctga tccacgacga caccgtcgcc
     3241 aaggtcagca tcgtcggggt cggcatccgc tccaaccccg gcctcgcggc gacgatgttc
     3301 gagaccctgg ccgagcggca gatcaacctg ctcgcggtgt cctcctccga gatcaaggtc
     3361 agcgccctga tcgccgaggc cgatctcgac ctctccgtgc gcgtgctcca caccgcgttc
     3421 gggctggact cgcaatgagc ggcgaagggc tgatcccgga agcaagagcg gtccaccaaa
     3481 gcgtcggcca cgcccggttg aagcagctga tgcaccgcgg caccgacttc ctcggctgcg
     3541 acatcgccat catgggcggg gcgatgagct gggtcagcga gtgcaacctc gtctcggccc
     3601 tgtccaatgc cggagcgttc ggggtgatcg cctgcggggc aatgaccccc gagctgctcg
     3661 acaccgagat cgccgagacc aggaagcgca ccgagaggcc cttcggcgtc aacctcatca
     3721 ccatgcaccc gcaattgagc gagctgatcg acacctgcgc gaagcatggc gtcggccacg
     3781 tcgtgctcgc cggcgggctt cccccgggcg gagcgatcga ccgcatcaag ggcagcggcg
     3841 cgaagctcgt gtgcttcgcc ccggccctgt cgctcgcgaa gaagctgatg cgctcgggcg
     3901 ccgacgcact ggtgatcgag ggcatggagg ccggtggcca catcggcccg gtcgcgacct
     3961 cggtgctggc gcaggagatc ctgccgcacg tcgcccacga tattccggtg ttcgtcgccg
     4021 gcgggatcgg ccgcggcgag gcgatcgccg cctatctcga gatgggcgcg gtcggtgtcc
     4081 agctcggcac ccgcttcgtc tgcgcgaccg agagcatcgc ccaccccaat ttcaagaaga
     4141 tgttcatccg cgcgtcggcg cgcgatgcgg ttcccagcgt ccagatcgac ccgcggctgc
     4201 cggtgatccc ggtccgcgcg ctcaagaacc gcgagatgga gaatttcgcc gccaagcagc
     4261 gcgaggtcgc gcaactgctc gactccggcg cggtcgagat ggccgaggcg cagctgcaga
     4321 tcgagcatta ttgggcgggc gcactgcgcc gcgcggtgat cgacggcgac gtcgagaccg
     4381 gatcggtgat ggccggccag tcggtcggga tggtgaaccg cgaggagccg gtcgccgaca
     4441 tcatccgcga gctcgccgac gaggcggccg cggcgctcga gagccgcggc tagacgatcg
     4501 cgtccgcctt gggccggacg gcctcgccca accggtcgcc gccgaggacg ccgccgttgc
     4561 tggcgatcac ctgcgagtag agcaaggcgc tgtccttcgg cgtccgctcc tgcgtttcgt
     4621 aattgacgtg gacgatcccg aagcgcttcg cgaagccgag cgaccattcg aggttgtcga
     4681 gcagcgacca ggccatgtag ccgcggacgt cggcgccctt ggcgatcgcc tcgtgaacgg
     4741 cgcgcaggtg ccgcttcaga taatcgaccc gcagcgggtc gtgcaccgct ccgtcggtcg
     4801 ccaccggcgg atcgtagaag gccgagccgt tctcggtgat gtagaccggc gggttgccgt
     4861 agcgctcctt cacccacagc aaggtgtcgg tcaggccctg ctcgaacacc tcccagccgg
     4921 tgtcggtgta ggtcttgccg ggctggcgct ggtagtcggc catcaccggc cagcgcgacg
     4981 ggtcatgcct catcaccccg cgcgtgtaat aattgatccc gagccagtcg gtgggttggg
     5041 cggccagcgc tagctcttcc tcggtccagt cgcgccaccc ggcgccgaag atctcggcca
     5101 tcgccggcgg gcagccgagg ccgtaggcgg ggtcgaggta ctgctggttc atataggctg
     5161 ccgagcggtc ggcggcggcg cggtcggccg gatcgtccga cgcgggatat ttgggctcca
     5221 gattgaccac cagcccgatg tcgtgggcgc cgacctcgcg gtagcgcttc accgcggcgc
     5281 cgtgcgcgag catcagattg cgcgaggcga tcggcgtctc gaacagattc ttgtgccccg
     5341 gagcgagcgc gccgtgcagg tagccgccgt cggtgaccac ccacggctcg ttgagggtcg
     5401 cccatttctt cacccggccg tcgagccgcc ggaacaccgc ctcggcataa tcggcgaacc
     5461 agccggcgat gtccgggttg agccagccgc cgcggtcgtc gagcgccgcc ggcaggtccc
     5521 agtggtagag cgtcgccagc ggctcgattc cggcctcgag caggctgtcg accagccgct
     5581 cgtagaaatc gagcccggcc tcgttcaccc gcccggtgcc gttcgggaag atgcggcccc
     5641 aggcgatgct gaagcggtag gcctggagcc ccagcgagcg catcagcgcg acatcgtcgc
     5701 ggtagcggtt gtaatggtcg caggcgacgt cgccggtgtc acctcccttg gtgagcccgg
     5761 gggtatggac gaagcgctgc cagatgctcg ggccggcgcc gtcggcgagc ggcgagcctt
     5821 cgatctggta ggccgaggtc gccgcgcccc acaggaattc gtcggggaat ttgatcattt
     5881 cttgtccttg ttcatcgccc cacccggatc cggatgggtt tgcctgtcgc ggtaaaggtg
     5941 attgcccggt tgcgccatgg cacggcgcgg ccatcgaccc gcgccggcgg cggagtgccg
     6001 gcgaacggcc aggcgagcac gaaaccgccg ggcgggcgcg cgctgccgcc gaccgtcgcg
     6061 accaggttgc gggcgtcgcc gcgcatcgaa aaatcgagcc ggccgtaggg agtcgcaagc
     6121 ccggcgatgc tcgagccggc tcccgccagc cagcccgccg acaggccgcc gccgagcacc
     6181 agcgcggaat cgtcgcgccg ctcccaggcg aacatgtcga gcaccccgcg gacgaagtcg
     6241 gatgcgaccc aggcgtgcgg catgtcgccg atgaagcgga cttcgcgcgg gtcgcggccg
     6301 accacctcgg cccactggtt ccattcggcc gggcgccggc tggccatgaa gaagtcgagt
     6361 aggggatcga tccgatcgcg ccagccgagg cggacgaagc tgccgatggt ccgggtctcg
     6421 tagggcgtgt agtccttcca cgcgcgcgtt cccgcggcgc gggcctggaa atcgcgccag
     6481 tagcgctcga acgtcgcgtc gagcatcttg cgatcgagcc gggcctgctc gttggccggg
     6541 tcgagcgcga tggtcgtcga ggtcgcgtcg aagtcgccga ggctggtcgc gccggggatg
     6601 aaatcgatct tccaatggtc gcgcgcggca aggatcgcgg cgtgaaggtc ggcggcgaac
     6661 tggtcgcgcg acgcggcgat cgccgccgcc tcgggcttgc cgagcgtgcg cgcgatatcg
     6721 gccgagtcgc ggtagccgcg aagcgcccag aaatcgtccc acaggctgta ttgcggcttg
     6781 gccgaatagc cttcgtggct gatcgacggc ggcatcagcc cgtagagcat ccgccgcccg
     6841 gggacgaggt tcgccggcgt ccgctcgctc gagcgaagct ggtccatgta gcgcgtcgcg
     6901 gcgagcacgg cgggccagtc gcgggcgagc gcggcgcggt cgccggtgaa ccggtagagc
     6961 tggctgacga ggaagatata ttggccgtgg ctgtcgttct ccggcaccgg gtcggcgccg
     7021 cggaaatcga cgcagcacgg gaccttgccg ctgtcgaaca ggttgggccg gtaccagtcg
     7081 gcgaacgccc gcgcgacgtc ggcccggccc atccgaagca gcgcttcgga catcatcgcg
     7141 ccgtcgcgga tccaggcgcg gtcgtagctg cgggtgccgg gcttgagcat cggcccgtcg
     7201 cggctggtga ggatctgcgc aaaagccgtc gcgagcgtgt cggcgaccgg ccgcttggcc
     7261 ggcgggacgc gcagctccac ccgcccgagc acctcgcgcc aatgcgcccg ggtggcggcc
     7321 agcgattcgt cccacgacgg cgccgcaccg ccggcgcccg gcatcgtcag gacgatctca
     7381 tcgccggccc ccggcgccag gtcgaggcga tagacgaggt cggcggtcgc catgtccttg
     7441 ccggcaagcg cgccgatcgc cgccgagtcc ggcggccggc tggcgatcag ggtgcgggtg
     7501 agcggcgggt cgccttcggt ctgcggctgg gtgatcgcca ggcgggcgcc gtcgcgcacg
     7561 atccgcgcga tcgggctcgc gccgccctgc tgggcgagga attgcgccgg cgggttgacc
     7621 tgccacggcc ggatcccgag ccgcagttcg acggttcggc gagcgccgcc ggtgttggtc
     7681 agcgcatagc gggcgagcgc cgcccggccg gcgctgtcgg cgagcagggt cgtgtcgagg
     7741 cgaaggcccg gagcggtcca ggtcaccgac gggatcggca atttaccgtc ggcgagcgag
     7801 tggcttggcc ggacggccgc ccagtcgatc cgcttgccgc cgtcgatgat gaccggctcg
     7861 atcgaatagc tgcccttggc gggctcgatc gcggcgtcct cgctgatcag cgcggcgacc
     7921 ttgccgccgt cgctagccgc cagagtccag taaggctgct cgccgaggaa ggcgcgcggg
     7981 aaggcgccgc gcgggctcgc cttggcttgg ccggcgaggc ggtcgttggc cgattcttcc
     8041 ggcggccggg ccacggccgg ggctccggcg agcgggacga tgcgcaggtc gtcgatcgcg
     8101 atatgcccgg cgccgccgtc gcgattgcgg gcgacgacga actcgatccc gtcggcgcgg
     8161 cgcagcgtct tgtcctcgat cggcccccag gcgaagccga tctcgccggc gggcacgacg
     8221 acctcccgcc acccgtcagt cggccggtag ttgcgccaca ccttccacca cacattgtcg
     8281 ccgtgggtca gcttcatctg caggtcgttg cggccgccgc tgcccctgat ccggaaccgg
     8341 acctcgtaat tgcgcggcaa ttcgagcgcc accgggcggc gcatgaacgc gtagcccgac
     8401 acccggccga agtcgtagcg cagctcgacc gcgcggccga tcgcgccggg caccgcggcg
     8461 ccggacaccg cgacgctgtc ggacgccttc gccggccagt tcgatgcgtc gtccatcggg
     8521 tcgatgaaaa ctggcgcggt cgccgcgcac agcagggcga gcgccggcaa tgccgcgagc
     8581 cgcctcaccc cttgacgctc ccggccagca ggccctgcat gtaatagcgc tgaaggacca
     8641 ggaacagcag cagcaccggc aggatggtca gcaccgcccc cgccatcatc agctcattgt
     8701 cctggacatg ctcgcgcgac agcgccgcga gcgccaccgg cagcgtgtac ttgtcctggt
     8761 cggtgagaac gatcagcggc cacatgaagt cgttccacac gccgaggaag gtgaacagcg
     8821 ccagcgtgac caggatcggg gtcagcgccg ggaccacgat ctggaggaag atgcggccct
     8881 cgctggcgcc gtcgatgcgc gccgcctcga gcagctcgtc ggggatcgac agcgcgtatt
     8941 gccgaaccag gaaaatgccg aagatgctgg ccgtcaccgg caccagcacg cccgcgaagc
     9001 tgttgatgag gccgatctcc ttgagcatca ggaacaaggg cagcatcgtc acctgcgccg
     9061 ggatcaccag agcggcgagc agcagctgga agatccggtc gcggccgcgg aacttcaact
     9121 tggcgaacgc gtagccggcg gtcgcgttga acgcgagcga cagcagggtg cccaacgtcg
     9181 cgacgatcag gctgttgatg aaatagatgc cgatcccggt ggtcgcgaac aaggcccggt
     9241 aggcgctcag ccccggcgcg tcggggagca gcggcggcgg gaactggctc gcttcgcccg
     9301 gagccatcag cgacaccgac agcatccaca gcaacgggaa caaggcgaag cctgcaagga
     9361 tgaccagcag cacgatccgg gccacccggg ccgatggcgg gctgcgcatc aggcgactcc
     9421 ccgcgccttg gcgagccggt actggatcgc ggtgatgacg aacatgatca cgaacaggat
     9481 gaaggcgacc gcggacgcga agccgaggtt ccaccatttg aagccctgct cgtacatgaa
     9541 atagaggacg ctgacggtgc tttcgaccgg cccgccctgg gtcatcacat agggctcggc
     9601 gaacagctgg aaataaccgg ccatggtcag gatcgagacc aaaaggagca tcggcccgag
     9661 gcccgggagg gtgacgtgga ggaagcgctg ccaggcgttg gcgccgtcga tccgcgccgc
     9721 ttcgtagagg tcgttgggga tcgtctgcag cccggccagc aggatcacca tgttgtagcc
     9781 gaagttcttc cacaccgcga agaagatgat cgccggcatc gcccagttcg ggtcgccgag
     9841 ccagtcgacc gggtcgatcc ccagtcgctc gagcccgtag ttgatcatcc cgtagcgggt
     9901 gtggagcaga tagcgccaga tcaccgcgac cgcgaccagg gtggtgacca ccggagcaaa
     9961 caaggcggtt cggaacaagc cgaccagggc cgaggcgacc ccgttcagga gcatcgccgc
    10021 ggcgagcgac aaggcgatcg acagcggcac tccgacgatg acgaaataga aggtgttgcc
    10081 gagcgccttc cagaacaagg gcgtctgcag cagctcgccg taattgccca ggccgatgaa
    10141 ccgcaggtcc tggatgtcgg ccagcgcgta aaggtcgaag tcggtcaagc tgaggacgaa
    10201 ggcgagcagc accgggatga agaagaacag gctcagcatc acgagcgccg ggcccgcgaa
    10261 cgcccagccg gcggccgagt agcggctcat cgggcggctt cccgggcgag cttccagcgg
    10321 cgcttttcga gcatttcgcc ggtgtcggcg tcgagcgtcg cccggaattg cgccggagtg
    10381 atctgcccgg cgaccgcgcg ctccgagact atccgcatct gctggccgat gcgctcccat
    10441 tcggggaccg cgggcggcgc ctcggtgcgc tcgagctgct cctcgaacgc cttcgcataa
    10501 gggtccctgg cgagcagcgg cgcggcccac gcgctgcgcc tcggcggcaa gtcgccggtc
    10561 agctcgaaga agcgcacctg cacctcgggc cg
//
LOCUS       DASTZO010000326         2009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_858356,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000326 DASTZO010000000
VERSION     DASTZO010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_858356"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="VFU20_09245"
     CDS             <1..532
                     /locus_tag="VFU20_09245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286670.1"
                     /translation="WPLGSAPIEEATKANVTAQLLMPGGNTSAPCRYDPDKVELYAWR
                     HYEEGRQSPAPAVAVPSPTGPAQATTASPSPAPARVRGEPKREKDPVRLATGIKRMAT
                     EGDLSYPVWDFNGVDVSYARTGDESRWLELFVVASNGDGDDKVEIKMDPAPWLYGGPD
                     NKNWQEPRPLPSESCR"
     gene            721..>2009
                     /locus_tag="VFU20_09250"
     CDS             721..>2009
                     /locus_tag="VFU20_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEU5286671.1"
                     /translation="MDDRDLAFMPATELRRRICQREVSPVEVVEQALARIERVNPVVN
                     AYCTVAAEQARAAARAAEAAVVRGDALGPLHGVPVAIKDMSLTAGIRTTFGSRLYADY
                     VPTEDSLDIARLKQAGAVVIGKTNTPELAAGPNTVNVLFGATRNPWNLERSAGGSSGG
                     SAVALACGLTPLATGGDLGGSLRIPASVCGVVGFRTSPGRIPMYPSSWTSESFLVVGP
                     MARTVRDTALLLAATAGPDDRVPISLDEPGSVFEYAADGGVRGFRVAWSPDLGVGRVD
                     PEVARIVEAACRRFEAAGCSVDAAQPEIGEIGPMIATYRALGAATGLPELLDRADEVD
                     NPLLREFLKRPRELTGLQIAQAGIDHARYLERMAAFFRQYDLLVTPTTPTAAYMLDQM
                     YPPEIDGEPVANALDAMLMTYAFTMAGLPAISVPAGTT"
BASE COUNT          300 a          699 c          715 g          295 t
ORIGIN      
        1 ctggccactc ggcagcgcgc cgatcgagga agcgacgaag gcgaatgtca cggcgcagtt
       61 gctgatgccg ggcggcaaca cctcggcgcc gtgccgctac gacccggaca aggtcgagct
      121 gtacgcctgg cgccactacg aggagggcag gcaatccccg gcgccggccg tggcggtccc
      181 ctccccgaca ggaccggcgc aagcgacgac cgcctcgccg tctccggcac cggcgcgggt
      241 gcggggtgag ccaaagcgcg agaaggatcc ggtccggctg gcgaccggca tcaagcggat
      301 ggcaaccgaa ggcgacctca gctatcccgt ctgggacttc aacggcgttg acgtgtcgta
      361 cgcccgcaca ggcgacgaga gccggtggct ggagttgttc gtggtcgcca gcaatggcga
      421 cggcgacgac aaggttgaga tcaagatgga cccggcgccc tggctctacg gcggcccgga
      481 caacaagaac tggcaggagc cgcgtccgct gccctcggag agctgccgct aaacttcgac
      541 ggcaatcgcc ccttaccccc atcccgctgc gcggcgcgaa tatcggcttc gatgtcgtgc
      601 gaagcacgat agtcgcggcc cgcagggccg acatcatctc ccggtgtccg ggagcggggt
      661 gccatcggca agctcgccgg cgtgctaccc tcccgacagc actgcgcgtt gaggatgtcc
      721 gtggacgacc gtgatctcgc tttcatgccc gcgaccgagc tgcgccgccg gatctgtcaa
      781 cgcgaggtct cgccggtcga ggtcgtcgag caggcgctag cccgcatcga gcgggtcaat
      841 ccggtcgtca acgcctactg cacggtggcc gccgagcagg cccgggccgc cgcccgcgcg
      901 gccgaagcag ccgtcgtgcg cggcgacgcg ctcgggccgc tccacggcgt gccggtcgcg
      961 atcaaggaca tgagtctgac ggccggcatc cgcaccacat tcggctcgcg gctgtacgcc
     1021 gactacgtgc ccaccgagga ctcgctcgac atcgcgcggc tgaagcaggc cggcgccgtc
     1081 gtcatcggca agaccaacac gccggagctc gcggctggcc cgaacaccgt caacgtcctg
     1141 ttcggtgcca cgcgcaatcc ctggaacctg gagcgctcgg ccggcggatc gtccggtggc
     1201 tcggcggtgg cgctggcgtg cggcctgacg cccctcgcca cgggtggcga cctgggcgga
     1261 tcgttgcgga ttccggcgag cgtctgcggg gtcgtcggat tccgcacgtc gccggggcgc
     1321 atcccgatgt acccgtcgag ttggacctcc gagtcgttcc tggtggtcgg cccgatggcg
     1381 cgcaccgtgc gcgatacggc gctgctgctg gcggcgacgg ccgggccgga cgaccgcgtg
     1441 ccgatctccc tggacgagcc gggctctgtc ttcgagtacg cggctgatgg cggtgtccgc
     1501 gggttccgcg tggcctggag cccggacctc ggcgtgggcc gggtcgatcc ggaggtggcg
     1561 cgcatcgtcg aggcagcgtg ccgtcgattt gaagcggcgg gctgttcggt ggacgcggct
     1621 cagccggaga tcggcgagat cggcccgatg atcgcgacct accgggcgct cggggcggcg
     1681 accggcttgc ctgagctgct ggaccgcgcc gacgaggtgg acaatccgct cctgcgcgag
     1741 ttcctgaagc ggccacgcga gctgaccggc ctccagatcg cgcaggccgg catcgaccat
     1801 gcgcgctacc tggagcggat ggcggcgttc ttccgccagt acgatctgct ggtcacgccg
     1861 accacgccga ccgccgccta catgctggat cagatgtacc cgcccgagat cgacggcgag
     1921 ccggtcgcca atgccctcga cgcgatgctg atgacatacg cgtttacgat ggccggcctg
     1981 cccgcgatat cggtgccagc cggtacgac
//
LOCUS       DASTZO010000327         3900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_204590,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000327 DASTZO010000000
VERSION     DASTZO010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_204590"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VFU20_09255"
     CDS             complement(<1..254)
                     /locus_tag="VFU20_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286672.1"
                     /translation="MSVARHTAYNFIGAVIPLAVSLVTVPLYLQVIGLDRYGVLAICW
                     LLVGYFMLFDFGLGRATAQKIATLAHSPAAERNRIFWTGAA"
     gene            344..1771
                     /locus_tag="VFU20_09260"
     CDS             344..1771
                     /locus_tag="VFU20_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HEU5286673.1"
                     /translation="MALSRRQILERSAAAASLALIPGRVRAAAARAAALTPEMFGARG
                     DGVTNDTLALARLASAVNSNGGGEIAFGAGKTYRVGLQSNSGPLAHLYAFQPHDILHF
                     KGCTRPLVIRGNGAVLKCAPGLRYGTFDRAGRPTRHRLPFYTPGELATPYQYMIKIES
                     CRGPIALSDIELDGGLAGLRIGGEYGDHGRQIPATGLGLFNNSGSETIANVHTHHHGQ
                     DGLIIDGIARPVPGAVRQVTALRSEYNGRQGCSIVGGRGYVFSDCKFNHTGQAGIQSA
                     PAAGVDIEAEGDKINRDFRFVNCEFVNNGGAGLLADSGDSEGVVAEQCRFVGSSSWAA
                     WPRKPRMRFTNCTFVGQIVQCHGDPDQSRAAQFHSCRFYDDPKLSPTGKLWASGGANA
                     VADLGAGGENVLFSKCIFELTHRLVLPWSWHAIYSDCRMRQKSPQIAYPKGTYLGSST
                     IWGKVDMYGTKVRGRLVVNGVPLRG"
     gene            1780..3771
                     /gene="asnB"
                     /locus_tag="VFU20_09265"
     CDS             1780..3771
                     /gene="asnB"
                     /locus_tag="VFU20_09265"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010185639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU5286674.1"
                     /translation="MCGIAGIIASSRLEERDVRRLIDPITHRGPDDEGVWIDAEAGVG
                     FGHRRLAILDLSPAGHQPMTSADGRYVITYNAEIYNHAELRAELEAAGAAPPGGWRGH
                     SDTETLLECVSAWGLAETLPRIVGMFAFALWDRQERQLHLVRDRFGEKPLYYGWAGRD
                     FLFASELKAIRAHPRFDGSIDRRALKLFAARTYIPAPLSIYRRLFKLEPGCVLTVTPE
                     AAKTPLDAPPQVDGPAGAIRIRRYWSYRDVMRRGLEQPIGDEGEALDRLEQVLATAIR
                     GQSVADVPVGAFLSGGIDSSAIVALYQKYSSVPVRTFTIGFEEAGFNEAEDAKKVAAH
                     LGTVHSERYVTVKEARDVIPLLPAMYDEPFADSSQIPTFLVSRFAREQVTVALTGDGG
                     DELFAGYNRHFAAPQLWQQLRWLPRPLRSAIGSPLSRLPSQFWNGAVGLLSGRRRPHL
                     GGKIQKGLRVAGSVRNFDELYSSFLDEWSFEDSPVRGGDGAGPGWDMDIADQAPDTLR
                     MMYCDAVSYLPDDILCKVDRASMAVSLETRVPYLDHRVAELAARIPLELKVRGGSGKH
                     ILRELLYREVPRELFERPKAGFAIPVGEWIKGSLRPWAEDLLDPSRMAGEGWFDPAIV
                     QRRWKEHLGGRRDSTPALWAVLMFQSWLREQRQPLSAAA"
BASE COUNT          612 a         1370 c         1327 g          591 t
ORIGIN      
        1 gccgccccgg tccagaagat ccggttccgc tcggccgccg gcgaatgggc cagggtcgcg
       61 atcttctggg cggtcgctcg cccgagcccg aaatcgaaca gcatgaaata gccgaccagc
      121 agccagcaga tggcgagcac cccgtagcgg tcgagcccga tgacctggag gtatagcggc
      181 acggtgacca gcgacaccgc gagcgggatg accgcgccga tgaaattata agcggtgtga
      241 cgggcgacgc tcatcgcggc cgagcttaga ccagcgcggc caaggcaccg caagcctccg
      301 ccttctggtg gcgggacggc caaatgtgcc aaagggcgcg gcgatggcac tctcccgccg
      361 ccagatcctc gagcgctcgg cagccgcagc ctcgctcgcg ctgattcccg ggcgagtccg
      421 ggcggccgcc gcccgggccg cggccctgac gcccgaaatg ttcggcgccc gcggcgacgg
      481 ggtcaccaac gacacattgg cgctcgcccg gctggcttcg gcggtgaact cgaacggcgg
      541 cggggaaatc gcgttcggcg ccggcaagac ctatcgggtc gggctccagt cgaactccgg
      601 cccgctcgcc cacctctacg cgttccagcc gcacgacatc ctgcatttca agggctgcac
      661 ccggcctttg gtcatccgcg gcaacggggc ggtgctcaaa tgcgcgcccg gcctgcgcta
      721 cgggaccttc gaccgggcgg gccggccgac ccggcacagg ctgcccttct acacgcccgg
      781 cgagctcgcg acgccctacc aatacatgat caagatcgaa tcgtgccgcg gcccgatcgc
      841 tctgtcggac atcgagctcg acggcggcct ggccggcctg cgcatcggcg gcgaatatgg
      901 cgaccacggc cgccagatcc ccgcgaccgg gctcgggctg ttcaacaatt cgggttcgga
      961 gacgatcgcc aatgtccaca cgcaccacca cggccaggac ggcctgatca tcgacgggat
     1021 cgcccggccg gttccgggcg ccgtccggca ggtcaccgcg ctgcgctccg aatataacgg
     1081 ccgccagggc tgcagcatcg tcggcgggcg cggctatgtg tttagcgact gcaagttcaa
     1141 ccacacgggc caggccggca tccagagcgc gccggccgcg ggcgtcgaca tcgaggccga
     1201 gggcgacaag atcaaccgcg acttccgctt cgtgaactgc gagttcgtca acaacggcgg
     1261 cgccgggctg ctcgccgaca gcggcgattc ggagggggtg gtcgccgagc agtgccgctt
     1321 cgtcgggtcg agcagctggg cggcgtggcc gaggaagccg cgcatgcggt tcaccaactg
     1381 caccttcgtc ggccagatcg tgcaatgcca cggcgacccc gaccagtccc gggcggcgca
     1441 gttccactcg tgccgcttct atgacgaccc gaagctgtcg ccgaccggca aattatgggc
     1501 ttcgggcggc gccaacgcgg tcgccgacct cggcgccggc ggcgagaacg tgctgttcag
     1561 caaatgcatc ttcgagctca cccacaggct ggtgttgccg tggagctggc acgcgatcta
     1621 ttccgactgc cggatgcggc agaaatcgcc gcagatcgct tatcccaagg gcacctatct
     1681 cggctcgagc acgatttggg gtaaggtcga catgtatgga accaaggtcc ggggccgcct
     1741 cgttgtgaac ggggtccccc tgagagggta agccggaata tgtgcgggat cgccggaatc
     1801 attgcctcat cgcgtctcga ggagcgcgac gtccggcggc tgatcgaccc gatcacgcac
     1861 cgcggtccgg acgacgaggg cgtgtggatc gatgccgaag ccggcgtcgg cttcggccac
     1921 cggcgcctcg cgatcctcga cctgtcgccg gccgggcacc agccgatgac gtccgcggac
     1981 ggccgctacg tgatcaccta caatgccgaa atctacaatc atgccgagct tcgcgccgag
     2041 ctcgaggcgg cgggcgccgc cccgcccggc ggctggcgcg gccattcgga caccgagacc
     2101 ctgctcgagt gcgtgtccgc ctgggggctg gccgaaacgc tgccccggat cgtcggcatg
     2161 ttcgccttcg ccctgtggga ccgccaggag cgccagctcc atctggtccg cgaccgcttc
     2221 ggcgagaagc cgctctacta cggctgggcg ggcagggact tcttgttcgc gtccgaattg
     2281 aaggccatcc gggcgcaccc gcgcttcgac ggctccatcg accggcgggc gctcaagctg
     2341 ttcgccgcgc gcacctacat cccggcgccg ctgtcgatct accggcggct gttcaagctc
     2401 gagcccggct gcgtgctcac cgtcacgccc gaagcggcga agaccccgct cgacgccccg
     2461 ccgcaggtcg acgggccggc cggcgcaatc cggatccgcc gctactggtc gtaccgcgac
     2521 gtcatgcgcc gcgggctcga gcagccgatc ggcgatgaag gcgaagcgct cgaccggctc
     2581 gagcaggtgc tcgcgacggc gatccggggc cagtcggtcg ccgacgtccc ggtcggcgcc
     2641 ttcctgtcgg gcgggatcga cagctcggcc atcgtcgcgc tctaccagaa atactcctcg
     2701 gtgccggtcc gcaccttcac catcggcttc gaggaagcgg ggttcaacga ggccgaggac
     2761 gccaagaagg tcgccgcgca cctcggcacc gtccactccg agcgctacgt cacggtcaag
     2821 gaagcccgcg acgtcatccc actgctgcca gcgatgtacg acgagccgtt cgccgattcc
     2881 tcgcagatcc cgaccttcct cgtcagccga ttcgcgcgcg aacaggtgac cgtggcgctg
     2941 accggcgacg gcggcgacga attgttcgcc ggctacaacc gccatttcgc cgccccgcag
     3001 ctgtggcagc agctgcgctg gctgcccagg ccgctgcgct cggcgatcgg ctcgccattg
     3061 agccggcttc cgtcgcaatt ctggaacgga gcggtcggct tgctgtcggg ccgccgccgg
     3121 ccgcacctcg gcggcaagat ccagaaaggg ctccgggtcg cgggctcggt gcgcaatttc
     3181 gacgagctgt actcaagctt cctcgacgaa tggagcttcg aggattcgcc ggtccggggc
     3241 ggcgacggcg ccgggcccgg atgggacatg gacatcgccg accaggcccc ggacacgctg
     3301 cggatgatgt attgcgacgc ggtttcgtac ctgcccgacg acattttgtg caaggtcgac
     3361 cgggcgtcga tggcggtcag cctcgagacc cgcgtgccct atctcgacca tcgcgttgcc
     3421 gagctcgccg cgcgcatccc gctggaactg aaagtgcgcg gaggaagcgg caagcacatc
     3481 cttcgcgagc tgctctaccg cgaggtgccg cgcgaactgt tcgagcgccc caaggcgggc
     3541 ttcgcgatcc cggtcggcga atggatcaag ggctcgctgc ggccgtgggc cgaggatttg
     3601 ctcgacccgt cgcggatggc cggggagggc tggttcgacc cggcgatcgt ccagcgccgc
     3661 tggaaggagc acctcggcgg ccgccgcgat tcgaccccgg cattgtgggc ggtgctgatg
     3721 ttccagtcgt ggctgcgcga gcagcgccag ccgctgtccg ccgccgctta gtcggtcccg
     3781 gtccggctct cgggctggcc gaacacttcg gcaaacgtgc cgagctcggc gatccggccg
     3841 ttgtcgagcc gggcgaccag gtcgcaatgc tcgacggtcg agcgccggtg ggcgatgatg
//
LOCUS       DASTZO010000328         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_387421,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000328 DASTZO010000000
VERSION     DASTZO010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_387421"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..725
                     /locus_tag="VFU20_09270"
     CDS             <1..725
                     /locus_tag="VFU20_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5286675.1"
                     /translation="AAGLASHGGRIVALSRRGQIPRAHAEFAAAPVEQHDLPGGGLLA
                     LWRWLRRRSGEVGWRAAVDALRPHSHQLWQGLGAEQQRRFLRHARPWWDVHRHRIAPE
                     VASRIVALVADGRLEIVAGRIAAVRPADGGLEVEIRRRGTPGSETRRFAYVFNCTGPL
                     GSIGRTRDPLLRLLLDDGWVRPDHLGIALEVDERSRAAGADRLWALGPLTKGCYWEIV
                     AVPDIREQAAAVADDIARELGS"
     gene            722..1357
                     /gene="folE"
                     /locus_tag="VFU20_09275"
     CDS             722..1357
                     /gene="folE"
                     /locus_tag="VFU20_09275"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU5286676.1"
                     /translation="MTRSTGPGPGCEPEDGDLVQSPPAVEVPEDVAEAVRTLIRWAGD
                     DPDREGLLDTPQRVARAWREYARGYEEDPAVHLARTFDEVGGYDEIVLLKDIPFQSHC
                     EHHLAPIIGKASIAYLPRDKVVGISKLARVLHGYARRLQVQERLTAEVADCICEHLQP
                     KGVAVVIEASHACMSARGVNTPGVMMTTSRMMGIFRSDERSRREVLALMGY"
     gene            1374..1901
                     /locus_tag="VFU20_09280"
     CDS             1374..1901
                     /locus_tag="VFU20_09280"
                     /inference="COORDINATES: protein motif:HMM:NF025852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5286677.1"
                     /translation="MTQGISKMAAMAAMACLVAGCEVRESAQPAEPATNAANPAAAAA
                     PSADASKVPLAKAAADPGLQWGPCPEGMPEGCGIAVLHGDPAKPNADIFLRVPGGSAI
                     PPHSHSSAERMILVGGELEAKYQGADALTLRPGHYAYGPAKLPHRAQCRSSEPCILFI
                     AFEGPVDLLPFEGAI"
     gene            1996..2589
                     /locus_tag="VFU20_09285"
     CDS             1996..2589
                     /locus_tag="VFU20_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019517765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286678.1"
                     /translation="MAKGKPQKTSPGRKQKLKVFRTPVGFDDAYVAAPSRKAALAAWG
                     AGTDLFAAGVAELVTDPELTKEALERPGEVVRKSRGSADEQVAALGAGKAKKTEAGSR
                     ISSGMTKTGEGRGAKKEGGRGRRPSPAAVDRAEAAIGRAEARHRAALAQLKAQAEAID
                     KRRRELERTRRAERDKLEAALDEARERYRSAMEEWAR"
     gene            2682..>3257
                     /locus_tag="VFU20_09290"
     CDS             2682..>3257
                     /locus_tag="VFU20_09290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU5286679.1"
                     /translation="MQRSRSKAVRLAGSHLITTCATMAAILLFVGLGSQILPFALAGI
                     ELPNASGGSLRLAFLLNIAIILFGWRRSKDLKVALDAYERAERAAHRNANTDHTTGLA
                     NRRELLRSLADLLETRTPGVLLLLDLDHFKRVNDLHGHLVGDQLLKAAADSIRDAAPA
                     AACCARIGGDEFAILLGDSSPAEAEALAERLL"
BASE COUNT          502 a         1086 c         1241 g          428 t
ORIGIN      
        1 acgccgccgg cctcgccagc cacgggggcc ggatcgtcgc cctgtcgagg cgcggccaga
       61 tcccgcgggc ccacgccgag ttcgccgccg ctccggtcga gcagcacgac ctgccgggcg
      121 gcgggctgct ggccctgtgg cgctggctgc ggcggcgcag cggcgaggtc ggctggcgcg
      181 ccgcggtcga cgcgctccgg ccgcatagcc atcagctgtg gcaggggctc ggcgccgagc
      241 agcagcgccg cttcctcagg cacgcgcggc cgtggtggga cgtccaccgg caccgcatcg
      301 cacccgaggt ggcgagccgg atcgtcgccc tggtcgccga cggcaggctg gagatcgtcg
      361 ccggccggat cgccgcggtc cgcccggccg acggcgggct cgaggtggag atccgccggc
      421 gcggcacgcc cggcagcgag acgcggcgct tcgcttacgt gttcaactgc accgggccgc
      481 tcggctcgat cggccgcacc cgcgacccgc tgctgcgcct gctgctcgac gacggctggg
      541 tgcgccccga ccatctcggg atcgcgctcg aggtcgatga gcgctcgcgc gccgcgggag
      601 ccgaccggct gtgggcgctg gggccgctga ccaagggctg ttactgggaa atcgtcgcgg
      661 ttccggacat aagggagcaa gcggcggcgg tggccgacga catcgcaagg gagcttggat
      721 catgacccgt tcgactggcc cgggaccggg ctgcgagccc gaggacggcg atttggtgca
      781 atcgccgcct gcggtcgaag tgcccgagga cgtcgccgag gcggtccgca ccctgatccg
      841 ctgggccggc gacgaccccg accgcgaggg cctgctcgac actccgcagc gggtcgcgcg
      901 cgcgtggcgc gaatatgccc gcggctacga ggaggacccg gcggtgcacc tggcgcgcac
      961 cttcgacgag gtcggcggct acgacgagat cgtgctgttg aaggacattc cgttccagtc
     1021 gcactgcgag caccatctgg cgccgatcat cggcaaggcg tcgatcgcct atctgccgcg
     1081 cgacaaggtg gtcgggatca gcaagctcgc ccgggtgctg cacggctatg cgcggcggct
     1141 gcaggtccag gagcggctga ccgccgaggt cgccgactgc atctgcgagc accttcagcc
     1201 caagggagtc gcggtggtca tcgaggccag ccacgcgtgc atgagcgcgc gcggcgtcaa
     1261 cacgccgggc gtgatgatga ccaccagccg gatgatgggg attttccgca gcgacgagcg
     1321 gagccggcgg gaggtgctgg cgctgatggg gtactaaaca gggagaggaa gtgatgactc
     1381 agggaatctc gaagatggcg gcgatggcgg cgatggcttg cttggtcgct gggtgcgagg
     1441 tgcgcgagag cgcgcagccg gccgagcccg ccaccaatgc cgccaacccg gccgcggccg
     1501 ccgctccgtc tgccgacgcg tcgaaggttc cgctggcgaa ggcggcggcg gacccggggc
     1561 tgcaatgggg gccgtgcccg gaagggatgc ccgagggctg cgggatcgcg gtgctgcatg
     1621 gcgacccggc caagcccaac gcggacattt tcctccgagt gcccggcggc tcggcgatac
     1681 cgcctcattc gcacagctcg gccgagcgga tgatcctggt cggcggcgag ctggaagcga
     1741 aataccaggg cgccgacgcg ctcacccttc gccccgggca ttacgcctac ggcccggcga
     1801 agctgccgca ccgcgcccag tgccgctcga gcgagccgtg catcctgttc atcgccttcg
     1861 aggggccggt cgacctcctg ccgttcgagg gggcgatctg atcggctttg gctcgaaaac
     1921 caggtcgcgc cgatgccgat tgaattgctc cggcgcgggg tgaacccgtg cttcgcaatg
     1981 agtcgttgag cgggaatggc caaaggaaag ccgcaaaaaa cctcacccgg ccgcaagcag
     2041 aagctcaagg tgttccggac gccggtcggg ttcgacgacg cttatgtcgc ggcgccgagc
     2101 cggaaggcgg cgctggcggc ctggggggcg gggaccgatt tgttcgccgc cggggtggcg
     2161 gagctggtca ccgatcccga gctgacgaaa gaggcgctgg agcggcccgg cgaggtggtg
     2221 cgcaagtcgc gcggcagcgc ggacgagcag gtcgcggccc tgggggccgg caaggcaaaa
     2281 aagacagaag cgggatcccg aatctcgtcc gggatgacga agacggggga agggagagga
     2341 gcgaagaagg aaggcgggag agggcggcgg ccgagcccgg cggcggtgga ccgggccgag
     2401 gcggcaatcg ggcgggccga ggcccggcac cgcgcggcgc tggcgcaatt gaaggcgcag
     2461 gccgaggcga tcgacaagcg gcggcgcgag ctcgagcgca cgcggcgggc cgagcgcgac
     2521 aagctcgagg cggcgctcga cgaggcgcgc gagcgctatc gctcggcgat ggaggaatgg
     2581 gcgcgatagg ccgggcgccc gggaattcgc cgcctcggta ggggtatttt cacatctttg
     2641 cgcgaagagg gggggtggac gaccagcgcg acggaagatc agtgcaacgg tcaagaagca
     2701 aggcggtccg gcttgccgga agccacctca tcacgacctg cgcgacgatg gcggcgatcc
     2761 tgctgttcgt cgggctgggc agccagatcc tgccgttcgc gctcgccggc atcgagttgc
     2821 cgaacgcgtc ggggggcagt ttgcgcctgg cgttcctgct caacatcgcc atcatcctgt
     2881 tcggctggcg gcgatccaag gacctcaagg tggcgctcga cgcctatgag cgggccgagc
     2941 gcgcggccca ccgcaacgcc aacaccgacc acaccaccgg gctggccaac cggcgcgagc
     3001 tgctgcgctc gctcgccgac ctgctcgaga cgcgcacccc gggggtgctg ctgctgctcg
     3061 acctcgacca tttcaagcgg gtcaacgacc ttcatggcca tttggtcggc gaccagctgc
     3121 tcaaggccgc ggccgactcg atccgcgacg ccgcgccggc ggcggcgtgc tgcgcccgga
     3181 tcggcggcga cgagttcgcc atcctgctcg gcgattcgag ccccgcagag gccgaagcgc
     3241 tcgccgagcg gctgctc
//
LOCUS       DASTZO010000329         6655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_987878,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000329 DASTZO010000000
VERSION     DASTZO010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 6655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6655
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987878"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VFU20_09295"
     CDS             complement(<1..430)
                     /locus_tag="VFU20_09295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004210075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEU5286680.1"
                     /translation="MLKTSEAAAGGPLKELTIRGIILGAILTVVFTAANVYLGLRAGI
                     TFATSIPAAVISMAVLKAFRDSTIQENNIVQTIASSAGTLSAIVFVLPGLVMVGWWTG
                     FPYWLSVAVIGIGGILGVMYSVPLRRAMVTGSDLPYPEGVA"
     gene            complement(451..873)
                     /locus_tag="VFU20_09300"
     CDS             complement(451..873)
                     /locus_tag="VFU20_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5286681.1"
                     /translation="MVDEPADKGAEGTRELIGAVERQWRDALCAKDMDLLRSLIHPRF
                     VLIGTRSSGPFTMNRDEWLDAIQRREVISIEMEIEDALVLDQVMVGTVQAKWCVRYLG
                     REVEDRVLLTDVWVFDAGHWRVVRRHSSPDPGSHRIKS"
     gene            complement(863..2473)
                     /locus_tag="VFU20_09305"
     CDS             complement(863..2473)
                     /locus_tag="VFU20_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015458674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HEU5286682.1"
                     /translation="MSDSAIFIGSGAGGTDPQQLELSRANRHGLIAGATGTGKTVTLQ
                     GLIEGFCAAGVPTFVADVKGDLAGLAMPGSATGKAHEIFSARAAEIGFADWRYDACPV
                     QLWDLFGEQGHPIRTTVSEMGPLLLARLMNLNEVQEGVLTIAFHVADKDGLLLLDLDD
                     LQTMLVDVGERAEELTLTYGNVSKPSVGAIQRSLLQLRSQSGDRFFGEPAVELEDFLR
                     LDENGRGVVNILAADRLMASPRLYSTFLLWLLSELFEQLPEIGDQPKPKLCFFFDEAH
                     LLFDDAPPALLEKVEQVVRLIRSKGVGVYFITQNPIDIPDQIAGQLGNRVQHALRAFT
                     PRDQAAVKSAAETFRQNPEIDVATAITELKVGEALVSLLQPDGAPSPVQRTLVKPPAS
                     RVGPLTPEERKVLIATDEIGARYDVPVDRESAQEMLASKATEAAAAAAAAKAETDAER
                     AAADKAKADAVAARDALKLQRERERLEAQRRREEQRAADRAAREAARPTMTDKMMQSA
                     GRAVASSVGRQIGNQLLRGILGGLLRGR"
     gene            complement(2561..4081)
                     /locus_tag="VFU20_09310"
     CDS             complement(2561..4081)
                     /locus_tag="VFU20_09310"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019830995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HEU5286683.1"
                     /translation="MRYAYGVAMALLLGGTAFSIASGPVGAQVAQNAPTAMAPRPGAP
                     LSFAELAARLQPAVVNISTKQRVPVRTQTDPFEEFFRRFGAPGAQPPGGVTPRTRETG
                     SLGSGFIISPDGYVVTNNHLITGATGTGTVDTVTVTLTNRKEYTARIVGRDATSDLAL
                     LKIDGSNLPYVNWGDSTRSRVGDWVIAIGNPYGLGGTVTAGIISALHRGITGVGAYDR
                     YIQTDASINMGNSGGPMFDLNGNVIGINSALISPTGASVGIGLAIPAELAKPVIDSLR
                     RGQRPQRGYLGISLQPLDENIAPTLGLPKDSGEIVRWVQPGEAAARAGIQAGDVVVRV
                     NGQPVTPDQTASYLIANTQVGARIPIDIIREGRQMRIQATVGQRPTEEQLAALAGGGE
                     GEAAIIGGGPSVAPRQVLGLSLQPLTPELARGARLPPTARGVIVVAVDPASDAAEEGL
                     QRGDLIMSVNRQPVTTPAQVTAAVEAARRAGRTSVLLLVKRGPGPEAFVGIELGAR"
     gene            complement(4197..5057)
                     /locus_tag="VFU20_09315"
     CDS             complement(4197..5057)
                     /locus_tag="VFU20_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017182619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HEU5286684.1"
                     /translation="MALVRRRPLLTGLVGVFLLFTVLGSFPIVPETKQAVVVRFGKPV
                     RILNRFQPGEPIGGPGAGLSWRIPFAENIQWIDKRVLDVDMDRQQVLSTDQLRLEVDA
                     FARYRITDPLRMYIRARTEERVSEALRPILGSELRNELGKRPFAALLSPERQGVMENV
                     RRGLDRVARQYGAQIIDVRIKKADLPDGTPLQSAYDRMRTARLQEARSIRAQGAKQAQ
                     IIRAEADAEAAKTYAASFGKDPGFYDFYRAMQSYQTTFIGDGAERPSPTTIILSPQND
                     YLKEFTGRAR"
     gene            complement(5057..6139)
                     /gene="hflK"
                     /locus_tag="VFU20_09320"
     CDS             complement(5057..6139)
                     /gene="hflK"
                     /locus_tag="VFU20_09320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010408394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HEU5286685.1"
                     /translation="MSILSGLGARARGLFTDNKGPWGPKGGNGDEPPPPEGGGGPWGE
                     PPRRGRRTTGPQPGNVASLEDFLRRSRARFGEGGGFPGRPDRSIVVWAVLALVILWLL
                     FTTMHRIAPEERGVVTRFGRYSHTLGPGISLTLPAPIDRVRKLDVENIRNIDLGSTGT
                     ETLMLTGDQNIVDIAYSVRWNIRDPELYLFELAQPDETIRQVGESAMRAVVSRYSLND
                     TIGEGRGDIETRVQDVMQTVLNNYRSGVRIQGVAIKQADPPQAVNDAFKEVSAAQQEA
                     QSYINQARAYALQLTAKAQGEATAFDKVYEQYRLSPDVTRRRMYYETMERVLANVDKT
                     IVEPSGVTPYLPLPQVQKSAPREAGQ"
     gene            6165..>6655
                     /locus_tag="VFU20_09325"
     CDS             6165..>6655
                     /locus_tag="VFU20_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016744809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HEU5286686.1"
                     /translation="MKNSGKRRRSSYVAAMGDTKDPLSSLDALPDAGRIRSRKLVGDI
                     ASLVVDATGLDEQGRDALERDLRSAALDVPGVKQARVALTARQPGRKLIAIGSGKGGV
                     GKSTLAANLAIALARSGKKVGLIDADIYGPSQPRLLGSDAKPTAEGDRLIPVAAHGLH
                     FLSL"
BASE COUNT          980 a         2255 c         2372 g         1048 t
ORIGIN      
        1 ctgcaacgcc ttccgggtag ggaagatcgg agccggtcac catcgcccgc ctgagcggga
       61 ccgaatacat gacgccgagg atgccgccga tgccgatcac ggcgaccgac agccagtagg
      121 gaaagccggt ccaccagccg accatcacca ggccgggaag gacgaacacg atcgccgaca
      181 aggtcccggc cgaggaggcg atcgtctgga cgatgttgtt ttcctggatg gtcgagtcgc
      241 ggaacgcctt caggaccgcc atcgagatca ccgcggccgg gatcgaggtg gcgaaggtga
      301 ttccggcgcg aagcccgagg tagacgttgg cggcggtgaa cacgacggtg aggatggccc
      361 cgaggataat gccccggatc gtcagctcct tgagagggcc gcccgccgcc gcttcgctgg
      421 tcttcagcat tgccgttccc ctcccgaaag tcaggacttg atccggtgcg agcccgggtc
      481 gggactcgaa tggcgccgaa cgacccgcca gtggcccgcg tcgaacaccc agacgtcggt
      541 caggagcacg cgatcctcga cctcgcggcc gagatagcgg acgcaccatt tcgcctggac
      601 ggtgccgacc atcacctggt cgaggaccag cgcgtcctcg atctccatct cgatgctgat
      661 gacctcgcgc cgctggatcg cgtcgagcca ttcgtcgcgg ttcatcgtga acggcccgct
      721 cgagcgggtg ccgatgagga cgaagcgcgg gtggatcagc gagcgcagca ggtccatgtc
      781 cttggcgcac agcgcgtcgc gccactggcg ctcgaccgcc ccgatcagct cgcgggtccc
      841 ctcggcgccc ttgtcggccg gttcatcgac cacgcagcag ccctccgagg atcccgcgca
      901 gcagctggtt gccgatctgc cgtccgaccg agctggccac cgcgcgcccg gccgactgca
      961 tcatcttgtc ggtcatcgtc ggccgggcgg cctcgcgcgc ggcgcggtcg gcggcgcgtt
     1021 gctcctcgcg ccggcgctgc gcctcgagcc gttcgcgctc gcgctgcagc ttcagcgcgt
     1081 cgcgggccgc gacggcatcc gccttggcct tgtccgccgc ggcgcgctcg gcgtcggtct
     1141 cggccttggc ggcggccgcg gcggcagcgg cctcggtcgc tttggaggcg agcatctcct
     1201 gggccgactc gcggtcgacc ggcacatcgt agcgcgcgcc gatttcgtcg gtggcgatca
     1261 gcaccttgcg ctcctcgggc gtcagcggcc cgactcgcga ggccggcggc ttgaccagcg
     1321 tccgctggac cggcgacggc gctccgtccg gctggagcag cgacaccaga gcctcgccga
     1381 ccttgagctc ggtgattgcg gtcgcgacgt cgatctccgg gttctgcctg aaggtctccg
     1441 cggcggattt caccgccgcc tggtcgcggg gagtgaaggc gcgcagcgcg tgctggacgc
     1501 ggttgccgag ctggccggcg atctggtcgg ggatgtcgat cgggttctgg gtgatgaaat
     1561 agacgccgac gcccttcgaa cggatcagcc ggaccacctg ctcgaccttc tcgagcaggg
     1621 ccggcggggc gtcgtcgaac agcaaatggg cttcgtcgaa gaagaagcac agtttgggct
     1681 tgggctggtc gccgatctcg ggaagctgct cgaacagctc ggacagcagc cacagaagga
     1741 aggtcgagta gagcctcggg gacgccatca gccggtcggc ggcgaggatg ttgacgaccc
     1801 cgcggccatt ctcgtcgagc ctgaggaaat cctcgagctc gaccgccggc tcgccgaaga
     1861 aacggtcgcc gctttggctt cggagctgca gcagcgagcg ctggatcgcg ccgaccgagg
     1921 gcttggacac gttgccgtag gtcagggtca gctcctcggc gcgctcgccg acgtcgacca
     1981 gcatggtctg caggtcgtcg aggtcgagga gcagcaggcc gtccttgtcg gcgacgtgga
     2041 aggcgatggt gaggacgccc tcctggacct cgttcaaatt catcagccgg gcgagcagca
     2101 gcggccccat ttcgctgacc gtggtgcgga tcgggtggcc ctgctcgccg aataggtccc
     2161 acagctgcac cgggcaggca tcgtagcgcc agtcggcaaa gccgatctcg gcggcccggg
     2221 cgctgaagat ctcgtgggcc ttgccggtcg cggagcccgg catcgcgagg ccggcgagat
     2281 cgcccttgac gtcggcgacg aaggtcggga ccccggccgc gcaaaagcct tcgatcaggc
     2341 cctgaagggt caccgtcttg ccggtgccgg tggcgccggc gatcaggccg tggcggttgg
     2401 cgcgggacag ttccagctgc tgcggatcag tgcccccggc gccgctgccg atgaagatcg
     2461 cggaatcgct caagccgggc tctctcctcc gcgcgtccct ttgccgacgc ggagggtaag
     2521 cgcaagtagg cgcggcagcc ggccgggccg aaggacgagc ctagcgggct ccgagctcga
     2581 tcccgacgaa ggcctcgggg cccggtccgc gcttgaccag cagcagcacg ctggtgcggc
     2641 cggcccggcg cgcggcctcg acggcggccg tgacttgcgc cggagtagtc accggctggc
     2701 ggttgaccga catgatgagg tcgccgcgct ggaggccttc ctcggcggcg tcgctggcgg
     2761 ggtccaccgc gacgacgatc actccgcgag cggtcggcgg cagcctcgcg cctcgcgcaa
     2821 gctccggagt cagcggctgc agcgacagcc cgagcacctg gcgcggggcc accgacggcc
     2881 cgccgccgat gatcgcggcc tcgccttcgc cgccgccggc cagtgccgcc aattgctcct
     2941 cggtcggccg ctggccgacc gtcgcctgga tccgcatctg gcggccctcg cgaatgatgt
     3001 cgatcgggat gcgcgcgccg acctgggtgt tggcgatcag ataggatgcg gtctggtcgg
     3061 gggtgaccgg ctggccgttg acccggacga cgacgtcgcc ggcctggatc ccggcccgcg
     3121 ccgcggcctc gcccggctgg acccagcgga caatctcgcc gctgtccttg ggaaggccga
     3181 gcgtcggggc gatattctcg tcgagcggct gcaggctgat gccgaggtag ccgcgctgcg
     3241 gacgctggcc gcggcgaagc gaatcgatca ccggcttggc aagctcggca gggatggcga
     3301 ggccgatccc gaccgacgcc ccggtcggcg agatcagcgc cgagttgatg cctatgacgt
     3361 tgccgttcaa atcgaacatc gggccgcccg aattgcccat gttgatcgac gcgtcggtct
     3421 ggatgtagcg gtcgtaggcg ccgacgcccg tgatgccgcg gtgcagcgcc gagatgatcc
     3481 ccgcggtcac cgtcccgccg agcccgtagg ggttgccgat cgcgatcacc cagtcgccga
     3541 cccggctgcg ggtgctgtcg ccccagttca cataaggcag gttggacccg tcgatcttga
     3601 gcaaagcgag gtcggaggtc gcgtcgcggc cgacgatccg ggcggtatat tccttgcggt
     3661 tggtcagtgt gacggtgacg gtgtcgaccg taccggtgcc ggtggcgccg gtgatcagat
     3721 ggttgttggt gacgacatag ccgtcgggcg aaatgatgaa gcccgagccg agcgagccgg
     3781 tctcgcgggt gcgcggagtc actcccccgg gcggctgcgc gccgggcgct ccgaagcgcc
     3841 ggaagaattc ttcgaacggg tcggtctggg ttcggaccgg gacgcgctgc ttggtggaga
     3901 tgttgaccac cgccggctgc agccgcgcgg cgagctcggc gaatgacaag ggcgcgccgg
     3961 ggcgcggcgc catcgccgtc ggagcgttct gggcgacctg cgcgccgacc gggccgctcg
     4021 cgatcgagaa agcggttcca cccagcagca gggccatcgc gaccccatag gcatagcgca
     4081 cttttccgga ctccttcgaa ctcatcgata ctccatgact tggtctgcgg aagcaatgca
     4141 atgcgtcgtc gtgccctaat ccgaacgggc cttaatccgc attgaacgaa cgtttgtcag
     4201 cgcgcgcggc cggtgaattc cttcaaatag tcgttctgcg gcgacaggat gatggtcgtc
     4261 ggcgatggcc gctcggcacc gtcgccgatg aaggtagtct ggtaggactg catcgcgcgg
     4321 tagaaatcgt agaagccggg atccttgccg aagctcgccg cgtaggtctt ggcggcctcg
     4381 gcgtcggcct cggcgcggat gatctgcgcc tgcttggcgc cctgggcgcg gatcgagcgc
     4441 gcttcctgga gcctggcggt gcgcatccgg tcgtaggccg actgcagcgg agtgccgtcg
     4501 ggcaggtccg ccttcttgat ccggacgtcg atgatctggg cgccatattg ccgcgccacc
     4561 cggtcgaggc cgcggcggac gttctccatc accccctgcc gctcgggcga cagcagcgcc
     4621 gcgaacggtc gcttgccgag ctcgttgcgc agctccgagc cgaggatcgg gcggagcgcc
     4681 tcgctgaccc gctcctcggt ccgggcgcgg atatacatcc gcagcggatc ggtgatccgg
     4741 tagcgggcga acgcatcgac ctcgagccgc agctggtcgg tcgacagcac ctgctggcgg
     4801 tccatgtcga catcgagcac gcgcttgtcg atccactgga tgttctcggc gaacgggatt
     4861 cgccacgaca ggccggcgcc cggcccgccg atcggctcgc ccggctggaa gcggttcagg
     4921 atccgaaccg gcttgccgaa gcgcacgacc accgcctgct tggtctcggg gacgatcggg
     4981 aagctgccga gcacggtgaa cagcaggaag accccgacga ggccggtgag cagcggccgg
     5041 cggcgaacga gcgccatcat tggcccgcct cccggggcgc cgacttctgc acctgcggca
     5101 gcggcaggta cggagtgacc ccgctcggct cgacgatcgt cttgtcgacg ttggcgagca
     5161 cccgctccat cgtctcgtaa tacatgcggc gccgggtgac gtcgggcgac agccggtatt
     5221 gctcgtagac cttgtcgaac gcggtcgcct cgccctgcgc cttggcggtc agctggagcg
     5281 cgtaggcgcg cgcctggttg atgtaggact gcgcctcctg ctgcgcggcc gagacttcct
     5341 tgaacgcgtc gttgaccgcc tgcggcgggt cggcctgctt gatcgcgacg ccctggatcc
     5401 ggacccccga gcgataattg ttgagcacgg tctgcatcac gtcctggacc cgggtctcga
     5461 tgtcgccgcg gccctcgccg atggtgtcgt tgaggctgta gcggctgacc accgcgcgca
     5521 tcgcgctctc gccgacctgg cggatggtct cgtcgggctg ggcgagctcg aacaggtaca
     5581 gctcggggtc gcggatgttc cagcgcaccg aataagcgat gtcgacgatg ttctggtcgc
     5641 cggtcagcat cagcgtctcg gtgccggtcg agccgaggtc gatgttgcgg atgttctcga
     5701 cgtcgagctt gcgcacccgg tcgatcggcg ccggcagcgt caggctgatg ccggggccga
     5761 gcgtatggct gtagcggccg aagcgggtga cgactccgcg ctcctcgggc gcgatccggt
     5821 gcatggtcgt gaacagcagc cagaggatga cgagggcgag caccgcccag acgacgatcg
     5881 agcggtcggg ccggccgggg aagccgccgc cttcgccgaa gcgggcgcgg ctgcgcctta
     5941 ggaaatcctc gagcgaggcg acgttgccgg gctgaggacc ggtcgtccgg cggccgcgcc
     6001 tgggcggctc gccccacggc ccgccgccgc cctcgggcgg gggcggctcg tcgccgttgc
     6061 cgccctttgg accccacgga cccttgttgt cggtgaacag gccgcgagcg cgtgcgccca
     6121 gcccggataa gatgctcatg gccccactat aggaagcctc actcatgaaa aacagtggca
     6181 agcgtcggcg gagttcctat gtcgccgcga tgggcgacac caaggatccg ctgagcagcc
     6241 tcgacgcgct gcccgatgcc ggccggatcc ggtcgcgcaa gctggtcggc gacatcgcct
     6301 cgctggtcgt cgatgcgacc ggcctcgacg aacagggccg cgacgcgctc gagcgcgatc
     6361 tccgctcggc cgcgctcgac gtgcccggag tcaagcaggc ccgggtcgcg ctcaccgccc
     6421 gccagccggg ccgcaagctg atcgcgatcg gcagcggcaa gggaggcgtc ggcaagtcga
     6481 cgctcgcggc gaacctcgcc atcgccctcg cccgctccgg caagaaggtc ggactgatcg
     6541 acgccgacat ctacgggccg tcgcagcccc ggctgctcgg cagcgacgcc aaaccgaccg
     6601 ccgagggcga ccggctgatc ccggtcgccg ctcacgggct gcatttcctg tcgct
//
LOCUS       DASTZO010000330         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_1301058,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000330 DASTZO010000000
VERSION     DASTZO010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301058"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VFU20_09330"
     CDS             complement(<1..1302)
                     /locus_tag="VFU20_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286687.1"
                     /translation="MQFPDMTKLARLLLVLALAIAAGAVSAQERPRPAQAPQQAEQSQ
                     TDQRTSGSQDGVLRLLPADSVTEHSVDIPAGSLRYTATAGTLSLYDQTGDRSAAIFYT
                     AYVAKNADAANRPITFAFNGGPGAASAFLTLGLVGPRIAELVDHDPSATQLRDNPHTW
                     LGFTDLVMVDPVGTGWSRAAKPDGAGAFYGVRRDAEALAKFVALYLAKNGRNASPKYL
                     LGESYGGYRAVKVAHALQRQQGIAVSGIVMVSPLMEAALMFSGNRFALGAALQLPSLA
                     AAELERQGKFTPEAQAEAERFAMNEYLVTLAGAPPKDDRAREFYAKLAEVSGLPEAVV
                     TRSRGFIRDAYVKHLRSAERKIVSHYDATYAVADPYPDRETSRGPDPLLDGLTRAYGS
                     AFANYARDELGYKSDMTYVLLASDISGKWDWEGGGRSQASIS"
     gene            1474..>2005
                     /locus_tag="VFU20_09335"
     CDS             1474..>2005
                     /locus_tag="VFU20_09335"
                     /inference="COORDINATES: protein motif:HMM:NF014564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HEU5286688.1"
                     /translation="MPFPKASRALERFTVLDLTRVRSGPTCVRQLADWGANVIKIELP
                     PDPSAGEALGGPREGSDFANLHRNKRSMTLNLKAPEAVAALKRMVKKADVVVENFRPD
                     VKRRLGIAYKTLAKINPKLVYASISGFGQTGPYANRPGFDQIAQGMGGLMSITGLPGQ
                     GPVRVGIPIADLTAGLF"
BASE COUNT          318 a          689 c          691 g          307 t
ORIGIN      
        1 cgaaatgctg gcctggctgc ggccgccgcc ttcccagtcc cacttgccgc tgatgtccga
       61 ggcaagcagc acgtaggtca tgtcggactt gtagccaagc tcgtcgcgcg cgtagttcgc
      121 aaacgcgctg ccgtaggcgc gggtcaggcc gtcgagcagc gggtcgggac cccgcgaggt
      181 ctcccgatcc ggataagggt ctgcgaccgc ataggtcgca tcgtagtgac tgacgatctt
      241 gcgttcggcg gaccgcaggt gcttgacgta ggcgtcgcgg atgaagccgc gcgacctggt
      301 cacgaccgcc tcggggagcc cgctcacctc ggcaagcttg gcgtagaact cgcgcgcccg
      361 gtcgtccttg ggcggcgcgc cggcaagcgt caccagatat tcgttcatcg cgaaccgctc
      421 ggcctcggcc tgcgcttcgg gcgtgaactt gccctggcgt tccagctcgg ccgccgccag
      481 cgacggcaac tgcagcgccg caccgagcgc gaagcggttg ccgctgaaca tcagcgccgc
      541 ctccatcaaa ggcgacacca tgacgatgcc cgagaccgcg atgccctgct ggcgctgcag
      601 cgcgtgcgcc accttgacgg cgcgatagcc gccgtagctt tcgcccagga gatacttcgg
      661 cgaggcgttg cggccgttct tcgcgaggta gagcgcgacg aacttggcga gcgcctcggc
      721 gtcgcgccgc acgccgtaga acgcacccgc accgtcgggc ttggcggcgc ggctccagcc
      781 ggtgccgacc ggatcgacca tgacgagatc agtgaagccg agccaggtgt gcggattgtc
      841 gcgcaactgc gtcgccgacg gatcgtgatc gacgagttcg gcaatgcgcg ggccgaccag
      901 gccgagagtc aggaaagcgg acgccgcgcc cggcccgccg ttgaaagcga acgtgatcgg
      961 gcgattggcg gcgtcggcgt tcttggcgac gtaggcggtg tagaagatcg ccgccgagcg
     1021 gtcgccggtc tggtcgtaga gcgagagcgt gccggcggtg gcggtatagc gcagcgagcc
     1081 ggccgggatg tcgacgctgt gctcggtcac cgaatccgcc ggcaacagcc gcagcacgcc
     1141 gtcctgcgag ccggacgtcc gttgatcggt ctggctctgc tcggcctgct gcggggcttg
     1201 cgcgggccgc ggccgctcct gcgcgctcac cgcgccggcc gcgatcgcaa gcgccagcac
     1261 gagcaggagc cgggcaagct tggtcatgtc tggaaattgc atctgggcgt cctggtcgga
     1321 actcttgggc gccccccggc gcgggcacca tagcggaaga agccctcacc gctcaaaccc
     1381 gttcctcggt ggacaaggcc cgggatatcg gggaatgtca gcgtccccgg cgcctgccgg
     1441 gccaacaaga accccccgat cgggttgccg gaaatgccgt ttcccaaggc ttcgcgcgcg
     1501 cttgagcgct ttaccgtcct cgacctgacc cgcgtgcgct ccggcccgac ctgcgtgcgc
     1561 cagctcgccg actggggcgc caacgtgatc aagatcgagt taccgcccga ccccagcgcc
     1621 ggcgaagcgc ttggcggccc gcgcgagggt tccgacttcg ccaacctgca ccgcaacaag
     1681 cgcagcatga cgctgaacct gaaagcgccc gaggcggtcg cggcgctcaa gcgcatggtc
     1741 aagaaggccg acgtcgtggt ggagaacttc cgccccgacg tgaagcgccg gctcggcatc
     1801 gcctacaaga cgctcgccaa gatcaatccg aagctggtct acgccagtat ttcgggcttc
     1861 ggccagaccg ggccttacgc caaccggccg ggcttcgacc agatcgcgca gggcatgggc
     1921 gggctgatgt cgatcaccgg gcttccgggg cagggcccgg tgcgggtcgg cattccgatc
     1981 gccgacctga ccgcaggcct gttct
//
LOCUS       DASTZO010000331         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_74561,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000331 DASTZO010000000
VERSION     DASTZO010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74561"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..123)
                     /locus_tag="VFU20_09340"
     CDS             complement(<1..123)
                     /locus_tag="VFU20_09340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin biosynthesis protein MoeB"
                     /protein_id="HEU5286689.1"
                     /translation="MSLSDAELERYARHIVLREVGGTGQRKLKATSIALVGAGAI"
     gene            complement(120..557)
                     /gene="dut"
                     /locus_tag="VFU20_09345"
     CDS             complement(120..557)
                     /gene="dut"
                     /locus_tag="VFU20_09345"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019831110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEU5286690.1"
                     /translation="MIEIKLYRLPHGDGLPLPKYASEDAAGLDIASAEELVLGPGERH
                     AVATGFAIEIPHGYEVQVRPRSGLAIRNGITCLNTPGTIDSDYRGEVKVILVNLGSEP
                     FAVRRGERIAQLVPAPVLRAHFTESVSLAETARGSGGFGSTGR"
     gene            complement(619..1032)
                     /locus_tag="VFU20_09350"
     CDS             complement(619..1032)
                     /locus_tag="VFU20_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286691.1"
                     /translation="MKRLLALALLLAPVPASSATIVEVADGEWSFLPLMKQRGSDHLN
                     NDALARIDEIIHAHQCTFPDQDGPHYEVTVSFAAHFTAGGALDRLVMKRLNCPEVESL
                     IGGALIEMIEGGDYRPGGENDQGWYRGELSFGGKG"
     gene            complement(1093..2288)
                     /gene="coaBC"
                     /locus_tag="VFU20_09355"
                     /pseudo
     CDS             complement(1093..2288)
                     /gene="coaBC"
                     /locus_tag="VFU20_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003048728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
     gene            complement(2296..>2476)
                     /locus_tag="VFU20_09360"
     CDS             complement(2296..>2476)
                     /locus_tag="VFU20_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011543450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiB"
                     /protein_id="HEU5286692.1"
                     /translation="KLIPRLIHRMDEYYPPTGAAPPPPPLPEIALIERRHWWSYAGIA
                     VAGAAAGAIAMLVLG"
BASE COUNT          348 a          888 c          847 g          393 t
ORIGIN      
        1 gatcgcgccg gcgccgacca gcgcgatgct cgtcgccttc agcttgcgtt gcccggttcc
       61 gccgacctcg cgcaggacga tgtgccgcgc gtagcgctcg agctcggcgt ccgagaggct
      121 caccgtcccg tcgacccgaa ccccccgctg ccgcgcgcgg tctcggcgag gctgacgctt
      181 tctgtgaaat gggcgcggag caccggcgcc ggcaccagct gggcgattcg ctcgccgcgg
      241 cggaccgcga acggctcgga gccgaggttg accaggatca ccttcacttc gccgcggtaa
      301 tcgctgtcga tcgtcccggg cgtgttgagg caggtgatcc cgttgcggat cgcgagcccc
      361 gagcgcgggc ggacctggac ctcgtagcca tgcgggatct cgatcgcgaa gccggtcgcg
      421 accgcgtgcc gctcgccggg cccgagcacc agctcctcgg ccgaggcgat gtcgagcccg
      481 gccgcgtcct cgcttgcata tttgggcagc ggcagcccgt cgccgtgcgg caggcgatac
      541 agcttgatct cgatcattcg gactcccgtg cgaagcggcc ggtacgcgca gggcggatgc
      601 ccgaaaccga cggaagcgtc agcccttgcc gccgaagctc agttcgccgc ggtaccagcc
      661 ctggtcgttc tcgccgcccg gccgatagtc gccgccctcg atcatctcga tcagtgcgcc
      721 gccaatcagg ctctcgactt cgggacagtt gagccgcttc atcaccagcc ggtcgagcgc
      781 cccgcccgcg gtgaaatggg ccgcgaacga cacggtgacc tcgtaatggg gcccgtcctg
      841 gtccgggaac gtgcattggt gggcatggat gatctcgtcg atccgggcga gcgcgtcgtt
      901 gttcagatgg tccgagccgc gctgcttcat cagcggcagg aagctccatt cgccgtcggc
      961 gacctcgacg atcgtcgcgg acgacgccgg aaccggagcg agcagcagtg ccagagcgag
     1021 caagcgcttc atcgttcgat ctcccacaag acaggacgac cttattgcaa gcgggcggcc
     1081 tttgtcgatg attcagcgcc tggagcccag cgcggcggcg atccgcccga ccagccgccg
     1141 ggcgacctcg tccttgcccg cttccggcca gccctcgact ccctgtgcgg tcactaggtg
     1201 cacccggttg cggtggccgc ccatcacgct ggtctccctt ggcccggaca cgtcgttggc
     1261 gacgatccag tcggcgccct tggccgctcg cttggcgatc gcgttggcga ccaattggtc
     1321 ggtctcggcg gcaaagccga ccagcagcgg cgggcggtcc gggtgggcgg ccagctcggc
     1381 gaggatgtcg cgggtcggca cgaagcgcag ctgcggcggg ccgccgcttt tcttgagctt
     1441 tccagccgag ggctcgaccc gccagtcggc gaccgcggcg actaggaccg cggcgtcgca
     1501 gggcaaagcg cctgtcaccg cgtcggccat ctgcgcggcc gtctcgacgt cgactcggtc
     1561 gacaccggcc ggagtttcca gagcgaccgg ccccgcgacg agcgtgactc gggcgccggc
     1621 ttcggcggcg gcggcggcaa ttgcaaaacc ctgccgcccc gacgagcggt tggcgatcac
     1681 ccgcaccggg tcgatcggct cgtgggtcgg cccggccgtg acgaggatgt gcttgccctg
     1741 gagggtcact tgccggtcag gaactcggcg ccgagccact ccatgatcgc cgccggctcg
     1801 ggcagccggc cgggtccgtg ctcgccgcac gccatctcgc cttcgtcggg ctcgatcacc
     1861 tggactccgt ccgccttcaa ttgcgcgacg ttgcgctggg tcgccgggtg cagccacatg
     1921 cgcacgttca tcgccggagc gaccaccacc ggcttgtcgg tcgccagcag cagcgtcgtc
     1981 gcgaggtcgt cggcgagccc gcccgccatc ttcgccagca ggtccgcggt cgccgggcag
     2041 acgagcagga aatccgccga ccggctgagc tcgatatgcc ccatctcgac ttcgtccttg
     2101 aggtcccaga ggctggtgta gaccgggctc ctggccagcg cgcccaaggt catcggagcg
     2161 acgaaatgct cgccgccctt ggtcagcacc ggggtcacct ggtggccggc cttggccagc
     2221 aggcgcacca gctcgcagct cttgtaggcg gcgatcccac ccccgacgat cagcaacacg
     2281 cgcttcatgg ccggctcagc ctagcacaag cattgcgatt gcgcccgccg cggcgccggc
     2341 gaccgcgatt ccggcatagc tccaccagtg gcggcgctcg atcagcgcga tctcgggcag
     2401 cggcggcggc ggaggggcgg caccggtcgg cgggtaatat tcgtccatcc ggtggatcag
     2461 cctggggatc agcttc
//
LOCUS       DASTZO010000332         4025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_335933,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000332 DASTZO010000000
VERSION     DASTZO010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_335933"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..158
                     /locus_tag="VFU20_09365"
     CDS             <1..158
                     /locus_tag="VFU20_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010164046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nicotinate-nucleotide diphosphorylase
                     (carboxylating)"
                     /protein_id="HEU5286693.1"
                     /translation="RVPLEASGGVTLDTIRAIAGTGVDYISVGRITLSAPAVDIGLDY
                     GPARPAG"
     gene            239..1645
                     /locus_tag="VFU20_09370"
     CDS             239..1645
                     /locus_tag="VFU20_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007405037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU5286694.1"
                     /translation="MFKGIRPIVYGGREVWPLIEGGKGVSATNHASSGAWAAAGGVGT
                     VSAVNADSYDPEGRIIPQIYEGMTRRDRHEELIKFAVDGAVAQVTRAYEIANGRGAIN
                     INVLWEMGGAQRVLHGVLERTRGMVTGVTCGAGMPYKLSEIAASYGVNYLPIISSARA
                     FRALWKRAYSKAAEWLAAVVYEDPWLAGGHNGLSNAEDPRQPQDPYPRVKELRETMRE
                     GGIPDSLPIVMAGGVWRLDEWDHWIDNPELGQIAFQFGTRPLLTQESPIPPEWKARLM
                     TLEEGDILLHRFSPTGFYSSAVRNAFLRNLEERSHRQIAFTKEPLGDHAFELDVGIGT
                     KKGYWVTKGDLQHAREWFAAGFTEALKTPDETLVFVTPEEKQHIRRDQAECMGCLSQC
                     GFSSWADNEKNTTGRLADPRSFCIQKTLQDIAHGGPTEHNLMFAGHSAFRFQSDPFYS
                     NGFVPTVKQLVDRILTGA"
     gene            1645..2133
                     /locus_tag="VFU20_09375"
     CDS             1645..2133
                     /locus_tag="VFU20_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU5286695.1"
                     /translation="MARRRVRRGLIAAVLVALAAPAAAQDKTPPYWASIASGEAMMRT
                     GPGRNYPGIWLYKRRDLPVRVVKTYPNWRMIEDPDGARGWMLVSLLSDQRTAIVRPGE
                     PRAIHVEPDPASRVRYRAEHGVVGRIDRCGGVWCRIAVGKRQGYIRMSDIWGVGENEK
                     LD"
     gene            complement(2177..2617)
                     /locus_tag="VFU20_09380"
     CDS             complement(2177..2617)
                     /locus_tag="VFU20_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286696.1"
                     /translation="MHKIILVAAVAALAGCSQETAAPPAEDANVATEANAADANAAEP
                     AAAGTPLASLNETTWEFIDEGKPIRESIDAAGNYIAVSGTEHIDHGTAVMKDGKACFT
                     SAMDKEGEVCWTDPMVAIGESGETTSDKGEKLTVKRVAYVPLTM"
     gene            2819..3178
                     /locus_tag="VFU20_09385"
     CDS             2819..3178
                     /locus_tag="VFU20_09385"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5286697.1"
                     /translation="MLRGRPLPFSDAVRVGDLLFLSGQVGLRGDGTLPDGVVAQARQT
                     MDNIGGVLKAQGLGWSDVVKCTVMLDNIADWPAFNAVYVTYFPDGRFPARSAFGADGL
                     ALGALIELECIAYAGKR"
     gene            complement(3180..>4025)
                     /locus_tag="VFU20_09390"
     CDS             complement(3180..>4025)
                     /locus_tag="VFU20_09390"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5286698.1"
                     /translation="AGGMESMTNAPYLLKKHRSGARIGHDTAYDHMFLDGLEDAYEPG
                     RAMGTFAQETANQYQLTREEMDNYSIESLSRANSAISAGSFKDEIVPVVIQGRGGETV
                     VDTDEAPGRGRPDKIPQLKPAFAKEGSITAATSSSISDGAAAVVLARESTARDKGLEP
                     VARIVATAAHAQEPAQFTVAPIGAIEKVLAKAGWKTADVDLWEVNEAFACVAMFAMKD
                     LGIPHEKINIHGGATALGHPIGASGTRILVTLVNALKQKGLKRGVASLCIGGGEATAV
                     AVELV"
BASE COUNT          628 a         1342 c         1398 g          657 t
ORIGIN      
        1 ggcgagtgcc gctcgaggcg tcgggcggag tcacgctcga caccatccgc gccatcgccg
       61 ggaccggggt cgactatatc tcggtcggcc gaatcacctt gtcggcgccg gcggtcgaca
      121 tcggcctcga ctacggtccc gcgaggcctg ccggctagca actcccgcgc aacatggatt
      181 tatttcgcat ctccggcgtt gggcgattat atggcgccga agctttagga gaataggttt
      241 gttcaagggc atcaggccca tcgtgtacgg cggccgcgag gtgtggccgc tgatcgaagg
      301 cggcaagggc gtcagcgcca ccaatcacgc cagctcgggc gcctgggcgg cggccggcgg
      361 agtcggcacg gtctcggccg tcaatgccga cagctacgac cccgaaggcc gcatcatccc
      421 gcagatctac gaggggatga cgcggcgcga ccggcacgag gaattgatca aatttgcggt
      481 cgacggcgcc gtcgcgcagg tcacccgagc ctatgagatc gccaacgggc gcggagcgat
      541 caacatcaac gtgctgtggg agatgggcgg cgcgcagcgg gtgctgcacg gggtcctcga
      601 gcgcacaagg ggcatggtca ccggcgtcac ctgcggcgcc gggatgccgt acaaattgag
      661 cgagatcgcc gcgtcctacg gggtcaatta cctgccgatc atcagctcgg cgcgcgcctt
      721 ccgggcgctg tggaagcggg cctattccaa ggcggccgag tggctcgccg cggtagttta
      781 cgaggacccg tggctggccg gcgggcacaa cggcctgtcg aacgccgagg acccgaggca
      841 gccgcaggac ccctatccgc gggtcaagga attgcgcgag acgatgcgcg agggcgggat
      901 tcccgacagc ctgccgatcg tcatggccgg cggcgtctgg cggctcgacg aatgggacca
      961 ttggatcgac aatcccgagc tcggccagat cgccttccag ttcgggaccc ggccgctgct
     1021 gacccaggaa agcccgatcc cgcccgaatg gaaggcgcgg ctgatgacgc tcgaggaagg
     1081 cgacatcttg ctgcaccgat tctcgccgac cggcttttat tcgtcggcgg tgcgcaacgc
     1141 cttcctgcgc aacctcgagg aacgctcgca ccgccagatc gctttcacca aggagccgct
     1201 gggtgatcac gcgttcgagc tcgacgtcgg gatcggcacc aagaagggct attgggtgac
     1261 caagggcgac ctgcagcacg cgcgcgaatg gtttgcggcc ggcttcaccg aggccttgaa
     1321 gacgcccgac gaaaccctgg tgttcgtcac tcccgaggag aagcagcaca tccgccgcga
     1381 ccaggccgag tgcatgggct gcctgtcgca atgcggcttc tcgtcgtggg ccgacaatga
     1441 gaagaacacc accggccggc tcgccgaccc gcgcagcttc tgcatccaga agaccctgca
     1501 ggacatcgcc cacggcggcc cgaccgagca caatctgatg ttcgccggcc actcggcgtt
     1561 ccgcttccag agcgacccct tttactcgaa cggcttcgtg ccgaccgtga agcagctcgt
     1621 cgaccggatc ctgaccgggg cgtgatggca cgacggaggg taaggcgagg gctgatcgcg
     1681 gcggtgctgg tcgcgctggc cgcccccgcc gccgcgcagg acaagacccc gccctattgg
     1741 gcgtcgatcg ccagcggcga ggcgatgatg cgcaccggcc cgggccgcaa ttatcccggc
     1801 atctggctgt acaagcgccg cgacctgccg gtgcgggtgg tcaagaccta ccccaactgg
     1861 cggatgatcg aggacccgga cggcgcccgc ggctggatgc tggtgtcgct gttgagcgac
     1921 cagcgcaccg cgatcgtccg gccgggcgag cccagggcca tccacgtcga gcccgacccc
     1981 gccagccggg tgcgctaccg cgccgagcat ggcgtcgtcg ggcggatcga tcggtgcggg
     2041 ggcgtgtggt gccggatcgc ggtcggcaag cgccagggtt atatccggat gagcgacatc
     2101 tggggcgtcg gcgagaacga aaagctcgac tgaccggcgg cgcgcgcttc gcgccaccgg
     2161 accagccgcg gaaaaactac atcgtcagcg gaacgtaggc gacgcgcttg acggtcagct
     2221 tttcgccctt gtcgctggtg gtctcgcccg attcgccgat cgccaccatc gggtcggtcc
     2281 agcagacctc gccttccttg tccatggcgc tggtgaagca ggccttgccg tccttcatca
     2341 ccgcggtgcc gtgatcgatg tgctcggtgc cggaaaccgc aatatagttg ccggccgcgt
     2401 cgatcgattc gcggatcggc ttgccctcat cgatgaattc ccaggtggtt tcgttgagcg
     2461 aggcgagcgg cgtccccgcg gcagcgggct cggccgcgtt ggcgtccgcg gcgttggcct
     2521 ccgtcgcgac attggcgtcc tcggcgggcg gcgccgcggt ttcctgcgaa cagccggcga
     2581 gcgcggcgac ggccgcgacc agaatgatct tgtgcatgtg gcttctcccc tgttgaggac
     2641 ccgcgaacta acagggttcc ggaacgctgg cgaatccgcc accatgacgg tcgcaaagag
     2701 ggaggatgcg atggacctgt tcgttgcggc cgctgcggcc gccacgctgg ccgcgaacgg
     2761 cgccccggcc gaggcgccgc cgaccgagcg cccggccgtc gagcgtatcg gcaagccgat
     2821 gctgcgcggc cggccgctgc cgttttccga cgcggttcgg gtcggcgacc tgctcttcct
     2881 gtcgggccag gtcggccttc gcggagacgg cacgctgccc gacggcgtgg tggcccaggc
     2941 gcgccagacg atggacaata tcggcggcgt cctgaaggcg cagggcctcg gctggagcga
     3001 cgtcgtcaaa tgcacggtga tgctcgacaa catcgccgac tggccggcgt tcaacgcggt
     3061 ctacgtgacc tatttcccgg acggccgctt cccggcgcgc agcgcgttcg gggccgacgg
     3121 cctcgcgctc ggagcgctga tcgagctcga gtgcatcgcc tacgccggga agcgctagcc
     3181 tagaccaatt ccaccgcgac cgcggtcgct tcgccgccgc cgatgcacag cgaggcgacc
     3241 ccgcgcttta ggcccttctg cttcagggcg ttgaccaggg tgacgaggat gcgcgtgccg
     3301 ctggccccga tcgggtggcc caatgcggtc gcgccgccgt ggatgttgat cttctcgtgc
     3361 gggatcccga ggtccttcat cgcgaacatg gcgacgcagg cgaacgcctc attgacttcc
     3421 cacaggtcga cgtcggcggt cttccagccg gccttggcga gcaccttctc gatcgctccg
     3481 atcggggcga cggtgaattg cgcgggctcc tgcgcatggg cggcggtggc gacgatccgg
     3541 gcgaccggct cgaggccctt gtcgcgggcg gtcgactcgc gcgccagaac gaccgcggcg
     3601 gcgccgtccg agatcgacga ggaggtcgcg gcggtgatcg acccttcctt ggcgaaggcc
     3661 ggcttcagct gcgggatctt gtcggggcgg ccgcggcccg gcgcctcgtc ggtgtcgacc
     3721 acggtttcgc cgccgcggcc ctggatcacg accggaacga tctcgtcctt gaacgagccc
     3781 gcgctgattg cggaattggc gcgcgacagg ctctcgatgg aataattgtc catctcttcg
     3841 cgggtcagct gatactggtt tgctgtttcc tgggcgaagg tgcccatcgc ccggcccggc
     3901 tcataggcat cctcgagccc gtcgaggaac atatggtcgt aggccgtgtc gtggccgatg
     3961 cgggcgcccg agcggtgctt cttgagcaaa tagggcgcgt tggtcatcga ctccatgccg
     4021 cccgc
//
LOCUS       DASTZO010000333         3078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_989484,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000333 DASTZO010000000
VERSION     DASTZO010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3078
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989484"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="VFU20_09395"
     CDS             complement(<1..332)
                     /locus_tag="VFU20_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012049305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEU5286699.1"
                     /translation="MVITRFAPSPSGRLHLGHAYSAAIGHSRARQSGGEFRLRIEDLD
                     PARSRPEFVSGIEEDLRWLGLTWGGEPLVQSQRTHEYAAALERLKDRGLVYPCFCTRA
                     DIAASLTAP"
     gene            582..1148
                     /locus_tag="VFU20_09400"
     CDS             582..1148
                     /locus_tag="VFU20_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010162002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU5286700.1"
                     /translation="MYHPELLRHPDSCPALVLNADYTPLSYYPLSLWPWQTAVKSMFL
                     ERVDVVAHYEREVHSPSRALKLPSVIALRQFVRPNQYPAFTRFNLFLRDRFRCVYCGS
                     NKELTFDHVVPRAQGGRTTWENVATACAPCNLKKGGRTPRQAKMHFEREPIRPTSWQL
                     QDHGKSFPPNYLHQSWRDYLYWDVELEP"
     gene            complement(1155..2015)
                     /locus_tag="VFU20_09405"
     CDS             complement(1155..2015)
                     /locus_tag="VFU20_09405"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HEU5286701.1"
                     /translation="MSNQVRKAIFPIAGLGTRLLPATKTVAKEMLTIVDRPLIQYAVD
                     EAREAGIEQMIFVTGRGKSSLVDYFDMAFELETTMRSAGKSLDALEPSRTRFGEVVAV
                     RQQQPLGLGHAVWCARDIVGDEPFAVLLPDDLMAGRPGALKQMADAWASVGGNMLCAE
                     EVPAEKTRSYGVIAPGKVDGGLTEVLGLVEKPDPADAPSRLAVIGRYILQPEVMGVLD
                     DAERGAGGEIQLTDAMARLIGSQPFHALKVDAVRHDCGDKAGFVIANLALALQRDDIA
                     PRVREYLATL"
     gene            complement(2012..2335)
                     /locus_tag="VFU20_09410"
     CDS             complement(2012..2335)
                     /locus_tag="VFU20_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286702.1"
                     /translation="MKFAPVLMLTAGGLLASACGQMAELQPARGEPLPVKPLLARTTP
                     TAEQLLTPPTNADPERIDELVKRSEPRKPDRFDLPPPDGGEAPVLPETQEPSSSEQVG
                     PATPE"
     gene            complement(2332..>3078)
                     /gene="argH"
                     /locus_tag="VFU20_09415"
     CDS             complement(2332..>3078)
                     /gene="argH"
                     /locus_tag="VFU20_09415"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010405411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEU5286703.1"
                     /translation="AALGFDRPTANSVDSVSDRDFVVDYLHAAALCSVHLSRLAEEIV
                     VWASQPFGFVVLPDAWSTGSSIMPNKRNPDAAELVRGHCARIVGDLVALLVLLKGLPL
                     AYAKDLQDDKAPLFDAHDLLMVSLAAMAGMVADIRFDAARMRATAEAGHSTATDLADW
                     LAAEAGVPFREAHHIAGRAVAAAEARGVGLSELTLEELQAIDPRIGAEALGRLSVEAS
                     VAARTSAGGTAPERVIEAIAAARAARRSEG"
BASE COUNT          455 a         1130 c         1040 g          453 t
ORIGIN      
        1 ggagcggtca gcgacgcggc gatgtcggcg cgcgtgcaga agcacggata gaccaggccg
       61 cggtctttca gccgctccag cgcggcggca tattcgtgcg tccgctgtga ctggaccagc
      121 ggctcgccgc cccacgtcag ccccagccag cgcagatcct cctcgatccc ggacacgaac
      181 tccggccggc tgcgcgccgg gtcgaggtcc tcgatccgaa gccggaactc gccgccgctc
      241 tgccgcgccc gcgaatggcc gatcgccgcg ctgtaggcgt ggcccagatg cagccgcccg
      301 ctcggcgacg gagcgaaacg ggtgatcacc atcggtgcgg atgtcccccg gcgaaggccc
      361 ggccccaatg ctctcgtcgg actgggggcc ggctttggcc ggagaagtgt ggcccaagag
      421 actcttgacc cgggggcggc gaaaatgcag tcatcaccat gtcatgccgg gatggcatcc
      481 ggctcgacac aagggaagac gggccgggcc ctctcctcct tgcgtgcgac ggggccgggg
      541 agctgcagtt cggaccaccg aacgaaaggc tcacgcgccc aatgtatcat cccgagctcc
      601 tccgccatcc cgacagctgc ccggcgctgg tgctcaacgc cgactacacg cctttgtcct
      661 actacccgct tagcctttgg ccatggcaga cggcggtcaa atcgatgttc ctcgagcggg
      721 tcgacgtcgt cgcccattac gagcgcgagg tgcacagccc gagccgggcg ctgaagctgc
      781 cgtcggtgat cgctctgcgc cagttcgtga ggcccaacca gtatccggcg ttcacccggt
      841 tcaacctgtt cctgcgcgac cgcttccgct gcgtctattg cggctccaac aaggagctga
      901 ccttcgacca tgtcgtgccg cgcgcccagg gcggccgcac gacctgggag aatgtcgcca
      961 ctgcctgcgc cccgtgcaac ctcaagaagg gcggccggac cccgcgccag gcgaagatgc
     1021 atttcgagcg cgagccgatc cgcccgacca gctggcagtt gcaggaccac ggcaagagct
     1081 tcccgcccaa ctacctccac cagagctggc gcgattatct ctactgggac gtcgagctgg
     1141 agccgtaacc ggcctcacag cgtcgcgaga tattcccgca cccggggggc gatgtcgtcg
     1201 cgctggagcg cgagcgcgag gttggcgatc acgaacccgg ccttgtcgcc gcaatcgtgg
     1261 cgcaccgcgt cgaccttcaa cgcgtggaac ggctggctcc cgatcagcct ggccatcgcg
     1321 tcggtcagct ggatctcgcc gccggcgccg cgctcggcgt cgtcgagcac ccccatcacc
     1381 tcgggctgga ggatgtagcg gccgatcacc gccagccgcg acggcgcatc cgccgggtcc
     1441 ggcttctcga ccaggccgag cacctcggtc aggccgccgt cgaccttgcc cggagcaatc
     1501 actccgtagc tccgggtctt ctcggccggc acttcctcgg cgcacagcat gttgccgccg
     1561 acgctcgccc aagcgtcggc catctgcttc agcgccccgg gccggccggc catcagatcg
     1621 tcgggcagga gcaccgcgaa cggctcgtcg ccgacgatgt cgcgcgcgca ccacaccgcg
     1681 tggccgagcc cgagcggctg ctgctggcgg accgcgacca cctcgccgaa gcgggtccgc
     1741 gacggctcga gcgcatcgag gctcttgccg gcgctcctca tcgtcgtttc cagttcgaac
     1801 gccatgtcga aatagtcgac caggctcgac ttgccgcggc cggtgacgaa gatcatctgc
     1861 tcgatccccg cctcgcgcgc ctcgtcgacc gcgtactgga tcagcggccg gtcgacgatc
     1921 gtcagcatct ccttggccac cgtcttggtc gccggaagca ggcgcgtccc gagcccggcg
     1981 atcgggaaga tggccttgcg gacctgattg ctcattcggg ggtcgccggg cccacttgct
     2041 cggacgagct cggctcctgg gtctccggga gcaccggcgc ttcaccgccg tcgggcggcg
     2101 gcaggtcgaa gcggtcgggc ttgcgcggct cggagcgctt gaccagctcg tcgatccgct
     2161 ccgggtcggc gttggtcggc ggggtcagca attgctcggc ggtcggggtc gtccgggcga
     2221 gcagcggctt gaccggcagc ggctcgccgc gcgccggctg cagctcggcc atctggccgc
     2281 aggcgctcgc cagcaggccg ccggcggtca gcatcagcac gggcgcgaac ttcatccttc
     2341 actccggcgg gcggcgcggg ccgcggcgat cgcctcgatt acccgctcgg gcgcggttcc
     2401 gccagccgag gtgcgcgccg cgacgctggc ctcgaccgac agccgcccga gtgcctcggc
     2461 gccgatgcgc gggtcgatcg cctgcagctc ctcaagcgtc agctcgctca gtccgacgcc
     2521 cctggcctcg gccgccgcga ccgcccgccc ggcgatatgg tgcgcctcgc ggaacgggac
     2581 cccggcctcg gccgccagcc agtcggcaag gtcggtcgcg gtcgaatggc cggcctcggc
     2641 ggtggcgcgc atccgggccg cgtcgaagcg gatgtccgcc accatcccgg ccatcgccgc
     2701 cagcgacacc atcagcagat cgtgcgcgtc gaacaacggc gccttgtcgt cctgcaagtc
     2761 cttggcatag gcgagcggca ggcccttgag cagcaccagc agcgcgacca ggtcgccgac
     2821 gatccgcgcg caatggccgc ggaccagctc ggccgcgtcg gggttgcgct tgttgggcat
     2881 gatcgacgag cccgtcgacc aggcgtcggg caggacgacg aacccgaacg gctgcgacgc
     2941 ccagacgacg atctcctcgg ccaggcgcga cagatgcacc gagcacaggg ccgcggcgtg
     3001 caggtaatcg acgacgaagt cgcggtcgga gaccgaatcg acgctgttgg cggtcggccg
     3061 gtcgaagccg agcgccgc
//
LOCUS       DASTZO010000334         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_76207,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000334 DASTZO010000000
VERSION     DASTZO010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76207"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VFU20_09420"
     CDS             <1..499
                     /locus_tag="VFU20_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucose/galactose MFS transporter"
                     /protein_id="HEU5286704.1"
                     /translation="SILVNYLIQADTLATTAERAGQMVAFYWGGAMVGRFIGSGVLRL
                     VSPGLVLLGVAVGAGLLTSTSALTLGTISGIALIAVGFMNSIMFPTIFSLASEDLGRR
                     AADGSGIICVAIVGGAIVPAITGFVADLTSLRAALFVPVACYVVIAAFGVWCFNARRG
                     ERRTA"
     gene            complement(496..1524)
                     /locus_tag="VFU20_09425"
     CDS             complement(496..1524)
                     /locus_tag="VFU20_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010186234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5286705.1"
                     /translation="MTQRRPTSFDIAALAGVSQPTVSRALSGNPSVSEPTRARVLAAA
                     EQLNYKVDKNASGLRRQQSRTLALLFFEERSADGALINPFYLSMLGPMVRKCATLGYD
                     LLISFQQLSSDWHVDYEDSRKADGIILLGYGDYLEYQPLLQRLVERGTHFVRWGPAGG
                     VQFGATVSSDNEQGGFDATQHLLQVGRRRIAFIGTAEPAYPEFLDRWGGHCRALRASG
                     MEPDERLRADAEPSEESGRAAVADLLGRGVEFDAIFAASDVTAIGAMHALQKLGRAIP
                     QEVAIVGFDDIPAASLSSPPLSTVTQDARGAGEALVEALIEAIELGAARSRVLPVHLT
                     VRESSRPA"
     gene            1685..>3241
                     /locus_tag="VFU20_09430"
     CDS             1685..>3241
                     /locus_tag="VFU20_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011413600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEU5286706.1"
                     /translation="MKPISTRSQFAATVSPLAIGLAMLLSAGAAQAQDTGTPPPAEPP
                     QQTEEEKTEVTPTGEVQEDISAGGTTSPSGDSQDIVVTGFRASLMNAINKKKTSEQVV
                     ESVSAEDIGKLPDASIGESIARLPGLTSQRLNGRAAFISIRGFGPDFSTTLLNGREQT
                     STGDTRAVEFDQYPSEVVNRVDVYKTMTPSLIGQGLVGTVDIRTARPLETSSRVIAVG
                     LKASNAEIGKLNAGSKDFGWRANATFIDKFADDTMGIALSASYVDEPYQIEEYNAWGW
                     QDFGGRIISGNKSWVTSTQLKRYGASATFQADVSDAFRVTLDGFYSKFDDDIIRRGIE
                     LPLAGAFGWTSAGLSNAQVEDGNVVSGTFTDIEAVVNNHHLTREADLVSGGANLAWRP
                     GNGWKGELDISFSRTDREELIFESNAGTGRGQGVGARDSLDFDCDESGCTFDPTLNYS
                     DPNVIQLTSPMGWGAGPDTPGGQDGYYNNRIVDDELKQYRADVERELGGFLSSVRAGL
                     SYTDRDKSLTP"
BASE COUNT          540 a         1107 c         1096 g          498 t
ORIGIN      
        1 cagcatcctc gtcaattatc tgatccaggc ggacacgctc gccaccaccg ccgagcgcgc
       61 cgggcagatg gtcgccttct actggggcgg ggcgatggtc ggccggttca tcggctcggg
      121 cgtgctgagg ctggtgtcgc ccgggctggt gctgctcggg gtcgcggtcg gagcggggct
      181 cctgacctcg acctcggcgc tgaccctggg cacgatctcc ggaatcgcgc tgatcgcggt
      241 cgggttcatg aattcgatca tgttcccgac catcttcagc ctcgccagcg aggacctcgg
      301 gcgccgcgcc gcggacggca gcgggatcat ctgcgtcgcc atcgtcggcg gcgccatcgt
      361 tccggcgatc accggcttcg tcgccgacct caccagcctg cgcgcggcgc tgttcgtgcc
      421 ggtcgcctgc tatgtggtca ttgccgcgtt cggggtgtgg tgcttcaatg cgcggcgcgg
      481 cgagcgccga acggcctagg ccggccggga cgactcccgc accgtcaggt ggaccggcag
      541 cacccggctc cgcgccgcgc cgagctcgat cgcctcgatc agcgcctcga ccagcgcctc
      601 gccggcgccc ctcgcgtcct gggtgacggt gctcagcggc gggctcgaca ggctcgccgc
      661 cgggatatcg tcgaagccga cgatcgccac ttcctgcggg atcgcccggc cgagcttctg
      721 gagcgcgtgc atcgccccga tcgcagtgac gtcgctggcg gcgaaaatcg cgtcgaactc
      781 gactccgcgc ccaagcaggt cggcgaccgc ggcgcggccc gattcctcgc tcggctcggc
      841 atccgcgcga agccgctcgt cgggctccat cccggacgcg cgcagcgccc ggcaatgccc
      901 gccccagcgg tcgaggaatt ccgggtaggc gggctcggcg gtgccgatga acgcgatcct
      961 gcggcggccg acctggagca gatgctgggt cgcgtcgaac ccgccttgct cgttgtcgga
     1021 gctgacggtg gctccgaatt gcactccgcc ggccggcccc cagcgcacga aatgcgtgcc
     1081 gcgctcgacg agccgctgga gcagcggctg atattcgaga tagtcgccgt aaccgagcag
     1141 gatgatcccg tcggccttgc ggctgtcctc ataatcgacg tgccagtccg acgagagctg
     1201 ctggaacgag atcagcaggt cgtagccgag cgtcgcgcat ttgcgcacca tcgggccgag
     1261 catcgacagg tagaaagggt tgatcagcgc gccgtcggcc gagcgctcct cgaagaacag
     1321 cagcgccaag gtccgcgact gctggcggcg cagtccggac gcgttcttgt cgaccttgta
     1381 attgagctgc tcggcggcgg cgaggacgcg cgcccgggtc ggttcgctga ccgacggatt
     1441 gccgctcaag gcgcgcgaaa ccgtcggctg cgacacgccg gccagcgccg cgatgtcgaa
     1501 ggacgttggg cgtctttgag tcatcaaacg cagagcctaa gcgcggtgaa tacgtatgta
     1561 tagccgcgca aggcttccgg cgcggcaaac tactgatatt ccttggcggg tggagagcga
     1621 ggccgggggc cgcgcattgg gggctcgtgc cgcgacgcgg gacgagtttc aggggagcga
     1681 caaaatgaag ccaatctcga cgcgttcgca gttcgcagcg accgtaagcc cgctggcgat
     1741 cggcctggcg atgctgctga gcgccggcgc cgcccaggcg caggacacgg gtactccgcc
     1801 gccagccgag ccgccgcagc agaccgagga agaaaagacc gaagtgactc cgaccggcga
     1861 ggtccaggag gacatttccg cgggcggcac gacctcgcct tcgggagatt cccaggacat
     1921 cgtcgtcacc ggcttccggg ccagcctgat gaacgcgatc aacaagaaga agaccagcga
     1981 gcaggtcgtc gaatcggttt cggctgagga tatcggcaag ctgcccgacg cgtcgatcgg
     2041 cgagtcgatc gcgaggcttc ccggcctgac gtcgcagcgg ctcaacggcc gcgccgcctt
     2101 catctccatc cgcggtttcg gccccgactt ctcgaccacc ttgctcaatg gccgcgagca
     2161 gacctcgacc ggcgacaccc gcgcggtcga gttcgaccaa tatccgtccg aggtcgtcaa
     2221 ccgggtcgac gtctacaaga cgatgacccc gagcctcatc ggccagggcc tggtcggaac
     2281 ggtcgacatc cgcaccgccc ggccgctgga gacgagcagc cgcgtgatcg cggtcggcct
     2341 caaggccagc aacgccgaga tcggcaagct caacgccggc tccaaggatt tcggctggcg
     2401 cgccaacgcc accttcatcg acaagttcgc cgacgacacg atgggcatcg ccctttcggc
     2461 gagctacgtc gacgagccct accagatcga agagtataac gcgtggggct ggcaggactt
     2521 cggcggccga atcatcagcg gcaacaaatc gtgggtcacc tcgacccagc tgaagcgcta
     2581 cggcgccagc gcgactttcc aggccgacgt gtcggacgcg ttccgggtca cgctcgacgg
     2641 cttctattcg aagttcgacg acgacatcat ccggcgcggc atcgagctgc cgcttgcggg
     2701 cgcattcggc tggacctcgg cggggcttag caacgcgcag gtcgaggacg gcaacgtcgt
     2761 cagcggaacc ttcaccgaca tcgaggcggt ggtgaacaac caccatctga cccgcgaggc
     2821 ggacctggtt tccggcggcg cgaacctggc ctggcggccg ggcaacggct ggaagggtga
     2881 gctggacatc agtttctccc gcaccgaccg cgaggagctg atcttcgaat cgaacgccgg
     2941 caccggccgc gggcagggag tcggcgcgcg cgattcgctc gacttcgact gcgacgagag
     3001 cggctgcacg ttcgacccga cgctcaacta cagcgatccc aacgtcatcc agctcacgtc
     3061 gccgatgggc tggggggcgg ggccggacac cccgggcggc caggacggct attacaacaa
     3121 ccgaatcgtc gacgacgagc tcaagcagta tcgcgccgat gtcgagcgcg agctcggcgg
     3181 cttcctttcg agcgttcggg cgggcctgtc ctacaccgac cgcgacaagt ccctgacccc
     3241 g
//
LOCUS       DASTZO010000335         4507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_834155,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000335 DASTZO010000000
VERSION     DASTZO010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834155"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1285
                     /gene="glmS"
                     /locus_tag="VFU20_09435"
     CDS             <1..1285
                     /gene="glmS"
                     /locus_tag="VFU20_09435"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008071502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU5286707.1"
                     /translation="GYGEGENYLGSDAIAVAPWTQRIAYLEEGDWAVVTRDEIEIFDR
                     DNERVEREIVESGASSAPVEKGNYRHYMQKEIFEQPIVVAQTLHSYVRPFEGEVALPD
                     FDFDLAAVERLTIVACGTSYYAGLVAKYWIEQFARVPVDIDVASEFRYRDPVLEPGGL
                     ALFVSQSGETADTLAALRHARAQGQRIAVVVNVPTSSMAREADLLLPTHAGPEIGVAS
                     TKAFTCQLAVLAALAANLARAKGRLSRDEERAIVGHLQEAPAALNAALNHDEEIAGMA
                     HLIAPARDVLYLGRGPDYAMALEGALKLKEISYIHAEGYAAGEMKHGPIALIDEDVPV
                     IVLAPSGPLFEKTVSNMQEVRARGGKIVLISDAKGIAAAGEGTMATIEMPEVHPLIAP
                     LVYAVPVQLLAYHVAVLKGTDVDQPRNLAKSVTVE"
     gene            1374..2243
                     /locus_tag="VFU20_09440"
     CDS             1374..2243
                     /locus_tag="VFU20_09440"
                     /inference="COORDINATES: protein motif:HMM:NF017532.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HEU5286708.1"
                     /translation="MGGSQPLRIADEVGENPFLEHYWREGYAIVRGLFSRSEIDEIAA
                     VADAIHAEGVAHGRCFRHGNLFYNVATRAGGEPLVRMVQWPSYHQAALNRVRLDQRLA
                     RLLEPLIGRNLKQVINQVHWKAPGALGDFAWHQDSRSRRPGWAYRNLADSYVQTGLAI
                     DPHTEESGCMRFLPRSHRRGDLGMDCSKKALGTAMTDDALEAVGLSAADAIELLLEPG
                     DLAVWSPYLVHGSGTNRSQHRRRFYINGYVRAADCDRGEWAFRNGSPVPFGPSPALVH
                     YEELHERPGPHYP"
     gene            complement(2258..3205)
                     /locus_tag="VFU20_09445"
     CDS             complement(2258..3205)
                     /locus_tag="VFU20_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5286709.1"
                     /translation="MLRYHSAKMQNDPMLDWNDLRYFLAVARGGSTLAASRALRVSQT
                     TVARRIAALEEAVKLTLFEKRQAGYALTPAGVELLAHAEAVEAAALRFDSAASASTRD
                     LGGTVRITTEEIFAVTLLAPMLRELHELHPEILIELDTAQAIRDLGAGEADIAMRSTV
                     QADGNGLVGRRLCRDDWTLYCSRDYAARNGVPASLEDLKNHAIIGGGGGNLWRHYQAW
                     LRSLGLEDQVAMHHASSTGLLSGVRSGFGIAVLPCVVADAEADLIRCLPPRSDHDRTF
                     WLLTHERVRNAPRVRTVIDFLYERLRRHVRELEGRRAAA"
     gene            3286..3624
                     /locus_tag="VFU20_09450"
     CDS             3286..3624
                     /locus_tag="VFU20_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UrcA family protein"
                     /protein_id="HEU5286710.1"
                     /translation="MTRALYIALASALITTAAIKAAPALAQAPGGETSISLVRTADLD
                     LGSEAGRRQLDQRLAHAAREVCGTASDFDVEGKNAVRKCRGETLAKARARLESVVAER
                     RAVIAVTANR"
     gene            3704..4081
                     /locus_tag="VFU20_09455"
     CDS             3704..4081
                     /locus_tag="VFU20_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020480511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HEU5286711.1"
                     /translation="MIEAKGGCHCKAVRFTAALPDPPVPAIDCNCSICRMTGFIHIIV
                     PHADFTLDKGSDALTSYRFGTGAAEHLFCSKCGVKSFYQPRSHPGSWSVNAHCLDEPV
                     ELVVDWFDGQNWEEAASSLNRSP"
     gene            4167..>4507
                     /locus_tag="VFU20_09460"
     CDS             4167..>4507
                     /locus_tag="VFU20_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010404129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU5286712.1"
                     /translation="MHRPLRLTLDRAALQHNWRWLAETAGTECGAAIKADGYGLGARE
                     AMAALHEAGCRTFYVSNWAEAAELGELPDGADLAVLHGVGPDDVAAALASAARPVLNS
                     AEQAARWKEIAP"
BASE COUNT          744 a         1484 c         1583 g          696 t
ORIGIN      
        1 cggctatggc gagggagaaa attacctggg atcggacgcg atcgcggttg cgccctggac
       61 ccagcggatc gcctacctcg aggaaggcga ctgggcggtc gtgacgcgcg acgaaatcga
      121 gattttcgac cgggacaacg agcgagtcga gcgcgagatc gtcgaatcgg gcgcgtcctc
      181 cgcgccggtc gagaagggca attaccgtca ctacatgcag aaggagattt tcgagcagcc
      241 gatcgtggtt gcccagactc tccacagcta tgtccgcccg ttcgaagggg aagtcgcgct
      301 gcccgatttc gacttcgacc tggcggccgt cgagcggctg acgatcgtcg cctgcgggac
      361 cagctattat gcgggcctcg tcgcgaaata ctggatcgag cagttcgccc gagtgccggt
      421 cgacatcgat gtcgcgtccg agttccgcta ccgcgacccg gtgctagagc cgggcgggct
      481 tgcgctgttc gtcagccagt cgggcgagac cgcggacacg cttgcggctc tccgccacgc
      541 gcgtgcgcag ggccagcgca ttgcggtggt cgtcaacgtc ccgaccagct cgatggcgcg
      601 cgaggccgat ctgctgctgc cgacccacgc cgggcccgag atcggggtcg cctcgaccaa
      661 ggcgttcacc tgccagctgg cggtgctggc ggcgctggcc gcgaacctgg cgcgggccaa
      721 ggggcggctg tcgcgcgacg aggaacgcgc catcgtcggc catttgcagg aagcgccggc
      781 ggcgctgaac gcggcgctca accatgacga ggagatcgcc ggcatggcgc atttgatcgc
      841 gccggcccgc gacgtgctct atctcggccg cggccccgac tatgcgatgg cgctcgaagg
      901 cgcgctgaag ttgaaggaaa tcagctacat ccatgccgag ggctatgccg ccggcgagat
      961 gaagcacggg ccgatcgcgc tcatcgacga ggacgtgccg gtgatcgtgc tggcgccgtc
     1021 cgggccgctg ttcgagaaga ccgtcagcaa catgcaggaa gtgcgcgcgc ggggcggcaa
     1081 gatcgtcctg atcagtgacg caaaaggcat tgccgcggcg ggcgagggga cgatggctac
     1141 catcgagatg cccgaggtcc acccgctgat cgcgcccttg gtctacgcgg tgccggtgca
     1201 gctgctcgcc taccatgtcg cggttctgaa aggcaccgac gtcgaccagc cgcgcaattt
     1261 ggcgaaaagc gtgacggtcg aataagccgc ggccgccgag ggctgcggcg gccagaaatt
     1321 catggaaatg tcaaagagtt gctgtatctt cgttccgggg cgggagtcgg agcatgggtg
     1381 gttcgcagcc actgaggatc gctgacgagg tcggcgaaaa tccatttctc gagcattatt
     1441 ggcgtgaggg ctatgcgatc gtccgcgggc tgttcagccg ctcggagatc gacgaaattg
     1501 ccgcggtcgc ggatgcgatc catgccgagg gcgtcgcgca cggccgctgc ttccggcacg
     1561 gcaatctgtt ctacaatgtg gccaccaggg ccggcggaga gccgctggtg cggatggtcc
     1621 agtggccgtc ctaccaccag gcggcgttga accgggtccg cctcgatcag cggctcgcgc
     1681 gcctgctcga gccgctgatc gggcgcaatc tcaagcaggt gatcaaccag gtccactgga
     1741 aggcgccggg cgcgctcggc gacttcgcct ggcaccagga cagccggtcg cgccggccgg
     1801 gatgggcgta ccgcaacctt gccgattcct atgtgcagac cggcctcgcg atcgacccgc
     1861 acaccgagga atcgggctgc atgcgcttcc ttccacgcag ccacaggcgc ggcgacctcg
     1921 gcatggactg ttcgaagaag gcgcttggga cggcgatgac cgacgacgcg ctcgaagccg
     1981 tcgggctgtc ggcggcggac gcgatcgagc tgttgctcga gcccggcgac ctggcggtct
     2041 ggagccccta tctggtgcac ggctcgggca ccaaccggtc acagcacagg cgccgctttt
     2101 acatcaacgg ctatgtccgg gcggcggatt gcgaccgcgg cgaatgggca ttccggaacg
     2161 gcagccccgt cccgttcggg ccgagcccgg ctctcgtcca ttacgaggag ctgcacgagc
     2221 gtcccgggcc gcattatccg taaagacgcg aggacgttca agccgcggcg cgccggccct
     2281 ccagctcccg cacgtgccgc ctcagccgct cgtagaggaa atcgatcacg gtccggaccc
     2341 gcggcgcgtt gcggacccgc tcgtgggtca gcagccagaa ggtccggtcg tggtcggagc
     2401 gcggcggcag gcagcggatc aaatcggctt ccgcatcggc gacgacgcac ggcaggacgg
     2461 cgatcccgaa gccggagcgg acgcccgaca gaagccccgt cgacgaggcg tggtgcatcg
     2521 ccacctggtc ctcgagcccg aggctgcgca gccaagcctg gtaatggcgc cacagattgc
     2581 cgccgccgcc gccgatgatc gcgtggttct tcaagtcctc gaggctggcg gggactccgt
     2641 tgcgggccgc ataatcgcgg ctgcagtaga gcgtccaatc gtcccggcac agccgccggc
     2701 cgacgagacc gttgccgtcg gcctggacgg tgctgcgcat ggcgatgtcg gcctcgccgg
     2761 cgccgaggtc gcggatcgcc tgggcggtgt cgagctcgat caggatctcg gggtggagct
     2821 cgtggagctc gcggagcatc ggcgcgagca gagtgacggc gaaaatctcc tcggtggtga
     2881 tgcgcacggt gccgccgagg tcgcgggtgc tggccgaggc ggcgctgtcg aagcgcagcg
     2941 cggcggcctc gaccgcctcg gcatgagcga gcagttcgac tccggccggg gtcagcgcgt
     3001 agccggcctg gcgtttctca aagagggtca gcttcaccgc ctcctcgagc gcggcgatcc
     3061 ggcgggcgac ggtggtctgg ctgacccgga gcgctcggct ggcggcgagc gtgcttccgc
     3121 cgcgggcgac ggcgaggaaa tatctgaggt cgttccagtc gagcatcgga tcgttctgca
     3181 tctttgcaga atggtagcgc aacatcgttg ctcacgtaag ttgcaatttg cgactaaata
     3241 gacggtcgcg ccggggacac cggccctcga caggaggaag cgaccatgac acgagctctc
     3301 tacatcgcgc tggcatcggc gctgatcacc accgcggcga tcaaggccgc acccgctctc
     3361 gcgcaggcgc ccggcggcga aaccagcatc agcctggtcc ggaccgccga cctcgacctc
     3421 ggcagcgagg ccggccggcg ccagctcgac cagcggctcg cccacgccgc gcgcgaagtg
     3481 tgcgggaccg cgtccgactt cgacgtcgaa ggcaagaacg cggtccgcaa atgccgcggc
     3541 gagacgctcg ccaaggcgcg tgcgcggctc gaatcggtcg tcgccgaacg ccgcgcagtg
     3601 atcgcggtca ccgccaaccg ctgagtttcc ccacggaacg gcgccgactc cccagggcgc
     3661 cgttccgctt gtccctgccg cgcagccgcc ctagcgtcgc ttgatgatcg aggcgaaagg
     3721 cggctgtcat tgcaaggcgg tgcggttcac cgcggcgctg cccgatccgc cggtgccggc
     3781 aatcgactgc aattgctcga tctgccggat gaccggcttc atccacatca tcgtcccgca
     3841 tgcggatttc acgctggaca agggcagcga cgccctgacc agctaccgct tcggcaccgg
     3901 cgcggcggag catctgttct gctcgaagtg cggggttaag agcttctacc agccgcgctc
     3961 gcacccgggt tcgtggagcg tcaacgcgca ttgcttggac gagcccgtcg agctggtcgt
     4021 cgactggttc gacggtcaga actgggagga agcggcatcc agtttgaacc gttcgccctg
     4081 agcttgtcga agggctgtct ttcgtcaggg ccgccgacca gggcaggccc tcgacaagct
     4141 cgggacgaac ggtgaaaggc ccaaccatgc accgcccgct tcgattgacg ctcgaccgag
     4201 ccgcgctcca gcacaactgg cgctggctcg ccgagaccgc cgggaccgag tgcggtgcgg
     4261 cgatcaaggc cgacggctat ggccttggcg cacgcgaggc gatggcagcg ctgcacgagg
     4321 ccggctgccg caccttctac gtatccaatt gggccgaggc ggcggagctg ggcgaactgc
     4381 cggacggcgc ggacctcgcg gtattgcacg gagtcgggcc ggatgacgtc gcggcggcgc
     4441 tcgcctcggc cgcgcggccg gtgctcaaca gcgccgagca ggccgctcgg tggaaggaaa
     4501 tcgcccc
//
LOCUS       DASTZO010000336         2327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_834301,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000336 DASTZO010000000
VERSION     DASTZO010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 2327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2327
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834301"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(109..339)
                     /locus_tag="VFU20_09465"
     CDS             complement(109..339)
                     /locus_tag="VFU20_09465"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEU5286713.1"
                     /translation="MPSAVIRRFDYSPERRELAVEFVTGRRYLYCDVPEQEARSLRAA
                     FAKGRYFNAHIRDRYRFRELAAGAEEASATRR"
     gene            complement(355..606)
                     /locus_tag="VFU20_09470"
     CDS             complement(355..606)
                     /locus_tag="VFU20_09470"
                     /inference="COORDINATES: protein motif:HMM:NF025005.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HEU5286714.1"
                     /translation="MSEATPIRPASTVIRGAWYLPRRRQLDLLFTSGRRYVYSGVPMA
                     VAVGFAEAESKGRFYNREIRNRFPCREVGRDSGKERMAA"
     gene            complement(684..1112)
                     /locus_tag="VFU20_09475"
     CDS             complement(684..1112)
                     /locus_tag="VFU20_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5286715.1"
                     /translation="MTDLSATIEALSDRWMQAWVRKDRAVLEESLAPDFALVVSANPT
                     RRLERADWLATACTRYTCTEFRYRDVQVRDLGGGIAAMSAIAEFSAEIDGIPRCGPLF
                     LTDVWRRGEDGRWRVCARYSSSPEPPGESGSSVQALNASG"
     gene            complement(1138..>2327)
                     /locus_tag="VFU20_09480"
     CDS             complement(1138..>2327)
                     /locus_tag="VFU20_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007164832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEU5286716.1"
                     /translation="NYSRFLTGRLPGEPPPTLFEYLPDNALLFVDESHQTVPQIGAMA
                     RGDHRRKITLAEYGFRLPSCIDNRPLRFNEWEAMRPQTTFVSATPGPWELNETGGVFS
                     EQVIRPTGLIDPPVEIKPVEDQVDDLVHEAKETARKGYRTLVTTLTKRMAEDLTEYLH
                     EAGLKVRYMHSDVETLERIELIRELRLGVYDVLVGINLLREGLDIPECGLVAILDADK
                     EGFLRSETSLIQTIGRAARNVDGRVILYADTVTGSIERALNETDRRRAKQLAYNAEHG
                     ITPESIKRQIHDVMAHVTGREGLVVDTGDEERPHLVGHNLKAYLADLEQRMRKAAADL
                     EFEEAARLRDEIRRLEEDDLGIPHPQRVAPRPVGKSSEGKPGTRKGRFGKQQRTRFGG
                     RRG"
BASE COUNT          331 a          797 c          799 g          400 t
ORIGIN      
        1 tcgtgcgatc gacggtttca cgatcgcgat cgttgtcgga catgtaactc tccctgctga
       61 gctgttattg tggcgaaacg aacgaaccgc cggcggcgcg gttccgcttt atcgccgcgt
      121 cgcgctcgcc tcctcggctc cggcggccag ctcgcggaag cggtagcggt cgcggatatg
      181 ggcgttgaaa tagcgcccct tggcgaaggc ggcgcgaagc gagcgcgctt cctgctcggg
      241 gacgtcgcaa tagaggtagc ggcggccggt gacgaactcg accgccagct cgcggcgctc
      301 aggcgaatag tcgaagcggc ggatgacggc cgacggcatg gagcgacaac gatttcaggc
      361 cgccatgcgc tcctttcccg aatcgcggcc cacttcccgg cacggaaagc ggttgcggat
      421 ttcgcggttg tagaaccgtc ctttcgattc cgcctcggcg aagccgacgg cgaccgccat
      481 cggcacgccc gaatagacgt agcgccggcc gctggtgaac agcaggtcga gctgccgccg
      541 ccgcggcagg taccaggcgc cgcggatgac cgtgctcgcg gggcggatcg gggtcgcttc
      601 cgacatgccg ccgcaacgac gatcgggtcg gaaaggattc gcggcttgcg acgaagcgaa
      661 tcgggagcga gtcgagccga gcgctagccg cttgcgttga gcgcctgcac cgacgagccc
      721 gactctcccg gcggctcggg actcgagctg tagcgcgcgc acacgcgcca gcgcccgtct
      781 tccccgcgcc gccagacgtc ggtcaggaac aaggggccgc agcgcgggat tccgtcgatc
      841 tcggcgctga actcggcgat cgccgacatc gccgcgattc cgccgcccag atcgcgcacc
      901 tgcacgtcgc ggtagcggaa ctcggtgcag gtgtagcgcg tgcaggcggt cgccagccag
      961 tcggcgcgct cgagccgccg ggtcgggttg gccgagacga ccaatgcgaa gtccggcgcg
     1021 agcgattcct cgagtacggc ccggtccttc cgcacccacg cctgcatcca gcggtcggac
     1081 agcgcctcga tcgtcgcgga caggtcggtc atggtccctc cgttggtgtc gggccgccta
     1141 gccccgccgc ccgccgaacc gggtccgctg ctgcttgccg aagcggccct tgcgggtgcc
     1201 gggcttgccc tcgctcgatt tgcccaccgg ccgcggcgcg acccgctgcg ggtgcgggat
     1261 cccgaggtcg tcctcctcga gcctgcggat ctcgtcgcgc agccgcgccg cttcctcgaa
     1321 ctcgaggtcc gcggccgcct tgcgcatgcg ctgctcgagg tcggcgagat aggccttgag
     1381 gttgtgcccg acgaggtgcg gccgctcctc gtcgccggtg tcgaccacca gcccctcgcg
     1441 ccccgtcacg tgcgccatga cgtcgtggat ctggcgcttg atgctctcgg gcgtgatgcc
     1501 gtgctcggcg ttgtaggcga gctgcttggc gcgccgccgg tcggtctcgt tcagcgcccg
     1561 ctcgatcgac ccggtgacgg tgtcggcgta gaggatcacc cgcccgtcga cgttgcgcgc
     1621 cgcccggccg atggtctgga tcagcgacgt ctcggagcgc aggaagcctt ccttgtcggc
     1681 gtcgaggatc gcgaccagcc cgcactcggg gatgtcgagc ccctcgcgca gcaggttgat
     1741 cccgaccagc acgtcgtaga cgccgagccg aagctcgcgg atcagctcga tgcgctccag
     1801 ggtctcgacg tccgagtgca tgtagcggac cttcagcccc gcctcgtgga gatattcggt
     1861 cagatcctcg gccatccgct tggtcagggt cgtgaccaaa gttcggtagc cctttcgggc
     1921 cgtctccttg gcttcgtgga cgaggtcgtc gacctggtcc tcgaccggct tgatctcgac
     1981 cggcgggtcg atcaggccgg tcggccggat cacctgctcg gaaaagactc cgccggtctc
     2041 gttcaattcc cacgggcccg gggtcgccga gacgaaggtg gtttgcgggc gcatcgcctc
     2101 ccattcgttg aacctgagcg gccggttgtc gatgcagctc ggcagccgga atccatattc
     2161 ggcgagcgtg atctttcgcc ggtggtcgcc gcgcgccatc gcgccgatct gcggcaccgt
     2221 ctggtggctc tcatcaacga acaggagcgc attgtccggc agatattcga acaaagtcgg
     2281 cggcggctcg cctggcagcc gaccggtgag gaagcggctg taattct
//
LOCUS       DASTZO010000337         4489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_990976,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000337 DASTZO010000000
VERSION     DASTZO010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 4489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4489
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990976"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..360)
                     /locus_tag="VFU20_09485"
     CDS             complement(<1..360)
                     /locus_tag="VFU20_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015460335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO family protein"
                     /protein_id="HEU5286717.1"
                     /translation="MTSAVPYRPDPKILELGPDFYDVVRPATFPKAIPRFLNRRWAER
                     VGLNLDDRQWEAHFCRFEPLPDNLEQPLALRYHGHQFRAYNPDIGDGRGFLFAQMRDE
                     NDRLLDLGTKGSGTTPYS"
     gene            complement(432..866)
                     /locus_tag="VFU20_09490"
     CDS             complement(432..866)
                     /locus_tag="VFU20_09490"
                     /inference="COORDINATES: protein motif:HMM:NF017079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HEU5286718.1"
                     /translation="MTAFRRWGGRAAAGLLLLAASTAASAECEVSPQEVDFGAYDPLS
                     ATALNGVGNVQVTCDLPTSFVVSLGPGTGTVANRRMTGGAAQLNYNLYKDAARLFVWG
                     EGLAGVSTLGTNVQLTVYGRIPAGQTVPADVYVDSVAVTVDF"
     gene            complement(863..3193)
                     /locus_tag="VFU20_09495"
     CDS             complement(863..3193)
                     /locus_tag="VFU20_09495"
                     /inference="COORDINATES: protein motif:HMM:NF012786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HEU5286719.1"
                     /translation="MKPSRTSFWSGASLLALLCAGPVAALAPQSDSAEAIAGLRDTLL
                     SVSVNGARTGEPVALLAGPGGTFYASAAVLASWRLATPRPAFSRDGVDYYLLNAIPGL
                     KLEFHQATQSLSLIARPETFERTRLSYAMLEPSDQVVGGTGGFLNYDVSAQVADGDTS
                     LGGAFEAGIFTRFGVGISSFVGRWSNGGAELTRLDSNWTIDDPSRMRSLRLGDGNSRG
                     GAGGVPFRFGGIQLARNFGVQPGFVTIPLPSLRGSAALPSVVDIYVNDALRGSRDVPP
                     GPFEITDVPIVTGNGEVQLVVRDLLGRQMLYSQSYYAAQTLLRKGLHDYSYEAGFLRR
                     SFGERSNDYGAMMVSATHRYGFSDRLTGEIHAEATTGVQSGGVAVGIVLPHVGHAEAS
                     LAASRSGRGTGAQAGLTLERRTRGLSLGARAEVSSKDYTSVGWDRDRRPPASTVQLFA
                     GLPVRFGSLGLSYLRRDARGEPDAEYASASTTVRVARLGSLHLAGRKSLTGDKNLSAA
                     VTWMVPVGPRASATAGAALNRGERTLTSSFQKHLPVGEGIGYRIATSVGTVERIDGRL
                     SAQTGLGTHEAHLTWVDGQAGVRLSTAGGLGLVGGDAFAARRLSQSFAAVKVGDYPNV
                     RVYADNQLVGRTGRDGTMIVPALRPFDRNALRIELADLPWDAEVVGDQLVVRPYDRHG
                     VAVDFQVRTARAAILTILLDDGSALPAGSTVRLDPGPGEFVSAPGGEVYMTGLQSDNS
                     AMASWSSGSCRFQFRFEPGDDPQPRLGRFRCRSIAR"
     gene            complement(3156..3866)
                     /locus_tag="VFU20_09500"
     CDS             complement(3156..3866)
                     /locus_tag="VFU20_09500"
                     /inference="COORDINATES: protein motif:HMM:NF012565.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HEU5286720.1"
                     /translation="MGSAAAIAAWLAFGLALAVPASAGSFKVHPVHINLPAGRQSTSL
                     KLTNSDSAPVSVRVTTLAWTQVDGRDVHTPTNNVIVSPPIFTIPPGKTQIVRIGLRDR
                     RGARAYRVIFEEIPRERTVEGQIQITLRLNLPLYVLAKGRGKADLAWTAWRDGAGEII
                     VEGRNRGSLHGQVVELAADLGGRRHILSNQMGVVLPGSARQWKIGRRSELAMGNPFLL
                     RVKGPAGETQSHIVLERR"
     gene            complement(3915..>4489)
                     /locus_tag="VFU20_09505"
     CDS             complement(3915..>4489)
                     /locus_tag="VFU20_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008830181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="spore coat protein U domain-containing protein"
                     /protein_id="HEU5286721.1"
                     /translation="IAREGHRSAAAADVTPSEKTGVYSMLSSTSRTFRTLVLTGAAIA
                     AVAAQPALAADTAELAVSAEVSEVCNVTTSPVAFGTVDVTSGSNVDATGGISVTCTSG
                     TAWAAAADAGNGSGATVTARKMTSGSDLLNYALYTDSGRTTNFGGANTITGTGTGSAQ
                     ASTVYGRVPSGQTTVPVGSYADSVTISLTY"
BASE COUNT          689 a         1484 c         1643 g          673 t
ORIGIN      
        1 gctgtagggc gtggttcccg agcccttggt gccgaggtcg agcaaccggt cgttttcgtc
       61 gcgcatttgc gcgaacagga agccgcggcc gtcgccgatg tccgggttgt acgccctgaa
      121 ctggtggccg tgatagcgca gcgcgagcgg ctgctcgagg ttatcgggca gcggctcgaa
      181 gcggcagaaa tgcgcctccc attgccgatc gtcgaggttg agtccgacgc gctcggccca
      241 ccgccggttg aggaagcgcg gaatggcctt ggggaaggtc gccggccgga cgacatcgta
      301 gaaatccggg ccgagctcga ggatcttcgg gtcgggccgg taggggactg cggatgtcat
      361 caaggcctta aggtggcggc gcggcagagg ttccacgcaa cgcatgcctg cggcggcaag
      421 tgcgtcatca atcaaaagtc gacggtcacc gcgaccgaat cgacatagac atcggccggg
      481 accgtctggc cggccgggat tcgcccgtag accgtgagct ggacattggt gccgagcgtg
      541 gaaacgccgg ccagcccttc gccccagacg aacagccggg ccgcgtcctt gtagagattg
      601 tagttgagct gtgccgcgcc gccggtcatc cgccggttcg cgaccgtgcc ggtgcccggg
      661 ccgagcgaaa cgacgaagct ggtcggcagg tcgcacgtga cctggacatt gccgaccccg
      721 ttcagggcgg tcgcgctcaa cgggtcgtag gctccgaagt ccacttcttg cgggctgacc
      781 tcgcactcgg ccgaagcggc cgtcgaagcg gcgagcaaca gcaaccccgc cgcggcgcgg
      841 ccgccccatc ggcgaaaagc agtcatcggg cgatgctccg gcaccggaag cggcccagcc
      901 ggggctgtgg gtcgtcgcca ggctcgaagc ggaattggaa gcggcagctg ccgctcgacc
      961 aggatgccat tgcggaattg tcggactgca ggccggtcat atagacttcg cccccgggtg
     1021 cgctgacgaa ctcgcccggc cccgggtcga gccggaccgt cgagccggcc ggcagggccg
     1081 agccgtcgtc gagcaagatg gtgaggatcg cggcgcgggc ggtccggacc tggaaatcga
     1141 ccgcgacccc gtggcggtcg taggggcgga cgacgagctg atcgccgacg acctcggcgt
     1201 cccagggcag gtcggcaagc tcgatccgca aggcgttgcg gtcgaacggc ctcagcgccg
     1261 gcacgatcat cgtcccgtcc cggccggtcc ggccaacgag ctggttgtcg gcatagaccc
     1321 ggacgttggg ataatcgccg accttgacgg ccgcgaagct ctggctcagc cggcgcgcgg
     1381 cgaatgcgtc gccgccgacg aggccgaggc cgcccgcggt cgacagccgg actccggcct
     1441 ggccgtcgac ccaggtcaga tgcgcctcgt gggtgcccaa gccggtctgg gcgctgagcc
     1501 tgccgtcgat gcgctcgacc gttccaaccg acgtcgcgat ccggtagccg atgccctcgc
     1561 cgaccggcag gtgtttctgg aacgagctgg taagcgtgcg ctcgccgcgg ttgagagccg
     1621 cgccggcggt ggcgctcgcg cggggcccga ccggcaccat ccaggtcacg gcggccgaca
     1681 gattcttgtc cccggtgagg ctcttgcggc cggcaaggtg caggctgccc agccgggcga
     1741 cgcggaccgt cgtgctggcg ctcgcatatt cggcgtccgg ctcgccgcgc gcgtcgcggc
     1801 gcaggtagga caggccgagc gagccgaacc gcaccggaag cccggcgaaa agctggacgg
     1861 tcgaggccgg cggccggcga tcgcggtccc agccgaccga cgtgtaatcc ttgctgctga
     1921 cttcggcccg agcgcccagc gacaggccgc gggtccgccg ctcgagggtg agtccggcct
     1981 gcgcgcccgt gccccggccc gaccggctcg cggccagcga cgcctccgcg tggccgacgt
     2041 gcggcagcac gattccgacc gcgactccac cggactgaac gcccgtcgtc gcctcggcgt
     2101 ggatctcgcc cgtcaggcgg tcgctgaacc cgtagcggtg ggtggccgag accatcatcg
     2161 ccccataatc gttgctgcgc tcgccgaacg agcggcgcag gaatcccgcc tcgtaggaat
     2221 aatcgtggag ccctttgcga agcagcgtct gggcggcgta ataggactgg ctgtagagca
     2281 tctggcggcc gagcaggtcg cggacgacga gctggacctc gccgttgccg gtgacgatcg
     2341 ggacgtcggt gatctcgaac ggcccgggtg gcacgtcgcg gctgccgcgc aacgcgtcgt
     2401 tgacatagat atcgaccacc gagggcaggg ccgcgctgcc tcgaagcgac ggcagcggga
     2461 tcgtcacgaa gcccggctgg accccgaaat tgcgggccag ctggatgccg ccgaagcgga
     2521 acgggacccc gccggcgccg cccctcgaat tgccgtcgcc gagccgcagc gagcgcatcc
     2581 gcgacgggtc gtcgatggtc cagttggaat cgagccgggt cagctcggcg ccgccgttcg
     2641 accagcggcc gacgaagctg ctgatgccga ccccgaagcg ggtgaagatc ccggcctcga
     2701 acgccccgcc gagcgacgtg tcgccgtccg cgacctgggc gctgacatcg tagttgagga
     2761 aaccgccggt gccgccgacc acctggtcgc tgggctcgag catcgcgtag gacagccgcg
     2821 tgcgctcgaa cgtctcgggc cgggcgatca gcgacaggct ctgggtcgcc tggtggaact
     2881 cgagcttgag cccggggatc gcgttgagca gataatagtc gacgccgtcg cgggaaaagg
     2941 ccggccgcgg ggtggccagc cgccacgagg caaggactgc ggcgcttgcg tagaatgtgc
     3001 cgccaggccc ggccagcaac gcgaccggtt cgccggtgcg cgctccgttg acgctgaccg
     3061 acagaagcgt gtcccggagg ccggcgatcg cctcggcgct gtcggattgc ggagcgagtg
     3121 ccgcgaccgg gcccgcgcac agcaaggcga gaaggctagc gccgctccag aacgatgtgc
     3181 gactgggttt caccggccgg gcccttcact cgcagcagga acgggtttcc catggcaagc
     3241 tccgagcgcc tgccgatttt ccactgccgg gcgctgcctg ggagaacgac gcccatctgg
     3301 ttggataata tgtgcctccg gccgcccagg tcggcggcga gctcgacgac ctgcccgtgg
     3361 agcgatccgc ggttgcggcc ttcgacgatg atctcgccgg cgccgtcgcg ccacgccgtc
     3421 caggcgaggt cggcctttcc gcggcccttc gccaggacat agagcggaag gttcagccgc
     3481 aaggtgatct ggatctggcc ctcgaccgtc cgttcgcggg ggatctcctc gaagatcacc
     3541 cggtaggcgc gggcgccgcg gcggtcgcgg aggccgatcc gcacgatctg ggtcttgccg
     3601 gggggaatcg tgaagatcgg cggcgagacg atgacgttgt tggtcggggt gtggacatcg
     3661 cggccgtcga cctgggtcca ggccaaggtc gtgacccgca ccgaaaccgg cgcggaatcg
     3721 ctgttggtca gcttcagcga cgtcgactgc cgaccggcgg ggaggttgat gtggaccgga
     3781 tggaccttga acgacccggc ggaagccggg acggcgaggg ccaggccgaa agccagccag
     3841 gcggcaatcg cggcggccga tccgatcgac cggccgccgc gcccgcccct ttccccccgg
     3901 tcgtcatcgg ctctttagta ggtcaggctg atggtgacgg agtcggcgta gctgccgacc
     3961 ggaacggttg tctggccgct cggaacccgg ccgtagacgg tgctcgcctg ggccgaaccg
     4021 gtgccggtgc cggtaatcgt attggcgccg ccgaagttgg tcgtgcgccc gctgtcggtg
     4081 tagagcgcgt agttcagcag gtcggatccg ctggtcatct tgcgggccgt gacagtcgcg
     4141 cccgagccgt tgccggcatc ggccgcggcg gcccaggcgg tgccgctggt gcaggtgacg
     4201 gaaatgccgc cggtggcgtc gacgttgctg ccgctggtga cgtcgacggt gccgaatgca
     4261 accggcgagg tcgtgacgtt gcagacttcg ctgacctcgg cgctgacggc gagttcggcg
     4321 gtatcggcgg cgagcgccgg ctgcgctgca actgcggcga tggcggcgcc ggtcaggacg
     4381 agcgtgcgga acgttcggct tgtcgaactc aacatggaat aaactcctgt tttttcactg
     4441 ggggtaacgt ctgccgccgc ggctgaacgg tgtccttcac gggcaattg
//
LOCUS       DASTZO010000338        10186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_77455,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000338 DASTZO010000000
VERSION     DASTZO010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 10186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10186
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77455"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1068
                     /locus_tag="VFU20_09510"
     CDS             <1..1068
                     /locus_tag="VFU20_09510"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEU5286722.1"
                     /translation="ACKREPGERSHWINLATACRKLDDSERERAALEGALAIDQTDLL
                     ALIRIAELHERLGEETPAAKRWTAVLALASGIDDPSPEFAGILGHAKRYVEDQQRELA
                     DAIDKALADELARASARDGRRIRRAADAWLGRRPIYTNQCEGLHYPFLPADEFFDAEL
                     FPWLGELEAATATIVAELETILADSKAELTPYISLPPGVPASKWSKLDKSLDWGAFHL
                     WKEGERFDEACARAPRTAALVESLPICRIEGRAPNVFFSILKAGSHIPPHTGVTNVRS
                     VVHLPLIVPEGCEFRVGGETRAWVQGRAFAFDDTIEHEAWNRSERDRAVLIVDAWNPY
                     LSDHERAMICRLYGTADVRRR"
     gene            complement(1110..2648)
                     /locus_tag="VFU20_09515"
     CDS             complement(1110..2648)
                     /locus_tag="VFU20_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEU5286723.1"
                     /translation="MKPDPEPLPPSADLAALARGGRINFFGFVLRLAARLPFLFIAGR
                     LYGAEALGRFAYAVLIVEFAAQLATLGLKRGLAQQLAQTDKPHVCVVWDALLVGAVGS
                     VVGMALLFAFPQAMFPNSEIYGMEWLLPTAVIALAWSDVMLAALAYRHDVGATVRARA
                     VVEPWTISAAALGLWFVSPRDGLLFAYALSMLGAVIASAVPFLKSYGVPHGWSPDPAT
                     LWRMARRNLPVAAADAVEWGSRRVDIAILGLFVSPAVVGVYYVAQNIASLPSKLKTSF
                     DPILGPVISRNIAAGNSKAVARQVRQVGFWVIAAQSAVALALAIPGEGVLGLVGPAFV
                     AGTAALAVLLFAEVVAATATVSESALIYCARKRNMAISLAMIVLQALMSVGFILLFKR
                     LGWPEMWQAAGPAIALLLALGMAAVAKARLLSHLLKAPVSGWRWELVWAAAAAAVAGQ
                     LAILLPEWLELSVGIPAILLAFGAVLWWHGFTPEDRELFKMKKSDIEDLSLPDPSTSG
                     DAPR"
     gene            complement(2683..5508)
                     /gene="polA"
                     /locus_tag="VFU20_09520"
     CDS             complement(2683..5508)
                     /gene="polA"
                     /locus_tag="VFU20_09520"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011951298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU5286724.1"
                     /translation="MPDSQGSTANHLYLVDGSSYIFRAYHRLPPLTNREGMNVGAVYG
                     YTTMLWKLADGLNKADGPTHMAVILDKSEHTFRNELYDQYKAHRPPAPEDLVPQFPLI
                     RTATRAFSIPCIEEEGLEANDIIACYVTAAKKAGWRVTIVSSDKDLMQLVEDGSVDML
                     DTMNDRRIDRTAVLEKFGVPPEKVGDVLALMGDSVDNIPGVPGIGPKTASQLIQQFGD
                     LETVLASTGEITKPKLKQSLIDHADAARLSRELVRLVCDAPLPEPLEDLALTGIPPQP
                     LKEFLEDQGFKSLLNRMIGGGGGAVGRSSSGGGINDVMASLASAPAPSAPPSAEAIAV
                     DRSAYETVTDEAALDRWIEEAQANGYVAVDTETDCIDCVVARLAGISIATAPNRACYI
                     PVGHSGADLYSDAPHQLGKALVLAKLKPLLEDGGVLKIGHNLKYDWVMFDKEGIAVAP
                     YDDTMLLSYDLDAGRHNHGMDELAKLHFDHECIAFKTVCGVGAKQITFDKVPLPQATQ
                     YAAEDADITLRLWQRLKPRLAAEGGSQVYERVDKPLVPVVGRMERRGVKVDRDYLAKL
                     SGEFAKEIAALEERIYEAACGPFTIGSTQQLGHVLYERLGLKGGRKGKSGAYSTDVTE
                     LERLAGEGVECARLVLDWRQLTKLKSTYTDALQAQINPETGRVHTSFSLAVAQTGRLA
                     STDPNLQNIPIRTDIGRKIRDAFVAEPGHQLMSADYSQIELRLAAHMADVPALKAAFH
                     AKADIHNLTAEELFGSADRENRNKAKTINFAILYGISSWGLAGRLGVSREEGQAIIDR
                     YFERFPGIRDYIATTLTFARENGFTKTLFGRKTHFPNIASRQQNLRQGSERAAINAPL
                     QGTSADLIKRAMARMDGALEEAGLEGVRMLLQVHDELVFEVPDGREEEAAAVIRRVMA
                     GAAEPAVQLDVPLDVEVGWGRHWGAAH"
     gene            5585..5764
                     /locus_tag="VFU20_09525"
     CDS             5585..5764
                     /locus_tag="VFU20_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286725.1"
                     /translation="MTERFERHRQPWRQDEIQKLHLLAGKGMSLRAIAKALTRSEESV
                     QARAKADKLKISKLR"
     gene            complement(6265..7863)
                     /gene="nadB"
                     /locus_tag="VFU20_09530"
     CDS             complement(6265..7863)
                     /gene="nadB"
                     /locus_tag="VFU20_09530"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011952765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HEU5286726.1"
                     /translation="MSKAQDVLIVGSGAAGLTAALNLAETHTVAVIAKGALGEGATGW
                     AQGGIAAVLEEGDSFEAHVRDTMIAGAGLNDERVVEHVVGAAPQAIERLAQLGVPFAA
                     GTAGERWHLTREGGHSHRRIVHVADATGSAVQRALEAAAAKHPNIRLVPDMVAIDLIT
                     GRHAKAFSTSGAIHGLYAYNRKSKRVEALSARATILATGGAGRAYLYSTAPRGATGDG
                     IAMAWRAGCRVSNMEFMQFHPTCLYNLQVKNFLITEALRGEGAQLKLPPGVKGGGTRF
                     MARFDERAELAPRDIVARAIDHEIKRLGLDYVHLDISHRDADFVKGHFPNIYERLLGL
                     GIDITAEPIPVVPAQHYTCGGVVVDLDGRTDAPGLYAAGEVTQSGLHGANRLASNSLL
                     ECLVFGEAAARHIAANWDELPQPPAIRAWDESRVTDSDEEVVIQQIWGEIRRFMWNFV
                     GIVRTTKRLERAKRRIDMLRQEVGEYYRNFRVTPDLIELSNLLEVADLIVRSALSRHE
                     SRGLHYTLDYPEMAAEAVDTVLVP"
     gene            complement(7860..8804)
                     /locus_tag="VFU20_09535"
     CDS             complement(7860..8804)
                     /locus_tag="VFU20_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5286727.1"
                     /translation="MSAAEPAIRIESLSKTYASKGAPPKLALDDVSFGVPRGQIFGLL
                     GPNGAGKSTLINILAGLVTKSKGRVSIWGFDIDQHPRNAKRSIGIVPQEILFDPFFSP
                     REALEIQAGLYGIPPSQRRSDDLLAAMHLSDKADAYARTLSGGMKRRLLVAKAMVHSP
                     PILVLDEPTAGVDIELRKELWDYVRSLNRQGVTVVLTTHYLEEAEQLCDRIAIIHRGK
                     VVANEPTRELIAKAQEKSVVVTFDKDIASVPTAPCFENIGLLNERTLEITYRKDRVNA
                     GEVLAALAADGLAIVDVSTRDPDLEDVFLSLVAEDSAA"
     gene            8916..9449
                     /locus_tag="VFU20_09540"
     CDS             8916..9449
                     /locus_tag="VFU20_09540"
                     /inference="COORDINATES: protein motif:HMM:NF025710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HEU5286728.1"
                     /translation="MIGDFHLRGLLMGLAALVAQFAAAPLAHAAPVAPDRNATATVQI
                     APPASLRKLEDLNFAYLSVTTAGTAIVDPNTDSMTTTGGVIHAGGVPYAALFEAVSPV
                     KAVVIIRIPRNPITVTRVGGTETMTVSNWTLSGNSKRTVAAQEAFSFKVGGTLTVNAN
                     QVEGLYRGTFTVEVQYP"
     gene            9516..9716
                     /locus_tag="VFU20_09545"
     CDS             9516..9716
                     /locus_tag="VFU20_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013847861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HEU5286729.1"
                     /translation="MPIPPPETIRVKTVQVACDGSGDIPAALGHPRVFLRIEPETGFV
                     ECGYCDRRFVLEGGPADQPQQS"
     gene            9713..>10186
                     /locus_tag="VFU20_09550"
     CDS             9713..>10186
                     /locus_tag="VFU20_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HEU5286730.1"
                     /translation="MTGDPRRFLYRDGMLDPDEAKRLAARHLAACDDGELYLQYTASE
                     SFGFDDGRLKTADYHSSSGFGLRGVAGETTAFAHANEISAAAIERAAATLRLLDPANA
                     AAAPSPRRTNQSMYGADDPLSAIPFAQKVALCQQIDAAARARDPRVSQVSVSLAGS"
BASE COUNT         1589 a         3495 c         3464 g         1638 t
ORIGIN      
        1 gcctgcaaac gcgagcccgg cgagcggtcg cactggatca atctggcgac ggcctgtcgg
       61 aaactcgacg attccgagcg cgagcgagcg gctctcgaag gagcgctcgc catcgatcaa
      121 accgacctgc tggcgctgat tcgaattgcc gagcttcacg aacggctcgg ggaggagacg
      181 ccggcggcca agcgctggac ggccgtcctc gcgctcgcgt cgggcatcga tgacccgtcg
      241 cccgaattcg ccggcatcct tggtcatgcg aagcgatatg tcgaagatca gcaacgggag
      301 cttgcggacg ccatcgacaa agcgctggcc gacgaattgg ccagggccag tgcccgggac
      361 gggcggcgaa ttcgcagggc cgccgacgcc tggctcggaa ggcggccaat ctacacgaac
      421 caatgcgaag gcctgcatta cccattcctg cccgccgacg aattcttcga cgccgagctt
      481 tttccctggc tcggcgagct cgaggccgcc accgcgacca tcgtggccga gctggagacg
      541 atcctcgcgg actcaaaggc cgagctgacg ccctatattt cgctgccgcc cggggtgccg
      601 gcgagcaaat ggtcaaaact ggacaaatcg ctggactggg gcgccttcca cctgtggaag
      661 gagggcgagc gcttcgatga ggcttgtgcc cgcgcgccgc ggaccgcggc cctggtggaa
      721 tcgcttccga tctgccgcat cgaaggccgg gcgccgaacg tctttttctc gatcctgaag
      781 gccggcagcc atatcccgcc gcacacgggg gtgaccaatg tgcgcagcgt cgtccacttg
      841 cccttgatcg ttcccgaggg atgcgagttc cgtgtcggcg gtgagacacg cgcctgggtc
      901 cagggccggg cgttcgcctt cgacgacacg atcgagcatg aggcatggaa ccgcagcgag
      961 cgcgaccgcg cggtgctgat cgtcgacgct tggaacccct atctcagcga ccatgagcgg
     1021 gccatgatct gccgcctgta cggcacggcc gacgtgcgcc gccgctaagc ggcagccgct
     1081 gccgcccacg cgtcattttg gagcggcgtt cagcgcggcg cgtcgccgct tgtgctcggg
     1141 tcgggcaggc tcaaatcctc gatgtccgac ttcttcatct tgaacaactc gcggtcctcg
     1201 ggcgtgaagc cgtgccacca cagcaccgct ccgaacgcca gcaggatcgc gggaatgccg
     1261 acgctgagtt cgagccattc gggaagcagg atcgcaagct gcccggcgac cgcggccgcg
     1321 gccgccgccc acaccagctc ccaccgccag ccggacaccg gcgctttcag caaatgcgac
     1381 agcagccgcg ccttggcgac cgcggccatt ccgagcgcca gcaatagcgc gatcgccggc
     1441 cccgccgcct gccacatctc gggccagccc aaccgcttga acagcaggat gaagccgacc
     1501 gacatcagcg cctggagcac gatcatcgcc aggctgatcg ccatgttgcg cttgcgcgcg
     1561 caatagatca gcgccgattc gctgaccgtc gcggtcgccg cgacgacctc ggcgaacagc
     1621 agcacggcga gcgccgcggt gccggccacg aacgccggcc cgaccagccc gagcacgccc
     1681 tcgcccggga tcgccagcgc cagagcgacc gcgctttgcg ccgcgatcac ccagaagccg
     1741 acctggcgga cctgcctggc gaccgccttg ctgttgcccg cggcgatgtt gcgcgagatc
     1801 accggcccca ggatcgggtc gaagctggtc ttcaatttgg acggcagcga ggcgatgttc
     1861 tgggcgacgt aatagacgcc gaccaccgcc gggctgacga acagtccgag gatggcgatg
     1921 tcgacccggc gcgatcccca ttcgaccgcg tcggcggcgg cgaccggcag gttgcgccgg
     1981 gccatccgcc acagggtcgc cgggtcgggg ctccagccgt gcggcacccc gtagctcttc
     2041 aaaaaaggaa cggccgacgc gatcaccgcc ccgagcatcg acagcgcata ggcgaacagc
     2101 agcccgtcgc gcggcgacac gaaccacagg cccagcgccg cggcgctgat cgtccacggc
     2161 tcgaccaccg cgcgggcgcg cacggttgcg ccgacgtcgt ggcgataggc gagcgcggcg
     2221 agcatcacgt ccgaccaggc gagcgcgatc accgcggtcg gaagcagcca ctccatcccg
     2281 tagatttcgc tgttggggaa catcgcctgc gggaaggcga acagcagggc catgccgacc
     2341 actgatccga ccgccccgac cagcagcgcg tcccagacga cgcagacgtg cggcttgtcg
     2401 gtttgggcga gctgctgcgc gagcccgcgc ttgaggccga gcgtcgccag ctgggcggcg
     2461 aactcgacga tcagcaccgc ataagcgaaa cgcccgagcg cctcggcgcc gtacagccgg
     2521 ccggcgatga acaggaacgg cagccgcgcc gccagtcgaa gcacgaagcc gaagaagttg
     2581 atccggccgc cgcgggcgag cgcggccagg tccgccgacg gcggcagcgg ctcggggtcc
     2641 ggcttcatcg ctgcgcgacg ctttttcaat gcccgccgcc cgtcaatgtg cggcgcccca
     2701 gtggcggccc cagccgacct cgacgtccag cggcacgtcg agctggaccg ccggctccgc
     2761 ggcccccgcc atcacccgtc ggatcacggc ggcggcctcc tcctcgcgcc cgtcggggac
     2821 ctcgaacacc agttcgtcgt ggacctggag cagcatccgc accccctcga gcccggcctc
     2881 ctcgagcgcc ccgtccatcc gcgccatcgc ccgcttgatc agatcggcgc tggtcccctg
     2941 gagcggcgcg ttgatcgccg cccgctcgct gccctggcgc aaattctgct ggcggctggc
     3001 gatgttgggg aagtgggtct tgcggccgaa cagggtcttg gtgaagccgt tctcgcgggc
     3061 gaaggtcagg gtcgtggcga tatagtcgcg gattcccggg aagcgctcga aatagcggtc
     3121 gatgatcgcc tggccttcct cgcggctgac cccgagccgc ccggccaggc cccaggacga
     3181 aatgccgtac aggatcgcga agttgatcgt cttggccttg ttgcggttct cgcggtcggc
     3241 cgagccgaac agttcctcgg cggtcaggtt gtggatgtcg gccttggcgt ggaacgcggc
     3301 cttgagcgcc ggcacgtcgg ccatgtgcgc ggcgaggcgc aattcgatct ggctgtagtc
     3361 ggcgctcatc agctggtggc cgggctcggc gacgaaggcg tcgcggatct tgcggccgat
     3421 gtcggtgcgg atcgggatgt tctggaggtt ggggtcggtc gacgccagcc gcccggtctg
     3481 ggcgacggcg aggctgaagc tggtgtggac gcggccggtc tcggggttga tctgcgcctg
     3541 caatgcgtcg gtgtaggtgc tcttcaactt ggtcagctgg cgccagtcga gcaccagccg
     3601 cgcgcattcg acgccctcgc ccgccagccg ctcgagctcg gtgacgtcgg tcgagtaggc
     3661 gccgctcttg cccttgcgcc cgcccttgag gccgagccgc tcgtagagca catggcccaa
     3721 ttgctgggtc gagccgatgg tgaacggccc gcacgccgct tcgtagatgc gctcctcgag
     3781 cgccgcgatc tctttggcga actcgcccga cagcttggcg agatagtcgc ggtcgacctt
     3841 gaccccgcgc cgctccatcc gcccgaccac cggcaccagc ggcttgtcga cccgctcgta
     3901 gacctgggac ccgccctcgg cggcgaggcg cggcttgagc cgctgccaca gccgcagcgt
     3961 gatgtcggcg tcctcggccg catattgtgt cgcctgcggc agcggcacct tgtcgaaggt
     4021 gatctgcttg gcgccgaccc cgcacaccgt cttgaaggcg atgcactcat ggtcgaagtg
     4081 cagcttggcc agctcgtcca tgccgtggtt gtggcgcccg gcgtcgaggt cgtagctcag
     4141 gagcatcgtg tcgtcgtacg gcgcgacggc gatcccctcc ttgtcgaaca tcacccagtc
     4201 gtacttgaga ttgtgcccga tcttgagcac gcccccatcc tcgagcagcg gcttcaattt
     4261 cgccagcacc agcgccttgc cgagctggtg cggagcgtcg gaatataggt cggcgccgct
     4321 gtgcccgacg gggatgtagc aggcgcggtt gggggcggtc gcgatgctga tcccggcgag
     4381 ccgggcgacg acgcaatcga tgcagtcggt ctcggtgtcg acggcgacat agccgttcgc
     4441 ttgcgcctcc tcgatccagc ggtcgagcgc ggcttcgtcg gtcaccgtct cataggccga
     4501 gcggtcgacc gcgatcgcct cggccgaggg cggagcagaa ggggcgggag ccgaggcgag
     4561 cgacgccatg acgtcattga tcccgccgcc gctgctgctc cgtccgaccg ctccgccgcc
     4621 gccgccaatc atccggttca agagcgactt gaacccctgg tcctcgagga actccttgag
     4681 gggctgcggc gggattccgg tcagcgcgag gtcctcgagt ggctcgggca gcggcgcgtc
     4741 gcacaccagc cgcaccagtt cgcgcgacaa tcgcgccgcg tcggcatggt cgatcagcga
     4801 ctgcttcaat ttgggcttgg tgatttcccc ggtgctggcg agcaccgtct cgaggtcgcc
     4861 gaactgctgg atcagctgcg aggcggtctt cgggccgatg ccgggcactc cggggatgtt
     4921 gtcgacgctg tcgcccatca gcgccagcac gtcgccgacc ttctccggcg gcaccccgaa
     4981 cttctcgagc accgcggtgc ggtcgatccg ccggtcgttc atcgtgtcga gcatgtcgac
     5041 cgagccgtcc tcgaccagct gcatcagatc cttgtcggag ctgacgatgg tcacccgcca
     5101 gccggccttt ttcgccgcgg tcacgtagca ggcgatgatg tcgttggcct cgagcccttc
     5161 ctcctcgatg cacgggatcg agaatgcccg ggtcgcggtc cggatcagcg ggaattgcgg
     5221 gaccaggtcc tcgggcgcgg gcgggcggtg cgccttgtac tggtcgtaca gctcgtttcg
     5281 aaacgtgtgc tcggacttgt cgaggatcac cgccatgtgg gtcggcccgt cggccttgtt
     5341 gaggccgtcg gcgagcttcc acagcatggt cgtgtagccg tagaccgcgc cgacgttcat
     5401 gccttcccgg ttggtcagcg gcggcagccg gtggtaggcg cggaaaatgt agctcgaccc
     5461 gtcgacgagg tagaggtggt tggcggtgga accctgggag tccggcatga acggcggggt
     5521 ttagcagggt gagcgcgtga ttccagccgc ggccgcccgc cggaaaggga tggggaagac
     5581 agggatgacc gaacgcttcg agcgccaccg ccagccgtgg cgccaggacg agatccagaa
     5641 gctccatctg ctggccggca agggcatgag cctgcgcgcc atcgccaagg cgctgacccg
     5701 gagcgaggaa tcggtccagg cccgcgccaa ggccgacaag ctcaagatca gcaagctgcg
     5761 gtgagtcttt gaccgtgtgg acgttcgcga tcctggcatc cttgatgatc gatcctgtcg
     5821 cggcggcaag ccccgacagc atcaccggcg agtggaaaaa ccggactcag acggttgcgg
     5881 tccaaatcct tccatgcggc gtcgaacttt gcgggcacgt gacttgggcc tcgcaatccg
     5941 cccaggaaga ttccgcgcgg gccgggacgg agcacctggt cgggacgcag gttctttacg
     6001 gccttaggcc cgccggtccc gaccggtggc gaggcatgct gttcataccc gacctagggc
     6061 ggaggacccg cgccagcgtc gtcaggaagg gccccgacaa gctccaggtg atcggttgcg
     6121 agtcacttgg tttgatttgc aggaagcaga cctggagtcg cgcacgagag gccgaagcag
     6181 tcgcccgcga ggcggactga cctgctcggc gcaggcgagg cagaaggtcg cgcgtcgatt
     6241 gtcgccattt tgtcgaaggt gccgctacgg caccagcacc gtatccaccg cctccgccgc
     6301 catttcggga taatcgagcg tgtagtggag gccgcggctt tcgtggcggg acagcgccga
     6361 gcggacgatc aaatcggcga cctcgagcag gttgctcagc tcgatgaggt cgggcgtcac
     6421 ccggaagttg cggtaatatt cgccgacttc ctggcgcagc atgtcgatcc gccgcttggc
     6481 gcgctccagc cgcttggtcg tccggacgat gccgacgaaa ttccacatga agcggcggat
     6541 ctcgccccag atctgctgga tcacgacctc ctcgtcgctg tcggtgaccc ggctttcgtc
     6601 ccacgcccgg atcgccggcg gctggggaag ctcgtcccaa ttggccgcga tgtggcgagc
     6661 cgcggcctcg ccgaacacca ggcattcgag caggctgttc gacgcgaggc ggttggcgcc
     6721 gtgcaagccc gactgggtga cctcgccggc cgcgtagagc cccggcgcgt cggtgcgccc
     6781 gtcgaggtcg accaccaccc cgccgcacgt gtaatgctgc gccgggacca ccgggatcgg
     6841 ctcggcggtg atgtcgatgc cgaggccgag cagccgctcg tagatattgg ggaaatggcc
     6901 cttcacgaaa tcggcgtcgc ggtgcgagat gtcgaggtgg acatagtcga gcccgagccg
     6961 cttgatctca tggtcgatgg cgcgcgcgac gatgtcccgc ggagcgagct cggcgcgctc
     7021 gtcgaaccgc gccatgaacc gtgtgccgcc gcccttgacg ccgggcggca gcttcagttg
     7081 cgcgccctcg ccgcgcagcg cctcggtgat caggaagttc ttgacctgca aattgtacag
     7141 gcaggtcggg tggaactgca tgaactccat gttcgacacc cggcagccgg cccgccacgc
     7201 catggcgatg ccgtcgccgg tcgccccgcg cggagccgtc gaatataaat aagcgcgacc
     7261 ggcgccgccg gtcgcgagga tggttgcccg ggcgctcagc gcctccacgc gcttcgactt
     7321 gcggttgtac gcgtagagcc cgtggatcgc gccgctggtc gagaaggcct tcgcatggcg
     7381 gccggtgatc aggtcgatcg ccaccatgtc gggcaccagg cggatgttcg gatgcttggc
     7441 ggcggcggcc tcgagcgccc gctgcaccgc cgagccggtc gcgtcggcga catggacgat
     7501 gcggcggtgg ctgtggcccc cctcgcgagt caggtgccag cgctcgcccg cggtcccggc
     7561 cgcgaacggc acgcccagtt gcgccagccg ctcgatcgcc tgcggggcgg cgccgacgac
     7621 atgctcgaca acgcgctcgt cgttgaggcc ggccccggcg atcatcgtgt cgcggacgtg
     7681 cgcctcgaag ctgtcgccct cctcgagcac cgcggcgatg ccgccctgcg cccagccggt
     7741 cgcgccttcg cctagcgccc ccttggcgat caccgcgacc gtgtgcgtct cggcgaggtt
     7801 gagcgccgcg gtcagcccgg ccgcccccga gccgacgatc agcacgtcct gcgccttgct
     7861 catgccgccg agtcttcggc gaccaggctc aggaacacgt cctcgaggtc ggggtccctg
     7921 gtcgagacgt cgacgatcgc gaggccgtcg gcggcgagcg cggcgagcac ctcgccggcg
     7981 ttgacccggt ccttgcggta ggtgatctcg agcgttcgct cgttgagcag gccgatattc
     8041 tcgaagcagg gcgcggtcgg caccgaagcg atgtccttgt cgaaggtgac gacgaccgat
     8101 ttctcctgcg ccttggcgat cagctcgcgc gtcggctcgt tggcgaccac cttgccgcgg
     8161 tggatgatcg cgatccggtc gcacaattgc tcggcttcct cgaggtaatg ggtggtcagc
     8221 accaccgtca cgccctggcg attcaacgag cggacataat cccacagctc cttgcgaagc
     8281 tcgatgtcga ccccggccgt cggctcgtcg agcaccagga tcggcggcga gtggaccatc
     8341 gccttggcga ccagcagccg ccgcttcatg cccccggaca gggtgcgcgc ataggcgtcg
     8401 gccttgtcgc tcagatgcat cgccgcgagc aggtcgtcgc tgcgccgctg cgagggcggg
     8461 attccgtaca gcccggcctg gatctcgagc gcttcgcggg gcgagaagaa cgggtcgaac
     8521 aggatttcct gggggacgat gccgatcgag cgcttggcgt tgcgcgggtg ctggtcaatg
     8581 tcgaagcccc agatcgacac ccgccctttg ctcttcgtta ccagcccggc aaggatattg
     8641 attagagtcg acttgccggc gccgttgggg cccagcaggc cgaagatctg cccgcgcggc
     8701 actccgaagc tgacgtcgtc gagggcgagc ttgggcggag cgcccttgga ggcataggtt
     8761 ttcgacagcg attcgatgcg gatcgcgggc tcggcggcgc tcattgcgac cgcataggca
     8821 ccggcccaac aagcgacaag ggagcgcccg gtaaacgcgc cgtttaccgt gtccgctaga
     8881 ctgatcttga agttaacggc ctatcgcgct ggccaatgat cggtgatttc cacctccgcg
     8941 gcctgcttat gggtctcgcg gcgcttgtcg cgcaattcgc cgcggctccg ctcgcgcatg
     9001 ccgctccggt ggcgcccgac aggaacgcga ccgcgaccgt gcaaatcgcg ccgccggcca
     9061 gcctgcgcaa gctcgaggac ctcaatttcg cgtatctgtc ggtgaccacc gcgggcaccg
     9121 cgatcgtcga cccgaacacc gattcgatga ccaccaccgg cggagtgatc catgccggcg
     9181 gagtccccta tgcggcgctg ttcgaggccg tgtcgccggt caaggcggtg gtgatcatcc
     9241 gcatcccgcg caacccgatc accgtcaccc gcgtcggcgg cacggaaacg atgacggtgt
     9301 cgaactggac gctgtccggg aattcgaagc ggacggtcgc cgcccaggag gccttttcgt
     9361 tcaaggtcgg cggcaccctc accgtcaacg ccaaccaggt cgagggactc taccgcggca
     9421 ctttcacggt cgaggtccaa tatccgtaac gcagccgggc gtcgttgaag cgcgccctca
     9481 ttgaagcgac ggggagcggt tgctagggcg ggcggatgcc catcccgccg cccgagacca
     9541 tccgagtgaa gaccgtccag gtcgcctgcg acggctcggg ggacatcccg gcggcgctcg
     9601 gccatccgcg cgtgttcctt cgaatcgagc ccgagaccgg cttcgtcgag tgcggctatt
     9661 gcgaccgccg cttcgtcctc gagggcgggc cggccgacca gccgcagcag tcatgacggg
     9721 tgatccgcgg cgcttcctgt accgcgacgg gatgctcgat ccggacgagg cgaagcggct
     9781 cgcggctcgg cacctggcgg cctgcgacga cggcgagctc tacctccagt acaccgcttc
     9841 ggagagcttc ggcttcgacg acggccggct gaagaccgcc gactaccaca gctcgtcggg
     9901 cttcggattg cgcggcgtgg cgggcgagac caccgccttc gcccacgcca acgagatcag
     9961 cgccgcggcg atcgagcgcg ccgccgcgac cctgcgattg ctcgaccccg cgaacgccgc
    10021 cgccgctccc tcgccccggc gcaccaacca gtcgatgtac ggcgccgacg acccgctgtc
    10081 ggcgatcccg ttcgcgcaga aggtggcgct gtgccagcag atcgacgccg cggcgcgcgc
    10141 gcgtgacccg cgcgtttcgc aggtcagcgt gtcgctcgcc ggcagc
//
LOCUS       DASTZO010000339         3709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_337749,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000339 DASTZO010000000
VERSION     DASTZO010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 3709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3709
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337749"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..1012)
                     /locus_tag="VFU20_09555"
     CDS             complement(53..1012)
                     /locus_tag="VFU20_09555"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HEU5286731.1"
                     /translation="MALPPILSNLRLPVIGAPLFIVSSPALVIAQCTSGIVGSFPALN
                     ARPAEKLDEWLHAITEALAAHDEAHPDRPAAPFAVNQIVHRSNDRFERDMEVCAKWKV
                     PIVITSLGARTELNDAVHGWGGITLHDVIDDRFARKAVEKGADGLIAVAAGAGGHAGR
                     WSPFALVHEIRRWFDGPLVLSGAIATGGAILAAQAMGADLAYIGSPFIATPEANSSDA
                     YKQGIVEASAADIVYTDLFTGVHGNYLRSSILASGLDPDALAAGSIDAMKFGSDGSSK
                     SKAWRDIWGSGQGVGAVDRILPAAELVAKLAEEYAAAKARVCG"
     gene            1092..2441
                     /locus_tag="VFU20_09560"
     CDS             1092..2441
                     /locus_tag="VFU20_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5286732.1"
                     /translation="MPAAATLATPPPQAGQLLRVLGLAFALAVGVGTVVGGGILRTPA
                     VVFDAVPHAGIALALWVAVGLHCLLQANVISELMTALPRAGGLFVPARAAFGEPGGLL
                     VGWTDWLGYIAAIAALAILASDFTVMAVPALTPYVSHLAVAFLAAFVAVNWLGIREGS
                     TTQIVGSAAKALFLFGLVALIFLSRPADPSALERFAGPVGLVGLIVAYQTIFGAYTGF
                     SNVAYFAEEDRDPGRNIPRGMMMTVLTVIALYVLVSAALYLTMNPAALAKSTLPVADA
                     LAPVLGPFALSLIAGGAVLIALTCLNANLMGAPRILYGLSEQRMFPAAALRVNRGGTP
                     TVAMGLSAAAALGLTLTGQFETVFLIMAALSLVPFVVADLALFKLRRDAPELPRPYRA
                     KLYPWLPALALLLDFGMLIAFLAADWTSGLFIVAAIAICVPIGLWMRRNQSEPILLG"
     gene            complement(2448..3518)
                     /locus_tag="VFU20_09565"
     CDS             complement(2448..3518)
                     /locus_tag="VFU20_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011541047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEU5286733.1"
                     /translation="MHRFPLKLIAVAALALSSSCATPLLRAPSAGTYANPVLDSDFPD
                     PVVLKAPDGYYYAYATQTQIGGKWVNIQLARSPDLVNWQHLGDALPAKPTWASKTQDF
                     WAPHVARHGDTYYMYYSAKPDTADERHGLCLAVATAGGPLGPFVDIGKPLQCGEGFVN
                     IDPMSFDDPATGKRLLYWGSGFQPIKVQELGPDRLSFAPGSKPIDLIPAIKDDPKRYS
                     RLVEGAWVIRRGDYYYLFYSGDNCCGKDAHYAVLVARSKSATGPFETLAQATGAPTSA
                     ILEQRGIWFAPGHNATITDAAGVEWMVYHAVDVRRARTKPTDDLNTRRIMMIDPIAWR
                     DGWPRIENNGPSTERKRAPKVR"
BASE COUNT          503 a         1274 c         1298 g          634 t
ORIGIN      
        1 cggcccgagc gctaatgggg cggctcgagc gcggcctcgg cgagcaattg cactaaccgc
       61 aaacgcgcgc cttcgcggcc gcatattcct ccgcaagctt cgcgaccagc tccgcggccg
      121 gcaggatccg gtcgaccgcc ccgactccct ggcccgatcc ccagatgtcg cgccacgcct
      181 tggacttgct cgacccgtcc gaaccgaact tcatcgcgtc gatgctgccg gcggcgagcg
      241 cgtcggggtc gagccccgag gcgaggatcg acgagcgcag gtaattgccg tgaacgccgg
      301 tgaacaggtc ggtgtagacg atgtcggcgg cgctcgcctc gacgatgccc tgcttgtacg
      361 cgtccgagct gttggcctcg ggcgtggcga tgaacggcga gccgatgtag gcgaggtcgg
      421 cgcccatcgc ctgcgcggcg aggatcgccc cgccggtcgc gatcgctccc gacagcacca
      481 gcggcccgtc gaaccagcgg cggatctcgt ggaccagagc gaacggcgac cagcgaccgg
      541 catggccgcc tgcaccggcc gcgaccgcga tcaggccgtc ggcgcctttt tcgaccgctt
      601 tcctcgcgaa gcggtcgtcg atcacgtcgt ggagcgtgat cccgccccag ccatggaccg
      661 catcgttcaa ttcggtgcgc gcgcccagcg aggtgatgac gatcggcacc ttccacttcg
      721 cgcacacttc catgtcgcgc tcgaagcggt cgttggagcg gtggacgatc tggttgaccg
      781 cgaacggcgc ggcgggacgg tcgggatgcg cttcgtcgtg cgccgccagc gcctcggtga
      841 ttgcgtgcag ccattcgtcg agcttctcgg cgggccgggc gttcaaggcc gggaagctgc
      901 cgacgatccc cgacgtgcac tgggcgatca ccagggctgg gctggagacg atgaacaagg
      961 gcgcgccgat caccggcagt cgcaggttcg acaggatcgg cggcagtgcc atggccgcgc
     1021 ttagccgcat ccggtcgccg ctgcctagac gcctgctgga caggccgcga ttatcggggc
     1081 agcgtccgtc catgccagct gccgccacgc tcgccacccc gccgccgcaa gcgggacagc
     1141 tgctgcgcgt gctcgggctg gcgttcgcgc tcgcggtcgg ggtcgggacg gtggtcggcg
     1201 gcggcatcct gcgcacccca gcggtggtgt tcgatgccgt cccccacgcc ggcatcgcgc
     1261 ttgccctgtg ggtcgccgtc gggctgcact gcctgctcca ggccaacgtg atttccgagc
     1321 tgatgacggc tttgccgcgc gccggcggcc tgttcgttcc ggcgcgggcc gcgttcggcg
     1381 agccgggcgg gctcttggtc ggctggacgg actggctcgg ctacatcgcg gccatcgcgg
     1441 cgctggcgat cctcgcctcc gatttcacgg tcatggcggt cccggcgctg acgccctacg
     1501 tcagccatct cgcggtcgcc ttcctggccg cgttcgtggc ggtcaactgg ctcgggatcc
     1561 gcgaaggaag cacgacccag atcgtcggca gcgcggccaa ggccctgttc ctgttcgggc
     1621 tggtcgcact gatcttcctg tcgcgcccgg ccgatccttc cgctctcgag cgcttcgccg
     1681 gcccggtcgg gctggtcggg ttgatcgtcg cctaccaaac catctttggc gcctacaccg
     1741 gcttttccaa cgtcgcttat ttcgccgagg aggaccgcga cccgggccgc aacatccccc
     1801 gcgggatgat gatgaccgtg ctgaccgtga tcgcgcttta tgtgctggtc agcgcggcgc
     1861 tgtacctgac catgaacccc gcggcgctgg cgaaatcgac cttgccggtc gccgatgcgc
     1921 tcgcgccggt gctcgggccg ttcgctctca gcctgatcgc cggcggcgcg gtgctgatcg
     1981 cgctgacctg cctcaacgcc aatctgatgg gcgcgccgcg catcctttac ggcctttccg
     2041 agcagaggat gttcccggcc gccgccttgc gggtcaaccg cggcggcact ccgaccgtgg
     2101 cgatggggct gagcgctgcg gcggcgctcg gcctgaccct gaccggccag ttcgagacgg
     2161 tgttcctgat catggcggcg ctcagcctgg tgccgttcgt cgtcgccgac ctcgcactgt
     2221 tcaagctgcg ccgcgacgcg ccggagctgc cgaggccgta ccgggcgaag ctctacccgt
     2281 ggcttcccgc gctggcgctg ctgctcgact tcgggatgct gattgcgttc ctcgccgccg
     2341 actggaccag cggactgttc atcgtcgcgg cgatcgccat ctgcgtcccg atcggcctgt
     2401 ggatgcggcg caaccagtcc gagccgatcc ttctcggctg aggcgggtca gcggaccttc
     2461 ggcgcccgct tgcgctctgt cgacgggccg ttgttctcga tccgcggcca gccgtcgcgc
     2521 caggcgatcg ggtcgatcat catgatccgg cgggtgttga gatcgtccgt cggcttggtc
     2581 cgcgcccgcc gcacgtcgac cgcgtgatag accatccatt cgactccggc ggcgtcggtg
     2641 atggtggcat tgtgaccggg ggcgaaccag atcccgcgct gctcgaggat cgcgctggtc
     2701 ggcgcgccgg tcgcctgggc gagcgtttcg aacggcccgg tcgcgctttt cgatcgcgcg
     2761 acgaggaccg cgtaatgcgc gtccttcccg cagcaattgt cgcccgagta gaacagataa
     2821 taatagtcgc cgcgccggat cacccaggcg ccctcgacca gccgcgaata gcgcttcggg
     2881 tcgtccttga tcgccgggat caggtcgatg ggcttgctcc ccggagcgaa ggacaggcgg
     2941 tcggggccga gttcctggac cttgatcggc tggaagcccg agccccagta gagcagtcgt
     3001 ttgccggtcg ccgggtcgtc gaacgacatc gggtcgatgt tgacgaagcc ctcgccgcac
     3061 tggagcggct tgccgatgtc gacgaacggg cccaacgggc cgccggccgt cgccacggcc
     3121 aggcacaggc cgtgccgctc gtcggcggtg tcgggcttgg ccgaatagta catgtaatag
     3181 gtgtcgccgt gccgggcgac gtgcggcgcc cagaaatcct gggtcttcga cgcccaggtc
     3241 ggcttggcgg gaagagcgtc gccgaggtgc tgccagttaa ccaggtcggg cgagcgggcg
     3301 agctgtatgt tgacccactt gccgccgatc tgggtctggg tcgcgtaggc ataataatag
     3361 ccgtcgggcg ccttcagcac gaccgggtcg gggaagtccg aatcgagcac cgggttggcg
     3421 taggtgccgg cgctcggagc gcgcagcagc ggagtcgcgc agctcgacga caaggccagc
     3481 gcggcgacgg cgatgagttt gagcgggaaa cgatgcacgg ccgcgaccat aagctgggtc
     3541 gcgccatgcc gaaaggggtt ctcatcggcg ggccgcgcac ctatcgcgcg cgccgatggc
     3601 gatgtctttc cacgtcgatg ccgccgagga aggcgagggg gtgcgcttcg ccgtgcgcgc
     3661 gtccggtggc gccgccaaag gctatgccgt cgccgccagc gagcagccg
//
LOCUS       DASTZO010000340         8097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_835081,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000340 DASTZO010000000
VERSION     DASTZO010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 8097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8097
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_835081"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="VFU20_09570"
     CDS             <1..953
                     /locus_tag="VFU20_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003040357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEU5286734.1"
                     /translation="TPVEALLKAYQEKLQSVEKERVDHYAGLREAVELVRTGQGQVRD
                     ETRNLVNALRSSPKARGRWGEQSLKNVLEQSGLSPYADFRTEVSVDGEDGKLRPDVVV
                     RLPGGRQLIIDAKCSLNAYLDACDEVDEDKRQACFRAHVASIRNHAQQLGSKAYWAQF
                     GDSADYVVMYIPGEHFLTAALEQDDGLWDWAFERRVLLATPTNLVAIARTVASVWRQE
                     RLTEAAEEIANLGKELHSRLSTMSQHMEKVGKNLATANTAYNQMVGSFESQVLTQARR
                     FETLGAGSAKELDTPPMVEASPRPLTKIAARASDEEVAAE"
     gene            complement(958..1377)
                     /locus_tag="VFU20_09575"
     CDS             complement(958..1377)
                     /locus_tag="VFU20_09575"
                     /inference="COORDINATES: protein motif:HMM:NF015669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HEU5286735.1"
                     /translation="MRRSAMAVALGLAGALQACAVTPTAPAPITDLAGTAWRVVAVNS
                     RPTSAAGDYSMRFDTSSGIGARFGCNSMGGQYRLAGDLLTVGNLATTLMGCPEPAGTL
                     EREGSAVLGQPMRIAFSSNERMYLSNAAGSIALDPAP"
     gene            complement(1381..1734)
                     /locus_tag="VFU20_09580"
     CDS             complement(1381..1734)
                     /locus_tag="VFU20_09580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286736.1"
                     /translation="MNRLLSLAPPALLAALVTGCITPPPAPAAPYHARGTQPDWSLII
                     DEQHVTFIRADQPVFREPTPVPAITAAGTTYQSPRVRVSIVHALCRDGESDRVYPDRV
                     RVDVDGRAFHGCGGL"
     gene            complement(1731..2561)
                     /locus_tag="VFU20_09585"
     CDS             complement(1731..2561)
                     /locus_tag="VFU20_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010546172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU5286737.1"
                     /translation="MPRLITAFSNSTVKRLRSLRDKKARRSEGLFLAEGLRIVAEARD
                     SGRLPEIIAFSAAGAEHPLAAEIIAATEGAGGEAIETTPDILSKMSGKDNPQLLIAAY
                     RQPDTSLERIDRSAAPLWIAAQALRDPGNLGTILRTGDAAGAGGLILIDDCADPFSVE
                     AVRASMGALFTQAIATARWDEFLAWLRAGPAQLVGTSLKADRDYLDAEYRAPCFLLIG
                     NEQQGLPAEYEAECDLLVKIPMAGRADSLNAAMAAAVMAFQVRASWRNSGPPGDLIQA
                     "
     gene            complement(2643..2915)
                     /locus_tag="VFU20_09590"
     CDS             complement(2643..2915)
                     /locus_tag="VFU20_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011241128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEU5286738.1"
                     /translation="MTALRRDVRIGNQRGLHARASAKFVSMASQLPATVEVEKDGHRV
                     CGTSIMGLMMLGAAMGDEITIHVEGEGAAEALEQLAELVESRFGED"
     gene            complement(2912..3319)
                     /locus_tag="VFU20_09595"
     CDS             complement(2912..3319)
                     /locus_tag="VFU20_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010163554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5286739.1"
                     /translation="MIGLVLVTHGRLAAEFVTAMEHVVGPQEAIEAICIGPDDDMEAR
                     RKDIAAAIGRVDGGDGAIILTDLFGGTPSNLAISLMKSENIEVIAGVNLPMLIRLESA
                     RKVMGVHAAVTAAREAGRKYISVASEILGEAAA"
     gene            complement(3316..4254)
                     /gene="rapZ"
                     /locus_tag="VFU20_09600"
     CDS             complement(3316..4254)
                     /gene="rapZ"
                     /locus_tag="VFU20_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEU5286740.1"
                     /translation="MNERRPGRRPAPRLLLVTGMSGAGKSTVLDALEDMGWDCVENLP
                     AAMLRDYAEHGLAGGRQVPIAVGMDVRSRGFDPAALPDILHSIERVSPEILYLDCSGA
                     ELIRRYDETRRVHPLAPDRPAEDGIARERSFTADLRSAAESVLDTTDLTPGELRDELR
                     RRYGGEADQPVLTIASFGFARGVSRTADLMFDLRFLANPHWVDELRPLTGTDAKVRAY
                     IEQDPAWTGAMDRIEALLIDWIPRYWAAGKTYLTVAFGCTGGRHRSVCAAAEMAERLR
                     RAGFSPNVRHRDLASRPSDTVESNAGRGSVGGELNG"
     gene            complement(4285..4725)
                     /locus_tag="VFU20_09605"
     CDS             complement(4285..4725)
                     /locus_tag="VFU20_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphatase C-terminal
                     domain-containing protein"
                     /protein_id="HEU5286741.1"
                     /translation="MSARLSSETVHASTVAAEGRAVLISGPSGSGKSDLTLRLLDRGF
                     TLVSDDQTIVRREGDRLIASPPATIADKLEIRGIGIVDMENLTDIPVALLVELTSDIQ
                     RLPDDSRERPILGVPLPLISIDAMAASAPSKVALALDRMGLKLK"
     gene            complement(4722..6287)
                     /locus_tag="VFU20_09610"
     CDS             complement(4722..6287)
                     /locus_tag="VFU20_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007714310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5286742.1"
                     /translation="MSDDLHPDEGDDLALSWSGRWTLAHRILAVNILTLALLALSILY
                     LDAFRNQLSKERIQQTKREAAITAEMLEAVPAKDRARAIAAAGEASGSRIRLYDERGR
                     LAADSWRLTGPTYQLRDPATQSWTKDVARALDRGFNAIVGAEPVEDFSEPGRDSLAAW
                     PEVLEAKSSGRVESEVRNAPDLTPVFSSAAPAAGGVLLVTENDRNFTRTVRSQRAALG
                     IAMALATILSILLSLFLARTIVRPLRRLALAAHRVRLGRAREVKVPRLPSRSDEIGLL
                     ARAVSDMSQSLRLRIDNIEAFAADVTHELKNPLASLRSALDSLDRIDDPRLRQRLIEV
                     AREDVVRLDRLIGDISEAARTDAELARAAFEPVDLGPLIEQLVASWENRREKGDATIA
                     FARPRRASAVVLGKPDRLARAIDNVIDNAISFSPSGGLVEIAATRVGDKVRIRIDDEG
                     PGVPSDAREAIFNRFHSVRPEGENFGRHSGLGLAIARAIVEGHDGEIDVQDRDDAPSG
                     ARFTICLPAANLS"
     gene            complement(6280..6990)
                     /locus_tag="VFU20_09615"
     CDS             complement(6280..6990)
                     /locus_tag="VFU20_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004208080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5286743.1"
                     /translation="MSQVIALVDDDRNILTSISIALQAEGFITRVYSDGETALKAFAD
                     NPPDLGVFDIKMPRMDGMELLRRVRETSSMPVIFLTSKDDELDEALGLAMGADDYISK
                     PFSQRLLIARIRALLRRQELARGEASAAGDEPEAPLLERRRLTMDPARHKVLWDGKDV
                     TLTVTEFLILEALAQRPGVVKSRNQLLDIAYQDDVYVDDRTIDSHIKRIRRKFRAVDP
                     EFDAIETLYGVGYRFEDE"
     gene            7204..>8097
                     /locus_tag="VFU20_09620"
     CDS             7204..>8097
                     /locus_tag="VFU20_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010124365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEU5286744.1"
                     /translation="MSDRIPRHGLEAHGIATGAEVFWNLTTAPLVEHAVHRGEGLLAR
                     DGPLVVRTGKHTGRSAQDRFIVRDATTERTVWWGKFNKPMDGEAFDRLHHDFLEALGA
                     RDSLFVVDLYGGSQPEHRVRVRVVNELAWHNLFIRTLLVRPEAEELPGFEPEYLIIDL
                     PSFKADPQRHGCRSETVIAVNLEKKVILIGGTAYAGEMKKSVFGLLNFLLPAQGIMPM
                     HCSANIGPDGDTAIFFGLSGTGKTTLSADPNRTLIGDDEHGWSDTAVFNFEGGCYAKM
                     IRLSAEAEPEIFATTRRFGTVL"
BASE COUNT         1230 a         2762 c         2849 g         1256 t
ORIGIN      
        1 tgactccggt cgaggcgctg ctcaaggcct atcaggagaa gctccagtcg gtcgagaagg
       61 agcgcgtcga ccactatgcc gggctgcgcg aggcggtcga gctggtccgg acagggcagg
      121 gacaggtgcg tgacgagacc cgcaacctgg tcaacgcgct gcgctcgtcg cccaaggccc
      181 gcgggcgctg gggcgagcag agcctcaaga acgtgctcga gcagtcgggc ctcagcccct
      241 acgccgattt ccgaaccgaa gtgtcggtcg acggcgagga cggcaagctg cgccccgacg
      301 tcgtcgtccg gctgcccggc ggccgccagc tgatcatcga cgccaaatgc tcgctgaacg
      361 cctatctcga cgcctgcgac gaggtcgacg aggacaagcg ccaggcttgc ttccgagccc
      421 acgtcgcctc gatccgcaac cacgcgcagc aactgggctc caaggcctat tgggcgcagt
      481 tcggcgattc ggccgactat gtcgtgatgt acatcccggg cgagcatttc ctgaccgccg
      541 cgctcgagca ggacgacggg ctgtgggact gggcgttcga gcgccgcgtg ctgctcgcga
      601 ctccgaccaa tttggttgcc atcgcgcgga ccgtcgcgag cgtctggagg caggagcggc
      661 taaccgaggc agcggaagaa atcgccaatc tcggcaagga gttgcactcg cgtctttcga
      721 ccatgtcgca gcatatggaa aaggtcggca agaacctcgc gaccgccaac accgcctaca
      781 accagatggt cggcagcttc gagagccagg tgctgaccca ggcgcggcgg ttcgagacgc
      841 tcggcgcggg cagcgccaag gagctcgaca ctccgccgat ggtcgaggcg agcccgcggc
      901 cactgaccaa gatcgccgcg cgggcgtccg acgaggaggt cgcggcggaa tgacagccta
      961 gggcgccggg tcgagcgcga tcgagccggc ggcgttgctg aggtacatgc gctcgttgct
     1021 gctgaaggcg atccgcatcg gctggccgag caccgccgag ccctcgcgct cgagggtccc
     1081 ggccggctcg gggcagccca tcagcgtggt ggcgagattg ccgaccgtca gcaggtcgcc
     1141 ggcgagccgg tattgccctc ccatcgaatt gcacccgaag cgcgcgccta ttccgctact
     1201 cgtatcgaag cgcatcgaat aatcgccggc tgccgacgtc ggccggctgt tgaccgcaac
     1261 cacgcgccag gccgtgccgg cgagatcggt aatcggcgcg ggcgccgtcg gcgtgaccgc
     1321 gcaggcctgc agagccccgg caagcccgag cgccacggcc atggctgaac ggcgcacggc
     1381 ctacagaccg ccgcagccgt ggaaggcgcg gccgtcgacg tcgacccgca cccggtcggg
     1441 atagacccgg tcgctctcgc cgtcgcggca cagagcgtgg acgatgctca cccgaacgcg
     1501 cggcgactgg taggtcgtgc cggcagcggt gatggcgggc accggagtcg gctcgcggaa
     1561 caccggctgg tcggcgcgga tgaaggtcac gtgctgctcg tcgatgatca gcgaccagtc
     1621 gggctgggtg ccgcgggcat ggtaaggggc ggcgggcgcc gggggcgggg tgatgcaccc
     1681 tgtcaccaag gcggcgagca gcgccggcgg cgcgagcgag agcagtcggt tcatgcctgg
     1741 atcaaatccc caggcggccc gctgttccgc cagctggccc ggacctggaa cgccatcacc
     1801 gccgccgcca tcgccgcgtt gaggctgtcg gcgcgcccgg ccatcgggat cttgaccagc
     1861 aggtcgcact cggcctcgta ttcggcgggc agcccctgct gctcgttgcc gatcagcagg
     1921 aagcaggggg cgcggtattc ggcgtcgaga tagtcccggt cggccttgag gctggttccg
     1981 accagctgcg cgggccccgc gcgcagccag gcgaggaact cgtcccagcg cgcggtcgcg
     2041 atggcctgcg tgaacagcgc gcccatcgac gcccgcaccg cttcgaccga gaatgggtcg
     2101 gcgcaatcgt cgatcaggat cagcccgccg gcgccggccg cgtcgccggt gcgcaggatg
     2161 gtgccgaggt tgccggggtc gcgcagcgcc tgggcggcga tccacagcgg cgccgccgag
     2221 cggtcgatcc gctccagcga cgtgtccggc tggcgatagg ccgcgatcag caactgcgga
     2281 ttgtccttgc ccgacatctt ggaaaggatg tcgggcgtcg tttcgatcgc ctcgccgccg
     2341 gcgccttcgg tcgcggcgat gatctcggcg gcgagcggat gctcggcccc cgcggcggaa
     2401 aaagcgatga tttcgggcag gcgcccgctg tcgcgcgctt cggcgacgat ccgcagcccc
     2461 tcggcgagga acagcccttc cgagcggcgc gccttcttgt cgcgcagcga gcgcagccgc
     2521 ttgacggtgg agttggaaaa ggcggtgatc aggcggggca tcaggtcgtc attgcgagcg
     2581 cagcgaagca atccaggctg gattgccgcg tcgctgcgct cctcgcaatg acgtcatcat
     2641 cgtcagtctt cgccgaaccg gctttcgacc agctcggcga gctgctcgag cgcttcggcc
     2701 gcgccttcgc cctcgacgtg gatggtgatc tcgtcgccca tcgccgcgcc aagcatcatc
     2761 agccccatga tcgaggtgcc gcacactcgg tggccgtcct tctcgacctc gacggtcgcc
     2821 ggcagctggc tcgccatgct gacgaacttg gcgcttgccc gggcgtgcag cccccgctgg
     2881 ttgccgatcc ggacgtcgcg ccgcaatgcc gtcaagccgc ggcttccccg aggatttccg
     2941 aggcgaccga gatatatttg cgcccggcct cgcgagcggc ggtcaccgcg gcatgaacgc
     3001 ccatcacctt gcgggcgctt tcgagccgga tcagcatcgg cagattgacc ccggcgatga
     3061 cctcgatgtt ctcgctcttc atcaggctga tggcgaggtt cgacggagtc ccgccgaaca
     3121 ggtcggtgag gatgatcgct ccgtcgccgc cgtcgacccg gccgatcgcc gcggcgatgt
     3181 ccttgcggcg cgcttccatg tcgtcgtcgg ggccgatgca gatcgcctcg atcgcctcct
     3241 gcggcccgac gacatgctcc atcgcggtga cgaactcggc cgcgaggcgg ccatgagtca
     3301 ccagcactag tccaatcatc cgttcaactc gcctccaacg gagcctcgcc cggcattgct
     3361 ttcgaccgtg tcgctcggcc gcgaagccag gtcgcggtgc cggacattcg gggaaaagcc
     3421 cgcccttcgc aatcgttccg ccatttcggc ggcggcacaa acggagcggt ggcgcccccc
     3481 ggtgcagccg aatgcgacgg tcagataggt tttgccggcg gcccagtagc ggggaatcca
     3541 gtcgatgaga agcgcttcga tgcggtccat cgccccggtc caggccgggt cctgctcgat
     3601 ataggctcgg accttggcat cggttccggt cagcgggcga agttcgtcga cccagtgcgg
     3661 gttggcgagg aagcgcaggt cgaacatcaa gtcggcggtg cgcgacacgc cgcgggcgaa
     3721 gccgaacgag gcgatggtca gcaccggctg gtcggcctcg ccgccgtagc gccgccgaag
     3781 ctcgtcgcgc agctcgccgg gcgtcaggtc ggtggtgtcg agcacgctct cggcggcgct
     3841 tcgaagatcg gcggtgaagc tgcgctcgcg ggcgatgccg tcctcggccg gccggtcggg
     3901 cgccagcggg tggacccggc gggtctcgtc gtagcggcgg atcagctcgg ctcccgagca
     3961 atcgaggtag aggatctccg ggctgacgcg ttcgatcgaa tggaggatgt cgggcagggc
     4021 ggcgggatcg aagccgcggc tgcggacgtc catcccgacc gcgatcggca cctggcgccc
     4081 gccggcgaga ccgtgctcgg catagtccct gagcatcgcg gccggcagat tctcgacgca
     4141 gtcccagccc atgtcctcga gcgcgtcgag caccgtcgac ttgccggccc ccgacatccc
     4201 ggtgacgagc agcaggcgcg gcgcaggtcg ccgacccggc cgcctttcgt tcaccgaagg
     4261 gactcgctca acgaagcgtc ccgctcattt gagcttgagg cccattcggt cgagcgcgag
     4321 ggcgaccttg gacggcgccg aggcggccat cgcgtcgatg ctgatcagcg gcagcgggac
     4381 tccgaggatc ggccgctcgc ggctgtcgtc cggcaagcgc tggatgtcgc tggtcagctc
     4441 gaccagcagc gcgaccggaa tgtcggtgag gttctccatg tcgacgatgc cgatgccgcg
     4501 gatctcgagc ttgtcggcga tcgtcgcagg cggcgaggcg atcagccggt cgccctcgcg
     4561 cctcacgatg gtctggtcgt cgctgacgag cgtgaagccg cggtcgagca ggcgcagcgt
     4621 caggtcggac ttgccggaac ccgagggccc gctgatcagg accgcccggc cctcggcggc
     4681 gacggtgctg gcgtgcaccg tctcggacga caggcgcgcg ctcatgacag gttcgccgcc
     4741 ggcaagcaga tggtgaagcg ggcgcccgaa ggcgcgtcgt cgcggtcctg cacgtcaatc
     4801 tccccgtcgt ggccttcgac aattgcgcgg gcaatcgcaa gcccgagccc cgaatggcgg
     4861 ccgaaattct cgccctcggg cctgactgag tggaagcggt taaagatcgc ttcacgggcg
     4921 tcgctcggca ctcccgggcc ctcgtcgtcg atccggatcc ggaccttgtc gccgacccgc
     4981 gtcgcggcga tctcgaccag cccgccggaa ggcgagaagc tgatcgcatt gtcgatcaca
     5041 ttgtcgatcg cccgggcgag ccggtcgggc ttgccgagca ccaccgcgct ggcgcgccgc
     5101 ggccgggcga aggcgatggt ggcgtcgccc ttctcgcggc gattctccca gctcgcgacc
     5161 agttgctcga tcagcgggcc cagatcgacc ggctcgaacg ccgcccgggc gagctcggcg
     5221 tcggtccggg ccgcctcgct gatatcgccg atcagccggt cgagccggac gacgtcctcg
     5281 cgcgcgacct cgatcagccg ctgccgaagt ctggggtcgt cgatccgatc gaggctgtcg
     5341 agcgccgagc gcaacgaggc gagcgggttc ttgagctcgt gggtgacgtc ggcggcgaag
     5401 gcctcgatat tgtcgatccg aagccgcagc gactggctca tgtcggacac cgcgcgggcg
     5461 agcagcccga tctcgtcgct gcgcgagggc agtcggggga ccttgacctc gcgcgcccgg
     5521 ccgaggcgca cgcggtgggc ggccagcgcc aggcgcctga gcggccggac gatggtccgg
     5581 gcgaggaaca gcgacagcag gatcgacagg atcgtcgcca gcgccatggc gatgccgagc
     5641 gcggcgcgct ggctccggac cgtgcgggtg aagttgcggt cgttctcggt gaccagcagc
     5701 accccgcccg cggcgggcgc ggcggacgag aagaccggcg tcaggtcggg tgcgttgcgg
     5761 acctcgctct cgacccggcc gctgctcttc gcctccagca cctccggcca ggcggcgagc
     5821 gagtcgcgac cgggctcgga gaaatcctcg accggctcgg cgccgacgat cgcgttgaag
     5881 ccgcggtcga gcgcccgggc gacgtccttg gtccagctct gggtcgccgg gtcgcgcaac
     5941 tggtaggtcg ggccggtcaa ccgccagctg tccgccgcca gccgcccgcg ctcgtcgtac
     6001 agccggatcc ggctgccgct cgcctcgccc gccgcggcga ttgctcttgc ccggtccttc
     6061 gccggcacgg cctcgagcat ctcggcggtg atcgcggctt cgcgcttggt ctgctggatg
     6121 cgctccttgc tcagctggtt gcggaacgcg tcgaggtaga gaatgctgag cgccagcagc
     6181 gccagggtca ggatgttcac cgcgaggatc cggtgggcga gggtccagcg ccccgaccag
     6241 ctcagcgcaa ggtcgtcgcc ctcgtccgga tggaggtcgt cactcatctt cgaagcggta
     6301 gccgacgccg tagagggtct cgatcgcatc gaactcgggg tcgaccgccc ggaacttgcg
     6361 ccggattcgc ttgatgtggc tgtcgatcgt ccggtcgtcg acatagacgt cgtcctggta
     6421 ggcgatgtcg agcagctggt tgcggctctt caccaccccc ggccgctggg cgagcgcctc
     6481 gaggatcagg aactcggtca cggtcagggt gacgtccttg ccgtcccaca gcaccttgtg
     6541 ccgggccggg tccatggtca gccggcggcg ctcgagcagc ggcgcctcgg gctcgtcgcc
     6601 ggccgcgctg gcctcgcccc tggcgagctc ctgccggcgc agcagggcgc ggattcgggc
     6661 gatcagcagc cgctgcgaga agggcttcga aatatagtcg tcggcgccca tcgccagccc
     6721 gagcgcctcg tcgagctcgt cgtccttgga ggtcaggaag atgaccggca tcgagctggt
     6781 ctcgcgcacc cggcggagca gctccatccc gtccatgcgc ggcatcttga tgtcgaacac
     6841 gccaaggtcg ggcgggttgt cggcgaacgc cttcagcgcc gtctccccgt ccgaatagac
     6901 gcgggtgatg aagccttcgg cctgaagcgc gatggatatc gacgtgagga tgttgcggtc
     6961 gtcgtcgacc agcgcgatca cttggctcat caatggcctt ctggcggcgg cttgcgccgg
     7021 gagcctcggc ttagccagcg tctccagccg aaacaagcgc cgcccctccc caggggccgc
     7081 ggctttgacc cggcgagttt ttcggggtta taggcgcgag tgcgcatacg tatgcggcgg
     7141 caggccccct tgtcccaagg tcccggccgc cgccgctttg accatcggca ggggaaaaat
     7201 cgagtgagcg accggattcc aaggcacggg cttgaagcgc acggcatcgc gaccggcgcc
     7261 gaggtcttct ggaatctgac caccgcgccg ctggtcgagc atgccgttca tcgcggcgaa
     7321 ggcctgctcg ccagggacgg gcccctggtc gtccgcaccg gcaagcatac cggtcgaagc
     7381 gcccaggacc gcttcatcgt ccgcgacgcg actaccgagc gcacggtctg gtggggcaag
     7441 ttcaacaagc cgatggacgg cgaggcgttc gaccggctgc accacgactt cctcgaagcg
     7501 ctcggcgcca gggacagcct gttcgtcgtc gacctctacg gcggctcgca gcccgagcac
     7561 cgggtgcggg tccgggtggt caacgagctc gcctggcaca atctgttcat ccgcaccctg
     7621 ctggtccggc ccgaagcgga ggagctgccg gggttcgagc ccgaatatct gatcatcgac
     7681 ctgccgagct tcaaagcgga cccgcagcgc catggctgcc ggagcgagac ggtgatcgcg
     7741 gtcaacctgg agaagaaggt catcctgatc ggcggcaccg cttatgccgg cgagatgaag
     7801 aagtcggtgt tcggcctgct gaacttcctg ctcccggcgc aggggatcat gccgatgcac
     7861 tgctcggcca acatcggccc cgacggcgac acggcgatct tcttcggcct gtccgggacc
     7921 ggcaagacga ccctgtcggc cgaccccaac cggaccctga tcggcgacga cgagcacggt
     7981 tggtcggaca ccgcggtgtt caacttcgag ggcggttgct acgccaagat gatccgcctg
     8041 tcggccgagg ccgagccgga gattttcgcg acgaccaggc gcttcgggac ggtgctc
//
LOCUS       DASTZO010000341         9641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_337855,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000341 DASTZO010000000
VERSION     DASTZO010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9641
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337855"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1726
                     /locus_tag="VFU20_09625"
     CDS             <1..1726
                     /locus_tag="VFU20_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286745.1"
                     /translation="AAEAARTDIAVLLEDLPRAEATSRAIAEQLRGIGSDSASRTAEL
                     SEQVAALSERTREADEQVGSAAQRLDTHLTQIESAGAAAAARVGEAETSFSGALDSLL
                     DRTARTLDEIRGGIDTQAAAVAALVEQASAGIGRAGIEAADVLGSKIGQAGASLDGLS
                     ARVAEQERVSQRLFADIDRGLTLIDERFAELAAQGDERANHFLGSLSRARAELDGLAQ
                     QTGAQDHSIEALATRTAALRQGIDQLTAEIQEGLGTAIGEAETGAERVLKATEAAKPE
                     IGWMRDAAVEASQQIAASGAGIADQQDRFAALLATIDDGVGDSEQRLAALTAALSEAQ
                     GEASRLTAETGPALVAALVQVKEAAAHAAERAREAIEGVIPDSAGKLSKATREALEAA
                     IREGIEERLRGVEAIAARAVESAREASDRLTQQMLSLGQSSAALEHYFEQTQADQREK
                     DSEAFARRVSLLIDSMNSAAIDVGKILSDEVDDKAWASYLKGNRGVFTRRAVRLMGNS
                     ESRAIQAHYDTDREFQGSVNRYVHDFEAMLRRVLAERDGGMMAVTLMSSDMGKLYAAL
                     AQAIDRRR"
     gene            complement(1742..2008)
                     /locus_tag="VFU20_09630"
     CDS             complement(1742..2008)
                     /locus_tag="VFU20_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HEU5286746.1"
                     /translation="MTVGSIVAIYLLFFIASAFLLLPFGVKTDEEAGTPKVPGQADSA
                     PHRFDLPRHLLRAALLGLLLFALYYANWTQGWIEVDDLDFYNPR"
     gene            complement(2005..3645)
                     /locus_tag="VFU20_09635"
     CDS             complement(2005..3645)
                     /locus_tag="VFU20_09635"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEU5286747.1"
                     /translation="MIPGEELLFCALGGSGEIGMNVNLYGCRGQWLMVDLGLTFAGPD
                     YPGIDLILPDLEFIERQQDRLAGIILTHGHEDHIGALPYLCSDLKVPLYATPFTAGLI
                     AGKLEEEGLTGQVRLNIVEGGGPIELGPFKVRFIALSHSIPEGNGVLVETPFGRVFHT
                     GDWKIDETPVLGEAPGSASLIEIGELGVLALVCDSTNVFQNEPSGSEEGVHHGLAEQV
                     AKARGRVLVTTFASNAARLQTIGRVASETGRRVCVAGRSLDRIISVAKATGYLRDFPE
                     PVRFDEAMRLPREQVLIVATGGQGEPRAALGRIASGNHELKLAEGDTIIFSSRIIPGN
                     EVAIGRIMNQLSDIGALIVTEKQAHVHVSGHPGRPELLMMYDWVRPEIIVPVHGEARH
                     LAEQARLALSHGVPKAVVQMNGDLVRLAPDGPAKIDEVRVGRLVLDGDVILPADGTTI
                     NERRKIAVGGLITVALAVTAGGKLAGPPLVRPSGVPVEADRDDFIADATDTAERAFDP
                     RADEERAREAVRLAVRRCATAWTGKKPVVDVSIVKVGE"
     gene            complement(3646..4425)
                     /locus_tag="VFU20_09640"
     CDS             complement(3646..4425)
                     /locus_tag="VFU20_09640"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEU5286748.1"
                     /translation="MLLAIDAGNTNLVFALLQDGEIKARWRIATDPRRTADQYAVWLH
                     QLLELEGYARADVDSVIIGIVVPRALHNLEVLARKYFHVEPLIAGQGAAAWPMALDVD
                     EPHNVGADRALNVIAAHAKYPGDLVIIDFGTATTFDAVDGSGAYKGGIIAPGINLSID
                     ALVNAAAMLPRIAIDAPDDTSVVGRTTERQMLIGIYWGYVAMIEGLVERMKKEIAGPV
                     TVIATGGLAALFDKHTQAFDAIEPDLTIQGLGLLHQTSVSA"
     gene            complement(4455..5156)
                     /locus_tag="VFU20_09645"
     CDS             complement(4455..5156)
                     /locus_tag="VFU20_09645"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU5286749.1"
                     /translation="MTRIRIVERTGSTNADLLADANAAESEWLVALQQSAGKGRQGRA
                     WAGGEGNFFGSTLIGLKPGDPPAPLLSLAAGLALVEAVDVAAPGLSSTQGLLMLKWPN
                     DLLLGGDKLGGVLLERAGERVVAGFGVNLGSAPSFPDRKAASLQAAIAPKAFAPLLAA
                     SFARALALWRSGASNALADAWLERAHPVGALLTVHTGADDRTSGRFDGLEPDGALRLR
                     TDQGVEIVRAGDVEL"
     gene            complement(5153..6589)
                     /gene="nuoN"
                     /locus_tag="VFU20_09650"
     CDS             complement(5153..6589)
                     /gene="nuoN"
                     /locus_tag="VFU20_09650"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010402613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HEU5286750.1"
                     /translation="MNQLMPILPELILTAGAIVLMMVAAFAGRRGSGLTSWASVALLV
                     AATVALIGPPSSAGPLFGGLIVADAFGSFGKAIIFLSSAVAIIAAHGWFERDTEHSSE
                     YSVLILFSAVGGAVMVSATNLITLYMGLELTSLSSYVLASYRRGDERSAEAGLKYFVL
                     GALASGILLYGISLLYGFTGTMTFPGLAAAFARDGALSLGLLFGLVFLLAGIAFKVSA
                     VPFHMWTPDVYEGAPTPVTAFFASAPKVAAVLLATRVCLDALEPATDAWRQIVIFAAL
                     ASILLGAVAAWGQTNIKRLLAYSSINNVGFALIGLAAGGPKGASSVLFYMAVYVVMTL
                     GAFLCVLRMRTEGGEPTERIDDLAGLSQARPAFAAALAVFMFSLAGVPPLFGFWPKLL
                     VFNAAVDEGLIALAVAGIVGTVVGAYYYLRIVKIMYFDEAASPLVRTRAPLERLMILV
                     AALIVSPLGYLLIGPLGAYADRAAGSIF"
     gene            complement(6589..8139)
                     /locus_tag="VFU20_09655"
     CDS             complement(6589..8139)
                     /locus_tag="VFU20_09655"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU5286751.1"
                     /translation="MSGLDDLPVLSILVGLPLVAGMACLFVNAAAARWIALVTTLAAL
                     AIGAWLWGSYDPAGAQWQFVERLDLGGGIAWALGIDGIALVLIMLTLFLMPICIGASW
                     RSIEKRVPEYMAAFLLMQALMVGVFVAQDLFLFYIFFEGGLIPMYLIIGIWGGPERIK
                     ASYKFFLYTLLGSVLMLIAMLYMVLEAGTTSIPELMAFDFDPDAQRWLWLAFLASFAV
                     KLPMWPVHTWLPDAHVEAPTAGSVILAGVLLKMGGYGFIRFSLPMFPEGSAAFVPLVF
                     VLSGIAVVYTSLVALVQRDMKKLIAYSSVAHMGFVTFGLFAFNRQGIEGAMIVMLSHG
                     LVSGALFLCVGVVYDRLHTRDIDRYGGLSNNMPGYALLFMLFTMAAVGLPGTSGFVGE
                     FLSLVGTYRVSTWAAIVATTGIILGAAYMLWLYWRIAFGAARTAEAAAMPDLSMREWW
                     LLGPIAAAVLWMGVYPESFLRPIRADVGRLLERVERAAPKGDSLPTAGKPQAGTHAPE
                     TREPEAAH"
     gene            complement(8136..>9641)
                     /gene="nuoL"
                     /locus_tag="VFU20_09660"
     CDS             complement(8136..>9641)
                     /gene="nuoL"
                     /locus_tag="VFU20_09660"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003051616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU5286752.1"
                     /translation="GFSLGIFGTFLVFGTVSIPAILAAAPSMAGSTIGFAGMTVDTMT
                     LLCLLLFVGACGKSAQLGLHTWLPDAMEGPTPVSALIHAATMVTAGVFMVCRLSPMFE
                     ASEIARHAVIYVGAATAIFAATVGTAQNDIKRVVAYSTCSQLGFMFFAAGVGAYGAAM
                     FHLFTHAFFKALLFLGAGSVIHAMHHEQDMRFYGGLRRQIPFTFWVMVIGTLAITGVG
                     IFGLGFAGYWSKDSILESAYASGSVPGGIAFFLGALAALLTSFYSWRLIFLTFYGEPR
                     WAASEHIQHALHGDHDSPDQEHGDSSHDTGMPPVGSGGYRPHESPWTMLVPIGLLALG
                     SILAGQLFHEIFVGAEEGPRFWGGSVAFDAHLAHAAHEVPAWVKYTATFVMLIGLAIA
                     WNNYIRRPDAAGRFVAQFRGLHRFVLNKWYFDELYDRIFVRPALWLGRLFWHGGDEGT
                     IDRFGPHGAAYAVGVGNRWTARLQSGYLYSYALVMLLGLIGAATWAVWWAR"
BASE COUNT         1707 a         3246 c         3404 g         1284 t
ORIGIN      
        1 cgcggccgaa gcggcgcgca ccgacatcgc cgtcctgctc gaggatttgc ccagggcgga
       61 ggcgacgagc cgggcgatcg ccgagcagtt gcgcggcatc ggcagcgatt cggcgtcgcg
      121 caccgccgaa ttgagcgaac aggtcgcggc gctgtccgag cgcacccgcg aggccgatga
      181 gcaggtcggc agcgcggccc agcggctgga cacgcacctg acccagatcg aaagcgccgg
      241 ggccgcggcc gcggcccggg tcggcgaggc ggaaacgagc ttttcgggtg cgctcgactc
      301 gttgctcgac cggacggccc ggaccctcga cgagatccgc ggcgggatcg acacccaggc
      361 cgcggcggtg gcggcgctgg tcgagcaggc gtcggccggg atcggccgcg ccgggatcga
      421 ggcggccgac gtgctcggct cgaagatcgg ccaggccgga gcgtcgctcg acgggctgtc
      481 ggcgcgggtc gccgagcagg agcgcgtgtc gcagcgcctg ttcgccgaca tcgaccgcgg
      541 gctcactctg atcgacgagc gcttcgccga attggcggcg cagggcgacg agcgcgccaa
      601 ccatttcctc ggctcgctgt cgcgcgcccg cgccgagctc gacgggctcg cgcagcagac
      661 cggcgcgcag gatcattcga tcgaggcgct ggcgacgcgc accgccgccc tgcgccaagg
      721 catcgaccag ctgaccgcgg agatccaaga aggcctcgga accgcgatcg gcgaggccga
      781 gaccggcgcc gagcgggtgc tcaaggcgac cgaggccgcc aagccggaga tcggctggat
      841 gcgcgacgcg gcggtcgagg cgagccagca gatcgccgcc agcggcgccg ggatcgccga
      901 ccagcaggac cgcttcgccg cgcttctggc aaccatcgac gacggcgtcg gcgattccga
      961 gcagcggctg gcggctttga ccgcggcgct gagcgaggcg cagggcgagg cctcgcgact
     1021 gaccgccgag accgggccgg cgctggtcgc agcgctggtc caggtcaagg aggccgccgc
     1081 ccatgccgcg gaacgcgccc gagaggcgat cgagggagtc attcccgaca gcgccggcaa
     1141 gctgtcgaag gcgacccgcg aggcgctcga ggcggcgatc cgcgagggca tcgaggagcg
     1201 gctgcgcggc gtcgaggcga tcgccgcgcg agcggtcgaa agcgcccgcg aggcgtcgga
     1261 ccggctgacc cagcagatgc tcagcctcgg ccagagcagc gccgcgcttg agcattattt
     1321 cgagcagacc caggccgacc agcgcgaaaa ggacagcgag gcgttcgccc gccgggtgtc
     1381 gctattgatc gattcgatga attccgcggc gatcgacgtc ggcaagatcc tgtccgacga
     1441 ggtcgacgac aaggcctggg catcctacct caagggcaac cgcggcgtgt tcacgcgccg
     1501 cgccgtgcgg ctgatgggga acagcgagag ccgcgcgatc caggcgcatt acgacaccga
     1561 ccgcgagttc caggggtcgg tcaaccgcta cgtccacgat ttcgaggcga tgctgcgccg
     1621 ggtgctggcc gagcgcgacg gcgggatgat ggcggtgacc ctgatgtcct cggacatggg
     1681 caagctctac gcggcgctcg ctcaggcgat cgatcggcgg cgctgagctt cagcgcggct
     1741 ttcagcgcgg attgtagaag tcgaggtcgt cgacctcgat ccagccctgc gtccaattgg
     1801 cgtaatagag cgcgaacaga agcagcccga gcagcgcggc gcggagcagg tgccgcggca
     1861 ggtcgaagcg atgcggggcg ctgtcggcct gccccgggac cttgggagtc cccgcctcct
     1921 cgtcggtctt caccccgaac ggcaacagga ggaacgcgct ggcgatgaag aacagaagat
     1981 agattgcgac gatcgagccg accgtcattc gccgaccttg acgatcgaca cgtcgaccac
     2041 cggcttcttg ccggtccagg cggtcgcgca gcggcggacc gcgagccgca ccgcctcgcg
     2101 cgcccgctcc tcgtcggcgc gcggatcgaa ggcgcgctcg gcggtgtcgg tcgcatcggc
     2161 gatgaaatcg tcgcggtcgg cttcgaccgg gactccagac ggccgcacca gcggcggccc
     2221 ggcgagcttg ccgccggcgg tcacggccag cgcgacggtg atcagcccgc cgaccgcgat
     2281 cttgcggcgc tcgttgatcg tcgtcccgtc ggccggaaga atgacgtcgc cgtcgagcac
     2341 cagccggccg acgcgcacct catcgatctt cgccggccca tccggggcga ggcgcacgag
     2401 gtcgccgttc atctggacca ccgccttggg gactccatgc gacagcgcca ggcgcgcctg
     2461 ctcggcaagg tgccgcgcct cgccgtgcac gggcacgatg atctccggcc ggacccagtc
     2521 gtacatcatc agcagctcgg gccgacccgg gtgtccggag acatggacgt gggcctgctt
     2581 ctcggtgacg atcagcgcgc cgatgtcgga cagctggttc atgatccggc cgatcgcgac
     2641 ctcgttgccg gggatgatcc gcgacgagaa aatgatcgtg tcgccctcgg cgagcttcaa
     2701 ttcgtggttg cccgaggcga tccgcccgag cgcggcgcgc ggctcgccct ggccgccggt
     2761 ggcgacgatc agcacttgct cacgcggcag ccgcatcgcc tcgtcgaagc gcaccggctc
     2821 ggggaaatcc ctgagatagc cggtcgcctt ggcgaccgag atgatgcgat cgagcgagcg
     2881 cccggcgacg cacacccgcc gcccggtttc gctcgccacc cggccgatgg tctggagccg
     2941 cgcggcgttc gacgcaaaag tggtgaccag aacccgcccg cgagccttgg cgacctgctc
     3001 ggcaaggccg tggtggacgc cctcctccga gcccgacggc tcgttctgga agacgttggt
     3061 cgaatcgcag accagggcca ggactcccag ctcgccgatc tcgatcagcg aggcggagcc
     3121 cggcgcctcg ccgagcaccg gcgtctcgtc gatcttccag tcgccggtgt ggaacacgcg
     3181 cccgaacggc gtttcgacga gcactccgtt gccctcgggg atcgagtgcg acagagcgat
     3241 gaagcggacc ttgaacgggc cgagctcgat ggggccgccg ccctcgacga tgttgagccg
     3301 gacctgtccg gtcagcccct cctcctcgag cttgccggcg atcagcccgg cggtgaacgg
     3361 ggtcgcgtac agcggcacct tcaagtccga gcacagatag gggagcgccc cgatatggtc
     3421 ctcgtggccg tgggtgagga tgatcccggc gagccggtcc tgctggcgct cgatgaactc
     3481 gaggtcggga aggatgaggt cgatgccggg ataatcgggg ccggcgaagg tcaggcccaa
     3541 gtcgaccatc agccactggc cgcggcagcc gtagagatta acgttcatgc cgatctcgcc
     3601 cgacccgccg agcgcgcaga acaacagctc ttcgcctgga atcatctagg ctgaaacgct
     3661 tgtctggtgg agaagcccca acccctggat ggtcaggtcg ggctcgatcg cgtcaaacgc
     3721 ctgcgtatgc ttgtcgaaca gggccgcaag cccgccggtg gcgatcacgg tgaccggccc
     3781 cgcgatttcc tttttcatcc gttcgaccag cccctcgatc atcgccacat agccccagta
     3841 gatgccgatc agcatctggc gctcggtggt ccggccgacg acgctggtat cgtcgggcgc
     3901 gtcgatcgca atccgcggca gcatcgccgc ggcgttgacc agggcgtcga tcgacaggtt
     3961 gatccccgga gcgatgatcc cgcccttgta ggcgcccgag ccatccaccg cgtcgaaggt
     4021 ggtcgcggtt ccgaaatcga tgatgaccag gtcgcccgga tactttgcgt gggcggcgat
     4081 gacgttgagc gctcggtcgg cgccgacgtt gtgcggctcg tcgacgtcga gcgccatcgg
     4141 ccaggccgcc gcgccctgcc cggcgatcag cggctcgacg tggaaatatt tgcgggccag
     4201 cacctccagg ttgtggagcg cgcgcggcac gacgatgccg atgatcacag agtcgacgtc
     4261 ggcccgggca tagccttcga gctcgagcaa ttggtgcagc cacaccgcat attggtcggc
     4321 ggtccgccgc gggtcggtcg cgatccgcca tcgcgccttg atctcgccgt cctggagcag
     4381 cgcaaacacc aggttggtgt tgccggcgtc gatggcgagc agcatggccg tgccctagct
     4441 ttccgcgccc tggttcacaa ctcgacgtcg cccgcgcgga cgatctcgac gccctggtcg
     4501 gtgcgcaggc ggagcgcccc gtcgggctcg aggccgtcga agcggccgct ggtgcggtcg
     4561 tcggcgccgg tgtggacggt cagcaatgcg ccgaccgggt gggcgcgctc cagccacgca
     4621 tcggcgagcg cattcgaagc gccgctccgc catagcgcga gggcccgggc aaagctcgcg
     4681 gccagcaagg gcgcgaaggc cttgggcgcg atcgccgcct ggagcgaggc cgccttgcgg
     4741 tctgggaagc tcggcgccga gccgaggttg accccgaagc cggcgacgac gcgctcgccg
     4801 gcgcgctcga gcaggactcc gccaagcttg tcgccgccga gcagaagatc gttgggccat
     4861 ttgagcatca gcaggccttg cgtcgaggac aagcccggag cggcgacgtc gaccgcctcc
     4921 accagcgcca gccccgcggc cagcgacagc agcggcgccg gcgggtcccc cggcttcagc
     4981 ccgatcagcg tgctgccgaa gaaattgccc tcgcccccgg cccaggcgcg cccctgccgg
     5041 cccttgcccg ccgattgctg cagagcgacc agccactcgc tttcggcggc attggcgtcg
     5101 gcgagcagat cggcattggt cgagccggtg cgctcgacga tccggatgcg ggtcagaaga
     5161 tcgaccccgc ggcgcggtcg gcgtaggcgc cgagcgggcc gatcagcagg taaccgagcg
     5221 gcgagacgat cagcgccgcg accaggatca tcagccgctc gagcggcgcg cgagtgcgca
     5281 ccagcggcga ggccgcctcg tcgaaataca tgatcttgac gatccggagg taataataag
     5341 cgccgaccac ggtgccgacg atgccggcga cggcgagcgc gatcagcccc tcgtcgaccg
     5401 cggcgttgaa caccagcagc ttgggccaga agccgaacag cggcggcacg ccggcgaggc
     5461 tgaacatgaa aaccgccagc gccgcagcga acgccgggcg cgcctgcgac aggccggcga
     5521 ggtcgtcgat tcgctcggtc ggctcgccgc cctcggtccg catcctgagc acgcagagga
     5581 aggcgcctag ggtcatcacc acgtagaccg ccatgtagaa caggaccgac gaggcgccct
     5641 tgggcccgcc ggcggcgagg ccgatcagag cgaagccgac attgttgatc gacgaataag
     5701 cgagcagccg cttgatgttg gtctggcccc aggcggcgac ggcgccgagc aggatggagg
     5761 cgagcgcggc gaagatgacg atctgccgcc aggcgtcggt cgccggctcg agcgcgtcga
     5821 ggcacacccg ggtcgcgagc agaaccgccg cgaccttcgg agccgaggcg aagaaagcgg
     5881 tgaccggtgt cggggcgcct tcgtagacgt cgggcgtcca catgtggaac ggcaccgcgc
     5941 tgaccttgaa ggcgattccg gcgagcagga agacgagacc gaacagcagt ccgagcgaca
     6001 gggcgccgtc gcgcgcgaag gcggcggcga gaccggggaa ggtcatggtg ccggtgaagc
     6061 cgtagagcaa ggagatgccg tagagcagga tgccgctggc aagcgctcca aggacgaaat
     6121 atttgagtcc cgcctcggcg ctgcgctcgt cgccgcggcg gtaggaggcg agcacgtagc
     6181 tcgacaggct ggtgagctcg agccccatgt agagggtgat cagattggtc gccgagacca
     6241 tcaccgcccc gccgaccgcg ctgaacagga tcagcaccga atattcggag gaatgctcgg
     6301 tgtcgcgctc gaaccagccg tgcgcggcga tgatcgcgac ggccgaggag aggaagatga
     6361 tcgccttgcc gaacgatccg aacgcgtcgg cgacgatcag cccgccgaac agcgggccgg
     6421 cgcttgatgg cgggccgatc agagcgacgg tcgcggcgac cagcagagcg accgacgccc
     6481 agctggtcag ccccgagccg cgacggccgg cgaacgcggc gaccatcatc agcacgatcg
     6541 cgcccgcggt caggatgagc tccggaagaa tgggcatcag ctggttcatc agtgcgccgc
     6601 ctccggttca cgggtttcgg gggcgtgggt cccggcctgc ggcttgcccg cggtcggcag
     6661 cgaatcgccc ttgggagccg cgcgctcgac ccgctcgagc agccgcccga catcggcccg
     6721 gatcggccga aggaagcttt cgggatagac tcccatccac agcaccgccg cggcgatcgg
     6781 ccccagcagc caccattcgc gcatcgacaa gtcgggcatc gccgccgctt cggcggtgcg
     6841 cgccgcgccg aaggcgatcc gccagtagag ccacagcatg taggcggcgc cgaggatgat
     6901 gccggtggtg gcgacgatcg ccgcccaggt cgacacccgg taggtgccga ccaggctcag
     6961 aaactcgccg acgaagccgc tggtgccggg cagcccgacc gcggccatgg tgaacagcat
     7021 gaacagcagc gcatagcccg gcatgttgtt cgacagcccg ccgtagcggt cgatgtcgcg
     7081 cgtgtgcagc cggtcgtaga ccaccccgac gcacaggaac aaagcgccgg agaccagacc
     7141 gtggctgagc atgacgatca tcgcgccctc gatgccctgg cggttgaacg cgaacaggcc
     7201 gaaggtgacg aagcccatgt gcgcgaccga cgagtaagcg atcaatttct tcatgtcgcg
     7261 ctgcaccagg gcgaccagcg aggtgtagac gaccgcgatc ccgctgagga cgaacaccag
     7321 cggcacgaac gcggccgagc cttcggggaa catcggcagc gagaagcgga tgaagccgta
     7381 gccgcccatt ttcagcagca cgccggcgag gatcaccgag ccggcggtcg gcgcctcgac
     7441 gtgggcgtcg ggaagccagg tgtggaccgg ccacatcggc agcttgaccg cgaagctggc
     7501 aaggaaagcg agccacagcc agcgctgggc atccggatcg aaatcgaacg ccatcagctc
     7561 ggggatcgag gtcgtgccgg cctcgagcac catgtagagc atggcgatca gcatcagcac
     7621 cgagccgagc aaggtgtaga ggaagaattt gtagctcgcc ttgatgcgct cgggcccgcc
     7681 ccagatgccg atgatcagat acatcgggat caggccgcct tcgaagaaga tgtagaacag
     7741 gaacaggtcc tgggcgacga acacgccgac catcagcgcc tgcatcagca ggaaggcggc
     7801 catatattcg ggcacgcgtt tttcgatgct tctccacgac gccccgatgc agatcggcat
     7861 caggaacagg gtcagcatga tcagcaccaa agcgatgccg tcgatgccga gcgcccaggc
     7921 gattccgccg ccgaggtcga gccgctcgac gaactgccac tgcgccccgg cggggtcgta
     7981 ggagccccac agccaggcgc cgatcgccag cgcggccagg gtcgtcacca gcgcgatcca
     8041 gcgcgcggcc gcggcattga cgaacaggca ggccatgccc gcgaccagcg gcaggccgac
     8101 caggatcgac agcactggca ggtcgtcgag gccgctcacc gcgcccacca gacggcccag
     8161 gtcgcagccc cgatcaggcc gaggagcatc accagcgcat agctgtagag gtagcccgac
     8221 tgcagccggg cggtccaccg gttgccgacc ccgaccgcat aagcggcgcc gtgcggtccg
     8281 aagcggtcga tcgtgccctc atcgccgccg tgccagaaca gcctgcccag ccacagcgcc
     8341 ggccgaacga agatgcggtc gtagagctcg tcgaaatacc atttgttgag gacgaagcgg
     8401 tgcagcccgc ggaactgggc gacgaagcgg ccggcggcgt ccggtcggcg gatgtaattg
     8461 ttccaggcga tcgccaggcc gatcagcatg acgaaggtgg cggtgtattt gacccacgcc
     8521 ggcacctcgt gcgcggcgtg ggcgaggtgc gcgtcgaagg cgacgctgcc gccccagaac
     8581 cgcggcccct cttcggcgcc gacgaagatt tcgtggaaca gctggccggc aaggatcgag
     8641 cccagcgcca gcaggccgat cggcaccagc atcgtccagg ggctttcgtg cggccggtag
     8701 ccgccgctcc cgaccggcgg catgccggtg tcgtgcgagc tgtcgccatg ctcctggtcg
     8761 ggcgagtcat gatctccgtg gagcgcgtgc tggatgtgct cggaggcggc ccagcgcggc
     8821 tcgccgtaga aggtgaggaa gatcagccgc cacgagtaga agctggtcag aagcgcggcg
     8881 agcgcgccca ggaagaaggc gatcccgccc ggaacgctgc cgctcgcata agcgctctcg
     8941 aggatcgaat ccttcgacca atagccggca aagcccaacc cgaagatgcc gacgccggtg
     9001 atcgccagcg tgccgatcac catcacccag aaggtgaacg ggatctgcct gcgaaggccg
     9061 ccgtagaagc gcatgtcctg ctcgtggtgc atcgcgtgga tcaccgagcc cgcgccgagg
     9121 aacagcaagg ccttgaagaa ggcgtgggtg aacagatgga acatcgccgc gccgtaggcg
     9181 ccgaccccgg cggcgaagaa catgaagccc agttgcgagc aggtcgagta agcgaccacc
     9241 cgcttgatgt cgttctgggc ggtgccgacg gtggcggcga agatcgcggt cgccgcgccg
     9301 acgtagatga cggcgtgccg ggcgatctcg ctcgcctcga acatcggcga cagccggcag
     9361 accatgaaca cgcccgcggt gaccatggtc gcggcgtgga tcagggcgct gaccggggtc
     9421 gggccctcca tcgcgtcggg cagccaggtg tgaaggccga gctgcgccga cttgccacaa
     9481 gccccgacga acaggagcag gcacagcaag gtcatcgtgt cgaccgtcat gccggcaaag
     9541 ccgatcgtcg aacccgccat cgacggcgcg gcggcgagga tcgccgggat agacaccgtt
     9601 ccgaagacta agaacgtccc gaagatgcca aggctgaagc c
//
LOCUS       DASTZO010000342         5907 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Sphingomicrobium sp. isolate SMAG_U2470 k141_78134,
            whole genome shotgun sequence.
ACCESSION   DASTZO010000342 DASTZO010000000
VERSION     DASTZO010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960928
            Sequence Read Archive: SRR25158462
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Sphingomicrobium sp. (soil metagenome)
  ORGANISM  Sphingomicrobium sp.
            Bacteria; Pseudomonadati; Pseudomonadota; Alphaproteobacteria;
            Sphingomonadales; Sphingomicrobiaceae; Sphingomicrobium.
REFERENCE   1  (bases 1 to 5907)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5907)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 03:23:30
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,938
            CDSs (total)                      :: 1,897
            Genes (coding)                    :: 1,884
            CDSs (with protein)               :: 1,884
            Genes (RNA)                       :: 41
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 37
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 13
            CDSs (without protein)            :: 13
            Pseudo Genes (ambiguous residues) :: 0 of 13
            Pseudo Genes (frameshifted)       :: 2 of 13
            Pseudo Genes (incomplete)         :: 11 of 13
            Pseudo Genes (internal stop)      :: 1 of 13
            Pseudo Genes (multiple problems)  :: 1 of 13
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5907
                     /organism="Sphingomicrobium sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78134"
                     /isolate="SMAG_U2470"
                     /isolation_source="soil"
                     /db_xref="taxon:1940456"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="33.00277778 N 112.5905556 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            105..1784
                     /locus_tag="VFU20_09665"
     CDS             105..1784
                     /locus_tag="VFU20_09665"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HEU5286753.1"
                     /translation="MREGTAQKISNALLSGAAGFASFVFSLSAMLYITELNEKIVASI
                     VAGAFCLLISYIASERPNSESARALAALGDRLLAVEDGDLVSPAPVAVQRIMPKLAAA
                     VDTLFAEVRSSIENAHALGMYDPVTSLPNRLHFRSEADKLLSEADGSRSAMLFVDLDR
                     FKSVNDSLGHARGDQLLIMVANRLRVVVTAEFGEALQRRPLLARLAGDEFTLFFPAIQ
                     SSADVERIARRIALAIAEPFELCGHSVDIGASIGVSICPDHGSSIESLMRAADIAMYC
                     AKSTGGGQHCLFNEQLAAQHQEKVETERALTEAVQRDEFLLAFQPQLSLVTGEIAGAE
                     ALLRWNHPRDGLRMPDSFVPIAERTGMITDIGDWVVAEVASVLGSWNREGIARRLAFN
                     VSPRQIERADFFSRLRHSFADEGVPLSLIELEFTESAAMEVSEGVLAEIAALRADGAT
                     ISIDDFGTGYSNIARLRTMPLDRVKLDPSLIADIEHCERARVVVQAVIQLIHGVGCEI
                     VAEAVETVAQADILRTMGCQALQGYVFAHPMFEDEFVGWIVNADRGSRSVA"
     gene            complement(1750..3288)
                     /locus_tag="VFU20_09670"
     CDS             complement(1750..3288)
                     /locus_tag="VFU20_09670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5286754.1"
                     /translation="MQGARPDPGSPIVTSLALLAVAFWVHYLLVHDLLGLLTVDEIYF
                     AHVFWLMREGLEPYSGFYSTHLPAYFRILGVLVPPAAPLDLSFVWTLRAVSALVVTAY
                     AILLLKLARRDFLFLLPLLLLFVTFARMAEIRPDTFGLLLFNIGWWWLLRGTGRANIL
                     LAAAFAGAALFFSARAAVMIVGLALLCAWLCVRRRDWATFLYLVLLAVGLGAIAALAY
                     VGDPAAFTRMVTSVYLDPIVLMPDLTIGQRVLSVDRLLLLAMILVALAAALLAIARGK
                     DRETAPVVAAACLTQLVLVVADPSPFQYVYGWAALPTLAGIALLGRYSVRGLHAGLAL
                     AGSALAIGLVSLSLAQAASGRPPRPGSMLRMTYDPPLPEAGLRRASTPDLVSMMVRTE
                     RQQGLWNQLALLTEICRRVRGPVLAKFYAHPICLHDLRHDWAGLKWPPMLVGDPGLGS
                     IREFEALFARRPPELVAWGKQHFTPEPTAWGRTLLKDYDIHDGFALRRAPGPRRPIAS
                     RGRR"
     gene            complement(3293..4417)
                     /gene="dapE"
                     /locus_tag="VFU20_09675"
     CDS             complement(3293..4417)
                     /gene="dapE"
                     /locus_tag="VFU20_09675"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014849301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HEU5286755.1"
                     /translation="MASDPIALASALINCPSVTPARGAVFDVLEAAVRPLGFTVHRFT
                     EGEAPDGPVENLVAIRGDGSPHFGFAGHLDVVPPGEGWDGDPFEARVADGVLTGRGAN
                     DMKSAIAAFVAALSRFEQKRGTISLLITGDEEGPSVHGTPAIIRWLDERSIRPDMILI
                     GEPTSETMLGDTVKIGRRGSVNMWIEVPGTQGHVAYPHRAANPVPPLARLVAALDALH
                     LDDGNDAFPPSNLEFTDIGCPPGATNLIPGSASARLNIRFNNLHRGADLVRLVEETAA
                     REAPGATVVAKISGEAFLTPPGELYDLVVDAIRDETGRTPTLSTGGGTSDGRYLIKLC
                     PVVDFGLPNATMHKVGESASLADIAALSRIYERIVGKVLG"
     gene            4453..4905
                     /locus_tag="VFU20_09680"
     CDS             4453..4905
                     /locus_tag="VFU20_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5286756.1"
                     /translation="MPKIDLDQVEPTNRTTYPMPFAAEMGRRHFRRLGAAAGLAEIGV
                     SHVVLEPGGVSSQRHWHEGVDEFVVVLDGEAVLVEDGGETLMRAGDCAAFPKDVANGH
                     HLVNRSEGNCTFLAIDNRRGEGDCHYPDVDLHWDQSNGRYTRKDGTPY"
     gene            complement(4912..5574)
                     /locus_tag="VFU20_09685"
     CDS             complement(4912..5574)
                     /locus_tag="VFU20_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HEU5286757.1"
                     /translation="MKLIIGNRAYSSWSMRGWLAVKQSGEPFEELVVPMFDAEWEKRR
                     EGDEFAPSLGKVPIMWDGECVVWDSLAIIEFLADRVGRARYWPEDAAARGMARSMAAE
                     MHSSFANLRRELPMNVRRSYAPRPLSDGVKEDINRILQLWAQARARFGGTGDFLFGTW
                     CAADIMYAPVVTRFITYGVAVPPFAELYMKAVLSHPHVGEWIDQAQDEPWVIEQYEDA
                     AA"
     gene            complement(5571..>5907)
                     /locus_tag="VFU20_09690"
     CDS             complement(5571..>5907)
                     /locus_tag="VFU20_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019514912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEU5286758.1"
                     /translation="DMVKRWRFLINDYLRGRAVLKRAMVLVDSRHGLKDVDREIMKML
                     DDAAVSYHLVLTKADKVKPTELANTIDAIRTEAAKHPAAHPLILPTSSETGAGIAELR
                     TAVLEAALT"
BASE COUNT          998 a         2017 c         2020 g          872 t
ORIGIN      
        1 cttcgacgcc gttaacgccg ctcgctccac attttcttaa ccgctctgga ctaatcgcgg
       61 gggcgggaga cgggcactgc cgcccgcggg cgcaattact ggcgatgaga gaagggacgg
      121 cccagaaaat cagcaacgcg ctcctgtcgg gcgcggccgg cttcgcctcg ttcgtcttct
      181 cgctgagcgc catgctctac atcaccgaac tgaacgagaa gatcgtcgcg tcgatcgtcg
      241 ccggcgcctt ctgcctgctg atcagctaca tcgcctcgga acgccccaac agcgagagcg
      301 cccgcgccct ggccgcactc ggcgaccgcc tgctcgcggt cgaggacggc gacctcgtct
      361 cgcccgctcc ggttgccgtc cagcggatca tgcccaagct cgccgccgcc gtcgacacgc
      421 tgttcgcgga agtccgctcg tcgatcgaaa acgcccatgc gctcggcatg tacgacccgg
      481 tgacctcgct cccgaaccgt ctccatttcc gttccgaagc cgacaaattg ctcagcgagg
      541 ccgacgggtc gcgatcggcg atgctgttcg tcgacctcga ccggttcaag tcggtcaacg
      601 acagcctcgg ccatgcccgc ggcgaccaat tgctgatcat ggtcgccaac cggctgcgag
      661 tcgtcgtcac cgccgaattc ggcgaggcgc tgcagcggcg gccgctgctg gcgcggctcg
      721 ccggcgacga gttcaccttg ttcttcccgg cgatccaatc ctccgccgac gtcgagcgaa
      781 tcgcccgccg gatcgctttg gcgatcgccg agccattcga gctgtgcggt cacagcgtcg
      841 atatcggcgc gtcgatcggg gtgtcgatct gccccgacca cggctcgagc atcgaatcgc
      901 tgatgcgcgc cgcggacatc gccatgtatt gcgccaagtc gaccggcggc ggccagcact
      961 gcctgttcaa cgagcaactg gcggcccagc accaggaaaa agtcgagacc gagcgggcgc
     1021 tgaccgaggc ggtccagcgc gacgagttcc tgctcgcctt ccagccgcag ctgagcctgg
     1081 tgaccgggga aatcgccggc gccgaggcgc tgcttcgctg gaaccatccg cgcgacgggc
     1141 tgcgaatgcc ggacagcttc gtgccgattg ccgagaggac cggcatgatc accgatatcg
     1201 gcgattgggt ggtcgccgaa gtcgcatcgg tgctcggctc gtggaaccgc gaggggatcg
     1261 cccgccgcct cgcgttcaac gtcagcccgc gccagatcga gcgcgccgac tttttctccc
     1321 ggctgcgcca cagctttgcc gacgagggcg tgccgctgtc gctgatcgag ctcgagttca
     1381 ccgaaagcgc ggcgatggaa gtgtcggaag gagtgctggc cgagatcgcc gcgctgcgcg
     1441 ccgacggagc gaccatctcg atcgacgatt tcggcaccgg ctattcgaac atcgcccggc
     1501 tgcggacgat gccgctcgac cgcgtcaagc tcgatccgtc gctgatcgcc gacatcgagc
     1561 attgcgagcg cgcgcgagtc gtcgtccagg cggtgatcca gctgattcac ggagtcggct
     1621 gcgagatcgt cgccgaggcg gtcgagacgg tcgcccaggc ggatatcctg cggaccatgg
     1681 ggtgccaggc gttgcagggc tatgtcttcg cccacccgat gttcgaggac gagttcgtcg
     1741 gctggatcgt caacgccgac cgcggctcgc gatcggtcgc ctaggcccgg gcgcccggcg
     1801 aagcgcgaag ccgtcgtgga tgtcatagtc cttgagtagg gtccggcccc acgcggtcgg
     1861 ctcgggcgtg aaatgctgct tgccccaggc gaccagttcg ggtgggcggc gagcgaacag
     1921 cgcctcgaac tcccgaatcg agcccaagcc cggatcgccc acgagcatcg gcggccattt
     1981 caagcccgcc cagtcatgtc gcaggtcgtg caggcagatg ggatgggcat agaatttggc
     2041 cagcaccggc ccgcgcaccc ggcggcagat ctcggtcagc agcgcgagct ggttccacag
     2101 gccctgctgg cgctcggtgc ggaccatcat cgagacgaga tcgggggttg aagcgcgccg
     2161 cagccccgct tcgggaagcg gcggatcgta ggtcatgcgg agcatcgagc cggggcgggg
     2221 cgggcggccg ctcgccgcct gggcgagcga tagcgacacc agcccgatcg ccagcgcgct
     2281 gccggcaagg gcgaggccgg cgtgcaggcc gcggaccgaa tagcggccga gcaacgcgat
     2341 cccggccagc gtcggcagcg ccgcccagcc gtagacatat tggaacggcg acgggtcggc
     2401 gacgaccagg accagctgcg tcaggcacgc cgcggcgacg accggggcgg tctcgcggtc
     2461 cttgccgcgg gcgatcgcga gcaatgcggc ggcgagcgcg acgaggatca tcgccagcag
     2521 gagcaaccgg tcgaccgaca gcacgcgctg accgatcgtc aggtcgggca tcaggacgat
     2581 cgggtcgaga tagaccgacg tcaccatccg ggtgaacgcg gcggggtcgc ccacgtaggc
     2641 cagcgcggcg atcgctccga ggccgacggc gagcagaacg aggtagagga aggtcgccca
     2701 gtcgcgccgc cggacgcaaa gccaggcgca gagcagcgca agcccgacga tcatcacggc
     2761 tgcccgggcc gagaagaaca gcgcggcgcc cgcgaaggcg gccgcgagca ggatgttggc
     2821 ccgcccggtc ccccgaagca gccaccacca gccgatgttg aacagcagca gcccgaacgt
     2881 gtccgggcgg atctccgcca ttcgggcgaa ggtgacgaac agcagcagca acggcagaag
     2941 gaacaggaaa tcgcgccggg cgagcttgag cagcaggatc gcgtaggccg ttacgacgag
     3001 cgcgctgacc gccctgagcg tccagacgaa cgacaggtcg agcggcgcag cgggcggcac
     3061 cagcactccg agaatccgga aataggccgg caggtgcgtg ctgtagaagc cggagtaagg
     3121 ctcgagcccc tcgcgcatca gccagaacac atgcgcgaaa tagatctcgt cgaccgtcag
     3181 caggccgagc aaatcgtgaa ccagcagata gtggacccag aaggcaacgg cgagaagcgc
     3241 gagcgacgtg acgatcggcg agccggggtc cgggcgggcg ccctgcatcg gctcagccca
     3301 gcaccttgcc gacgatccgc tcgtagatcc gcgacagtgc ggcgatgtcg gcgaggctcg
     3361 cgctttcgcc gaccttgtgc atggtcgcgt tgggaaggcc gaaatcgacc accgggcaca
     3421 gcttgatgag atagcgcccg tccgacgtcc cgccgccggt cgacagggtc ggggtccggc
     3481 cagtctcgtc gcggatcgca tcgacgacga ggtcgtagag ctcgccgggc ggggtcagga
     3541 acgcctcgcc ggatatcttc gcgacgacgg tcgcgccggg ggcttcgcgc gccgcggttt
     3601 cctcgaccag ccggacgagg tctgcgccgc ggtggagatt gttgaagcgg atgttgaggc
     3661 gcgcgctggc cgagccgggg atcagattgg tcgcgcccgg cgggcagccg atatcggtga
     3721 actcgaggtt cgagggcggg aaggcgtcgt tgccgtcgtc gaggtgcagc gcgtccagcg
     3781 ccgcgaccag ccgggcgagc ggcgggaccg ggttggcggc gcggtgcggg taggcgacat
     3841 ggccctgcgt tccgggcacc tcgatccaca tgttgaccga gccgcggcgg ccgatcttga
     3901 ccgtgtcgcc gagcatcgtc tccgacgtcg gctcgccgat caggatcatg tccggccgga
     3961 tcgagcgctc atcgagccag cggatgatcg ccggcgtccc gtggaccgac ggcccttcct
     4021 cgtcgccggt gatcagcagc gagatcgtgc cgcgcttctg ctcgaagcgc gacagggcgg
     4081 cgacgaacgc cgcgatcgcg cttttcatgt cgttggcgcc gcgcccggtc agcaccccgt
     4141 cggcgacccg cgcctcgaac gggtcgccat cccagccttc gccgggcgga acgacgtcga
     4201 gatggccggc aaagccgaag tgcggcgagc cgtccccgcg aatcgcgacg aggttctcga
     4261 ccggcccgtc gggcgcctcg ccctcggtaa agcggtggac cgtgaagccg agcggccgga
     4321 ccgcggcctc gagcacgtcg aacacggccc cgcgcgccgg ggtgacgctc gggcagttga
     4381 tcagagcgct cgcaagcgcg atggggtcac tcgccattcc cgccgcctag caaaggacag
     4441 ggagacagca ccatgcccaa gatcgatctc gaccaggtcg agccgaccaa ccgcaccacc
     4501 tacccgatgc cgttcgcggc cgagatgggg cggcgccatt tccggcggct gggagcggcc
     4561 gcgggccttg ccgagatcgg cgtcagccac gtcgtcctcg agcccggcgg agtctccagc
     4621 cagcgccact ggcacgaggg cgtcgacgag ttcgtcgtcg tcctcgacgg cgaggcggtg
     4681 ctggtcgagg acggcggcga gacgctgatg cgcgccggcg actgcgcggc gtttcccaag
     4741 gacgtcgcca acggccacca tctcgtcaac cgcagcgagg gcaactgcac cttcctcgcg
     4801 atcgacaacc gccgcggcga gggcgactgc cactatcccg acgtcgattt gcactgggac
     4861 cagtcgaacg gccgctacac ccgcaaggac ggcacgccct attgagccgg gctaggcggc
     4921 ggcgtcctca tattgctcga tcacccacgg ctcgtcctgg gcctggtcga tccattcgcc
     4981 gacgtgcggg tgcgacagca ccgccttcat gtagagctcg gcgaacggcg gcaccgcgac
     5041 cccgtaggtg atgaagcggg tgaccaccgg ggcgtacatg atgtcggccg cgcaccaggt
     5101 gccgaacagg aagtcgccgg tgccgccgaa ccgggcgcgg gcctgggccc acaattgcag
     5161 gatccggttg atgtcctcct tgaccccgtc cgagagcggc cgcggcgcat agctccgccg
     5221 cacgttcatc ggcagctcgc ggcgcagatt ggcgaagctc gagtgcattt ccgccgccat
     5281 cgagcgcgcc atcccgcgcg ccgccgcgtc ctcgggccag tagcgcgcgc gcccgacccg
     5341 gtcggcgagg aactcgatga tcgccaggct gtcccagacg acgcactcgc cgtcccacat
     5401 gatcggcacc ttgccgagcg acggcgcgaa ctcgtcgccc tcgcggcgct tttcccactc
     5461 ggcgtcgaac atcggcacga ccagctcctc gaacggctcg ccggactgct tgacggccag
     5521 ccagccgcgc atcgaccagc tcgagtaagc gcggttgccg atgatcagtt tcatgtgaga
     5581 gccgcttcca acaccgcagt tctcaactcg gcgatacccg ctccggtttc gctggaagtc
     5641 ggcaggatga gcgggtgggc cgccgggtgc ttggcagcct cggttcgaat cgcgtcgatc
     5701 gtgttggcca gttccgttgg cttgaccttg tcggccttgg tcagcaccag atgatagctg
     5761 accgcagcat cgtcgagcat cttcatgatt tctcgatcga cgtccttcag gccgtggcgc
     5821 gaatcgacca gcaccatcgc tcgtttcaat acggcgcgcc cacgaagata atcattgatc
     5881 aggaatcgcc agcgcttgac catgtcc
//
